LOCUS       DAWFJR010000001        71999 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWFJR010000001 DAWFJR010000000
VERSION     DAWFJR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 71999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71999
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.001"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(144..234)
                     /locus_tag="WGR04_000001"
     tRNA            complement(144..234)
                     /locus_tag="WGR04_000001"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Ser,seq:gga)
     gene            complement(273..359)
                     /locus_tag="WGR04_000002"
     tRNA            complement(273..359)
                     /locus_tag="WGR04_000002"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(323..325),aa:Leu,seq:cag)
     gene            complement(395..481)
                     /locus_tag="WGR04_000003"
     tRNA            complement(395..481)
                     /locus_tag="WGR04_000003"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Leu,seq:cag)
     gene            570..2954
                     /gene="rnr"
                     /locus_tag="WGR04_000004"
     CDS             570..2954
                     /gene="rnr"
                     /locus_tag="WGR04_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ0793651.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGIDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRQ
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAVKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIGHPFKTQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALVEQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAGKKTARGGKVRGRGASAAAESGKKAKKPIPIEVQKVKKRKGKS"
     gene            complement(3293..4756)
                     /gene="guaB"
                     /locus_tag="WGR04_000005"
     CDS             complement(3293..4756)
                     /gene="guaB"
                     /locus_tag="WGR04_000005"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ0793652.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREIILNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(4903..7461)
                     /gene="glnD"
                     /locus_tag="WGR04_000006"
     CDS             complement(4903..7461)
                     /gene="glnD"
                     /locus_tag="WGR04_000006"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ0793653.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKAKRVSVFFREYTAAVETLL
                     AALWVEHFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGGIGELCESVRDDITGDTAFLEARFLFGNRQTADKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNANRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTGEESDLLAWLVENHLLMSAVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQALLEQLT"
     gene            complement(7510..7830)
                     /locus_tag="WGR04_000007"
     CDS             complement(7510..7830)
                     /locus_tag="WGR04_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0793654.1"
                     /translation="MMGNKLTLPAELPAEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQMPS
                     ESGI"
     gene            complement(8154..8627)
                     /gene="bfr"
                     /locus_tag="WGR04_000008"
     CDS             complement(8154..8627)
                     /gene="bfr"
                     /locus_tag="WGR04_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0793655.1"
                     /translation="MKGDRLVIRELNKNLGLLLITINQYFLHARILKNWGFKELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(8655..9119)
                     /gene="bfr"
                     /locus_tag="WGR04_000009"
     CDS             complement(8655..9119)
                     /gene="bfr"
                     /locus_tag="WGR04_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0793656.1"
                     /translation="MQGNQAVLDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMVRAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(9471..9629)
                     /locus_tag="WGR04_000010"
     CDS             complement(9471..9629)
                     /locus_tag="WGR04_000010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793657.1"
                     /translation="MEFTAKATAVAGIIKPGTPRQSEERFPAPAAPFDVNNINLNCPC
                     RLKALQTA"
     gene            9841..10488
                     /locus_tag="WGR04_000011"
     CDS             9841..10488
                     /locus_tag="WGR04_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HEZ0793658.1"
                     /translation="MPIRKIPKNYRNITGIAPYNKAIGIAAYESSLERDFLTLLEFDS
                     NVQHFEVQPITIEWFDPAGKKHIYTPDVLVHYQNDVIIYEVKYRSDLRKNWCELKPKF
                     QAALHFCKLHGWHFKLITELEIHTDYLCNVRFLLPYQQNGLSGEYSEAYMTLLDESLR
                     ELKKSTPKQLIQQTFQDEYNQAKLLPVLWYLIAERKIGADLNQPLTMQSAIWFKK"
     gene            10485..12347
                     /locus_tag="WGR04_000012"
     CDS             10485..12347
                     /locus_tag="WGR04_000012"
                     /inference="COORDINATES: protein motif:HMM:NF024776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEZ0793659.1"
                     /translation="MKATLNVNVGAMVLFQNKICVITAVLELETYQLRDSVTGADYQA
                     KLADLSTVQSAELTHLDSIPEQKWQLAQQHFDVIEPLIRNPKRSKSDVQARAAECGVH
                     ISTVYRWLKSYKESGVLTALTRKQRSDSGKTQLSDDVEQLIRQVLEVDYLSTQRKSIQ
                     KIIREITRRCHKQNLPAPHANTIRSRIKNIAPQIKIAKRLSHKAAEMKFSPLQGSFPN
                     ADYPLSVVQIDHTKLDIILVDDIYRKPIGRPWITLAIDVFSRMVTGFYVSFDPPSALS
                     TGLCLAHSILSKESWLAKHGVKGRWPVWGLPRKIHLDNAKEFRGKVLEKACKQYGIEI
                     EWRPVARPHFGGHIERLLGTILDEVHGLTGTTFANTQQRKDYASESKAALTLTEFETW
                     LTLFICDVYHQRTHSGLGISPLAKWKSGVLGDDVSLGTGLPDKVVDETLLRLTFMPFE
                     MRTVQQYGVVIHKITYWHDVLRPWIAATNPTNPRQARQFIFRIDPRDLSVIWFYDPDL
                     SMYFPIPYRNSSNPVISIWELREIQRQLKNEQKQNIDENMIFTAYGRMRELEQQAQGK
                     TKAIRRAQQRRQLDQRTRASLPMPQIDSRNIISSENEENMFDDIQPFDDLDDLS"
     gene            12344..13237
                     /locus_tag="WGR04_000013"
     CDS             12344..13237
                     /locus_tag="WGR04_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HEZ0793660.1"
                     /translation="MNYPHLSPKAKEQLKYSDIERIEYIRSPRWIGYPQAQQVLVKLE
                     DLLSFPKQNRMPNMLLVGDTNNGKTMLIKHFLRQHPAHDNPHGNGIIAPVVIIQAPPI
                     PDESRFYNAILDLLFAPYKTHDRTDKKLAQVLHLLKYVNTKMLIIDEIHHILSGSLNK
                     QKTFLNVIKYLGNELQIPIVGVGIKDAYRAIQTDAQLANRFEPAVLPRWELNKDFMRL
                     LMSFERMLPLSQPSDLHEISLATKLYGMSEGYIGELSRLLTQAAVAAIQQQTECINET
                     ILNSINWISPSQRKRQLDKLR"
     gene            13234..14259
                     /locus_tag="WGR04_000014"
     CDS             13234..14259
                     /locus_tag="WGR04_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HEZ0793661.1"
                     /translation="MILPAHPKPYPDELFTSWLVRLAHANGLKVQTFCHLLFPNYEIW
                     NRDIDRHTPDWLVNTLAEKTGCSTIRIKTTTLDSLQGRLFDKNNFSGQLTWINSLKAN
                     HRKRDNNAIVYCPLCLATDHETYFRKSWRIALYTFCPIHQIMMHDCCPKCGIYIAFHR
                     QDLGKPNLQRFTELKYCWNCQYDLSNSPCENVIFFNDEIRLQWQNWLNQIANLDYLFN
                     QSEKEQLKILHHFLAITTSKKLAPDLYSYLCKQIHQIPVQLERSRRLSWESRSLAERH
                     STIHACLWLIQNFPKNLFKAWQDKAVRYNHLLKDFNDCPQSFQEIILKMNRNVHKELY
                     YSELIYY"
     gene            14320..15342
                     /locus_tag="WGR04_000015"
     CDS             14320..15342
                     /locus_tag="WGR04_000015"
                     /inference="COORDINATES: protein motif:HMM:NF012372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0793662.1"
                     /translation="MKIEAVDLFCGAGGLTAGLLRAGIKVKAGYDIDAACAYAYEYNN
                     KGAKFILQDVETVTGDQIQAHYSKDAVRLLAGCAPCQPFSTYNQGRDVRNDKKWPLLY
                     AFSRLIHEVQPELVTMENVPDVVKHEVYQDFVNNLKQQGYQVWAEKVLCADYGVPQSR
                     RRHVLLASRIGNIQMVPPTHSNCHVTVRQAIGHLPPLAAGKQDTIDVLHRCSSMSARN
                     LERIRASRPGGTWMDWPEDLRAACHRKKKGQSYKRIYGRMEWDKPAPTMTTLCIGFGN
                     GRFGHPEQDRAISLREAALFQSFPLDYQFTQETDTPIRQISRMIGNAVPVLLGEAIGN
                     SFKLLV"
     gene            complement(15326..16078)
                     /locus_tag="WGR04_000016"
     CDS             complement(15326..16078)
                     /locus_tag="WGR04_000016"
                     /inference="COORDINATES: protein motif:HMM:NF037890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793663.1"
                     /translation="MNSVKSMMESREEEINLYMDLLKYIHDSTQHGLPALQNRNGVEY
                     QLENTHLHILTASIYMLLYNYIEAVVTQSLNTLICYIQNERMPITQFSEPIQKEWLIG
                     GLNLNDAKASVETRLGNGLKMLKHFGDTQKIDIKISKGGGNFNDDEIFKLAKKLQVNM
                     CIDDETHRQLRQAGNITYESTRKFQDTCMKEITRERNKLAHGEITFQECGRNRTISDL
                     MAIKDTTFRYMHLFTDAFTEYLMQKKYLKPTV"
     gene            complement(16075..17199)
                     /locus_tag="WGR04_000017"
     CDS             complement(16075..17199)
                     /locus_tag="WGR04_000017"
                     /inference="COORDINATES: protein motif:HMM:NF015210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEZ0793664.1"
                     /translation="MKNRDTPHKKFINPTEYSLFAEEEQYDFELMIENQIKEHEKEID
                     YYVTDYPIETLVAKLDRQELFIPDYQRKNIWEKHRKSRFIESVLIGLPIPFLFFWQDP
                     STGNMEVVDGAQRLTALSEFIHGNLKLSYLEKLIYLNGQTYHQLLESRQKIFKNKPIR
                     GVVLSNKTDKEARLDLFERLNTGSKNATPAEIRRGLLQGAFYDMVETLSEDKTFNELA
                     KISKNMQDMGEREEFITRFFAYGEYLDHYSANVRKFIFDYTEIMNEEFAKNPNMRATY
                     EQKFHEIMAFFKEKYPIGFKRDGKGQSSRTRFEGMAVSAYLAEKENPGILAQVTPKEC
                     VDIFDSKEFIEVTGSDGANATKKLHARLFFAKNAFKRNHP"
     gene            complement(17471..19213)
                     /locus_tag="WGR04_000018"
     CDS             complement(17471..19213)
                     /locus_tag="WGR04_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793665.1"
                     /translation="MDQQPVIEIAEFPHDNKLWRIDGLGKIFSQLKNNNNLFIEVYLR
                     PVNFHDNSVYNFAKFDSSGREIPEYNQLKKIPVNIGLLPILRVGSLWIKGKLQNNNAV
                     TDIATLKNIEINANTVEIINSNYKIKYLDSSGREQECKLLLQKNFATDAKVPFSCFKI
                     QNENYKNGIIIPVIEIIRFYYACSTRLAHLTFSNPDEITKISYAIDNETDKVVIKLPQ
                     QYTDQEAFILARILTSNIALSGLFQIKNSLIKIGVNEQPTTLLSTNFPFINPTNLMVR
                     GIKIVDRFLVTEICSCSAPFPDISVDRNNDNRKGDKKTDIPDDKKKEYRRGGQTPSLK
                     GNSTIQNQYESVNCVSTLQFNLNINCFSDLIGKKIEKPQKPFNEYKAQKLAINEKINV
                     TSLGVDLGTWSQTTIVPASISTFNTQSEKQNRDALAATFDNSVRAIEHLNEEYSDIQA
                     TIYMSSSRACEFVPLTDSAHKAQWSYLVSKTKTRRKVLLGKIEYKDKTFWLIEIQARK
                     SEHFVTAIIYFEAIDETKYLTQLLYVLAKQRGIWDKVPKNSSLQIITLKHTHKDIKSY
                     AQKIHKKISNFILK"
     gene            complement(19276..20244)
                     /gene="lipA"
                     /locus_tag="WGR04_000019"
     CDS             complement(19276..20244)
                     /gene="lipA"
                     /locus_tag="WGR04_000019"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ0793666.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRECEF"
     gene            complement(20237..20860)
                     /gene="lipB"
                     /locus_tag="WGR04_000020"
     CDS             complement(20237..20860)
                     /gene="lipB"
                     /locus_tag="WGR04_000020"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ0793667.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(20862..21137)
                     /locus_tag="WGR04_000021"
     CDS             complement(20862..21137)
                     /locus_tag="WGR04_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ0793668.1"
                     /translation="MTEQENKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            21322..22392
                     /locus_tag="WGR04_000022"
     CDS             21322..22392
                     /locus_tag="WGR04_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ0793669.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMLAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            complement(22588..23535)
                     /locus_tag="WGR04_000023"
     CDS             complement(22588..23535)
                     /locus_tag="WGR04_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ0793670.1"
                     /translation="MEFFIILLAAVVVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(23550..23957)
                     /locus_tag="WGR04_000024"
     CDS             complement(23550..23957)
                     /locus_tag="WGR04_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ0793671.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDAGQYAEILRHAGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIIVNP"
     gene            24038..24697
                     /gene="ung"
                     /locus_tag="WGR04_000025"
     CDS             24038..24697
                     /gene="ung"
                     /locus_tag="WGR04_000025"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ0793672.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            24694..24903
                     /locus_tag="WGR04_000026"
     CDS             24694..24903
                     /locus_tag="WGR04_000026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0793673.1"
                     /translation="MNADIAVAAGVLKSRLICNFHLLGKTLWQSKKNQLYASLWAGCD
                     ELRGGMDASLYKELCAYAIVFKICF"
     gene            24800..25447
                     /locus_tag="WGR04_000027"
     CDS             24800..25447
                     /locus_tag="WGR04_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0793674.1"
                     /translation="MHHFGRAATSCAAVWMRAFIKNYVLTLLFLKYVSDKHKYGGGMI
                     ELHAGTTFDDIVKLKNTADIGDRLNKIIAQIAEANDLKGVIDVTDFNDEDKLGKGKEM
                     IDRLSRLVGIFKKLNLSSNQAEDDDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSRI
                     MAKIIGISADCRPAPAFMTRPAARVRCCSKPPPKPAAKSAFTVRKKMWQPRPLPV"
     gene            25360..25716
                     /locus_tag="WGR04_000028"
     CDS             25360..25716
                     /locus_tag="WGR04_000028"
                     /inference="COORDINATES: protein motif:HMM:NF014442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0793675.1"
                     /translation="MLKAAAQAGSQISLYGQEKDVATASLARMNMILHNNETAEINTG
                     NTLSDSSFRDENDGLKTFDFAVANPPYPARKKRRLRLFAASAQKPETKRQRCDYSSAR
                     CAVSRQCRSAYSHGIA"
     gene            25604..26080
                     /locus_tag="WGR04_000029"
     CDS             25604..26080
                     /locus_tag="WGR04_000029"
                     /inference="COORDINATES: protein motif:HMM:NF014442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEZ0793676.1"
                     /translation="MLHLLKSLKPSGKGAIILPHGVLFRGNAEARIRTELLNLDLIKG
                     IIGLPANLFYGTGIPACIIVIDKEHAQTAQFAEEGTNQVISGGSVFMIDASRGFIKDG
                     NKNRLREQDIHKIIDTFTKQEEIPRYSRMVHLSEIAAQDYNLNLPRYIDSGEVEDL"
     gene            26212..26328
                     /locus_tag="WGR04_000030"
                     /pseudo
     CDS             26212..26328
                     /locus_tag="WGR04_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            26398..27354
                     /locus_tag="WGR04_000031"
     CDS             26398..27354
                     /locus_tag="WGR04_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0793677.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAVIQPGSRPGRLIRKWSE
                     SLLDAFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEAA
                     NLTVVFEETETGKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERL
                     SQELENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMT
                     QEKAAKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQ
                     QLISRLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            27496..27630
                     /locus_tag="WGR04_000032"
     CDS             27496..27630
                     /locus_tag="WGR04_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HEZ0793678.1"
                     /translation="MTHLLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT
                     "
     gene            complement(27920..28435)
                     /locus_tag="WGR04_000033"
     CDS             complement(27920..28435)
                     /locus_tag="WGR04_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793679.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CLSTDLPPIGNYSSERYIPPQTSEPTPSPSNGGQAVKYKAPVKTVSKPAKSNTPPPQQ
                     APVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(28901..30823)
                     /locus_tag="WGR04_000034"
     CDS             complement(28901..30823)
                     /locus_tag="WGR04_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0793680.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGKITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFAGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIIVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSQLAKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(30900..31271)
                     /locus_tag="WGR04_000035"
     CDS             complement(30900..31271)
                     /locus_tag="WGR04_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEZ0793681.1"
                     /translation="MLFEENPIDGQFAEYECGAGGIRLARQSFHKPVLVHKDSVCLSQ
                     CRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGIGVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            31466..32773
                     /locus_tag="WGR04_000036"
     CDS             31466..32773
                     /locus_tag="WGR04_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ0793682.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQICPSAAFVKAPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFSLAEEKNVMVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            32766..33200
                     /locus_tag="WGR04_000037"
     CDS             32766..33200
                     /locus_tag="WGR04_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793683.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(33259..33528)
                     /locus_tag="WGR04_000038"
     CDS             complement(33259..33528)
                     /locus_tag="WGR04_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ0793684.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(33710..36172)
                     /gene="lon"
                     /locus_tag="WGR04_000039"
     CDS             complement(33710..36172)
                     /gene="lon"
                     /locus_tag="WGR04_000039"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ0793685.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLTQRQQILEISEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFSKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNVKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGVEAAAESASKPKPRSRATKH"
     gene            36257..36448
                     /locus_tag="WGR04_000040"
     CDS             36257..36448
                     /locus_tag="WGR04_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HEZ0793686.1"
                     /translation="MEFDAMPSETFRLQTAWAWKDKAGTKNRSVLPLLAVPSVWFAVL
                     PPYFQNMTQVFHVFFYFSR"
     gene            36423..37526
                     /locus_tag="WGR04_000041"
     CDS             36423..37526
                     /locus_tag="WGR04_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0793687.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNLDGEIRLIISDLAEHLHLRPSDFLDKAFAQAGLRVADVL
                     KTKPVHKKAADPNNPLAFARNRETTFLYRLKKA"
     gene            complement(37583..39328)
                     /gene="recD"
                     /locus_tag="WGR04_000042"
     CDS             complement(37583..39328)
                     /gene="recD"
                     /locus_tag="WGR04_000042"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ0793688.1"
                     /translation="MERQTDEFAQAAARAAIRFLEHYAGSGDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFDHIRPLPFDVLIVDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTILDRETYKSLNLYLPEHGFPVHEDAPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNAQVERLYRAHKAYWQAVKDGN
                     IEAAYVGISDIVVLAAWRQDSEDFNEAYCRHVRRKMNIPEHQAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEEAFRQAAATVKTR
                     QTALGSMLERVFSQG"
     gene            complement(39396..40091)
                     /gene="lolD"
                     /locus_tag="WGR04_000043"
     CDS             complement(39396..40091)
                     /gene="lolD"
                     /locus_tag="WGR04_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ0793689.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIHAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAEEAAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(40084..41331)
                     /locus_tag="WGR04_000044"
     CDS             complement(40084..41331)
                     /locus_tag="WGR04_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ0793690.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDAVWVRDWTYSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPAGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            41536..41814
                     /locus_tag="WGR04_000045"
     CDS             41536..41814
                     /locus_tag="WGR04_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793691.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(41870..42487)
                     /gene="recR"
                     /locus_tag="WGR04_000046"
     CDS             complement(41870..42487)
                     /gene="recR"
                     /locus_tag="WGR04_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ0793692.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(42554..44092)
                     /locus_tag="WGR04_000047"
     CDS             complement(42554..44092)
                     /locus_tag="WGR04_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ0793693.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGVDYIVQVGD
                     EKISEQSLNAAVQNEQAGGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(44152..44526)
                     /locus_tag="WGR04_000048"
     CDS             complement(44152..44526)
                     /locus_tag="WGR04_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ0793694.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKALFSTVEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFNRQAPCRQG"
     gene            44676..46304
                     /locus_tag="WGR04_000049"
     CDS             44676..46304
                     /locus_tag="WGR04_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ0793695.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTTGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNATMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYTTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQEGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            46376..47629
                     /locus_tag="WGR04_000050"
     CDS             46376..47629
                     /locus_tag="WGR04_000050"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ0793696.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHTDKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQM
                     VANGEADALVAERVWQELAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
     gene            47852..48160
                     /locus_tag="WGR04_000051"
     CDS             47852..48160
                     /locus_tag="WGR04_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793697.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQIRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            48242..49273
                     /gene="ruvB"
                     /locus_tag="WGR04_000052"
     CDS             48242..49273
                     /gene="ruvB"
                     /locus_tag="WGR04_000052"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ0793698.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLATIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDVQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATEHAY
                     LHFGLPVEK"
     gene            complement(49296..50024)
                     /gene="rpe"
                     /locus_tag="WGR04_000053"
     CDS             complement(49296..50024)
                     /gene="rpe"
                     /locus_tag="WGR04_000053"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ0793699.1"
                     /translation="MTAYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRRV
                     RAMLDRYEAQSGRHIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            50170..50604
                     /locus_tag="WGR04_000054"
     CDS             50170..50604
                     /locus_tag="WGR04_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ0793700.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPLNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(50617..51480)
                     /locus_tag="WGR04_000055"
     CDS             complement(50617..51480)
                     /locus_tag="WGR04_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0793701.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLSAVQSWLEDFDVPYDEAARTAALQWAQMRGNRSGRSAWQFACD
                     WTGRLPEQRAP"
     gene            complement(51488..52105)
                     /locus_tag="WGR04_000056"
     CDS             complement(51488..52105)
                     /locus_tag="WGR04_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ0793702.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFHTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(52268..52881)
                     /locus_tag="WGR04_000057"
                     /pseudo
     CDS             complement(52268..52881)
                     /locus_tag="WGR04_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
     gene            53087..54859
                     /locus_tag="WGR04_000058"
     CDS             53087..54859
                     /locus_tag="WGR04_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ0793703.1"
                     /translation="MILPARFSDGISLSLRLKLLTGLWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRAQIDNQVAEFEKSLKRIAQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFSDGISLTGDDRTLLQTLIRQLGVSLASAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENISFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQAQQGTTVSLTVASEESLK"
     gene            54856..55512
                     /locus_tag="WGR04_000059"
     CDS             54856..55512
                     /locus_tag="WGR04_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0793704.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECIRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
     gene            complement(55986..57020)
                     /gene="purM"
                     /locus_tag="WGR04_000060"
     CDS             complement(55986..57020)
                     /gene="purM"
                     /locus_tag="WGR04_000060"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ0793705.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGVGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
     gene            complement(57541..57625)
                     /locus_tag="WGR04_000061"
     tRNA            complement(57541..57625)
                     /locus_tag="WGR04_000061"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(57589..57591),aa:Leu,seq:tag)
     gene            57779..58456
                     /locus_tag="WGR04_000062"
     CDS             57779..58456
                     /locus_tag="WGR04_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793706.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGTASIERSV
                     LWLEHEDWRLADYLEQNSGNEEGLKRLFMALDSVFSRCKGVRAAAVYALMPSENAAFR
                     LVCLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNIASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKLLLGMSAG
                     KDGSEDV"
     gene            58449..59042
                     /gene="ribA"
                     /locus_tag="WGR04_000063"
     CDS             58449..59042
                     /gene="ribA"
                     /locus_tag="WGR04_000063"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ0793707.1"
                     /translation="MSEMLNHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGDFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            59210..60227
                     /locus_tag="WGR04_000064"
                     /pseudo
     CDS             59210..60227
                     /locus_tag="WGR04_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            60442..61779
                     /gene="ribB"
                     /locus_tag="WGR04_000065"
     CDS             60442..61779
                     /gene="ribB"
                     /locus_tag="WGR04_000065"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ0793708.1"
                     /translation="MTDTAGLRRHNLRQWIEKYYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHNLKIGTIT
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERVQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILVGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(61939..62076)
                     /locus_tag="WGR04_000066"
     CDS             complement(61939..62076)
                     /locus_tag="WGR04_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ0793709.1"
                     /translation="MKPVKLKMPSESLSDGILFGLENTYAVLSLKERIQKTKATGIYR
                     K"
     gene            complement(62087..63688)
                     /locus_tag="WGR04_000067"
     CDS             complement(62087..63688)
                     /locus_tag="WGR04_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0793710.1"
                     /translation="MTEQHFTEQTKSLIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKQIRKENPDEPIEFINMDIEGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAAHNAELFSVKTEGGAKIVLFERISQYITDEGRRDDFCRALISKLAGFSFEA
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMADILVPEEVRGQIRSVD
                     VYDPSAGSGTLLMNVAHAIGEDKCMIYTQDISQKSSNLLRLNLILNNLVHSLNNVVQG
                     NTILSPAHKDASGRLKKFDFIVSNPPFKLDFSDFRDQLEGEENRERFFAGIPKIKAKD
                     TDKMEIYQLFIQHILFSLKENSKAAIVLPTGFITAQSGIDKKIREYLVENKMLAGVVS
                     MPSNIFATTGTNVSILFIDKANKDEVVLIDASGLGEKIKDGKNQKTVLSREEEQKICD
                     TFTHKKAVEDFSVVVGYDEIQAKNYSLSAGQYFEVKIDYVDISADEFAQKMAGFSADL
                     DKLFAESAELEKEIKDRLAMLKFNS"
     gene            complement(63736..64893)
                     /locus_tag="WGR04_000068"
     CDS             complement(63736..64893)
                     /locus_tag="WGR04_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ0793711.1"
                     /translation="MNSALKNHATYINNKVDTNEISLSQYISTDNILQNKQGIECATS
                     LPIQGGKVTSFKKGDILLANIRPYLKKIWYAQFDGGCSADVLAIRANTETDSHFLFYA
                     LFRDDFFIHAMKGAKGTKMPRGDKTQIMEFKIPVFDLKTQQSIAAVLSTLDKKIALNK
                     QINARLEEMAKTLYDYWFVQFDFPDANGKPYKSSGGEIVFDETLKREIPKGWGSIELQ
                     SCLAKIPNTTKILNKDIKDFGKYPVVDQSQDFICGFTNDEKSILNPQDAHIIFGDHTR
                     IVKLVNFQYARGADGTQVILSNNERMPNYLFYQIINQIDLSSYGYARHFKFLKEFKII
                     LPSKDISQKYNEIANTFFVKVRNNIKQNHQLTQLRDFLLPMLMNGQVSVAE"
     gene            complement(64987..65976)
                     /locus_tag="WGR04_000069"
     CDS             complement(64987..65976)
                     /locus_tag="WGR04_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004143539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEZ0793712.1"
                     /translation="MHNNDIIIYTTEDGLSEFTLRELDGELWLTQKEIAELYQTSKQN
                     IGKHIKAIFAEQELDDSVVNFQFTTAADGKNYRMGLYPLSLIIAVGYRVRSTRGTQFR
                     QWATRTLGEYISKGFVLNDDRLKNPPVGTNRAEDQFEQLLNRIRDIRSSERRMYLRVR
                     EIFAMAADYQPSFKETTHFFKIIQNKLHYACTRYTAPEIIYQRADAGKPNMGLTHWRG
                     KEIAKADVTVAKNYLNEAEIDALNRIVSMWLDFAEDQASMKKQIFLQDWAEKLDAFLA
                     FNDRQVLQTAGTISKTQADEKARIEYEKFAAARRLEKEREGERHIAELLNLKK"
     gene            complement(66025..69030)
                     /locus_tag="WGR04_000070"
     CDS             complement(66025..69030)
                     /locus_tag="WGR04_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ0793713.1"
                     /translation="MSQTHNENSRVKIPAVLHLMRLGYDYLSLKNENWDKPTNIFPKI
                     FIESLCRINPDLSPDDARRLLTDIRLELDNEDLGQKFYERLTNQSGGKKLIDFQNFDN
                     NSFHVVTELPCINGDEEFRPDITLLVNGMPLVFIEVKKPNNKGGIGEERERMGKRAKN
                     PKFRRFINITQFMIFSNNMEYDDGATEPAQGVFYASSAYGKPVFNYFREEHKLNLAEL
                     LNTLSDDFENKVLQDNNLPVIKHSPEFVSNKSPDTPTNRILTSLLCRKRLAFLLQHGL
                     TYVKTDQGALQKHIMRYPQLFATLAIEKHLANGGKKGVIWHTQGSGKTALAYYNTRYL
                     THYYAKQGIVPKFYFIVDRLDLLKQAHQEFTDRNLIVHTINSREAFAADIKSAQTLHN
                     HAGKAEITVVNIQKFKDDPDVVARNDYDLAIQRVYFLDEVHRSYNPKGSFLANLNQSD
                     VNAVKIGLTGTPLIGVTAGNVNTRELFGDYIHKYYYNASIADGYTLRLIREEIGSRYK
                     AQLQEALAQLEIEKGSFDRKEIYAHPHFVHPMLDYILDDFAKFRKTNQDDSLGAMVVC
                     DSAEQARQLFEHFQIASDHNFTAALILHDVGTKDERDQWVKDFKAGKIDILFVYNMLL
                     TGFDAPRLKKLYLGRLIKAHNLLQTLTRVNRTYKSYRYGYVVDFADIEREFDKTNRAY
                     WDELSNELGDEIGSYSQLFKTAEEIEQEIADIKNALFDFDTENAEEFCCQISQIEDKK
                     QLLTLKKALQTAKELYNILRLQGSHEFLAHLDFDKLNLLYRETAARLDTLNLAEKLQQ
                     GDTAHLLNEALEDVYFQFVKIGEAELKLADDLKDIMRKVREGLAGNFDQGDPEYISLR
                     EELERIFKKKNLAEVGQEEMQANIATLETVYAKIKELNRQNDLLRHKYGGDAKYARIH
                     KRLRENPALYGDKLKVFNALNGVKTDTDQKVLDMEQILDNQNYFEKQMQGIVLKRFRT
                     EQQFPVQPADIQAINRLLVREYLKESGRI"
     gene            complement(69144..70454)
                     /locus_tag="WGR04_000071"
     CDS             complement(69144..70454)
                     /locus_tag="WGR04_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ0793714.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLAQILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRMAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYKQMRRFVAENSSDEVPVHLRNAPTKLMTELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEALKAK"
     gene            70766..71188
                     /locus_tag="WGR04_000072"
     CDS             70766..71188
                     /locus_tag="WGR04_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793715.1"
                     /translation="MEWVSLFSDPQRDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADSQLGYTPELLVKSNNDILFKNNFQSVDVKPHS
                     GNIHLDAKGKIQFNLNVPDVDGVQDVASGKKYRQTIVP"
     gene            71274..71771
                     /locus_tag="WGR04_000073"
     CDS             71274..71771
                     /locus_tag="WGR04_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HEZ0793716.1"
                     /translation="MNVKVHKDTHHFGIHNLNNTDVHLTAEDSDNVIEIKGELADLMP
                     TGNVESTSFLRGIETAFKSNTVLTGNNNKISIATEGKGLGLGVNGLQSNSPYSGHTTG
                     IFINNQSSDLTQPSLDGISTIKLKAVKGNNEVDLNVKNHASVKGIIASHSAKATLEAE
                     GDNIV"
     gene            71876..71962
                     /locus_tag="WGR04_000074"
                     /pseudo
     CDS             71876..71962
                     /locus_tag="WGR04_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
BASE COUNT        17543 a        17709 c        17945 g        18802 t
ORIGIN      
        1 taccgcaaaa cataccataa aaaaaacggt aacggatagt tgtaagcggt tgatgacgat
       61 tatagacaat aacgggtttt cccaatgaaa ttattgtttg aataataaaa aatcccaaac
      121 cgtaaaagtt tgggatttgt atttggcaga gaggaaggga ttcgaaccct cgatacgcta
      181 ttcacgtata cacgctttcc aggcgtgcga cttaaaccac tcatccacct ctctaatggc
      241 ggaaattatc ccaatcggga taatttatta tttggtgccc gggagaagac tcgaacttcc
      301 acacccatga ggataccagc acctgaagct ggcgcgtcta ccaatttcgc cacccgggca
      361 atctaaatat tgaaataaag caaagcattt gatttggtgc ccgggagaag actcgaactt
      421 ccacacccgt gaggatacta gcacctgaag ctagcgcgtc taccaattcc gccacccggg
      481 ctttgcttta tttgctgttt tgcggtacag tgtcctgccg caaagaagtc gctattatat
      541 agttcataaa gagaatgtca acagtccaaa tgaataaaaa tattaaatct ttaaatttac
      601 gggaaaaaga cccgttttta agtcgtgaaa aacagcgtta tgaacatcct ttgcccagtc
      661 gggaatggat aatcgaattg ttggagcgca aaggtgtgcc ttcaaaaatc gaatcgcttg
      721 cgcgcgagct gtcgattacg gaagacgagt atgtcttttt cgaacgccgt ctgaaggcga
      781 tggcgcggga cggtcaggtt ttaatcaacc gccggggcgc ggtttgcgcg gcggacaaat
      841 tggatttggt caaatgccgt gtcgaggcgc acaaagacgg cttcggtttc gccgtgccgc
      901 tcacgcccgc caaagacggt gattttgttt tgtacgaacg ccagatgcgc ggcattatgc
      961 acggcgatat tgtcactgtt cgtcctgccg gcatcgaccg taggggccgc cgcgaaggga
     1021 cagttctgga tattgtcgaa cgcgcgcaaa gcaaagtggt cggccgtttc tatatggata
     1081 ggggcgtggc gattttggag ccggaagaca agcgtctgaa ccaaagcatc gtattggaac
     1141 cggacggcgt ggcgcgtttc aaacctgaat ccggtcaggt catcgtcggc gaaattgagg
     1201 tttatcctga gcaaaaccgg caggcagtgg caaaaatcat cgaagttttg ggcgattatg
     1261 ccgacagcgg catggagatt gaaattgccg tgcgcaagca tcatttgccg caccaattca
     1321 gtgaagcgtg tgccaaagcc gtgaaaaaaa ttcccgacca tgtacgcaaa agcgatttga
     1381 aaggccgcgt cgatttgcgc gacctgcctt tggtaacgat tgacggcgaa acggcgcgcg
     1441 attttgacga tgcggtgttt gccgaaaaaa tcggacgcaa ttaccgtctg gtcgtggcga
     1501 ttgccgatgt cagccattat gtccgccccg atgacgctat cgacacggac gctcaggaac
     1561 gcagcaccag tgtttacttc ccgcgccgcg tgattcccat gttgccggaa aacctgtcca
     1621 acggcatctg ctcgctcaat cctaatgtcg agcgtttgtg tatggtgtgc gatatggtca
     1681 tcacttacgc gggcaatatt aaagaatacc gcttctaccc cgccgtgatg cgctctcatg
     1741 cccgcctgac ctacaaccaa gtttggaaat ggctttcagg cggcatcggg catccgttca
     1801 aaacacaaat cgacacgctt tacaaactct tcaaaatcct tcagaaaaag cgtttcgaac
     1861 gcggggcggt ggagtttgac agcatcgaaa cccaaatgct tttcgacgac aacggtaaaa
     1921 ttgaaaaaat cgtccccgtt gtccgcaacg atgcccacaa gctgattgaa gaatgtatgc
     1981 tggcggcgaa tgtttgcgcg gcggattttc tgttgaaaaa caagcatacg gctttgttcc
     2041 gcaaccattt gggccccacg cccgaaaaac tcgccaccct gcgcgagcag ctcggtctgt
     2101 tggggcttca acttggcggc ggcgacaacc cgtcgccgaa agactatgcc gcgcttgtcg
     2161 aacaattcaa aggcagacct gatgccgaat tgctgcaagt catgatgttg cgctccatgc
     2221 agcaggcggt ttacgaaccg cattgcgacg gacactttgg tcttgcctac gaagcatacg
     2281 cccacttcac ctcgcccatc cgccgctatc ccgacctgac cgtacaccgc gccatcaaag
     2341 ccgtgttgaa tcagcaaacc tacacgccaa aaaaaagctg gcaggctttg ggcgtgcata
     2401 cctcgttctg tgagcgccgt gccgacgacg ccagccgcga cgtggaaaac tggctgaaaa
     2461 cctattatat gcgcgataag gtcggcgaag tattcgaagg taaaatctcc ggcatgacca
     2521 gttttggtat ctttgtaaca ctggacggca tccacattga cggcttggtg catatcagcg
     2581 atttgggcga agactatttc aacttccgcc ccgaaatcat ggcaatcgaa ggcgaacgca
     2641 gcggcatccg tttcaacatg ggggacaggg ttgccgtccg ggtcgcccgt gccgatttgg
     2701 atgatggaaa aatcgatttt gtcctgattg ccgaggggag cggcaggggg cggaaagtta
     2761 aatcatccgc gtctgccaaa ccggcaggga cggcggggaa agggaaaccg aaaaccgccg
     2821 ccgggaaaaa aacagcccga ggcggcaaag taaggggaag gggcgcgtct gccgccgcag
     2881 aatcggggaa aaaggcaaag aaaccgattc cgattgaagt acaaaaggta aaaaaacgga
     2941 aaggcaaatc ataatgctga cggggcggct tggggagggc agggcatact ttcccgaagc
     3001 cgccgcccga ttttttgata tggcggccgg cgcatcattt caggccaatc gaaacgcccg
     3061 tattgaaaga ttgcgtttat ttccaccgcc gttttaaagg ccggcggtat tcggcagact
     3121 gggcgcaaac ggcgttcaga cggcattttc attctttcgg cgtgtccgtc cgaattgctt
     3181 tgcccgtccg cgcaatcagc ccgtgcggct tgccttgaac ggacaaaaaa tgccgtctga
     3241 aacccgaaaa tcaggtttca gacggcattt tgccttgaaa aagctgttca aatcagcgat
     3301 ggtagttcgg tgcttctttg gtaatttgaa cgtcgtgaac gtgcgattcg ctcatacctg
     3361 cggaagtgat ttccacaaat tcggcttttt cgtgcatttc agcaatattg gcgcaaccca
     3421 aataccccat gctggagcgc agtccgccgg tcagttggtg gatgatattc acgatcgggc
     3481 ctttgtaagg cacgcggcct tcgatgcctt cggggacgta tttgtcggtg ctgtcggttt
     3541 tgtcttggaa gtagcggtcg gcagaacctt ggctcatcgc gcccaaggaa cccataccgc
     3601 gataggattt gtacgagcgg ccttggtaga gttcgatttc gcccggcgct tcttccgtgc
     3661 ctgcaaacat accgccgagc atgacgctgt acgcgcctgc ggcgagggct ttggcgatgt
     3721 cgccggagaa gcggatgccg ccgtcggcaa tcagcggaac gcccgtgcct ttgagggctt
     3781 cggcaacgtt gtgaatggcg gtcagttgcg gcacgcccac gcctgccacg atacgggtgg
     3841 tgcaaatcga tcccggaccg ataccgactt tgacggcatc cgcgccggcg gcgaccaaat
     3901 ccaatgcggc tttggcagtg gcgatgttgc cgccgatgac ttggatgtgc ggataggttt
     3961 ctttgaccca gcgaacgcga tcaatcacgc cttggctgtg cccgtgggcg gtatcgacga
     4021 caatcacgtc cacgccggcc tcaaccaagg ctttgacgcg ctcttcggta tcgccgccgg
     4081 tgccgactgc cgcaccgacg cgcagacggc cttcggagtc tttgttggcg ttgggaaact
     4141 cggtggtttt taaaatatct ttgacggtaa tcagaccttt gagttcgtct ttttcgttca
     4201 gaaccaaaac gcgctcgact ttgtgcgtgt gcatcagttc gcgcgcttcg tctatgcttg
     4261 tgccttcggg gacggtaacc agacgttcgc gcggggtcat aatggcggaa acgggcaaat
     4321 cgacgcggtt ttcaaaacgc aggtcgcggt tggttacgat gccgacgact ttgccgtttt
     4381 caacgacggg caggccggac attttgcgtt tgcgctgcgc acgcatttcc aagacttcgc
     4441 ggatgagcgt tgtcggcgca acggttacgg ggtctttgac cacgccgctt tcgtggcgtt
     4501 tcactttgga aacggcgcgc gcctgcattt cgggcggcat atttttatgg atgatgccga
     4561 tgccgccttc ctgtgccatc gaaatggcga ggcgcgcctc ggtaacagtg tccatcgcgg
     4621 cggaaagcag ggggaggttg agtatgattt cgcgggtgag cttggtttga agtttaacgt
     4681 ctcgcggcag cacggtcgaa tgtgcgggaa ccaacaaaac atcgtcgaaa gtataggctt
     4741 tttctacgat acgcataatg ctcggtcttt cagtttgtgc aagatgcacg gcattatagc
     4801 acgttaccgg cggcttgaca gtttatattg tttgcaatat cccccgcctt cagacagcgt
     4861 acggcgcggc ggcgggatac cgtctgaaaa ccggcagggt gcttatgtca actgttccag
     4921 caaagcctgc ttcaatgagg actggatttt tgggtttttc aggtcggggc taaaaacggt
     4981 aaaactgtct tcggcgcgtt catccagtgt ggagattttg gcatagcgca ggctgacgtt
     5041 gtgggcgaaa aagacttccg ccatatcggc gagcaggaag gggcggttga cggcggtaat
     5101 ttcgacggag taccagtccg gatagtcttc ttcgggggtg atggtgatgc ttggcgcgat
     5161 cggcatgtag cggctgcggc ggctgatgcg gcggctatgg ctggagattt cggcaacggt
     5221 gtgtccgtgg ataaagctgt tgagttcggc ttcgagcgcg ctttggatgt cggggtagtc
     5281 ttcgggagcg tgctgcgagg ggatttgcac gatgaaggtg tcgaggatgt agtcgtgttc
     5341 ggtaataaag gcgcgggcgg cgaggatatc gaagccgtgg cggctgaaga tgcggcagag
     5401 gcgggcgaac aggcgcgggc cgttgggcat gaaaaccatg acttgaaagc tgtcgctttt
     5461 gggcaggatg cggctgcgga cgatgggggt ttcaaagtcg tggactaggt tggcggcgtg
     5521 ccacaggatt tcgcgggact ggtggcgggc gaagtaggcg gaaccgagcg cgttccatag
     5581 ttttttctgc tgtttttcgg ggacggcggc gcgggtgagt aagtcggcgg cttcctgccg
     5641 gcggcggccg aagagggtgt gcgggttgcc gccgttgcct gtaaggtagc gtccggcggc
     5701 atggaagagg ctttccagca ggctggcgcg ccatgcgttc cacagcttgg gattggtgcc
     5761 gcgtatgtcg gaaatggtca gaaggtagag cgcgctgagg cgttcgtggg tttggacgcg
     5821 tttgcagaaa gcatcgagta cgtcggggtc ttggatgtct tctttttggg cgacggcaga
     5881 catcagcagg tggttttcga ccagccaggc gagcaggtcg ctttcttctc cggtcaggaa
     5941 gtggtcagcg gcaaattggc gcgcgtctgc gatgccttgt atggcgtggt cgccgccgcg
     6001 tcctttggcg atgtcgtgga agaaggcggc gagatagagg atgtcttgtt tttcaaagga
     6061 ctgcatcagc gcggaggcat agggcagctc gtggctgtgc atatccaagg caaggcggcg
     6121 gacgttgcgg acgacggcga ggatgtggtc gtccacggga taaatgtgga acaggtcgtg
     6181 ctggagcagg ccgacgattt tttcccacgc gggcaggtag cgtcccaata cgccgtagag
     6241 gttgaggaag cgcagggttt gggtcagtcc gttgccgttg cggaaaaagc cggcgaagcg
     6301 gtggcggttt tcagaatttt ggtagaagct gcggttgatt ttgcgcgtcg cgccccacca
     6361 tgcgcgcagg gtttgcggtt cgagcgcggt aatatcgttg cgctgctgca tgatttcgac
     6421 gattttgaaa atgtgttcgg gccgtctgaa aaaaatatcg gtgtgccgcg cggcgatttg
     6481 gttgttgact tggatgtagt cgtcgtcaat ccgcagggta acgcgcaacg gggtggagga
     6541 aacgcggctt tgcagcatag gcgtgaggat gccgcccagt tgtttgacgg ttttaatcgc
     6601 gcggtaaaac acgcgcatca gttcttcgct ttggcggcgg aggttcaagc cttcataacc
     6661 catgctttcg gcgacttgcg gctgcaaatc gaacagcagg cggtcttcgg cgcgattggc
     6721 gtttaaatgc agatggatgc ggatgtgggc gaggcggcgg tagccgtgcg aaagcatacc
     6781 ggcttcggca cgcgtcaaaa tccgctgttt gagcagatcg ggcaggtcgg tcgccaagcc
     6841 ttgcgccttc gctatccaaa gcagggtgtg gatgtcgcgc agaccgcccg gacagctttt
     6901 gatgtttggt tccaataccg cccctgagcc ttgcgatttg gcgtggcggt gttccatctc
     6961 caccagtttt gcctcgacaa acgccgccac attgcgctgc gcgttcattt tttccgctag
     7021 tttatccgcc gtttggcggt tgccaaacaa aaacctagcc tctaaaaacg ccgtgtcccc
     7081 cgtaatatcg tcgcgcacgc tttcgcacag ttcgccaatg ccgccgcttt ttacagacgg
     7141 catcagtttg cagtcccata gggtttgaac aaaccgggca atctgttcct gaatgccgtc
     7201 tgaaagcggg gcaggggaga caaccgccaa atccacatcc gaacagggat acagttcgcc
     7261 gcgcccgaag ccgcccaccg ccatcaggca taacgcgctg ttttgaaaat gttctaccca
     7321 caatgccgcc agtaaggttt cgactgccgc cgtgtattct ctgaaaaata ccgacacgcg
     7381 cttggctttc aaataatgcg cttcggcggc atcgcgctgc tgtttgaagg tttccagtgc
     7441 tgaagacagg tttgcaggca tttttattct ttcgattggc gggaaaaaag gagacggatg
     7501 gttcggcggt caaataccgc tttcagacgg catttgtcgg gtatcggcgg aattggtcat
     7561 caggttcaac cgttcctgcg gcggcagcag caactgatac gccgccacgc ccaaagccgc
     7621 cgccattttt tcgatattcg acagggcgat gttccagcgt ttgcgttcga ctgccgacac
     7681 ataagtcctg tccaaaccgc attgccgcgc caattcttct tgcgaccaac ccttgttcac
     7741 gcggaaaagc cgcatattgt atgccaatac cgcccgcaaa tcctgttcgg caggcagttc
     7801 ggcaggcaga gtcaatttgt tgcccatcat ttgcttccgg atgaatgtta aagttaagca
     7861 tttgctgtta cggattttac ttcacataaa agccaaaaaa aaggggtggc ggcagccaca
     7921 cccaaacaca cacacatcaa gaggaaagag gtaaaatcaa gacagattgg gaaacagcaa
     7981 acaagcaatc tagatttcat atcggtatta ttaaacaaaa accgttatcg agtattgatt
     8041 caaatcaatt tagatttgat tcgcaaaccg gcaaagaaaa acggcatacc cgtttatacg
     8101 gatatgccgt ctgatgatgc aggggactgc tatatttggc agtggtttgt gttttagtcc
     8161 tcttgcgccg ctgtttgcag gtagttcggc aaaccaattt tgccgatcag ttcctgctgg
     8221 gtttcaagcc agtcgatgtg ttcttcgttg gtgtcttttt gtttttccaa caaatcacgg
     8281 ctgacgtaat cctgttgcgc ttctgctgta gcgatggcag caagcagggc ttcgtgtttt
     8341 tcctgttctt tggtcaaatc gcaggcaatg atttcttcgg tagattcacc aatcaacagt
     8401 ttgcccagtt cttgcaggtt cggcaggcct tcgaggaaca gaatgcgctc gatcaaatcg
     8461 tctgcagctt tcatttcaac gatggactgt ttgaagaaat gttcgcccag ttctttaaag
     8521 ccccagtttt tcaaaatacg ggcgtgaagg aaatattggt tgatggttat cagcagcaag
     8581 cctaagtttt tgttcagctc gcggataact aaacggtcgc ctttcatacg gactcctttt
     8641 attccgaata cagcttacag ttggctttgg tagtagttgc cttcgccaat cagctcgatc
     8701 aggcgcagct gctgttccaa ccagtgtgcg tggtcttctt cggtatcttt cagttgggca
     8761 accatcaggt cgcgcgtaac atagtcttga gcctcttcgc acagtttgat gccttttttc
     8821 aacgcatcac gtacttcata ttcggtttgc aggtcggctt tgaggcagga aaccacgtcc
     8881 gtgccgatat tcagttcggc gcgtaccatt ttcggcgtac cgcccagcat caggatgcgg
     8941 cggataaagt cttcggcgtg tgtggtttct tcttccatct cgtgattgag acgttcaaaa
     9001 agtttggtgt agccccattc ggagtagaga cgggagtgga tgaagtattg gtcgcgtgct
     9061 gccagctcac cggacagcaa ttcgttcatg taatctaaaa ctgcttgatt gccttgcata
     9121 atatttctct tttcttaatt tgggtttcgt gtggttaaac agtggatgaa gcactgttcg
     9181 ggatgcgtgc attgtatgca aaagctgtcg gcatgacaaa ttttctattt aaaatacaaa
     9241 caattatcaa aagaaatagg gcgtattccc cgatgccttc caaatcaatg ctctgtttct
     9301 tatcgttttt tttgctgcta aaactaagaa tccatctcat caataaagaa catttacact
     9361 tattaaccat agcaaaaaac aaatagagca cggtttttta tcaaaattta taatgaatcg
     9421 ttctcattaa ctgacagatt cttttgagat tatcggattt tgggaataaa tcatgccgtc
     9481 tgaagggctt tcagacggca tggacaattc aaattgatgt tgtttacatc aaacggcgcg
     9541 gcaggcgcag ggaatcgttc ttcactttgg cgcggtgttc cgggtttaat gatgcccgca
     9601 accgccgtgg ctttcgctgt gaattccaac gttgtttgcg actagaatat gcaaaatctt
     9661 atacttctaa taacgttatt tgcgactgag agcaaaaatt ttgcattaag atttgaaaat
     9721 aatattgtta tttgagacta aattataaga aagctaattg agtttgggtt attgataaag
     9781 ggcattaact cattaagatt gtcttccttt taatcctttt tctactacac atataaaact
     9841 atgccaatcc gtaaaattcc taaaaactat cgaaatatta caggcatcgc tccatataac
     9901 aaagctatag gcattgcagc ttatgagtca tctttggagc gtgatttttt aactctgctg
     9961 gaatttgatt ctaatgtgca gcatttcgaa gtacagccta tcacgattga gtggtttgac
    10021 ccagcaggaa agaagcatat ctatacccct gatgttttgg ttcattatca aaatgatgta
    10081 attatttacg aagtaaaata tcgtagcgat ttacgtaaaa actggtgtga actcaaacct
    10141 aaatttcagg ctgccttaca tttttgcaaa ttacacggtt ggcattttaa actgattaca
    10201 gagttagaga ttcatactga ttatttatgc aatgtacgct ttttattgcc atatcaacaa
    10261 aatggcttat ctggcgaata cagcgaagca tatatgactt tattggatga atcgttacgc
    10321 gaattgaaaa aatccacacc taaacagctt attcaacaga cttttcagga tgaatacaat
    10381 caagcaaaat tattgcctgt attgtggtac ttaattgcag aacgaaaaat tggcgcagat
    10441 ttaaatcaac cactcactat gcaatccgct atatggttta aaaaatgaaa gcaactttaa
    10501 atgtgaatgt cggtgcaatg gttttgtttc aaaacaaaat ctgtgtaatt acagcagtcc
    10561 tggagttaga aacatatcag ttacgcgata gtgtaaccgg tgcagattac caagcaaaat
    10621 tagcggatct atcaacagtc cagagtgctg aattaaccca cttagatagt attcctgagc
    10681 aaaaatggca attagctcaa caacatttcg atgtaattga gccacttatc cgaaatccaa
    10741 aacgtagtaa atctgatgtt caagcgagag ctgccgaatg cggtgtacat atcagtacgg
    10801 tttatcgctg gttaaaaagt tataaggaaa gcggcgttct gactgcttta acacgtaagc
    10861 agcgtagtga tagtggtaaa acacagcttt ctgatgatgt agaacagctt atcaggcaag
    10921 tattggaagt ggattattta agcacacaac gtaaatcaat acagaaaatc attagagaaa
    10981 ttacacggcg ttgccataaa caaaatttac cagctcctca tgctaatacc attcgttctc
    11041 gaatcaaaaa tattgcgcct caaattaaaa ttgctaaacg actaagccat aaagcagcag
    11101 aaatgaaatt ttctccttta caaggtagct ttccaaatgc cgattatccg ctttcagtgg
    11161 tacaaataga tcatacaaag ttagacatta ttttagttga cgatatatat cgtaagccta
    11221 ttggtaggcc gtggattacg ctggcgattg atgtgttcag tcgaatggtt acgggatttt
    11281 atgtgtcatt cgatccacca agtgcattat ctacaggttt gtgtttagca catagtattt
    11341 tgtctaagga aagctggttg gcaaaacatg gagtgaaagg gcggtggcct gtgtggggtt
    11401 tgccacgcaa aattcatttg gataatgcaa aagaattccg tggcaaagtg ctggaaaagg
    11461 cgtgtaaaca atacggtatt gaaattgagt ggaggcctgt ggcacgtcct catttcggag
    11521 gacacattga gcgtttacta ggtacgattt tggatgaagt ccacggttta actggtacta
    11581 cttttgcaaa tacacaacag agaaaagatt atgcttccga aagcaaggcg gcattaacgc
    11641 taactgaatt tgaaacgtgg ttaacactat ttatttgtga tgtttatcat cagcgtactc
    11701 attctggatt ggggatctca ccgttagcca aatggaaatc aggtgtatta ggagatgatg
    11761 tttctttagg aacaggatta cctgataaag tggttgatga gacactattg cgcttgacct
    11821 ttatgccgtt tgaaatgcga acggtgcagc aatatggggt agttatccat aaaatcactt
    11881 attggcatga cgttttacga ccatggattg cagcaactaa tccgactaat cctagacagg
    11941 ctcgccagtt catttttcgc atagatccac gtgatttaag cgtgatttgg ttttatgacc
    12001 cagatttatc gatgtatttt ccaatacctt atcggaacag ttcgaatcca gttatcagca
    12061 tttgggagtt acgagagatt cagagacaat taaaaaatga gcagaaacag aatattgatg
    12121 agaacatgat ttttacagca tacggacgaa tgcgagaatt ggaacagcaa gctcaaggta
    12181 agactaaagc tatccgtcgc gctcaacaac gtcgccaatt agatcaacgt actcgagcat
    12241 ctttacctat gcctcaaatt gattcgagga atattatatc gtctgaaaat gaggaaaata
    12301 tgtttgatga tattcagcct tttgatgatt tggacgattt atcatgaatt atcctcacct
    12361 ttctcccaaa gcaaaagaac aactaaagta tagcgatatt gagcgcatcg aatacatacg
    12421 ttcccctcgt tggataggtt atccgcaggc tcaacaggtt ttggtaaaac tggaagattt
    12481 gctaagtttc ccgaaacaaa accgtatgcc taatatgttg ttggttggtg atactaataa
    12541 cggtaaaacc atgttgatta aacatttttt gcggcaacat ccagcccatg ataatccaca
    12601 tggaaatggt attatcgcac cagtagtgat tattcaagca ccgcctatac ctgatgaaag
    12661 ccgtttttac aatgctattt tagatttact gtttgccccc tataaaactc atgacaggac
    12721 agataaaaaa cttgcccaag tgcttcattt gctcaaatac gttaatacta aaatgttgat
    12781 tattgatgaa atccaccata tcctttccgg gagtttgaat aagcagaaaa cgtttttaaa
    12841 tgtaattaaa tacttaggaa atgaattaca aattccgatt gtaggtgtag gtattaaaga
    12901 cgcatatcga gccattcaaa ctgatgcaca gttggccaac cgttttgaac ctgcggtgct
    12961 tcctcgttgg gaattaaata aggattttat gcgtttattg atgagctttg aacggatgct
    13021 gccgcttagt cagccgtcgg atttacatga aatttcgttg gctacaaagt tatatggcat
    13081 gagtgaagga tatattggag agctttctcg tttgctgaca caagctgctg tagccgccat
    13141 tcaacaacaa actgaatgta ttaacgaaac aatcttaaat tcaatcaact ggattagtcc
    13201 aagccaacgt aaacgtcaat tggataaact cagatgattt tacccgctca tccgaaacct
    13261 tatcctgatg aattgtttac atcatggttg gttcgcttag cacatgcaaa cgggttgaaa
    13321 gtacaaacct tctgtcattt gctatttcct aattatgaga tatggaatag ggatatagat
    13381 cgccatactc ctgactggtt ggtcaataca ttggctgaaa aaacaggctg ttcaaccatt
    13441 cgaatcaaaa caaccacatt agatagccta caaggtcgat tatttgataa aaacaatttt
    13501 tcaggacaat tgacgtggat aaacagcctt aaagccaatc accgtaaacg cgataataat
    13561 gctattgttt attgcccgct atgtctagct actgaccatg aaacatattt ccgaaaatca
    13621 tggcgcatag ccctatatac attttgtcca atacatcaaa tcatgatgca tgattgctgt
    13681 cctaaatgtg gcatatatat tgcttttcac cgacaagatt taggtaaacc taatttgcag
    13741 cgctttacag agttgaaata ttgttggaat tgtcaatatg acttgagcaa cagtccttgt
    13801 gagaatgtta ttttctttaa tgatgaaatc aggctgcaat ggcaaaattg gttaaaccaa
    13861 attgctaatc ttgattatct ttttaaccaa tctgaaaaag aacaactaaa aattctgcat
    13921 cactttttgg cgattactac atcaaagaag cttgcaccgg atttgtactc ttatttgtgc
    13981 aagcaaatac atcagattcc agttcaatta gaacgttctc gtcgactgag ttgggaaagt
    14041 cgcagtttgg cggaacgaca cagtactatt catgcttgtt tgtggttaat acaaaatttt
    14101 ccaaaaaatc tatttaaagc atggcaagat aaagccgtcc gttataacca tttattgaag
    14161 gattttaatg attgccccca aagttttcag gaaataattt taaaaatgaa tagaaacgtt
    14221 cataaggaac tgtactattc agagcttata tactattaaa agctcgactc caatcaaagt
    14281 taaggatata atccttatat caaaaatatg agttgaaaaa tgaaaattga agcagtagat
    14341 ttattttgtg gagcaggtgg gttgacagca ggtttacttc gagcaggaat taaagtgaaa
    14401 gctggatatg atattgatgc agcttgtgca tatgcgtacg agtataacaa taaaggtgca
    14461 aaatttatat tacaagatgt ggaaacagtt actggagatc agattcaggc gcactattca
    14521 aaagatgcag ttagattatt agctggttgt gctccttgtc agccattttc tacttacaat
    14581 caaggtcgtg atgttcgtaa tgacaaaaaa tggccattac tatacgcttt ttctcgcttg
    14641 attcatgaag tgcaaccaga gcttgtgact atggagaatg tgccagatgt tgtaaaacat
    14701 gaggtatacc aagattttgt aaataattta aaacaacagg gataccaagt ttgggctgaa
    14761 aaagtattat gtgcagatta tggagtccct caatcacgac gtagacacgt attacttgca
    14821 tcacgtatcg gtaatattca gatggttcct ccaacgcatt caaattgcca tgtaacagtt
    14881 cgtcaggcta ttggacactt gccgccactg gcagcaggca aacaagatac tatagatgta
    14941 ttacatcggt gttcatcaat gtcagctcgt aatctagaac ggattcgtgc ttctcgtcca
    15001 ggaggaacat ggatggattg gccagaagat ttacgagccg catgtcatcg caaaaaaaaa
    15061 ggacagtctt ataaacgaat ttatggtcgt atggaatggg acaagcccgc cccaacaatg
    15121 acaactttat gtattggctt cggcaatgga cgctttgggc atccggaaca agaccgtgca
    15181 atttctttaa gggaggcagc cttattccaa agttttcctc tagattacca gttcactcaa
    15241 gaaactgata ctccaattcg ccaaattagt cgaatgattg gtaatgctgt tcctgtattg
    15301 ctaggtgagg caatcggaaa tagttttaaa ctgttggttt gagatatttc ttctgcatta
    15361 gatattcagt aaatgcatca gtaaataaat gcatatagcg gaaagtagtg tcttttattg
    15421 ccattaaatc agatattgtt cggtttctac cacattcttg aaaggtgatt tctccatgag
    15481 caagtttatt tcgttcacgg gttatttctt tcatacaagt atcttgaaat ttgcgcgtac
    15541 tttcataggt aatatttcct gcttgccgta attggcggtg agtttcgtca tcgatacaca
    15601 tattaacttg aagtttcttt gcaagcttaa atatttcgtc atcattaaaa tttcctcctc
    15661 ctttagaaat cttaatatct attttttgtg tatcaccgaa atgtttaagc attttgagtc
    15721 cattacctaa tcgtgtttct acactagctt ttgcatcatt aaggtttaac cctcctatta
    15781 gccattcttt ttggatgggt tctgaaaatt gggtaatagg catcctttca ttttgaatat
    15841 agcaaattaa agtatttagg ctttgggtaa caacagcctc aatgtagtta tatagtaaca
    15901 tatagatact agcggtaagg atatggagat gagtattttc caattgatat tcaacaccat
    15961 ttctattttg taaagctggt aaaccatgtt gagtactatc atggatatac tttagcagat
    16021 ccatatatag gtttatttcc tcttcacgac tttccatcat agattttact gagttcatgg
    16081 atgattccgt ttaaaggcat ttttagcaaa aaataatcta gcatgtagct ttttagtggc
    16141 atttgcacca tcactaccag ttacttcaat gaattcttta ctgtcaaaaa tatcaacgca
    16201 ctcctttggg gtaacttgag ctaaaatgcc tggattttcc ttttcagcga gataagctga
    16261 aacagccatg ccttcgaaac gagtacgaga agactgaccc tttccatctc ttttaaaacc
    16321 aattggatat ttttctttga aaaaagccat aatttcatgg aatttttgtt cgtaagtcgc
    16381 acgcatgttt ggatttttag caaattcttc attcatgatt tctgtataat caaagataaa
    16441 tttacgaaca ttagcagaat aatggtctaa atactcacca taggcaaaaa agcgagtaat
    16501 gaattcttct cgctccccca tatcctgcat attcttgcta atttttgcta attcattaaa
    16561 tgttttatct tcagataaag tctcaaccat atcgtaaaat gctccttgaa gcagacctcg
    16621 acgaatttct gcaggtgtag catttttgct acctgtatta agacgttcaa ataagtcaag
    16681 tcgagcttct ttgtctgttt tattactaag tactacgcct cggataggtt tatttttgaa
    16741 aatcttttga cgactttcta ggagttggtg ataagtttgg ccattaagat aaataagctt
    16801 ttccaaatag gataatttta aattgccatg aataaattca gacaatgctg ttagtcgttg
    16861 tgcaccatct accacttcca tatttccagt tgaaggatcc tgccaaaaaa ataaaaatgg
    16921 gataggtaag ccaattaaaa cagactcaat gaacctactt ttacgatgtt tttcccaaat
    16981 gttttttctc tggtagtctg gaataaacaa ttcttgtcga tctaatttag caactagtgt
    17041 ttctatggga taatcagtta catagtaatc aatttctttt tcatgttctt ttatctgatt
    17101 ttcaatcatt aattcaaaat catattgttc ttcttctgca aatagactat attccgtagg
    17161 gttgataaat tttttatgcg gagtatctcg atttttcatt ttgaatacct aaaaatagaa
    17221 attgttaata ttatgttata gattaaaaac ataaccttga aatatcaaaa gaaccgtttt
    17281 cttttgtgtc tttagaattt actcttacct taacatttat gttagcagat gggaaccatt
    17341 actcctatat agtagttacg acaccaccag ccttatcaat aagagagtag gtaacttaaa
    17401 ttttatataa aagtatcagc ctgaagtatt ttggtaattt ataactagat tagttatatt
    17461 actaactaat ttattttaag ataaaatttg atattttttt gtgtattttt tgagcgtagc
    17521 tcttgatatc tttatgcgta tgctttagag taataatttg caaactggag ttttttggaa
    17581 ctttatccca aattccccgc tgttttgcta agacatataa taattgagtc aaatactttg
    17641 tctcgtcaat tgcctcaaaa taaataatcg ccgtaacaaa atgctcactt ttccgagctt
    17701 gaatttcaat caaccaaaat gttttatctt tgtactcaat ttttcctaat aataccttac
    17761 gccttgtttt ggttttggaa acaagatacg accattgggc tttatgggct gagtcagtta
    17821 atggaacaaa ctcacaagct cgagagctag acatgtagat agttgcctga atatctgaat
    17881 attcttcatt caaatgttca atagcacgta cggaattgtc aaaagtcgca gctaaggcat
    17941 ccctattttg tttttcagat tgggtattaa aagtgctaat ggacgcaggc acaattgtag
    18001 tttggctcca tgtacctaaa tccactccta agctagttac attaattttt tcatttatgg
    18061 caagtttctg tgctttgtat tcattaaagg gcttttgggg tttttcaatc tttttaccga
    18121 tcaaatctga aaagcaattt atgtttaagt taaattgtaa tgttgatacg caattgactg
    18181 attcatattg attttgaata gtggaatttc ccttgagact aggagtttgt ccccctcttc
    18241 gatattcttt ctttttatca tcaggtatat ctgttttttt atctcccttt cgattgtcat
    18301 tattcctatc aacagaaata tcgggaaaag gagccgaaca agaacaaatc tccgtaacta
    18361 agaatctatc aacaatttta ataccgcgaa ccattaaatt ggttggattg ataaatggga
    18421 aattggtaga taataatgta gttggttgtt cattaactcc tatttttatt agggaatttt
    18481 taatctgaaa gagtccagat aaagcaatat ttgaagtcaa tattcttgct aaaataaagg
    18541 cttcttgatc tgtatattgc tgtggtaatt tgatgactac cttgtctgtt tcattatcta
    18601 tcgcatatga gatttttgta atttcgtctg gattagaaaa tgttaaatga gctagtctag
    18661 ttgaacaggc atagtaaaaa cggataattt ctattactgg aataattatt ccatttttgt
    18721 aattttcatt ttgaattttg aagcaagaaa aaggtacttt tgcatccgtt gcaaaatttt
    18781 tctgcaacaa tagtttgcac tcttgttctc ttccgcttga atctaaatat tttattttgt
    18841 aattgctatt aattatttct actgtatttg catttatttc aatattttta agagtggcta
    18901 tatctgtaac tgcattattg ttttgaagtt ttccttttat ccaaagagat cccactctta
    18961 aaataggaag caagcctata ttaacaggaa tctttttcaa ttgattatat tctggaattt
    19021 ctctaccaga actatcaaat ttagcaaagt tatatacaga attatcgtga aagttaactg
    19081 ggcgtaaata gacttcaata aacaggttat tgttattttt caattgggaa aaaatcttcc
    19141 ctaacccatc aattcgccac aatttattat catgtgggaa ttcggcaatt tcaataactg
    19201 gttgttgatc catatttgcc cagtcgaact tagcgttata aagtcgcaaa tagcgtagtt
    19261 cagtctcaaa tagcgtcaga attcacattc gcgtaaggct tcggcagcct gttcgtcggc
    19321 gtggtagctg gaacgtacca tcgcgccgat ggcggcattg ctgaagccca gttcgtatgc
    19381 ttctttttca aagattttga actgctcggg cgtaacgtag cgcaggacgg gcaggtgtcc
    19441 gtctgaaggc tggaggtact gtccgatggt aatcatttcg atattgtgcg cccgcatatc
    19501 gcgcataatt tcacgcacgt cttcgtctgt ttcgcccaag ccgaccatga tgccggattt
    19561 ggtcgggatg tgcggcatca tttctttata acgttttaat aagtctaaag aatgttgata
    19621 attggcaccg ggacgggctt ttctgtacag gctcggatgg gtttctaggt tgtggttcat
    19681 cacgtcgggc ggggtttcgg caaggatttt gagtgcgatg tccaagcgtc ctcggaagtc
    19741 ggggacgagg atttcgattt tggtgttcgg gctggtttcg cggatggctt tgatgcagtc
    19801 ggcgaaatgc tgtgcgccgc cgtcgcgcag gtcgtcgcgg tcgacggagg tgatgacgac
    19861 gtaacgcagg ttcatggctt tgacggattc ggcgaggttt ttcggttcgt cggggtcgag
    19921 catattcggg cggccgtgtc ccacgtcgca gaacgggcag cggcgggtgc agatgtcgcc
    19981 cataatcatg aaggtcgccg tgcctttgct gaagcattcg ccgatgttgg ggcaggaggc
    20041 ttcctcgcaa acggtgtgca ttttttgttc gcgcaaaatg tctttgattt caaagaattt
    20101 gcgcgatgga agtttggcgc gtatccattc gggctttttt aatttttcct gaagggggac
    20161 gactttgatg gggatgcggg cggttttgtc cgcgcctctg agtttgatgc cgcgtttggg
    20221 gtcgtcggtt ttgatttcac tcattgttgt ctgctttcgg tgtgaattgt gtttcaaggt
    20281 gtgcggtaag tttggcggcg acttcgtccg gcgtggggca gggttggaca aaatccgcga
    20341 tttgcgtcat ttccataccg gcgtagccgc aggggttgat gtgggtaaac gggcttaaat
    20401 ccatattgac gttgagcgca agcccgtgat agacggagcc gtttttgata cgcagcccca
    20461 gtgaggcgat tttgcgttct ccgacataaa cgccggggcg tttggggtct gccgccgctt
    20521 cgatgccgta ttctgccaat gtggcgatga tgctgttttc aagcgcggaa acgatgttgc
    20581 ggacgctggt tttgcgccgt ttgaaatcaa tcatcgtata aacgaccaat tgcccgggcc
    20641 cgtgataggt aatctgcccg ccccggtcga tttggacgac gggaatgtcg tcgcgaatca
    20701 gcaggtgttc gggttttccc gccagtcctt gtgtgaacac gggcgggtgt tcgacgaccc
    20761 acagttcgtc ttcggtgtcg gcattccgtc cggcattaaa ggttttcatc gcttcaaaag
    20821 tcggcagata ttcgaccaaa cctttgtgta tgattttcat ctcaaagtac cactttgacc
    20881 agttcgtgcg aagtcagcgc gcggtagatg ttgtccaatt gttcttggtt ttcaaccttt
    20941 acctgaacgg tggcgccagt atagttgcct ttgctgctcg gacgcgtggt gatgtggtgc
    21001 gcctgcgtgt cgggggcgtg gaggcggacg gtgtccaata ccgcctgctc gaactcggga
    21061 tgaaccgccc ccattacttt taatgggaag gtgcagggaa attcgatgag ggatgttttg
    21121 ttttcttgtt cggtcatgat gtgctgcctt gttgtgtacg gtatgccgtc tgaaggcggg
    21181 tttgcctttc agacggcatc ggatgtgcgt tattttagcc taaaccgcga taacaggcta
    21241 tcgggaaggc ggaggctttt ttgacggcgc ggcggttctg ctatactggc gcgcaatatt
    21301 atttttagaa gggtggtttt tatgtatcgg aggaaagggc ggggcatcaa gccgtggatg
    21361 ggtgccggtg cggcgtttgc cgccttggtt tggctggttt tcgcgctcgg cgatactttg
    21421 actccgtttg cggttgcggc ggtgctggcg tatgtattgg accctttggt cgaatggttg
    21481 cagaaaaagg gtttgaaccg tgcatccgct tcgatgtctg tgatggtgtt ttccttgatt
    21541 ttgttgttgg cattattgtt gattatcgtc cctatgctgg tcgggcagtt caacaatttg
    21601 gcatcgcgcc tgccccaatt aatcggtttt atgcagaaca cgctgctgcc gtggttgaaa
    21661 aatacaatcg gcggatatgt ggaaatcgat caggcatcta ttattgcgtg gcttcaggcg
    21721 catacgggag agttgagcaa cgcgcttaag gcgtggtttc ccgttttgat gaggcagggc
    21781 ggcaatattg tcagcagtat cggcaacctg ctgctgcttc ccttgctgct ttactatttc
    21841 ctgctggatt ggcagcggtg gtcgtgcggc attgccaaac tggttccgag gcgttttgcc
    21901 ggtgcttata cgcgcattac aggcaatttg aacgaggtat tgggcgaatt tttgcgcggg
    21961 cagcttctgg tgatgctgat tatgggtttg gtttacggct tggggttggt gctggtcggg
    22021 ctggattcgg ggtttgccat cggtatgctt gccggcattt tggtttttgt tccctatttg
    22081 ggcgcgttta caggactgct gctggcaacc gtcgccgcct tgctccagtt cggttcgtgg
    22141 aacggcatcc tatcggtttg ggcggttttt gccgtaggac agtttctcga aagttttttc
    22201 attacgccga aaatcgtggg agaccgtatc gggctgtcgc cgttttgggt tatcttttcg
    22261 ctgatggcgt tcgggcagct gatgggcttt gtcggaatgt tggccggatt gcctttggcc
    22321 gccgtaacct tggtcttgct tcgcgagggc gtgcagaaat attttgccgg cagtttttac
    22381 cggggcaggt aggcggttcc gaaacatata gtggattaac aaaaatcagg acaaggcgac
    22441 gaagccgcag acagtacaaa taggacaacg ccgtactggt ttttgttaat ccactatatt
    22501 tgaagcggaa tacaaccctg cccgagttta aataaaaatg ccgtctgaac cccgaaaaca
    22561 gagcttcaga cggcatttca tcacggctta tttggcggtt ttgctgctgt cgataatttt
    22621 cataccggca gaaatcaggc tgccgatgtc ggcaacattg gcgggcataa tcagcgtatt
    22681 gctttctttg gcaagattgt tgaacgcggc gacgtattgt tccgcaatct tcagattgac
    22741 cgcatccgca ccgccttggg tttgaagggc ggcggcaatt tgacggatgg cttcggcatt
    22801 ggcttcggca acaaggcgca aggattccgc ttcacctttg gcgcggttga tgcgggcgat
    22861 tttttcggca tttgacgcat tgaccgcagc ctgagcctcg ccttcggatt gttggatttc
    22921 ggcttcgcgc tgaccactgg caaggttgat ttgttcgatt ttacgacctt cggattcggc
    22981 gatacgggcg cgtttttcgc gttcggcagt aatttgcgcc tgcattgagc gaaggatttc
    23041 ttgcggcgga accaagtctt taatctcata acgcaaaacc ttcacacccc aagccccggc
    23101 cgcctcgtcc aaagccgcaa caacagtact gttgatttcg tcgcgttctt caaacgtttt
    23161 gtccaattcc atacgcccga taacggaacg cagcgtcgtt tgggcaagct gggtaatcgc
    23221 cataatgtag ttgctcgaac cgtatgaggc gagtttgggg tcggttactt ggaaatagat
    23281 gataccgtca accgtcagct gcgtattgtc gcgcgtgatg cagacctggc tgggtacgtc
    23341 taaagggatt tctttcagcg aatggcggta ggcgacgcgg tcgataaagg gaatcaaaat
    23401 attcaaaccg gccgtcaggg cgcgatggaa acgcccgagc ctttcgacaa cgtggacttc
    23461 ctgctgtggg atgacaacaa aggatttgaa gccgaaaaca acgacggctg ccagcaagat
    23521 aatgaaaaat tccataattc ctccgagtgt taagggttta cgatgataag aagattgcct
    23581 tccttgcgga cgatgagggc gcgcgttccc ggttcaagct cttcttgccc cgtattttgc
    23641 gcctgccagt gcgtaccgcg ataaaaaact tcgtaacggt tgccgcctgc gtgccggagg
    23701 atttcggcat attgcccggc atccaaatcc tgatatgaat ccgtttcaac ttttcccacg
    23761 gcggttttgg cgtgtacgaa ccaaataccc agcgcggaaa gcagagcggc ggtcaagacg
    23821 gcggcaggcg tgctgccggt cagcccgtaa gcaatgcccg aacccgccaa agccgcgctg
    23881 acaaccaaaa gataaaccgt tcccgtcaat aattcgatga ttaagacggc aacagcggca
    23941 acaaaccata cagtcataca tttccccaca aagcgcgtcg tttgacaaaa taacgcaata
    24001 tcagcagtat agccgaattt gaaaggatag ggcagatatg gacacttggc acgatgcact
    24061 cggcggcgaa aaacagcagc cgtattttca ggaaatttta aatgcagtca ggcaggaacg
    24121 tttgtcggga caaatcatct atccgccggc ggcggatgtg ttcaacgcat tccgcctgac
    24181 agcgttcgac cgggtcaaag ccgtcattct cggacaagat ccgtatcacg gggcagggca
    24241 ggcgcacggt ttggcatttt ccgtccggca gggtatccgc ataccgccgt ctttactcaa
    24301 tatctacaaa gaattggaaa ccgacatcga aggcttttcc atacccgcgc acggctgcct
    24361 gacagcgtgg gcggagcagg gcgtattact tctgaacacg gttttgacgg tgcgtgcagg
    24421 acaggcgcat tcgcacgccc ttttaggctg ggaacgcttt accgataccg tcatcaggca
    24481 gcttgcgaca caccgcaagc atcttgtctt tatgctgtgg ggtgggtatg cacaacaaaa
    24541 aaggaaactg atagacagtc aaaaccattt gatattgacc gcaccgcatc cgtctcctct
    24601 gtcggcatat cgcggttttt tcggttgccg ccatttttca caggcaaaca gctatttgag
    24661 ccggcacggt atcgatccga taaactggaa gctgtgaatg ccgatatagc cgttgccgcc
    24721 ggcgtgttaa aatcgcgttt gatttgtaat ttccatttat taggcaaaac cttatggcaa
    24781 tcaaaaaaaa atcagcttta tgcatcactt tgggcgggct gcgacgagtt gcgcggcggt
    24841 atggatgcga gcctttataa agaactatgt gcttacgcta ttgtttttaa aatatgtttc
    24901 tgataagcat aagtacggcg gcggcatgat tgagctgcac gccggtacga cttttgacga
    24961 catcgtcaaa ctcaaaaaca ccgccgacat cggcgaccgc ctgaataaga ttatcgccca
    25021 aattgccgaa gccaacgact taaaaggcgt gatcgacgtt accgacttca acgacgaaga
    25081 caaactgggt aaaggtaagg agatgatcga ccgtttgagc aggcttgtcg gcatttttaa
    25141 aaagctcaac ctttcttcca accaagccga agacgacgat ttgttaggtg atgcctacga
    25201 atacctgatg cgccattttg cgaccgagtc aggcaaatcc aaagggcagt tttacacgcc
    25261 tgccgaagtc tcccgcatta tggcgaagat tatcggaatc agcgcagatt gccgtccagc
    25321 accagcgttt atgacccgac ctgcggctcg ggttcgctgt tgctcaaagc cgccgcccaa
    25381 gccggcagcc aaatcagcct ttacggtcag gaaaaagatg tggcaaccgc gtcccttgcc
    25441 cgtatgaata tgattttgca caacaacgaa accgccgaaa tcaacaccgg gaacaccttg
    25501 tccgattcgt ctttccgtga tgaaaacgac gggcttaaga ccttcgattt tgccgttgcc
    25561 aatccgcctt atcccgcccg aaaaaaacgg cgattacgcc tttttgctgc atctgctcaa
    25621 aagcctgaaa ccaagcggca aaggtgcgat tattcttccg cacggtgtgc tgtttcgcgg
    25681 caatgccgaa gcgcgtattc gcacggaatt gcttaacctt gaccttatta aaggcattat
    25741 cgggctgcct gccaacctgt tttacggcac gggcattcct gcctgcatca tcgtcatcga
    25801 caaagaacac gcccaaaccg cccaatttgc cgaagaggga acaaaccaag ttatcagtgg
    25861 cggcagcgtg tttatgattg acgcatcgcg cggctttatt aaagacggca acaaaaaccg
    25921 tctgcgcgaa caagacattc acaaaatcat cgacaccttc acaaaacagg aagaaattcc
    25981 ccgttacagc cgtatggtgc atttaagcga aatcgcagca caagattaca accttaatct
    26041 gcctcgctat atcgacagcg gagaagtcga agacctgtaa aatctcgccg cctatcttta
    26101 tggatggcgg catacctgcg cacgatatgg acgcattgaa agcctattgg caagttttag
    26161 gccgtatgaa aaacgagttg tttgccgaac acgatggccg ctttaccact atacaacgga
    26221 atcgattgca aatctttccc actgttcttg aaagaatgcg aatttcgatt taacttcggc
    26281 acaccgtctc aacagcttaa aatcctgcgg gattggtgtg gaatttaggg ctaatctagt
    26341 acagccccaa atttaatcca ctataaaatc gaaagcagcc aaatcaaagc ccatatattg
    26401 gcgcaccccg attacgccgc cttcaaagcc ggacacctgg caaagtttgc cgcgtggcac
    26461 actcaaaacg accttgccgt catccaaccg ggcagccggc cgggcaggct tatccggaaa
    26521 tggagcgaaa gcctgctgga cgcgttcaaa cccggcagcc tgattgaaga atacgatttc
    26581 taccaaatcc tgacggacta ctgggcggaa accctgcaag acgatgttta tctcatcgcc
    26641 caagatggct ggaaggcggt taaaaacctg gccgaaatca ccaaagaaag cgatgaagcc
    26701 gcgaacctga ccgtcgtctt tgaggaaacc gaaaccggca aaaaaggcaa agccaaaacc
    26761 aagcgcatca gcaaaaaata ccgcagcgaa gtcatcgccc ccgagctggt tgcccgccgc
    26821 tacttttcag acggcatcgc caagctggaa gaaaaacaaa gcgagctgga acgcctaagc
    26881 caagaattgg aaaaccacat agaagaacac ggcggcgaag agggtgcgct gaacgacgta
    26941 ttggatgcaa aaggcaaact ttccgccaaa cttctgaaaa ccgcattgga agaaagcggc
    27001 atagaagaag gcgaacgggc tgttttacaa accacccaaa cactgatgac gcaggaaaaa
    27061 gccgcgaaag acgcagtcaa aacccaaatc gaagccctga accttgccgt attcaaacaa
    27121 ttcggccgac tttccgaagc cgaaatcaag cagcttgccg ttcaagacaa atggcttgcc
    27181 gatttacaaa gccgaatcga aaatcgcttg gaaaacagta ttcagcagct tatcagccgc
    27241 ttgaacacgc tggaagaccg ctaccgcagc ccgatggccg agcttgcccg agaagtggaa
    27301 aagtggcaaa gcaaagtcaa tgcccacctt gaaaatatgg gttttggagg ctgaaatggc
    27361 ggcacagaca ggctataagg cgagcgggtt ttgagacctt tgcaaaattc cccaaaatcc
    27421 cctaaattcc caccaagaca tttaggggat cgcggttcgg gtgtccgcac cgcttaatac
    27481 gtcgtcgtcc acgaactgac ccatttgctc gaacgccatc gcaacgcccg ttttatggcg
    27541 catatggaca actttctccc aaactggcaa agcatcaaac aacagcttaa tgccttggag
    27601 ttatttgcac aaatatataa tttaacataa tatacattat gcgaactatc ggaaacagtt
    27661 gtacgtgtcc ctgtggtctt tccaagtagg aaaattaaag tatggccaat gcggctgaat
    27721 gtatagcccg gagcatccgc gtatccgaag gtactgaacg acatgccctg catccctttc
    27781 gatgcttcca tccagccggt attgggaaat acgttgacct tcaatataat atacaggcag
    27841 ccgacacaca ggatatgccg ctgctttttc atcatttctt ctgtcaaatc cttggaacgg
    27901 tcgatttgaa aacacggctt tacatacgtc ccaattctct ttgcagtgct tggatatttt
    27961 gctgcctgtc caatacattg ctttgtaatg catttatttc ttgatggttg atgttgccgc
    28021 cctttgccag acgtgcttgt gataacattt tttgggcttc agtcagggct ttgcgttcgt
    28081 tgctcaattc tgcttcgaga atggagcgtc tgctgttgtt tacaggtgct tgttgaggcg
    28141 ggggcgtatt ggattttgcc ggcttggata ctgttttgac cggggcttta tatttgacag
    28201 cctgtccgcc gtttgacggt gatggtgtcg gttcggatgt ttggggcggg atatagcgtt
    28261 cgctgctgta gttgccgatt gggggcaaat cggttgagag gcagcttttg gacggcttgg
    28321 tggtgtaaac ggtttctccg ttgattgtgc aggtgtagat tttggccgca ttcgcaccca
    28381 atgggcttga aatcagggaa aagttgatta ggattaaggg gagttttgat ttcataatgt
    28441 gcttgatgct cggataatta tttgattttt tggtattttt tgtaatgctt tcttggcgtt
    28501 ttacgggctt ttcttttcgg ttggttgctt ttgattttct ttcggggctt tggcagcctg
    28561 cttgttcttt ttcccggcag gttttttgtt tttcttttta ctttctatgt gtattttggc
    28621 ttcttaactg agttttttaa ttttcaggcg gtatccgcct ccctgatggc tgctgatttt
    28681 aggtaaatcc gcatcggcgc acgatcctgc tggggctgat gtatatacgc ttttgccatt
    28741 tgagttgcaa tggtatacgg aggcttgcgc cgccgcgccg gaaagggaca ggagacccaa
    28801 ggcggcaaaa agtctgatgt ttataccggt aaaaggcggt gataacgccc gaattatacc
    28861 gttattggca ggcaaagata agcaccctgc ccgcgcttct ttatcgctcg gcaaactgtt
    28921 tctcgatttc agtttcaatc cgttccaatt cttcttgaca agccagccaa acctcttcga
    28981 tttgggcaag ttgtgttttg acttttgcca gctgggataa ggtattttgc aatttttctt
    29041 tgtttgcttc aaggtaagcg tcttcttgtg ctaaaaatgc ttcacatgcc gtctgaattt
    29101 cggaaagctg cgccatttct ttttcggcac ggtctatttt ctgctgtatc ggcttgccgc
    29161 gtcgggcttt ttcctgacgg atttgcgctt cgatgcgctt ggtgtctttg cggctttggc
    29221 tttgtgcgga tgctgcgggt gcggcggcgg cgttttcctg tgccaaacgc cattggcggt
    29281 aatcgttcaa atcgccgtcg aagttcttca gacggccttt gtcgatcagg aggaagctgt
    29341 cggtagtcgc ttcaagcagg ctgcggtcgt gcgatacgat gattaaggcg ccttggaaac
    29401 tttgcagagc gagcgtcagg gcgtggcgca tatccaagtc cagatggttg gttggttcgt
    29461 caagcagcag caggttcggc ttttgccaga taatcatggc aagggcgaga cgggcttttt
    29521 ctccgccgga aaaaggttcg gttttctgca acgccatatc gccggcaaag ttgaagccgc
    29581 cgaggaagtt tcggatttct tgttcgcgta cttcgggaga aagctgctgg atatgccaaa
    29641 cagggttttg gtcggcgcgg atggtatcga gttggtgttg ggcaaaatag ccgatattga
    29701 gtttttcgga acggacgatg ctgccggaaa gcaaatcgat tttgcccgcc aaggctttga
    29761 taaaggtaga tttaccgctg ccgttgacac ccaatagccc gtagcgtgcg ccgctttcca
    29821 gcgacagggt aatgtcatgc agaacggttt tgccttcgta acccaaatca gcgtgttcta
    29881 gctttaacaa aggattgggc agatggtcgg gatggtaaaa ctcaaaggaa aactcgctgt
    29941 ccagatgcgc gggcgcaacg cgttctagct ttgccaaagc cttcatgcgg ctttgcgctt
    30001 gaacggcttt ggtggctttg gctttgaagc ggtcgataaa ggattgcaga tgtttgattt
    30061 gcgcctgctg tttgacatag gcagcttgtt gttgcgcgag acgctgcgcg cgttcgtttt
    30121 ggtaaaaatc gtaattgccg ccgtattgcg tgagtttttg ctgcgataat tcaatggttt
    30181 gggtagttgc cgcgttgaga aaatcgcggt cgtgggaaat gatgatttgc gtgcagggta
    30241 aagaagcaag atggttttcc agccacaaga cggtttccaa atccaagtgg ttggtcggtt
    30301 cgtccaaaag cagcaaatcg gcgcggcaaa tcagggcttg ggcgagattg aggcgcatac
    30361 gccagccgcc ggaaaaggat ttgacggggc ggctgtgttc ttcttgcgaa aaacccagcc
    30421 cgttcaacaa ttttgccgcg cgggccggcg cggtataagc gtcgatttct tccaatttgg
    30481 catgatattc cgcctgcttc atgccgtcat tttgcgcttc cgcctgcctc aatgccgtct
    30541 gaaaagcctg caactcggca tcgccctgca aaacgtaatc caaagcggaa atatccaaat
    30601 cgggcgtttc ttgggaaacg gaagcgagcc gccagttttt cggaatcgag acatcgccgc
    30661 cgtcctgagt gattttaccc ttgattaagg caaacaggct cgatttgccc gttccgtttt
    30721 tgccgatcaa accgacgcgc tgaccgggat tgacggtagc gttggctttg tcgagcagga
    30781 ctttcaaacc gcgttgcagg gtgaggtttt tgatttcaat cataacggaa acatcgtcgg
    30841 gcgggaaaag cccgtatttt acctgaaagt cagtgccgat gccgtctgaa acgggaaatt
    30901 tacggctgaa gccaagccca agccctgcgc ccttccgagt gcaggaaaac caatgtcctg
    30961 aatgccgaat cggtattcat acattccacg ccgattccga ttcgggaaaa atccgccatg
    31021 attttgggat ggataaactc ctgagccgcg cccgtcccga taatcaatat ttccggatag
    31081 tcaacaggtt tgatgtcgga caacaggttt tccggagtca gatcggacaa ggttcggcat
    31141 tgcgacaggc agaccgaatc cttatgtaca agcacgggtt tatggaaact ttgcctcgcc
    31201 agccggattc cgcccgcacc gcattcatat tccgcaaact gtccgtctat cggattttct
    31261 tcaaacaaca ttttttatcc cgttgccgca tcatctgctc cgcaaggggt gcaaaatcag
    31321 acaaattcat gtaggattgg cagatttcat ctgacccgcc tgccggtttc agacggcatt
    31381 tgattcaaag tgcggcacca ttatatcggc ggcagatatt ttcgtcttta tatattcaca
    31441 ttcaatcggc ttgcaaggag acacaatgaa gccagtaaac atcggtcttt taggtttggg
    31501 tacggtcggc ggcggtacgg ctgccgtgtt gcgggacaac gcggaggaaa tttcccgccg
    31561 cttggggcgc gaaatccgta tttctgccgt atgcgacttg agcgaagaaa aagcccgcca
    31621 aatctgcccg tccgcagcct ttgtcaaagc cccgttcgaa ctggtcgcgc gtgaagacgt
    31681 cgatgtcgtc gtcgaattgt tcggcggtac cggcattgcc aaagatgcgg tgttgaaagc
    31741 cattgaaaac ggcaaacaca tcgttaccgc caacaaaaaa ctgctcgccg aatacggcaa
    31801 cgaaatcttc tcgctggcgg aagaaaaaaa tgtcatggtg caatttgaag ccgcagtagc
    31861 gggcggtatc ccaatcatca aagccctgcg cgaaggtttg gcagcaaaca ggattaaatc
    31921 catcgccggc attattaacg gcaccagcaa cttcatcctc tccgaaatgc gcgaaaaagg
    31981 cagcgcgttt gccgatgtac tgaaagaagc gcaggcattg ggttatgccg aagccgatcc
    32041 gaccttcgac atcgaaggca acgatgcggg ccataaaatc accatcatga gcgcactggc
    32101 attcggcacg ccgatgaact tttccgcctg ctacctcgaa ggcatcagca aactcgacag
    32161 ccgcgacatc aaatacgccg aagaacttgg ctatcgcatc aaactgttgg gcattacccg
    32221 caaaaccggc aaaggcatcg agctgcgcgt ccaccctacc ctgattcccg aaagccgcct
    32281 cttggcaaac gtcaacggcg tgatgaacgc cgtgcgcgtc aacgccgata tggttggcga
    32341 aaccttatat tacggcgcgg gcgcgggcgc attgccgacc gcttccgccg tggttgccga
    32401 tatcatcgac atcgcccgcc tggttgaagc cgataccgcc caccgcgtac cgcatctggc
    32461 gttccaaccc gcgcaagtcc aagcgcaaac catcctgcct atggacgaaa ttaccagcag
    32521 ctactacctg cgcgtccaag ccaaagacga accgggcacg ctggggcaaa tcgccgcgct
    32581 gttggcacaa gaaaacgtgt ccatcgaagc actgattcaa aaaggcgtga ttgatcagac
    32641 cactgccgaa atcgtgattc tgacccacag cacggtcgaa aaacacatca agtcggcaat
    32701 cgcagccatc gaagcactgg attgcgtgga aaaaccgatt accatgatcc gcatggaaag
    32761 cctgcatgac tgagccgaaa cacgaaatgc cgacggaaga gcaggttgcc gcgcgcaaaa
    32821 aagcaaaagc caaaatccgc accatccgca tttgggcgtg ggtcattttg gcgttgctcg
    32881 ctttaaccgc cctgctctcc caatgcgcga tgtccaaacc gcaggcaaaa cagaaaattg
    32941 tcgagtcttg cgtgaagaat attccgtttg ccgaaaaatg gcaaaacgat ttgcgggccc
    33001 gcggtttaga ttcaaacaat acccgcctcg ccgtcgacta ctgcaaatgt atgtgggagc
    33061 agcctttgga cagattgagc gagaaacaga ttagatcctt cggcaaactc ggcgcacaag
    33121 aacagcttga cctgctcggc ggcgcaaatg cctttgaagc acgtgacaag cagtgtgttg
    33181 ccgatttgaa atcagaataa tgtggaccga taaaaaagcc gattctttaa agaatcggct
    33241 tttttcataa aaaacggctt acagtgcgtc tttcaatgct ttacctgcac ggaatttagg
    33301 cgttttggcg gcggcaatgg tcagaggctc gccggttttg gggttgcggc cttggcgttc
    33361 cgcacgttcg cccacgtaga aagtaccgaa accgaccaaa gtaacggtgt cgccttgttt
    33421 cagggcggtg gttactgcat tggtagtggc atccaaagct ttttgtgcgg cggctttgga
    33481 aatgtcggct tcttgagcaa tcgcttcgat caattcagac ttgttcacaa tcagtccctt
    33541 cctgtcttaa aaaatgatga aatgcccgaa tactcggggt ttgtactgct tgagcaactt
    33601 tcgctttata gcaattctga aattgccgtg tcaagcaaaa aatacggaat caccctattt
    33661 gacaggcttt caggacgaaa ccgcattttt acaacacatt tcctgcgttt caatgtttgg
    33721 ttgccctgct gcggggtttt ggttttgaag cggattccgc cgccgcttcc acaccagaag
    33781 gttctgccca aggctcaggc tggctttcca aacccagagc caatacctcg tctatccatt
    33841 tgaccggatg gatggtcagg ccggttttca cgttttcagg gatttcttcc aagtctttga
    33901 cgttgtcttt cggaatcagg acgtgtttga tgccgccgcg aagcgcagcc aacagttttt
    33961 ccttcagacc gccaatcggc aggacttcgc cgcgcagggt aatttcgccc gtcatcgcta
    34021 catcggcgcg taccgggatt ttggaaaatg cagacaccat cgccaaagtc atggcaatgc
    34081 ccgcactcgg accgtccttc ggcgttgcac cttcgggaac atggacatgg atgtcttttt
    34141 tctcgtaaaa atcaggagcc aagcccactg attccgcgcg ggagcggaca accgaccatg
    34201 ctgcggacac ggattccttc atcacatcgc ccaactggcc ggtgcactga atcacgccct
    34261 tacccggcaa tgctgcggct tcgacggtca gcagttcgcc gccgacttct gtccacgcca
    34321 gaccggttac ttgtccgata cggttttcgc tttcggcaac gccgtaatcg aagcggcgca
    34381 cacccaaata gtcgtgcaga tttttctcat ttactttaac cgctttaggt ttggctttgc
    34441 tggttttctt ggtttcagac aacctcttct tatcttcgtc caaggtaatc tgcatcacca
    34501 ccttgcggca gattttggca atctcgcggt cgagcgaacg cacgcccgcc tcgcgggtgt
    34561 agtaacggat aatatcgcgc accgcgcttt cttcgattgc caattcccct tctttcacgc
    34621 cgttgcgctt catttgcttc ggcacgaggt actgcatcgc gatattgatt ttttcgtctt
    34681 cggtgtagcc ggacagacgg atgatttcca tacggtcgag cagcggggtc gggatattca
    34741 ggctgtttga agtcgcaata aacatcacat cgctcaaatc gtaatccact tcggcataat
    34801 gatcggcgaa cttgttgttt tgctcgggat caagcacttc gagcaacgcg ctggcgggat
    34861 cgccccggaa gtcgctaccc aatttgtcga tttcgtcgag caggaacaat gggtttttta
    34921 cgccggcttt tgccatattc tgcagaatct taccaggcat agagccgata taggtgcggc
    34981 ggtgtccccg gatttcgctt tcgtcgcgca cgccgcccaa agccatgcgg acatatttcc
    35041 gccccgttgc tttggcgatg gattcgccca aagaggtttt gcccacgccc ggaggaccga
    35101 ccaggcacag aatcgggcct ttgagtttgt ccatacgttt ttggacggcg aggtattcca
    35161 aaatccgttc tttgactttt tccaggccgt agtggtcggc atccagcacc agtccggctt
    35221 tggcgatgtc tttgctgacg cgggattttt tcttccacgg cagttcgagc agggtgtcga
    35281 tgtagttgcg tacgacggtg gattcggcag acatcggcgg catcattttg agttttttca
    35341 gttcggacag gcatttttct tccgcttctt tactcatacc cgcctttttg atgcctgctt
    35401 ccaaggcatc cagttcgccg ttttcgtctt cttcgcccaa ttctttgtgt atcgctttaa
    35461 tctgttcgtt cagataatat tcgcgctggg atttttccat ttggcgtttg acgcgtccgc
    35521 gtatgcgttt ttcggcctgc ataatgtcga gttcggattc cagctgtgcc agcaggaatt
    35581 ccatccgttt gccgatttcg gaaatctcca aaatctgttg gcgttgcgtc agtttcaact
    35641 gcaaatgcgc tgcgactgta tcggttagcc ggctgttttc ggcaatgccg ttaatgctgc
    35701 cgataatttc ggcggggatt tttttattga gtttggcgta ttgttcaaac tgcgccaaca
    35761 gggtgcggcg cacggcttcg aggtcggtat tgccgcccgt gtcttcttcc acgaccgcct
    35821 ctatatggga aacgaacagc ccgcccgtgt cttcaatggt cagaacacgt ccgcgataca
    35881 gcccttcgac caatactttt accgtgccgt cgggtagttt caacacttgc aggacttgtg
    35941 cgaccgtacc ggtctgatac aggtcggcgg caaccggttc ttctaccgcc gcatcggttt
    36001 gcgccaacag gaaaaccggc tcctcgcggg taatggcgtt ttccagtgcg gcgatggatt
    36061 tcggcctgcc gacaaacagc ggcagaacca tatgcgggta aacgacgaca tcccgcaaag
    36121 gaagggttgc caaggcggca tattcctcaa aatgcttttc tttttgtgtc ataggtactc
    36181 tcttgtgtct gacagattgc cgattttcgc gtacattggg gttgaaggta ttatttcaag
    36241 catatgtggt ttatttatgg agtttgatgc gatgccgtct gaaacattcc ggcttcagac
    36301 ggcatgggct tggaaagaca aggcgggaac aaaaaaccgt tctgtgttgc cgctccttgc
    36361 tgtaccatcc gtatggtttg cggttctgcc gccctatttt caaaacatga cgcaggtatt
    36421 ccatgtcttc ttttattttt cccgataacg ggttcgaatg gcgcaacgaa agtctgcaaa
    36481 agccgcccaa aggttggcat tatgtccgcg aaagcggtgc aaacggcatt ttgaaggctg
    36541 cctatcaaaa tattgcaact gtctggcagg gcgatttcca caatgccaaa caggtgcttt
    36601 ccgcaatgaa gaagaaggtc cgcaaacctg ccgccgtccg ttccgatgcg gatatcgccg
    36661 cccttttcca cgcccaccgt atgaagcagg cgcagcagag ccgtattctg aatatgcttg
    36721 ccgttgaaat ccgccccggt tttgtgttgg acaacaaacg cgcgcccgat atacgcgccg
    36781 ccctgctcga cgtgtacgga gaggcggacg gcaaaccgtt tttcctgccg ctcaatctgc
    36841 tgctggggtt tatgggtgcg cacgagtggc ataagaaagg ggttgccgtt ccgcagctgg
    36901 gcggcagcat acacgttcct ttcggcgtat tctcgccgtt gcgcggcgaa tacctcgatc
    36961 tgctcgccca tgcgccgtca acgggttttc agacggcatt cgatatcggg acgggctcgg
    37021 gcgtgcttgc cgccattttg gcgaaacagg gcattccttc cgtcatcggc acggatacca
    37081 atccgaaagc cgtcgcctgc gcccgtgcca atattgcccg tttgggcttt gaaaaacagg
    37141 ttgaaatccg cgaaaccgat ctgtttcccg aagggtttgc cgatttaatc gtctgcaatc
    37201 cgccctggct tcccgccaag ccgacttccg ccgtcgaatc cgcgttatac gaccccgaat
    37261 ctgcgatgct ggctgcgttt ttgcgggatg cgccgaaaca tctgaatctc gacggagaaa
    37321 tccgcctgat catttctgat cttgccgaac atctgcacct gcgtccgtcc gattttctgg
    37381 ataaggcatt tgctcaggcg ggtttgcgcg ttgcggatgt tttgaaaacc aaaccggttc
    37441 acaaaaaagc tgccgatccg aacaatccgc tggcttttgc ccgaaacagg gaaactactt
    37501 tcctgtaccg tttgaaaaag gcataagggg cggcggtgcg cttccggcgg caggcatcgg
    37561 aacgccagcg ccgccggccg gattatcctt gtgaaaatac ccgctcaagc atactgccca
    37621 acgccgtctg acgcgttttg acggtggcgg cagcttgccg gaaggcttct tccccgccga
    37681 agaatacgaa cttctctctc gcgcgggtaa tggcggtata taacagctcc ttactcaatc
    37741 cggacaatgc atcgtcccct tcgtccgaag gtgcggcgga aggcggcagc agccagactt
    37801 cccgatattc cgaaccttgg cttttgtgga cggtcatggc gaatgcgggt tcaaattcgg
    37861 gcaggcagct taccgctacc tttttaaatc cgtccgcatc ggcaaaatag gcggcaaggc
    37921 tgccctgccg tccgacatct tccataatca gtccgatgtc gccgttgaac agttcaagcg
    37981 cgtagtcgtt ctgtctgatc ataatcggtt ctccggcgaa ataggcctga tgttccggta
    38041 tgttcatttt gcggcgtaca tggcggcaat aggcttcgtt gaagtcttcc gaatcctgcc
    38101 gccaagctgc cagaaccacg atatccgaga tgcccacgta tgcggcttcg atattgccgt
    38161 cttttaccgc ctgccaatag gctttgtgtg cccggtacaa cctttcgact tgagcgttcg
    38221 gactgcattc cgaatgttcc agttcgtccg gaaaccggtc aaacaatgcc cacgccccct
    38281 catcgcccga tacggcggca cgggcaaggc agccgatgcc gctgttgtcg ccgaagcggt
    38341 ggctgaacga cagatgggcg gtgttttgcg ccagcacggg agcatcttcg tgtaccggaa
    38401 atccgtgttc gggcaggtag aggttcaaac ttttatacgt ttctctgtcc aaaatggttt
    38461 tttgcgacaa aacggacagc accgccccga ttccgacgga cgggagctgg ttttcatccc
    38521 ccagcagaat cacgcgcgcg ccggttttga ccgctttcaa aagttgcagc atcaatgcgg
    38581 tatccaacat ggaggcttca tcgacaatca gcacatcgaa cggcagcgga cggatatggt
    38641 cgaacgccgc ctgcattttg ggcgggcgca gcttcagcaa tcggtggacg gtttgccctt
    38701 ccagtttgag caaatggcgg cggacggcct ccggcgcgtc aaaaccgttg attgcacggt
    38761 gcagcgcgcg cgccatatgt gccgccgctt tgcctgtcgg tgcggcaagc gcgatgttgg
    38821 gaagattttc gtcttcaccg caaatcagcg ccagcagttt ggcaaccgtt gtcgttttgc
    38881 ccgttcctgg cccgccggta atcaccataa aagactgcaa cagtgccaag gcggcggcat
    38941 cgcgctgccc ttcgctgccc gtgccttgaa accattttgc gaggttttgc ctcgcgcctg
    39001 ccgcgtcggg ggcggatgtg ccggctgccg ccaagcgttt tatctcggca gccaaatcgt
    39061 attccaactg ccacatcctg cccaaaaaca gccttctgcc ttccaaaatc aaaggcgcgg
    39121 cggatgttcc gacaacgggt gcgagtgccg acagcgcgtc agcctcgcca ccgctcaaac
    39181 ggataaacga atgaccgttt tgcaatgcct gaaacaggcg ttcggtgcag tttgcaagca
    39241 cttcgtcgcc ggaacccgca tagtgttcca aaaaacggat tgccgccctt gccgccgctt
    39301 gggcaaattc atctgtctgc cgttccattt tattccttat ccaaatgccg tctgaaggcg
    39361 tggggcttca gacggcgcgg tgttgtttcg gctgtttagg cgtttgcgcc ctgtttgggg
    39421 tgcaggctgc cgtctttcat gaccatcacg cgctcgaagc ggccggcgag ttcgtcgtcg
    39481 tgcgttacga ccaccagccc cgttcccaat tctgttttca gttccagcat catatccaaa
    39541 acattcctgg cgttcgcacg atcgagattg ccggtcggtt cgtcggcaag caggcatttg
    39601 ggttgggtaa ccaaggcgcg tgcaatggcg gcacgctgcc gctcaccgcc tgaaagttcg
    39661 cccgcgcggt gcgtcgaacg gtgtttcagt ccgacctttt ccagcatcgc cattgccgcc
    39721 tcctcagcct cttcacggct ttttttgccg atcagaagcg gcatcatcac attttccagt
    39781 gccgaaaatt caggcagaag atggtggaac tggtacacga aaccgagatg gcggttacgt
    39841 aaatcgccca aacgccgctg gtttaaggta cgcaaatcct cgcccatcag cagcaccctg
    39901 ccttcagacg gcatatccag cccgcccaaa atatgcagca gcgtcgattt gccgctgccc
    39961 gaagaaccga tgatgccggt gctttcccct gcgtggattt ccaaatccaa gccgtgcagc
    40021 acccgaacgt ccaaaccgcc gtcccgatag cgtttgccca cgccttcgca tttcaaaatc
    40081 aactcactca taacgcaaag cctccgccgg ttgggttttt gacgcgcgcc ggctcgggta
    40141 gagcgtggca acgaaagaca gtcccaaaga aatgcaggca atcagggcaa cgtcgcccat
    40201 atcgacatcg ctgggcaggt agtcgataaa ataaacctgc gaattgatga ggtggacacc
    40261 gagcaggttt tcaaaaaacg ccacgaccct gccgacgttc caacccaaaa gcacgccgca
    40321 gaccacaccc gccagcgtgc cgaaaaagcc tgaaaacgcg ccctgcacca taaaaatctt
    40381 catcacgcca gcaggggaaa gacccaaagt ccgcaaaatc gcaatgtccg cctgcttttc
    40441 cgtaaccgcc atcaccaggg aagagacaag gttgaacgcc gccacagcga taatcagcgt
    40501 caggatgatg aacatcatcc gtttttccag ttcgaccgct tcaaaatagc tgcggttgct
    40561 gtacgtccaa tcgcgcaccc aaaccgcgtc cctttgcgcc tccggaatca gtgttgccgt
    40621 caaggcggga gcgttttgcg gatcggcgag cttcagccgc agccccgcaa cttccttatc
    40681 caaacggtac agcacgcgcg cgtcttggat atgcgtcatt gccaatgagt tgtccacttc
    40741 gtaaacgccc gtcttaacca gaccgaccac ggtaaactgt ttcaacctcg gtacgactcc
    40801 ggcgggcgta acattgccct ccggcgtgat gacggtaact ttattgccga cttccgcccc
    40861 caaagcctcc gccaagccga caccgaggat aatgtcaaac tcgcccggaa tcagatcttc
    40921 aaatttgcct gccggcattt tgtcgccgta ttccaccact ttgcgttctt cagacggcaa
    40981 aatgccgcgc atctgcacgc ccctgatttc gcccgcattg gccagcaatg cctgattgga
    41041 aacatagggc gcggcagcca aaataccttt gcggttttcg gtaaaccgaa gcaggttgcg
    41101 ccaatccgta tccgtattat cgatatagcc gatttcggcg tgcggcgcga cattcaggag
    41161 ctgcccgcgt atttctttct gaaagccgtt cataaccgac aagacgacaa tcagcgcggt
    41221 tacgcccaag gcgattccgg caatcgaaac catcgtgata aacgacataa agccgttgcg
    41281 ctttttcgcc ctgagatacc tcaagcctat ccaagcctct agagaaaaca taacgctacc
    41341 ttaaaaatgt ctgcaaacgt gccgccccgg acggcggttt gggggcggcg gcaaaagttt
    41401 attgtaccgt aaaacccagg cagcgtccga acgcccgagt gcgggggacg gggcggcaga
    41461 gcgggcggaa aacttgcaca acgcgtcaaa ctgccctatc ctttccttca gaaaaaccgt
    41521 ttcttgagga aaacaatgaa tatccgaact gcttttgctt tgtgcgccat cgccttatcc
    41581 gccgctgcgg ctgcctacgc caaagaaatc aaaatcgatg ccaacaacac gccttattcc
    41641 gaagccgacg cgcaaaagct ggcggcaacg gcagtcggta tgggtgttaa ggaacctatc
    41701 agcctgaacg gcggcagcgg cagcattacc gtgtccggca gcagcgcgac gcagtgcgtg
    41761 ttcaaagtcg gcaacggagg cgcattgcag attcaagggc taaactgcaa gtaaaccgcc
    41821 cggaaaaatg ccgtctgaag gcttcagacg gcattttgca ttggcggcgt tatgccccgc
    41881 cttctttaat caggcggcgt tcgtacaccg cctgcgccag cgttcccgca tcgacatatt
    41941 ccaattcgcc gcccaaggga atgccctgcg acagcctgct gactttgtaa ggcaggtttt
    42001 taaaaaactc ggacaggaca tacgccgtcg cattgccttc tgcggtaaaa gcggttgcaa
    42061 taatgatttc ttcgacttcc ccgccgccca gccgttgcgc cagcctgtcc aatgcgatgg
    42121 cggatacgtc cattcccaat gccgtattga tttgccccat caggacgaaa tacagcccgt
    42181 cgtggcagtt tgccgcttcc atattcgaca cgtcggcagg catatgcacc accatcagcc
    42241 gccgcccgtc gcgtgtttca tcggcacaaa tatcgcacaa tccgccttcg caaaacgtgt
    42301 tgcacatcgc gcaatggtaa acctgcttca atgccgtctg caaggcatcc accagttttt
    42361 cagcctcttt gcgcttgtgt tggagcaaat gatacgctat ccgctgtgcc gatttcggcc
    42421 cgacgttggg taaaaccttc agcgcgtcga tcaatccttg gaaggcatct tgttttttgt
    42481 ggctcatcat attccgccgt atgggaaaac ggccggaata ttccgaccgt tattttgtca
    42541 acaaaagtgt caattactga ccgtcgccgt tgtcgaccga ttgcgctcct ttggtctgtt
    42601 tgattttgcc gttgaaataa cgtatcaaca agtcgaaagt attggcagac tgctgttgcg
    42661 ccaaagcctg ttttgcaagc ggaagctgtg cggcgatatc atccggcggg gttacagcct
    42721 gtacttcgac aatcacgggt gccggcagac cgatcagcct gacgtaggcg ggtttgccgt
    42781 ttgccggttt tgctttcagc agttccgcat aagcctcggg cggcatggac tgccttgcct
    42841 gctgtgcgcc caaaacggac acttccgacc atttcacgtc aacagccttg ccgccgttca
    42901 gttgggtaag cacgtctttt gccttgtttt cggcaagttt ggcggcttcg gtacggatat
    42961 aagcctgacg taccgcgtct ttggcttcgg caaacggcag ggttttctct tcgcggactt
    43021 ctttggcgcg gacgacccac gcggtttcgc tgttgatggt cagcacttcg gaattgtgtt
    43081 ttttcttcaa tacgtcgtcg ctgaatacgg cattgatcag gttttcgggc ataccggaca
    43141 tttgcgcgtc ctgcctactc agccaagttt cttgggtttc gactttcaaa ccgctgtttt
    43201 tggcggcttc ggcaagcgag gaaggatggt tgaacgcatc gtcgcccaat ttttcttttg
    43261 ccttgttgaa gtcggcaacc gcctttttca ttttcaattc gttttcgacg gcggcttttt
    43321 cctgctcgaa agaaggtttg gcttcatttg ccggcaaacg cgccacgcgc tcttcaaatg
    43381 catttttcac ttccgtttca ctgacggtct gcttgtctgc aaaatccttc agattcaagg
    43441 cgacatattc caatttgacc gcctgcggca gcagatagtc ttttttgttc gcattataaa
    43501 atttctgcaa atcggcttca gacactttga cttgggcgat gaactcgtcg gggttgaaag
    43561 tgtgcgaacg gatggtgcgg ttgacctgtg tcagcctgat cagctgttcc gcctgcgcgt
    43621 cgccgaccaa tacgccgttt tggacgaggt ttaccaaatt ctgcaaggca aactgatcgc
    43681 ggatttcttc gacaaactgg tcttcagaca tatggcgttg ggaaaggtag cggtttaaaa
    43741 gcgcgtggtc gaatttgccg tttgcgtcgt ggaaattggg atcgtccacg ataatttgct
    43801 tgatttgttc ggaagaaacc gaaatgccca tcagcttcgc gccctgtttc aggtaggcgc
    43861 gttgcagcag ggattggaac accgcgtcgc gcgaagggcc gccgccgccc gcctgttcgt
    43921 tttggacggc ggcgttaagc gactgctcgc tgattttttc gtcgcccact tggacgatgt
    43981 agtcgacacc cggatgggat accgtgctga ccccgaagcc gacgaaggtc aatgcaatca
    44041 ggcctaataa gacttgggcg ggcgttctgt atttttcgat ggaatggaac atattttaaa
    44101 tcgggatata gaatgggaac gggaaattca agtcgggtat tgtaacggtt tttatccctg
    44161 tctgcacggg gcttgccggt tgaagatgcc gtcgtaggtt tcttcgctga agccggcgta
    44221 aaccttgccg ccgcactcca atacgggacg cttgatcagg ctcggcattt cggacatcag
    44281 tttgacggcc tccactgtcg agaacagggc tttttgctgt gtttcggcat cgagtttgcg
    44341 ccagcttgtc ccgcgtttgt tgagcagggt tgccaaaggc acttgttcca gccacgagca
    44401 gatttccgct tcggacggcc tctgtttttt aaaatcccga aattcaaact ccaagccgta
    44461 tccggcaagc ctgtttttgg cttttttgac tgtgtcgcaa tttggaatgc cgtgaaggat
    44521 tatcatttgg aaaccttttg tctgaaataa taaaacggat attttactat aagtgtctga
    44581 aaatttgccc gtctgtttca gacggcgggg cggttatgtt acaatccgaa aattcgaaaa
    44641 atttaatctc ttgttcaata aaggctttac caatcatgat ttctaccaac ggcatcacca
    44701 tgcagttcgg cgcgaagccg ctgtttgaaa atgtatccgt taaattcggc gaaggcaacc
    44761 gctacggttt gatcggcgcg aacggctcag gcaaatccac cttcatgaaa atcctcggcg
    44821 gcgatttgga acagacgacc ggcgaagtgg cgattgaaaa cggcgtgcgt ttgggtaaat
    44881 tgcgccaaga ccagtttgcc tacgaagaca tgcgcgtgct ggacgtggtg atgatggggc
    44941 ataccgaaat gtgggcggcg atgaccgagc gtgatgcgat ttacgccaat cccgaagcca
    45001 ccgaagacga ctacatgaaa gccgccgaac tggaagccaa gttcgccgaa tacgacggct
    45061 acaccgccga agcgcgcgcc gccgagttgt tgagcggcgt aggcatttcc gaagatttgc
    45121 acaatgcgac catggcagaa gtcgccccgg gcttcaaact gcgcgtattg ctggcgcaag
    45181 ccctgttctc caaaccggat gtattgctct tggacgaacc gaccaacaac ttggacatca
    45241 ataccatccg ctggttggaa ggcgtgttga accaatacga ctccacgatg attatcatct
    45301 cgcacgaccg ccacttcttg aacgaagtct gcacgcatat ggcagatttg gattacacca
    45361 ccatcaccat ctatccgggc aactacgacg actatatgct cgcctccgcc caatcgcgcg
    45421 aacgcgccct gaaagacaat gccaaggcga aggaaaaatt gcaggaattg caagaatttg
    45481 tggcgcggtt cagtgccaac aaatccaaag cccgtcaggc aaccagccgt ctgaaacagg
    45541 cagacaaaat caaatcggag atggtcgagg tcaaaccctc tacccgtcaa aacccgtata
    45601 tccgttttga agccgatgaa aaagccaagc tgcaccgtca ggctgtggaa gttgaaaaac
    45661 tggcgaaacg ctttgaaacc cagttgttta aaaacctgaa cttcatcctt gaagcgggac
    45721 aacgcctcgc catcatcggc ccgaacggcg cgggcaaatc caccctgctg aaactcttgg
    45781 ccggcgcgta caaccccgaa tattcagacg gtctgctgcc ggacgaaggc agcatcaaat
    45841 gggcggaaaa agccagcgtc ggctactatc cgcaagatca cgaaaacgac ttcgatgtcg
    45901 atatggattt gagcgaatgg atgcgccaat gggggcagga aggcgacgac gaacaagtca
    45961 tccgaggcac tttggggcgt ttgctcttcg gcagtaacga tgtcgtgaaa aaagtgaagg
    46021 ttctctccgg cggcgaaaaa ggccgtatgc tttacggcaa actgttgctg ttgaaaccca
    46081 atgtcttagt catggacgaa ccgaccaacc acatggatat ggaaagcatc gaatccttga
    46141 acatggcgct ggaaaaatac aacggcacgc tgattttcgt ctcccacgac cgtcagttcg
    46201 tttcttccct ggcaacccaa atcatcgaac tggacggcaa aggcggatat gaacactact
    46261 tgggcgatta cgaaagttac ttggagaaaa aaggcgtagc ataaccgccg gttggaacaa
    46321 tgccgtctga agccgcttca gacggcattg ttgataactt taaaatagga agcatatgca
    46381 gacttacctc gtcggcggcg ccgtccgcga ttatcttttg ggcttgcccg tcaaagaccg
    46441 cgattgggtg gtcgtcggcg cagacgcaca aaccatgctg gcgcaaggct tccagccggt
    46501 cggcaaagat tttcccgtgt ttctccatcc cgaaacgcac gaagaatacg ccctcgcccg
    46561 caccgagcgc aaaaccgcca aaggttacgt cggtttcagt ttccacaccg acaaagacgt
    46621 tacgctggag caggatttga tgcgccgcga cctgaccatc aacgcgatgg cgcaagatgc
    46681 ggacggcaag attattgacc ctttcggcgg acaacgggat ttggcggcag gcattttgcg
    46741 ccacgtttcc ccggccttcg ccgaagaccc cgtccgcatc ctgcgtactg cccgctttgc
    46801 cgcgcgttac aggtttgaaa tcgccgaaga aaccataaag ctgatgcggc agatggtggc
    46861 aaacggcgaa gcggacgcat tggttgccga acgcgtctgg caggagttgg cgaaaggttt
    46921 gatggaaaaa aatccgcgca aaatgattga agtgttgcgc gaatgcggtg cgctcaaagt
    46981 cttgctgccc gaagtcaatg ccctcttcgg cgtgccgcaa cgcgccgact accatcccga
    47041 aatcgacagc ggcatccata ccctgatgac gctgcaacgc gccgccgata tgggtttgag
    47101 cctgcccgaa cgctatgccg ccctgctgca cgacttgggc aaagccaaaa caccgtccga
    47161 catcctgccg cgccaccacg gacacgacct cgccggtgtc gaacccgtgc gcgaagtcaa
    47221 tcagcggctg cgtgcgccga aacattgcgc cgagcttgcc gaattggttt gccgttggca
    47281 cattattttc caccaagtcg gacagcttaa aagccaaacc attctgaacg tattgaaaaa
    47341 aaccgatgcc ttccgccgtc ccgaacgctt tcagacggca ttgaacgtct gcattgccga
    47401 cacgcaaggc cgtctgaacc gcgaacacac gccctacccg caacgcgcgc actggctcgc
    47461 cttactcgaa gccgccaatc aggcggattc gggcaaaatc gccgccgaat gccgcgcgca
    47521 gggaaaagcg caccttatcg ccgaacaaat cgaccgggcg cggctggcac aaatcgcccc
    47581 attgcaaaaa gcgtttcgag cggcgcaaga caaaacagaa aaacattaaa acgtccaatg
    47641 cagccacttt tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta
    47701 cagatagtac ggcaaggcga ggcaacgctg tactggtttt tgttaatcca ctataaagtt
    47761 ttgaggacga tacccaatcc aagctttgca acagccgccg ccatatccgc tataattcac
    47821 gcttcagcca ttccgccccc gacataaaat catgaccctg aaaaccgatt tattgcctaa
    47881 aatcaacaac gaagattatc aacgcctcat cctcaaacac agtgcggaat ttagcggtgg
    47941 cgaaatccgc ctgttgaacg aaatcctcga aaaattcaat ttcgacgttg ttcaggcgca
    48001 ggcattggcg caagccgtca tgcagcaaat ccgcttcgac cccaacgcct accacatcga
    48061 cagcgacgac gaagacacca ccggcatctg cccccactgc atcaacccgc ctatgccgcc
    48121 cctgcgcgac tatctcgttt ggcgcgaaac ccgtggataa aacgcttttg accgttatct
    48181 tttcaatgcc gtctgaaacg ccgccgaccg ttcggacggc atacccgaca aagggaacac
    48241 tatgctgcaa accgacaacc tgaccgccgc gcaaccgcaa cgcatcgttg ccgcccaaac
    48301 cgcctccgca caggaagaac tgctcgaacg cgccctccgc cccaaaacgc tggacgacta
    48361 catcgggcaa gacaaagcca aagaacagct tgccattttc atccaagccg ccaaaaaacg
    48421 cggcgaagca ctcgaccacg ttttgctctt cggcccgccc ggactgggca aaaccacact
    48481 ggcgcacatc atcgccaaag aattgggcgt aaatttgcgc caaaccagcg gccccgtcct
    48541 cgaacgcgca ggcgacctcg ccgccctttt gaccaacctt gatccgcacg acgtattgtt
    48601 tatcgacgaa atccaccgcc tcagccctgt tgtcgaagaa atcctctatc ccgcgctcga
    48661 agactaccgg ctcgacatta tgataggcga agggcccgcc gcccgttccg ttaaaatcga
    48721 cctgccgccc ttcacgcttg tcggcgcgac cacccgcgcc ggtatgctga ccaatccgtt
    48781 gcgcgaccgc ttcggcatcg tctcccgcct cgagttttac gaaaaccgcg accttgccac
    48841 catcgtcagc cgttcggcac aactcctgca actcgatatg tccgaagaag gcgcggaaga
    48901 aatcgccaaa cgcagccgcg gtacgccgcg catcgccaac cgcctgttgc gccgcgtgcg
    48961 cgatttcgcc gacgtgaaaa acaacggcac aatcgacggc ggcatcgccg atgccgcttt
    49021 aagtatgctg gacgtggacg tgcaggggct ggacgtgatg gacaggaaat tcctcgaagc
    49081 cgttttgcac aaattcggcg gcggcccggt cggtttggac aatgttgccg ccgccatcgg
    49141 cgaatcaaca gacaccatcg aagacgttat cgaaccctac cttatccaac aaggcttcct
    49201 gcaacgcacc ccgcgcggca ggatggcgac cgaacacgcc tacctgcatt tcgggctgcc
    49261 cgtcgaaaaa taacgcaatg ccgtctgaaa cagagctaat tttcagacgg catttctatt
    49321 tcaatcattg gcgcaaggtt cagcctgccg cttttttcca gttccgccct catcgcatca
    49381 atcaccgcct tatagtctgg tttgccgaaa atcgcagaac cggcaacaaa ggtatccgca
    49441 ccagctcggg caacggcggc aatattgtcg gttttgatgc cgccgtccac ttcgatggcg
    49501 atgtgccgcc cgctttgtgc ttcgtaccga tccagcatcg cccgcacccg gcggattttt
    49561 tcaagggtgt gcgggatgaa gctttgtccg ccaaatccgg ggttgaccga catcagcaaa
    49621 accatatcca gcctgtccaa tacgttttcc aacagatata cgggcgttgc cggattcaac
    49681 accagccccg cctgacagcc catatcacga atcaggctca agctgcggtc gatatggcgg
    49741 ctcgcttcgg gatgaaaagt gataatcgat gctcctgctt tggcaaacga ctgaatcagg
    49801 tcgtcaacgg gttcgaccat caaatgcaca tcgatcggca cgcttgcata aggcttcaac
    49861 gccgcgcaaa ccatagggcc gaaggtcagg ttcggcacat aatggttgtc catcacgtca
    49921 aaatggatca gatctgcacc tgccgcaatg acgctttcca cctcttctcc gaggcgggca
    49981 aagtctgccg acaaaatact gggtgcgata cggtaggcgg tcatattttt tccttcaatg
    50041 cctttacggg cggactcaac gcgggaaagc cccgaaacag cattattaac aaaagttaac
    50101 tgccataata ccatctttca cacggcaatc aagtatattg tccgaaatat ctggattaaa
    50161 ggaaaagccg tgccactgcc cgctccctgc cgtttcgcca aacctgccgc ctctttttta
    50221 agtatggctt tgctttcctg ccagctttcc cacgccgcca cggcttatat ccccctgaac
    50281 gattttcaac cgaactgcga catacgccga ctcggactga cacagggtca gcacaatgaa
    50341 ctgcgtaaaa tccgcgccgc cttcaaaatg gcgggcgaca gggcgcgttt gaaggttatg
    50401 cattccgaac acagccgccg ccggtctgtc gtcgaaatca tttcttcgga tgtttttaat
    50461 cggaacgagg cgcgcgatta tgtcgaaagc cgctatttgt ccggtatgga ttttgcggtg
    50521 gacgaattgg aaatccaaca ccgcttcttc catatcctca caccgcaaca gcagcaaatg
    50581 tggctttctt cctgcctcaa ataatccccg aaacgctcac ggcgcacgtt gctccggcag
    50641 cctgcccgtc cagtcgcagg caaactgcca agcggaacgt cccgagcggt tgccccgcat
    50701 ctgcgcccac tgcaacgccg ccgtccgagc tgcttcgtca tatggcacat caaaatcttc
    50761 caaccagctt tgcactgccg acagataatc gttttgatcg aacggataaa agctgagcca
    50821 caatccgaat cggtcggaca gggatatttt ttcttccacc gcttctttct gatggatttc
    50881 cccccgcatc cccgtcgtac cggcattctc gtcaaaatat tcgggcatca ggtgccgtct
    50941 gttggaagtc gcgtaaacca aaacgttggc gcaacgttga gacagaccgc cgtctaacgc
    51001 ggttttcaat gccttatagg tttcatcgcc gctttcaaac gacaaatcgt cgcaaaatac
    51061 gataaatttt tccggacgtt ccttcaaaag cgtcaacagg taaggcaggc cgattaaatc
    51121 gcttttatcg acttcgatca ggcgcaatcc cttatccgca tattcgtgta gcagggcttt
    51181 gaccagcgag gatttgcctg ttccgcgcgc gccgctcatc aatacattgt tcgcgggtct
    51241 gccgacaatg aactgttcgg tattacgcac cagcaattcg gtttgcctgc cgactcccgc
    51301 cagccttacc aagggaaagg tgtgcggatc gggcaagtgt tccaaaaaac cttttttgcc
    51361 cgcactctgc cagcggaagg caagcgcgtt ccaatccgta tgcccgggtt cgggcggaag
    51421 cacggcatcc aaacgccgca aaacggcata ggctttatcg agaaattcgt tcaattccat
    51481 ctctgcctca ctttgcatat ctttgcgcca tcagccgttc gacggtatcg acgattgcct
    51541 gcgtattcgg atcgatttcg atgttgatcc tgtcgccgac ctttctgctg ccgaacagcg
    51601 tccgttccaa agtttcggga atcagatgga cattgaaacg gccgtcttcg actttgccta
    51661 tggtcaggct gcaaccgtcc aagccgacga accctttggt caggatatag ggtttgagtt
    51721 catgcgggag cgaaaaccaa accgtgcggt tgaacccgtc ccgttcgatt tcgacaatag
    51781 gcacggttgc cataatgtgt ccgctcatga cgtgtccgcc gatttcgtct ccgaaacgcg
    51841 ccgcccgttc gatgttgacg caatcgcctt ccttcagcag ccccaaattg gtttttgcca
    51901 aagtttccgc cattaaatcg aaactgacgc ggtttccttc gatttcggta atcgtcaggc
    51961 agcagccgtt attggcgacc gatgcgccgc gttgcagatt gtccgccgcc tcttgcggaa
    52021 gctcgacgac ataagtgtga aatgcctccg acgggcggtg gattgccgtc agttttccca
    52081 atccttgaac aatgcctgta aacataatcc tgtttccctg tgtcggtaaa aatggtgcaa
    52141 attgtagcat ctccccgcga aaaatgccgt ctgaaatgcc ttcagacagc attatgcctc
    52201 cgattcgggc aaaaaccgcc cggtatggct tgacctttcc tttccacgcc ggtcggcggt
    52261 cttgccctta tccctcctgc aaatcgattt gcgtgttcaa gtcggcaaaa tgcccgtcaa
    52321 actcgaatct gaccggccgc gccctttgct gctgcaacca gcttcttaat gttttcatac
    52381 ccgaaaacag atagggaatc gcgctttgca gaatctgcgg gcggatatac ataatgttgt
    52441 agtgcatcgt tatcggcgtt tccacataat acgcattgca caacggtgtg cgtttcgaca
    52501 cggtttcaaa cctcgccacc aaatcgtccg gcagatacgg catatcgcac ggcacaacca
    52561 aaagccagtc agcagccgcc aactgcaaat cgttggctgc ggtacacaat gccgaaagcg
    52621 ggccgaaatg ctgccactgc cgcgcatcgg gaaaaatatg cggacttctt cgagcatatt
    52681 cttccaaatt ccggttggtg ctgatggcga tatggctgac ctgcggcctg accctgtcga
    52741 tgacatggtc tatcagtgcc ttacccccaa aagagcaagc cctttgtcct cgcctcccat
    52801 acggctcgcc tgaccgccgg ccagtatcag ggcaaaagtt ttcattgcgg atgttctctt
    52861 ggaaaagttc gaggttttca tgattgcagt ctgccgttcc caatgactca aaatgccgtc
    52921 tgaagcagac ggcaaataaa ttcatattat ctgaatttta tcataacatg atttaacact
    52981 gaagcggtgc ggattaagtt ttccttaacc aattctttct gagcagttgt atctaattcc
    53041 aaagaatgat attgtttgca ttatttggaa caatttttcg ccgagcatga tactgccagc
    53101 ccgtttttca gacggcatca gcctttccct gcgcctgaaa ctcctgaccg gactgtgggt
    53161 cgggttggcg gcattgtctg tcgttttgac actgctgctc tctttgcgtc tggaaaacgc
    53221 ggcctccgtc atcgaagagg cgggcaactt gagaatgcag gcataccgtc tggcatacat
    53281 ggcgggtgaa ggctcgcccc gtgcgcaaat tgacaatcag gttgccgaat ttgaaaaaag
    53341 tttaaaacgc attgcccaaa gcgatgccat ccatccgctg attccttcgg acacccctct
    53401 tgcttatgat ttgatacaat ccatgctgat tatagattgg caggcacaca tcctcccccc
    53461 gctccagtcc taccggcgac cgactcaggt cgatctctac cgctttgccg gaaacatcga
    53521 actgtttttg caggcattgg aaaatgccaa cgaaaaaaac acatggtggc tcaggcgttt
    53581 tcaatgggca attatgttga tgacgctggt gtcgtctgta ctgatgctgt tttggcacca
    53641 gatttgggtt atccggccgc tgcaggcgtt aagggaaggt gcggaacgca tcggacggag
    53701 gtgtttcgat attccggttc ccgaaggcgg tacgccggaa ttcaaacagg tcgggcgttg
    53761 tttcaatcaa atgggcggca ggttgaaaat tttatatgat gatttggaag gacaagtcgc
    53821 cgagcagaca cgcagtctcg aaaaacaaaa tcaaaacctg accctgctgt accaaactac
    53881 acgggacctg caccaatcct acataccgca acaggctgca gaacattttc taaaccgtat
    53941 cctgcccgcc gtaggagcag attccggcag agtttgtttg gacggcggat ccgatgttta
    54001 tgtttccatt catcatgcgg attgcggcac agcagcttcg gatttgggga agtaccatga
    54061 ggaaatcttc cccattgagt accagaacga aacattgggc aggctgttgc tcagcttttc
    54121 agacggcatt tccctcaccg gagacgaccg cacgctgctt caaaccctga ttcggcaatt
    54181 gggcgtatcg ctcgctagcg cgaagcagga agaagaaaaa cgcctgcttg cagtattgca
    54241 ggaacgcaac ctgattgcgc aaggattaca tgacagcatc gcacaagcat taacgttcct
    54301 aaacctacag gtacagatgc tggaaaccgc ctttgccgaa aacaaacggg aggaagccgc
    54361 agaaaacatc agcttcatca aaacaggcgt gcaggaatgt tatgaagatg tccgcgaact
    54421 gctgctcaac ttccgtacca aaatcagcaa taaagaattt cccgaagccg ttgccgacct
    54481 attcgcccgc tttacgcaac aaaccgggat aacggtcgaa accgcctggg aaaacggttc
    54541 gttcctgccg cctcaggaag cgcagctcca aatgattttt atcctgcagg aaagcctgtc
    54601 caacatccgc aaacacgccc gcgccaccca tgtaaaattc accctttccg aacacggcgg
    54661 acgctttacc atgaccatcc aagacaacgg acaaggtttc gacacggaga aaataggaga
    54721 acccacgggc agccatgtcg gactgcacat catgcaggag cgtgccaaac gcatccatgc
    54781 cgttttagaa atccgttccc aagctcaaca gggaaccacc gtctcattga cggttgcatc
    54841 tgaagaaagc ttgaaatgac tattaaaatt attctgatag acgaccatac cctcttccgc
    54901 agcggcatta aagccctttt gtcgcgccaa cacggttttg aagtcatcgg cgaagccgca
    54961 gacggcctct cgggcatcaa aatgattagt cggctgcaac ccgatgtcgt cctgctcgat
    55021 ttggatatgc ccggcatgaa cggacgcgaa gcactctccc aaatcatcag catcaatccg
    55081 cagcaggcag tgattatgct gaccgtttcc gaagacagcg acgacttgac cgaatgtatt
    55141 cgcatcggcg cgcgcggtta cctgctgaaa aacatcaacg ctgactttct gctcgaaagc
    55201 atacgcaaag ccgccgaagg cgataatgta ttctcgcccg agatgaccgc caaactcgtt
    55261 aaaagcctga tttcccccca acctgcccaa gggacacagg cactctcctc acttacccct
    55321 cgcgaactgg aaatcttggg ctatctcgcc gcaggacaca gcaacaaaat catcgcccgc
    55381 cacctcgatc ttgccgaatc caccgtcaaa gtccacgttc aaaacctgct ccgcaaactc
    55441 aacctcagca gccgggtgca ggccgctgtt tacgccatcc ggcacaacgt cccccaaccc
    55501 gtgctggaat aaggcgggtt cagacggcat attaggggtt ttaatccccg tacggtcatt
    55561 cggataacag accaagcatg taagtttatg cccccataag tacgcttggc atagcagtaa
    55621 tattgttcgg ttcagtgttt tccgtttgcc cctatctgat actgcaatat cagctatgcc
    55681 gtctgaaaac gcatcatcat gatattttca gacggcataa taaaaagcgg aaatactaat
    55741 gcagggtaaa atgttccatt ccgaatccca taaatataca atggcttata tcgtttaagc
    55801 atgtgtaacc caccctcata tcaatcaata tatagtggat taacaaaaac cagtacggcg
    55861 ttgcctcgcc ttgccgtact atctgtactg tctgcggctt cgtcgccttg tcctgatttt
    55921 tgttaatcct ctataaaaat gccgtccaaa cccatgtttg agacggcatt tcgctataga
    55981 agcaatcagg caacctgggt ttgatgctcg tctccctgac gctcacggat caaacctaaa
    56041 cggtaaactg tttcaccttg ttcacccaag agaccctgaa ccgcatcggc atcttcggca
    56101 gcaacaataa cgaccatgcc gatgccgcag ttaaaggttc ggtacatttc ttgggtttcc
    56161 acattgcccg ccttttgaag ccattggaag agcttgggca attcccacga tttagcatcg
    56221 atttgtgcaa ccgtgttttc aggcaacacg cgcggcacgt tttcggtaat gccgccgccg
    56281 gtaatgtgtg ccataccttt aatggtaaat ttttccaaag cggcaagaat cggtttcaca
    56341 tacagacggg tcggcgcaat aacagcctcc cgcaaggttt tgccattatc aaactcggca
    56401 tccagatcgg gattgtcgcg ttcgatgatt ttacggataa gggaatagcc gtttgaatgt
    56461 gcgccgttgg aagccaaacc caataccaca tcgcctacgc cgatgctgcg gccggtaata
    56521 acattctctt tttccaccac gccgacggca aaacccgcca aatcgtattc tccgacggga
    56581 tacatacccg gcatttcggc agtttccccg ccaatcaggg cgcaaccgga ttcttcgcaa
    56641 ccttgggcaa tgcctttaat aacatcggtc gcgcgcggaa catccaattt accgcaggca
    56701 aaatagtcca agaaaaacaa gggctcagcc ccttgaacca aaatatcgtt gacactcatt
    56761 gcaacaaggt cgatgcccac cgtatcgtgt ttatcccaat caaaggcaag tttgagcttg
    56821 gtacccacgc cgtccgtacc gctgaccaat acgggatttt tatatttctt gccgatttcg
    56881 accaacgcgc cgaagccgcc caaatccccc aatacttccg ggcgcatcgt gcgtttggca
    56941 aacggtttga tgttttcgac cagttggtcg cctgcgtcga tatcgacacc tgcatcgcgg
    57001 taactcaatg aagtactcat cgtttttcct tggtaaatgg agattggacg gtaaaataac
    57061 ggggcgtatt ctaccttatt tcacgtttgc aggttcagat ttttagacaa tattgtaaac
    57121 aatccgccat atgcccgcgc gtgtcgggtt tggcgggacc gtccgcagga ttaacgggca
    57181 gaaacccgcc tgcccttccc ctcaattcct tatatatcgc gttccatcaa aagacgcatt
    57241 gcttttctta accattcctt ttggcagacg ggcggaaggg gttttttgat gccatcatca
    57301 aaatcaatat tttcttcttt ccggttgaaa ccccggcatt taggggcagt gaatctgatt
    57361 gcgggcggaa gcacccgttt ccgattcggt gcggaacaaa tggcggcact ttatgtaccg
    57421 ttctgcgtgt tgaaacatat aggcagataa aaaagccgcc cgctgaaaag cagacgactt
    57481 atgttttgtg gcactaattt gtcccgataa gcattaacta tatgatttat ttatcattat
    57541 tggtgcggac ggagagactc gaactctcac acctctcggc gccagaacct aaatctggtg
    57601 cgtctaccaa tttcgccacg tccgcatggg aattggacaa ttatacagat tttgtttttt
    57661 tgtgcaaggt tttcggcggg gctgttgatg gcttggggtt tggggcggta aaatctgttt
    57721 ttcgtccgcc tgacatcgga atcgggcggt tttttgtttt tattgacgga atttgggtat
    57781 gcctgctgct ttgattaagg attttctgct gactcagggt ttgaagctgc cgcttgacga
    57841 ggttcgggcg gcgtatctga cggcgcagac ggtaatggat atggggacgg cttcgattga
    57901 gcgttcggtt ttgtggcttg agcatgagga ttggaggctt gccgattatt tggagcaaaa
    57961 cagcgggaat gaggaaggtc tgaaacggct tttcatggct ttggattcgg tgttttcacg
    58021 ctgtaaaggc gtgcgcgccg cagccgttta tgccctgatg ccgtctgaaa atgccgcctt
    58081 tcggctggtt tgcctgtccc ggcagggcga ggttttggaa aacctgtggg atttggatga
    58141 agcggcaggc aaggtttcgc tggcttgccg ttcggcgcaa agcggctgga tgaatattgc
    58201 ctcggatgtg cgtcgttggt tggatttggg agagctttcg ggagaacgca atcatgcttc
    58261 ggcggcgcaa atttccattc cggtctgcac ggaaagtggc ggtgtgttgg gcgtggttca
    58321 tgtggaattt gaatgcgcag agtgtgcggg tacggcagca caggtggaat gggtggctct
    58381 tgccttggct ttgtccgaac ctttgaaact gctgttgggt atgtctgccg gaaaagatgg
    58441 gagtgaagat gtctgaaatg ttgaaccatg tggcatcctg ccgcctgccg accgaatggg
    58501 gcgtatttac gatgcacggc tttgaagagg caaatgggca ggaacacgtc gcgctgaccg
    58561 tcggtgattt ttcagacggc aatccggttc tgacgcgcat ccactccgaa tgcctgacgg
    58621 gcgatgcgct gttctcgaga aaatgcgact gcggacctca acttgaagcg gccatgaggg
    58681 ccgtacagac agaggggcgc ggcatcatcg tctatctgcg ccaggaagga cgcggcatcg
    58741 ggctgattaa caaaatccgc gcctatcatc tgcaagacca aggtatggat acggttgaag
    58801 ccaatttggc actcgggctg cccgtcgatg cccgcgattt ccgtttggcg caatctatct
    58861 acgaatatct gggcatccgc tcggtcaaac tgctgaccaa caaccccgaa aaaatccaaa
    58921 ccctgaaaga tgcggggatt aacgtggtcg aacgcatccc cctgcacgtc ggggaaaacc
    58981 ttgaaaacaa acgctacctc caaaccaaag cagacaagct ggggcatctg atgtcggaat
    59041 aaggcaaaga tgcagggaac gggcatcctg cgccgccttt cgggaaacag gtttccatac
    59101 cttgataaag caataagttt tataaagtta cagggtgcgg atgcaaacgc attgcgggtg
    59161 cgggtttgag gcatacgcgc aaacatctta atataatgga ttgatattta tgattttctc
    59221 catcatcgtc cctatttaca atgtggaaaa atatctccgc tgctgcgtgg attccgtgct
    59281 tgccgaaaat tttgccgatt atgaaatgat tttggtcgat gacggttcgc cggacggctg
    59341 cgggaagatt tgcgacgaat atgcaggcaa atatccgcat ataaaagtga ttcatcaaga
    59401 aaacggcggg ctgtcggatg cccgcaacgc cggtatccgg gcggcaaaag gcgattacct
    59461 aatctttttg gacagcgacg actattgggc cgacaccaat cgttcaaaaa acgcgcgggg
    59521 ggggggggat tctctttgat ttacaacaac ttgcagacaa aaaggttgat ttgatcctgc
    59581 atccctcgtc cttcaattac cgcgacatcc ccaaaggggc ggacttttcg gataatgatt
    59641 ttgtccgcca ttttgaaacg ctggtggagg ggcggtacta tatcgccaac gcgtggacaa
    59701 aaatcgtcag gcgggaaata atcatcaaaa acaatctgtt tttcccaaaa ggatacattc
    59761 acgaggattt cccgtacagt ttgcaattgg cgcgttttat caagactttt gccttttacg
    59821 ataacccttt ttaccagtac cgcgttctcg gcggctctat cagccacaac atcaaataca
    59881 aaaatttcag cgatgtgctg acgcatctcg accggggtgt ggatttttta gtcgaaaaca
    59941 aaaattcccc catctacggc ggtttgcaaa aatttgtctt cgacaatatc ggctatctga
    60001 ggtcgatatt agtaaggctt tatttttcca aaaacattat cgtcatctac cggaaatatt
    60061 tttcatttaa agaaaaatgc agaaagatat tcggcgcgaa ggcaatccgt ccggttttta
    60121 tcgggaagac cgcattcatc ataggattgc cgatattgcg cctgctcgta ccgcctatgc
    60181 tgtacccggc aatcaaggcc gtttatcaga aatttttttc ggaataagct tccggcaaac
    60241 cccgaatcgg aagcgggcgg gaagaaacag ccgccccgcc ggcggggatt gcggcaatgc
    60301 cgtctgaagc cacgaatccg gcttcagacg gcatctgttt accaaaaagc aaatattcgg
    60361 tttggcgaaa aaaacagatt tgctttttgg taaatacgcg attacaatcc gctacatccg
    60421 atttctacaa aggatgaaac gatgaccgac acagccggtc tgcgccgcca caacctgcgg
    60481 cagtggatag aaaaatacta cggcggtttg caaactcgtt ttgctgaagc cgttgccctc
    60541 aacacaggcg aactctccgc ccttttgaaa aacaaatcct tcggcgagaa aaaagcccgt
    60601 aaaatcgaac aggcggcaaa aatgcccgcc ttctggctcg ataccgaaca caccgcccgc
    60661 ccgcccgaac acacaggaaa acacaccatg tcccatattt cccccatccc cgaaatccta
    60721 gccgacatca aagccggcaa aatggtcatc atcaccgatg ccgaagaccg agaaaacgaa
    60781 ggcgacctgc tgatggcggc gcaattcgtc acgcccgaag ccatcaactt catgatcaaa
    60841 cacgcgcgcg gcttggtctg cctgccgatg gacggcgaaa tggtcgaaaa actcgggctg
    60901 ccgatgatga cccaaaaaaa cggcgcgcaa tacggcacca actttaccgt ctccatcgaa
    60961 gccgcacacg gcattaccac cggcatttcc gccgccgacc gcgccctgac tattcaaacc
    61021 gccgtttccc cgaccgccaa acccgaagac atcgtccaac ccggtcatat ctttccgctt
    61081 cgcgcccaaa aaggcggcgt actcgtccgc gccggacaca ccgaagccgg cgtcgacttg
    61141 gcgcaaatga acgggctgat tcctgcctcc gttatttgcg aaatcatcaa cgacgacggc
    61201 acgatggcgc gtatgcccga actgatgaaa ttcgccgaag aacacaacct taaaatcggc
    61261 accattaccg acctcatcga ataccgcagc cgtaccgaaa gcctgctcga agacatgggc
    61321 aatgcgcctg tacaaacccc gtggggcgag ttccaacaac acgtttacgt cgacaaactc
    61381 tccggcgaaa cccacctcgc cctcgtcaaa ggcacgccct ccgccgacac cgaaaccctc
    61441 gtccgcgtcc acgaaccctt cagtgtgatg gacttcattc aagccaaccc gcgccattca
    61501 tggtcattgc ccaaagcact cgagcgcgtc caacaggcag aaagcggcgt cgtcatcctt
    61561 ctgcaccgca ccgaagacgg cgcatccctg ctcgaccgca ccctgcccaa aggcgcaaac
    61621 caagcctaca aatgggacag caaaagctac ggcatcggcg cacaaatcct cgtcggcctc
    61681 aacgtcaaaa aactgcgcgt cctcgggcag ccctcatctt tcaccggtct gaccggcttc
    61741 ggtttggaag tcgtcggctt tgaagaagcg gaaaaataat atagtaaatt caaatacttt
    61801 atatttgctt tatttattgc attatttccg tgcaaacgaa aacccggtct gttgggttgg
    61861 attttgtttt tgcaaatttc gggtaacttc taattcgtca ttcccgcgca ggcgggaatc
    61921 cggttcgtcg ggtttttgtc atttccgata aattcctgtg gctttggttt tttggattct
    61981 ctctttcagg gaaagaacgg cataagtatt ttccaaacca aacaaaatgc cgtctgaaag
    62041 gctttcagac ggcattttaa gtttgaccgg tttcattcat cggtatttat gaattgaatt
    62101 tcaacatcgc caatctatcc ttaatctctt tttccaattc ggcagattcg gcgaaaagtt
    62161 tatccaaatc cgctgaaaat cccgccattt tttgcgcaaa ttcgtcggca gaaatatcca
    62221 cataatcaat ctttacctcg aaatattgcc ccgccgacag gctgtagttt ttggcttgga
    62281 tttcatcgta gccgaccacc acgctgaaat cttccacggc tttcttatgc gtgaaggtat
    62341 cgcagatttt ttgttcttct tcgcgggaaa gtacggtttt ttggtttttg ccgtctttga
    62401 ttttttcgcc caagcccgat gcgtcgatta acaccacttc gtctttattg gctttatcga
    62461 taaacagaat agaaacgttg gttcccgtgg tggcgaaaat attggaaggc atggacacca
    62521 cgccggcgag cattttgttt tccaccaaat attcgcggat ttttttgtcg atgcccgatt
    62581 gggcggtgat aaagcccgtc ggcaacacaa tcgccgcttt gctgttttct tttaggctaa
    62641 acaagatatg ctggataaaa agctggtaga tttccatctt atccgtatct tttgccttga
    62701 ttttcgggat accggcaaaa aagcgttcgc ggttttcctc gccttctagt tggtcgcgga
    62761 aatcgctgaa atccaattta aacggcggat tcgaaacgat gaaatcaaat tttttcagac
    62821 ggcctgaagc gtctttatgg gcgggcgaca aaatcgtatt gccctgcacc acattattca
    62881 aagaatgcac cagattattc aaaatcagat tcagccgtag cagattggac gatttttgcg
    62941 aaatatcctg cgtataaatc atgcatttat cttcgccgat ggcatgggcg acattcatca
    63001 acagcgttcc cgaaccggcc gacggatcgt aaacgtccac gctgcggatt tgcccgcgca
    63061 cttcctccgg caccaaaata tccgccataa tccgcgctac tgcgtgcggc gtgtagtatt
    63121 cggcgtattt gccgcccgaa ttactgttgt agtctttaat caggtattca aaaatcgtgg
    63181 caaaaaaatc aaatttttgg gcaaaaatcg cctcgaaact aaaacccgcc agtttggaaa
    63241 tcagcgcacg gcaaaaatcg tcgcggcggc cttcatccgt aatatattga ctgatacgct
    63301 cgaacaacac gattttcgcc ccgccttcgg tttttaccga aaacagttcc gcattatgtg
    63361 cggcaatatc ggtcagcgta tcgtcaaaga gtttggcaaa gtccgcgccg ttttgtcgct
    63421 ctgataaata cttgatggaa tgttcaggct tcaaaacggc tgtttttccc tcaatatcca
    63481 tattgatgaa ttcgattggc tcgtcgggat tttctttgcg aatttgttta acttcgaaat
    63541 cgtatttgtc gtttaggaat ttatacaaaa atgcctgact gatgattttg aactcgttgc
    63601 cgtcattgcc caaaccatag ttggcgcaaa tggtttttaa gctgtcgatt agggatttgg
    63661 tttgttcggt aaagtgttgt tcggtcattt ttttctcgtt tgttttattt tgtaggtctg
    63721 attcttgaat ttggcttatt ccgccacaga aacctgcccg ttcatcagca tgggaagtag
    63781 gaaatcgcgg agttgggtta gttggtggtt ttgttttata ttatttcgaa ctttaacaaa
    63841 gaaagtattt gctatttcat tatatttttg agaaatatct ttactaggta aaattatttt
    63901 aaattctttt aagaatttga aatgtctagc atatccataa cttgataaat ctatttgatt
    63961 tatgatttgg taaaacaaat agtttggcat tcgttcatta ttgcttaata ttacttgtgt
    64021 accatctgct ccccgcgcat attgaaaatt aactaactta acaattcttg tatgatcacc
    64081 aaaaataata tgagcatctt gcgggtttaa tatagatttt tcatcattgg taaatccaca
    64141 aataaaatct tgactttggt caacaacagg atattttcca aaatccttaa tatctttatt
    64201 taatattttt gtagtatttg ggatttttgc taagcaagat tgtaattcta tacttcccca
    64261 ccccttcgga atttcccgtt tcaaggtttc atcaaacaca atttcgccgc ctgaggattt
    64321 gtagggcttg ccgttggcgt cagggaagtc gaactgcacg aaccagtagt cgtacagggt
    64381 tttcgccatc tcttccaagc gcgcgttgat ttgtttgttc agggcaattt ttttgtccaa
    64441 agtagatagg acagcggcta tggattgttg ggttttgagg tcaaaaactg gaattttaaa
    64501 ttccataatt tgtgttttat cgccccgtgg catctttgta ccctttgcac ctttcattgc
    64561 atggataaaa aaatcatcac gaaacaaagc ataaaataag aaatgactat ctgtttctgt
    64621 attagcacga atcgctaaaa catcagcaga acaacctcca tcaaattggg cataccaaat
    64681 tttttttaga tatggtcgta tattggctaa taaaatatcg ccctttttaa atgaagttac
    64741 ttttccacct tgaattggta aagacgttgc acattctata ccttgcttat tttgcaatat
    64801 attgtctgtt gaaatatatt ggctaagcga aatttcattt gtatcaactt tgttatttat
    64861 atatgttgca tgattcttta atgcactatt catcttaact gtatctctaa aaactcttca
    64921 aaggccgtct gaaaaaacat ttcaaaatcc gaccgcactt ttttaagcag cctgcattga
    64981 tgcctgctat tttttcaggt tcagcagttc ggcaatatgg cgttcgccct cgcgttcttt
    65041 ttccagtctg cgtgcggcgg caaatttttc gtattcgatg cgggcttttt cgtctgcctg
    65101 cgttttgctg attgtccctg ccgtttgtaa gacttggcgg tcattgaagg cgaggaaggc
    65161 atccagtttt tccgcccagt cttgcaggaa tatctgtttt ttcatgcttg cctggtcttc
    65221 ggcaaaatcc agccacatgg aaacgatgcg gttgagggcg tcgatttctg cctcgttcag
    65281 gtaatttttg gcaacggtta catcggcttt ggcgatttct ttgccccgcc aatgggtcag
    65341 ccccatattg ggtttccccg catctgccct ttggtaaata atttcagggg cggtataacg
    65401 ggtacaggcg taatgaagtt tgttttgtat gattttgaaa aaatgggtgg tttctttgaa
    65461 gctgggctgg taatccgccg ccatggcgaa tatttcgcgc acacgcagat acatccgccg
    65521 ttcgctgctg cggatgtccc tgatgcggtt gagcaactgt tcaaactgat cttcggcacg
    65581 gtttgtcccg acaggcgggt ttttcagccg gtcgtcgttt aatacgaagc ccttgctgat
    65641 gtattcgccc aatgtccgtg ttgcccattg gcggaattgt gtaccgcgtg tggagcggac
    65701 gcggtagccg acggcgatga ttagggagag gggataaagc cccatgcggt agttcttgcc
    65761 gtctgcagca gttgtaaact gaaagtttac aactgaatcg tccagttctt gttctgcaaa
    65821 tatggctttg atgtgtttac cgatgttttg tttgctggtt tggtagagtt cggcaatttc
    65881 tttttgggta agccatagtt cgccgtcaag ctcgcgcagg gtaaattcgg atagtccgtc
    65941 ttccgtggtg tagatgatga tgtcgttgtt gtgcatagct ttcctgtatt tataaatggt
    66001 taaggctgcc tgaaaatcta ctcctcaaat ccgcccgctt tcttttaaat attcccgcac
    66061 caacaggcgg ttgatggctt ggatgtctgc gggttgaacg ggaaattgct gttccgttct
    66121 aaagcgtttt aatacgatgc cctgcatttg tttttcaaag taattttggt tgtccaaaat
    66181 ctgctccata tccagcactt tttggtccgt gtcggttttt acaccattca gcgcgttaaa
    66241 gactttgagt ttatcgccgt agagggcggg gttttcgcgc aggcgtttgt ggatgcgggc
    66301 atatttggcg tcgccgccgt atttgtgccg caagaggtcg ttttggcggt tcagctcttt
    66361 gattttggca tagacggttt cgagtgtggc gatgtttgcc tgcatttctt cctgcccgac
    66421 ttcggcgagg ttctttttct tgaagatgcg ttccagctct tcgcgtaggc tgatgtattc
    66481 ggggtcgcct tggtcgaagt tgcccgccag tccttcgcgg actttgcgca tgatgtcttt
    66541 taaatcgtcg gcaagtttga gttccgcctc gccgattttg acgaattgaa agtaaacgtc
    66601 ttccagcgct tcattgagca aatgggcggt gtcgccttgc tgtaattttt cggcaaggtt
    66661 taaggtatcc aagcgggcag cggtttcgcg gtacaaaaga ttgagcttgt caaaatccag
    66721 atgcgcgaga aattcgtggc taccttgcag gcgcaggatg ttgtacaact ctttggcggt
    66781 ttgcagggct ttttttaggg taagtagctg ttttttatct tctatttggc tgatttggca
    66841 gcaaaattct tcggcgtttt cggtatcgaa atcaaacagg gcgtttttaa tgtctgcaat
    66901 ttcctgttcg atttcttcgg cagttttgaa aagctggctg taactgccga tttcgtcacc
    66961 caattcatta gaaagttcat cccaataggc gcggttggtt ttgtcgaatt cgcgttcgat
    67021 gtcggcaaaa tcgacaacgt agccgtaacg gtaagatttg taggtgcggt tgacacgggt
    67081 cagggtttgc aaaagattgt gggctttgat gagcctgccc aaatagagtt ttttcaggcg
    67141 cggcgcgtca aagccggtca aaagcatgtt gtacacaaac aagatgtcga ttttgcccgc
    67201 tttgaaatct ttaacccatt ggtcgcgttc gtccttagtg ccgacgtcgt gcaatatcag
    67261 cgcggcggtg aaattatggt ctgaggctat ctgaaaatgc tcaaaaagct gacgtgcctg
    67321 ttccgcgctg tcgcaaacaa ccatagcgcc gaggctgtcg tcttggttgg tttttctgaa
    67381 tttggcgaag tcgtccaaga tgtagtcaag catcgggtgg acgaagtgcg gatgggcgta
    67441 gatttctttg cggtcaaagc tgcctttttc gatttcaagt tgcgccagtg cttcttgtaa
    67501 ttgcgccttg tatcggctgc cgatttcttc gcgtatcagg cgcagggtgt agccgtcggc
    67561 aatggaggcg ttgtaatagt atttgtggat gtagtcgccg aagagttcgc gggtgttgac
    67621 gttgcctgcg gttacgccga taagcggcgt gccagtcagc ccgattttga cggcgtttac
    67681 gtcggactgg ttgaggttgg caagaaacga gcctttgggg ttgtagctgc ggtgtacttc
    67741 gtcgagaaaa tagacgcgct gaatggcgag gtcgtagtcg ttacgggcga cgacatcggg
    67801 gtcgtctttg aatttttgga tgttgacgac agtgatttcc gctttacctg cgtggttgtg
    67861 cagggtttgg gcggatttga tgtcggcagc gaaggcttcg cggctgttga tggtatggac
    67921 gattaagtta cggtcggtaa attcctgatg cgcctgtttc aataaatcga gcctgtccac
    67981 gataaagtag aatttcggca cgatgccctg tttggcgtaa tagtgggtca ggtagcgggt
    68041 gttgtaatag gcaagcgcgg ttttgccgga gccttgggtg tgccaaatca cgcctttttt
    68101 gccgccgttt gccaaatgtt tttcaatggc gagggtagcg aatagctgcg gatagcgcat
    68161 gatgtgtttt tgcagcgcgc cttggtcggt tttgacatac gtcagcccat gttgcaggag
    68221 aaaggcgagg cgcttgcggc aaagcagcga ggtcaaaatg cggttggtcg gcgtatcggg
    68281 cgatttgttg ctgacgaatt cggggctgtg tttgataacg ggcaggttgt tgtcttgcaa
    68341 gactttgttt tcgaagtcgt ctgaaagcgt gttcaataat tcggcaagat ttaatttgtg
    68401 ctcttcgcgg aagtagttga acactggctt gccgtaagca gacgaagcgt aaaacacgcc
    68461 ttgtgccggt tcggttgcgc cgtcgtcgta ttccatgttg ttggaaaaaa tcatgaactg
    68521 ggtgatgttg atgaaacggc ggaatttggg atttttcgca cgtttgccca tgcgctcccg
    68581 ctcttctccg atgccgcctt tattgttggg ctttttgact tcgataaaga ccaaaggcat
    68641 gccgtttacc agcagggtaa tgtcggggcg gaattcttca tcgccgttga tacagggcaa
    68701 ttcggttaca acgtggaagc tgttgttatc aaaattttgg aagtcaatca gtttcttgcc
    68761 gccggattga ttggtaaggc gttcgtaaaa cttttgcccc aagtcttcgt tgtccagttc
    68821 taagcgtatg tcggtcagca aacggcgtgc atcgtccggc gacaaatcgg gattgatgcg
    68881 gcagaggctt tctataaaga ttttgggaaa gatgttggtt ggtttatccc aattttcgtt
    68941 tttgagcgag aggtagtcgt agcccaagcg catcaggtgc aaaacggcgg ggattttgac
    69001 gcgggaattt tcgttgtgag tttgggacat aaacgtaatg attcatcaaa atttattgaa
    69061 acggattata acacctgaaa aagccgatac gcctttttgg tgtaccggct ttgccatacc
    69121 gttctgcttc agacggcatt gcttcatttt gcctttaatg cctcttcatc cagcgatttg
    69181 agccacgcca gcttttcgcc gattttgatt tccaacccgc gcgggacggg ttggtagaag
    69241 tccggttcgt ccaagccgtc gggcatatag ctttcgccgg cggcgtaggc gttcggttcg
    69301 tcgtgggcgt agcggtattc gcgtccgtag cccaattccg tcatcaattt cgtcggggcg
    69361 ttgcgaagat ggacgggcac ttcatcgctg ctgttttctg caacaaatcg gcgcatttgt
    69421 ttatacgctt tgtagcccgc gttggatttc gcggcggcgg caaggtacag cacggcttgc
    69481 gccaaagcca gttcgccttc gggcgagcct aagcgttcga aggtggcggc ggcatcgttg
    69541 gcgatttgga aggcgcgagg gtcggcaagc ccgatgtcct cccacgccat gcgcacgatg
    69601 cggcgggcga ggtagcgcgg gtcggtgccg ccgtcgagca tacggcagaa ccaatacagc
    69661 gcggcgttcg gatgcgaacc gcgcacggat ttgtgcaggg cggagatttg gttgtagaaa
    69721 ctctcgccgc ctttgtcgaa acggcggatt tgcgccccga gactgtcggc gagaaattcg
    69781 gcggttaagt ttttcagacg acgtgtatcg gcggcgcgta aaagttgttc caacaaattc
    69841 aacaatctgc gcgcatcacc gtcggcggta ttcacgagta atttttgcgc atccgtttca
    69901 atcgtaaact cttggtattc aggcaaagcc aataccttgg caatcagctt tttcaggtcg
    69961 tctgaagaca agggttgcaa aacatacacc tgagcgcggc tcaacagcgc gggattgact
    70021 tcaaacgacg gattttccgt cgtcgcacca ataaaggtta gcaaaccgct ttcgacatgc
    70081 ggcaaaaacg cgtcctgctg cgccttgttg aagcggtgga cttcatcgac aaacaaaatc
    70141 gtcgcgcgtc cctgctgcaa agcgatttcg gctttatcga ttgcctcgcg gatgtccttc
    70201 acgccggaaa atacggcgga aacaggcaaa aactgggcgt tgaaactctg cgccaaaatc
    70261 tgcgccaacg tcgtcttgcc cacgcccggc ggcccccaca gcaacataga atgcggcttg
    70321 ccgccttcta ccgccacgcg caaaggttta ccttcgccga tgaggtgttc ctgccccacc
    70381 acgtcgtcaa gcgtatgcgg acgcaatcgt tcggcaagcg gcgcgtcggg ttctcgggca
    70441 aacaaatcgg tcataacggc tccgtcaaca ggttttcaaa caatatgatt atacggcagg
    70501 gaacggcggc gtgccgcata cggattccgc cccgccgttt gccttaagcc gatattaggc
    70561 gcatactgga aaagacgaga gacttcacac aatatatccg gcacggagac cgattccgca
    70621 ccggcatgac aatacccaaa tcagcgtttc aattaaacat taaggagact aaaatagaaa
    70681 atttgcttta tctaccattg ctttgttgat tcaatcggca ttatgttttg aggcggaagc
    70741 ccatgaatat actaatattc aagagatgga atgggtgtct ttattttctg atccgcaaag
    70801 agacgatgat agtcttataa cccttaaaga tgaaaaaatc actgtaaaaa actatattgt
    70861 gccttggtgg aaaaaaggtg aaaactttag aaaattagaa cttattacca ctggtaaaaa
    70921 tgataatgga aatttaacgg taaatggaat ttacggcatc caatcttttg cagactctca
    70981 attgggatat acgcctgaat tattggtaaa aagcaataat gatattctat ttaaaaataa
    71041 tttccaatct gttgacgtga agccccattc cggaaatatc catttggatg ccaaaggaaa
    71101 aattcaattt aatttgaatg ttcccgatgt tgatggggta caagatgtag ccagtggaaa
    71161 aaaataccga caaacaatag tgccttagtg cggtatgtca acactatcag ccccccgatg
    71221 aaaatgacaa aggcagcata tcctttcatt caaaaacagg caatttgttc aatatgaacg
    71281 ttaaagttca taaagacaca caccatttcg gtattcacaa cctcaacaat accgatgttc
    71341 atctgactgc cgaagacagc gataatgtga ttgagattaa aggggaactc gccgacctta
    71401 tgccgacagg aaatgttgag agtacaagtt ttttaagggg cattgaaacg gcttttaaat
    71461 ccaataccgt attaacgggg aataacaata aaatatccat cgccaccgag ggcaagggct
    71521 tgggattggg cgtaaatggt ctccagtcca actcccccta ttccggacac acgacgggca
    71581 ttttcatcaa taatcaatca agcgacctta cccaaccttc ccttgatggc atcagcacca
    71641 tcaagctcaa agccgtcaag ggaaataatg aagttgattt aaatgtaaaa aaccacgcct
    71701 ccgtaaaagg cattatcgcg tcgcattctg caaaagcaac gcttgaagcc gaaggggaca
    71761 atattgtcta ggttaaaaat ccggacacca atacggcttt aatcgatgct ttgaaaaaac
    71821 aataccccaa tgcttcgaaa gaactataca gggtgggggt tcaatccacg actggaacag
    71881 tacgcatttc gccaaacggc aaaatcgcat taacggaatt gaaaactttt ggaattgtac
    71941 aaaacggcat ttgcgtcagt ttatagtgga ttaacaaaaa ccagtacggc gttgcctcg
//
LOCUS       DAWFJR010000002        47019 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWFJR010000002 DAWFJR010000000
VERSION     DAWFJR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 47019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47019
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.002"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            438..1652
                     /locus_tag="WGR04_000075"
     CDS             438..1652
                     /locus_tag="WGR04_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ0793717.1"
                     /translation="MSEVEYFSHFISDGKGKLLEIPQRRGKQDGVFVDWISFTFHEDT
                     LLKVSGCPLFSDAEYMYVLSRKLEEILGFGITRKCKSRGNKFYESMYRLGSDDVDYGE
                     VHFGGQRNTVLVELKGTGCSVASPGWELRLKQFLDDSIRTRITRIDLALDFFDGEYTP
                     DQALLDHDNGFFDNSNQRPKSEMIGTAWRNEDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNYGDIEIPLDILINQGSYFCGAFPICRKFKNMPVPERFDQRKKK
                     LNLTFEHKLHYAKNAVGKLVNFMIEMGFDNSEIVESLKADSGFPKGLEPEKYALEMLR
                     DGLKHGFIHEQPDIDLEIELDELGVIAFKNSDKFDREKRLFSPDYDVEKERKYQEYLS
                     KVYHQNVDYDYF"
     gene            1664..1975
                     /locus_tag="WGR04_000076"
     CDS             1664..1975
                     /locus_tag="WGR04_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793718.1"
                     /translation="MFNQTQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVVTPLPAQS
                     GNAVGFTAAQMKFGDSKNFSRLENLKYPCEVMVMVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            1979..2179
                     /locus_tag="WGR04_000077"
     CDS             1979..2179
                     /locus_tag="WGR04_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793719.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEMGGGFQIFQFFVKSE"
     gene            2417..2647
                     /locus_tag="WGR04_000078"
     CDS             2417..2647
                     /locus_tag="WGR04_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793720.1"
                     /translation="MNIVKKYAVKALLAAGIFTPAIVMADGFDASAIGTQVANVIMGF
                     VAMVSAVGMAAITVILAIQGFKMAWSMIKSVK"
     gene            2675..2977
                     /locus_tag="WGR04_000079"
     CDS             2675..2977
                     /locus_tag="WGR04_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793721.1"
                     /translation="MGYRVGINCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGDKW
                     ILNGKPVTLSYPECSNFEQIKQGSYVGSTVLILFVVIYGFRLLINFLKDIGKVGTD"
     gene            3324..4937
                     /locus_tag="WGR04_000080"
     CDS             3324..4937
                     /locus_tag="WGR04_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ0793722.1"
                     /translation="MFIILGRNFLKIILCFSFFVSKFALASVNAPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGLFDRESGRYLTSEAQDLKVRHVSTGASSTGKVSSVVSSSVS
                     RAGVLAGVGKLARLGAKFSTRAVPYVGTALLAHDVYETFKEDIQAQGYQYDPETDKFV
                     KGYEYSNCLWYEDERRINRTYGCYGVDSSIMRLMSDYSRFPEVKELMESQMYRLARPF
                     WNWHKEELNKLSSLDWNNFVLNRCTFDWNGGDCVVNKGDDYRNGANFSLSRNPKYKEE
                     MDAKKLEEILSLKVDANPDKYIKATGYPGYSEKVEVAPGTKVNMGPVTDRNGNPVQVV
                     ATFGRDSQGNTTVDVQVIPRPDLTPGSAEAPNAQPLPEVSPAENPANNPNPNENPGTS
                     PNPEPDPDLNPDANPDTDGQPGTSPDSPAVPDRPNGRDGKDGGLLCKFFPDILACDRL
                     PEPNPAEDLNLPSETVNVEFKKSGIFQDSAQCPAPVTFTITVLDSSKQFAFSFENACT
                     IAERLRYMLLALAWAVAAFFCIRTVSREV"
     gene            5240..5524
                     /locus_tag="WGR04_000081"
     CDS             5240..5524
                     /locus_tag="WGR04_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ0793723.1"
                     /translation="MKLLAALIPLLMSVVGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     HSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            5525..6712
                     /locus_tag="WGR04_000082"
     CDS             5525..6712
                     /locus_tag="WGR04_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HEZ0793724.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGIRRKVFTNI
                     KGLKIPHTYIETDAKKLPKSTDEQLSAHDMYEWIKKPENIGSIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGSKLLDQNLRTLVRKHYHIASNKMGMRTLLEW
                     KICADDPVKMASSAFSSIYTLDKKVYDLYESAEVHTVNKVKRSKWFYTLPVIVLLIPV
                     FVGLSYKMLSSYGKKQEEPAAQESAATEQQAVLPDKTEGEPVNNGNLTADMFVPTLTE
                     KPESKPIYNGVRQVKTFEYIAGCVQGGRTGCTCYSHQGTALKEVTELMCKDYVKNGLP
                     FNPYKEESQGQEVRQSAQQHSDRAQVATLGGKPLQNLMYDNWQERGKPFEGIGGGVVG
                     SAN"
     gene            6927..7883
                     /locus_tag="WGR04_000083"
     CDS             6927..7883
                     /locus_tag="WGR04_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0793725.1"
                     /translation="MNIIGLDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKAYIGMEATGIYYEKAADMLSSYYTVYVINPLKIKDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLQQQQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDYNHYRNLQTIPSIGKDTALVLYAQLTEKHFKT
                     ANQFVSYAGLSPAIIQSGTSVRGRGRLSRYGNRRLKSTLYMPALCAYRFNAFPKLINN
                     LKKAGKPKMVIIVAIMRKLAKLAYYIVKTGQPYDAERHRLNQ"
     gene            complement(8339..9112)
                     /locus_tag="WGR04_000084"
     CDS             complement(8339..9112)
                     /locus_tag="WGR04_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ0793726.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVKSATVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETAIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELMARVSGK"
     gene            complement(9211..9834)
                     /gene="rsmG"
                     /locus_tag="WGR04_000085"
     CDS             complement(9211..9834)
                     /gene="rsmG"
                     /locus_tag="WGR04_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ0793727.1"
                     /translation="MERKERLRAGIAALGLDISAAAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTAHLLKDG
                     GYWAAMKGVYPQEEIDRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            complement(9930..10415)
                     /locus_tag="WGR04_000086"
     CDS             complement(9930..10415)
                     /locus_tag="WGR04_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793728.1"
                     /translation="MFVNEKDPYAALFAGLVFLTLPFALAVHDAFALAFGRTGLLVSV
                     SDGGFGWRGGWDGVVWFVFGVFAFLNVVVSAGLTKLVYKKMMRRHSRYTLFLSGVAAC
                     AAAAVAWIFELLLGSGALGGLRGEAVLEYAFAVWLVAMLTLPKRLTRAPVQPVVFHRK
                     K"
     gene            10521..10596
                     /locus_tag="WGR04_000087"
     tRNA            10521..10596
                     /locus_tag="WGR04_000087"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10554..10556,aa:Thr,seq:cgt)
     gene            10672..11799
                     /locus_tag="WGR04_000088"
     CDS             10672..11799
                     /locus_tag="WGR04_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ0793729.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHAVRLGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEENMAKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQAPKLNSGE
                     IRLLDRHFTQLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHS"
     gene            11980..12537
                     /gene="frr"
                     /locus_tag="WGR04_000089"
     CDS             11980..12537
                     /gene="frr"
                     /locus_tag="WGR04_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ0793730.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            12593..13339
                     /locus_tag="WGR04_000090"
     CDS             12593..13339
                     /locus_tag="WGR04_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ0793731.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDET
                     ALDAAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            13342..14139
                     /locus_tag="WGR04_000091"
     CDS             13342..14139
                     /locus_tag="WGR04_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ0793732.1"
                     /translation="MLKQRVMTAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLSGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            14144..15379
                     /locus_tag="WGR04_000092"
     CDS             14144..15379
                     /locus_tag="WGR04_000092"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ0793733.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPA
                     QAVKHPNWSMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLAQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            15414..16754
                     /gene="rseP"
                     /locus_tag="WGR04_000093"
     CDS             15414..16754
                     /gene="rseP"
                     /locus_tag="WGR04_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ0793734.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYAVAKLCGVKVLRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            16811..19204
                     /gene="bamA"
                     /locus_tag="WGR04_000094"
     CDS             16811..19204
                     /gene="bamA"
                     /locus_tag="WGR04_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ0793735.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYNRVNFGLVAEHLTVNTYNKAPKHYADFIKQYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            19270..19770
                     /locus_tag="WGR04_000095"
     CDS             19270..19770
                     /locus_tag="WGR04_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ0793736.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            19803..20849
                     /gene="lpxD"
                     /locus_tag="WGR04_000096"
     CDS             19803..20849
                     /gene="lpxD"
                     /locus_tag="WGR04_000096"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ0793737.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANVYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            20883..21332
                     /gene="fabZ"
                     /locus_tag="WGR04_000097"
     CDS             20883..21332
                     /gene="fabZ"
                     /locus_tag="WGR04_000097"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ0793738.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            21539..22219
                     /locus_tag="WGR04_000098"
     CDS             21539..22219
                     /locus_tag="WGR04_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEZ0793739.1"
                     /translation="MERYKNAVGEDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CLQQAFKDRNWLYNSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDATGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINKFRNRLAHHEPICFKGAIKDTNYARNIHQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            22262..23038
                     /gene="lpxA"
                     /locus_tag="WGR04_000099"
     CDS             22262..23038
                     /gene="lpxA"
                     /locus_tag="WGR04_000099"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ0793740.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNTSLAGHVTVGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFTQSTRGIIR"
     gene            23395..24786
                     /locus_tag="WGR04_000100"
     CDS             23395..24786
                     /locus_tag="WGR04_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0793741.1"
                     /translation="MQLFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            24865..26121
                     /locus_tag="WGR04_000101"
     CDS             24865..26121
                     /locus_tag="WGR04_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ0793742.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWQMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEERKKGTLQIFRQTKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCHLFTENLYKLCQEKGVQF
                     HFNQTISRIDHNGLRIKAVETETGRFEADAVVCALGCFSRTVLAQLDLDLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQALFWSGLRPMTPDSTPLIGRTRFENLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            26302..27111
                     /gene="zupT"
                     /locus_tag="WGR04_000102"
     CDS             26302..27111
                     /gene="zupT"
                     /locus_tag="WGR04_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ0793743.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            27208..30045
                     /locus_tag="WGR04_000103"
     CDS             27208..30045
                     /locus_tag="WGR04_000103"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ0793744.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADKYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQQGFALPEKYAGLDRFAARK
                     QMVADLQEQGLLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDETG
                     NVYVARNQAEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLDKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGYSFPDMWIIGRL
                     NQTIEQVTQAYETYRFDLSAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGEIVQTAFEQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFIESADDLADYLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLGKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQTQLSKLKD"
     gene            30187..30378
                     /locus_tag="WGR04_000104"
     CDS             30187..30378
                     /locus_tag="WGR04_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793745.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
     gene            complement(30375..31295)
                     /gene="oxyR"
                     /locus_tag="WGR04_000105"
     CDS             complement(30375..31295)
                     /gene="oxyR"
                     /locus_tag="WGR04_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ0793746.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(31299..31562)
                     /gene="minE"
                     /locus_tag="WGR04_000106"
     CDS             complement(31299..31562)
                     /gene="minE"
                     /locus_tag="WGR04_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ0793747.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            complement(31566..32381)
                     /gene="minD"
                     /locus_tag="WGR04_000107"
     CDS             complement(31566..32381)
                     /gene="minD"
                     /locus_tag="WGR04_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ0793748.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            complement(32410..33123)
                     /gene="minC"
                     /locus_tag="WGR04_000108"
     CDS             complement(32410..33123)
                     /gene="minC"
                     /locus_tag="WGR04_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ0793749.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            complement(33270..33638)
                     /gene="rplQ"
                     /locus_tag="WGR04_000109"
     CDS             complement(33270..33638)
                     /gene="rplQ"
                     /locus_tag="WGR04_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ0793750.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            complement(33662..34648)
                     /gene="rpoA"
                     /locus_tag="WGR04_000110"
     CDS             complement(33662..34648)
                     /gene="rpoA"
                     /locus_tag="WGR04_000110"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ0793751.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            complement(34674..35294)
                     /gene="rpsD"
                     /locus_tag="WGR04_000111"
     CDS             complement(34674..35294)
                     /gene="rpsD"
                     /locus_tag="WGR04_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ0793752.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            complement(35314..35709)
                     /gene="rpsK"
                     /locus_tag="WGR04_000112"
     CDS             complement(35314..35709)
                     /gene="rpsK"
                     /locus_tag="WGR04_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ0793753.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            complement(35729..36091)
                     /gene="rpsM"
                     /locus_tag="WGR04_000113"
     CDS             complement(35729..36091)
                     /gene="rpsM"
                     /locus_tag="WGR04_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ0793754.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            complement(36157..36270)
                     /gene="rpmJ"
                     /locus_tag="WGR04_000114"
     CDS             complement(36157..36270)
                     /gene="rpmJ"
                     /locus_tag="WGR04_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ0793755.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            complement(36291..36509)
                     /gene="infA"
                     /locus_tag="WGR04_000115"
     CDS             complement(36291..36509)
                     /gene="infA"
                     /locus_tag="WGR04_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ0793756.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            complement(36514..37824)
                     /gene="secY"
                     /locus_tag="WGR04_000116"
     CDS             complement(36514..37824)
                     /gene="secY"
                     /locus_tag="WGR04_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ0793757.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            complement(37836..38270)
                     /gene="rplO"
                     /locus_tag="WGR04_000117"
     CDS             complement(37836..38270)
                     /gene="rplO"
                     /locus_tag="WGR04_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ0793758.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            complement(38272..38457)
                     /gene="rpmD"
                     /locus_tag="WGR04_000118"
     CDS             complement(38272..38457)
                     /gene="rpmD"
                     /locus_tag="WGR04_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ0793759.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            complement(38450..38968)
                     /gene="rpsE"
                     /locus_tag="WGR04_000119"
     CDS             complement(38450..38968)
                     /gene="rpsE"
                     /locus_tag="WGR04_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ0793760.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            complement(38987..39340)
                     /gene="rplR"
                     /locus_tag="WGR04_000120"
     CDS             complement(38987..39340)
                     /gene="rplR"
                     /locus_tag="WGR04_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ0793761.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            complement(39354..39887)
                     /gene="rplF"
                     /locus_tag="WGR04_000121"
     CDS             complement(39354..39887)
                     /gene="rplF"
                     /locus_tag="WGR04_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ0793762.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLAFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            complement(39901..40293)
                     /gene="rpsH"
                     /locus_tag="WGR04_000122"
     CDS             complement(39901..40293)
                     /gene="rpsH"
                     /locus_tag="WGR04_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ0793763.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            complement(40309..40614)
                     /gene="rpsN"
                     /locus_tag="WGR04_000123"
     CDS             complement(40309..40614)
                     /gene="rpsN"
                     /locus_tag="WGR04_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ0793764.1"
                     /translation="MAKKALINRNLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            complement(40617..41156)
                     /gene="rplE"
                     /locus_tag="WGR04_000124"
     CDS             complement(40617..41156)
                     /gene="rplE"
                     /locus_tag="WGR04_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ0793765.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            complement(41166..41489)
                     /gene="rplX"
                     /locus_tag="WGR04_000125"
     CDS             complement(41166..41489)
                     /gene="rplX"
                     /locus_tag="WGR04_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ0793766.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            complement(41501..41869)
                     /gene="rplN"
                     /locus_tag="WGR04_000126"
     CDS             complement(41501..41869)
                     /gene="rplN"
                     /locus_tag="WGR04_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ0793767.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            complement(42091..42354)
                     /gene="rpsQ"
                     /locus_tag="WGR04_000127"
     CDS             complement(42091..42354)
                     /gene="rpsQ"
                     /locus_tag="WGR04_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ0793768.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            complement(42354..42545)
                     /gene="rpmC"
                     /locus_tag="WGR04_000128"
     CDS             complement(42354..42545)
                     /gene="rpmC"
                     /locus_tag="WGR04_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ0793769.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            complement(42545..42961)
                     /gene="rplP"
                     /locus_tag="WGR04_000129"
     CDS             complement(42545..42961)
                     /gene="rplP"
                     /locus_tag="WGR04_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ0793770.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            complement(42945..43637)
                     /gene="rpsC"
                     /locus_tag="WGR04_000130"
     CDS             complement(42945..43637)
                     /gene="rpsC"
                     /locus_tag="WGR04_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ0793771.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            complement(43647..43976)
                     /gene="rplV"
                     /locus_tag="WGR04_000131"
     CDS             complement(43647..43976)
                     /gene="rplV"
                     /locus_tag="WGR04_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ0793772.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            complement(43985..44263)
                     /gene="rpsS"
                     /locus_tag="WGR04_000132"
     CDS             complement(43985..44263)
                     /gene="rpsS"
                     /locus_tag="WGR04_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ0793773.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            complement(44270..45103)
                     /gene="rplB"
                     /locus_tag="WGR04_000133"
     CDS             complement(44270..45103)
                     /gene="rplB"
                     /locus_tag="WGR04_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ0793774.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     SNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQVGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            complement(45109..45423)
                     /gene="rplW"
                     /locus_tag="WGR04_000134"
     CDS             complement(45109..45423)
                     /gene="rplW"
                     /locus_tag="WGR04_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ0793775.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            complement(45420..46040)
                     /gene="rplD"
                     /locus_tag="WGR04_000135"
     CDS             complement(45420..46040)
                     /gene="rplD"
                     /locus_tag="WGR04_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ0793776.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            complement(46040..46684)
                     /gene="rplC"
                     /locus_tag="WGR04_000136"
     CDS             complement(46040..46684)
                     /gene="rplC"
                     /locus_tag="WGR04_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ0793777.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGISKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
BASE COUNT        12855 a        12211 c        10644 g        11309 t
ORIGIN      
        1 gctctatcaa aggggcgcat taattttctt aacattcccc tttgacagcc aagtgaaagg
       61 ggctttttta tgtcagcagt aaatgtaata ttttcctgtt cttattggag aatatttaaa
      121 aaatcagatt cttgtgtttt gtgtttttat cagttcagac atggcgaacc gcataaactc
      181 attaatcaag agaatttttc aaagctttat caggcgttcg attatataga ttcggttggt
      241 tcgaattttc cagtgattat cacaacggat ggttgtggtc ttttttgttg atctttaaaa
      301 gtttgtcagg atttggcttt cggtcgttga ccgtcgtacg cgctttagcg cggaagacgg
      361 gaaacggctg aaagcccccc cccttgacta acaggggggg agcgaaatta aaaaccaatt
      421 ccaagagtag tgaacgaatg agtgaagttg aatatttctc acactttata tcggacggaa
      481 aagggaagct tttagaaatt ccgcagcgaa gaggtaagca agacggggtt tttgttgatt
      541 ggatttcatt cacattccat gaagatactt tactgaaagt ttccggttgc cctttatttt
      601 ctgatgctga atacatgtat gtattaagca gaaagctgga agaaattcta ggttttggca
      661 taacgcgcaa atgcaaatca aggggcaaca aattctatga atccatgtat aggttaggtt
      721 cggatgatgt tgattatgga gaggtgcatt tcggaggtca gcgcaatact gttttagttg
      781 agttgaaagg tactggttgc agcgttgcaa gtccgggttg ggagttgagg ctaaagcagt
      841 ttctcgatga ttcgataagg acaagaataa cgcgaattga cctagcactt gatttttttg
      901 atggagagta cacgccggat caggcgttgt tagatcacga taatggtttt tttgataaca
      961 gcaatcaaag gccgaaatct gaaatgatcg gtacggcttg gcggaatgag gacgggagcg
     1021 gcaagacatt ttatgtaggt cgcaagaaaa attctcgttt tgttcgtgtt tatgagaaag
     1081 gcaggcagct tggagataaa gaaagcaaat gggtaaggtt cgagatccag tttaattatg
     1141 gagatataga aatacccttg gatattttaa taaatcaggg ttcgtatttc tgtggagctt
     1201 ttccaatttg tagaaaattt aaaaatatgc cggttcccga aaggtttgat cagagaaaga
     1261 aaaagcttaa tttaactttc gagcataaat tgcattacgc gaaaaacgcg gttggaaaac
     1321 tggtcaattt catgattgaa atgggttttg ataatagcga aattgtggaa tctttaaagg
     1381 cagattcggg atttcccaaa ggattagaac ctgaaaaata tgctctggaa atgttaaggg
     1441 acggtttgaa acacggtttt attcatgaac agccggatat tgatttggaa attgaacttg
     1501 atgaattggg ggttattgct tttaaaaatt ctgacaaatt cgatagggaa aaaaggcttt
     1561 ttagtcctga ttatgatgtc gagaaagaaa ggaaatatca ggaatattta agtaaagttt
     1621 atcatcaaaa tgtagattat gattattttt aaaggaaatc aaaatgttta atcaaactca
     1681 aactgtaact tatcctgcaa cttttttggg agccaaaaaa ttcaaaggtg aaattgatgg
     1741 ctctaacatt gacacttgtt ccgtattggt tgtaacacct ttaccggcac agtcgggtaa
     1801 tgctgttgga tttacggcag cacaaatgaa gttcggggat agtaagaatt tctcaaggtt
     1861 agagaatctc aaatatccgt gcgaagttat ggtaatggtt gaaatgactt cgacaggtaa
     1921 aggcatggtt ccgtcattaa ttgattttca ggtggcggaa aagccgaaag gttgatttat
     1981 gaaatttgaa gaacgtttca tagttcaaga cttggaaacg catgacttta tttatcccga
     2041 tcctttcggt gatgtggggt ttactcaaaa tattaaatca gcaggtcaat ttgaaagcta
     2101 cgaagatgcg ttgaattcag gcataaatga aatgggcgga ggattccaga tatttcagtt
     2161 cttcgtaaaa tcggaataaa agaaaaacag gctcggcggg cggtctgtta acctttcaca
     2221 aagcccgcaa caaaggaaaa atatcatgaa aatgaacctt gcaacactaa ttatcggctg
     2281 ggtggtctgt atgtttcttt ttcttttcgc aatcctctat tttatcggct aaaaacgaga
     2341 ttcggaaaag acttcgtccg gatgaagcaa gtcaagaagt cgtcttattt taaatatcaa
     2401 aaaaaggaaa aaaacgatga acatcgttaa aaaatatgct gtaaaagcat tgttggcagc
     2461 tggtatcttc acaccggcta ttgttatggc agatggcttt gatgcatccg cgattggtac
     2521 gcaagtggcg aatgtaatca tgggttttgt ggcaatggtt tccgccgtgg gtatggcggc
     2581 cattacggtc atcttggcaa tccaaggctt caagatggct tggagcatga tcaaatctgt
     2641 caaataaaca gagtgaagaa aaaggggcgt ataaatgggc tatcgtgtcg gcataaattg
     2701 ttttgataca agattgcagg cagacgacta tttattgtcg tcccttcctc ctactgttac
     2761 ccaggacgga aaaatcatca ggccggaaag ggtgggcgat aaatggattt tgaacggaaa
     2821 gccggttacg ttgtcttatc cggaatgttc caattttgag cagataaagc aaggttctta
     2881 tgtcggttcg acggttctaa ttctgtttgt agtcatttac ggtttcaggc ttctgattaa
     2941 ttttttaaaa gacataggca aggttgggac tgattgatga ttatagattt ctggtttctt
     3001 ctcggtttct tcttggcttt gtctgttgct tggctgtttt ggtaacggtt ggtagaatcg
     3061 gctttttaga gtgttttaaa aggtccgaat tatgtttatt tctgaatatc atttagttaa
     3121 atttcaaact gattcacata tttatagaga tttaccacaa gcgttaattt attataggga
     3181 attgattaga aaaggggttt ttaaaacttc gttttcattt gatattttta ggaatttctt
     3241 tcatcgttat gatagagatt ttatagaaat tcaattccct gattcttcta cattattaat
     3301 taaattagat gaagcaaaat gttatgttta ttatcctagg gcgaaatttt ttaaagatta
     3361 tcctatgctt tagttttttt gtatctaaat ttgcattggc atcagtaaat gctccgggta
     3421 aatttgatag ggttgaagtt tatgatgatg gcagatattt aggtattcga ggttcagatg
     3481 acaaaagaag aagaatttgg aaaggtctat ttgatagaga atcgggaaga tatttaactt
     3541 cagaagctca agatttaaaa gttaggcatg tatctactgg agcatcaagt acgggtaaag
     3601 ttagttcggt tgtatcttca tcagtttccc gcgctggcgt attggcgggg gtcggcaaac
     3661 ttgcccgctt aggcgcgaaa ttcagcacaa gggcggttcc ctatgtcgga acagcccttt
     3721 tagcccacga cgtatacgaa actttcaaag aagacataca ggcacaaggc taccaatacg
     3781 accccgaaac cgacaaattt gtaaaaggct acgaatatag taattgcctt tggtacgaag
     3841 acgaaagacg tattaataga acctatggct gctacggcgt tgacagttcg attatgcgcc
     3901 ttatgtccga ttacagcaga ttccccgaag tcaaagaatt gatggaaagc caaatgtata
     3961 ggctggcacg tccgttttgg aattggcata aagaagaact gaataaatta agttctttgg
     4021 attggaataa ttttgtttta aatcgttgca catttgattg gaacggcgga gattgtgtgg
     4081 tcaataaagg tgatgattac agaaatgggg ctaatttttc ccttagccgc aatccgaaat
     4141 acaaagaaga aatggatgcc aaaaagctgg aagagatttt atcgttgaaa gtcgatgcca
     4201 atcccgacaa atacataaag gcaaccggat atcccggtta ttccgaaaaa gtagaagtag
     4261 cacccggaac aaaagtgaat atggggcctg ttacggacag gaacgggaat cccgttcagg
     4321 ttgtcgcaac attcggcagg gattcgcaag gcaacaccac agtggatgtt caagtaatcc
     4381 cgcgtcccga cctcacaccc ggaagcgcgg aagcaccgaa cgcacagccg ctgcccgaag
     4441 tatcgcccgc cgaaaacccc gcaaacaacc cgaaccccaa tgagaacccc ggcacgagcc
     4501 ccaatcccga acccgacccc gatttgaatc ccgatgcaaa tcccgatacg gacggacagc
     4561 ccggaacaag ccccgattcc ccggccgttc cggaccgccc aaacggcagg gacggcaaag
     4621 atggcgggct tttgtgcaaa ttcttccccg acattctcgc ttgcgacagg ctgcccgagc
     4681 ccaatccggc agaagattta aatctgccgt ctgaaaccgt caatgtagag tttaagaaat
     4741 ccggaatctt tcaagattcc gcacagtgtc ccgcccccgt tacgttcact ataaccgtgc
     4801 ttgattcaag caagcagttc gcgttcagct ttgagaacgc atgtaccata gccgaacggc
     4861 taaggtacat gcttctcgcc cttgcttggg cggttgccgc ctttttttgt atccgcacag
     4921 tatcccgtga agtctagcag gcgcagcacc gccgggcttc agtaacttgt gccaaggcag
     4981 ggggaggacg tccagaaaga tttgtaaaga cggctttatc gtctttataa atctttttgg
     5041 ataccccttg ccgccccgcc aaaagaacac actctgctgc aagggcaggt ggtaaggcgc
     5101 gcgctttttg cgccgtcccc ctgcccccgc agcgtcgcaa gtgagactgg gggtgtgggg
     5161 gctagtcccc gcaaaatctt tcagattaag aaacattttt ttaatgaggc aaccgtgcct
     5221 tttaagaaag ggatagcaaa tgaaattgtt ggccgcattg attccgctct tgatgagcgt
     5281 cgtaggccgt atattgactg cattgggatt gatggccgtg acctattcgg gggtggatag
     5341 attggtagcc cattttcaac aggcgataac ccatagcata acgggcgcac ctcaagcaat
     5401 gttacagctt ttctatataa gcggcggtgg tactgttcta aacattcttt tcggcgcgat
     5461 cgcctttatt ctgtcattca aacaaatgac aaaactagca acctcaatcg ggaagaaaaa
     5521 ataaatggca gagatctgtt tgataaccgg cacgcccggt tcagggaaaa cattaaaaat
     5581 ggtttccatg atggcaaacg atgaaatgtt taagccggat gaaaacggca tacgccgtaa
     5641 agtatttacg aacatcaaag gcttgaagat accgcacacc tacatagaaa cggacgcgaa
     5701 aaagctgccg aaatcgacag atgagcagct ttcggcgcat gatatgtacg aatggataaa
     5761 gaagcccgaa aatatcgggt ctattgtcat tgtagatgaa gctcaagacg tatggccggc
     5821 acgctcggca ggttcaaaaa tccctgaaaa tgtccaatgg ctgaatacgc acagacatca
     5881 gggcattgat atatttgttt tgactcaagg ctctaagctt ctagatcaaa atcttagaac
     5941 gcttgtacgg aaacattacc acatcgcttc aaacaagatg ggtatgcgta cgcttttaga
     6001 atggaaaata tgcgcggacg atcccgtaaa aatggcatca agcgcattct ccagtatcta
     6061 tacactggat aaaaaagttt atgacttgta cgaatcagcg gaagttcata ccgtaaataa
     6121 ggtcaagcgg tcaaagtggt tttacactct gccagtaata gtattgctga ttcccgtgtt
     6181 tgtcggcctg tcctataaaa tgttgagcag ttacggaaaa aaacaggaag aacccgcagc
     6241 acaagaatcg gcggcaacag aacagcaggc agtacttccg gataaaacag aaggcgagcc
     6301 ggtaaataac ggcaacctta ccgcagatat gtttgtcccg acattgacgg aaaaacccga
     6361 aagcaagccg atttataacg gtgtaaggca ggtaaaaacc tttgaatata tagcaggttg
     6421 tgtacaaggc ggaagaaccg gatgcacctg ctattcgcat caagggacag cattgaaaga
     6481 agtgacggag ttgatgtgca aggactatgt aaaaaacggc ttgccgttta acccatacaa
     6541 agaagaaagc caagggcagg aagttcggca aagcgcacag caacattcgg acagggcgca
     6601 agttgccacg ttgggaggaa agccgctgca aaatcttatg tatgataatt ggcaggagcg
     6661 aggaaaaccg tttgaaggaa tcggcggggg cgtggtcgga tcggcaaact gaagaaaacg
     6721 gcaagagaga aaaaagaccc gtaaaccgtt tgaatataga cggtttacgg gtctttgttt
     6781 cgcgcaaagc aaaggctaag gcagtcaggc agcaaatccc gcaatgtatc aaaacagacg
     6841 cgtagaaatg ccggctgcct ttatccatcc tcaaaattga atatcatcct agccgtatca
     6901 aggctgtata aataaggaaa ataccaatga atataatcgg gctggacatc tcaaaggaca
     6961 ccatagacgc aacattgcat aaaacaaacg gaagtatcca ttacattaaa tttaagaata
     7021 atgatgatgg attaaaacag tttagattgt ggataaaggg aaacagaatc agaaaagcct
     7081 atatcggcat ggaggcaaca ggcatctatt acgaaaaggc agcagatatg ctttcttcct
     7141 actatactgt ttacgttatt aatcccttaa aaatcaagga ctacggaaaa agcaggttta
     7201 accgtaccaa aaccgacaaa gcagattcaa acctgatagc agactacata aaaaggcatc
     7261 aagatacatt gataccgtat cagataccca aaaacaaagc actgcaaaaa ctgattaatc
     7321 ttaaaaacca attacagcaa cagcagaagc aaattaaaaa ccgtcttcat agcactgaag
     7381 aagacttcat aaggaacata catcaagact tgatagatac catacaggac aagatggaac
     7441 aggtaaaaat agccatatcc gaacaaatca aaaaacaaac ggactataac cattaccgca
     7501 atcttcaaac catcccgagc ataggcaaag acactgcatt agttctttat gcgcaactga
     7561 cagaaaaaca ttttaaaacc gcaaaccagt ttgtatccta tgccggatta agtcccgcca
     7621 tcatacaatc agggacaagc gtaagaggtc ggggcagatt gagccgatac ggaaacagac
     7681 gattaaaaag tacgctgtat atgcccgccc tttgtgctta ccgttttaac gcatttccga
     7741 aattaataaa taatctgaaa aaagcgggta agccaaagat ggtaattatc gttgccatca
     7801 tgcgcaaact ggcgaagctc gcctattata ttgttaaaac cggccagcct tacgatgcgg
     7861 aaagacaccg attgaatcaa taaaattcaa caaaattaaa cggttacgcg aatatatttg
     7921 tgtaaccgtg catttgcata tcgtaaataa acgtaaataa aaataacaat ataaatcagt
     7981 atattgcaac tttgtttttt attttgtgtt gacgggcaac atatcatctg cgcgggaatg
     8041 acgatatttc agttttgtgt ttttgatttt tttgtttttg cgggaatgac gatatagtgg
     8101 attaacaaaa atcaggacaa ggcgacgaag ccgcagacag tacagatagt acggaaccga
     8161 ttcacttggt gcttcagcac cttagagaat cgttctcttt gagctaaggc gaggcaacgc
     8221 cgtactggtt tttgttaatc cactataaat gacgatataa gtatttttat tttaatccgc
     8281 catattaacg cactcggcca aacagcataa aggcacgggc agcccgattt ggattgacct
     8341 atttccccga caccctcgcc atcagctcgt ccgccaaggc aagatacgcc ttggtaccct
     8401 ttgcctgcgc gtcgtaagcc ataaccggca taccgtggct cggggcttcc gcaagacgga
     8461 tattgcgcgg gatggcggtt tcaaaaagca aatccccgaa atggctgcgc aactgttcgc
     8521 tgacttcggc aaccagcctg ctgcggctgt cgtacatcgt gcgcacgatg cccgtgatgt
     8581 ccaaatcggg attgaccgcc tgacggattt tgcgcacggt tgcaatcaaa tcggaaatcc
     8641 cttccagcgc gtaatattcg cacaacatcg gtacaatcac gccgcccgcc gccaccaagc
     8701 cgttaagcgt caacagcgtc agcgaaggcg gacagtcgat caggataaag tcgtaatctt
     8761 ccgccactgc cttgagtgcg tttttcaaac gcacttcccg ggcgatttcc tgcaccagct
     8821 cgatttccgc accggccagc gcgcggttcg cacccaacac agcgtatccg ccctctttgc
     8881 tgcgtaccgt cgccgatttc acgtccgcat cgcccaataa gacctgataa acgccggact
     8941 gcaaacccgc cttgtcgata ccgctgcccg tcgtcgcatt gccctgcgga tccaaatcaa
     9001 ccaccagcac gcgtttgccg cgcgatgcca gcgaagccgc caaatttacc gtcgtcgtcg
     9061 ttttgcccac accgcccttc tgattggcga tggcaaggat gttcgcactc atgtcccgcc
     9121 cgtgtcccgt ctgatgttga aaaaagaaaa acagtgcgga attttacctt tatccgcaca
     9181 aaaaaggtat tcatgccgtc tgaagcgcgc tcaacgcttg ctcaggataa cgatatggcg
     9241 ttccgcatcc aagcccggca cgtcgagcct ttggactttt tcgacgcaca catcctgcgg
     9301 caggcggtca atttcttcct gcggatacac gcccttcatc gccgcccagt agccgccgtc
     9361 tttcaacaga tgcgccgtcc aagacacaaa atccgccagt tctgcaaacg cacggctggt
     9421 aaccacgtcg gcacgcacgt ccgaaaccgc ctccacgcgt ccggatacca cgcgcacatt
     9481 gtccaacccc aactcgataa ccgcctgctg taaaaaagcc gttttcttcg tattcgcatc
     9541 caaaagggtt atttgcacat ccggacggca caccgccgcc ggaatgccgg gctgaccgcc
     9601 gcccgaaccg acatccagca tcgtttgcac accctcgata tggggcagca gcgtcaggct
     9661 gtccaaaaga tgatggacaa tcattttttc ctcgtcgcgc agggcggtca gattgtaggt
     9721 tttgttccac tttttcaaca aatccacata ggccaaaagc ctgtcctgcg ccgctgcgga
     9781 aatatccaaa cccaatgcgg cgatgcctgc acgcaggcgt tctttgcgtt ccatatccct
     9841 atcctgtcca tgttcaggcg taatgttaac ggaaaaacat gccgtctgaa accgcgtgtc
     9901 ggggttcaga cggcatttcc cgttccaacc tattttttcc tgtgaaacac caccggctgc
     9961 accggcgcgc gcgtcaggcg tttgggcagc gtcagcatcg ccaccagcca cacggcaaac
    10021 gcatattcca acaccgcctc cccacgcagc ccgcccaaag ccccactgcc aagcagcagc
    10081 tcgaaaatcc aagccaccgc tgccgccgcg caagccgcca cgcccgacag aaacagtgtg
    10141 taacgcgaat gccgccgcat catctttttg tacaccagtt tcgtcagacc cgccgacaca
    10201 accacattca aaaacgcaaa cacaccgaac acaaaccaca ccacgccgtc ccagcctcct
    10261 cgccagccga atccgccgtc cgacaccgac accagcaacc ccgtccgtcc gaacgcaagc
    10321 gcaaaggcat catgcaccgc caacgcaaac ggcagcgtca aaaacaccag tcccgcaaac
    10381 agggccgcat aaggatcttt ctcgttcaca aacatcgccg tccccctctt ccgaagcaga
    10441 ccgcattata ccgcgcactg ccttgccgcc cgcccgaaaa gttgcacaaa caaccgttca
    10501 tatatatcat aacgaaaaac gccggtgtag ctcagtcggt agagcagcgc attcgtaacg
    10561 cgaaggtcgg gggttcgatt cccttctccg gcaccaatac caagcacaga ccctcccttc
    10621 ctcgggaagc ctgtgctttt tcacatttcc gcttcagacg gcacaaccga tatgaacacc
    10681 tcgcaacgca accgcctcgt cagccgctgg ctcaactcct acgaacgcta ccgctaccgc
    10741 cgcctcatcc acgccgtccg gctcggcggg gccgtcctgt tcgccaccgc ctccgcccgg
    10801 ctgctccacc tccaacacgg cgagtggata gggatgaccg tcttcgtcgt cctcggcatg
    10861 ctccagtttc aaggggcgat ttactccaag gcggtggaac gtatgctcgg cacggtcatc
    10921 gggctgggcg cgggtttggg cgttttatgg ctgaaccagc attatttcca cggcaacctc
    10981 ctcttctacc tcaccgtcgg cacggcaagc gcactagccg gctgggcggc ggtcggcaaa
    11041 aacggctacg tccctatgct ggcggggctg acgatgtgca tgctcatcgg cgacaacggc
    11101 agcgaatggt tcgacagcgg cctgatgcgc gcgatgaacg tcctcatcgg cgccgccatc
    11161 gccatcgccg ccgccaaact gctgccgctg aaatccacac tgatgtggcg tttcatgctt
    11221 gccgacaacc tgaccgactg cagcaaaatg attgccgaaa tcagcaacgg caggcgcatg
    11281 acccgcgaac gcctcgaaga gaacatggcg aaaatgcgcc aaatcaacgc acgcatggtc
    11341 aaaagccgca gccacctcgc cgccacatcg ggagaaagcc gcatcagccc cgccatgatg
    11401 gaagcgatgc agcacgccca ccgtaaaatc gtcaacacca ccgagctgct cctgaccacc
    11461 gccgccaagc tgcaagcccc gaaactcaac agcggcgaaa tccggctgct cgaccgccac
    11521 ttcacccaac tccaaaccga cctgcaacaa accgtcgccc tcatcaacgg caggcacgca
    11581 cgccgcatcc gcatcgacac cgccatcaac cccgaactgg aagccctcgc cgaacacctc
    11641 cactaccaat ggcagggctt cctctggctc agcaccaata tgcgtcagga aatttctgcc
    11701 ctcgtcatcc tgctgcaacg cacccgccgc aaatggctgg atgcccacga acgccaacac
    11761 ctgcgccaaa gcctgcttga aacacgggaa cacagttgac ggtcaaacac gatgccgtct
    11821 gaaacgcctg ccgcttcaga cggcatctcc aatcctcctg ccttgtcgtc atgcccgaat
    11881 aacggtacta ttcgcagtta acagttttca tgccgtctga aaggtttgaa gcgcgtttca
    11941 gacggctgcc cgcctacctt atcttaccga aagaacaata tgatcaacga tattcaaaaa
    12001 acagccgaag gcaagatgca gcgttcggtc gaagtactga aagaaaatct ggcgaaagtg
    12061 cgtaccggcc gcgcgcatac cggcctgctc gaccaagtgg aagtcgaata ctggggcagc
    12121 atggtccccg tcagccaggt tgccaacgta acgcttctgg acgcgcgcac catcggcgtg
    12181 aaaccgtttg agggcaatat ggcggccaaa gtcgagaaag ccatccgcga ttcaaacttg
    12241 ggactgaacc cggcagctgt cggcgacctg atccgcgtgc cgatgcccat gctgaccgag
    12301 gaacgccgca aagacctgat taaagtcgta cgcggcgaag cggaagaagg ccgcgtctct
    12361 atccgcaacg tgcgccgcga tgccaacgac cacatcaaaa aactcctcaa agacaaagaa
    12421 atttccgaag acgaggcacg tcgcggcgaa gaagcggttc aaaaactgac cgacaaatac
    12481 attaccgaag ccgacaaact cctgactgcc aaagaagaag atttgatggc aatttaacct
    12541 gcacggttcg gcgttcagac ggcatttgaa cgccgaaccg cgaaaggcag acatgaaaag
    12601 cagcacgcag gccgttttag aacacaccgc cattcccaag catatcgccg taattatgga
    12661 cggcaacggc cgttgggcga aaaaacgttt cctcccgcgc ataatgggac acaaacgcgg
    12721 tttggacgca ttggaaaata tggtgaagca ttgcgccaaa ctgggtgtgc aatatctgac
    12781 cgtgtttgcc ttttcaaccg aaaactggcg ccgccccgaa gacgaagttt cgttcctgat
    12841 ggggctgttt ttacaggctt tgcaaaaaca ggtacgccgc ctgcacgaaa acaatatgcg
    12901 cctgaagata ttgggcagcc gcgaacgctt caaccggcag attctgcaag gcatcgaaga
    12961 agcggaggcg ttgacggcaa acaataccgg tctgaccctg agcattgccg ccgattacgg
    13021 cggacgttgg gatattttgc aggcggcaaa caaactgatt gccgaaggcg tatccgagat
    13081 tacggaagac acgctggcca aacacttgat gctgggcgat gcaccggaac cggatttgtt
    13141 catccgcacc ggcggagaaa cgcgcatcag caatttcctg ctctggcaga tggcatatgc
    13201 agaactgtat ttcaccgaca tcctgtggcc cgatttcgac gaaaccgcct tagatgccgc
    13261 cgtcgcttcg ttccaaaaac gcgaacggcg gttcggacgc acctccgagc aactgcctat
    13321 cgaacagcaa aggaactgaa tatgctgaaa caacgggtga tgaccgctat gtggctgctg
    13381 ccgctgatgc tgggcatgct gttttacgcg ccgcaatggt tgtgggctgc attttgcgga
    13441 ctgattgccc tgattgcctt gtgggaatat gcccgtatgg gcggtttgtg caaaattaaa
    13501 accaaccatt acctcgccgc aaccttggtt ttcggcgtgg ttgcctatgc gggcggctgg
    13561 atgctgccta atttggtttg gtatgttgtt ttggcatttt ggctcgccgt tatgccttta
    13621 tggttgagat tcaaatggag gctcaacggc ggttggcagg tttatgccgt cggctggctt
    13681 ctggtcatgc cgttttggtt cgcgctcgta tccctgcgcc cgcatcccga tgatgccctg
    13741 ccgctgctcg ccgtgatggg tttggtgtgg gttgccgata tttgcgcgta tttcagcggc
    13801 aaggcgttcg gcaaacacaa aatcgcaccg gcaatcagcc ccggcaaaag ctgggaaggt
    13861 gcaatcggcg gcgcggtttg cgtggcagtg tacatgaccg ccgtacgaag tgccggctgg
    13921 ctggcattcg atacaggctg gttcgatacc gtgttaatcg gtttggtgct gaccgttgtc
    13981 agcgtatgcg gcgacctgtt ggaaagctgg ctcaagcgcg cggcgggcat taaagacagc
    14041 agcaacctgc tgtccggaca cggcggcgtg ttcgaccgta ccgacagcct gattgccgtt
    14101 atcagcgtct atgcggcgat gatgtcggtt ttaaattgat tctatgccgt ctgaaaacgc
    14161 ttcagacggc atccggtata aagttatcct cattatgaca ccacaagtcc tgaccatatt
    14221 aggcagtacc ggcagcatag gcgaaagcac gctggacgtt gtctcccgcc accccgaaaa
    14281 attccgcgta ttcgcgctgg cagggcataa gcaggtcgag aaattggcgg ctcaatgtca
    14341 aacgttccgc cccgaatatg ccgtcgttgc cgatgcggaa cacgccgctc ggcttgaagc
    14401 cctgttgaaa cgcgacggca cggcgacgca ggttttacac ggcgcgcagg cattggttga
    14461 cgttgcgtct gccgacgaag tcagcggtgt catgtgcgcc atcgtcgggg cagtggggct
    14521 gccttccgcg ctcgcagcgg cgcaaaaagg caaaaccatt tatctggcga acaaagagac
    14581 gctggtggtt tccggcgcgt tgtttatgga aaccgcccgt gcaaacggcg cggcagtgct
    14641 gcccgtcgac agcgaacaca acgccgtttt ccaagttttg ccgcgcgatt acgccggccg
    14701 tctgaacgaa cacggcatcg cttcgattat cctgaccgct tccggcggcc cgtttctgac
    14761 cgccgattta aacacgttcg acagcattac gcccgcccaa gcggtcaaac accccaattg
    14821 gagcatgggg cgcaaaatct ccgtcgattc cgccaccatg atgaacaaag gtttggagct
    14881 gattgaagcg cattggctgt tcaactgtcc gcctgacaag ctcgaagtcg tcatccatcc
    14941 gcaatccgtg atacacagca tggtgcgcta ccgcgacggc tccgtgttgg cacaactggg
    15001 caatcccgat atgcgaacgc ccatcgccta ttgtttgggc ttgcccgagc gcatcgattc
    15061 gggtgtcggc gacctggatt tcgacgcatt gtccgcgctg accttccaaa agcccgactt
    15121 tgaccgcttc ccctgcctga agctcgccta tgaagccatg aacgcaggcg gagccgcgcc
    15181 ctgcgtattg aacgccgcca acgaagccgc cgtcgccgcc tttttggacg gacagattaa
    15241 gtttaccgac attgccaaaa ccgtcgccca ttgtctcgca caagactttt cagacggcat
    15301 aggcgatata ggggggctct tggcgcaaga tgcccggaca cgcgcacaag cgcgggcatt
    15361 tatcggcaca ctgcgctgat gccgtctgaa caccgttatc aaaggaaaac catttgcaca
    15421 cccttctagc ttttatcttc gccatcctga ttttggtcag tctgcacgaa ttcggacact
    15481 acgccgttgc aaaattatgc ggcgtaaaag tgctgcgttt ttccgtcggc ttcggcaaac
    15541 cgttttttac ccgaaagcgc ggcgataccg aatggtgttt ggcacccatc cccttgggcg
    15601 gctatgtcaa aatggtcgat acgcgcgaag gcgaagtatc agaagccgat ttaccctacg
    15661 cttttgacaa acaacacccc gccaaacgca tcgccatcgt cgccgccggc ccactgacca
    15721 acctcgcact ggcggttttg ctgtacggac tgagcttttc cttcggcgta accgaactgc
    15781 gcccctacgt cggcacagtc gaacctgaca ccattgccgc ccgcgccggc ttccaaagcg
    15841 gcgacaaaat acaatccgtc aacggcacac ccgttgcaga ttggggcagc gcgcaaaccg
    15901 aaatcgtcct caacctcgaa gccggcaaag tcgccgtcgg cgttcagacg gcatcgggcg
    15961 cgcaaaccgt ccgtaccatc gatgccgcag gcacgccgga agccggtaaa atcgcaaaaa
    16021 accaaggcta catcggactg atgcccttta aaatcacaac cgttgccggc ggcgtggaaa
    16081 aaggcagccc cgccgaaaaa gcaggcctga aaccgggcga caggctgact gccgccgacg
    16141 gcaaacccat cgcctcatgg caggaatggg caaacctgac ccgccaaagc cccggcaaaa
    16201 aaatcaccct gacctacgaa cgcgccggac aaacccatac cgccgacatc cgccccgata
    16261 ctgtcgaaca gtccgaccac accctgatcg ggcgcgtcgg cctccgtccg cagccggaca
    16321 gggcgtggga cgcgcaaatc cgccgcagct accgtccgtc tgttgtccgc gcattcggca
    16381 tgggctggga aaaaaccgtt tcccactcgt ggacaaccct caaatttttc ggcaaactaa
    16441 tcagcggcaa cgcctccgtc agccatattt ccgggccgct gaccattgcc gacattgccg
    16501 gacagtccgc cgaactcggc ttgcaaagtt atttggaatt tttggcactg gtcagcatca
    16561 gcctcggcgt gctgaacctg ctgcccgtcc ccgttttgga cggcgggcac ctcgtgtttt
    16621 atactgccga atggatacgc ggcaaacctt tgggcgaacg cgtccaaaac atcggtttgc
    16681 gcttcgggct cgccctgatg atgctgatga tggcggtcgc cttcttcaac gacgttaccc
    16741 ggctgctcgg ttagatttta cgtttcggaa tgccgtctga aaccgcattc cgcaccacaa
    16801 ggaactgaca atgaaactga aacagattgc ttccgcactg atggtcttgg gcatatcgcc
    16861 tttggcactt gccgacttca ccatccaaga catccgcgtc gaaggcttgc agcgtaccga
    16921 gccgagtacc gtattcaact acctgcccgt caaagtcggc gacacctaca acgacacaca
    16981 cggcagtgcc atcatcaaaa gcctgtacgc caccggtttc tttgacgacg tacgcgtcga
    17041 aactgcggac gggcagctcc tgctgaccgt tatcgaacgc cccaccatcg gctcgctcaa
    17101 catcaccggc gcaaaaatgc tgcaaaacga cgccattaag aaaaacctcg aatcgttcgg
    17161 gctggcgcag tcgcaatact ttaatcaggc gacactcaat caggcagtcg ccggcctgaa
    17221 agaagaatac ctcgggcgcg gcaaactcaa tatccaaatc acgcccaaag taaccaaact
    17281 cgcccgcaac cgcgtcgaca tcgacatcac gattgacgag ggcaaatccg ccaaaatcac
    17341 cgacatcgaa tttgaaggca accaagtcta ttccgaccgc aaactgatgc ggcagatgtc
    17401 gctgaccgaa ggcggcattt ggacatggct gacacgaagc aaccaattca acgagcagaa
    17461 atttgcccaa gacatggaaa aagtaaccga cttctaccag aacaacggct acttcgattt
    17521 ccgcatcctc gataccgaca tccaaaccaa cgaagacaaa accaagcaga ccatcaaaat
    17581 caccgtccac gaaggcggac gtttccgttg gggcaaagtc tccatcgaag gcgacaccaa
    17641 cgaagtcccc aaagccgaac tggaaaaact gctgaccatg aagcccggca aatggtacga
    17701 acgccagcag atgaccgccg ttttgggtga gattcagaac cgcatgggct cggcaggcta
    17761 cgcatacagc gaaatcagcg tacagccgct gccaaacgcc gaaaccaaaa ccgtcgattt
    17821 cgtcctgcac atcgaaccgg gccggaaaat ctacgtcaac gaaatccaca tcaccggcaa
    17881 caacaaaacc cgcgacgaag tcgtgcgccg cgaattgcgc caaatggaat ccgcgcctta
    17941 cgacacctcc aagctgcaac gctccaaaga gcgcgtcgag cttttgggct acttcgacaa
    18001 cgtacagttt gatgccgtcc cgcttgccgg cacacccgac aaagtcgatt tgaacatgag
    18061 cctgaccgaa cgttccaccg gctcgctcga cttgagcgcg ggctgggtac aggataccgg
    18121 cctggtcatg tccgcaggcg tttcccaaga caacctgttc ggtacgggca agtcggccgc
    18181 cctgcgcgcc tcacgaagca aaaccacgct caacggctcg ctgtcgttta ccgacccgta
    18241 cttcacggca gacggggtca gcctgggcta cgatgtttac ggaaaagcct tcgacccgcg
    18301 caaagcatcg accagcatca aacaatataa aaccaccacg gcaggcgcag gcatccgcat
    18361 gagcgtgcct gttaccgaat acaaccgcgt gaatttcggt ttggtggcag aacacctgac
    18421 cgtcaacacc tacaacaaag cgcccaaaca ctatgccgac tttatcaaac aatacggcaa
    18481 aaccgacggc acagacggca gcttcaaagg ctggctgtac aaaggtaccg tcggctgggg
    18541 gcgcaacaaa accgacagcg cgttatggcc gacgcgcggc tacctgacgg gcgtgaacgc
    18601 cgaaatcgcc ctgcccggca gcaaactgca atactactcc gccacccaca accaaacctg
    18661 gttcttcccc ttaagcaaaa ccttcacact gatgctgggc ggcgaagtcg gcattgcggg
    18721 cggctacggc aaaaccaaag aaatcccctt ctttgaaaac ttctacggcg gcggcctggg
    18781 ttcggtgcgc ggatacgaaa gcggcacgct cggtccgaaa gtgtatgacg aatacggcga
    18841 aaaaatcagc tacggcggca acaaaaaagc caacgtctcc gccgagctgc tcttcccgat
    18901 gcccggcgcg aaagacgcgc gcaccgtccg cctgagcctg tttgccgacg caggcagcgt
    18961 gtgggacggc aaaacctacg acgacaacag cagttccgcg accggcggca gggttcaaaa
    19021 catttacggc gccggcaata cccataaatc cacctttacc aacgaattgc gctattccgc
    19081 cggcggcgcg gttacctggc tctcgccttt aggcccgatg aaattcagct acgcctaccc
    19141 gctgaagaaa aaaccggaag acgaaatcca acgcttccaa ttccaactcg gcacgacgtt
    19201 ctaatcccgc aaatgccgtc tgaagccctt cagacggcat ttcgcggcaa cattcgaagg
    19261 agttttacca tgacccgttt gacccgcgcg tttgccgcgg ctctgatcgg tttgtgctgc
    19321 accgcaggcg cgcacgccga caccttccaa aaaatcggct ttatcaacac cgagcgcatc
    19381 tacctcgaat ccaagcaggc gcgcaagatt caaaaaacgc tggacagcga attttccgct
    19441 cgtcaggacg aattgcaaaa actgcaacgc gaaggtctgg atttggaaag gcagcttgcc
    19501 gaaggcaaac tcagaaacgc aaaaaaggcg caagccgaag aaaaatggcg cgggctggtc
    19561 gcagcgttcc gcaaaaaaca ggcgcagttt gaagaagact acaacctccg ccgcaacgaa
    19621 gagtttgcct ccctccagca aaacgccaac cgcgtcatcg tcaaaatcgc caaacaggaa
    19681 ggttacgatg tcattttgca gaacgtgatt tacgtcaaca cccaatacga cgttaccgac
    19741 agcgtcatta aagaaatgaa cgcccgctga ccctttcaga cggcataccg aacaggaaaa
    19801 ccatgattcc ggccacctac accctgtccc aaatcaccgc gcggctcggc ggcgaatggc
    19861 gcggcgagga catttccgtt accgccgtgc gcccgctcgc agacgcgcag gcggaacaca
    19921 tcagcttcct tgccaatccg aaatacaaag ccgaagtcca cgacagcagc gcgggcgcgg
    19981 tcatcgtttc cgccaaagcg gcagacggat ttgaagggcg caacctgatt gtcgccgacg
    20041 acccctatct ctatttcgcc aaagtcgccc gcctgttttc acccgtcgtc aaagcgcgcg
    20101 gcggcatcca tccgaccgcc gtcgtcgaac cgggcgcgac cgttcccgcc agctgcgaaa
    20161 tcggcgcgaa cgtctacatc ggcgcaaaca ccgtgctcgg cgaaggctgc cgcatcttgg
    20221 caaacgccgt cgtccaacac gattgcaaac tgggcgacga agtcgtcctg catcccaacg
    20281 ccgtcgttta ttacggctgc acactgggca gacgcgtcga aatccacagc ggcgcggtca
    20341 tcggcgcgga cggtttcgga ctcgcttttg ccggcgattc atggtttaaa atcccgcaaa
    20401 ccggcgcggt aacgctgggc gacgacgtag aaatcggctc gaacaccaac atcgaccgcg
    20461 gcgcgatgag cgacaccacc gtcggcaacg gcaccaaaat cgacaaccaa gtccaaatcg
    20521 gacacaactg caaaatcggt tcgcacaccg tcatcgccgc caaaaccggc atctcaggca
    20581 gcgtaaccat aggcagctac tgcatcatcg gcggcggcgt cggtacggtc ggacacatcg
    20641 aaatcgccga caaaaccacc atcggcggcg gcacgtccgt cacccacagc attaccgaaa
    20701 gcggcaaaca cctcgccggc atcttcccga tgtccaccca taaagaatgg gcgcgcaacg
    20761 ctgtttacat ccaccgctta agcgaaatga acaaacggct caaaacacta gagcagcagc
    20821 tttcagatgc cggtcaagac agcaaataac caaaccgact ttattcaagg aatacgacag
    20881 acatggacgt tcaactcccc atcgaagcca aagacatcca aaaactcatc ccccaccgct
    20941 atccgtttct ccagctcgac cgcatcaccg ccttcgagcc gatgaaaacc ctgaccgcga
    21001 ttaaaaacgt caccataaac gaaccccagt tccaaggcca ttttcccgac ctgcccgtga
    21061 tgcccggcgt actcatcatc gaagcgatgg cgcaggcgtg cggcacgttg gcgattttga
    21121 gcgaaggcgg gcgcaaagaa aacgaattct tcttcttcgc cggcatagac gaagcccgtt
    21181 tcaaacgcca agtcatcccc ggcgaccaac tcgtctttga agtcgagctg ctgaccagtc
    21241 ggcgcggcat cggcaaattc aacgccgtcg ccaaagtgga cgggcaagtt gccgtcgaag
    21301 ccgtgattat gtgcgccaaa cgcgtggttt gagtgttcag aaaaaggtcg tctgaaagtt
    21361 tcagacgacc taataaaaag aaaacccacc ggtttagatt tattcaaacc cagggacacg
    21421 gtggggatta gttttaccta actgtatcaa atatagtttg aaacgtcaat ttaaaaataa
    21481 agaatcaggt gttagcgtgg attataagga atgctgtagt attttttctg atttcagaat
    21541 ggaacggtat aaaaatgccg ttggggaaga caaagcagct gaattgtatt tattgaattt
    21601 atctttatca agagaacttt ttcatgttgt ttcaatattt gagattgttt taagaaacaa
    21661 aattgacatt tgccttcagc aggcgtttaa agacagaaac tggttatata acagcataca
    21721 gccacagacg aatcccgcat taaaatatca aggttgcttt ttaagaaatg gcacgaaaga
    21781 atctgctgaa ttaatcaagg ttgcattatc taaaatacaa aataatagcg ggggaaagtt
    21841 tgaccacaac caattggttg caggactagg attcggtttt tggcggtatc tgtttgcagg
    21901 agggaaagat gcccaatttg atgctacagg gaaagtattg atgaaagttt ttccaaaaaa
    21961 gcccaaatct acaccaagtg tgcaatataa ccaaaaatgg atttttcgag aactttccaa
    22021 tattaacaaa ttccgtaatc gtttggcaca tcacgagccg atttgtttta aaggtgcaat
    22081 aaaagatacc aactacgcta gaaatattca ccaatctatt tttgaactgc ttaattacat
    22141 ggatgtagat actgcttcag tatttagcca ttttagcgat caagtgattg cggtctgcga
    22201 tgaaatcgac aaactgtaat tttctattat ttgtcttaac aagcagcgga gtattcaaaa
    22261 catgaccctc atccacccga ccgccgtcat cgaccccaaa gccgaactcg actccggcgt
    22321 caaagtcggc gcgtacactg ttatcggccc caacgtccaa atcggcgcga ataccgaaat
    22381 cggtccgcac gccgtcatca acggccacac cagcatcggc gaaaacaacc gcattttcca
    22441 atttgccagc ctcggcgaaa ttccgcagga caaaaaatac cgcgacgagc cgaccaagct
    22501 gattatcggc aacggcaaca ccatccgcga attcaccacc tttaatttag gcacggtaac
    22561 cggcatcggc gaaacccgta tcggcgacga caactggatt atggcgtact gccacctcgc
    22621 gcacgactgc gtggtcggca accacaccat cttcgccaac aacacctcgc ttgccggaca
    22681 cgttaccgtc ggcgactacg tcgttttggg cggctacacg ctggttttcc aattctgccg
    22741 catcggcgac tacgctatga ccgccttcgc cgcaggcgta cacaaagacg taccgcccta
    22801 ctttatggca tcgggctacc gcgccgaacc ggcggggctc aacagcgaag gcatgcgccg
    22861 caacggtttt accgcagaac agatttccgc cgtcaaagac gtgtacaaaa ccctctacca
    22921 tcgcggcatt ccgtttgaag aggccaaggc agacatcctc cgccgcgccg aaacccaagc
    22981 cgagcttgcc gtattccgag actttttcac acaatcgacg cgcggcatca tccgctgacc
    23041 gtaccctttt gatgccgtct gaaaccccga aagcgtttca gacggcatcg gtttttttaa
    23101 agcggtatat tgtttgatcg atgcatcatt ttgtagattt cagaagtatg gcagtcggca
    23161 gcattgatga tttctgccgc ccgccacatc cctattcgga catttcattg tgacctccgc
    23221 cgctgctttt aaagaattgc aaacaatttc cgcagcttga accgacctgc tttcggagaa
    23281 aaaaccaaaa gtatttttca aaagaacata ttgtcttatt gaatattctg tgctgaaatt
    23341 acaggacact acaaaagtgg tgttttaaaa ataaaacaga caaaggagaa agccatgcaa
    23401 ttgtttttag acaatctcaa agcctttttc gaaaccgtca gcggctgggt ctggggatct
    23461 gttatgttga tgctgctggt cggcaccggc attgtgctga ccgttatgct gaaaggctta
    23521 cagttcacta tgttgggcta tgcgctgaaa caggcgtttg tgccgtcaaa gaagtatgaa
    23581 ggcggcgaag gccacgaagg cgatatttcc cattttgcgg cgttgatgac cgcgctgtcc
    23641 gccaccatcg gtacgggtaa catcgccggc gtggcgactg cggtggtaac cggcggcccg
    23701 ggcgcggtat tttggatgtg gatgaccgcc attttcggca tggccaccaa atacggcgaa
    23761 ggcgtgttgg cggtgaaata ccgcgtcaac aattccaaag gcgaaatgtc cggcggcccg
    23821 atgtattaca tcgaaaaagg cttgggcaaa aactggaaat ggatggccgt tgcatttgcg
    23881 ctgttcggca cattcgcttc cttcggtatc ggcagctcgg tgcagtccaa ctcggttgca
    23941 caggcggtgc aaaccagctt cggtatcgaa cctgcctata ccggcattac gttgaccgtt
    24001 ctgactgccg ttgtcgtttt aggtggtatt aaaggcatcg ccaaagccgc ttctttcatc
    24061 gtgcctgcta tggcggtgtt ttatgtgttg ggcggtcttt ccattatcgc gattaattcc
    24121 gatgcactga tgcctgccgt caagctaatt ttctccgatg cgttcagcgc gcaggcagtg
    24181 gcgggcggcg ctatcggtac ggtcatccgc tacggcgtgg cgcgcggcgt gttctccaat
    24241 gaggcgggta tgggttctgc gcctattgcc gccgcagccg cgaaaaccga ccatcccgtc
    24301 cgtcaggcct tggtttctat gaccggtacg tttttggata ccatcgtcgt ttgctcgatt
    24361 accggcatcg tgttggtaat gggactgctc ggcgcgggcg gcgagtttgt caaacctgaa
    24421 gtgagcggcg cggcactgac aaccgtcaca ttccaaaaaa tgctgcccgg catcggcggc
    24481 tggattgtca ccatcggcct gattttcttt gcctactcaa ccattctcgg ctggtgctat
    24541 tacggcgaaa aatgcgcggt gtatgttttc ggcgagaaat ttgccggttt gtaccgcgtc
    24601 ggttatgttt cttccgttat gctgggtacc gtgttgagcc ttgatttggt gtggctggct
    24661 tcggacacat tcaacggctt gatggcattg cccaacctga ttgcactttt attgatggcg
    24721 aaagtcatcg tcaatgaaac gcgcgacttc aaacaaaaaa tcacaaacgg cgaattgccg
    24781 cattaatatc cgaggccgtc tgaatccgaa tattcagacg gcacggcata ttaagtctgt
    24841 tttgaaaaag ccggagggag aaacatgaaa gtgcttgttt taggtgcggg tgttgccggc
    24901 gtgtcttccg cgtggtatct ggcagaggca ggacatgaag taacggtcat cgaccgcgcc
    24961 gagggtgtgg cgatggaaac cagttttgcc aacgcaggcc agctttctta cggctatacc
    25021 acgccttggg ctgcacccgg tatcccgacc aaagcgctga aatggctgtt taaaagccat
    25081 ccgcctttgc tgtttcgccc cgacggcagc ctgtatcaaa tcgaatggct gtggcaaatg
    25141 ctgcaaaact gcacggcagc gcactatcaa accaataaag agcgcatggt caggatttcc
    25201 gaatatagcc gtgaaatgtt ccgccgtttt gaagcgcaaa ccggcatgaa ttttgaggaa
    25261 cgcaaaaaag ggacgttgca gattttccgc caaaccaaag aagtcgaagc ggcagaacaa
    25321 gacattgccg ttttggaacg ctacggcgtg ccgtaccgcc gtctgaaacc cgaagaatgt
    25381 gcagaatttg agcctgcgct ggcacgcgtt accgccaaaa ttgccggcgg cctgcacctg
    25441 cctgcagatg cgaccggcga ctgccacctc ttcactgaaa acctatacaa attgtgtcag
    25501 gaaaagggcg tacagtttca tttcaaccaa accatcagcc gcatcgacca taacgggctg
    25561 cgcatcaaag ccgttgaaac cgaaacaggg cggtttgaag cagatgccgt tgtctgtgcg
    25621 ctcggctgct tcagtaggac tgtgttggcg cagttagacc tcgatctgcc gatttatccc
    25681 gtcaaaggct attccttgac cctgccggtc accaattcag acggcgcgcc ggtgtccacc
    25741 gttttggatg aaagctacaa agtcgctatc acgcgctttg acaacagaat ccgcgtcggc
    25801 ggcatggcag aattgtcggg ctacgaaatc aaactgcccg aaaaacgccg cgaaaccttg
    25861 gctttggtcg tcaacgattt gttcccggaa ggcggcgatt tgagccaagc attgttctgg
    25921 agcggcctca gaccgatgac gcccgacagc acgccgttaa tcggccgcac ccgctttgaa
    25981 aacttattct tgaatacagg ccacggcact ttgggctgga ccatgtcgct gggttcggca
    26041 aaattaaccg ccgatatcgt gagcggcaaa gacaccgaaa tccgaagcga cgatttgagc
    26101 ctgtcgcgct atcaggctta agctggaaag cgataaggtt tagcgacatt tcaaatttaa
    26161 aggccgtctg aaaatcattt ttcagacggc cttttgtttc aaaattcggg tatccaatca
    26221 ataaaataaa taataactat tatcatacgc attaaaaagt gttagtctga acctgtccca
    26281 gcttttagcg caggttttcc catgcccgat ttttcgatgt ccaatttggc cgttgccttt
    26341 tccattacgt tggctgccgg tttgtttacc gtattaggca gcggcttggt gatgttttcc
    26401 aaaacgccca atccgcgcgt gttgtcgttt ggtttggcat ttgccggcgg tgcgatggtg
    26461 tatgtttccc tgacggagat tttcagtaag tccagcgagg cgttcgctga aatttatgat
    26521 aaagaccacg cgtttgcggc ggcgaccatg gcatttttgg cagggatggg cggcattgcg
    26581 ctgattgacc gtctggtgcc gaacccgcat gaaactttag acgcgcaaga cccgtcgttt
    26641 caagaaagca aacgccgcca tatcgcgcga gtcggcatga tggcggcgtt tgcgattact
    26701 gcgcacaatt tccccgaagg cttggcgacg ttttttgcca cattggaaaa tccagcagtc
    26761 gggatgcctt tggccttggc gattgccatc cataatattc cggagggcat ttccatcgcc
    26821 gcgccggttt attttgccac ccgcagccgt aagaaaacgg tgtgggcgtg tctgctatcc
    26881 ggcttggccg agccgttggg ggcggctttg ggctatttgg ttttgcagcc gtttttgtcg
    26941 cctgccgtgt ttggttcggt attcggcgtg atagccggtg tgatggtgtt tttggcgttg
    27001 gacgagctgc tgccggctgc caaacgctat tcagacggcc atgaaaccgt ttacggcctg
    27061 acaatgggca tggcggtgat tgccgtcagc ctggtattgt tccattttta aaacctgtat
    27121 gccaaaggcc gtctgaaatg attcagacgg cctttttgcg ttataatccg cacatcaaaa
    27181 ataaccttaa tttcacaaag ttccatcatg ttagacaaat actcccccgc cgaaatcgaa
    27241 tccaaacact atcaaaactg ggaaagccaa ggctatttcc agcccgatat ggatttgacc
    27301 aaaccgtctt tttccatcca actgccgccg cccaatgtaa ccggcacgct gcacatgggc
    27361 catgccttca accaaaccat tatggacggc ctgacccgct actaccgcat gaaaggctgc
    27421 aataccgcct ggattcccgg caccgaccac gcgggcatcg ccacgcaaat cgtggtcgag
    27481 cgtcagcttg ctgcgcaaaa cgtgtcccgt catgacttgg gtcgtgaaaa attcttggaa
    27541 aaagtgtggg aatggaaaga agtttccggc ggcacgatta cgcagcagat gcgccgtgtg
    27601 ggctgttctg ccgactggac gcgcgagtat ttcacaatgg acgatgtacg cgccgaaacc
    27661 gtaaccgaag tgttcgtacg cctgtatgaa cagggcttga tttatcgcgg caaacgcttg
    27721 gtgaactggg atccggtgct tggcacggcg gtgtctgatt tggaagtaga aagcgtggaa
    27781 gaacaaggct ctatgtggca catccgctat ccgctggcgg acaatcctgc cgaagccgtt
    27841 atcgtggcga ccacccgtcc cgaaacgttg ctgggcgacg ttgccgtagc cgtcaatccc
    27901 gaagacgaac gttacaccca cttaatcggc aaagaattaa tcctgccgct gaccggccgt
    27961 accatccccg tgattgccga caaatacgtt gaaaaagact tcggcacagg ctgcgtgaaa
    28021 atcacgcctg cacacgactt caacgactac gaagtcggca aacgccacga cacgcgcctg
    28081 attaatgtgt tcgacttgga agccaaagtg ctggcaaacg ccgaagtgtt caacttcaaa
    28141 ggcgaagcgc aacaaggctt tgccctgcct gaaaaatacg caggtttaga ccgctttgcc
    28201 gcgcgcaaac aaatggttgc cgatttgcag gaacaaggct tgttggtcga aatcaaagca
    28261 cacacgctga tgacacccaa aggcgaccga accggctcag tgattgagcc aatgctgacc
    28321 agccaatggt ttgtcgccat gtccgccacg ccaaacggcg gcgagcctga cagcgaattc
    28381 aaaggcttaa gccttgctga caaagccaaa aaagccgtcg acagcggcgc ggtacgcttt
    28441 atccctgaaa actgggtcaa tacctacaac caatggatga acaacatcca agactggtgt
    28501 atttcgcgcc aactgtggtg ggggcatcaa atccccgcgt ggtacgacga aacaggcaat
    28561 gtgtacgtcg cccgcaatca ggctgaagcc gaaaaacaag ccggcaaaac aggcttgacc
    28621 cgcgaagaag acgtattgga cacttggttc tcctccgcgc tcgtgccgtt ttccacgctc
    28681 ggctggccgt ctgaaaccga cgaactcaaa gccttcctgc cgtccaacgt cttggtaacc
    28741 ggctacgaaa tcatcttctt ctgggtggcg cgcatgatta tgatgaccac ccactttacc
    28801 ggcaaagtac cgtttaaaga cgtgtacatc cacggcatcg tgcgcgacca cgaaggcaaa
    28861 aaaatgtcca aatccgaagg caacgtcatc gatcctgtgg atttgattga cggcatcggc
    28921 ttggacaagc tgctggtaaa acgtaccacc ggcctgcgca aacctgaaac cgcgccgaaa
    28981 gtggaagaag ccaccaaaaa actctttccc gaaggcattc cgagtatggg cgcggacgca
    29041 ttgcgtttca ccatggcgag ctacgccagc ttggggcgca gcgtgaactt cgacttcaaa
    29101 cgcgccgaag gctaccgcaa cttctgcaat aaaatctgga acgccaccaa cttcgtcttg
    29161 atgaataccg aaaaccaaga ctgcggttac ggcgcaaccg ctgccgaacc gcgcggctac
    29221 tccttccccg atatgtggat tataggccgt ctgaatcaga ccatcgaaca ggttacgcaa
    29281 gcctacgaaa cctatcgctt tgatttgtca gcggaaaccc tgtacagctt cgtatggaac
    29341 gactattgcg actggtattt ggaactcgcc aaagtgcagc ttcaaaccgg ctgcgccagc
    29401 cgccagcgcg ccacacgcca taccttattg cgcgtactcg aagccgccct acgcctgctg
    29461 cacccgatta ttccgttcat taccgaagaa ctgtggcaaa ccgtcgctcc tatgtgcgac
    29521 gcgaaaaccg ccgacagcat catgctcgcc cgcttccccg aagccgacgg cggtgagatt
    29581 gttcagacgg cattcgagca aatgaccgtg ttgcaggatt tgattggcgc agtccgcaac
    29641 ctgcgcggcg aaatgggcat ccagcctaat gtcaaagctc cgctgtttat cgaaagcgcg
    29701 gacgacttgg cggactacct caaatacctg ccgatgatga cccgcctgac cgaagcgcag
    29761 caagtcgccg ccctacccga aagcgaagac gcgcccgtcg ccgtctgcaa cggcgcgcgc
    29821 ctgatgctga aagtcgagat cgacaaggcc gccgagaccg cccgcttggg caaagaagcc
    29881 gagaagctgc aaaaagcctt ggacaaactc aatgccaaac tctccaaacc cggctacacc
    29941 gaaaaagccc cggcgcattt ggtggaaaaa gacaaagccg atttggcgga attggaagac
    30001 aaaatggcaa aagtgcaaac tcagttgtcg aagttgaaag actgatttga ttgaatcaac
    30061 aaggccgtct gaaaaatgtt tttcagacgg cctcttttat agtggattaa caaaaatcag
    30121 gacaaggcga cgaagccgca gacagtacaa atagtacggc aaggcgaggc aacgccgtac
    30181 tggtttttgt taatccacta tacatggcat tgcatcggca ctgatgaaaa cattcacagt
    30241 gatgcaaaag tcgaatatct tcaggacggc agtttccgtg gcgatgctat actcaaaatc
    30301 gatgatgacg gcaatatctt ggcataccgt gttgtcggct cagggaaatg gcgttttgaa
    30361 aacaatgcct gcgcctagtc gcggataaaa cttaccccgt gaagctgcga ctgcataatc
    30421 gccgccttca tcgccgacaa cgccttcgga cggacaaaat tgcggcggta cgccaatacg
    30481 acccgccggc ttggcggcgt accttcaaac ggaataatgc tgaacagcat atgatcgttt
    30541 tcggtcagcg cggttgccgg taacacgctg attgccaaac cgctggcgac catatggcgg
    30601 attgtattga tcgagctgcc ctgcaatgta ttggtcaacc cctgtatacg ttgtttcgcc
    30661 gccaattcgg aacagcttga gagtacctga tcccgcatac agttgccttc cgtcagcagc
    30721 aaaacctgct cctcacccag catccgtggc gaaacggcat ccagttcctc aaatgaatgc
    30781 cctttcggga caatcacgaa aaacggttcg tcatacaagg gttcggtaac aatgcccggc
    30841 tcttgaaacg gttcggcaac gataatcgcg tcaacgtccc cgcgtttgag cgactcggtc
    30901 aaagtatgcg tgtaattctc ttccaacatc aaaggcattt tcggtgcagt acggcgcaac
    30961 gagacaatca gtttcggcag caggtacggc gcaaccgtaa aaatcagccc gagtttgaac
    31021 gcaccctcca gctcgttttg ttcttcattt gccaaatgcc tgataagctc tgcctcttcc
    31081 aacaccttac gcgcctgtgc aacgatacgt tcccccgcct cggtcgtaat aatatcgttg
    31141 ctgctccggt caaacaaaga gacggcaagc tcttcttcca atttcttaat ggcaatagac
    31201 aaagtgggct ggctgacaaa acaacgccgc gccgccctgc cgaaatgacg ttcttgggcg
    31261 actgcgacga tgtaccgcaa ttcggttaag gtcatgtcct ataccttttt ctgttccggc
    31321 aaagtaatgt tcaactcaag cacatccata ccatcctgct tttcttggga aatacggata
    31381 ttgtctaatg aaacattcac atatttggac aggacttcca tcaactcttt acgtaaagtc
    31441 ggcaggtaat ccggagcctg accttcttgg gcgcgctctt gggcaatgat gatttgaagg
    31501 cggtcgcggg caacggttgc cgttttctgc tttctgccga ataaaaattc gattaatgac
    31561 ataccttatc ctccaaacag acgtttgaag aagctttttt tctcagcttc caagaaacgc
    31621 atttcacggt tctcgcccaa aagacgggca ataacgtcct tatatgcctc ggaagccgcc
    31681 acgctgtcct gatggatgac cggttctccg gaattggatg cctgcaagac gttttgggat
    31741 tcaggaatca cacccagcaa aggaatatgc agaatatcgc aaatatcctg tacagacagc
    31801 atttcgcctt ttgccacacg ttcgggagaa taacgcgtaa tcaacagatg ttctttaacc
    31861 gaaccgcctt gctctgcctt atgggatttg ctttgcaaaa ttcccaaaat cctgtcggag
    31921 tcacgcacac tggaaacctc aggattggtc gttacaatgg cttcatcagc aaaatacaac
    31981 gccatcaatg caccctgctc aataccggca ggagagtcgc aaataatata ctcaaagccc
    32041 attttcttgc cggacagctc ctgcatcact ttttctacgc cctcgcgtgt caaagcgtct
    32101 ttatcccgag tctgggaagc cggcaaaata aacaggtttt cacaattttt atctttaatc
    32161 aaagcctggt tgagcgtcgc ctccccctga atgacattga tcaggtcata aacgacacga
    32221 cgctcgcaac ccataatgag gtcgaggtta cgcaaaccca catcaaaatc aattaccgca
    32281 gttttatatc cgcgtaatgc caaacctgtc gcaatactgg cactggtagt cgttttaccg
    32341 acaccgccct tacctgaagt tactacaata atttttgcca caatatttcc tttttaaaat
    32401 atcaaacaat tactctgagc caattgcact gataaccaac cgattatcct gcaacaatat
    32461 ctgtaccggc tgcttgtgca gatgatccgg caaatcctgt tcaaaattac ggtaaatacc
    32521 cgccacagaa accagttctg cctgcatgga gtggataaat atgcgggcag aagtatcacc
    32581 cttggcgccg gccaaagcac gccccctcat cggcgcataa atatgtatat tgccatccgc
    32641 aatcaattcc gccccctgac tgaccgcccc cgtaacaatc aaatcgccat cttcggcata
    32701 aacctgctga ccggtacgga caggggatgt aatcaatact gttttccttg ctttctgacc
    32761 atcctccgtg ttctgcacct caacctgacc cagttcttta acattttccg aatgagacag
    32821 acaaaacagc aaatgatact tcatagccgc agcagcccaa cgttcattag aatgcttcag
    32881 acccaaaatt tgcataccat gccttgaaaa caacgaaacc aatgcagcaa gatccaaaga
    32941 ctcgggataa tcaaactctt gaacatccag cacaaatgga acaacaccag actcttgaaa
    33001 cttcttgccc aatttgacca gcacatcttc caaatcaaac aagtctgacg tatgcaaaga
    33061 tatagacaat acgtccatct ttgtcgactt tatatcgaaa gcattcatta tataaaccat
    33121 catagaattt tttaatgatg taagtttacc gcgcaaacct tagctattca attaatattt
    33181 taaacatgat agccaatacc ctattaccta tcagatattg caaaaataac gccacctgct
    33241 tttcggcaga tggcgttata tgactaaaat tactcagcag ccggtttgtc aaccaattca
    33301 accagtgcca gaggtgcatt atcaccttta cggaatccgt atttcaacac ccgaacataa
    33361 ccaccgttac gagcagtaaa acgaggaccc aaatcgccaa acagttttac tacaacatca
    33421 cggtcgcgag tacggtcaaa tgccaaacgg cggtttgcca atgacggctt tttacccaat
    33481 gtaatcaacg gctctactac acggcgcaat tccttggcct taggcagagt tgttacaata
    33541 gtttcgtgag tcaataatga attcgccata ttacgcagca ttgcagcacg atgactgctg
    33601 gtacggttta atttgcgatt gccattacga tgacgcatat caattatcct ttaattcttc
    33661 attaaggctt ttccaagcct acaggtggcc atgcttccaa cttagaaccc agtgtcaaac
    33721 ctttagatgc caatacttcc ttaatctcat tcaaagattt acgtcccaaa ttcggcgttt
    33781 taagaagctc ggtttcagtg cgttgaatca aatcgccaat ataataaata tcctcagctt
    33841 tcaaacaatt agctgaacgt actgtcaatt ccagatcatc caccggacgc aaaagaacag
    33901 ggtcgatagg aggtgctttt tcttcaacct cctccacagg cgtaccctgc aaatcagcaa
    33961 aaatagacat ctgatcaatc aaaatacgtg ccgcactgcg tacagcttcc tcaggatcaa
    34021 tagaaccgtc ggtttcgata tccaaaacca acttatcaag atccgtccgc tgctctacac
    34081 gtgcaggttc aacctcaaag ctaacacggc tgatgggcga aaagctcgca tccaactgga
    34141 ttgcaccaat ctgacggttc tcatcacgaa ctacctgacg acctgaaaca gattgataac
    34201 cacgaccttg ctctacttta atttccatct caatttgacc gttatcagcc aaatgacaaa
    34261 tgacatgacc aggattcaga atttctacat catgcggcaa ctcaatatca ccggcagata
    34321 cgacacctga acctgatttc ttcaacacaa gttgaacttg gctacgacca tggagtttaa
    34381 acacaatacc tttaatattc agcaaaatgt caacaacatc ttcctgaata ccatcaacag
    34441 tagaatattc gtgtaataca ccggcaatag ctacttcagt aggagcaaaa ccattcatgg
    34501 atgacagtaa gatacggcgc aaagcattac ctaaggtatg accgaaacca cgttcaaatg
    34561 gctgcataga tacttttgca cgagttgcag aaaaagtatt tacatcaatt tgacgaggtt
    34621 tcaaaaattc ggttgtgcta ttctgcattt aactgtccct cactgagcta gcattattta
    34681 gagtagaact ctaccaccag ctgttcatta atatcaccgg tcaattccga gcgatccggc
    34741 atgtttttga acacaccctc aagtttatcc gcatctacag aaacccaacc cggcaagccg
    34801 atttgagttg ccaaacccaa tgcttcttga atacgtacct gttttttggc tttttcacga
    34861 actgagacaa catcaccagc tttcacttgg aaagaaggaa tattgacaac ttgtccattc
    34921 acaactatcg ccttatgaga aacaagctgt cttgcttcag ctcgggtaga accgaaaccc
    34981 atacgataaa cgacattatc caaacgagat tccagcaact gcaacagcaa ctcgccggta
    35041 gaacctttac gacgatcagc ttctgcgaaa taacgacgga actgacgttc taatacgcca
    35101 taaatacggc ggattttttg tttttcacgc aactgcaaac catagtctga caaacgcggt
    35161 ttttttgcac catgctgacc gggagcggaa tcaattttac atttagaatc caaagagcgg
    35221 cgcgcactct tcaaaaacaa atccgtacct tcgcgacgtg ccaacttaca tttagggcca
    35281 atataacgtg ccatgtttca aatcactcca atattaaata cgacgttttt taggcggacg
    35341 gcaaccgtta tgaggcaacg gggtaacgtc agtaatgctg gtaatcttga aaccaagagc
    35401 attcaaagca cgtacagagg attcacgacc tggacctgga cctttaatac gaacctctaa
    35461 atttttaacg ccatactctt gggcaacttt accagctgct tctgctgcaa cttgtgctgc
    35521 aaatggtgta cttttacgag aacctttaaa accagcgccg ccagaggtag cccaagacaa
    35581 cgcattgcct tgacggtcag tgattgtaat gatggtattg ttgaaagatg catgaacgtg
    35641 cacaataccc tcactcacgg ttttacgtac ttttttacgt acacgtgaag ctgtgtttgc
    35701 tttagccatt aataaaattc cttaaaattt atttcttacc agcaatcgct ttacgcggac
    35761 ctttgcgggt acgcgcattt gtacgagtgc gttgaccgcg gcatggtaag ccgcgacgat
    35821 gacggaagcc acgatagcag cccatgtcca tcaatcgctt gatactcata gttacctcac
    35881 gacgcaaatc accttctact tcatacttgg caacttggtc acgcaaagca tctaattgag
    35941 tctcgtccaa atcttttgct ttagtatcag gcgcaatatt tgcagcctca caaatcaatt
    36001 tagcacgagt agcaccaata ccgtaaatag cctgaagacc aattacgatg tgtgcgttat
    36061 tagggatatt tacccctgca atacgagcca tattttttcc tttttagggc aaagtgtgtc
    36121 actatatcac agaatcacat taaaagaaat attccattaa ccttgacgct gtttgtgacg
    36181 gagatcagta caaattacac gaactacacg atttcgacga ataatcttgc aatttcggca
    36241 aattttctta acagatggtt gtacacgcat tttattttcc ttttattggt ttatcttgct
    36301 ctgaaaacga ttcgagccct agttagatca taaggtgtca gctctactgt gaccttatct
    36361 cccggagaaa tacgaatgta atgcatccgc atcttcccag aaatatgacc caatacaata
    36421 tggtcattct caagttttac tttaaatgtt gcattaggta aagtttcaag aatttcacct
    36481 tgcatttgga tagtatcttc tttcgccata attctatttc cgagaaaatg atttcatttc
    36541 tgaacgagtc attaacttat catactgttg agtaagccta tacgaattta tttgtgtact
    36601 aaaatccatc gttacaacaa ctagaatcaa caaagacgtg ccacccaaat aaaaaggtac
    36661 atttaaaacc gtagttaaga actctggaat taaacaaata gttgtaatat aaagagctcc
    36721 aaacaatgtc aaacgtaata caactttttc taaataccta gaggtctgct caccaggtct
    36781 aatcccagga acaaaagcac cactcttttt taaattctct gccatttctt tagggctaaa
    36841 aaccaaagcc gtataaaaat aacaaaagaa aataactgtc gctgcaaata aagccatata
    36901 cagcaattga ccgtgttgta acaatccagc tattttgtgc aaaacactat ttgtatcagc
    36961 cgaaccaaac caacctaaaa gagtagatgg aaatagaata atactggaag caaaaattgg
    37021 gggaataaca ccagccatat tcaacttgaa aggcatatgc gtattttgac tacccgccct
    37081 accattaaac tggcgttttg cataatgaat aggaatcttc cgctgtgcac tttcaaagta
    37141 tacaaccaaa taaattaata ataaggcacc aaatacaata aacaacgccg taagcatgct
    37201 catagaacct tggttcgtca gtgtaaccag ctttgcaata cccgaaggaa tacctgaagc
    37261 aatacctgcc gtaatgatta aagaaatacc gttcccgata cccctttcag taatttgctc
    37321 cccaagccac ataagaaaca tggttcccgt taccaaagaa actaccgtgg aaacatgaaa
    37381 ctcaaatgaa cttgttacaa caattccttg ctgaaatacg aaagatgcaa cacctagact
    37441 ttgaagaatt gctaacaaaa cagtaccata cctagtatat ttcgtaatta cctttctacc
    37501 agcctcccct tcttttttta aagccttcaa tgatggcaaa atttcagaag caagctgtac
    37561 aataatagaa gctgaaatat atggcataat tcctattgca aatatactaa agcgctctaa
    37621 cgacccaccg gaaaacatat tcaatattcc caggatgccg tttccagcgc tttcgtataa
    37681 tttagctaaa gcaacagcat caactccagg tacgggtata tgggcaccaa ttcgaaaaac
    37741 aatcaatgct ccaaatagaa acaaaagacg tttcttaaga tctccaaatt tggatgaacc
    37801 tgatgacgtt tgttgattag ccacaatatt aaaccttaca tttcaatctt accaccaaca
    37861 gcctcgatag cagctctcgc acctttggta actttaatac ccttcaaagc aactgcctta
    37921 gaaatttcac cagaagcaat aactttaaca ttagagactg tagatgcaat cagacccgct
    37981 tgctttaaga ccaaaatatc aatctcatta acagcaattg attccagttc acttaaacga
    38041 agctgtgcat tagctgatgc tgttaaagat ttaaaacctc ttttagggag gcgtcgttgc
    38101 aagggcattt gaccaccctc gaaacccacc ttatgaaacc caccagaccg gctcttttga
    38161 cctttatgac cacgaccacc cgttttgcca agaccactac caataccgcg tccaacacga
    38221 cgaccagcat gcgtagcacc aacagcaggt tgaattgtat tcaaaaacat atcaagactc
    38281 cactttcaac aagtagctga ttttattaat cataccacgg ttttcagggg tatctaaaac
    38341 ctctaccgta tgctcgcgac gacgcaaacc taaaccgcgt gcacatgcac gatgagattc
    38401 aattgtacca atcaggcttt taaccaatgt aaccctaatc tttttttgtt cagccatggt
    38461 taactcccaa aatgtcttcc actgtcaagc cacgtttggc tgcgatatca gcaggagtat
    38521 gcaacttaga caaaccatct aatgttgcac gtacgatatt atatgggtta gtagatccgt
    38581 gcactttggc ggagatatta tgaatgccca tagcatcaaa aaccaaacgc ataggtccac
    38641 cggcttttac gccactaccc tctttagcag gctgcataaa tactttagta gcaccatgtc
    38701 gaccaataac ctcatgatga atcgtaccat tttttaatgg taccttaatc atagagcgtc
    38761 gtgcttgatc catcgccttt tggactgcaa caggcacttc ttttgactta cctttaccca
    38821 taccaatacg accatctcca tcaccaacaa cagttagcgc agagaaagcc ataatgcgac
    38881 cacctttaac tactttggtt acacggttaa ctgcgaccat cttttcaatc agaccgtcac
    38941 cgcgttcttc aatttcatgt tttgccatct gaaagtctcc aaatatttag aagcttaaac
    39001 cattttcacg agcagcttca gccaaagcct tcacacgacc gtgatattgg aaacctgaac
    39061 gatcaaaagc aaccttttct acacctgctg ctttagcctt ctcagcaata cgtttaccaa
    39121 ctattgcagc tgcttcaaca ttgcttccag atttcagact accgcgcacc tcagcttcca
    39181 atgtagaggc ttgagccaat actttatcac cttcagcact aattacttga gcataaatat
    39241 gattattgct tcggaacaca cataatctta ccattttcaa gtccgcaata cgagcacggg
    39301 ttttgcgtgc acgacggagt cgggttgtat gtttatccat tagtgaacct caattatttt
    39361 ttcttggctt ctttcattac cactacttct cctacatagc gaacaccttt acctttataa
    39421 ggctcaggag cacggaacgc acgaatctca gcagcaactt gaccaacaac ttgtttatcc
    39481 gagccggtta aaacaatctc tgtttggcta ggagtttgaa cggagacacc ttcaggcatt
    39541 tcatatacga tcggatgaga aaaacccaaa gacagattca agattttacc ttgtgcttga
    39601 gcacgataac ccacacctat caattgcaat cttttctcaa aaccttctga aacaccttta
    39661 accatattgc tgactaatgc gcgagcagta ccagacattg catttgcttg tttactgctg
    39721 ttattcgcaa caaaagccaa tttgccatca ttaaattcaa tggctacatc agaatgcaaa
    39781 ggaaaagaca attcaccgtt cttaccctta ataactaatg cctctgttcc aaattttact
    39841 tctacaccag cgggaacagt cactgggttt tttgcgacac gtgacatttc ttttcctcca
    39901 ctaggctaca atgcataaca actcaccacc aacaccttga gaacgtgctt tacgatcagt
    39961 cattacacct ttagaagtac taacaatagc aatacccaag ccattcataa cacttggaat
    40021 ctcactagac gctttataaa tacgcaaacc ggggcgagat acacgcttga tttgttcaat
    40081 tacaggacga cctgcatagt attttaattg aatttccaat atagacttta cgtcagatga
    40141 aactgcgaag tcctcaatat atccttcttc tttcaatacc tttgcaatag cacactttaa
    40201 ttttgaagaa ggcattgcaa ccgctgcttt attagcacgt tgcgcattgc ggatacgagt
    40261 caacatatcg gaaataggat catgcatact cattcttaat tactcctatt accagctggc
    40321 tttaacaaca cccggaattt cgccacgcat ggcgatttca cggattttaa tacgacccaa
    40381 accaaattta cggaaagtac cacgagggcg acctgtcaaa gcacaacgac gacgttgacg
    40441 cacaggtgcc gcattacgag gaatggattg aaacctcaaa cgagcctcaa aacgctcttc
    40501 ctcagttgca ttcgaatcat tgattaccgc tttaattgcc gcgcgtttag ccgcatattt
    40561 tttagccaaa gcttgacgtt tcagattacg attaataagt gctttcttag ccatgattat
    40621 cctttgaacg gaaatttaaa caatgacaat aaagctttcg cttcctcatc ggtttttgct
    40681 gtagtagtaa tagtaatatt caaaccacgc aaagcatcaa ttttatcgta ttcaatttcc
    40741 ggaaaaataa tttgctcacg aacacccata ttgtaattgc cacggccatc aaatgattta
    40801 ccgctcacac cacggaagtc acgtacgcga ggtaatgcaa tagtaatcaa acgatccaag
    40861 aattcaaaca tttgatcacg acgcaatgtt actttgcaac caaccggata gttatcacgg
    40921 attttaaaac ctgcgataga tttacgggca acagtaacaa ccggtttttg accggcaatt
    40981 ttctctaaat cggaaacagc atgttccata acttttttgt cagcaacagc ctcacccaca
    41041 cccatattca aggtgatttt ttcaatacgc gggacttcca ttactgattt gtaaccaaat
    41101 tgtttaacca attcaggaac aactgtctct ttataaaact ctctcaaccg agccatgtta
    41161 tctccttatg ccccaatgat agagccattt gatttgaaga aacgaacgcg tttaactttg
    41221 ccttcatttt caatcagctt aataccaaca cggtccgctt tattagtttc cggattcagg
    41281 attgcgatat tagaaatatc caaaggcatt tctttagtaa taataccgcc ctcaatgcca
    41341 cgcattggat taggtttttg atggcgtttt acaacattaa cgccctcaac aacaacttta
    41401 tcacccaaca ctcgaactac ttgaccctgc ttacctttat ccttaccagc aattactaca
    41461 accctatcgc ctttaatgat tttattcatc gtgccattcc ttataatact tcaggtgcca
    41521 atgaaacgat tttcataaat cgctcagtac gcaattcacg ggttaccgga ccaaagatac
    41581 gagtacccaa aggttcaagt ttattattca gtaacacggc ggcattgtta tcgaatttaa
    41641 ttaacgcacc atcaggacga cgtacaccct tagcagtacg aacaactacc gcattatata
    41701 catcgccttt tttgacacgg ccacgcggag ccgcatcttt aactgccact ttaataatat
    41761 cgccaacaga agcgtagcga cgcttagatc cgcccaatac cttgatacac attacgcgac
    41821 gcgcaccaga gttatcagcc acatctaaga tggtctgcat ttgaatcatg ttattacctt
    41881 taaattaacc aacttaattt accactttca gataacttac agcaaagccg ctgcttatta
    41941 aatgaaacct tgcggttcaa gtaaaccagt cttggacccc gaaagggaag aaacttccag
    42001 aagaaactga aagacaggaa acgaagttta cacgcaaatt aaatttgctg caatacttcg
    42061 tttcccattc caagcacgtt gctttaattc ttaaatagaa cgtgctttct caaccagctc
    42121 actgacaacc caagatttag tttttgacaa tggacgggat tccgatataa caaccacatc
    42181 accaattcca tattgattat tttcatcatg ggcatggatt ttagtagata accgaataat
    42241 cttaccatac agcggatgtt ttactttacg ctcaaccaat actgttacgg ttttatccat
    42301 tttgtcgctt actactttgc cttgcaaagt acgaacattt ttagtttcgc tcattactta
    42361 gcaccttttt cagttaaaac ggttttaata cgagcaatat cgcgacgtac acgtttcaat
    42421 tcacttggtt tgcctaattg accggtagcg ttttgcatac gtaagccaaa ctgagctttc
    42481 aacaagtcca acaaatctgc attcaactgc tcaacggatt tgtctttcaa ttcatttgct
    42541 ttcattattg acccacctgt cttactacaa aggttgtagg aataggcaat ttggcagcag
    42601 ccaactcgaa tgcttcacga gccagttcct ctggaacgcc atccatttca tacaacactt
    42661 tacctggttt aatttcggca atgtaatatt ccacgttacc tttaccgcca cccatacgaa
    42721 cttgaatagg cttttcagta atcggtttat cagggaatac acgaatccaa atacgaccac
    42781 cacgtttgat atgacgggtc attgcacgac gagcagcttc gatttgacgg gcagtcaaac
    42841 gaccacgacc tacggctttc aagccgaact caccgaaact taccttatta ccgcgagtag
    42901 cgatgccagt attgcgaccc ttttgttgct tacggtattt cagtctagtt ggctgcagca
    42961 ttacgtctac ctgcctttct ttgtttactc tcatgttcag gtttggaaga tttaatattg
    43021 ccttccgtat aaacccaaac tttcagaccc aatacaccat atgtggtgtg cgcttcgctg
    43081 gttgcataat ctacatttgc acgtaaagta tgcagtggca cacgaccttc acgataccat
    43141 tcgctacggg caatatccgc accattcaga cggcctgaag tcataatctt aatgccttta
    43201 gcaccagaac gcattgcatt ttgcattgct cgtttcatag cacgacggaa ttgaacgcgc
    43261 ttttccaact gctgggcaat accgtcagca ataatttgag catccaactc aggacggcga
    43321 atctcttcaa tatttacatg aacaggtaca cccatcaaga cttgcaagtc acgtttcaaa
    43381 acctcgatat cctcaccttt tttaccgata accacacccg gacgagcgga gtgaatggta
    43441 atgcgtgcag attttgcagg gcgttcaata accactcgac caaccgaagc attggccaat
    43501 ttttgacgca aataattgcg aacatcgata tcctgcttca aaacagtaga aaagtcggtg
    43561 cttttagcaa accattttga agcccagtct ttagttaccg ccaggcgaaa gcctgtaggg
    43621 ttaatctttt gtcccatagc ttttccttag ttacccactg tcacattgat atgacaagtt
    43681 tgtttttcga tgcggttacc gcgacctttg gcgcgagctt gaaaacgttt caagcttggg
    43741 cctttgtcaa caaagatagt taccactttc agttcatcaa tgtccgcacc gttattgtgc
    43801 tcggcattag caatagctga ctccaatact tttttaatca gctcggcacc ttttttagga
    43861 ctgaaagcca aaatattcaa agcttgggca acgtctttac cacgaatcaa atcagctacc
    43921 aaacgagcct tttgagcaga gatacgggca tttttatgtt gtgcatttac tctcatgatt
    43981 caccttattt ctttttagcc tttttatcgg ccaagtggcc tttaaaggta cgggtcaatg
    44041 agaattcgcc taatttatga ccaaccatat tgtcgctgat aaacacaggc acatgggtgc
    44101 ggccgttgtg cacagcaatg gtcagaccga taaaatcagg cagaatggta gaacgacgag
    44161 accaggtttt aatcgggcgt ttgtcgttgc ttgcgcgagc agcatctact tttttcagca
    44221 aatgcaggtc tacatatggg ccttttttca atgaacgagc catactaaat taacctttat
    44281 ttgagtaacg gcgacgaaca atcatgttat ccgtgcgttt gttattacga gtgcggtagc
    44341 ctttagcagg agtaccccat gggctgaccg gttcgcgggc ctcgcccgta cggccttcac
    44401 caccaccatg cgggtgatcg acaggattca tgacaacacc gcgtacggtc ggacgaatac
    44461 cgcgccaacg gttggcaccg gctttaccga tttttttcag gctttgctct tcgttaccaa
    44521 cttcaccgat ggtcgcacgg caatttacgt tgattttacg aacttcgcca gagcgcagac
    44581 ggacttgagc gtacgcgcct tctttagcca gcaataccgc agaagcaccg gcagaacgtg
    44641 cgatttgagc acctttaccg ggtttcattt cgatacagtg gatagtcgta ccaacgggaa
    44701 tgttgcggat cggcagggtg ttgcctactt tgatggcagc ttcagcaccg gaaaccaata
    44761 cggcaccgac ttgaataccg cgaggagcga tgatgtaacg acgctcgccg tctgcatagc
    44821 acaacagtgc gatgaaggca gtacggttag gatcgtattc gatacgctct accttagcag
    44881 gaatgccgtc tttgttacgt ttaaagtcta caacacggta atggtgttta tgaccgccgc
    44941 ctttgtgacg ggtggtgata tgaccattac tgttacgacc ggcagtagaa tttttctttt
    45001 cgagcaaagg tgcataaggc gcacctttgt gcaaaccttc tgttaccacg cgaaccatgc
    45061 cgcgacggcc tgcagaggtc ggcttcattt taacgattgc cattttgttt attccttatc
    45121 tgcagctgca gcagcggctt ccaaatccaa ctcttgaccg gcagccaagc ttacataagc
    45181 ttttttaaca tcgctgcggc ggcccaaagt acgaccaaaa cgcttagttt tgcctttagt
    45241 tgtaacggta gttacagaag caacttgaac accaaacagc agctcaacag ccgctttgat
    45301 ttcaggtttg gttgcatttg ccaaaacttt aaacgtcatt tggttgcgtt tttcagccaa
    45361 tacgttgctt ttttcagaaa cgataggtgc caaaatcact tgagtcaaac gttgttgatt
    45421 catacccatt gctcctctaa ttgtgcaact gcatctttag tgatgattac ttttttgtaa
    45481 cgcagcaagc tgtaaggatc aacttgttga gcttccaaaa ccaacacgtt tggcaagttg
    45541 cgtgaagcca agtaaacatt ctcgtcgagc tgtttggtta caaacaacac ttgctctaga
    45601 cccagatttt tcacttgttc agcaaaaact ttggttttag gagtttcggc agtcaacgcc
    45661 tcaatcgcaa acaaacgctc gtcacgagtc aattgggaca gaatagtcgc cataccggca
    45721 cggtacattt tgcggtttac tttttgagtg aagttttcgt cgggtttgtt cgggaacgcg
    45781 cgaccacctt tacgccacag cggagatgaa gtcataccgg aacgggcgcg gccggtacct
    45841 ttttgacgcc atggtttttt ggttgagtgt tttacttcgg cacgggtttt ttgagcacgg
    45901 ttaccagagc gggcgtttgc caagtaggca tttaccagct gatgaaccaa cgcttcattg
    45961 tattcgcggg cgaacaaagc atcagaaaca gacaggctgc ctgaaacttg tcctttagcg
    46021 tcaattactt tcaattccat tacgcaccta ctttcacgct gggacgaact acaacatcgc
    46081 tgttgaccgc acccggaaca gcacccttaa ccaacagcag ttggcgttct gcatcaacac
    46141 ggacaacttc caatttttga acagttgctt tggtgttgcc gtattggccg gccatgcgtt
    46201 taccggggaa cacgcgaccc gggtcttgcg ccataccgat agaacctgga acgcggtgag
    46261 aacgggagtt accgtgggaa gtacgttggg caccgaagtt atgacgttta atcgtgccgg
    46321 agaaaccttt acctttagag ataccggtta catcgaccag ttgaccgact tcaaacatag
    46381 aaacggtgat ttcgtcacca gctttcaatt cagccagttt ttcttcagtc aaagcaaact
    46441 caatcaaacc gcgaccggct tcaacacctg cttttgcaaa gtgcccggct tcggctttgt
    46501 tgacacgatt ggcttttttc tgaccaaagg taacttgaac ggcagtatag ccgtcagtat
    46561 ctttggattt tacttgtgta acgcggttgg cagacatatc caaaacggtt accggaacag
    46621 aaacaccctg ttcgtcgaac acgcgggtca taccaacttt gcgtccaacc agacctaaag
    46681 tcatgattat tttcctttta aagtaaaggg acaggctacg attggcctgt ctttcagaca
    46741 aagttaaact tggcacaaat gtaccaagct cggcattata tctgacaagt taaagaaata
    46801 tcaacagaaa tatcagaaat atcttcaata tgccgtctga agcgttttga attttcagac
    46861 ggcatagggt cggactcttt taaagggtcg gtatccggtc atcacagcgt atgcatcttt
    46921 ctccgacaaa ccggaatatg gttgatgact gtaatcgggg atgctcagtt cgtcaaagcg
    46981 gcaaaacagg ttgaaatcga tgcgggtgat gaggctgtg
//
LOCUS       DAWFJR010000003        45009 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWFJR010000003 DAWFJR010000000
VERSION     DAWFJR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 45009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45009
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.003"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="WGR04_000137"
     CDS             complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="WGR04_000137"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0793778.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAATTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGYKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1906..2343)
                     /locus_tag="WGR04_000138"
     CDS             complement(1906..2343)
                     /locus_tag="WGR04_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793779.1"
                     /translation="MGNFLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKA
                     THGPSVKNAVYAHQIETGLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQ
                     YSIFEYEVEHPENPNEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(2437..4044)
                     /gene="aceF"
                     /locus_tag="WGR04_000139"
     CDS             complement(2437..4044)
                     /gene="aceF"
                     /locus_tag="WGR04_000139"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ0793780.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAASAAAEAQPAPAAAPVA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGSVIIEVETVGSAAATPAQAAQAAAPAAAPPPTAAAAPAAAPAPS
                     APAAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQ
                     GGAAKPAAAGASLGSGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPH
                     VTVHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDN
                     LVLKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQG
                     ACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDH
                     RVIDGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(4194..6857)
                     /gene="aceE"
                     /locus_tag="WGR04_000140"
     CDS             complement(4194..6857)
                     /gene="aceE"
                     /locus_tag="WGR04_000140"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ0793781.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALSA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7552..9264
                     /locus_tag="WGR04_000141"
     CDS             7552..9264
                     /locus_tag="WGR04_000141"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ0793782.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLEFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERTAAQAELVKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFAGKVYADFATEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRDVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVASVTA
                     SLNG"
     gene            9327..9983
                     /locus_tag="WGR04_000142"
     CDS             9327..9983
                     /locus_tag="WGR04_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ0793783.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGMGEGEDILGCVAGYGVGLDLTA
                     RDIQCHLKAQGLPWLKAKGFRHSACVSDFVAAGRISNPEKVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9993..10448)
                     /gene="ruvX"
                     /locus_tag="WGR04_000143"
     CDS             complement(9993..10448)
                     /gene="ruvX"
                     /locus_tag="WGR04_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ0793784.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(10441..10989)
                     /locus_tag="WGR04_000144"
     CDS             complement(10441..10989)
                     /locus_tag="WGR04_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ0793785.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10991..11551)
                     /locus_tag="WGR04_000145"
     CDS             complement(10991..11551)
                     /locus_tag="WGR04_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ0793786.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNMLGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11879..13684
                     /locus_tag="WGR04_000146"
     CDS             11879..13684
                     /locus_tag="WGR04_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0793787.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLE
                     QKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRA
                     EMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGV
                     FYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAA
                     GSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13804..15288
                     /locus_tag="WGR04_000147"
     CDS             13804..15288
                     /locus_tag="WGR04_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ0793788.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAIPEDYVYGMGGDSLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
     gene            15617..17644
                     /gene="uvrB"
                     /locus_tag="WGR04_000148"
     CDS             15617..17644
                     /gene="uvrB"
                     /locus_tag="WGR04_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ0793789.1"
                     /translation="MEVIRYPDSPFKLHQPFPPAGDQPTAIADLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDGGKGRRQGKNKVKV
                     SEIHNEEDAVKEIAKLEKAMQQAARDLQFEEAAVLRDRIRGIKEGLLFGSE"
     gene            17756..18043
                     /locus_tag="WGR04_000149"
     CDS             17756..18043
                     /locus_tag="WGR04_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793790.1"
                     /translation="MTYSFRVENINDISDIPENHLEIVRAALPSNRSNALSREPDGAQ
                     AVFVYEIDGEFRAKLFDRIKDTDSYLQATEDNYSNDPRSIGVVYVLEQQQG"
     gene            complement(18224..18940)
                     /gene="trmB"
                     /locus_tag="WGR04_000150"
     CDS             complement(18224..18940)
                     /gene="trmB"
                     /locus_tag="WGR04_000150"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ0793791.1"
                     /translation="MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIDENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYARQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRMG"
     gene            complement(19062..20465)
                     /locus_tag="WGR04_000151"
     CDS             complement(19062..20465)
                     /locus_tag="WGR04_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ0793792.1"
                     /translation="MNNPDLPYRQALERLSQKQYNFTEVRRLLTEAALAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLIPLYRPLA
                     EAGLSEAQVRLMYLLYASRHFEEALEWAKTSTKNNNPHGQYLLAQYCRYGTPPDFETA
                     HLLYREAAAQGLAAAQWQLGLQYRFGQGTPTDTTQAINHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDKAVYRFQQAAQENDPDAHAALADIYLQGKHPERNYKLALHHAEAAAAERHP
                     EGLRILGDICRYGLGIAPDTEKARHYYRQAAEAGSLTAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAEQLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVGPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
     gene            complement(20583..22469)
                     /gene="uvrC"
                     /locus_tag="WGR04_000152"
     CDS             complement(20583..22469)
                     /gene="uvrC"
                     /locus_tag="WGR04_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ0793793.1"
                     /translation="MPSEAVFQIIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNLI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFSVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSNQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPHSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEQVEGISKALAETIY
                     NHLH"
     gene            complement(22524..24701)
                     /locus_tag="WGR04_000153"
     CDS             complement(22524..24701)
                     /locus_tag="WGR04_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ0793794.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVKSAGVNFASEEAQ
                     VTFDDSKTSAADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLAINIPFLIG
                     MVGMMLKGLNWTRHDWMLSPLLQFALASVVQLWLAVPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSPHAAYGMAHVYFEVGIMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRDGEWRQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGIGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGSPQVAAVYCVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQTVVGAGITAEVEGVGLVKA
                     GKAEFAELALPKFLDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNI
                     DVYIMSGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALSVSRATLRNIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            24840..25790
                     /gene="trxB"
                     /locus_tag="WGR04_000154"
     CDS             24840..25790
                     /gene="trxB"
                     /locus_tag="WGR04_000154"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ0793795.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            25946..26314
                     /gene="rpsF"
                     /locus_tag="WGR04_000155"
     CDS             25946..26314
                     /gene="rpsF"
                     /locus_tag="WGR04_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ0793796.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            26315..26617
                     /gene="priB"
                     /locus_tag="WGR04_000156"
     CDS             26315..26617
                     /gene="priB"
                     /locus_tag="WGR04_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ0793797.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHKSWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            26624..26854
                     /locus_tag="WGR04_000157"
     CDS             26624..26854
                     /locus_tag="WGR04_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ0793798.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            26871..27323
                     /locus_tag="WGR04_000158"
     CDS             26871..27323
                     /locus_tag="WGR04_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ0793799.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            27337..27444
                     /locus_tag="WGR04_000159"
     CDS             27337..27444
                     /locus_tag="WGR04_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HEZ0793800.1"
                     /translation="MPSETLPIVGMWFQTVFVLFSVYSAKIGMVVFIVD"
     gene            28024..28179
                     /locus_tag="WGR04_000160"
     CDS             28024..28179
                     /locus_tag="WGR04_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793801.1"
                     /translation="MYTGHYGGNTVWEAAASRGIGTAGDAPMPSGVKAGQSAVWKKAM
                     ESVGRRS"
     gene            complement(28295..29101)
                     /locus_tag="WGR04_000161"
     CDS             complement(28295..29101)
                     /locus_tag="WGR04_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0793802.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFAMMPGMIFGVFTGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(29102..29848)
                     /gene="pssA"
                     /locus_tag="WGR04_000162"
     CDS             complement(29102..29848)
                     /gene="pssA"
                     /locus_tag="WGR04_000162"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ0793803.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            complement(29922..30326)
                     /locus_tag="WGR04_000163"
     CDS             complement(29922..30326)
                     /locus_tag="WGR04_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793804.1"
                     /translation="MANRFQQRKSAYRRLSGKPLFSDGIAANLCGGRKTLSYNLSRFK
                     ISIRSEMQKISFNLLKPANSPKIGKRRPGLNGAPHIPMPSPRYLPASPEMPGNNIHPA
                     KIRNNTPGGRQSQTAPQSIRHQKNKPPPRASS"
     gene            30432..30914
                     /locus_tag="WGR04_000164"
     CDS             30432..30914
                     /locus_tag="WGR04_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEZ0793805.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFSELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGAMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(30966..32177)
                     /locus_tag="WGR04_000165"
     CDS             complement(30966..32177)
                     /locus_tag="WGR04_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ0793806.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWTAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            32400..34838
                     /locus_tag="WGR04_000166"
     CDS             32400..34838
                     /locus_tag="WGR04_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ0793807.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAVTLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNSKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPSPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDEPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTD
                     LNGSRKILAHKDHL"
     gene            35046..36356
                     /locus_tag="WGR04_000167"
     CDS             35046..36356
                     /locus_tag="WGR04_000167"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ0793808.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADPSRLQAPTSLAVESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            36452..37066
                     /gene="clpP"
                     /locus_tag="WGR04_000168"
     CDS             36452..37066
                     /gene="clpP"
                     /locus_tag="WGR04_000168"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ0793809.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQVLENRASLQL"
     gene            complement(37119..38384)
                     /gene="ispG"
                     /locus_tag="WGR04_000169"
                     /gene_synonym="gcpE"
     CDS             complement(37119..38384)
                     /gene="ispG"
                     /locus_tag="WGR04_000169"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ0793810.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(38400..39161)
                     /gene="pilW"
                     /locus_tag="WGR04_000170"
     CDS             complement(38400..39161)
                     /gene="pilW"
                     /locus_tag="WGR04_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ0793811.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLIGQ"
     gene            complement(39164..40258)
                     /gene="rlmN"
                     /locus_tag="WGR04_000171"
     CDS             complement(39164..40258)
                     /gene="rlmN"
                     /locus_tag="WGR04_000171"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ0793812.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTSAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(40401..40826)
                     /gene="ndk"
                     /locus_tag="WGR04_000172"
     CDS             complement(40401..40826)
                     /gene="ndk"
                     /locus_tag="WGR04_000172"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ0793813.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(40885..42036)
                     /locus_tag="WGR04_000173"
     CDS             complement(40885..42036)
                     /locus_tag="WGR04_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ0793814.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
     gene            complement(42427..43254)
                     /gene="fghA"
                     /locus_tag="WGR04_000174"
     CDS             complement(42427..43254)
                     /gene="fghA"
                     /locus_tag="WGR04_000174"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ0793815.1"
                     /translation="MKLIEQHQIFGGSQQVWAHNAQTLQCEMKFAVYLPNNLENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDTSPRGEQVPNDDAYDLGQSAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            complement(43263..44372)
                     /locus_tag="WGR04_000175"
     CDS             complement(43263..44372)
                     /locus_tag="WGR04_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ0793816.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINEAFELMHEGKSIRAVIHY"
     gene            44520..44927
                     /locus_tag="WGR04_000176"
     CDS             44520..44927
                     /locus_tag="WGR04_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ0793817.1"
                     /translation="MAYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
BASE COUNT        10822 a        11134 c        12132 g        10921 t
ORIGIN      
        1 ccggaggaaa tattttaatc cgctgtaaaa tagaaaatgc cgtctgaaca tttcagacgg
       61 catttgctca aataaacctt atcggctgtt tattcagtcg gatttatttt ttcttttgcg
      121 gaggcaggtc ggtacaagta cccaatgcca cttccgccgc cataccgatg gattcgccca
      181 aggtcgggtg cgggtggatg gttttgccga tgtctgccgc gtcgcagccc atttcgatgg
      241 caaggcagac ttcgccgatc atatcgccgc cgttaggacc aacgataccg ccaccgatga
      301 tgcggccggt ttcggcatca aaaatcagct tggtgaaacc attgtcgcaa ccgttggcaa
      361 tcgcacggcc ggaagctgcc cacgggaagt tggctttggt gattttgcgg ccggatgctt
      421 tggcggacaa ctcggtttcg cccacccacg ccacttcggg ggaagtgtag gcaacgccag
      481 gaatcacgcg cgcgtcgaag taggctttgt agccggcgca gttttcagcg gcaacgtggc
      541 cttcgtgaac ggctttgtgc gccaacatcg gttgaccgac gatgtcgccg atggcgtaga
      601 tgtgcggcac gttggtacgc atttgtttgt ccacttcgat gaagccgcga tcggttacgg
      661 caacgcctgc tttttccgcg ctgatgagtt tgccgttggg cgcgcggccg gcggcaacca
      721 atacggcatc gtagcgttgc ggctctttcg gtgcatttgc accttcaaag gtaacgtaaa
      781 cgccgtcttc tttcggctcg actgcaacgg ttttggtgtt gaccataatg ttgtcaaaac
      841 ggtattcgtt ttgtttttgc catactttca ccaagtcgcg gtctgcgcct tgcatcaggc
      901 cgtccatcat ttcaaccaca tccaaacgcg aacccagcgt gctgtaaacc gtacccatct
      961 cgaggccgat aatgccgccg ccgataatca gcagtttgcc cggtacttct ttcagagcca
     1021 atgcgccgct ggaatcgatg atgcgcggat cttcaggaat gaaaggcagt ttggttacgc
     1081 ggctgcctgc tgcaatgata cagtttttga aggcaacgat ttttttctcg ccggtagtgg
     1141 ctgcctgttc gtacgcgtcg ccggcagtca gcgacacttc caagtggtgc ggatctaaga
     1201 attgcccgtc gccttggata acgtccactt tacggctttt cgccatacct gccaaaccgc
     1261 ccgtgaggcg ggaaactacg ccgtctttgt aggcgcgaag catatcgatg tcgagttccg
     1321 gctcggggta tttgataccg ttggcagcca agtggcgcac ttcgtcgata acggcggcat
     1381 tgtgcaacag ggctttggaa gggatacagc cgacgttcag gcaaacgccg cccaaagttt
     1441 tgtaacgctc gacgatggcg actttcaagc cttcatcggc agcggcaaat gcagcggagt
     1501 aaccgccagg gccgccgccc aatacgacca cgtcgtactc ggcatcggca gaaccgccga
     1561 attgcgcggc ttgcggagca ggagcggcag ctttaggggc ttcttgcgcc ggggcggcag
     1621 ccgcttcggc tttaggggcg gcagccgtgc cttcagcttc aacgacaaca atcaaaccgc
     1681 cttcggagat tttgtcgccg actttgactt tcacttcttt gaccacgccc gcaacttcgg
     1741 caggcacgtc catcgtcgct ttgtcggttt ccaaggtaat cagggtatcg tccacagcaa
     1801 tagtgtcgcc cacgttcact tcaactgcga taatatctac attttcgtgt ccgccaatgt
     1861 cgggcacttt caattcaact aaagccatta tattgacctt tcaacttagt taatttctat
     1921 caactcttta gcaataatca cttcttcagg aatacagcca caatcttcag ctctgattgt
     1981 tacttccttc tcatttgggt tttctggatg ttcaacctca tattcaaaaa tagaatattg
     2041 accaaacaaa tccctattta aaacatatat atagccattt tcgatgccgg aacttgttgc
     2101 aaatttcttg gcaatttcct tgtctgttgt cgtagatata taacatccgt catatagacc
     2161 tgtttcaatt tgatgggcgt aaactgcatt cttcacactt ggaccatgtg tagctttacc
     2221 atcatattta aacttaccat cataacgaat tgcaacttca gctttattac ctttaggttt
     2281 taactgtcca ttattttgct catcttgttg gcaactaatg cctctatata agaaatttcc
     2341 cataagttat taatttaaag gttgaattta acaccattac tcatcaatct caagcaatcc
     2401 aaacattatc agattgcttg agagggatgt tttattttat aaggtaatgc ggcggaagtc
     2461 tttcaatagc ttcgccaaga atacggtgaa gcgcataccg gccgcaccgt cgatgacgcg
     2521 gtggtcgaag gacaggctca acgggcacat caggcgcggg gcaaactctt tgccgttcca
     2581 aaccggtttg atttgggatt tgcacacgcc caagatggcg acttcgggag cgttcacaat
     2641 tggcgtgaag cctgtgccgc cgatgccgcc taagctggaa atggtaaagc acgcgccttg
     2701 catttcttgc ggtttgagct tgccttcacg ggcttttttg gacaattcgg tcaattcttg
     2761 gctgatttgt ttcaaacctt tttgatccac gtctttgatg acgggaacaa ccaagccgtt
     2821 cggcgtatcg gctgcgaaac cgatgttgaa gtagtttttc agcaccaggt tgtcgccgtc
     2881 cagagaagcg ttgaattcgg ggaaggcttt cagcgcggaa acggaggctt tgatgatgaa
     2941 cgccagagga gacagtttca cgccttcgcg ttcccattct ttgttcagtt gtttgcggaa
     3001 ttcttccagc tcggtcatat ctgcttcttc gtgtacggta acgtggggaa tcaccaccca
     3061 gttgcgagac aggttttgac cggagatttt cttaatgcgg gacaattctt taacttcgac
     3121 attgccgaat ttggagaagt ccactttagg ccacggcagc aagtccagac cgctgcccaa
     3181 agatgcgccg gctgcggcag gttttgccgc gccaccctgc attacggatt tcacaaaggc
     3241 tttgatgtcg tcgcccacga tgcggccttt caagccggta cctttgactt ggcccaaatc
     3301 cacgcccaat tcgcgcgcca gtttgcgtgc ggaagggccg gcgtgtgctt tggcgaaagc
     3361 ggcctcgtcg attttggcag cggcaggtgc agaaggtgca ggcgcggcgg cgggtgcggc
     3421 ggcagcagtc ggaggcggag cggcagccgg tgcggcagct tgagcggctt gagcaggggt
     3481 tgctgcggca gagccgacgg tttctacttc gataatgaca gagccttcgg atactttgtc
     3541 gccgactttt aagaatacgg ctttaacgac accggcagcg gtacaaggta cgtccattgt
     3601 cgctttatcg gtttccaaag taatcagcgt gtcgtcttcg gcaacggtgt cgcccacttt
     3661 gatttcaacg gcgatgacat ccacatcggt atggccgccg atatcgggaa cggctacttg
     3721 aacggttgca ccgcctgcgg caacgggtgc ggcagcaggt gcaggttgtg cttcggcagc
     3781 agccgaggcg gcttcggcag cggcggcacc ggtttcaacg gtcaaaatta cgccgccttc
     3841 ggagattttg tcgccgactt tgacttttac ttctttcacg acaccggcag catcggcagg
     3901 cacatccatc gtggctttgt cggtttccag tgtaatcagg gtgtcgtcaa cggcgatggt
     3961 gtcgcccgct ttaacttcta cggcgatgat gtcgacgttt tcgtgaccgc cgatatcggg
     4021 gactttgatt tctacgatac tcatttgagt tcctttaatc ttgttcagtt ggatgccgtc
     4081 tgaaagctgc ttcaggcggc atcggtacgg tttgtctgct caaaccattc gggcattcgg
     4141 ttttcagacg gcctgatgtc ggaatggggc aaacagaccg tctgaaacat caatcagcgt
     4201 ttccagctag gagctgaatc ggctttgatg ccgtatttct caatggcttg ttgaacggtt
     4261 tctttgctga ctttgccttg ttccgccaat gcgctcaatg ctgccacggc aacgttgtag
     4321 cgatccactt caaagaagcg gcgcaggttg gcgcggctgt cggagcggcc gaagccgtca
     4381 gtacccaaga cgtggtagtc gttcgggatg taggcgcgga tacggtcagc atagctgcgg
     4441 atatagtcgg tagcggcaat aaccggaccg tcatgacctt gcagttggga agtaacgaaa
     4501 ggtacttttt cggcttccag cggatgcagg cggttgaagc gttctacctc gacagcgtcg
     4561 cggtgcagca ggttgaagga tgggcaagac caaatgtctg cttccacgcc gaagtcggct
     4621 ttcagcagct cggcaccggc aatcacttct tgcaaaatcg taccggagcc catcaattga
     4681 actttcttgt cgcctttgcc gccggctttc agcaggtaca tacctttcaa gatgtcttgt
     4741 tccgcacctt cgggcatatc cggatgggtg tagttctcgt tcatcagggt gatgtagtag
     4801 aacacgtctt cattattggc atacatacgg cgcagaccgt cttgtacgat gacggcgact
     4861 tcgtattgga aagtcgggtc ataagatacg cagttcggaa tcaggtcggc ctggatgtgg
     4921 ctgtggccgt cttcgtgttg caggccttcg ccgttcagcg tcgtacggcc ggcagtaccg
     4981 cccagcagga agccgcgcgc gtgcatatcg cccgccgccc aagccaagtc gccgatacgt
     5041 tggaaaccga acatagaata gtaaatgtag aacggaatca tcgcgaagtt gctgttggcg
     5101 tagctggttg cagccgcaat ccagtcggcc atcgcgcccg gttcgttaat accttcttgc
     5161 aagatttgac cgtcaacgga ttctttatag aacatcagtt ggtctttgtc ttgaggggta
     5221 tattgctgac ctttcggatt ccaaataccg tattggcgga acataccttc catgccgaaa
     5281 gtacggcttt cgtcgggaac gataggtacg acgcgtttgc cgattttttt gtctttcagc
     5341 aaagtggaca ggatgcggac gaatgccatc gtggtcgaga actcgcgttc accgctggat
     5401 ttgagttgtg cgtcgaatgc tgacagctcg ggcacttcca atacttcctg cgtcggtttg
     5461 cgttgcggca ggtagccgcc caaagcatcg cggcgtgcgt gcaggtattt gtattcttcc
     5521 gtatcggggg caaaagtcag gtaaggcaga tcgccgcttt cgatttgttc gtcggtaacc
     5581 ggaatgtcaa agcggtcgcg gaattgtttc agggacgctt tgtccatttt tttggcttgg
     5641 tgggcaacgt tctgaccttc gccggatgcg cccataccgt aacctttaat ggttttcgcc
     5701 aagatgacgg taggtttgcc gtcggcatgg ttcgctgcgc ggtcgtaggc gttgtacact
     5761 ttttgcgggt cgtggccgcc gcggttcaat gcccagagtt gctcatcggt catatcggca
     5821 accaatgctt tcagttcggg cgtattgaag aagtgttcgc gcacatacgc gccgtctttg
     5881 gatttgtaag tttggtagtc gccgtccaaa cattcttcca tacgttggcg caggataccg
     5941 tctttgtctt tcgccaagag gcggtcccag cggcggcccc aaatgacttt gacgacattc
     6001 cagccggcgc cggcaaagtt gccttccaat tcttggatga ttttgccgtt gccgcgtacc
     6061 ggaccgtcca agcgttgcag gttgcagttg atgacgaaaa tcaggttgtc caagccttcg
     6121 cgtgcagcca gtgcgattgc accttgagat tcgggttcgt ccatttcgcc gtcgccgcag
     6181 aaacaccata ctttacggcc tttggttttt gccaaaccac gcgattccaa gtatttcagg
     6241 aaacgcgcct gataaatcgc catgatgggc cccaagccca tggatacggt cgggaactgc
     6301 caaaagtcgg gcaagaggtg ggggtgcgga taggaaggca gaccgtgtcc gtccacttct
     6361 tggcggaagt tgttcagctg gtcttcggtc agacggccct cgacgaatgc gcgtgcatag
     6421 atgcccgggg cgacgtgacc ttggaagaag accaaatcgc cttcttcgcc ttcgcctttg
     6481 gctttccaaa agtggttgaa accgacttcg tacatggtgg cggcagattg gaaagatgcg
     6541 atgtgcccac ccagttccaa atctttcttg ccggcgcgca atacgatggc ggcggcgttc
     6601 cagcgcacga atgcgcggat gcggtgttcg atgttttggt cgcccggaat gcctttttcg
     6661 ttttcaaccg aaacggtatt caaatacggg gtggtcgtgc cgtgtggcat acgtacgccc
     6721 ttgtcgcggc agtatttgac caggttttcc aagaggtatt gcgcgcgttc gccgccttca
     6781 tattcgagga cggagcttaa cgcgtccagc cactcttggg tttcgatagg gtcaacatcg
     6841 tgtaattggg tggacatagt atctatcctt tatgttgagt gtttaatcgg cagggagggt
     6901 gtagccaacc cctttatttt cgtttgtgaa aaataataag caaaaacgaa aatttctaaa
     6961 tcaaaccgaa aaaaaataat ttaagtttaa tgttttcaaa aaattataaa agttttggaa
     7021 acaagggaaa acttgaaaaa aatgttcgat ctgctccaca cgaaatccta tcagcaatcg
     7081 ggtctgctgg caaataacca tgcctgtaaa agcaggtttt atactgaagt tggcgtttgg
     7141 taaggtcaag tctttttgat ttcgaaatat tctgtttgat tttggcgata ttttttcgga
     7201 attgtggtat atcgaatcat tatgtctctg tcggcaattt tcctattgta gaaaatctat
     7261 acacaagctt tgtggataac cgtgttgata agccacggct tgtgttaaaa aagtcttacg
     7321 caacaaatgt acgcttattt tgattaaaaa ataggcacat ttaaatctat aaaaatcatg
     7381 tgcttatgtt caatatgatg tttctggtca tgtttttgcc gtctgttatg ggatttgtcc
     7441 cgcgcttggg gcagggggtg aattttggag gttggtgcaa aacctgcttt tttcgcgtta
     7501 caatctgcac ccgatttact tttgaatttg cctgtttgga aaaatacttt tatgaaagcc
     7561 agccaattct ttatctctac tttaaaagaa gcccctgccg aagccgcgct tgccagccac
     7621 aagctgatga ttcgcgccgg cctgattaaa gccaacgcgt ccggccttta tacttggatg
     7681 ccgatggggc tgcgcgtgtt acgcaaagtc gaaaacgtcg tgcgcgagga aatggcgcgc
     7741 gcgggcagcg tggagctgct gatgccggtg gtgcagcctg ccgagctgtg gcaggaatcc
     7801 ggccgctggg aattttacgg taaagaactg ctgcgcctga aagaccgcca cgaccgcgat
     7861 ttctgcatgg gaccgacctg cgaggaagtc atcgccgaca tcgtgcgtaa agagatcaac
     7921 agctacaaac aactgccgaa aaacttttac cacatccaaa ccaaattccg tgacgaagtg
     7981 cgcccgcgct tcggtgtgat gcgtgcgcgc gagtttgtca tgaaagatgc ttattccttc
     8041 catgccgact acgcctcgct tcagacgacc tatgatgcca tgtatgacgc ttactgccgt
     8101 atcttcaccc gtctgggctt ggaattccgc cccgttgccg cagataccgg cagcatcggc
     8161 ggtaccggct ctcacgagtt tcaagtgttg gcggaaagcg gcgaagatgt gattgcatac
     8221 agcgacactt ccgattacgc cgccaatatc gagctggcac cgaccttgcc gcttaaaggc
     8281 gaacgcaccg ctgctcaggc agaactcgtc aaagtacaca cgccaaacgt caaaaccatt
     8341 gagtctttgg ttgaattcct gaatattccg gttgaacaaa ccctcaaatc catcgtggtt
     8401 gaaggcgaaa atgaaggcga aatcgtttta ttgctgttgc gcggcgacca tgagttcaac
     8461 gacatcaaag ccgaaaaact cgcaggtgtg aaatcgccat tgacgatggc aagccctgcc
     8521 gcgattgtcg aacagttcgg cgcaaacggc ggctcgctgg gtccggtcgg ctttgcaggt
     8581 aaagtttatg ccgatttcgc cactgaaaaa ggcgcggact gggtcatcgg tgcgaacgaa
     8641 gacggctacc actacaccgg cttcaacttc gggcgcgacg ctgccgagcc tgaatttgtc
     8701 gatttgcgcg atgtcgtcga aggtgacgaa agccccgacg ggcaaggccg tctgaaactg
     8761 gcgcgcggca tcgaagtcgg acacgttttc caattgcgcg acaaatacac ccaagccatg
     8821 aacgtaagct tccttgacaa caacggcaaa tcgcaaatca tggaaatggg ctgctacggt
     8881 atcggcatca cccgcgtcgt cgccgccgcc atcgagcaga ataacgacga aaaaggcatc
     8941 atctggacca aagcaatggc gccgtttgaa gtcgttatcg tgccgatgaa ctacaaaaaa
     9001 tcagacaccg tacgcgaagc cgccgacaaa atctatgccg aattgctggc ggcaggcgcg
     9061 gatgtgctgc tggatgaccg cgacgaacgc gcaggcgtat tgctgaacga ttccgagctg
     9121 ttgggcatcc cgcaccgcat cgtcatcggc gaccgcgcct tgaaagaagg caatgtcgaa
     9181 tacgccgaac gccgcaacaa cgaagcgcag gcagttgcaa tcggagaaat tgttgcgagt
     9241 gtaacagctt cattaaatgg gtaagcttgg tcaaatgccg tctgaaaata atttcagacg
     9301 gcatttttat ttggggagaa agtgaagtgg cctcagtatt tttagaagga gaagcagtcg
     9361 aggtcggcaa tattttctgt atcggcagga actatgccgc acatattgaa gagttaaaaa
     9421 acgaaatacc ttccgaacct gtggtgttta tgaagccgtc aggcagcatt ttgaatagcg
     9481 gcggtacaat cttactgccc gagttcagta gggatgtgca gtttgagtgc gaactcgtgc
     9541 tgctggtcgg taaggattca gacggcatgg gagagggtga agatattttg ggatgtgtgg
     9601 cggggtacgg tgtgggtttg gatttgaccg cgcgagacat ccaatgccat ctgaaggcgc
     9661 aggggctgcc ctggctgaag gcgaaagggt ttaggcattc cgcgtgcgtg tcggattttg
     9721 tggcggcagg cagaatcagc aatcccgaga aggtgttgtt ttcattaaaa caaaacggcg
     9781 tattgaaaca gcgcggggat accggtttga tgatttaccc gattcgggaa attttacaca
     9841 aactggcggc ggattacggt ttggggaaag gggatttggt gtttaccgga acgccctccg
     9901 gcgtgggcgc aatcggtgcg ggggacaatt tggcattgga gctggacggc ttggtgcgcg
     9961 cctctttcac ggtagggtgt taaccgcgcc gcttaaccct cacgcccgtt gaaacattcc
    10021 gcaggaccgc cctcgaaaaa accgtgcaag atggcttgcg ccgccacttg gtcgagcacc
    10081 gatttgcgtt ttttgccgaa gacctgtgct tccgaaagca ggctttcggc atagacggac
    10141 gacagccgtt cgtcaaccca atagacgggg agattgaacc tgccgttcag cctgcgtccg
    10201 aacttgcgcg acaggtgcgt catttcatgt ttcgtgccgt cggtatgcac gggcagtccg
    10261 acgacaaaat aacgcggctg ccattcttga accagcttgg cgattgccgc gaacttttca
    10321 tcgttgctgc cgccggtaac ggtgctcaaa ggatgggata gccctaattc cgcgtctcct
    10381 tgtgccacgc cgatacgcgc ttcgccgaag tcgaatgcca gtgccgttcc ttttggaatt
    10441 ttatgcatgg ccggctcccg aaaacagggc gagcgggtcg atgccgagtt tggcgaatgc
    10501 ggcggcgtaa cggtgttcgt aggggatgtc gaacaggatg tgttcgtcgg cgggaacagt
    10561 cagccacgca ttgtcggcaa gttcgcgttc gagctggcct tttccccagc ttgaatagcc
    10621 tatgctgatc aaggctttgt caaccgcacc ttcgcgtgaa atattttcaa tcacgtctcg
    10681 ggaagaagtt agcgcgatat tgtctgaaac gccgatactg ctttgccagt tgccgatcgg
    10741 ggtatgcacg acataacctc gctcgacttg caccggcccg cccatcatca cgctgtcgtg
    10801 ctgcatccgc atggggatgt ttttgccggt ggcggaaaaa atcatgtcca tcgtaatcgg
    10861 agagggtttg ttgatggcga tgccgagtgc gccgtcttca tcgtgtttgc agatatagac
    10921 gaccgattgt gaaaaaaacg cgtcttccat atcgggcatg gcgacgagaa agtggttgga
    10981 gaggttcata tcagcgtttg tcgggatttt ctatcgggtt ggaaatcatg caggcgccgg
    11041 caagcaggtt gccggaagta tcggcttgca ccccatcggt ttgcggtatg ccgccgccga
    11101 aacaggaaac cgcgcttaag gaatttttcg gcgagccttg gatgatttta tcgctgtaca
    11161 ccaaataggc gaaggttttg cgtttggggt cgtaataacg gacaatctgc cggctcttga
    11221 acgcgaagct cgcaccgtgt ttgaaaactt ctttcggttt gcgcacggcg gtttcgtcaa
    11281 aagaaatcga agatgccgtc tgaacgcacg aaaccgatgc gtcggacgcg tcctcttcca
    11341 aattgaccat ttccttcaag ccgccttttt ttgcatacga aatataacag gcaacccctt
    11401 gaacgtcggg atcgtcgaat ccttccactt cgatacggtc gtttttgccc agcatgttga
    11461 aaacggtact tgcccgtccg attttatcgg tttcgccgct gccgcaggca gtcagcagga
    11521 cggcggcaga cagcagtagc agtctgttca tcaaaatttc cttatcggtt gaatccccgt
    11581 tttccgtgaa ggcggcaggg cttgctttgt ttggaggggc gcacgttccc aaaatcgggc
    11641 aacatacggt atgcggctta atgtgctacc ctgtgtgtcg gaacacaata tccgttgcgg
    11701 cattgaaaat atgacgcaca atataaggct gtttgagccg attgcaagta tccgcgcaaa
    11761 gaaggcgcaa gattgggcaa acggcatttt cagacggcat aactgacagt ataatccaaa
    11821 cgtccggctg ctttcgtgca gccttcagta ttatccaact ttttccgaag gtcgaaccat
    11881 gcgctacaaa ccccttctgc ttgccctgat gctcgttttt tccacgcccg ccgttgccgc
    11941 ccacgacgcg gcacacaacc gttccgccga agtgaaaaaa cagacgaaga acaaaaaaga
    12001 acagcccgaa gcggcggaag gcaaaaaaga aaaaggcaaa aatggcgcag tgaaagataa
    12061 aaaaacaggc ggcaaagagg cggcaaaaga gggcaaagag tccaaaaaaa ccgccaaaaa
    12121 ccgcaaagaa gcagagaagg aggcgacatc caggcagtct gcgcgcaaag gacgcgaagg
    12181 ggataagaaa tcgaaggcgg aacacaaaaa ggcacatggc aagcccgtgt ccggatccaa
    12241 agaaaaaaac gcaaaaacac agcctgaaaa caagcaaggc aaaaaagagg caaaaggaca
    12301 gggcaatccg cgcaagggcg gcaaggcgga aaaagacact gtttctgcaa ataaaaaagt
    12361 ccgttccgac aagaacggca aagcagtgaa acaggacaaa aaatacaggg aagagaaaaa
    12421 tgccaaaacc gattccgacg aattgaaagc cgccgttgcc gctgccacca atgatgtcga
    12481 aaacaaaaaa gccctgctca aacaaagcga aggaatgctg cttcatgtca gcaattccct
    12541 caaacagctt caggaagagc gtatccgcca agaacgtatc cgccaagagc gtatccgtca
    12601 ggcgcgcggc aaccttgctt ccgtcaaccg caaacagcgc gaggcttggg acaagttcca
    12661 aaaactcaat accgagctga accgtttgaa aacggaagtc gccgctacga aagcgcagat
    12721 ttcccgtttc gtatcgggga actataaaaa cagccagccg aatgcggttg ccctgttcct
    12781 gaaaaacgcc gaaccgggtc agaaaaaccg ctttttgcgt tatacgcgtt atgtaaacgc
    12841 ctccaatcgg gaagttgtca aggatttgga aaaacagcag aaggctttgg cggtacaaga
    12901 gcagaaaatc aacaatgagc ttgcccgttt gaagaaaatt caggcaaacg tgcaatccct
    12961 gctgaaaaaa cagggtgtaa ccgatgcggc ggaacagacg gaaagccgca gacagaatgc
    13021 caaaatcgcc aaagatgccc gaaaactgct ggaacagaaa gggaacgagc agcagctgaa
    13081 caagctcttg agcaatttgg agaagaaaaa ggccgaacac cgcattcagg atgcggaagc
    13141 aaaaagaaaa ttggctgaag ccagactggc ggcagccgaa aaagccagaa aagaagcggc
    13201 acagcagaag gctgaagcac gacgtgcgga aatgtccaac ctgaccgccg aagacaggaa
    13261 catccaagcg ccttcggtta tgggtatcgg cagtgccgac ggtttcagcc gcatgcaggg
    13321 acgtttgaaa aaaccggttg acggtgtgcc gaccggactt ttcgggcaga accggagcgg
    13381 cggcgatgtt tggaaaggcg tgttctattc cactgcaccg gcaacggttg aaagcattgc
    13441 gccgggaacg gtaagctatg cggacgagtt ggacggctac ggcaaagtgg tcgtggtcga
    13501 tcacggcgag aactacatca gcatctatgc cggtttgagc gaaatttccg tcggcaaggg
    13561 ttatatggtc gcggcaggaa gcaaaatcgg ctcgagcggg tcgctgccgg acggggaaga
    13621 ggggctttac ctgcaaatac gttatcaagg tcaggtattg aacccttcga gctggatacg
    13681 ttgatcggat tgccgaaacg gtaggcttta tgcaaatcgt gccgatatgg tgcgctgttc
    13741 cgtgaagttt gccgtacctg tattttccgg tttttggaaa tcaagagaga aagttaaaga
    13801 agaatgtcga aacctgtttt taagaaaatc gcactttata ctttgggtgc aatcagcggc
    13861 gtggccgtca gtctggcggt gcagggtttt gccgccgaga aggacaggcg ggataacgaa
    13921 gtcctgccgg tgcaatccat ccgcacaatg gcggaggttt acggtcaaat caaggcaaac
    13981 tactatcagg acaaacccga tgccgatttg tttgaaggtg cgatgaaggg tatggtggcc
    14041 ggtttggatc cgcattccga atatatggat aaaaaaggtt atgccgagat aaaggagtcc
    14101 accagcggcg aatttggcgg cttggggatg gaaatcgggc aggaagacgg atttgtcaaa
    14161 gtggtttcgc cgattgagga cacgcctgcg gaacgggcgg gggtgaaaag cggcgatttc
    14221 attgtgaaaa tcgataatgt ttcgacacgc ggcatgacgg tcagcgaagc ggtgaagaaa
    14281 atgcggggca agccgggtac gaagattact ttgacgctgt cgcgcaaaaa tgccgacaag
    14341 ccgatagtcg tcaacctgac ccgtgccatt attaaagtga aaagcgtccg ccatcacctg
    14401 atcgaacccg attacggcta tatccgcgtg tcgcagttcc aagagcggac ggtcgaaagc
    14461 gtcaataccg ccgcaaaaga gctggtaaag gaaaataaag gaaaaccgct caaggggctg
    14521 gtgttggatt tgcgcgacga ccccggcggg cttttgactg gcgcggtcgg cgtgtcggcg
    14581 gcatttctgc cgtctgaagc agtcgtcgtc agcaccaagg gacgcgacgg caaagaccgc
    14641 atggtactga aagccattcc tgaagattat gtgtacggga tgggcggcga ttcgttggcg
    14701 ggcattcctg ccgagttgaa aaccataccg atgacggtat tggtcaattc cggttcggct
    14761 tccgcgtcgg agattgtcgc aggtgcattg caggatcata aacgcgcggt catcgtcggt
    14821 acgcagagct tcggcaaagg ttcggttcag actttgattc ctttgtccaa cggcagcgcg
    14881 gtcaagctga caacggcact gtattatacg ccgaacgacc gttctattca ggcgcagggg
    14941 attgttcccg atgtcgaagt aaaagataag gaacgcattt ttgaaagccg cgaggcggat
    15001 ttggtcggac acatcggcaa tcctttgggc ggcgaggatg tgaacagtga aacccttgcc
    15061 gtgccgcttg aaaaagatgc ggataagccc gctgtaaaag aaaaaggtaa aaagaaaaag
    15121 gacgaggatt tgtcttcaag gcggattccc aaccctgcca aagacgacca gttgcggaaa
    15181 gctttggatt tagtcaagtc gcccgagcag tggcagaagt ctttggggct ggcggcgaaa
    15241 aagccggttt caaataaaga taagaaagat aaaaaagata agaagtaggt tttgacgatg
    15301 ccgtctgaaa ggatttcaga cggcatatta tgtttgaaag gacaggttat gagcgatttt
    15361 ttaccggaat ttgaaccgtg cggcatttat agtggattaa caaaaatcag gacaaggcga
    15421 cgaagccgca gacagtacaa atagtacggc aaggcgaggc aacgccgtac tggtttttgt
    15481 taatccacta tacttgggta gcgattgggt ttcagacggc atacattcag gatttcaccg
    15541 ccttatgccg tctgaaagac gtttccgacc catccctact acacgctttg tccgcaaacg
    15601 catcacaggc aagattatgg aagtcattcg ataccccgat tcccctttca aactccacca
    15661 acccttcccg cccgcaggcg accagcctac cgctattgcc gacttgctcg aagggctttc
    15721 agacggcctt gcctaccaaa ccctgctcgg cgtaaccggt tcgggcaaaa cctacaccat
    15781 ggcgaacgtc atcgcccaaa gcggccgacc cgccatcatc atggcgcaca acaaaaccct
    15841 tgccgcccag ctttatgccg aaatgcgcga gtttttccct gaaaacgcgg tggaatattt
    15901 cgtctcctac tacgactatt accaacccga agcctatgtg cccagccgcg acctgttcat
    15961 tgaaaaagac agcgcgatca acgaacatat cgagcagatg cgcctttccg ccaccaagaa
    16021 cctgatgacg cgcgacgacg tgattatcgt cgccaccgtg tccgccatct acggtatcgg
    16081 cgacccgacc gagtatcagc aaatggtgtt gtccgttaaa gaaggcgaca ccattgagca
    16141 gcgcgacatc atcgccacgc tcgtttccat gcagtacgag cgcggcgatt tggactttaa
    16201 acgcggcagc ttccgcgtgc gtggcgacgt gattgacgtg taccccgccg aaagctccga
    16261 aaacgccttg cgcatcagcc tgtttgacga cgaaatcgac cgcctcgata tgttcgaccc
    16321 gctttcaggc agccttatcc agcgcgtcgg ccgctacacc gtcttcccgt ccagccatta
    16381 cgttaccccg cgcgataccg tcttgcgcgc ctgcgagtcc atcaaagaag aattacgtga
    16441 acgcattgat ttctttacca aagaaaaccg tcccgtcgaa caacaacgca tcgaacagcg
    16501 cacccgcttt gacctcgaaa tgctctacga aatgggcttc tgcaaaggca tcgaaaatta
    16561 ctcccggcac ttctccggca aaaaagaagg cgaaccgccg cccacgctga tggactacct
    16621 gcctgacaac gccatcatgt tcatcgacga aagccacgtt accgtcaccc aaatcggcgg
    16681 catgtacaaa ggcgacgcat cgcgcaagca aaacctcgtc gactacggct tccgcctgcc
    16741 ttccgcccgc gacaaccgcc cgctcaaatt ccatgaattt gaaaaagtca tgccgcaaac
    16801 tgtcttcgtt tccgccactc cagccaaata cgaagaagaa cacgccggac aagtggtcga
    16861 acaagtcgtc cgccccacag ggctggtcga cccccaaatc atcatccgcc ccgtcgccac
    16921 ccaagtcgac gacttaatga gcgaaatcaa cgaccgcatc caaaaaggcg aacgcgttct
    16981 cgttaccacc ctcaccaaac gcatggcgga gcaactcacc gactattaca gcgaactcgg
    17041 catcaaagtg cgctacctgc acagcgacat tgataccgta gagcgcgttg aaatcattag
    17101 agatttacgg ctcggcctgt ttgacgtact cgtcggcatc aacctcttgc gcgaaggcct
    17161 cgatatcccc gaagtctccc tcgtcgccat cctcgacgcc gacaaagaag gcttcctgcg
    17221 ctcacaccgc agcctgattc aaaccatagg ccgcgccgcg cgcaacgtga acggcgtcgc
    17281 gattctgtac gccgacaaaa tcaccgactc tatgaaagcc gccatcgacg aaaccgaacg
    17341 ccgccgtgaa aaacaaatta aattcaacga agaacacggc atcgtgccgc aacagattaa
    17401 aaaacaggtc aaagacatca ttgacggcgt gtaccacgaa gaagacggtg gtaaaggccg
    17461 tagacaaggc aaaaacaagg ttaaagtcag cgaaattcac aacgaagaag acgcggttaa
    17521 agaaatcgcc aaactggaaa aagccatgca gcaggcggct agggatttac agtttgaaga
    17581 ggcggctgta ctgagggata ggatacgggg gattaaggaa gggttgttgt ttgggagtga
    17641 gtaataaaag cacttgaaag tttagttgat gaccacaatg ttcttaaaag atctggagaa
    17701 acaaaagatg tggagaaaca tcgtaacttg atcaattaca ttctgaggac tgaaaatgac
    17761 atattctttt cgtgtagaaa atataaatga tatatctgat attcctgaaa atcatttgga
    17821 aatagttaga gctgctctgc ctagtaatag aagtaatgcc ttatctcgtg agccagatgg
    17881 ggcacaagct gtttttgttt atgaaattga tggtgaattt agagctaagt tatttgaccg
    17941 cataaaagat acagacagtt atttacaagc aacagaggat aattactcta atgaccctcg
    18001 cagtatagga gtagtatacg ttttggagca acagcaaggg tagcctgagg gccgcacaaa
    18061 ctctcaagct gacgtgcagt atgtgtgata cgcacgcatg cgttttttcg ttcaaaccat
    18121 tgttttaaac cttgtatcga aacatcactc gctgctttat cttgaaaaaa tgccgtctgt
    18181 aagcaaacat gcttcaaaca gcattttttt atccggcggt ttgttagccc atccgtttga
    18241 ataccaagtc ccaaacgccg tgcccgaggc gtttgccgcg cgcttcgaat ttggtttcgg
    18301 ggcggtagtc cggggtgggg gcgtagtctg ccgccgtatt ttgcaggctg tcgaagctac
    18361 tgaggacttc aagcatctgc cgtgcatatt cttcccagtc tgtcgccagg tggatatagc
    18421 cgccggtttt gagtttgggc agcagtttgg cgatgaaggg ggcttgtatc agacggcgtt
    18481 tgtggtggcg ttttttgtgc cacgggtcgg ggaagaatat gtggatgccg tcgagcgagc
    18541 cgtcttgcag catattttcg acaacttcta cggcatcgtg ccgcatcacg cggatatttt
    18601 ccaaatggtt ttcgtctatg agtttgagca ggttgcctac gccgggaccg tgtacgtcga
    18661 tggcgaggaa gtcggtttcg ggcaggcggc gggcgatttc tgcggttgcc gtccccatac
    18721 cgaagcctat ttcgaggatt ttgggtcggc tgcttccgaa acgggcatca agatcggccg
    18781 gtgttgcttg gtagtcgatg ccgaagctgt cccataaggt atcgatggcg cgttgctgcg
    18841 cggcggtcat atggctttgg cggaggacga agctgcggat gctgcggggg tgtcctgctt
    18901 gacggtcgtt tggtgtttgg ttttcggcgg tgtctgtcat aatgcttccg tgcgcttgtg
    18961 caatcaggat gccgtctgaa tgtgaaaggc ctgttcggac ggcgggaaag gaaaggggcg
    19021 attatacctg tttcgtttcg gcagggtagg tgtaacgctg ttcagacggc attttgccac
    19081 tgttgcaaca gttcttgcag gacgcttttt tgcccgtatc cgttgtttat ggcttcttgc
    19141 aggtagcggc aggctttttc tttgtccggc ccgacaccgt gtccgctgta atggatgcag
    19201 gcgaggttgt agaacgccat actgtctttt ttcgcggcgg ctttttcaaa ccatttgcgt
    19261 gcttcattgt agtcggcggt cataccctgt ccgaagtaat acatgctgcc cagattggtt
    19321 tgggctttgc tgtgtccgag ttctgcggct tctgtgtaga gtttgagcgc ggctgcgtat
    19381 tcgggcgcgc attgtaatcc gtaatgcagg gcttgggctt tttgataaag ctgttctgcc
    19441 ctttgccgcc taatggcgga atctttaatg ccgccgtatt ggtcaggatg gtttaacgcg
    19501 ctgtcggata tgagtttctg ataggcggta aggctgccgg cttcggctgc ctgccggtaa
    19561 taatgccggg ctttttccgt atcgggggct atgcccaaac cgtagcggca gatgtcgccc
    19621 agtatccgca aaccttcggg atggcgttcg gcggcggctg cttcggcgtg atgcagggca
    19681 agtttgtagt ttctttccgg atgcttgcct tgcaggtaga tgtcggcaag tgcggcatgg
    19741 gcatcggggt cattttcctg tgcggcctgt tgaaaccggt aaacggcttt atcgggagcc
    19801 gtaggtagga tgagctcggc aagcggggtg taggcgggaa tgtatccttg ttgtgcggcg
    19861 gcgcgcaaat gattgatagc ttgggtcgtg tcggttggtg taccttgtcc gaaacggtat
    19921 tgaaggccga gctgccattg tgccgccgcc aagccttgtg ctgcagcctc gcggtagaga
    19981 aggtgtgccg tttcaaaatc cggcggcgtg ccgtaccggc agtattgggc gagcaggtat
    20041 tgcccgtggg gattgttgtt ttttgtgctt gtttttgccc attccaaggc ttcttcaaaa
    20101 tgtcgggacg catacagcag gtacatcagg cggacttgcg cttcggacag acctgcttct
    20161 gccagaggcc tgtaaagcgg tatcagggtt tcagggggcg cgccttccaa ttcttggata
    20221 tatgccagat tgtaacgcgc gtagggatgc ccctgttcgg cggcgatgcg gagcatttcc
    20281 ataccttgtt cgcggtcttg gtaggggctg tccgcatcca tcaggtgttt tgccaactcg
    20341 aatgcagcgg cgggatgacc tgccaaggcc gcttctgtca gcagtcggcg gacttcggta
    20401 aagttatatt gtttttgaga caggcgttct aaggcctgcc gatagggtaa gtcgggattg
    20461 ttcatatcag gtcgttttcc gttttgtcga attatcgtta ttcgttatga agttaggttt
    20521 atatcggcac gccgacctac tgtatcatat tttttacaga cggtttttgc tttggcggca
    20581 tactaatgca gatgattgta aatggtttcc gccaatgcct tgctgatgcc ttccacttgt
    20641 tccaaatcct cgcggctggc ggcaatcacg ccgcgcaggc cgccgaagcg ggtgagcagg
    20701 gcttggcggc gtttgctgcc tacgccgggg atgtcgctta aggaggaggt aacgcgggct
    20761 ttgtcgcgtt ttttgcggtg gccggtaatg gcgaaacggt gcgattcatc gcgtacggtt
    20821 tgcaataaat gcaaggccgg gctgtgtggg ggcaggcgga agacttcgcc ggtaaaaggc
    20881 agtatgagct cctccatacc ggctttgcgc tccgggcctt tggcaatgcc gaccaaaggg
    20941 atgtgcagcc cgagttcttc ccataccgat acggctacgc cgatttgccc tttgccgccg
    21001 tcaatcaaca cggcatccgg ccatttgacg gtctcgccgt tggcttcggc ttcctgcatt
    21061 ttgccgtaac ggcgcgtcaa cacttcgcgc atggcggcgt agtcgtcgcc gggtttggcg
    21121 gtcgtgatgt tgtagcggcg gtattgcgag ggctggatgt tttgctcatc gtacacgacg
    21181 caggaggcga tggtggcttc gccttgggtg tggctgatgt cgaagcattc gaggcggttg
    21241 aggccgtctg aatccatacc gaggattttg gcgagttcgt cgatgcggtg ttgctggttg
    21301 ctttgttgca ggcggcgttg tgcaatcgcc atttgtgcgt tttgttccgc cattttcaac
    21361 caaactttgc gttcgcctat ggtcttggtg acaaattgca tttgtttgcc gtgttcgcct
    21421 tccaaagcct ctttcagcgc atcgggaacg gaaaagttgc taataatgat gtcgggtttg
    21481 cttttgccca aataatgttg ggcgacgaag gcttcggcgt aatcttgtcc gtttggctcg
    21541 ggatcgtttt tggtgtcggg gaagaaactt ttgtctccga cgtgccgccc accgcgtatg
    21601 ctgacccagt gtacgcagac gatgccgtct gaaaccgcca gcgcgagcaa atcgatatcg
    21661 ttggggttgt tcgggttttt gctgtcgatg aactggttgc tctgcataat gccgagtgct
    21721 tggatttggt cgcggtagcg ggcggcttct tcaaattgca gattcgcggc ggcagtttgc
    21781 attttgtgtt gcagggtgcg ggtcagttcg tcggttttgc cattgagaaa agtggcggct
    21841 tgacgcacgc tgtcgcggta gtcttcttcg ctgatgtgtc cgacgcaggg cgcggtgcag
    21901 cgtttgattt gatacagcag gcaagggcgg tcacgatgct cgaatacgct gtcttcgcag
    21961 gtacgcagca taaagacttt ttgcaacact tgaatgctgt cgcgcacggc gttgctgttg
    22021 ggatacgggc cgaagtattg gtttggcttt ttcagcgtgc cgcggtaata cgccatctgc
    22081 ggatattgat ggccgctgag catcaaataa ggatagcttt tgtcgtcgcg gaaaaggata
    22141 ttgtatttcg gcgacaaggc tttgatgagg ttgttttcga gaatcagggc ttcggcttcg
    22201 gaacgcgtga tggtggtttc gatatgatga acctgtttta ccatcaatgc gatgcgcggc
    22261 gaatggtcgt ttttttggaa atagccggac acgcgccgct tgaggttgac cgctttgccg
    22321 acgtataaga ctttgccgcc ttcgtcaaaa aaacggtaca cgcccggcag cttgggcagg
    22381 tttttgagga agagcgggat atcgaattgt tccggagaac ggttttctgt attcatatca
    22441 attatttgaa aaacggcttc agacggcata tcggcagcgg cagccttatc cgttcggaaa
    22501 agctgtcggg tggttatatg cggtcaatcg atttttaccc gtttcaggcg caaggcgttg
    22561 ctcaacacgg aaaccgagct tgccgccatt gccgcgcctg cgatgacagg gtttaaaaaa
    22621 ccgagcgcgg caagcggaat gcccaaaata ttgtagaaga aggcgaaaaa caggttttgc
    22681 ttgatgtttc tcaacgtcgc ccgcgatacc gatagggcat cggcgagttg gttgaccgaa
    22741 tgttgcatca gcgtggcgga tgcggtgtgt tcggcaacgt ccgcgccgcc tttcatggcg
    22801 aagctaacgt tggcggcggc gagcgcaggt gcgtcgttga tgccgtctcc gaccatcgcc
    22861 acggttttgc cggcggcttt gagtttctgc acttcggcgg ctttgtcgcg cggactcata
    22921 ttaccgaagg cgtgtgcgat gcccagttgt ttggcgacgt actcgaccgt gccttggttg
    22981 tcgccgctca taatatagac atcgatattg tgttttttca gacggcctat ggcttcggcg
    23041 gtatcggctt tcaacgcgtc ggcaagtgcg aatgcgccga tgggtttgtt atcgactgag
    23101 accgcaacaa tgcttgcaat atcccaaacg ccgtctaaaa acttcggcaa ggccagttcg
    23161 gcaaattcgg ctttgcctgc tttcaccaaa cccacgcctt ccacttcggc ggtaatgcct
    23221 gcgccgacaa cggtttgtgc gttttgtgcg gcgggaatgt ccaaaccgcg cgcttgggcg
    23281 gcggagacga tggcacgggc gagcggatgg gcggcgtttt gttcgacggc ggcggcgatg
    23341 cggtacaaag cgtcttcgtc aaagccgctg tcgggaacgc aataaacggc ggcaacctgc
    23401 gggctgcctt cggtcagcgt accggttttg tccaacacga cggcatcgac gtgggcggct
    23461 tcctccattg ctgccgcgtc tttaaaccaa ataccgtgtt tcaccgcttt gccaatgccg
    23521 accataatcg cggcgggtgt agccagaccg agcgcacacg ggcaggcaat caccaaaacg
    23581 gcgacggcgt gcatcagcgc aaccgtccaa tcgcccttaa tcagccaagt aacaataaaa
    23641 gtcaacaacg cgatgcccac gacggcaggc acgaacaccg ccgccgcctt gtccgccacg
    23701 cgcgcaatcg gtgctttact gccttgcgct tcagagagtg cgttcatcat gtcgccgagc
    23761 agggtttggc tgccgagctg cgcggcgcgg tacaccacgc tgccttcagt catcagcgcg
    23821 cccgccaata ctttgccgcc tgcctttttc tcttcgggat tggattcgcc ggtaagatgg
    23881 ctttcgtccg cccagccgct gccgctttct atgatgccgt cggcggcaat gcgttcgccg
    23941 tgattggcgc ggattaggtc gccgatttgc acttggtcga tgggtagctg ccgccattcg
    24001 ccatcgcgtt gcacgttgac ttgggttggc gtgagtttga gcagcaagcc caagctgttc
    24061 aggctggatt ttttggtgcg gtgttccaaa aatttaccca gtgacacaaa accaatcacc
    24121 attatgccta cttcaaaata cacatgcgcc ataccgtacg ccgcgtgcgg gctgaaaaac
    24181 agcatataga cggaatacag gtaaatcgag accgtgccga tggtaacgag tacgtccata
    24241 ttcgccagcc cgcctttaat gctcgcccac gcgcttttgt aaaatggcac cgccagccaa
    24301 agctgcacca cactcgccaa tgcaaactgc aacaagggcg acaacatcca atcatgccgt
    24361 gtccaattca gcccttttag catcatccct accataccga taaggaacgg gatattgatg
    24421 gccagcaaaa gccacaacct ccagccgata tggtgttctg cttcgggttg cggcaatgta
    24481 tcttccgtct tttccttcgc gccgtaaccg gttttctcaa tgattttggc aatgtcggca
    24541 gcggaagttt tgctgtcgtc aaacgtaacc tgcgcctcct cgctggcgaa gttcactccc
    24601 gctgatttga caaagtcttt tttgttcaac actttttcaa tgcgcgaagc acatgcctga
    24661 caggtcatgc cttcgatttg gaaacggatt ttttgttgca taagcgtgat tcctgctttg
    24721 atattcagac ggcagttaag gctgcgggat gccgtctgag cgccggtatg gtcgaaattt
    24781 ctcaaacgcg ccgataattc gagtagaatc cgaaaacttg acacagcaaa ggaaaaacaa
    24841 tgtcccaaca ccgcaaactg attattttgg gttccggccc cgccggatac accgccgccg
    24901 tctatgccgc gcgtgccaat ttaaaccccg tcattattac aggtatcgcg caaggcgggc
    24961 aactgatgac caccaccgaa gtggacaact ggcctgccga tgccgacggc gtgcaagggc
    25021 cggaattgat ggcgcggttt ctcgcccacg ccgaacgttt cggaacggaa atcatttttg
    25081 accaaatcaa cgccgtcgat ctgcaaaaac gcccgtttac actcaaaggc gatatgggcg
    25141 agtacacttg cgatgccctg attgtcgcaa caggcgcgtc cgccaaatac ctcggtttgc
    25201 cgagtgagga agcgtttgca ggaaaaggcg tttccgcttg tgccacctgc gacggtttct
    25261 tctacaaaaa ccaagatgtt gccgtagttg gcggcggcaa tacggcagtt gaagaggcac
    25321 tctaccttgc caatatcgcc aaaaccgtta ccctgatcca ccgccgcagc gagttccgtg
    25381 ccgaaaaaat catgatcgac aaactgatga aacgcgtgga agagggcaaa atcatcctca
    25441 agctggaaag caacctgcaa gaagtactgg gcgacgatcg gggcgtaaac ggcgcattat
    25501 taaaaaacaa cgacggttct gagcaacaaa ttgccgtcag cggcattttt atcgccatcg
    25561 ggcacaagcc gaataccgat attttcaaag gtcagttgga aatggacgaa gccggttacc
    25621 tgaaaaccaa aggcggcacg gcggacaatg tcggcgcaac caatatcgaa ggcgtatggg
    25681 cggcgggcga cgtaaaagac catacctacc gtcaggcgat taccagcgcg gcttccggct
    25741 gtcaggcggc tttggacgcg gaacgctggc tgggcagcca aaacatttga atgccgtctg
    25801 aaaccgaaat tttcggcgga atgcaagttt ttcgcaccaa atccttgatt ccgtcggcaa
    25861 accccaatat aatgcccgtt ctttgaacct tgccttctgc tttcgcatgg cagaaggctg
    25921 tttttaccat ccataaggag ataacatgcg tcattacgag atcgtgttta tcgttcatcc
    25981 tgatcaaagc gagcaagtgc ccgctatggt tgaacgttac aaaaccatga ttgctgaagc
    26041 aaacggtaag attcaccgtt tggaagattg gggtcgccgc caactggctt acccgattaa
    26101 caaaatccat aaagcccatt acgttttgat gaacatcgaa accactcccg aagtggttga
    26161 agagctggaa accgcattcc gcttcaatga tgccgtattg cgccatctaa ccatcaaaac
    26221 caaacacgcc gttaccgaag catcccctat gttgggtggt gaaaaggcta agaacctgtt
    26281 gagcggtgcg tctgaagaag cggtcgccca ataattggga ttcaataatc ttgtttcgct
    26341 tgccgcgtta attgaaaagg ttttccctat tcgatatacg cctgccggaa tccctgtttt
    26401 agatattatt ttaaagcaca aatcgtggca ggaggaaaac gggcagcaat gccttgtcca
    26461 attggaaatt ccggcacgga ttttaggcag gcaggcggaa gagtggcagt atcggcaagg
    26521 tgtatatgtt cacgtcgaag gttttttagc tcaaaaaagc agacgttccc ttatgccgat
    26581 gctcaggata caaaatattc aagaatataa aggttaaacg acaatggctc gtcaatcatt
    26641 caaacgtaga aaattctgcc gtttcacggc tgaaaaaatc caagaagtcg attacaaaca
    26701 agttgatttg ctgaaagact ttatctctga aaacggtaaa atcattcctg cacgcatcac
    26761 aggaacgaag gcattctacc aacgccaatt ggctgttgcc gtaaaacgcg cacgcttcct
    26821 ggctctgctg ccttacaccg accaacacaa ataattttgg agattgaatc atgcaaatta
    26881 ttctgttaga aaaaatcggc ggtctgggca acttgggcga catcgtaacc gttaaaaacg
    26941 gctacgcccg caactttcta attcccgcag gtaaggcaaa acgtgcgacc gaagcgaata
    27001 tgaaagagtt tgaagcacgc cgtgccgaat tggaagccaa acaggctgaa attttggcag
    27061 atgcccgagt ccgtcaggaa aaattggacg gtcaaaccgt taccgttgct caaaaagccg
    27121 gtgtggacgg ccgcctgttc ggttccgtta ccaatgccga cattgctgct gcaatcgttg
    27181 ctgccggcat cgaagccgtg aaagcaaatg tacgtctgcc gaacggtcct ctgaaagccg
    27241 ttggcgagta cgaagtggaa gtggctttgc acactgatgc cgttgctaaa attaccgttg
    27301 ccgtcgttgc cgcaaccgag taattgaaat tatcagatgc cgtctgaaac cctgccgatt
    27361 gtcggaatgt ggtttcagac ggtatttgtt ttgttttccg tatattctgc aaaaatcggg
    27421 atggttgttt ttatagtgga ttaaatttaa accagtacag cgttgcctcg ccttgccttg
    27481 ccttgccgtg ctatctgtat tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc
    27541 cactatattt gcctgtggtc tttgcaggaa tagtttgtta acgaaatttg atgcataaaa
    27601 tgcgccaaaa aatattggct gtgttttaat atagtgttga taagcgtacc tggcttcaat
    27661 atactcaata ggatagatct cttcctaacc gaaaaaataa ttgtcttttc ccttgcaaat
    27721 aattgaaatc aacagattac taaaacacag ccaaaatatt caattgacaa aaacttttct
    27781 gattttgaga aatctgtgaa aatcagggga ttatttattt tgaaaatggt atcgggcata
    27841 aaatttggct gtgtttcaat atggtgctga taacatactt tgtttcaaaa tgttcaatag
    27901 gataacttcc tgattgaccg aaaaataatg gccttttccc ttgcaagcag ttgaaatcaa
    27961 cagattgtca aaatacatcc taaaaattta ataacttata tatatgattg caaaggttgc
    28021 ggcttgtata ccggtcatta tggcggaaat accgtttggg aggcagccgc atcgcgtgga
    28081 atcggcacgg caggcgatgc gccgatgcct tcgggcgtga aggcggggca atcggcagtt
    28141 tggaaaaagg caatggaatc tgtgggcaga agaagttaag ggtaaaagtc gggcgcacgg
    28201 tcaggttgtc tgtccttttg tcggaacagg agactgggca gctttgtcct gattaattgc
    28261 ttgtgcaaac ggtaaaaagg ttttttcgtg tgttttaaag caggttgtac agcatttttc
    28321 cggcaatcaa aagcaacata atgccgaagg attttttgag tttggcagaa gaaagtttgt
    28381 gggcggtttt gacaccgagc ggggcaaagg caatggttgc cgcgctgagg acggcgacgg
    28441 cgggcaggta aaggaagccc agtgaccctt cgggcaatcc tgcaatattc aggccgttga
    28501 gcagatacga tattgcgccg gagagtgcaa tcggccaggc aaggccggat gatgtgccga
    28561 tggctttatg ggcggggaag ccgcagtgga ttaagaaggg gacggaaagt gaaccgccgc
    28621 ctatgccgac ccagctcgac attgcgccga acagtgtgga aaccgcagtc agtccgggca
    28681 gtccgggcag cgggcgggat gccgtctgag ggtcggtatg cagtgttttg aatgcgacgg
    28741 cggttaaaaa caggatgaag aaaatttgaa gcccgaacgc ggggatatat tttgcggaga
    28801 gtgcgcccgt gaatacgccg aatatcatac ccggcatcat cgcaaatacg gttttccagt
    28861 cgaccgcctg ttttttgtgc tgccccaaca tactggaaaa ggcggtgaag accatgacgg
    28921 cgaaggatgt gccgacggcg aggtgttgcg cgtaaggatg ttgtgccaaa ccctgcaaat
    28981 caagtaccca taaaacgaca gggacaatca gcgtgccgcc gcctacgccg aacagaccgg
    29041 caataaaacc tgccgcactg cctacggcaa gcaggattaa gataatgtcc caatgccaca
    29101 tttaatccgc ctttctgtat tttttccaga atcggcgtgc cgccataatg tagccggaca
    29161 ggctgtatcc gagaaagaac aggaagagga cgagcgacgg ttcccaagtg accagaagca
    29221 gcagtaagac ggcaagcagc attccgacaa aggggacttg tctgcggatg ttgatttctt
    29281 taaaactcca aaaagggatt tggacaatca tcgacaggcc ggcaaacagt gtgataccca
    29341 atgcccacca gtggacggcg gggaattttt cgacgctgtg gttgacccaa atcagcccga
    29401 caatcagcgc ggcagcagtc ggactgggca cgccgataaa ccagcgtttg tccaccttgc
    29461 cgatgagcgt attgaacagc gcaaggcgca gggcggcgca ggcgcagtag atgaaggcga
    29521 cggaataacc gattttgccg aactgccaaa gttgccattt gtaggcaata agcgcggggg
    29581 caacgccgaa gctgaccatg tcggcaaggc tgtcgagctg ctccccgaac gcgctttggc
    29641 tgttggtcag ccgcgccacg cgcccgtcca taccgtccaa aagcatggaa atgaatacgg
    29701 caatagcggc ggtttcataa cgtccgtgca tggattgggt gattgcgtaa aacgcggaaa
    29761 acagcgcggc gatagtaaag gaattgggca gcaggtagat actgttttga cgcagggagc
    29821 gttgcggggt gttggtcggg gtttccatag ctgaagtgtc tcacggacgt ggagaatcga
    29881 taggggatta taacccaaac cattatcgaa caatacgccc tttaggatga agccctcgga
    29941 ggcggtttgt ttttctgatg gcggatgctt tgcggggcgg tttgactctg cctgccgccg
    30001 ggtgtgttgt ttcggatttt tgccggatgg atgttgttgc cgggcatttc tgggcttgcc
    30061 ggaaggtatc ggggggaggg catcggaata tgcggcgcgc cgttcaaacc ggggcggcgt
    30121 ttccctattt tcggggagtt tgcaggcttc aacaaattga aagatatttt ttgcatttcc
    30181 gaccgtatgc tgattttgaa acgggataaa ttataggaca aggtttttcg cccgccgcaa
    30241 aggttggccg caatgccgtc tgaaaacaac ggcttgccgc taaggcggcg gtatgcggat
    30301 ttgcgctgct gaaatcggtt tgccataccc ggcggtgcaa tctgcgggga gggataagcc
    30361 gataaggcgg gacttatcct gttttttgtt tataatgcaa cagaatttgg acgaaccgtc
    30421 gggaatttat catgatgaaa aagatactgg ccgtatcggc actatgcctg atgactgcgg
    30481 cggcacgggc tgccgatact tacggctatc tcgccgtttg gcagaatccg cagaatgcgg
    30541 acgatgtttt gcaggttaaa accacaaaag aagattcgac gaaaagcgaa gcgttttccg
    30601 agttggaagc cttctgcaaa ggtcaggaca cgcttgcggg cattgccgaa gacgagccga
    30661 ccggatgccg gtcggtcgtg tcgctgaaca atacctgtgt cgcgctggca tacccgaaag
    30721 ccttgggcgc gatgcgcgtt gaaaacgccg tcgtgattac ttctccgcgt tttacgagcg
    30781 ttcatcaggt cgcactcaac cagtgcatca aaaaatacgg cgtacaggga caatgcggct
    30841 tggaaacagt gtattgcaca tcttcttctt attacggcgg aactgtgcgc tctttgattc
    30901 aaaatctcaa ataaaacgga aaatgccgtc tgaaagatgt tcagacggca tttctatatc
    30961 gacggtcagg attctttcgg atcgggcagc aggctgttca acataatggc aagtacggcg
    31021 cacaagccca cgccggcaaa gctgaagctg cccaatttga gcgtcatgcc gccgatgccc
    31081 gtggtcagta ccgagctgac gatgaccagg tttttcggca gcatcaaatc gactttggca
    31141 tcaatcagcg ttttcacgcc caaagaagca atcgtgccga acagcagcag cataatgccg
    31201 cccattactg gcatcggaat ggaagccaaa aacgcattga atttgccgaa aaacgccatg
    31261 cagacggcaa aaaccgccgt ccaagtcatg atgacggggt tgctgttttt ggtaatcatc
    31321 accgcacccg ttacttcgcc gtaggtcgta accggcgggc cgccgatcag acccgcaacg
    31381 catacgccca aaccgtcgcc tgcaagggtt ttgtccaagc ccgggtcttt cgtatagtct
    31441 ttccccgtca cattgccgat tgccatgatg ccgccgatgt gttcgatggc gggggcgacg
    31501 gcaacgggca gcataaacag tgcagcctgc cagttgatct gaggcgtttc aaaatgggga
    31561 acggcgaacc agggcgcgtg tgcaatgctt gccgtgtcca ccagtcccat cagcagtgcc
    31621 aaaacataac ccgaagcgac accgatcaag atgggaatca gcttcatcat cctgctgccg
    31681 aaaaccgata cgatggcggt aacggcaaag gtaaagccgg aaagaatcag cgaatcggta
    31741 tagtcgatga cctgtttgcc gtccgcctga cccattgcca tgctgcttgc cgccacggca
    31801 acagacagac cgatgaccat gatgacgggg ccgatgacga ccggaggcag cagtttgtgt
    31861 accgctgcca gtccgcgcca acggatcagc gcggcaaaca caaaatacat aaagccggcg
    31921 gcaaacagtc cgaacatggt ggaaggcagc ccccattcgc cgacggagta gataatcggt
    31981 gcgataaagg caaacgaaga accaagaaaa atcggcactt tgcgtttggt tgtgatttgg
    32041 aacagcagcg ttcccaagcc tgcgcccaaa agcgcaagag ccggattcag accggtcagc
    32101 aggggaacca gcaccattgc gccgaatgcc acaaataaaa tctgcgcgcc ggaaacggca
    32161 agtttcagtt ggttcatacc atcttctttc aatcggttca aacggcacgg attatactgg
    32221 tttgccgagg gtctgcgtaa aaatacatat ggcgggcaac agatatgccg tctgaaaggc
    32281 aatgtctgtc tgttgcagga tgaaagcagg agaaatggag tataatccga cactttccat
    32341 attcaatccg caagccgtcc ggcttcctta tttcagacgg cattttccgg taatacggca
    32401 tgacagaaaa atcccataaa aaaacagcaa aaggcagggc gggaagcccg tccccaacgt
    32461 ccgcacgcaa caagaaagcc gacaacggcg cacggggcaa taaagtttcc gagcggctca
    32521 aggcggtcaa agagttgcag aaaaccgaaa ccaaaaaggc gcgtcccgaa catgtcgtca
    32581 accttatcgg cgacgcactg tggctgatgg gtttggcggt aaccttgtat ttggcgattt
    32641 ccctgatcag tttcgatatg ggcgatccgt cttggtcgca cagttcgccg gttgtggaag
    32701 atgtcgccaa ttggggcgga ctgttcggcg cgtatgttgc cgatgtcggc tattatcttt
    32761 tcggctggtc gttctggtgg tggatagcgg ctgcctgcgt catgctgtat aaaaattttc
    32821 gtctgcacgc aaaacagacg gaaaacgagg catacaacca caaaatcgct gccgccgcgc
    32881 tgtttgtcct gacggtcttc agccccgtct tggagtattt tgtgctgggc ggaaaatatg
    32941 ccgactccct gcctgtcgga gcaggcggta tggtcggcat acgcgtcggc gcagtgtttg
    33001 cgtggctgct ggggaaatcg ggcagcctgc tgattatctt ggttgttctg ctgttgtcgt
    33061 tgtccctgct ggtgcagatt tcatggctgg aatttttgaa cggtgcgggc agggcggttc
    33121 aaaaccgcct gagtgcctta tccggcaagg tcatggcttt aggaaaacgc cggccgaata
    33181 gcaaaacaga cggtgtcgat acccaaaata cacggcgcat ggtaaaagaa gccaagaata
    33241 ttacggccaa acccgttgcc ttgcccgaag gcagcagcag caaccgcaaa tccgtcgcgg
    33301 tttccgtcgc gccgtcgccc aaaattcagg tttctctgtt tgaagatgac gaacctcggc
    33361 aggcgggcga ataccacaag cctacattga acctattgcg gattcctgac agcgaacccg
    33421 tcagcatcaa tcccgccgaa ttggagcgca ctgccgaact gatcgaatcc aaactggcag
    33481 aattcggcat cggcgtacaa gtcgtatccg ccacatccgg tcccgtcatc acgcgctacg
    33541 aaatcgaacc cgcgcaaggt gttaaaggca gccaaattgt tgccttgtcg aaagatttgg
    33601 cacgctctat gtcgctgcaa tccgtgcgta tcgtcgaaac catcgcaggt aaaaacacga
    33661 tgggcatcga gttgcccaac gacaaacgcc aagacgtgat gttgagcgaa atcttgtcct
    33721 cgcccgtgtt tgccgaagcc aaatccaagc tgaccgtcgc gctgggcaaa gacattgccg
    33781 gtacccccgt tgtcggcgac ttggcgaaaa tgccgcacct tttggtcgcc ggtatgactg
    33841 gttcgggcaa gtccgtcggc gtgaacggca tgattatgtc tatgcttttc aaagctacgc
    33901 ccgacgaagt ccgctttatc atgatagacc cgaaaatgct tgagttgagc atttacgacg
    33961 gcattccgca cctgctctgt cccgtcgtga ccgatatgcg cgaagcgggg caggcgttga
    34021 actggtgcgt cgccgaaatg gaaaaacgct accgcctgct ttcccacgcc ggtgtacgca
    34081 atctggaagg cttcaaccaa aaagtcgaag ccgcaaaagc ggcaggcaag ccgctgctca
    34141 atccgttcag cctgaacccc gacgagcccg agcctttgga aaaactgccg atgattgtgg
    34201 tcgttatcga cgaacttgcc gacctgatga tgaccgaacg caaagccgtc gagcagcaaa
    34261 tcgcccgtct cgcccaaaaa gcgcgcgccg ccggtatcca tatgattgtc gccacccaac
    34321 gtcccagtgt cgatgtcgtt actggcctga ttaaagccaa catcccgacg cgtatggcgt
    34381 ttaccgtgca aagcaaaatc gacagccgta ccatcctcga ccaaatgggc gcggacgaac
    34441 tgctcaaata tggcgattcg ctgttcctcc agcccggcag tgccgaaccg acccgcctgc
    34501 aaggcgcgtt tgtttcagac gacgaagtac atcaagtcgt caactacgtc aaatcgcaag
    34561 ccccagccga ctatattgaa ggtctgctca gcggcgaggc cgcgctggaa actgccaata
    34621 tcgttaatcc gaatgcagac agcgacgaat tgttcgatca ggcagtcgcc tatgttttgg
    34681 aaagcaaaaa aacctccatt tcgtctttgc agcggcagtt gcgtatcggc tataaccgcg
    34741 cggcaaacct gatggaggca ctggaaaatg cgggtgtcgt ttctcccacc gacctcaacg
    34801 gcagccgtaa aattttggcg cacaaggacc atttgtagcc cgtattgcaa aatgccgtct
    34861 gaacggcgga attggcgttt cagacggcat attatgtttc aggcgaaaca ttgtgatata
    34921 cttgccagct aaaatttccc ctttgcggca atgcggttca aatatcgtac cgttgcgctg
    34981 tttgcttccc catgtaggga agaaagttta tcattttatc aacacaacaa atttaagggc
    35041 ttatgatgag cgtaactgtt gaaactttag aaaatctgga acgcaaagta gtgttgtccc
    35101 tgccttggtc cgaaatcaac gcagaaaccg ataaaaaact gaaacaaacc caacgccgtg
    35161 caaaaatcga cggtttccgt ccgggtaaag cacctttaaa aatgattgcc caaatgtacg
    35221 gtgcgagcgc acaaaacgac gtgatcaacg agctggtgca acgccgcttc tacgatgttg
    35281 ccgttgccca agagttgaaa gtggcaggct tcccccgttt tgaaggcgtt gaagaacaag
    35341 acgataaaga gtctttcaaa gttgccgcca tttttgaagt gttccccgaa gtcgttatcg
    35401 gcgatttgtc tgcacaagaa gttgaaaaag taaccgcttc cgtcggtgat gccgaagtgg
    35461 accaaaccgt agaaatcctg cgcaaacaac gcacccgctt caaccatgtc gaacgcgaag
    35521 cccgaaacgg cgaccgcgtc atcattgact ttgaaggcaa aatcgacggc gaaccttttg
    35581 ccggcggcgc atccaaaaac tacgccttcg tattgggcgc aagtcaaatg ctgcctgaat
    35641 ttgaagccgg cgtagtcggc atgaaggctg gcgaaagtaa agacgttacc gtcaatttcc
    35701 ctgaagacta ccacggtaaa gacgttgccg gtaaaactgc cgtgttcacc attacgctga
    35761 acaacgtttc cgaagcgact ctgcctgaag tcgatgcaga ttttgcaaaa gccttgggta
    35821 ttgcggatgg cgacgttgcc aaaatgcgcg aagaagtgca gaaaaacgta agccgcgaag
    35881 tggaacgccg cgtaaacgaa caaaccaaag aatccgtaat gaacgcgctg ctcaaagccg
    35941 tagagctgaa agcacctgtt gctttggtca atgaagaagc cgcacgcttg gcaaacgaaa
    36001 tgaaacaaaa ttttgttaac caaggtatgg ctgatgctgc caacttggat ctgcctttgg
    36061 atatgttcaa agaacaagcc gaacgccgcg tatctttagg tctgatttta gccaaactgg
    36121 ttgacgaaaa caaactggaa ccgactgaag agcaaatcaa agccgttgtt gccaactttg
    36181 cagaaagcta cgaagatcct caagaagtga ttgactggta ctacgcagat ccttcccgcc
    36241 tgcaagcccc gacttctttg gcggtagaaa gcaacgtcgt tgatttcgtt ttgggtaaag
    36301 ccaaagtaaa cgaaaaagct ttgtcttttg acgaagtgat gggcgcgcaa gcctgattat
    36361 cctgaaaatg ccgtcttagg gcagtttttg aaagcaccga agccgctttt aaagcccgct
    36421 ttggtgcttt tacatcatga aaggagacga aatgtctttt gataactatc ttgtccctac
    36481 cgttatcgag cagagcggtc gcggtgagcg tgcattcgat atctattctc ggcttttgaa
    36541 agagcgcatc gtattcttgg tcggcccggt aaccgacgag tccgccaatc tggtggttgc
    36601 ccaactgttg tttttggaaa gtgagaatcc ggataaggat attttcttct atattaactc
    36661 gccaggcggt tcggtaacgg ccggtatgtc gatttacgac accatgaatt ttatcaagcc
    36721 cgatgtatcg actttgtgct tggggcaggc agcaagtatg ggcgcgttct tattgtcggc
    36781 aggcgaaaaa ggcaaacgtt tcgccctgcc caacagccgg attatgattc accagccttt
    36841 aatcagcggc ggcttgggcg gtcaggcatc cgatattgaa attcacgcac gcgaactgtt
    36901 gaaaatcaaa gaaaaactca accgcctgat ggcgaaacat tgcggccgcg atttggcaga
    36961 tttggagcgc gacaccgacc gtgataattt catgtctgcc gaagaagcaa aagaatatgg
    37021 tttgatcgac caagttttgg aaaaccgcgc ttctttgcag ctttaaacct tgtgaagtag
    37081 acaaatgccg tctgaaaaca gttcagacgg catttgtctt atagggactg tattgggatg
    37141 attttgttct ggttgcgttt ggagccgttt tcgccgtagt tggttttgac atactcttca
    37201 acaatagcca aaaattccgt tgcaatgttg tcgcctttca gcgttacttt gcgttcacca
    37261 tctacataaa caggcgcgac gggtgtttcc cccgtaccgg gcaggctgat gccgatgtcg
    37321 gccaatttgc tttctccggg cccattgaca acgcagccca ttacggcaac gttcagggat
    37381 tcaaccccgg gataaagggt acgccatata gacatttttt ggcgcaggta attttgaaca
    37441 tcttgtgcca gctcttgaaa tacggtactg gtggtacgcc cgcagccggg gcaggcggta
    37501 accatcggcg taaacgaacg caatcccata gtctgtaaaa tctcttgccc gacgacgacc
    37561 tcctgagtac gcgggctgcc aggttccgga gtcagtgaaa tgcggatggt gtcgccgatt
    37621 ccttcttgaa gcaagacgga taatgccgcc gttgatgcga caatgccttt gctgcccata
    37681 ccggcttcgg tcaaacccaa atgcagcgga taggcgcaac ggctgcccag ttcgcggtaa
    37741 acctgaatca aatcctgaac cgcgctgact ttgcacgaca ggatgatttt gtcttcgggc
    37801 agtcccaata gaacggcttt ttcggcagat tccaaagcgg agacaatcag tgcttccttc
    37861 gtcacttctt cgggcggttt cggcgcggaa gaagcgaggt tggcatccat catacgtttg
    37921 gcgaggcttt gatccaaaga accccagttt acgccgatgc ggacggcttt atcgttttca
    37981 gcagcggtcc gaatcataaa ggcaaatttt tcatcgcctt ttacgccttt gccgacattg
    38041 ccgggattga tgcggtattt ggacaatgct ttgccgcatt cgggaaattc cgccaacagg
    38101 cgttcgccgt tgaagtggaa atcgccgata agtggtgtgg catagcccat atcgtccaag
    38161 cggcggcgga tttcggcaac tttggacgcg gcttcggggc tgttgacggt aatacgcacc
    38221 atttcggatc cggcatcgct caattcctta atctgcaatg cggtggcttt tgcatcggca
    38281 gtgtcggtgt tggtcataga ttggataacg acgggtgctt ctgaaccgac ggtaatatga
    38341 tcgatgcgga cttgatgcgt cttgcggcgt tggagtgtgt tcatatggtt tgaatctgtt
    38401 tattgaccga tgaggacggt ttgcaattct tccgagtagg ggaaattcgc ctgcaattgt
    38461 gcttcatatt cgtatgccgc ctgtgcgttg ccgagggctt tggcaatttt ccagcctagc
    38521 agcaaatcat cggcctgaag gacttctacc ctgctttggt attttttaaa gtagtaatcg
    38581 gcatcgccca actgcccggc cagcattttg gtgcgcgcca gttctttaaa tgcgggtggg
    38641 aactgcggct gggcggcgag ggaacgtttc aaataggctt ccgccaatcc gaattgcccc
    38701 tgttttgcgc tgcatatgcc tttattcagg ttggcaatat aaggggtcgg gtaggtgggg
    38761 tcggccaggg ctttgtcgaa atatgccata gattcggcag ggcggttgag cctgccgcac
    38821 aggaaccagc cgtagttgtt gttgatttcg gcactgtcgg gtttgatgga gagggcttgc
    38881 cggaaacttt cctgcgcctt gtcgttaact ttcaggtatt ggtagatttc ggcacggacc
    38941 agccaggcaa gctcgttttt agggtccgat ttcagggcgt cttcaatact tgccgtcgcc
    39001 tgacggtagt cctgaccgcg catatattcc attgccaact gggttttgat attggaaacc
    39061 tgattggctt tttctgcccg cgaggggcgg taggaagtgc tgcacgcgcc caaggcaagg
    39121 acaagtaata aagagattcg tttggatggc ttaaaaggca taattacccc tgttgtccga
    39181 ttaaaatctg ctgccatttt tgttggcggc gcgttttatc ctgaacctgc cccgccaact
    39241 gtccgcaggc ggcatcgatg tcgtcgccgc gcgtttttcg tacggtaacg acaaatcctg
    39301 cctgctgcaa aatatcgcgg aacacacgga tgttctcatt gctggagcgt tcgtatccgg
    39361 agtttgggaa gggattgaac ggaatcagat tgaacttgca gggaacatct gtgaccagtt
    39421 cgatcagttc gcgcgcatgt tgcgccttat cgtttattcc gtccaacatg acgtattcga
    39481 aagtgatgaa atccctgggt gctttgacca gatagcgttg gcatgcggcc atcaattctt
    39541 tcaagggata ttttttgttc aacggtacga tttggttgcg gacttcgtca ttggaagcgt
    39601 ggagggaaac cgccaaagcc accggcatga catcgcgcaa cctgtccatt tggggaacca
    39661 tacccgaagt ggaaacggtt acgcggcggc ggctcaaacc gtagccgtgg tcgtccagca
    39721 tgatgcttaa ggcggtaacg acattgtcga agttcgccat cggctcgccc atgcccatca
    39781 tgacgacgtt ggaaataacg cgctcgtttt tcggtgtaac gcccatggct ttgtttgccc
    39841 accacaattg cccgatgatt tcggcagagg tcaggttgcg gttgaagccc tgccggccgg
    39901 tcgaacaaaa tgtacattcc aaagcgcagc cgacttgaga ggaaatgcag agcgtgccgc
    39961 gatccgattc ggggatgaag acggtttcca cgccgttgcc cgtaccgaca tccaaaagcc
    40021 attttcgagt gccgtctgaa gatttttgag acatcatcag cttgggaatt tcgatgcctg
    40081 cctgttcgtt cagtttatgg cgcaacgatt ttgccaaatc ggtcatttcg tcaaaatttt
    40141 gcgcgccgga ttggtgcatc caacgcataa cctgtttggc acggaaaggt ttttcgccca
    40201 tatcggcaaa atgtcgggtc agcccttgaa ggtcgtagtt gagcagattg gttttcatgt
    40261 gtttgtgttt ttcttaaatc aaggcttcaa ataaaaaatt gcagggcagg caagtttgat
    40321 ttgaagcctt ttgcaacaga gttttcagac ggcatggata tatttatgcc gtctgaagag
    40381 gagttgggcg gtgtattgta tcaacggggg cagatttcgg tttggctgaa aaagtaggca
    40441 atttccaaag cggcattttc cacgctgtcg gaaccgtgta cggcattaat gctgaccgaa
    40501 gtggcaaagt ccgcgcgtat cgtgccttcg gcggcttcgg aaggattagt tgcacccatc
    40561 agttcgcggt ttttcaggac ggcgttttca ccctctaata cctgaatcat aaccggaccg
    40621 ccggtcataa attcaaccaa tccggcgtag aaggggcggt ctttatgaac cgcataaaat
    40681 tcttgcgcct ctttgagagt aagctgcttc attttggcgg caacgatttt cagaccgttc
    40741 tcctcaaagc ggctgtatat tttgccgata acatttttgc cgacggcatc gggtttgatg
    40801 atggagatgg tacgttcaat cgccatgcta tatccttatt tttacttaag aagaatcaaa
    40861 tcggtattct atcaaaaaaa taacttatcc gcctgaagcc ttgggagata gggtcaggtg
    40921 cggctgttcc aaataaactt cggactgcat ttcgaccatc cggctggcgg tgcgggtaaa
    40981 ttcttcggca aaatcgcctt ccgtatagat atggttgaca tctaccgcgc cggtggcaca
    41041 cagtttgacc cggaaatcgt agagtacgtc aatcagccaa gtcagccgcc gcgcctccgc
    41101 cttttcttgc ggtgagagtt gttccaaacc tgaaataaaa accatttcat aatgttcggc
    41161 caaatacaga tagtcggact gtgagcgggg gccgaagcac agtgcgcgga aatcaaacca
    41221 tatggcacgg ccggactcgg ctttgtgggg aatctcccga ccgtggatgg tgctgatgcc
    41281 ggggttcaaa tcggtaatgc ctgtcatttc tttgaacagt tttgccagtt ttgcctcatt
    41341 ttcttcattg gcaggcgtaa agaaaatctc ggcggggcgg agggtacgca gtcggtagtc
    41401 ttcaccgccg tcaacgttta agacggtcag gctggactcg atgagcgcga ttgtgggaag
    41461 aaaactgctc cggttttgac cttgcgggta gagttcggaa ggcgcgtagt ttgaagtcgc
    41521 caccaaaaca acgccctcgt taagcaggtt ttccagcaga cggcctaaaa tcattgcatc
    41581 cgcaatatcg ctgacatgaa attcgtcaaa acataatacg cgggtttctt tggcaatctc
    41641 ggcggcaacg gatttcaacg ggttgctttc gcttttcagg gttttcagcc tctggtggat
    41701 ttctgccata aaggcatgaa agtggacgcg gcgtttgcgg cggtaaggga ggcagccgaa
    41761 aaaagcgtcc atcagaaagc ttttgccgcg tccgaccccg ccatagaaat aaagcccttt
    41821 ggggacttgc ggggaacgca aactcctgcc taaaaaacgg tttcttttgc gtttgaacat
    41881 catcaattcg gtccaaagcc gatcgaggtg ttcgatggca gctgcctgcg cgtcgtcgcg
    41941 gatgaagttg ggcagttgtg aggcagcctg ataccaagtc agcgggctgt gattttcaaa
    42001 cggcggggct ttaaaaagtt ggtctctatt actcatattt aaccttgtta tacttcttgc
    42061 ggctaagaaa gaaaatgctt accgtcggta ttataaatga tttccttcaa acttggcatt
    42121 ttccgtggaa aagaataatg acgcgcacaa gggggtataa gcaaaggcgg cgtttcaata
    42181 tggtgcagat atgagctcaa atgcctttta tagtagatta aatttaaacc agtacagcgt
    42241 tgcctcgcct tgccgtacta tctgtactgt ctgcggcttc gtcgccttgt cctgattttt
    42301 gttaatccac tataatccgt agagtcggct ccttccggct cagacggcat acgtttatgc
    42361 cggcacttct taccgccctc gtgccggcta tcccaaaact gctttgaaca ctgaacgctc
    42421 tttggtttac ttcaaaaacg cggcgtgata agcaatatgc tcgccaataa aactggcgat
    42481 gaagtaatag ctgtgatcgt agcctttatg gaaacgcaca tcgaccggct ggtttgccgc
    42541 acggcaggtt tcgataaaat cttcggtacg caattgtgtc ggcaaaaact catcttccaa
    42601 gccttgatcg atgcgcatac cttgcacttt atagccttgt tgaatgagtg agttagcatc
    42661 atattgctgc catttttcac ggtctttccc taaataagca gtaaaggctt tttctcccca
    42721 cggcacgagg cttggcgata aaataggcga aaaggcagaa acactttgat aacgttcctg
    42781 attccgcagc gccaatacca atgcgccgtg tccgcccatt gaatgtccca taatggaacg
    42841 tttgccgttg gtaggaaagt gtttctcaat cagacggggc agctcgttca aaatgtaatc
    42901 atacatttga taattcgccg cccaaggctg ttcggtcgca ttcaaataaa agcctgcact
    42961 ctgtcctaaa tcgtaagcat catcgttcgg cacttgctct ccgcgagggc tggtatcgga
    43021 ggccaccaca attacttgat gttctgccgc ataacgctga aagcctgatt tggtaatgaa
    43081 attttgttcc gtacacgtca agccggaaag ccaataaatt acgccaagag gtcgattttc
    43141 tagattattt ggcaaataga cggcaaattt catttcgcat tgaagcgttt gggcattgtg
    43201 tgcccaaact tgttgcgaac cgccaaaaat ttgatgttgt tcaatcagtt tcatcgcata
    43261 ccttagtagt gaataacggc gcggatcgat ttaccttcgt gcattaactc aaaggcttca
    43321 ttgatttgat cgagtgtcat tgtgtgggtt acaaacggtt ctaactcaat gtcgcctttc
    43381 attgaatctt ccaccatttt cggcagttca gagcgacctt tcacaccacc aaatgcagag
    43441 cctttccaaa cacgacctgt taccaactgg aacggacgcg ttgaaatttc ttgtcctgca
    43501 cctgctacgc cgatgataat ggattgaccc caaccacggt gtgcactttc taatgcctga
    43561 cgcattacgt ttacgttgcc gatacattca aaggtatggt caatgcccca tttattaatg
    43621 tctaacaaca catctttgat cggtttatcg taatcgttcg ggttcaaaca atccgttgca
    43681 ccgaactgtt ttgccaactc aaattttgat ggattggtat caatggcgat aatgcggccg
    43741 gctttggctt gacgcgcacc ttgcaccacc gccaaaccaa tcgcgcctaa gccaaacacg
    43801 gcaacagagt cgccttcttg cacttttgcc gtattatgta ccgcaccaat acctgtggta
    43861 acgccgcagc cgagcaaaca tacttgttca tggttggctt cagggttgat tttcgccagt
    43921 gaaacttcgg caacaacgga gtattcactg aaagtcgaac agcccatata gtgatagatt
    43981 ggctgacctt gataagaaaa acgcgtcgtg ccgtccggca ttaagccttt accttgtgta
    44041 tcacgcactg agacgcacaa gttggtttta cctgaacaac aaaactcaca ttcgccacat
    44101 tcggcggtgt aaagcggaat cacgtgatca cccggtttta cgcttgacac accttcgccc
    44161 acagcaacga ccacacccgc accttcgtgt ccaagcacca cagggaatac gccttcagga
    44221 tcgcttcctg ataacgtaaa cgcatcagta tggcacacgc cagtgtgggt attgcggatt
    44281 aacacctcgc ctttacgcgg catttctacg tcgatttcca caatttgtaa gggttggtta
    44341 ggggcgaatg ccaccgccgc acgagatttg atggttgaat cggcttgttt catttccatt
    44401 gtctttcctc ttaattcatt gacaaagttg ccttacctga gcaacatagc aacagtttac
    44461 actttagagt taactctaaa gcaagtgttt tttactgttt ttgtttagaa ggagggaaaa
    44521 tggcttatac cactgccaaa gccgccgaaa aaataggcat ctccgcccac accctacgtt
    44581 tttacgacaa agaaggcttg ttgcctaata tcggacgtga tgaatacggt aaccgctgtt
    44641 ttaccgataa cgatttgcaa tggttgggct tattgcaatg cttgaaaaat acggggatga
    44701 gcttaaaaga catcaaacgc tttgcggaat gtaccgtcat tggcgacgat accattgaag
    44761 aacgcctttc cttgtttgaa aatcaaatag aaaatgtgaa gtgtcaaatt gccgaattaa
    44821 aacgctattt agatttgctt gaatacaaat tggcgtttta ccaaaaagcg aaagcattag
    44881 gctcggtaaa agctgtaaat ttgccgcaaa ttcctgaaac ggcttagttt tagtgcaata
    44941 agcaggattt ttgtcctgtt cgtaatagaa atcaaaaatt tgttgtttaa aaagcggatt
    45001 gtaagcaat
//
LOCUS       DAWFJR010000004        44312 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWFJR010000004 DAWFJR010000000
VERSION     DAWFJR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 44312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44312
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.004"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(51..1220)
                     /locus_tag="WGR04_000177"
     CDS             complement(51..1220)
                     /locus_tag="WGR04_000177"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ0793818.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPSIAYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1403..1513)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1566..2462
                     /locus_tag="WGR04_000178"
     CDS             1566..2462
                     /locus_tag="WGR04_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ0793819.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2506..3570)
                     /gene="tsaD"
                     /locus_tag="WGR04_000179"
     CDS             complement(2506..3570)
                     /gene="tsaD"
                     /locus_tag="WGR04_000179"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ0793820.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGSMTVQIPTPTGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLKT
                     GREAGAFNVRPRWPLSEIVK"
     gene            complement(3687..4874)
                     /gene="ccsB"
                     /locus_tag="WGR04_000180"
     CDS             complement(3687..4874)
                     /gene="ccsB"
                     /locus_tag="WGR04_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ0793821.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4867..6819)
                     /locus_tag="WGR04_000181"
     CDS             complement(4867..6819)
                     /locus_tag="WGR04_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ0793822.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGSFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7096..7719)
                     /locus_tag="WGR04_000182"
     CDS             complement(7096..7719)
                     /locus_tag="WGR04_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ0793823.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7925..8554
                     /locus_tag="WGR04_000183"
     CDS             7925..8554
                     /locus_tag="WGR04_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ0793824.1"
                     /translation="MNLFQNAKFFTTINHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWLDAADAAASSPEEN"
     gene            complement(8675..11071)
                     /locus_tag="WGR04_000184"
     CDS             complement(8675..11071)
                     /locus_tag="WGR04_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ0793825.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11223..12338
                     /locus_tag="WGR04_000185"
     CDS             11223..12338
                     /locus_tag="WGR04_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ0793826.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12341..12940
                     /locus_tag="WGR04_000186"
     CDS             12341..12940
                     /locus_tag="WGR04_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ0793827.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12941..13588
                     /locus_tag="WGR04_000187"
     CDS             12941..13588
                     /locus_tag="WGR04_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ0793828.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13606..14151
                     /locus_tag="WGR04_000188"
     CDS             13606..14151
                     /locus_tag="WGR04_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ0793829.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14170..16455
                     /locus_tag="WGR04_000189"
     CDS             14170..16455
                     /locus_tag="WGR04_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ0793830.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQAAAPAKQQAAAPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
     gene            17417..17929
                     /locus_tag="WGR04_000190"
     CDS             17417..17929
                     /locus_tag="WGR04_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ0793831.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            18009..19088
                     /gene="aroB"
                     /locus_tag="WGR04_000191"
     CDS             18009..19088
                     /gene="aroB"
                     /locus_tag="WGR04_000191"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ0793832.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLETAKLPSAPPVFPFEKWLEHMSHDKKVSGGVMRFIGL
                     NRLGEAVITEITDTDILRRTLQPYL"
     gene            20055..20897
                     /locus_tag="WGR04_000192"
     CDS             20055..20897
                     /locus_tag="WGR04_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ0793833.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLVDFFPEQGEFFQPVGTTDSERAFCHILNRLRTRFVARPDDDTLFGAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDETWAQLAVDELVMFREGKIILNDRPRHPVYMSVEEGLETARAA
                     GVAV"
     gene            20927..21391
                     /gene="nrdR"
                     /locus_tag="WGR04_000193"
     CDS             20927..21391
                     /gene="nrdR"
                     /locus_tag="WGR04_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ0793834.1"
                     /translation="MKCPFCAHPDTRVADSRLMDERNAVRRRRYCPNCGKRFGTLETA
                     ELKMPAVIGTDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETARLTELRLYASGLRD
                     ISAAELVEIVLEELFGQSTEAAVRFAALHKRFDNPADFASWLAQAVKTGGKA"
     gene            21424..22533
                     /gene="ribD"
                     /locus_tag="WGR04_000194"
     CDS             21424..22533
                     /gene="ribD"
                     /locus_tag="WGR04_000194"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ0793835.1"
                     /translation="MFSDTDISMMENALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALHQAGEMAQGSTAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEEARADVQTLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSRLLTDGQSPTYIATLERDEDKLRPYRKHAHVRILMPSETAGGK
                     IDLNSLMRLLADEGIGELIVEAGSELTSAFLAEDLADEIVLYRSPKILGSGKDLFSLP
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNGNAF"
     gene            22728..24149
                     /locus_tag="WGR04_000195"
     CDS             22728..24149
                     /locus_tag="WGR04_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ0793836.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEANAPPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACAASFW
                     LFFVFKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGVWAAYLAG
                     CILRHRKDLHKLFHYLKKQGFPL"
     gene            24146..25222
                     /locus_tag="WGR04_000196"
     CDS             24146..25222
                     /locus_tag="WGR04_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0793837.1"
                     /translation="MKIVLTTSMAGLGGTETATVRLGRLLKRRGHDIILASSDGPFVG
                     EAQASGIRWQPVDFYRGGLAGYLKSTFAYARMLRREQPDIIDCQMARVVPACALAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGFPADRIVYAY
                     NALPPPPPEFPFWKTVKGCVMLGTLSRLDTVRAVHLMLDIFKKMVDRDMPVRLNVAGI
                     GEEMDNLKAQAERLGIDGKVTFLGGIRDLTAYFKDVDILVNTPHCIGDHGAGVGNNIL
                     EAGLYDTPVVTYDMAGISEMVVNGETGYCIPFGDEEAFIAAVDTLVRQPELREAMGKR
                     LHAHVAALCSDDEIYRTTMAAYTM"
     gene            25242..26414
                     /locus_tag="WGR04_000197"
     CDS             25242..26414
                     /locus_tag="WGR04_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0793838.1"
                     /translation="MNITIVAPYCSLPSEPHFNRFRYLAELLSQSHDVLLITSNFKHY
                     DKSFRRPEDAEAASQGRLKVMLLEESGYGKNVSLGRLASHHRFVRHFEKWLENCRPGE
                     QDIVYSAYPLIATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHNLLPFA
                     SRANQAYRYADALVAVSQTYLDRAKETNPNVPGEVVYIGADFPKLDAAPAKDFGDDKT
                     RFFYLGTLSYSYDVETVCKGVLKLLDAGENVELHIMGGGPDLDRLKQYACDDIKFYGY
                     IPYAEMMSVAKGCDIAINAIQSYSMASITNKLSDYMALQKPILNSQVNDEVAKVLTLL
                     PYANYRSGDVDGFVQAAKDILKRKNDPVQSEEIVRRFKRDVAYQKIVNLIERLAHE"
     gene            26407..27648
                     /locus_tag="WGR04_000198"
     CDS             26407..27648
                     /locus_tag="WGR04_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HEZ0793839.1"
                     /translation="MNKFFKRLFDIVASASGLIILSPVFLILVYLIRKNLGSPVFFIQ
                     ERPGKDGKPFKMIKFRSMRDALDSDGIPLPDSERLTPFGKKLRAASLDELPELWNVLK
                     GDMSLVGPRPLLMQYLPLYDNFQNRRHEMRPGITGWAQVNGRNAASWEERFACDIWYI
                     DHFSLCLDIKILLLTVKKVLIKEGISAQGEATMPTFTGKRKLAVVGAGGHGKVVAELA
                     AALGTYGEIVFLDDRAQGSVNGFSVIGTTLLLENSLSPEQYDIAVAVGNNHIRRQIAE
                     KAAALGFALPVLIHPDATVSPSATVGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHD
                     CLLDAFVHISPGAHLSGNTHIGEESWIGTGACSRQQIRIGSRATIGAGAVVVRDVSDG
                     ITVAGNPAKLLPRKNPETGTA"
     gene            27650..27766
                     /locus_tag="WGR04_000199"
                     /pseudo
     CDS             27650..27766
                     /locus_tag="WGR04_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            27891..29066
                     /locus_tag="WGR04_000200"
     CDS             27891..29066
                     /locus_tag="WGR04_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ0793840.1"
                     /translation="MLNTSLSPWPCFTQEEADAVSKVLLSNKVNYWTGSECRKFEKEF
                     AAFAGTRYAVALSNGTLALDVALKAIGIGAGNDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETIKAALTSNTKAIIVVHLAGMPAEMDGIMDLAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKALWSKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVNELNARKVPCYQGSCSEV
                     YLEKSFDNTPWRPKERLKNAVELGDTSLMFLVHPTLTDGEIAFCKEHIEAVLTEATR"
     gene            29114..31024
                     /gene="pglD"
                     /locus_tag="WGR04_000201"
     CDS             29114..31024
                     /gene="pglD"
                     /locus_tag="WGR04_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ0793841.1"
                     /translation="MTLETLLALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTALLTIGLFVRMGLYRAVTRFVSFHVLTTAFAGSIVSAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RLRIINKLEAYPCEVLTIPGMKDLMDGKLSIGTLKKISVSDLLGRDSVAPDDRLMNAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVRTFVLISTDKAVRPTNTMGAGKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPITLTHQDITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTANETMLPWRELSALLDRIRAACDRYDQQAIRTLLINAPTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            31080..32372
                     /locus_tag="WGR04_000202"
     CDS             31080..32372
                     /locus_tag="WGR04_000202"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ0793842.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHIEGQHFVSAKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAQEHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEILKAEGTLIIPGEHFFIGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(32611..33447)
                     /locus_tag="WGR04_000203"
     CDS             complement(32611..33447)
                     /locus_tag="WGR04_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ0793843.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLDLRLINGVEISVTWRGRTIHVVGLDFDERDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIRAGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGLLASAGSDFHRLNDFSGGILGACPELPENCRPVWEHFS
                     RH"
     gene            complement(33893..34102)
                     /locus_tag="WGR04_000204"
     CDS             complement(33893..34102)
                     /locus_tag="WGR04_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793844.1"
                     /translation="MDMTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWSEDENAV"
     gene            complement(34080..34313)
                     /locus_tag="WGR04_000205"
     CDS             complement(34080..34313)
                     /locus_tag="WGR04_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0793845.1"
                     /translation="MEAIVYLEDNEYIALCDLLKLVGLAESGGQAKALIAEGLVLRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            34845..38279
                     /gene="dnaE"
                     /locus_tag="WGR04_000206"
     CDS             34845..38279
                     /gene="dnaE"
                     /locus_tag="WGR04_000206"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ0793846.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPIGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     EAYVGKDRNVHHAELNPEWLDNGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPSQFFIPPETMAERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDYLVKLSNEGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLEKAMETEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKSTTGDIVDVGKIPLDDQVAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMNNADQKAANANQGGL
                     FDMMEDAIEPVRLVDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLDR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQTARERYARSLSLALAPHHDIGGLVQLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            38557..41802
                     /gene="cas9"
                     /locus_tag="WGR04_000207"
     CDS             38557..41802
                     /gene="cas9"
                     /locus_tag="WGR04_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEZ0793847.1"
                     /translation="MAAFKPNPINYILGLDIGIASVGWAMVEIDEEENPIRLIDLGVR
                     VFERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGL
                     IKSLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLK
                     GVADNAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELNLLFEK
                     QKEFGNPHISDGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPTEPKAAKNTYTAER
                     FVWLTKLNNLRILEQGSERPLTDTERTTLIDEPYRKSKLTYAQARKLLELDNTAFFKG
                     LRYGKDNADASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTD
                     EDITGRLKDRVQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYG
                     DHYGKKNTEEKIDLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETARE
                     VGKSFKDRKEIEKRQEENRKDREKAATKFREYFPNFVGEPKSKDILKLRLYEQQHGKC
                     LYSGKEINLGRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFN
                     GKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEEGFKERNLNDTRYVNRFLCQFVA
                     DHMLLTGKGKRRVFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQK
                     ITRFVRYKEMNAFDGKTIDKETGEVLHQKAHFPQPWEFFAQEVMIRVFGKPDGKPEFE
                     EADTPEKLRTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDES
                     ISVLRVPLTQLKLKDLEKMVNREREPKLYDALKARLETHKDNPAKAFAEPFYKYDKAG
                     NRTQQVKAVRVEQVQKTGVWVRNHNGIADNATMVRVDVFEKGGKYYLVPIYSWQVAKG
                     ILPDRAVVAFKDEEDWTVMDDSFEFRFVLYANDLIKVQLKKDSFLGYFSGLDRATGAI
                     SLREHDLEKSKGKDGMHRIGVKTALSFQKYQIDEMGKEIRPCRLKKRPPVR"
     gene            41868..42782
                     /gene="cas1"
                     /locus_tag="WGR04_000208"
     CDS             41868..42782
                     /gene="cas1"
                     /locus_tag="WGR04_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEZ0793848.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNDESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAEHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QFWQHIVRQKILNQAFVTDETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARALTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLVVIHLYEQGRLKTELTPSIKQHLIKTLHCQTSIERQHFSTLAAIDKMVS
                     SFQAGVTDKNAKQLKLPEILPLKAYQYE"
     gene            42775..43101
                     /gene="cas2"
                     /locus_tag="WGR04_000209"
     CDS             42775..43101
                     /gene="cas2"
                     /locus_tag="WGR04_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEZ0793849.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     repeat_region   43165..43793
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=43165..43200
                     /rpt_unit_seq="attgtagcactgcgaaatgagaatgggagctacaac"
     gene            complement(44080..44280)
                     /locus_tag="WGR04_000210"
     CDS             complement(44080..44280)
                     /locus_tag="WGR04_000210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793850.1"
                     /translation="MFGNHGLCGLLKEGAHGKSPKWLGGNLGDFGEFCKGLILQDFSL
                     IIKQGTAKIPAATGIFVRLSPV"
BASE COUNT        11361 a        12799 c        10631 g         9521 t
ORIGIN      
        1 aaaatgccgt ctgaactgtt cagacggcat ttttgatttt caaaccggaa ttacagcccc
       61 gctgccgccc tcaatgcagc agctttgtcg gtgcgctccc aagtgaactc aggttcttcg
      121 cggccgaaat gtccgtaagc ggcggattta ctgtaaatcg ggcgcaagag atcgagcatt
      181 tggacgatgc ctttggggcg caggtcgaaa tgttcgcgaa ctaaggtaat cagtttttcc
      241 tcgctgattt tgcctgtgcc gaaagtgtcg atggaaatcg aagtcggttc ggcaacgccg
      301 atggcgtagg aaacttggat ttggcattgg gttgccaatc ctgcggcgac gatgtttttc
      361 gccacatagc ggcaggcgta ggcggcggaa cggtcaacct tggacgggtc tttgccggaa
      421 aacgcgccgc cgccgtgcgg agccgcgccg ccgtaggtat cgacgatgat tttacggccg
      481 gtcaaaccgc agtcgccttg cgggccgccg atgacgaagc ggccggtcgg gttgatcagg
      541 tatttggttt cgtcggtcag cagttcagac ggcaggacgg gcttgataat ctgctcgatg
      601 acggcgtttt tcagttcttc gtaagcgatg gacggatcgt gctgggtaga gaggacgacg
      661 gtatcaatgc gttttacctt accggtttcg ctgtcgtaaa ccacggtcaa ctgcgctttt
      721 gcgtcgggac gcagccacgg caggcggccg tctttacgca attcgctttg gcgttgcatc
      781 aggcggtggc tgtaatagat ggcaaacggc atcagggtcg gggtttcgtc gcaggcataa
      841 ccgaacatca aaccttggtc gcctgcgcct tggttcaagt cgatgccttc gccttcgttt
      901 acgccttggg cgatgtcggg ggattgttgg tcgtagtaca cgccgaccgc gcagccgttg
      961 gcatcaaagc ccagctcgga ggagttgtag ccgatgcgtt tgatggtttc gcgtgcgact
     1021 ttgatgtagt ccacttgggc ggtggtggtg atttcgcctg ccaatacgca caagcctgtg
     1081 ttgaccaaag tttctgcggc gacacgcgct ttggggtctt gcgccaagat ggcatccaaa
     1141 atcgcatcgg atacttggtc ggcaactttg tccggatggc cttcggatac cgattcggaa
     1201 gtaaacagat attcgctcat tgagattttc cttgaaaaac aaaccatctt cttcagacgg
     1261 catgttgtat gaacataatg tcgacagcgg gaaatatagc aaaatttccc tattcatacc
     1321 attcagttga gaaatattcc catttgaatg gcactttgga atctctgccc gtacgtttct
     1381 tacaggcaaa aaaattcccg catcaagcgg gtttggattg ctctggtgag ccacatcggc
     1441 ttttcaaccg tccaccttac ttttcctttt gaaaagcagg ttggcatgga attcccaact
     1501 cttaatgcag cgcggatggt agcagaaaag cctttccgcc gcaatacttc cctttttcag
     1561 acggcatgtt tcgtttacaa ttcgggctgt ttcccccttt gcgaaccgcc atgcacatcc
     1621 tgttgaccgc cctgctcaaa tgcctctccc tgctgccact ttcctgtctg cacacgctgg
     1681 gaaaccggct cggacatctg gcgttttacc ttttaaagga agaccgcgcg cgcatcgtcg
     1741 ccaatatgcg tcaggcaggc atgaatcccg accccaaaac agtcaaagcc gtttttgcgg
     1801 aaacggcaaa aggcggtttg gaacttgccc ccgcgttttt cagaaaaccg gaagacatag
     1861 aaacaatgtt caaagcggta cacggctggg aacatgtgca gcaggctttg gacaaacacg
     1921 aagggctgct attcatcacg ccgcacatcg gcagctacga tttgggcgga cgctacatca
     1981 gccagcagct tccgttcccg ctgaccgcca tgtacaaacc gccgaaaatc aaagcgatag
     2041 acaaaatcat gcaggcgggc agggttcgcg gcaaaggaaa aaccgcgcct accagcatac
     2101 aaggggtcaa acaaatcatc aaagccctgc gttcgggcga agcaaccatc gtcctgcccg
     2161 accacgtccc ctcccctcaa gaaggcgggg aaggcgtatg ggtggatttc ttcggcaaac
     2221 ctgcctatac catgacgctg gcggcaaaat tggcacacgt caaaggcgtg aaaaccctgt
     2281 ttttctgctg cgaacgcctg cctggcggac aaggtttcga tttgcacatc cgccccgtcc
     2341 aaggggaatt gaacggcgac aaagcccacg atgccgccgt gttcaaccgc aatgccgaat
     2401 attggatacg ccgttttccg acgcagtatc tgtttatgta caaccgctac aaaatgccgt
     2461 aacgaaaata aaaatgccgt ctgaacaatt tcagacggca ttttgtcatt tgacgatttc
     2521 cgacagcggc cagcgcgggc gtacattaaa tgcaccggct tccctacccg ttttcaaatg
     2581 catcgcgcct gcaaaggcaa tcatggcacc gttgtccgtg cagtatgccg tcggcgggaa
     2641 aaacacgctg actttttcgg acggatgttt cggcttgcct gttggggtcg ggatttgcac
     2701 cgtcatgctg ccgaaagttt cacggagttt gcggtttgca ccgaccccgc cggcgaccac
     2761 tacggttctg aaccctgtct gcaacagggc ttttttcact ttcgccgcca acacatcgac
     2821 caccgcatct tgaaacgcac ggcagatgtc gttgcgtgtc tgctcaggaa tgtcatccgc
     2881 cccgttttcc gcgcgcactt tctcaacggc ggtcaatacg gcggttttca aacctgaaaa
     2941 actcatctgc aaatcgtcgg aatgaatcat cgggcgcgga aaaacaaacg cttcgggcct
     3001 gcccgattcc gcaagttccg acagtttcgc accgcccgga tacggcaagc ccagcagttt
     3061 cgccgttttg tcgaatgcct cgcccgccgc atcatcgacg ctctcgccca aaagcgcgta
     3121 gtcgcctatg cccctgaccg ccataatctg cgtatgcccg cccgaaacca gcagcgcgac
     3181 aaaaggaaag tcgggttttt cctccgccaa cagcggcgac agcagatgtc cttccaaatg
     3241 atggacggga ataacaggct tgtccaacgc taaagccagc gcgttggcgt agctcgaacc
     3301 cgccagcagc gcaccgccca aaccgggccc ctgcgtaaag gcaaccgcgt caatgtcgcc
     3361 atacgatgcg cccgcctgcg ccaaacagcc ttcagtcaac ggaacaaggc ggcggatatg
     3421 gtcgcggctt gccaattccg gcacaacccc gccgtattcg gcgtgcattg ccatttgagt
     3481 gtgcaggcag tgcgcccgca atccacgttc cgtatcgtaa agcgcaacgc cggtttcgtc
     3541 gcaagaagac tcgattccta ataccaacat ggtctgatgc cgttaaaaac tgaaaaacgt
     3601 attttagcgg atttcggcac gactgccgta tcccaaaaac ggaacatgcc gtctgaagac
     3661 cgttcagacg gcatcgtcgc accgtatcaa agcgttccgt aagaatgcag cccgctcaaa
     3721 aacatattca cgccgataaa ggcaaatgcg gttacgaaca aaccgataat cgcccaccac
     3781 gccagcactt tgccgcgcca accggcaacc agccgcaagt gcagccaaac ggcgtaattg
     3841 agccagacga tgaacgccca cgtctctttc ggatcccaac tccaatagcg tccccaagca
     3901 tctgccgccc acagcgcacc caaaatggtg gcaatggtaa agaacagaaa gccgacggca
     3961 atcgccttat acatcacctc gtcgatcaat gccgacggcg gcagccacag ttttccgcct
     4021 tttccttccg cacgcaggga aaccagttcg gcaataccga gcatcgcgga aatgcaaaac
     4081 gcgccgtaac cgataaagtt tgccggaacg tggattttca tccaccaaga ttgcaatgcc
     4141 ggaatcagcg gctggatggt atgcgcctcg cgggacacgc tgtaccacaa gacaaatcca
     4201 accacgaccg ccataaagcc gaacacgaag ccgcccaatt tctgtatggc gaacttacct
     4261 tcataataaa gatacatcag cgcggtaatc accaaaaaca ggatgaacac ttcatacagg
     4321 ttggaaaccg gaatatgccc cgcatcggga cggagcagat agctttcgtg ccaacgtacc
     4381 agcagaccgg taaagcctgc tacggcagac acccatgcaa acacggttcc catacccaac
     4441 agcgtgttgg tcggcacatt ttttacgctt gccaaaaccg cgcccgaaat ataggcgaac
     4501 agggcgaaaa agacaaaggc gcactgccac ataatcgccg actggctgct gaggaaatac
     4561 cgcaacagga aaatctctgc cgatttgatg ttgccgccat acagaccgac ggcggcatag
     4621 gcaagcaata cgcttaaagg aacaaaccag cgcatcggtt tgaaaaacca acccaaaaac
     4681 acggcaatac cggcactcgc ccacaacatg accgtttcgt aaatgtccat atgcataccg
     4741 gaacgggtct gtacgaaaac cgtagccgca aaaaccagca cggcaaatac ccaatcccaa
     4801 agattcagat tgctgatcaa agacttctga atcagcagct cgtgttccgg aagggtttta
     4861 tagtgttcag tcatgattca agtccttgcc gagccgttgc agactctcga cgtgttttgg
     4921 aaattccttc tgcaaatccc gttcgctgcg ggccgaagac atggcaaaac ggattttgcc
     4981 gtctgaaaac aatacccacg cccgtttttc gcgcacataa aacatcaata ccgtacccaa
     5041 taccaacagc accgagccga gatagaccaa aagcgcaccc ggggaacggg tcatctgcaa
     5101 acccgacgaa cgcacctcgg aaaacccatc aagttgcagc agcataggcg cgggatattc
     5161 ggtcaaaccc gtgtacgcat ccatactgtg cagcaggaaa cggttccgcg cttcatcctg
     5221 ctgccattcg ggcaagccgt accggcgtat ggtttcatcc aaagcagcgt tcatcacgcc
     5281 gtaaagcatt tcgtagaaat aaccctgcat cttatcctgc tgctctttcg ggatattgga
     5341 cgtaataaat tcgtccaatc ccaaatagcc tttttgtgca aagatgttca gcgtgttttc
     5401 cgcagccagc atgaattgtt cgcggatttc ggcaggtgcg tctttggttg cgtcggcaac
     5461 cagacgtttg cgcccttccc catctttcaa aaactcacgc aatgccataa aggtgtccgc
     5521 tttcaactgc ttgtccaagg ggatacgcag ccagcggtat tgctgctgca agccgctgcg
     5581 cgtgccggta atccaaaaat aatcctgttc ctgcaaaacc ggcagcatat agtttttata
     5641 ttcgaccgcc tgccctgccg catcacggat acggtaaaca atggaagggc cgatattggt
     5701 gtatttttta ccttcctgag taacggcgcg gacatcgttc agcgtggatt tcaggctttt
     5761 ttcccgttcc gcgccctcgc tcatgtcctc cacattcata gaagtgaact gatcgaactc
     5821 aagacgatat ttgtgtttgc caatttccaa cggaaactgg tgtatggatg ttgccttcaa
     5881 cacgacaggc tcgcgcgaag catcacccaa attccacgcc ttgaatgtca aatccgaacc
     5941 gccgtcggca aaactcgcct gataaatcgt gatgccgtgc aaggtcaaag gatggttcac
     6001 gcggatggtg cgctcgagtt tctcaccggt tgccttatcc gttacttcaa tatcactggc
     6061 aaaatcgcgc ggcatacccg tattgtaaaa atcgatatgg aattttttca gtttgacttc
     6121 aaaaggcaag tcctgaacca atatcccgtt gtcggcattc aggaaaacca catccgcact
     6181 ctgcccctcg gaaatattga cgttgcccct aaatgagaga ttggacgcac ccaaaatact
     6241 ttcgggcttg aaatccttgg cataaaccgc ctgattgtcc ggaacaatcc gaccggtcag
     6301 catacccagt ttcaacagca ggttactgtc tatcaacccg cccaggcaaa tgacaatcaa
     6361 agcaacatgg gcaaagatat agccccattt gttcattgtg ccttttttgg cggcaatcag
     6421 aaccgacccg tcttcacggt taatggtttt tccctgaaaa ccttgtactt ccagataacg
     6481 tttggcaacc tcgggcgcaa tttttacatc caacagcgaa gaatggcgca tcgccgccag
     6541 agatttttct ttaacctttt cccgaaaaga cttcatttcg cgccagaacg gcggcacatt
     6601 gcgaatcagg cacaaactgg tagaaaccac caaaaacatc atgataacga caaaccatgc
     6661 cgaagcatag acgtcataca gtcccagaaa accaaaaatc tgcgcccaaa acgatccgaa
     6721 tttgaccaaa taatccgtct gcggctggtt ttgctgcaac accgtaccga taaccgatgc
     6781 aatacccagc agactgagca aagcgactgc aaagcgcatg gagctgaaaa aagcgaacca
     6841 cggacgggaa agaagtgggg gagatctacg ggatttactc attgtgtgtt tattccgcca
     6901 tcaggaatat gggaaagcag aattgggcaa acagaaaaca acgtcccgat tctactgtct
     6961 tgatgctttt tatttcaaga caatgaagac agcctgcatc gattccaacg gttgcgattg
     7021 aaaaaactta tcgcagaatt gcctgaagcc gtctgaaaac ttttcagacg gcctctaaaa
     7081 cagactattg cggaattaac gcaaaccttg gataaagttg gcgaccgctt tcaaatcttc
     7141 ttcagacata cggtttgcaa tatcttccat gatggtattt ttacgctgac cggacttgta
     7201 ggcattcatc tgttcaacaa tatatgcctg atgctgaccg cccaaacgcg gataagcctg
     7261 aatttcgctt ccgcctcccg gcatacccgc accgctcgga ccgtggcagg acatacacgc
     7321 cggcactttt ttatcgctca aaccgccgcg atagattttc gcacccaatt cgggattttc
     7381 cttaggattg gcttcaccgg atttgggctg ctgtttggca tagaatgcgg atacgttcaa
     7441 aatatcctga tcgctcaaat tcattaccac cggtttcatc acagctgccg aaccgtgggt
     7501 gcgtttaccg tcgcggatgc cgatagtttg atgatagatg taagcagtat gctgtgccgc
     7561 caaacgcgga tacatcgcaa tgccgctgtt accgtctgct gcatggcaag ccgcacaaac
     7621 cgttgcggca acctgtttgc ctttttccac gtctgctttg ggagaggcgg aaaccgcacc
     7681 ggcagccaaa acaaaggcca ataaagtcaa tcgtttcatg gagtgctcct gattacagca
     7741 ttggataacg caacaatgct ctttttatat tcaaatacgg gatttttgac ccgattaaaa
     7801 ccgatgattc tgtaaacgtg ttattctata ctaaatttac attaaattac cactgtgttt
     7861 cacataaaac caaccgcata tttttgctgt cggacaaacg gcggcggaaa acaaggatat
     7921 gcccatgaac ctttttcaaa acgccaaatt cttcacgacg atcaaccacc ttaaagacct
     7981 gcccgacacc cctctcgaaa ttgcctttgt cggcaggagc aatgccggaa aatccagtgc
     8041 catcaatacc ctgaccaacc atgtccgtct tgcctacgtt tcaaaaacac ccggacggac
     8101 gcagcatatc aacttcttcg agctgcagaa cggcaatttt atggtcgatt tgcccggcta
     8161 cggttatgcc caagtccccg aagcagtacg cgcacattgg gtcaatctgc tcggcgacta
     8221 tctgcaacag cgcaaacagc ttatcgggct ggttttgatt atggatgccc gccatccttt
     8281 aaaagaactc gacatccgta tgctggattt tttccacacg accggcagac cggttcacat
     8341 cctgctgtca aaagccgaca aattatccaa aaacgaacag ataaaaaccc tgtcccaagt
     8401 caaaaaactg ctcaaacctt attccgacag gcaaaacatc agcgtacagc tgttttccag
     8461 cctgaaaaaa caaggtattg acgaggccaa ccgaactgtc ggaagctggt tggacgcagc
     8521 agatgccgcc gcttcctctc cagaggaaaa ctgaccccaa ttatacggaa accgtattcc
     8581 ccccacttga ccgaccgcaa acatttaaaa aattgccact gccaaatcta aaatgccgtc
     8641 tgaaaagtct ttcagacggc attttgcgga gtctttaaaa cagagaatcc aactgctgct
     8701 gtttggaacc agtattactc ggaagcaccg gcgtttcctg catatcttgg cggacttcgt
     8761 catccgccgc ctgccgtccg ccttctgccg cgcccccgtc atcttctttt gcccgtcggg
     8821 aaggttgcgg cgcaataccg ctgttgtcca gcgtcaagcc cggatcggtt accatacgtt
     8881 ccttcatata gtattcgcca ttgctgctga ccacaccttc aggcattttc atccccttgc
     8941 cctgctttcc tttcaacgca aaacgcatat agtccaccca aaccggcacc gcaatcgtac
     9001 cgccgtagcc gacacgcccc atactcttag gtttgtcgaa gccgatatat acggcagtaa
     9061 ccacatcagg gttaaaaccg acaaaccacg catccttatt gtcattggtc gtacccgttt
     9121 taccggcaat atccgttctt cccaacgcag ctgcccccct tgccgtacca acacggacca
     9181 catcctgcat aatcttatac ataatatagg cattgcgcgg atcgattgcc tgaggcgcat
     9241 tttgcccagc caccaaaggt tgcatttggg cgcgcaacct gccgtctctg tcataaatct
     9301 tatcgattac gtgcgaagaa accctatatc cgccgttcgc aaatacgcta tatgcctccg
     9361 ccactttcaa cggcgttgtc tcgcccgtac ctaaagccat agacaggctt gccggcagct
     9421 cggacgacct gaagccgaaa cgccggatat actgttgcgc gtaaccgaca ccgatagaca
     9481 tcaaaatacg gatggaaacc atattcttgg aagccgtcag agcctgtctc aaagtaatgt
     9541 agccggaata tctgccgtct gaatttttag gtgtccaaac cgaaccgttc ggccctttcc
     9601 ccggcaggga aatcggcgca tcgttaacca ctgtggacgc ggtcatcccc ttagataatg
     9661 ccgccgaata gacaaacggc ttaaaggtcg aacccggctg ccgcattgcc tgaacggcac
     9721 gattgaatgt tttgctgtga aaatcataac cgccgaccag cgcgcgcaca gctccggttt
     9781 ttgcatccag cgaaaccaaa gccccctgca gcaacggctc ttgaaccacc gcccaacgcc
     9841 cgccgttgtt tttgacacgg atgaccgcgc ccctgcggat acggtcctcc cccatttttt
     9901 cattattgac cgcgcgggcc gcaaaaccca aggcgcgcct gtcaagcgta acccgcctgc
     9961 cgccgggcag ctgtatgacg acatttttct ttttagtcac atccaatacg acagcgggaa
    10021 ccattttatc gacggtatag agtcccgaca gatactggct gacagtctcc tcgacatctt
    10081 cactcttact caaatcgata tagttttccg caccgcggta gctgctgccg cgatcgaaat
    10141 tccgtagagc cttgcgcaat gcctcggttg ccaccttctg atgatcggcg cggaccgtgg
    10201 tataaacctt aaaaccctgc gtataggcat cttcaccgta tttctcatac agttcctgac
    10261 gcaccatttc cgccacatat aaagcactct gatcgatttt ccgaacaaac cgctcgtaat
    10321 gcagttcctc attcaacgcc tgatcgcgct gttgcacggt aatcatcttc tcctcgagca
    10381 tattgttcaa aatatacttc tggcgcaact tggcacgttc cggattaaca atcggattat
    10441 aggcagacgg agccttgggc agtcccgcaa gcatggcggc ttccgccaaa gtcaaatctc
    10501 ggacattctt attgaaatag atttgcgcgg cagatgcaaa accataggcg cgctgaccga
    10561 ggtaaatctg attgaaatac aactcgagga ttttgtcttt gcttaaagac tgctcgattt
    10621 tataggcaag caacacctca ttgaatttgc gtgtgaacgt tttttcactg ctcaaataaa
    10681 aatttttcgc cacctgctgc gtaatcgtac tcgcacccga ctgcacgctg ccggacacga
    10741 cattgccgac ggcagcgcgg gcaacacccc aaacatccac cccccaatgc cggtaaaagc
    10801 gtttgtcctc ggcggcgata accgcattcc gcaacacctc tgggaaatcg ccgatttttg
    10861 taaattcgcg ccgctgctcc ccatacatac cgatgacttc cccatccgcc gaataaatag
    10921 tcaacggcat tttaggctgg taatgctgca aagaatccaa agacggcagt ttcggatacg
    10981 ttaccaaaat agcaatggca accaaaccca ctccaaatac acaaaaccca aaaaccaaac
    11041 caaaacaagt cgttaaaatc tttttaatca tagctgaata ataatttacc attattggta
    11101 ttaaataaag taaaatagca accgatttct acaaagcacg gtttcaatgt gcaaagaaca
    11161 aggaatccat tacggatacc gaaacggtta ctcactgtac aaataaagca ggaactttca
    11221 tcatgcgctt gtttaaaagc ttgaaaaacc ctaaaaaaac agatgccaag ctccctaaaa
    11281 aatcttcggg actcaataac cgcgcggcaa tcggcatcga tatcgaccag cattccatca
    11341 aaatggtcca attgtcagga cgtagtttaa accaaattca attggaaaaa tacgtcattg
    11401 ccaaattacc aaagaatatc attcaaggca ataaagtcca aaattacgat caacttgtta
    11461 catatttgca acaagcctat gccaaactgg gtacttcgtg caaaaacatc atcgcgtccg
    11521 tcccgcaaaa tctggcaacc atcgaacaat tgacctacac agacaaagat gcagaattag
    11581 acctgcaggg gttcgtggag tcctccatct ccgaagtcag ctcgatatcg ctcgaagaag
    11641 ccaattacga ctatcaggtc ttgtcccaat cggccgccgg cgaagctgtg ttggccgtcg
    11701 catcgagaaa ggatgaaatc gaacccctga ttgacgcatt caacgccgcc ggtatgaaat
    11761 tatccgcgct tgatgtggac attttcggac aatacaacgc ctacgcgcta tggataaacc
    11821 atttcgcccc cgagcttgca gccgaaaaag tcgccatttt cggcgtatat gccgcacaga
    11881 cctacgcctt ggtcatccaa gacggaaaaa tcctatacaa acaggaaacc tccgtcagcg
    11941 aagaacagct caaccaactc atccagcgca cctatcaggt aacagaagaa aaagcggaag
    12001 aaatcatcaa ctccccgcaa aaaccttccg attaccaaga aagcgtggca aactatttca
    12061 accagcagat tacccaagaa atacaaaggg tcttgcagtt ttattacacc acgcagaccg
    12121 cagacgatat gaccgacatc aagcatatcc tgctgaccgg ggaagcggcg cgccaggaag
    12181 gcatcgccca aaccgtcgcc tcacaaacca atgcagatgt acaatgcgtc catcccgcgc
    12241 gttattttgc ggacaacctc aaaacagaca aacaacaatt cgaacttgat gcgccgacac
    12301 tgaccagggc gttcggtttg gcggtacggg gattataatt atgaacaatt taatcaaaat
    12361 caacctcctc ccctacaggg aagagatgaa caagcgcaaa cagcagcagt ttaaaacgct
    12421 gatgtacggt gccgtgctga cgggcgttgc cgccgttgcg gcaacctacc tgtttatcga
    12481 caatatgatc aataaccagt cggaaagaaa cacgctgctg gaaacctcca tcgcacactt
    12541 ggataccgag ctgtcggaaa tacaaaagct caaacaggaa aaagatgcct tcctgattaa
    12601 gaaaaacaaa atcgaggagc tccagctcaa acgcctccaa gccgcaaaaa tcctcgacag
    12661 cctgaatgag gccgtccccg gaagcaccta cctgacctcg ctggatgccg ttaccgccga
    12721 ctcttatcgg ctcagcggca ggacatccag cgacaaccgc gttgccgcca tgatgagggc
    12781 gatgcccaat accggcatat tcaagcaacc cgaattgtta agcatcaaga aaaacaattc
    12841 gcatcaagaa tttacccttc aggcaacatt acaacccatc gtaaaggcgg ccgaatccaa
    12901 agagaatccg gcttcgggaa acgcacagga ggcaaactga atggcttcta aatcatctaa
    12961 aaccaacttg gatctcaaca accttcacct gctcaacctt cctgccaggc tttttatcgc
    13021 cctgctggcc gttgccgccg tgctggggct cggttatgcc ggattgttca aaagccagat
    13081 ggaatccctt gaggaatacg aagcaaaaga aaccgaactg aaaaacacct acaaacagaa
    13141 aagtatcgac gcggccagcc tgaacaacct gagggacgaa cttgcctcaa tccgctctgc
    13201 cttcgatatc atgttgaaac agctgccgac agatgcagaa attcccaatc tggttcaaga
    13261 gcttcatcag gcaggttcga gcaacggtct gcgcttggac agcgttatgc cccaacctcc
    13321 cgtagatgac ggccccatca aaagattacc ctattccatt tccattaccg gaaattacga
    13381 acagatcagc caatttaccc gcgatgtcgg cagcctctcc cgaatcatta cccttgagtc
    13441 gctgaaaatc gcccaatctc cggaaaacgg cggcaatcct gacggcaaga gcagcatcct
    13501 gaacctcagc gccattgcca ccacctacca agcaaaatcc gtagaagagc ttgccgcaga
    13561 agcggcacaa aatgccgagc aaaaataact tacgttaggg aaaccatgaa acactatgcc
    13621 ttactcatca gctttctggc tctctccgcg tgttcccaag gttctgagga cctaaacgaa
    13681 tggatggcac aaacgcgacg cgaagccaaa gcagaaatca tacctttcca agcacctacc
    13741 ctgccggttg cgccggtata cagcccgccg cagcttacag ggccgaacgc attcgacttc
    13801 cgccgcatgg aaaccgacaa aaaaggggaa aatgcccccg acaccaagcg tattaaagaa
    13861 acgctggaaa aattcagttt ggaaaatatg cgttatgtcg gcattttgaa gtccggacag
    13921 aaagtctccg gcttcatcga ggctgaaggt tatgtctaca ctgtcggtgt cggcaactat
    13981 ttgggacaaa actacggtag aatcgaaagc attaccgacg acagcatcgt cctgaacgag
    14041 ctgatagaag acagcacggg caactgggtt tcccgtaaag cagaactgct gttgaattct
    14101 tccgacaaaa acaccgaaca agcggcagca cctgccgcag aacaaaatta agaagaggat
    14161 tactccatta tgaataccaa actgacaaaa atcatttccg gtctctttgt cgcaaccgcc
    14221 gcctttcaga cagcatcggc aggaaacatt acagacatca aagtttcctc cctgcccaac
    14281 aaacagaaaa tcgtcaaagt cagctttgac aaagagattg tcaacccgac cggcttcgta
    14341 acctcctcac cggcccgcat cgccttggac tttgaacaaa ccggcatttc catggatcaa
    14401 caggtactcg aatatgccga tcctctgttg agcaaaatca gtgccgcaca aaacagcagc
    14461 cgtgcgcgtc tggttctgaa tctgaacaaa ccgggccaat acaataccga agtacgcggg
    14521 aacaaagttt ggatattcat taacgaatcg gacgataccg tgtccgcccc cgcacgcccc
    14581 gccgtaaaag ccgcgcctgc cgcaccggca aaacaacagg ctgccgcacc gtctaccaag
    14641 tccgcagtat ccgtatccga accctttacc ccggcaaaac aacaggctgc cgcaccgttt
    14701 accgagtccg tagtatccgt atccgcaccg ttcagcccgg caaaacaaca ggcggcggca
    14761 tcagcaaaac aacaggcggc agcaccagca aaacaacagg cggcagcacc agcaaaacaa
    14821 accaatatcg atttccgcaa agacggcaaa aatgccggca ttatcgaatt ggctgcattg
    14881 ggctttgccg ggcagcccga catcagccaa cagcacgacc acatcatcgt tacgctgaaa
    14941 aaccataccc tgccgaccac gctccaacgc agtttggatg tggcagactt taaaacaccg
    15001 gttcaaaagg ttacgctgaa acgcctcaat aacgacaccc agctgattat cacaacagcc
    15061 ggcaactggg aactcgtcaa caaatccgcc gcgcccggat actttacctt ccaagtcctg
    15121 ccgaaaaaac aaaacctcga gtcaggcggc gtgaacaatg cgcccaaaac cttcacaggc
    15181 cggaaaatct cccttgactt ccaagatgtc gaaatccgca ccatcctgca gattttggca
    15241 aaagaatccg gaatgaacat tgttgccagc gactccgtca acggcaaaat gaccctctcc
    15301 ctcaaagacg taccttggga tcaggctttg gatttggtta tgcaggcacg caacctcgat
    15361 atgcgccaac aagggaacat cgtcaacatc gcaccccgcg acgagctgct tgccaaagac
    15421 aaagccttct tacaggcgga aaaagacatt gccgatctgg gcgcgctgta ttcacaaaac
    15481 ttccaattga aatacaaaaa tgtggaagaa ttccgcagca tcctgcgttt ggacaatgcc
    15541 gacacaaccg gaaaccgcaa tacgcttgtc agcggcaggg gcagcgtgct gatcgatccc
    15601 gccaccaata ccctgattgt taccgatacc cgcagcgtca tcgaaaaatt ccgcaaactg
    15661 attgacgaat tggacgtacc cgcgcaacaa gtgatgattg aggcgcgtat cgtcgaagcg
    15721 gcagacggct tctcgcgcga tttgggcgtt aaattcggcg cgacaggcaa gaaaaagctg
    15781 aaaaatgata caagcgcatt cggctggggg gtaaactccg gcttcggcgg cgacgataaa
    15841 tggggggccg aaaccaaaat caacctgccg attaccgctg ccgcaaacag catttcgctg
    15901 gtgcgcgcga tttcctccgg tgccttgaat ttggaattgt ccgcatccga atcgctttca
    15961 aaaaccaaaa cgcttgccaa tccgcgcgtg ctgacccaaa accgcaaaga ggccaaaatc
    16021 gaatctggtt acgaaattcc tttcaccgta acctcaatcg cgaacggcgg cagcagcacg
    16081 aacacggaac tcaaaaaagc cgtcttgggg ctgaccgtta cgccgaacat cacgcccgac
    16141 ggccaaatca ttatgaccgt caaaatcaac aaggactcgc ctgcgcaatg tgcctccggt
    16201 aatcagacga ttctgtgtat ttcgaccaaa aacctgaata cgcaggctat ggttgaaaac
    16261 ggcggcacat tgattgtcgg cggtatttat gaagaagaca acggtaatac gctgaccaaa
    16321 gtccccctgt tgggcgacat ccccgttatc ggcaacctct ttaaaacacg cgggaaaaaa
    16381 accgaccgcc gcgaactgct gattttcatt accccgagga ttatgggtac ggccggcaac
    16441 agcctgcgct attgatgcgt caaaataagg gcatatgttt tacggcatat gccctttctt
    16501 tatgcttttt gccgcgaccg aaatgccgtc attcccgcgc aggcgggaat ccaacttgtc
    16561 cggtttcggt tgtttttcgt ctcgtaactt ttgagccgtc attcccgcga aagcggaaat
    16621 ccaatccgtt cagtttcggt catttccgat aaattcctgt tgcttttcat ttctagattc
    16681 ccacttttgt gggaatgacg gcggaaggga taaatcctcg caatccaaag cctcgtcatt
    16741 tccacaaaaa acagcaaccc aaaacggcaa cttaaaaccc cgtcattccc gcgcaggcgg
    16801 gaatctaggt ctgtcggttc aggaccttat cggataaaac ggtttctcca accctgcgtt
    16861 ctagattccc actttcgtgg gaatgacggg atatgggttt ccgtgcggac gtgttcggat
    16921 ttccgcctgc gcgggaatga cggcgacaga tgcccaacgg tctttatagt ggattaacaa
    16981 aaatcaggac aaggcgacga agccgcagac agtacaaata gtacggaacc gattcacttg
    17041 gtgtttcagc accttagaga atcgttctct ttgagctaag gcgaggcaac gctgtactgg
    17101 tttttgttaa tccactatag tagtgataaa catattatct tcaatatatt caattggata
    17161 attgtttacc taagcaaaga taattgcctt ttcctgacaa ataagtgaaa tcaacggatt
    17221 gtcaaaacac agcctgaaat aaaaaacctc cctgatttct tttatttttc cttaaaatca
    17281 gaaaggttcg ggatggtcgg gttatttttc caaacgtacc gctgccctgc cgatttcgta
    17341 taaaattccg ccgtaacccg acaagcccga accctgtcgc cccgaaaggc ggggtgtcaa
    17401 acattaagga attgtgatga aaaactttaa cggcaaactc atcctcatcg gactgatggg
    17461 cgcgggcaaa accacgctgg gccggcaaat ggcgcagcgg ctggattacc gtttttacga
    17521 cagcgatcac gaaatcgccg cagccgccgg cgttcccatc cccaccatat ttgaaatgga
    17581 aggcgaacag ggattccgtt cgcgcgaaac cgccatactc aaaaagctgg ttatcctgcc
    17641 ccatatcgtc ctgtccaccg gcggcggcgc ggtgttaaaa gaagaaaacc gcgcccttat
    17701 ccgcaaaagc ggcacggtcg tctatctgca cgccccgccc gaaaccctgc tcgaacgcac
    17761 gcgctgcgac aacagccgtc ctttgctgca agttgccgat cctttggcga aattacgtga
    17821 actctacgcc gcacgcgacc ccgtttaccg ccaaaccgcc gactttaccg tagaatcggc
    17881 aaactgccgg gaaaccgtgc aaaccctgct caaacgctta tcccgataaa ccggcatatg
    17941 cgccgcgccc agaaaaccaa gccgcgcccc gcccggcggg ccggcggttc aaactttaag
    18001 gaacaacaat gaaaacactg accgtacaca ccccgtccca cagctacccc atctttatcg
    18061 gcaacggact gctgccgcag gcaggaagcc tgctcaaacc gcatttgggc aaacgcgccg
    18121 ccatcatcgc caacgaaacc gtcgccccgc tctacctcgg cacgcttcag acggcattgg
    18181 atgcggcagg cgtatcccat ttcagcatca tcctgcccga cggcgaggcg cacaaaaact
    18241 ggcagacgct caacctcatc tttgacgggc tgatgcaaaa ccgcgccgaa cgcaaaacca
    18301 cattaatcgc actgggcggc ggcgtgatcg gcgacatggt cggctttgcc gctgccacct
    18361 accagcgcgg cgcaccgttc gttcaaatac cgaccacact gttgagtcaa gtcgactcat
    18421 cggtgggcgg caaaaccgcc atcaaccacc cgctcggcaa aaatatgatc ggcgcgttct
    18481 accagccgca ggcggtgctt gccgacttgg acacgctgca caccctgccc gtccgcgaac
    18541 tgtccgccgg tatggcggaa gtcatcaaat acggcgcgct cggcgacatc ggttttttcg
    18601 aatggctcga acaaaacata tccggactga tgacgctcga tcgggaaaaa ctcgcccaag
    18661 ccgtgtaccg ctgctgccaa atgaaggcag acatcgtcgc ccaagacgaa accgaacagg
    18721 gcatacgcgc atggctcaac ctcggacaca ccttcggaca cgccattgaa accgagatgg
    18781 gttacggcac ttggctgcat ggagaagcca tcgccgccgg ctgcgtgttg gcggcgcgtt
    18841 tgtccgaaca actgggcaaa acctccgccg cagataccgc gcggctcgcc gccctgctcg
    18901 aaaccgccaa actgccgtcc gcaccgcccg tgttcccatt tgaaaaatgg ctcgaacata
    18961 tgagccacga caaaaaagtc agcggcggcg tgatgcgttt tatcgggttg aaccggctgg
    19021 gcgaagccgt cattaccgaa atcaccgata cggacatcct ccggcgcacc ctgcaaccgt
    19081 atctctgatt tcctctgccg atgtgctgcc gcgcgggttt gacgcacgat gatgattttc
    19141 catcatcttt ctccgtaaaa gcgggaatcc ggtccgttcg gtttcggtcg tttccgataa
    19201 attcccgttg cttttcattt ctagattccc actttcgtgg gaatgacggc ggagaggttt
    19261 ttgttgtttc ggagaagttt ctgcaaccct agaatctcgt tatttccaca aaaaacagaa
    19321 acccaaaaca gcaacttaaa acctcgtcat tcccgcaaaa gcgggaatcc ggtccgttcg
    19381 gtttcggtcg tttccgataa attcctgctg cttttcattt ctagattccc actttcgtgg
    19441 gaatgacggc ggaagggttt tggttttttc cgataaattc ttgaggcatt gaaattctat
    19501 agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacag atggtacgga
    19561 accgattcac tcgtgcttca gcaccttaga gaatcgttct ctttgagcta aggcgagaca
    19621 acgccgtacc ggtttttgtt aatccactat aacagcaacc ctgtcgccgt cattcccgca
    19681 aaagcgggaa tccagtccgt tcggtttcgg tcgtttccga taagttcccg ttgcttttca
    19741 tttctagatt cccactttcg tgggaatgac ggcggaaggg ttttggtttt ttccgataaa
    19801 ttcttgaggc attgaaattc cagattcccg cctgcgcggg aatgacggct caaaagttac
    19861 ggaacgaaaa acaaccaaaa ccggacaagt cggattcccg cctgcgcggg aatgacggaa
    19921 tcttaagttt ccgtctttgt tttctgtttt ctgttttcga gggaataatg gggaacaagc
    19981 cgtatttcag acggcatttt cagttcgggg tataatccga atacttgcga ccatctgaat
    20041 catttggaca aactatgtgt caactgctgg gcatgaactg caatacgccg accgatatta
    20101 tgttttcctt tgaaggcttc cgccggcgcg gcggcattac cgaccatcat gccgacggtt
    20161 tcggcatcgg ctttttcgaa ggcaaaggcg tgcgcctgtt ccatgacgac aaacccagtg
    20221 ccaattcgcc cgtcgccgac ctcgtgcgcg cctaccagat taaatcggaa aacgtcgtcg
    20281 cccatatccg caaagcatcg caaggacaaa cctcgctggc aaacacccat ccctttatgc
    20341 gcgaaatgtg gggcggctac tggctgtttg cccacaacgg gcatttggtc gacttctttc
    20401 ccgaacaggg cgaatttttc caacccgtcg gcacaaccga ttccgaacgc gcgttctgcc
    20461 acatcctcaa ccgcctgcgc acacgctttg tcgcccgtcc cgacgacgac acgctgtttg
    20521 gcgcgattgc ggggctgacg cacgaaatcc gcaaattcgg gctgtttaac tttatgcttt
    20581 cagacggcat tgccctgttt gcccacgcca gcacactgct gcactacatc gtccgccaag
    20641 ccccgttcgg caaggcgcgc ctgctcgacg acgacgtaat ggtcgatttt gccgaagtaa
    20701 ccacgccctc cgaccgcgtc gccgttatcg ccaccctgcc gctgacgcgc gatgaaacgt
    20761 gggcgcaact tgccgttgac gaactggtga tgttccgcga aggcaaaatt attttgaacg
    20821 accgaccccg gcaccccgtt tatatgagtg tcgaagaagg tttggaaacc gcgcgcgccg
    20881 ccggcgtcgc cgtctgaact tcagacgaca taggaggacg aacccgatga aatgcccgtt
    20941 ttgcgcccac cccgacaccc gcgttgccga ttcgcgcctg atggacgaac gcaacgccgt
    21001 ccgccgccgc cgatactgcc ccaactgcgg caaacgcttc ggcacactcg aaaccgccga
    21061 actcaaaatg cccgccgtca tcggcacgga caaaaaacgt tcgcccttca atgcacaacg
    21121 cctccgcaac gacctgaccg ccgccgcccg aaaatccgcc ctgacacccg aacagatcga
    21181 cgaaaccgcc cgcctcacgg aactcaggct gtatgcttcg ggtttgcgcg acatatccgc
    21241 tgccgaactt gtcgaaatcg tactggaaga gctgttcggg caaagcacgg aagccgccgt
    21301 ccgtttcgcc gccctgcaca aacgcttcga caatccggca gactttgcct cgtggctggc
    21361 gcaagccgtc aaaacaggcg gcaaagcctg attctcccca acccatactg atacggtatc
    21421 cccatgtttt cggacacaga tatatccatg atggaaaacg ccctccgact tgccgctttg
    21481 gggcgttttt ccacttcgcc caatccgcgc gtcggctgcg ttatcgcaca cggcagccaa
    21541 attgtcgggc agggcttcca cgtcaaagcg ggcgaacccc atgccgaagt ccacgccctg
    21601 catcaggcgg gcgaaatggc acaaggctcg accgcctttg ttaccctcga accgtgcagc
    21661 cattacgggc gtaccccgcc ctgtgccgaa gctctgctcc gttccggcgt aacacgcgtc
    21721 gttgccgcca tgcgcgaccc caacccgctg gttgccggca aagggcttgc cctgctcgaa
    21781 gcagcaggca tcaagacgga atgcggttta ctcgaacatc aggcaaggga actcaaccga
    21841 ggcttcctgt cgcgcatcga acgccgccgc cccttcgtcc gcctcaaatg cgccgtttcg
    21901 ctggacggca aaaccgccct ttcagacggc agcagctttt ggattaccgg cgaagaagcg
    21961 cgtgccgacg tacaaacttt acgcgccgaa agctgcgcgg tgctgaccgg catcggcacg
    22021 gtgttggcgg acaatccccg gctcaacgtc cgcgcttttc caactttgcg ccaacccgcg
    22081 cgcatcgtgt tagacagccg cctgcgcctg cccttggaca gccgccttct gaccgacgga
    22141 caatctccaa cctacatcgc caccttggaa cgcgatgaag acaaactccg cccctatcgc
    22201 aaacacgccc acgtccgcat cctgatgccg tctgaaacgg caggcggcaa aatcgatttg
    22261 aacagcctga tgcgcctgct tgccgacgaa ggcatagggg aactcatagt cgaagcaggc
    22321 tccgaactca catctgcatt tttggcagaa gatttggcag acgaaatcgt gctgtaccgt
    22381 tcgcccaaaa tcctcggcag cggcaaagac ctgttttccc tgcccgaaaa ccgcgccgcc
    22441 ctttccgcac cgcccttgtg gacacccgtt tcaagcgaaa tcctcggaca cgacatcaaa
    22501 accgtgttcc gaaaaaacgg caacgccttt taaagggttt gcgccgtttc actatataat
    22561 aacgccgata aaaaacggcc ccgttcagac cgccgcccgc ccggaaaagc gcaacccgga
    22621 ctgccgcacc ccgccggcag ccgcaacgga ctgaaagccg tcaaattccg atcaagaaag
    22681 gcttcagacg gcacaggcag catcccgccg ccgcccggac atcaaaaatg gacacaaaag
    22741 aaatcctcgg ctacgcggca ggctcgatcg gcagcgcggt tttagccgtc atcatcctgc
    22801 cgctgctgtc gtggtatttc cccgccgacg acatcgggcg catcgtgctg atgcagacgg
    22861 cggcgggact gacggtgtcg gtattgtgcc tcgggctgga tcaggcatac gtccgcgaat
    22921 actatgccgc cgccgacaaa gacaccttgt tcaaaaccct gttcctgccg ccgctgctgt
    22981 ctgccgccgc gatagccgcc ctgctgcttt cccgcccgtc cctgccgtct gaaatcctgt
    23041 tttcactcga cgatgccgcc gccggcatcg ggctggtgct gtttgaactg agcttcctgc
    23101 ccatccgctt tctcttactg gttttgcgta tggaaggacg cgcccttgcc ttttcgtccg
    23161 cgcaactcgt gcccaagctc gccatcctgc tgctgctgcc gctgacggtc gggctgctgc
    23221 actttccagc gaacaccgcc gtcctgaccg ccgtttacgc gctggcaaac cttgccgccg
    23281 ccgccttttt gctgtttcaa aaccgatgcc gtctgaaggc cgtccggcgc gcaccgtttt
    23341 catccgccgt cctgcatcgc ggcctgcgct acggcatacc gatcgcactg agcagcatcg
    23401 cctattgggg gctggcatcc gccgaccgtt tgttcctgaa aaaatatgcc ggcctagaac
    23461 agctcggcgt ttattcgatg ggtatttcgt tcggcggagc ggcattattg ttccaaagca
    23521 tcttttcaac ggtctggaca ccgtatattt tccgcgcaat cgaagcaaac gccccgcccg
    23581 cccgcctctc ggcaacggca gaatccgccg ccgccctgct tgcctccgcc ctctgcctga
    23641 ccggcatttt ctcgcccctc gcctccctcc tgctgccgga aaactacgcc gccgtccggt
    23701 ttatcgtcgt atcgtgtatg ctgccgccgc tgttttgcac gctggtagaa atcagcggca
    23761 tcggtttgaa cgtcgtccga aaaacacgcc cgatcgcgct cgccaccttg ggcgcgctgg
    23821 cggcaaacct gctgctgctg gggcttgccg taccgtccgg cggcgcgcgc ggcgcggcgg
    23881 ttgcctgtgc cgcctcattt tggctgtttt ttgttttcaa gaccgaaagc tcctgccgcc
    23941 tgtggcagcc gctcaaacgc ctgccgcttt atatgcacac attgttctgc ctggcctcct
    24001 cggcggccta cacctgcttc ggcacgccgg caaactatcc cctgtttgcc ggcgtatggg
    24061 cggcatatct ggcaggctgc atcctgcgcc accggaaaga tttgcacaaa ctgtttcatt
    24121 atttgaaaaa acaaggtttc ccattatgaa aatcgttttg accacatcta tggcaggctt
    24181 gggcggcacg gaaaccgcca ccgtccgcct cgggcggctg ctcaaacggc gcgggcacga
    24241 catcatcctt gcttcttcgg acgggccttt tgtcggagag gcgcaagcat cgggcatccg
    24301 ttggcagccg gttgattttt accggggcgg gcttgccggc tacctcaaaa gcacctttgc
    24361 ctacgcccgg atgctgcgcc gcgaacaacc cgacatcatc gactgccaaa tggcgcgcgt
    24421 cgttccagcc tgcgcccttg ccgccaaaat cgtttcgccg aaaaccaaaa tcatctacca
    24481 ctcgcacggt ttggacgcgg caacctaccc caaaatcgcc aagctcttcg acaagctggg
    24541 cgtgtacatc atcggtaact gcaaacacga acgcgaaaaa ctcatccgcc acggtttccc
    24601 cgccgaccgg attgtctacg cctacaacgc actccccccc ccccccccgg aatttccttt
    24661 ctggaaaacc gtcaaaggct gcgtcatgct cggcacgctg tcgcgcctgg acaccgtccg
    24721 cgccgtgcat ctgatgctgg acattttcaa aaaaatggtc gaccgcgata tgcccgtacg
    24781 cctgaacgtg gcgggcatag gcgaagaaat ggacaatttg aaagcgcagg cggaacgtct
    24841 gggcatagac ggcaaagtta ccttcctcgg cggcatacgc gatttgaccg cctacttcaa
    24901 agacgtggat attttggtga acacgcccca ctgcatcggc gaccacggcg caggtgtagg
    24961 caacaacatc ttagaagccg gactttacga cacgcccgtc gttacctacg atatggcggg
    25021 catttccgaa atggtcgtca acggggaaac gggttactgc atccccttcg gcgacgaaga
    25081 agcctttatc gccgccgtag acacgctcgt cagacaaccg gagttgcgcg aagcaatggg
    25141 caaaaggctg cacgcacacg tcgccgcact ctgttccgac gacgaaatct accgcaccac
    25201 catggcggcg tataccatgt aatgccgtct gaaagccgcc gatgaacatc accatagtcg
    25261 ccccttattg ctcgctgccg tccgagccgc acttcaaccg ctttcggtat cttgccgagc
    25321 tgttgtcgca gtcgcatgac gtgctgctga ttaccagcaa cttcaaacac tacgacaaat
    25381 ctttcagacg gcccgaagat gccgaggccg cctcacaagg ccgtctgaaa gtcatgctgt
    25441 tggaagaaag cggctacggc aaaaacgtgt ctttaggacg cttggccagc catcaccgct
    25501 tcgtcagaca ttttgaaaaa tggttggaaa actgccgtcc gggcgaacaa gacatcgtct
    25561 attccgccta tccgctgatt gccaccaacc tgctgttggg caaacacaaa gcacgtttgg
    25621 gctacaaact gattgtcgat gtgcaggacg tatggccgga gtccttctcc tcagtcgtac
    25681 cgtttttgaa aaaagtaccg cacaacctgc tgccctttgc ttcacgtgcc aaccaagcct
    25741 accgctacgc cgatgcgctg gttgccgtat cgcagactta tctcgaccgc gccaaagaaa
    25801 ccaatccgaa cgtacccggc gaagtcgtct atatcggcgc ggattttccc aaacttgatg
    25861 ccgcgcctgc caaagatttt ggcgacgaca aaacccgctt tttctacttg ggcacgctca
    25921 gttacagcta tgacgtggaa accgtgtgca aaggcgttct caagctgctg gacgccggcg
    25981 aaaacgtcga gttgcacatc atgggcggcg gccctgattt ggacaggctc aaacaatacg
    26041 cctgcgacga catcaagttt tacggctaca ttccctatgc cgaaatgatg tcggttgcca
    26101 agggctgcga cattgccatc aacgccatcc agtcctattc aatggcatcg attaccaaca
    26161 aactctccga ctatatggct ttgcaaaaac cgattctgaa cagccaggtc aacgacgaag
    26221 ttgccaaagt ccttaccctg ctgccgtatg cgaactaccg ttccggcgat gtggacggtt
    26281 tcgttcaagc cgccaaagat attttgaagc gcaaaaacga tcctgttcag tccgaagaaa
    26341 tcgtccgccg tttcaagcgc gacgttgcct atcaaaaaat cgtcaacctg attgaaagat
    26401 tagctcatga ataaattttt caaacgcctg tttgacattg tcgcctccgc ttcaggactg
    26461 attatcctgt cgcctgtgtt tttgattttg gtgtacctca tccgcaaaaa cctaggctcg
    26521 cccgtgttct tcatccaaga gcggccgggc aaggatggca agccgtttaa aatgattaaa
    26581 ttccgctcca tgcgcgacgc gcttgattca gacggcattc cgctgcccga cagcgagcgg
    26641 ctcacaccct tcggcaaaaa actgcgtgcc gctagtttgg acgaactgcc tgaattatgg
    26701 aatgtcttaa aaggcgacat gagcctggtc ggcccgcgcc cgctgctgat gcaatatctg
    26761 ccgctgtacg acaacttcca aaaccgccgc cacgaaatga gacccggcat taccggctgg
    26821 gcgcaggtca acgggcgcaa cgccgcctca tgggaagaac gcttcgcatg cgacatctgg
    26881 tatatcgacc acttcagcct gtgcctcgac atcaaaatcc tactgctgac ggttaaaaaa
    26941 gtattaatca aggaagggat ttccgcacag ggcgaagcta ccatgcccac tttcacagga
    27001 aaacgcaaac ttgccgtcgt cggcgcgggc ggacacggca aagtcgttgc cgagcttgcc
    27061 gccgcactcg gcacatacgg cgaaatcgtt tttctggacg accgcgccca aggcagcgtc
    27121 aacggctttt ccgtcatcgg cacgacactg ctacttgaaa acagtttatc gcccgaacaa
    27181 tacgacatcg ccgtcgccgt cggcaacaac cacatccgcc gccaaatcgc cgaaaaagcc
    27241 gccgcgctcg gcttcgccct acccgttctg attcatcccg acgcgaccgt ctcgccttct
    27301 gcaacagtcg gacaaggcag tgtcgtgatg gcgaaagccg tcgtacaggc tggcagcgta
    27361 ttgaaagacg gcgtgattgt gaacaccgcc gccaccgtcg atcacgactg cctgcttgac
    27421 gctttcgtcc acatcagccc gggcgcgcac ctgtcgggca acacgcatat cggcgaagaa
    27481 agctggatag gcacgggcgc gtgcagccgc cagcagatcc gtatcggcag ccgcgcaacc
    27541 attggagcgg gcgcagtcgt cgtacgcgac gtttcagacg gcataaccgt cgcgggcaat
    27601 ccggcaaagc tgctgccgcg caaaaacccc gagaccggga cggcataagc gattaaaata
    27661 tagtggatta aatttaaatc aggacaaggc gacgaagccg cagacaatac aaatagtacg
    27721 gcaaggcgag gcaacgccgt actggtttaa atttaatcca ctatacaccc ccgtacaaac
    27781 cgattttgac aacacctgca gcgtgcgacc gattcatcgg aacacgcaaa acccctccat
    27841 cagacggcat attgtcagaa atgccgtctg aacccgacgg accaaacacc atgctgaaca
    27901 cctccctctc cccgtggccc tgctttaccc aagaagaagc cgatgccgtt tccaaagtcc
    27961 tgctgtccaa caaagtcaac tactggacgg gcagcgaatg ccgcaaattt gaaaaagaat
    28021 ttgccgcctt tgccggcacg cggtacgccg tcgccctttc caacggcacg ctggcactcg
    28081 atgtcgcgct caaagcaatc ggcataggcg cgggcaacga tgtgattgtt acctcgcgca
    28141 ctttcttggc ttccgcttcc tgcattgtga acgcgggcgc aaatcccgtg tttgccgatg
    28201 tggatttgaa cagccaaaac atcagcgccg aaactatcaa agccgcgctg acgtcgaata
    28261 ccaaagccat catcgtcgtc cacctcgccg gtatgcccgc cgaaatggac ggcatcatgg
    28321 acttggcaaa agaacatgat ttgtgggtca tcgaagactg cgcccaagcg cacggcgcga
    28381 aatacaaagg caaatccgtc ggcagcatcg gacacgtcgg cgcatggtcg ttctgccaag
    28441 acaaaatcat gaccacgggc ggcgaaggcg gcatggttac caccaacgac aaagcactgt
    28501 ggagcaaaat gtggtcgtac aaagaccacg gcaaaagcta tgacgccgtg taccaccgcg
    28561 aacacgcccc tggcttccgc tggctgcacg aaagcttcgg cacaaactgg cgtatgatgg
    28621 aaatgcaggc ggcaatcggg cgcattcagc tcaaacgcct gcccgaatgg actgcctgcc
    28681 gtcaagcgca cgccgccaag ctggcggaaa gcctaggcaa attcaaaagt atccgcttgg
    28741 ttgaagtcgc cgactacatc ggacacgcgc aatataagtt ctacgccttc gtcaaacccg
    28801 aacacctcaa agacggctgg acgcgcgacc gcatcgtcaa cgaactgaac gcgcgcaaag
    28861 tcccctgcta tcaaggtagc tgctccgaag tgtatttgga gaaatccttc gacaacacgc
    28921 cttggcgtcc gaaagagcgg ctgaaaaatg ctgtcgagtt gggcgacacc agcctgatgt
    28981 tcttagtaca tccgacgctg accgacggcg aaatcgcgtt ctgcaaagaa cacatcgaag
    29041 ccgtattgac cgaagccaca cgataaccct tcagacggca tatgccgcct gaaagcacat
    29101 accgccccac gatatgactc tggaaaccct gcttgcccta ccgcgcaaca tcaagaaaat
    29161 ctgtttcctc atacacgatt ttctgatgat tttcattgcc ttttggttta cccaaagcct
    29221 gaaggccgac tactcgaacg aatggttcga ccctgccaac tggcagtctt tcctattgac
    29281 cgcgctgttg accatcggcc tgttcgtcag aatggggctc taccgtgccg ttacacgctt
    29341 tgtcagtttc cacgtcctga ccaccgcttt tgccggcagc attgtttccg ccgtcctgtt
    29401 cttcctcaac acattgattt ttgaagaaag gctgcgcctc gccctgccga ttgtctattt
    29461 cttactgctg tttgtttccg tgaccggctc gcgtatggtt ttgcgcggac tgttgtccga
    29521 acaccccaaa aaacagatga tccccgtcat catttacggc gcgggacggt cgggcagaca
    29581 actgcttgag gccgtcaaac aaatgcgcga atattccgcc gccgcctttg tggacgacga
    29641 ccccaaactg tggcacaccg tcatctacga ccttgccgtt taccagcccg atgccatcgc
    29701 cttcctcatc gaacgctacg gcgtggaaaa aatcctgctc gccatccccg gcgcgactca
    29761 ggaacaacgc ctccgaatca tcaacaaact ggaagcctat ccctgcgaag tgttgaccat
    29821 tcccggaatg aaagacctga tggacggcaa actcagcatc ggcacgctca aaaaaatctc
    29881 tgtgtccgac ctgctcgggc gtgattccgt cgcaccagac gaccgcctca tgaatgccga
    29941 catcgaaggc aaaaccgtca tggtaaccgg cgcgggcggc tccatcggtt cggaactctg
    30001 ccgccagatt atccgccgcc gccccgaaaa gctgctgctg ttcgagttat ccgaattcgc
    30061 cctgtacgcc atcgaaaaag aattgcgcga aacctgcatc caaaaacgcc tcgataccga
    30121 aatcctgccc tttctcggtt cggtgcaaaa ccgcacgctg ctcgaacacg tcatgaccgc
    30181 cttttccgtt gcgaccgtct atcacgccgc cgcctacaaa cacgtcccca tggtcgagtt
    30241 caacaccgtc gaaggcatac gcaacaacat cttcggcaca ctcgagtgcg cgcttgccgc
    30301 cacggcatcg ggcgtaagaa ctttcgtcct catctccacc gacaaagccg tccgccccac
    30361 caacaccatg ggtgccggca aacgcatggc ggaactctgc cttcaagcgc tcgccgccga
    30421 acccggacaa aaaacccgct tcagcatggt gcgtttcggc aatgttttag gttcgtccgg
    30481 ctccgttgtc ccgctgtttg aaaaacagat tgcagaaggc ggcccgatta ccctgaccca
    30541 ccaagacatt acccgctact tcatgaccat tcccgaagcg gcgcaactgg ttatccaagc
    30601 cggcgcgatg ggtacgggcg gcgacgtatt cgtcctcgat atgggcgaat ccgtcaaaat
    30661 catcgacctt gcccgccaaa tgattacctt aagcggcctc aaacccaaaa cacccgaaca
    30721 acccgacggc gacatcgaaa tcctcattac cggactgcgt cccggcgaaa aactctacga
    30781 agagctgctc atcggcgaca acgtccgcaa aaccggccat ccgcgcatca tgaccgccaa
    30841 cgagaccatg ctgccgtggc gcgagctctc cgccctgctc gaccgcatcc gtgcggcctg
    30901 cgaccgttac gaccagcagg caatccgcac cctgctcatc aacgccccga ccggctttgc
    30961 cccgagcgac ggtatctgcg acctgctttg ggtacgagaa acacacagaa aaaatgccgt
    31021 ctgaaccttc agacggcata acgtacaaac caacctacct tacacacacg gagtttgaca
    31081 tgcagttttc ggcattcggc gaaaaattca cgcaacacag cggcatcctc caactgatgg
    31141 acgacctcgg cgacgcgctc aaaagcgaca agcccgtcaa catgctcggc ggcggcaacc
    31201 cggcgcgcat tccggaaatc gatcgggcgt ttgccgacat attctccaaa ctggcggcag
    31261 aacacgccgt cgaaaacatc ggcaactact ccaatcctca aggagatgcc gcgctgattg
    31321 acgcgctaac cgccttcctc aaccgcgaat acggctggaa cctgaccgcc gacaatatcg
    31381 cgctgaccaa tggttcgcaa aacgcgtttt tctatttatt caacctcttc ggcggcaaat
    31441 tcaagctttc agacggcaca tccgcagaaa aagccatttt gttgccgctc gcgcccgaat
    31501 acatcggcta tgccgacgtg catatcgaag ggcagcactt cgtttccgcc aaacccaaaa
    31561 tcgaaaacgt cgaacacgaa ggcgaagcag gcttcttcaa ataccgcgtg gactttgacg
    31621 cactggaaaa cctgcccgaa ctcaaagcgg gcaaaatcgg cgcgatttgc tgttcgcgcc
    31681 cgaccaaccc gaccggcaat gtgttgaccg acggcgaaat ggcgcgtttg gacgctttgg
    31741 cgcaggaaca cggcattccg ctgattatcg acaacgccta cggaatgccg ttccccaaca
    31801 tcatttacag cgacgtaacg ctgaattggc atgaaaacat catcctctgc ttcagccttt
    31861 ccaaagtcgg tctgccgggc gtgcgcaccg gcatcatcgt cgccgcgccc gaagtcgtca
    31921 aagccgtcag cagcctgaac gcgattgtga accttgcccc cacgcgcttc ggcgcggcca
    31981 tcgccgcccc gctgctggaa agcggcgaga tgaaacggct tgccgaccaa gtcatccggc
    32041 cgttttaccg caatcaggcg caaaccgccg tctcgctgct caagcgcgag ctgggcgcgt
    32101 acccgatgaa aatccacaaa cccgaaggcg cgattttcct gtggctctgg tttgaaaacc
    32161 tgcccgtttc ttcgcaaacc ctgtacgaaa tactcaaagc cgaaggcacg ctgattattc
    32221 cgggcgagca tttcttcatc ggcatcgaca cgcaggatta cccgcacgcg ggcgagtgca
    32281 tccgcatgag catcgcgcag gacgctcaaa cgctggaaaa aggcattgcc gccatcggca
    32341 aaaccgtccg aaaactgtac gacaacgttt aaaacgcaaa aaaatgccgt ctgaaaagtt
    32401 ttcagacggc atgagacctt tgcaataaca taggttacta aaattttatg ctcaatctca
    32461 ttttcaaaat gcaaagcttt tctgattttt cctacttttt gctcaatatt aggaaggttt
    32521 taggcaattg aaaatttttt ggcgcatttt tatgcgtcaa atttcgttaa cagactattt
    32581 ttgcaaaggt ctcggcattt ttatccgcat tcaatgccgg gaaaaatgtt cccacaccgg
    32641 cctgcagttt tccggcagct cgggacacgc gccgagaatg ccgccgctga aatcgtttaa
    32701 gcggtggaaa tcgctgcccg cgcttgccaa caatccgaag cgttctgcca aaagcgcgta
    32761 gttgaggcgg tcgtttttgc aacagttgcc gctgtggact tcgatgcctg cgccgccgag
    32821 gtttttaaat tcttcaaaca gattgcgctt ggcggtggcg gacaaatcgt agcgcatcgg
    32881 atgggcgatg accgccagcc cgcccgcgcc gttgacggcg gagacgcagt cttccagcgt
    32941 cgcccattcg tgtcggacgg cgcaggattt gccgtcgccc aagtatttgg tgaacgcctg
    33001 ctgcttgttt ttgacatgtc ccgcccggat gaggaactcg gcgacgtggg tgcggctgac
    33061 catttctttg tttgccgcca gcgcgagcgc gccgtcgtat gcgccgccga tgcctttctt
    33121 ttcgagcttg gcggcgatgg cttcgaggcg tttcagacgg ccttgccgca cttgcgccaa
    33181 caggttttgc aggttttcgt cccgttcgtc gaaatccaaa ccgacaacgt gtatggtgcg
    33241 tccgcgccaa gtgacggaga tttccacgcc gttaatcagg cgcaaatcga gcctgtcggc
    33301 ttcggcacgc gcttcggcga tgccgccggt gtggtcgtgg tcggtcaacg ccagcagcgt
    33361 gcagccgttt tgatgcgcga ggcggacgac ttcggcgggg gaaagcatac cgtcggaaac
    33421 ggtggaatgg cagtgcaggt ctatcatggg gtgttatatg tgtggtgaat gaaggtcggg
    33481 gggcaatatt gtcgggcagt gtggcggcgg cggtcatttt aaaaaacagg tgtatggcaa
    33541 atgcaaagaa aaagtcccta taccgtcatt cccgcgcagg cgggaatcca gactttgatt
    33601 tattggaaat gtttaaggtt aaccgctatt tcaaacttcc ggattcccgc ctgcgcggga
    33661 atgacgatag ggatgttttc aattttaatc tactataaaa gactgtctga aaacgtggtt
    33721 ttatagtgaa ttaaatttaa accggtacag cgttggctcg ccttggctca aagagaacga
    33781 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctgcggc
    33841 tcgccgcctt gtcctgattt ttgttaattc actatatcaa gccgaaccgt tttcagacgg
    33901 cattttcgtc ttccgaccaa cctgcttctt tcaatttccg ccgttgcgcg tcgtagcggg
    33961 ccttatctgc ccaataaatc gtctgaatac acgcctcgcc gcagtcgctg ccgcagcatt
    34021 cccacgactc gggtcggacg ggttcgtcta aaagcggctc gcccaaaagg gcttcggctt
    34081 tatgcttcag ggtcatatcc atcggcgatt tccaagcgcg caccgtcaaa ctcgatgact
    34141 tcgccgccgc gtattttggc ggttttgcgg gtttcggttt cgccgttgcg caacaccagc
    34201 ccttcggcaa tcaacgcctt cgcctgtccg ccgctttcgg caagtccgac caatttcaag
    34261 aggtcgcaca aggcgatgta ttcgttgtct tcgagataga cgatggcttc catgatgttc
    34321 ccttgcagaa agagcccgtt attgtagcac ctgccgccgc cgtacccaaa attaccgcaa
    34381 aaccagcgat gtatccgcac cgcctgttcc gtaaaaatgc cgtctgaaac cccgtatgcc
    34441 gccagccggt caaagaaatc ctgtccggcg gcaggctgca aatcctgttc cccgacgaat
    34501 ccacattgac gctgatgcac ctcctcaaac gcgaactgcc cgatacacca gcaatcggca
    34561 tcaaaacgaa atcaaaaacc tgtttgaacg taaaattaat ttaactgctt gccatccgtc
    34621 cgaataaggt atatagttcc ttataactag tttgatagtc ctttatatct atcaatactc
    34681 cttgggaagc ctccgccata cggcaggagg catttttttg ccgtcgtaaa agttcaaaaa
    34741 catttgcagg tcatgccgtc tgaatctgaa acagcattac ctacaccgcc atctaaagac
    34801 aaccctgcta caatacgcct tttattgtcc acgccgattt tgccatgacc gaaccgacct
    34861 acatcccgct gcgcctgcat accgaatttt cgattaccga cggtatggtg cggattaaaa
    34921 aactgattgc caaagcgcag gaatacggtt tgcctgcttt gggcatcagc gatttgatga
    34981 acgaattcgg tttggtgaaa ttttataaag cctgccgcag cgcggggatt aagcctatcg
    35041 gcgcggcgga cgtatggata ggcaatccga atgcgcccga caagccgttt cgcgccatgc
    35101 tgattatccg caacgatgcg ggttatctgc gcttgagcga gcttttgacg gaggcttatg
    35161 tcggcaaaga ccgcaatgtc catcacgcgg aactcaatcc cgaatggctg gacaacggcg
    35221 acaacagcgg cttgatttgt ttgagcggcg cgcattacgg cgaagtgggc gtgaatctgt
    35281 tgaacggcaa tgaagatgcg gcgcgtgcgg cggcgttgaa atatgcggcg tggtttcccg
    35341 atgcgtttta tctggagctg caacgcctgc ccgaacgccc cgaatgggag gcttgcgttt
    35401 cgggcagcgt gaagctggcg gaggaactgg gtttgccggt ggtagcgacg catccgacgc
    35461 agtttatgag ccgcgacgat ttcaacgcgc acgaggcgcg ggtgtgcatc gcgggcggct
    35521 gggtattgac ggacaaaaaa cgtccgcgcg atttcacgcc gagccagttt ttcattccgc
    35581 cggaaaccat ggccgaacgt ttcgccgatt tgcccgaggc cttggaaaac acggtggaaa
    35641 tcgccaaacg ctgcaacctg cacatcacat tgggcaaaaa cttcctgccg ctcttcccta
    35701 ctccggacgg cctgtcactg gatgattatt tggtgaaact gtccaacgag ggtttgcagg
    35761 aacgcatggt tcagctttat cccgacgagg cggagcgtgc ggcaaaaatg ccggaatatc
    35821 aggaacgttt ggattttgag ctgaacatca tcatccagat gaaattcccc ggctatttcc
    35881 ttatcgtaca agactttatc aactgggcga aaacgcacgg ctgtccggtc gggccgggcc
    35941 gtggttcggg cgcgggttcg ctggtggcgt attcgctgaa gattaccgac ctcgacccgc
    36001 tcaaatacgc gctgctgttc gaacgtttct taaaccctga acgcgtttcc atgcccgact
    36061 ttgacgtgga cttctgtcag gcaaatcgcg gccgcgtgat tgaatatgtg cgcgaaaaat
    36121 atggcgcgga agcagtcagc caaattgtta ccttcggcac aatgtcgtcc aaagcggtga
    36181 ttcgcgacgt ggggcgtgtg ctggaactgc cgtttatgct gtgcgacaaa ctgtccaagc
    36241 tgattccgtt ggaagccaac aaacccctga gtttggaaaa agccatggag accgagccgc
    36301 agattcagga attgattgaa gcggaagaag cggacgaact gattacgttg gcgaaaaagc
    36361 tggaagattt aacgcgcggt ctgggtatgc acgcgggcgg cgtgttgatt gcgccgggca
    36421 agatttccga ttacagcccc gtgtatcagg cggacgaatc cgcctcgccc gtatccatgt
    36481 acgataaagg cgacgtggaa gatgtgggtt tggtgaaatt cgactttttg ggcctgcgca
    36541 acctgaccat tatcgaaatg gcgcagaaca acatcaaaag caccaccggc gatatcgtcg
    36601 atgtcggcaa gattccgctt gacgaccagg ttgcctacca aatcttccgc gatgcgaaca
    36661 ctaccgccgt attccagttc gagtcgaccg gcatgaagaa aatgctgaaa acggcgcata
    36721 cgaccaagtt tgaagaactc atcgccttcg tgtcgctcta ccgacccggc ccgatggaca
    36781 acatccccga cttcgtcgcg cgtatgaagg ggcaagaatt ccaatacatc catccgctac
    36841 tcgaaggcat cctcgcgccg acctacggga ttatggtgta tcaggaacaa gtgatgcagg
    36901 cggcgcagat tatcggcggc tactcgctcg gcggtgcgga cctgctgcgc cgcgccatgg
    36961 gtaagaaaaa gcccgaagaa atggtgaaac accgcgaaat cttcgccgaa ggtgcggcaa
    37021 aacaaggcat ttcacgcgaa aaatccgacg aaatcttcaa ctacatggaa aaattcgccg
    37081 gctacggttt caacaaatcc cacgccgccg cctacgccct gatttcctat cagaccgcat
    37141 ggctcaaagc ccactacccc gccgaattta tggcggcgac catgtcgtcc gaattggaca
    37201 acaccgacca gctcaagcat ttctacgacg actgccgtgc caacggcatc gagttcctgc
    37261 cgcccgacat caacgaatcc gactaccgct tcacgccgta tccggacatg aaaatccgct
    37321 acgcactcgg cgcgattaaa ggcacgggcg aagccgccgt cgaatccatc atcgccgcgc
    37381 ggcaaagcgg cggcaagttt accggcctct tggacttctg cgagcgcgtc ggcaaagaac
    37441 acatgaaccg ccgcaccctc gaagccctga tacgcggcgg cgcgttcgac agcatcgaac
    37501 ccaaccgcgc catgctcttg gcaaacatcg acctcgccat gaacaacgcc gaccaaaaag
    37561 ccgccaacgc caatcagggc gggcttttcg acatgatgga agacgccatc gaaccggtgc
    37621 ggctcgtcga cgcgccgatg tggagcgaat cggaaaaact cgccgaagaa aaaaccgtca
    37681 tcggctttta cctgtccggc cacccgttcg gcccgtatgc ccaagaagtc cgccaaatcg
    37741 caccgaccaa attagaccgt ctgaagccgc aagacagcgt gcgcctcgcc ggattcgtta
    37801 ccgccgtgcg tacgatgatg ggcaaacgcg gcaaaatcgc cttcgtcagc ctcgaagatt
    37861 tgagcggaca ggttgaaatc atggtcggcg gtcagacgtt ggaaaactgc gccgactgcc
    37921 tcaaagccga ccaagtgctg attatcgaat ccaaagtcag ccgcgacgac tacggcggcg
    37981 gcgacgggct gcgtattctg gcaaaccaag tcatgaccct gcaaacggcg cgcgaacgct
    38041 acgcccgcag cctcagcctc gccctcgccc cgcatcacga catcggcgga ctggtacaac
    38101 tgcttgccgc ccaccaactg cccgacacgc cgcgcatccc gctgcaactg tcgtatgcca
    38161 acgaaaaagc gtcgggcagg cttcaagtgc cgccgaaatg gacggttacg ccgagttccg
    38221 cattgttcgg cgaactggaa acattgctcg gcagccggtc ggtgcgcgtg aattggtaac
    38281 caaaaataca aatgccgtct gaagcccaaa accggtttca gacggcattt tttaaccgaa
    38341 ataaacgatg ccccttaaag cagaagcttt aaggggcaga gcgttgcggc acatcttttc
    38401 agacggcctt attgtagcaa cggttctcat ttcgcagtgc gacaatatgt ttgggattct
    38461 agcccttgtg agattataag tcaataatct atttgtatat aaataagaca atcggatata
    38521 ttatcattat ttgcaacgtt gtttacggag taataaatag ctgccttcaa acccaatcca
    38581 atcaactaca tcctcggtct cgatatcggc atcgcatccg tcggctgggc gatggtagaa
    38641 attgacgaag aagaaaaccc catccgtctg attgatttgg gcgtgcgcgt atttgagcgt
    38701 gcagaagtac cgaaaacagg cgactccctc gccatggcaa ggcgtttggc acgcagtgtt
    38761 cgccgcctga cccgccgtcg cgcccaccgc ctgcttcggg cccgtcgcct attgaaacgc
    38821 gaaggcgtat tacaagccgc cgattttgat gaaaacggct tgattaaatc cttaccgaat
    38881 acaccatggc aacttcgcgc agccgcatta gaccgcaaac tgacgccttt agagtggtcg
    38941 gcagtcttgt tgcatttaat caaacaccgc ggctatttat cgcaacggaa aaacgagggc
    39001 gaaactgccg ataaggagct tggcgctttg cttaaaggcg tagccgacaa tgcccatgcc
    39061 ttacagacag gcgatttccg cacaccggcc gaattggctt taaataaatt tgagaaagaa
    39121 agcggccata tccgcaacca acgcggcgat tattcgcata cgttcagccg caaagatttg
    39181 caggcggagc tgaatttact gtttgaaaaa caaaaagaat ttggcaatcc gcatatttca
    39241 gacggcctta aagaaggtat tgaaaccctg ctgatgacgc aacgccccgc cctatccggc
    39301 gatgctgttc aaaaaatgtt ggggcattgc acctttgagc cgacagagcc gaaagccgct
    39361 aaaaacacct acacagccga acgtttcgtc tggctaacca aactgaataa cctgcgtatt
    39421 ttagagcaag gcagcgagcg gccattgacc gataccgaac gcaccacgct tatagatgag
    39481 ccatacagaa aatccaaact gacttacgca caagcccgta agcttttgga gctagacaat
    39541 accgcctttt tcaaaggctt gcgctatggt aaagacaatg ccgacgcctc aacgttgatg
    39601 gaaatgaagg cctaccatgc catcagccgt gcgctggaaa aagaaggatt gaaagacaaa
    39661 aaatccccat taaacctttc tcccgaatta caagacgaaa tcggcacggc attctccctg
    39721 ttcaaaaccg atgaggacat tacaggccgt ctgaaagacc gtgtacagcc cgaaatctta
    39781 gaagcgctgt taaaacacat cagcttcgac aagttcgttc aaatttcctt gaaagcattg
    39841 cgccgaattg tgcctctaat ggaacaaggc aaacgttacg atgaagcctg cgccgaaatc
    39901 tacggagacc attacggcaa gaagaatacg gaagaaaaga ttgatctgcc acccatccct
    39961 gccgacgaaa tccgcaaccc cgttgttttg cgtgccttat ctcaggccag aaaagtcatt
    40021 aacggcgtgg ttcggcgtta cggctccccc gcacgtatcc atattgaaac ggcaagggaa
    40081 gtaggaaaat cgtttaaaga ccgcaaagaa atcgaaaaac gccaagaaga aaaccgtaaa
    40141 gaccgtgaaa aagccgccac caaattccga gagtatttcc ccaattttgt cggcgaaccc
    40201 aaatccaaag atattctgaa actgcgcctg tacgagcaac aacacggcaa atgcctgtat
    40261 tcgggcaaag aaatcaactt aggccgtctg aacgaaaaag gctatgtcga aatcgaccat
    40321 gccctgccgt tctcgcgcac atgggacgac agtttcaaca ataaagtgct ggtattgggc
    40381 agcgaaaacc aaaacaaagg caatcaaacc ccttacgaat acttcaacgg caaagacaac
    40441 agccgcgaat ggcaggaatt taaagcgcgt gtcgaaacca gccgtttccc acgcagtaaa
    40501 aaacaacgga ttctgctgca aaaattcgat gaagagggct ttaaagaacg caatctgaac
    40561 gatacgcgct acgtcaaccg tttcctgtgc caattcgttg ccgaccatat gctgctgaca
    40621 ggtaaaggga aaagacgtgt ctttgcctca aacggacaaa ttaccaatct gttgcgcggc
    40681 ttttggggat tacgcaaagt gcgtgcggaa aacgaccgcc atcacgcctt ggacgcggtc
    40741 gtcgttgcct gctcgaccgt tgccatgcag cagaaaatta cccgattcgt acgctataaa
    40801 gagatgaacg cgtttgacgg caaaaccata gacaaagaaa caggagaagt gctgcatcaa
    40861 aaagcacact tcccgcaacc ttgggaattt ttcgcacaag aagtcatgat tcgcgtcttc
    40921 ggcaaaccgg acggcaaacc cgaatttgaa gaagccgata ccccagaaaa actgcgaaca
    40981 ttgctcgctg aaaaattatc ttctcgtccc gaagccgtac acgaatacgt tacaccactg
    41041 tttgtttcac gcgcgcccaa tcggaagatg agcgggcaag gccatatgga gaccgtcaaa
    41101 tccgccaaac gactggacga aagcatcagc gtgttgcgcg taccgctgac acagttaaaa
    41161 ctgaaagact tggaaaaaat ggtcaatcgg gagcgcgaac ctaagctata cgacgcactg
    41221 aaagcacggc tggagacgca taaagacaat cctgccaaag cctttgccga gccgttttac
    41281 aaatacgata aggcaggcaa ccgcacccaa caggtaaaag ccgtacgcgt agagcaagta
    41341 cagaaaaccg gcgtatgggt acgcaaccat aacggtattg ccgacaacgc aaccatggtg
    41401 cgcgtagatg tgtttgagaa aggcggcaag tattatctgg tcccgattta cagttggcag
    41461 gtagcgaaag ggattttacc ggatagggct gttgttgctt ttaaagatga agaagattgg
    41521 acggtaatgg atgatagttt tgagtttagg tttgtacttt atgcgaatga tttaattaaa
    41581 gttcaactta aaaaagacag ctttttaggt tacttttcag gattagatcg agccactgga
    41641 gccatttctc ttcgggagca tgatttggaa aaatcaaaag gtaaagacgg tatgcatcgt
    41701 atcggcgtta aaaccgccct ttcctttcaa aaataccaaa tcgacgaaat gggcaaagaa
    41761 atcagaccat gccgtctgaa aaaacgccca cctgtccgtt aaattaatct atccctgttt
    41821 cagacggcct gaagcaggga tttttatata aaaataaggg gaaaaacatg acttggcgca
    41881 gcctgctcat tcaaaacggc ggcaaactat ccttacaacg ccggcaactg ctgattcaac
    41941 aaaatgacga gtcccacacc gtaccattag aagacatcgc cgttatcatc atcgaaaacc
    42001 gcgaaaccct gattaccgct ccacttctgt cagccttagc cgaacatggc gcaaccttac
    42061 tgacatgtga cgagcagttc ctgccctgcg gacaatggct gccttatgcc caatatcacc
    42121 gccaacttaa aatcctcaaa ctgcagctga atataagcga acccttaaaa aagcaattct
    42181 ggcaacatat cgtccgccaa aaaatcctga atcaagcatt tgtcacagac gaaaccggca
    42241 atgatcttgc agccaaacgg ctgcgcactt tggcatctga agtccgctcg ggcgatacag
    42301 gaaaccgcga agcccaagcc gccgccctct acttccaagc cctctttggc gaaaaattta
    42361 cccgcaatga caacaacgcc gtcaatgccg cattgaacta tacctacgct gtgctccggg
    42421 ctgcagttgc acgcgccctg accttatacg gttggttgcc tgcattaggc ttgtttcata
    42481 gaagtgagtt gaatccattc aatctcgccg atgactttat cgaaccctta cgcccattgg
    42541 ccgatctggt cgttatacat ttgtacgaac aaggccgtct gaaaactgaa ctgacgccca
    42601 gcatcaaaca acaccttatc aaaacccttc actgtcaaac cagcatcgaa cggcaacatt
    42661 tcagcacctt ggctgccatc gacaaaatgg tttcgtcatt tcaagccggc gtaactgata
    42721 aaaacgccaa acaactgaaa ctgcctgaaa tcctaccatt gaaagcatac caatatgagt
    42781 gaggccaaat ttatgaggat tatcgtcttc ttcgacctac cggttatcac ggcggcaaag
    42841 cgcaaagccg ccaatcaatt ccgccagttt ttattaaaag acggatacca aatgctgcaa
    42901 ctttccgtat acagccgtat cgtcaaaggc cgcgattcgt tgcaaaaaca ccacaataga
    42961 ctatgtgcaa accttccgca agaaggctca atccgctgtt tggagataac agaaaagcaa
    43021 tatgccgcca tgaaactgct gttgggcgag ctgaaaaccc aagaaaaaaa ggtcaattca
    43081 gaccaattat tgttatttta agcccatttt ttcagactaa ataaaacggg aaatccttat
    43141 agaataagga tttcccgttg gggtattgta gcactgcgaa atgagaatgg gagctacaac
    43201 cgatacatac gaccatctcg ccaaaaacat attgtagcac tgcgaaatga gaatgggagc
    43261 tacaacccta caacggttgg tttggcgaga aagataattg tagcactgcg aaatgagaat
    43321 gggagctaca acttgattgc gattgtaggg tttgtaggct ggattgtagc actgcgaaat
    43381 gagaatggga gctacaacat ttatttttct gatgagtctg ttatttttat tgtagcactg
    43441 cgaaatgaga atgggagcta caacgtattt tttccgaccc ccctgacccc cttattgtag
    43501 cactgcgaaa tgagaatggg agctacaacc ttgtatttaa ttttgaagtt tttaaattta
    43561 ttgtagcact gcgaaatgag aatgggagct acaacttggc gcaagcaact atgttacgat
    43621 gaaaaattgt agcactgcga aatgagaatg ggagctacaa cgcgttgcgg tcagattaca
    43681 acaatgttga gattgtagca ctgcgaaatg agaatgggag ctacaacttg agtggcaagg
    43741 ggagagtaag atgggaaatt gtagcactgc gaaatgagaa tgggcgctac aaccagccgt
    43801 tgcgataagc gaacgtgcgt taaagtcatt gagacacagt gcaattatgg caagaaaggc
    43861 gacagatatt gtgtcacaga tttgaaataa gaaaaaaccg cccactcaca gagtaggcgg
    43921 aaaacttgcg tttttttgat tgtggtcggg ttagctactg cattgactta acagcaaagc
    43981 aaccgctaaa atgaacttgt ggttcacttc aacggacagc agcaactttt tcccacgcat
    44041 aaggaattta agcattttag cttctcctag tgaagttaat cacacaggcg agagccgaac
    44101 aaaaatcccc gttgcagcgg ggatttttgc tgttccctgt ttgatgatta aactaaaatc
    44161 ttgcaaaatg agacctttgc aaaattcccc aaaatcccct aaattcccac caagccattt
    44221 aggggatttt ccatgagcac cttctttcag caaaccgcac aagccatgat tgccaaacac
    44281 atcgaccgtt tcccgctatt gaagttggat ca
//
LOCUS       DAWFJR010000005        43985 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWFJR010000005 DAWFJR010000000
VERSION     DAWFJR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 43985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43985
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.005"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..166)
                     /locus_tag="WGR04_000211"
     CDS             complement(<1..166)
                     /locus_tag="WGR04_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0793851.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGVNKSTAAYYFHR
                     LRLLIYQNSPH"
     gene            241..1281
                     /gene="murB"
                     /locus_tag="WGR04_000212"
     CDS             241..1281
                     /gene="murB"
                     /locus_tag="WGR04_000212"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ0793852.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADELCDIVRLPEFD
                     RDTVLWLGGGSNILLMQDYAGLVVHMENKGIREIARSDGIVRIEAQAGEIWHDFVLHT
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPTLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPHYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDKQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            1293..1943
                     /locus_tag="WGR04_000213"
     CDS             1293..1943
                     /locus_tag="WGR04_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ0793853.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGVRRTLSLLRPYLLPEHREKYDRKIGNGNP"
     gene            1998..2765
                     /locus_tag="WGR04_000214"
     CDS             1998..2765
                     /locus_tag="WGR04_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0793854.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDTARECDETATPDPQTESARQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVETLFQTAFKPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFAPDDSRGKIIRTVCDNS"
     gene            2825..3391
                     /locus_tag="WGR04_000215"
     CDS             2825..3391
                     /locus_tag="WGR04_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ0793855.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            3411..4301
                     /locus_tag="WGR04_000216"
     CDS             3411..4301
                     /locus_tag="WGR04_000216"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ0793856.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            4326..5084
                     /locus_tag="WGR04_000217"
     CDS             4326..5084
                     /locus_tag="WGR04_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0793857.1"
                     /translation="MNPKISSDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NRWEGDTSRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(5139..5804)
                     /locus_tag="WGR04_000218"
     CDS             complement(5139..5804)
                     /locus_tag="WGR04_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0793858.1"
                     /translation="MAVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(5918..6814)
                     /locus_tag="WGR04_000219"
     CDS             complement(5918..6814)
                     /locus_tag="WGR04_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ0793859.1"
                     /translation="MQLLKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTIARLTMTVYLPAEQKGTHMS
                     RFVALMEQHAEALDFAQLRKLTTEMVALLDSRAGKISVSFPFFRKKTAPVSGIRSLLD
                     YDVCLTGEIKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            6883..8040
                     /locus_tag="WGR04_000220"
     CDS             6883..8040
                     /locus_tag="WGR04_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ0793860.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKVLAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPLDCWLVLRGIKTLALRMNAHCQNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYELAKTQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHDVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(8103..9104)
                     /gene="hemB"
                     /locus_tag="WGR04_000221"
     CDS             complement(8103..9104)
                     /gene="hemB"
                     /locus_tag="WGR04_000221"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ0793861.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEASNLGIPMLALFPVVTANKTECAQEAYNPE
                     GLVPTAVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGTIREALEDTGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALNEVALDIREGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(9287..9571)
                     /gene="yhbY"
                     /locus_tag="WGR04_000222"
     CDS             complement(9287..9571)
                     /gene="yhbY"
                     /locus_tag="WGR04_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ0793862.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEA"
     gene            9681..10301
                     /locus_tag="WGR04_000223"
     CDS             9681..10301
                     /locus_tag="WGR04_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ0793863.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINDKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            10365..12332
                     /gene="ftsH"
                     /locus_tag="WGR04_000224"
     CDS             10365..12332
                     /gene="ftsH"
                     /locus_tag="WGR04_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ0793864.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDKSVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            12554..12985
                     /locus_tag="WGR04_000225"
     CDS             12554..12985
                     /locus_tag="WGR04_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ0793865.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            12978..13256
                     /locus_tag="WGR04_000226"
     CDS             12978..13256
                     /locus_tag="WGR04_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ0793866.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            13309..13887
                     /gene="pth"
                     /locus_tag="WGR04_000227"
     CDS             13309..13887
                     /gene="pth"
                     /locus_tag="WGR04_000227"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ0793867.1"
                     /translation="MSNKIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            13948..14154
                     /locus_tag="WGR04_000228"
     CDS             13948..14154
                     /locus_tag="WGR04_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ0793868.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            14151..14513
                     /locus_tag="WGR04_000229"
     CDS             14151..14513
                     /locus_tag="WGR04_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ0793869.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            14744..16159
                     /locus_tag="WGR04_000230"
     CDS             14744..16159
                     /locus_tag="WGR04_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEZ0793870.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMIPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            16278..16787
                     /locus_tag="WGR04_000231"
     CDS             16278..16787
                     /locus_tag="WGR04_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0793871.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VIIIKAEAV"
     gene            16950..18332
                     /locus_tag="WGR04_000232"
     CDS             16950..18332
                     /locus_tag="WGR04_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ0793872.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDEAAYLIGRAIAAKAAEKDMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIDVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLSIIEKDGFAAAGKQGSVTEKDISGAYHDHI
                     VGHVKLKRPMNIVIDAGNGVGGAFAGKLYRALGCGVTELFCDVDGNFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGLVTKDGNIIYPDRQLMLFAQDVLSRNPGAK
                     VIFDVKSTRLLAPWIKEHGGKAIMEKTGHSFIKSAMKKTGALVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQNISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQAAIERIQNQFKAVI
                     ESNPNLSWPL"
     gene            complement(18352..19107)
                     /locus_tag="WGR04_000233"
     CDS             complement(18352..19107)
                     /locus_tag="WGR04_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0793873.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVGKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLDTMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(19117..19833)
                     /locus_tag="WGR04_000234"
     CDS             complement(19117..19833)
                     /locus_tag="WGR04_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0793874.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(19823..20650)
                     /locus_tag="WGR04_000235"
     CDS             complement(19823..20650)
                     /locus_tag="WGR04_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0793875.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSVPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            20935..21843
                     /locus_tag="WGR04_000236"
     CDS             20935..21843
                     /locus_tag="WGR04_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ0793876.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKTRFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGHDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            21923..25537
                     /gene="recB"
                     /locus_tag="WGR04_000237"
     CDS             21923..25537
                     /gene="recB"
                     /locus_tag="WGR04_000237"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ0793877.1"
                     /translation="MSASIQAFDPLTVPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRGRLDDVLQVLESKGIAKLGDDTLSDGIAAYCAE
                     HHEGDTFLPELLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVEMTEEDGDRLLIPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQISRYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTICRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQIRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAGGRLNFKGYPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALFALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLADNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRLADNAPSGTDFAFTEDAPPPAVYRGNAGQTAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVAAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLITDKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYCLSDIPPECRCPEMGFTLHTEDFGLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICVIDYKSNHLGTDASAYTQQAMDEAVAHH
                     HYYLQALIYAVASARYFKLRRQPPAAVSVRYLFLRGIDGKGGGVWHWDIDAAALEQIK
                     "
     gene            25576..25935
                     /locus_tag="WGR04_000238"
     CDS             25576..25935
                     /locus_tag="WGR04_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ0793878.1"
                     /translation="MNIKQLITAALIASAAFAAQAAPQKPVSAAQTAQHSAVWIDVRS
                     EQEFSEGHLHNAVNIPVDPIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            26067..26546
                     /locus_tag="WGR04_000239"
     CDS             26067..26546
                     /locus_tag="WGR04_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ0793879.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPQTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAAHRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(26818..28197)
                     /gene="radA"
                     /locus_tag="WGR04_000240"
     CDS             complement(26818..28197)
                     /gene="radA"
                     /locus_tag="WGR04_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ0793880.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICREDRD"
     gene            complement(28383..29447)
                     /locus_tag="WGR04_000241"
     CDS             complement(28383..29447)
                     /locus_tag="WGR04_000241"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ0793881.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEADIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAEIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEAARILADYGQGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRHYHGG"
     gene            complement(29522..30739)
                     /locus_tag="WGR04_000242"
     CDS             complement(29522..30739)
                     /locus_tag="WGR04_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ0793882.1"
                     /translation="MKTVVWIVILFAAAVGLALALGIYTGDVYIVLGQTMLRINLHAF
                     VLGLLIAVVVWYFLFKFVIGILNIPEKMQRFSLARKGRKAALYLNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMGNTELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYETVEANLHAAAKMNAGLTRLVRLQLRYAFERGDALEVLAKTEKLSK
                     AGALGESETKQYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGERDQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETC"
     gene            complement(30736..32070)
                     /locus_tag="WGR04_000243"
     CDS             complement(30736..32070)
                     /locus_tag="WGR04_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0793883.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKTIEAPSLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            complement(32085..32825)
                     /locus_tag="WGR04_000244"
     CDS             complement(32085..32825)
                     /locus_tag="WGR04_000244"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ0793884.1"
                     /translation="MLIVRPSGRAKDDVETCRRAEWQAEVLSPIEIETDKASLKRLPE
                     MYARADAVFWVSPTAVETAVPYLNLSDGIKMHISVGQGSRRALERCLGRTVIAPDDGN
                     DSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFRRHKPLNF
                     QNFQTENIAAAYITSAELVQSLFAQVPPQFSRFFKSLLYFTHHPRIAEALKREGVCSV
                     ETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            complement(32864..33286)
                     /locus_tag="WGR04_000245"
     CDS             complement(32864..33286)
                     /locus_tag="WGR04_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793885.1"
                     /translation="MKKMFLSAVLLLSAAAQTVWADTVFPCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGWGRGRWATMKFQNGEFMYTVDAGFDSV
                     THAVGSGVLVERRGKEIARFDCTSKTAQANFDDDDFAW"
     gene            complement(33537..33857)
                     /locus_tag="WGR04_000246"
     CDS             complement(33537..33857)
                     /locus_tag="WGR04_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ0793886.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            complement(33880..34506)
                     /gene="upp"
                     /locus_tag="WGR04_000247"
     CDS             complement(33880..34506)
                     /gene="upp"
                     /locus_tag="WGR04_000247"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ0793887.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(34612..34923)
                     /gene="grxD"
                     /locus_tag="WGR04_000248"
     CDS             complement(34612..34923)
                     /gene="grxD"
                     /locus_tag="WGR04_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ0793888.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(35024..35239)
                     /locus_tag="WGR04_000249"
     CDS             complement(35024..35239)
                     /locus_tag="WGR04_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEZ0793889.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            complement(35265..36044)
                     /locus_tag="WGR04_000250"
     CDS             complement(35265..36044)
                     /locus_tag="WGR04_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0793890.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVAAAASPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYWLFKKVPDMRVV"
     gene            complement(36049..36399)
                     /locus_tag="WGR04_000251"
     CDS             complement(36049..36399)
                     /locus_tag="WGR04_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ0793891.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            complement(36403..37380)
                     /locus_tag="WGR04_000252"
     CDS             complement(36403..37380)
                     /locus_tag="WGR04_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ0793892.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TVSGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            complement(37414..38526)
                     /locus_tag="WGR04_000253"
     CDS             complement(37414..38526)
                     /locus_tag="WGR04_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0793893.1"
                     /translation="MTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            38712..39413
                     /locus_tag="WGR04_000254"
     CDS             38712..39413
                     /locus_tag="WGR04_000254"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ0793894.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGRFSAYEGELAGK
                     RMVLALSGIGKVNAAVSTAWLIHQFAPDCVINTGSAGGLSKGLKVGDVVVGTETAHHD
                     VDVTAFGYAWGQVPQLPARFASDGILIEAAKRAARTFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AANSAKMVAEIVKSL"
     gene            39557..41350
                     /gene="lepA"
                     /locus_tag="WGR04_000255"
     CDS             39557..41350
                     /gene="lepA"
                     /locus_tag="WGR04_000255"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ0793895.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPAGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGIIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPASEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKNGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            41493..42512
                     /gene="lepB"
                     /locus_tag="WGR04_000256"
     CDS             41493..42512
                     /gene="lepB"
                     /locus_tag="WGR04_000256"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ0793896.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPDGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRS
                     GTAIR"
     gene            complement(42731..42880)
                     /locus_tag="WGR04_000257"
     CDS             complement(42731..42880)
                     /locus_tag="WGR04_000257"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793897.1"
                     /translation="MNWQAVKTGKTKHAGNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(42926..43831)
                     /locus_tag="WGR04_000258"
     CDS             complement(42926..43831)
                     /locus_tag="WGR04_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0793898.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFV
                     SGLVSGLMLALSMVFVRKTHEVEPVALFPSMMILSLGGAVSLVVPALLMDGGALYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQSEP"
BASE COUNT        10533 a        12689 c        11092 g         9671 t
ORIGIN      
        1 aatgtggact gttttgataa ataagtaatc gtaaacgatg aaaataatag gctgcggtac
       61 ttttattaac gcctactaac tctgctgctg ttcttgcagt tacacctgcg acaaatagct
      121 caatgagttt attttgttta taccggctta gacgactttt tctcataaag gcaactctaa
      181 cttaatttga atttccctag ttatctagga cagccccaaa catattttaa agttcaaacc
      241 atgcaaccca tccgataccg aaccgacctc accccctaca acaccttcgg tcttcgcgcc
      301 caagcccgag cctttatcgc gctcgaacat gccgacgagt tgtgcgacat cgtccgactg
      361 cccgagttcg accgcgatac tgttttatgg ctgggcggcg gcagcaacat ccttttgatg
      421 caggattacg ccggactggt cgtacacatg gaaaacaaag gcatacgcga gattgcgcgt
      481 tcagacggca ttgttcgcat cgaagcgcag gcgggcgaaa tctggcacga ttttgtcctg
      541 cacaccgtcg cgctgggctt gaatgggctg gaaaacctga gcctgattcc gggtacggtc
      601 ggcgcgtcgc ccgtgcagaa catcggcgca tacggcgtag aggcgaaaga tgtgattcac
      661 agcgtgcgct gcttcgattt ggatacggaa acctttgtaa ccctttccaa tgccgactgc
      721 cgcttcgcct accgcgaaag cctgttcaag caggagggta aagggcgtta tgtgattgtt
      781 tcggtcgtat ttgcattaaa aacgcatttt gtgccgactt tgggttacgg cgatttggcg
      841 gccgccgttg ccgaactgag cgcgggcagg gtcccgacgg cgaaagatgt ttccgatgca
      901 gtgtgtgcaa tccgcaacag taaacttcct aatcctaacg tgcttggcaa tgtcggcagt
      961 ttctttaaaa accccgtcgt cagcgcagaa aaagccgcca ccttgttgca gcggcatcct
     1021 gatatgccgc attatccgca gccggacggt tcggtcaaac tcgccgccgg ctggctgatc
     1081 gaccaatgcc gtctgaaagg cttccaaatc ggcggcgcgg cggtacacga caaacaggca
     1141 ttggttttgg taaacaaaaa caacgcctcg gcaaacgatg tccggcagtt ggcgcaacac
     1201 atcaaattta cagtatttgc tcggtttcag gtagaattgc acgccgaacc caattggctg
     1261 cctgcttcgt tcagcctgta aatccaagga gtatgcccgt gacccgcccc gccaaaatca
     1321 atacttacac acgcatcatc gacgccagcc ttgcgctttt caacgaggaa ggcgagcgca
     1381 acatcagcac caaccatatt gccgcccact tgggcatcag tccgggcaat ctctattacc
     1441 acttccgcaa caaagacgaa atcatcgtcc aactgttcaa acgttacagc gaagccctgc
     1501 tggaatacct caacgaagcc gtgttgccgt ctgatgtgga agactccatc aactatatgg
     1561 cgggcattta cgacgtgatg tgggaatacc gcttcctatt cagcgacgtg aacaccctgc
     1621 ttgcgcgcag tgctgaatta ttgggcgaac acaatacctt cacccaagcc aaagtctccc
     1681 cgctcttggt caacctgctt acccagctca acggcttgaa catcatccaa gccgaccaaa
     1741 ccgctatgaa cgacctcgcc gtcaatatgt ggatggtcac gaaatactgg ttcgacttcg
     1801 acagctccct gcgcggccgc accaagctga ccgaagactc caaagcacgc ggcgtccgtc
     1861 gtaccttaag cctccttcgt ccttatcttt tgcccgaaca ccgcgagaaa tacgaccgga
     1921 aaatcggcaa cggcaacccg taaacccaca gcgtccgaat cagccttcag acggcactta
     1981 cccgacggta tcagcgaatg cgcgcagtcc ccccgcccca tatctccatc acaggcctag
     2041 gttacctcgg cctgccgctg gcacaaaagt tttaccaaca cggcagccgc gtcgccgcca
     2101 taaaacgcag cctgacttcg gacgacatca acctgcccat acatctcgac accttcgacc
     2161 tcggcagcac cgatgccttt cagacggcat tgtggcggca ccacgccgac aaacccgtct
     2221 ggttcttcct gctaccgccc tcctcgctga cgcattacgc cgacaccgtc aaacaatggg
     2281 cagaacttgc ccgggcgtgc aacgtgcaac acctgatttt cacaagcagc accagcgttt
     2341 acggcgatac agcgcgcgaa tgcgacgaaa ccgccacacc cgatccgcaa accgagtccg
     2401 cccgccaaat cctcgccgcc gaacaacacc tgctcgacag cggcatctcc aacatcgaca
     2461 tcctgcgact tggtgggctt tattgcgccg aacgccatcc cgtcagccgt ctcgttcaaa
     2521 agcaaaacat cccaggcggc aaccgcccca tcaacatcgt ccaccgtaat atcgccgtcg
     2581 aaaccctgtt tcagacggca ttcaaacccg gcggcaggcg gctgaaaaac attatcgaac
     2641 cgcgccaccc gacacgacgc gaattctaca cggaagaagc cgccaaactc ggtttgccgg
     2701 ctcctgattt cgcacccgac gacagcaggg gcaaaatcat ccgtaccgtt tgcgataaca
     2761 gctgaagcct gtaaaataag cgtcaacagc aacaaacagg cattctccgc cccgatcaga
     2821 aaatatgtcc gccctcctcc ccatcatcaa ccgcctgatt ctgcaaagcc cggacagccg
     2881 ctcggaactt gccgcctttg caggcaaaac actgaccctg aacattgccg ggctgaaact
     2941 ggcgggacgc atcacggaag acggtttgct ctcggcggga aacggctttg cagacaccga
     3001 aattaccttc cgcaacagcg cggtacagaa aatcctccaa ggcggcgaac ccggggcggg
     3061 cgacatcggg ctcgaaggcg acctcatcct cggcatcgcg gtactgtccc tgctcggcag
     3121 cctgcgttcc cgcgcatcgg acgaattggc acggattttc ggcacgcagg cagacatcgg
     3181 cagccgtgcc gccgacatcg gacacggcat caaacaaatc ggcaggaaca tcgccgaaca
     3241 aatcggcgga ttttcccgcg aatccgagtc cgcaaacatc ggcaacgaag cccttgccga
     3301 ctgcctcgac gaaataagca gactgcgcga cggcgtggaa cgcctcaacg aacgcctcga
     3361 ccggctcgaa cgcgacattt ggatagacta accttcagac ggcatccgac atgaacagcc
     3421 cttttcacaa catcggcatc gtaacccgcc ccaacacgcc cgacatccaa gacaccgcac
     3481 acacgctgat tacctttttg aagcagcacg gctttaccgt ctatctcgac gaagtcggca
     3541 taaaggaagg ctgcatctat acccaagaca ccgtcggctg ccatatcgtc aacaagaccg
     3601 aactggggca atactgcgac ctggtcgccg ttttaggcgg agacggcacc tttctctccg
     3661 tcgcccgcga aatcgccctg cgcgccgttc cgattatcgg catcaaccaa gggcatttgg
     3721 gcttcctgac ccaaattccc cgcgaatata tgacggacaa gctattgccc gttttagaag
     3781 ggaaatacct tgccgaagag cgcatcctga ttgaggccgc actcatccgc gaaggcaaaa
     3841 ccgccgaacg cgccatcgcc ctcaacgatg ccgtcctctc ccgtggcggt gccggacaga
     3901 tgattgagtt tgaagtcttc gtcaatcggg aattcgtcta tacccagcgt tcggacgggc
     3961 tgattgtctc cacccccacc ggatcgaccg cctattcgct tgccgccggc ggccccatca
     4021 tgcaggcagg attacacgcc ttcacgctcg tccccatctg cccacaatcc atgaccaacc
     4081 gccccatcgc cattccagac acgtccgaaa tcgaaatcct cgttacccaa ggcggcgacg
     4141 cgcgcgtcca tttcgacggt caaacccata tcgacgtgca aaacctcgac cgcatcacca
     4201 tccgccgcta ccgaaatccc ttacgcattt tgcacccgac cgactaccaa tatttcaaaa
     4261 ccctgcgcca aaaactgcac tggggcgagc aattagtcta agccggcctt tacccgaaag
     4321 tatccatgaa tcccaaaatt tcctccgacc atacagaaga tgccgtgcgc ctctccaaac
     4381 gcatggcgca actggggctt tgttcacgcc gcgaagccga cggctatatc gaacagggtt
     4441 gggtaacggt caacggcaaa accgccgtac tcggtcagaa agtttcaccg gcagaccgta
     4501 tcgaactgaa caagaaagcc cacgaacagc aggcggcacg cattaccatc ctgctgaaca
     4561 aacccgtcgg ctatgtcagc gcacaagcgg aaaaaggcta taaatccgcc gccgaactga
     4621 ttacccctga aaatcgctgg gaaggcgaca ccagccgcat ccgtttcgat ccgaaacaca
     4681 aaatcggcct agctcccgcc ggcaggctcg acatcgactc ggtcggattg ctggtgttga
     4741 cgcaagacgg ccgtatcgcc aagcagctta tcggcgaaaa cagcggcagt gaaaaagaat
     4801 atttggtgcg cgtgcgcggc aaattggacg aaaaaggact tgccttactg aatcacggat
     4861 tgagtttgga cggcgagaaa ctgcgtcccg cccaagtaga atggcaaaac gaagaccaac
     4921 tgcgcttcgt gttgaaacag ggtaaaaagc ggcaaatccg ccgtatgtgc gaactggtcg
     4981 gactgcgcgt cgtcgggctg aaacgcatcc gcatgggcaa ggtcaaactc ggcaggctgc
     5041 cgcccggcaa atggcgttat ctcgcccccg gtgaatcgtt ttaaataagc attaaaaaat
     5101 gccgtctgaa aatccttgcg ttttcagacg gcattgcctt atgcccaaat aacggtttct
     5161 tccaagggcc tgcgcgcctt cgtggcgatt tcctgaacgc ggtagccgaa tgtcgcggcg
     5221 acggacacgc cccattctgc cgcatcgaac aaaccgaact gcccggacaa tacgcgttcc
     5281 atatcggcat agttgaaacc ttccaccggg caggaatcga tacctgccat cgccgcaccc
     5341 gtcatcatgt tgcccaacgc gatataggtc tggcggcaac accagtcaaa caaggcgcgc
     5401 gaatcgtcca aaatcttgat gtcgtcggct tgaaacgcct gatacctcgc caaagatttt
     5461 gccatggcat ccggttcggt aacgccgcgc cgtttgaggc tttccaacat aaacgggctg
     5521 tcgaagcggg catttttctt tgccaaaaac accaccaaat gactggcggt atccaaagca
     5581 tccgccatac cccaagaaaa cggcttgatt gcctgtcgga tttcggggtt ttgaaccaca
     5641 acaaactgcc aaggctccga accgaccgaa ctgggcgaca aacgcccgag ttctaaaata
     5701 aactgaaaat cctcggcact gattttacgt gccgcatcgt aatgccggca tgatttgcgg
     5761 tttttaaatg cggatagaac ctgctctttg cttaatactg ccatcgtaca tcctttcatt
     5821 acttgtattg caaattgatt tcaaaccgat acggcggcag attcgccaat aacgcccaaa
     5881 cgcctttcag acggcatttg gttcatcgca aacacgccta cgggtaggcg atataggcat
     5941 aagccgaatg gttgtggata gattcgaaat tctcactctc gacgacgaaa ctcttgatgc
     6001 gtttgtcggc aatcagcgaa gtagcgacat cgcgcaccat atcttccacg aatttcgggt
     6061 tttcgtaggc tttttcggta acgtattttt catcggggcg tttgagcagg ccgtagagtt
     6121 ggcagcttgc ctgtgcctcc acataatcga tgacttcctc aataccgact tcggcatcgg
     6181 cagtcaggct gacggtaacg tgcgaacgct gattatgcgc gccatattgg gaaatttctt
     6241 tggaacacgg gcaaagcgag gttacgggaa tcatgacctt catactgtgg ccgtatgccc
     6301 cgtctttgat ttcgcccgtg agacagacat cataatccag taaggaccga ataccggaaa
     6361 ccggcgcggt tttcttgcgg aaaaacggaa aagaaacgct gattttgccg gcgcgggaat
     6421 ctaaaagcgc gaccatttcg gtagtcagct tgcgcaattg tgcaaaatcc aaggcttcgg
     6481 catgttgctc catcaatgcg acaaaacgcg acatatgcgt ccccttctgc tcagcaggca
     6541 ggtaaaccgt catggtcaga cgggcaatgg tggactggat gccttctgca gtttgcaggg
     6601 taatcggaaa gcgcaggtct ttgataccga cctgattgat cggcagattg cgcaaatctc
     6661 ggctggattg cacgtctgca atagtgttca tgagttgttt ttccttaata ttcatgtaat
     6721 tgttgagatg tgtgcatggt atggcgtttc cgccggggaa tataccgtca atctgcaaat
     6781 gcgagattat atcactcgct tttagcagct gcattgattg tttctatttt tcagatagtg
     6841 ataaaatctt cgattattca catctacata ggcagagacc aaatgaaatt cgccacgaaa
     6901 gccattcatt ccagctacga ttgcgacgaa cacaaccgcg cgctgatgcc gccgatttat
     6961 caaaacagta tgtttgcgtt gcacgagatt ggcgaaaatg tgccttaccg ttattcgcgc
     7021 ctgagcaacc cgacccgtca gattttagaa gacaccgttg ccgatttgga acacggtgcg
     7081 gcaggttttg cgttttccag cggtatggcg ggaattgatg ccgtatggcg cactttcctg
     7141 cgcccgggcg ataccattgt cgccgtcgcc gatatttacg gcggcgctta tgatttattg
     7201 gtcgatgttt atcaaaaatg gggggtgaac gttgtttttg ccgatttagg caatccggat
     7261 aatttggacg aactgcttaa agcgcacaag gtcaaactgg tttggctgga aacgccgtcc
     7321 aatccacttt tacgcttggt agacatcaaa gtccttgccg caaaagccaa agcagccggt
     7381 gcgctggtcg gtatcgacaa cacttttgcc acgccgtatc tgcaacagcc gttggatatg
     7441 ggttgcgatt ttgtattcca ttccgctacc aaatatttgt gcggccattc cgacgtgttg
     7501 atgggcatcg tcgttgccaa aaccaaagaa ctggcgcagc ctttgcacga tatgatggtg
     7561 cataccggcg cggttgccgg cccgctggac tgctggctgg tgttgcgcgg catcaaaaca
     7621 ctggctctgc gcatgaacgc ccattgccaa aacgcactcg aaatcgcgcg ccgtttggaa
     7681 gcccatcctg ccattgaaaa agtgttccat cccggcctgc cgtctcacga acattacgaa
     7741 ctggcgaaaa cacaaatgcc caaaggcatc ggcggcgtgg ttacggttta tctcaaaaac
     7801 gacacgcgtg aagcggcaaa cagcgtgatt aaaaacatga aactggtcaa aatggcttcc
     7861 agcctcggcg gtgtggaaag tttggtcaac cattgctatt cccagtccca cagcggcgta
     7921 ccgcatgatg tgaaaatgga aatgggcatc aaagtcggtc tgctgcgttt ctccatcggc
     7981 atcgaagacg cggacgatat ttggaacgat atttccgccg cactcgatac aactttgtaa
     8041 actgtaaaaa tgccgtctga aaccatggtt tcagacggca tttcaattaa cccggccgaa
     8101 aatcaacgct tcaacatctt tgccgcctca atagcgtaat aggtcaaaat cccgtccgca
     8161 cccgcacgtt tgaatgccag caggctttcc aaaaccactt tgccgccgtc cagccagccg
     8221 ttggcaatcg ctgcctgcaa catcgcgtat tctcccgaaa cctgataggc ataagtcggt
     8281 acgccgaact cgtccttcac gcggcggaca acgtccaaat acggtaaacc gggcttcacc
     8341 atcaccatat ccgcaccttc ccgaatatcg agcgccactt cattcagcgc ctcatcggta
     8401 tttgcaggat ccatctgata ggtcttttta tctgccttgc ccaaattgcc cgaactgcct
     8461 accgcatcac ggaaaggacc atagaatgca gaagcatatt tggcggaata tgccataatc
     8521 cgcgtatgga tatgtccggt atcctccaaa gcctcgcgga tggtgccgat acgcccatcc
     8581 atcatatcgg aaggagcaac gacctgcgca cccgcctctg catgacataa agcctgtttc
     8641 accaagactt ctacggtttc atcattcatc acgtaaccgt tttcgtccgt cagtccgtcc
     8701 tgaccgtgaa ccgtataagg atcgagcgcg acatccgtca taatgcccag ttcgggaaac
     8761 ctctcgcgca aggctcggac ggccgtcggc accagtcctt cagggttata cgcctcctgc
     8821 gcacattccg tcttatttgc cgtaacgaca gggaacagtg ccagcatggg tattcccaaa
     8881 tttgacgctt cctccgccgt aaacagcaac ctgtccaaac tttgacgctt cacgcccggc
     8941 atagaaggca catcctcctc gcgcgccgac ccctccaata cgaacaccgg ataaatcaaa
     9001 tcatcggcgg tcagcgtgtg ttcgcgcatc aggcggcgtg aaaaatcatc cctgcgcata
     9061 cggcgcatac gcgaagccgg aacattgcgg taaggaaact gcataagccc tccaatcatc
     9121 ctgtcaacaa ttcaaaccat acggaagccg ccgcaacttg cagacagcat cgggcggcaa
     9181 tcaaaatccg ttttcaccgg aaagggtttc ggcccgccga aatgccgcgc cgaaggacgg
     9241 cgtcccgccc attaaagaac aatagcaaaa caaccgcttc aggctgtcag gcttcgatat
     9301 tcttacgcca caataccaaa agtttcccga tatgctgaac aggttgcgcg tcaaccgcct
     9361 cacataaggc agtgcagatt tcgatacgtt cggcacggtc gtcgccgaat acgcgcactt
     9421 taatcagctc atgcgccgtc aatgccgcat cggtttcctt gatgaccgcg tccgtcagac
     9481 cctgctgacc gaccatcaca acaggatgga gatggtgcgc gcgcgctttc agttccaaaa
     9541 tttctttggt gttcaatttg gtatccgtca ttttcctact gcttgaataa aaagaataat
     9601 gcggcattgt acgcgatttg gcacgacatc ggcaaaatta acgtacaata cgcggtttcc
     9661 attccgaccc gcgaaccact atggctgtac gttccaaatc ctcaaaagcg tggctgcacg
     9721 aacacgtcaa cgaccactac gtccatatgg cgcaaaaaga cggctaccgc gcccgtgccg
     9781 catacaaact tttggaaatc aacgacaaag acaaattaat caaacccggc acggtacttg
     9841 ccgacttggg cagcgcaccg ggaagctggt cgcaggttgc cgccaagctg acgggtactt
     9901 ccggagcagt tttcgccttg gacatcctgc ctatggaagc catagggggc gtctccttca
     9961 ttcagggcga cttccgcgag aacgacgtac tggcacaatt cgaaaccttg ttggacaacc
    10021 gcccgctcga ccttgtaatt tgcgatatgg cgcccaatat gtcgggaaac gccgtaagcg
    10081 atcaggcacg cagcttttat ctgtgcgaac tggctttaga cttcgcctcg caacacctga
    10141 aaaccggcgg cagctttttg gtcaaagtct ttcagggtgc aggctatcag gaatacatgg
    10201 cagccatgcg cgaaattttc ggcacggtgc agacgcgcaa acccgaagcc tcgcgcaatc
    10261 gctccagtga gatttattta ttgggcaaaa ataaacgctg acaatacaga cggcgtgctt
    10321 tacaatcatt tccgttttac acctcaatta tggagccttg ctaagtgggg aacaccttta
    10381 aatcaatcct tgtctgggtc gccttgggta tcggcctgat ggctgcgttc aacgctttag
    10441 acggtaaaaa agaagacaac gggcaaatcg aatattctca gttcatccaa caggtcaaca
    10501 acggcgaagt atccggcgtc aacatcgaag gatccgtcgt cagcggctac ctgattaagg
    10561 gcgagcgcac cgacaaaagc accttcttca ccaacgcgcc tttggacgac aacctgatta
    10621 aaacactgct cgacaaaaac gtccgtgtaa aagtaacgcc ggaagaaaaa ccgagcgcgc
    10681 tggctgccct gttttacagc ctgctgcccg tcctgctgct gattggcgcg tggttctact
    10741 ttatgcgtat gcagacgggc ggcggcggaa aaggcggcgc attctcattc ggcaaaagcc
    10801 gcgcccgcct actggacaaa gatgccaaca aagttacctt tgccgatgtc gccggctgcg
    10861 acgaagccaa agaagaagtg caggaaatcg tcgattacct caaagcgccg aaccgctatc
    10921 aaagcctggg cgggcgcgtg ccgcgcggca tcctgctggc gggcagcccg ggtacgggta
    10981 agacgctttt ggcgaaagcg attgcaggcg aagccggcgt gccgttcttc agcatttcag
    11041 gttccgactt tgtcgaaatg ttcgtcggtg tcggtgcaag ccgcgtccgc gatatgttcg
    11101 agcaggcgaa gaaaaacgcc ccctgcatca tctttatcga cgagattgac gcagtcggcc
    11161 gccaacgcgg cgcaggtttg ggcggcggca atgatgagcg cgagcaaaca ttaaaccaat
    11221 tgttggttga aatggacggt tttgagagca atcagactgt aattgtgatt gcggcaacca
    11281 accgccccga cgtactcgat cctgcgctgc aacgccccgg ccgtttcgac cgccaagtgg
    11341 ttgtccccct gccggacatc cgggggcgcg aacagatttt gaacgtccat tctaaaaaag
    11401 tgcctttgga caaatctgtg gatttattgt ccctcgcgcg cggcacgccg ggtttttccg
    11461 gcgcggattt ggcgaacttg gtcaacgaag ccgccctgtt tgccggccgc cgcaataaag
    11521 tcaaagtcga tcagagcgat tttgaagacg ccaaagacaa aatctatatg ggtccggaac
    11581 gccgcagtat ggtgatgcac gaagacgaaa aacgtgcgac ggcgtatcac gaatccggac
    11641 acgcgattgt tgccgaaagc ctgcccttta ccgaccccgt ccacaaagta accattatgc
    11701 cgcgcggacg tgcgctgggt ctgacttggc agcttccgga gcgcgaccgc atcagtatgt
    11761 ataaagatca gatgttgagc cagctctcca tcctgttcgg cggacggatt gccgaagaca
    11821 tcttcgtcgg acgcatctcc accggcgcat caaacgactt tgaacgcgca acccaaatgg
    11881 cgcgcgaaat ggtaacgcgc tacggcatga gcgacaaaat gggcgtgatg gtttatgcgg
    11941 aaaacgaagg cgaagtcttc ttgggacgca gcgtaacccg ttcgcaaaac atttccgaga
    12001 aaacccagca ggacatcgac gcggaaatcc gccggatttt ggacgagcaa tatcaggttg
    12061 cctacaaaat cctcgatgaa aaccgcgaca agatggaaac gatgtgcaaa gccctgatgg
    12121 aatgggaaac catcgaccgc gatcaggtac tggaaatcat ggcgggcaaa caacccagtc
    12181 cgcccaagga ttacagccac aacctgcgcg agaatgcgga cgcggcggaa gataacgcgc
    12241 cgcacgctcc gactcgggaa gaaaccgaag cacctgcccc ggcagacacc gcttcgacag
    12301 agtccgagca gcagcctgaa aacaaggctt aacttcccga acaaacggca gcccgcaagc
    12361 tgccgttttc ccatcctgtt ttcagacggc attttccact caatgccgtc tgaaacgcca
    12421 aagccgtatc ggtttttacg aatctgcgat atggcggttt aaattgaaat cagaacgggg
    12481 caaggctgta ccggtttaaa attaaaccgc tataataccg cccttcgaat acacgcccgc
    12541 attggtgatg cccgtgaaaa aagttgaaaa aaacatcttg gtgctgcacg gcgcggacaa
    12601 aatgttcgag ctggtcgaca aggttgagga ttacccgcac tttctgccgt ggtatagcaa
    12661 aaccgaggtc atcggacgta gcggcaacga actgaaggca cggctgttta tggactatat
    12721 gcacgttcgc caatcgtttg ccacgcacaa ccgcaacatt ccgggcaggg aaatccgtat
    12781 ggaactgctc gagggtccgt tcaaaacctt acgtggaacg tggaaattta tcgatttggg
    12841 cgacgatatg tgcaaaatcg aattcaattt ggaatacgat ttttccaatg ccgttttgtc
    12901 cgccttaatt tcccccgtct tcaaccacct ttccgccacg ctggtcgaag cgttcgtcaa
    12961 agaggcagac cgccgttatg cttgaaattg agattgtgta cgggctgccc gaccgacagg
    13021 ttttgaaaac catgcagctt gccgagggaa caaccgtccg cgccgccgca ctgcaaagcg
    13081 gtttggacgg catatttgaa gatttaaacc tgcattccgc gcctttgggc attttcggca
    13141 aagccgtcaa agacgacacg cccctgcgcg acggcgaccg catcgaagtg taccgcccgc
    13201 tgttgatcga tcccaaagaa gcgcgccgca aacgcgttca aaatcaagaa gaataaccat
    13261 gctgtctgaa gccttcagac agcattcgac agaaacacgg aaaatatcat gtcaaacaaa
    13321 atcaaaatgg ttgtcggctt gggcaacccc ggcaaagaat acgaacaaac ccgacacaat
    13381 gcgggttttt ggttcctcga cgaactggcg tggaaatgga aggcttcatt taaagaagaa
    13441 aaaaaattct tcggcgaagt cgcccgtgcc gccctgcccg acggcgacgt ttggctgctc
    13501 aaacctgcca cgttcatgaa ccgttccgga caggcagttg ccgcgcttgc acagttctac
    13561 aaaatcaaac ccgaagaaat cctcgtcgtc cacgacgaac tcgacattcc ctgcggacga
    13621 atcaaattca aactcggcgg cggcaacggc ggacacaacg gcttgaaaga cattcaggca
    13681 aaactcggca cggcagacta ttaccgcctg cgcctcggca tcggccaccc gggcgaccgc
    13741 aacctcgtcg tcggctatgt cctgaacaaa cccagtacgg aacaccgccg acagattgac
    13801 gatgccgtcg ccaaatccct gcaagccata cccgacatcc ttgccggcaa atgggaagaa
    13861 gcaacccgct tcctgcacag caaatgaccc gatgccgtct gaagcccttt cagacggcat
    13921 gttcccgatt tccatatccg aacagtcatg accgaactca agcagcttat ccaaaccgaa
    13981 tccatccccg tcatcgaaga aaccctcgat ttcctgctgt acgaatgcag catcgacgac
    14041 gcaccatccg ccgaagaagt ggcacaatgg cgcgacatac ttgccgcacg cggcggcaaa
    14101 ttcctgcgcc tgtccaaaat ctgccaaacg tggctggacg aggaggcggc atgaagctgc
    14161 cgcgcaaccg cttcagcctg ctttccgcat tgtggtttgc cggcggcatc tattcgctgc
    14221 tcttcaaagc tgccgacacc gcgccgccgc cgtttccgca tttcgacaaa gcagcacacc
    14281 ttgccctgtt tttcgcacaa atctggcttt tgaccaaagc attcaaaacc ggaaaacttc
    14341 ccatccccta ccgcagcctg atggtctttg ccctctgttt cgccctcttc agcgaatgcg
    14401 cgcaggcatg gtttaccgca acgagaaccg gcagtttggg cgatgttctt gccgatatgg
    14461 caggtacggt tctcgcactc tttgccgccc gcgccgccga ccgcccggac tgaatcggtt
    14521 tatttccccc aaacaggatg cacctcttcc cgatacatcc tatttttccc cttacatatc
    14581 aatcccatct cataaacaaa accgacaaat cgtttacaat atatttacac tacatcagat
    14641 tacaaatata ctcgaaccag ttgcagaatc ggcggttcca caaaccgttt cggattgcac
    14701 gacacaaaag aaaaaccgat tttattgcct taaaggagta ttcatgaacc tgcatgcaaa
    14761 ggacaaaacc cagcatcccg aaaacgtcga gctgctcagt gcgcagaagc cgattaccga
    14821 ctttaagggc ctgctgacca ccattatttc cgccgtcgtc tgtttcggca tttaccacat
    14881 cctgccttac agccccgatg ccaataaagg tatcgcgctg ctgattttcg ttgccgcact
    14941 ttggtttacc gaagccgtcc acattaccgt taccgcgctg atgatcccta tcctcgccgt
    15001 cgtactcggt ttccccgaca tggacatcaa aaaggcgatg gctgattttt ccaacccgat
    15061 tatctacatt tttttcggcg gcttcgcgct tgccaccgcc ctgcatatgc agcggctgga
    15121 ccgtaaaatc gccgtcagcc tgttgcgcct gtcgcgcggc aatatgaaag tggcggtttt
    15181 gatgttgttc ctcgttaccg cctttctgtc catgtggatc agcaacaccg ccaccgccgc
    15241 gatgatgctg cctctagcaa tgggtatgct gagccacctc gaccaggaaa aagaacacaa
    15301 aacctacgtc ttcctcctgc tcggcatcgc ctattgcgcc agcatcggcg gcttgggcac
    15361 gctcgtcggc tcgccgccca acctgattgc cgccaaagcc ctaaatctgg acttcgtcgg
    15421 ctggatgaag ctcggcctgc cgatgatgct gttgattctg cccttgatgc tgctctccct
    15481 gtacgtcatc ctcaaaccta atttgaacga acgcgtggaa atcaaagccg aatccatccc
    15541 ttggacgctg caccgcgtga tcgcgctgtt gattttcctt gccacagccg ccgcgtggat
    15601 attcagctcc aaaatcaaaa ccgccttcgg catttccaat cccgacaccg ttatcgccct
    15661 gagtgccgcc gtcgccgtcg tcgtcttcgg cgtggcgcaa tggaaggaag tcgcccgcaa
    15721 taccgactgg ggcgtgttga tgctcttcgg cggcggcatc agcctgagca cgctgttgaa
    15781 aacatccggc gcgtccgaag ccttgggaca gcaggttgcc gccacctttt ctggcgcgcc
    15841 cgcatttttg gtgatactca tcgtcgccgc cttcattatt tttctgaccg agttcaccag
    15901 caacaccgcc tccgccgcat tgcttgtacc gattttctcc ggcatcgcta tgcagatggg
    15961 gctgcccgaa caagtcttgg tattcgtcat cggcatcggc gcatcttgtg ccttcatgct
    16021 gccggttgcc acaccgccta acgcgattgt gttcggcacg ggcttaatca agcaacgcga
    16081 aatgatgaat gtcggcatac tgctgaacat cctctgcgta gtattggttg ctctgtgggc
    16141 ttatgctgta ctgatgtaaa ccatcgacct aaacaacaag accgtctgaa agaatatttt
    16201 tcagacgacc ttgaagtttg tcgttcagac acaatttgtc gaatcattca aaaccagatt
    16261 ctaacgaaag gaaacccatg attatcctgc acaccaacaa aggcgacatc aaaatcgaac
    16321 tcgatttcga caaagccccc gttaccgcca aaaacttcga gcaatacgtc aaagacggct
    16381 tctacgacgg cgtaatcttc caccgcgtca tcaaaggctt catgattcaa ggcggcggca
    16441 tggatgaaaa catgaacgaa aaagaaaccc gcgatccgat tcaaaacgaa gcgtccaacg
    16501 gcctgcccaa cgataaatac accatcgcca tggcacgcac ttccgacccc cattccgcca
    16561 gcgcgcaatt cttcatcaac actgccgaca acgctttcct gaacttccgt tctaaagagc
    16621 tgtacggcaa aaccgtcgtc caagactggg gctatgcagt attcggtaaa gtcgttgacg
    16681 gttttgacgt tgtcgatgcc atcgaaggcg tttctaccaa acgtcatggt taccacgacg
    16741 acgtaccgag cgaacctgtc atcatcatta aagccgaagc ggtataaacc ggcaatccga
    16801 aagcagcctg cacaaaggct gctttttttg tttcaggcgg cataaaatcc aagcacatat
    16861 gccgtctgaa cacctcccgc aagccttcgg acaatgttgc aacgtgttaa aatagcctcc
    16921 tttcctgaaa ccacaacaag gaaaccatca tggcaaatat tgcccgcgac atcttcaaag
    16981 cctacgacat ccgtggcatc gtcggcaaaa ccctgaccga cgaagccgcc tatctcatcg
    17041 gcagggccat cgccgctaaa gccgccgaaa aagacatgac ccgcatcgcg ctcggacgcg
    17101 acggacgctt gagcggtccc gaactgatgg agcacatcca acgcggcttt accgacagcg
    17161 gcatcgacgt cctcaacgtc ggtatggtcg ccacccccat gctctatttt gccgctatca
    17221 acgaatgcgg cggcagcggc gtgatgatta ccggcagcca caatccgcct gattacaacg
    17281 gtttcaaaat gatgctcggc ggtgacacgc ttgccggcga agccatccaa gagcttttgt
    17341 ccatcattga aaaagacggt tttgctgccg ccggcaaaca aggcagcgta accgaaaaag
    17401 acatctccgg cgcgtaccac gaccacatcg tcggacacgt caaactcaaa cgcccgatga
    17461 acatcgtcat cgacgcgggc aacggcgtgg gcggcgcgtt tgccggcaaa ctctaccgcg
    17521 ctttggggtg cggtgttacc gaacttttct gcgacgtgga cggcaatttc cccaaccacc
    17581 atcccgaccc atccaaaccc aaaaacctgc aagatttgat tgccgcgctg aaaaacggcg
    17641 atgccgaaat cggcctggca tttgacggcg atgccgaccg cttgggcctg gttaccaaag
    17701 acggcaacat tatttatccc gaccgccaac tgatgctgtt cgcgcaagac gtgttaagcc
    17761 gcaatcccgg cgcgaaagtc atcttcgacg tgaaatccac ccgtctgctt gcgccttgga
    17821 ttaaagaaca cggcggcaaa gccataatgg aaaaaaccgg ccacagcttt atcaaatccg
    17881 ccatgaaaaa aaccggcgcg ctggttgccg gcgaaatgag cggacacatc ttcttcaaag
    17941 aacgctggtt cggcttcgac gacggtctgt acgccggcgc acgcctcttg gaaatcctgt
    18001 ccgcctccga taatccgtcc gaagtgttga acaacctgcc gcaaaacatt tccacgcccg
    18061 aactcaacat cgccctgccc gaaggcagca acggccatca ggttatcgac gaactcgccg
    18121 ccaaagccga attcgaaggc gcaaccgaaa tcatcaccat cgacggtctg cgcgttgaat
    18181 ttcccgacgg cttcggtctg atgcgtgctt ccaataccac gccgattcta gtgttgcgtt
    18241 ttgaggcgga tacgcaagca gccatcgagc ggattcaaaa ccaattcaaa gccgtcatcg
    18301 aaagcaatcc aaatttaagc tggccgctgt aaacacagga aaaatgctta atcaaatctt
    18361 ggtagattgg atttggctta aaaatctccg cgtccgttcg tgtttggggt ggtcgaacaa
    18421 ctctttcggg ctgccctgct ctacgataac gccgccgtcc atgacgacga cggtcgtggc
    18481 aacttccagc gcgaacttga tttcgtgggt aacgacgacc atcgtccaac cttcccgcgc
    18541 caattccttc atggtatcca aaacatcttg caccaattca gggtcgagcg cggaagtcgg
    18601 ttcgtcaaac aacatcagct cgggctgaat cgccaatgcg cgggcaatgc cgacgcgctg
    18661 ctgctgaccg ccggaaagct ggtagggata caaatccact ttgtcgccca agccgacttt
    18721 ttccagcagt ttcagagcct cttcgcgcgc ttgggcggca ggcttgccct gtacggcaac
    18781 cggtccttcc attacgtttt ccaaggcggt tttgtgcgga aagaggttgt attgttgaaa
    18841 caccatgcct gatttgcggc gcagtgccaa aatatcgtgt ttgcttggtt ttttagaaaa
    18901 atcgattttc agcggtcgct cgttgtcgaa ctcgatttgt ccgtcttcgg gcatttccaa
    18961 cgcgtttagg catcgcagaa acgtcgtttt gcctgagccg gaaggcccga ggataacgac
    19021 cacctgccct ttgcccacat ccaaatcgat gccgcgcaaa atagtgtttt cgccgaaggt
    19081 cttatggata ttgcggattt taatcataac aactccttat ttggcgacat aacggtcgaa
    19141 acgtttttcc aaacgtgcct gaatcaggaa cagaaccttg cagaaacacc agtaaaccaa
    19201 tgcggcttcg atatagacgg gcaaaaagtc gtaagtgcgg tttgccgttt cctgcgcgac
    19261 gcggaacaat tcagttaccg ttaccaccgc tgcgagcgag gtgtttttga acaagccgat
    19321 aaactcgttg ctcaaaggcg gcacggcaac gcggaatgcc tgcggcgcga cgatgcggcg
    19381 gaacgtctgc atataggtca taccgatgga gaaacctgct tcccattggc ctttaggtac
    19441 ggacaaaatt gccgcgcgta tggtttcgga agcgtatgca ccgacattga gcgagaagcc
    19501 gataatggcg gcgggaatcg gatcgatata gataccgacg gacggcagcc cgtaaaacac
    19561 aatcacaagc tgcaccaaca gcggcgtacc gcgaatgacg gaaatataaa attccaccaa
    19621 tttcagcagg attttccgca cgatgccgcc ggcgggcata atccgcacca aagccacggc
    19681 taccgcaatc atcataccga taacgaaaga agctgccgcc aaaggcagag acaccgcgaa
    19741 gccggctttg accataggca aaaacgcgct gacaatcata tcggcgcgtg tttccgtcat
    19801 aaacggcagc gaagcaagga aattattgaa cactgatgtc ttttccgaag aattgttcgc
    19861 ccagtttttt cagcgtaccg tcggctttca gctcgttgat tgccgtactg aatttcgcca
    19921 cggcttcgtc attgcccttg ttgacaatca ggccggaacc gactttttca tcggcaggtg
    19981 cggaccaaac gattttcacg cccgcattcg ggtttttctt cagatagtcc aaaaccgcca
    20041 attcgtcgtt cagggttgca tcggcacgtt tttgttcaat cagggtcagc gattgcgcca
    20101 aaccgtcaac agccaccaaa tctgcgcctg cagctttggc tttttcgccg tagttgctgg
    20161 tcagggattg tgcggttttc acgcctttga tgtcgtcgat agatttgatg ttgctgtcgt
    20221 tacgggcaac caatacggca ccgctccagc tgtaaggatc ggatttgtcg aatgtcgctt
    20281 ggcgttcggg gctggtcaga ccgacttggt ttgccaccac gtcgaaacgc cccgccttca
    20341 aacccgccat catcgaatcc cattgcgttt ctttaaactc gactttcacg cccagttttt
    20401 ccgccacggc gcgggttact tccacatcgt aaccggtcag tttgccgtcc ttgtcgtggt
    20461 aggtaaacgg tgcgtaagtg ccttccgtgc cgacggtaac cgtgccttta ttgttgatgc
    20521 gctcgattaa agaaccggaa actgccgatt gtgcaggcac ggaagatgct ccgctgccgc
    20581 cttccgaacc accgcaggcc gccaaaacca atgcggcaat accgccgagt acgaattttt
    20641 tcaacatcat tctctcctgt aaaaagtgtg caagtgcggc attctaagtg aaattgcggc
    20701 aaacacaaag aatacttttt ttatatggtt attccaaatc ggaatggaaa agatatgccg
    20761 aagccgccgg aagcggaaat aaagggggcg ggaaaggcgg cgggcggcac gaaaccgaaa
    20821 agcggcatca ggtatgcaaa cggcggattc acgcacgccg ttccggcaaa aatccgcctg
    20881 ccgattcggt gctataatcc atccgaaaca gatgacacca aaaaggaaca tctcatgaac
    20941 acgccccaat ccgccatcat tcccgaccac gcccaagccg gcatctttat cgaagccgac
    21001 ttcgccgcca accgcctcaa cgatattaaa gccgcctgcc gcgcttcgct cgacgcgtta
    21061 tccgccttga aaacccgctt tccggacgat attttgggtc tgaccatcgc cttcggcagc
    21121 aaagcctggg caacattcgg acatacggac gaaggcagtg aaatcaaacc cttctccgaa
    21181 atgggcaacg ggcttgcgcc gtccacgcag cacgatatgt ccatccacat ccaatctttc
    21241 cgccaaaacg ccgcctacgc gcttgcccaa tccgttttgg gcgcgttcgg cgacagcata
    21301 tgcgtcgcgt ccgaagaaca cggtttgcgc ctgtatcagg atcgcgggct ggacggtttc
    21361 gtcgacggca cggaaaaccc gcagggcgat gaaaatgtcc gcgaagtcgc catcatcccc
    21421 gaaggaaaac ccgatgcggg cggcagctat gtcctgctgc aaaaatacct gcacgatttg
    21481 aaaaaatggg atgccgtccc cgtcgccgaa caggaggcct cggtcggacg cggcaaggaa
    21541 accgacgacg aattcggcca cgatgtccgc ctgcccgatt cgcacctcgg ccgcgtcaac
    21601 ctgaaagaaa acggagtcgg cctgaaaatc gtccgccgca gcctgccctt cggcaaaatc
    21661 agcggcgaac acggtttgat gtttaccgca tactgccgca cgctgcacaa tatcgaagcg
    21721 caattattga gtatgttcgg cgacacggat ggcaaaaccg acctgctgct ccggcacctc
    21781 tccgccgccg tttcgggcgg atactactac gcgccgtctg tcgaacgcct gcaaaacctc
    21841 taagccagac gatgccgtct gaaacccttt ttcagacggc attttcccgt tttaccgtcc
    21901 aaccgttttc cgagcgcgca ctatgtctgc aagcatccaa gcattcgacc cgctaaccgt
    21961 ccccatttcc ggcaccaacc tgattgaggc ctctgccggc accggcaaaa cctacggcat
    22021 tgccgccctg tttacacgcc tgatcgtatt agaacaaaaa aacgtcgaac gcgtattggt
    22081 cgtaaccttc accaaagccg ccaccgccga gctgaaaaca cgcctgcgcg ggcgtttgga
    22141 cgatgtgtta caagttttag aaagcaaagg aattgccaaa cttggagacg acacgctttc
    22201 agacggcatt gccgcctact gcgccgaaca ccacgaaggc gacaccttcc tgcccgaact
    22261 cttaaaacag gctttgcaaa aagagggccg gacgcgtctg attgtccgcc tcaaggccgc
    22321 catcgggcaa ttcgacaacg ccgccatcta taccatccac ggcttctgcc agcgcatcct
    22381 gcgcgactac gccttcctgt gccaagcacc gttcgatgtc gaaatgaccg aagaagacgg
    22441 cgaccgcctg cttatcccgg cgcaagattt ttggcgggaa cgcgtcagca atgatccggt
    22501 gcttgccgca ttggcgttta aacgcaaagc tgtgccgcaa accgtccttg cccaaatttc
    22561 gcgctatctt tcccgcccgt acctgaattt ccgccgtccg caggcggatt tgaaacaggc
    22621 gcagcgcaac gccgaaacct catggcaaac catctgccgc ctgctgccag aactggaagc
    22681 cggcttttgg cgcattcatc ccgacctcaa cggcaacagt taccgcaaaa acagcttcgg
    22741 caaccttttt aaagaactgg cacaaaaatc cgccgccgga caactgccct ttctggacaa
    22801 agacacacac gacagactct tgaaacttgc atccgacaaa ctcgaagccg gactgaaaaa
    22861 aggcaaaacg cccgatgcgg cagtatttgc cgaattgcag aaactggcag acttcgggcg
    22921 cgatttgaac gcactcgaag aagcggaaga agcaacaatg atccggctgc aactggattt
    22981 aatcgaatac ctcaaccgca gccttgccga gatgaaaaaa tcgcgccgcg aacgcggttt
    23041 cgacgacctg ctgctcgatg tccacaccgc gctgaccgac aatccgcacg ccgaaaccct
    23101 cgcccgcgcc gttgccgaaa actgggaaac cgcgctgatc gacgagttcc aagacaccga
    23161 cccgctgcaa tacgaaatct tccaaaaaat cttcatcgcc caaaaccgac cgctgttcct
    23221 cgtcggcgat cccaaacagg cgatttacag ctttcgcgga gcggacattt acgcctacct
    23281 tcaggcggcg gaagatgcgc ggcaccgcta cacgctcgcc accaactacc gcagccacgc
    23341 cgcgcttatc ggcagcatag gcgcgctgtt ccgcctcaaa gaacgcccgt tcgttttgga
    23401 aaacatcggc tattcggaag tcggtgcggc gcgtgccgaa agcaggttgt cgcccaaacg
    23461 tcctgccgta cagatccgtt ggctgcacga aaacgacaat gaaaaagcca acaaagacgt
    23521 tttgcgccgc cgtgccgccg actattgcgc cgacgaaatc gcccacgcgc tcaacgaagc
    23581 cgccggaggc cgtctgaatt tcaaaggcta cccgttgcag tcgggcgata ttgccgtgct
    23641 ggtccgcacg cacaacgagg cggtgatggt ttccgccgcc ctgaaaaaac ggcaggtgca
    23701 aagcgtcctg ctttcgcgcg aatccgtgtt cgcctcgccc gaagccgccg cccttttcgc
    23761 actcatcggc ttctggctcg aaccgcgccg cgccggaacg ctgcgctttg tcctgacaag
    23821 cggcatattc ggttatgacg cgcagcaatt gcacgacttc aaccaaaacg aaagcgagat
    23881 tttgcattgg gcggaatctg cccgaaccgc gctcgacaac tggaatcaat acggcatttt
    23941 cgccgccatg cagcaatttt cccaaacaca cggcatcgaa acgcgcctct taagccggaa
    24001 caacgggcgc agcctgacca attatttcca actgctcgaa ctgcttgccg ccgaagacgc
    24061 ccaaaaccgc aaccccgccg cgctgcacaa atggctgcgc gaccaaatca gcctagccga
    24121 caacaacggc ggcgacaacc gcgccatccg tctggaaagc gacgaagatt tggtcaaaat
    24181 cgttaccatg cacgcctcga aaggtttgca gtatccgctg gtgtactgcc cgtttgcgtg
    24241 ggacgcgcaa gataccgggc cgtccgactg gcaaatcctc caccaaagcg ccaaccgaac
    24301 cgaactgttg gcaaaggcgc aactgtcgga agacgaacag aagcaatacg ccgatgagga
    24361 aatggcggaa cgcctgcgcc tgctttatgt cgcgctgacg cgtgcagagg aacagctcaa
    24421 catctacgcc gcctattctt ccgataccgc cgacaacccc ctcgcctacc tgatcgaagg
    24481 cttgccgcaa gacagccgcg aaaccgtccg ccgtgcctat gcgtgtgaaa aagacggcat
    24541 cgcgatgctc aaacgcaact ggcggcgttt ggcggacaac gccccgtccg gcacggattt
    24601 cgccttcaca gaagatgcgc cgccgcctgc cgtatatcgc ggcaacgccg gtcaaaccgc
    24661 cgaatttgcc gcaaacagca ttcccgaacg cggattccga tttgtccgcc acaccagctt
    24721 caccgcctta agccgccata cccaaacgcc cgacggcggc gaagaagatg cctgcccgtc
    24781 cttggatgcc gccgaaacct cggtagcggc gatgccgtct gaaacgccga cggcttcaga
    24841 cggtatatcg atacacgact ttccgaaagg cacgcaggcg gggctgtgcc tgcacgaaat
    24901 tcttgaagat ttcaaattcg gacaagcagc cgccggacag gaaaccctca tcaccgacaa
    24961 gctgaaaaaa tacggatttg aagaaatatg gctgcccgcc gttgccgaaa tggcggaagc
    25021 ctgccgcaaa acgccgctga cgggggcata ctgcctgtcc gacatcccgc ccgagtgccg
    25081 ctgtcccgaa atgggcttta ccctccacac cgaagacttc ggcctcaaac ggctgcgcga
    25141 ctggtttgcc cgcgacgaca tcaggctgcc cgaagtctgc cgtgccgccg ccgaaacgct
    25201 cgacttccac accgtcaacg gctttttaaa cggctttatc gatatggtct gccaagaccc
    25261 cgacggcaat atctgcgtca tcgactacaa gtcgaaccac ctcggcacgg acgcatccgc
    25321 ctacacgcaa caggcaatgg acgaagccgt cgcgcaccat cactattacc ttcaggcact
    25381 gatttacgcc gttgcctccg cgcgctactt caaactgcgc agacaaccgc ccgccgccgt
    25441 ttccgtccgc tacctgtttt tacgcggcat agacggcaaa ggcggcggcg tttggcattg
    25501 ggacatagat gccgccgctt tggaacagat aaaataactt cccgtccctt aagacgacaa
    25561 ccgcaggaga accctatgaa tatcaaacaa ttgattaccg ccgcactcat tgcctcagcc
    25621 gcctttgccg cgcaggcagc cccgcaaaaa cccgtatccg ccgcccaaac cgcgcaacat
    25681 tcagccgttt ggatcgatgt ccgcagcgaa caggaattta gcgaaggtca tttgcacaac
    25741 gcggtcaaca tccccgtcga cccaatcgtc cgccgcatac acgaagccgc gcccgacaaa
    25801 gacacgccgg tcaacctata ctgccgcagc ggacggcgtg ccgaagccgc ccttcaagaa
    25861 ctgaaaaaag caggttatac aaatgttgcc aatcacggcg gttatgaaga cctgctcaaa
    25921 aaagggatga aatgacgcaa tgccgtctga aaggacaggc tttcagacgg catacggcgt
    25981 tccggcacgc ggcgcaacat cgggcgcggc gcgtgccgcc ccgtccgaag cctgataaac
    26041 aaaccgaaaa ggaaaaagga aatacgatga aacacatact ccccctgatt gccgcatccg
    26101 cactctgcat ttcaaccgct tcggcacatc ctgccagcga accgcaaacc caaaacgaaa
    26161 ccgctatgac cacgcatacc ctcacctcaa aatacagttt tgacgaaacc gtcagccgcc
    26221 ttgaaaccgc cataaaaagc aaagggatgg acatttttgc cgtcatcgac catcaggaag
    26281 ccgcccaccg aaacggctta acgatgcagc cggcaaaagt catcgtcttc ggcacgccca
    26341 aagccggcac gccgctgatg gtcaaagacc ccgccttcgc cctgcaactg cccctgcgcg
    26401 tcctcgttac cgaaacagac ggcaaagtac gcgccgccta taccgatacg cgcgccctca
    26461 tcgccggcag ccgcatcggt tttgacgaag tggcaaacac tttggcaaac gccgaaaaac
    26521 tgatacaaaa aaccgtaggc gaataagccc gtcgccgaca tatggcggat ggaaatcaaa
    26581 cacacacgcc cctcattccc acgaagtcgg gaatccaagc ggttgggtta ccgtgatttt
    26641 caatcattgc cgaaacggac gactccagat tcccaccctt gcggggatga cgggcgtgtg
    26701 tgtttttgcc atatcacagg taactttgcc acaaatctta tctgtcgccg gtatgccttc
    26761 ttgttttcaa tccgccacaa accaaatgcc gtctgaacct tcagacggca tttcctttca
    26821 atccctatct tcccgacaaa tatcgatggc ttcctgcaaa ctcgaaacgc cgtagatttt
    26881 caggttcgga aactctttgg cgttgcgcgg catattggct tttgggacga tggcgcgttt
    26941 aaagccgagc ttttccgcct ctttgagccg ctcttgtccg cgtgcgacgg ggcggacttc
    27001 gccgcttaag ccgatttcgc cgaaaaccac ggttttttcg ggcatggggc ggttgcggaa
    27061 gctggagagc atggcaagga tgacggcaag gtcggcggcg ggttcgccaa ttttgacgcc
    27121 gccgacggca ttgagaaaca cgtcttggtc gaaacaggca atgccgccgt ggcggttgag
    27181 gacggcgagc agcatcgcaa ggcggttttg ttccagtccg acggtaaggc gtttgggcgt
    27241 gaagccgtgc gcgtcatcga ccaatgcctg aatttcaacc aaaagcgggc ggctgccttc
    27301 ctgcgtaacc aaaacgcacg aaccgggcgt gtcgtcgcgg tagctggcaa ggaagatggc
    27361 ggacgggttg gacacacctt tcaaaccgtt ttccgtcatc gcgaacacgc ccagttcgtt
    27421 tgccgcgccg aagcggtttt tgatggcgcg tatcatgcgg tagttggaat gttggtcgcc
    27481 ctcgaaatac agcacggtat caaccatgtg ttccagcacg cgcgggccgg caatcgcgcc
    27541 gtctttggtc acgtgtccga ccagtatcat ggcgatgccc atctgtttcg ccatgcgcgt
    27601 cagttgggct gcacactcgc gcacctgcga cacggagccg ggggcggaag tgatttggtc
    27661 ggaatacatg gtttggatgg aatcgataac cacgacttcg ggctgatgct gtttcaaggc
    27721 cgtctgaatg gcttccatgc ggatttcggc aagcaggttc acgccttcgg tgggcagttc
    27781 caaacgttgc gcgcgcaagg cgacctgttg ggcggattcc tcgccggaaa cgtacagcac
    27841 tttgcggctt tgcgccattt tggcgatggt ttgcaacagc agcgtggatt tgccgatgcc
    27901 gggatcgccg ccgagcagga tgaccgcgcc atcgaccaaa ccgccaccca atacgcggtc
    27961 gagttcgccc ataccggtcg gattgcgcgg cacttcggtg gcggtcacgg cggagaggga
    28021 ctggacggtc gaggtatccg ccgcccaaga ttggaaacgg gcgtttttcg gctcgggcgc
    28081 ggctaagctt tcctgaagtg tgttccactc gccgcaatgc gggcatttgc cctgccattt
    28141 cggggaagtg ccgccgcatt cggtgcattg gtaaagggtt ttaagcgttt ttgccatcgg
    28201 ttttccctgc atctgaaaac aaaaatgccg tctgaaaata gggacggttg ggattgcgcc
    28261 aatgcggcaa cgccgccgtc atgtactatc cgtacacggc ggatattgcc gcattgcctt
    28321 atttttaccc caaccggcca tctctcggct tcagacggca tatcgggcgg tttcctgccg
    28381 gcttacccac cgtgataatg ccgcgacaat tcgtgtacgg tatcgactaa gattttggcg
    28441 tgttcgggat cggcgtgctg gttgatgccg tgtccgaggt tgaagacatg accgctgcct
    28501 tgtccgtagt cggccaggat acgtgcggct tcggtgcgga tggattcggg cgtaccgaag
    28561 agggcaaacg ggtcgaagtt gccttgcagg gcgacttgct tgccgacgcg gcggcgtgct
    28621 tcgccgatgt tgcacgtcca gtccaagccc aatgcgtctg cgccgatttc tgccatgctt
    28681 tccagccaca gcccgccgcc tttggcaaat acgataacag gcacgcggcg accttcgctt
    28741 tcgcgtttca gtccggcaac gatttggcgg atgtatttga ggctgaattc tttaaacgcc
    28801 gcgtcgctca acacgccgcc ccaagtgtcg aaaatctgca cagcctgtgc gcccgcgtcg
    28861 atttgggcgt tgaggtaggc ggtaacggct tgggcgttgg tgtcgaggat tttgtgcagc
    28921 aaatcagggc gcgaatacat catggttttg atggtgcgga attctttgct gccgccgcct
    28981 tcgaccatgt agcaggcgag cgtaaacggg ctgccggaga agccgatgag cggtacgcgg
    29041 ccgtccaatg ctttgcggat ggaagttacc gcgtcgaaaa cgtattgcag tttttccatg
    29101 tcgggaacgt gcagcttggc gatatcggct tcgtgttgca gggcgcgttt gaatttcggg
    29161 ccttcgcctt cggcaaaata cagtcccaag cccattgcgt cggggacggt caggatgtcg
    29221 gaaaacaaaa tcgccgcgtc caaatcgaag cgttccaaag gttggatggt aacttcggtc
    29281 gccaattcgg tgtttttgca caaatcgagg aagctgcccg ctttcgcgcg tgtggctttg
    29341 tattcgggca gataacgccc cgcctgacgc atcatccaaa tcggcgtgta ttcgacgggc
    29401 tgtttgagca gggcgcggag gaaagtatcg ttttttaaaa gagtcatgcg ggttttctgt
    29461 cagtctgttt gtcaagtgaa atggttttca gacggcattt caacgatgcc gtctgaaggt
    29521 ttcaacaggt ttcggcggaa gggcggtttt cctcggcaac gcttgccaaa accaagttgc
    29581 gctgcgcctc cgccttctgc ggttctccgg tttcgtcaaa aacctttgcc agaaccaaac
    29641 gcgcggaaat actcggcttt aatgcaatgc tcgcttcaag gtagcctttt gccttgcccc
    29701 aaagtttgcg gccgtaggcg agccgaccga gatacatcag cagaagcgcg ttatcgggct
    29761 gttctttcag ccaagcatcg gcaaaatcga tggctttctg ctgatcgcgt tcgcccaaaa
    29821 agcgcacgct ttcgacaaag gcttccaaaa gttcgggtcg gcggttgtgc ggataatgct
    29881 gtttgaccca tttgaccgca tcggcataca gtcccaaacg ttcgtacttt tccgcaaccg
    29941 atacgctcaa ttccccgttt ttgaggctgt cgggaatccg cttcaggcag gttttcaaag
    30001 cggcggcatc ggcagcatcc gccagctggc ggcggtatgc ccaattttga tactgtttcg
    30061 tttcggattc acccaacgca ccagccttgg aaagtttttc ggtttttgcc aacacctcaa
    30121 gcgcgtctcc tctctcgaag gcatagcgca attgcaggcg cacaagccgt gtcagtccgg
    30181 cattcatttt ggctgccgca tgaagattgg cttccaccgt ttcgtagtcc cggcgattca
    30241 gtgccgattc cgccagcagg aggtagcgtg aaagctgctg tttttcaggc agctttgcaa
    30301 tttctgcaag gtaccggtcg cgtaattcgg tatttcccat ctgtcctgcg gcgtgcgcgc
    30361 ccagcatcaa tgccaaagtc cggttgtctc cggcctcttt gttgcccaac acgcgcgagg
    30421 cttcgagttc cgccttttca aaacgccctt caaaatacgc caaacccgct ttgttcaagt
    30481 aaagcgcggc cttgcgtcct tttcgtgcca aactgaaacg ctgcatcttt tcgggaatat
    30541 tcaaaatgcc gatgacgaat ttaaacagga aataccacac cacgacggca atcagcagcc
    30601 ccagtacaaa ggcgtgcagg ttgattctga gcatggtctg tccgagtacg atatacacgt
    30661 cgccggtgta aatgcccaaa gccaatgcca gcccgacggc ggcggcaaac aggatgacga
    30721 tccagactac cgttttcatg cgcgttctcc tttagcggac ggcgaacgcc ctgccttttc
    30781 cggtacgggc tgtttttttg caggctgttc cggtttgcgt tccgaaggca gggacggtgc
    30841 ttctatggtc ttcacatccg atgcggcagc cggttcggaa gctgtgtttt cgtttgccgg
    30901 ttcggaagcc gcctgttctg cttcttgatt ttccgccgcc gtcatgcgcg taccgtcgcg
    30961 ataggcgcgg acggcattta ggctgttttt caaaccgtca tccgccgtca tgcgcacatc
    31021 caacgccttc aattccgcca gttctttcag ccacgactgc gtggcgggag acttggcatc
    31081 gaaatactgt ctgacggcgg cttcggcatt gttcaaatcg ccctgataga cttcgctgtt
    31141 gcgctgcatt aatgcagtcc gcgcatccaa aaggcggagg cgcaggtttt cacgcacaaa
    31201 atatgcctgt tcgggagaaa tcagcatggc atcgttgttt tccaaacggc ggatttcgac
    31261 cagccccttc aatgtgccga gggatttttc ccatacgttc tgccaccatg aagcagaagc
    31321 ggcttcgttc ttcacctgta cgcccggctt caatacgccg tccaaaatca gcggcagtcc
    31381 ggatacggcg gtttccagcc tgtcgaggcg caatgccgtg ccggaaatat cgacataggg
    31441 acggtttttc agttccgcca agtcgctgct gactgcctgt ttgatcggca gaagctctgc
    31501 ctgatcgaaa cgggacaggc ggctgtcgat atgctccaat acgccgactg ccgtttggat
    31561 attgccggtc aacaccagct gttgcgccgc cagattgagt atggtctcgg tttcgtccac
    31621 cagccaatcg gcgcgtcctt tggtcaactc gcgataggat ttttgcattt ccaagatttg
    31681 ttcgccgttt gctttgacgt ttccgtccaa acggtcgagc tctgattgta tggcggcttg
    31741 ccggttgagg ttgtctttca acagggcggc gttttccgac tcgcccaagg cggctttgtc
    31801 gattttttgg ttgaatgcca gctcttggtt tttcaagaca ttctgtccct ggacaaacaa
    31861 aaaaccactt gtacccaaac ccaatgccgc caataccagg gcgcagactg ccaaagcgtt
    31921 gctgccggac tgtttgacga taaaaggagc ggcaggaatg tgtacttctg tttcgttttc
    31981 tttgcgtggg gaagaggttt cagacggcat ttcttttgat gcctgtatct cgcgtacggg
    32041 ttcggatgat ttgttttcag gttcgcccac cgttttgctc cttattaatt tgaggttccg
    32101 ggaaagacca tgccgtctga aacggaaatg gaagaatggg aaagcgcggc ttccagcgta
    32161 gggacggttt cgaccgaaca cacgccttcg cgcttcaatg cctccgcaat gcgcggatga
    32221 tgggtaaagt atagcaagga tttgaagaat cgggaaaatt gcggcggaac ctgtgcaaac
    32281 agcgactgca cgagttctgc ggacgtaata taggcggcgg caatattttc ggtttggaaa
    32341 ttttgaaagt tcaaaggttt atgccgtctg aaatagactt ctgccacctc cgtccgaaaa
    32401 cctttctcct gcaaggcatt catcagaaaa tcccgcccgc cgtgtccgcg cacaaacaat
    32461 acgcgcgcac cttcgggcag actgttccaa accggcaggc gcaaaaccgc ctcgctgtcg
    32521 ttgccgtcat caggcgcgat gaccgttctg cccaaacagc gttccaatgc acggcggctg
    32581 ccctgcccta cggaaatgtg catctttatg ccgtctgaaa ggttaaggta cgggacggcg
    32641 gtttcaacgg cggtcggact gacccaaaac acggcatccg cacgggcata catttccggc
    32701 agccgtttca ggcttgcttt gtcggtttcg atttcaatcg gactcaacac ttccgcctgc
    32761 cattcggcac gacggcaggt ttccacatcg tccttcgccc tgcccgacgg tcggacaatc
    32821 agcatgatgg gtttcactgt ttctatcgcc ttatccgccc cgattaccag gcaaaatcgt
    32881 catcgtcgaa attcgcctgc gcggttttcg acgtacagtc gaaccgtgcg atttccttgc
    32941 ccctacgctc cacaaggaca ccgctgccta ctgcatgggt cacggaatcg aagcctgcat
    33001 ctacggtgta cataaattcg ccgttttgga atttcatcgt cgcccaacgg cctctgcccc
    33061 agccttgcca cctgtcggaa cgccccaaca gatcagcttt gctgttgcgt atggcaattt
    33121 ctttttttgc cgcactgccg aacgaatatt cgtaaagatt gcggttgatt ttttgaactt
    33181 ctatatattt gttgttgtcc gttttacagg gaaacaccgt atccgcccac acggtttggg
    33241 cggcagccga cagaagcaat acggcagaaa ggaacatttt tttcatcgta tttccttaat
    33301 ggttgaaact ccgccactcg gacatccgtc cttcgtggca gcagcatcag attttatttg
    33361 agaggggtgt aaccccttcc aaattagggc aacacatagg gcaacgcttt atgtgtcgtc
    33421 ctgtgtgttg aaacatcatt gcccgtatcc tgaaatagaa aagaaaatgc ccgttttacg
    33481 gtcttctttc cgacaaaatg ccgtctgaaa cggatttcag acggcatggt cgaaaattat
    33541 tgcgcggcaa gcggcttgag cagcaaggtt tcgccgcttt gcacaacttt ttcaagcagg
    33601 tcgatattgg gatgcttgcc gggatgggcg cgcgcgtcgg caacgtgttc ggcaaaccat
    33661 atcaaaccct gttctgccgc ttctttgtcc aattttccgt caaaacgttc tgccaaagcg
    33721 ttgtacaact tgagcgaacc gagcttaccc tgaacggcag gaatgtggtg aaccgttttg
    33781 ccatctgcat cctgaacgtc caaaccgccc agatggtcga ttgaaggaaa tgtggcgaga
    33841 tagtcttgaa aattcatatc aactccgaaa atcagtcagt tagcgcgtgc cgaaaatctt
    33901 gtcgcccgca tcgcccaagc cggggatgat gtagccgttc tcgttcaagt ggctgtcgag
    33961 cgcggcggtg taaatcgtaa cgtcgggatg cgcgtcgttg accgccttca caccctcggg
    34021 cgcggcaacc agcaccagtg ccttgatgtt tttgcagccc ttggctttca aaaggtcgat
    34081 ggtggcaacc atcgaaccgc ccgtcgccag catcgggtcg ataatcaggg caggacgttc
    34141 gtccatgctg tccacgaatt tctcgaagta ggaaacgggt ttcagcgttt cctcgtcgcg
    34201 ctgcagtccg actacactga ttttggcagt cggaatcagg tcgagtacgc cgtcaagcat
    34261 acccaaacct gcacgcagta tgggaacgac ggtcaatgtt ttgcccttga tgcggtcgcc
    34321 ttcaatctga ccgcaccatc cgtcgataag gtatttttcg atttcaaaat cacggcttgc
    34381 ctcgtatgcc atcaggcgcg ccagctcgat ggcaagcgtc cggaatttgt aggtgctgca
    34441 atccgcctcc ctcatcaggg ttaatttgtg gcggacgagc ggatggttga taacattaac
    34501 gttcatcgcg gtgttccagt gtgattttga gacgcgccat tatacttttt tttgatttgc
    34561 cggaaaagaa aatgccgtct gaaacacgtt cagacggcat tgccgaatcc atcaggcttt
    34621 cagcagctct tgcagctcgc ctgcctcata catttccatc aggatgtccg aaccgccgac
    34681 aaactcgccg ttcacataaa gttgggggat ggtcggccag tcgctgtatt ccttaatgcc
    34741 ttggcgcact tcgggatttt ccaatacgtt gacggtaacg taatcggtgc agcctgccgc
    34801 gttcaggatt tgcacggcgc gggaagagaa accgcattgc ggaaactgct tcgtaccttt
    34861 cataaacaat acgacgcggt gtgtcgttac tacttcttta atttggtcgt ggatggaagc
    34921 catcgcttat tccttttgat aaagttgaca aaatttgtca ggtaggacgc attatacgca
    34981 aaagcggaaa cttcccacaa tatttgccgt atatcgcgca gtatcaatgt tgtgaacgga
    35041 aatattccca ctcctcgaca atcctactgt ccggcaaatg gcacagggca tatccgccgc
    35101 cgctttcgtc tttttttact tcccgtctgc cgcctttggc aatgcagaat ttggaagcgg
    35161 aatttgccat gcctgtccga aacgtgttgg caggcagccg gcagcagcgc ggacatgcat
    35221 acggatgaat tgctttcatg gttttcctta tcgtcggtac agggtcagac gacccgcata
    35281 tcgggtactt ttttaaacag ccagtaaaag ttttccgtcg tatatgccgc aacctgttcc
    35341 aatgtttggt tccgcaattt ggcgatatgt tcggcggtat ggcgcacaaa agccggttcg
    35401 ttctgcctgc cgcgtttggg aacgggggcg aggaacggcg catcggtttc caccaaaatg
    35461 cggtcgtccg gcacatattt tgccgcctcc tgaaccaagg gtgcgttttt aaaggtaacg
    35521 attcccgaga aagaaatata aagccccaaa tccattgctg cacgggcaaa accgatgtct
    35581 tcggaaaaac agtggataac gcccgaatta acccggcatt ctttcaggat agacaaggtg
    35641 tccgccgccg catcacgcgt atggacgata acgggcagtc cggtttgatt ggctgcttcg
    35701 atgtggtctg caaagcgttt gtgctgccag gacaaatcgc ctttgcacca gtaataatcc
    35761 aaacccgtct cgccaatgcc gaccactttc ggcgaggcgg ctgcggcaac catttccgca
    35821 atagaaaatt cttcggcttc cttgctgtcg ggatgtacgc ctatggtgca atagatgtgt
    35881 tcgtgcgctt cggcgatggc aaagacttcg gagaagcttt ccctactgac gctgatggcg
    35941 agcgcctgcc ccacgccgtt tgcttccatg ttggacaaaa cttcgggcag gcgttctttc
    36001 aaaccttcaa aattgaggtg gcagtgcgaa tcgatgatat gcatggcgtt acatggtaaa
    36061 cgtaggtctg ctgccgccga ttgtgttgcc cagttcgact tcgatctggt ctttgacttt
    36121 caggcagttt tcgtgttttg tgaatgccag ccccacccct ttgggtttgg aggaagtacg
    36181 cgcaggattg atccaggcga ctttggtcgg caggaacagt ttggggaagt tgaggatttc
    36241 tacggcaagc agaatatcgt ccccgatgga aaatacgtcg ttggtctgca caaacagacc
    36301 gccgtgttcc aaaaacggca tataggagct gtacagcaga ttcatgtctt tcagctgcaa
    36361 cgacatcatt tttgccggaa tattttgtcc gtctgacata attcacccgt tctctttctt
    36421 caattccaaa taattgatga gcagatgctc gatctgcatt ttgacattta aagtatgaaa
    36481 cccgtaaggg gcaagctgtt tgagcatatc ctccgccgca aatacattgc gcggacggaa
    36541 accggatacc gtctgaagca gcctgtcttc ataagcggga taatagacgg gtttcatgtg
    36601 ttgcaggcac aatcccaaat cgaccagcca tttctgcatc cacccgacaa atacggcgag
    36661 cggaagtttt tccttatcga aaagcgcggc gtaatccaaa atcttcaaca accttggttc
    36721 tgccaaaata tccaacagtt taatccgcaa cgcacggaca ccgtccgcct catcaaacag
    36781 cggcgctccg gaatggaaag ccagacgttc ctcaggttcc gccacacccc tttcacgcag
    36841 atatgccaat gcctcttcat gggaaggcgc gggcaaaacc atcttccggc agcggctttt
    36901 aatggtcggt aaaaccttgt ccgccgcgtg gctgaccagc aaaaagacca cttgtggcgg
    36961 cggctcttcc aacactttca acaaactgtt ggcggcttgg acattcatac tttccgcagg
    37021 atgaatcaga atcacgcgca aaccgccccg taccgaagtc aggtacacat tatcgatgat
    37081 ttccctgacg gcatcgattt tgatctgcaa cagtttgcgt ccgttttcgg gttcgtccga
    37141 caagggggtg atttcgtaaa aatcgggatg gcttccctgt ccaaacagat ggcaggacat
    37201 acattcgcca cagggtttgc agcccggtgc aggggtttcg cacaacagtg ccttcgccgc
    37261 aaagcgggca aatgtagttt tccccgtatc ttttttgccg gcaaacagcc atgcattggg
    37321 acgacgctcc caatgttccg caatctgccg ccattgctca ttatgccacg gataaatcat
    37381 ttcaaaccat ccgtccggaa atccataccg ccatcagagc agttccaaat cggggctgga
    37441 actttgcgcg cggcggctgc gcaactgtac cgccaaacgc aaatcgtgtg cggaatcggc
    37501 atttttcaat gcttcttgca gggaaatatc gcctttttca tacaattggt aaaggtgttg
    37561 atcgaaggtc tgcataccca gggtagtgga ttttttcatc acttctttga tttcatggat
    37621 gttgccgttg tgaatcaact ccgaaatcag gggcgaattg agcagcacct cgactgccgc
    37681 caccctgccc ttgccgccgt ctcgcggaac gaggcgttgc gaaataaacg cctgaaggtt
    37741 gagcgacaaa tccgtcagca attgttcgcg ccgctcctcg gggaaaaagt tgatgatgcg
    37801 gtcgagtgcc tgattggtgc tgttggcgtg cagcgtcgcc atacacaaat gccccgtttc
    37861 ggcaaaggca atggcgtagt ccattgtttc gcggtcgcgg atttcgccga taaggatgac
    37921 atcgggcgcc tgacgcagcg tgtttttcaa cgccgccatc cagttttccg tatccacgcc
    37981 gacctctcgc tgggtgatga tgcagttttt gtgttcgtgg acaaactcga tcgggtcttc
    38041 gatggtgata atgtgtccga acgaattttc attgcggtag tcgataagcg aggcaagcga
    38101 agtcgatttg cccgagccgg tgccgccgac aaaaataacc agcccgcgtt ttttcagcgc
    38161 gacatccttc aagaccggcg gcaggttgag gctttcaaac ttgggaatct tgctggtaat
    38221 cgcacggaat accaacgccg tcgcgccgcg ctgtatcatc gcattgacgc ggaagcggct
    38281 ggtgtccggc aggctgatgg cgaagttgca ctcgttggtc gatgaaaatt cttccgcctg
    38341 cttcgcactc ataatcgaga aggcgatttc catacatttt tccgccgtca gcggttcgtc
    38401 cgtgatgcgg gtgattttgc cgtccagctt catcgcgggc gggaaatggg tcgtcacaaa
    38461 caggtcggaa cctttgtttt tgttcatatg gcgcagccat gcgaacagtt cttcctttgc
    38521 ggtcatattt tcatcggtaa acatcctcgt cccctaatct tcaaacaatc tgtaccttcc
    38581 cattctacca aaaacccgcc gtgccgtctg aaaccgtttc agacggcacg gacaaaccac
    38641 ctccactcaa tcctgccgtg ttagcgtgta taattcggca ttctatccga aattcacagc
    38701 aggtcagcaa tatgtctttg aaaacagtag ccgttatcgg cgcaatggaa caggaaatcg
    38761 agcttttgcg cgagatgatg gaaaatgtca aagccgtctc tttcggcaga ttttccgcct
    38821 atgaaggcga attggcggga aaacgcatgg tgcttgcatt gagcggcatc ggcaaggtca
    38881 acgccgcagt ttcaacggcc tggcttatcc atcagttcgc accggactgc gtcatcaaca
    38941 ccggcagcgc gggcggcttg agcaagggct tgaaagtcgg cgacgtggtt gtcggcacgg
    39001 aaaccgcgca ccacgatgtc gatgtaacgg cgttcggcta tgcctggggg caagtgccgc
    39061 aactgccggc aaggtttgct tcagacggca ttttgattga agcggcaaaa cgggcggcgc
    39121 ggacgtttga aggcgcggcg gtagaacaag gtctgattgt cagcggcgac cgcttcgtcc
    39181 acagcagcga aggcgtggcg gaaatccgca agcacttccc cgaagttaag gcggtagaaa
    39241 tggaagcggc ggcaatcgcc caaacctgtc atcagttgga aacgcctttc gtcatcatcc
    39301 gcgcggtttc cgattcggca gatgaaaaag cagacatcag ctttgacgag ttcttgaaaa
    39361 cggcggcggc aaattccgca aaaatggtgg cagaaatcgt caaatcttta taagaccttt
    39421 atcaaactta tgccaaaaca gcggttttcc gttagaataa cgggctattt ccacgttgca
    39481 cccgatattc ggacgcaacg ccgtttgttc agacggcatt tcccaaaccc actgtccgac
    39541 attcaaaaca actctaatga aaaatatccg aaatttctcc atcattgccc acatcgacca
    39601 cggcaaatcg acacttgccg accgcttcat ccaatattgc ggcggtttgg atttgcgcga
    39661 aatgagtacg caggtactcg attccatgga catcgaaaaa gagcgcggca ttaccatcaa
    39721 agcgcaaacc gccgcgctca actataaagc acgcgacggg caggtgtatc aactcaacct
    39781 gattgacacg ccgggacacg tcgacttctc ttacgaagtt tcccgctcct tgtccgcctg
    39841 cgaaggcgcg cttttggtcg ttgacgcgtc gcaaggcgtg gaagcgcaaa ccgtggcgaa
    39901 ctgctacacc gcgattgatt tgggcgtgga agtcgtgccg gttttgaaca aaatcgacct
    39961 gcccgccgcc gaccccgaac gcgtggaaca ggaaatcgaa gacatcatcg gcatcgatgc
    40021 cgtcggcgcg gtgcaatgtt ccgccaaaag cggcatcggc gtggaagacg ttttggaaga
    40081 aatcgttgcc aaaatccccg cacctgccgg cgacgaaaac gcgccgctgc aagcggtgat
    40141 tgtcgattcg tggtttgaca actatgtcgg cgtggttatg ctgattcgtg tgaaaaacgg
    40201 catcatcaag ctgaaagaca aagtgcgctt tatgagcacc aaggcggaaa cgcaggttga
    40261 gcagctgggc gtattcacgc cgaaatcggt tcaaaaacaa gaactcaaag ccggcgaagt
    40321 aggctttttg attaccggcg taaaagaatt gggacaggcg aaagtcggcg atacggttac
    40381 tttggttgcc aaccctgcct ccgagccgct gcccggtttc caagaagtgc aaagccaagt
    40441 attcgccgga ctttatcccg tagaaagcca cgactacgaa gccttgcgcg atgctttgga
    40501 aaaattgcag ctgaacgatg cttcgttgaa attcgagcct gaagtttccc aagcattggg
    40561 tttcggcttc cgttgcggct tcttgggcct gttgcatttg gaaatcgtgc aggaacgttt
    40621 ggaacgcgag ttcgacatgg acttgattac caccgcgccg acggtggttt atgaagtcgt
    40681 gttgaaaaac ggcgaaaaaa tcgaagtcga aaatccgtcc aaactgcccg acatcggcag
    40741 catcgaaacc atactcgagc cgattattac cgcgaccatc cttgtgccgc aggaatacgt
    40801 cggcaacgtc atgactttgt gtaaccaaaa gcgcggcgtg caggtcaata tgcagtacat
    40861 gggccgccaa gtgatgctga cttacgattt gcccatgaac gaagtggtga tggacttttt
    40921 cgacaaactc aaatccactt cgcgcggcta tgcttcgttg gattatcatt tcaaagagtt
    40981 ccaaccgtct gatttgatta agctggatat tatggtcaac ggcgaaaaag tcgatgcctt
    41041 aagcctgatt gtgcaccgtc aaagcgcggt tcaccgaggc cgcgagctgg catcgaaaat
    41101 gcgcgagctg attccgcgcc aaatgttcga cattgccgtc caagccgcca tcggcagcca
    41161 gattatcgcc cgcgaaaacg tcaaagcact gcgtaaaaac gtcttggcga aatgttacgg
    41221 cggcgatatt acgcgtaaga aaaaactgct tgaaaaacaa aaggcaggta aacgccgtat
    41281 gaaacaagtg ggcaatgtgg aaattccgca aagcgcgttc ttggcgattt tgcaggtgag
    41341 cgacaaataa ctccggcaca cagggcggcg cgtcgggaac acccctcaaa ccgccctgat
    41401 ttcaggggca cgcgcccgca aacaaatttt gcaatttgcc gccttaaaac ggttgcacgg
    41461 acaggcgggt ttttaagcat taaggaacaa caatgaacac aatgctaatg tcgggcgcgg
    41521 ctgccgcgct gcttgccggc atcatccttt atttcaaaag cgacaagaag cggcaggaaa
    41581 acggggaatg gagttccggc cttgaatacg cctatatcct gacagcggtc ggcgtgtttg
    41641 ccgctttgtc cctgtttatg agctttaccg ccgttttcct gattttcgtt gtattgtgcg
    41701 gtacggcttg gggggtatat aaataccgcc tgaagactca tcccgaaatc tcggaaagca
    41761 gccacttcgg cgattatttc ggcagtttct tccctaccgt tttggtattg ttcctcatcc
    41821 ggtcgtttat cgccgaaccg ttccaaatcc cgtccagctc gatgcgcccg ggcctgatca
    41881 agggcgattt cattttggtc ggcaaatttt cctacggcct gcgcgtaccc gttttaaaca
    41941 atatatttat tcctacaggc aaaatcgaac ggggcgatgt cgttgttttt aattatcctc
    42001 tgcagccgga gatgacctac atcaagcgta ttgtcggcat tccgggcgat gtggtcgaat
    42061 atcgggataa gattttgacg gtaaatggca aacccacttc cgacattcct gacggcacat
    42121 accgttatcc cgacgacacc gacccttccg aaatccacaa cacggatatg ttccgcagcg
    42181 gtctagacgg caaatccttc aatattctga aaaaagaagg acagcctgcc gtttccctgc
    42241 ccgtattggg caaatatacc tccgatatta tgtctgaaaa cggatattcc atagagcaaa
    42301 gcggtttgga acactgccaa tatgccgacg acggcagcgg tttcgtgtgc aaagttcccg
    42361 aaggacgcta tttcgctatg ggcgacaacc gcgacaacag tgccgattcg cgctactggg
    42421 gatttgtgga tgacaagctg gttgtcggca aggcaatgtt cattttgatg aacttcggcg
    42481 atttcggcag gtccggtacg gcaatccgtt aggccggaaa attacgccgg aacatcaaaa
    42541 atgccatccg aaccctgttt cagacggcat ttttatcatc aaacgccttg tttccctgcc
    42601 ggcagcctcg gggataatat agtggattaa atttaatcca ctatatttcc gtttgtgtgg
    42661 aaacccatcc cattgaataa agggcatttc gtccgactga cggtgtgggc ggcatatgga
    42721 aatacggaca ttattttagt tgcattgcca ttccagcgga ggcggctttt ttgattctgc
    42781 ccgccccgat gccgtctgaa agggcttcag acggcatttt ccgctccgcg ccgttgccgg
    42841 cgtgtttggt cttgccggtt ttgactgcct gccagttcaa tcgtacgcag gtttgccacg
    42901 gcagaaaatc tgatgccgga cggagtcaag gctcggactg ctgtttcagc gaaccaagat
    42961 aaattgccga aagcgtcagc gccacccctg cccactgcac gccttcaatc ggtttgccca
    43021 atccgaaata atcgatgaac agggcggcaa ccggttcgga caaaagcagc agccccgtca
    43081 gcgacagcga aagcagcgga atcgcatagg caaccatcgc ccacgcgaag cactgcatca
    43141 ccacgccgta cacaagcacc aaacccgcat ctttccaagt cgtcggataa agcgcgccgc
    43201 cgtccatcag caatgccgga acaaccaacg ataccgcgcc gcctaaactc aaaatcatca
    43261 ttgaagggaa aagcgccacc ggctcgactt catgagtctt gcggacgaac accatcgaca
    43321 gggcaagcat cagcccggac accaaaccgc tgacgaatcc ccaaaccgca ttaccgttgt
    43381 agccgaattc cgcaccggca atcatcgcca caccggcaac tgccgatatt aagcctgcct
    43441 ttttcagccc gctcaaacgc tcgccgaaaa agaaaacacc gattgccgac aagaaaaaga
    43501 tttgcaggct gttgagcagg gtggaaatac ccggcccgac cgcgtgtatg ctttcgtgcc
    43561 acaacgccaa atcgaaagcg agaaacacgc ccgccgtcag ggcatatcgg acggttttcc
    43621 tgttttttgg gaatttttgc ccgaaaaacc gtgctaaaaa ccaaaatacg aacaccgaaa
    43681 tcagcaaccg ccaaaatgcg attgcatacg aaccgacggg gacggatctg acaatcagac
    43741 tgcccagacc gaacaccacg caacccagca ccagcaatgg tgcggcatat tttttgccgt
    43801 taccgaccac aatatcccct cccttcccca tacggctgcc gcgccgacgg caagaatgcc
    43861 gccattccga aaatatgccg tctgaagccc aaagccccgg ctgcacccgg acaaacggct
    43921 gattttacgg gcaggcaggc tattttccgt tggggcaaaa aaccgtcctg ccagataaaa
    43981 cccga
//
LOCUS       DAWFJR010000006        41977 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWFJR010000006 DAWFJR010000000
VERSION     DAWFJR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 41977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41977
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.006"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(335..1081)
                     /locus_tag="WGR04_000259"
     CDS             complement(335..1081)
                     /locus_tag="WGR04_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0793899.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(1260..1931)
                     /locus_tag="WGR04_000260"
     CDS             complement(1260..1931)
                     /locus_tag="WGR04_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ0793900.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(2000..2671)
                     /gene="rpiA"
                     /locus_tag="WGR04_000261"
     CDS             complement(2000..2671)
                     /gene="rpiA"
                     /locus_tag="WGR04_000261"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ0793901.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFICIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2748..3230)
                     /locus_tag="WGR04_000262"
     CDS             complement(2748..3230)
                     /locus_tag="WGR04_000262"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ0793902.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(3262..3951)
                     /locus_tag="WGR04_000263"
     CDS             complement(3262..3951)
                     /locus_tag="WGR04_000263"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ0793903.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTLGIFER
                     HEGVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAVPVADTLKRAEGGQISATVDRSG
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIRPLLVQGDARNLKLTQPQD
                     AYIVRLLLDAV"
     gene            complement(3948..4682)
                     /gene="dnaQ"
                     /locus_tag="WGR04_000264"
     CDS             complement(3948..4682)
                     /gene="dnaQ"
                     /locus_tag="WGR04_000264"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ0793904.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVVHTETKRSGKLKVIRADENELAEHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            complement(4838..6148)
                     /locus_tag="WGR04_000265"
     CDS             complement(4838..6148)
                     /locus_tag="WGR04_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0793905.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLSETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(6138..6323)
                     /gene="ccoS"
                     /locus_tag="WGR04_000266"
     CDS             complement(6138..6323)
                     /gene="ccoS"
                     /locus_tag="WGR04_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ0793906.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6323..6622)
                     /locus_tag="WGR04_000267"
     CDS             complement(6323..6622)
                     /locus_tag="WGR04_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793907.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6973..8169
                     /locus_tag="WGR04_000268"
     CDS             6973..8169
                     /locus_tag="WGR04_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0793908.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAAVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            complement(8756..10597)
                     /locus_tag="WGR04_000269"
     CDS             complement(8756..10597)
                     /locus_tag="WGR04_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ0793909.1"
                     /translation="MRTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGVLPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVILALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFVLGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANMP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            10688..11158
                     /locus_tag="WGR04_000270"
     CDS             10688..11158
                     /locus_tag="WGR04_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ0793910.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIIEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHKIA"
     gene            complement(11229..12041)
                     /gene="hpnC"
                     /locus_tag="WGR04_000271"
     CDS             complement(11229..12041)
                     /gene="hpnC"
                     /locus_tag="WGR04_000271"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ0793911.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLQRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(12128..12610)
                     /locus_tag="WGR04_000272"
     CDS             complement(12128..12610)
                     /locus_tag="WGR04_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0793912.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            13083..13409
                     /locus_tag="WGR04_000273"
     CDS             13083..13409
                     /locus_tag="WGR04_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793913.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPAAEAPAAEAPAAEAPAT
                     EAPAAEAPAAEAAATEAPAAEAPAAEAAATEAPAAEAAATEAPAAEAAATEAPAAEAP
                     AAEAAK"
     gene            13519..14886
                     /locus_tag="WGR04_000274"
     CDS             13519..14886
                     /locus_tag="WGR04_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0793914.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGERVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            14921..15427
                     /locus_tag="WGR04_000275"
     CDS             14921..15427
                     /locus_tag="WGR04_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HEZ0793915.1"
                     /translation="MGMTATYQLVDNEKLALLKNTDTEWQELFEELEDLEDEAAVCLD
                     IDKMWDVLHFVLTGTSAENPLKGNLLSESIVGTAPISDDDGYAAYIESAQIHEIISAL
                     EGFDIKSAMEHFSMQVCKEHHIYPDIWDYEDEIYDIKEEITDYFYKIGNFYKTARHSN
                     SNVLVSIY"
     gene            15518..15748
                     /locus_tag="WGR04_000276"
     CDS             15518..15748
                     /locus_tag="WGR04_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793916.1"
                     /translation="MSKSYRKHPFSPITTAVSEKQDKRLVNRKLRRIARECLKQGKEP
                     PHHIRAVSNVYDFAKDGHFRFSASRHPHLLRK"
     gene            complement(15855..16301)
                     /gene="smpB"
                     /locus_tag="WGR04_000277"
     CDS             complement(15855..16301)
                     /gene="smpB"
                     /locus_tag="WGR04_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ0793917.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(16356..17366)
                     /gene="waaF"
                     /locus_tag="WGR04_000278"
     CDS             complement(16356..17366)
                     /gene="waaF"
                     /locus_tag="WGR04_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ0793918.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(17497..17572)
                     /locus_tag="WGR04_000279"
     tRNA            complement(17497..17572)
                     /locus_tag="WGR04_000279"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17537..17539),aa:Asn,seq:gtt)
     gene            complement(17763..18515)
                     /locus_tag="WGR04_000280"
     CDS             complement(17763..18515)
                     /locus_tag="WGR04_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ0793919.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETQVFECEVMPYPNLTEREAGQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKYMEQEITAVQKALRGRFVFREDERTQLLRQELDLYFKCRLKTFATPLEMI
                     GTAFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERDEVQTALF"
     gene            18826..19350
                     /locus_tag="WGR04_000281"
     CDS             18826..19350
                     /locus_tag="WGR04_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ0793920.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGGNASPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHRVVADIPADVHRRN
                     ATSLQLSRCANIADDQSAAISAVISLRICRIRLPPPYTAKPMPSCCNHANTPQSAASA
                     TLCGTFSSASTAAA"
     gene            complement(19439..19718)
                     /locus_tag="WGR04_000282"
                     /pseudo
     CDS             complement(19439..19718)
                     /locus_tag="WGR04_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            20335..20445
                     /locus_tag="WGR04_000283"
                     /pseudo
     CDS             20335..20445
                     /locus_tag="WGR04_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(20505..21650)
                     /gene="dapE"
                     /locus_tag="WGR04_000284"
     CDS             complement(20505..21650)
                     /gene="dapE"
                     /locus_tag="WGR04_000284"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ0793921.1"
                     /translation="MTETRSLELAKELIARPSVTPDDQNCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTQAGKLTDVARAAIAEICGIEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLNDIPKLSAVYEGILARLLAGNAV"
     gene            complement(21750..22370)
                     /locus_tag="WGR04_000285"
     CDS             complement(21750..22370)
                     /locus_tag="WGR04_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenosine tetraphosphatase"
                     /protein_id="HEZ0793922.1"
                     /translation="MLTPKSCDLFNIPFFQFAQLKKYRPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADISPIALPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     KLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGA"
     gene            complement(22404..22913)
                     /locus_tag="WGR04_000286"
     CDS             complement(22404..22913)
                     /locus_tag="WGR04_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0793923.1"
                     /translation="MNPFETQSVTFAEPIEMLYACHGKVRRFCGQVAMLPDYIAENGC
                     NQIVLQTIRQISRYFNVAAPLHHEDEEENFFPLLLQYAPQAREGVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYIPDAEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(23009..23560)
                     /gene="azu"
                     /locus_tag="WGR04_000287"
     CDS             complement(23009..23560)
                     /gene="azu"
                     /locus_tag="WGR04_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ0793924.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAVGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     DYKFACTFPGHGALMNGKVTLVD"
     gene            complement(23841..24263)
                     /locus_tag="WGR04_000288"
     CDS             complement(23841..24263)
                     /locus_tag="WGR04_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ0793925.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLILLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAAKLEKRHPELAEALANLVRRHGA"
     gene            24394..27144
                     /gene="secA"
                     /locus_tag="WGR04_000289"
     CDS             24394..27144
                     /gene="secA"
                     /locus_tag="WGR04_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ0793926.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHTLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADEALSDEEKQAQISALEDGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMKEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNTIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            27288..29060
                     /locus_tag="WGR04_000290"
     CDS             27288..29060
                     /locus_tag="WGR04_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ0793927.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPTAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEVGRILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMLTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIEALKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            29247..31175
                     /gene="rpoD"
                     /locus_tag="WGR04_000291"
     CDS             29247..31175
                     /gene="rpoD"
                     /locus_tag="WGR04_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ0793928.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            31322..32316
                     /locus_tag="WGR04_000292"
                     /pseudo
     CDS             31322..32316
                     /locus_tag="WGR04_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(32592..32795)
                     /locus_tag="WGR04_000293"
     CDS             complement(32592..32795)
                     /locus_tag="WGR04_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEZ0793929.1"
                     /translation="MELGYTPYNLRNRCKLIQAELAQIVGVKHYIQVGRWEAEPDTET
                     RRADMPLEKWRQFLDWTEKTNAV"
     gene            complement(32807..33088)
                     /locus_tag="WGR04_000294"
     CDS             complement(32807..33088)
                     /locus_tag="WGR04_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793930.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(33126..33329)
                     /locus_tag="WGR04_000295"
     CDS             complement(33126..33329)
                     /locus_tag="WGR04_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793931.1"
                     /translation="MNIIEQEMKHSSLRKVDVEIAKIIADAHKINAESVKIAQESRWY
                     PMMAATGLVTAIAAVLALIFKFA"
     gene            complement(33409..33981)
                     /locus_tag="WGR04_000296"
     CDS             complement(33409..33981)
                     /locus_tag="WGR04_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEZ0793932.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVP
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            33960..34271
                     /locus_tag="WGR04_000297"
     CDS             33960..34271
                     /locus_tag="WGR04_000297"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793933.1"
                     /translation="MTSPVSPSIRAASIFPLARSISTACRTDINRLPLRLIASTKTAE
                     INLASSGLSSVLPASLRRLRVGLMLTASSLRYSVARLMPNSCACCLRYAERAPRPIAS
                     A"
     gene            complement(34293..35168)
                     /locus_tag="WGR04_000298"
     CDS             complement(34293..35168)
                     /locus_tag="WGR04_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ0793934.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELTEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHEISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(35213..35503)
                     /locus_tag="WGR04_000299"
     CDS             complement(35213..35503)
                     /locus_tag="WGR04_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0793935.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(35515..35694)
                     /locus_tag="WGR04_000300"
     CDS             complement(35515..35694)
                     /locus_tag="WGR04_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0793936.1"
                     /translation="MYGYTVEKLKENFAQNGQTLAQWARENGFKPRDVYLVVGGQRKG
                     NYGKGHEIAKKLGLK"
     gene            35808..36185
                     /locus_tag="WGR04_000301"
     CDS             35808..36185
                     /locus_tag="WGR04_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0793937.1"
                     /translation="MISKSLFGNRLKEKRKFLGLTQAQAAEKAGIERETWGKYERGVF
                     MPSGDVLLSFLNMGIDVSSLFAAEQGIRPSENSEISKEESELLGYYRQASDNGKFVIL
                     SVAKGAEKKAAENTQVSATHKTA"
     gene            36214..36459
                     /locus_tag="WGR04_000302"
     CDS             36214..36459
                     /locus_tag="WGR04_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793938.1"
                     /translation="MFDFEQQIKWGERAEEIVKEAATQNNIEIPEPLASALAKAVKVH
                     YLSQAGVFSLVEAYADTVNPTEKEVDYQAIGKELFEK"
     gene            36749..37660
                     /locus_tag="WGR04_000303"
     CDS             36749..37660
                     /locus_tag="WGR04_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793939.1"
                     /translation="MFDLEVALRRVLSAAPTVQDTQIEQYNQLTQIMHRTLDPKDVKG
                     NSIGGLLIHIGSGTKDEHIRTMSNRPVQQDDHGGTQAPPSGYSFLRKEAFLYIVGHHV
                     IFCGHGFLSASTVASYLSLLSNKLRENNPDIVQFNIEFKAVGNCDKLSLIQQHGVKSI
                     LLDVSAYQLSRNRLYQDSRSTIAKALGKVGSVFTSELSDEELEAQSEIHINLEVLLNG
                     NSRASIEAQSLMQEQAEEIIDDETVNQGFSITTQQGEVIQPSDVKLSKSVRIGRYDEA
                     NSLLPDSAFTAISEYFLELQSRNLTEQ"
     gene            37657..38139
                     /locus_tag="WGR04_000304"
     CDS             37657..38139
                     /locus_tag="WGR04_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793940.1"
                     /translation="MKKYLPLLRFLLIACISAFLAWKGQPFVHGNEKAVDLIINVFAI
                     LAGFLIAIMTLFSDMRFDEDANWRQIQIREGVQEQRYIKHSLLFYTYLAVLVCVFIVI
                     LLAHKEEYKNGPAIFWLERSYLFLACISIFYSVFLPGNLIKSRKEEFKKLMEKKKPKI
                     "
     gene            38229..38732
                     /locus_tag="WGR04_000305"
     CDS             38229..38732
                     /locus_tag="WGR04_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ0793941.1"
                     /translation="MSDEFNQFINRVLSHEGGYVNHPQDPGGETNWGITKRTAMANGF
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VADDGIIGEISLKAINSLPENDLLLRLNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            38746..38919
                     /locus_tag="WGR04_000306"
     CDS             38746..38919
                     /locus_tag="WGR04_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793942.1"
                     /translation="MSKKSLIALMTAAMQPDFSHSDLGIRYAMPTQGCWTQAHRKSGV
                     AAAKRAAKKKRHK"
     gene            38844..39176
                     /locus_tag="WGR04_000307"
     CDS             38844..39176
                     /locus_tag="WGR04_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793943.1"
                     /translation="MLDASPPQERGSRRETRSQKKASQITAFFRWLGGLVSNPATGKI
                     SHTKLWANVAAAAMTWKFVQAADAPEWLWWAYGAMVGGYALIKRGIAVVPQLAEVKKS
                     ANQEGGRQ"
     gene            39173..39583
                     /locus_tag="WGR04_000308"
     CDS             39173..39583
                     /locus_tag="WGR04_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ0793944.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKTQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMVALKKPDALIHGKEMRQKERNSSQG"
     gene            39588..40337
                     /locus_tag="WGR04_000309"
     CDS             39588..40337
                     /locus_tag="WGR04_000309"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ0793945.1"
                     /translation="MPSVELLLEIVGLPRGTFYYQLAVQSAEDKYADLKQHIHDIYRR
                     HKGRYGYRRIAAAILHAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNI
                     LRRCFHAEKPNEKWVTDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQTRPTFDLADEI
                     LKGAPEKPGSSEKPILHSDQGWQYQMFFYSGLNLNQDKAMKPQTVQIVRQGEATPYWF
                     KFNPLYNNDRIKLKLKGLSLVQYRIQSLKAA"
     gene            complement(40656..41018)
                     /gene="ssrA"
                     /locus_tag="WGR04_000310"
     tmRNA           complement(40656..41018)
                     /gene="ssrA"
                     /locus_tag="WGR04_000310"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(41081..41686)
                     /locus_tag="WGR04_000311"
     CDS             complement(41081..41686)
                     /locus_tag="WGR04_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ0793946.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
BASE COUNT        10597 a        11525 c        10126 g         9729 t
ORIGIN      
        1 aggttcgtag gaatgacgtg gtgcaggttt ccgtatggat gggattccct cttgcgtgag
       61 gctgacagat gccgtctgaa agactttcag acggcatagc tttttctctt tgaatttata
      121 gtggattaac aaaaatcagg acaaggcggc gagccgcaga cagtacagat agtacggaac
      181 cgattcactc ggtgcttcag caccttagag aatcgttctc tttgagctaa ggcgaggcaa
      241 cgccgtactg gtttttgtta atccacgata aatttgccac aaaaaagctg cctcaaatga
      301 atacccgggc agctttttgt tgatatgact ccaatcagcg gtgttgcgga ttgtaacgtt
      361 tttccaaacg caggaatatc cagcctaaga aagtcgtcat caacagataa atcagggcga
      421 cggtgtaaag cggttcttca taaaccgaat accggcccgt aatcgtattc tgaacatacg
      481 ccaactccgc cacagcaatg accgacagca gcgagctgtc tttcaagagc gtgatgaact
      541 cgctcgccaa aggcggcagc atgcggcgca atgcctgcgg cagaatcaca tagcgcatcg
      601 cctgcggata ggtcagcccc aaagaacgcg ccgcctccat ctgtcctttg tctatagact
      661 ggatgcccgc gcggaaaatc tcacagatat acgcccccga gttggcgatc agtgccaaag
      721 aaccggcaat cagcggcccg tatccgcgac gcagcgcgat tgccgcctcg ccgctgacca
      781 aaatgccgtc tgaaggatgg acgaaaaacg gaaaccacac atacgcccaa atcacaatct
      841 gcacaaacag cggcgtaccc cggaacagcg taacatacag cagcgaaact ttacgcaacg
      901 cccacgccag cacgcgcatc ggcgcaccgg ctttttccaa gtgaatcagg cgcgccaacg
      961 ccaacaacag acccaatacc gaaccgcccg ccgtcgccac gaccgtcagc cccaaggtcg
     1021 tcagtgcgcc gtaaagaaac atccagcggt attcgtaaat aatgtcaaaa cgaaaatcca
     1081 taaaccgtcc gtatcaaaaa ccggcggaac tgccgccgtt gcaaaataat ccgccatttt
     1141 accgtaaaaa ccgccgcctg aactttttta tcgcggcaga cggcggttgc gcgtctccgc
     1201 aaaaatgcag ggcgcgcggt tttcagacgg catttgccgt tcaaagccgt gcggtgtctt
     1261 taccaaatgc ccaaccattc gcccacggca tccatccaat ccttattgcc cccgccgctc
     1321 ctgcctgccc ggcggtacgc ccacggcgct tgcggatttt tagctttcca caatcctttg
     1381 cgttcccttt ccgcctgaat ttgagcgtcg gcataatcgg caaaatccgc cttatcctgc
     1441 tgttctttag cataactttt ataatgccac gccgccccgt cctgcacctg catcaggttc
     1501 aaatcggttt tgccgacaga aacctgcgcc acttcgcgct ggtagcggtc ggtgtcgaac
     1561 acgcggacgc tgactttcct gccttccgcc gccgcacgca ggttgtcgcg cgaacgcgtg
     1621 ccgtaagcct gtttcatctc cggcgcgtcg atatacgcca tccggatttt gtgtttcgcg
     1681 ccgtcgccgt cgataacgtg aagggtgtcg ccgtcataga ctttggacac cgtgcctgtg
     1741 tagcggtggc cggatttcgc cgatgctcgg cggcgggcgg gcgcgtcgga acccgcgtcc
     1801 cctgccgcgc cgagtacgtc gagtacggca accgccgtcc gcaccgcctc gctgccgtac
     1861 cccgtataac ccaacgcacc caaaagcgac aaggcgacgg gaagccattt catgattttt
     1921 ttaatctgca tatttttcaa atgccgatgc cgtctgaaca tatcggaatc ggatttcaga
     1981 cggcatctta acgtcaggat tacccttggc agggatagat gactttcgca ccctcttccg
     2041 tccccaaaat caacacatcg gcggcatcgc gggcgaatat gccgttttcg agcacgccgg
     2101 tgattttgtt gatttcgtct tccatcgtca gcggctgatc gatattcaag ccgtggacat
     2161 cgacgatttg gttgccgtaa aacgtggtgt agccgatacg cagttcgggc tgtccgccca
     2221 tagcgagcag tttgcgcgaa acaagggaac gcgcgctttc gacgacttcc acgggcagag
     2281 ggaatttgcc caaacgtgaa acatatttgc tttcatccgc aatgcagatg aatttttctg
     2341 atgcgctggc gacgattttt tcgttgaggt gcgcgccgcc gccgccctta atcatttgca
     2401 gggcgtggtt cacttcatcc gcaccgtcga tatagaccgc caaacccgat acttcgttca
     2461 gggaaacgac gggaatgtcg tactgggcaa gcagttcgct ggattttttg gaggttgaaa
     2521 ccgcgccttt gatttttttg ccgctcttgc ccaaggcttc gatgaaaaag ttgatggtcg
     2581 agccggtacc gatgccgatg tattcgtttt cgggtacgaa ttcgactgct ttttcggcag
     2641 cgatgcgctt gagttcgtct tgtgtcgtca tatttttgtc ctttgggaaa ccgtatcaac
     2701 aaacagccgc catcttaaca tttatttgca cgtcctgccc gccgcgttca aatccgtacc
     2761 agcaataccg ccgcctgcgc ctctatgcct tccatgcgcc cgagataacc gagtttttcg
     2821 ttggttttac ctttgatgtt gacacacgaa atgtctatgc ccaaatcggc ggcgatgttg
     2881 gcacgcattt gcggaatatg cggcgcgagt ttgggtttct gtgcaatcac ggtcgtatcg
     2941 acattgaccg cctgccaacc ctgcgcctga acgctttgat acgccgcacg caaaaggacg
     3001 cggctgtccg catctttgaa ctctgcggcg gtgtcgggga aatggctgcc gatatcgccc
     3061 aaacctgccg cgccaagcag cgcgtcggta acggcgtgca gcagcgcatc ggcatcggag
     3121 tgtccgagca gccctttttc aaatgggatt tcaactccgc caagtatcag ctttctgcct
     3181 tcggtcagtt ggtggacatc gtagccctgt ccgatacgga tgttcgtcat cgtttgtgtt
     3241 cctgatggtt tgaattgaag ttcagacggc atcgagcagc agcctgacga tgtatgcgtc
     3301 ctgcggctgc gtcagtttca aattgcgcgc gtcgccctgc accagcaaag ggcggatgcc
     3361 caatttttcc acggcggacg cttcatcggt aatgccgtcc aagttttccg cagccaatgc
     3421 gcggtgcagc agcccggcgc ggaaaagctg cggcgtttgc gcctgccaaa gccccgaacg
     3481 gtcgacagtt gcactgattt gtccgccttc tgcgcgtttg agcgtatcgg caacgggaac
     3541 tgccaaaatt ccgccttcgg cagcgttgcc cgcctgttct atcaaccgcg tcagagcttc
     3601 agacggcagg cagcatcggg cggcatcgtg taccaaaata ttgtcggttt ccgccgccaa
     3661 accggtttcc aacaattttg ccacaccgtt tcggacggtt tcggcgcggg tctgtccgcc
     3721 gtttttccac acccgaacct gtggaaatgc cgtctgaacc ttatcggcaa acgtgtcttc
     3781 gggcgagacg acaacgacgg tcaaatcgac gccctcatgc cgttcaaaaa tcccaagcgt
     3841 atgttctaaa acggttttgc ttccgatttc gacatattgc ttgggtttgt ccgcaccgaa
     3901 acgcgccccg atgccggcgg cgggaatcag cgcgatattt ttgcgcttca tgcgtccgtc
     3961 ccgccgtttt cagacggcac ggcttccttg cgccagatac aggcttcgcc caagccgtcc
     4021 aaatattgcc cgtgttccgc caactcgttt tcgtccgccc tgatgacttt cagtttgccg
     4081 ctgcgtttgg tttcggtatg caccacgggt ttggtttcca ttttttcctc tgcggccgca
     4141 cccatcaggt cgaactgccg ccgcgtcata gcaagataga cttcgcccaa aagttcgcag
     4201 tcgatcaatg cgccgtgcag gacgcgcttg ctgcggtcga cggaaaaacg gttgcacaag
     4261 gcatccaggc tggctttctg cccggggaac atttcgcgcg ccatcgccag ggtatcggta
     4321 acggtacagc cgagttcctc aacggtcggc aaccccatcc ggcggaactc catattgagg
     4381 aagcccacgt cgaatttggc attgtggata atcagttccg caccgcgcag gaaatcggca
     4441 atctgcctgc cgacctctgc aaacggcggc gcgtttttcc cttccaaaac ctgtatcgtc
     4501 aagccgtgga cgcgtgccgc ctcttcgggc atatcgcgct cggggtggac atagaggtgc
     4561 aggtttttgt cggtcatttg gcggttgacc atttccaaac cggcaaactc gaccaagcgg
     4621 tcgccgccgt cggcatacag accggtggtt tcggtatcga ggatgatttg gcgtgtcgtc
     4681 atatcggtgt ctttcttcta tcttcgtaaa ttgcttattt tttaagcaat gtatttttct
     4741 gttttcattt caatgcacaa acccacttat tcacagtgtg ttcacaacat tgggcaggcg
     4801 gattgtgtat tttggggaca attttttcag acggcattca aggttttttc ctgattgccg
     4861 ccgcgcctaa aaaccgcctt tcgcgcttaa tcaaaaatac cgacaacgga atattgccca
     4921 aagcgacaat cagatacaac aaggaaatgc tgtcaaacaa aaacagcaac accgcgctca
     4981 aaacggcggc ggaaaccata aaaataccgt taacgatatt gttggcggca acggcgcggg
     5041 cgcggaaagt ctcgctactg gcggtttgca gccaagtata gagcggaacg gagaaaaatc
     5101 cgccgaaaaa gccgatcagc gtcatcaccg ccatcacggg atatgcccat ccttgcgata
     5161 aaaaccaaaa aatgccgttc agcccttcaa aacggtgtcc gtgcgtcagc cacaccaaaa
     5221 ccaggccgca aaccgtcaaa cccaacgcac caaccgttac ccaagccaac atcagccgtt
     5281 ccctgctgaa cttggcacac agtaccgaac cggcggcaat accgatggaa aacagggcaa
     5341 gcatcaggtt gaaaacattg tcgttgccgc ccagatggat ttgggtaaag gtcggcagtt
     5401 gcgtggtata aaccgcgccg acaaaccaaa accacgaaat accgataatg gcggtaaaaa
     5461 cgggcttgtg ccgcaccgtt tcactcagca gggattttgt gccacggaca atattccact
     5521 caatttgtgt atcggcagcc ttggcgggta cggacggcat aaacaggctg ccgaccgtgc
     5581 ctccgacggc gaccagcaaa accagtatcc cgacaatata aggcggtaca cctgccaccg
     5641 ccgttcccaa aatctgaccg aacaggatgg cgacaaacgt acccgattca atcaggctgt
     5701 tgcccatcat caactctttg tcgtcgagat aatcgggcag gatggcgtat ttcagcggcc
     5761 cgaacagcgt cgattgcgcg cccatgcaaa acagacacgc caaaagcagc ggggcagacc
     5821 ggatataaaa cccgtatgcc gccaccgcca taatgatcat ttccagcacc ttgacccaac
     5881 gcgccaaaac ggccttgtcg aatttgttac ccaactgccc cgacagcgag gaaaacagga
     5941 aatacggcaa aataaacagc aacgcgccca agttcaacat ctgtccggca ggcaggaagc
     6001 cgttttgccc caaaccgtaa aacccaatca tcacaaacag cgcggttttg aacacattgt
     6061 cgttgaacgc gccgagaaac tgcgtagcga aaagaggtgc gaaacggcgg cttttaacca
     6121 gtcccaaacc gcctttttta gcgtacatcg ttttccctct ctttttcaat cagtttactt
     6181 gtcgaatcat catccatcag gatgcggtgc gccggccctt ccaagtcgtc aaactgcccg
     6241 tttttgcccg accaccaaaa aaaccagccg atgacaaacg ccaaaataat gctgatgggc
     6301 accaatataa acatgctttc catcacatat tccctgtcaa atcgttcaaa acaaaagtct
     6361 gccccgacac ggtcagatat tcgttacgca aagttccgac aggtgcttcg tcaaaaaaca
     6421 gctcgatacg gtctttgacc acgcgccaat attgggggat ttccgtctga ccgaacggcg
     6481 acaggacatg attttccatt ccgccttcaa gtttgacggc aaaacgcccg ctttgcggcc
     6541 gtgcttccga ttcgtcgtcg gcaagcagga tgaaaaagcc tatatgccgt cccgattggt
     6601 catgaatact gaaataatgc ataaatttcc cacccgcctt ttttcagacg acaccaacta
     6661 aaaacagggc gaatgtacca gtttggacgg gaagaatgca aagaaattct ccctccccca
     6721 gccgaaaaca ccggcaaacc gcatatcccc cttttttccg tcaaaatacc cgacttccgc
     6781 cattttcacg caaacgcccg attaagccaa gcaattgcaa agattttttg ttagaataac
     6841 ctgcttcttt tatcaaccgt ttcagacggc cccactactt tcccgcccag gaaggcaaaa
     6901 cggattcggc acgaatccgg ttagtatccg tgtccgattc caatgccgtc tgaaactttc
     6961 cggagtaaga aaatgtccca aaaattgatc ttggttttga actgcggcag ctcgtccctc
     7021 aaaggcgcgg tcctggataa cggcagcggc gaagtcctgc tcagctgcct tgccgaaaaa
     7081 ctcaacctgc ccgatgccta catcacattc aaagtaaacg gcgaaaaaca caaagtcgat
     7141 ctgtccgcac atcccgacca caccggcgcg gtcgaagccc tgatggaaga actcaaagcc
     7201 cacggcctcg acagccgcat cggcgccatc ggccaccgcg tcgtcagcgg cggcgaactg
     7261 tacagcgaat ccatcctcgt tgacgacgaa gtcattgccg gcatcgaaaa atgcatcccg
     7321 ctcgcccccc tgcacaaccc cgcccacctc ttgggcctgc gtgccgcgca aagcattttc
     7381 aaaggcctgc ccaacgtcgt cgtattcgat acctccttcc accaaaccat gcccgaagtc
     7441 gcctacaaat acgccgttcc gcaggagttg tatgaaaaat acggcctgcg ccgttacggc
     7501 gcgcacggta ccagctaccg cttcgtcgcc gacgaaaccg cgcgcttcct cggcaaagac
     7561 aaaaaagacc tgcgtatggt cattgcccac ttgggcaacg gcgcgtccat caccgccgtc
     7621 gccaacggcg aatcgcgcga caccagtatg ggcctgaccc cgctggaagg gctggtaatg
     7681 ggtacgcgca gcggcgacat cgatccttcc gtattcggct tcctcgccga aaacgccaat
     7741 atgaccatcg cccaaatcac tgaaatgctg aataaaaaat ccggtctgct cggcatttcc
     7801 ggcctgtcca acgactgccg caccattgaa gaagaagccg ccaaggggca taaaggcgcg
     7861 aaattggcct tggatatgtt tatctaccgc cttgccaaat acatcggcag tatggcggtt
     7921 gccgcaggcg gtttggacgc actggtcttt accggcggca tcggcgaaaa ctccgacatc
     7981 atccgcgaac gcgtgatcgg ctacttgggc ttcctcggtc tgaacatcga ccaagaagcc
     8041 aacctgaaag cccgcttcgg caacgccgcc gtgattacca ctgccgacag caaagccgtt
     8101 gccgtggtca ttccgactaa cgaagagctg atgattgccc acgacactgc ccgtttgagc
     8161 ggtctgtaag gttttatccg cacacgaact gcctccggaa atggaggcag tttttttatc
     8221 cggctttcca tgcttaaaca gcactgcctc ttttcagaca ttgacggttg cagccgctta
     8281 cctgaacctt atagtggatt aaatttaaat cagtacggcg ttgcctcgcc ttgccgtact
     8341 atctgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatatccga
     8401 ctttccatac ttaaacagca ctgcctcttt tcagacattg acggttgcag ccgcccacct
     8461 gaaccttaat gattgactat tttttaatca tgttattatt ttccataaaa tacatgacat
     8521 taagatgttt ttccacaaaa gatacacaca ccggcaaaca ccggctgtgt ttatcttttc
     8581 ttatgcctat tttttaatca tcgtattttt atcttttaat ttcaatacgc aaactaactt
     8641 atacacacgg ttttcacatc tttggactgc ttccgtgtgt atagtggata ttgccgtttt
     8701 cctttctgac aaaaatgccg tctgagaact tcagacggca tttgaaacat cggaatcagc
     8761 ggttttgttc ataccactcg ataaacttgt ctgctttgac aaaacccagc agcggctcgc
     8821 tgcggctgcc gtcggagcgg acgacaaaca cgcccggcgg cccgaacaga ccgtattctt
     8881 tcaacaacgc ctgatgttcg ggcatgttgg cggttacatc gatttggaaa aagcgttcca
     8941 tatcgactgc ctgatgcact tccggctgat tgagcgtgta agccgccatt tctttgcagg
     9001 aaatgcacca gtcggcataa aaatccaaaa cgacgggttt gtcgggatgt tctttcaacg
     9061 ccgtatccat cgctgccttc agcgcggcag tatcggcaaa catttttccg tgttccgaag
     9121 atttgcctgc ttcagccggc ggattgaggg tcaggaaatg gtgcagcgcg gtcgttttgc
     9181 cgtttgcgcc ctgccagccg aaccacgcgc cgcctatcag caatatgccg cccaatacga
     9241 atgccacagc tttcggacgg cgtttctgcc tgcgtccgtt gaccagcagc atacaggcag
     9301 gaaccagcat cagcagcgtg tacagcgcga cgacgagata atagggcaag tgcggcgtgg
     9361 cgaggtaaac ggcgacggca agcaggataa agccgaaagc gtatttgacg gcattcatcc
     9421 aatcgcctgc cttaggcagg atatgcccgc cgaacgtgcc gatggcaatc agcggaacgc
     9481 cggtgcccaa cgccaaagtg taaagtgcca aaccgcctaa aaccgcatcg cccgtctgac
     9541 cgatgtagcc caaagcaaat gccagcggcg gggcgacgca cggcccgaca atcagcgcgg
     9601 acaatatgcc cataataaag acggaaacga ttttaccgcc tgaaagcctg ctgctttgat
     9661 tctgaaaata cgactgcacg gcgttgggaa gctggatgtt gaacagcccg aacatagaca
     9721 gtgccaagat gaccattaaa gccgatgccg ccaacaccac ccaagcctgc tgcaaccata
     9781 cggtcagcag tgcgcccgtc agtccggcaa caatgccgac cagcgtataa gtcagagcca
     9841 aaccctgaac ataaacgacg gacagcacaa acgcccgcgc cttgcccgcc tttttgtcgc
     9901 cgaccacaat actggaaaca atcggcaaca ggggatacat acaggcggta aaactcagac
     9961 ccaaaccggc gagaaaaaac gccaaaagat tggcgttgag cgtatcccaa gacagcttga
    10021 aacggctgtc gccgccctca tcccccttcg ggggcggcag caccccgctg ccgttttgag
    10081 aggaaggctg caaaaagcgg tctttggcgg atgccggttc gtcggtttgc ggatggtaag
    10141 tgccgttgcc ggaaatatca aactcggtat ccacgggcgg atagcacacg ccggcttcgg
    10201 cacagccctg ataggtcaaa accaatttat acggttcgcc gacagccttt gcataaggaa
    10261 aggcaacctg cgcttcgtga tggtaaaccg tctgcctgcc gaaaaactcg tcttccttct
    10321 cttcaccctt gctgaaagaa ggctgtccca acaaatccgc cggatcggtc ttgccgacga
    10381 ttttcgcctg atacatatag tatccgtcgg caatcctgaa acggacgttc acaccgtcgt
    10441 cggcaacggc aagctccggc acgaatgcct tttccggcgg cagcagatcg ttcgcatcca
    10501 gcgcgaaagc tcgtccgcac aacatcaaaa atacggcgaa caggcaaatc agttttttca
    10561 taatcgaatc cgtttcagac aaataatttg tccgcattat aaatggtaag gttgacggtg
    10621 ggatttaatt tatgtaaaac ccgccattat ccgaacctat ttccataaac atcttaccga
    10681 acccgccatg tacgatgtca atacccacga tgtccgccgc tttttcgccc gcgtgtggca
    10741 gcagcggctc aatccgctgc aactgagcgc actggaacag aaagccctcc gcattatcga
    10801 agcccatccc gaataccacc gttatctcga acgcatcgaa gaccatctgg acaccgactg
    10861 gctgcccgaa aacggcgaaa gcaacccctt cctgcatatg tcgctgcatt tgtccgtcca
    10921 agaacaggcg ggcatagacc agccgcacgg catacgcgca atccacgaca ccctgtgcgc
    10981 caaacgcggc tggctggaag ccgaacacga aatgatggag gcactggcgg aaacactgtg
    11041 gacggcgcaa cgctacggca ccggtttgga tgtcaatttc tacatgaccc gactgcgcaa
    11101 actcatcggc ttgggtgcag aagatcaagc caggttgaac ccgcataaaa tcgcctgacc
    11161 ataccaaccg cctgcaaaat gccgtctgaa gcggaacaac ccctttcaga cggcatgcat
    11221 ttcccccatc atttccacaa cgcacgtttc agcataatca accaatcctt cttatccaaa
    11281 acggggcgtt gtgcaaacac atcgtatcgg cacgcgtcca gtttctgcaa aatcaactgc
    11341 gcccctaaca caatcatacg gagttccaaa ccgatacgcc ctttcaattc gcgcgccaaa
    11401 ggcgaacccg ccttcagcat acgaaatgcg cgccggcact catacgccat cagccgctga
    11461 aacgccgcat ccgccctacc ctccgcaatc tgtccttcgg aaacaccgaa tttcaacaaa
    11521 tcgtcctgcg gaatataaac cctgcccttt tgccaatcca cagccacatc ctgccaaaaa
    11581 ttcaccagtt gcaaagccgt acagatgccg tcgctttgcg ccacgcacac cgcatccgtt
    11641 ttcccgtaca aagccagcat aatgcgtccg acagggttgg cggaacgccg acaataatcg
    11701 gccagctcgc cgaaatgcgc gtaccgcgtt ttaaccacat cctgagaaaa tgcagaaagc
    11761 aaatcataaa acggctgcaa atccaaaccg aacggcacaa ccgcctcggc atccaatcgt
    11821 gcaatcaaag gatgcgccga ccggccgccc gatgccaaca cgtccaactc gcgctgcaaa
    11881 ccctccaacc ccgccaacct ggcttcagac ggcatactgc cctcgtccgc catatcgtcc
    11941 gccgtccgtg caaacgcgta caccgcgtga accggcttcc tcaacctgcg cggcaaaatc
    12001 agcgaaccga cgggaaaatt ctcataatgc ccaaccgaca taccttctcc atccatcaaa
    12061 caaaatgccg tctgaaacgg aacaaaccct tttcagacgg catcagatac ctccaagctg
    12121 ccggcaatca gtggtggtga tgaccgtgcg ggccgtggac atggccgtgt gcgatttcct
    12181 catcggatgc atcgcgcacg ctttcaaccg tagccttaaa gcggattttc atgcctgcca
    12241 aaggatggtt gccgtccacc accgccttgc cgtcggcaac atcggttaca cgatagacga
    12301 caacatcgcc ggtttcagga tcgtcggctt caaacatcat gccgacttcg acttcaacag
    12361 ggaacacgcc cgcatcttcg atacggacca actccggatc ctgctcgccg aacgcatcgt
    12421 cgggcgacag cgccacatcg accgtatcgc cggcatcctt accgtgcaac gcctcttcca
    12481 ccaaagggaa aatgccgtcg taaccgccgt gcagatacgc aatcggttct tcggttttgt
    12541 ccaaaagctg attgttggca tcatacatct cataatgcag cgaaaccacg gaatttttca
    12601 cgatagccat atttgtcctt tcaggaacag cagattgatt acaggcgcat tctaacacaa
    12661 ccgccgcgcc ggccgattac cgttaacctg ttcataaact gtacagcaca tatttcaatg
    12721 taaatttttg ttattttatt gcggtgtaac ttttttacaa cattcttaaa accattccga
    12781 cctgtctgcc gactttccca atccgcctta ataaatcata caagatactg aaattatatt
    12841 aatctctata atatttatcc ctatcgaatt tttaacagca aaaccgtttt acaggattta
    12901 tcaatccgcc cgccagaaaa cttttcattc aaaccttttt cccatctgta cgacattgca
    12961 atcccttatt ccatagtgca taattacgca aattcagcga tgaatttcca acccggtttg
    13021 tagtatggtc gataaagacc tatttgtttc aataatttaa attggttcta aaggttacta
    13081 aaatgaaaaa atccctgttt gccgctgctt tgttgtcttt ggttctggca gcctgcggcg
    13141 gtgaaaaagc cgctgaagct cccgctgctg aagctcccgc tgctgaagca cctgccgctg
    13201 aagctcccgc taccgaagca cctgccgccg aagctcccgc tgctgaagct gccgctactg
    13261 aagcacctgc cgccgaagct cccgctgctg aagctgccgc taccgaagct cccgctgctg
    13321 aagctgccgc taccgaagca cctgccgctg aagctgccgc taccgaagca cctgccgctg
    13381 aagctcctgc tgccgaagct gcaaaataag cattttccgc ttgcaaaaaa gcaggatacg
    13441 ttcagtatcc tgcttttttg atttttcaga cggcatcaga ttcccttcct caatcttctc
    13501 cctacccttc cgacaaacat gcctgacctt cataccgaat tttcccgact cctgccggca
    13561 gatgaaattg ccgaactttc tccgacgctt ttaaaagacc agcgcaaccg ctttacgtct
    13621 gcaccagaca tcattttgca gccgcgcagc gttgaaagcg tgcaaaccat tatgcgtttt
    13681 tgttacgaac accgtattcc ggttacgccg caaggcggca ataccggttt gtgcggcgcg
    13741 gcagtatcgg aaaacggcgt attgctgaac ctttccaaac tcaaccgcat ccgcagcatc
    13801 aatttgtcag acaactgcat aaccgtcgaa gcaggttccg tactccaaac cgtccaacag
    13861 gcagccgaag cctcaaacag gctgttccca ctcagtctcg ccagcgaagg ctcgtgccaa
    13921 atcggcggca acatcgcctg caatgccgga ggtttgaacg tattgcgtta cggcacgatg
    13981 cgcgacctgg ttatcggttt ggaagtcgtc ctccccaacg gcgaacgggt ttcccatctc
    14041 catcccctgc ataaaaacac caccggctac gacctgcgcc atctgtttat cggcagcgaa
    14101 ggcacactgg gcatcatcac tgccgccaca ctcaagcttt ttgcccgtcc ctcggacaag
    14161 gcaacggcat gggtcggcat acccgacatc gaatccgccg tccgcctgct gaccgaaacc
    14221 caagctcact ttgccgaacg cctatgcagt tttgagctga tcggccgttt tgccgccgag
    14281 ttgtcttccg aattcagcaa attccccctg ccgacacatt cagaatggca tattttactt
    14341 gagttgaccg actcattacc cgacagcaat cttgatgatc ggcttgtcga atttctttat
    14401 aaaaaaggat ttaccgacag cgtattggca caaagcgaac aagaacgtat ccatatgtgg
    14461 gcattgcgcg aaaacatctc cgcatcgcaa cgcaaactgg gcaccagcat caaacacgat
    14521 attgccattc ctatcggacg cgttgccgac tttgtccgcc ggtgcgccaa agatttggaa
    14581 cagaatttta aaggcataca aatcgtctgc ttcggacatc ttggcgacgg cagcctgcac
    14641 tacaatactt tcctgcccga aatcctcagc aatgaagtct atcgttacga aaacgacatc
    14701 aacgacgcag tttatcgcaa cgttcttgcc tgcaacggca cgattgccgc cgaacacggc
    14761 ataggtatca tcaaaaaaca gtggctggac aaagtacgca cgcctgccga aatcgccctg
    14821 atgaaaagca tcaaacaaca ccttgatcca tataacatta tgaatccggg caaactgctt
    14881 ccgtaaataa ctgattcaaa atgaaattat aggaaatatc atgggaatga cagcaaccta
    14941 tcaactggtc gataatgaaa aactggcttt gcttaaaaac accgataccg aatggcaaga
    15001 attgtttgaa gaactggaag atttggagga tgaggctgcc gtctgcctcg acatcgataa
    15061 aatgtgggat gttttgcatt ttgtactgac cggaacaagt gcagaaaatc ccttaaaggg
    15121 caatctgtta tccgaatcaa tcgtcggaac ggcacccatt tcggacgacg acggatacgc
    15181 cgcttatatc gaatccgccc aaatccatga aatcatctca gctttggaag gcttcgacat
    15241 aaaaagcgca atggagcatt tcagtatgca ggtgtgcaaa gagcatcata tctaccccga
    15301 catttgggat tatgaagatg aaatttatga tataaaagaa gaaattaccg actactttta
    15361 caaaatcggc aatttttaca aaaccgcccg gcattcaaat agcaatgtac tggtcagcat
    15421 ttactaaata tatttcaaac atcacggaaa ctttaatgaa gacaaagtac ggaatcgcac
    15481 tcaaggaaac ctaggtattt caacctggga gaccatcatg agtaaaagtt acagaaaaca
    15541 ccccttttcc cctataacca cggcagtcag cgaaaaacag gacaaacgcc ttgtcaaccg
    15601 gaaactacgg agaatagcgc gcgaatgtct gaaacaagga aaagaaccac cccaccatat
    15661 cagggcagtc agcaacgttt acgatttcgc caaagacggg catttccgtt tttccgcttc
    15721 ccgtcatccg catcttttac gcaaatagaa aacagggcaa ccgccctgtt tttccgtttc
    15781 gtatttcaac accattgttc aaacaaccgt cagacggcat tgttcaaacg ccgtctgaaa
    15841 ccgcatcccc cccatcagcg cgtatgctta atcaaccgct gtttctcgcg tttccaatcc
    15901 gcctctttca tactctgacg tttgtcgtgc tgtttcttac cttttgccaa accgatttcc
    15961 atcttgattt ttccgcgtga aaaatgcaaa tccagtggca cgatggtgta gccggcacgt
    16021 tccgtttttc cgattaattt gttgatttcc gactggttca acaagagctt gcgcggacgt
    16081 acggcatctg gtttaatgtg tgtcgaagcc gtaggcaaag ccgtaatatg gcagccgacc
    16141 agataaaacg cgtctttttt ccaatagata taactctctt taagctgtac gcgcgcggcg
    16201 cggattgctt tgacttccca gccttccaag accaaaccgg cttcaatccg gtcttcaatg
    16261 aaaaaatcgt gaaatgcttt tttattgttc gcaatagcca taaacatcct atcaatatcc
    16321 gccgtcagac ggcataaacc cgaaaacaga acccatcata ccgcctcttc aaccgcctgc
    16381 acaatcttct cgggatacag cctgttgagg cagtcggtat gccccagcgg acattcccgc
    16441 ttaaaacagg gcgaacattc caagtgcagg ctgacgattt tcgccctgtc gctcaaaggc
    16501 ggcgtatgcg tcgggctgga agaaccgtaa accgccacca ccttcctgcc caatgccgcc
    16561 gccaagtgca tcagcccgct gtcgttgcac acaaccgtgt ccgccaacga cagcaaatcc
    16621 attgcctgcg acaaatcggt tttgccgcac aaattgacac acataccgtc tgaaaggcag
    16681 ttgatttcct cggcaatttc atcatctttt tgcgaaccga acagccaaac ctgccaaccc
    16741 gccgccagat aatgtttgcc caactcggca aaatgccttg tcggccaacg ctttgccggc
    16801 ccgaattccg cgcccgggca aaatgccaaa acaggctttc caagattcaa accgaaagtt
    16861 tcgacagaaa tttcccgccg ccgttcatca atggaaaact cggggaatcc cgaatgcccg
    16921 tcaaaatctt cctgactcgg atgcgcgaga gccgtatatc tgtccaccat caaaggcaga
    16981 cgttccttat ccagcctgcg tatatcgttc aacagaaaat aacggctttc gccgacataa
    17041 cccgttcttt ttccgatacc cgttgccagc gcgatgattg ccgatttcaa agaacccggc
    17101 aacacgataa cctgatcgta tccgcgccgc cccaaatccc tgccgacccg ccagcggcgt
    17161 ttcaactcca acgcaccgtg tccgaacgga ttttcaagaa tctcattcac ttcaggcata
    17221 cgctcaaaca ccgccatcga ccacttcggt gcgaacacat caatcgtgca accggggtga
    17281 agttccttca aacggcggaa caagggctgg gtcatcacgc agtcgcctat ccaactgggg
    17341 gaaataatca ggattttgat ggacataaca agaaaccgaa atcagacagg cggaatttta
    17401 ccgcgaaacc gttggaaaac ctatcttgcc gcattccgaa cgtcggacgt gcaaatatga
    17461 aaaagcccga acattcaagt tcgggcttca aaattctggc tccccgacct gggctcgaac
    17521 cagggacctg cggattaaca gtccgtcgct ctaccgactg agctatcggg gaatggggcg
    17581 tattatagcg tccggaaaaa atgtgtcaat ccttaatttt ggaaaaatag gcgacaaaac
    17641 gacaagcata tgaatcagaa agacattaag accgatgcct taaaaggatt gccgttgtat
    17701 gaatttccac agccgtcatc acaccatatt taagcccgat gagccgttct gccctccccc
    17761 gcttaaaaca atgccgtctg aacttcgtcg cgttccaaag ccagtaaaaa ctgtttgcgg
    17821 ttcaacccgc ctgcgtaacc ggtcagtttg ccgtcgctgc cgatgacgcg gtggcaggga
    17881 atcaagatag acaccttgtt ctgcccgttg gcggcggcaa cggcgcggac ggctttgggg
    17941 ttgcccaaac gctgcgcctg ctccttataa ctgcgcgttt cgccgtaagg aatcgccaaa
    18001 agtgcgtccc acgcctgttt ttgaaacgcc gtaccaatca tttccaaggg cgtggcaaag
    18061 gttttcagac ggcatttgaa gtataagtcc aattcctgcc tcagaagttg cgttcgctca
    18121 tcttcccgaa acacaaaccg cccgcgcaaa gctttttgga cggcagtaat ttcctgctcc
    18181 atatatttct gtccgacaaa ctccagcaga cacaatccct tatttccaaa caccgccaac
    18241 atctcgccca aaggcgtggc aatggcggca cacaccagct cgttcaaact gtcgggataa
    18301 cgcgcttcca acagacggat agctcggcgg atacggacat attcttcagg cgcgcagccg
    18361 atattgtccc aaaaatcccg ctcgaactgc ccggcttcgc gttccgtcag attgggatac
    18421 ggcataactt cgcactcaaa aacttgggtt tcgaggcaat ggcggatttc atcccatttt
    18481 gacggcagat tgtttaagga aggcagggta atcattcgtt tgctccgtat ctctatcatg
    18541 gatttgacgg caaaattccc aattttcgcc attcccgcac gctgatgcag gaacgggcta
    18601 cgacgtaaat cttgagggtt aggttgcggc aatgcctaaa tatgcgatat ttctaaagcc
    18661 tcagggaaag gaattaaaga cttcagacga cctgcaaagg gtcgtctgaa accacgattt
    18721 ttgcatttac gcattccggc acatcatcca accgtttcgg cacattcctg ccgccgttga
    18781 cagcctataa tgaatccact tattcatcaa gcaaaggaat catctatgca aacccgcatc
    18841 ctctccgccg tactgctggc tttttcaacc gctgcctttg ccgggggcgc attcacgctg
    18901 caattcgaca acccgtccga agacggcggc ttcacgcaaa accagctttt gagcgcgcct
    18961 tacggatttg gctgttcggg cggcaatgct tcgcccgcgc tgtcgtggaa aaatccgccc
    19021 gccgggacaa aaagtttcgt cctgaccgtt tacgataaag acgcgccgac cggactgggc
    19081 tggatgcacc gggtggtcgc cgacattccc gccgatgtcc accgccgcaa cgcgacctcg
    19141 ctgcaattaa gccgctgcgc caacatcgcc gacgaccagt ccgcagccat atcggcggta
    19201 atcagtttgc ggatttgccg catcaggttg ccgcctccgt acacggcaaa accgatgccg
    19261 tcatgctgca accacgccaa cacgccgcaa agtgcggcct ccgccacatt gtgcggcact
    19321 ttttcatccg ccagcaccgc agccgcataa tcaaacgccg cgttcatccg cccgggatgc
    19381 ggcagcttca ccgcatcgca cactgcctgt accgtcccgt attgtgcggc aaacctttct
    19441 acggtttcct gttcaaaagc aatccattgc gcctgataga ggccgtctga atcgggaata
    19501 ttaataacgt caaacgtctg tccgcctgcc aacgcaaccg cctcacccgc cgcaatcctc
    19561 aattcgcgcc tcgcccaccg cagcttcttg cttccgcgcc gcacgataag cacagccgat
    19621 tcatataccg ccacgctgcg gtacaaggcg gtatgatgtt gcacgatgcc gcctaaagca
    19681 cccaatcgtt cgcgtgtatg aaagtaattt gacggcacta ttatctccaa cacgcagggc
    19741 cgcacataag gcgcagccct gtttaccctg atttggaagg ggttacttcc caaaaataaa
    19801 ccaaaatccc actgccccga atggccgggt ttcaaccata ttccgtcggt gtgcctgcaa
    19861 cttgactccc tcccctgtgg gggagggttg gggagagggt aaaacggggc agatacagac
    19921 aatatttccg ttaccgcccc gatgccctct ccctaaccct ctcccacggg agagggaatg
    19981 gattgccgtt gaaataaatc atcctacata aaaatcaatg tgttatctca aacccacgtt
    20041 aggtctaata aaatggtcgg acatccttat ccgacagccc atcttctttt cagacggcat
    20101 tgcaaattta agtttgacgt gcgttcaaaa taaggcagtt aatgcgaagc gaaattccgt
    20161 cggcgtacct gcaacttggc ccctccccta tgggggaggg tcggggagag ggtaaaacgg
    20221 cagatacaga caatatttcc gttgccgccc cgatgccctc tccctagccc tctcccacga
    20281 aagagggaat ggattgccgt ttaaataaaa aaatctacat aaaaatcaat gtgttatagt
    20341 ggattaaatt taaatcagga caaggcgacg aagccgcaga cagtacaaat agtacggcaa
    20401 ggcgaggcaa cgccgtactg gtttaaattt aatccactat atctcaaacc cacgttaggt
    20461 ctaagcaaat ggtcggacat ccgtatccgg caagcaagct gctttcagac ggcatttcca
    20521 gccaacaacc gcgccaatat cccctcatac accgcagaca gcttcggaat gtcgtttagc
    20581 cgcacgtttt cgttgatttg gtggatggtc gcattggacg ggcctaattc gatgagttct
    20641 tttgcaatgg ctttgatgaa gcgtccgtcc gaagtgccgc cggtggtgga caattcggcc
    20701 tcaatgccgc agatttcggc aatggcggcg cgtgccacgt cggtcagttt gcccgcttgg
    20761 gtcagaaagg gctgccccga acacgaccac tgcaaatcgt attgcacacc gtgtttgtcc
    20821 aaaatggcgt gaacacgttg tttcagccct gcttcggtgg actcggtgga gaaacggaaa
    20881 ttgaatttga cattcagctc gcccggaatg acgttggtcg cgcctgtgcc gccgttgata
    20941 ttggaaatct ggaaactggt cggcgggaag tattcgttgc cttcgtccca gacttcctgc
    21001 gtcagctcta acaaggccgg ggcaaaagta tggacgggat tgattgccaa atgcggatag
    21061 gcaatatgac cttgcttgcc ttttacggtc aggttgcccg acagcgagcc gcgccggccg
    21121 tttttaatca tatcgcccaa tttgtccacg gctgtcggtt cgccgacgat gcagtagtcg
    21181 ataagctcgt cgcgcgcttt caatacatcg acgactttgg tcgtgccgtc caacgcgtcg
    21241 ccctcttcgt cggaagtaat cagaagcgca atgctgcctt ggtggtcggg atgttcggca
    21301 acgaagcgtt cacaggcggt aacgaagcag gcgatgctgg ttttcatgtc tgccgcgccg
    21361 cgcccgtata atcttccgtc gcgctcggcc ggttcgaacg ggggcgaatc ccatttttca
    21421 acagggccgg tcggtacaac gtcggtatgc cctgcaaaac agacgacggg agctttcgtg
    21481 ccgcgtcgca accagatgtt tttggtgtcg ccgaaatgga gttcttcagc cgcaaaaccg
    21541 attttgtgca ggcgttcggc aagcagtttt tggcaatttt ggtcatccgg cgtaacggac
    21601 ggtcgggcaa tcaattcttt ggcaagctct agggatcggg tttcggtcat ttttcggctt
    21661 ttctgtttga gatgccgtct gaacggcgtt tcagacggca tttgatgttg aatggaacgg
    21721 gctaacgata gcgaagcggg ttcttcaggt caagcaccat gacacgcttc gtaaagcggc
    21781 agcaaatctt ccaccgtttc cgctatccat ttcgcgacat cctgcctgcc caaatcgtcg
    21841 cgttcgatgt gtttgccgat gcagaaaaag tcttcgtcgt tttgcaactt tcggtcggac
    21901 tcgttttgtt gcgcgacggt gcggtagtcg tcgtattcgc tttccgcacc gtgccacata
    21961 tcgaaagaag cgtatttttc ggtatcaaaa ttatccaacc agcggttgta atcaggcagc
    22021 gcgatggggg aaatatcggc tttatagcag tgccaatcca agctgacgct cagacggcgg
    22081 cggttgagca gtatcgacaa aatcgctgcg gaatttttat attgttcgta tttgaagtag
    22141 gcaaagaaat gggcgcgaac ctgccagccg ttacaccagc gttcgatgtg cggcggcgca
    22201 aacggcgcac ccaattcggc ggcaacctgc tgaatcagct gctgccatat ctgccagttt
    22261 tccttgtagt cggctttgat ttgcggaatg ctttcaggcc ggtatttttt aagctgggca
    22321 aactggaaaa acgggatatt gaacaaatcg caacttttcg gggtcagcat aatatatcct
    22381 tgagacgatt gtttcagacg gcattatttg cgccgacgcg ccgccatgat ttcaccgatt
    22441 tcggtcagtt tttcttttgg gataaaggtg ttgcccatat caaacagcgg ctcttcaatc
    22501 gccagatgca catcgtaacc tgccacaaag cgtttgaatg cttcggcatc ggggatatag
    22561 gcattgtctg cttcgagctt ggcaaattcg gcggaaacag ccgcccagtt gtcgtgcagc
    22621 ccgatatgtt ggcgtaaaag ctcgtccaca ccttcacgtg cttgcggcgc gtattgcagc
    22681 agcagcggga aaaagttttc ttcttcgtct tcatggtgca gcggcgcggc aacgttgaaa
    22741 taccgggaga tttggcggat ggtttgcaaa acaatctgat tgcagccgtt ttcggcgatg
    22801 tagtccggca gcatggcgac ttggccgcaa aaacggcgta ctttgccgtg gcaggcatac
    22861 agcatttcaa tcggttcggc aaaggtaacg ctttgggttt caaacggatt catgttttcg
    22921 ttctcaacgg tgcacttttc aagcagtcat tttataataa aacagcctgc acaaagcagg
    22981 ctgttcgtct tttgaaactt taagcggatt aatcgaccaa agtcactttg ccgttcatca
    23041 aagcaccgtg acccgggaag gtgcaggcaa atttgtagtc gccgtcagcc aatttggcag
    23101 gatctagagt cagggaagac tcttcgccgc cgccgatcag tttggtgtgg gcaacaacgc
    23161 gcgcatcgtc aggtttgaca tagtcggtat cggcagcacc tacgccgtct ttaaatacgc
    23221 cgtccatgtc ttcagctttg gcaatcacaa ggttgtgacc catgctggct ttgggttgcg
    23281 taccggtatg tttcagagtg atggtaaact ctttacatgc tttgctgact tggatgtctt
    23341 tggtgttgaa ctgcatattg tcgttggatt cgacagttgc cgcacagttg ccgacagcag
    23401 gggcttcggc agcatctgca ggagcagctt cggcggcagg tgcttcggaa gcgggtgctt
    23461 cagcagcagg agttgcctcg gcagcaggcg cggcaggttc ttgagagcag gcagccaaac
    23521 cgataacggc agcagaaatc agagccagat acgctttcat aacaaatctc caatcgataa
    23581 aataatattc ggttttacag aaatcaaagt gcaaccgcca ttaacaaaac cttgaaaaag
    23641 attccgccgc gttgcacaaa cagatgtttc ggggcggcat tttgctacaa atttcatttg
    23701 aaatcaaagc ctgtctgcaa gtttacaatc gtttacttta aaaggggcaa ttttaccccg
    23761 aacctatttc tttagtatta gacccattat cctttacttc ttaatattaa cggatgttta
    23821 cacaaattcc cgtatacatt ttatgcgcca tgccttctaa ccaagtttgc caatgcctcc
    23881 gccaattcgg gatgccgttt ttccaacttt gccgccgccg aaccgaaact ctccagcgca
    23941 gccttactca aatgcagggt attggttttc ggcggttttt ccggtttcgg gaccagcctg
    24001 accgaaacag agcgtatcga agcgtcaagc cctgccagct gcggcaatac cgacggtgca
    24061 atcattttca agcgcgatgc cgccatattg tttgccgcca aaagaatcag cctgccgtct
    24121 tcgatacatg ccgtctgaaa atgcgggtgc aggttggcag gcagcagttt tttcacggcg
    24181 gcatccaacc gccgccactg tcccgcctgt ttcaaaagtc cggaaagcag cgcgtcccgc
    24241 ctgcccaact gttccaaatt cataaaacat acacccaaaa agattgaaat accgcaaacg
    24301 cgcctttatt tcagacggca ttagcacttt gcacaaacgc ttgtgttaaa atcgcgtttt
    24361 cgcccactat tatatcaggc gcaggaatta ttcatgctga caaacattgc caagaaaatc
    24421 ttcggcagcc gcaacgaccg cttgctgaaa caataccgta aatccgttgc tagaatcaac
    24481 gcgctcgaag aacagatgca agccctaagc gatgctgatc tgcaagccaa aactgccgaa
    24541 ttcaaacaac gcctcgccga cggtcagact ttggacggca ttttgcccga agccttcgcc
    24601 gtctgccgcg aagcgtcccg ccgcaccctc ggtatgcgcc acttcgacgt gcagcttatc
    24661 ggcggtatgg tgctgcacga cggcaaaatc gccgaaatgc gtaccggcga aggcaaaacc
    24721 ctggtcgcca ccctcgccgt ctatctcaac gcgctggccg gcaaaggcgt acacgtcgtt
    24781 accgtcaacg actacctcgc ctcgcgcgac gcaggcatta tggagccgct ctacaatttc
    24841 ctcggcctta ccgtgggcgt gattatttca gatatgcagc cgttcgaccg tcaaaacgcc
    24901 tatgccgccg atattaccta cggcaccaat aatgaattcg gcttcgacta cctgcgcgac
    24961 aatatggtta ccgaccaata cgacaaagtg cagcgcgaat tgaattttgc cgttgtcgat
    25021 gaagtggatt ccatcttgat tgacgaagcg cgcactccgc tgattatctc cggccaggca
    25081 gacgacaaca tccagttgta ccaaatcatg aacaccgtcc cgccccacct cgtccgccaa
    25141 gaaaccgaag aaggcgaagg cgactactgg gtagatgaaa aggcgcatca ggtcatcctg
    25201 agcgaagccg gccacgaaca cgccgagcaa atcctgaccc aaatgggatt gctggcagaa
    25261 aacgactccc tctattccgc cgccaatatc gccctgatgc accaccttat ggcggcattg
    25321 cgcgcgcata cgttattcca caaagaccaa cattacgtta tccaagacgg cgaaatcgtc
    25381 atcgttgacg aatttacagg ccgtctgatg tccggccgcc gctggtcgga gggtctgcat
    25441 caagccgtcg aagccaaaga aggcgtggaa atcaaacgcg aaaaccaaac ccttgcctcc
    25501 atcaccttcc aaaactattt ccgcctgtac accaagctct ccggcatgac cggtacggca
    25561 gataccgagg ctttcgagtt ccaaagcatc tacaacctcg aaaccgtcat catcccgacc
    25621 aaccgccccg tacagcgcaa agacttcaac gaccagattt tccgttccgc cgaagaaaaa
    25681 ttcgaagccg tcgtcaaaga catcgaagaa tgccacaaac gcgggcagcc cgtcctcgtc
    25741 ggtacgacca gcatcgaaaa ctccgaactg gtctcccacc ttctgcaaaa agccggactg
    25801 ccgcacaacg tcctcaacgc caaagaacac gaacgcgaag cgctgattgt cgcccaggca
    25861 ggcaaagtcg gcgcgattac cgttgccacc aatatggcgg gacgcggtac ggacatcgtt
    25921 ttaggcggca acctgaagca ccaaaccgat gccatccgtg ccgacgaagc cttgagcgac
    25981 gaagagaaac aggcacaaat ctccgcactc gaagacggct ggcaggcaga acacgacaaa
    26041 gtgatggaag caggcggttt gcacatcatc ggtacggaac gccacgaaag ccgccgtatc
    26101 gacaaccagt tgcgcggacg ctccggccgt cagggcgacc ccggatccag ccgtttctac
    26161 ctctcgtttg aagacccgct gctgcgccta ttcgcactcg accgcgccgc cgccatcctc
    26221 aaccgcctcg cccccgaacg cggcgttgcc atcgagcaca acctgctgac gcgccaaatc
    26281 gaaggggcgc aacgcaaagt cgaaggcaga aacttcgata tgcgcaaaca ggttttggaa
    26341 tacgacgacg ttgccaacga gcaacgtaaa gtcatttaca gccagcgcaa cgaaattctg
    26401 accagcaaag acatcagcga cctgatgaag gaaatccgtt ctgatgtcgt cagcgacctc
    26461 gtggatacct atatgccgcc cgacagcatg gaagaacaat gggacatccc gactttagag
    26521 aaccgtctgg ctgccgaatt cagactgcac gaagacattc aatcctggct gaaagcagac
    26581 aatacaattg acggtcaaga catcaaagag cgcctgatcg aacgcatcga aaacgaatat
    26641 gccgccaaaa ccgaactggt cggtaagcag gcaatggccg atttcgagcg caacgtgatg
    26701 ttgcaggtca tcgacaacca atggcgcgaa cacctcgccg ctatggacta cctgcgacaa
    26761 ggcatccacc ttcgcagcta tgcccaaaaa aatccgaagc aggaatataa acgtgaagcc
    26821 tttaccatgt tccaagacct gtggaacggc atcaaattcc atattgcctc cctgcttacc
    26881 tcggttcaaa tcgaacaaaa ccctgtcgcg gtggttgaag agcaacccat cggcaacatc
    26941 cagtccatcc attccgaatc gcccgatatg gaagaacttt tgggtcagtc gcaaaccgat
    27001 ttggttaccg aggcctttaa tcccgatgga acggatttca gccccgaagc cttggaagcg
    27061 cggggacaaa tcgtccaccg caacgacccc tgcccctgcg gcagcggttt gaaatacaaa
    27121 caatgccacg gcaaactggc ttgagcgttt gaacgcaaat gccgtctgaa catccggttt
    27181 ccggcttcag acggcatttt gcctgaaccg ccacatccga ccgccattcc gaaaaatccc
    27241 gatttcgtac cgtccaaact aaaaacaggc atccgccccg ccgaactatg attccatccg
    27301 acttcattga cgaacttctg gccaaaaccg atattgtcga catcatcgac gaacaggttc
    27361 cgctgaaaaa aggcggggcg aactatatgg cgtgttgccc gttccacaag gaaaaaacgc
    27421 cgtcgttttc ggtcagtccg accaagcagt tttaccactg tttcagttgc ggggcgcacg
    27481 gttcggcgat tggttttgtg atggaacatc aggggctgtc gtttccggag gcagtgcagt
    27541 tccttgccga ccgcgtgggt atgattgtgc ctaaagtgcg cgggcagaac gataatcccg
    27601 aagtccgtgc cgaacgtaag aaaaagcagc agacgctgga agaaacgaca gcggcggcgg
    27661 ctgattttta tgcgcaacag ttgaaattca atccgacggc gaaggcttat ttggacaagc
    27721 gcggcttgag cgcggaagtc atcgcgcatt atggtttggg ctacgcgccc gacggctggc
    27781 agcctttggc gcaagtattc caaccgtacc cgaataccgc gttggtggat acgggtatgg
    27841 tgattgacaa tgaggggcgg tattacgacc gattccgtca tcggattatg ttccccatcc
    27901 gcaatccgcg cgggcaggtc atcggtttcg gcggcagggt gctggacgac tccaaaccca
    27961 aatatctaaa ctctcccgat acgcctttgt ttgataaggg gaaaaacctt tacggattgt
    28021 atgaagggcg tgctgctgtc aaggaagtgg ggcggattct ggtggtcgaa ggctatatgg
    28081 acgtggtcgc gctggcacag ttcggcgtgg gctacggcgt ggcggccttg ggcacggcaa
    28141 caacagcgga acacgtcaaa atcctgatgc gtcaggcgga cagtatttat ttctgtttcg
    28201 acggcgacag cgcggggcgc aaagcggctt ggcgcgcgct ggaaaacgcg ttgccgcagt
    28261 tgaaagacga caaatcgctg cattttttgt tcctgcccga ggaacacgac cccgacagtt
    28321 acatccgtgc ctacggcaaa gcgcaatttg aagacgcgct tctgaatcaa agcaagcctt
    28381 tgtcggagta tttctgggaa cacctttcag acggcattca tctcaatacg caggaaggca
    28441 aggcggaatt ggtgaaaacc agttcgccgc ttttggcgca gattaccgcg ccggcattgg
    28501 cttatttgtt aaaacaacgg cttagcgaac tggtcggcat cgaccccgac aacctcgcgc
    28561 aactgctcgg acaggaagcg ccgaagcggc acgtcaaaca aaaaaactac aaactgcctc
    28621 cgatttccgt caaacagccc gtcatgctga cgctggtaca gcggcaaatc cgcagcctct
    28681 tgataaatcc ggattgggct gcatatatag acctgcccga ttatctggcg ttggacggtg
    28741 atttcgcctg ccttgccaat cttgccgaat cgattaaaaa ccatgccgcc gtacccgaaa
    28801 ccgctcaggt tttagagtat atgcgcggct cgccttacga agaaacgata acccgaatct
    28861 tccattcaac gcaccaatcg gaagaaatga acagcagcag tgaagaagat tgcgagaatt
    28921 tccaaatcgg catgaaaaaa ctgctcaatg agttaaaata cagccaaatc gaagcattga
    28981 aacaaaaaag cctgcaatcc ggcttaaatg aaagcgagaa aaaacttttg ctgtcgctgc
    29041 tgaccgcaaa acaaaattga ccggcggatt ccgccatccg taaaccgtta tgccgtctga
    29101 aaagcattca ccccggctgc aacaacgaca cctgcagaac acccatccca aaaagccttc
    29161 agacggcatc agagtaccct actctgccac gccttcaggt gcgtccaaac gcaaaccgtc
    29221 ggcatcttgc caacagaaag cagacaatgt ccaaaaacca aaatcacgaa gaataccaag
    29281 acgatgcccg ccccctgacg attgaggaac aacgcgcgcg cctgcgccaa ctcatcatta
    29341 tgggcaaaga acgcggctac atcacctact ccgaaatcaa cgacgcactg cccgacgata
    29401 tgtccgatgc cgatcaaatc gacaacatcg tcagtatgat ttccggtttg ggcatccaag
    29461 ttaccgaaca cgcccccgat gcggaagaca tattgttaag cgacaatgcc gccgttaccg
    29521 acgatgatgc cgtcgaagaa gccgaggccg ccctttccag cgcagattcc gagttcggca
    29581 ggacgaccga ccccgtccgt atgtatatgc gcgaaatggg gcaggtcgac ctgctgaccc
    29641 gcgaagacga aatcatcatc gccaaaaaaa tcgaaaacgc cctgaaaaat atggttcagg
    29701 ccatctccgc ctgcccgggt tccattgcgg aaatcttaga actcatcgaa aaaatccgca
    29761 aagacgaaat ccgcgtcgac gaagtcgtag aagccattat cgacccgaat gaagtattgc
    29821 tcaacgaatt gggcttgggg cacttggaaa ccacagcacc cgagaaacct tccaacgaca
    29881 attcggacga aaacgaagac gacgaagaat cggaagaaga tgcggatgaa atctcggcag
    29941 ccaatctcgc cgaattgaaa caaaaagtca tcggccactt tgcccaaatc gaaaaagact
    30001 acaaaaaaat gattggccgt ttggaaaaac accacagccg gcacaaagac tatctcgcct
    30061 accgcgacgc gattgccaac aaactgctgg aagtccgttt cgccacccgg caaatcgaca
    30121 gcctcagcag cagcctgcgc ggaaaagtag aaaacatccg caaactcgaa cgcgaaatcc
    30181 gcgacatctg cctcgaccgc gtccatatgg agcgcgacta cttcatccaa aacttcctgc
    30241 ccgaaatcac caatctggaa tggattgaag aagaaatcgc caaaggcagg gtttggagcg
    30301 acgcgctcga ccgcttccgc cacgccatcc tcgaaaaaca aaccgagttg gcggatatgg
    30361 aaaaagaaac ccgcatttcc atcgaagagt tgaaagaaat caacaaaaat atggtgtcga
    30421 gcgaaaaaga aaccgcagcc gccaaacagg aaatgattca ggcaaacttg cgcctcgtga
    30481 tttccatcgc caaaaaatac accaaccggg gcttacaatt ccttgatctg attcaggaag
    30541 gcaacatcgg tttgatgaaa gcggtcgata agttcgaata ccgcagaggc tataaattct
    30601 ccacctacgc aacctggtgg atccgccagg caattacacg ctcgattgcc gatcaggcgc
    30661 gtaccatccg cattccggta catatgattg aaaccatcaa caagatgaac cgcatctcgc
    30721 gccaacacct tcaagaaacc ggcgaagaac ccgattccgc caaacttgcc gaactgatgc
    30781 agatgcccga agacaaaatc cgcaaaatca tgaaaatcgc caaagagccg atttcgatgg
    30841 aaacccccat cggcgacgac gacgattcgc acttgggcga cttcatcgaa gatgccaaca
    30901 atgttgcgcc ggccgatgcg gcaatgtaca ccagcctgca cgaagtaacc aaagaaatcc
    30961 tcgaaagcct gaccccgcgt gaggcaaaag tcctgcgtat gcgtttcggc atcgatatga
    31021 acaccgacca cacgctggaa gaagtcggca gacagtttga cgtaacgcgc gaacgcatcc
    31081 gacaaatcga ggcaaaagca ctccgcaagc tgcggcatcc gacaagaagc gaccgtttga
    31141 gaagtttctt ggacagcgaa gacagcaagc tgtaaaccaa aaaaccgcag gtttcaaata
    31201 cctgcggttt tttcttacac aataaacaac gcttccacat atcccacact cctatcccga
    31261 gacctttgca aaattcccca aaatccccta aattcccacc aagacattta ggggattttc
    31321 catgagcacc ttcttccaac aaaccgccca agccatgatt gccaaacaca tcgaccgttt
    31381 cccgctattg aagttggacc aagtgattga ttggcagccg atcgaacaat acctgaaccg
    31441 tcaaagaatc cgttacgtcc gagaccaccg cggtcgtccc gcctatcccc tgttgtccat
    31501 gttcaaagcc gtcctgctcg gacaatggca cagtctctcc gatcccgaac tcgaacacag
    31561 cctcatcacc cgcatcgatt tcaacctgtt ttgccgtttt gacgaactga gcatccctga
    31621 ttacagcacc ttatgccgct accgcaattg gctggcgcaa gacgacaccc tgtccgaatt
    31681 gctcaaactg atcaaccgac aactgaccga aaaaggccta aaagtagaga aagcatccgc
    31741 cgccgtcgtt gacgccacca ttattcagac cgccggcagc aaacagcgtc aggccataga
    31801 agtcgatgaa gaaggacaaa tcagcggcca aaccacaccg agtaaggaca aagatgcccg
    31861 ttggatctct acaaactcgg ttacaaacaa catacccgta ccgatgcgga aggctatatc
    31921 gagaaactgc acattacccc cgccaatgcc aatgagtgca aacacctgtc gccgttgttg
    31981 gaaggactgc ccgaaggtac gaccgtctat gccgataaag gctacgacgg tgcggaaaac
    32041 cggcaacatc tggaagaaca tcagttgtta gacggcatta tgcgcaaagc ctgccgcaac
    32101 cgtccgctgt cggaaacgca aaccaaacgc aaccggtatt tgtcgaagac ccgttatgtg
    32161 gtcgaacaaa gcttcggtac gctgcaccgt aaattccgct atgcgcgggc agcctatttc
    32221 gggctgctca aagtgagtgc gcaaagccac ctgaaagcga tgtgtttgaa cctgttgaaa
    32281 gcggccaaca ggctaagtgc gcccgctgcc gcctaaaagg cagcccggat gcctgattat
    32341 cgggtatccg gggaggatta agggggcgtt tgggtagaat taggaggtat ctggggcgaa
    32401 aacagccgaa aacctgtgtt tggatttcgg ctgtcgggag ggaaaggaat tttgcaaagg
    32461 tctcagagtg agtttatttt ggggcggcgg caggtcgggg gcaagcggcg tggggcttgg
    32521 ttgtggtttt taggtttggg ggtgggtaaa aaatgccgta cccggcgggc aggcttcgca
    32581 ctctgaaggt ttcagacggc gtttgttttt tctgtccaat cgaggaactg ccgccatttt
    32641 tccagcggca tatcggcccg ccgcgtttcg gtatcgggtt cggcttccca gcggcctacc
    32701 tgtatgtagt gcttcacccc gacgatttgc gccaactcgg cctgtatcag tttgcagcgg
    32761 ttgcgcaggt tgtaaggtgt gtagccgagt tccatgtcgt tgcggtttat tttgtttcgc
    32821 atattttttt gactgcccgg cggcaggttt cggtaaggac ggcggcaaat cgggcttcgt
    32881 ctgccggttt tccgtcgaaa aaaataaaat cgtaaagggt gatgccgttt tcggaatggc
    32941 ggtaatgccc gcccgggcat tcgccgtctg tctgcctgtg gattttggca atcaggcggg
    33001 gatagcggca atgggtaatg aaacggcttg cgccgtcttg cccgataatc cattcggggt
    33061 agcggttgaa tagtgcggct ctgttcattt tgttcggggg ataaagcctc tcgcggggct
    33121 tgtggtcagg caaatttgaa tatcagtgcc aacacggcgg cgatggcggt aaccagcccg
    33181 gtggccgcca tcatgggata ccatcgggat tcttgggcta tttttacgga ttcggcgttg
    33241 attttgtgcg cgtctgcaat gattttggcg atttcgacat ctacttttct cagactggag
    33301 tgtttcattt cttgttcgat tatgttcatc gtacttcctt tcgtttttgg cggttgccgc
    33361 cgcttgtcgg atggtaggat gtctgccatg tgtatatatt gatacctttt aggttttatt
    33421 gcaagtgttt tgggcggcgg cttcgtatgc ttggcggtgg cggcggctgt accattcggc
    33481 gagttcgcgc cgctcttgga ggcggtagct gtcggcggtc aggtagtggt gcaggcttga
    33541 gaagccggcg cgttggaggg cggcgcggct gatgtggccg agggcgaggt ctgttgtgag
    33601 ggtgtctttt ccggcggtca ggctgatgtt ggtgaagagg tggcggaatc cgtgcattgt
    33661 gtgtttggat ttgccggggg tgctgccgtc atagcccagt cgtcggatgg cgttgtgggc
    33721 gaatttgatg ctgatgtggt cgggatgggg ggcgggtttg cgccgtgggc ggatgcctgg
    33781 gaaaaggtgg atgttgtcgc cggtctgtgt gtgcagctct cggagtattt ctaccgccca
    33841 gtccgacagt gggacggtaa aggggtgttt ggtcttcatg tcggcggcgg ggatgtgcca
    33901 taaccgggcg gtgaggtcga tgtcttgcca gcgggcggaa agcagtgcgg acggacggga
    33961 tgacttcccc ggtttcccca tcaatccgcg ccgcctcgat ttttccgttg gcgcgcagca
    34021 tttccaccgc ctgccgcacc gacatcaacc gcttgccgtt gcgcctcatc gcctccacca
    34081 aaaccgccga aatcaatttg gcttcttccg gtttaagctc cgtcttgccc gcatcgctgc
    34141 gccgtttgcg cgtcggcttg atgctgaccg cctccagctt gcggtatagc gtggcaaggc
    34201 tgatgcccaa ttcctgcgcc tgctgcttaa gatatgcaga gcgtgcgccg cgtcccattg
    34261 cttccgcctg attctcgact gccttaagac gctcaatcat tgccggattc atcgccttct
    34321 cccgtttcac cgcccaacca ttccggcaca ttgtctgtcg gtgcttcagt cggcagggca
    34381 tagctttcgc gcagttgctc gcagtccaaa ataatttgat tgagcgtgcc gaccatcttt
    34441 gcctgatggc tgatctcgtg tgcctcactg tgcgcattaa gttggtcgaa caaatctttc
    34501 aaacggctca cttgactgcg gataccgacc tcaaggcttg ttaactgcat cgccaactca
    34561 ctgcccacgt cttccgcctt cggctctctg acaacggttt gcttcttagc cagcttctcg
    34621 gtcagctcat caattttggc tgttttggtt ttcatcactt cgtctttggc ggcgaggttt
    34681 tcacggcttt cgcgcagggc gacgcgcagc tcgcgcaccg tcattcggtc cacatcgtca
    34741 aaggtcatgc cgttgacttc ttctccttcg gcaagtccca ccaacgtaac gtcttcttcg
    34801 accaagagtt ccagcagctt tgacttgccc aaatccatca gcttcggcgc ggctttctgc
    34861 atttgcgggg tcgcaaagcg gcgagtggct gacatcagac gtgatgtttc tgcgataccc
    34921 agcccaaatt ggcttttcac aatttccata aaccgtccgt gttccgtatg ctcttttaaa
    34981 ataatcagcg cacgtcccag ttcgaacatg ccttccatcg tctgccgtac tgcttgacga
    35041 ccgcgttcaa cccaacgctc ttcgctataa gtctcgccgt taccccactg ctccatcacc
    35101 agcacactgc gcatcgctgc ctgattgctt actttgtcgg ttgcgataat ttcaatttct
    35161 gtattcattt tttatctcca aagtttccga cgtcggaaac tttcaaaatc cgttaatcca
    35221 catcgacccg cttgccgatt tcggcaatct tgctttgcag ccgttcatgc tgctgcctga
    35281 accgctctgc gatttgcagg gttttgatgc cgtaggcgta gttgccgttt tcaagtttga
    35341 tgaccaatcc cgaggcaacc aaatcatcaa tatccctgct gacttgcgat ggcgtcagcc
    35401 ccagtccgac cgataaatcc ttattgctca gaccgataat cggatgctcg tcaagcgcga
    35461 taaagaccct caatagccgt tgtacccttt tactttctgc catccgcatc ctccttattt
    35521 cagtcccagc ttcttggcaa tttcatgccc cttgccgtaa ttgcctttgc gctgtccgcc
    35581 gaccaccaga tacacatcgc gcggcttaaa gccgttctct cttgcccact gcgccagcgt
    35641 ttggccgttt tgggcaaaat tttcttttag tttttcaaca gtatagccat agatagccct
    35701 ttcccattgt gataaaatag tgaactattt atgcactaga aattaattag tgcattacca
    35761 atgcgtgaat tatgagattt taaaatctct tttacaagga ttattttgtg atttctaaat
    35821 ctctttttgg taatcgtttg aaagaaaaaa gaaagttttt agggcttacc caagcacaag
    35881 ctgctgaaaa agcaggtatt gagcgcgaaa catggggcaa atacgaacgt ggcgtattta
    35941 tgcctagtgg agatgtattg ctctcttttt tgaatatggg aatagatgtg agcagcttat
    36001 ttgctgctga acagggaatt aggccgtctg aaaattctga aattagtaag gaagagtcag
    36061 agttattagg gtattaccgc caagccagcg ataacggcaa atttgtcatt ctcagtgtgg
    36121 caaaaggcgc agagaaaaaa gcagccgaaa atactcaagt ttcagccacg cataaaacag
    36181 cgtgattttt aaatccaaaa tggagacagt cttatgtttg actttgaaca acaaatcaaa
    36241 tggggtgagc gagcggaaga aatcgttaaa gaggcagcca cccagaacaa tatcgaaatt
    36301 cccgaacctt tggcttcggc attagctaag gcggtaaaag tgcattatct aagccaagct
    36361 ggtgtgtttt ctttggtaga agcctatgca gacacggtaa atccaactga aaaagaagta
    36421 gattaccaag caatcggcaa agagctattt gaaaaataaa aaatgccgtc tgaaattttc
    36481 agacggcatt taggacactc cgacaggcgg gctgctccgg tgttctgaaa cggatattaa
    36541 gcaattgaag cggccgtgca ttttaaaacg cattaaaaac taccccgaca tacaggtcat
    36601 cgggcaggtg gttcaggtgt tgaaagattt gaattgatgc cgtctgaatt tattttttcg
    36661 acacagatat taaaagagaa aggactgtac aaatggcaaa aaaactacta aaactattca
    36721 ttatttacgt gcgcagcaaa accatagctt gttcgattta gaggtagcat taagaagggt
    36781 tttgtcagct gctcctactg ttcaagatac acaaattgaa caatacaatc agcttaccca
    36841 aatcatgcat agaactttgg atcctaaaga tgtaaaaggc aattcaattg gtggattact
    36901 gatacacatt ggcagcggta caaaagatga acatatccgc actatgagca atagacctgt
    36961 acagcaagat gatcatggtg gaacacaagc tcctccaagt ggatattcct ttttgcgtaa
    37021 agaggctttt ttgtatattg ttggacacca tgtaattttt tgcggacatg gttttttgtc
    37081 agcatcaacg gttgcttctt atttaagttt acttagcaat aaattgagag agaacaatcc
    37141 ggatattgtt caatttaata ttgaattcaa agcagtaggt aattgcgaca agttgtcatt
    37201 gatacaacaa catggggtaa aaagtattct tttagatgtt tcggcatatc aattatccag
    37261 aaacagactg tatcaagata gccgttcaac cattgctaaa gcattaggga aagttggtag
    37321 tgtatttaca agcgaattga gtgatgagga attggaagca caatccgaaa ttcatatcaa
    37381 tttagaagtc ttgctaaacg gtaattcgag agccagtatt gaagcgcaat cgctgatgca
    37441 ggagcaggca gaggaaatta ttgatgatga aacggttaat caaggatttt ccattaccac
    37501 gcaacaaggt gaagtgattc agccctctga tgtaaaactg tctaaatctg ttagaatagg
    37561 acgatacgat gaagccaact cgttactccc tgactcggca tttacagcaa tcagtgaata
    37621 tttcctagaa ctccaaagca gaaacttaac tgaacaatga aaaaatatct gcccttacta
    37681 cgttttttgt tgattgcttg tatatcagca tttttagcat ggaaagggca accttttgtt
    37741 catgggaacg agaaggccgt tgatttaatt attaacgtat ttgcaatctt agccggtttt
    37801 ttaattgcca ttatgacatt gtttagtgat atgaggtttg atgaagatgc aaattggcga
    37861 caaattcaaa ttcgtgaagg cgtacaagaa caacggtata taaaacattc cttacttttt
    37921 tacacatatc ttgccgtatt agtttgtgtt tttattgtca tcttgttggc tcataaagaa
    37981 gaatacaaaa atggaccagc cattttttgg cttgaacgga gctatttatt tttagcctgt
    38041 atttccattt tttattctgt attcttacct gggaatttaa ttaaaagccg aaaagaagag
    38101 tttaaaaaat taatggaaaa gaagaaaccc aaaatctaga acgagtttta acccccttta
    38161 aaatcccatt cagacgacct tttctaaaat ccctgtattg atttttaatt caatacaggg
    38221 atttttccat gtcagacgaa ttcaaccaat tcatcaaccg cgtcctctcc cacgagggcg
    38281 gttacgtcaa ccaccctcaa gaccccggcg gcgaaaccaa ttggggcatc actaagcgta
    38341 ccgcaatggc aaacggcttt aacggttcca tgcgtgccat gacccgcgaa caggctatcg
    38401 gcatttaccg aaaggcgttt tgggagcgtt accgcgccga ccaaatgccg gaagcggtcg
    38461 cgttccaatt ttttgatgcc tgcgtcaacc acggttacgg caatgccgcc cgtatgctgc
    38521 aacgcgccgc aggcgtggcg gacgatggaa tcatcggcga aatcagcctc aaagccatca
    38581 attccctccc cgaaaacgac cttttattgc ggctcaacgc cgagcgtctg gtcttttata
    38641 ccaagctcgg tacattcacc tctttcggca agggctgggt acgccgtgtg gcgcaaaacc
    38701 tgattcacgc gtctgcagat aacactgatt aaagggagac aaaccatgtc aaaaaagtca
    38761 ctcatcgccc taatgaccgc agccatgcag cccgatttca gccacagcga cctaggcatt
    38821 cgctacgcca tgccgactca gggatgttgg acgcaagccc accgcaagag cggggtagcc
    38881 gccgcgaaac gcgcagccaa aaaaaagcgt cacaaataac cgcctttttc cgatggctgg
    38941 gcggcttggt ctctaatccg gccacaggga aaatcagcca taccaaactt tgggcaaacg
    39001 tcgccgcagc cgccatgact tggaagttcg tgcaggcggc ggacgcgccc gaatggcttt
    39061 ggtgggcata cggcgcgatg gtcggcgggt atgcattaat caaacgcggc atcgccgtcg
    39121 taccgcagtt ggcggaagtc aaaaaatccg caaatcagga aggggggcgg caatgattga
    39181 atttgtccga gccaaaaaac ggctgctttg ggcatttgtg cttttgcttg tgtggacgtg
    39241 cggttaccga tacgccgccg acaaggccga agcggaacaa accgccctga ttgccaccta
    39301 tcggcattct tctatggttg cggcggaaca atatgccttg cagcttaaaa aaacgcagga
    39361 cgaaaggcag cggtggtacg acttttccca aaaacaagga agaaagcccg tgaaaaaaca
    39421 gtatccgccg caaacgaaaa aagctggcta tctgaaaacc aaggaagaac tgcttgcgga
    39481 attggcttgc cttaaagcgg aaatggttgc cctaaaaaag cccgatgcct taatccatgg
    39541 gaaagaaatg cggcagaaag aacgcaactc gtcgcagggt taaggcaatg ccatccgttg
    39601 aactgctgtt ggagattgtc ggactgccac gcggcacctt ttattaccaa ttggccgtcc
    39661 aatcggcaga agacaaatat gccgatttga aacagcatat ccatgatatt tatcgacgac
    39721 ataagggaag atacggctac cggaggattg cagcagccat ccttcatgca ggaacaccgg
    39781 tcaatcacaa gaaagtcagc cgtctgatgg cgaagacggg gctgaaggca gtgatacggc
    39841 ggcgcaaata ccgctcgttc aaaggagaag tcggcaaaat tgcgccgaat atcctgcgac
    39901 gctgtttcca tgcagaaaag ccgaatgaga aatgggtaac ggacgttacc gagttcaatg
    39961 taggcggaga aaagatatac ctttctccga ttatggattt gtttaacggg gaaatcgtca
    40021 gttaccgtat tcaaacccgc ccgactttcg atttggccga cgagatactg aaaggtgcgc
    40081 cggagaaacc gggatcgtct gaaaagccga tactgcattc ggatcaaggt tggcaatatc
    40141 agatgttttt ttatagtgga ttaaatttaa atcaggacaa ggcgatgaag ccgcagacag
    40201 tacaaatagt acggcaaggc gaggcaacgc cgtactggtt taaatttaat ccactataca
    40261 acaacgatag aatcaagttg aaattaaaag ggctgagcct tgttcagtac agaattcagt
    40321 ccctgaaagc cgcttgatta aactgtccga ctttttgggg tcagttcggc ttcggcattt
    40381 ttttatccgt ttgggggtaa cttgtttgga aagctgcaag cttataaata aaggattaca
    40441 tttaagtttt gggtaacctt tttaaaaaaa tgcgtgatga cttttgcatt tttaaggcgt
    40501 tttttggggt aattcgtgaa aagttacccc aaaagttacc ccataaatgg cgaaaactca
    40561 agcatacgcc agcatattca aagcaacaaa aaagccttga aactgttgaa gttcaaggct
    40621 tttttgtgtt gcaggatact gctggaaata gggtatggtg gaggcggggg gaatcgaacc
    40681 cccgtccgaa agtcctctac aaagcgttct acatacttag ttgtgtctat ttgaaaatct
    40741 tatttccatc atgccgacca acaggccttt tggaaaccag ttaccttaaa tctcatttcc
    40801 tgccaagtaa cccggtagga aaccagtcaa tgtaagatga cgttgcggtg gctttcgcca
    40861 cacagcccat tgaccgactg ctgcaacggc tagccttaag cggctaaagc gtaagtttcg
    40921 tcgtttgcga ctatttgaat tcagtgtttt acgggaatct gagaccccgg tatgcccgca
    40981 tctgcttcgc aacccccgtc gaaaccaagg tcgcccccag aaatggtttg caaattatac
    41041 ggatattgtg cggtgctgcc aagtctgtcg gagaaatttg tcagtcttgc tgccttaatt
    41101 tgcgtttgag caggatgcgg acgcagccgt cgttgccttc ccggggttcg gcgtaggcga
    41161 gtacgtcggg gtgttgcatt agccagtttc gggtcatatt tttcagaacg ggtttgtagc
    41221 ctttggaacc taatccgctg ccgtggatga tttcgccgca tacgccgcgt ttttgggtga
    41281 atgcgatgaa ttcgttgagg actttttggg cttcttcctg tgtgtagccg tgcaggtcga
    41341 catcggtaac gacgggatag tatccgtttt tcaggcgttg gatgtcgttt tttccctgcc
    41401 cgtttttgct gaagctggcg ggcgggtcgt tgtatgtgct gccgatgtag aagtagtttt
    41461 cttcgtcggc gcggttgtct ttgggacgga ctttgatggg ggttttgtcg ggcggcgcat
    41521 aatattgctg ccggtttttt aatggggaga cttgtccgac tgcttgtgaa aaatcgaaat
    41581 cctgttcttg tttttgcttg tttttttcgg ctgcctgttt ttctgccgct tctttttggg
    41641 cttgtttgcc cagttgtttg aggatgtttt ggaagtcggt attcatattt tttcctgtta
    41701 tttgtccgat ggctgtttcg ggcggggttt taatttgccg gaatgtttgc caatcggggg
    41761 aggatgattt tgttgcctgc gtatgttttt tgaaagtgtg attgtatatc aaaaagaaat
    41821 gcggcaaccg tcggcagtgt tgattgccgg aaatgcggac cggtcgaacc gatatgcccg
    41881 aacgcctgat aaagttttaa aaacctgcct tgcgaagcag gctgacgtgt tttgccaatc
    41941 ttgaattgcc ggaaacgcga aacacggaaa tctgatg
//
LOCUS       DAWFJR010000007        40797 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWFJR010000007 DAWFJR010000000
VERSION     DAWFJR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.007"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            39..590
                     /locus_tag="WGR04_000312"
     CDS             39..590
                     /locus_tag="WGR04_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793947.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKAGGAVKAGG"
     gene            complement(856..1405)
                     /locus_tag="WGR04_000313"
                     /pseudo
     CDS             complement(856..1405)
                     /locus_tag="WGR04_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1807..2526)
                     /locus_tag="WGR04_000314"
     CDS             complement(1807..2526)
                     /locus_tag="WGR04_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793948.1"
                     /translation="MLAIYSINILIYNEKMFFRVTLYFVKNFVSFSKTRKSNEMMQKH
                     KCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSISYINTERNQSEFANPIYVQ
                     TSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQAEMKETQGEFVPIIAAAAF
                     GGALGAWGYHGANLYNHGKLGTAQGAATAAGIGAATGVAATGLAAAAGGGLAGNLAWR
                     PGIHALGFGANAANNAVNSRK"
     gene            complement(2807..3649)
                     /locus_tag="WGR04_000315"
     CDS             complement(2807..3649)
                     /locus_tag="WGR04_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0793949.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPILVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLAGGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETRVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3715..3855
                     /locus_tag="WGR04_000316"
     CDS             3715..3855
                     /locus_tag="WGR04_000316"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793950.1"
                     /translation="MPSEKYGIRAVKLCGGKCAIIRAVLPLLRSCSANIRAAWTYNRI
                     PA"
     gene            3884..4804
                     /gene="pyrB"
                     /locus_tag="WGR04_000317"
     CDS             3884..4804
                     /gene="pyrB"
                     /locus_tag="WGR04_000317"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ0793951.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLAHAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4814..5272
                     /locus_tag="WGR04_000318"
     CDS             4814..5272
                     /locus_tag="WGR04_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0793952.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDHFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            5337..6002
                     /locus_tag="WGR04_000319"
     CDS             5337..6002
                     /locus_tag="WGR04_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793953.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVRNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACSSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(6065..7282)
                     /locus_tag="WGR04_000320"
     CDS             complement(6065..7282)
                     /locus_tag="WGR04_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0793954.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            7424..7969
                     /gene="def"
                     /locus_tag="WGR04_000321"
     CDS             7424..7969
                     /gene="def"
                     /locus_tag="WGR04_000321"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ0793955.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            8056..8982
                     /locus_tag="WGR04_000322"
     CDS             8056..8982
                     /locus_tag="WGR04_000322"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ0793956.1"
                     /translation="MKVIFAGTPDFAAAALRAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVEQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQDVLDVPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDAGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLRKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            9061..10320
                     /gene="rsmB"
                     /locus_tag="WGR04_000323"
     CDS             9061..10320
                     /gene="rsmB"
                     /locus_tag="WGR04_000323"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ0793957.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAILRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNITAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIPARALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            10286..10885
                     /locus_tag="WGR04_000324"
     CDS             10286..10885
                     /locus_tag="WGR04_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ0793958.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10885..13005
                     /locus_tag="WGR04_000325"
     CDS             10885..13005
                     /locus_tag="WGR04_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ0793959.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAVPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHKLDQPFPGKARWEKIQRAGSVRDLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATL
                     LIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAE
                     QILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALI
                     GDVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVK
                     SEVGRDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRI
                     SLSNQDAGGACVRIILPKTVKTYA"
     gene            12998..14275
                     /locus_tag="WGR04_000326"
     CDS             12998..14275
                     /locus_tag="WGR04_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ0793960.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSAVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVEAAAKRTSPVF
                     LTGEVGSPFETVARYFHKNGTPWVSPARVEYLIDMPTELLQKAEGGVLYVGDIAQYSR
                     NIQTGIAFIIGKAERCRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            14366..15559
                     /gene="dprA"
                     /locus_tag="WGR04_000327"
     CDS             14366..15559
                     /gene="dprA"
                     /locus_tag="WGR04_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ0793961.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRRFGSAQNALSAPAEQV
                     AALIRHKQALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGITA
                     PPVLFLRGNVQLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGQNIPVVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKGIPEKRITAVQTASDQLSLPEGKMPS
                     EKTENRPVGGSILDRMGFDPVHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRY
                     QRIRT"
     gene            15585..16046
                     /locus_tag="WGR04_000328"
     CDS             15585..16046
                     /locus_tag="WGR04_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ0793962.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            16118..18424
                     /gene="topA"
                     /locus_tag="WGR04_000329"
     CDS             16118..18424
                     /gene="topA"
                     /locus_tag="WGR04_000329"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ0793963.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTSQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMMP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIAGGKRQWVPVMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAESCPKCAWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(18497..19192)
                     /locus_tag="WGR04_000330"
     CDS             complement(18497..19192)
                     /locus_tag="WGR04_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793964.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFQ"
     gene            19883..20260
                     /locus_tag="WGR04_000331"
     CDS             19883..20260
                     /locus_tag="WGR04_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0793965.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            20349..20918
                     /gene="rsmD"
                     /locus_tag="WGR04_000332"
     CDS             20349..20918
                     /gene="rsmD"
                     /locus_tag="WGR04_000332"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ0793966.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVFSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PNIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            21041..21292
                     /locus_tag="WGR04_000333"
     CDS             21041..21292
                     /locus_tag="WGR04_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ0793967.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDQEHPETHDELMAKYEKIIQFK"
     gene            21389..21472
                     /locus_tag="WGR04_000334"
     tRNA            21389..21472
                     /locus_tag="WGR04_000334"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21423..21425,aa:Tyr,seq:gta)
     gene            21503..21576
                     /locus_tag="WGR04_000335"
     tRNA            21503..21576
                     /locus_tag="WGR04_000335"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21535..21537,aa:Gly,seq:tcc)
     gene            21586..21660
                     /locus_tag="WGR04_000336"
     tRNA            21586..21660
                     /locus_tag="WGR04_000336"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21618..21620,aa:Thr,seq:ggt)
     gene            21709..22893
                     /gene="tuf"
                     /locus_tag="WGR04_000337"
     CDS             21709..22893
                     /gene="tuf"
                     /locus_tag="WGR04_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0793968.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSS
                     YDFPGDDCPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVG
                     VLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFR
                     TTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSV
                     IA"
     gene            22900..22975
                     /locus_tag="WGR04_000338"
     tRNA            22900..22975
                     /locus_tag="WGR04_000338"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22933..22935,aa:Trp,seq:cca)
     gene            23090..23368
                     /gene="secE"
                     /locus_tag="WGR04_000339"
     CDS             23090..23368
                     /gene="secE"
                     /locus_tag="WGR04_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ0793969.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            23370..23906
                     /gene="nusG"
                     /locus_tag="WGR04_000340"
     CDS             23370..23906
                     /gene="nusG"
                     /locus_tag="WGR04_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ0793970.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            24007..24441
                     /gene="rplK"
                     /locus_tag="WGR04_000341"
     CDS             24007..24441
                     /gene="rplK"
                     /locus_tag="WGR04_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ0793971.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            24441..25136
                     /gene="rplA"
                     /locus_tag="WGR04_000342"
     CDS             24441..25136
                     /gene="rplA"
                     /locus_tag="WGR04_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ0793972.1"
                     /translation="MAKVSKRLKALRSSVETNKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKTTRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            25366..25866
                     /gene="rplJ"
                     /locus_tag="WGR04_000343"
     CDS             25366..25866
                     /gene="rplJ"
                     /locus_tag="WGR04_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ0793973.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            25924..26295
                     /gene="rplL"
                     /locus_tag="WGR04_000344"
     CDS             25924..26295
                     /gene="rplL"
                     /locus_tag="WGR04_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ0793974.1"
                     /translation="MSITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            26486..30664
                     /gene="rpoB"
                     /locus_tag="WGR04_000345"
     CDS             26486..30664
                     /gene="rpoB"
                     /locus_tag="WGR04_000345"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ0793975.1"
                     /translation="MSYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNKLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            30821..34996
                     /gene="rpoC"
                     /locus_tag="WGR04_000346"
     CDS             30821..34996
                     /gene="rpoC"
                     /locus_tag="WGR04_000346"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ0793976.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIAQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            35287..35544
                     /locus_tag="WGR04_000347"
     CDS             35287..35544
                     /locus_tag="WGR04_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793977.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRIGMPINIG"
     gene            35541..35690
                     /locus_tag="WGR04_000348"
     CDS             35541..35690
                     /locus_tag="WGR04_000348"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0793978.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            35869..36240
                     /gene="rpsL"
                     /locus_tag="WGR04_000349"
     CDS             35869..36240
                     /gene="rpsL"
                     /locus_tag="WGR04_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ0793979.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            36358..36828
                     /gene="rpsG"
                     /locus_tag="WGR04_000350"
     CDS             36358..36828
                     /gene="rpsG"
                     /locus_tag="WGR04_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ0793980.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            36847..38952
                     /gene="fusA"
                     /locus_tag="WGR04_000351"
     CDS             36847..38952
                     /gene="fusA"
                     /locus_tag="WGR04_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ0793981.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLSNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            39040..40224
                     /gene="tuf"
                     /locus_tag="WGR04_000352"
     CDS             39040..40224
                     /gene="tuf"
                     /locus_tag="WGR04_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0793982.1"
                     /translation="MAKEKFERSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAA
                     KAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSS
                     YDFPGDDCPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVG
                     VLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFR
                     TTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGVVSSV
                     IA"
     gene            40242..40553
                     /gene="rpsJ"
                     /locus_tag="WGR04_000353"
     CDS             40242..40553
                     /gene="rpsJ"
                     /locus_tag="WGR04_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ0793983.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            40674..>40797
                     /locus_tag="WGR04_000354"
     CDS             40674..>40797
                     /locus_tag="WGR04_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0793984.1"
                     /translation="MSTFFQQTSQAMIAKHIDRFPLLKLDRVIDWQPIEQYLNRQ"
BASE COUNT        10468 a         9834 c        10865 g         9630 t
ORIGIN      
        1 ccttattttt tgttcggcgg cttgcgtggt cgggtaaaat gaaagttttg aacggttggt
       61 cggacaggaa gatgtggcgg gttttgagtg ctttgccgat aggcgtggtg ttttttgatt
      121 tgatctacgg ttttgtgttg aatgtgttgc agggtttgga tttgcagcgt gccgtgccgg
      181 attcggaagg cgtgttggcg gttacgcccg atattgcatt caacagtttg cagattgtcg
      241 ccaacggcgg tatggcggcg gtggtctgtt tcgggttggc ggttgtgttt ttgctcaacc
      301 gttcggtgcg gcggcggcag gtgttggaaa tcggggtgtt ccggatgttg gggctggtgg
      361 cggtattggc gttcagcgcg ccgtcggtgt gggagtgggc gaacgcgctg ccgctgctgc
      421 tgaagggcgc ggacgtggtc aatacgggga atgcgcgtta tgtgctgacg gctttgtgta
      481 tgccctttcc ggcggtgtcg tgcgtcatcg ggctggtggg gcggttcagg cttcagacgg
      541 catcgggcag ggcggcaaag gcagggggtg cggtcaaggc gggcggatag ggcgcatttt
      601 tcagcgggtg cgtcgagaag cagccgatgt gtttggcagc cgcagcttgg gggggtgtag
      661 tgctaatggc ggtttctttg cttttatagt ggattaacaa aaaccagtac ggcgttgcct
      721 cgccttagcc caaagagaac gattctctaa ggtgctcaag caccaagtga atcggttccg
      781 tactatttgt actgtctgcg gcttcgtcgc cttgtcctga ttactatata aaataaatgg
      841 gcaaaaatcg gtttattatc gtttttgccg catttggatt tgttctaccg taaaacgtgt
      901 ttgacgaacg ggattcttat taaaaaaaca tctgatttct aacaaaatca gtattttttg
      961 gcacgatggc taaaattttt ccttccattt cgccatcacg tgttttccat gcgctcaaga
     1021 attgtgattt gctcattgag acgtgcccca gcgatggatc agccagcaaa acagtttctc
     1081 cgttaatacc gttcaatacc gaaaaatggt tgtttttacg gtattttaaa tacacaatta
     1141 caggaatttt tagttgtacc aactgttcaa atggcaaagc ataaccttgt gcttcaaaac
     1201 ccagttcggg cattatgcgt tgcatatcat caaaagaagc acgcatttgg gttttatcca
     1261 ttttgtctaa gatttccgct tcagaataat gtctgccata aaaattattc agtaacgtgg
     1321 caatcgaagc cgcgccgcaa gaaaaatcca aatcttgttt tactatgccg gaatctcgcc
     1381 gtgctttcca actccgtaca tggatgtttt ggtaagaagc ggggggtcaa caaacatagg
     1441 ccaagcaaaa actatatttg gggcgaaacc aatcaaagcc gcataattta tcaatttata
     1501 aagatttttt atcataatat gtatacgcgg aataaacgca tgaaacataa aaaaataaaa
     1561 tcatatgcaa ttttattgca tgaataaata tgaataaaat agataatgat gggagtaaat
     1621 acgccatgta ttttggaagt ttaaatttat taataataaa ataatttatc gtagcgcaaa
     1681 taaatcccaa aattggaacg attagaaaaa aaattaatac acccatcatc atgcaaactc
     1741 tatattaata aatagctaac taattctatt gtggaaatta gttagctaac taaaagttat
     1801 taatgattat tttcgagaat tgactgcatt gttggcagca ttggcaccaa aacctagtgc
     1861 atgaataccc ggtctccatg ccaaattccc agccaatccg cctccggcag cagcagccag
     1921 ccctgttgcc gctactcctg ttgccgcacc gattcctgcc gcagtagccg cgccttgcgc
     1981 agttcctaat ttaccatgat tatacaaatt agcaccatga tacccccatg cacctaatgc
     2041 accgccaaaa gcagcggctg caataatggg aacaaattca ccttgtgttt ctttcatttc
     2101 agcctgtgat aattgaattg ctttcacatt ttggctgtca aaaacttggc tgtctaaatt
     2161 ttgcgccatt acaggtgtaa tcatcatagc cattacagtt gcaattttcg ttgcgctggt
     2221 ttgcacataa ataggattag caaattcgct ttgattgcgt tcagtgttga tgtagctaat
     2281 actgctttct agtttgaatt tacccttgtc agtcaataat tcttccaaac ttaaaggtaa
     2341 gtcagcataa gcaatttggc tggcaacagt caaaataaaa tctattagac atttgtgttt
     2401 ttgcatcatt tcgtttgatt ttctagtttt tgagaatgat acaaagtttt ttacaaagta
     2461 aagagtcact ctgaaaaaca ttttttcatt ataaatcaaa atattgatag aataaatggc
     2521 gagcatcgat tcacggtgca ctttagtgca aagcgtacaa cgcgagcctg aaccaccagc
     2581 cgcaacagga aaagaaagcc gatagagtgc aatgcttgcc aacgtgcaag cgagcttgca
     2641 agaacgcttg gctcaacgag agcaggcaag acagaaagca gaaagcagga taggagcggt
     2701 aacgcaaagg tctcgggctt tgatttcgcc gtaaaccctg ctgccgcctt gtccggaaag
     2761 ggtgcaggcg gcgagtgccg acagggtgca gatggggagg gggggttcat ttggggtcgc
     2821 aacggaagtg gtatgcgcag atttcaaaac cgtttttgaa atacaggcgg tgcgcgtcgg
     2881 cacggtcgtg gttgacgtgg acgttgaggt ggattttggt tacccttgtt tccgcgccga
     2941 ttttgcggac ttcttccaaa aggcgcgagg cgtagccttt gcggcggctt tgcggcaggg
     3001 taacgatgtc gtcgatatgg atatgtcggc cgccggcgag ggtgcaggct tcgcggaagc
     3061 cgcagacggc gacggcgttg tgtttgcctt cttcaaaaat acccagcagg cggtagcctt
     3121 gggggcgttg gactttgttg atctgttcgg taaagcggtt gatgtcggtc agggcggaac
     3181 gcaaaacgct caaggctgca aaggcggtgg cggtgtcgtc cgcgccgatt tcgcgcaaaa
     3241 cgtaggatgc gcccgaggcg gtctgttcct gtgctttctc ggcggcgtgt ttttcttcga
     3301 ttgcctgtgc cagcatgacg tgttcgtcgg cagggttgtt ttgtccgccc tgttcgcgtt
     3361 cttcgagcag ggctttgcag tcgatgacgc gcaggtcgtt gtcggcggca aagtccatca
     3421 ggaagcggaa catttgggga ttgtcggttt ccagtttttt atcgacggcg acgcagcgga
     3481 tgttgtccac cagtatgggg cgcacccatt tcgagtagga aagcctgtgg tctttggtga
     3541 acgaggacag aatgccgcac aggcgttccg cccagtcgct gggacggaaa atcttgccgg
     3601 aactcgttgt gccgtggatg acgacttcgt aggggttgca gactaacatg gcggcttcct
     3661 gaaaagaaat gtctggctcg attatacctt atgcttatgc aggcgtgttt ggatatgccg
     3721 tctgaaaagt acgggattcg tgcggtaaaa ctttgcggcg gcaaatgtgc gataatacgc
     3781 gccgtattgc cgcttttgcg aagctgttcc gcaaacatac gggcggcgtg gacgtataac
     3841 cggatacccg cctgacgcgg gttttttacg gaagggggca aaaatgccta atccgcttta
     3901 cagacagcac atcatctcga tttcggattt gtcgcgcgaa cagttggaat gcctgcttca
     3961 gacggcattg aagctgaagg cgcatccgcg cggcgatttg ttggaaggca aacttatcgg
     4021 ttcgtgtttc tttgaaccgt ccacgcgcac gaggctgtcg tttgaaacgg cggtgcagcg
     4081 gctgggcggc aaggtcatcg gcttttcgga cggcgcgaat accagtgcca aaaaaggcga
     4141 gacgcttgcc gataccgccc gcatcatttc cggctacacc gacgcaatca tccaacgcca
     4201 ccccaaagac ggcgcggcgc gcgtggcagc ggagttttcg cgcgtccccg tcatcaacgc
     4261 cggcgacggc accaaccagc accccagcca gaccttgttg gatctggtaa cgatttatga
     4321 aacgcagggg cgtttggaca agctcaaaat cgccatggcg ggcgacttga aatacgggcg
     4381 caccgtgcat tcgctttgtc aggcgttgaa acgctggggc tgcgaatttg cctttgtttc
     4441 gccgcccagc ctcgcgatgc ccgactacat taccgaagag ttggacgaag ccggctgccg
     4501 ataccgtatc ctcggcagtt tggaagaggc ggcggaatgg gcggatatcc tgtatatgac
     4561 ccgcgtccag cgcgaacgtt tcgatgaaca ggaatttgcc aaaatccaag gcaaattcaa
     4621 cctcgaagcg tctatgctcg cccacgccaa accgaacctg cgcgtgctgc accctttgcc
     4681 gcgcgtggac gaaatccatc ccgatgtcga tgccacgccg catgcctatt atttcgagca
     4741 ggcaaccaac ggcgtttatg cgcgtatggc gatattgtcg ctggtgttga acgaagaagt
     4801 gtgaggaacc gatatggaaa ccccgaaact cagtgtcgaa gccattgaaa aaggtacggt
     4861 tatcgaccat attcccgccg gcagggggct gaccatcctg cgccagttca aacttttgca
     4921 ctacggcaac gcggtaaccg tgggcttcaa cctgcccagc aaaacccaag gcagcaaaga
     4981 catcatcaaa atcaaaggcg tgtgcttgga cgacaaagcc gccgatcgcc ttgccctgtt
     5041 cgcccccgaa gcggtggtca acaccatcga ccatttcaag gtcgtgcaga agcggcattt
     5101 gaacctgccc gatgaaattg ccgaagtgtt ccgctgtccg aacacgaatt gcgccggcca
     5161 cggcgagccg gtcaaaagcc ggttttatgt taaaaaacac aacgggcaga cgcggctgaa
     5221 atgccattac tgcgaaaaaa cctacagccg ggattcggtg gcggaagcct gacggattcc
     5281 cttaaaccga gcgggcggca tttcgtctgc cgcttgtttt gccaatctga aatggaatga
     5341 tgatgcacgc ttctgtccaa agccgtttcg caccgatact ttatgttttg attttctttg
     5401 ccggtttttt gaccgcgcaa atttggttca atcagaaagc ctatactgaa gagctgcctc
     5461 cgcttctgtc cgcattgtcc gccgtcgcgc tggtgtggct ggcgtgggcg ttcgtgtcgg
     5521 cgcgttcaaa ggctaaggcg gaaaagttct accgcgaaaa aatgatacag aacgaaagca
     5581 tacaccccgt cctgcacgct tctttgcaac acttggaaca caagccgcaa atgctcgccc
     5641 tgctggtcag aaaccacggc aaagggatgg cggaacaggt caggttcaag gcagaagtgc
     5701 tgcctgacga tgaagacgcg cgcacgattg ccgccgagtt ggcaaaaatg gatatgttcg
     5761 cattggggac ggacgcggtc gcctcgggcg aaacctatgg acgcgtgttc gccgatattt
     5821 tcgagttgtc ggcggctttg gaagggcgcg cgttcaaagg aatgttgaaa ctgacggcgg
     5881 aatataaaaa catcttcggc gatgcctgca gttcggaaac ggcgttggag ttgggcgcgc
     5941 tcaatcaggc gttgcaggag atttcaaaaa catcggaaaa gtccaaacgg atattttatt
     6001 gaagatggaa aaatgccgtc tgaaacggaa ggtgtttcag gcggcatttt tgtcggatga
     6061 ttaattattc ggagcggttg aagccaaact tcacgcggct gcgaccctga tccggtatat
     6121 tgtccaaatc gcgtcccgga ttggcggcgg tgtcgcctac ggaaatatcg gagatgtttt
     6181 ccaaaatgat ggcggacgac aggtgttcgg aggtgcggta aaccattgcc aagcccactt
     6241 cttcggcagg agtggaaatc agctcgacgg tatccctgct tttgaaattg ttggagaggt
     6301 cgacctgcat cgttttcttg cgtttgtaga ggctcaaaac cgtgcctttg tccaaaccgt
     6361 ccgcctcgcc tttgtcgatg gtgatggttt gaaactggcc ggcaatcctt gtaccttcaa
     6421 acacggaaac gattttagcc tgaaccgggc gggacggttc gtgcggcatc atgttgaagc
     6481 ggtcggtgtc ttccggcatt ttcatcaggt agtcgccctg ctgtatttcg gaaatggcgg
     6541 tttcgaccac cagcggctgt atcgaagggg tgcgcagcgg ggtaatcaaa ggatgggtgc
     6601 gggtatggta ttcgttgtct ttcggccgtt ctccagcctg tttcgagcgt tgttcgagga
     6661 cagagtcggt atagtcgagg gagcgcacga tgccgctgaa tgcgacttcc tgcccgagga
     6721 atttacccgt atccggatcg gtgatgtttt tattgattcg gtaggtcagg tagcggcccg
     6781 gctctttcag gcctttggtg taaaccctgg tgcctttggt gtacagcagc ctgccttccg
     6841 ggcccgagag caggcgcggc gcggcagcgg tttctttgcg ggaaacgatt tgcgggtgcc
     6901 gcataaagat gcggtagaag ttgacatcga tggcgggaat accgtatccg gacacttcct
     6961 tatccggact cattttgacg acggggatgc cgtctgtctg ttccaagccg aggcgcggtt
     7021 cgccgtcaac gtggcgcaac accaatacct ggtccggata aatcaggtcg ggattgtgga
     7081 tttgatcccg gttcgcgtcc cacaggcggc cccattgcca cgggctgtac aggtatttgc
     7141 ccgaaatgcc ccacagggtg tcgccctgtt tgaccgtgta gcgttccggc gcgttcgggc
     7201 gcacctccaa atttgccgcc aaagtttgtg ttgagaatgc catacctgcc gcgcagagca
     7261 gggttataat acgacgttgc ataaccgttc cccttatctg ataaatttcg gtttgtcttg
     7321 cttgattggg ttggaaaaag cggcggcagc ccctcgggat gtgccgcgtg ataaaaaatg
     7381 ttccgcattt taacatcgaa ttatccgcac catcacggta attatgaaaa acaggcggcg
     7441 tatccgccga aggaaagaga aaattatggc tttattgaat atcttgcaat atcccgacga
     7501 gcgtctgcac acggtggcaa agcctgtcga acaagtcgac gagcgcatcc ggaagctgat
     7561 tgccgatatg tttgaaacga tgtacgaatc gcgcggcatc gggctggcgg cgacgcaggt
     7621 cgatgtgcac gagcgcgtgg tcgtgatgga tttgaccgaa gaccgcagcg aaccgcgcgt
     7681 gttcatcaac cccgtcatcg ttgaaaaaga cggcgaaacc acttacgaag agggctgcct
     7741 gtccgtgccg ggcatttacg acaccgtaac ccgcgccgaa cgcgtcaagg tcgaggcttt
     7801 gaacgaaaaa ggcgaaaagt tcacgctgga ggcggacggc ttgctggcga tttgcgtgca
     7861 gcacgagttg gaccacctga tgggtatcgt gtttgtcgaa cgcctttccc aactcaagca
     7921 ggggcggatt aagaccaagc tgaaaaaacg tcagaaacat acgatttgac ccttttgccg
     7981 tgccgtctga acgctgcaaa gttttcagac ggcacggtct tgtccgacaa ttttacgcac
     8041 acgcaggaac acgctatgaa agtcatcttc gccggcacgc ccgattttgc cgccgccgcc
     8101 ttaagagccg ttgccgccgc cggttttgaa atcccgctgg tgctgaccca gcccgaccgt
     8161 ccgaaagggc gcggtatgca actgactgcc ccgcccgtca aacaagccgc gctggaactc
     8221 ggtttgcgcg tcgaacagcc cgaaaaactg cgtaacaacg ccgaagccct gcaaatgctc
     8281 aaagaggtcg aggcagacgt aatggtggtt gccgcctacg gtttgattct gccgcaagac
     8341 gtgttggatg tgccgaaaca cggctgcctc aacatccacg cttcgctgct gccccgttgg
     8401 cgcggcgcgg cgccgattca gcgcgcgatt gaggccggcg atgccgaaac cggcgtgtgc
     8461 attatgcaga tggatgccgg tttggacacc ggtgatgtgg tcagcgaaca ccgctacgcc
     8521 atccaaccga ccgataccgc caacgaagtc cacgacgcgc tgatggaaat cggtgcggcg
     8581 gcggttgttg ccgatttgca acagcttcaa agcaaaggcc gtctgaacgc ggtcaaacag
     8641 cccgaagaag gtgttactta cgcgcaaaaa ttgcgcaaag aagaggcgcg tatcgattgg
     8701 agcgaaagcg cggcggttat cgaacgcaaa atccgcgctt tcaaccccgt gcctgccgcg
     8761 tgggttgagt atcagggcaa gccgatgaaa atccggcggg cggaagtggt ggcgcaacaa
     8821 ggcgcggcag gcgaagtgtt gtcctgttcg gcggacggtt tggtcgttgc ctgcggcgaa
     8881 aacgcgctga agattaccga attgcagcct gccggcggca ggcgtatgaa tatcgcggcg
     8941 tttgcagcag gacggcatat cgaagcaggg acgaagctgt aaatcccttc agacggcatt
     9001 ccgatccgca aacgggaatg ccgcctgaaa ccatcagttg aagaaagcga atcacataat
     9061 atgagtatgg cacttgccca aaaacttgcc gccgacagca ttgcggcggt tgccgaagga
     9121 cgcaaccttc aggacgtgtt ggcgcaaatc cgcaccgcgc atcccgacct tacggcacag
     9181 gaaaacggcg cgttgcagga catcgcctac ggctgccagc gttatttggg cagtttgaaa
     9241 catatgctcg cgcagatgct gaaaaagccg attgacaacc cgcagctcga aagcctgctt
     9301 ttggcggcgt tgtaccaact gcattacacg cgcaacgcgc cgcacgccgt ggtcaacgaa
     9361 gcggtggaaa gcatagccaa aatcggacgc gggcagtacc gttcgtttgc caacgcgatt
     9421 ttgcgccgct ttttgcgcga acgcgacaag cttgcggctt cctgtaaaaa agacgatgtg
     9481 gcgaaacaca acctgccgct gtggtgggtg gcttacctga aaaaccatta tccgaaacac
     9541 tggcacaaca tcaccgccgc gctacaatcc catccgccga tgacgttgcg cgtcaaccgc
     9601 cgacacggca atgccgaaag ctatttggaa aaactggctg cggaaggtat tccggccagg
     9661 gcgttggacg aatatgcggt tacgttggaa gaagccgtgc cggtaaaccg tctgcccggt
     9721 ttttcagacg gcattgtttc ggtacaggac ttcggtgcgc agcaggcggc gtatcttctt
     9781 aacccgaaag acggcgaacg gattttggac gcgtgcgccg cgccgggcgg caagacgggg
     9841 catatcttgg aactggcgga ttgccgcgtt accgccttgg acatcgatgc aggccgtctg
     9901 aagcgtgtgg aggacaatat cgcccgtttg ggctttcaga cggcatcggc ggcgtgtgcc
     9961 gatgcgcggg atttggcggc gtggtatgat ggaaagcctt tcgacgcaat ccttgccgac
    10021 gtgccatgca ccgcttccgg cgtggcgcgg cgcaatcccg acgtgaaatg gctgcgccgt
    10081 ccgaccgacg cgctcaaaac cgcccgccag caggaagccc tgctggacgc actttggcag
    10141 acgctgaaac agggcggcag aatgctgctt gccacctgtt ccgtgttcgt cgaagaaaac
    10201 gacgggcaat tgcaaaaatt cctcaaccgc catgccgatg ccgaaccgat cgaatcgcgg
    10261 gtgcttttac cgaacaaaca ccaagatggc ttttattacg cgcttattca aaagcattaa
    10321 acaatggctt gtgctgctgc cgatgctttc cgttttgccg gacgcggcgg cggaggggat
    10381 agatgtgagc cgcgccgaag cgaggataac cgacggcggg cagctttcca tcagcagccg
    10441 cttccaaacc gagctgcccg accagctcca acaggcgttg cgccggggcg tgccgctcaa
    10501 ctttacctta agctggcagc tttccgcccc gataatcgct tcttatcggt ttaaattggg
    10561 gcaactgatt ggcgatgacg acaatattga ctacaaactg agtttccatc cgctgaccaa
    10621 ccgctaccgc gttaccgtcg gcgcgttttc gacagactac gacaccttgg atgcggcatt
    10681 gcgcgcgacc ggcgcggttg ccaactggaa agtcctgaac aaaggcgcgc tgtccggtgc
    10741 ggaagcaggg gaaaccaagg cggaaatccg cctgacgctg tccacttcaa aactgcccaa
    10801 gccatttcaa atcaatgcat tgacttctca aaactggcat ttggattcgg gttggaaacc
    10861 tctaaacatc atcgggaaca aataatgcgc cgttttctac cgatcgcagc catatgcgcc
    10921 gtcgtcctgt tgtacggact gacggcggca accggcagca ccagttcgct ggcggattat
    10981 ttctggtgga ttgttgcgtt cagcgcaatg ctgctgctgg tgttgtccgc cgttttggca
    11041 cgttatgtca tattgctgtt gaaagacagg cgcgacggcg tattcggttc gcagattgcc
    11101 aaacgccttt ccgggatgtt tacgctggtt gccgtactgc ccggcgtgtt tctgttcggc
    11161 gtttccgcac agttcatcaa cggcacgatt aattcgtggt tcggcaacga tacccacgag
    11221 gcgcttgaac gcagcctcaa tttgagcaag tccgcattga atctggcggc agacaacgcc
    11281 ctcggcaacg ccgtccccgt gcagatagac ctcatcggcg cggcttccct gcccggggat
    11341 atgggcaggg tgctggaaca ttacgccggc agcggttttg cccagcttgc cctgtacaat
    11401 gccgcaagcg gcaaaatcga aaaaagcatc aacccgcaca agctcgatca gccgtttcca
    11461 ggtaaggcgc gttgggaaaa aatccaacgg gcgggttcgg tcagggattt ggaaagcata
    11521 ggcggcgtat tgtacgcgca gggctggctg tcggcgggta cgcacaacgg gcgcgattac
    11581 gccttgtttt tccgtcagcc ggttcccaaa ggcgtggcag aggatgccgt cttaatcgaa
    11641 aaggcaaggg cgaaatatgc tgagttgagt tacagcaaaa aaggtttgca gacctttttc
    11701 ctggcaaccc tgctgattgc ctcgctgctg tcgatttttc ttgcactggt catggcactg
    11761 tatttcgccc gccgtttcgt cgaacccgtc ctatcgcttg ccgagggggc gaaggcggtg
    11821 gcgcaaggcg atttcagcca aacccgtcct gttttacgca atgacgagtt cgggcgtttg
    11881 accaagttgt tcaaccacat gaccgagcag ctttccatcg ccaaagaagc agacgagcgc
    11941 aaccgccggc gcgaggaagc cgccagacat tatcttgaat gcgtgttgga ggggctgacc
    12001 acgggcgtgg tggtgtttga cgaacaaggc tgtctgaaaa ccttcaacaa agcggcggaa
    12061 cagattttgg ggatgccgct tacccccctg tggggcagca gccggcacgg ttggcacggc
    12121 gtttcggcgc agcagtccct gcttgccgaa gtgtttgccg ccatcggcgc agcggcaggt
    12181 acggacaaac cggtccatgt gaaatatgcc gcgccggacg atgccaaaat cctgctgggc
    12241 aaggcaaccg tcctgcccga agacaacggc aacggcgtgg taatggtgat tgacgacatc
    12301 accgttttga tacacgcgca aaaagaagcc gcgtggggcg aagtggcaaa acggctggca
    12361 cacgaaatcc gcaatccgct cacgcccatc cagctttccg ccgaacgtct ggcgtggaaa
    12421 ttgggcggga agctggatga gcaggatgca caaatcctga cgcgttcgac cgacaccatc
    12481 gtcaaacagg tggcggcatt gaaggaaatg gtcgaagcat tccgcaatta tgcgcgttcc
    12541 ccttcgctca aattggaaaa tcaggatttg aacgccttaa tcggcgatgt gttggcattg
    12601 tatgaagccg gtccgtgccg gtttgcggcg gagcttgccg gcgaaccgct gatggtggcg
    12661 gcggatacga ccgccatgcg gcaggtgctg cacaatattt tcaaaaatgc cgccgaagcg
    12721 gcggaagaag ccgatgtacc cgaagtcagg gtaaaatcgg aagtggggcg ggacggtcgg
    12781 attgtcctga cggtttgcga caacggcaaa gggttcggca gggaaatgct gcacaacgcc
    12841 ttcgagccgt atgtaacgga caaaccggcg ggaacgggat tgggtctgcc cgtggtgaaa
    12901 aaaatcattg aagaacacgg cggccgcatc agcctgagca atcaggatgc gggtggggcg
    12961 tgtgtcagaa tcatcttgcc aaaaacggta aaaacttatg cgtagcagcg atattttaat
    13021 tgtagacgac gaaatcggca tccgcgacct gctgtcggaa atcctgcagg acgaaggtta
    13081 ttcggtcgca ttggcggaaa acgccgaaga ggcgcgcaag ctgcgccatc aggcgcgtcc
    13141 ggcgatggtg ctgctggata tttggatgcc cgattgcgac ggcatcaccc ttttgaagga
    13201 gtgggcgaaa aacgggcagc ttaatatgcc ggtcgtcatg atgagcgggc atgccagcat
    13261 tgacacagca gtggaagcca ccaaaatcgg tgcgctcgac tttttggaaa aaccgatctc
    13321 cctgcaaaaa ctgctgtctg ccgtcgaaaa cgcgttgaag tacggtgcgg cgcaaaccga
    13381 aacggggcct gtattcgaca agctgggcaa cagtgcggcg attcaggaga tgaaccgcga
    13441 agtcgaagcg gcggcaaagc gcacgtcccc tgtatttttg acgggtgagg tgggttcgcc
    13501 gtttgaaacc gtagcgcgct atttccataa aaacggtacg ccgtgggtca gtccggcaag
    13561 ggtcgaatat ctgatcgata tgccgacgga actgttgcag aaagctgaag gcggtgtttt
    13621 atatgtgggc gatattgccc aatacagccg caacatccaa accggcattg cctttatcat
    13681 cggcaaggca gaacgctgcc gcgtcagggt ggtcgcatcg ggcagcaggg cggcaggttc
    13741 agacggcatt gcctgcgagg aaaagctggc ggaactgctg tcggaatcgg tcgtccgtat
    13801 tccgccgctg cgtatgcagc atgaagatat tcccttcctg atacagggga ttgcctgcaa
    13861 tgtggcggaa agtcaaaaga ttgcgcccgc cgcattcagt gaagaggcac ttgccgcatt
    13921 gacccgttac gactggccgg gaaatttcga ccaactgcaa agcgtcgttg caacgctgtt
    13981 gttggaggcg gacggacagg aaatcggcgc ggcggcggtt tcttcccttt tggggcagaa
    14041 tgtgcctgcc gagggagcag aagatatggt gggcgggttt aatttcaacc tgcccctgcg
    14101 cgaattgagg gaggaggtgg agcggcgtta tttcgagtac cacatcgccc aagaaggtca
    14161 gaatatgagc aaagtggcgc agaaagtcgg tttggaacgc acgcaccttt accgcaaact
    14221 caaacagctc ggcatcggcg tttcgcgccg ggcgggggaa aaaaccgaag aataggcccg
    14281 gacggccggt tttccggctg cgggcttttg ttttcagacg gcatttggtg caaatgccgt
    14341 ctgaaatcgt aaggggacgg attttatgac agaggacgaa cgtttcgcgt ggctgcaatt
    14401 ggcgtttacg ccctatatcg gcgcggaaag tttcctgctg ctgatgcgcc gtttcggcag
    14461 cgcgcaaaat gccctgtccg caccggcgga acaggtggcg gcactgatac ggcacaaaca
    14521 ggcgcttgag gcttggcgca atgcggaaaa acgcgctctg gcgcggcagg cggcagaagc
    14581 ggcattggaa tgggaaatgc gggacggatg ccgcctgatg ctgcttcagg atgaagattt
    14641 ccccgaaatg ctgactcagg gtataactgc gccgccggtt ttgtttttgc gcggcaacgt
    14701 acaactgctg cacaaacctt ccgccgccat tgtcggcagc cgtcatgcca cgccgcaggc
    14761 aatgcggatt gccaaagatt tcggcaagtc gttgggcggg caaaatatcc ccgttgtgtc
    14821 gggtatggct tccggtatcg atactgccgc ccatcagggc gcactggagg cagaaggcgg
    14881 caccatcgcc gtgtggggga cgggtataga ccgcatttac ccgccgtcca ataaaaacct
    14941 tgcctatgaa atcgccgaaa aaggattgat tgtcagcgag tttcccatcg gtacgcgccc
    15001 gtatgccggc aattttccgc gccgcaaccg cctgattgcc gccctgtcgc aagtaacgct
    15061 ggtggttgaa gccgccttgg aatccggttc gctgattacc gccggcttgg cggcagaaat
    15121 ggggcgcgaa gtgatggcgg tacccggttc gatagacaat ccgcacagca aaggctgcca
    15181 caaactgatt aaagacggcg caaaattggt ggaatgcctg gacgacatcc tgaacgaatg
    15241 cccggggcta ttgcaaaata cgggtgcttc atcatattct ataaataagg gaatacctga
    15301 aaagcgcatc actgccgttc agacggcatc cgaccagctg tctctgcctg aaggcaaaat
    15361 gccgtctgaa aagacggaga accgacccgt cggcggcagt atcttggaca ggatgggttt
    15421 cgacccagtt catcccgacg tgcttgccgg acagttggct atgcctgccg cagatttgta
    15481 tgccgcactg ttggaattgg aattggacgg cagcgttgcc gcaatgcccg gcggcagata
    15541 ccagcgtatc cgaacttgaa cgcactttat attaaggaac acgaatgacc gaagtcatcg
    15601 cctacctcat cgaacatttc caagatttcg atacctgtcc gccgcccgaa gacttgggta
    15661 tgctgcttga agaagcgggt ttcgatacga tggaaatcgg caacaccctg atgatgatgg
    15721 aagtattgct caacagctcc gaattttccg ccgaacctgc cgacagcggc gcattgcgcg
    15781 tgtacagcaa agaagaaacc gacaacctgc cgcaggaagt gatggggctg atgcagtatc
    15841 tgattgaaga aaaagccgtc agctgcgaac agcgggaaat catcatccac gcgctcatgc
    15901 acattccggg cgacgaaatt accgtagata ccgccaaagt gctgaccctg ctgcttttat
    15961 gggcaaacaa gagcgagctg cccgtgttgg tcggcgacga gctgatgagc gcgcttttac
    16021 tcgacaacaa acccacgatg aactgaagcg gcttcagacg gcccgcccga ggccgtctga
    16081 aacgtcggca tcaaaaccac catccagaga acgacaaatg gcgaaaaacc tattaatcgt
    16141 cgaatccccg tccaaagcca aaaccctgaa aaaatatttg ggcggcgatt ttgaaatcct
    16201 tgcatcctac ggacacgtcc gcgacctcgt ccccaaaagc ggcgcggtcg atcccgacaa
    16261 cggctttgcg atgaaatacc aactcatcag ccgcaacggc aaacacgtcg atgccatcgt
    16321 cgccggtgcc aaagaagctg aaaacatcta cctcgccacc gacccggata gggaaggcga
    16381 agccatttcc tggcatcttt tggaaatcct caaatccaaa cgcggcttga aaaacatcaa
    16441 gccgcagcgt gtcgtgttcc acgaaatcac caaaaacgcc gtgctcgatg ccgttgccca
    16501 tccgcgcgaa atcgaaatgg acttggtcga tgcgcaacaa gcccgtcgcg ctttggacta
    16561 tttggtcggc tttaaccttt cgccattgtt atggaaaaaa atccgccgcg gtttgagcgc
    16621 gggtcgtgtg caaagccctg ctttgcgcct gatttgcgaa cgcgaaaacg aaatccgtgc
    16681 gtttgaagcg caggaatatt ggacggtaca tctcgacagc cacaaaggcc gcagcaagtt
    16741 caccgccaaa ctcgcccaat acaacggcgc gaaactcgaa caattcgacc tgccgaatga
    16801 agccgcgcaa gccgacgtgt tgaaagaact cgaaggcaaa gaggccgtcg ttaccgccat
    16861 cgaaaagaaa aagcgcagcc gcaatcccgc cgcgccgttc accacatcca ccatgcaaca
    16921 ggatgccgta cgcaaactcg gcttcaccac cgaccgcacc atgcgtacct ctcaacagct
    16981 ttatgaaggt atagacgtag ggcagggcgc catcggtctg attacctata tgcgtaccga
    17041 cagcgtgaac ttggcagatg aagccttaac cgaaatccgc cattacatcg aaaacaaaat
    17101 cggcaaagaa tatctgccga gtgccgccaa acaatacaaa accaaatcca aaaacgccca
    17161 agaagcgcac gaagcgatcc gtccgacttc cgtgtaccgc acgcccgaaa gcgtcaaacc
    17221 cttcctgagc gcagaccagt tcaaactcta tcaaatgatt tggcagcgca ccgtcgcctg
    17281 tcagatgatg cccgccaaat tcgaccaaac caccgtcgat attaccgtcg gcaaaggcgt
    17341 gttccgcgta accggacaag tgcaaacctt cgcaggcttc ctcagcgttt acgaagaaag
    17401 cagcgacgat gaagaaggcg aagacagcaa aaaactgccc gaaatgagcg aaggcgataa
    17461 attgcccgtg gacaaactct acggcgaaca acactttacc actccgccgc cgcgctacaa
    17521 cgaagccacg ctggttaaag ccctcgaaga atacggcatc ggccgcccct cgacctacgc
    17581 cagcatcatc tccacgctca aagaccgcga atacgttacc cttgagcaaa aacgcttcat
    17641 gcccaccgac acaggcgaca tcgtcaacaa attcctgacc gaacacttcg cccaatacgt
    17701 cgattaccac ttcaccgcca aactcgaaga ccagctcgac gaaatcgccg gcggcaaacg
    17761 ccaatgggtt cccgtgatgg ataaattctg gaagccgttc atcaagcaag tcgaagaaaa
    17821 agaaggcatc gaacgcgcca aatttaccac gcaggaactt gatgaaacct gcccgaaatg
    17881 cggcgaacac aaactgcaaa tcaaattcgg caaaatgggt cgttttgttg cgtgtgccgg
    17941 ttatcccgag tgcagctaca cgcgcaacgt caacgaaact gccgaagaag ccgccgagcg
    18001 catcgccaaa gccgaagccg aacaggccga actcgacgga cgcgagtgcc cgaaatgtgg
    18061 cggtcgccta gtgtacaaat acagccgcac cggcagcaaa ttcatcggct gcgccaacta
    18121 ccccaaatgc aaacacgtcg agccgctgga aaaaccgaaa gataccggcg tccagtgtcc
    18181 gcaatgcaaa aaaggcaacc tcgtcgagcg caaatcccgc tacggcaaac tgttttacag
    18241 ttgcagcacc tatcccgact gcaactacgc cacttggaac ccgcccgttg ccgaaagctg
    18301 tccaaaatgc gcatggccgg ttttgaccat caaaaccacc aaacgctggg gtgtggaaaa
    18361 agtttgtccg caaaaagaat gcggctggaa agaacagatt gaaccgcctg caccgaaaga
    18421 gtgagattag gttggtttga aagagaaaag gtcgtctgaa aaattttcag acgacctttg
    18481 cttttctgtg attggtttat tggaatccgc gtgttgtttt aaagtccgat aaaatccggt
    18541 tcatttcagg cgcaaacaag gcgatgtaat cgtaagatag accgcgactg gcactgggat
    18601 ggggaaagca gacgacttcg caatcttcaa acgattggaa tttgacattg aaacgtgtac
    18661 cgtcaaattc tttttgcacc gtctccagcg gtttggtctg cttaccgacc aactgctcga
    18721 agcgtggcag tacattttgg ttgttcagaa aatccgccaa cctgctgccc atgaagagga
    18781 tgactttcgg acgcagtttt tcgatgtggt agagaaaatt atcgatgtgc tcgggttgtg
    18841 tgaacttgtc gggattgtcg atagtgttgc cctgtgtagc agcccagttg gtttgaacca
    18901 gggatttttc aaatgcaccg cccaatccat tttcgtctaa ggggtgtccc cacatttcaa
    18961 accaattttt tatcgtatta tcgtaacgcc acttttttgc ctgctctccg aaatagaggg
    19021 atttgtttgc aaatgtatgg tcgattttgt tttcagggag tttgtattca cctgctacat
    19081 aagcagcctc atcggcttta ctccaacccc attcatagcc acaaatcatt aagccatgtt
    19141 tgtcgttgta gcctttgaac aggctgttgc tcaaattcaa atccttcatc atgaactctt
    19201 ccttttaaaa tttaagagcg attgacttca atgtttttag atggggtgga aaaatccttg
    19261 tgtaggcaac ataaattcaa taaatttctt gataattcga aacctactaa tagcgcacct
    19321 ataaaagctt tttcattacg ttcagcatga cggtcacgtc gttcatattt tttacgcttg
    19381 ctgttccctg ttattacagc taagccaagt gatatggcga gaattgccca aacaatagta
    19441 cttaaataac aattttcccc atactatcaa taaggaaaga aaaaaccttt tagtattagg
    19501 tcgatagatt ataatccatg cccatgaaaa tgcaataaga gttatacata agatacaagc
    19561 tgacaggatt tctttatttt taattaaata acttagagcg atgaggatga caggtgtcaa
    19621 gaaaataata gttacatccc gatagctata aaagaaaact gccctatttt gaatgtggag
    19681 atgtgcacag aatccaatat agctaaggat aatagttaat ataataaaaa aagaccacca
    19741 agggtgaaga gataggaatt ccatgttttc cctttttttg taaaaaggaa aaaatcttat
    19801 ctaatagtta attgcctaac cagccagaag aagtttaaaa tctatcccaa taattcaacc
    19861 atctatacag aaagttcagc ttatggaaac ccacgaaaaa atccgcctga tgcgcgaatt
    19921 gaataaatgg tcccaggagg atatggcgga aaagctggcg atgtcggcag gagggtatgc
    19981 caaaatcgaa cggggcgaaa cgcagttgaa tatcccgcgt ttggagcagt tggctcagat
    20041 tttcaaaatc gatatgtggg acttgctcaa atcgggcggt ggtgggatgg tgtttcagat
    20101 taatgaaggt gatagtggtg gcgatattgc gttgtatgcg tcgggtgatg tttcgatgaa
    20161 aatagaattt ttaaaaatgg agttgaaaca ctgcaaagaa atgttggaac aaaaagacaa
    20221 agaaatcgag ctgctccgca agctgaccga aaccgtttaa acagatatgc cgtctgaaaa
    20281 aagttttcag acggcatatt ctttgacagg tcttgtataa taccgtttga acttacaggt
    20341 ttttgattat ggcggcaggc aaacatacca aacacagcaa ccgggtacgc attatcggcg
    20401 ggcaatgccg gggcaggaaa ttgagtttca catccgccga cggactgcgt ccgacacccg
    20461 acagcgtgcg tgaaaagctg tttaactggc tgggacagga tttgacgggt aaaacggttt
    20521 tggatctctt cggaggcagc ggcgcactcg gtatagaagc cgcttcgcgc aacgccaaac
    20581 gcgtgctgat ttcggataac aaccgccaaa ccgtgcagac cttgcagaaa aacagtcgcg
    20641 aactgggttt ggggcaggtg caaatcgtct tttcagacgg catcgcatat ttgaagaccg
    20701 tatccgaaca gtttgatgtt gtctttctcg acccgccgtt tgcatggcag gactggcaaa
    20761 tcctgtttga tgccttgaag ccgtgcctga acccccgggc attcgtctat ctcgaggcgg
    20821 gtacgctgcc gaatattccc gattggctga cggaatatag agaagggaaa tcggggcaga
    20881 gtacatttga attaagggtt ttccaagtgg ctgaataata tgcgctttga taatcatttc
    20941 cgagttgtaa acattcgttt gcaaccgtcc ggttcaaaaa aaccttgtgc tataatccgc
    21001 gcccgcccgg ttttgataat ttagtggaaa aggaaaagaa atgtcgcttt ttattaccga
    21061 cgagtgcatc aactgcgacg tatgcgaacc cgaatgcccc aatgatgcca tttcccaagg
    21121 cgaggaaatt tacgaaatca accccaacct ctgcacgcag tgcgtcggac actacgatga
    21181 gccgcagtgc cagcaggttt gcccggtgga ctgcatcctg attgaccaag aacatcccga
    21241 aacccatgac gagttgatgg cgaaatacga aaagattatc cagtttaaat aaattctttt
    21301 taaaacatca aattatgtct gttttgaaat aaaatcaaaa aaaaacttga cggaaaagca
    21361 agccgctaat aaactaacgt tctcttttgg agggattccc gagcggtcaa agggggcaga
    21421 ctgtaaatct gttgcgaaag cttcgaaggt tcgaatcctt ctccctccac caaaattctt
    21481 acttggggca gtagcgagta atgcgggtgt agctcaatgg tagagcagaa gccttccaag
    21541 cttacggtga gggttcgatt cccttcaccc gctccaaaca attaggccca tgtagctcag
    21601 gggtagagca ctcccttggt aagggagagg tcggcagttc aaatctgccc atgggcacca
    21661 tccctcgatt attcatttct ttaaggctta gatatatagg atattgccat ggctaaggaa
    21721 aaattcgaac gtagcaaacc gcacgtaaac gttggcacca tcggtcacgt tgaccatggt
    21781 aaaaccaccc tgactgccgc tttgactact attttggcta aaaaattcgg cggtgctgca
    21841 aaagcttacg accaaatcga caacgcaccc gaagaaaaag cacgcggtat taccattaac
    21901 acctcgcacg tggaatacga aaccgaaacc cgccactacg cacacgtaga ctgcccgggg
    21961 cacgccgact acgttaaaaa catgattacc ggcgccgcac aaatggacgg tgcaatcctg
    22021 gtatgttccg cagccgacgg tcctatgccg caaacccgcg aacacatcct gctggcccgc
    22081 caagtaggcg taccttacat catcgtgttc atgaacaaat gcgacatggt cgacgatgcc
    22141 gagctgttgg aactggttga aatggaaatc cgcgacctgc tgtccagcta cgacttcccc
    22201 ggcgacgact gcccgattgt acaaggttct gcactgaaag ccttggaagg cgatgccgct
    22261 tacgaagaga aaatcttcga attggctgct gcattggaca gctacatccc gactcccgag
    22321 cgtgccgtgg acaaaccttt cttgttgcct atcgaagacg tattctctat ttctggtcgt
    22381 ggtacagtag taaccggtcg tgtagagcgc ggtatcatcc acgtcggtga cgagatcgaa
    22441 atcgtcggtc tgaaagaaac tcaaaaaacc acttgtaccg gtgttgaaat gttccgcaaa
    22501 ctgctggacg aaggtcaggc gggcgacaac gtaggcgtat tgctgcgcgg taccaaacgt
    22561 gaagacgtgg aacgcggtca ggtattggcc aaaccgggta ctatcactcc tcacaccaaa
    22621 ttcaaagcag aagtatacgt actgagcaaa gaagagggcg gccgccatac cccgttcttc
    22681 gccaactacc gtccccaatt ctacttccgt accaccgacg taaccggcgc ggttactttg
    22741 gaagaaggtg tggaaatggt aatgccgggc gagaacgtaa ccattaccgt agaactgatt
    22801 gcgcctatcg ctatggaaga aggcctgcgc tttgcgattc gcgaaggcgg tcgtaccgtg
    22861 ggtgccggcg tggtttcttc tgttatcgct taagtttaga ggccaatagc tcaattggta
    22921 gagtatcggt ctccaaaacc gagggttggg ggttcgagac cctcttggcc tgccaaataa
    22981 aaaattaacc ggccttgtgc cggttaattt ttttgtattt gttatttagt aaactctctt
    23041 gccatttaca tggattgaga atagacagat gctatgatgg ataaataata tgacagaaca
    23101 tacgcctgaa aaaaagaacg ttaaagtgga tcaactggtt gttcaagata aagaatctgc
    23161 atctaattcc ggtaaggaag ggttttttgc atatttctca aattcttggt ccgaattcaa
    23221 aaaggtggtt tggcctaagc gtgaagatgc tgtcagaatg actgtatttg ttatagtgtt
    23281 tgttgctgtg ctttctatat ttatctatgc ggcagataca gcaatttcgt ggttattttt
    23341 tgatgtattg ctgagaaggg aaggttgaga tgtcgaaaaa atggtatgtt gtacaggcgt
    23401 attcggggtt tgagaagaat gtccaacgaa tattggaaga gcgcattgcc cgtgaggaga
    23461 tgggagatta tttcggacaa attctggtgc ctgtagagaa agttgttgat atccgcaatg
    23521 gtcgtaagac tattagtgaa agaaagtcat atcctggtta tgtgctagtt gagatggaaa
    23581 tgacagatga ctcttggcat cttgtaaaaa gcaccccccg tgtttccggt tttattggag
    23641 ggagggctaa tagacctacg ccgattagtc agagagaggc tgaaattatt ttacagcagg
    23701 ttcagaccgg catagagaag ccgaaaccaa aagttgaatt tgaggtcggt caacaggttc
    23761 gtgtaaatga agggccgttt gcggatttta acggggtggt tgaggaggtc aattatgaac
    23821 ggaataagtt acgcgtgtct gttcagatat ttggtagaga aacacccgtt gagctggagt
    23881 tcagccaggt tgaaaagatt aactgatttt tatacttgaa aaaaaagcaa taagaggata
    23941 gaatcaaaaa ttaacttggg gagcggaaat ggttccgcgt cttacccgtt tttaggagtc
    24001 cgttaagtgg caaagaaaat tatcggctat attaaactgc aaattcctgc aggtaaagcc
    24061 aatccatctc ctccggttgg tcctgctttg ggtcagcgcg gtttgaatat tatggaattt
    24121 tgtaaggcat ttaatgctgc aacccaaggt atggagcctg gcttaccgat tccggttgtg
    24181 attactgcat ttgcagataa atcattcaca tttgtgatga aaaccccgcc agcttctatc
    24241 ttgttgaaaa aggctgccgg tttgcaaaaa ggtagttcta atcctctgac caacaaagtg
    24301 ggtaaattga cccgtgccca gttggaagaa attgctaaaa ctaaagagcc tgatttgact
    24361 gctgctgact tggatgcggc tgttcgtact atagcaggtt ctgctcgctc aatgggcttg
    24421 gacgtggagg gtgttgtata atggccaaag tatctaaacg cttgaaagct cttcgctctt
    24481 ctgttgaaac taacaaactg tacgcaatcg acgaagcaat tgctttggtt aagaaagctg
    24541 ctaccgctaa atttgacgag tctgttgacg tatctttcaa cttgggtgtt gatccgcgta
    24601 aatctgacca agttatccgt ggttcagtcg ttctgcctaa aggtaccggt aagacaaccc
    24661 gtgtggctgt atttactcaa ggtgcaaatg cagaagctgc taaagaagct ggtgcagata
    24721 tcgtcggttt cgaagatttg gctgctgaaa tcaaagcagg caatctgaac tttgacgttg
    24781 ttattgcttc tcccgatgca atgcgtattg ttggtcaatt gggtactatt ttgggtcctc
    24841 gaggcttgat gccaaaccct aaagtaggta ctgttactcc taacgttgct gaagcagtaa
    24901 aaaatgcaaa agcaggtcaa gtgcagtacc gtaccgacaa ggcaggtatt gttcatgcaa
    24961 cgattggtcg tgcttcattc gctgaagctg atttgaaaga gaactttgat gcgttgctgg
    25021 atgctatcgt taaagccaag cctgctgccg ctaaaggtca gtatctgaaa aaagttgctg
    25081 tgtctagcac catgggtttg ggtattcgcg ttgatacatc aagcgtaaat aactaatctt
    25141 aaggaatgtt caagcagttt ggttttctgg gctgcttgaa tttgggctac ttaaaattaa
    25201 gtagatgtcc aagaccgtag ggatcgtaag atttaatcgt aactgcccta cgcagacggt
    25261 agtcctgaaa cacattgcaa gattgcttgt aagatgtctt tttaggttac cgcgctggtg
    25321 ggatatcgtt ttggtatcct gtttataaac agtgggaggt agaccttgag tctcaatatt
    25381 gaaaccaaga aagtggcggt cgaggaaatt agcgcggcaa ttgctaatgc tcaaaccctc
    25441 gtagtcgctg aatatcgcgg tatcagtgtt tccagtatga ctgagcttcg tgcgaatgca
    25501 cgtaaagaag gcgtttattt gcgcgttctg aaaaatactt tggctcgtcg tgcagtgcaa
    25561 ggtacttcat ttgcagaatt ggccgatcaa atggttggtc cgttggttta cgctgcttct
    25621 gaagatgctg ttgctgctgc taaagtgttg caccaattcg cgaaaaaaga tgacaaaatt
    25681 gtcgttaaag ccggttctta caatggcgaa gtaatgaatg ctgctcaggt tgctgagttg
    25741 gcttctattc cgagccgcga agagctgttg tccaaactgt tgttcgttat gcaagctcct
    25801 gtatcgggct ttgcgcgcgg tttggctgct ttggcagaga aaaaagccgg cgaagaagcc
    25861 gcttaatcga ttttgtttct gttaatcaat tattttttaa tacaatattt ggagtaaaat
    25921 agcatgtcta ttactaaaga agacattttg gaagcagttg gttctttgac cgtaatggaa
    25981 ttgaacgact tggttaaagc ttttgaagaa aaattcggtg tttctgctgc tgctgttgca
    26041 gttgcaggtc ctgctggtgc cggtgctgcc gatgctgaag aaaaaaccga atttgatgtc
    26101 gttttggctt ctgccggcga tcaaaaagtc ggcgtgatta aagttgtccg tgcaattacc
    26161 ggtttgggtc tgaaagaagc taaagacatc gttgacggtg cacctaaaac cattaaagag
    26221 ggtgtttcta aagctgaagc cgaagacatc caaaaacaac tggaagaagc cggcgctaaa
    26281 gtcgaaatca aataatttga tgcttcttat gaaggctggc agttttctgc cagccttatt
    26341 ttgcttctta aaataaacat caagtattgt ttacatttat ttgcatagtt tttatcaaat
    26401 cattgcaaat aaatgtaaac atcagattga tgcgtgccgt tgctttcaaa cggcctatta
    26461 ttgaaaatta cttttcggag tgtatatgag ctattcgttt accgagaaaa aacgtatccg
    26521 taagagtttt gcaaagcggg aaaatgtttt ggaagttcct ttcttgctag caacccaaat
    26581 tgattcttat gcgaagtttt tgcagctgga aaatgctttt gacaaacgta ccgatgacgg
    26641 tctgcaggca gcatttaatt ctattttccc gattgtgagc cataacggtt atgcgcgatt
    26701 ggagtttgtg cattacacat tgggcgagcc tttgttcgat attcccgaat gtcagttgcg
    26761 cggaatcact tatgcagccc ccttgcgcgc gcgtatccgt ttggtgattt tggataagga
    26821 agcatctaaa ccgacggtaa aagaagttcg tgaaaacgaa gtgtatatgg gcgaaattcc
    26881 gttgatgacc ccgagcggtt cttttgtgat taacggcaca gagcgtgtga ttgtctccca
    26941 gttgcaccgt tcgcccggcg tattcttcga gcatgacaaa ggtaagacgc actcttccgg
    27001 caaattgtta ttctccgccc gcatcattcc ctaccgtggt tcatggttgg attttgaatt
    27061 tgatccgaaa gatttgctgt atttccgtat cgaccgccgc cgtaaaatgc cggtaacgat
    27121 tttgttgaag gctttgggct acaacaatga gcaaatcttg gatattttct acgacaaaga
    27181 aacgttctat ttgtcttcaa acggtgttca aaccgatttg gtcgcaggcc gtctgaaagg
    27241 cgaaactgcc aaggtcgata tcttggataa agaaggcaat gtattggttg ccaaaggtaa
    27301 gcgtattact gcgaaaaata tccgtgatat taccaatgca ggcctgaccc gtttggatgt
    27361 agaaccggaa agcctgctgg gcaaagcatt ggctgccgat ctgattgatt cggaaaccgg
    27421 cgaggtattg gcttctgcca atgatgaaat tacagaagag ttgttggcca aatttgatat
    27481 caacggcgta aaagaaatta cgacccttta catcaatgag ttggatcagg gtgcttatat
    27541 ctccaatacc ttgcgtacgg atgagactgc cggccggcag gcggctcgtg ttgcgattta
    27601 ccgtatgatg cgtccgggcg aaccgcccac tgaagaggcg gtcgagcaat tgtttaaccg
    27661 cttgttcttc agtgaagaca gctacgattt gtcccgcgta ggccgtatga aatttaatac
    27721 gcgcacatac gaacaaaaac tgtccgaagc ccaacaaaac tcttggtacg gccgcctgct
    27781 gaacgaaacg tttgccggtg ctgccgacaa aggcggttat gtcctgagcg tcgaagatat
    27841 tgtcgcctcg attgcgactt tggtcgagtt gcgtaacggc catggcgaag tggacgatat
    27901 cgatcacttg ggcaaccgcc gagtacgttc ggtaggcgag ctgactgaaa accaattccg
    27961 cagcggtttg gcccgtgtgg aacgtgctgt aaaagaacgt ttgaatcagg cggaatcaga
    28021 aaacttgatg ccgcacgatt tgattaatgc aaaacctgtt tctgccgcta ttaaagaatt
    28081 cttcggctcc agccaattga gtcagtttat ggatcagacc aaccccttgt ctgaagtaac
    28141 ccataaacgc cgcgtatctg cattgggtcc gggcggtttg acccgcgaac gtgccggctt
    28201 cgaggtacgg gacgtgcatc cgacccacta cggccgcgta tgtccgattg aaacgcctga
    28261 aggtccgaac atcggtttga tcaactcatt gtccgtttat gcgcgcacca atgattacgg
    28321 tttcttggaa acgccttacc gccgcgttat cgacggcaaa gtaaccgagg aaatcgatta
    28381 cttgtctgcc atcgaagaag gccgctatgt gattgcacag gcgaatgccg atttggattc
    28441 agatggcaat ctgattggcg atttggttac ctgtcgtgaa aaaggcgaaa ccattatggc
    28501 aacgcccgac cgcgtccaat atatggacgt ggcaaccggc caggtggtat ccgttgcggc
    28561 atccctgatt ccgttcttgg aacatgatga cgcgaaccgc gcattgatgg gtgccaacat
    28621 gcaacgtcag gcagtgcctt gcttgcgtcc tgaaaaaccg atggtcggta ccggtatcga
    28681 gcgttccgtt gccgttgact ctgctactgc aatcgttgcc cgccgaggcg gcgtggtcga
    28741 gtatgtcgat gccaaccgtg ttgtgattcg tgtccatgac gacgaagcga ctgccggcga
    28801 agtgggtgtc gatatttaca acttggttaa atttacccgt tccaaccagt ctaccaatat
    28861 caatcagcgt cctgccgtca aagccggcga tgttttgcaa cgcggcgatt tggtggccga
    28921 tggcgcgtcc accgatttgg gcgaattggc tttgggtcaa aatatgacca tcgccttcat
    28981 gccgtggaac ggttacaact acgaagactc gattctgatt tccgaaaaag tggctgcgga
    29041 tgatcgttac acttcgattc acattgagga attgaatgtc gttgcccgcg ataccaagct
    29101 gggtgcggaa gacattaccc gcgatattcc gaacttgtcc gagcgtatgc aaaaccgctt
    29161 ggacgaatcc ggtatcgttt acatcggtgc agaagtagaa gccggcgatg tgttggtagg
    29221 caaagtaacg cctaaaggcg aaacccaact gacgccggaa gaaaaactgt tgcgcgccat
    29281 cttcggcgaa aaagcgtctg acgtaaaaga tacttcactg cgtatgccta ccggtatgag
    29341 cggtacggtt atcgacgttc aagtcttcac tcgtgaaggt attcaacgcg acaaacgcgc
    29401 ccaatccatt atcgattccg agttgaaacg ctaccgtttg gatttgaacg accaattgcg
    29461 tattttcgac aacgacgcat tcgaccgtat cgaacgtatg attgtgggtc aaaaagccaa
    29521 tggtggtccg atgaagctgg ctaaaggcag cgaaattact accgaatatt tggcgggtct
    29581 gccgagcagg cacgattggt tcgatatccg tctgaccgat gaagatttgg ccaagcagtt
    29641 ggaactgatt aaagtgagcc tgcaacaaaa acgcgaagaa gcggacgagt tatacgaaat
    29701 caagaagaaa aaactgaccc aaggcgacga attgcaaccc ggcgtacaaa aaatggtgaa
    29761 agtttttatc gccatcaaac gccgtctgca agccggcgac aaaatggcgg gccgccacgg
    29821 taacaaaggc gtggtatcgc gcattctgcc agtggaagac atgccttaca tggcggacgg
    29881 ccgtccggta gacatcgtac tgaacccatt gggcgtacct tcccgtatga acatcggtca
    29941 gattttggaa gttcacttgg gttgggcagc aaaaggtatc ggcgagcgta tcgaccgtat
    30001 gctgaaagag caacgcaaag caggcgagtt gcgcgagttc ttgaacaaac tctacaacgg
    30061 cagcggcaag aaagaagatt tggatgccct gactgatgaa gaaatcatcg aactggcctc
    30121 caacctgcgt aaaggtgcat ctttcgcctc tcctgtattc gacggtgcga aagagtctga
    30181 aatccgcgaa atgttgaact tggcttatcc gagcgacgat cctgaggttg aaaaactggg
    30241 cttcaacgac agtaaaaccc aaatcacgct gtatgacggc cgttcaggcg aagcatttga
    30301 ccgcaaggtt acagtaggtg tgatgcacta tctgaaactg caccacttgg ttgacgaaaa
    30361 aatgcacgcg cgttctaccg gtccgtacag tctggttacc cagcagcctt tgggcggtaa
    30421 agcccagttc ggcggccaac gtttcggcga gatggaggtt tgggcattgg aagcatacgg
    30481 cgcggcatac acgctgcaag agatgctgac tgtgaagtct gacgacgtga acggccgtac
    30541 caaaatgtac gaaaacatcg tcaaaggcga acacaaaatc gatgccggta tgcccgagtc
    30601 cttcaacgta ttggtcaaag agattcgctc actgggcttg gatatcgatt tggaacgtta
    30661 ctaaacaaaa gttttcagac ggcctttcag ggtcgtctga aaaagtggtt tcagaataag
    30721 aatgaagcaa tcggcattta ggccgtctga aatcaaaagt accgtttccc aatatcgaaa
    30781 atccgccatg cggtaaaaat acttccttca aggagcaaaa atgaatttgt tgaacttatt
    30841 taatccgttg caaactgccg gcatggaaga agagtttgat gccattaaaa tcggtattgc
    30901 ctctcccgaa accatccgct catggtctta tggcgaagtc aaaaaacctg aaaccatcaa
    30961 ctaccgtacg ttcaaacctg agcgtgacgg tttgttctgt gccaaaatct ttggcccggt
    31021 caaagactac gaatgcttgt gcggaaaata caaacgcttg aaatttaaag gtgtaacttg
    31081 tgaaaaatgc ggcgtggaag taaccctgtc caaagtgcgc cgcgaacgca tgggccatat
    31141 cgaattggct gcgcccgtcg cacatatttg gttcttaaaa tccctgcctt cccgcttggg
    31201 tatggtgtta gacatgactt tgcgcgatat cgaacgcgta ttgtactttg aagcatttgt
    31261 ggtaaccgat cccggtatga ctccgctgca acgccgccaa ttgctgactg aagacgatta
    31321 ctacaacaag ctggacgaat acggcgacga tttcgatgcc aaaatgggtg cggaaggtat
    31381 ccgcgaattg ctgcgtaccc tgaatgtagc gggcgaaatc gaaatcctgc gccaagagtt
    31441 ggaatcgacc ggttccgaca ccaaaatcaa aaaaatcgcc aaacgcttga aagtattgga
    31501 agctttccat cgttccggta tgaaactgga atggatgatt atggatgtgc tgccggtatt
    31561 gccgcctgat ttgcgtccgt tggttccatt ggatggtggt cgttttgcca cttccgattt
    31621 gaacgatttg taccgccgcg ttattaaccg taacaaccgt ctgaaacgtc tgttggaact
    31681 gcatgctcct gacatcatcg tccgcaacga aaaacgtatg ttgcaagaag cagttgactc
    31741 gctgttggat aacggccgtc gcggtaaagc catgaccggc gccaacaaac gcccgctgaa
    31801 atcattggca gacatgatta aaggtaaggg tggccgcttc cgtcaaaacc tgctgggcaa
    31861 acgtgtggac tactccggcc gttccgtgat taccgtaggc ccgtacctgc gtctgcacca
    31921 atgcggtttg ccgaaaaaaa tggctttgga actgttcaaa ccgttcattt tccacaaatt
    31981 ggaaaaacaa ggtttggcct ctaccgttaa agcagcgaaa aaattggtag agcaagaagt
    32041 accggaagta tgggacatct tggaagaagt catccgcgaa catccgatta tgctgaaccg
    32101 tgcgccgacc ctgcaccgtt tgggtattca agcgttcgaa cctatcttga ttgaaggtaa
    32161 agcgattcag ttgcacccat tggtgtgtgc tgcgttcaac gccgactttg acggtgacca
    32221 aatggctgta cacgttccat tgagcttgga agcacaaatg gaagcgcgca ccctgatgct
    32281 ggcttcaaac aacgtattgt ctccagccaa cggcgaaccg attatcgtac cttcccaaga
    32341 catcgtattg ggtttgtact acatgacccg cgaccgtatc aatgccaaag gcgaaggcag
    32401 cctgtttgcc gatgtgaaag aagtgcatcg tgcataccat accaaacagg tcgagctggg
    32461 tacgaaaatt accgtacgtc tgcgcgaatg ggtgaaaaac gaagcaggtg agtttgaacc
    32521 tgtcgttaac cgttacgaaa caaccgtcgg ccgtgcattg ttgagcgaaa tcctgccgaa
    32581 aggcctgccg tttgaatatg tcaacaaagc gttgaagaaa aaagaaattt ctaaactgat
    32641 taacgcatcg ttccgcctgt gcggcttgcg cgatacggtt atctttgctg accacctgat
    32701 gtacaccggt ttcggatttg cggcaaaagg cggtatttcc attgccgttg acgatatgga
    32761 aattccaaaa gaaaaagcgg ccttgctggc tgaagccaat gccgaggtta aagaaatcga
    32821 agaccaatac cgtcaaggtt tggttactaa cggcgaacgc tacaacaagg tggtcgatat
    32881 ttggggtcgt gccggcgata agattgctaa agcgatgatg gacaacttgt ccaaacaaaa
    32941 agttatcgac cgtgacggca acgaagtcga tcaagagtca ttcaactcca tttatatgat
    33001 ggcggactcc ggtgcccgtg gttctgcagc tcagattaaa cagttgtccg gtatgcgtgg
    33061 cttgatggct aaacctgacg gctcgattat tgaaacgccg attacctcaa acttccgtga
    33121 aggtctgacc gtattgcaat actttattgc gacccacggt gcgcgtaagg gtttggcgga
    33181 taccgcattg aaaaccgcga actccggtta cctgacccgt cgtctggtag acgtaactca
    33241 agatttggtc gttgttgaag acgattgcgg tacttcagac ggctttgtca tgaaggcagt
    33301 ggtacaaggc ggtgatgtga ttgaagcatt gcgcgatcgt attttgggcc gtgttactgc
    33361 gtctgacgtt gtcgatccgt caagcggcga gaccttggtt gaagccggta cgttgttgac
    33421 tgaaaaactg gtggatatga ttgaccaatc cggtgtcgat gaagtcaaag tccgtacgcc
    33481 gattacttgt aaaacccgcc acggtttgtg cgcgcactgt tatggtcgcg acttggcgcg
    33541 cggtaagctg gttaacgccg gtgaagcagt tggtgtgatt gctgcacaat ccattggtga
    33601 accgggtacc cagttgacca tgcgtacgtt ccacattggt ggtgcggcat cccgtgcggc
    33661 agcagccagc caagttgaag ccaaatccaa cggtacggca cgattcagca gccaaatgcg
    33721 ttatgttgcc aacaacaaag gcgaattggt tgtcatcggc cgctcttgcg aagtcgtgat
    33781 tcatgatgac atcggccgtg aacgcgaacg ccacaaagta ccttacggtg ccatcctgct
    33841 ggtacaagac ggtatggcca ttaaagccgg tcaaaccttg gcaacttggg atccgcatac
    33901 ccgtccgatg attaccgaac acgcaggtat ggtgaaattt gagaacgtgg aagagggtgt
    33961 taccgttgcg aaacaaactg acgatgtaac cggtttgtcc actctggtag tgattgacgg
    34021 taaacgtcgt tcctctagtg cttctaaact gctgcgtccg actgtaaaat tgttggatga
    34081 aaacggcgtg gaaatctgta ttcccggtac gtccactccg gtttcgatgg cattccccgt
    34141 tggtgcggtg attaccgtac gcgaaggtca ggaaatcggt aaaggcgacg tattggcgcg
    34201 tatcccgcaa gcctcttcca aaacccgcga cattaccggt ggtctgccgc gcgttgccga
    34261 gctgtttgaa gcacgcgtgc cgaaagatgc cggtatgctg gcggaaatta ccggtaccgt
    34321 ttccttcggt aaggagacca aaggcaaaca acgtctgatt attaccgacg tggacggcgt
    34381 agcatacgag actttgattt ccaaagagaa acaaattctg gtacacgacg gtcaagtggt
    34441 aaaccgaggc gaaaccattg tggacggcgc ggtcgatccg cacgatattc tgcgtttgca
    34501 aggtatcgaa gcattggcac gctacattgc ccaagaggtg caagaggttt accgtctgca
    34561 aggtgtgaag atttctgata aacacatcga agtcatcatc cgtcaaatgt tgcgccgtgt
    34621 gaacattgcg gatgccggcg aaaccgggtt cattaccgga gagcaggtcg aacgcggcga
    34681 tgtgatggcg gccaatgaaa aagctttgga agaaggcaaa gaaccggcgc gttacgaaaa
    34741 cgtattgctg ggtattacca aagcttccct gtctaccgac agcttcattt ctgctgcatc
    34801 gttccaagaa acgacccgtg ttctgaccga agccgcgatt atgggcaaac aagacgaact
    34861 gcgcggttta aaagagaacg tcatcgtcgg ccgcctgatt cctgccggta ccggtttgac
    34921 ttatcaccgc agccgtcatc agcaatggca aggggtagag caggagactg ctgaaaccca
    34981 agtaacggat gaataatctt cggcgcgttt attcaataca aaaccgcaag ctttaagctt
    35041 gcggtttttc tttgtccgat taaggcaaaa acaagcgttt tcgtcatttt gaggcgtgtg
    35101 gattattcct taggtatttt cgggccggag accaacgagg tggcgggtgt cgtcggtacg
    35161 tccggagacc aaaataactt tgccaaggat gttggtttcg gcggtcaaaa aagtagcgtc
    35221 ttaatgtttt ccatttaaac aaatgtcgtc tgaaacttca gacggcattt cctttaagaa
    35281 ataaatatga aacccagaaa tctctttttt gcaggctgcc tgctgacttc ggcgacgttt
    35341 gccgaggata tcggcgtacc tgtcgaactg attaacgtcg gtaatcggat tgcgatgccg
    35401 tctgaagggg aaagcctcgc cctcctgccg tttgccgagg atgtaccgcc ggttcgcgat
    35461 gcaatgccgt ctgaagttcc taaaagcgcg gcaggcggcg atgttcgggg tgaccggata
    35521 ggaatgccga ttaacatcgg atgagcgcgg ctttatggca taaaaaactg tcgtggaaag
    35581 gatttacacc ccaaataaat ttccgttaca acaagatcaa cagcaatatg cccgcctttt
    35641 attcgcgcag cggcaaggaa cggtttgtca gtatagaaaa aacgtattga cagtattttc
    35701 ttcagtcgtc cgactgattg tgagggatgt cggtaaatat ttatcggcaa acaagaaaat
    35761 catctttctt cttgtcgtta tgcttgactg tatacttgca ataaaaatat aattccactc
    35821 ttgccgacat ggtgtcggca agtatttaac tcaacaggac gagaaaatat gccaactatc
    35881 aaccaattgg tacgcaaagg ccgtcaaaag cccgtgtacg taaacaaagt gcccgcactg
    35941 gaagcctgcc cgcaaaaacg cggcgtgtgc acccgtgtat acacgactac ccctaaaaaa
    36001 cctaactctg cattgcgtaa agtatgtaaa gtccgcctga ccaacggttt tgaagtcatt
    36061 tcatacatcg gcggcgaagg ccacaacctg caagagcaca gtgtcgtatt gattcgcggc
    36121 ggtcgtgtaa aagacttgcc aggtgtgcgt taccacactg tacgcggttc tttggatact
    36181 gcaggcgtta aagaccgtaa acaagcccgt tccaaatacg gtgctaagcg tcctaaataa
    36241 ttactgggac ttaaataggc acgtcggccg cctaagctga acaacggccg agtaagtgaa
    36301 tactcaattg ggtattcatg ggaatagacc cgactgaata gattaaagga aattaaaatg
    36361 ccaagacgta gagaagtccc caagcgcgac gtactgccag atcctaaatt cggcagcgtc
    36421 gagttgacca aattcatgaa cgtattgatg attgacggta aaaaatccgt tgccgagcgt
    36481 atcgtttacg gtgcgttgga acagattgag aaaaaaaccg gcaaagtagc aatcgaagta
    36541 tttaacgaag ccattgcaaa cgccaaacct atcgtggaag tgaaaagccg ccgtgtaggt
    36601 ggtgcaaact accaagttcc tgttgaagtt cgtccttcac gccgtttggc tttggcaatg
    36661 cgctgggttc gcgatgcggc ccgcaaacgt ggtgagaaat ccatggacct gcgtttggca
    36721 ggcgaattga ttgatgcgtc cgaaggccgt ggcggtgcgt tgaaaaaacg tgaagaagta
    36781 caccgtatgg ctgaagccaa caaagcattc tctcacttcc gtttctaatt ttgaaaggct
    36841 aataaaatgg ctcgtaagac cccgatcagc ctgtaccgta acatcggtat ttccgcccat
    36901 atcgatgcgg gtaaaaccac gacgacagaa cgtattttgt tctataccgg tttgacccac
    36961 aagctgggcg aagtgcatga cggtgcggct actaccgact acatggaaca agagcaagag
    37021 cgcggtatta ccattacctc cgctgccgtt acttcctact ggtccggtat ggcgaaacaa
    37081 ttccccgagc accgcttcaa catcatcgac accccgggac acgttgactt taccgtagag
    37141 gtagagcgtt ctatgcgtgt attggacggc gcggtaatgg tttactgcgc ggtgggcggt
    37201 gttcaacccc aatctgaaac cgtatggcgg caagccaaca aataccaagt gccgcgcttg
    37261 gcgtttgtca ataaaatgga ccgtcagggt gccaacttct tccgcgttgt cgagcaaatg
    37321 aaaacccgtt tgcgcgcaaa ccctgtacct atcgtcattc cggttggtgc ggaagacaac
    37381 ttcagcggtg tggttgattt gttgaaaatg aaatccatca tttggaatga agccgataaa
    37441 ggtacaacct ttacctatgg cgatattcct gccgaattgg tcgaaactgc cgaagaatgg
    37501 cgtcaaaata tgattgaagc cgcagccgaa gccagcgaag aactgatgga caaatactta
    37561 ggcggcgacg agctgaccga agaagaaatc gtaggcgcgt tgcgtcaacg tactttggca
    37621 ggcgaaattc agcctatgct gtgtggttct gcatttaaaa acaaaggtgt tcaacgtatg
    37681 ttggacgcag ttgtagaatt gctgccagct cctaccgata ttcctccggt tcaaggtgtc
    37741 aacccgaata ccgaggaagc cgacagccgt caagccagcg atgaagagaa attctctgca
    37801 ttggcgttca aaatgttgaa cgacaaatac gtcggtcagc tgacctttat ccgcgtttac
    37861 tcaggcgtag taaaatccgg cgataccgta ttgaactccg taaaaggcac tcgcgaacgt
    37921 atcggtcgtt tggtacaaat gactgccgca gaccgtactg aaatcgaaga agtacgcgcc
    37981 ggcgacatcg cagccgctat tggtctgaaa gacgttacta ccggtgaaac cctgtgtgcg
    38041 gaaagcgcac cgattatctt ggaacgtatg gaattccccg agccggtaat ccatattgcc
    38101 gttgagccga aaaccaaagc cgaccaagag aaaatgggta tcgccctgaa ccgcttggct
    38161 aaagaagacc cttctttccg tgtccgtaca gacgaagaat ccggtcaaac cattatttcc
    38221 ggtatgggtg agctgcactt ggaaattatt gttgaccgta tgaaacgcga attcggtgtg
    38281 gaagcaaata tcggtgcacc tcaagtggct taccgtgaaa ctatccgcaa agccgttaaa
    38341 gctgaataca aacatgcaaa acaatccggt ggtaaaggtc aatacggtca cgttgtgatt
    38401 gaaatggaac ctatggagcc ggggggtgaa ggttacgagt ttatcgatga aattaaaggt
    38461 ggtgtgattc ctcgcgaatt cattccgtct gtcgataaag gtatccgcga tacgttgtct
    38521 aacggtatcg ttgccggcta tcctgtagtt gacgtacgta tccgtctggt attcggttct
    38581 taccatgatg tcgactcttc ccaattggca tttgaattgg ctgcttctca agcgtttaaa
    38641 gaaggtatgc gtcaagcatc tcccgccctg cttgagccga ttatggcagt tgaagtggaa
    38701 accccggaag aatacatggg cgacgtaatg ggcgacttga accgccgtcg cggtgttgta
    38761 ttgggtatgg atgatgacgg tatcggcggt aaaaaagtcc gtgccgaagt acctctggca
    38821 gaaatgttcg gttattcgac cgacctgcgt tctgcaaccc aaggccgcgc tacttactct
    38881 atggagttca agaaatattc tgaagctcct gcccacatag ctgctgctgt aactgaagcc
    38941 cgtaaaggct aatcaggcaa ataggtcgtc tgaaaggctg aaatgatttt tcagacggca
    39001 ttgttcttta atcgatcttt aatgtaaagg aattagctca tggctaagga aaaattcgaa
    39061 cgtagcaaac cgcacgtaaa cgttggcacc atcggtcacg ttgaccatgg taaaaccacc
    39121 ctgactgccg ctttgactac tattttggct aaaaaattcg gtggtgctgc aaaagcttac
    39181 gaccaaatcg acaacgcacc cgaagaaaaa gcacgcggta ttaccattaa cacctcgcac
    39241 gtggaatacg aaaccgaaac ccgccactac gcacacgtag actgcccggg gcacgccgac
    39301 tacgttaaaa acatgattac cggcgccgca caaatggacg gtgcaatcct ggtatgttcc
    39361 gcagccgacg gccctatgcc gcaaacccgc gaacacatcc tgctggcccg ccaagtaggc
    39421 gtaccttaca tcatcgtgtt catgaacaaa tgcgacatgg tcgacgatgc cgagctgttg
    39481 gaactggttg aaatggaaat ccgcgacctg ctgtccagct acgacttccc cggcgatgac
    39541 tgcccgattg tacaaggttc cgcactgaaa gccttggaag gcgatgccgc ttacgaagaa
    39601 aaaatcttcg aactggctgc cgcattggac agctacatcc cgactcccga gcgtgccgtg
    39661 gacaaaccgt tcctgctgcc tatcgaagac gtgttctcca tttccggccg cggtacagta
    39721 gtaaccggcc gtgtagagcg cggtatcatc cacgttggtg acgagattga aatcgtcggt
    39781 ctgaaagaaa cccaaaaaac cacctgtacc ggtgttgaaa tgttccgcaa actgctggac
    39841 gaaggtcagg cgggcgacaa cgtaggcgta ttgctgcgcg gtaccaaacg tgaagacgtg
    39901 gaacgcggtc aggtattggc taaaccgggt actatcactc ctcacaccaa attcaaagca
    39961 gaagtatacg tactgagcaa agaagagggt ggtcgtcaca ctccgttctt cgccaactac
    40021 cgtccgcaat tctacttccg taccaccgac gtaaccggcg cggttacttt ggaagaaggt
    40081 gtggaaatgg taatgccggg tgaaaacgta accatcaccg tagaactgat tgcgcctatc
    40141 gctatggaag aaggcctgcg ctttgcgatt cgcgaaggcg gccgtaccgt gggtgccggc
    40201 gtggtttctt ctgttatcgc ttaattgaag gatattgata aatggcaaac caaaaaatcc
    40261 gtatccgcct gaaagcttat gattacgccc tgattgaccg ttctgcacaa gaaatcgttg
    40321 aaactgcaaa acgtaccggt gcagttgtaa aaggcccgat tcctttgccg accaaaatcg
    40381 agcgtttcaa cattttgcgt tctccgcacg tgaacaaaac ttcccgtgag caattggaaa
    40441 tccgcaccca cttgcgcctg atggacatcg tggattggac cgataaaact accgatgcgc
    40501 tgatgaagct ggatttgccg gccggtgttg atgtagaaat caaagtccaa taattcggac
    40561 tattgaaaat ccccaagcaa tcaatgcttg gggatttttt atgttatgcc gagacctttg
    40621 caaaattccc caaaatcccc taaattccca ccaagacatt taggggattt ctcatgagca
    40681 ccttcttcca gcaaacctcc caagccatga ttgccaaaca catcgaccgg ttcccactat
    40741 tgaagttgga ccgggtaatt gattggcagc cgatcgaaca gtacctgaat cgtcaaa
//
LOCUS       DAWFJR010000008        39680 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWFJR010000008 DAWFJR010000000
VERSION     DAWFJR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39680
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.008"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            180..530
                     /locus_tag="WGR04_000355"
                     /pseudo
     CDS             180..530
                     /locus_tag="WGR04_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
     gene            640..1056
                     /locus_tag="WGR04_000356"
     CDS             640..1056
                     /locus_tag="WGR04_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ0793985.1"
                     /translation="MIDVKIDNIFVVLNQIEQLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            1287..2348
                     /locus_tag="WGR04_000357"
     CDS             1287..2348
                     /locus_tag="WGR04_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793986.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFGLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAEGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
     gene            2398..2736
                     /locus_tag="WGR04_000358"
                     /pseudo
     CDS             2398..2736
                     /locus_tag="WGR04_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2810..3421
                     /locus_tag="WGR04_000359"
     CDS             2810..3421
                     /locus_tag="WGR04_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ0793987.1"
                     /translation="MAAPAGVATECRLTVQGGTDRESDASLLARLLEIIRRPPAGGNR
                     YDYKNWALSVDGVTSAYVYPLRRGLGTVDIAITSADGVSSEETVRRVQAYIDEMRPVT
                     AKNALVLKPTVTAVPVTVQVKLDGIDLDEAKRRIRTALKEYFDTLIPGDGLTVSQIEA
                     AISNVDGVIDRRLTAPTANRAADTVNRIEWFKAGAINVTEMPS"
     gene            3418..3978
                     /locus_tag="WGR04_000360"
     CDS             3418..3978
                     /locus_tag="WGR04_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ0793988.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            3989..5962
                     /locus_tag="WGR04_000361"
     CDS             3989..5962
                     /locus_tag="WGR04_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ0793989.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVITGDQTITGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDNTLRLHGANNRISYNNHDIFHKANKPRFAEDIESKPNTLSGY
                     GIRNFKVETFRGDLNTLKTDGIYSLSTAVGSSNLPIENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVRNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWTNHTDTA
                     AVGYEDRNERQRSALVSTWTDENLNDNGFLTPRPDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQGLQDKADRDHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGARRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(6591..6893)
                     /locus_tag="WGR04_000362"
     CDS             complement(6591..6893)
                     /locus_tag="WGR04_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ0793990.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
     gene            complement(6865..7152)
                     /locus_tag="WGR04_000363"
     CDS             complement(6865..7152)
                     /locus_tag="WGR04_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ0793991.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVRFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            7232..7837
                     /locus_tag="WGR04_000364"
     CDS             7232..7837
                     /locus_tag="WGR04_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793992.1"
                     /translation="MTIYFKNGFYDDTLGSIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVVEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            7816..8112
                     /locus_tag="WGR04_000365"
     CDS             7816..8112
                     /locus_tag="WGR04_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ0793993.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGVFFDRQHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            8109..8318
                     /locus_tag="WGR04_000366"
     CDS             8109..8318
                     /locus_tag="WGR04_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0793994.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPAYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(8428..9571)
                     /locus_tag="WGR04_000367"
                     /pseudo
     CDS             complement(8428..9571)
                     /locus_tag="WGR04_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(10013..10660)
                     /locus_tag="WGR04_000368"
     CDS             complement(10013..10660)
                     /locus_tag="WGR04_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ0793995.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(10657..11025)
                     /locus_tag="WGR04_000369"
     CDS             complement(10657..11025)
                     /locus_tag="WGR04_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ0793996.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(11035..11706)
                     /locus_tag="WGR04_000370"
     CDS             complement(11035..11706)
                     /locus_tag="WGR04_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ0793997.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVEKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(11871..12590)
                     /locus_tag="WGR04_000371"
     CDS             complement(11871..12590)
                     /locus_tag="WGR04_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0793998.1"
                     /translation="MATLSDKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGYPEPFAICFDLISAEEKEFEHFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASTESKGRSGEIVYL"
     gene            complement(12673..13986)
                     /locus_tag="WGR04_000372"
     CDS             complement(12673..13986)
                     /locus_tag="WGR04_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEZ0793999.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFASAEACLQSLSDAV"
     gene            complement(14065..14937)
                     /gene="hpnD"
                     /locus_tag="WGR04_000373"
     CDS             complement(14065..14937)
                     /gene="hpnD"
                     /locus_tag="WGR04_000373"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ0794000.1"
                     /translation="MKGLDYCHQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVRARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            15108..16970
                     /gene="hscA"
                     /locus_tag="WGR04_000374"
     CDS             15108..16970
                     /gene="hscA"
                     /locus_tag="WGR04_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ0794001.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTLQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVKQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVA
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVHAQIEVKPSYGL
                     DDDTITQMLKDSMSNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEELQQI
                     RQGIADLQGRLKDGKAEDIRAAAAKLGSITDNFAAKRMNRNIQRALTGQSVDNI"
     gene            17233..17856
                     /locus_tag="WGR04_000375"
     CDS             17233..17856
                     /locus_tag="WGR04_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0794002.1"
                     /translation="MFVNSLYKIGISEVSNFEGRMRHLENNGYANVAGLERILAVKTD
                     NYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKNETAESEFEKSV
                     HERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSKIRLFKEAYFGK
                     SGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPQ"
     gene            17893..18654
                     /locus_tag="WGR04_000376"
     CDS             17893..18654
                     /locus_tag="WGR04_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ0794003.1"
                     /translation="MMGDSVIYYVEQADKPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESVCV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLAGWQNVADLLEPRH"
     gene            18709..19050
                     /gene="fdx"
                     /locus_tag="WGR04_000377"
     CDS             18709..19050
                     /gene="fdx"
                     /locus_tag="WGR04_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ0794004.1"
                     /translation="MPKITVLPHTTLCPEGAVIEDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(19337..19924)
                     /locus_tag="WGR04_000378"
     CDS             complement(19337..19924)
                     /locus_tag="WGR04_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ0794005.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRGLIALDKAAAGCDAAMLRAQRWLIYTQIAERLAGSGGGFT
                     VTVESVSAACLELEGRYLELVRRAAVSFGFVQVQEYPAGGQEETQAVPDAASDHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            20137..20334
                     /gene="iscX"
                     /locus_tag="WGR04_000379"
     CDS             20137..20334
                     /gene="iscX"
                     /locus_tag="WGR04_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ0794006.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(20402..20803)
                     /locus_tag="WGR04_000380"
     CDS             complement(20402..20803)
                     /locus_tag="WGR04_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0794007.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTAALREERKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            21014..21973
                     /locus_tag="WGR04_000381"
     CDS             21014..21973
                     /locus_tag="WGR04_000381"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ0794008.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            22041..23351
                     /gene="tilS"
                     /locus_tag="WGR04_000382"
     CDS             22041..23351
                     /gene="tilS"
                     /locus_tag="WGR04_000382"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ0794009.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKICVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHLLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNVQGFELHHYAGRLFVFRPEKTDKLRFLKDRQISGNLR
                     EILAGQGFVLKRHPFGLPGHLLEQDGILRTVAASDRLAVGGIHKDVKKILQEKRVLPA
                     LRPFWPVVADKDNRPLALANYGTDFQISVSDGILPVHPDFPVLF"
     gene            23402..24187
                     /locus_tag="WGR04_000383"
     CDS             23402..24187
                     /locus_tag="WGR04_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0794010.1"
                     /translation="MKHISSANNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGRPVGVYIPEAKMPSEEVRKLTAVLPEDGVFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGIGAVVLGKGCADAWSPKVLRAGM
                     GAHFLSEIYPQADLEIWLARYEDRVFATALREEKQAVLYNEDLRKPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            24197..24394
                     /locus_tag="WGR04_000384"
     CDS             24197..24394
                     /locus_tag="WGR04_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794011.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLAWVWWKWF"
     gene            24656..25009
                     /locus_tag="WGR04_000385"
     CDS             24656..25009
                     /locus_tag="WGR04_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794012.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRFVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSAHKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            25021..25326
                     /locus_tag="WGR04_000386"
     CDS             25021..25326
                     /locus_tag="WGR04_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794013.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPRENTHKDASNTGAKTH"
     gene            25388..26764
                     /gene="mpl"
                     /locus_tag="WGR04_000387"
     CDS             25388..26764
                     /gene="mpl"
                     /locus_tag="WGR04_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ0794014.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIICNGQQQSLQDTLDKGCWTPVEKFGTEHGWQVGEVNADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHVGVDIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKAALPASLKEADQ
                     VFCYAGGADWDVAEALAPLGGRLHVGKDFDAFVAEIVKNAEAGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            26971..28023
                     /gene="bioB"
                     /locus_tag="WGR04_000388"
     CDS             26971..28023
                     /gene="bioB"
                     /locus_tag="WGR04_000388"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ0794015.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLDDAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFELE
                     GEVAEVEKASGIKVDY"
     gene            complement(28101..28735)
                     /locus_tag="WGR04_000389"
                     /pseudo
     CDS             complement(28101..28735)
                     /locus_tag="WGR04_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            29058..29318
                     /locus_tag="WGR04_000390"
     CDS             29058..29318
                     /locus_tag="WGR04_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ0794016.1"
                     /translation="MTKAVRRKNVQPPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(29381..31240)
                     /gene="ilvD"
                     /locus_tag="WGR04_000391"
     CDS             complement(29381..31240)
                     /gene="ilvD"
                     /locus_tag="WGR04_000391"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ0794017.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHVGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTHAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(31413..33182)
                     /gene="cysI"
                     /locus_tag="WGR04_000392"
     CDS             complement(31413..33182)
                     /gene="cysI"
                     /locus_tag="WGR04_000392"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ0794018.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPVRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEEFARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILSELDDLIGKWAAGRDTDEGFGDFAIRTGI
                     VKPVLDAPVDFWDASKAVPIARA"
     gene            complement(33209..35023)
                     /locus_tag="WGR04_000393"
     CDS             complement(33209..35023)
                     /locus_tag="WGR04_000393"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ0794019.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPTAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVSRAELKD
                     YKAKNIAGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDKRFEELGAKRLLERVDADLDFAAAANAWTDNIAALLKEEAAKNRATP
                     APQATPPAGLQTASEGRYCKADPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFARDFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(35020..36306)
                     /locus_tag="WGR04_000394"
     CDS             complement(35020..36306)
                     /locus_tag="WGR04_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ0794020.1"
                     /translation="MTAQHQAPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLDCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAGRLGLKDVHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ETLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(36345..37268)
                     /gene="cysD"
                     /locus_tag="WGR04_000395"
     CDS             complement(36345..37268)
                     /gene="cysD"
                     /locus_tag="WGR04_000395"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ0794021.1"
                     /translation="MTKTEPNNVQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVTDISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(37306..38040)
                     /locus_tag="WGR04_000396"
     CDS             complement(37306..38040)
                     /locus_tag="WGR04_000396"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ0794022.1"
                     /translation="MKIFKPALWKIPPIENGSETALAEKTETLKQRLHRIAGSHRDAR
                     FASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHAETLNLLDRIERVYPHMQIKRFQP
                     IREDALHYVESKGRFAFYDSVEARRECCRIRKTEPLDRAIAGADAWLTGQRREQSATR
                     TELPFAEYDAGRGIDKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCTRP
                     VKAGEDIRAGRWWWEDKNSKECGLHK"
     gene            complement(38056..39507)
                     /gene="cobA"
                     /locus_tag="WGR04_000397"
     CDS             complement(38056..39507)
                     /gene="cobA"
                     /locus_tag="WGR04_000397"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0794023.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKAKLKSVTERRRFWEKQFNGRFAALVKNQQTAQAEQELAKQLEQNYQGGFVSL
                     VGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGGRTP
                     QEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAAVGA
                     TAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALIAER
                     LQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLHEKL
                     AWFGENAKKESNPAEHAYFALDGLGTGQEQQAA"
BASE COUNT         8925 a        10603 c        11258 g         8894 t
ORIGIN      
        1 gccaacaggc taagtgcgcc cgctgccgcc taaaaagcga ccggatgcct gattatcggg
       61 tatccaggga agattaaggg ggtatttggg taaaatcagg aggtatttgg ggcgaaaaca
      121 gccgaaaacc tgtgtttggg tttcggctgt cgggagggaa aggaattttg caaaggtctc
      181 agcttaaatt tgctgcgggt gtattgagca aggaaatgca agaactggca ggtatgactc
      241 gagcgacggt gtggctgtct gatgatacat tggtcaaaca ggtggacagt cgggaggggc
      301 agaatttcga tgactcctac tatgcttttt tgccggatat gctgcaaaac cctgaacatg
      361 tcatccgcga caatcgtgaa ttgattttca cagctcgcta taaaggctcg gcattgtggg
      421 cagttttaaa atatattaag gaggtggatg agatttatct acagtcgtac cgaatcagta
      481 acgacaaaga gattgccaaa tttatggcga agaagaaagt attgaaatag acgttgggca
      541 aggctcgaat tcacttgcac acgctctcgg acaccctaaa gggcaggctg cggtatcgag
      601 attatcaccg cttttccaac gcctataagg aatcatacca tgattgatgt caaaatagac
      661 aatatctttg tcgtcctaaa ccaaatcgag cagcttggca acgggatcga aaaccgctac
      721 ctgctgatgc gccgactgtc cgaaaccatg cacacggcgg tcaagctcaa tttccgctac
      781 gcaggccgtc cgaaatggtt ggggctaaaa taccgcgacg gcaagccgct ttcggattcg
      841 ggtcgtctga aagacagttt ttccacactg tcagacaacg ataccgccct tgtcggtacg
      901 aatatcgtct atgccgccat ccacaacttc ggcggtatgg cggggcgcaa ccgcaaagtt
      961 cggattccgc aacgggaatt tttgacgctg acggacgacg acaaacaggc tttgatggac
     1021 gatgtgcagg attatttttc gggtctgata ccgtgaattt ataaaaccct caaaaacgcg
     1081 ctttttagcg cgttttttta tgcgggtaat acaaacccct gcccaagata taaaaatcaa
     1141 tcctagacgc ttctaaaaag cccctgaaaa cgattaattg tgtatcgcgc ggacaggttt
     1201 taaaaaaatg gcgggagggt ttgaagcacg cctactcttt gttgtttttt caaataggca
     1261 aaatgaccgt attgagagag gtacacatgt ccaaaaatgc acaaaaaacc ctacttgccg
     1321 tgtgcagttt cgaggtgcag ccaaaagacg ggcgaatcca actgctgcca tatggcgaat
     1381 ttcgcgcagt agacggtcgt ccgactgatg tccctgcgtg gtatctgacc gaagaaaacg
     1441 gtcatgatgt cgcgttgttg gccaacagct cgcgcaatca gttggttgtc gattatgaac
     1501 accagacgct ctacaaagag aaaaacggac aacctgcacc tgccgccggt tggatgcgtt
     1561 ggctggagtt cacgcctaaa ggcatgtttg ccgaagtgga gtggacggac aaggcggctg
     1621 cggcaattgc cgcaaaagag tatcgctaca tctctgctgt gttttcctat gacacaaagg
     1681 gatatgtaag caaaattttt cacgcagcgc tgacaaattt ccccgcgttg gacggtatgg
     1741 acgaggtgct ggcggcagcg tcggcgcaaa ttttaaaacc ggaaacggag caaaacccta
     1801 tgaaagagtt gttacagcaa ctgttcggtc tgcctgatgc gggcgaagaa gaactgaagg
     1861 cggcattgtc cgcgctcgtg gaagccaagc cgaaagacgt ggcattgtct gccgacgtgt
     1921 tcgcgcagct ggcggaaaaa gacagccgca tcgcggcatt gacggcgcaa accgccaagc
     1981 ctgatttgac taaatacgcg cctatctcag tggttcaaga gctgcaaagc aaagtcgccg
     2041 cgctgactgc caagcaggaa gcagacaaag gcaacgaatt gattaccgcc gcgctgactt
     2101 caggcaaatt gctgcctgct cagaaggagt gggcagaagg cgtattgaaa cagccgggcg
     2161 gcttggcatt tttgaccggc tttattgaaa acgcccagcc ggtcgctgca ctggcaggct
     2221 cgcaaacggg cggcaaagca cccgacgaac gcgtcgccgc actgactgcg gaagaggcag
     2281 ccgcagcaaa aatgctgggc atgtccggcg aagaatttgt aaaaatcaaa gaaagcgaag
     2341 gtaagtaatg gacaaatcag cgattttgac cgcaatcacg gcagcattcc gcaaagaatt
     2401 tcaaacgggg gcctgcaaaa ccgcctgaac cgtttaaccg ccaaacaggt gcagccggta
     2461 gcgcaggcag tgcgcctgtt atccacgtca tcgctgttgt cggtggcaac ggcattaatc
     2521 gaggcgcatg gcgaagagat gaccgcgccc gatttgattg aggttaaccg cgccatgcgc
     2581 cgccgtatgc aggccgagat tgccgccttg cgggcggtgc agacggctgc tgccgagtct
     2641 ggtgggctga cggccaacgc cgtgtatacc gaggcttacc aaacggcaga atccctgcgc
     2701 gcggcggcag gccgtctgaa tgcgttggtt gcggcggcac ggcggaaata ccggcaatcg
     2761 ccgacgagcc gggcgcggcc gccaatgtgg cgacggcgag gcgcaactga tggccgcccc
     2821 cgccggtgtg gccaccgaat gccgccttac cgtacaaggc ggcaccgacc gagaaagcga
     2881 tgcctcactg ctggcgcgtc tgttggaaat catccgccga ccgcccgcag gcggcaaccg
     2941 ttacgactat aaaaactggg cgttgagtgt tgacggcgta accagcgcat atgtttatcc
     3001 gctgcgccgc ggcttgggta cggtggatat tgccattacc tccgccgacg gtgtgtcgtc
     3061 ggaagaaact gtgcgccgcg tacaggctta tatcgacgag atgcgcccgg taacggcaaa
     3121 aaatgcgctg gtactcaagc caaccgtaac ggcggtgcct gttaccgtgc aagtcaagct
     3181 cgacggcatc gacttggacg aggccaagcg ccgcatacgg acggccctaa aagaatattt
     3241 cgacaccctg atccccggcg acggcctgac tgtgtcgcaa atcgaggctg ctatcagcaa
     3301 tgtggatggt gtgatcgacc gccgtctgac tgcgccgacg gccaaccgtg ccgccgatac
     3361 ggttaaccgc atcgagtggt ttaaagcggg cgcgattaat gtaacggaga tgccgtcatg
     3421 agctatcaag acatcttgcg gggcctgttg ccccccgtgt cgtatgcccg caatgccccg
     3481 cgtgtgcggg cgcaggcaga aatagacggc gcagcgctgg atgcggtggc ggaatcggct
     3541 caaagcgttg ccgatgccgt cgacccgcgc agcgccggcc aaatgctggc cgattgggag
     3601 cgcgtattag gtttggacgg tacgggcaaa aaccgccagc gccgtgtgtt ggccgtcatg
     3661 gccaagctaa acgaaacagg cggcttgagt attccttatt ttgtgcgttt ggccgaggcg
     3721 gcgggctatc aaatccaaat cgacgaaccg cagccgttcc gcgccggtgt caaccgtgcg
     3781 ggcgaccgtc ttgcgccgca ggaaatcatg tgggtgtggc acgttaacgt gcgcggcggc
     3841 aacaaccgca ttacccgatt ccgcgccggt atctcggcgg cgggcgacag gctgaccgat
     3901 tacagcgatg ccgtgatcga aagcctgttc aaccgcctca agcccgccca caccgctatc
     3961 cgatttacct accgataagg actaaaccat gcaccccatc gaaacccccg acaagacctt
     4021 ccacgacggc gacggcgtgt ccgaattggg caccatcctg cccgcgtggt ggctcaacca
     4081 agtgcaatcc gagctgctgg ccgtgctgac tgcggccggt atccagccgg ataagtcgca
     4141 gcctaatcaa ttactggcag cactaaatag gctggctgta gttatcaccg gcgaccaaac
     4201 cattaccggc caaaaaacct ttactgccca aacccaattc caaagcggca tccatttatc
     4261 cgccaaccag acgaactgga acggcggcca caaagcctac atcggcgcgg atgccgacaa
     4321 cgcccacatc gtcttcggcg acaacaccct gcgcctgcac ggcgcaaaca accgcatttc
     4381 ctacaacaac cacgacatct tccacaaagc caacaaaccg cgttttgccg aagacatcga
     4441 aagcaagccg aacacactgt ccggatacgg catccgcaat ttcaaagtcg aaaccttccg
     4501 gggcgatttg aacaccctca aaacagacgg catctattcc ctgtcgacgg cggtcggcag
     4561 ctccaacctg cccattgaaa acaccgcctg ccatatccaa gtcatcgccg gcacgaaaca
     4621 cggctggtgc aggcagttgg gttatcccgc ctacacgtcc gacgtgtacg aacgccacca
     4681 aacgagcagc gcaaacgaca actggtccgc atggaaaaaa ctcaattcgg acggcatccc
     4741 cgtcggcgcg atcgtatcct ttcccaaagc cgtccgaaac cccgcaggct atctcaaagc
     4801 caacggcacg acctttgcac aaaacacctt ccccgacctc taccgcgccc tgggcaacag
     4861 caaccgcctg cccgatttaa gccgtaccga catcggcatc accgcgtggt ttccgtccga
     4921 ccaaatcccg accggctggc tggcgtttga cgacatccgc acgcgcgtaa ccgaaaccgc
     4981 ttatcccgag ctgtaccgtc tgctgaccgg aaaatacggc agcatccaaa acgtcccgca
     5041 ggcggaagac cgctttatcc gcaacgcggg caacagcttg gcagtcggaa cgaagcagga
     5101 agacgaaatc aaacggcacg tccacaaagt attttcacac tggacaaacc acacagacac
     5161 ggcagccgtc ggttacgaag accgcaacga aaggcagaga agcgcgctcg tatcgacttg
     5221 gacggacgaa aatttaaacg acaacggctt tttaaccccg cgcccggaca gcaaaatggc
     5281 gacaggcggc gacgaaaacc gccccaaagc cttggtttta aaactgtgca tcaaagccgc
     5341 agacaccttg ggcgaagccg tgttctggat aaagtcccac ggcgaaacca tcaacgcagg
     5401 cgcgctggac gcgggcacgc tggcgcaagg tttgcaagac aaagccgacc gcgaccacac
     5461 ccacaccgcc gcccaaatcc aagggctgga cgaaaaaatc agcaccgccg ttgccgcgca
     5521 attcacacgc caaaccatcg gcggcgtgga tattgtcaga ttccccgacg gcacaatgat
     5581 acagaccggc agctacaggt tcacacgaag cggcggcccc atcgaaaacg aagtcgtctt
     5641 ccccgtcgcc tttgccgacg gcaacgtcaa atgcttcgta tccgaacgcc attcgggacg
     5701 cgttaccggc gctcgaaggc aacacaactg gctgtttatc cgcgcaaaaa accacgccgc
     5761 cgccattatt accaactggt acgaaggcag ttgcgactgg atggccatcg gcaaagccgc
     5821 ctcgggaaac gccgccagct ccccgatagg ccccgaaata cctgaaacca acgaagaacc
     5881 gcaaagagag agtggaagaa catcaaccgg accccgaaac cgccgccgcc gagacggctt
     5941 gctcgaggca ctgcaagact agcgggctgt agagatggct gtagagatgg ctgtagagac
     6001 gggctgtaga gatggctgta gagacgggct gtagagatgg gctgtagaga tgggctgtag
     6061 agatgggctg tagagatggg cttcagcccg ccgatccaag caatccgacc gaaaccggcc
     6121 gcgccgccaa tcccccgaaa cctatgcccc gccaatcctg ccactcttcg tcattcccgc
     6181 cgctttcgtc attcccgcga aagcgggaat ccagccccca aacgcggcag gaatctatcg
     6241 gaaaaaacag aaacccccgc caccgtcatt cccgcgcagg cgggaatcca gaccccgacg
     6301 caacaggaat ctatcggaaa aaaacagaaa cccccgccgc cgtcattccc gcgaaagcgg
     6361 gaatccagac cccaaacgcg gcaggaatct atcggaaacg gctgaaaccg aacggactgg
     6421 attcccgcct tcgcgggaat gacggaaagt ggcgggaata acgaaaggcg ggaatgaccg
     6481 cgcaaaaagc cgctgccccc ttcggacggc accggcaaca aaaaaccgca cggccgaaac
     6541 cgcgcgggaa aggatagtcg ggcgcgcccg ataagcagcg gccgcccccg ttatttcaat
     6601 tgggcgacat attggcgcaa aacctcgttg atgcgcgtct gccagccctt gccgccggcg
     6661 cggaattttt cgaccacatc ggcggacagg cgtatggtaa cgagttgttt cggggttttg
     6721 cctgtgtttc gtttttgcat tacgcccttt tcttccaatt gtttttgatg ggaaaaaagc
     6781 acctgtgcca agtcttcggg cagggcttcg gcaatggggc gggcaagcgc aaaatcttcg
     6841 gcggcaagtt cccgcacttc gccgtcagcg tttgttaagg attgacgttg catatttttt
     6901 aacctctctt ttattcgctt tgcgaaaact gatgacacgg atgccgtctt ttatcggcgt
     6961 aaaacagaca atgtgcaggc gttgcgtatc gcctagataa gcagcggcaa cataacgcgg
     7021 ttcggggtaa tcaaagcgga catcgggcac aataacggcc gttgtccagc gtatttgccc
     7081 gactgattca aagggcaaat tccgctcttc gatattgcgt tgattttttt cggagtcaaa
     7141 ttcaatcttc attgcagctt gcagcgtatt ttgtcgttac attataagcg gcaaaaaacc
     7201 aaaatgtaaa tacaaaaaag gaaaccccga aatgaccatc tatttcaaaa acggctttta
     7261 cgacgacacc ttgggcagca tccccgaagg cgcggttgcc gtccgcgccg aagaatacgc
     7321 cgcccttttg gcaggacagg cgcagggcgg gcagattgcc gcagattccg acggccgccc
     7381 cgttttaacc ccgccgcgcc cgtccgaata ccacgaatgg gacggcaaga aatgggaaat
     7441 cggcgaagcc gctgccgccg cccgtttcgc cgaacaaaaa accgccacgg cattccgcct
     7501 cgcggcaaag gcggacgaac tcaaaaacag cctcttggcg ggctatcccc aagtggaaat
     7561 cgacagcttt tacaggcagg aaaaagaagc cctcgcgcgg caggcggaca acaacgcccc
     7621 gaccccgatg ctggcgcaaa tcgccgccgc aaggggcgtg gaattggacg ttttgattga
     7681 aaaagttgtc gaaaaatccg cccgcctggc cgttgccgcc ggcgcgatta tcggaaagcg
     7741 gcagcagctc gaagacaaat tgaacaccat cgaaaccgcg ccaggattgg acgcgctgga
     7801 aaaggaaatc gaagaatgga cgctaaacat cggctgaaaa aatacgttta ccacctgttg
     7861 gtagccatcg accaactgtt caacgcccta accggcggcg cggcggacga aaccctctca
     7921 agccgcacct atcgcggcgc gcggctcgcc caaaagccca aaacccgctg gaaggtttta
     7981 tacaccctga tcaacggcgt atttttcgac cgccaacact gccggcaggc gtatatcagc
     8041 gaactgaaag gcaggcagca cgacgcgcgg ttcaaccaaa gccgcgccgc cggggaaaag
     8101 gggacgcgat gaactacttc ggcatggtag agtttctgcg cctgatggca atggtgcggc
     8161 cgccatttgt cttcttctcc ggcacccgca gcgaactgcc tgcttatctt gacctcgtgg
     8221 cagaactgcg cctgaccgga tgggagcgtt ttgcaggcag ccaaaccttg actgtgagca
     8281 gcacatcaac agcaattcaa gctacgacga ccacctgatt tataagttct gaccgcaagt
     8341 agcgtactac ttttaaaggc ataagataat ccccgtttaa cagaccatta aacggggata
     8401 aatttgtgca aaagctaata caatttccta cgcttcggcg gtgcaaaagc tgccgccaat
     8461 tcgtgcaaaa gctgccgccg ccttacattc cagtgcaatg ccgtctgaaa cttcgctaat
     8521 ctcgggttgc cgcgcgctgt gttgttcttc ggtactcagc aaaaagccgt acagacgctc
     8581 caatcgggcg cggtaggcgg tgaatttgtt gtagaacatc cggaagaaag aaagcgcgtt
     8641 ttgcagtcgc gcgaaggctt ggacggtctg ctggatgtcg ccgattttga tttgtccggc
     8701 aaacaggcgc ggcgcttgca aaataatggg gaagagcttg atgccgttgg tgaacatatc
     8761 attaaagccg ctcaggcaga cgctttgtcg cgcaatacgc cagcggttgc ggataatggc
     8821 tttaaaacgg tcagaaagct ggtcgtgttc gtgtttttcg ccgctgtaaa acgccacgct
     8881 ttcggcgtgg tcgcgcacgc ggataaggga ataacggtag tcgccgttga gtttttcgtt
     8941 ttcatagttg taacgaatca aggggttgcc tatccacatc gcgataaagg tcgccaaaat
     9001 cacaaaaata aagacgagcc aaacaatgcc gcgcggaatg tcgaagccga acacggtcag
     9061 gatgccagcc aagccccaca aaacaacggc aaattccaaa gaggtaacga ccgaattgac
     9121 catgccgcgt acaaattcga tggtcgaagc gatgaaatcc tgcgcgtcct gttggatacg
     9181 ttggtcgatg ttgtccggcg cgtggcggcg catttgcagg cggtagtagt ttttgtcggc
     9241 aagccagcgc gtcgtcagca cttcattgag ctgctccgac catttaatcg ccaagccttg
     9301 atcgaggaag tcgttgacga cattgttaaa cgcccgtatc agcaccacgc ccgcgttcat
     9361 tgcggcaaac atccaaaatg ccgaagcatt tcaaatcctg catcgagtcg taaagccctt
     9421 tggacataaa ggtactcaac acattcagcc gcatttcggt taacaccagc gtaatcatcg
     9481 ccgtaatcag cagcaagact ttgaccgcgc ttttcggtgt cagacaaagc caaagcggtg
     9541 tggaataaag ctcggtttgc catttctgca tgggaaattt cttacggtat caatgccgtc
     9601 tgaaaaagac gggtacagtt gattttttga tgaagtttgg ggaagttttg ccggtcaggg
     9661 tacattgcgt gttaatttat agtggattaa caaaaaccag tacgtcgttg cctcgcctta
     9721 gctcaaagag aacgattctc taaggtgctg aagcaccaag tgagtcggtt ccgtactatt
     9781 tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt taatccacta taccatacaa
     9841 ccacgccgga attaagttta aatttgaata aaaggttcgg gttctgcaaa atacagaacc
     9901 cgaaccttgt tcggatattg aaaccggccg ctcgattttg ggcggtgcgg cttgcaagta
     9961 tcaagattcg catatgccgt ctgaagctcg gagaggttca gacggcatat gcttatttgg
    10021 gctgctcttc gacgaatctc ggacctttca agatgccgtt gtgagaatag ggcgacagca
    10081 ggttgtatgc ggcggttttg gaaacctgat aaccgcggtc ggtcaggctg ttggcaatct
    10141 gattgaccac tgcgctgacc aaagccccca acaggccgct gttgctgttg ttgctgcctt
    10201 cgcggatgct ggccgaaccc gaccacaact cttttccgtt gcgggaatcg accagccgtg
    10261 ctttggcgga tacggtcgtc acgctgtcta aaatttgata tgaagtgccg tattcggtaa
    10321 ccgtaatgta caaaaccgca tcattgccga aaatctgatg cagtttttcc ggccggacgg
    10381 cgtgaatatc ggcggcattg gtcaagccgt tttgtttgaa ggtttcctcc acgactgcgg
    10441 cggggaagac gtaatagccg gcttcggaaa gtggcgcggc ggtcgaagcc agtacacccc
    10501 atgttccgtt gacatcgggc gattcgttca gcggcggaac caccaaaatt gaagccggtt
    10561 tgctttcctt gaatgacgtg tagtcgaaat cgggcgcttt ttgaacttgg caggcagaca
    10621 gcgccaacac ggcggcaagc cctaaaatca aaggtttcat cgcttgcctc ctttacctgt
    10681 tttcatcagg aagtccataa atacgcccga ttcgggaaac agccttttct cttcttcaaa
    10741 ctggcggaac gcgccctctt tgtctcccga acgggaaagc agcagtccca gatgggcgtg
    10801 cgcacccggg gcggcattca tttttttgtt gccggcttcc acaaagtatt tttccatctt
    10861 ttcggtctgc ttgcccaacg aagtgtcgtc gtttttcaaa ccttcataga cggtatcggg
    10921 atagccgccg taataataca gggatttttg cccgttgccg ccgcaggcgg tcagagccaa
    10981 gaccgccgca cacagcgaca aacggctcaa ggttttcgga ttcatcattt ctccttaacg
    11041 gttgggttgc catgcgccgt tgtcaacagc ctgaaccagg ctgttgacgg cttcgcggat
    11101 tgccaagtct aaaactttgc cgttcaaagt cgcatcgtag ccggaagtgc cgccgaaacc
    11161 gatgatttca cggttggaaa gtgcgtattc gcccgcgccc tgtgcggaat agacgatttc
    11221 ggaagtattg acgttgacga tattcagagc cacttttgca taggcgattt gcgatttgcc
    11281 gcgacccaaa atgccgaaga gctgatgatc gccgacatct ctgcgtccga attcggttac
    11341 atcgccggta acgacataat ctgcgccttt caggttatgc gctttgccgg aaatgccgga
    11401 ttcctgtttt aatgcgttca aattggtgcg gttcagtacg ttgaagcggt tggtctgttg
    11461 caggtgcgtt actagaatgg tttttgcctg gctgcccaaa cggtcttccc cgtcggagaa
    11521 aatgcctttt tggaagctgg agcggttgtc gaatgttccg acggaaatcg gggtacgaac
    11581 accgtgatat tgcgtattgt aggaggcgac tttctctacc tcgagactgc gtgaggattc
    11641 ggtcgcacag ccggtcagtg aaacggcagc ggcggcaagg acaacggcgg tggaaacggt
    11701 tttcataaaa tttaccctaa ggtcaagtta aggaaataac gggttatcat tattgtcctt
    11761 atgtaaattt aagtcaaggt gtttgtctgt gcgggacgga tgcgcgcgga aggtacggat
    11821 atttttcaaa cggaatcgcg gcgcgggcgg gatgcctgcc ttttccggat ttagaggtaa
    11881 acgatttcgc cgctccgccc tttgctttcg gtacttgccc accagacaaa tgcgggcagc
    11941 acgtccccgt agcttttgcg ttcgcttttg gcttcgcccg gatgggattt gatgcgttgc
    12001 ggggaattga tgggtccggg gacgaggacg ttggcgcgca ggttgccgaa gcgttcccat
    12061 tcgtcggcgg cgactttgca caggtagttc aacgcggctt tggacgcgcc gaagccgccc
    12121 cagtaggctt tgggtgtttc gccgtggctt tcgccgacga agatgacgga cgcgtcgggc
    12181 gactgcttca gcagcgggaa cagggcgcgg gtcagcccca taggtgcgac ggtgttgatg
    12241 cggtattggt tgacccattc ggcgacggtt tggaaatcca gcggcgagag ggcgtaaaaa
    12301 tagccggcgc agtggacgat gccgtccagt ttgccttgcg tggcttcggc aatggtggcg
    12361 gcgaaatgtt caaattcttt ttcttccgcg ctaataaggt caaagcagat ggcgaatggt
    12421 tcggggtatc cggcttcgac aatcgcgtca tagacttttt ccagtttttt ctgatggcgt
    12481 gccaccagaa tcacggttgc gcctgccgcc gcataggctt tggcgacctg ttcgcccaga
    12541 ccttgcgatg cgccggtaac taagatggtt ttgtcggaca gtgtcgccat actttttcct
    12601 ttttggtggt cggttaaggt attttagcgt tttgccgcac cttgtaaagc gtcccgatac
    12661 cggccggcgt tttcagacgg catcgctcag gctttgcagg caggcttccg ccgacgcgaa
    12721 acctgattgt acggcggctt cgagcgtggc ggggtagtcc gggtggaggt agtcgccggc
    12781 ggggaagatg cggtgccggt gcaaccacga caagtccggc ggcggggcat cggctgcggt
    12841 tgtggcgcgt ttttcggtga tgacgcgcac ggcttcgggt tcgcccaaat gcggaaggat
    12901 gcgtttgagg tcggcgtggg ctttgtccgc ccacgcccgg tttgcaaacg cgccgacgcg
    12961 gtcggaaacg ctgatgacgg cggacacttc gttttcaggc agtccgagcc tgccccggca
    13021 aagcagccat tgcaccgtgc cgtcggcaag gccggtcagc ggggcgggca ggcggacggg
    13081 ttcggcgtag cgcagataga cggtggtgat ggcgtggtag cgaaggtttt gatatgccgt
    13141 ctgaacgtgt tcgggcgtgc cttcgggcag gagcgcggcg gcgtggtagg gcgcggtggc
    13201 ggggacggcg gcatcgaaag cttcgccgtt gacgagcact ttcccgtccg ggagggtgtt
    13261 cagacggcat acgcgcgttt cgaggcggat gtccgcgccg agccgttgaa gctccgccaa
    13321 ggcgggttcg gcgacgattg cgcccaaatc ctgcttgggt agaagatagt cgctgccgga
    13381 ttttttcgtc agcacgccgt cggacaaaac gttgcacaac acgcgcaggc ttgcggtttc
    13441 caaaggcgtg ttgagcgcgc cccaaaccaa gggctgccaa aactgcatca cggcggcacg
    13501 cggcacgttc cgctgtttca gccattgcgc cactgtcgtg tcgggctgtc cgaggcgtgc
    13561 ggacttctgc aaatcggaca tatcggcaag cagtttggct ttgaatgcag tcggtgcacg
    13621 ccgggcaagc agcacgccgc ccaaaatatg cagcggcgcg ggcaggggga gggcgcggaa
    13681 ctgcaaaccg ccgtgcatat gccagtgcag cggtacgcgc aaaaaggcgg cacggggatc
    13741 cgaaccgatg gttttcatca ggcgcaacac gccccggtat gcgccgagca aaatgtgctg
    13801 cccgttgtcc aaaaaaccga aaccgtcggt atttccggcc agtgtgcgcg ccctgccgcc
    13861 cgcctgccgg ccggcttcaa acagggtaac gtcggcgtgc cgcgccaagg tgacggcggc
    13921 ggacagtcct gcccagcctg cgccgatgac ggcgattttc gggcgcggat gcggcgtgtt
    13981 catcatttat tcctccaatg gttgaaaccc cgtttttcgg ggcggcgggg gttgggtttt
    14041 cctgaagggc gggcggtatg cgccttatgc ccgttccggc gtgccggggc gcggtttgaa
    14101 tccgaataac caggttttca gggcaatgcg ttttttgcgc ggcgaaggga gggcgatttt
    14161 gtatttgagg acgttttgcg cgccgtcccg gtcgatttcg ttcaatagct cgtaataaac
    14221 cgccgccata accagtccga ctttttgggc ttttttatcg gcagccggca gcagcgatac
    14281 ggcttcgcgg taggtttcac gggcgcgttt gatttggaac gccatcaatt cggcaaaatt
    14341 gcccgtcggg ctgcattgca aaatcacgct tgcggggaca tcaaaccgct gcatttcctc
    14401 catcggcagg taaatccgtc ccctgcgcgc gtcttcgccg acatcgcgga tgatgttggt
    14461 cagttgcagc gcaagtccca tcttgtcggc gtattccagc gttttgccgt ctgaaaaccc
    14521 caaaatccgc gcaatcaggc agccgactac acctgcaaca cggcggcagt acagtttcaa
    14581 ttcttcaaaa ctgccgtaac gcgcccgaac caaatccatc tgcatcccgt cgattaaggc
    14641 ttccagttca tatttcggca gcttgaaggt ttccttaacc tgccgcaagg cctgattgac
    14701 ggggtgttcc ggcatcgcgc cgccgaatac cttgtccaaa tcgccgcgcc accagttcaa
    14761 tgtcgcctgt gcaacatcgg ggttggaaca ttcgtcaacc acatcgtcca attcgcggca
    14821 aaaagcatat aaaaccgtta ccgcatcccg tttttcctga gtcaggaaac ggaagcccga
    14881 caaaaaactg gagcggcttt cttctgcttt ttggtggcaa tagtcgagtc ctttcacgat
    14941 ttatattcct aatgatggga gggaaaggcg gattttatcg gcatttggcg gtagagggca
    15001 atttcggcgg cacgacccaa tccttagcgg tttgctcaac tatcggcgcg aattctgtta
    15061 aaatgccgac ttccttcctt tacacaccgc accgacaggc agaatttatg gctcttttgc
    15121 agatttcaga accgggtatg tccgccgccc cgcaccgaca ccgtttggcg gcaggcatcg
    15181 atttgggtac gaccaacagc ttggtcgcca ccgtccgcag cggcagtgcc gcctgtctgc
    15241 ccgatgccga agggcgcgtt accctgcctt ccgtcgtccg ctatctggaa aacggcggca
    15301 ttgaagtcgg caaaaccgcc ctgtccgccc aaaaaaccga cccgctgaac accgtcagct
    15361 ccgccaaacg ccttatcggg cggacgcttg ccgatctgca tcaaaatacg cattacctgc
    15421 cttaccgttt cggtgacaat caacgcgtta tcgaactgca tacgctgcag ggggtgaaaa
    15481 cgcctgtcga agtgtcggcg gaaatcctca aaacccttaa atcgcgcgcc gaagaaacct
    15541 tgggcggcga tttggtcggc gtggtgatta ccgttcccgc ctatttcgac gatgcgcaac
    15601 gccaggccac taaagatgcc gcgcgtctgg cgggtttgaa cgtattgcgc ctgctcaacg
    15661 aacctaccgc cgccgcaatc gcctacgggc tggacaacgc ctcggaaggt acgtttgtcg
    15721 tgtacgactt gggcggcggt acattcgacg tatccgtatt gcaactgacc aaaggcctgt
    15781 ttgaagtcaa agccaccggc ggcaacagcg cgttgggcgg cgacgatttc gaccaccggc
    15841 tgttctgccg cctgctcgaa caaaacggac tctcccaact caacgaacaa gacagccaac
    15901 tcctgctctc gctcgtccgc gccgccaaag aacaattaac cacgcaaacc gaagcgcgca
    15961 ttcaggcgac gctttcagac ggcatggcaa tcgacacaag catcagtcgc gccgagttcc
    16021 acaacctgac gcagcatttg gtgatgaaaa cgctcgagcc ggtcaaacag gcgttgaaag
    16081 atgccggtgt cggtaaaaac gaagtcaaag gcgtgattat ggtcggcggt tcgacccgta
    16141 tgctgcacgt tcaacaggca gtcgccacct tcttcggaca aaccccgctg aacaacctca
    16201 atcccgacga agtcgtcgcg ctcggcgccg ccatacaggc aaacgtcctc gcaggcaaca
    16261 aaaccgacgg cgaatggctg ctgctggacg ttacaccttt gtcgctcggt ttggaaacct
    16321 acggcggctt ggcggaaaaa atcatcccgc gcaattccac catccccacc gcgcgcgcgc
    16381 aggactttac caccttcaaa gacggtcaga ccgcgatgac gatacacgtc gtacaaggcg
    16441 aacgcgaact ggttgccgac tgccgcagcc ttgccaaatt cactctgcgc ggcattccgc
    16501 ctatggcggc gggtgcggcg cgtatccgcg taaccttcca aatcgatgcg gacgggctgc
    16561 tgtccgtttc cgcccaagag caaagcaccg gcgtacacgc gcaaatcgaa gtcaaacctt
    16621 cctacggctt ggacgacgac accatcaccc aaatgctcaa agacagcatg agcaatgccg
    16681 ccgaagatat ggcggcacgc gcccgtgccg aagccgtggt cgaagccgaa agcctgaccg
    16741 atgccgtcaa cgccgccctc gagttggaca gcgatttgct ggatgccgaa gaattgcaac
    16801 agattcggca aggcatcgcc gatttgcaag gccgtctgaa agacggaaaa gccgaagaca
    16861 tccgtgccgc cgccgccaaa ctcggcagta ttaccgacaa tttcgccgcc aaacgcatga
    16921 accgcaacat ccaacgcgcg ttgacaggcc agagtgtcga taatatttga tacttaaacg
    16981 gtttcagacg gcatagatat aacctgatgc cgtctgaagg ctcgaaaaca cttgaaaaac
    17041 atcgatatgg aaaagtcagg cattgtctat tcgatgaaaa ccgtcatcaa gggcgtgtat
    17101 agtgaattaa atttaaaccg gtacagcgtt ggctcgcctt agctcaaaga gaacgattcc
    17161 ctaaggtgct gaagcaccaa gtgaatcggt tccgtactat ttgtactgtc tgcggctcgc
    17221 cgccttgtcc tgatttttgt taattcacta tataaaatcg gcatttcgga ggtaagcaat
    17281 tttgaaggca gaatgcgcca tttggaaaac aacggctatg cgaacgttgc cggattggaa
    17341 cgcatcctcg ccgtcaaaac cgacaattac aaagaaaaag aaaacctgct ccatgaaatt
    17401 ttcagcaaaa gcaggatagg cgataccgaa ttgttcgccg tggacgaaaa ccttgtaaaa
    17461 cgtttgtttt tatcgcttcg cggcgaaatc gtgtttccga aaaacgaaac ggcggaatcg
    17521 gaatttgaaa aaagcgtcca cgaacgcagg caggaaggga atgccgggtc cggccgcaaa
    17581 caactgcttg atttggtacg gcgcggacac cgggaatacc cttacgcgct gccccggctt
    17641 ttggcgggcg cggcatccta caagccgaaa aaatcgaaaa tccgcctttt taaagaagca
    17701 tatttcggca aaagcggcac gaggctgacc gacgaaattg cagacggcat ccatatttac
    17761 acctgttttt cgcgggcgga tttggaaaaa gcctattccg aatatttgga acttttcaaa
    17821 tccgaatcgg atgccgaagg cagaaagccg cagtaaggtg caaacagata ccgtacacgt
    17881 tgaggagcag atatgatggg cgattccgtc atttattatg tagaacaggc agacaaaccg
    17941 gtaaaccgtg ccggcgaacg cgcccgtaaa acattcaaat atttttggcg cgagcttttt
    18001 tgggaacgcc gccgcatcat tcccgcctta gattttgcca tggtcaaagc cccttttttc
    18061 caagacggcg aagacggcga aatttgcgaa catatgtgga tcgacgatat ttattttgac
    18121 ggtctttaca tttatggcgt gctgaacaat gaacccggcg aactgaccaa tgtcgaacaa
    18181 ggcgaaagcg tttgcgttcc ggttgacgac atcagcgact ggatgttcgt gtgcaacggc
    18241 attccctacg gcggctttac catacaggca atgcgtggac agatgacgga agaggagcgt
    18301 accgaacacg atgccgcatg gggaatcgat ttcggcgatc ccgggcaggt attgctggtg
    18361 tatgaagaaa aagaacatcc cgaaaatctg gaagagcatc cgatgtgccg gaactgtatt
    18421 gacgattttc ggcaacagtt gtcccaaaat ccggattttc tgcatgagca ggacgaagac
    18481 ggctatacgc cgcttcatca tgaagccatc gcaggaaatg cacttatggt tcaagccatg
    18541 cttgaatacg gcgcaaatcc tgcctcaaag acatcggaag gctataccgc cctcgatttt
    18601 gcccgcctgg cgggctggca aaatgttgcc gacctgctcg aaccgcgaca ttaggcggac
    18661 agttttccga aaacgaacac aaacactttt tacagaaaga caataaaaat gcccaaaatc
    18721 accgtacttc cacacacgac attatgcccc gaaggcgcgg ttatcgaaga cgcgcccgaa
    18781 ggcaaaaccg tccttgacgt gctgctcgac catgatatcg aagtcgatca cgcctgcgaa
    18841 aaatcctgcg cctgcacaac ctgccacgtg attatccgca aaggtttcga cagcctggaa
    18901 gagccgaccg aattggaaga agacctgctc gatcaggctt ggggtttgga agccgattcg
    18961 cgcctgagtt gtcaggcggt tgtcgccggc gaggatttga ttgtggaaat ccccaaatac
    19021 accatcaacc acgcgcgaga agaacactga aaacaggccg tctgaagccg gcacgcttca
    19081 gacggcattg ttgcgcggat aaggcgcaat cgcccgaaaa caggcgttcg tacaggcgga
    19141 actttcgatt ctatagtgaa ttaacaaaaa tcaggacaag gcggcgagcc gcagacagta
    19201 ccaatagtac ggcaaggcga ggcaacgctg taccggttta aatttaattc actatatatt
    19261 gatttttatc ggttttctga cggaacggat tggtgcggca tccgaggcgg attactcggg
    19321 cgcgatgcac gggtatttat cggttttgca gccggaaaaa ccgccggcgg gttactttca
    19381 gggcggcggt cgggcggtat gccggatagc gttccacttt cgcttctata ttgattttga
    19441 ttggttttct gacggaatga tccgatgcgg catccgggac ggcttgtgtt tcttcctgcc
    19501 cgcctgccgg atattcctgt acttgcacga aaccgaaaga gacggcggcg cggcggacaa
    19561 gctcgagata gcgtccttca agctccaggc aggcggcgga cacgctttct accgtaaccg
    19621 tgaaaccgcc gcccgacccg gcaaggcgtt ccgcaatctg cgtgtagatg agccaacgtt
    19681 gggcgcgcaa cattgccgca tcgcaaccgg cagccgcttt atccaaggca atcaggccgc
    19741 gccggttttg gtggtgaagg caaaccgtta ctctggagag gatatgttgc ccgtccaata
    19801 tttgatacag tttgcgtatc agcagaatca ggcggtcaaa ctcctccatc tccgacaggg
    19861 aatcaggatg gtcggaagcg gtataaacca gtttggacaa ttttgcggca aacatattgt
    19921 tcatcaatct tccttgtcgg ttgaaacccc gctcttcggg gcggtagaat cagatttgtt
    19981 tgggagggac aactcctccc agatcaggac gacacatagg ctggtgcttg atgtgttgtc
    20041 cggcgagttg aaacattcag ccatcctcaa ggtgcggcag ttttgccgaa acatattcta
    20101 cacggcttca atgccggacg ataaaaggaa attcatatga aatggaccga cacccggcgt
    20161 atcgccgaag aactctatga cctgcacggc gaaaccatcg atcccagaac cgtgcgcttt
    20221 acccaactgc gcgacctgat tatggcattg cccgaatttg atgacgatcc cgcccgttgc
    20281 ggcgaacgta ttctcgaagc cgtgcagcag gcatggatag acgaggcaga ataagtttcg
    20341 ggaatgccgt ctgaaatacg gcggtacgcg gttcgtgctt ctgtttgcag tgggaacggt
    20401 tttaccagtc tccttttttc agcctatcca gttggcggcg gtcgcgcttg gtcggtctgc
    20461 cgtcgggatg ggcggaagtg atgcggctga attggtcgag ctgtttgcgc tcttccctca
    20521 atgctgccgt ttttgcgtct tcttcataca gaagccgcgc ctcggatgcc gggcggcgtt
    20581 ggtggttcaa acctttaacc ttgattttat agggaaggga attgagcgtc aggtcgataa
    20641 tatcgccgat gtctatggtt ttactgtttt tgaccttcga gccgtttact tgaaccctac
    20701 ccagttcgat gtgcttttgc gcaagggaac gggtcttgaa aaaacgtgcc gcccaaagcc
    20761 atttgtccag ccgcatggcg gaagaatcgt gcttgtcttt catacgattt tgtttgaaat
    20821 aattgaattt gtttcgagtt tagcataaga tacgccgcct ataactagta tatatgcact
    20881 aatccactgt tttccatgct gtccgaacac aaaaagaggg tgtggaaaag ccgttttgga
    20941 caataaatta actgcggaat atgcacaaat agcgtatgat agcggcagaa tctgttgatg
    21001 agagcttcat tctatgaaac ctgttttttt ggattttgaa caacccatag ccgaactgac
    21061 caacaaaatc gatgagctgc gtttcgtcca agacgagtct gccgtcgata tttcggacga
    21121 aatacaccgt ttgcagaaaa aaagcaacga cctgaccaaa tcgattttca gcaaactcac
    21181 acccgcccaa atttcacagg tttcccggca tccgcagcgt ccctatactt tggattacat
    21241 tgaggcactg tttaccgatt ttgaagaact gcacggcgac cgccactttg ccgacgatta
    21301 tgcgattgtc ggcggattgg cgcgtttcaa cggacaaagc gtgatggtcg tcgggcatca
    21361 gaaagggcgc gacaccaaag aaaaaatccg ccgcaacttc ggtatgcccc gtcctgaagg
    21421 ctaccgcaaa gccctgcgcc tgatgaagac ggcagaaaaa ttcggcttgc ccgtcatgac
    21481 ctttatcgat acgccgggcg cgtatcccgg catcggcgcg gaagaacgcg gacagtcgga
    21541 agccatcggc aaaaacctgt acgaactgac gcgcctgcac gtccccgttt tgtgtaccgt
    21601 catcggcgaa gggggctcgg gcggtgcgtt ggcggtcgcc gtaggcgatt acgtcaatat
    21661 gctgcaatat tcgacctatt ctgttatctc ccccgaaggc tgcgcttcga ttttgtggaa
    21721 aaccgccgaa aaggcggcgg atgcggctca ggctttgggc attactgctg accgcctgca
    21781 aaagctggac ttggtcgata ccgtcatcaa agaaccattg ggcggcgcgc atcgggattt
    21841 cgggcaaacc atgaaaaacg taaaagccgt tttggaaaaa caactgcacg aagcgcaaag
    21901 catcccgctt gccgatttgc tttcgcgccg tttcgaccgc attatggctt acggcaaatt
    21961 ttcggaacaa taattcaggt agaacaagca gcaagcagtt tgtctgaaac tgcttgcttt
    22021 ttctttatcg ggacggaacc gtgctgactt tagatgcgtt tgagcaatgc ttgaaggatt
    22081 gttttcctca aggtctgaat ggaaaaaaaa cagcggtggc attaagcggc ggcttggatt
    22141 ccgtcgtttt gctgcatctg cttgtccgcg ccgggaaaaa aggcggtttt attccggatg
    22201 cattgcatat ccatcacggc ttgagtcccc gtgccgacga ttgggcggat ttctgccaaa
    22261 actattgcga tatgctcggg gtggggctgg aaacggttaa gatctgcgtg gaaaaaaacg
    22321 gtttgggcat cgaggcggcg gcgcggcaaa agcgttatgc cgcgtttgcc gaaaaaggct
    22381 ttgacgtttt ggcgttggcg caccacaggg acgatcaaat cgaaaccttt atgctggcgg
    22441 tcgcgcgcgg cggcggtttg cgcgctttgg cggctatgcc cgccgtccgc ccttttgggg
    22501 aaaaaggcat catttggcgg cccttgctgc ctttttcgcg ccaagatata tgggattatg
    22561 cccaaaaaca cggtttgccg aatatcgagg acgaaagcaa tagcgatacg gcttatttgc
    22621 gaaaccgctt ccggcaccgt attttgcccg aactttcggc gcagattccc catttcgggc
    22681 ggcatctgtt gaacaatgtc cgtgccttgc aggaggattt ggctttgttg gacgaggtcg
    22741 tcgttcaaga ctgccgttgg gtttgcggag ccggttattt cgatacggcg cggtggctga
    22801 cgttttcccc gcgccggaaa acccatattt tgcggcattt tttgaaggaa aacggcattc
    22861 ccgtgccgaa tcagaatgcc cttgccgaca ttgcccgggt tttgacggag gcaaaaaccg
    22921 gacgttggaa cgtgcaaggc tttgaattgc atcattatgc aggcaggctg tttgtgttcc
    22981 gaccggaaaa aacggataaa ctgcggtttt tgaaagacag gcagataagc ggaaatttaa
    23041 gggaaatatt ggcggggcag ggatttgttt taaaacgtca tccgttcgga cttcccgggc
    23101 atcttttgga gcaggacgga atcttgcgga cggttgcggc atcggacagg ctggctgtcg
    23161 gcggtatcca taaggatgtg aagaaaatcc ttcaggaaaa gcgggttttg cccgcgctgc
    23221 gcccgttttg gccggtcgtc gccgacaagg acaaccgtcc gctggcgttg gcaaattacg
    23281 gtacggattt tcaaatctcg gtttcagacg gcattttgcc cgtccatccg gattttcccg
    23341 ttttattttg atggaatcgg aatcgccgtt acctattata tattcttgaa aaggcggtaa
    23401 agtgaagcat atcagttcgg caaacaatga acacatcaga cacctgcacc gcctgttgtc
    23461 tcaagggaag ttcagacggc aatacgccca aaccgttttg gagggcgtgc acctgcttca
    23521 ggttttcctg caatccggcg gaaggccagt cggggtatat attcccgaag cgaaaatgcc
    23581 gtctgaagaa gtccgtaaat tgacggcggt tttgccggaa gacggggttt tttccgtttc
    23641 agacggcata ttgaagaaaa tcagcagcct gacttgcgcg gatgatgtgc ttgcgctgat
    23701 tgatattccg gatgcgggtg ctttgccggc cggcggcgac tgtgtggttt tggacggcgt
    23761 gcaagatccg ggcaatgtcg gcacggtgtt gcggagcgcg gcggcggcag gaatcggcgc
    23821 ggtcgttttg ggcaaaggtt gtgcggacgc gtggtcgccc aaagtgctgc gagccggaat
    23881 gggcgcgcat ttcttgtcgg agatttatcc gcaggcggat ttggaaatat ggttggcgcg
    23941 ttatgaagac cgtgtgtttg ccaccgcgtt gcgtgaggaa aaacaggcgg ttctgtataa
    24001 cgaagatttg cgcaaaccga cagcctgggt gtttggcaac gaaggcgcgg gggtcggtaa
    24061 agcagtttta gacagggcgg acaagtgtgt caggataccg atgcacgatg caaccgagtc
    24121 tttaaatgtc gcgatggcgg cgacaatctg cctgtttgaa caaatgcgcc aacgggcggc
    24181 gtattgagga aaagaaatgc cgtctgaaaa aatctattac ggcgtattga ttttcttatg
    24241 tatcgcttct atgctgctgt cgccgttttt ttatgcgggt gctttgaagc ccaagaaggc
    24301 ggcattgcgg aaggacgggc agtggaaact catcttattg tccaataccg tggcggcggc
    24361 ggttttggct tgggtgtggt ggaaatggtt ttgacagata ttgctcaaaa tcgtgctaat
    24421 ggaatccgaa caaataaaga atttggtaaa aaatttgtta aatcaacgaa ttaaagtttt
    24481 gtggaaaaca aaacagctct aagcaaatag ggcgtttgtc ggtaaatacg gaagagttgc
    24541 ggcattatcg ggcatcttta acaagtagtg ccgtcttgac aggcaatcgg tttttatggg
    24601 cagcttgcaa aatcgcggat ataaaattgc aaatcggtta aagtgtgggg acgctatgaa
    24661 aaattgcgaa tttttttatg acccgacaag ggcaatctat gatagcggtg cagattattt
    24721 aaccagagaa aaacatagat tcgtcgtaat tgcaaatagt gcttgggggc tattgcttaa
    24781 tttatcttgt tattatgacg aggttttgga aaagcggaaa ataccgttcg gcaaacagga
    24841 aattgatgac gatatggaca aagtgtccgc ccataagcgg aagtttaaag atatttctga
    24901 aatcaaagta ggggatggtt gggaataccc gttcaattat gagcagggaa tgaaagaatt
    24961 agatgaagtg ctattgaaat acattccctt ttttgaagaa gaacgataaa ggaggttgat
    25021 atgcgcgtat ctaaaataat tggaagtatg ttgcttgtta cagcggttca gaccgtattt
    25081 tcggcaaatg tttacgcgtg ccgccataat ggtaaaacca gttacagcca aactccggga
    25141 aaacattgta ccaacgcggg tttggggcgg gatcgggtgt acagttcggt tagaccggca
    25201 gtaaaagaca gggcggaaga cgcaggggtc ggcgattatt cggacacggt gagggacgaa
    25261 cacgtccaaa atccgagaga gaatacgcat aaggatgctt cgaataccgg tgcaaagacg
    25321 cattgattga aaccgaatca gccatgacgg cgttgaataa tagaatttga acgattaggg
    25381 aactctgatg aaacacatcc acattatcgg tatcggcggc acgtttatgg gtgggattgc
    25441 cgccattgcc aaagaagcag ggtttgaagt cagcggttgc gatgcgaaga tgtatccgcc
    25501 gatgagcacc cagctcgaag ccttgggcat aggcgtacac gaaggcttcg acgcggcgca
    25561 gttggaggaa tttaaagccg acgtttacgt catcggcaat gtcgccaagc gcgggatgga
    25621 tgtggttgag gcgattttga accgcgggct gccttatatt tccggtccgc aatggctggc
    25681 tgaaaacgtg ctgcaccatc attgggtact cggtgtggcg gggacgcacg gtaaaacgac
    25741 caccgcgtcc atgctcgcat gggttttgga atatgccgga ctcgcgccgg gcttcctcat
    25801 cggcggcgta ccggaaaact tcagcgtttc cgcccgcctg ccgcaaacgc cgcgccaaga
    25861 cccgaacagc caatctccgt ttttcgttat cgaagccgac gaatatgaca ccgcgttttt
    25921 cgacaaacgc tccaaattcg tgcattaccg tccgcgtacc gccgtgttga acaatctaga
    25981 attcgaccac gccgacatct ttgccgattt gggcgcgata cagacccagt tccaccacct
    26041 cgtgcgcacc gtgccgtctg aaggcttaat catctgtaac ggacagcagc aaagcttgca
    26101 ggatactttg gacaaaggct gctggacgcc ggtggaaaaa ttcggcaccg aacacggctg
    26161 gcaggtcggc gaagtcaatg ccgacggctc gttcgacgtg ttgcttgacg gcaaaacggc
    26221 cggacgcgtc aaatgggatt tgatgggcag gcacaaccgc atgaacgcgc tcgccgtcat
    26281 tgccgccgcg cgtcatgtcg gtgtcgatat tcagaccgcc tgcgaagcct tgggcgcgtt
    26341 taaaaacgtc aaacgccgca tggaaatcaa aggcacggca aacggtatca ccgtttacga
    26401 cgacttcgcc caccatccga ccgctatcga aaccacgatt caaggtttgc gccagcgcgt
    26461 cggcggcgcg cgcatcctcg ccgtcctcga accgcgttcc aatacgatga agctgggtac
    26521 gatgaaagcc gccctgcccg caagcctcaa agaagccgac caagtgttct gctacgccgg
    26581 cggcgcggac tgggacgttg ccgaagccct cgcgcctttg ggcggcaggc tgcacgtcgg
    26641 caaagacttc gatgccttcg ttgccgaaat cgtgaaaaac gccgaagcag gcgaccatat
    26701 tttggtgatg agcaacggcg gtttcggcgg aatacacacc aaactgctgg acgctttgag
    26761 atagcccggg cgatgccgtc tgaaagtcct tcagacggca tcgcccggct gcgcggcaca
    26821 aaggcggaaa aaccgtttgc cccgtatttt caaacgcgtt acacttgccg ccgctgtttt
    26881 cagccatttg attacccgca accgccgtca ttgcgccggc ggtctgcacg tcagcgtcat
    26941 tgcgccgctg taaatacgaa agaacacatt atgaccgtat cccccgtcgc cttgcgccgc
    27001 aagaccgagt gcaagccgca tcccaccgcg cgctattgga aaaaatgcga tgtcgaagcc
    27061 ctgttcggac ttcccttcct cgacctcatt taccaagccg ccgaaatcca ccgccaaaat
    27121 ttcaacccgc gcgaaatcca gctttccacg ctgttgtcca tcaaaaccgg cggttgtccc
    27181 gaagattgcg cctactgtcc gcaatcggcg caccacaaca ccaatctggg caaagagcag
    27241 atgatggatg tggatgaaat cgtcgaaaaa gccaaaatcg ccaaatcgcg cggcgcaagc
    27301 cggttttgta tgggcgcggc atggcgcggc cccaaaccca aagacgtgga gacggtttcc
    27361 gcaatcatca aagccgtcaa gggcttgggt atggaaacct gcggcacgtt cggtatgctc
    27421 gaagaaggta tggcggaaga cttgaaagag gcgggcttgg attattacaa ccacaacctc
    27481 gacaccgacc ccgaccgcta caacgacatc atccatacac gccggcatga agaccgtatg
    27541 gacaccttgg gcaaagtccg caacgccggt ttgaaagtct gctgcggcgg catcgtcggg
    27601 atgaacgaaa ctcgtgccga acgcgccggg ctgattgcca gcctcgccaa cctcgacccg
    27661 cagcccgaaa gcgtgccgat taaccggttg gtcaaagtgg aaggcacgcc gcttgacgat
    27721 gccgaagatt tggactggac ggaatttgtt cgcaccatcg ccgtggcgcg gattacgatg
    27781 ccgcaaagtt atgtccggct gtcggcaggg cgcagcaata tgccggaagc gatgcaggcg
    27841 atgtgcttta tggcgggcgc gaattcgatt ttttacggcg acaagctgtt gaccacaggc
    27901 aatccggacg aagacggcga cagaatcctg atggaaaagc ttaacctgta tcccttacag
    27961 tttgagctgg aaggcgaggt cgccgaagtg gaaaaagcct cggggattaa agtggattat
    28021 tgacgattga aaaatgccgt ctgaaacccg gaaaaaggct ttcagacggc atttgtccgg
    28081 acggcatttc caatatcttt ttaccggcgc gtgatgctgc cgtcgggcga gacatccagt
    28141 ccgttcccgt tggggaagtc gctgctgttc agataaataa tccgcccgat aacggttttt
    28201 cagggttgat tttggggatt ttatcgtcgg cttgggtaat cgggatgatg gttccggaaa
    28261 taaatttacc ctgtttgtcg gtgataattt taaaaatggg ggcgatgccg ctgatgccgg
    28321 aggtgttgat tgcgccgtag gtggcaaagt tgccgccgct gtaggagatg aagcggtcgc
    28381 ggtaaagttc gacggcgcga gtaacgtgcg gcctctgccc gaatacgaca tccgcgccgg
    28441 aatcgacggc aagccgcgca aactcaacga cgttacccct gttttcccca tagaagattt
    28501 cggtatcgaa cggcaggtgt tccgcctgtt tcccttccgc gccgccgtgg aacatcacaa
    28561 tgacgatgtc ggctttctgt ttggttttgg taatccgttt tctaactttg gcataatcgt
    28621 tcagtttgac ggcggcaagg ttgggggcga aggagacgaa gccggatctt acgccgtttt
    28681 tcttcaggat ggcggtttca aacctgtttt cgatgcccga atatttgatg ttcaattcgt
    28741 caaggttcgc cctttatgcc gtttccgcac cgcaaaccgc cggggtcaat ccctcggcgc
    28801 atcctgccgg aacggaatcc ccgtgctgcc gattgacggt tcaaaccctg cgcccgtttc
    28861 aaataccggc gatgtggacg gacaggatgc gcctgacgaa aagacagccg ataccgtttc
    28921 cattatcggc gtgggcgaca ttatgctcgg cagcaattat ccggtcgatt acctgcccga
    28981 taccaatatt ctgaaaaacg tcgaatctgc cttgcaagac gcggacatta ccgtcggcaa
    29041 cctcgaaggc acgctgtttg acgaaggcgg tacgccgaaa aaatgtgcaa ccccccaaaa
    29101 tatgctatgc attccgaacg ccctccgcat acgggcaata ccttgccgac gcgggattcg
    29161 actacctcag cttcgccaac aaccacagca acgacttcgg cgcgcaaggc atcacggcaa
    29221 cggcggcgag cggcagctct tttacatact cgatcgcgct aaagccgctg ccgataacga
    29281 ggccaaaatt gcggaaaata ccgccatcgc ccagataaat ttgtccatca tcagaccttt
    29341 actgttcaga cgagacagca tttgccgcac gttttggggc ttatctttcg atttgcgcta
    29401 cgtcgcgcac cgcgcctttg tcggcggaag tcgccatcgc gccgtaagct cttaatgcgg
    29461 cggagacgta gcggtcgcgg tttttaggct tccatgcttt gctgccgcgc gcttccattt
    29521 cggcacggcg tgcggcaagc tcttcatcgg aaatggcaag gtggatgctg cggttgggga
    29581 tgtcgatttc gacggtatcg ccttcgtgta ccaaaccgat cgcgccacct tccgccgctt
    29641 cgggcgaggc gtgtccgatg gacaaacctg atgtgccgcc ggagaagcgt ccgtcggtta
    29701 agagagcgca ggctttgccg aggcctttag atttcaggta ggaagtcgga tacagcattt
    29761 cctgcatgcc cgggccgcct ttcgggcctt cgtagcggat gatgacgatg tcgccagcga
    29821 cgatttggtt gcccaaaatg ccttctactg cgtcttcttg gctttcaaac acgcgggcgc
    29881 ggccggtgaa tttgaggatg ctctcgtcca cgcctgcggt ttttaccacg cagccgcgct
    29941 cggcgatgtt gccgaacaag accgccaaac cgccgtcttg cgagtaggcg tgtgccacgt
    30001 cgcggataca gcctttttcg cggtcgaggt cgagggtttt ccacatacgg ttttgcgaga
    30061 acgcttgggt ggtgcgtacg ccgccgggcg cggctttgaa gcgttcgatg gcgtgggtgt
    30121 tttcagggtt ggttacgtcc catttttcaa tcgcgtcttt cagcgtcggc gcgtggatag
    30181 tgtgcacgtc ggtgtgcagc ttgcccgctt tgtccaattc tttcaggatg gcgaaaatgc
    30241 cgccggcgcg gtgcacgtct tccatatagt agtcgtggtt gttgggtgcg gttttgcaga
    30301 tgcagggcac aacgcggctt aagcggtcaa tgtcggccat tttgaaatcg acaccggctt
    30361 cgttggcaac ggccaacagg tgcaaaatgg tattggtgct gccgcccatc gcaatgtcca
    30421 tcgtcatagc gttttcaaac gcttttttgg tggcaatgct gcgcggtaac acggtttcat
    30481 cgttttgctc gtaatagcgt ttggtgattt cgacaatcat acggccggct tcgaggaaca
    30541 gttctttgcg gccgacgtgg gtggcgaggt aagaaccgtt gcccggcagg gacagcccga
    30601 gtgcttcggt caggcagttc atcgagtttg ccgtaaacat acccgaacac gagccgcagg
    30661 tcgggcaggc gttttgttcg acttcttcga cttgccggtt gctgacattg tcgtctgccg
    30721 attcgatcat cgcgtcaatc aagtccaagc ggcgttctgg ttggatgttt gccacgccga
    30781 taactttacc tgcttccatc gggccgccgg agacgaagat ggtggggatg ttcaggcgca
    30841 tcgcggcaat cagcatgccc ggggtgattt tgtcgcagtt ggaaatgcac accagcgcgt
    30901 cggcgcagtg ggcgttgacc atatattcga tagagtcggc aatcaaatcg cggctgggca
    30961 gggagtacag catgccgctg tgtcccatag cgatgccgtc gtcgatggcg atggtgttga
    31021 attctttggc gattgcgccg gctttttcga tttcgcgggc aaccagctgg cccatattgt
    31081 gcaggtggac atggccgggc acgaattggg tgaaggagtt ggcaacggcg atgatgggct
    31141 tgccgaagtc ggtttccatc acgccggtgg cgcgccacaa tgcacgtgcg cccgccatat
    31201 tgcggccgtg ggtggaggtt ttggagcggt attctggcat agtgtgtttc cttgtctgct
    31261 atgccgtctg aacggcaaaa ggctttcaga cggcattttt gttgtgtttt gaaatgaaaa
    31321 aaaaggtatt ttataccaag tatcggaatt ttgcggaatg gaaacgcgtg cggcaaaaaa
    31381 ggacatcccc cgcaggaatg cgggggacgg tttcaggcgc gcgcaatcgg gacggctttg
    31441 gatgcgtccc aaaaatcgac gggcgcatcc aacacgggtt tgacgatgcc cgtccgtatg
    31501 gcgaaatcgc cgaaaccttc gtcggtatcg cgtcctgccg cccatttgcc gatcaggtcg
    31561 tctaattcgg aaaggatttc cggcagggta atgttttctt tgtagaggcg ggggatgcgt
    31621 acgccttcgc ggtcgccgcc gatgtggagg ttgtagcgtc cgacggcttt gccgaccagt
    31681 ccgatttccg ccaacatcgc ccgtccgcag ccgttcgggc agccggtaat gcgggtaacg
    31741 atgtagtcgt ccgacgtgcc gtgtttcgcc ataatcttat ccagctcgcc gatgaagtcc
    31801 ggcagcacgc gttcggcttc cgccattgcc agcgggcagg tcggaaagga aacgcaggac
    31861 atcgcatttt cacgcagctt gctgacatcg ttgcggatta atccgtatgt tcgggcaaat
    31921 tcttcgattt ttgctttgtc tgcttcggcg acatttgcca cgatgaggtt ttggttggcg
    31981 gtgatgcgga aatcgccttt gtggattttg gcgatttcca acacgccggt cagaagctgt
    32041 ttcccgcctt cgtcaaccaa acgcccgctt tcgatgaaaa gggttaaatg ccagttgccg
    32101 tctatgcctt tcacccagcc gatgcggtcg ccgcgcccgg taaatttgaa cgggcgtacg
    32161 ggttcgaacg gcatacccat acggcgttcg acttccgcgc ggaagttgtc caagcccata
    32221 ttttgaatgg tgtagcgggt gcgggcgttt ttgcggtcgc tgcggttgcc gaagtcgcgc
    32281 tgtgtggtta ccaccgcttc ggcggccttc agcgcgtgtt ccggaggtac gaaacccagt
    32341 tccagtgaaa tgttcggata ggttttggtg ttgccgtgtt ccattgaaag cccgccgcct
    32401 gccaaaacat taaaaccggc aagctgtccg ttgccgtctg aaacggcgac gaaatccaaa
    32461 tcgttgccgt agcagtccac atcgttcaag ggcgggatga cgactgcggt tttgaatttg
    32521 cgcggcagat aggttttgcc caaaatcggc tcgtcttctt gaaggaagtc gtcagaactt
    32581 tgaacttttt tgccgtccac ccatacatcc agataaccgc gcgtgcgcgg cagcaggtgt
    32641 tcggaaatct ttttcgcgta ttcgtaagcc tgccggtgca gttcggactc gatcgggttg
    32701 gacgtgcaaa gcacgttgcg gttcatatcc gccgccgtgg cgatggaatc caaacccagt
    32761 ttgtgcaaga ggcggtgcat cgtctgcaac ttggctttcg gcacgccgtg aaattggaag
    32821 gtttgccggt tggtcagccg gatggagcgg taatgactgt tttcccgggc aaatttgtcc
    32881 agttctatcc attgggacgg tttaatgatc ccgcccggca gccggcagcg caaaagcata
    32941 aatttcaagg gctcgagttt tgcctcggcg cgttcggcgc ggatgtcgcg gtcgtcctgc
    33001 tcatacatac cgtggaagcg gatgagttgg aagttgtcgc ctttgaagcc gcccgtgagc
    33061 gggtctttca aatcgtccaa aatcgtgccg cgtaaaaaat tgctttcggt tttcagacgt
    33121 tcgttgtcgg atagcggttt ttcttgccac gccaaacctt ttgtcttggt ctgtacggtc
    33181 attttgtgtt cctcccgatt atatttaatc aataaacatc acgctgatag cgtttttctt
    33241 cgcgcagcat atccaaatat tcttctgcgc cctcttcgtc caaatgtcct gccccgataa
    33301 tcacatccag caaggcggct tccacgtctt ttgccatttt tgccgcatcg ccgcacacat
    33361 agatatgcgc gccttcctgc agccattgcc aaagtccttc cgcctgttcg cggattttgt
    33421 cctgcacata gattttttct tcctgatcgc gggaccaggc gaaatcgtac ctgtgcagga
    33481 aaccgtcttt ggcaaactgc tgccattcgg tttgatagag aaaatcacgg gcaaaatgcg
    33541 gattgccgaa aatcagccag tttttgcctt ccgcattttc tgcggcacgt tgttggacga
    33601 aggcgcggaa cggtgcgacg ccggtgcccg agccgatcat cacaatcggc ttgcggctgt
    33661 cttcgggcag cctgaagccg tcgttgcgtt ccacaaacac gcgcaccgtg ccgtcctctt
    33721 ccagccggtc ggcaaggaaa cccgatgcgc cgcccgccct ggcgcgccct tcgtgttcaa
    33781 aacgcaccgc gccgacggtc aggtgcactt cgtcccccac ttccgcctgc gacgaggaaa
    33841 tcgaatacag gcgcggcgca agtgggcgca gcaggccggc gaattgttcc gccgtcagtt
    33901 ttgccgggaa gcggtgcagc acatcggcaa tcggcgtgct ttgcacaaag ccttgcaaaa
    33961 cggcgttgtc ggcagcaata cggtcgagtt cgtcatcatc ggcgaacggg gcatagcctt
    34021 tgacaaaggc gggggtgttt tgcgtgagtt caaaatggga taacagtgcg gaggcaaccg
    34081 gcagggtttt tccgcccgcc tgtatttccg ttgccggatc gatgccgagc aggtctagga
    34141 tttccctgac cagtgccgga tcgttgtcaa accaaacgcc gagcgcgtcg cccgggaggt
    34201 agtgcaaatc cgaaccgctc aaatcgattt cgatgtggcg cacgtcttta tcggattggc
    34261 gggcggtgat tttctgattg gccagcaggg cggcgggaaa ggggtctgcc ttgcagtacc
    34321 tgccctccga tgccgtctga aggccggcgg ggggcgttgc ctgcggcgcg ggcgttgccc
    34381 ggttttttgc ggcttcttct tttaagagtg cggcgatatt gtctgtccag gcgtttgcgg
    34441 cggcggcaaa gtccaaatcc gcatcaacgc gttcgagcag gcgtttcgcc cccaattctt
    34501 caaaacgttt gtcgaaatct ttgcccgccc ggcagaaatt cggataggaa ctgtcgccca
    34561 aacccagtac ggcaaattgg agtttgtcca atttcggggc ttttttgccg ttcagcagtt
    34621 tgtgcagcac gacggcttct tccggcggtt cgccttcgcc ttgggtggac gtaaccagca
    34681 gcaggcggcg ttcgccggcg atgtttttcg ccttatagtc tttcagttcg gcgcgactga
    34741 cttggatgcc ggcggcttcc aggctgtccg ccgctttgtc ggcaacggat ttcgcattgc
    34801 cggtttgcga ggcggaaagg acggttacgg aaaaaggttc tgccgtcggc aatgccgtct
    34861 gaagcgcggg cagtcctgca gatgccccgt ttcctgcttt tgcccaagcg tagccggaca
    34921 gccacgccca ttgtgccgcg tccagccccg acaggagctg tgtgatttcg ggcggcagag
    34981 gcggtaatgg cggatttgtg ttctgcatat cgtgttcact cataaaatca tacctgccgc
    35041 aacagtgccg tatgtcgctt cgtctatcag gataaacgaa ccggcggcgg tgttttccgc
    35101 ataaggcgtt gccgtaacgg gtttttgaag gttgatgcgg actttggcga tgtcgttcat
    35161 cttcaaggat tccgcgccgg cctcttgttc cagcgtgcgg acatccaaaa cgctttcaat
    35221 ttccccgact tttgccggca cggtttgcgt gccgtgcttg agcaggtatt tgcgcgcggt
    35281 gttgagcgga cgttcgtcaa accagcaaag cgtggcttcc agatgttttt gcggggcgag
    35341 cggggaattt ttatcgacaa aaaggtcgcc gcgcgaaaca tcgatgtcgc ggtccagccg
    35401 gagggttgcc gcctcgccgg caaaagcctg cgccacttcc cctttcggcg tgatgatttc
    35461 ggacacttcg gcggtcagcc cgttcggttc gatgcggacg gtttgcccga cggcgaccga
    35521 accgcgttcg atgcgcccct gatagcctcg gaaatcatcg gccttgtcgg catcttggcg
    35581 gacgaccagt tgcacgggga aataaaaatc gtcggcggtg cggctgactt cgtccgcccc
    35641 cggcagggtt tccaaaatgg acaataaggg ttcgccttta taccaaggca tattgccgcc
    35701 ggggtaaaca atgttgtcgc ccaagagtgc ggacaccggt acgaaatgca cgtctttcaa
    35761 accgagccgt ccggcgagcc ggcggtatgc ctccacaatg gcgttgaatt tgtcttcgct
    35821 gtaatccagc aggtccattt tgttgaccgc caccacaata tgcgggcagt cgagttggcg
    35881 gaggatggcg gaatggcgtt tggtctgcgg cagaagctgc aagggctgcg cgccgaaatc
    35941 cagttgggat gcgtcaacca gcacgactgc cgccgaagcg gtgcttgcgc ccgtaaccat
    36001 attgcgcgtg tattgttcgt gccccggcgt gtcggcgatg atgaatttcc gtttcgccgt
    36061 ggaaaaatag cggtatgcca catcgatcgt aatgccctgt tcgcgttcgg cttccagtcc
    36121 gtcggtcagg atggagaagt ctatggtttc tttcaaacct ttgcttttgc cggattccaa
    36181 ggttttgatt tggtcggaca gcagggcttt gctgtcgtag agcagccgtc cgatcagggt
    36241 gcttttgccg tcatcgacgc tgccggcggt aatgaagcgg agcggggcct ggtgttgtgc
    36301 cgtcatattt tcttcctcat atctgcttaa agggtttttg aaatttagaa atagccttct
    36361 tttttgcgtt tttccattgc cgcctcgctt gcctgatcgt ccagccgggt cgcgctgcgt
    36421 tcggaaatgt cggtaactgc cgtttccctg ataatctccg tcggcgtgga cgcggtgctt
    36481 tctaccgggc aggtgcagct gatatcgccg acggtgcgga agcggacatc aaggatttcg
    36541 gaggtttcag acggcatttt cggcgtaagc ggcgtaacgg ggaccagcag ccccctgcgt
    36601 ctgaccactt cgcgcctgtg gctgtaataa atcggcggca gctcgaggtt ttcgcgggcg
    36661 atgtattgcc agatgtcgag ttccgtccag ttggaaatcg ggaagacgcg catattttcg
    36721 cctttgtgca gcctggtgtt gtacagcgac cacagctcgg ggcgttgcgc cttcggatcc
    36781 cattgtccga actcgtcgcg gaacgagaaa atccgttctt tggcgcgggc tttttcttcg
    36841 tcgcgccgcg cgccgcccat cagcgcgtca aagccgtttg cctcgatggt ttccaacagc
    36901 gtaaccgcct gcgccgcatt gcgcgaatcg gtttctttgc gtaaaactac tgtgccttta
    36961 gcgatggagt cttccacgcg ccccactatc aggcgggcat tgagttttgc cgcctgcgcg
    37021 tcgcggaagg caatcacttc ggggtagttg tgccccgtgt cgatatgcac cagcgggaag
    37081 ggcagtttta ccggcctgct gcccagccgg aaggctttgc aggcgagggc gagcaggacc
    37141 acggaatctt tgccgccgga aaagagcagg gcggggtttt cgcattctgc cgccacttcg
    37201 cggataatgt ggatggattc ggattccaac cagtcgagtt ggacgttgtt cggttcggtt
    37261 ttcgtcatac gatattcctt atttcttctg tttgatcctt atgaattatt tgtgcagccc
    37321 gcattctttg ctgtttttat cttcccacca ccaccgcccg gcgcggatgt cttcgccggc
    37381 cttgacgggg cgggtgcagg ggtcgcagcc tatactggga aagccttgcc ggtacaaatc
    37441 gttgtaaggc acattgttgg caaggatgta tgcccacacg tcgtgttccg accagtcgaa
    37501 aatcgggttg tatttatcga tgccgcgccc ggcatcatat tcggcaaacg gcagttccgt
    37561 gcgtgtggcg gactgttcgc ggcgttgccc ggtaagccaa gcatccgccc cggcaatggc
    37621 gcggtcgagc ggttcggttt ttcggatgcg gcagcattcg cggcgcgctt caacgctgtc
    37681 gtaaaaggca aacctgcctt tgctttccac ataatgcagg gcatcttccc gtatcggttg
    37741 gaaacgcttg atttgcatat gcggataaac acgttcaatc ctatccaaca ggtttaaagt
    37801 ttccgcgtgc agcagccccg tatccaaagt aaaaataccg atattgaggt tttctccggc
    37861 gataaggtcg gtaattacca tatcttccgc agcaaggctg ctggcaaacc gtgcatcccg
    37921 gtggctgccg gcaatccggt gcaggcgttg tttgagggtt tccgtttttt ccgccaaagc
    37981 cgtttcactg ccattttcaa tcggcggaat tttccataaa gcaggtttga aaattttcat
    38041 tttgtccgcc ttgctttatg cggcttgttg ttcttgtccg gtgcccagtc cgtccaatgc
    38101 aaaatacgcg tgttcggccg gattgctttc ttttttggcg ttttcgccga accaagccag
    38161 tttttcgtgc agccccacca cttcgcctat gacaatcaat gccggattcg gcgcggtttc
    38221 ggcgagttcg gcaaggttgg cgagcgtgcc ggttgcggtt ttttgagccg gcagcgtgcc
    38281 ttggctgata acggctgccg gcgtgtcggg cgagcgtccg tgctgttgca gccgttcggc
    38341 aatcagggcg gctttgagcg cacccatata aatcaccaag gtctggcggc tgcgggcgag
    38401 ggtctgccat tcgatgtcgg gcgcatccgc cttgcggtgg ccggttacga aaaccgcact
    38461 ttgggcataa tcgcggtgcg tgagcgggat gccggcatag gcggtcgcgc cgacggcggc
    38521 ggtaatgccg gggacgaccg aaaacgggat ctgatggcgt gccaaggttt ccaattcttc
    38581 gccgccgcgt ccgaacacga aagggtctcc gccttttaaa cgcaccacgc gcctgccttc
    38641 gcgggccagc ctgaccataa gcgcattggt gtcctcttgc ggggtgcggc cgccccgggc
    38701 gcgcttgccg acaaaaatcc gttccgcatc gcggcggacg agggacagta tgccgtctga
    38761 aaccagcgcg tcgtaaagca ccacgtctgc ctgctggatt tcctgcagcc ctttgagcgt
    38821 cagcagcccc gcatcgccgg gacccgcacc gaccaaagac acaaaaccgc cttgataatt
    38881 ttgttccaac tgctttgcca actcctgttc ggcttgggcg gtttgttgat ttttaacgag
    38941 ggcggcaaaa cggccgttga actgcttttc ccaaaaacgg cggcgttcgg taacggattt
    39001 cagtttggct ttgaccgcat cgcgccatct tccggagatt tccgccatat cgcccaaaga
    39061 cggcggcagc agggcttcca gcctttcacg cagcagtcgg gcgaggacgg gcgcgctgcc
    39121 ggagctggaa acggcaatct gaaccgggtt gcggtcgata accgatggga agatgaagct
    39181 gcaatggtcg cggtcgtcta ccacgttgac cggcttttgg cagctttcgg caagatggaa
    39241 aacgcgccgg ttgagggctt ggtcgctgct tgccgcaatg atgaggaaaa ctgtgcgaat
    39301 gtgttcggca cgaaattctt cggcaagcca caggattttg ttttccgccg ccaacgcgga
    39361 gagttcggca ttcaggtgtt ttgcggcaac cctgacctct gcgcccgcct tcagcagcag
    39421 gctgattttg cgtgcggcga ctgcgccgcc gcctacgacc aatacggggc ggccggcgag
    39481 gttggcgaaa atggggaaat aattcactgg cgaactcctt tgctgtttgc acgcaccttg
    39541 tttccgatat ggtgcgtcgc ggtatttttg tcggaatgcg agtcatttta gacaaaagga
    39601 tttccccggt taaataataa aaaggtattt gttagaagct gaaagctata tgggggcggc
    39661 tgcggatacg gcggttttcc
//
LOCUS       DAWFJR010000009        38652 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWFJR010000009 DAWFJR010000000
VERSION     DAWFJR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38652
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.009"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..211
                     /locus_tag="WGR04_000398"
     CDS             <1..211
                     /locus_tag="WGR04_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0794024.1"
                     /translation="YTVVVPNAQSATLLPIIRKKVKPDGIVYTDTFRSYDVLDISEFS
                     HLRKFNGIPKEHLGLHLKKCQWHFK"
     gene            complement(363..1421)
                     /gene="trpD"
                     /locus_tag="WGR04_000399"
     CDS             complement(363..1421)
                     /gene="trpD"
                     /locus_tag="WGR04_000399"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ0794025.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSISQTGIGFMFAPNHHS
                     AMRYVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(1485..2075)
                     /locus_tag="WGR04_000400"
     CDS             complement(1485..2075)
                     /locus_tag="WGR04_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ0794026.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFMGLPNPVTCTRYHSLVIERGTLPECLEITAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLVEFQNFKPQKI"
     gene            2497..4773
                     /locus_tag="WGR04_000401"
     CDS             2497..4773
                     /locus_tag="WGR04_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0794027.1"
                     /translation="MAQTTLKPIVLSILLINTPLLSQAHGTEQSVGLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDNAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVYLNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSATSEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGVHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            complement(5744..6529)
                     /locus_tag="WGR04_000402"
     CDS             complement(5744..6529)
                     /locus_tag="WGR04_000402"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ0794028.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSICCGWWSLPFWVFTV
                     FALQFFRDPAREIPLNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QTESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
     gene            6684..9530
                     /gene="uvrA"
                     /locus_tag="WGR04_000403"
     CDS             6684..9530
                     /gene="uvrA"
                     /locus_tag="WGR04_000403"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ0794029.1"
                     /translation="MCKHHPQHSHDNDTIRIRGARTHNLKNVDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVCVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCENSVTGQYLSGKKSIAVPSERTPVNPGRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIDRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIASGSPEEVAKVKGSYTGKYLKNIL"
     gene            complement(9562..9732)
                     /locus_tag="WGR04_000404"
     CDS             complement(9562..9732)
                     /locus_tag="WGR04_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794030.1"
                     /translation="MFLGIFIALSGILTLLEMFNVISSDMKWGMPLMFTCLGFSIIFD
                     SFKAKQNRKQKE"
     gene            complement(9747..9944)
                     /locus_tag="WGR04_000405"
     CDS             complement(9747..9944)
                     /locus_tag="WGR04_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794031.1"
                     /translation="MEMIMTNKIKPGQNTGNNGGIYQEIGSRGGRKDNYATIKDNQKA
                     PPTQNPGSSWIQIKRTPDSSR"
     gene            complement(9948..10217)
                     /locus_tag="WGR04_000406"
     CDS             complement(9948..10217)
                     /locus_tag="WGR04_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794032.1"
                     /translation="MKIKFAHLRHPAVGGGYINFAVFDAKSTNGNHSVVLKELTTEAR
                     RNGLKIDQSALIFEQHGRTQFYGDKNLVDFLSANWYPQWTHTLTI"
     gene            10355..11296
                     /locus_tag="WGR04_000407"
     CDS             10355..11296
                     /locus_tag="WGR04_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HEZ0794033.1"
                     /translation="MEYNAIMKTIAPSHIKPLMPELNQSQRNRIIFIDFYLCFLGKIN
                     RKDLMQAFQISPAVATRDFALYCSSFSKNLRFDTKSKFYLSTEDFEPQFPHSFENAIK
                     ILNGSLIFDIDSQHQPLLPFDLPPVLINPDVLIFSKISKALSLKKPLKISYHSISSGL
                     SEKVIVPLALVNDGFRWHLRAFDRTKNRFADFVLTRIIQANILLAENIEENETINNDI
                     DWNRIVKIELVPHPNHDEAKKVIEMDFGMTNGKLELALRASNIGYVLQQWRVDCSSDH
                     HIDDPAFRLWLLDNLVLYGINNAEHLAPAFDKKRSKP"
     gene            complement(11523..11789)
                     /locus_tag="WGR04_000408"
     CDS             complement(11523..11789)
                     /locus_tag="WGR04_000408"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794034.1"
                     /translation="MKRQTVNQATACVRTAHTLHVDFEVPCNSQVGCVAQPRTRCWVC
                     RLRLAAAETPEEKAKDAAEEAKEEAKDAAEDAKEAAEDAKEEAK"
     gene            12038..12367
                     /locus_tag="WGR04_000409"
     CDS             12038..12367
                     /locus_tag="WGR04_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0794035.1"
                     /translation="MDVQKQYSFETVAVCVLPNHIHAIWTLPPDDADYSLRWRLIKTK
                     FSAHFPHAENLSASKQRRHERGIWQRCFYEHTVRDETDLQHRADYIHSNPIKQRMSRA
                     KTESRKK"
     gene            complement(12604..13494)
                     /gene="sucD"
                     /locus_tag="WGR04_000410"
     CDS             complement(12604..13494)
                     /gene="sucD"
                     /locus_tag="WGR04_000410"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ0794036.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKTKGLA"
     gene            complement(13505..14671)
                     /gene="sucC"
                     /locus_tag="WGR04_000411"
     CDS             complement(13505..14671)
                     /gene="sucC"
                     /locus_tag="WGR04_000411"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ0794037.1"
                     /translation="MNLHEYQAKELLASYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRITFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVQGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            complement(14770..15057)
                     /locus_tag="WGR04_000412"
     CDS             complement(14770..15057)
                     /locus_tag="WGR04_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794038.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            complement(15182..16615)
                     /gene="lpdA"
                     /locus_tag="WGR04_000413"
     CDS             complement(15182..16615)
                     /gene="lpdA"
                     /locus_tag="WGR04_000413"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0794039.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            16812..16928
                     /locus_tag="WGR04_000414"
                     /pseudo
     CDS             16812..16928
                     /locus_tag="WGR04_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(16980..18173)
                     /gene="odhB"
                     /locus_tag="WGR04_000415"
     CDS             complement(16980..18173)
                     /gene="odhB"
                     /locus_tag="WGR04_000415"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ0794040.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPVAAPAEAAPAAAPAA
                     APDAAQNNAAMPAATKLAAETGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAVA
                     LPAGARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEK
                     EHGVKLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPIL
                     RDADQMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQ
                     SAILGMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPAR
                     LLLDL"
     gene            complement(18273..21101)
                     /locus_tag="WGR04_000416"
     CDS             complement(18273..21101)
                     /locus_tag="WGR04_000416"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ0794041.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(21306..22589)
                     /gene="gltA"
                     /locus_tag="WGR04_000417"
     CDS             complement(21306..22589)
                     /gene="gltA"
                     /locus_tag="WGR04_000417"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0794042.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(22701..22949)
                     /locus_tag="WGR04_000418"
     CDS             complement(22701..22949)
                     /locus_tag="WGR04_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ0794043.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(22953..23660)
                     /locus_tag="WGR04_000419"
     CDS             complement(22953..23660)
                     /locus_tag="WGR04_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ0794044.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(23780..25543)
                     /gene="sdhA"
                     /locus_tag="WGR04_000420"
     CDS             complement(23780..25543)
                     /gene="sdhA"
                     /locus_tag="WGR04_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ0794045.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDINTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(25546..25887)
                     /gene="sdhD"
                     /locus_tag="WGR04_000421"
     CDS             complement(25546..25887)
                     /gene="sdhD"
                     /locus_tag="WGR04_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ0794046.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(25881..26258)
                     /gene="sdhC"
                     /locus_tag="WGR04_000422"
     CDS             complement(25881..26258)
                     /gene="sdhC"
                     /locus_tag="WGR04_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ0794047.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(26537..27940)
                     /locus_tag="WGR04_000423"
     CDS             complement(26537..27940)
                     /locus_tag="WGR04_000423"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0794048.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(28189..28926)
                     /locus_tag="WGR04_000424"
     CDS             complement(28189..28926)
                     /locus_tag="WGR04_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ0794049.1"
                     /translation="MALQDRAGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAICCVSVNDTFVMNAWSAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(29205..31481)
                     /gene="metE"
                     /locus_tag="WGR04_000425"
     CDS             complement(29205..31481)
                     /gene="metE"
                     /locus_tag="WGR04_000425"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ0794050.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQVAANADFVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLNKVSAKILLSTYFGSVAEHAALLKALPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGVIDGRNIWRANLNKVLKTVESLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAAGSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSVADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTHKLRAELAK"
     gene            complement(31619..32497)
                     /gene="metF"
                     /locus_tag="WGR04_000426"
     CDS             complement(31619..32497)
                     /gene="metF"
                     /locus_tag="WGR04_000426"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ0794051.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            32697..32972
                     /locus_tag="WGR04_000427"
     CDS             32697..32972
                     /locus_tag="WGR04_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ0794052.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            32972..33097
                     /gene="ykgO"
                     /locus_tag="WGR04_000428"
     CDS             32972..33097
                     /gene="ykgO"
                     /locus_tag="WGR04_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ0794053.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
     gene            complement(33203..33403)
                     /locus_tag="WGR04_000429"
     CDS             complement(33203..33403)
                     /locus_tag="WGR04_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794054.1"
                     /translation="MIGRLFRIFFFFALAALIMNRLFSRRQKRALREVAEISARVLLG
                     AAAATLFWYLFMLYFKHIPDSY"
     gene            33546..34685
                     /locus_tag="WGR04_000430"
     CDS             33546..34685
                     /locus_tag="WGR04_000430"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ0794055.1"
                     /translation="MSQNASVGIVMPQKIPFEVPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPLVTVKDWVKSQAALADYLSIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSRNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGDSLTRALQNVKAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKGCQQ"
     gene            34682..35263
                     /gene="metW"
                     /locus_tag="WGR04_000431"
     CDS             34682..35263
                     /gene="metW"
                     /locus_tag="WGR04_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ0794056.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            complement(35285..35392)
                     /locus_tag="WGR04_000432"
                     /pseudo
     CDS             complement(35285..35392)
                     /locus_tag="WGR04_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            35486..36322
                     /locus_tag="WGR04_000433"
     CDS             35486..36322
                     /locus_tag="WGR04_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ0794057.1"
                     /translation="MKNKTSSLLLWLAAIMLAACSPSKEDKTKETGASAASSSASSAP
                     SQTDLQPAASAPDNVKQAESVPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALSHHE
                     LETRFGLPDSGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDALME
                     QERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYIGQL
                     PGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNPNLG
                     RR"
     gene            complement(36372..36932)
                     /gene="efp"
                     /locus_tag="WGR04_000434"
     CDS             complement(36372..36932)
                     /gene="efp"
                     /locus_tag="WGR04_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ0794058.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(36975..38123)
                     /gene="earP"
                     /locus_tag="WGR04_000435"
     CDS             complement(36975..38123)
                     /gene="earP"
                     /locus_tag="WGR04_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ0794059.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSLRLARVLHRELGWRVHLWTD
                     DVSALRALCPDLPDVPCVHQDIDVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSGALRKRLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYSPETASVHRRLSDDLNGGEALSAT
                     QRLECWQTLQQHQNGWRQGAEDWSRYLFEQPSAPEKLAAFVSKHQKIR"
     gene            complement(38142..38420)
                     /locus_tag="WGR04_000436"
     CDS             complement(38142..38420)
                     /locus_tag="WGR04_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794060.1"
                     /translation="MSRNLLVRWLAVCLIPLATLAVFAANPPEDKLQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMFLFIPIVLLVVYLFHYFGAF"
BASE COUNT         9661 a        10021 c         9346 g         9624 t
ORIGIN      
        1 ttataccgtc gttgttccta atgcgcaatc agcgacattg ttgccaatta tccgaaagaa
       61 agttaagcct gatggcattg tatacacgga tacctttcgt agttatgatg tgcttgatat
      121 tagtgaattt agccatttac gtaagtttaa cggcattccc aaagagcatt tggggctgca
      181 tttaaagaaa tgccaatggc attttaaata gaatctcaaa ttcagatttt aagacaatta
      241 gttaatggga atttggtctg gttatctagt acagccccaa gtttttatat aaaaacagtt
      301 tggtaagttc tcttcgtgaa gtgcttatgt tttgccacaa aagttccata atcatggcgg
      361 catcacccaa gctccatctt tccaagaaaa tggcatcttt gttgtggttg aaaaccgaca
      421 aactcctctt ttttcgattt ggccctgcct gaatcgatgg cttcccgtgc ggcagatatg
      481 ccgtctgaaa gcgaagcagc gacatttccg gcatacaggg cggcggcggt gttgagcaat
      541 acgatatcgc gcgcagcccc ttctcttcct tccagcacct cattcatttt caacaaagat
      601 tcctgagtat tggcaacttt gatttcatcc aaattgcggc gggtttcgat accgaaatct
      661 tctgggcgga tgtcgtattc gctgattttt ccgtctttga gctcggcaac gcgtgttttg
      721 cccgtcagtg taatttcatc caaaccgccc tccccgcaaa caaccaaaac atgcttggag
      781 ccgagctgtt gcaatactct ggaaaggatg ccgcacaaat cggtgtggaa cacgcccaaa
      841 agctggttcg gcgcgcccgc aggattcgtt aacggaccca atatgttgaa aatacttcgg
      901 aaaccgagcg aacggcgcac cggggcgaca tagcgcatag cgctgtggtg attgggtgcg
      961 aacataaacc ctataccggt ctggctgata ctttgggcaa tctgttcggg agtcaggttg
     1021 aggtttgcgc ccatttgctc catcacgtca gccgcaccgc tggaggaaga gaccgaccgg
     1081 cctccgtgtt tggcaacctt cgcgcctgcc gctgcggcaa caaacatcga agtcgtcgaa
     1141 atattgaagg ttttggcacc atccccgccc gtaccgacga tatcgaccag cccctctgca
     1201 ttctccagcg gcacttttgt cgcaaactcg cgcatgacgg cggcggcggc ggtaatttcg
     1261 gaaacggttt caaccttgat acgcaatcct gtcaaaatcg ccgcaatttg ttcgggcggc
     1321 acttttccgc tcatcatttg gcgcatcaag tcggtcattt catcgtaaaa caactcatta
     1381 ttgctgatta atcgttcgat agcctgttgc ggtgtaatca ttttttgtcc tccgttcaat
     1441 attcggacga aaatgccgtc tgaagggctt cagacggcat cacgtcagat tttttgcggt
     1501 ttgaagtttt gaaattcaac taaaaaattg tttaacatat catgtccgtg ttcggtcagc
     1561 agagcttcag ggtggaattg taccccctcg acggcatatt ccttatggcg cacgcccata
     1621 atctcgccgt cttccgtcca tgccgtgatt tccaaacatt cgggcagtgt gccgcgttca
     1681 ataacgaggc tgtgataacg cgtacaggta accggattgg gcaaccccat aaacataccc
     1741 ttgcccgaat gggacacggg cgacaccttg ccgtgcatca gcgttttggc gcggactatc
     1801 ctgccgccga acgcctcgcc tatcgtctga tgcccgagac acacgcccat aatcggcagc
     1861 cggccggcaa aatggcgcat cgccgccacg gaaatccccg cttctttggg cgaacacgga
     1921 ccggggccga taacgagata ttgcggattc aatgcctcga tttcttccaa tgtaatatca
     1981 tcgttgcggc gcacggcgac ttcctgcccc aattcagcga aatactggac gatgttgtaa
     2041 gtaaaactgt cgtaattgtc gataaacaaa agcattttgt gattaaccta ttgatttata
     2101 atttgtttgt tattaactgc atttccgata cccccatcca taccctctta tatcttagcg
     2161 tgcccgatgc accctcgtga acctggcgca gagcctcgcg ttctgacaag tacgccattt
     2221 tgccaccttt tcgaccgttc gggaacgtac cgtttttatg cactcataaa ttttggttat
     2281 cccgcagccg aacatagtgg gcaaacacag cggaatctgc gtcaacatca accgcgcccc
     2341 cgaatgacgg cggatacaaa tgaacaccgc ccgatgccgc aaccgtgtca tttgaaaaac
     2401 ttttgcctat gccctctgag tgttctcaaa atcttatgat ataaaaaatg taatgttata
     2461 taataacaaa cttttgaaat gcgacgagaa gccaaaatgg cacaaactac actcaaaccc
     2521 attgttttat caattctttt aatcaacaca cccctcctct cccaagcgca tggaactgag
     2581 caatcagtgg gcttggaaac ggtcagcgtc gtcggcaaaa gccgtccgcg cgccacatcc
     2641 gggctgctgc acacttcgac cgcctccgac aaaatcatca gcggcgacac cttgcgacaa
     2701 aaagccgtca acttgggcga tgctttggac ggcgtgccgg gcattcacgc ctcgcaatac
     2761 ggcggcggcg cgtccgctcc cgttattcgc ggtcaaacag gcaggcggat taaagtgttg
     2821 aaccatcacg gcgaaacagg cgatatggcg gatttttcgc ccgatcacgc cattatggta
     2881 gataccgcct tgtcgcaaca ggtcgaaatc ctgcgcgggc cggttacgct cttgtacagc
     2941 tcgggcaatg tggcggggct ggtcgatgtt gccgatggca aaatccccga aaaaatgcct
     3001 gaaaacggcg tatcgggcga actcggattg cgtttgagca gcggcaatct ggaaaaactc
     3061 acgtccggcg gcatcaatat cggtttgggc aaaaactttg tattgcacac ggaagggctg
     3121 taccgcaaat cgggggatta cgccgtaccg cgttaccgca atctgaaacg cctgcccgac
     3181 agccacgccg attcgcaaac gggcagcatc gggctgtctt gggttggcga aaaaggtttt
     3241 atcggcgtag cgtacagcga ccgtcgcgac caatatggtc tgcctgccca cagccacgaa
     3301 tacgatgatt gccacgccga catcatctgg caaaagagct tgattaacaa acgctattta
     3361 cagctttatc cgcacctgtt gaccgaagaa gacatcgatt acgacaatcc gggcttgagc
     3421 tgcggcttcc acgacgacga taatgcacac gcacacaccc acagcggcag accgtggata
     3481 gacctgcgca acaaacgcta cgaactccgt gccgaatgga agcaaccgtt ccccggtttt
     3541 gaagccctgc gcgtatactt gaaccgcaac gactaccgcc acgacgaaaa agcaggcgat
     3601 gcggtcgaaa acttttttaa caaccaaacg caaaacgccc gcatcgagtt gcgccaccaa
     3661 cccataggcc gtctgaaagg cagctggggc gtgcaatatt tacaacaaaa atccagtgct
     3721 ttatctgcca catccgaagc ggtcaaacaa ccgatgctgc ttgacaataa agtgcaacat
     3781 tacagctttt tcggtgtaga acaggcaaac tgggacaact tcacgcttga aggcggcgta
     3841 cgcgtggaaa aacaaaaagc ctccatccgc tacgataaag cattgattga tcgggaaaac
     3901 tactacaacc atcccctgcc cgacctcggc gcgcaccgcc aaaccgcccg ctcattcgca
     3961 ctttcgggca actggtattt cacgccacaa cacaaactca gcctgaccgc ctcccatcag
     4021 gaacgcctgc cgtcaacgca agagctgtac gcacacggca aacacgtcgc caccaacacc
     4081 tttgaagtcg gcaacaaaca cctcaacaaa gagcgttcca acaatatcga actcgcgctg
     4141 ggctacgaag gcgaccgctg gcaatacaat ctggcactct accgcaaccg cttcggcaac
     4201 tacatttacg cccaaacctt aaacgacgga cgcggcccca aatccatcga agacgacagc
     4261 gaaatgaagc tcgtgcgcta caaccaatcc ggtgcggact tctacggcgc agaaggcgaa
     4321 atctacttca aaccgacacc gcgctaccgc atcggcgttt ccggcgacta tgtacgaggc
     4381 cgtctgaaaa acctgccttc cctacccggc agggaagatg cctacggcaa ccgtcctttc
     4441 atcgcgcagg acgaccaaaa cgcccctcgc gttccggctg cgcgcctcgg cgtccacctg
     4501 aaagcctcgc tgaccgaccg catcgatgcc aatttggact actaccgcgt gttcgcccaa
     4561 aacaaactcg cccgctacga aacgcgcacg cccggacacc atatgctcaa cctcggcgca
     4621 aactaccgcc gcaatacgcg ctatggcgag tggaattggt acgtcaaagc cgacaacctg
     4681 ctcaaccaat ccgtttacgc ccacagcagc ttcctctctg atacgccgca aatgggccgc
     4741 agctttaccg gcggcgtgaa cgtgaagttt taaaatcgga caggcaaaca aaaaaggttt
     4801 tcagacggca ttctgaaaac cttttttctt tttaatcaat ttgttaggaa atatggcaaa
     4861 gacaaagaac aaaccactat gccatcattc ccattttctt tgtttcatca ttcttgcttt
     4921 cacgccgtca ttctcgcaaa agcagaaaaa tcaaaagcag aaacccatca ttcccgcgca
     4981 ggcgagaatc caattcattc ggtttcagcc gtttccgata aatcactgta acgttaagtt
     5041 cccggattcc cactttcgtg ggaatgacgg cgtaaaggtt tctgtttttt aagttttagg
     5101 taactaatga gtcgtcattc ccgcgcaggc gggaatccaa ttcgttcggt ttcagtcgtt
     5161 tccgataaat cgccttagca tttgggttct agattcccac tttcgtggga atgacggcgt
     5221 aaaggtttct gttttttaag tgttaggtaa ctaataagtc gtcattcccg cgcaggcggg
     5281 aatccaattc gttcggtttc tgttatttcc gataaatcac tgtaacgtta agttcccgga
     5341 ttcccacttt cgtgggaatg acggcgtaaa ggtttctgtt ttttaagttt taagtaacta
     5401 atgagttgtc gttcccacgc aggcgggaat ccggtctctt cggtttcagt cgtttccgat
     5461 aaatcgtcgt aacgttaagt tcccggattc ccactttcgt gggaatgacg cggaaaggtt
     5521 tctgtttttt aagtgttagg taactaataa gtcgtcattc ccgcgcaggc gggaatccag
     5581 tccgtccggt ttcagtcgtt tccgataaat cgtcgtaacg ttaagtttct agattcccac
     5641 tttcgtggga atgacggtgt gaacattagc aaaaattcaa aaaaccaaac ttcctcattc
     5701 gtgtgaatga tggtactgat attttctatt tcagtccgca aaattaatct ttcaacacat
     5761 cttgcacagc cgcctgaatc ttagccgccg ctgcctcgat ttgccgttgt tcggcagatg
     5821 ggtttgccgc tgtttcaacc ggagcagttt gtaaagcagg ctcagattca gtttgcggcg
     5881 cagtcagcgg caaacgcgcc aatacagtgc tgacgccggt tactttatcg ccaatcgcca
     5941 cttgcgcctg cgcatcgaca ggcagataca tatccacgcg cgaaccgaag cggataaagc
     6001 cataacgttc gccgcgggac agtttcgcac ctgcttgggt gtagcacaaa atacggcgcg
     6061 ccaccaaacc ggccacttga acaaaagtaa tttcacgacc tgaagccgta gtcgccaaca
     6121 ccgcattacg ttcgttttcc gtgctggctt tgtccaaatc cgcattcacg aatttgcctt
     6181 tgttatagac cactttcgtt accgtacaat cggcaggcga tttttgcgaa tgcacgttga
     6241 acacgttcat aaaaatactg attttcaaag catcgacatc acgatacgga tcgcgtgcgc
     6301 gttcgaccac cacgatacgg ccgtcaaccg ggctcaacac cgcttcagga tttagcggaa
     6361 tctcacgcgc agggtcgcgg aaaaactgca atgcaaatac ggtaaacacc caaaacggca
     6421 aagaccacca gccgcaacat atcgacacca gcaggctcaa agccaaaccg ccgccaataa
     6481 tcggccagcc ctcacgggcg ataatcgggt gggggtaaag acggttcatt tgtcatcctt
     6541 ttgttcaagc gggttaaaat tgccgacatt atagcggaat ctgccgtact ccgctggcaa
     6601 atgccgtctg aacgcggctt cagacggcat ccgagaaagt gctacaatac cagttttcct
     6661 ttatttttca ggcggcatcc attatgtgca agcatcatcc ccaacattcg cacgacaacg
     6721 acaccatccg catccgcggc gcgcgcacgc ataatttgaa aaacgtcgat ttggacattc
     6781 cgcgccacaa gctcgtggtg gtaacaggat tgtcgggcag cggcaagtcg tcgctggcgt
     6841 ttgacacgct gtatgccgaa gggcaacggc gttatgtcga gagcctttcc gcctatgcgc
     6901 ggcagttttt gcagatgatg gacaaacccg atgtcgattt gatcgaaggc ctctcgcctg
     6961 cgatttccat cgagcagaaa tccaccagcc acaacccgcg ttccaccgtc ggcacggtta
     7021 cggaaatcca cgactacctg cgccttttgt acgcccgcgt cggcacaccg tattgccccg
     7081 aacacaatct gccgctgtcg agccaaaccg tatctcagat ggtcgatgcc gtattaaaac
     7141 tgccggaaga tacgcgcgtg atgattcttg cgccggcagt tcgtgagcgt aagggcgagt
     7201 ttgtcgactt ctttgccgac ttgcaggcgc aaggctttgc ccgtgtgtgc gtggacggcg
     7261 aagtttatca gcttgacgaa gtgccgaagc tggaaaaaaa catcaaacac aatatcgacg
     7321 tggtcatcga ccgcgtgaaa gtgaaggcgg acatcaagca gcggctggcg gaaagttttg
     7381 aaaccgcgct gcgccacggc aacgagcgcg cgctggcgat ggaaatggac agcggcgaag
     7441 aacattggtt ttccgcgcgt tttgcctgcc ccgtgtgttc gtacagcctg cccgaattgg
     7501 agccgcgcct cttttcgttc aataatccga tgggctcctg cccgacttgc gacggcttgg
     7561 gcaacaccaa tttcttcgac cccgaaaaag tagtcgccca tcccgaattg tcgctggcaa
     7621 cgggcgcgat tgacggctgg gacaagcgca accagttcta tttccaaatg attcagtcgc
     7681 tggcgcggca ttacggtttt gatgtgcagg ctgcttggga aacgctacct gaaaaagtga
     7741 aaaaagtcgt gctgcacgga tcaggcaagg aagtcattga tttcacctac ctatccgaac
     7801 gcggcaccac cttcaaccgc agccacgcct tcgaaggcat cattcccaat ctcgaacgcc
     7861 gctaccgcga aaccgacagc gaaaccgtgc gcgaaaaatt gcgcgaatac caaaaccacc
     7921 gcgcctgccc gagctgcggc ggtgcacgtt tgcgcaaaga agcgcgctac gtttacgtca
     7981 gcggcgaacc tttacacgaa gtctccgcct ggccgctgac caaaacccac cgattctttg
     8041 aaacgctgga tttggacggc aacaaaaaac aaatcgccga aaaaatcctc aaagaaatca
     8101 ccgagcggct cggctttttg attaacgtcg ggctggatta cctgaatctc tcccgctccg
     8161 ccgaaaccct ttccggcggc gaagcccagc gcatccgcct tgccagccaa atcggcagcg
     8221 gcctgaccgg cgtgatgtac gttttggacg aaccctccat cggcctgcac cagcgcgaca
     8281 acgaccgcct gctcgccacc ctcaaacgcc tgcgcgattt aggcaacagc gtgattgtgg
     8341 tcgaacacga cgaagacgcc atccgcgaag ccgatttcgt cgtcgatatg ggcccgggtg
     8401 cgggtgagca cggcggcaac gtactgattg ccgacacgcc cgaaaacgtc gccaaatgcg
     8461 aaaactccgt taccggacaa tacctcagcg gcaaaaaatc cattgccgtg ccgtctgaac
     8521 gcacgcccgt caatcccggc cgaatgctcg tcctcaaagg cgcgcgcggc aacaacctca
     8581 aaaacgttac ccttgagctg ccgctcggtt tgattacctg catcaccggc gtatccggca
     8641 gcggcaaatc caccctgatt aacgacaccc tcgccaaaat cactgcccgc gaactcaacc
     8701 gcgcccaaga agaacccgcc ccatacgacg acatccgcgg cctcgaacac ctcgacaaag
     8761 tcatcaacgt cgaccaatcc cccatcggtc gcaccccgcg ctccaacccc gccacctaca
     8821 ccggcctgtt cacccccatc cgcgaactct tcgccggcgt acccctctcg cgcgaacgcg
     8881 gctacaacgt cggcagattc tccttcaacg tcaaaggcgg ccgctgcgaa gcctgccaag
     8941 gcgacggcgt gattaaagtc gaaatgcact tcctgcccga cgtgtacgtc ccctgcgaag
     9001 tctgccacgg caaacgctac aaccgcgaaa ccctcgaaat ccaatacaaa ggcaaaaaca
     9061 tcagtcaagt cctcgacatg accgtcgaag aagcccgcga attcttcgac gccgtcccca
     9121 ccgtatcgcg caaactgcaa accttgatgg acgtaggctt aggctacatc cgtctcggcc
     9181 aatccgccac caccctctcc ggcggcgaag cccagcgcgt caaactcgcc ttggaactct
     9241 ccaaacgcga caccggcaga acgctctaca tcctcgacga acccaccacc ggcctacact
     9301 tcgccgacat cgccctgctg ctggaagtca tagaccgtct gaaaggcaaa ggcaactcga
     9361 tagtgattat cgagcataat ctggatgtga ttaaaaccgc cgattggatt gtggacttag
     9421 ggccagaagg cggcgacggc ggagggagga ttattgcttc aggtagcccg gaggaagtgg
     9481 cgaaggttaa ggggagttat actgggaagt atttgaagaa tatactttaa aaaacgggat
     9541 ggtttaaacc atcccgtttt tttattcctt ctgttttcga ttttgtttgg ctttgaagct
     9601 atcaaaaata atgctaaatc ccaaacaggt gaacattaaa ggcatccccc atttcatatc
     9661 cgaagaaata acattaaaca tttctaataa tgtaagaatg cctgataaag ctataaaaat
     9721 acctaaaaac attagaacta tccttattat ctggagctat ctggagttct tttaatttga
     9781 atccatgagc ttcctggatt ttgagtgggt ggtgcttttt gattatcttt aatggttgcg
     9841 tagttatctt ttcgaccacc tcgtgaacca atttcttgat aaattccacc gttatttcct
     9901 gtattttgac caggtttgat tttgttcgtc attatcatct ccataattta aatagttaaa
     9961 gtatgagtcc attgcggata ccaatttgca gataaaaaat ctactagatt tttgtctcca
    10021 tagaattgtg tacgcccatg ttgttcaaat attaacgcag attggtcaat ttttaatcca
    10081 tttctacgag cttcagtggt caattccttc agtacgacac tatggttacc attagttgat
    10141 ttagcatcaa aaacagcaaa gttaatatat cctccaccta cggctggatg tcttaagtga
    10201 gcaaatttta ttttcataaa taaatcctat aaaaaattac ataacagaaa ttttaagtca
    10261 gagagctact atgaagtagc tttttgcatt atatgcactc tttttgatta cgtcaagcaa
    10321 tttttttgaa ttcatattgc catgaaaaag attgatggaa tataatgcaa ttatgaaaac
    10381 aatcgctcct tcccatataa agccccttat gccagagttg aaccaatctc aacgcaatag
    10441 gattatattt attgatttct atctatgctt tctaggaaag ataaatagaa aagatttgat
    10501 gcaggcattt caaatttccc cagctgttgc tactagggat tttgcgctat attgctctag
    10561 tttttcgaaa aatctccgtt ttgataccaa aagtaaattt tacctctcta ctgaagactt
    10621 tgaacctcaa ttcccccatt catttgaaaa tgcaattaaa attcttaatg gcagtttaat
    10681 atttgatatt gattcccaac atcaacctct tcttccgttt gatttacctc ctgttttaat
    10741 aaatcctgat gtcttaatct tttctaaaat atcgaaagca ttatccttaa aaaaacctct
    10801 aaaaatctca tatcattcaa tttcatctgg attatctgag aaagttattg tgcctcttgc
    10861 tctagttaat gacggatttc gatggcattt acgggcattc gatagaacaa aaaaccgatt
    10921 tgcagatttt gtcctaacac gaataattca agcaaatatt ttattagctg aaaatattga
    10981 agaaaatgaa acgattaaca atgatattga ttggaataga attgttaaaa tcgagttggt
    11041 tccccatcct aatcatgatg aagctaaaaa agtgattgaa atggactttg gaatgacaaa
    11101 tggaaaatta gaattggcgc ttagagcttc aaatatcgga tatgttttac agcaatggcg
    11161 agtcgattgt tcgtcagatc atcatatcga tgatcctgct tttagattat ggctgttaga
    11221 taatttagtt ttatacggca tcaacaatgc tgaacactta gcgcctgcct ttgataaaaa
    11281 acgtagcaag ccgtagccta catatccaac cgaccgcgtg cgtggctgcg ccacacacgc
    11341 ggttgtttga ttgatggctt tctgttgtaa attgaactta gttaaaacta acactttcag
    11401 acggcatgaa aaatggccgt ctgaaaactg aaaaagcttt atctaaaaca actaccctaa
    11461 ctcacaaaac aaaaaacgga ttcccaatca ttggaatccg tttttctgta aagctatccg
    11521 aattatttag cttcttcttt agcgtcttca gcagcttctt tggcatcttc cgctgcgtct
    11581 tttgcttctt ctttagcctc ttcagcggcg tctttggctt tttcttcagg agtttctgca
    11641 gcagcaagcc gtagcctgca tacccaacac cgcgtgcgtg gctgcgccac acaccctacc
    11701 tgtgaattac acggaacttc aaaatcaacg tgtagggtgt gtgcggtacg cacgcacgcg
    11761 gttgcttgat tgacggtttg ccgtttcaac ttttaacttc gttgaagcta gcgctttcag
    11821 acggcctcca atgtcgtctg aaagcgctag cttcaacgaa gttaaaaacc ttaaacaaca
    11881 agccattcct tctccaacgc tcaaaccaaa aacaaaaatc ctatggcgcg ttatcgcaga
    11941 aacttcattg ccggcggcac attctttttc accgtcaaac tcgccgaccc gaaatcgcgc
    12001 ctgcttgtcg aacatatcga cttcgcgggc ggcttatatg gatgtgcaaa aacaatattc
    12061 ctttgaaacc gtcgccgtat gcgtgctgcc gaaccatatc cacgccattt ggacgctgcc
    12121 acccgacgat gcggattatt ccctgcgctg gcggctgatt aaaaccaaat tctccgcaca
    12181 tttccctcat gccgaaaacc tgtctgccag caaacagcgg cggcacgaac gcggtatttg
    12241 gcaacggtgt ttttacgaac acaccgtgcg cgatgaaacg gatttgcagc atcgtgcgga
    12301 ctacatccat tccaatccta tcaaacaaag aatgtcgcgt gctaaaacag aatcccgcaa
    12361 aaaataatat tagtcttttc aaatgcgata cttatccatt cccggacttc aaacaaggcg
    12421 tgtgcaacct gacttacggg tttgctcctg tttgaccgat aaatattctg actgcctcaa
    12481 agggcaaaaa ataatgtggg tatgttgagg ctgcctcttt ttgaaatgac aaacaaaatg
    12541 ccgtctgaaa cctgatttcc ggtttcagac ggcatttgat gttcaatcag ttgtcaaacc
    12601 tgattatgcc aaaccttttg ttttcaacac ttccagcatg gtagtgccca actcggcagg
    12661 gctgcgggtg taagcgatac cggctttttc gaaagcggcg aatttttcct cggcagtacc
    12721 tttaccgccg gaaataatcg caccggcgtg gcccatacgt ttgcctttag gagcggtaac
    12781 accagcaatg taacctacaa caggtttggt tacgttggat tggatgtatt cggctgcttc
    12841 ttcttccgca gtaccgccga tttcaccgat catgatgatg gcgtcggtat ctgggtcttc
    12901 ttggaaaagt ttcagtgcgt cgatttggtt cataccggga atcgggtcgc cgccgatacc
    12961 gatacaggtt gattgaccca agcccagttt ggtggtttgt gccacggctt cgtaagtcaa
    13021 tgtaccggaa cgggaaataa taccgatgcg gcccggagtg tggatgtggc ccggcataat
    13081 accgattttg cactcgcctg gggtaatcac gcccgggcag ttagggccga ccaagcgggt
    13141 accgttgccg ttggtttcca agtaacgttt ggctttgagc atgtccagag tcggcacgcc
    13201 ttcggtaatc acaaccacca agcctacgcc ggaatcaacg gcttcaacga tagaatccaa
    13261 cacaaacgga gcgggaacgt aaattacaga tgcatccgcg ccggtttctt taacggcttc
    13321 tttcatggtg ttgaacacgg gcaggttcag gtgggtttga ccgcctttgc ccggggtcac
    13381 gccgccgaca actttagtgc cgtaagccag agcttgttcg gagtggaaag taccgttttt
    13441 accggtgaaa ccttgaacca atactttggt gtctttatta atcaatacgc tcattctttt
    13501 ctccttaggc gtttacggct gcgacaattt tttcggctgc gtcattcagg ccgtctgcag
    13561 aagtcagttt cagacctgat tcgttcagga ttttcgcgcc gagttcggcg ttgttgcctt
    13621 ccaaacgaac aacgacagga acgtcgacgt tgatttcttt aacggctgcc acgatggctt
    13681 ccgcaatcat gtcgcaacgt acgataccgc cgaagatgtt gatcaatacg ccttgaacgg
    13741 atttgtcttc cagaatcagt ttgaacgctt caaccacgcg gtctttggtt gcgccgccgc
    13801 caacgtccaa gaagttggca ggttggccgc ctttcagttt gatgatgtcc atagtggcca
    13861 tcgccaaacc ggcaccgttc accatacagc cgatgttgcc ttccagggca acatagttca
    13921 ggtcaaattc agaagctttc aactcgcgtt cgttttcttg agatttgtcg cgcaattcgg
    13981 cgattttcgg caggcggtag agcgcgttgc tgtcgatgcc gattttgccg tccacgcagg
    14041 cgagcgcgcc gttttcgcga actgccagcg ggtttacttc aaacagggcg aagtcatttt
    14101 cgacaaacgc tttgtacgca ccggtcatca gtttgacgaa ctcgttgatt tgtttgtctt
    14161 tcaagcccag ttggaacgca acttcgcgag cttggcaagg ttgcaggccg accagcggat
    14221 caacggttac tttgaagatt ttttcaggag tttcggcagc aactttttcg atttccacgc
    14281 cgccttcggt agaggccatg aatgtaatgc ggcgggtaga acggtcaacc actgcgccca
    14341 agtacagctc ggtttgaacc ggatacatgt cttcgcaaac caaaacactg ttgacaggtt
    14401 ggccgttggc atcggtttgg taagttacca agttggtgcc aatcaggctt tcagccactt
    14461 ctttagcttc ttcgcggctt ttaacgactt ttacgccgcc cgctttaccg cggccgccgg
    14521 cgtgtacttg tgctttgaca acagcgaatt tgccgcccaa tttgtcgtaa gctgcagcgg
    14581 cttcttcgcc gttgtgtgcc aaaataccgc cttgtacggg caaaccgtag ctagccagca
    14641 gttctttagc ctgatactcg tgtaaattca tggatttctc ctttagatta gtggaatgcc
    14701 ccgagtctgc ctccaaatgg caagcccgtg cgtttcagac tacctttcgg cggtcggaaa
    14761 atcgtcgttt cacttgctga aaggcaacca catccgcgcc agcagataca tggctgcacc
    14821 gcctcccccg agaataaaaa agacgatgcc ttttttacgc gaattcggac agagcagata
    14881 aacggcaagg ctgaaactga taaacaacaa tgccacgcgc caaaccaatt ctttatcttt
    14941 gaaacggttg agcgtataag tttcggtcat catcacatac atctgccaca atgcggccat
    15001 acacatgctg atgatgccca tcggtataaa gaaaataccg actatttctt tgttcataac
    15061 gaatgtaaat gctgtttgtt tgtctgacgg aattataaat cagaaaacgg agctctcgga
    15121 acattcaatt tgtcgaaggc attaaggtcg tctgaaaaat ttcagacgac cttaatgtct
    15181 atcaaccgtg caaagcgcgt ttgtcggccg ccaatgcagc ttcgtgaacc acttcggaca
    15241 aggttgggtg ggcatggata atgcgggcga tgtcttcgct gctggcgaag aattcgagcg
    15301 cagtcacgcc ttcggtaacc aattcgctga caaccggacc aatcatgtgt acgcccaaga
    15361 tgcggtcggt tttggcgtct gccaacactt taaccgtacc tttggctttg cccattgcca
    15421 atgcgcgacc attcgcacca aaacctgaag tacctttttt gtactccacg ccttcggctt
    15481 taagctgttc ttcggtttta cccacccaag cgatttcagg atcggtgtaa atcacaaacg
    15541 gtacgttgtt gaaatcgata tgcggttttt gaccggcaat gcgctcggca acggcaacgc
    15601 cttcgtcgct ggctttgtgt gccaacatcg ggccgcgaac cacgtcgccg attgcccata
    15661 cattaggcag gttggtgcgg cattcgccat ctactttgat aaagccgcgc tcatcttttt
    15721 ccaagccgac ggcttcagcg ttcaggcctt tggtgtttgg aatacggccg atggcaacga
    15781 tcagtttgtc gaatacttcg gttttggctt cgccggcagc agtttcgtaa gcaacggaaa
    15841 cgcctttgcc ttcagatttg atgtcgccga ttttcacgcc caattcgatg ctcaaacctt
    15901 gctctttggt gaagtatttg aaggcttctt tggcgatttg ttggtcggcg gctgccagga
    15961 aggtcggcgc agcttcaaga atggtaactt ccgcacccac gcggttccat acggaaccca
    16021 tttccaaacc aatcacgcca gaaccgatca cgccgagttt ggcaggtact tcggtcaggt
    16081 tcaatgcgcc ttcgttgtcc aatacgttca cattgtcgat agcgacttgt ggcagcggac
    16141 gcggtacgga accggtggct acgatgacgt gtttggcttc gataacggtt ttctcgcctt
    16201 tgttatcgac ttcgatttgg taagcatcac catttttacc ggcaaaggaa gccgtaccga
    16261 acaggctggt tactttattt ttttggaaca ggaatttcac accgccggtc agtttggtca
    16321 cgatggcatc tttgcgctca atcattttgg ccgcatcgaa ttttacgtcg ccgacagtga
    16381 tgccgtgttc ggcaaactcg tgttgcgctg cgtggaaatg ttcgctggac tgcaacaggg
    16441 ctttggaagg gatacagcct acgttcaagc aagtaccgcc caatgcaggg gcattgcctg
    16501 ctttgttaac gcctgcatcg acacaggcag ttttgaaacc cagttgtgcg gcacggatgg
    16561 cggcaacgta tccgcccggg cctgcaccaa tcactactac atcatattga gacatcgtgt
    16621 catcctttgc ttttagcagt atctcaaaat aaaaacgata cttttcgttt cttttttatt
    16681 ttgaatgact gcgttttgat ggttttaaga aaaagccgtc tgaaagtcca tatctgccga
    16741 ttcattcgaa tcaaatgttt cagacggcct caatcgttcg ttgagcctat tgtagcacga
    16801 tactacatta cgctacatct aaatatagtg gattaaattt aaatcaggac aaggcgacga
    16861 agccgcagac agtacagata gtacggcaag gcgaggcaac gccgtactgg tttaaattta
    16921 atccactata aaaattcaga cggccttttc attaacaaat aaaaggccgt ctgaaacgat
    16981 tacagatcca acaacaggcg ggctgggtct tccaacgcgt ctttaatggc taccaaggtc
    17041 aatacagctt cgcggccgtc aatgatacgg tggtcgtaag acagagccag atacatcatt
    17101 ggacggacaa caacttggcc gttttcaacc acagcgcgct ctttagtggc gtgcataccc
    17161 aaaatcgcag attgaggcgg gttgatgatc ggggtagaca tcatagaacc gaaagtaccg
    17221 ccgttggtaa tactgaatgt accgccggtc agatcttcga tagcgatttt gccgtctttg
    17281 gcttttttcg cgtaatcaac aattgcttgc tcgatgtcgg caatgctcat ttggtcggca
    17341 tcacgcagga ttggcacaac caaaccgcgt gggctgccaa tcgcaatacc gatgtcgaag
    17401 tagccgtggt acacgatgtc tttgccgtca acagaagcat tcacaaccgg gtattttttc
    17461 agggcggcaa cagcggcttt aacgaagaag gacataaagc ccaatttcac gccgtgttct
    17521 ttctcgaatt tttctttgta cttcgcacgc aagtccatga ttggtttcat gttgacttcg
    17581 ttgaatgtag tcagaatggc gttttcttgt tgagaagcca agaggcgttc tgcaacacgg
    17641 gcacgcaggc ggctcattgg tacgcgttct tcaggacgtg cgccggcagg aagtgcaacg
    17701 gcaggggctg cggctgcggc aggtttggca gcggcatttt gtacgtcttc tttcaatacg
    17761 cgaccgtcac ggccggaacc ttgcaatgcg ttcacgtcaa caccggtctc ggcagccagt
    17821 ttggtggcag caggcatagc ggcgttgttt tgtgcagcat caggagcggc agcaggagcg
    17881 gcagccgggg cggcttcggc aggagcggct acgggtgctt cggcagcggc agtagcagct
    17941 gtatcgatgc gtgccaaaac ttggtcggca acaacggttt caccgtcttg cgctacgatt
    18001 tcaaccaata cgccggcttg tggagaaggt acttccaaaa ccactttgtc cgtttcgata
    18061 tcgatcagga tttcgtcacg ggcaactgct tcgccaactt ttttcttcca ttccaagagc
    18121 gtgccttcag atacgctttc agacaacata ggtactttta catcaataat cattttgtgt
    18181 ctccaatggc cttttcagac ggcctgttgt gtgtttttaa ttctgcatat gggcattcca
    18241 tctgagcaga ctcagacgga atgctacttg gtttacaatg ccaaagcgtc ttcaaccaat
    18301 tgtttcaatt gagcaatgtg tttgcttgag tagcccactg caggcgatgc gctgcttgga
    18361 cgaccggcat aagacagttt ttgctcttcg ctaataacat cttcgatgcg gtggcggatt
    18421 tggtagaacg cgccttggtt tttcggctct tcttgcgccc aaaccacaga ttttgcgttc
    18481 ggatatttcg ccagttcggc tttaacctca tcgtatggga acggatacag ctgctcaacg
    18541 cggacaatcg caacatcatc ttccagttta cgttcggctc gaccggcttc caagtcatag
    18601 taaacctgac cggcacacaa taccacgcgt ttcacgctgt cgttgcttgc gcgttcggcg
    18661 gtatcgccga taaccggacg gaaagtcgaa ccttcggtga agttttccag cgggctcatt
    18721 gcccctttga atcgcaacag gcgtttggac atgaaaatta ccagcggttt gcggtatgaa
    18781 cctaagactt gacgttgcaa gaggtggaac atttgcgacg cttcagatgg catgatgact
    18841 tgcatattgt tctcagaaca cagttgcaac cagcgttcta cgcgtgcaga agagtgctca
    18901 gggccttgac cgtcgtaacc gtgcggcagg atggtggtca gaccgcacaa gcgaccccat
    18961 ttggtttcgc ctgaagacag gaattggtca atcgtcactt gcgcgccgtt ggcgaagtca
    19021 ccgaattgag cttcccaaat ggtgagcttg tcaggagcgg agcaggcaaa gccgtactcg
    19081 aacgccatca cggcctcttc gttcaaaatg gagtcgataa ccaggaactc gcccattcct
    19141 tcgcccatat gtcgcagagg aacataagta ccgtcgtccc atttttcgcg tttttgatcg
    19201 tgcaatactg cgtggcggtg cgagaacgta ccgcggccgg agtcctcacc ggagatacgc
    19261 acgccatgac ctttggttac aaggcttgcg tatgccaagg tttcggccat accccagtcg
    19321 atggcttgtt tgccggatgc catggcttta cgcgcttcaa tcacacgttt tgcagtcgga
    19381 tgcagggcaa agccttccgg tacggcggta aatttctcgg taagacgctc aatatccgcg
    19441 gcaggcaaac cggtttcgat gtgttcgcgc caatctttac cttggtattt gctccaatcg
    19501 atttgtgtgc gttggaaatt gctcaacgtt gtttgctcaa cgtgttcgcc tttgtccaaa
    19561 gcatcgcggt aggcttggat gtaaccgtct gcctcggctt gagttaccac accttcggca
    19621 atcagttgct cggtgtacaa agcacgcgca ccaggatgtt gcgatacttt tttgtacatc
    19681 atcggttggg tcaaggtcgg atcatcgccc tcgttgtgac cccatttacg gtagcagaca
    19741 acgtcaatca cgatgtcttt atggaatttt ttgcggtaat ccaaagcggc ttggatagca
    19801 aagcaaacgc gttcgggatc atcgccgttc acatggataa ccggggcgga aaccattttt
    19861 gcgatatcgg tacagtgtac ggttgaacgg gtatcgcgga tatcggaagt ggtaaagccg
    19921 atttgattgt tgatgacgat atgaaccgta ccgccggtgg tataaccgcg cgttttagac
    19981 agattgaatg tcgcttggtt gacacccaaa ccgataaatg cggagtcgcc gtgaatcaat
    20041 acgggcaaga ctttatcgcg gccgttttcg cccaaacgtt tctgcttggc gcgtgcagaa
    20101 ccttcgacta ccgggttaac gatttccaag tgtgacgggt tgaacgccaa agaaacgtgc
    20161 atcggaccat gtggagtggc gatgtcggag ctgaagccca tgtggtattt cacgtcgccg
    20221 ctaggcaatt tgatttcggc acggccttca aactcagcga acaaatctcc gggtttttta
    20281 cccaaaatgt tcaccaaaac attcagacgg ccacggtgcg ccataccgat gatgacttct
    20341 tccacgccgt ctttaccggc gttttgaatc aggtagttca aaccggcaat cgcgctttcg
    20401 ccgccttcga caccgaaacg tttctgaccg acatatttgg tatgcagata acgttccaaa
    20461 gtctcggcag cagtcatctc tttcaagata cggcgttttt gatcggcatt gtaatgcggt
    20521 gtggacaata cgctttcaaa ataattgcgt acccagcggc gctcttcggt attgggaata
    20581 tagatatatt ccaatgcgat gtggccgcag taggtttgtt tgaggttgct gatgatttgg
    20641 gacaaaggca gtttgccgcg attggcaaaa tcgccctcgc ccatattgaa ttgaagcgcc
    20701 atatcggcat ctgacagacc gtggaatttc ggatcgaggg cttcaatatc gcgcgggggg
    20761 atacgtttga gcggatcaag ttgggctgca cccacgcctt ggatacgata ggcggaaatc
    20821 agccgtaaaa cgctgacttg ctttttcagc attgcctcat ccgcaccgcc cgcaacggca
    20881 gatgcaattt tctttttcgc caaagtaaca aatgattcgc gaatcggtgt gtgtgcgaca
    20941 tcgacagcaa ccgtccccgg ctgtttgctc aaatcggtga aatactgctt ccatttttca
    21001 tcaaccgcat cggggttttc caaaaaagcc tcgtacaatt cctcaatgta gggtgcgttc
    21061 gaaccgaaca ggtaagagaa attgagtttt tcgtccatca tggcgtgcgc tcttttcttt
    21121 aaaatataaa caaagggtag aagtccgcgt cctgacggct aaactgatgc cgtctgaaaa
    21181 gacattttat tctaccctat tttaaaccgg attgcttgtc ttgatttcat ccgactacaa
    21241 tcagacggat tcgggaaaca atccggttct ctgatattgc ctgtttgaca atatattaat
    21301 gtatgttacc tctcgcctgg cggcacataa tcgcggcgtt ccgaaccggt ataaagctgg
    21361 cgcgggcggc cgattttcag cgaaggatcg ctaatcatct cgtgccagtg cgaaatccag
    21421 cccacgctgc gcgacagggc gaagatgacg gtaaacattt cggtcgggat gcccagcgcg
    21481 gacaggacga tgccggaata gaaatcgacg tttggataca gtttgcgttc gataaagaac
    21541 gggtctttca gcgcaatctg ttccaattcc atcgccagtt tgaatttcgg actgtcttcc
    21601 aagcccaatt ccttcaaaac ttcatagcag gtttcgcgca taatgctggc acgcggatcc
    21661 atattgcggt acacgcggtg accgaagccc atcagacggt atttgcgttg tttcacacct
    21721 tccatgtatg cggcaacatt agacacatcg ccgatttcgt ccaacatttt caacacggct
    21781 tcgttcgcac cgccgtgcga agcaccccac aggcaggcga taccggcagc aatacaggca
    21841 aacgggttcg cacccgaaga ccctgccaga cggacggttg aagttgaggc gttttgctcg
    21901 tggtcggcat gcaaaataaa gatgcggtcg agcgcgcgtg ccaaaacggg attgggtttg
    21961 tagtcttcac acggcgtggc gaacatcata tgaaggaagt tttcggaata agaaagatta
    22021 ttcttcggat aattgaacgg cagaccgttt gaatagcggt agcacattgc cgcaatggtc
    22081 gggattttgg aaatcaggcg gtaaatcgcg attttgcggt gttcgggatt gctaatgtcc
    22141 aagctgtctt ggtagaacgc agacagcgca ccgaccacgc cgaccatcat cgccatcgga
    22201 tgcgcgtcgc ggcggaaccc ccggaagaac caagtcagct gttcatgcac catcgtgtgg
    22261 cggcggacgg tattgtcaaa ttctgccttt tgctcgggag tcggcagttc gccgtaaatc
    22321 aacaggtagc agacttccaa ataatcggac ttttcggcca gctgctcgat ggggtatccg
    22381 cgataataaa gcaagccttg atcgccgtcg atgtaagtaa ttttagactc acagcttgcg
    22441 gttgaaacaa atccggggtc gaaggaaaac aaacctgtat tttttgtcag cccccgaatg
    22501 tcaaccacat cgtgcccgat gctggcttcc aataccggca gctccaaacc tgcctgaccc
    22561 ggtacgttga gtttgattga tttggacata ttattgctcc tttgcaagtt ataacccacg
    22621 ttactgcatc cgcagcacgt tctttgcctt cagacggctt ttgcattttg aaatctgcct
    22681 gcaggcagat ttcagatgcc tcatgcccgt ctgatttttt caagcatcgg gataaggtgc
    22741 cctttgtccg tttccgaatg cccgttaatc aaggcaagca attcttgatc ttgaaattca
    22801 aggatttcgg aaaactcgga cagctcttta tcgctcaaat gctcgaattc tttttccata
    22861 aacctgccga agattaaatc taattccaac aatccccggc gggtttgaaa acggattttc
    22921 cgtttggcaa tatcgtcaaa aaccatcatt tcttaaacgg cccgtttcaa cataatctct
    22981 ttaatcttac cgatggctcg ggtcggattc aagtgtttag gacatacgtc tacgcagttc
    23041 ataatggtgt ggcaacggaa caaacggtat gggtcgttca gattatccaa acgctcatta
    23101 gtgatggtat cacggctgtc cgcaatgaaa cggtaagcat tcagcaaacc ggacggacca
    23161 acgaatttat cagggttcca ccaaaatgac gggcaggcag tcgaacagca ggcgcacaaa
    23221 atacactcgt acaaaccgtc caactcttta cgctcttcct gagtttgcag acgctctttg
    23281 tccgcatcaa tcggattatc gttgacaaca taaggtttga cggaatggta ttgtttgaag
    23341 aactgggtca tatccacaat caggtcacgg ataacaggca gacctggcag aggacgaatt
    23401 ttaaccggct gtttcaagcc acgcagatcg gtcaaacacg ccaagccgtt tttgccgttg
    23461 atgttcatac cgtcagaacc gcaaatccct tcgcggcagg agcggcggaa agacaaggta
    23521 tcgtcttgtg ctttcaggcg taccaaagca tccaaaagtt tcacgtcggt cggttccaat
    23581 tccaactcgt aacgctgcat ataaggcttg gcatcaacat ccgggttgta acggtaaatt
    23641 tcaaaactca ttttttccat gagaagtgtt ccttttctca tcaactgccc aatgattttg
    23701 tattataaat tcaagcagtt caatgtgggt atggttagat ttcagacgac ctttgaggcg
    23761 aagaccgtct gaaaacgcat caataaacgc gcttggccgg tttgatgtat tccacgctca
    23821 aaggcttggt gtgcaccggt ttgtaggaca aggtattgat atctgaatgg tacagcgtat
    23881 gtttcatcca gttttcatca tcgcgctcag gatggtcgtc tgaagcgtgc gcaccgcgtg
    23941 attctttacg tgcttcggca gacaccaaag tcgctttcgc cacttcaatc aggttatcca
    24001 attccaaagc ctcgatacgc gcggtattcc acactttgct cttgtctttg atttcggtac
    24061 gtttcacacg ctcggcaatc gccatgactt ctcgaacgcc tttgctcaga atctcatcag
    24121 tacggaacac gccggcgtgc aattgtacgg agcgttgcag ttcgcggcgc aatgcatcaa
    24181 cgttttcacc atcagtttga ttgtccaaac gctcgatacg ttggcgggtc agttcgccgg
    24241 cattagcagg caaaggtttc cagtcgcttt gctctttgat gaatttaatc atgctgtcgc
    24301 cggcagcttt accgaatacc actaagtcca gcagggagtt cgtacccaag cggttcgcac
    24361 cgtgtacgga agcacaggcg cactcacctg ccgcatacag accttttaca ggcacttcgt
    24421 attcgtcgcc ttgaggaacg acaacttcgc catggtagtt ggtcggaata ccgcccatca
    24481 tatagtgggt agtcggcaca acgggaatcg ggtctttaat cggatcgata ccagcgaact
    24541 gaatggaaat ctcgcggatg cccggcagtt tttccataat tttttctgcg ccgatatggt
    24601 cgattttcag taagacatgg tctttgtttt taccgcagcc gcgaccttcg tagatttcca
    24661 tcgccatcgc gcgggaaaca acgtcgcgag aagccaagtc ttttacggtc ggcgcatagc
    24721 gttccataaa gcgttcgccg tcggcattca acagaatacc gccctcgccg cgtacgcctt
    24781 cggtaatcaa cacgcccgca cctgccacgc cggtcgggtg gaattgccag aattccatat
    24841 cttccaacgg aatacccgca cgcgcgcaaa tgcccaaacc atcgccggta ttcatatagg
    24901 cattggtaga agaagcataa atacgaccgc caccgccggt agcaaacatc acagctttag
    24961 cgtggaaaat ataaacttcg ccggtttcca tttccatggc ggttacgccg acgacatcgc
    25021 cgttttcatc acgaatcaaa tcttgtgccg tccattccac aaagaattgc gtattggcac
    25081 ggacgttttg ttggtacaaa gtatgcagca tcgcatgacc tgtacggtcg gcaaccgcac
    25141 aggcgcgttc taccgcgcgt ttaccgtgtt cggcagtatg gccgccgaaa ggacgctgat
    25201 aaattttacc gctttccaca cggtcaaaag gcatacccat gtgttccaac tcaattacgg
    25261 cttcaggcgc ggcgcggcac ataaactcaa tcgcatcttg gtcgcccaac cagtcggaac
    25321 ctttcacggt atcgtacatg tgccagtccc aacggtcttc ctgcacatta cccagagagg
    25381 cggaaatacc gccctgcgcc gctacggtat gcgaacgggt cgggaacact ttagacaaaa
    25441 cggcacaatt cagaccggat ttggataatt ggagggctgc gcgtaaacct gcaccaccac
    25501 cgccgacaat cacggcatca aacttgcgaa caggaaaacc catacttacc cccaaattac
    25561 tttaattgaa tacaccaagc agccgaccag ccagacgatg gtggcaacct gcaaaaacaa
    25621 acgcacgccg aagggtttga tatagtccat ccacaaatcg cggataccca cccaagcgtg
    25681 caagaatacg gcgatgaagc tcacttgggt aaatactttt acccaagttt gactaaaaaa
    25741 tgcctgccat gccgaatatt ctttaggcag ggaaaataga accactaaaa gtgcaacggt
    25801 ataaatcaac ataataaccg cagtcgcacg ttgcatcgcc caatcgcgca aaccgtaatg
    25861 ggcaccggtc aatttacgtt ctaccataac aacgctccca aaacgacagt caaaaccaat
    25921 gcagaagcaa atacggcttt agcggtattg cgcgcagtat tcagctcaag gcctttgtgc
    25981 gcatccaaaa ataaaaagcg gataccggcg agagaatggt gcagataagc ccacaataca
    26041 ccgattaaaa ccagcttgac caaaggatgg gaaacaatgg cacggtaagt ttcaaatgca
    26101 gactcttgac tcagggtacc ggacaggaaa tacagcagga aaggcagcat aataaacagc
    26161 ccgaccccgc tgatgcggtg aaggatggaa actatcccgg gtatcggcag acggatgttc
    26221 ggcaaatcca gataaacagg acgcggtttg gcagacatag tcaattcctc tgtagttata
    26281 tatcgactca aaaaccgtaa acccattgct acactgggtt acataaacgt taatttacct
    26341 gttttatccc attttgaaca gtgtatttta aaaacaaact gatagatttt gacaaacacg
    26401 cgtggaaaca gcagattaaa agaaagattt ttagttttgc cgcccccttc ccgacacggt
    26461 atcagcatcc gaccccatcc ccaaaaacaa aatgccgtct gaaaaaattt cagacggcat
    26521 tgttttgcca tatcggtcag gctttcaatt tcgcatcggc atcgcgcaac gcggtacgca
    26581 gaccttcctc gataacggga tggtagaacg gcatatccag catttgcgga acggtcatct
    26641 tcatttgatg cgcccatgcc aacaagtgcg ccaaatgttc ggcggcaggg cctacgattt
    26701 ccgcgccgat aaagcggccg gtggcttttt cggcatacag gcgcatatgg cctttgttta
    26761 ccagcatcac gcggctgcgg ccttggtttt tgaacgatac ttcgccgatg acaaattcgt
    26821 cggcttggta ttgcgcggca acctgcgcgt atttcagacc gacaaagccg atttgcggac
    26881 tggtaaacac cacgccgatg gtgctgcgcc gcaaaccgcc gccgatattc gggtagcggc
    26941 ccgcgttatc gccggcaatc ttgccttggt cggcagcttc atgcagcaga ggcagttggt
    27001 tggacgcatc gcctgcgatg aagatatgcg gaatactggt ctgcatggtc agcgggtcgg
    27061 caacaggtac gccgcgcgca tctttttcga tattgatatt ttccaaaccg atattgtcaa
    27121 cgttcggacg gcggcccacg gctgccagca tatattcggc aacaaatacg cctttttcgc
    27181 catcctgctc ccaatggact tctacattgc cgtctgcgtc gagtttgacc tcggttttag
    27241 catccagatg cagtttcaat tcttcgccga acacggcttt cgcctcgtct gaaacgacgg
    27301 ggtcggaaat gccgccgatg attccgccca aaccgaaaat ttcaactttc acgcccaaac
    27361 ggtgcaatgc ctgacccagt tccaaaccga taacacccgg cccgaacacg gcaacgcgct
    27421 taggcagcgt atcccatgag aaaacgtcat cgttgataat caaacgattg cccaaagact
    27481 gccattgcgg caggatgacg ggacgcgaac cggtagcaat cacgaaactt tttgccgtaa
    27541 tttgagtatg ctcgtcgatt tggacggtat gctcgtcgat aaatttagcc gaacccatga
    27601 tgcgcttgtc ggcaggccac tcttccacat cggcaacgac aaagccgaca aaacggtcac
    27661 gctcggattt aacgcgctgc atgacctctt caccgttgac gacgatgctg tctttgtcca
    27721 aatgcacgcc gaacgggtcg gtatgcaatg cgtgatgacg cgcctctgcg gcggcaatca
    27781 agagtttgga aggcatacag cccacgcgcg cgcaggtcgt gccgaacaca ttgttttcaa
    27841 tcaggtaaac attatccgaa tgtaaacggg cattgcgaaa cgcacccata ccggcagtac
    27901 cgccgccgat tacgacgaca tccgcttgaa tttttttcat aatctttgtc cttccgtttt
    27961 cgtctgaaac attgccgaca cagaacatat cggtttcaga cggcatttaa atattttccc
    28021 aatcaaaaac atcattcaga taaataaaaa caaaatttcg attgagtgca acaatatcca
    28081 aattctctta taaatataga gttattcgga gcggcagtgt agaaccgccc gaataactct
    28141 atccggtata tcagacagtt taatcaaacc gccttaagca aacagctatc agtttttagc
    28201 caagtaagct tccaaatctt cgctgccgcc gatgtattta ccgccgatga agacttgagg
    28261 agcagtcatc ttgccagtaa tggcgcgtac agaagtaacg gttgcatctt tgcccaatac
    28321 gatttcttcg taagacaaac ctttgtcttg caaagcttgt ttggctttag cacagaattg
    28381 gcaacctggt ttagtgaaaa ttgccacaga ttcttgagcc ttccaatcag gagcaacgaa
    28441 tttcagcata gtatctgcat cggatacttt gaacggatcg cctggttctt caggctcgat
    28501 gaacattttt tcaaccacgc cgtcgttaac cagcatggag taacgccaag agcgtttacc
    28561 gaagcccaag tcttctttac cgaccagcat acccatacct tcggtaaatt cgccgttgcc
    28621 gtcaggaatc atgtagatgt tgtcggattc ttcttcggca gaccaagcgt tcattacgaa
    28681 cgtatcgttt acagatacgc agcagattgc gtcaacgccg ttttctttga acgcgccgaa
    28741 caattcgttg taacgcggca ggtgtgaaga agaacaagtc ggggtaaatg caccaggcag
    28801 ggagaatacg actacttttt tgcctttgaa caaatcatcg gtagacacat ctttccaagt
    28861 gtcgccgacg cgggtgcgga atactacgga aggtactttt tgaccggcac gatcttgcaa
    28921 agccatttta agctccttaa tagattggtt tgagaaataa ctgtgaatcg ctgggcattc
    28981 taccccatgc aaaatgattt gtataattta tagttcaaat aatataaatt atttaaaact
    29041 atcaaacaca actccgattt gcctgtttcc cacatataaa gccgaattag aaaaaaacaa
    29101 gctgcaatga aaagattttc cggcaactgc gcccttgcgc cgcaaatcag gacaaaatac
    29161 cgtctgaaag gctttcagac ggtatttatt catacggtct cggcttattt cgccaattcg
    29221 gcacgcaatt tgtgggttac gttcatcatc acttggagtt gttccagagt ttctttccag
    29281 ccgcgtgttt tcaggccgca gtccgggtta acccacagac gttcaaccgg tacaacctcg
    29341 atggctttgc gcaacaggtg ctccacttcg gcttctgtcg gtacgcgcgg gctgtggatg
    29401 tcgtaaaccc ccgggccgat gtcgttcggg tatttgaatt cgccgaacgc ggtcaagagt
    29461 tccatgtcgg aacgtgaagt ctcgatggtg atcacgtccg catccattgc agcaatcgca
    29521 ggcaggatat cgttgaactc ggagtagcac atatgagtgt ggatttgtgt gctgtcttcg
    29581 caaccggcag aggacaggcg gaaggattcg cccgcccagt tcaggtaggc atcccaatcg
    29641 gcgcgtttca gcggcaagcc ttcgcggatg gcaggttcgt caatttggat gactttgatg
    29701 cctgcttttt ccagatccaa tacttcgtcg ttcagagcca gtgcgatttg tttgcacacg
    29761 gtagcgcggg gaatgtcgtt gcggacgaaa gaccattgca gaatggttac agggccggtc
    29821 aacatacctt tcatcgggcg tttggtcagg ctttgtgcgt aagtagacca agccacagtc
    29881 atggcttcag gacggcttac gtcaccgaag ataatcggtg gtttaacaca acgtgagccg
    29941 tagctttgta cccagccgta ttgggtgaat gcaaaaccgc tcaacaattc gccgaagtat
    30001 tcgaccatgt cgttacgctc ggcttcgccg tgtaccagta cgtccaaatc cagtttttct
    30061 tgttcttcaa ccaccaaggc gatttctttt ttcatcgcag cttcgtaatc ggcgacagac
    30121 agttcgcctt ttttgaaagc tgcacgtgct tggcggattt cggtagtttg cgggaaagaa
    30181 ccgatgttgg tcgtcggcag cagaggcagg ttcaaccatg cttgttgcgc tttgatacgg
    30241 tcggcaaatg gagatttgcg ttggtctgcg ttggcaggca aatcagccag gcgtttggca
    30301 acgtctgcac ggtggatttc gctgctgttg gcgcgtgagc cggcggcagc ttggctggcg
    30361 gcgagttctt cggcaacaga atcacggcct tcgttcaatg cagctttcag aacgcgcaat
    30421 tcttgggttt tttgcagggt gaatgccaac caagagtaca ggtcaggttt gttggctttc
    30481 agtttttctt caactgacaa gtcaaatgga gtgtgcagca gagagcaaga gctggagatc
    30541 cacaaacggt cgcccagttt ggcttgcaga gactcaacag ttttcaaaac tttgttcagg
    30601 ttggcgcgcc aaatattgcg gccgtcaatc acgccggcgg acaggacttt gtcgtagtcg
    30661 gcgaacgcgt ccagttgctc gggagcgcgt accaagtcga tgtgcagacc gtcaacaggc
    30721 agggctttca gcaatgcggc gtgttcggca acagaaccga agtaagtgct caacaggatt
    30781 ttggcactta ctttgttcaa agtagcgtaa acgtctttgt aggcttccac ccattcttta
    30841 ggcaaatcga cagccaaagc aggctcgtcg atttgaatcc actcggcacc ggcttcaacc
    30901 aaagcagtca ggatttcaac gtaaacaggc aacagtttag gcaacaggct cagacggtcg
    30961 aattcgacgg cgcctttttc tttacccacc cacaggaaag tcaacggacc tacaacggtc
    31021 ggtttggctt tcagaccgag ggcttgggct tcttgcagtt gttgaacata gtgtttggca
    31081 ttggctttga attcggtatc ggcgtggaat tcaggcacca agtagtggta gttggtgtcg
    31141 aaccatttgg tcatttcgat agcgaattgg tctttgttac cgcgcgccag ttggaagaat
    31201 tgttccaaag acaggttttg gctgtcgaag ccgaagcggg cgggaatcgc gccggtggcg
    31261 acttgcaggt cgaggatgtg gtcgtagaaa gtgaaatcgc ctacggcaac gaaatcggcg
    31321 ttggcagcga cctggtgttt ccagtttttc tcgcgcaagt ctttagcaac agccagcaat
    31381 tcttgctcgc tgatttcttt gcgccagtat ttttcttgtg cgaatttcaa ttcgcggaag
    31441 gcgccgacac gcgggaagcc tgagaaatgt aatgttgtca tgttgaactc tccagttgga
    31501 ttttgtaagg accggttcaa tccgaatcgg ccggtattgt aaatcagaat ggctcggatg
    31561 gggcggtgtt ttgcttttta gacaccttag atgccgtctg aaactgtttg atacggcttt
    31621 aagggcgcac gcctaaaata tggcaaatgg cgtaagtcag ctcgctgcgg ttaagcgtat
    31681 agaagtggaa atctttcacg ccttcgcggg acaggacttt gaccatatcg atggcgatac
    31741 ttgccgccac cagattgcgc gtaccttggt cgtcgtccaa accttcatac atttgcgaca
    31801 gccagcttgg gattttgacg ttggttactt gagccatttt accgagctgc ttgaagttgg
    31861 taacaggcaa aataccgggg acgatttcca catcgatacc caacatcacg cagcggtcgc
    31921 ggaagcgcag gtagcgttcc acatcgaaga agaattgggt gatgacgtgg ttcgcgcccg
    31981 catcgatttt gcgtttcaaa ttaatcaggt cggcttgtgc ggatttcgct tcgggatgca
    32041 cttcgggata tgctgctaca gagatgtcga agtcggcgac ggagcgtaat agcttaacca
    32101 agtcttcggc gtaaaacggt tttttctcat aaccggccgg ctcgtctccg cgcagggcga
    32161 caatgcggcg gatgccgctg tcccaataat ctttggcaat ttggcgcaat tcgtcgggag
    32221 aagcgtcgat accggtcagg tgaggcgcgg cttccaagcc ggtttcctgt ttgatgcgtt
    32281 tgacgatgcc gtgcgtgcgg tcgcgctcgc ctgagtttgc accgtaagtt acggaaacaa
    32341 atttcgggtg caaggtttgc aggcgatgga tggaatccca cagcatggtt tccatttgtt
    32401 cgtttttcgg cgggaaaaat tcgaatgaaa cgttgatgtc gcctttcaaa tcggaaaggc
    32461 tgttatttaa cgcattgatt tcttttgcgt aattcatggc tgcgtacctt ttattatctt
    32521 ttatatgatg tttcaggcaa cataataaag ttgcgccaaa catgagtcaa tttcaaaatt
    32581 ttcagattta tcatgaaaat ttctaatata ttacaaaaat agttaaattt ccttgtacag
    32641 cccttctaaa ctatgctaga gtttgttatg ttataacact gaaagcaagg caatatatga
    32701 aaccgaatat tcatcccgac aattaccgaa ccgtcctttt tttcgatagc agcgccaatg
    32761 aaggctggtt aatccgctcc tgtgccgaaa cacacggaaa aacgatggtt tggacagacg
    32821 gcaaagaata tccgctgttt tcgctggata cttcttccgc atcccatccc gtctatactg
    32881 gcaaacagcg caacgtcaac accgaaggcc gtgccagcaa attcaaccaa cgtttccaat
    32941 ccgtaatgtc atcgtttaga aaggacaaat aatgcaggtt ctgtcttcac tcaaaaccgc
    33001 caaacaacgc caccgcgact gccaaatcgt caggcgcagg ggcaaagttt acgtcatctg
    33061 caaaagcaat ccgcgtttta aagcgcgcca acgctgaccc cctgcccttc ctgcttcggt
    33121 tgtaaatccc ctcctaccgc aagccctttt caaaaaatgc cgtctgaaac agaaaaatgc
    33181 gtttcagacg gcattttttc cgtcaatacg aatccggaat gtgtttgaaa tacagcataa
    33241 acagatacca aaacagcgtc gcggcggccg caccgagcag cacccgggca ctgatttcgg
    33301 caacttcgcg cagggcgcgt ttctgcctgc ggctgaagag gcggttcata atcagcgcgg
    33361 caagtgcgaa aaagaaaaaa atacggaaaa gtctgcctat catgtcggga agcgaaaagt
    33421 atggttcggg cgcggcggca tggggaagga tgtttcagcg cgtcaggctg cttgtccgac
    33481 cgtgtcaaaa agtcatgccg tttgcgatat aataacgctt tttgccgtat ttgttggaac
    33541 aggatatgag tcaaaatgcc tcggtgggca ttgtaatgcc ccaaaaaatt ccgtttgagg
    33601 tgccgctggt tttggaaaac ggtaaaactt tgccgcgttt cgatctgatg attgaaacct
    33661 acggcgagct gaatgctgaa aaaaacaatg cggttttaat ctgccacgcg ctgtcgggca
    33721 accatcatgt tgcgggcagg cattcggcgg aagataaata tacgggctgg tgggacaata
    33781 tggtcggacc tggcaaaccg attgatacgg aacgtttttt cgtggtcggt ttgaataatc
    33841 tgggcggctg cgacggcagc agcgggcctt tgtcgatcaa tcctgaaacg ggtagggaat
    33901 acggcgcgga ttttccgctg gttacggtga aggactgggt aaaatcccaa gccgcgcttg
    33961 ccgattatct cagcatcggg caatgggcgg cggttgtcgg cggcagcttg ggcggcatgc
    34021 aggctttgca gtgggcgatt tcctatcccg aacgcgtgcg ccacgccttg gtgattgcgt
    34081 ccgcgccgaa actgtccacg caaaatatcg cgttcaacga tgtggcgcgt caggcgattt
    34141 tgaccgatcc cgatttcaac gaaggacatt accgcagccg caacaccgtt cctgctcggg
    34201 gcttgcggat tgcccgcatg atggggcaca tcacctatct tgccgaagac ggtttgggca
    34261 aaaaattcgg acgcgatttg cgttccaacg gctatcaata cggctatggc gttgaatttg
    34321 aagtagaatc ctatctgcgc tatcaaggcg acaaattcgt cgggcggttt gatgccaaca
    34381 cctatttgct gatgaccaag gctttggact atttcgatcc ggcggcagat ttcggcgaca
    34441 gcctgacccg cgccctgcaa aatgtgaaag cgaaattctt tgtcgccagc ttcagcaccg
    34501 actggcgttt cgcacccgaa cgttcgcacg aactggtcaa ggcactgatt gccgcccaaa
    34561 aatccgtgca gtatatcgaa gttaaatccg cacacgggca cgatgccttt ttaatggaag
    34621 acgaagccta tatgcgtgcg gtcgccgcct atatgaacaa cgtttataag ggatgtcagc
    34681 aatgaacctg cgcgatgatt tgcaactgat ttacgaccgg atacccgaag gcagccgcgt
    34741 cttggacttg ggctgcggcg acggcgaact gcttgccgca ttggtcgaac acaaaaaatg
    34801 cagcggctac ggcatcgaaa tcgacaccga cagcgtgatt gccgcgatgt cgcgcggcgt
    34861 gaatgttatc caagccgatt tggaagaagg tttaaccgca ttcaacgatc aaagttttga
    34921 tgtgattgtg ttgagccaaa ccatccaagc gatgcagaat accgaaaaaa tcctgcgctg
    34981 cctgatgcgc gttgccaaac aggcgattgt cagcttcccg aatttcggct actggcgcaa
    35041 ccgtgtccaa atcgcactcg gcggacatat gccggtttcc gaacgtatgc cctaccattg
    35101 gtacgacaca ccgaacatcc attggtgtac gctcaaagac tttgatctat tgtgcgccaa
    35161 aaacaacatc cgcgtgcttg agcgcgccgt gatgacgggc aacagacagg tcaaacattt
    35221 ccccaacctt ttgggcagcc ttgcgtttta ccgcgtcggc taagataggc tgagaacctt
    35281 gttggtatag tggattaaat ttaaaccagt acggcgttgc ctcgccttgc cgtactattt
    35341 gtactgtctg cggcttcgtc gccttgtcct gatttaaatt taatccacta taataacgag
    35401 gttcttccgt tgtactaaca agaggccgtc tgaaaaatga aacgtcaggt tttcagacgg
    35461 cctttttaat actggaaaca atagaatgaa aaacaaaacc tcatcacttc tcttatggct
    35521 tgccgcaatc atgctggccg cgtgttcccc gagcaaagaa gataaaacga aagaaaccgg
    35581 cgcatccgcc gcttcgtcct ccgcgtcatc agctccttcc caaaccgatt tgcaaccggc
    35641 cgcatccgcc cctgataacg tcaagcaggc agaaagcgtg ccgccgtcaa attgcaccga
    35701 cctgcaccct gccaccggca ttgacgatct catgcagcaa atcgccgaac acatcgactc
    35761 ggactgtctg tttgcccttt cccatcacga actggaaacc cgtttcggct tacccgacag
    35821 tggctatgac aacatacagc ggctgctgtt tcccgacatc cgccctgaag atcccgacta
    35881 ccatcagaaa atcatactgg caattgaaga cttgcgttac ggaaagcgca cgatcagccg
    35941 gcaggcacaa gatgccttga tggaacagga acgccgcctc cgagaagcga cgctgttgct
    36001 gatacagggc agtcaagaaa cccgcggaca aggcgaggag ccgaaacgca cgcgttattt
    36061 tgaagtttcg gcaacccctg cctattcgag ccggcacaac aacggacttg gcggcaattt
    36121 ccaatacatc ggccaattgc ccggctatct gaaaatacac ggagaaatgc ttgaaaacca
    36181 atcactcttc cggctgtcca accgtgaacg caatcccgac aaaccgtttt tagacatcca
    36241 ttttgacgaa aatggcaaaa tcacgcgtat tgtcgtttac gaaaaaaaca tctacttcaa
    36301 tccaaacttg gggcgaagat aaaaaaatgc cgtctgaaac gttttcagac ggcatttttc
    36361 aatgcaacaa atcaagcgcg tttgcggaat tcgccggtgc gggtatcgat ttcgattttg
    36421 tcgccgtttt cgatgtaaga catcacttgg atttcggtgc ccccgaccag gcgcgcggtt
    36481 ttcatcactt tgcctgaagt atcgcctttg acggcaggct cggtatactc gacttcgcgt
    36541 acgatgatgg tgggcagttc tacggagatg gggttgcctt cgtagaaggt aacttcgcat
    36601 tggtcttcca taccgtcaac gatgaatttc aacgcgtcgc cgatgttgtc ggcttcgatt
    36661 tcgtattggt tgaattcttc atccataaag acgtacatcg gatcggcaaa gtagctgtac
    36721 gtacagtttt tgcgggacag gatgaccacg tcgaatttgt cgtcggcttt gtaaatggtt
    36781 tcggaagccg cgccggtcag caggtttttc agtttcatgc tgactttggc ggaagagcgg
    36841 ccgcctttga tgtattcggt tttttgaacg accataggat cgttgccgac cataaataca
    36901 ttgccggcgc gcagttcttg tgctgttttc attgtgattt tccaatcaaa tcggttgtcg
    36961 taaaacgcgc tattctagcg tattttttga tgctttgaaa caaaggcagc gagtttttca
    37021 ggagctgacg gctgctcgaa aagataacgg ctccaatcct ccgcgccttg ccgccagccg
    37081 ttttgatgtt gttgcagggt ttgccaacat tcgaggcgtt gcgtggcgga taaagcctct
    37141 ccgccgttga ggtcgtctga aaggcggcgg tgtaccgatg cggtttcggg cgagtaaaat
    37201 ccatgcgcct tatcccaaaa ggcgtggagt ttatcgagat ggacattctc gtcttgcggg
    37261 tagatgtgcc aaaagaaggg tttgccggca agctgggcgc gcacgaaact gtcttcgccg
    37321 cgtatcacgg cgcagtcggc gaggtgcagc agtttgtcga agtcctgttg cggcacgaaa
    37381 gggattttga cgaggcggac ggatgccgtc tgaaaaacat cgccgtcgtt ttgcagggca
    37441 tcttgcggaa taacgccgct ttgtttgagg ctgtcgatga tttgcgtccc cgccagcaac
    37501 agtgtcatcg ggctgcctgc ctgtcgccac atttccagcc actttgccca aacatcgctc
    37561 cgatagccga aaagcagcca ttcgggggcg tttttttcgg gaagcatcag cctcttgcgc
    37621 aaggctccgc tatcgaaacg gacggcttcg cagtaatcgc gttcgcgtat cagtccgccg
    37681 cttttttcgc tgaagcccat aaaccaaaaa tatttttgaa caccttcctg cggcgaaggc
    37741 atcaggtgca gcctttcatt gctttcctcc gcgctcaaat attcccaatt cagccaaagc
    37801 ggcttgtgtc ggcggataat gtgcagcaca ttttcgggca ggtcgcaggc aaaagtttcg
    37861 atgacgacat cgggaacagg cgcggtatca atgtctgccg catcggaatg ccaagtgcgg
    37921 acatcaatat cctgatgaac acagggaaca tcgggcaaat cagggcaaag cgcacgcaag
    37981 gcggacacat cgtccgtcca caaatgcacc cgccaaccga gttcgcgatg caaaacacgg
    38041 gcaagccgca gggaaacgcc gatatcgccg aaattgtcga tgaccttgca aaaaatccag
    38101 cagacaaaag gaggagtatt cataggaata acggaaacgg gctaaaacgc gccgaaatag
    38161 tggaacagat acacaaccag caaaacaatc ggaataaaca gaaacatagt ttgacgtttc
    38221 aaatcaaact cttgcttcaa atgatgcttg atggtgtcga aaaggacgaa tttgaacaaa
    38281 aacgtcgctt cgcaggcaag gatgatgccg ttgatcagat gctggagttt gtcttcgggc
    38341 ggattggcgg cgaaaacggc aagcgtcgcc aacgggatga ggcagacggc aagccaacgg
    38401 acaagcaggt ttcgagacat tgtaaggctt tcaaaaaaag caaaacgcca ttatcgcccc
    38461 aagcgcacgc cttgtctaac ggacgcaccg gagatgccgt ctgaagcctt gccgcaggaa
    38521 aacaccgcac cgccgaacct ttaaatacgc ccgagacggg cgttccccga aaaatcggac
    38581 gctgccgccg atgcctgagg acaaggcggc agcaggcacg ggcatattga aaacccggcg
    38641 cgtcggtttg aa
//
LOCUS       DAWFJR010000010        36896 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWFJR010000010 DAWFJR010000000
VERSION     DAWFJR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 36896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36896
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.010"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(479..1414)
                     /locus_tag="WGR04_000437"
     CDS             complement(479..1414)
                     /locus_tag="WGR04_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ0794061.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPEAIDYVDERKGVP
                     LAAMRHILMYTEAAASHAYLFEHDLNKFKQYAYVAGKLGVLLSVNAEDPEPFFFPCDM
                     LNIQNPMFLMLMSDSPQLREFLVRNIDNIANDTEAFVNRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLVHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDQEIAPRDLIVYDPLPAAEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
     gene            complement(1417..3555)
                     /locus_tag="WGR04_000438"
                     /pseudo
     CDS             complement(1417..3555)
                     /locus_tag="WGR04_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
     gene            complement(3662..3925)
                     /locus_tag="WGR04_000439"
     CDS             complement(3662..3925)
                     /locus_tag="WGR04_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794062.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            complement(3944..4810)
                     /locus_tag="WGR04_000440"
                     /pseudo
     CDS             complement(3944..4810)
                     /locus_tag="WGR04_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(4906..5178)
                     /locus_tag="WGR04_000441"
     CDS             complement(4906..5178)
                     /locus_tag="WGR04_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794063.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNDMD"
     gene            complement(5365..5853)
                     /locus_tag="WGR04_000442"
     CDS             complement(5365..5853)
                     /locus_tag="WGR04_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794064.1"
                     /translation="MKKDIFYCEQWSYGYKKLHKPFSEKQAEEKHLKGELYTAVIGSA
                     TQPEYVITLREEVGFFSVHFFDKFGRDYLTHQFQKYSNSNYYFLSMAVWRDYITLESH
                     DLAEGYTYFFNENTDDCYVLKEDFINNERYEKTELYSQKDKVILFPKFGEYDLVLNPD
                     II"
     gene            complement(5850..8351)
                     /locus_tag="WGR04_000443"
     CDS             complement(5850..8351)
                     /locus_tag="WGR04_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0794065.1"
                     /translation="MLKTPPMLAAELSGKTGVSISAPYANENSRIILSAADIAAENGK
                     IKLQSYGDQSYYAGQGELYTLERRSYKTGKWYNRKHITEVKEHKNAKPDAVTLSASQG
                     IDIKSGGSIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYS
                     KAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARAD
                     AKIILEGIKTTIHNETVSSSKSALWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYI
                     VDIPKGNLKTQIETLTKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEGMTPAAAA
                     VVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGAAAGTAAGTGVAAGTAATTGVAAGTS
                     AAAITTAAGKAALASLASQAAVSLINNKGDINHTLKELGKSSTVRQAATAAVTAGVLQ
                     GISGLNTQAAEAVSKHFHSPAAGKLTANLINSTAAASVHTAINGGSLKDNLGDAALGA
                     IVSTVHGEVASKIKFNLSEDYIAHKIAHAVAGCASAVANKGKCRDGAIGAAVGEMVGE
                     TLLDGRDVGKLSPQERQKVIAYSQIIAGSAVALVKGDVNTAANAATVAVENNSLLARR
                     RVNIRWTSRQELEHEYAILEIQAITNQIRRLDPKFNGIAIMRNPREPWTRHDVQTYRQ
                     YYNQLRESRGFAVDPIYRIRINNGNEFNRIMSSKYPYNELYVANPKSATGYFRVDSYN
                     PATEEIISRKFTQFSQIQESTGIGYIKEAVRKYSPGAVISNVPSTPTTIRGRKLEGKL
                     ILEVPAQVNPIPQSVLRAAQEENVIIRDTTGRIYK"
     gene            complement(8601..8984)
                     /locus_tag="WGR04_000444"
     CDS             complement(8601..8984)
                     /locus_tag="WGR04_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794066.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
     gene            complement(8989..11040)
                     /locus_tag="WGR04_000445"
     CDS             complement(8989..11040)
                     /locus_tag="WGR04_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0794067.1"
                     /translation="MRKVCASARTRSMIYGSMPSEKLTIFQTAFVMQVNIQIPYILPR
                     CVRAEDTPYACYLKQLQVTKDVNWNQVQLAYDKWDYKQEGLTGAGAAIIALAVTVVTS
                     GAGTGAVLGLNGAAAAATDAAFASLASQASVSFINNKGDVGKTLKELGRSSTVKNLVV
                     AAATAGVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNLEANI
                     LAALVNTAHGEAASKIKQLDQHYIVHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEIV
                     GEALTNGKNPATLTAKEREQILAYSKLVAGTVSGVVGGDVNTAANAAKVAIENNLLSQ
                     EEYALREKLIKKAKGKGLLSLDWGSLTEQEARQFIYLIEKDRYSNQLLDRYQKNPSSL
                     NNQEKNILAYFINQTSGGNTAWAASILKTPQSMGNLTIPSKDINNTLSKAYQTLSRYD
                     SFDYKSAVAAQPALYLLNGPLGFSVKAATVAAGGYNIGQGAKAISNGEYLHGTVQVVN
                     GTLMVAGTVSAQAAISAKPAPVTRYLSNDSAPALRQALTAESQRIRMKLPEEYRQIGN
                     LAIAKIDVKGLPQRMEAFSSFQKGEHGFISLPETKIFKPISVDKYHNIASPPRGTLRN
                     IDGEYKLLETIAQQLGNNRNVSGRIDLFTELKACQSCSNVILEFRNRYPNIQLNIFTG
                     K"
     gene            complement(11075..11449)
                     /locus_tag="WGR04_000446"
     CDS             complement(11075..11449)
                     /locus_tag="WGR04_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794068.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDELEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
     gene            complement(11727..12104)
                     /locus_tag="WGR04_000447"
     CDS             complement(11727..12104)
                     /locus_tag="WGR04_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794069.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            complement(12113..13801)
                     /locus_tag="WGR04_000448"
     CDS             complement(12113..13801)
                     /locus_tag="WGR04_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0794070.1"
                     /translation="MRSMIYGSMPSEKLTIFQTAFVMQVNIQIPCMLYRRGSVKPPLF
                     EAPRLLPSFTDPVVPKLSAPGGYIVDIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNVN
                     WNQVQLAYDKWDYKQEGLTRAGAAIIALAVTVVTAGAGVGAALGLNGAAAAAADAAFA
                     SLASQASVSLINNKGDVGKTLKELGRSRTVKNLVVAAATAGVSNKLGASSLATWSETP
                     WVNNLNVNLANAGSAALINTAVNGGSLKDNLEANILAALVNTAHGEAASKIKGLDQHY
                     VAHKIAHAVAGCAAAAANKGKCQDGAIGAAVGEIVGEALVKNTDFSDMTPEQLDLEVK
                     KITAYAKLAAGTVAGVTGGDVNTAAQTAQNAVENNAVKAVVTAAKVVYKVARKGLKNG
                     KINVRDLKQTLKDEGYNLADNLTTLFDETLDWNDAKAVIDIVVGTELNRANKGEAAQK
                     VKEVLEKNRPYIPNKGAVPNMSTYMKNNPFGKQLAQISEKTTLPTQQGQSVFLVKRNQ
                     GLLKTGDRFYLDGQHKNHLEVFDKNGNFKFVLNMDGSLNQMKTGAAKGRKLNLK"
     gene            complement(13861..14370)
                     /locus_tag="WGR04_000449"
     CDS             complement(13861..14370)
                     /locus_tag="WGR04_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794071.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
     gene            complement(14377..20313)
                     /gene="hrpA"
                     /locus_tag="WGR04_000450"
     CDS             complement(14377..20313)
                     /gene="hrpA"
                     /locus_tag="WGR04_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion exoprotein HrpA"
                     /protein_id="HEZ0794072.1"
                     /translation="MNKGLHRIIFSKKHSTMVAVAETANSQGKGKQAGSSVSVSLKTS
                     GDLCGKLKTTLKTLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQ
                     TPNGRGLSHNRYTQFDVDNKGAVLNNDRNNNPFVVKGSAQLILNEVRGTASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGA
                     AGWNDKGEADYTGVLARAVALQGKLQGKNLAVSTGPQKVDYASGEISAGTAAGTKPTI
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADG
                     TEASPTYLSIETTEKGAAGTFISNDGRIESKGLLVIDTGEDIGLRNGAVVQNNGNRPA
                     ATVLNAGRNLVIESKANVNNAKGSTNLSTGGRTVIKEASIQTGTTVYSSSKGNAELGN
                     NTRITGADVTVLSNGTISSSAVIDAKDTAHIEAGKPLSLEASTVTSDIRLNGGSIKGG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGSLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTAL
                     QGNIVSDGLHAVSADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNIT
                     SSSGDITLVAGNGIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSD
                     RALSIENTKLESTHNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLV
                     ANGVLALNARYSQIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPL
                     AGRLNIEAGSGTLTIEPANRISAHTDLSIKTGGKLLLSAKGGNAGAPSAQVSSLEAKG
                     NIRLVTGETDLRGSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKE
                     LEQQIAQLKKSSPKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQN
                     IDLISAQGIEISGSDITASKKLNLHAAGVLPKAADSEAAAILIDGITDQYEIGKPTYK
                     SHYDKAALNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLE
                     AGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTT
                     RFNAPAGKVTLVAGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNYSKNELNETKL
                     PVRVVAQTAATRSGWDTVLEGTEFKTTLAGADIQAGVGEKARADAKIILKGIVNRIQS
                     EEKLETNSTVWQKQAGRGSTIETLKLPSFESPTPPKLTAPGGYIVDIPKGNLKTEIEK
                     LAKQPEYAYLKQLQVAKNVNWNQVQLAYDKWDYKQEGLTRAGAAIVTIIVTALTYGYG
                     ATAAGGVAASGSSTAAAAGTAATTTAAATTVSTATAMQTAALASLYSQAAVSIINNKG
                     DVGKALKDLGTSDTVKQIVTSALTAGALNQMGADIAQLNSKVRTELFSSTGNQTIANL
                     GGRLATNLSNAGISAGINTAVNGGSLKDNLGNAALGALVNSFQGEAASKIKTTFSDDY
                     VAKQFAHALAGCVSGLVQGKCKDGAIGAAVGEIVAESILGGRNPATLSDAEKHKVISY
                     SKIIAGSVAALNGGDVNTAANAAEVAVVNNALNFDSTPTNAKKHQPQKPDKTALEKII
                     QSIMPAHAAGAMTNPQDKDAAIWISNIRNGITGPIVITSYGVYAAGWTAPLIGTAGKA
                     AISTCMANPSGCTVMVTQAAEAGAGIATGAVTVGNAWEAPVGALSKAKAAKQAIPTQT
                     VKELDGLLQESKNIGAVNTRINIANSTTRYTPMRQTGQPVSAGFEHVLEGHFHRPIAN
                     NRSVFTISPNELKVILQSNKVVSSPVSMTPDGQYMRTVDVGKVIGTTSIKEGGQPTTT
                     IKVFTDKSGNLITTYPVKGN"
     gene            complement(20423..22165)
                     /gene="hrpB"
                     /locus_tag="WGR04_000451"
     CDS             complement(20423..22165)
                     /gene="hrpB"
                     /locus_tag="WGR04_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ0794073.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTVRKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLPSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLVHK
                     TDLTDATGTETESGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRFHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGGTIPGTSRMKIITAGLDAAAPFMLGKQQF
                     SYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLSGERGFYWQNTLGWHFHPRH
                     QFYLGADYGRVSGESAQYASGKRLAGAVAGFRGSHKVGGIFAYDLFAGKPLHKPEGFQ
                     TANTVYGFNLNYSF"
     gene            complement(22226..22465)
                     /locus_tag="WGR04_000452"
     CDS             complement(22226..22465)
                     /locus_tag="WGR04_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0794074.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTNL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGEFCKGLIL"
     gene            complement(22749..23051)
                     /locus_tag="WGR04_000453"
     CDS             complement(22749..23051)
                     /locus_tag="WGR04_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794075.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            23061..23168
                     /locus_tag="WGR04_000454"
                     /pseudo
     CDS             23061..23168
                     /locus_tag="WGR04_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(23417..23572)
                     /locus_tag="WGR04_000455"
     CDS             complement(23417..23572)
                     /locus_tag="WGR04_000455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794076.1"
                     /translation="MGMVSSDLAKSNEPVYLVKIKPDKSTITAYGEEKPLQIGMTLEA
                     DILHEKR"
     gene            complement(23518..24144)
                     /locus_tag="WGR04_000456"
                     /pseudo
     CDS             complement(23518..24144)
                     /locus_tag="WGR04_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246842.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
     gene            complement(24205..24558)
                     /locus_tag="WGR04_000457"
     CDS             complement(24205..24558)
                     /locus_tag="WGR04_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794077.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDLVAI
                     PAAAAAVYYLPKNAYGAAGANGVYNVTRDWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            complement(24787..25935)
                     /locus_tag="WGR04_000458"
                     /pseudo
     CDS             complement(24787..25935)
                     /locus_tag="WGR04_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            26297..26578
                     /locus_tag="WGR04_000459"
     CDS             26297..26578
                     /locus_tag="WGR04_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794078.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            26575..27618
                     /locus_tag="WGR04_000460"
     CDS             26575..27618
                     /locus_tag="WGR04_000460"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ0794079.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDALRLLEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEAGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSNGICPETVLRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLGAIPLLP"
     gene            complement(29040..31082)
                     /gene="recG"
                     /locus_tag="WGR04_000461"
     CDS             complement(29040..31082)
                     /gene="recG"
                     /locus_tag="WGR04_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ0794080.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESEPLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQVREIAPMLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(31168..31611)
                     /gene="secB"
                     /locus_tag="WGR04_000462"
     CDS             complement(31168..31611)
                     /gene="secB"
                     /locus_tag="WGR04_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ0794081.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDADLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(31632..31889)
                     /gene="grxC"
                     /locus_tag="WGR04_000463"
     CDS             complement(31632..31889)
                     /gene="grxC"
                     /locus_tag="WGR04_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ0794082.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            32102..33595
                     /gene="rng"
                     /locus_tag="WGR04_000464"
     CDS             32102..33595
                     /gene="rng"
                     /locus_tag="WGR04_000464"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ0794083.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(33630..34880)
                     /locus_tag="WGR04_000465"
     CDS             complement(33630..34880)
                     /locus_tag="WGR04_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ0794084.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRIFVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRING
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQPAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFHQV
                     LQELMPLALEKRQDIGVAVGGDVEVSADETEIYTLIKTFADNAVRYTPPEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERARVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGHL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(34876..35051)
                     /locus_tag="WGR04_000466"
                     /pseudo
     CDS             complement(34876..35051)
                     /locus_tag="WGR04_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(35269..36582)
                     /locus_tag="WGR04_000467"
     CDS             complement(35269..36582)
                     /locus_tag="WGR04_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ0794085.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPFELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
BASE COUNT         8860 a         9233 c         8417 g        10386 t
ORIGIN      
        1 tcttcctctt atcgtagtag gtgtacttgg aacattggaa atgacaaagt gagtgcgcaa
       61 agccatctga aggcgatgtg tttgaacctg ttgaaagcgg ccaacaggct aaatgcgccc
      121 gctgccgcct aaaaggcagc ccggatgcct gattatcggg tatccgggga ggattaaggg
      181 gatatttggg taaaattagg aggtatttgg tacgaaaaca gccgaaatcc tgtgtttggg
      241 tttcggatgt cggggaaggg cttttttgca aaggtctcaa ctcatgttat tgcaaagatc
      301 tcatgccttg ttttgccagc aggttgagtt cttctccggc aatcagcgtg gtaaagcccc
      361 gccacaacac agatctatgt actgtggaag ggcttcaatt tatagctttc gaaaatcttt
      421 cagctttaag acggcctaaa gttgtaatct ataaaacagg agctcagagt tttacggctc
      481 actctcgctt cgcgttacca aataccagct tatcggtttt gaacgtatag taatctatcc
      541 aatcctgcag atattcatac gggtaagaca agtcgtactg tttcataaaa tcgaagatgt
      601 cctggtattc ggctgccggc agcggatcgt aaacaatcaa atccctcggt gcgatttctt
      661 gatctatgcc caaatcgaaa ccgtgcatgg atgcgatttt ggcgtaggta acgatttgcg
      721 gctgcaggta gaaatcgaaa taggacaatg tttctttggc agccatacgc gcggtttttt
      781 tatcgaaaag cggctctaag gcggctttca tcgcctcggc atcctgttcg gcgaaagcga
      841 ggaagaagcg gtaatcgtac aaccgctttt gcagccattt gctaggggtg gggtgcacca
      901 agactttctc gctacgttgt ttcaaccgat caagctgctt accctccacc atcagcagag
      961 tattgtaaat catatgacgg ttgaggtcgt atcggtttac gaaggcttct gtatcgttgg
     1021 cgatgttgtc gatattgcgc accagaaact cgcgcagctg tgggctgtcg ctcatcagca
     1081 tcagaaacat cggattttga atgttgagca tgtcacaggg aaagaagaag ggttcggggt
     1141 cttcagcatt tacactcagc aaaaccccca gctttcctgc aacataagca tattgcttga
     1201 atttattaag atcatgttca aatagatagg catgggaagc cgcagcttca gtatacatta
     1261 aaatatgtct catcgctgca agcggcacac cttttctttc atctacataa tctatagctt
     1321 cagggcaacc gctattgaaa ttagcagtat tgcctatgat tacattagta atatgctcat
     1381 accatttttg ggtggtcatc atattgtgcc ccattgttat ctccttatat tggttttaga
     1441 aggaactttg acagaaagaa taacggcctt acctgtttga cgatcaacgc ctgctattgc
     1501 tgtttttaat ttgccttccc gctctgcttt gaagacagca gctttagcag gactaccatc
     1561 aggtaagcta tttgcaactt gtttaatcca atcctcactc atttgcgtat atccacccgc
     1621 accattcgga ttcagctgta ccgtaccgtt cctaatctgc ttactttcta caatcaaaac
     1681 gacactacca tcggcagcct gccatacatg atcaaaaccg ttatttccgc cgtatttgcc
     1741 gcccgaaagc actctgaagc cattttgttt agctaaagag tcaaataact gctcggtcat
     1801 tttacctgtc gtgtcccttc cttcgccaat agctttcagc aattgtttgt cagttgctgc
     1861 agtaaccctt aattcagttt gaccgatttt gattttggtt ttactgacat agccctgtgc
     1921 agtggcaacg gttgctgcct tgtccaaggc ttttttcatg ccttgtaaat tttttgccgc
     1981 tttcgctact ttataggcct gtatcgattc accgataccc ggaaccacac ccagcaaagc
     2041 aaacaggtga tcggcagcgg tctgtgcttg tacaaaactc tgtatatcac cgataattgg
     2101 aataaatcct gtctgaaaaa gcaaaacttc tcgatattct tcaggatttt cacgtatcca
     2161 tttttgttgt tccttttgaa attcatcaca ctttgcacca acgcatctag gatacaaagt
     2221 attattctcc accgctatct gtgccgcatt cgccgctgta ttcacatccc cgcctacaac
     2281 ggcagacgca gtgccggcaa ccagttttga ataggcagta atcttcgctt tagctttttc
     2341 gatttcggtc gcactcatac gactgaaatc agtattctta accaaagcct caccaacaat
     2401 ctcaccgact gcagcgccta tcgcaccatc ctgacactta cccttattcg ccgccgctgc
     2461 cgcacagccc gctatggcat gggctatctt gtgggtaatg tagtgctgat ccaactgttt
     2521 gattttactg gctgcctctc catgcgcagt attcaccaaa gccgcaagga tattcgcttc
     2581 cagattgtct ttcaggctgc cgccgttgac agcggtatta atcagtgcgg cactgcccgc
     2641 attggccagg ttgacggtca ggttgttgat ccactgctta tcgctgacat tgttcagtgc
     2701 cgaagcaccg attttgtcgg ctacgcctgc ggtagcgacg gcaaccatca gatttttcac
     2761 cgtgctgctt ctgcccagct ctttcagggt gttaccgata ttgcctttgt tgttgatgaa
     2821 cgatacggaa gcctggctgg ccaaagaggc aaatgctgca tcggttgcgg cggcggccgc
     2881 accgtttaat cccaatacgg ctccggttcc tgcgcctgag gtgaccacgg taacggccag
     2941 tgcgataatt gcggctccgg ctccggttag gccttcctgt ttatagtccc atttgtcgta
     3001 agcgagctgt acttggttcc agttcacgtc cttgaccgtc tgaagctgtt tcagataggc
     3061 atattcgggc tgtttggcca gcttttcgat ttcggttttg aggttgcctt tggggatgtc
     3121 ggcgatatag ccgccgggag cggtcagctt aggcagtgcc ggcccttcaa agctcggtag
     3181 cttcagcgtt tcaaccgtgc tgccgcttcc ggcctgcttt tgccatacgg tcgagttgga
     3241 ttccagcttt tcttcggttt ggatgcggtt aacgatgcct tttaggataa ttttcgcatc
     3301 ggctcgggct ttttcaccca cccctgcctg tatgtcggct ccggaaaggg tggttttgaa
     3361 ttcggtgcct tcgagtacgg tatcccagcc ggaacgggtt ttggctgttt gggcgataac
     3421 gcgtacgggc agtttggttt cgttcagctc gtttttgctg taattgcttt tacccacttt
     3481 gatgccgatg aaacgggtac ttttctgaac attcaattgg tgttggtgaa tgccttcggc
     3541 tgccagcagt tgcattgagg ccgtctgaaa aacggatatt cagacggcct attgctactt
     3601 taaataatat ttgggtttaa aatatcatcg tatttcccaa attcggggaa cagaaaatca
     3661 attatttaaa tttttcatcc tcataatatg ggtgatcaaa aagctctcct tcccacatat
     3721 ttatttcttc tatgccttta tctttcataa actcatgata atcataaaaa tcttttccat
     3781 attgtccgta aaaaattttc atatccccaa gccacatagc ataacattca tcgcataaaa
     3841 tgacctcccc agagaaatat ttaggctttg catgataaag gtagccttgc tcacatcttg
     3901 ggcaaatttt aatttcttta tccataagag attcctttct taattatttc tgaggatatg
     3961 cagaaatgat tttatttgtt cctttttgca caacaatccg cacttttgtt tctccttttg
     4021 ttccaatagg tttcttcata tctacaagat atacggatga atcatttggc aaaggatttc
     4081 ctttcattct ccaagcttca tcaaccaaag gcaaaacttc tttacgggac acattaaaaa
     4141 cagaatgaac aggtttgttt ggatttggaa ttgtatgggc taaaacatgt ttaatcctat
     4201 taccatgctt agcatctaac ccataatcta accccgccgg tgttgtccaa accttattaa
     4261 ccttatcata agtaagcttg attccggtct gcttctgaat ttcctgtatg gctttagccg
     4321 tatcaactgc ttcgtctccc gccttcagaa cataagcccc gcctcggctg attttagtcg
     4381 cacctgccgg aacaaaggga acggcagctg cggcggcatc cacaccaaaa tcaatcgccg
     4441 cttctcgagc catctgctta tcgccaatag atttggcgta aatccattta ccgccgtccc
     4501 atacgaggtt gccaatatcc caaataatat ctaatgcatt atttttaaca gctgtttcgg
     4561 aagcatttgc cgccacatca acattgcctc ctgtaacacc tacagtcgct ccggctacca
     4621 attttgcata cgcagtaata ttcgctttag ctttttcaat ttcagaagca gtcataccgc
     4681 tgaaatcggt attcttaacc aaagcctccc cgacaatctc ccccacgacc gcaccaatcg
     4741 cgccgtcctg acacttgccc ttattcgccg ccgctgccgc acagcccgct atggcatggg
     4801 caatcttgtg ggtggtatat cgctgccagc cctctctgtt gataatcttg cgtatgatta
     4861 cgccgtttga tggctaatcg tacctacgca ggcttaaaat ttccatcaat ccatatcatt
     4921 tgagttttca tcttcataat atggatgatc aaacaaatct ccttcccaaa tacattcact
     4981 cgttatacct tgggattcca tgaaaggaac ataagaataa aaatcttttt catattctcc
     5041 ataaaatata ttcatacctt tgagccatac tgcatcgcat tcatcacaca gaataatttc
     5101 atcatgaaga tatttaggtt tcgaatggta gagatagcct tgttgacaag caggacacat
     5161 tttcattctc ttattcatga ttactccata taatcgattt atttttgtgg gtaagcagta
     5221 gtgatttgat tgctgccttt agtctgtaca acaatacgta tatagcaacc tgataatttc
     5281 ctggttttta tcagctttat cctcagattc caaacccaat aataaaagcc gtctgaaatt
     5341 ttttcagacg gccttaaaac tcaattaaat aatgtccgga tttaacacca aatcatattc
     5401 gccaaacttt ggaaatagaa ttaccttatc tttttgggaa tataattctg ttttttcata
     5461 tcgctcatta ttaataaaat cctctttcaa aacatagcaa tcatccgtat tttcattgaa
     5521 gaaataagta tatccttctg ctaagtcatg agattccaaa gttatataat ctctccatac
     5581 agccatagaa agaaaataat aattcgaatt ggaatatttt tgaaattgat gggttaaata
     5641 atcccttcca aatttatcga aaaaatgtac cgaaaaaaaa cctacttcct ctcgcaaggt
     5701 aattacatat tcaggttgtg tcgccgaacc tattacggca gtatataact cccctttaag
     5761 atgtttttcc tcagcttgtt tctcagaaaa aggcttatga agtttcttat aaccataaga
     5821 ccactgctca caataaaaaa tatctttctt catttgtaaa tccttcctgt tgtatctcta
     5881 atgataacat tttcttcttg tgccgccctt aatacagatt gtggaattgg attgacctga
     5941 gcaggaactt ctaaaataag ttttccttca agctttcttc ctcttatcgt agtaggtgta
     6001 cttggaacat tggaaatgac agcaccaggg ctatattttc taacagcctc cttgatataa
     6061 ccaatccccg tactttcttg gatttgagaa aattgggtaa attttcttga aataatttcc
     6121 tctgtcgcag gattatacga atctacccta aaataccccg tcgccgattt aggattggct
     6181 acataaagct cattataagg gtattttgat gacatgatac ggttaaattc attgccgttg
     6241 tttatcctga ttctataaat tgggtcaaca gcaaagcctc tggattccct taattgatta
     6301 taatattgcc tgtatgtttg tacatcatgt cttgtccacg gctctctagg attcctcata
     6361 atagcaatcc cgttaaattt cggatccagc cttcggattt gattggtaat ggcctggatt
     6421 tcaagaatgg catattcatg ttccaattct tgtcgcgaag tccaacgtat atttaccctc
     6481 ctgcgagcta aaagactatt attctccact gccacagtag ccgcattcgc cgccgtattc
     6541 acatcccctt taaccaatgc cactgcgctg cctgcgataa tctgcgagta ggctatgact
     6601 ttttggcgtt cttggggtga cagtttgcct acatcgcgtc cgtccaacag ggtttctccc
     6661 accatctcgc cgactgccgc gccgattgcg ccgtcccgac atttgccttt atttgctacc
     6721 gccgatgcac agcctgctac ggcatgggct atcttgtggg caatgtagtc ttcgctgaga
     6781 ttaaatttga ttttgctcgc tacttctccg tgtacggtac tgactatcgc acccagtgcg
     6841 gcatcgccca agttgtcttt caggctgccg ccgttgatgg cggtatggac acttgcggca
     6901 gcggtgctgt tgatcaggtt agcggtcagt ttgcctgctg cgggactgtg aaaatgtttg
     6961 ctgacggctt cggctgcttg ggtgttcagc ccgcttatgc cctgcagtac gcctgcggtt
     7021 acggcggcgg tggcggcctg tctgacggtg ctgcttttgc ccagttcttt cagggtatgg
     7081 tttatgtctc ctttgttgtt gatgagggaa actgcggctt ggctggcgag actggccagt
     7141 gcggctttgc ctgcggctgt ggtgatagct gcagctgatg tgcctgctgc tactccggtt
     7201 gtggctgccg ttcctgctgc tactccagtt ccggctgccg ttcctgctgc ggctcctccc
     7261 gctcctgcgc ctgccgcgcc cgccgttccg gcggctgccg gggcggacag tgcgccgtag
     7321 gtcaatacgg ttacgacgat aacgacgaca gctgctgctg cgggtgtcat gccctcctgt
     7381 ttgtagtccc atttatcgta agcaagctgc acctgattcc agttgatgtt tttcgcaact
     7441 tgaagttgtt tcaaataagc atactcgggc tgcttggtga gggtttcgat ttgggttttc
     7501 agattgcctt tgggaatatc gacaatgtaa ccgccgggtg cggacagtac gggcgcaacg
     7561 ggaccggtga aactcggcaa ttgcaaggtt tcgatgttac tgccccgtcc tgcctgtttt
     7621 tgccatagag cagatttgct gctgctcacg gtttcgttgt ggatcgtggt tttgatgcct
     7681 tcgaggataa tcttggcatc tgcccgtgcc tgctcaccta cgcctgcgcg tatggttgcg
     7741 ccacccagtg tggtttcaaa ctgtgtgcct tgcagtttgg tatcccagcc cgattggagg
     7801 ttggctgatt ctgcaactac ccttgagggc agcgcggttt tcatgacttg ggtggtggtg
     7861 tcgtgtgctt tgctgtagct gatgccgaga aatctgcgcc ttttttggct gtctagtttg
     7921 tcgtagttga gctcttctac ggcatagagt gtcaatttcc gcccggcttc gatgttaatg
     7981 ctgcctttgg gggcatcgaa tgcggtggcg taggcgtcga tgctgccacc agatttgatg
     8041 tcgatgcctt gggatgcgct gagggttact gcgtcgggct tggcgttttt gtgttctttg
     8101 acttcggtaa tgtgtttgcg gttgtaccat ttgccggttt tgtagctgcg gcgttctaag
     8161 gtatagagtt cgccctgtcc ggcgtaatag gattggtcgc cgtaggattg cagtttgatt
     8221 ttgccgtttt ctgcggctat atcggcggca ctcaaaatga tgcggctgtt ctcattggca
     8281 tacggtgcgc taatgctcac accggtttta cccgaaagtt ctgcagcaag catagggggt
     8341 gtcttcagca cgcacgcatc tgggcagtat atagggaatc tgaatattta cttgcataac
     8401 aaatgccgtc tgaaaaattg tgagcttttc agacggcatt gagccgtaaa tcatggaacg
     8461 cgtgcgtgct ggagcacaca ccttacgcat ggattttagg tttcatgcag gctacagctt
     8521 gctgctgcgg cggtataaaa gtatttttag tgatttaccg tttttggtaa ttttgcccga
     8581 cggggttaaa aatacagttg ctactcttgc tgcatgatat tggcaagatt taatttaaat
     8641 gtttgttgaa tcttgtttac atccttagca aaaatatgat agtcactcga agaatagata
     8701 gattttattc tatccaaatt agtttgttga taacattttt tcatttccgc gatgaaaaaa
     8761 ttatcttttc ctctttcatt gagacagtta ttttctaaca gatagattgc ccatgctgta
     8821 aaaataaatg tactatcttc cggatattca gataaataaa tttcgaattc aattatagaa
     8881 tattcagctg cttcaattaa tgttatatct aaatctctga caaatttttc cgttatattt
     8941 ttaaataatt tcttgaattc tatataattt tttatttttt ccatgattct attttcctgt
     9001 aaaaatattt aattgaatat ttggatagcg atttctaaac tctaaaataa cattgctgca
     9061 agattgacag gcctttaatt ctgtaaatag atcaattcta cctgatacat tacgattatt
     9121 tccgagttgc tgtgctatag tttcaagtaa tttatattct ccatctatat ttcttaatgt
     9181 tcctctagga ggagaggcaa tattatgata tttatcaaca gatataggtt taaaaatttt
     9241 tgtttcaggt aacgaaataa atccatgttc ccctttttgg aaagaactaa atgcttccat
     9301 cctttgcggt aatcctttaa catcaatttt tgctatcgca agattcccta tttgtcgata
     9361 ctcttccggc agtttcatgc ggattctctg gctttcagca gttaaagctt gtcttaaagc
     9421 aggagcactg tcattgctca gataacgggt aacaggtgca ggcttggccg atattgcagc
     9481 ctgtgcagat acagttcctg caaccatcaa tgtgccatta acaacctgaa ctgtaccatg
     9541 cagatattct ccattagaga ttgctttcgc tccctgtcca atgttatatc ctcctgctgc
     9601 cacagtagct gctttgacac tgaagccaag cggtccgttt aataagtaaa gtgcaggttg
     9661 tgcggcaaca gctgatttgt aatcaaaaga atcataacga ctcaatgttt gataggcttt
     9721 cgataaggtg ttattaatat ctttggaagg aatagtgaga ttacccattg actggggcgt
     9781 tttcagtatc gaagctgccc aagctgtgtt acctccagag gtttggttaa taaaatatgc
     9841 aagaatattt ttttcttgat tatttaaact acttggattt ttttgatatc ggtcaagcaa
     9901 ttgattagaa tatcgatctt tctcaatcaa atagataaac tgccttgcct cttgttcggt
     9961 caggctgccc caatctaaag ataataggcc tttccctttg gcttttttga tcaatttttc
    10021 tctaagagca tactcttctt gagataatag gttattttca atcgcgactt tagccgcatt
    10081 cgccgctgta ttcacatcgc cgccgaccac accgcttacc gtaccggcaa ccagtttgct
    10141 gtatgccaaa atctgttcgc gttctttagc tgtcaaagtg gcaggatttt tgccgtttgt
    10201 caaagcctcc ccgactatct cgcccacagc cgcaccgatc gcaccatcct gacacttacc
    10261 cttattcgcc gccgctgccg cacagcccgc tatggcatgg gcaatcttgt ggactatgta
    10321 gtgctgatcc aactgtttga ttttactggc tgcttctcca tgcgcggtat tgaccaaagc
    10381 cgcaaggata ttcgcttcca gattgtcttt caggctgccg ccgttgacag cggtattaat
    10441 cagtgcggca ctgcccgcat tggctaggtt gacggtcagg ttgttgatcc actgcttatc
    10501 gctgacattg ttcagtgccg aagcgccgat tttgtcggct acgcctgcgg tagcggcggc
    10561 aaccaccaga tttttcaccg tgctgcttct gcccagctct ttcagggttt tgccgacatc
    10621 gcctttgttg ttgatgaacg atacggaagc ctggctggcc aaagaggcga atgctgcatc
    10681 ggttgcggcg gcggccgcac cgtttaatcc caatacggct ccggttcctg cgcctgaggt
    10741 gaccacggta acggccagtg cgataatcgc tgcaccggct ccggtcaagc cttcctgttt
    10801 atagtcccat ttgtcgtacg ccagttgtac ctggttccag ttgacgtctt tggtgacttg
    10861 gagctgtttc aaatagcaag cgtagggggt gtcttcagca cgcacgcatc tgggcagtat
    10921 atagggaatc tgaatattta cttgcataac aaatgccgtc tgaaaaattg tgagcttttc
    10981 agacggcatt gagccgtaaa tcatggaacg cgtgcgtgct gaagcacaca ccttacgcat
    11041 ggattttagg tttcatgcag gctacagctt gctgctattc atcaaattgc ggccattgaa
    11101 agtctgttgt tttactttca cctctcaaca gtctaatcat atcgcttttg agaaactcaa
    11161 aaaaattttt aatattacca acatagagca tagcttcaca tagtgaacta catgcagatt
    11221 taatgtcttc attgtcaata gcatattgat attccttcat atgctgaaaa aaagaatcaa
    11281 agtcttcttc taattcatca ttccaatcag atgaatagtt agaaagccat tgtaagtcaa
    11341 gaggatcttc actattcaat ttttcagttg tggctttctc ataaagatca aatccttgtt
    11401 taattcctaa ctctcttaaa ctttctttta ctacattaaa atttttcatc tgaatcacct
    11461 tatttaagat tcaattttcg cccttgccct gctaatgtct tagcttaatt ttgagcgagt
    11521 tttaggtttc atgcagacta cagcttactc agcacacacg agtctaaaca gtatacaggg
    11581 aatctaaata tttactttca taacaaatgc cgtctgaaaa aattgagctt ttcagacggc
    11641 atatggccgt aaatcatgga acgcgtatac tgaagcccac accttatgca tgggttttag
    11701 atttcatgca ggctacaact tgctttctat tcatcaagag atggccatga aaaactattc
    11761 tttttatact cagcactcaa taatgttgat atatcagttt ttattgaatc aaatataaga
    11821 gatagattac ctgcgtaaat catagccata aataaagaat tactggcaat tttgaaattt
    11881 ttatcgttta gggctaattg gcatacttcc atataatcta aaaagttttt taaatcctcc
    11941 ttaaattcat tatcccaatt cccgtctgaa gtataatctt taatccattt catattagct
    12001 gtttcatgat taacttcttc tgatactttt ggatctgtca aatcaaaccc ttgataaagc
    12061 cccacattaa tcaagatttt ttttatatca ttcaatgttt ccataaaatt tcctatttta
    12121 agtttaattt acgacctttt gctgccccag ttttcatttg gttaagcgaa ccatccatat
    12181 ttagaacaaa cttaaagttc ccatttttat caaaaacctc taaatgattt ttatgttggc
    12241 catctaaata aaacctatca ccggttttta ataacccttg gtttcttttt accaagaaga
    12301 cagactgccc ttgctgcgtc ggaagcgttg tcttttctga aatttgagcc agctgttttc
    12361 caaaaggatt atttttcatg tatgtactca tattcggtac agcaccttta ttagggatat
    12421 aaggacgatt tttttctaaa acttccttga ccttttgtgc cgcttcccct ttattagcgc
    12481 gattcagctc tgttccgacg acaatatcaa taacggcttt ggcatcgttc caatccaatg
    12541 tttcgtcgaa taaggtggtc aggttgtcgg ctaaattata accttcgtct ttcaacgtct
    12601 gttttaaatc tctaacgttg attttcccgt tttttaatcc ttttctggct accttataaa
    12661 ccacttttgc agcagttaca acagctttaa ccgcattatt ttctaccgcg ttttgtgcgg
    12721 tttgtgcagc agtattgaca tctcctcccg ttacgcctgc aactgtacct gccgcaagtt
    12781 tggcataggc ggtaattttc ttaacttcca gatctaattg ttccggggtc atatcgctaa
    12841 aatcggtatt tttaaccaaa gcctccccga caatctcacc cacagccgca ccgatcgcgc
    12901 cgtcctgaca tttgccctta ttcgccgctg cagccgcaca gcccgctacg gcatgagcga
    12961 ttttgtgggc gacatagtgc tgatccagtc ctttgatctt actcgccgcc tccccatgcg
    13021 cggtattcac caatgccgcc aggatatttg cctccagatt gtctttcagg ctgccgccgt
    13081 taacagcggt gttgatcagc gcggcactgc ccgcattggc caggttaacg ttgaggttgt
    13141 ttacccaagg ggtttcgctc caagtggcaa gggaagaggc accgagtttg ttggatacgc
    13201 ctgccgttgc cgccgctaca accagatttt ttaccgtgcg gcttctgccc agttccttca
    13261 gggttttgcc gacatcgcct ttattgttga tgagcgatac ggaagcctga gaagcgagtg
    13321 aggcaaaggc ggcatcggcc gctgctgcgg ctgcgccgtt taagcctagt gcggctccga
    13381 ctcccgcgcc cgcagtaacc acggtaacag ccagcgcgat aatcgctgca ccggctctgg
    13441 ttaagccttc ctgcttatag tcccatttat cgtaagccag ttgcacctgg ttccagttga
    13501 cgtttttcgc tacttggagc tgtttcagat aggcatactc gggctgtttg gccagctttt
    13561 cgatttcggt tttcagattg cctttgggga tgtcgacaat gtagccgccg ggagcagaga
    13621 gcttgggcac aacggggtcg gtgaagctcg gcaggagccg cggagcttca aacaaggggg
    13681 gcttaacact cccgcgtctg tacagcatac agggaatctg aatatttact tgcataacaa
    13741 atgccgtctg aaaaattgtg agcttttcag acggcattga gccgtaaatc atggaacgca
    13801 tgcgtgctgg cgcacacacg ctacacgtgg attttagggt tcatgcaggc tacggcttgt
    13861 ttatttaaat tcatcacgtt tcattggaat ggttaaaggg gtttttaata aaaattgtga
    13921 attatcgtta taaaaagcac atccacattg agtacagaga tacaccgttt catgcgtatt
    13981 ttgttcttct aatttttgca tttcacccca ttcgcagtaa ggacagactc tttcaatatg
    14041 ttcaataaat aaatattcta aaatatctct aatatattgt acaaagtatt gaaatttaaa
    14101 atttcctgat gatattttac gataaatatt gtttttgaga taaataattt cctctctatt
    14161 caatatagat ttttcaaccg gatagtcatc gggattcatt tcgatatatt tactaaagtc
    14221 ataacctaaa attttaaatt tatcaatgac aatatcgttt gataaccatg aaatcaaaac
    14281 tttcaataaa tcaaatgagg tagagttatg atttaaatgg cttaaagcat catttaattc
    14341 taaaatatat tttttttcaa agttactcat atttagttag tttcctttta ctgggtatgt
    14401 agtaatcaaa tttcctgact tatctgtaaa tactttaatt gtagttgtgg gttgtccacc
    14461 ttctttaata gaagtagtac caataacttt tcctacatcg acagtccgca tatattggcc
    14521 atcaggagtc atcgatacgg gagaagaaac tactttatta ctttgaagta taaccttcaa
    14581 ttcatttggg gagatggtaa aaactgaacg gttattcgca ataggcctat ggaagtgccc
    14641 ctcaagaaca tgctcaaagc cagcagatac cggttgtccc gtttgtctca ttggtgtata
    14701 tcgagtagta ctattcgcta tattaattcg tgtatttaca gcacctatat tttttgattc
    14761 ttgtagtaag ccatcaagtt ctttaactgt ctgggttggt atagcctgct tagccgcctt
    14821 cgctttcgac aacgctccta ctggagcttc ccaagcgttg cctaccgtta ccgcacccgt
    14881 ggcgattccc gcgcccgctt cagcagcctg cgtaaccatg acagtacaac cagaaggatt
    14941 agccatacag gtgctgatag ctgctttacc agctgtaccg atcagcggag ctgtccaacc
    15001 tgcagcataa accccatagc tggtaatcac aatcgggcct gtgatgccat tacggatatt
    15061 gcttatccaa atggcagcat ccttatcctg cggattagtc atcgcacctg ccgcatgtgc
    15121 aggcataata ctttggatga ttttttccag tgcggttttg tcgggcttct gcggttgatg
    15181 ctttttcgca ttggtagggg tgctgtcaaa attcaaagca ttattcacca cagctacctc
    15241 agccgcattc gccgcagtat tcacatcgcc gccgttgagt gccgccacgc tgcccgcaat
    15301 aatcttcgag taactgataa ctttatgctt ttctgcatcg ctgagtgtag cagggtttct
    15361 gccgccaagt atggattcag ctacgatttc cccaactgct gcgccaattg ccccatcttt
    15421 acattttccc tgtaccagtc cgctaacaca acccgccaaa gcgtgggcga actgtttggc
    15481 aacataatcg tcgctgaagg ttgttttgat tttgctggcg gcttcccctt ggaagctatt
    15541 aaccaatgct cccaatgcgg cattgcctaa gttgtctttc aagctgccgc cgttaacggc
    15601 ggtattgata ccagctgaga tacctgcatt actgagattg gtagccagtc tgcctccaag
    15661 gttggcaata gtttgattgc ccgtactgct gaacagttcg gttcttacct tgctgttcaa
    15721 ttgggcaata tctgcgccca tctgatttaa tgcacccgcc gtcagggcag aagtgacaat
    15781 ctgcttgacc gtatcactgg tgccgagatc tttcaacgct ttgccgacat cacctttatt
    15841 attgatgatg gatacagctg cttggctata caaggaggct aaagcagcgg tttgcatggc
    15901 agtcgctgta gaaacggtag tagctgctgc tgtcgttgtg gcggctgttc cggcagctgc
    15961 ggctgtacta cttcctgaag cggctacacc gcccgctgcg gttgcgccgt atccataagt
    16021 cagtgcggtt acgattatgg taacaatcgc tgcaccggct ctggttaagc cttcctgctt
    16081 atagtcccat ttatcgtaag ccagttgcac ctggttccag ttgacgtttt tcgctacttg
    16141 gagctgtttc agataggcat actcgggctg tttggccagc ttttcgattt cggttttcaa
    16201 attgcctttc ggaatgtcga cgatatagcc accgggggcg gtcagtttgg gcggagtagg
    16261 gctttcgaag ctgggcagtt tcagcgtttc gatagtgctg ccgcgtccgg cctgtttctg
    16321 ccatacggtt gagttggttt ctaatttttc ttccgactgg atacggttca caatgccttt
    16381 gaggataatt ttcgcatcgg cacgggcttt ttcgcctaca cctgcctgaa tgtccgcacc
    16441 ggccagcgtg gttttgaatt cggtaccttc gagcacggta tcccagcctg aacgggtggc
    16501 tgcagtttgg gcgacgacgc ggacaggcaa tttggtttcg ttcagttcgt ttttactgta
    16561 attgctcttg cctaccttga tgccgataaa gcggcggctt ttttggacat ccaactcgtg
    16621 cttgtggatg ccttcttctg ccagcagttg cagctcttca cccgcaacca gggtaacttt
    16681 acctgcaggg gcattgaagc gggtggtatt agcttcgatg ttgccgcctg cctgaagcgt
    16741 tatgccgttg gcggtcagct cgacgggggc tggcataatc aggtggtcgc gggtgctggt
    16801 aaacttggtt tttctgatga ttttgccgct tttacctttg gtttttaaga aggtataggc
    16861 atcgttttgt ccagcctcca gtacaatatc actatgggct ttgatgtcta tgctgcctga
    16921 gggagctttg atttcggatg caccgataat aatacgtgca tcatcgagtg ccgcagctgc
    16981 atgaatactt acccctgtac gtccggtcaa acgtgaaggc ttgttcagag cagctttgtc
    17041 gtagtgactc ttgtaggtgg gcttgccaat ttcatattgg tcggttatgc cgtcaatcag
    17101 aatagcagcc gcctctgaat ctgctgcctt tggcaatacg cctgcggcgt gaaggttcag
    17161 ttttttggaa gcggtaatat cggaaccgct gatttcgatg ccttgtgcgg aaatcaagtc
    17221 aatattttgt gcagaaagct tggcttgcag gtattctttg cctttgggtt ttttaccttt
    17281 aacttccttg ttgatggctt gaatatagaa agcgagacgg tcgcgttctt cttgcagggt
    17341 tggaatcagc ttgcttttag gcgagctttt tttcaactgc gcaatctgct gttccaattc
    17401 tttggatttt tggttgagtt cagccgcttt ttgtgtagga aaataattgc tgaatgagtt
    17461 gtttacggct tcgatattca acttgccttt ggtggtggcg acaaccaagt ttttaccggc
    17521 tgtaatttta gaacctctta aatctgtttc tcctgtaacc agacggatat tgccttttgc
    17581 ttccaatgag gaaacttgag cactaggcgc acctgcattt cctccttttg cagacaacag
    17641 caattttccg cctgttttga tgctcaggtc ggtatgcgca ctgatgcggt tggcaggttc
    17701 gatggttaat gtgccgctac ctgcttcaat attcagccgt ccggccaatg gttttaattc
    17761 ggcattatct tccaaagttt tggtcgaaac ggtactccaa ttgatgttgc cgcgcttgac
    17821 tagggcggta ccggcagtaa ggttgattgc acccgctctc agcgtggtgt tgtcggcaat
    17881 ttgggaatag cgcgcattga gtgccaatac accgttagcc accagcttgt tggcagaagg
    17941 cagtttgtcg ttttgccaaa tctggctgcc ggtaatgctt agatgacggt gtgcgtaggc
    18001 atctacttgg ttgagcgtta cccgctcgtg ttgtgcatta agatgcgtat tatgggtaga
    18061 ctccagcttg gtattttcta tgctcaatgc ccggtcggaa tgaatgttca atgccccgct
    18121 tttggcatgg acgttaaggt tttttaagtc ggcattacca ccgttgtttt tgatgctgat
    18181 gtgttttccg ttgattgaat tgcgttgttt tccgtcacca agctgaatac cgttgccggc
    18241 aaccaacgta atatctcctg aagatgaagt gatattggta ttgtctgctt tcagacggcc
    18301 tttaccaacc gatcctgcat tgacatcggc cttggctgtc agggtattgt gaccggtaaa
    18361 gtcggcatta ccgttggcca ataaggatac atgaccgtct gcagaaacag catgaaggcc
    18421 gtctgaaacg atattgccct gcaatgcggt ggtttcgaga gccttggctg cgttcagctt
    18481 ggtattgcga agctgaatat tgcctttggc tgcctgaatg tgcagattac ccgagttggt
    18541 acgcagattg gtattggtaa cattcagcga gcctgcctcc acacccatgt cttttgaggc
    18601 agtgagggtt ttactggtgc cggtaatatg ggcagcgtta tccgatttca aatggatgct
    18661 ggcggcagac aaatctttat caacattcaa attcagatct ttacctgtat gaacatacag
    18721 attgccggga gtattcagat tggtagtttt ggcagtaatg ttatcgtctg ccagtaaagc
    18781 aagctgcttg ccgcccttga tactgcctcc gtttaagcgg atatcggagg taactgttga
    18841 agcttccaaa gaaagcggtt tgcctgcttc gatgtgtgcg gtgtctttgg catctattac
    18901 ggcggaactg ctgatggtgc cgttggataa tacggtaaca tctgccccgg taatgcgtgt
    18961 gttattgcct aattcggcgt tgcctttgct ggaactgtat acggtagtgc cagtctgaat
    19021 actggcctcc ttgatgacgg tacggccgcc ggtcgacaga ttggtcgaac ctttagcatt
    19081 gttcacattt gctttgcttt cgataaccaa attacggccg gcatttaata ccgtagcagc
    19141 agggcggttg ccgttgtttt gaacgaccgc tccgttacgc aaaccgatgt cctccccggt
    19201 atcaataacc aataagcctt tgctctcgat ccgaccatca ttggagataa atgtgcctgc
    19261 cgctcctttt tcggtggttt cgatggagag ataagtcggt gaagcttcgg tgccgtcggc
    19321 agtggtggcg atgcggccgc tgttttcaat gcggcctgac gaagtcacaa tcaattgctt
    19381 ggccgcttcg agtgtgccgg catttttgac gcctacgcct ttttcattgg caatcagtgt
    19441 gatgctgtcg gcgtacatac cgcccagtgc ggcagtatca agggcaatag tcggtttcgt
    19501 acccgctgcc gtacctgcac tgatttcgcc gctggcgtaa tctactttct gaggaccggt
    19561 agaaaccgcc aggtttttac cctgtaattt cccctgcaaa gcaactgcac gagcaagtac
    19621 cccggtgtag tcggcttcgc ctttatcatt ccaacctgct gctcctacgg tcaatgtgcc
    19681 ttgacgcaca tcaaatcctg tcagtgcacc gtctttgccg atttggggcg caccggtagt
    19741 taagatgccc cgaccgacat ttttaaagcc gccgccatta acggtaatgc cgttggggtt
    19801 ggcaataatc acgtcggcct tttgaccgcc tacggtaacg atgccgttga gtttgctagc
    19861 cgtaccgcgt acctcgttca aaatcaattg cgcactgcct ttgaccacaa acggattatt
    19921 gttacggtcg ttgtttaaca ctgccccttt gttgtcaaca tcaaactgcg tatagcggtt
    19981 gtggctcaat ccgcgtccat tcggagtttg gatattcacc aagggggcac cagtgttggt
    20041 tttaaggata acgacctgct ggtttttagg tgctgatttg tcggtggtaa tttgggcatg
    20101 ggcaggcaat accatactca gggaaaccaa agagcagacc aaagttttaa gggtggtttt
    20161 gagtttgccg caaaggtcgc ctgaagtttt cagtgaaaca gaaaccgaac tgcctgcctg
    20221 tttacctttg ccctggctgt tggcagtttc ggctactgca accatggtgc tgtgcttttt
    20281 actaaagata atgcgatgta aacctttatt catgtctatt ccattttgaa gatgaacgta
    20341 ctgcgcgcca agtacgtagg taaagtttga cggtctgagg ataaggaaag accgtctaaa
    20401 tatcagtaaa aaaattcaga ggttagaaac tgtaattcaa gttgaagccg taaacggtgt
    20461 tggccgtctg aaaaccttcg ggcttgtgca gcggcttgcc ggcaaataag tcataagcaa
    20521 atataccgcc tactttatgg ctgcctctga agccggccac tgcacccgcc agccgcttgc
    20581 ccgaggcata ttgggcactt tcgcccgata cccgaccata gtccgcgccg agatagaatt
    20641 gatggcgcgg atggaaatgc cagcccaaag tgttctgcca gtagaaacca cgctctccgg
    20701 aaaggctctg ttctccgtca aatcctcgaa cggtgtagcg gctgccgata gacaacttgt
    20761 cttgggcaac caaaggcgtt ttgttccatt gagcttgaat ggcggttgcg taggaaaact
    20821 gctgtttgcc caacataaac ggggccgctg catccaatcc ggcggttatg attttcatac
    20881 gggatgtgcc tggaatagta ccgccgccgt tttcttccgg tgcgggcata ctttggcgca
    20941 taccggtacc ccgtttgtaa gataacttgc cgtccagttg ccagcggttg aggtaagcac
    21001 ggtggcgcaa ttcggcttcc cagcctgcag agcggcggcg ttgcacttcg atttcggcat
    21061 cgtcgatgta tttataggtt tggcgtgtcc ataatttcat tccgactgaa gttttatgaa
    21121 acctgttacg ccaaagcatg cgctcggcgg ccaggctgct ctgatattgt ttgccgttgt
    21181 aatcgtaatt gacggaatag ccttcggttg cttcgtggta acgatgtcca ttgtgattaa
    21241 aagaaaacag ccattttttt acgggcaccg aataatgcac gctgtaactt ctggatccgc
    21301 tttcagtttc cgtaccggtg gcatcagtca agtccgtttt gtgcaccaaa ccgcgtccat
    21361 atgaaacata aaacaaatcg cttaagccca aagggttatc gaacgataaa gcgacatttc
    21421 cttgatattt gccggtcgtt ttgccgcccg catcatctat accgatactg aaccgtatgg
    21481 gtttattctg ctgccatttg atctgtaaat cgcttttgcc ttcttcttcg gacggtataa
    21541 tctgaatatc tgttttaaca ctcggcaaac gacgcaggtt ttccaagccc tgctctacat
    21601 cgcgaagatt gagaattttg ttcctatata agggaaattt gttattgaat gcactaatac
    21661 tgccctcggc agacttccca tcccgttttt cttcatagcg gatatcccct atttcgcctg
    21721 ctgatacccg taatttcaga attcccgaat ccatattctg tggttggata atagcttggg
    21781 aagtgaggta gccacgcacg atcagtatct gttgcgcggc tttttgtagc ctgctcaaat
    21841 tattggaacc taaacacatc ccagttttaa aagctgtttc tttcatgagc acagaaggaa
    21901 gaaaagaaaa tttgcgcacc gtcttatcat ctaaactaat gtaatttacc cgagtacacg
    21961 gtgtttcatc ttcactcagg acataattgt tcttctccaa tggttgctcg aaacggacat
    22021 ttgcatcagt taacaattca gcatctatgt gctgctgacg ctgcatggaa cggataagtt
    22081 ctgcatcgtt ttcatcggca gctaaggttt taaggggtat gacagccagg ataaccaaca
    22141 gacatggagc aggaaaaaat ttcatgacat caatattatt ttagcaatat ttactatttt
    22201 gtcataaatt taaaagtatt tacagttata gaatgagacc tttgcaaaat tccccaaaat
    22261 tcccaccaag acatttaggg gattttgggg aattttgcaa aggtctcgga cagtattttg
    22321 aacgcagtgc gcgtaaattc gtatggaaac catgaaatcc cgccacagcc gccagacatg
    22381 ccaagccgca ttctgatatt tctgtttgca ggataacagg cagctttttc tttaagccca
    22441 aagacaggtt ttgcagatgg ggcatagatt tcctttttga aaaataggga ttaggaagtt
    22501 ggatgtattt tagaaaggcc gcctgaaaag gtttcagacg accttttgcg actagctgct
    22561 attttattta aagcttttct ctaacaaacg agctaatatt tttcctgtaa taaaacagat
    22621 aaaaaacagc atccaatacg tcagattgga aaaatcggtc gtatagagaa tcaacatata
    22681 aagaagcagc atgatgccga gtgcgatgaa ttgataatgt ttggcaaaca tcatgacctc
    22741 ctcaactatt aaggcaaacc gcctgaatat tctcgttcaa tcgtttcggc aatttcccta
    22801 taacgtcgat accatgacca gtcgaaattt tcaatggcat ggctcgcaaa cgtaccaaat
    22861 tcaggcatcc ctatgcggct acctgctaaa gctccgattg tagctcccca accaggcgga
    22921 ttactgaacg tattgtttgg caatcctaaa gatttatcag gatttcccgt atcgttaagc
    22981 cctgcagatt cgcaaatttc gcaataatat gagctttgtt gcgcattacc tgagcctccg
    23041 acccaagtca tttcatgaac acatatagtg gattaaattt aaatcaggac aaggcgacga
    23101 agccgcagac agtacaaatg gtacggcaag gcgaggcaac gctgtactgg tttaaattta
    23161 atccactata aaactctcat tttgaaactc cttgtatcgt taatcaaaca atcaaaaggg
    23221 cagatgccct atccttgctt ttacaaacgg agtgcctgta aaaggggatg gtttcaggca
    23281 gttttgaagt ttgtgttttt atatattgtc ttctggtcgt ctgaaaaggt ttcagacaac
    23341 ttctttatct ttacagcctc aagtcttaca gtttgcccga catactataa atcagctcca
    23401 atacccattc gtacaatcac cgtttctcgt gtaggatgtc tgcttccaac gtcatgccga
    23461 tttgcagcgg tttttcctca ccgtatgcag tgatggttga tttgtcgggt tttattttca
    23521 caagataaac aggttcgttg ctcttcgcca aatcggagga taccatgccc aatcccgaca
    23581 attcctgtct gcccagtgcc gtttttgcta ctgatcttcc gcaagtctaa tgcgcctttt
    23641 ctgaccgtct atctgttgcg aaatatggag tttctggttt tccaaacgtt cgacagttgc
    23701 tttaaggctg cgcgtttcat tcccgtgtat cagcttcaga cgacccagtt cctgttctgc
    23761 caacgttttc ttcaaaactg cctccgtttt caactgctgc tgcacgctac ctcctgcgcc
    23821 gaaacgtgag gtcgaaagcg caaatagctt gtcgccagcc ttaacctttt ctccatcttc
    23881 cacgaatttc gctgtaattg tccccgtatc cggtgcatac accctgatta cgcctgatgc
    23941 aggtaaaatt tgtccctccg ctgttgtctt tcgcgtatag ttaccaaata tcaaaaacag
    24001 gataatcaat aacgcagata tcgatgcaaa tgtcgtccat agggaaaatg acaacggtcg
    24061 tgtcagaatc actttacccg tcaggctggt ttggcgggca acggcgactt cgggacggaa
    24121 gaagggttgc ttgggtctat tcataaaatt gaagttaaga aagtttcaga cgacccctag
    24181 agatcgtctg aacaatgaga aatatcagca gtaatctgta ccgtcagtgt agccgtttcc
    24241 tgatttatct gcttttgttg cgggagcagt taatccatgt tcaatctcaa agattggtct
    24301 tccgttataa ggaggtgcat taacggcatc atttacccaa tcacgagtca cattgtatac
    24361 accatttgca ccagcagcac cgtaagcatt tttcggcaga taataaactg ccgctgcggc
    24421 agcaggtatt gcaaccaaat ccccccatgt gggacctcct ttggttgtgg cagcattagc
    24481 tacatttcca gctatattgt ctgttacagg acctccccct gaaaccagct tcaattcatg
    24541 aagttgaagt tcaatcattt ttatactcct tttcttggtt ggtattccta aaaattcggc
    24601 taacaaaaac atatggcaga tatattgaaa aaaaattcaa agtaccctga ataaaattca
    24661 aattccaact atatttgtta atgtagtcga gaagaaacat atctgataaa aaatatagca
    24721 cttgataaca agctattact aatattacga aaaatgtaaa ttgcttccag tttttcatag
    24781 aatccctcac aaaatttcca gaaaatctaa ctctatcaac tgataaatca acttcctaac
    24841 ttcttcatat tttccctgat tgaagttaac cagtagattt ttcaacaata acggttcatt
    24901 cttaccgatg tgttctaaaa cttttttccc caactcatct acgcttatct tcatcccatt
    24961 cccaatcaaa tatccctttt ccaacgtatc caaattattg gcatttaatc tcaacctgac
    25021 gtcgtctgaa agcggagtag cgttgggatt cgcgaactgt tcgagatgaa aagcggtatc
    25081 ggtacgttct ttgccgagaa agtcttcact gaaggcttca taattgacgg ggtcggcaat
    25141 catggcagca atttgtgcgg cagtatcgtt gatacgcgtc ctatcttgct cccagtctga
    25201 gaaactgtgg cgcagacttt ctatcgtggg gaatttcttc attagccact cgaggtaatt
    25261 atagccgttg ggcggaaagg taccgacagc gaggtggaag gtttcacagc cgagcgggat
    25321 aggtctgtgc caccaaccgc gcgggatgta gaggacatca cctgcttcaa ggataatatc
    25381 catatcgata tgttcaggaa tggaaatatc agtatcttta gtctgttgca tatacaatgg
    25441 catagggaaa tcaggggcag taagttgcca acgtttcttg ccgaaaagct ggatggcata
    25501 cacatctcgg atgtcccaat ggtttttata agattcgtcg ctgccaaaag caagataccc
    25561 actaacaatg gtatgtgcgc cggcaaggcg aggcaacgct gtactggttt aaatttaatc
    25621 cactataata tggttataga gcaacgacgc gccattcttc atatgttcgt agataacgga
    25681 tttaataaaa cggtagcgag ttttgcccaa atcgtcgaaa ctttcgacgt attcttcttt
    25741 aggaacaatt gcaccttttt tacgcagatg aaacagcggt gcggttgggt ctgctcgttg
    25801 gtatatatcg ttgatatctt tccaagatgc ggcttcgaga ttccgaaccg ctcctttaaa
    25861 gagcttgggc ttttgataca gataagtctg tcggaacgtt ttaggactaa tgccgaagtc
    25921 gagatggatg ctcattactt ccccttactc agaaaatatt taaaatttat aatgttacat
    25981 atatttacaa atattaaagt tttttttgtg tgtgcgtcaa ggaattgttg ccaattttgt
    26041 tatagtggat taacaaaaat caggaaaagg cgacgaaacc gcagacagta caaatagtac
    26101 ggcaaggcga ggcaacgctg tactggttta aatttaatcc actataaaaa tttgttttgt
    26161 ttccaaacga aaaaaagcgg tttgtttttt gttgttaaca tttttattgt aattaaataa
    26221 aaggtcgtct gaaaacggtt ttcagacgac cttttgctat aatcgggctt catcgccccg
    26281 ttcggtttgg aaccttatga aaaccctcgt cctcctcctg ctttccttct ccacgaccac
    26341 cgctttcgcc gcatacggtt tgggtttggg gcaggcaccg aaatatcctg ccgactttcg
    26401 cgcctacggt tatgtttatt ccggacggca gggctgggtt ttaaaaacag aggcggatgc
    26461 cattaaatta gacacgcttt tcaaacgctt tgtgtaccgt ccttccgccg ccaatcaaaa
    26521 ccccgtcgga cagcgttcgg acggcatacc cgccaaccac acaaaggaaa aaccatgagt
    26581 aaaaaaatca aagtcggcat tgtcggcgcg acgggctaca ccggcgtgga actgctgcgc
    26641 ctgcttgccg cccatcccga tgtcgaagtc gccgccgtaa ccagccgcag cgaagcggga
    26701 accgcagttg ccgattactt tccaagtctg cgcggcgtgt acggcctcgc cttccaaacg
    26761 cccgacgagg caggtttgga acaatgcgac atcgtcttct tcgccacgcc caacggcatc
    26821 gctatgaaag acgcgctgcg ccttttggaa cagggcgtgc gcgtcatcga cctttccgcc
    26881 gacttccgca tacgggacat tccgacctgg gaacactggt acggcatgac ccacgccgcc
    26941 cccggcctcg tttcccaagc cgtgtacgga ttgagcgaac tcaaccgcga agccgtcgca
    27001 caggcgcgcc tcgtcgccaa ccccggctgc tacccgacct gcgtatccct accgctcgtg
    27061 ccgctgttgc ggcaatgccg tctgaagccc ggtatgccgc tgattgccga ctgcaaatcc
    27121 ggcgtgtccg gcgcgggcag gaaaggcaat gtcggttcgc tgttgtgcga agccggcgac
    27181 aacttcaaag cctacggcat agccggacac cgccacctgc ccgaaatcag gcagaccatc
    27241 gccgggcttc aggacggcat cgccgaagga ttcgtgttca cgccgcacct cgcgccgatg
    27301 atacgcggta tgcacgccac cgtttacctc cacctttcaa acggcatctg ccccgaaacc
    27361 gtcctgcgcg actattaccg cgacagcctg ttcgtggaca tcctgcccgc cggttcgaca
    27421 ccagaaaccc gcagcgtgcg cggcgcaaac ctctgccgca tcagcatcca acaggcggcg
    27481 caatccgatg tgtgggtcgt cctttccgtc atcgacaacc tcgtcaaagg cgcggcgggt
    27541 caggcggtgc aaaatatgaa catcatgttc ggactgaagg aaacacacgg cttgggcgca
    27601 atccccctgc tcccctgaag cgcaaacggc aaaccgcagg catcgtgcct gcggtttttt
    27661 gatgccgtct gaaagcggcg gttttttttg gttcggacgg cttttggctc attattccgc
    27721 gaaaacaaaa atctaaaata ccgtcattcc cgcaaaagcg ggaatctata gtggattaaa
    27781 tttaaaccag tacagcgttg cctcgccttg ccgtactatc tgtactgtct gcggcttcgt
    27841 cgccttgtcc tgatttaaat ttaatccact atagtttatc cggcttcagc gatttccgac
    27901 acatttccgc acgcttcaat ttcgtcattt ctccggtttc agtcattgcc gataacaccg
    27961 tggtttttca tttctagatt cccgcctgcg cgggaatgac ggcggagggc ttgccgtttt
    28021 tcccggtaaa tacctgcaat ttaaaatccc atcattgccg tgaaaacaaa ccaaaaacct
    28081 aaaatcccgt cattgccgcg aaaacaaacc aaaaacctaa aatcccgtca ttcccgcaaa
    28141 agcgggaatc tagtttatcc ggcttcagcg atttccgaca catttccaca cgcttcgatt
    28201 ccgtcatttc tccggtttca atcatttccg ataacaccgt ggtttttcat ttctagattc
    28261 ccgcctgcgc gggaatgacg gcggagagct tgccgttttt cccggtaaat acctgcaatt
    28321 taaaatccca tcattgccgt gaaaacaaac caaaaaccta aaatcccatc attgccgcga
    28381 aaacaaacca aaaacctaaa atcccgtcat tcccgcaaaa gcgggaatct agtttatccg
    28441 gcttcagcaa tttccgacac atttccacac gcttcgattc cgtcatttct ccggtttcag
    28501 tcattgccga taacaccgtg gttttttatt tctagattcc cgcctgcgcg ggaatgacgg
    28561 cggagggctt gccgtttttc ccggtaaata cctgcaattt aaaatcccat cattgccgtg
    28621 aaaacaaacc aaaaacctaa aatcccatca ttgccgcgaa aacaaaccaa aaacctaaaa
    28681 tcccatcatt gccgcgaaaa caaaccaaaa acctaaaatc ccgtcattcc cgcaaaagcg
    28741 ggaatctagt ttatccggct tcagcgattt ccgacacatt tccgcacgct tcaatttcgt
    28801 catttctccg gtttcagtca ttgccgataa caccgtggtt ttttatttct agattcccgc
    28861 ctgcgcggga atgacggcgg agggcttgcc gttttcccga taagtctctg cggcttccca
    28921 ttatcgggtt tccgtccact tgggaataac gggatttgaa atcatgaaaa tgtgtcaaaa
    28981 ataatgcaaa atcagatttc cgttacactt tttttctaat atttcagacg gcattttgct
    29041 cacacgccca aatacccttc cctgccggaa agccaccttg ccaaatgcgc ttcgacgatt
    29101 tcggggtttt gctcaatcag catcggggcg atttcgcgca cttgttccaa aaggtgtaag
    29161 tcttcttcaa gcttggcgaa gcgcagcata ggcacgccgc tttggcgcgc gccgagaaat
    29221 tcgccggggc cgcggatatt gaggtcttgg cgggcgattt caaagccgtc ggtgtgttcg
    29281 taaatcactt tcaaccgcgc tttggcgagt tcgcccaagg gttcggcaaa caggaggacg
    29341 cacacgcttt ctgccgcgcc gcgcccgacc cgaccgcgta attggtgcag ctgcgccaag
    29401 cccatgcgct cggcgtgttc gatgaccatc agggcggcat tgggcacatc tacgccgact
    29461 tcgatgacgg tggtggcgac caagacgttc agcccccccg aagaaaaccg cgccatcact
    29521 tcggcctttt cggcggcctt catgcgcccg tgtaccagtc cgatattgag ttcgggcaat
    29581 gccgtctgaa gccgggcgag ggtttcggcg gcggtttgca gttgcagggg ttcgctttct
    29641 tcaatcaatg ggcagaccca atacgcctgc cgcccttttc ggcaagtgcc gaggacgaag
    29701 ccttcgactt cggcgcggcg gacgttgttg acgaggcgcg ttttaatcgg tgtgcgcccg
    29761 ggcggcaatt cgtcgatgac ggacacgtcc aaatcggcga aaaaactcat cgcaagcgtg
    29821 cgcgggatgg gcgtggcgga catcatcagc tgatggactt cgcgcccttt gtttttgagg
    29881 gcgaggcgtt gggcaacgcc gaaacggtgc tgttcgtcca caatggtcaa gcccaaattg
    29941 tgaaacgcca cgccgtctga aaacagggcg tgcgtgccga cggcgatttt gacgctgccg
    30001 tcggcgagtt tggctttggc ttcgtctttg gcttttttac gcaaactgcc aaaaaggcgg
    30061 acaacttcaa tgcccaaagg ttcgagccat tgtttaaatt taataaaatg ttgttcggca
    30121 aggatttcag tgggcgccat tacagccacc tgcgcaccgg attcgatggc ggtcagcgcg
    30181 gacaaggcgg cgacaatggt tttgccgctg ccgacatcgc cctgcagcag gcggtgcatc
    30241 gggtaggttt gcgccatatc gcggcagatt tcggaaacaa ctttttcttg cgcatcggtc
    30301 agggcaaacg gcagggcttg gcgcagggct tgggtcaatg tgccgtcgcc gcccaatgcc
    30361 gccgccgtgc cgccgatacg cttctgtcgc gccaagcgca tcgaaagctg ttgcgccaaa
    30421 agttcatcga atttgagccg ttgccatgca ggcagcgtgc cgtctgaaag ctgatgaatc
    30481 gtgaaactcg gcggcggcga atgcaaaaga cgcaggcttt cggcgaggtg tggcagcttc
    30541 agacggcaca gcagggcatc gggcagcgtg tcgtgcagcg gcgtaacgtc caacgccgtc
    30601 tgaataatac ggcgcaaagt gggctggttc aaaccgttta cggtcgggta aaccggcgtg
    30661 aggctttccg ccaaaccgcc gccctcggca tcgcggattt tgggatgaat catctcgtcg
    30721 ccgtaaaagc cgtgtttgat ttcgcccacg gcgcggatgc gtttgccgac cgccgtctgt
    30781 ttctgatggc tggcgtaaaa gtggatgaag cgcagaaaaa ggacgctgcc ggaaccgtcg
    30841 gcgatttgga caatcaactg cttgcgcggt ttgaacgtta cttcctgatg gataacctcg
    30901 ccctcgacct ggcacggcac gccaatcggc gcgtccttaa tcggcataat gtgcgtctcg
    30961 tcctcgtaac gcagcggcag gtgcaacacc aaatcccacg cggtatggag gttgagtttg
    31021 tcgagcttct tggcggaaac atcggtgatt ttgagctgtt tctgggtttc gggcgacatc
    31081 ataggcagat tcctttggac gcgcctattt tatccgaaaa caaaaatgcc gtctgaaacg
    31141 gattcagacg gcatcgacag gcaggaatca agccccggcg gcttcggctt cctgctgttg
    31201 ttggtaaatc gcctcaaaat taatcggcgc gagcaggacg ggcgggaagc cggcgcgcgt
    31261 taccgtaccg gaaaccgctt cgcgcgcgta agggaagagg atgttcggac acgccacgcc
    31321 gagcagcagg tcggcatctt cttcggggat gttttccaaa cggaagatgc cgctttgggt
    31381 tacttcgttc aaaaacatcg tgcgctcgtt atccagcttg gcggtaacgg ttacggtaac
    31441 gtccacgttg tagtagccgt cttccagctt ttggctgccg gtggaaacgc gcatatccac
    31501 ttcgggctcg ccctgttcca aaaagatttg cggcgcgtgc ggcacttcca aagacaagtc
    31561 tttgacatac agtcgctcga tgctgaatac gggttgcagt tcttcgctca ttttgttttc
    31621 ctagttgggg gttaagggtt cagcagtccg tccagcccgc cttcctgctg gaggcggtag
    31681 aggtcggtaa atccgccgac gtgcgtttcg ccgatgaaaa tctgcggcac gctgcgctgt
    31741 cccgaaagct gctgcatttc ggcaaaggct tcggggcttg catcgacacg gatttcgtcg
    31801 atatgtccga cacctgccgc gtgcagcagc cttttcgcca tcgcgcagta gggacaaaac
    31861 ggacctgtgt acatggtaac ggtctgcata ttgggtttcc gaaagttttg caatgataat
    31921 caatataggg gcatttcccc tgtttggcaa gtgcggaaca gatgcacgtt caaacggcat
    31981 gtgcggaatg tgtcaaagtt tcttttttaa agtatgatag acattgtgaa aaatattttt
    32041 gcacccgcgc tgcgcggcgg aacggatgca aaatattttt attacatttt caggaaaaac
    32101 catgttgtca ggactcccca tccccaaaga catcgcgcgc ccgcccgaaa cgatattggt
    32161 caacatcacg ccgcaggaaa cgcgcgtagc ggtgttggag gaaaacaata tctgcgagct
    32221 gcacatcgag cgcaacagcg aacacagcct ggtcggcaat atctatttgg gcgtggtgcg
    32281 ccgcgtgctg cccgggatgc agagcgcgtt tatcgacatc ggcttggaac gcgcggcgtt
    32341 tttacacatc gtcgatgtcc tcgaacaacg ccgcaacccc gaagaaaccc agcgcatcga
    32401 acatatgctg tttgaagggc agtctgtttt ggtgcaggtc atcaaagacc cgatcaacac
    32461 caaaggcgcg cggctttcca cccaaatctc gctggcgggg cgtttcctcg tccatcttcc
    32521 gcaagaagac cacatcggcg tgtcccaacg catcgaagac gatgccgaac gcagcagcct
    32581 gcgcgaacgc ctcgacaagc tcctgccgga aaatgcctgc cggggctaca tcatccgcac
    32641 caacgccgaa aacgccaccg acgaacagct ccagtccgac atcgactacc tgaccaaagt
    32701 gtgggaacac atccaagaac aggcgaaaat ccggccgccg gaaaccctgc tttatcagga
    32761 tttgcccttg agtttgcgcg tattgcgcga tatggtcggc tgcgacacgc aaaaaatcct
    32821 cgtcgattcc accgtaaacc acgggcgcat gacgcgtttt gccgaacaat acgtccacgg
    32881 cgcattgggc aggatagaat tgttcaaagg cgaacgcccg ctgtttgaaa cccacaacgt
    32941 cgaacaggaa atcagccgcg ccctgcaacc gcgcgtcaac ctcaacttcg gcagctacct
    33001 gattatcgaa tccaccgaag ccatgaccac gatagacgtg aacaccggcg gcttcgtcgg
    33061 cgcacgcaac ttcgacgaaa ccatcttccg caccaacctc gaagcctgcc acaccatcgc
    33121 ccgcgaattg aggctacgca acctcggcgg catcatcatc atcgacttca tcgatatggc
    33181 gcaggaaagc caccgcgaag ccgtgttgca ggagcttgcc aaagccctcg ccttcgaccg
    33241 tacccgcgtt accctgcacg gttttaccag cctggggctg gtcgagctga cgcgcaaacg
    33301 ctcgcgcgaa aacttaaacc aagtcctctg cgaaccctgc ccttcctgcc aaggcagagg
    33361 ccgtctgaaa acgccgcaaa ccgtgtgcta cgaaatccag cgcgaaatcg tccgcgaagc
    33421 gcgccgttac gatgccgaaa gtttccgcat cctcgccgcc cccaacgtca tcgatttgtt
    33481 tttggacgaa gaatcgcaat ccttggcaat gctgatagat ttcatcggca aaccgatttc
    33541 tctggcggtc gaaaccgctt acacgcagga acaatacgat attgtgctga tgtaaaaaat
    33601 gccgtctgaa gccttcagac ggcatctgtc tatttcaggg tttccttgtc caacaacgcc
    33661 cgtatcagca ggccgtgtcc gaaacgtcgg ctgtcggtca gttccaaatg tccgccgtat
    33721 tttttggcaa gcgtgtcggc aatcgacagc cccagccccg tcccctgctg ctccgttccc
    33781 aaaatacggt aaaacggatc gaggacgcgg gcgcgttcgg attcgggaat gccgttcccg
    33841 ttatcttcca cccacacggc gagatatttc ccttcgtccg tgaaacccaa atcgatcctg
    33901 ccttcgggcg gcgtgtaacg taccgcgttg tcggcaaagg ttttaatcag cgtatagatt
    33961 tccgtttcgt cggcagacac ttcgacatcg cctccgaccg ccacaccgat gtcctgacgt
    34021 ttttccaaag ccagcggcat cagttcctgc aacacttggt ggaaacggct ttgcagaccg
    34081 aatgtcgttt tcgtcagagg ggtttcgtcc gactgcgaac gcgccagtgc caaaagctgt
    34141 tcgaggaggt gtttgttgcg cctgatgctc tgctgcaaaa cggcaggctg ccgcgctgca
    34201 tcgggtggga gcgacatatt gttgagccgt tccgcctgaa gggaaagggc tgtcatcggc
    34261 gtacgcaatt cgtgtgccgc gtcggcgaca aaccgctgac ggtggcggat gtcttcatcc
    34321 gcacgtttca aaagcaggtt gatggcggtt acgaaacctc tgatttcact gggaatattg
    34381 tccacactca aggcagacag gccattgatt cggcgttgtt cgagactttg cgacaatttg
    34441 cggacggggc gcatggcttt gtgcgtaatc cacacggtca gcaaaatcat cagcggcagt
    34501 gccgccaaca ggggcaacac gctttgccgt gccgcatccg ccgccaaatc ttcacggtat
    34561 tcgttttcct gcataacggc aatccgtccc tgttcggtcg tgcggatata gacgcggtaa
    34621 taatcgtcgt ccccgtccgc ctgaagcgtg tgcagaccgt ccgccagatg cgcaggcagg
    34681 ctgacaacag ggtcttcctg ctgcggcatc tgcacgaaaa tgcgcgtatc gccgtcgccc
    34741 tcgggcaaag tttcgggttt agaatcgggt gcgacgtaca atgccgcctg acggagcagg
    34801 tcgtcctgca acgcttccgt ttcgtggaag gtttcgtagt aggaaaacat acctgcaagc
    34861 attgccagcg gaacaaacat ccaaaccgtc ccgccccggc aaacccaaat ccagcagcat
    34921 caagtcataa ggctgggcag cggcagccgc gcgccgtttt tgacccaatc caccgcatag
    34981 ccgccgtctt tcaaacttgc cgacaccgcc tccgcaatca tcgcatcgtc ttccaccagc
    35041 aaaacacgca tcaactttcc cttcaaaata aaccgtgcct attctaacac cccaaaatta
    35101 gccgcaattt agcggccttt acgcttgccg gtatttttca aaactgcggc acaaaaaacc
    35161 gcgccggcaa ctgccttcag acggcattgg ggcgcgattg caacacacgg gcaggcagag
    35221 cctgcgacag accacaggaa cgattcaggc ttcagacggc ttcgccgttt acggcagagg
    35281 cacgattcct gccgctatcg aactggccaa tatcgccagc gacaaacccc acgcccagaa
    35341 aaacgaataa cggatgtgtt tgcccatcga caatttcgcc aaacccaagc ccatccacaa
    35401 agccggcgaa agcggcgtaa caaaagtgcc gacgatactg ccgatcaaca tcgcataacc
    35461 tgctgcttcg ggcgccacgc ccgcctgcga ggtaatctgc tccacaatcg gaaacagtcc
    35521 gaaataataa gcgtccgtac tcaaaaccaa ctcaaacgga atgcccaaca caccgatggc
    35581 aatatgcaga taaggcagca gcgcgtccgg caggatatgc acaatgtctt tggaaatcgc
    35641 gtccaacatc cccgcaccct tcaaaatccc caaaaacgta cctgccgcca aaataatgga
    35701 cgccatcatc accgcgccgc cggcgtgggc ataaatccgc tccatctgtt cctgcgggct
    35761 gcggtaattc aaaagcaacg ccgccgttgc agccagcata aatacataac ccggtgggaa
    35821 gatgcccgaa aaaagcaggc tcatcgccgc caaaaacagc aggacattcc accaaaacag
    35881 tttcggacgc gccaattttt gttcttcttc cgacaaaggc accggcttta tcaaatccgc
    35941 cacggcgggc aacgcgccca actcccggac aatccgcctt ttttcacgca cacccaaaag
    36001 cagggacagc gcaaggataa acaccacacc gataatttgc accgtcaaca aaggtttata
    36061 caattcgccc acatctgcgc ccaacacgct tgcaacccgc ccggtcggcc cgccccacgg
    36121 cagaaggtta atcaatcccg cactggaagt cagcagcaaa aacagcaggt aaggattcat
    36181 atgcagacgc ttgtaaagcg gcaaaagggc ggggacgacc aataaaaacg tcgtcgcacc
    36241 cgccccgtcc aactgcgcca ccaccgacac caagaccgtc cccacactca ctgccacgat
    36301 attaccccga gtcagcttaa tcaaaccgcc tatcatcgga cggaacagcc ccacatcgtt
    36361 catgattcca aaaaacaaaa tggaaaacat aaacataatc acaatctgca tcaccgattt
    36421 ggtgccgccc gaataaaatt cttttaattg ggatacatca aaccccgcca gcaacgcccc
    36481 aaacagcggc accaagatta atgcgatgat gggcgacact ttttccgtca gcagcaacca
    36541 tacgatgacc ccgataatca gcagtccgat aaacgtcagc atcatttctc ctttatttta
    36601 ttttaaacag aaaaccgacc gtgcaggcaa aaccgcccac agacgcggga taagccctgc
    36661 attctacttt tttattttga aacaagtcaa tcggtcattt cctcccattc acgcctgccg
    36721 ccattcctgc atccgtccgt catttcacgg cggcaaccga tacggaacaa ccggcaaacc
    36781 ggtatcggga cggcgcgggg gcattcatcc cggtgcgccg attcaaacga aaccgcccct
    36841 atcattgcgg agcgcggggc gtgccgtaca cgcgggattt tatagtggat taacaa
//
LOCUS       DAWFJR010000011        36084 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWFJR010000011 DAWFJR010000000
VERSION     DAWFJR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 36084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36084
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.011"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            82..1047
                     /locus_tag="WGR04_000468"
     CDS             82..1047
                     /locus_tag="WGR04_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0794086.1"
                     /translation="MLRLTALTLCTVLALGACSPQNSDSAPQAKEQAVSAAQSESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            1211..2179
                     /locus_tag="WGR04_000469"
     CDS             1211..2179
                     /locus_tag="WGR04_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0794087.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSTE
                     LMLVSRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     EFEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFVGLVVPNIISRLIGDRLRQSLPTVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVMGTALFLWLLLRKPAHAV"
     gene            2169..3143
                     /locus_tag="WGR04_000470"
     CDS             2169..3143
                     /locus_tag="WGR04_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ0794088.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCILFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELVVMMGGSLLLFYTLIKQGGRDLSRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLSIGALILLISAAAVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            3188..3814
                     /locus_tag="WGR04_000471"
     CDS             3188..3814
                     /locus_tag="WGR04_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794089.1"
                     /translation="MTQEHFPSFFNQAPTITVQDALAEFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGAELPQRGDIEAFMQGGRDEGTTGVTASVVQLLTG
                     AAPETGFGGVGPAGRFARRHLLSFGAGEINGTLALRRRDTGKTVAVSLNAALQPFAPK
                     MRDIMPKAVSGSASADELKQFGGLWQERVRAFLIDQADNPEFVTVKEI"
     gene            3895..4653
                     /locus_tag="WGR04_000472"
     CDS             3895..4653
                     /locus_tag="WGR04_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0794090.1"
                     /translation="MITIRNVSHTIGSNPILNDVSLDIPEGGITALIGPNGAGKSTLL
                     SFMARLQPLVHGNISYAGKDIKTTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPSETDKTIVEDALAEFHLQDFADRYLTELSGGQRQRAMIAMVFCQRTDYVLLD
                     EPLNNLDMYHARSLMQILRRLTDEHKRTTVVVLHDINQAAAYADHVVAMKNGQAVLTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
     gene            complement(4712..5050)
                     /locus_tag="WGR04_000473"
     CDS             complement(4712..5050)
                     /locus_tag="WGR04_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ0794091.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            5145..5357
                     /locus_tag="WGR04_000474"
     CDS             5145..5357
                     /locus_tag="WGR04_000474"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794092.1"
                     /translation="MPETVIPAQAGIPISAFQQYLKVSVNPNFHIPNRAETTAFDLKT
                     YGSSCPPLYFLQLSAYSALGKNQKNR"
     gene            5514..9476
                     /gene="purL"
                     /locus_tag="WGR04_000475"
     CDS             5514..9476
                     /gene="purL"
                     /locus_tag="WGR04_000475"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ0794093.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDRQKQQWAALLHDRMTESVLTDIDAAAQLFHHIQSET
                     FSSVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFELREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPIDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHADDIDITEAAYRVLHLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVQDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNDRSALFADVKFDVNEDIAAPFVNSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFADPDTLTLGVCNGCQMVSNLAEIIPGTAGWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNP
                     NGSPQGIAGITNADGRVTIMMPHPERVYRAAQMSWKPEGWTELSGWYRLFAGARKALG
                     "
     gene            9571..10329
                     /gene="gloB"
                     /locus_tag="WGR04_000476"
     CDS             9571..10329
                     /gene="gloB"
                     /locus_tag="WGR04_000476"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ0794094.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLDFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            10636..15129
                     /gene="mspA"
                     /locus_tag="WGR04_000477"
                     /gene_synonym="ausI"
     CDS             10636..15129
                     /gene="mspA"
                     /locus_tag="WGR04_000477"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
                     /protein_id="HEZ0794095.1"
                     /translation="MRFTHTIPFYSVLSTLGLFAVSPAYSSIVRSDVDYQYFRDFAEN
                     KGAFAVDASNISIQDKQGKILGRVLSGIPMPDFRVSNRQTAIATLVHPQYVNSVKHNV
                     GYGSIQFGNDTQNPEEQAYTYRLVSRNPHPDYDYHLPRLNKLVTEISPAALSSVPLLG
                     NGQPKANAYLDTDRFPYFVRLGSGTQQVRKADGTRTRTAPAYQYLTGGTPLKVLGFQN
                     HGLLVGGSLTDQPLNTYAIAGDSGSPLFAFDKHENRWVLAGVLSTYAGFDNFFNKYIV
                     TQPEFIRSTIRQYETRLDVGLTTNELIWRDNGNGNSTLQGLNERITLPIANPSLAPQN
                     DSRHMPSEDAGKTLILSSRFDNKTLMLADNINQGAGALQFDSNFTVVGKNHTWQGAGV
                     IVADGKRVFWQVSNPKGDRLSKLGAGTLIANGQGINQGDISIGEGTVVLAQKAASDGS
                     KQAFNQVGITSGRGTAVLADSQQIKPENLYFGFRGGRLDLNGNNLAFTHIRHADGGAQ
                     IVNHNPDQAATLTLTGNPVLSPEHVEWVQWGNRPQGNAAVYEYINPHRNRRTDYFILK
                     PGGNPREFFPLNMENSTSWQFVGNNRQQAAEQVAQAENARPDLITFGGYLGENAQTGK
                     AAPSYSKTNIEWVNSPNTPHKPSPYTNTPTRTGAGVPTITASKKAATPAPTSHGMPTA
                     PKTGNFSDITGEAAIEKTRHIANAAVYGRPEYRYNGALNLHYRPKRTDSTLLLNGGMN
                     LNGEVLIEGGNMIVSGRPVPHAYDHQAKREPVLENEWTDGSFKAARFTLRNHARLTAG
                     RNTAHLDGDITAYDLSGIDLGFIQGKTPECYRSYHSGSTHCTPNAVLKAENYRALPAT
                     QVRGDITLNDRSELRLGKAHLYGSIRAGKDTAVRMEADSNWTLSQSSHTGALTLDGAQ
                     ITLNPDFANNTHNNRFNTLTVNGTLDGFGTFRFLTGIVRKQNAPPLKLEGDSRGAFQI
                     HVKNTGQEPQTTESLALVSLNPKHSHQARFTLQNGYADLGAYRYILRKNNNGYSLYNP
                     LKEAELQIEATRAEHERNQQAYNQLQATDISRQVQHDSDATRQALQAWQNSQTELARI
                     DSQVQYLSAQLKQTDPLTGILTRAQNLCAAQGYSVDICRQVAKAADTNDLTLFETELD
                     TYIERVEMAESELDKARQGGDAQAVETARHAYLNALNRLSRQIHSLKTGVAGIRMPNL
                     AELISRSANTAVSEQAAYNTGRQQAGRRIDRHLTDPQQQNIWLETGTQQTDYHSGTHR
                     PYQQTTNYAHIGIQTGITDRLSVGTILTDERTNNRFDEGVSARNRSNGAHLFVKGENG
                     ALFAAADLGYSNSRTRFTDYDGAAVRRHAWDAGINTGIKIDTGIKIDTGINLRPYAGI
                     RINRSNGNRYVLDGAEINSPAQIQTTWHAGIRLDKTVELGQAKLTPAFSSDYYHTRQN
                     SGSALSVNDRTLLQQAAHGTLHTLQIDAGYKGWNAKLHAAYGKDSNTARHKQAGIKIG
                     YNW"
     gene            15361..16815
                     /gene="mgtE"
                     /locus_tag="WGR04_000478"
     CDS             15361..16815
                     /gene="mgtE"
                     /locus_tag="WGR04_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ0794096.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNVVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITVDEMVDVIREESEADMLNMAGLQEEEDLFAPVWDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(16901..17809)
                     /gene="hslO"
                     /locus_tag="WGR04_000479"
     CDS             complement(16901..17809)
                     /gene="hslO"
                     /locus_tag="WGR04_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ0794097.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGRGRLKMLVAEAASDRTVRATARWDET
                     AEIADDESLGDLLGEGGVFVLTLQPKDGEPWQGVVPLEGGGIAQMLANYMKRSEQIDT
                     HIVLSASDEAAGGLLVQRLPEAVLDEEAWEHVSTLARTLTADELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            18054..18809
                     /locus_tag="WGR04_000480"
     CDS             18054..18809
                     /locus_tag="WGR04_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0794098.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            18828..19046
                     /locus_tag="WGR04_000481"
     CDS             18828..19046
                     /locus_tag="WGR04_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794099.1"
                     /translation="MSMPEIPKWYDDDGQIVSCTEKIKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            19191..19535
                     /locus_tag="WGR04_000482"
     CDS             19191..19535
                     /locus_tag="WGR04_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ0794100.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            19535..20227
                     /locus_tag="WGR04_000483"
     CDS             19535..20227
                     /locus_tag="WGR04_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ0794101.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            20294..21514
                     /gene="argJ"
                     /locus_tag="WGR04_000484"
     CDS             20294..21514
                     /gene="argJ"
                     /locus_tag="WGR04_000484"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ0794102.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVVD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQSAATVYTCD
                     LSHGYVSINADYRS"
     gene            21574..22983
                     /locus_tag="WGR04_000485"
     CDS             21574..22983
                     /locus_tag="WGR04_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ0794103.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGVSIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            23173..24564
                     /gene="hrpA"
                     /locus_tag="WGR04_000486"
     CDS             23173..24564
                     /gene="hrpA"
                     /locus_tag="WGR04_000486"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ0794104.1"
                     /translation="MFPDFSQTLSKDRHFLRSAFKNPNKYGGLSKVEEKYQKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKAIAENQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSQHFNGAPVLEVSGRTYPVEILYRPLIGKDEDDAEIELTDAIVDAADELARYGEGDI
                     LVFLPGEREIRKAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEENFNSRPEFTDPEIVRSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            24665..25792
                     /locus_tag="WGR04_000487"
     CDS             24665..25792
                     /locus_tag="WGR04_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794105.1"
                     /translation="MKHLLIDFENVQPQNLDKLPTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            25970..27547
                     /gene="lpt3"
                     /locus_tag="WGR04_000488"
     CDS             25970..27547
                     /gene="lpt3"
                     /locus_tag="WGR04_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HEZ0794106.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FVIAALYLFARYKATRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEITEVGG
                     AGASMLDKLWLPALWGMLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQSAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTQLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCSEGSVTGNLITGD
                     AGSLNIHDGKAEYVYPQ"
     gene            27613..30888
                     /locus_tag="WGR04_000489"
     CDS             27613..30888
                     /locus_tag="WGR04_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEZ0794107.1"
                     /translation="MQNMKNQIRGSGMDARSNPANVSDGLQNSSGHIGTNTRYRLTKL
                     GEQMARLPIDPKIARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHER
                     FTDKQSDFLAYLNIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQT
                     AIEMGLTTKEAAFRRLSEIKQLTSSENQGDQDLSAKRKQKQLDKKQHRAQIRAAKEAG
                     YEQIHRALLTGLIANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVET
                     TKLYARDVAVIQPEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRP
                     VPYGKVAPEEAREIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDD
                     EALFAFYHERLPDFYTADAVSDGLRPANPQQTAPSYAREERREGKTVAAQTNFSATAA
                     NPLPQEREQSASASTFSDDLRPANLQQTAPSPVGEGWGEGKTVATQTNFSATSTNPLP
                     QEREQSASASTFSDDLRPANLQQPSPSPVGEGWGEGKTVATQTNFSATTANPLPNPLP
                     QEGEQSAAASAVSNDPQPQKQPAPQKDRLKPLPLADIRTFQAWLKTAERENPRLLFLS
                     RDDLMQHAAAHITEEQFPKFWQTADGKFELSYRFEPHHPLDGVTLTLPLTVLNRLHAP
                     SLEWLVPGMLREKIQLLIKALPKQIRRICVPVPDFITQFLSQNPDRNAPILPQLAQAI
                     AKTAGDIRILEQINQDEWAAFRLPEHCYFNLRIIDDGGQELAGGRKLHELQQQLGQAA
                     AVTFRDNTQEFERDNVTTWDIGTLPESIKFARGKQQLTGYLGLQKEKDGRIALRLCDT
                     TEAAEQAHRQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVC
                     DRAFIGEDELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNGKLGKHPL
                     THLLRLRLQTLLAAGFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQE
                     LEQMWQEKNDGLVKQGLPVSDDLTAFKWMIEELRVSLFAQELKTPYPVSVKRLLKEWE
                     DLN"
     gene            complement(30911..31333)
                     /locus_tag="WGR04_000490"
     CDS             complement(30911..31333)
                     /locus_tag="WGR04_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEZ0794108.1"
                     /translation="MTDIFTTSKRSFVMSKIHSKETKPEVLVRKFLFFQGFRYRKNDK
                     RYVGKPDIVLSKYKTVVFIHGCFWYGHSCNKGHIPKSNTDFWLEKITKNCERDIKNET
                     ELEKIGFKVIVVWECELKNKAICRERLNRLVREIKDAV"
     gene            complement(31337..32983)
                     /locus_tag="WGR04_000491"
     CDS             complement(31337..32983)
                     /locus_tag="WGR04_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HEZ0794109.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFGLGNKIEISTIQGNERVTFTLDYAEIRRSKGIYQPEFRKESVESNIESGTTIT
                     LTELTKKQGYPLDNYVEHLSRLFDFPAQDFKIKVSLNGSEPKIIDGNLKYDLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHFYSYLTGWLNVDFIDNDDEDLIATDRTSLDWKHSKTSKLRVFLGEIVRAI
                     GQDWRKRRKKEKDDEVKGESGVDIEDWKNKLPEKEREPVEVILNRLEDSELTNKEQAE
                     VISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFEDKVQKQTG
                     LHSIDGRELIEQAFGSKNSILLLTNNKTKAEQNLEDGLEQLACGTWTGFRNPVQHELR
                     ANLSPSIFNDKDALDLISLVSYLLRKVEQTKKRSKVVSSK"
     gene            complement(32997..34052)
                     /locus_tag="WGR04_000492"
     CDS             complement(32997..34052)
                     /locus_tag="WGR04_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0794110.1"
                     /translation="MKNSKLKAVDFFCGGGGMSYGMQSAGIQVLAGIDYEPSCKETYE
                     ANINAKFIQADVFELQPETLEKELGLKKNDDDLILIGCSPCQYWSVIQTDKRKSEKSK
                     SLLLEFQRFVEYFNPGYVVVENVPGILSRMKESRLDNFIKLLEEKGFTVHFGIHNTAD
                     YGIPQSRKRFTLIANRITKEKLEPVKYSGKRLTVRDVLGMENGFPKIIAGHQDETDFM
                     HSCAGLSDINLKRLALIPKNGGNRLAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPA
                     PTITTKFFSISNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVP
                     PMYAEKIGRAIVDNIEC"
     gene            34342..34494
                     /locus_tag="WGR04_000493"
     CDS             34342..34494
                     /locus_tag="WGR04_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794111.1"
                     /translation="MSGIYLPRLFPPHIAERGLLYFQQGKVLDVRKTSAGHYRAEVCG
                     SENYWV"
     gene            34864..35019
                     /locus_tag="WGR04_000494"
     CDS             34864..35019
                     /locus_tag="WGR04_000494"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ0794112.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(35109..35558)
                     /locus_tag="WGR04_000495"
     CDS             complement(35109..35558)
                     /locus_tag="WGR04_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0794113.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINTVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
     gene            complement(35646..36083)
                     /locus_tag="WGR04_000496"
     CDS             complement(35646..36083)
                     /locus_tag="WGR04_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEZ0794114.1"
                     /translation="MAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARCGLLFATLKG
                     KTMKKMFVLFCMLFSCAFSLAAVNINAASLQELEALPGIGPAKAKAIAEYRAQNGAFK
                     SVDDLTKVKGIGPAVLAKLKDQASVGAPAPKGPAKPVLPADKK"
BASE COUNT         8989 a        10726 c         8498 g         7871 t
ORIGIN      
        1 ttatcattta tcctttctag agccgttccg gtttgtccga ccggcggctt tgccccaata
       61 tccccatttt ggagacaccc tatgttgcgt ttgaccgcat tgaccctctg caccgtcctc
      121 gccttgggcg cgtgttcgcc gcaaaattcc gactctgccc cacaagccaa agaacaggcg
      181 gtttccgccg cacaatccga aagtgcgtcc gttaccgtca aaaccgcgcg cggcgatgtt
      241 caaataccgc aaaaccccga acgtatcgcc gtttacgatt tgggtatgct cgacaccttg
      301 agcaaactgg gcgtgaaaac cggtttgtcc gtcgataaaa accgcctgcc gtatttagag
      361 gaatatttca aaacgacaaa acccgccggc actttgttcg agccggatta cgaaacgctc
      421 aatgcctaca agccgcagct catcatcatc ggcagccgcg ccgccaaggc gtttgacaaa
      481 ttgaacgaaa tcgcgccgac catcgaaatg accgccgata ccgccaatct caaagaaagt
      541 gccaaagagc gtatcgacgc gctggcacaa atcttcggca aacaggcgga agccgacaag
      601 ctgaaggcgg aaatcgacgc gtcttttgaa gccgcgaaaa ctgccgcgca aggcaaaggc
      661 aaaggtttgg tgattttggt caacggcggc aagatgtccg ccttcggccc gtcttcacga
      721 ctgggcggct ggctgcacaa agacatcggc gttccagccg ttgacgaagc cattaaagaa
      781 ggcagccacg gtcagcctat cagctttgaa tacttgaaag agaaaaatcc cgactggctg
      841 ttcgtccttg accgcagcgc ggccatcggc gaagagggtc aggcggcgaa agacgtgttg
      901 aataatccgc tggttgccga aacaaccgcc tggaaaaaag gacaagtcgt ttacctcgtt
      961 cccgaaactt atctggcagc cggcggcgcg caagagctgc tgaacgcgag caaacaggtt
     1021 gcggatgctt ttaacgccgc gaaataatga aacggcggca ttcgatgccg tctgaaacac
     1081 ggatgcaaac cgcctcctgt gtttcagacg gcattgcccg atacggaggc ttcaaacaag
     1141 gctttccgct ccgacggttc ggactgcctt gtttgaatct tctactcctt aacgcttttc
     1201 ccttctgttt atgactgcca aacctttttc cctcaacctg actaacctcc tgctgctggc
     1261 ggtgttgttt gccgtcagcc tgtcggtcgg cgttgccgat ttccgctggt cggatgtgtt
     1321 ttcgctgtcg gacagcaccg aactgatgct ggtcagccgc ctgccgcgca cgtttgcgat
     1381 tgtgctgacg ggcgcgtcga tggcggtggc ggggatgatt atgcagattt tgatgcgcaa
     1441 ccgttttgtc gagccttcta tggcgggcgc gggtcagagt gcggctttgg gtttgcttct
     1501 gatgtccctg ctgctgcctg ccgcaccgct gccggtcaaa atgtcggttg ccgccgttgc
     1561 cgcgctaatc gggatgctgg tgtttatgct gcttatccgc cgtctgccgc cgacggcgca
     1621 actgatggtg ccgctggtcg ggattatttt tggcggcgtg gttgaggcag tggcgacgtt
     1681 tatcgcgtat gagtttgaga tgctgcaaat gttgggcgtg tggcagcagg gcgatttttc
     1741 gagcgtgctg ctggggcggt acgagctgct ttggattacg ggcggtttgg cggtgtttgc
     1801 ctatctgatt gccgaccggc tgacgatttt ggggctgggc gaaacggtca gcgtgaattt
     1861 gggactaaac cggacggcgg tattgtggtc ggggctgatt attgtggctt tgattacgtc
     1921 gctggttatc gttacggtcg gcaatattcc gtttgtcggg ctggtcgtgc cgaacatcat
     1981 cagccgactg ataggcgaca ggctgcgcca aagcctgcct acggtggctt tgttgggcgc
     2041 gtctttggtt ttattgtgcg acattatcgg acgcgtgatt gtgtttccgt ttgaaattcc
     2101 ggtctctacg gtttttggtg tgatggggac ggctttgttt ttgtggcttt tattaaggaa
     2161 acctgctcat gccgtctgaa aaaaatatcg gttttatggc aggaagcagc cgtccgttgt
     2221 gggtcgcctt tgcgctgttg ctggtttcct gcatcctgtt tatgacgctc aacgtcaaag
     2281 gcgattggga ttttgtcttg cacctgcgcc tgaccaaact tgccgcgctg ctgatggtcg
     2341 cctatgcggt cggcgtgtcc acgcaactct tccaaacgct gaccaacaat ccgattctga
     2401 ccccttcgat tttgggtttc gattcgctat atgtgttttt gcagaccttg ctggtgttta
     2461 cgttcggcgg cgtgggctat gcttccctgc cgttgacggg caaattcggc tttgaactgg
     2521 ttgttatgat gggcggttcg ctgctgttgt tctacacgct tatcaaacag ggcggacgag
     2581 atttgtcgcg catgatttta atcggcgtga ttttcgggat tttgttccgc agcctgtcgt
     2641 cgctgctttc gcgcatgatc gatcccgaag aatttacggc ggcgcaggcg aatatgtttg
     2701 ccggattcaa taccgtccac agcgaacttt tgagcatagg cgcgctgatt ctgctcatca
     2761 gcgcggcggc ggtttggcgc gaacgctacc gcttggacgt acaccttttg gggcgcgacc
     2821 aagccgtcaa tttgggcatc agctacacgc gcaacacctt atggatactg ctttggattg
     2881 ccgcgctggt ggcgacggcg accgccgtgg tcggcccggt aagctttttc gggcttctcg
     2941 ccgcctcgct tgccaaccac ttttccccgt ccgtgcgcca ttccgtccgc ctgccgatga
     3001 cggtttgtgt cggcggtatc ctcttggtcg gcggacaaac cgtattcgaa cacttcttgg
     3061 gcatgaaggc ggtattaagc gtggtggtcg aatttgcggg cggactcgtt ttcctctatc
     3121 tcgttttaaa acacaaaaaa tgacggatgc cgtctgaacg gcagcccgcc ccgaaaggac
     3181 aaaccatatg acgcaagaac atttcccatc attctttaac caagccccaa ccattaccgt
     3241 ccaagacgca ttggccgaat tcctcggcgc ggccgaaaac ggcatcctca cttaccgcta
     3301 cgccgatgcc gtgcgcctgt gcggacattc ctgcccgacc gtcgcgggcg cgtacctgat
     3361 ggttatcaaa ggtctgaaag ccctttacgg cgcagagctg ccgcaacgcg gcgacatcga
     3421 agcctttatg cagggcgggc gtgacgaagg cacgaccggc gttaccgctt ccgtcgtcca
     3481 actccttacc ggcgcagccc cggaaaccgg ctttggcggc gtaggcccgg ccggacgctt
     3541 cgcccgccgc cacctcttgt cctttggtgc aggcgaaatc aacggcacac tcgcgctccg
     3601 ccgccgcgat accggcaaaa ccgtcgccgt cagcctcaac gctgcgctcc aaccctttgc
     3661 gccgaaaatg cgcgacatca tgcccaaagc cgtcagcggc agcgcaagtg ctgacgaact
     3721 caaacaattc ggcgggcttt ggcaagaacg cgtccgcgcc ttcctgattg accaagccga
     3781 caatcccgaa tttgtcaccg tcaaagaaat ctaaaacgtc ataatcaata agtttcagac
     3841 ggccctaaaa ctcaaggccg tctgaaaccc caatttccat tttcggaccc gcctatgatt
     3901 accatccgca acgtcagcca caccatcggc agcaacccca tcctcaacga cgtcagcctc
     3961 gacattcccg aaggcggcat taccgcgcta atcggcccga acggcgcagg caaatccacc
     4021 ctcctctcct ttatggcgcg gcttcagcct ctggtacacg gcaacatcag ctacgcaggc
     4081 aaagacatca aaaccacccc caccgccgaa cttgcccgca cgctctccat cctcacccaa
     4141 gaaaacagca tcatgagccg cattaccgtg cgcgacctgc tgatgttcgg ccgctatccc
     4201 taccaccaag gcaggccgtc tgaaacggat aaaaccattg tcgaagacgc gcttgccgag
     4261 ttccacctgc aagacttcgc cgaccgctac ctgaccgagc tttccggcgg ccaacgccaa
     4321 cgcgccatga ttgccatggt gttctgccag cgcaccgact acgttttact cgacgaaccg
     4381 ctgaacaacc tcgatatgta tcacgcccgc tccctcatgc aaatcctgcg tcggctgacc
     4441 gacgaacaca agcgcaccac cgtcgtcgta ttgcacgaca tcaaccaggc agcagcctac
     4501 gccgaccacg tcgtcgccat gaaaaacggc caagccgtcc tgaccggcac gcccgaagaa
     4561 gtgttcaccg tacacaacat caaagaactg ttcgacatgg acgtggacgt actcgactac
     4621 gaaggcaaaa aactcatcgt ccaccatatc tgaagctcaa aaagaaaatg ccgtctgaaa
     4681 ggctttcaga cggcatttcc attccaaacg ctcaaaccgc cgcgtccgca cgttcgcccg
     4741 tgcggatacg gattgcctcc tcgaccggca gcacaaaaat cttgccgtcg ccgattttgc
     4801 ccgaacgcgc cacctcgaca atcacgtcaa tcgcgcgttc cacagcgtca tccgccaaca
     4861 ccagctcgat tttgaccttg ggcaggaaat cgacggcgta ttccgcaccg cgatagattt
     4921 ccgtatgccc cttctgcctg ccgaaccctt tgacctcgct gacggtcatc cccgtaatgc
     4981 cgatttccgt caacgcctcg cgcacgtcgt cgagtttgaa cggtttgaca atcgcctcga
     5041 tttttttcat cacattctcc attaattaac gtaaacaaac gacaaatata gcagaaaccg
     5101 ccggcaaacg ataaacggta ctgttcgccc ggcgatgcgg cgatttgccg gaaaccgtca
     5161 ttcccgcgca ggcgggaatc ccgatctctg catttcagca atatttaaag gtttctgtaa
     5221 atccaaactt ccatattcct aaccgtgcgg aaacgacggc atttgatcta aaaacatacg
     5281 gaagcagttg cccgcccctc tacttcctgc aattatccgc atactcggct ttgggtaaaa
     5341 atcagaaaaa cagataaaac ccttattcct taatatcttc agacggcacc tgtattttcc
     5401 ccaactgtat gccgtctgaa gattgtcaac attttaagcc aaaatatcca agcaatacaa
     5461 acctgtccgg cgtataatcc ctctttttcc caaccgcatt tgtgagccga attatgtctg
     5521 tcgttttgcc cttgcgcggc gttaccgccc tttccgattt ccgtgtcgaa aaactcttgc
     5581 aaaaagccgc cgcactcgga ctgcccgaag tcaaattaag cagcgaattt tggtatttcg
     5641 ccggcagcga gaaagcattg gatgccgcga ctgtcgaaaa actgcaagcc ttgttggcgg
     5701 cgcaaagcgt tgaacaaacg ccaaaagcgc gcgagggctt gcatttgttt ttggtcacgc
     5761 cccgtttggg tacgatttcg ccgtgggctt ccaaggcgac caatatcgcg gaaaactgcg
     5821 gtttggcagg catcgagcgc atcgaacgcg gcatggcggt gtggctggaa ggtgcgctta
     5881 ccgacaggca aaaacaacaa tgggcagccc tgttgcacga ccgcatgacc gaaagcgtgt
     5941 tgaccgacat cgatgcggcg gcgcaactgt tccaccacat ccaatccgaa actttctcca
     6001 gcgtcgatgt tttgggcggc ggtaaagagg ctttggtcaa agccaatacc gaaatgggct
     6061 tggcactctc cgccgacgaa atcgattatc tggttgaaaa ctatcaggct ttgcagcgca
     6121 atccgtccga tgtggaactg atgatgttcg cgcaggcaaa cagcgaacac tgccgccaca
     6181 aaatcttcaa cgccgatttc atcctcaacg gcgaaaaaca accgaaatcc ctgttcggca
     6241 tgatacgcga cacgcacaac gcgcatcccg aaggtacggt cgtcgcctat aaagacaatt
     6301 cgtccgtgat cgaaggcgcg aaaatcgagc gtttctatcc gaatgcggcg gaaaaccaag
     6361 gctaccgttt ccacgaggaa gacacgcata tcatcatgaa agtggaaacg cacaaccacc
     6421 cgaccgccat cgcgccgttt gcgggcgcgg caacaggcgc gggcggcgaa atccgcgacg
     6481 aaggtgcgac gggcaaaggt tcgcgtccga aagcaggctt gaccggcttt accgtgtcca
     6541 acctcaacat ccccgacctc aaacagccgt gggaacaaga ctacggcaag ccggaacata
     6601 tttcctcgcc gttggacatc atgattgaag gcccgatcgg cggcgcggcg ttcaacaacg
     6661 aattcggccg ccccaacctc ttgggctact tccgcacttt tgaagaaaaa tttgacggtc
     6721 aggttcgcgg ctaccacaaa ccgattatga ttgccggcgg cttgggcagc attcaggcgc
     6781 agcagacgca taaagacgaa atccccgaag gcgcgttgct gatccaactg ggcggcccgg
     6841 gcatgcttat cggcttgggc ggcggcgcgg cttcttcgat ggataccggc acgaacgacg
     6901 cttctttaga cttcaactcc gtacaacgcg gcaaccccga aatcgaacgc cgcgcgcagg
     6961 aagtcatcga ccgctgctgg cagctcggcg acaaaaaccc gattatctcc atccacgacg
     7021 tgggcgcagg aggtttgtcc aacgccttcc ccgaactggt caacgatgcc ggacgcggcg
     7081 cggtattcga gctgcgcgaa gtgccgttgg aagaacacgg tttaacgcct ttgcaaatct
     7141 ggtgcaacga atcgcaagag cgttatgtgt tgtcgatttt ggaaaaagat ttggatacct
     7201 tccgcgccat ctgcgaacgc gaacgctgcc cgtttgccgt ggtcggtaca gcgaccgacg
     7261 acggtcattt gaaagtgcgc gacgatttgt tcgccaacaa ccccatcgat ttgccgttga
     7321 acgtcttgct cggcaaaccg cccaaaacca cgcgtaccga caaaacagtt acgccatcta
     7381 aaaaaccgtt tcacgccgac gatatcgaca tcaccgaagc cgcctaccgc gttttgcacc
     7441 tgcctgccgt agccgccaaa aacttcctga ttaccatcgg cgaccgcagc gtcggcggca
     7501 tgacccaccg cgaccaaatg gtcggcaaat accaaacccc cgtagccgac tgcgccgtta
     7561 ccatgatggg cttcaatacc tatcgcggcg aagcaatgtc tatgggcgaa aaaccgaccg
     7621 tcgccctgtt tgacgcgcct gcttcgggca gaatgtgcgt cggcgaagcc atcaccaaca
     7681 tcgcggcggt caacatcggc gacatcggca acatcaagct ctccgccaac tggatggcgg
     7741 cgtgcggcaa cgaaggcgaa gacgaaaaac tctaccgcac cgtcgaagcg gtttccaaag
     7801 cctgtcaggc attggatttg agcattcccg tgggcaaaga cagcctgtcg atgaaaaccg
     7861 tatggcagga tggcgaagag aaaaaatccg tcgtttcgcc gttgagcctg attatctccg
     7921 cgttcgcgcc ggttcaagac gtacgcaaaa ccgttacgcc cgaattgaaa aacgtcgagg
     7981 acagcgtatt gctgtttgtc gatttgggct tcggcaaagc gcgtatgggc ggctctgcat
     8041 tcggtcaggt atacaacaac atgagcggcg acgcgcccga tttggacgac acaggccgtc
     8101 tgaaagcgtt ttacaacgtg attcaacagc ttgtcgccga agacaaactc ttggcatacc
     8161 acgaccgcag cgacggcggc ttgtttgccg ttttggtaga aatggcgttt gcggggcggt
     8221 gcggcttgga tatagattta aatttattgc ttgcacaaac atttattacc aaccataccg
     8281 ctctgtctca atcattgcgg actgaagagg taaaagcgtt ggctgaatgg caagaaacca
     8341 ttgcccgcac attatttaat gaagagttgg gtgctgttat ccaagttaga aaacaagatg
     8401 ttgccgatat tatcaattta ttctatcaac aacagctgca tcataatgtc tttgaaatcg
     8461 gtacgttaac tgatgagaac acgttaatca tccgcgacgg gcaaacgcac cttatttctg
     8521 acaacctaat caaactgcaa caaacctggc aagaaaccag ccatcaaatc caacgcctgc
     8581 gcgacaaccc cgcctgcgcc gacagcgagt tcgccctgat tggcgacaac gaccgcagcg
     8641 cattgtttgc cgacgtgaag tttgacgtga acgaagacat cgccgcacct ttcgtcaaca
     8701 gcggcgcgaa acccaaaatc gccatcctgc gcgaacaggg cgtaaacggg caaatcgaaa
     8761 tggccgccgc gttcactcgc gccggtttcg atgcctacga cgtgcatatg tccgacctga
     8821 tggcaggccg cttccgcctt gccgacttca aaatgctggc ggcgtgcggc ggtttcagct
     8881 acggcgacgt actcggcgcg ggcgaaggct gggcgaaatc gattctgttc caccctgctc
     8941 tgcgcgacca gtttgccgcc ttcttcgccg acccggacac gctgacattg ggcgtgtgca
     9001 atggctgcca aatggtcagc aaccttgccg aaatcatccc cggcacggca ggctggccga
     9061 agttcaaacg caacctgagc gaacagtttg aagcgcgcct aagcatggtt cacgtcccca
     9121 aatccgcgtc gctgattctg aacgaaatgc aaggctccag cctgcccgtc gtcgtcagcc
     9181 acggcgaagg ccgcgccgac ttcgcacttc acggcggcaa catttctgcc gatttgggca
     9241 ttgcgctgca atatgtggac ggtcaaaacc aagttaccca aacctatccg ctcaacccca
     9301 acggctcgcc gcaaggcatc gccggcatta ccaacgccga cggccgcgtg accatcatga
     9361 tgccccaccc cgaacgcgtg taccgcgccg cacaaatgag ctggaaaccg gaaggctgga
     9421 cggaactgtc cggctggtac cgcctctttg ccggcgcacg taaagccttg ggctaaccgc
     9481 cctactcaaa ccaatgccgt ctgaagaata tttcagacgg cgttccggca taccatcctt
     9541 taaacggtat ccgtccaccg aggaacactc atgaaaatca cccccgtcaa agccctaacc
     9601 gacaactaca tttggatgat acagcacggc aaccatgccg tctgcgtcga cccttccgaa
     9661 ccctcgcccg tcttggaatt cctcgtccgc aaccgcctca tgcttgccca aacatgggta
     9721 actcaccccc atcccgacca cgagggcggt gcggcggcac tctggcgcgg ctacatggaa
     9781 tcgcccgttt acggcgaatc cgacatcgaa gcagcaaccc acaccgtaac cgccggcacc
     9841 caattcacct tcggcgacgg acaggttacc gtttgggcaa cacccggcca cacagaccgc
     9901 cacaccagct accttctcga aacttcagac ggcatacacg ttttctgcgg cgacaccctg
     9961 ttttccgccg gctgcggacg cgtgtttacc ggcacaatcg aacagcttta cgacagcttc
    10021 caacgcttca accgcctgcc tgaaaacacc ctgttctacc cggcacacga atacaccgcc
    10081 gccaacctgg attttgccgc ccatatcgag ccggacaacg ccgacattca gacggcactg
    10141 aaagcggcgg cgcatacgcc taccctgccc gttaccctcg cgcacgaacg ccgcgtcaat
    10201 ccgtttttgc gcgtcgacct gccgcacgtc agagaccgcg ccgaggcatt gagcgggaaa
    10261 acgttaaaca gcagcctcga tacctttgtc gcgctgcgtg aacttaaaaa ccaataccgg
    10321 acgaaataaa acaacgggaa aacgcagcca ttcctaggat ttttattaaa atcttaaata
    10381 aaatcataca atcatcgcca atagacgaaa ggacaccgtt gccttataat caaacaaaaa
    10441 caaaatatat aatatagtgg attgaattta aatcaggaca aggcgacgaa gccgcagaca
    10501 gtacagatag tacggcaagg cgaggcaacg ccgtactggt ttttgttaat ccactatata
    10561 aatccagcac aaaacgggat cggtgattct tgtccgcaag aatcgttgat tttctctatt
    10621 acacggataa tcatcatgcg cttcacacat accatcccat tttattccgt attgtccacc
    10681 ctcggtcttt ttgccgtttc ccctgcttac tcatccattg tccgcagcga tgtcgattac
    10741 caatattttc gcgactttgc cgaaaataaa ggcgcgttcg ccgtagatgc aagcaatatt
    10801 tccatccaag acaagcaagg caaaatatta ggcagggttc tcagcggcat ccccatgccc
    10861 gacttccgcg tcagcaaccg gcaaaccgct atcgccaccc tggttcaccc ccaatacgtc
    10921 aacagtgtca aacacaacgt cggctacggt tccatacaat tcggcaacga cacccaaaac
    10981 cctgaagaac aagcctatac ctaccgcctc gtatcacgca acccgcaccc ggactacgac
    11041 taccaccttc cccgcctcaa caaactggtt accgaaatct cacctgccgc actcagcagc
    11101 gtacccttgc ttggaaacgg ccagccaaag gccaatgcct acctcgatac cgaccgcttc
    11161 ccatactttg tacgactcgg ctcaggcacg caacaagtcc gcaaagcaga cggcacgcgt
    11221 acacgaaccg ccccggcata ccaatacctg accggcggca cgccgctgaa agtattgggg
    11281 ttccaaaacc acggcttact cgtcggcggc agcctgaccg accaacccct taacacctac
    11341 gcaatcgccg gagacagcgg ttcccccctg tttgccttcg acaagcatga aaaccgctgg
    11401 gtgcttgcgg gcgtactcag cacctacgcc ggcttcgaca acttcttcaa caaatacatc
    11461 gtcacgcaac ccgaattcat ccgttccacc atccgccaat acgaaacccg gctggatgtc
    11521 gggctgacca ccaacgaact catatggcgc gacaacggta atggcaacag caccctgcaa
    11581 gggctcaacg aacgcatcac cctgcccatt gcaaaccctt cgcttgcccc acaaaacgac
    11641 agcaggcaca tgccgtctga agatgccggc aaaacgctca tcctatccag caggttcgac
    11701 aacaaaacac tgatgctggc agacaatatc aaccaaggcg caggcgcatt gcagttcgac
    11761 agcaacttca ccgtcgtcgg taaaaaccac acatggcaag gtgcaggcgt tatcgtagcc
    11821 gacggcaaac gcgtcttctg gcaagtcagc aaccccaaag gcgaccggct ctccaaactg
    11881 ggcgcaggca cgcttatcgc caacggacaa ggcatcaacc agggcgacat cagcatcggg
    11941 gaaggcactg tcgtactcgc ccaaaaagct gcttcagacg gcagcaaaca agcattcaac
    12001 caagtcggca tcaccagcgg caggggcacg gccgtcctcg ccgacagcca gcaaatcaaa
    12061 cccgaaaacc tctatttcgg cttcaggggc ggacggctcg acctcaacgg caacaacctt
    12121 gcctttaccc atatccgcca tgcggacggc ggcgcgcaaa tcgtcaatca caaccctgac
    12181 caagccgcga cactgacgct gaccggcaac cccgtcctca gtcccgagca tgtcgagtgg
    12241 gtgcaatggg gcaaccgtcc gcaaggcaac gcggcggttt acgaatacat caacccgcac
    12301 cgcaaccgtc ggaccgacta cttcatactc aaacccggcg gcaacccgcg cgaatttttc
    12361 ccgttaaata tggaaaactc aacaagctgg caatttgtcg gcaacaacag gcaacaggcc
    12421 gccgaacaag tcgcccaagc cgaaaatgcc cgccccgacc tgattacctt cggcggatac
    12481 ttgggtgaga acgcgcaaac gggtaaagcc gcaccgagtt acagcaaaac caatatcgaa
    12541 tgggtgaata gtccaaacac cccacacaag ccctcgccct atacgaatac gccaacccgc
    12601 acaggggcag gcgtaccgac gattaccgcc tcaaagaagg cggcaacccc ggcgcctact
    12661 tcccatggga tgcctacagc acccaagact gggaatttct cggacataac cggcgaagca
    12721 gccatagaaa aaacccgcca tatcgcaaat gccgccgtat acggccggcc cgaataccgt
    12781 tacaacggcg cactcaacct gcactatcgt cccaaacgca ccgacagcac gctgttgctc
    12841 aacggcggca tgaaccttaa cggggaagtc ttgattgagg gcggcaatat gattgtgtca
    12901 ggcaggcccg taccccatgc ctacgaccac caggccaaac gcgaacccgt tcttgaaaac
    12961 gaatggaccg acggcagctt caaggctgca cggttcaccc tgcgaaacca tgcccgactg
    13021 acggcagggc gcaataccgc gcatctggac ggcgacataa ccgcatacga tctgtccggc
    13081 atcgacctcg gctttatcca aggcaaaaca ccggaatgct accgctccta ccatagcggc
    13141 agcacccact gcacacccaa cgccgtttta aaagccgaaa actatcgtgc actacctgca
    13201 acgcaagtac gcggcgacat tacccttaac gaccgttcag agctccgcct gggcaaagca
    13261 cacctgtacg gcagcatccg tgccggcaaa gacaccgcag tccgcatgga agcagacagc
    13321 aactggacac tttcccagtc cagccacacc ggcgcactga cgcttgacgg cgcacaaatt
    13381 accctgaacc ccgatttcgc caataataca cacaacaacc gcttcaacac actgaccgtc
    13441 aacggcacac ttgacgggtt cggcacattc cgattcctga ccggcatcgt ccgaaaacaa
    13501 aatgcccccc ccctcaaact ggaaggggac agccgcggcg cattccaaat ccacgtcaaa
    13561 aacaccggac aagaacctca aacaaccgaa tcgcttgcac ttgtgagcct caatccgaaa
    13621 cacagccacc aagcccgatt caccctccaa aacggctatg ccgatttggg tgcctaccgc
    13681 tacatcctcc gcaaaaacaa caacggatac agcctgtaca acccgctcaa agaggccgaa
    13741 cttcaaattg aagccacgcg tgcggaacat gagcgcaacc aacaggcata caaccaatta
    13801 caggcaaccg acatcagcag acaggttcaa catgactctg acgcgaccag gcaggcacta
    13861 caggcctggc agaacagtca aaccgaactt gcccgcatcg acagccaagt ccaatatctg
    13921 tccgcccaat tgaaacagac agacccgcta accggcattc tgacgcgtgc ccaaaacctg
    13981 tgtgccgcac aaggatacag tgtcgatatc tgccgtcagg ttgccaaagc cgccgacacg
    14041 aacgacctga cactcttcga aaccgaactg gatacgtata tagaacgtgt agaaatggcc
    14101 gaatccgaac ttgacaaagc acggcaaggc ggcgatgcgc aagccgtcga aacagcccgg
    14161 cacgcctacc tgaacgcact caaccgtctg tcccgacaaa tccacagttt gaaaaccggc
    14221 gttgccggca tccgtatgcc gaacctggcc gaactgatca gccggtcggc caacaccgcc
    14281 gtttccgaac aggccgccta caataccggc cggcaacagg cgggacgccg catcgaccgc
    14341 caccttaccg atccgcagca gcaaaacatc tggctggaaa ccggtacgca acaaaccgac
    14401 taccatagcg gcacacaccg tccctaccaa caaactacca actatgcaca tatcggcatc
    14461 caaaccggca tcaccgaccg tctcagtgtc ggtacgattt taaccgatga gcgcacaaac
    14521 aaccgttttg atgaaggcgt atccgcccga aaccgcagca acggcgcaca tctgttcgtc
    14581 aaaggggaaa acggcgcact ctttgccgcg gcagatttag gctacagcaa cagccgtacc
    14641 cgatttaccg attatgacgg ggctgccgtc cgccgccacg catgggatgc aggcatcaac
    14701 accggcatca aaatcgatac cggcatcaaa atcgataccg gcatcaacct cagaccctat
    14761 gccggcatcc gtataaaccg cagcaacggc aaccggtacg tactcgacgg cgcagagata
    14821 aacagcccgg cgcaaatcca aaccacatgg catgccggca tccgtctcga taaaaccgtc
    14881 gaactgggtc aagccaagct gacccccgct ttcagcagcg attactacca tacccgccaa
    14941 aacagcggtt ccgccctcag cgtcaacgac cgtaccttac tgcagcaagc cgcccacggc
    15001 acactgcata ccctgcaaat cgacgccgga tacaaaggct ggaacgccaa acttcatgcc
    15061 gcttacggca aagacagcaa caccgcccgc cacaaacagg caggaatcaa aataggctac
    15121 aactggtaac aagccgataa aaatgccgtc tgaaacccgc gtttcagacg gcatttgcgt
    15181 taaaaatagt aaaccgttcc aaaagggagt agaatagtgc cgtttccaac cctgcgcctg
    15241 taccgtcagg cttttattat ggaccttccc agttcgcttt tactgaacgc cccttccgat
    15301 tccaaacgac aataaccatc ccgccggaat gcctccccgc acacggcggg cggagcattt
    15361 atgagcatcg aacacactcc tcccacacac gacggcgaaa ccggtcaaaa ccatgccgaa
    15421 cgcccttccg ccgatttcga ccgtgtccac tccctctgcg aaatcctcga acctgctttt
    15481 gaacaaatcg aaaacggtac accgcttgaa gacgcgccgc tgcgcgacaa gctgaccgag
    15541 ctgaccgtcc tcttgagcga gctgcaccct gccgacgtgg cggcggtgtt ggaatcgctg
    15601 ccgccgcgcg aacgcaacgt cgtttggctt ctggtcaaac ccgaagacga cggcgaagta
    15661 ttgctggaag tatccgacgc ggtgcgcgaa acactgatcg agtcgatgga taaggacgaa
    15721 ttgttggcgg cagttgacga tttggacgcg gacgaattgg cggaactggc agacgatttg
    15781 ccgcaccaag tggtttatga agccttgcag acgcgcgacg aggaagagcg cgcgcaagtc
    15841 aaagcggcaa tgtcctacga agacaaccaa gtcggtgcga ttatggactt cgagttggtc
    15901 agcatccgcg ccgatgtcgc ctgtgaagtg gtgctgcgtt acctgcgccg cttcgacagc
    15961 ctgcccgacc ataccgacaa gatttttgtg gtcgatgaaa acgatgtgtt gcagggcgtg
    16021 ctgcccatcc gcaaactttt ggtcgccgat cccgaagact tggtggaaaa cgtgatggcg
    16081 aaagatgtcg tgcgtttccg cgccgaagac gacgtggaag aagcggcgca ggcgtttgaa
    16141 cgctacgact tggttaccgc gcccgtcgtc gatgaaaaca aaaagctcat cggtaggatt
    16201 accgttgacg agatggtgga cgtcatccgc gaagaatccg aagccgatat gttgaacatg
    16261 gcaggtttgc aggaagagga agacctgttc gcccccgtat gggattcggt gaaaaaccgc
    16321 tggatgtggc tcgccgtcaa cctctgcacc gccttcctcg ccagccgcgt catcggcgcg
    16381 tttgaaggca gcatcgaaaa aatcgtcgca ctcgccgcgc tgatgcccat cgtcgccggt
    16441 atcggcggca actcgggcaa ccagacgatt accatgattg tccgcgcaat ggcgatgggg
    16501 cagttgacgg gaatgcaggc ggggcgcttg ttgaaaaaag aagtcggcgt tgccctagtc
    16561 aacggcatta tctggggaac cgtcatgggc gcggtttcat ggctgctcta cggcagcctc
    16621 ggcatcgggc tggttatgat tgccgcgatg acgctcaacc tcctgctggc ggcaaccgtc
    16681 ggcgtattaa ttcccgtggt aatggaaaag ttcggacgcg atcccgcact gggcagctcg
    16741 gtgctgatta ccgccgttac cgactccggc ggcttcctga ttttcttggg gctcgccacc
    16801 ctattcctgc tttaaatgcc gtctgaaccc gcgcaaaaat gccgtctgaa gcggaagctg
    16861 cttcagacgg catttgacta tttatccttg ttgcacaaga ttattggacg gtatgccggg
    16921 gcagcccttt ggcaacgccg accacatcct ccccgaacag cgcgttgaca tcggtttcgt
    16981 caaacacata tttgctgtgg cagaaatcgc aatcgacttc gatgctgcct tgttccacca
    17041 ccacgccgcc gacttcttcc ccgcccagca tcaacagcat atcgctgact ttgccgcgcg
    17101 aacaggtgca tgaaaattca aacgtttccg gctcgaacac gcgcggcggc gtttcgtgga
    17161 acaggcggta caaaacatgt tgcgcatcca attctgccag ctcgtccgcc gtcagtgtac
    17221 gcgccagcgt actgacgtgt tcccatgcct cttcatccaa caccgcttca ggcagacgct
    17281 gcaccagcag accgcccgcc gcttcgtcgc ttgcagacag gacgatgtgc gtatcgattt
    17341 gttcggaacg cttcatatag ttcgccaaca tttgcgcgat accgccgcct tccaaaggca
    17401 ctacgccctg ccagggttcg ccgtctttgg gctgcagcgt cagcacgaat acgccgccct
    17461 cgcccaaaag gtcgccgagg ctttcgtcat cggctatttc tgcggtttcg tcccaacgcg
    17521 cggttgcacg gacggtacgg tcggaagccg cttccgcaac cagcattttc agccgccccc
    17581 gcccctgaac ctgcacaatc agcgtgcctt cgtttttgag gttgcccgac agcaacacac
    17641 ccgccgccaa caactcaccc aaagcgcggc ggatggcggc gggatagttt ttctgtttta
    17701 caatgtgctg ccacacgttt tccagacgga cgtgcagccc gcgcacgggc atatcgtcga
    17761 agataaagcg ggtacgcaca tcggcgcggt tgatggcggt ttgattcatg attttctctg
    17821 actgattgtt cggatggcgg ctatatggtt gcggtcggcg cgaaaacaag acggacggcg
    17881 gatgcgcttc ccaaattatc aataaattat ataaaaatca acatattaac tcaatctaac
    17941 aagccgtttt ttgccaaaca gccgtttttt tatatacaat caacaagata ttttcgactg
    18001 atacagcata acatcgcacg gcggcacgat gcctcctgcg cggaaacacc gatatggatt
    18061 cttttttcaa accggcagtt tgggcggttt tgtggctgat gtttgccgtc cgccccgccc
    18121 ttgccgacga gttgaccaac ctgctcagca gccgcgagca gattctcaga cagtttgccg
    18181 aagacgaaca gcccgtttta cccatcaacc gagcccccgc ccggcgggcg ggcaatgccg
    18241 acgaactcat cggcagcgcg atggggctta acgaacagcc cgttttaccc gtcaaccgag
    18301 tccccgcccg gcgggcgggc aatgccgacg aactcatcgg caacgcgatg gggcttaacg
    18361 aacagcccgt tttacccgtc aaccgagccc ccgcccggcg ggcgggcaat gccgacgaac
    18421 tcatcggcaa cgcgatggga cttttgggta ttgcctaccg ctacggcggc acatcggttt
    18481 ctaccggttt tgactgcagc ggcttcatgc agcacatctt caaacgcgcc atgggcatca
    18541 acctgccgcg cacgtcggca gaacaggcac ggatgggtac gccggttgcc cgaagcgaat
    18601 tgcagcccgg agatatggtg tttttccgca cgctcggcgg cagccgcatt tcccatgtcg
    18661 gactttatat cggcaacaac cgcttcatcc acgcgccgcg cacggggaaa aatatcgaaa
    18721 tcaccagcct gagccacaaa tattggagcg gcaaatacgc gttcgcccgc cgggtcaaga
    18781 aaaacgaccc gtcccgcttt ctgaactgat ttcccaagga atacgcaatg agtatgcccg
    18841 aaatacccaa atggtacgac gatgacggac agatcgtgtc ctgtaccgaa aagatcaaag
    18901 tgatgtccga aaatatggcc gagctgtatc agacggcaca agacgcgttt gaagacgcgc
    18961 tgctgatggg ttgcggcgaa cgtcagttgc gcgattacct gctcgcgctg attgaaggtt
    19021 tggaaaatcc ctaccgcaaa gtctgaacac gccccggttg ctgcggcacg gtttatccgt
    19081 gccgtttttg cgtttgtgcg cggcttcggc ttttcagacg gcatatttga cgttatgatt
    19141 aaacagttaa caagatttat cacaacgccg tcaaaagaca gacacacaac atgaacatca
    19201 tccgcgcgct cctcatcatc ctcggctgcc tcgccgccgg cgaaaccgcc gttttcctag
    19261 caggcatcaa actgcccggc agcatcgtcg gcatgggcgt gctgtttgcg cttttgcagg
    19321 caggttgggt caaaacgtct tggctacaac agcttaccga cgcgctgatg gcgaatctga
    19381 cgctgtttct cgtgccgccc tgcgtggcgg tcatcagcta tttggatttg attgccgacg
    19441 attggttttc gatactggtt tccgcctccg ccagcacttt gtgcgtactg ctggttacag
    19501 gcaaggttca ccgctggata cggagcatta tctgatgaac gaaatcctca ggcagcccag
    19561 cgttctgctt ttcctcacgc ttgccgtgta cgcgcttgcg attatcgtgc gcacgcgcac
    19621 gggcaatatc ttctgcaacc ccgtactcgt cagcactatc gtgctgattg cctacctgaa
    19681 aatcctcgac atcgattatg cggtgtacca caacgccgcg caattcattg atttttggct
    19741 gaaacccgcc gtcgtcgtgc ttgccgtgcc gctctaccaa aaccgccgta aaatcttcaa
    19801 ccagtggctg cccgtcatcg tttcacagct tgcgggcagc gttacgggca ttgttacggg
    19861 gatgtatttt gccaaatggc tgggcgcgga acgcgaagtc gtcctctcgc tcgcgtccaa
    19921 atctgttacc aaccccatcg ccattgaaat cacccgctcc atcggcggca ttcccgccat
    19981 taccgccgcc accgtcatca ttgccggtct ggtcggacag attgccggtt acaaaatgct
    20041 gaagaacaca gtcgtcatgc cctcgtccgt gggtatgtcg ctcggcacgg cttcgcacgc
    20101 gatggggatt gccgcctcgc tcgaacgcag ccgccgcatg gcggcatacg cgggactggg
    20161 gctgacgttc aacggcgtac tgaccgcgct gattgcgccg ctgctcatcc ccgttttggg
    20221 attttgaacc cgtttcagac ggcatttcag cccatgctgt ctgaacgccg acacactcgc
    20281 aaggagaacc gttatggctg tcaacctgac cgaaaaaacc gccgaacaac tgcccgacat
    20341 cgacggcatt gccctctaca ccgcccaagc aggcgtgaag aagcccgggc ataccgacct
    20401 gacactgatt gccgtagccg ccggcagcac cgtcggtgca gtcttcacga ccaaccgttt
    20461 ctgtgccgcg cccgtccaca tcgccaaatc gcaccttttc gacgaagacg gcgtgcgcgc
    20521 cctcgtcatc aacacgggca acgccaacgc gggtacgggc gcacagggca gaatcgatgc
    20581 tttggcagtg tgtgccgccg ccgcccggca aatcggctgc aaaccgaacc aggtgctgcc
    20641 cttctccacc ggcgtgattc tcgaaccgct gcccgcagac aaaatcatcg ccgcgctgcc
    20701 caaaatgcag cctgccttct ggaacgaagc ggcacgcgcc atcatgacca ccgacaccgt
    20761 gcccaaagcc gcctcgcgcg aaggcaaggt cggcgacaaa cacaccgtcc gcgccacggg
    20821 catcgccaaa ggctcgggca tgattcatcc caatatggcg accatgctcg gtttcatcgc
    20881 caccgatgcc aaagtttccc aacccgtcct ccaactgatg acgcaggaaa tcgccgacga
    20941 aaccttcaac accatcaccg ttgacggcga caccagcacc aacgacagct tcgtcatcat
    21001 cgccaccggc aaaaacagcc aaagcgaaat cgacaacatc gccgacccgc gttacgccca
    21061 actcaaagaa ttgttgtgca gcctcgcgct cgaactcgcc caagccatcg tccgcgacgg
    21121 cgaaggtgcg accaagttca tcaccgtccg cgtcgaaaac gccaaaaccc gcgacgaagc
    21181 ccgccaagcc gcctacgccg tggcacgttc gccgctggtc aaaaccgcct ttttcgcctc
    21241 cgaccccaac ctcggcaggc tgctcgccgc catcggttat gccggcgttg tcgacctcga
    21301 tactgacctc gtggaaatgt atctcgacga tattttggtt gccgaacacg gtggacgcgc
    21361 cgcaagctac accgaagcac aagggcaggc ggtgatgtcg aaggccgaaa tcaccgtccg
    21421 catcaagctg catcgcggac aaagtgccgc caccgtctat acctgcgacc tgtcgcacgg
    21481 atacgtttcc atcaacgccg actaccgttc ctgacccgac acggcttcag acggcataca
    21541 taaaatgccg tctgaaccgc cggacaacat gccatgacct ccacattccc ccgccgcctc
    21601 gcccgcaaaa tccgccaaac ccgccgcctg tcgcgcaaaa gcatcgcctt tctgttcctt
    21661 ttggcaggtt cggcactcgt cgccctgacc gcgctgtttt ttgcccatct tgccgatttt
    21721 gcgctggaac tgaacgccaa actggttcaa caatacccgt ggttcgcgtg ggtcgcgctt
    21781 cctttgggtt tgccgcttat tgcgtggctc acacgcaaat tcgccccctt caccgccggc
    21841 agcggcatcc cgcaggtcat cgcctcactg tcgctgccct acggcgcaca gaaaacgcgg
    21901 ctgatccgcc tcgggcagac gctgctgaag attccgctga cctttttggg tatgctgttc
    21961 ggcgtgtcca tcggacgcga aggtccgtcc gtgcaggtcg gcgcggcagt gatgggcgcg
    22021 tggggcgcgt ggtgcaagaa acacggcttg gcattcaaag ggatgcagga aaacgatttg
    22081 atggcggcgg gcgcggcggg cggtttggca gccgcgttca acgcgccgct ggcgggcgtg
    22141 attttcgcca ttgaggaact cgggcgcggc atcatgttgc gctgggagag gcaaattctt
    22201 ttgggcgtgc tcgcctccgg tttcatacag gtcgccattc agggcaacaa cccgtatttt
    22261 tccggcttca acggcggcgt attggaacat atctttctgt gggtcgcact gtccggcctg
    22321 gtttgcggcg cggcgggcgg cctgttcgga cgtttgctct atcgcggtgc ggcggcgttt
    22381 gcaccgcgca agatacgcgg cttcatccgc aaccgtccgc tgctgctggc ggcactgatg
    22441 gggctgctgc tcgccctgct cggcacgttc taccaaggca aaacctacgg cactggctac
    22501 cacgaagccg cccaagccct gcacggcatc tacgaagccc ccttcggact cgccgccgcc
    22561 aaatggctcg ccaccgtatt cagctattgg gcaggcattc cgggcggcat tttcactccc
    22621 tcgctgacca taggcgcggt tttgggcgag catatcgccg ccatcgccga catatcgcag
    22681 ggtgcaaaca tcatcgtcct catctgcatg gcggcatttc tggcgggcgc gacacaatcc
    22741 ccgattactt ccgccgtcgt cgtcatggaa atgacgggcg gacaaagcct gctgttttgg
    22801 atgctaattg cctgcatttt cgcctcgcag gtttcgcgcc agttttcgcc gcgtccgttc
    22861 taccacgcat cgggaatgcg cttccgccag cgcgtgcttc aagaaaccgc cgcccaaacc
    22921 ggcaatgcgc ccgcaagacc gcaaacagca aacagcaaaa cgggaatgcc gtctgaaaat
    22981 taaaacgccc ccgatcaaac gccggcagcc gccttgattt gaataccgtt ccgccgccgc
    23041 ttgaaatttc agcaacaatg ccgtctgaac gacagaatgc ggttttcaga cggcatttcc
    23101 ccatcccgat attgcctaaa caaaaccgaa gcgtttgcta taattctatt ttttaccgca
    23161 cacgcaccaa tcatgtttcc cgatttctcc caaaccctct ccaaagaccg ccacttccta
    23221 cgttccgcct tcaaaaatcc caacaaatac ggcggcttgt ccaaagtcga agaaaaatac
    23281 caaaaatcgc acgaaatctt tttgaagcgt ttggctgcct tgcccaaacc cgaattcgat
    23341 aacaccctgc ccgttcacga gaagctcgac gaaatcaaaa aagccattgc cgagaatcag
    23401 gtaacgatta tttgcggcga aaccggttcg ggcaagacca ctcagttgcc caagatttgt
    23461 ctggaactcg ggcgcggggc ggcgggtttg atcgggcata cccagccgcg ccgtttggcg
    23521 gcgcgttccg tagcagagcg gattgccgaa gagctgaaat ccgaaatcgg cagcgcggtc
    23581 ggctataaag tgcgcttcac cgaccacacc tcgcgcgatg cctgcgtcaa gctgatgacc
    23641 gacggcatcc tgctggcgga aacccagacc gaccgttatc tcgccgccta cgacacgatt
    23701 atcatcgacg aagcgcacga gcgcagcctg aacatcgact tccttttggg ctatttgaaa
    23761 caactcctgc cgcgccgccc cgatttgaaa gtcatcatca cctcggcaac gatagacgca
    23821 gaacgctttt cccaacactt caacggcgcg cccgtgctgg aagtgagcgg acgcacctat
    23881 cccgtcgaaa tcctctaccg accgctgatc ggcaaagacg aagacgacgc ggaaatagaa
    23941 ttgaccgacg cgattgtcga tgcggcggac gaattggcgc gatatggcga aggcgatatt
    24001 ttggtattcc tgccgggcga acgcgaaatc cgcaaagccg ccgaagccct gcgcaaatcc
    24061 acgctacgcc gcaacgacga aatcctgccc ctgttcgcac gcctgtcgca cgccgagcag
    24121 cacaaaatct ttcacccctc cggagcgaaa cgccgcatcg tgctggcaac caacgtcgcc
    24181 gaaacctcgc ttaccgtgcc gggcatcaaa tacgtcatcg acaccggcct cgcacgcgtc
    24241 aaacgctatt ccgcacgggc gaaagtggag cagcttcata tcgaaaaaat ctcccaagcc
    24301 gccgcccgcc aacgctccgg ccgctgcgga cgcgtctccg caggcgtatg tatccgattg
    24361 ttttcagaag aaaatttcaa cagccgcccc gaatttaccg accccgaaat cgtccgcagc
    24421 aacctcgccg ccgtcatcct gcgcatggca tcgctgaaac tcggcgacgt agcggcattc
    24481 ccatttttag aaatgcccga ttcgaggtat attaatgatg ggtttcaggt gttgctggaa
    24541 ttaggggcag tggaggccgc ctgaaaacaa gtaggatata cacttctacc tgacaaagac
    24601 caacaaacga taaaactacg gcacatctag atgcccaacc tacaacattg cacacggtaa
    24661 aaacatgaaa cacttactca tcgattttga aaacgtccag ccgcaaaact tagacaaatt
    24721 accaaccgaa aatacccata tttggctatt tataggtgta ttacacaaaa tgttacctat
    24781 tagtctggtg caatccctac tacgtttcgg cgaacgtgtc catcttgtcc agttacaaaa
    24841 aacggggaaa aacgcattgg atttttacct gtcctattac ctcggacaaa ttaccgccac
    24901 agaccccaat gcccaaatcg gcatactctc gcgtgatgga ggatacgatg ttctggtcga
    24961 acatattttg aaaaaccacc aggcgaaggg tatcgtgcgc ctagccaata tagatgaagt
    25021 acaacatcag aaaattacta ccgaaccgcc gtcagcattg ctggaaaaca ctcctcagcc
    25081 tgaaaccacc ctcaaaccac agcaaccatt aacttcctat ttccaagcag ccctaactgc
    25141 actgcgccgc cccgacgctt tccgcccctg ccgcctgcat aacctgcgac aaaatctgcg
    25201 taagcatatt ttgagtgatt tgtttaaaga aaaaaccgat gaagaatgcg aaataaccac
    25261 tgctaacgtt atcaataaac tcaaagcaca aaacttcatc agcattgatg aacaggaaac
    25321 cgtttcctac catctcagtg ataatgattt gttacaaaga atccaacgcc atattttaag
    25381 ccaacgtccc aaaacctacg ctgattttca agccgtcgtg caaaaccgag cagatgcact
    25441 tcacttaaca gtcggtacca acgacattca atcctttgcg cgacatttgc gcgaccaaaa
    25501 cctgatccgc caaaacaatg ggaaaattga atatgcaccg tttactgaac ctaaaccaca
    25561 gccaacgccc aagcagccta aaaaaaccgc atgggaacct gatgaaatta tttggaaaaa
    25621 agtgattgcc gcgttatcgt taaagaaccg tcctaataaa accaaaactt tacgcaatac
    25681 aatccaggca ctcacaaaat ccaatgcaca agaaactgac aaactgctac aacatttaca
    25741 agatacccaa gtcctacgta ttgacggtac taaaattgtg tatatcaagt aggatgggca
    25801 tttatgcctg acctacaaaa ccaaaaacaa cctaccaccc ttaatcaact ccacaaaccc
    25861 ttttcagaca acctcgtttt ttgaaaaaca atctgtaaac agatagctgc tgaagaatac
    25921 cgttgccgag ccccaaaacc cgtcctgcaa cttttattgt gaacttccca ttatgaaaaa
    25981 atcccttttc gttctctttc tgtattcgtc cctacttact gccagcgaaa ttgcttatcg
    26041 ctttgtattc ggaattgaaa ccttaccggc tgcaaaaatg gcagaaacgt ttgcgctgac
    26101 atttgtgatt gctgcgctgt atctgtttgc gcgttataag gcgacgcgtt tgttgattgc
    26161 ggtgtttttc gcgttcagca ttattgccaa caatgtgcat tacgcggttt atcaaagttg
    26221 gatgacgggc atcaattatt ggctgatgct gaaagagatt accgaagttg gcggcgcggg
    26281 ggcgtcgatg ttggataagt tgtggctgcc tgcgttgtgg ggcatgttgg aagtcatgtt
    26341 gttttgcagc cttgccaagt tccgccgtaa gacgcatttt tctgccgata tactgtttgc
    26401 cttcctaatg ctgatgattt tcgtgcgttc gttcgacacg aaacaagagc acggtatttc
    26461 gcccaaaccg acatacagcc gtatcaaagc caattatttc agcttcggtt attttgtcgg
    26521 acgcgtattg ccgtatcagt tgtttgattt aagcaaaatc cctgtgttca aacagtctgc
    26581 gccaagcaaa atcgggcagg gcagtgttca aaatatcgtc ctgattatgg gcgaaagcga
    26641 aagcgcggcg catttgaaat tgtttggcta cgggcgcgaa acttcgccgt ttttgaccca
    26701 gctttcgcaa gccgatttta agccgattgt gaaacaaagt tattccgcag gctttatgac
    26761 ggcagtatcc ctgcccagtt tctttaacgt cataccgcat gccaacggct tggaacaaat
    26821 cagcggcggc gataccaata tgttccgcct cgccaaagag cagggctatg agacgtattt
    26881 ttacagtgct caggctgaaa accaaatggc aattttgaac ttaatcggta agaaatggat
    26941 agaccatctg attcagccga cgcagcttgg ctacggcaac ggcgacaata tgcccgatga
    27001 gaagctgctg ccgctgttcg acaaaatcaa tttgcagcag ggcaggcatt ttatcgtgtt
    27061 gcaccaacgc ggttcgcacg ccccatacgg cgcattgttg cagcctcaag ataaagtatt
    27121 cggcgaagcc gatattgtgg ataagtacga caacaccatc cacaaaaccg accaaatgat
    27181 tcaaaccgta ttcgagcagc tgcaaaagca gcctgacggc aactggctgt ttgcctatac
    27241 ctccgatcat ggccagtatg ttcgccaaga tatctacaat caaggcacgg tgcagcccga
    27301 cagctatctc gtgccgctgg tgttgtacag cccggataag gccgtgcaac aggctgccaa
    27361 ccaggctttt gcgccttgcg agattgcctt ccatcagcag ctttcaacgt tcctgattca
    27421 cacgttgggc tacgatatgc cggtttcagg ttgtagcgaa ggctcagtaa cgggcaatct
    27481 gattacgggc gatgcaggca gcttgaacat tcacgacggc aaggcggaat atgtttatcc
    27541 gcaataagcg gcgtgaaaac caataaagac aaatttagat gatgtcgggg aagatgcccg
    27601 accgacaaga ctatgcaaaa tatgaaaaac caaatacgcg gatcaggcat ggatgcacga
    27661 tccaacccgg ccaatgtttc agacggcctg caaaacagtt cgggccatat cggtaccaac
    27721 acgcgctacc gtctgaccaa actcggcgaa caaatggcgc gcctgcccat cgacccgaaa
    27781 atcgcgcgca ttttgttggc ggcgaagaag cacgactgca tggcggaaat attggtgatt
    27841 gcgtccgcgc tatcgattca agacccgcgc gagcggccgc tggaggcacg cgatgccgcc
    27901 gccaaggcgc atgaacgctt taccgacaag caatccgatt tccttgccta cctgaatatt
    27961 tgggacagtt tccagcgcga gcgcgacaaa ggtttgtcca acaagcagct ggtgcaatgg
    28021 tgccgccaat atttcctgtc gcacctgcgg atgcgcgagt ggcgcgagct gcaccaccag
    28081 cttgcccaaa ccgcgattga aatgggtttg accaccaagg aagcggcttt cagacgactt
    28141 tccgaaatca agcagcttac gtcgtctgaa aaccaaggcg atcaagactt atccgccaaa
    28201 cgcaaacaaa aacaactgga taagaaacaa caccgcgccc aaatccgcgc cgccaaagaa
    28261 gcgggctacg aacaaatcca ccgcgccctg ctcaccggcc ttatcgccaa cgtcggcatg
    28321 aaatcgcccg acggcaacga ctataccggc gcgcgcggca gccgtttcca ccttttcccc
    28381 gcctccgccc tgttcaaagc caaacccaaa tgggtgatgg cggcagaact cgttgaaacg
    28441 acaaaacttt atgcgcgcga cgtcgccgtt atccagcccg aatggattga gcaggaagcc
    28501 ccgcacctcg tccgctacca ctatttcgag ccgcactggg aacaaaaacg cggcgaagtc
    28561 gtcgccagcg aacgcgtgac gctttacggc ctgaccgtat tgccgcgccg ccccgtgcct
    28621 tacggcaaag ttgcgcccga agaagcacgt gaaatcttta tccgcagcgc gttggtggcg
    28681 caggaatgcg atttgaaagc ggattttttt gtccacaata aaaagctgat taaagaaatt
    28741 accgaactcg aacacaaatc gcgcaagcaa gacgtattgg tcgacgacga agccctgttt
    28801 gcgttttatc acgaacggct gcccgatttt tatacggcgg atgcggtttc agacggtctg
    28861 cgtcctgcaa atccccagca aactgccccc tcctacgcga gggaagaacg gagagagggc
    28921 aaaacagttg ccgcacaaac caacttctcc gcaactgcag caaaccctct cccgcaggag
    28981 agggaacaga gtgcctcagc ttcaacgttt tcagacgacc tgcgtcctgc aaatctgcag
    29041 caaaccgccc cctcccccgt gggggagggc tggggagagg gcaaaacagt tgccacacaa
    29101 accaactttt ccgcaacctc aacaaaccct ctcccgcagg agagggaaca gagtgcctca
    29161 gcttcaacgt tttcagacga cctgcgtcct gcaaatctgc agcaaccctc tccctccccc
    29221 gtgggggagg gctggggaga gggcaaaaca gttgccaccc aaaccaactt ctccgcaacc
    29281 acagcaaacc ctctccctaa ccctctccca caggaggggg aacagagtgc cgcagcttca
    29341 gcggtttcaa acgaccctca accccaaaag cagccagcac cccaaaaaga ccgtctgaaa
    29401 cccctccccc tcgccgacat ccgcaccttc caagcctggc tcaaaaccgc cgaacgcgag
    29461 aacccgcgcc tgctgttcct cagccgcgac gatttgatgc aacacgccgc cgcacacatt
    29521 accgaagaac aattccccaa attctggcaa accgcagacg gcaagttcga actcagctac
    29581 cgcttcgagc cgcaccaccc gctcgacggc gttaccctca ccctaccgct gaccgtcctc
    29641 aaccgcctgc acgcgccgtc gctcgaatgg ctggtgcccg gtatgttgcg cgaaaaaatc
    29701 cagctgctga ttaaagctct gccgaagcaa atccgccgca tttgcgtgcc cgtacccgat
    29761 ttcatcaccc aatttttaag ccaaaaccct gatcgcaacg cccccatcct gccccaactt
    29821 gcccaagcca tcgccaaaac cgcaggcgac atccgcatac tcgagcaaat caaccaagac
    29881 gaatgggccg cgttcaggct gcccgaacac tgctatttca acctccgcat tatcgacgac
    29941 ggcggacaag agcttgccgg cggccgcaaa ctgcatgaat tgcaacaaca gctcggccaa
    30001 gccgccgccg ttaccttccg cgacaacacc caagaattcg agcgcgacaa cgtgaccaca
    30061 tgggacattg gcaccctgcc cgaatccatc aaattcgcac gcggcaaaca acagctcacc
    30121 ggctacctcg gtctgcaaaa agaaaaagac ggccgcatcg ccctgcgcct gtgtgacacc
    30181 accgaagctg ccgaacaggc acaccgtcaa ggcgtcatcg aattgatgaa gctgcaatta
    30241 aaagagcagg taaaagacct gaacaaaggc atccaaggct ttacccaagc cgccatgctg
    30301 ctcaaacaca tcaacgccga caccctgcgc gacgacctca cccaagccgt ttgtgaccgc
    30361 gcctttatcg gcgaagacga gctgccgcgc aacgaaaaag ccttcaaaga acaaatcaaa
    30421 cgcgcccgca gccgcctgcc cgccgtcaaa gaagccctca gccgctacct gcaggaaacc
    30481 gccgccgcct acgccgaact gaacggcaaa ctcggcaaac acccattgac ccaccttcta
    30541 agactacgcc tgcaaaccct gctcgccgcc ggcttcgcca cccgcacccc gtgggcacaa
    30601 tggccgcgcc tccccatcta cctcaaagcc atgaccctgc gcctcgaaaa atacagcagc
    30661 aaccccgccc gcgacgcagc ccgcgaagcc gatatacagg aattggaaca aatgtggcag
    30721 gaaaaaaacg acggtttggt gaaacaagga ctacccgttt cagacgacct caccgcgttt
    30781 aaatggatga tcgaagaatt gagggtgtcg ctgttcgcgc aggaattgaa gacaccgtat
    30841 ccggtgtcgg tgaagcggct gttgaaagag tgggaggatc ttaattaaag gaagatgccg
    30901 tctaattttt tcagacggca tcttttattt ccctgaccaa ccgatttaat cgctccctac
    30961 aaatagcttt attttttaat tcgcactccc aaacgacaat cactttgaaa ccgatctttt
    31021 ccagctcggt ttcattttta atatcacgtt cgcaattttt tgtgattttt tccaaccaaa
    31081 aatccgtatt gcttttcggg atatgtcctt tattacaaga atgcccgtac caaaaacagc
    31141 cgtgtataaa cacgactgtt ttatatttcg acaaaacgat atccggtttg cctacatagc
    31201 gtttatcgtt tttccgataa cggaaacctt ggaaaaaaag aaatttcctt accaatactt
    31261 ccggtttggt ttctttgcta tggatttttg acatcacgaa agaacgtttg gatgtagtga
    31321 aaatatcggt cataaattac ttggaagata ccaccttcga tcgttttttt gtctgttcca
    31381 cttttcttaa caaatacgaa accaagctta ttaaatccaa agcatcctta tcattaaaaa
    31441 tactaggaga taaatttgct cttaactcat gttgtacagg atttctaaat ccagtccatg
    31501 ttccgcatgc aagctgttca agaccatctt ctaaattttg ttccgccttc gttttgttat
    31561 tcgttaatag aagtatagaa tttttactcc cgaaagcttg ttcaatcaat tctcttccgt
    31621 ctatgctatg aagacctgtt tgcttctgaa ccttatcttc gaaaacttta actgcctcga
    31681 ttgcagcact taaataatcc ttcttttcat tgtaaaaatc attacaggca gtatgcaaat
    31741 cctgatgcaa atgccgccaa tgatagtaag gatattctgg aataatacta tgtaaggcag
    31801 agattacttc cgcttgctct ttatttgtaa gctccgaatc ttccaaacga ttcaaaataa
    31861 cttctactgg ttccctttcc ttttctggca atttgttttt ccaatcttcg atatcaactc
    31921 cgctttcgcc tttcacttcg tcatctttct ctttttttct tctttttctc caatcttgcc
    31981 cgatggctcg gacgatctcc ccaaggaaaa cacgtaattt tgaggttttt gaatgcttcc
    32041 agtctaatga tgtcctgtcg gtagcaatta aatcttcatc gtcattatct ataaagtcca
    32101 cattcagcca tccagtcaaa tatgaataga aatggctgga ttcgctatca gtgaaaaact
    32161 cgggcatatt taccattctg ccgttggcaa acaaggtaat acctttcata ttattcttta
    32221 aaggtttttc cgttgtaatg aacttacctt gtattaatcc gctgtattca tactgttcga
    32281 attttgaaga taacgatgaa atattggttg ctaaatcctg gtattcccat tcgaattgtg
    32341 gggtaacaag atcatatttt agatttccat caatgatttt aggttcagag ccgttcaagc
    32401 ttactttgat tttaaaatcc tgagccggaa aatcaaacaa gcgggaaaga tgctctacat
    32461 aattatctaa cggatatcct tgctttttcg tcagttcggt taaagttatg gttgtcccgc
    32521 tttcgatatt ggattcaaca gattcttttc gaaactccgg ttgataaata cccttgcttc
    32581 ttcgaatctc tgcataatcc aaagtaaaag taaccctttc gtttccctgg atagtagaaa
    32641 tttcaatttt gttgccaagc ccgaataatg ccaatttacc aaggcctttt ttacccgttg
    32701 gaattcttcc gcacggggag gcttgttttt cttcccttct gttccgaccg attctcaaat
    32761 aaaaatcatt gatttcatcg aagctcattc ctataccatt atctttaata acgattttat
    32821 gctccggttt atcgaataac ctaacttcca cttccgtagc acatgcatcg taggcattcg
    32881 ctatcaactc ggcaatcgca ggaacagtgt gcgaatacat ctttacaccc aaatgttcga
    32941 ttacggttgg ctcgaaactc attttcaatt tttcttcaga catgttttct ccttttctaa
    33001 cattcgatat tatcaacaat tgccctgcct attttttccg catacatcgg aggaacggca
    33061 ttaccaatca aacgggctat cttgtccctg ctgcccgctt taaagacata gttacgaggg
    33121 aacgattgca atgttgcacc ttcgcgcagt gataacgccc tatcttcttc aggatgggca
    33181 aacctcccgt tggaaatact aaagaatttg gtcgttatgg taggtgcggg cttatcccac
    33241 catagcctac caaacgtgtc tttaaaacta ttgtcttttc cgataaaaca ttccagctgt
    33301 aattcgggaa tatgcgcaaa agccaaacgg tttcctccgt ttttaggtat caaagccaat
    33361 cgtttcaagt tgatatcaga taatcccgca cagctatgca taaaatccgt ttcgtcttgg
    33421 tgtcctgcaa taattttggg aaagccattt tccattccca aaacatcgcg taccgtaagc
    33481 cgtttgcccg aatacttgac tggttccagc ttttctttgg ttattctgtt tgcaattaac
    33541 gtaaatcttt tacggctttg gggaattccg taatcagccg tattgtgaat accgaaatgg
    33601 acggtaaatc ctttttcttc caataacttt atgaaattat caagcctgct ttctttcatt
    33661 ctgctcagaa ttcccggtac gttctctaca acaacatatc cgggattgaa atattctaca
    33721 aaccgctgaa actccaaaag caaactcttg gatttttccg attttctttt gtctgtttga
    33781 ataacactcc aatattgaca aggactacaa cctataagaa tcaaatcatc atcattcttt
    33841 tttaacccga gttctttttc caaggtttca ggctgcaatt caaaaacatc agcctgtata
    33901 aatttggcat ttatattggc ttcatatgtt tctttgcagc tgggttcgta atctatcccg
    33961 gctaaaacct gaataccggc ggattgcatt ccatagctca ttccacctcc gccacagaaa
    34021 aaatcaaccg cctttaactt actgtttttc atttctattt aaataattag actagtgaga
    34081 actaacttta tataacatat aatttattgc ttatcaaaaa taatttgaga ttttattaaa
    34141 attcatgcat ctaaataagc agaacttaat ttcctccaaa cgagcggatg tcttcaaatg
    34201 cacacttcac agaaaggtcg tctgaaaccc attttcagac gacctttttc cacttcccaa
    34261 aacaagaatt cccaccccct ccccaaacgt cgcccgaacc agtaaacttc tcatatttca
    34321 acaactcctt ggaagcaaac catgtctggt atctacctac cccgcctatt cccgccccat
    34381 atcgccgaac gcggcctgtt gtattttcag cagggcaagg ttctcgatgt ccgaaaaact
    34441 tccgccgggc attatcgggc ggaggtgtgc ggttcggaaa actattgggt atagttgaag
    34501 ctggatagtg atttgtatat taaagacgaa ggctgcaatt gtccttatat ctaagagtgc
    34561 aaacatacct taaattacta tattgcatag gcaaaataca agcctataac gaattggaaa
    34621 caaaatgccg tctgaaaaca tcttcagacg gcattataaa atctgttcac cttttcagat
    34681 gagtaatgta cacccctata caatttttgc tactataccc cataaatcca cggctaaaga
    34741 tatccttatt atgtcctatg atttatcgaa acgacttgta atcggcttag catcaagtgc
    34801 cctattcgac ttatccgaat cggataatat atttagaatg gaaggggcag aaacctatag
    34861 gcaatatcag agagaaaaac aaaaccatcc cctaaaaaag gcgttgtctt tccatttatt
    34921 aaaaaacttc tgtcaatcaa tgaaataaac ccaaacgacc caacgattgg gtttattctt
    34981 ttatccagaa acaatccaga tacagattac gagtcataac tataggctta atattacacg
    35041 attttcattc catcaaggcg gaaaaccgca caaatactga aacactatcg atcgatttgt
    35101 aaacaagcct acttaagtaa cttgcagtcc ttatcatttc ctttaaaata atccagcccg
    35161 tcactacacg aactggcgga cttcttgcaa ataaaggtta ctagattttc attcatctta
    35221 ataataaaag gatttttatc tttatctatg gctaccgcct tcaacatgaa tttactgtct
    35281 aaggccccgc gcgcgattcc attcaaacgg atacaaaagc cttctgcctc tttaatcggc
    35341 aaacttggcc atttggtaga tgtttgttta aatctcccat tctgcagata aaacttttcc
    35401 ataaaatgtg cattttctaa caaggctgcc cgcactgtat ttatctttgc tttctcaaca
    35461 taattgcgat agctcggata aacaattaaa gcaagtacag acaatatcaa gaccactgat
    35521 attaattcaa ccagcgtaaa cccccgatta tcagtcatta ctttacttcc aataagaaca
    35581 gattattcaa catatttctt tgaacagact tactatccca ttcagcagta tgcatatttc
    35641 ccactctatt ttttatccgc gggcagcact ggtttagctg ggccttttgg tgcgggcgca
    35701 ccgacagaag cctgatcctt cagcttcgcc agcaccgcag ggccgatgcc ctttaccttg
    35761 gtcaaatcgt ctacagactt gaacgcaccg ttttgcgcac ggtattccgc aatggccttc
    35821 gccttcgcag ggcctatgcc cggcagcgcc tccagctcct gcagcgaagc cgcattgatg
    35881 tttaccgccg caagggagaa ggcgcaggag aacagcatac agaacagcac gaacattttc
    35941 ttcatggttt ttcctttaag ggttgcaaac aataaaccgc atcttgcgac gataaaacgg
    36001 gtcattctaa aatgaatatc ccaaagtttc aagccgttcc tccgcaaacc cgaccggacg
    36061 ccgtacggat gccgtcccgc catc
//
LOCUS       DAWFJR010000012        35404 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWFJR010000012 DAWFJR010000000
VERSION     DAWFJR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35404
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.012"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(361..636)
                     /locus_tag="WGR04_000497"
     CDS             complement(361..636)
                     /locus_tag="WGR04_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0794115.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(633..1916)
                     /locus_tag="WGR04_000498"
     CDS             complement(633..1916)
                     /locus_tag="WGR04_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0794116.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(2069..3487)
                     /gene="glnA"
                     /locus_tag="WGR04_000499"
     CDS             complement(2069..3487)
                     /gene="glnA"
                     /locus_tag="WGR04_000499"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ0794117.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPTVKGGYAPVAPIDCGQDLRSAMVNILEGLGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            3511..3720
                     /locus_tag="WGR04_000500"
     CDS             3511..3720
                     /locus_tag="WGR04_000500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794118.1"
                     /translation="MAKYAERGRLAQSQHDSTTSMPSETGPASNSKLENIVSDGKIVN
                     NMLRFVCSKAEYPPFYPNADKTDAV"
     gene            complement(3761..4138)
                     /locus_tag="WGR04_000501"
     CDS             complement(3761..4138)
                     /locus_tag="WGR04_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794119.1"
                     /translation="MKKMLCLAVFALGLATPAVAADWVLVDSNTATFGDADSRTDNSA
                     WFETRFAKPQKIGNGKFFNTVRALQEMDCSGKRRRILTITWYSKSGNSIASYTPSYAE
                     WNYVIPGTLGEAQYKFVCNGYPR"
     gene            4310..5119
                     /gene="aroE"
                     /locus_tag="WGR04_000502"
     CDS             4310..5119
                     /gene="aroE"
                     /locus_tag="WGR04_000502"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ0794120.1"
                     /translation="MTALPRYAVFGNPVAHSKSPQIHRQFALQEGVDIEYGRICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFALADEHSDRALAAGAVNTLILLKNGKLRGD
                     NTDGIGLTNDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTYAKA
                     EELARLFGIEAVPMADLNGGFDIIINGTSGGLSGQLPAVSPEIFRGCRLAYDMVYGDA
                     AQAFLNFAQSNGAAEVSDGLGMLVGQAAASYSLWRGFTPDIRPVIEYMKAL"
     gene            5122..5823
                     /gene="mtgA"
                     /locus_tag="WGR04_000503"
     CDS             5122..5823
                     /gene="mtgA"
                     /locus_tag="WGR04_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ0794121.1"
                     /translation="MFRIIKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWMPYKRISTNLKKALIASEDARFAGHGGFDWGGIQNAIRRNQ
                     NSGEVKAGGSTISQQLAKNLFLNESRSYLRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYKKPAADLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(6148..6223)
                     /locus_tag="WGR04_000504"
     tRNA            complement(6148..6223)
                     /locus_tag="WGR04_000504"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6188..6190),aa:Phe,seq:gaa)
     gene            complement(6414..7148)
                     /gene="lptB"
                     /locus_tag="WGR04_000505"
     CDS             complement(6414..7148)
                     /gene="lptB"
                     /locus_tag="WGR04_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ0794122.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVMLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEISTKDKSRIDGEVEKLLADLNIERLRNNPAPSLSGGERRRVEIARVLAMKPR
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(7222..7752)
                     /gene="lptA"
                     /locus_tag="WGR04_000506"
     CDS             complement(7222..7752)
                     /gene="lptA"
                     /locus_tag="WGR04_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ0794123.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLNQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRNDKGEQFMKADGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVIIQPSSTQKSE"
     gene            complement(7733..8314)
                     /gene="lptC"
                     /locus_tag="WGR04_000507"
     CDS             complement(7733..8314)
                     /gene="lptC"
                     /locus_tag="WGR04_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ0794124.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVKTEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGLLNFSSKVKATIYDTKDM"
     gene            complement(8311..8847)
                     /locus_tag="WGR04_000508"
     CDS             complement(8311..8847)
                     /locus_tag="WGR04_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0794125.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVIVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
     gene            complement(8912..9886)
                     /locus_tag="WGR04_000509"
     CDS             complement(8912..9886)
                     /locus_tag="WGR04_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ0794126.1"
                     /translation="MAENGKYLDWAREVFHTEAEGLREIAADLDENFALAADALLHCK
                     GRVVIMGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEITAVIPALKRKNITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQCRLKGVFTDGDLRRLFQECDNFTGLSIEEVMHTHP
                     KTIPAERLATEALKVMQANHVNGLLVTDADGVLTGALNMHDLLAARIV"
     gene            9982..11037
                     /gene="tal"
                     /locus_tag="WGR04_000510"
     CDS             9982..11037
                     /gene="tal"
                     /locus_tag="WGR04_000510"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ0794127.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLSPKQRYETMAVADVRAACGICLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRVQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKAAYQDWEQYFTTPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLATRLQED
                     GLKQFEEAFEKLLAPLA"
     gene            11281..11640
                     /locus_tag="WGR04_000511"
     CDS             11281..11640
                     /locus_tag="WGR04_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0794128.1"
                     /translation="MINLELKAINLYDCAFEDFVPEIKDNFCVGIDLDIGTDDSDAAD
                     IFSVRICSPKWILHNCFQNQRVKWGAGMMIMNEFNHSVIKSEIEKILKECSKETWEKS
                     LTYLLRFFSWEFEDYQC"
     gene            11714..11911
                     /locus_tag="WGR04_000512"
     CDS             11714..11911
                     /locus_tag="WGR04_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794129.1"
                     /translation="MTKLVSRNASNERIADRAFDFFSPALMDGSATEEQYNALYDLTL
                     LEEPGMELNKDEIMALINSLK"
     gene            11928..12815
                     /gene="gluQRS"
                     /locus_tag="WGR04_000513"
     CDS             11928..12815
                     /gene="gluQRS"
                     /locus_tag="WGR04_000513"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ0794130.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARAHGGKWLIRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQAGARRGADGFVYNGRCRHPGQRPALQGKQPAWRIRVPDRIIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLDVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAVTAP"
     gene            complement(12885..13895)
                     /gene="dusA"
                     /locus_tag="WGR04_000514"
     CDS             complement(12885..13895)
                     /gene="dusA"
                     /locus_tag="WGR04_000514"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ0794131.1"
                     /translation="MNDNTQTLPPRRLSVAPMLDWTDRHYRYLARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVKIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANM
                     RE"
     gene            complement(13926..14417)
                     /locus_tag="WGR04_000515"
     CDS             complement(13926..14417)
                     /locus_tag="WGR04_000515"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ0794132.1"
                     /translation="MRAVIQKTVGAKVDVVSENGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(14491..15249)
                     /locus_tag="WGR04_000516"
     CDS             complement(14491..15249)
                     /locus_tag="WGR04_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0794133.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQEQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            complement(15320..16186)
                     /locus_tag="WGR04_000517"
     CDS             complement(15320..16186)
                     /locus_tag="WGR04_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0794134.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(16235..16513)
                     /locus_tag="WGR04_000518"
     CDS             complement(16235..16513)
                     /locus_tag="WGR04_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0794135.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(16513..16803)
                     /locus_tag="WGR04_000519"
     CDS             complement(16513..16803)
                     /locus_tag="WGR04_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ0794136.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(16806..17336)
                     /locus_tag="WGR04_000520"
     CDS             complement(16806..17336)
                     /locus_tag="WGR04_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ0794137.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            17644..20319
                     /locus_tag="WGR04_000521"
     CDS             17644..20319
                     /locus_tag="WGR04_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0794138.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTV
                     AEVQTPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENITETV
                     AETPDFNATADDLSALLQPSEVPAVEENAAETVADDLSALLQPAEAPAVEENVTKTVA
                     EIPDFNATADDLSALLQPSEVPAVEENVTETVAETSDFHTAADDLSALLQPFETPAVE
                     KFEAPAIEEKAAETVDKTPDFHTVADDLSALLQPAEVPAVEENAAEITLETPDSNTSE
                     ADALPDFLKDGEEETVDWSIYLSEENIPNNADTGFPSESVGSDAPSEAKYDLAEMYLE
                     IGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            complement(20492..20968)
                     /gene="gloA"
                     /locus_tag="WGR04_000522"
     CDS             complement(20492..20968)
                     /gene="gloA"
                     /locus_tag="WGR04_000522"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ0794139.1"
                     /translation="MADVYAKQNRSLTTTERNTKMRLLHTMLRVGNLEKSLDFYQNVL
                     GMKLLRRKDYPEGRFTLAFVGYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDA
                     YEACERVKRQGGNVVREAGPMKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(20932..22101)
                     /gene="lapB"
                     /locus_tag="WGR04_000523"
     CDS             complement(20932..22101)
                     /gene="lapB"
                     /locus_tag="WGR04_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ0794140.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINMHQTLLD
                     SPDTTGAKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDEQTYQFEIAQFYCELAQAALFKSNFDAARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKGEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDMNP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(22112..22426)
                     /locus_tag="WGR04_000524"
     CDS             complement(22112..22426)
                     /locus_tag="WGR04_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ0794141.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            22736..23734
                     /gene="ilvE"
                     /locus_tag="WGR04_000525"
     CDS             22736..23734
                     /gene="ilvE"
                     /locus_tag="WGR04_000525"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ0794142.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLTLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(23978..24763)
                     /gene="fabI"
                     /locus_tag="WGR04_000526"
     CDS             complement(23978..24763)
                     /gene="fabI"
                     /locus_tag="WGR04_000526"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ0794143.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGADLAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(24918..25739)
                     /gene="dapD"
                     /locus_tag="WGR04_000527"
     CDS             complement(24918..25739)
                     /gene="dapD"
                     /locus_tag="WGR04_000527"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ0794144.1"
                     /translation="MSLQNIIETAFENRADINPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEILNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(25906..27549)
                     /gene="pgi"
                     /locus_tag="WGR04_000528"
     CDS             complement(25906..27549)
                     /gene="pgi"
                     /locus_tag="WGR04_000528"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0794145.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYVDGRDVLPEIRRELNRALEFAHSLDDGSYQGITGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAEAV
                     KAWYRGAGFSESETGCHFCAVSADTEAAQSFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPVTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            27906..29138
                     /gene="pilG"
                     /locus_tag="WGR04_000529"
     CDS             27906..29138
                     /gene="pilG"
                     /locus_tag="WGR04_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ0794146.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            29212..30072
                     /locus_tag="WGR04_000530"
     CDS             29212..30072
                     /locus_tag="WGR04_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ0794147.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            30074..30706
                     /locus_tag="WGR04_000531"
     CDS             30074..30706
                     /locus_tag="WGR04_000531"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ0794148.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEIFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFISLIRRVLTISAPVEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHND"
     gene            30699..30908
                     /locus_tag="WGR04_000532"
     CDS             30699..30908
                     /locus_tag="WGR04_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ0794149.1"
                     /translation="MTESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(30993..32669)
                     /gene="pilB"
                     /locus_tag="WGR04_000533"
     CDS             complement(30993..32669)
                     /gene="pilB"
                     /locus_tag="WGR04_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ0794150.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
     gene            complement(32721..32822)
                     /locus_tag="WGR04_000534"
                     /pseudo
     CDS             complement(32721..32822)
                     /locus_tag="WGR04_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(33019..33090)
                     /locus_tag="WGR04_000535"
     tRNA            complement(33019..33090)
                     /locus_tag="WGR04_000535"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33055..33057),aa:Arg,seq:cct)
     gene            complement(33110..33557)
                     /locus_tag="WGR04_000536"
                     /pseudo
     CDS             complement(33110..33557)
                     /locus_tag="WGR04_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
     gene            complement(33562..34536)
                     /gene="ispB"
                     /locus_tag="WGR04_000537"
     CDS             complement(33562..34536)
                     /gene="ispB"
                     /locus_tag="WGR04_000537"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ0794151.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYALAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHEQALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(34552..34740)
                     /locus_tag="WGR04_000538"
     CDS             complement(34552..34740)
                     /locus_tag="WGR04_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794152.1"
                     /translation="MVNIRTPLCEIRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            34760..35068
                     /gene="rplU"
                     /locus_tag="WGR04_000539"
     CDS             34760..35068
                     /gene="rplU"
                     /locus_tag="WGR04_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ0794153.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            35093..35365
                     /gene="rpmA"
                     /locus_tag="WGR04_000540"
     CDS             35093..35365
                     /gene="rpmA"
                     /locus_tag="WGR04_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ0794154.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
BASE COUNT         8486 a         9870 c         8923 g         8125 t
ORIGIN      
        1 tctttaagtt gacatattgt gcgatatcga gggctgtacc gtattttccc atcgtatttt
       61 cccatcgtat tttcccatcg tattttcccg ttctttatat gagcggcagg catcctcata
      121 aaagaaggga taccgataag gaaaaatacc ccagcataaa aaatatactg attaatcaat
      181 acaaatccaa caaacagttc atcgtattct tttctgttgg cgtataaaaa tactgatata
      241 ttccttcact ttcatagggc gacgctttat gtaccgtcct gtgtgttgaa acgtcggttt
      301 tccttaccgc ctgccccgct tttccctgat tgctgccaac tgccgtccga acttgaaccg
      361 tcaggtttca gacggcattt tttctgcctg ctcccacaat tgccgttttt gtgcggcggt
      421 cagttttttg acggcgattt cctgcctgag tgccgtgcct ttatccatcc cgcctgcaac
      481 gatacgcatc gccaccggtt tgaatacgcg ggtatatttc gcgcctttac cggttgtgtg
      541 ggcggcaagc cgctgttgcg gattcgggct gatgccgcaa tagaacgcgc tgttttcaca
      601 caatatcagg taaacgctcc agtttgacgc gttcatctgc ctgcatcctg agttttctcc
      661 ccgttccaag gcgcaacttt cagcagcagc agcatacccg gtagggcgag tgcgaaacac
      721 agttggaaaa acggtacata accgagccat tcaatcagat aaccggcaaa ggaattgatg
      781 accgtgcgcg ggacggcgga caggctggta aacagcgcaa gctgcgttgc ggtaaatgcg
      841 ggattggtct cgcgcgccat atacgatacg aacgccgccg tccccaaacc cacgccgact
      901 gcctctgcgc cgataactgc cgccagcatc agcctctcgc ctgtgccgac cgtgtcgaaa
      961 tgtccgaacc ctgccagcca tacaaacccc aaaacggtta cagcctgcac cgcgccaaat
     1021 agccacaagg ctttgtttac gccgattttc agcatccaca cgccgcccaa gatacctgcc
     1081 gccactgccg gccacagtcc tgcatttttc gcaatcaaac cgatgtctgt cttgctgaaa
     1141 cccatatcca gataaaacgg cgttgccaac gcggttgcca tactgtcgcc gagtttgtaa
     1201 aggaagataa acagcagcac gcacaccgcc gaagcgatgc ccttgcgcgt aaaaaattct
     1261 ttaaacggct ctaccacggt ctgcttcaac gttttaggaa cggcaggcgg caacacgggt
     1321 tcgcgcgcga gaaacagcgt catcagaagg ccgggcagca taaataatga agtgataaca
     1381 aatacttctg accacggcat cctgtctgcc aacaccaaac tcaatgaacc gggaatcagg
     1441 gcggcaatcc ggtaggcgtt cacatgaacc gagttgccca aacccaattc ttcgtctgac
     1501 aaaatctcgc gcctgaacgc atccaataca atatcctgac tggcggaaaa aaaagcgaca
     1561 agcaccgaca agccggcaat cagcggcaga tgattacggg ggtttaaaaa ggcataggcc
     1621 gccaaagccg ccagcaaccc tgcctgcgtc agcagcatcc acccgcgccg ccgtcccaaa
     1681 acgggcagcc tgaccgcgtc catcagcggc gaccacaaaa atttccaagt aaacggcaga
     1741 ccgattaacg ccatcagccc gatgctcttc aaatccacct gctcgctgcg caaccacgcc
     1801 ggaatcaggt taatcagaaa gtacagcggc agccccgagg taaaccccgt aaaaatacag
     1861 acaagcatat ttcgggaaaa gatttgcccg ataaaacctg atttcgatgc agtcatcgtt
     1921 cgtgcagccc aaaaaaaaca aaagcctatt gtagcaaata tcgcccgcct gctgaaaaat
     1981 gccgccccgc gccgccggtt tgccgttcaa atgcaaaatg ccgtctgaaa ctttgttcag
     2041 acgacatttc tactgaaacc tgagagtttt acaggctgta atacatttca aattccagcg
     2101 gatgcggtgc catgcggata cggcggacat cttcttcttt gaaagcgatg tagctgtcga
     2161 tccagtcttt gctgaatacg ccgccgcgca ggaggaattc gtggtcggct ttgagggcgg
     2221 cgagggcttc ttccaaagag gcgcagacgg tcggcaccaa tgcatcttct tccggcggca
     2281 gatcgtacag gtttttatcg gcagggtcgc ccggatgaat tttgttttga ataccgtcca
     2341 aaccggccat caacaaagcg gcgaacgcca agtatgggtt ggcggtcgga tcggggaaac
     2401 gcgcttcgat gcggcgtgct ttgctgctgt tcacggacgg aatgcggatg gaagcggaac
     2461 ggtttttggc ggaataagcc agtttggtcg gcgcttcaaa gtgcggcaca aggcgtttgt
     2521 aggagttggt ggacggattg gtaatcgcgt tcagggcttt ggcgtgtttg atgatgccgc
     2581 cgatgtaata aagggcggtg tcgctcaaac cggcatagcc gtcgccggcg aacaggtttt
     2641 gaccgtcttt ccagatggat tggtgaacgt gcataccgct gccgttgtcg cccataatcg
     2701 gtttgggcat gaaggtggcg gttttgccga agttgtgggc aacgttttga atcacatatt
     2761 tcatgtcttg ggtttggtcg gcgcgtttga ccaaggtggc gaagcgcgtg ccgatttcca
     2821 tttggctgcc ggtgccgact tcgctgtggt gcacttcaac ttcgatgccg agtccttcca
     2881 aaatattcac catggcggag cgcaaatctt ggccgcaatc aatcggcgca acgggcgcat
     2941 aaccgccttt gacggtcggg cggtggccgg tattttgacc gtccatatgc aggccgcttg
     3001 cccaggcgcc gctttcggac gtgatttcgt aacgggtttt gtgcatatcg gtttcaaact
     3061 ctacgccgtc gaagacgaag aattcgggtt cggggccgaa gtatgccgtg tcgccgatgc
     3121 cggaagattt caaataggcc tcggcgcggc gtgcgatgga gcgcgggtcg cggtcgtaac
     3181 cctgaccgtc ggcagggtcg atgacgtcgc aggtaatgac gacggtaaca tcatcataaa
     3241 aaggatcgac gaaggctgta gacgcatcgg gacgcagctg catatcggaa gcctgaatgc
     3301 ctttccagcc gccgatggac gaaccgtcaa acgcctgacc gttttcaaac cactcttcgg
     3361 ggtcttccaa cacgatgcgc gcaggcacgg taaagtggtg ctgcttgcct ttggtatcgg
     3421 taaagcgcaa atcgacaaag cgggcttcgc tttcttcaat caattttacg gcgtttttaa
     3481 tagacatttt tcagctcctg gaaaagggaa atggcaaaat atgcggaacg cggcagatta
     3541 gctcaaagcc agcatgattc taccacgtcc atgccgtctg aaactggccc tgcgtcaaat
     3601 tccaagctgg aaaatatagt ttcagacggc aagattgtca acaatatgct tcggtttgtc
     3661 tgttcaaaag cagaataccc gcccttttat ccgaatgccg acaaaacaga tgccgtctga
     3721 aagcagttca gacggcatct gttgtttggt ttgaaaacgg tcagcgagga tagccgttgc
     3781 agacaaattt gtactgagcc tctccaagcg taccgggaat aacataattc cattcggcat
     3841 aggatggtgt ataagaagcg atagagtttc ccgatttgga ataccacgtt atcgtcaaaa
     3901 tcctccgacg tttaccactg caatccattt cttgcaatgc tctcactgta ttaaaaaatt
     3961 ttccattgcc aatcttttgc ggcttggcga accttgtttc aaaccatgca ctgttgtccg
     4021 tacggctgtc tgcatcacca aacgtggcag tattactatc taccaatacc caatccgccg
     4081 ccaccgccgg cgttgccaac cccaatgcaa atactgccaa gcacaacatc tttttcatgt
     4141 ccgaccctct cgtccttgtc aacaaaataa agttaaattg taaaaaaaaa acgacttttt
     4201 tcaagagaaa aatattccgt catgctatct gacaaccgtt tcagacggca tcgtttccat
     4261 ctgtacgccg ccgttataaa atagccttcc cgtcttttgg aacacgacca tgaccgccct
     4321 cccccgttac gccgttttcg gcaaccccgt tgcacacagt aaatctccgc agattcatcg
     4381 gcaatttgcc cttcaggaag gcgttgacat cgaatacgga cgcatttgcg ccgacatcgg
     4441 cggtttcgct caggcggttt cgacattttt tgaaacaggc ggttgcgggg caaacgttac
     4501 cgtaccgttc aaacaagaag cttttgcgct ggcggacgaa cattccgacc gcgctttggc
     4561 agcaggtgcg gtcaatacgc tgattctgtt gaaaaacggc aagctgcgtg gcgacaacac
     4621 cgacggtatc ggtttgacca acgacatcac gcaggtcaaa aatattgcca tcgagggcaa
     4681 aaccattttg cttttgggcg caggcggcgc ggtgcgcggc gtaattccgg tgttgaaaga
     4741 acaccgccct gcccgtatcg tcattgccaa ccgtacctac gccaaagccg aggaattggc
     4801 gcggcttttc ggcattgaag ccgtcccgat ggcggattta aacggcggtt tcgatatcat
     4861 catcaacggc acgtccggcg gcttgagcgg tcagcttcct gccgtcagtc ctgaaatttt
     4921 ccgcggctgc cgccttgcct acgatatggt ttacggcgac gcggcgcagg cgtttttgaa
     4981 ctttgcccaa agcaacggcg cggccgaagt ttcagacggc ctgggcatgc tggtcggtca
     5041 ggctgccgct tcctactccc tctggcgcgg atttacgccc gatatccgcc ccgttatcga
     5101 atacatgaaa gccctgtaaa catgttccgc atcatcaaat ggctgattgc cctgcccgtc
     5161 ggcatcttta tctttttcaa tgcctatgtg tacggcaaca tcattaccta ccgcgccgtc
     5221 gcgccccatc ggactgcctt tatgtcgatg cggatgaagc agtttgaaca ggaaggtcgc
     5281 gatgtcgcac tggattaccg ctggatgccc tacaaacgca tttccaccaa cctgaaaaaa
     5341 gccctgattg cttccgaaga tgcccgtttc gccggacacg gcggcttcga ttggggtggc
     5401 attcaaaacg ccatcaggcg caaccagaac agcggcgaag tgaaggcggg cggatcgacc
     5461 atcagccagc agcttgccaa aaacctcttc ctcaacgaaa gccgcagcta tctgcgcaaa
     5521 ggggaagagg cggccattac ggcgatgatg gaagccgtta ccgacaaaga caggattttc
     5581 gaactgtatc tgaattcaat cgagtggcac tacggggtgt ttggcgcgga agccgcgtcc
     5641 cgatactttt ataaaaaacc ggccgcagac ctgaccaaac agcaggcggc gaaactgact
     5701 gcccgcgtcc ccgccccgct ctactacgcc gaccatccga aaagcaaacg gctccgcaac
     5761 aaaaccaata tcgtgctcag acgcatgggt tcggcagagt tgcctgaaag cgacacggac
     5821 tgattgtttc agatatggaa atgccgtctg aactgcggtt cggacggcat atgttttctg
     5881 gtttttatag tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacaaat
     5941 agcacggaat cgattcactt ggtgcttcag caccttagag aatcgttctc tttgagctaa
     6001 ggcgagccaa cgctgtaccg gtttaaattt aatccactat acctgcccga cagccgcaaa
     6061 gaccgtatca tacatagcag gcattgcggt gcgggattac tgccgcaaac ggcaggcaaa
     6121 aaaaataacc gcttcaaggc ggttttttgg tggccagagg cggaatcgaa ccgccgacac
     6181 acggattttc aatccgttgc tctaccaact gagctatctg gcctttgtct gtctcgttaa
     6241 gagatgcgta ttaaaccaaa ttacgggacg acatgcaagc atatcgggca atattctttg
     6301 aaaacaggtt taagtcaatg ttttaaattt gaattaattt atgtaaaaac aacataaatc
     6361 cgtatttttg ccgcctgccc gacgatatta ggtcgcccgt ctgaaaaata ttttcaatat
     6421 ttgaagttct tacccagata aacagaacga acctgttcgt ttccgaccaa atcatcaggt
     6481 tttcccgatg ccagcaccgc accgtctgaa atgatgtagg cgcggtcgca gatgctgagg
     6541 gtttcgcgta cgttgtggtc ggtaatcagt acgccgatgc cgcgcgattt gaggaaaccg
     6601 atgattttct ggatatcgat gacggcgatg ggatcgacac ctgcaaaggg ttcgtccaaa
     6661 aggatgaagc gtggtttcat ggcgaggaca cgggcgattt cgacgcgtcg gcgttcgccg
     6721 cccgacaaag atggggcagg attattgcgc aaacgctcga tattcaggtc ggcaagcagt
     6781 ttttcaactt cgccgtcgat gcggcttttg tctttggtac tgatttctaa aatggcgcgg
     6841 atgttttgtt cgacggtcat tttacggaaa atagaggctt cctgcggcag gtagccgaca
     6901 cccaggcggg cgcgttcgtg tatgggcagg tgacgcaatt cctgcccgtc cagcatcacg
     6961 ctgcccgcat cggcggcaat gagtccaaca atcatgtaga agctggtggt tttacccgca
     7021 ccgttgggcc cgagcaggcc gacgacttcc ccgctttcga tttcaaggga gaagctttta
     7081 acgacttggc gttttttaaa actcttttgc aggttttgaa caacaaggcg gctgacgttt
     7141 gcgctcataa atatctcttc aatatcttcg gtcggcatat gccgtcggaa ccgggtttat
     7201 gtttcagacg gcattttggg attattcgga tttttgcgta ctcgaaggct ggatgatgac
     7261 gctgaccctg ccggatttgg aagcggattt tgcgccggat tttgtgctgc cgctgatggt
     7321 atagacttcg gttttggtgt tgtatgtaat caccgcgcct tcggcgacat cgccgccgcg
     7381 ctgtactttg gcattaccgg ttaagactac ggtgctgcct gcagacgaat aagcaacgtt
     7441 gtttgcctga ccgcgcaccg tccctttgcc cccgtccaac gtttggctga agcgtacggg
     7501 cgagccgtcg gccttcataa actgttcgcc tttgtcatta cgcgttacgt tgacgcgggc
     7561 ggcggaaata ttgagcgtac cctgtctgat gacaacgttt ccgctaaatg tagtgctttg
     7621 gttggcttga ttgagcgaac cttggtcggc ctcaatctga ataggctgcc tgctgtcgct
     7681 ttgaagggca aaagcgggag atgccgaaaa aaatgctatt aaaacaaata gcttacatat
     7741 cttttgtatc ataaatcgtg gctttcactt tagatgagaa gttcaacagg cctgttttgt
     7801 ggtcgtaagt catgccgccc gcctgcccgt gcgacgcgcc gtattgaaag ctgacaggcg
     7861 tatcggtttg ggcatattga gattcggtat cgacgtgcag cttttcggtt ttaactttac
     7921 ccgcctgccg tttgccatcg gcggttttgg tcagcacaac gttgttttta aaaagaacct
     7981 gtttgttttc ggtatggtaa acggcttcat cgctgccgac ttcgtacaac aacctgcctt
     8041 cttggaagaa cacgagatgc ggcgaatcaa aatggatgtc gctgctttcg ggaaactgtt
     8101 tcgcgccttt cgcgctcaaa tgttctttta agtatccctg ttcgtcaaac cgcctgccat
     8161 ccaatccgtc cattgtgtat tgcggttcgt cgggatttag cctgacttct tcaatctcga
     8221 cttcgctgat gcgtcccaac catgccgaca ggctgcccaa ggcaaccgcc aatatcaatg
     8281 ggaacgcaat tccgtaccgc catcttactt tcatttgatg tactcgttca aagccgcgcc
     8341 caaagtccct tgcgcctgca taatcaggtc gcacacttcg cgcaccgcgc ccgcaccgcc
     8401 ggggcgtttg gcaatatagg cggcgtgttg cagcgtaaac caatgcgcgt cggggacggc
     8461 aaccggcaat ccgcagcgta ctataacggg caaatcgacc acgtcgtcgc cgacaaaggc
     8521 acactcggct tcttccacgc cgacctgagc gcgcagctct tcatacgcgg cgcgtttgtc
     8581 cgaaatgcct ttgaagtagt aatttatgcc caactgtttg acgcggatgc cgacggaggg
     8641 cgcgtcccga cctgtgataa tcgcagtctg cacgccgctt gcctgaagca ttttcagacc
     8701 gtgtccgtcc agtgtgtgaa acgatttgat ttcttcgccg ttatcgcgga taaagatgcg
     8761 cccgtcggtc aaaacgccgt ccacatccag gatcagcagt ttgattttgg cggcgcgcgc
     8821 ctgtaattcg ggagaaattg cctgcatggg atttccttgt tttcagacgg catatcttac
     8881 cacaaatgcc gtctgaaacg gctgcaacgc cttatacaat ccgtgccgcc agcaggtcgt
     8941 gcatattcag cgcacctgtc agcacgccgt ctgcatcggt aaccagaagc ccgttcacat
     9001 ggtttgcctg catgactttc agggcttcgg tggcgaggcg ttcggcgggg atggttttag
     9061 gatgcgtatg catgacttct tctatcgaaa gaccggtaaa attgtcgcat tcttgaaaca
     9121 ggcggcgcaa atcgccgtcg gtgaatacgc ctttcagacg gcattgcccg tccgttaccg
     9181 ccagcatccc cagccctttt tcactcatgc tgacgatggc ttctttcaag ggcgtgccga
     9241 gtcggacggc aggcaggccg ccgcctttgt gcataatatc ggcaacgcgt aaaagcaggc
     9301 gtttgccgag gctgccggca gggtggctca aggcgaaatc gtcgggcgtg aacgcgcgcg
     9361 cgcgcagcag gacgaccgcc aacgcatcgc ccaaagccat aacggcggtg gtgctggtgg
     9421 tcggggcaag ccccagcgga caggcttctt tggaaaccga cgccgtgatg tggatgtcgg
     9481 catggcgcgc catggttgaa tcggggcggg cggtgatgca gacaagcgtg atgtttttgc
     9541 gtttgagcgc ggggatgacg gcggtgattt cgtcgctttc gccggaattg gaaatggcga
     9601 cgaccacgtc gttgtccaca atcatgccca aatcgccgtg tgccgcttcc gcagggtgga
     9661 cgaaaaacgc gggcgtgccg gtcgaggcca tggttgccgc cattttgcgt ccgatatgtc
     9721 ccgacttacc catacccatg atgacgaccc tgcctttgca gtgcaacaac gcgtccgccg
     9781 caagggcgaa gttttcgtcc aaatccgccg caatttcgcg caagccttcc gcttcggtgt
     9841 gaaacacttc gcgcgcccag tcgagatatt ttccgttttc tgccattgcc atccgccttt
     9901 gctgtaacaa aactacccaa aatttccgaa tcggggtagc ataagcattc aaaccccgcc
     9961 atccatccaa aggaatcaga aatgactatt ttatcggacg ttaaagcatt aggacaacaa
    10021 atctggctgg acaacctttc ccgctcgctg gtgcaaagcg gcgaattggc gcaaatgttg
    10081 aaacaaggcg tgtgcggcgt aacttccaat cccgccattt tccaaaaagc cttcgccggc
    10141 gacgcgcttt atgccgacga ggttgccgcc ctcaagcagc aagacctcag ccccaaacaa
    10201 cgctacgaaa caatggcggt tgccgatgtg cgtgccgcct gcggcatctg tcttgccgaa
    10261 cacgaatcca ccggcggcaa aaccggtttc gtcagcctcg aagtttcgcc cgaattgtcc
    10321 aaagacgcgc aaggcacggt agaagaagcg cgccgcctct acgccgccat cgggtgcaaa
    10381 aacgcgatga ttaaagtgcc tgccaccgac gcaggcatcg atgcgctcga aaccctcgtt
    10441 tcagacggca tcagcgtgaa cctgaccctg ctgttctcac gcgtccaaac cctcaaagcc
    10501 tacgccgcct acgcgcgcgg cattgccaaa cgcttggcag ccggacaaag cgttgcccat
    10561 atccacgtcg tcgccagctt cttcatctcc cgtgtggacg gcgcgctgga cacaacgctg
    10621 cccgaccacc tcaaaggcaa aatcgccatc gcccttgcca aagccgccta tcaagattgg
    10681 gaacaatatt tcaccacccc cgaatttgcc gcactggaag cccaaggcgc aaaccgcgtg
    10741 cagcttttat gggcatctac cggcgtgaaa aacccggcct atcccgacac gctctacgtt
    10801 gacagcctga tcggcgcgca caccgtcaac accgttcccg atgccacgct caaagccttt
    10861 atcgaccacg gcacagccaa agccacgctg accgaaggcg tagaagaagc acaagcgcag
    10921 ctcgccgaaa ccgccgcgct cggcatcgat gtcgaaacct tggcaacgcg tttgcaggaa
    10981 gacggtttga aacagtttga agaagcgttt gaaaaactgc tcgcgccttt ggcttaggca
    11041 ttttcccgat gccgtctgaa aggctttcag acggcatcgc ccgtttgacc ggacacagtg
    11101 cggcatatca aggattcacc ctaaaaatat taatgggcaa gaagttaatg cacgacatta
    11161 cccactaaca caaaaggaaa tagaatcatg gcaaaagcaa tcgaaatcat ttcacccaat
    11221 gaaatttatt cagatctgat ttttaaggat ccggtacctc cccatactat tgaatataaa
    11281 atgattaacc tagaattgaa agccatcaat ctttacgatt gtgcctttga agatttcgtt
    11341 cccgaaatca aagacaattt ttgtgtggga attgatttag acataggaac tgatgatagt
    11401 gatgctgcag atatattttc tgttcgcata tgctctccta aatggatttt gcataattgt
    11461 ttccaaaatc aaagggttaa atggggagcg ggtatgatga ttatgaatga atttaatcat
    11521 tctgttatta aatcggaaat tgagaaaatt cttaaagaat gttcaaaaga aacttgggaa
    11581 aagtcattga cttatttact tcgttttttt tcgtgggaat ttgaagatta tcagtgctaa
    11641 ttaaatcaaa aacagcgaaa ccaaaacttt ttcgctgttt tatttttcca taaacttttc
    11701 aaaatataca gaattgacga aactggttag tagaaatgca agtaatgaga gaattgcaga
    11761 tagagccttt gattttttct cgcctgctct tatggatggt tcagctaccg aagagcaata
    11821 taatgcccta tatgatttaa ccttgcttga agagcccggg atggaactga ataaagatga
    11881 gataatggct ttgattaata gtcttaaata aaggggctgt accagatatg tacacaggac
    11941 gttttgcccc cagcccgacc gggctgctcc acatcggctc gctgctgacc gccgtcgctt
    12001 cctatgccga tgcgcgcgcg cacggcggca aatggctgat ccgcatggaa gacctcgatc
    12061 cgccgcgcga aatgccgggg gcggcaagcc atatcctgca cacgcttgag gcattcggat
    12121 tcgagtggga cggagaagtc gcctatcaga gccgccgtta cgccctgtat gaagaaaccc
    12181 tatgccgcct gaaaaccgcc ggactggtct atccctgcca ttgcagccgc aaagactggc
    12241 aggccggggc aaggcggggc gcagacgggt tcgtctataa cggacgttgc cgccaccccg
    12301 gccaacgccc tgcactgcaa ggcaaacagc cggcgtggcg catccgcgtc cccgaccgca
    12361 ttatcggttt ttcagacggc atcgtcggcg gttacgccca aaacctcgcc cgcgacatcg
    12421 gcgatttcgt cctgcttcgt gcagacggtt actgggcata ccagctcgcc gtcgttgccg
    12481 acgatgccga acagggcgtt acccacatcg tccgcggaca agacctgctc gtttccacgc
    12541 cgcgccaaat ctatttgcag cagtgtttgg acgttccgac accgcaatat gcccacctgc
    12601 cgctgctgac caacgcacaa gggcagaaat ggtcgaaaca gacgctcgcc cccgcattgg
    12661 atttaaaccg ccgcgaacaa ctcctccgcc aagtgttccg ttacctcaag ctgcccgaag
    12721 caccggaaac cgaccgccct gccgaactgc tcgactgggc ggtggcacac tgggatatgg
    12781 acaaagtgcc gaaacacgcc gttaccgccc cctaaacacc gcccgacaaa cgcacaaatg
    12841 ccgtctgaag ccttcagacg gcatttcaca catacagccc cgccctattc gcgcatattt
    12901 gcccgttcga cctctttcca cgcttcgaga atcaggctgc cgtcgttgtc tttcaagagc
    12961 gttgcgtcgg aaagcatacg ccgccaagtc cgcgcgcctt tcagaccgtg catcagccca
    13021 aggctgtggc ggacgatgtg acgcaagatt gtgccgcgtc cggcttggat ttgggcttgg
    13081 ctgtatgtgt agagacgctg caccaaatcg gcgtattcaa tcgggctgcg ggtatcgccg
    13141 taaaacagcc tgtcccattc gcgcatcacc atcgggttgt ggtacgcctc gcgcccgacc
    13201 atcacgccgt caacgtgttg caggtgtccg gcgattgctt cgttggtggt gatgccgccg
    13261 ttgatgatga tttccagccc gggaaactcc tgcttgaggc ggtaaacgta atcgtatttc
    13321 aacgggggaa cgtcgcggtt ttctttgggg gaaagaccgt ccagccaagc gttgcgggcg
    13381 tggacgataa aggttttgca ggcggttttg tcgcgcagcg tgccgacgaa atcggcaacg
    13441 gtttggtatt cggtctgcct gtccacaccg atgcggtgtt tgacggtaac gggaatcttg
    13501 accgcatcct gcatggcgtt gaggcagtcg gcaaccagcc cgacttcgtt catcagacac
    13561 gcgccgaacg agcctttctg cacgcgcgga ctggggcagc cgcagttgag gttgacctcg
    13621 ttgtaaccgt atgcctcggc ggctttggcg gctttcgcca aatcggacgg atcgctgccg
    13681 cccagttgca gggcgacggg ctgctcgcct tcgttgaaca tcaaaaagcg gtctttgtcg
    13741 ccgtaaacaa tcgcaccggc attgaccatt tcgctgtaca gccaagtatt tcgggtaatc
    13801 tggcgggcaa ggtaacggta gtgcctgtcc gtccagtcga gcatcggggc gacggaaagg
    13861 cggcgcgggg gaagggtttg cgtgttgtcg ttcattgtcg atatttcacc attgcggatt
    13921 caatttcaat ccggaacaac cttcattttt ggggaaatcc gcgtcatgaa agagtccagc
    13981 agtatggtta ccggcccgtc gttacagagc gacacctgca tatgcgtgcg gaaacgccct
    14041 gtttcgacat gaatcccgtg tccgcgcaac agttccgccg ttcgcaggta aagctgctgc
    14101 gcctgttctg caggtgcggc ttgggaaaac gaaggccgcc gcccgcttgc cgcatcggca
    14161 taaagtgtaa actgcgacac cagcagcacc gcgccgccga catctttcaa agacaggttc
    14221 aacttgcccg cttcgtcttc aaacacgcgc aaatgggcga ttttgtcggc gatatagcgt
    14281 gcatcttttt ctgtgtcgct atgcgttaca ccgagtagca cgacgaatcc gccgtcgatt
    14341 ttgccgcagg tttccgtgcc gttttcggac acgacatcca cctttgcacc taccgttttc
    14401 tgtatgaccg cacgcataag ccgtcctcct tacctgaacg tgcaatgccg tctgaaaagg
    14461 cttcagacgg catcggtatt ggaaatgcca ttacggtttg acaccgtttt cttccaaaat
    14521 ggcatcgatt ttcaagcggg ctttttcctg tctcaaacct tgttccaact ggtttctgac
    14581 caactcgaaa ggctgcgcgt cggggttttt cccgacctcg ctgagtttga acagataata
    14641 gcgttcgccc aatttgaccg gatcgcgggt aacgtcgccc cgattcatcg ctgcaaactg
    14701 cgaagccagc ggctcgggaa gctgctgcgc cataatgaaa ccgtcaaacg cctgctcgtc
    14761 gttcggataa cgcttcatca gcccttcaaa agacagccct ttgagcagga gctgctgctc
    14821 ctgacgcgcc tcctcttcgg ttgcgaagct gacctgctgc aatttgatca tgcggatttg
    14881 ccgctcataa aactgacgca gtgcgctttc ggaaaccgtt tccgaacgtt ccagaaaacg
    14941 gacgtactcc tcggcataaa aagacgcttc ggcgatttta aagcggtttt ggacatcctt
    15001 atccttatcc aaaccttcct tcaatgccct gtttttcaaa acttccaaag tttgcagccg
    15061 acggacggca tcgtttcgga ttgcctgccc gtccggtttt tgggactgct ccgcatgccg
    15121 gtctgcctgc tgcatgatct gcgccaccag cgtatccacc aaagccgggt cgatttcggg
    15181 tgctttggct gccgcaaaac ctgccaacat tgcagcaata actgcggcag cggttttttt
    15241 ctgtttcatg gtattccctg tcctgcgcgg cggcaaaacg cctgtctgaa cgtcttgccg
    15301 ccatatcccg ttttcagaat tattttgcag gtttgatgtt tgccttgccc aacagtgcac
    15361 cgacggcacg gtcaatccgt tccgcctgaa ggttgcccgc aatctgtcct ttcatttcat
    15421 caaaagaagg cacttttacc tcgcggctgt cgttgacata ataaacgccg tagaaatcgc
    15481 cgtttttcag cggcgttgcc gtaaattcgc cttttttcaa gtccttaatt gcctgataaa
    15541 gcggcggaac accctgttcc aaatctttca gcggcacata tccgaccggc gcaccggtct
    15601 gtttggtacg gtcgttgagg gaatattgtt tcaagacggc atcgaaacct tttttcgcct
    15661 tcaagtcggc aaccgctttt tttgcatttt cttccttgtc ggtcaggatt tcgcccaact
    15721 ggacttcctg cgtacctttg taaaaaccgc tgatattgtc atatgcggct tttacttcct
    15781 gctcggaaac cggttgggtt ttggcgatat gcaatgcgta tgcctcgccg ttcaagccat
    15841 attttaccgc ctgccaaacg gttttgaagg acggtttctt gtcgtcgccc gactttttcg
    15901 cttcggcacg caatttggca agcgcatttt taaactctgc cgaccggtcg agtttcaggc
    15961 gtttcacttc ctgtgcgacc acggtattga ccacttcgtt ttccagcagg gattggcgca
    16021 gttgcggcgt gtcttcggca cggctgtttt ccgcacggaa tgcggcaacc tgcgcatcga
    16081 tgacggaact gtcgattttc tgaccgttga cggttgccag cgtttgggca aacaggctgc
    16141 cggaacaggc aagcagtgca acggaagtca ggatttttgc tttcataatc ttctctcttt
    16201 aaattttaaa ttgaaatgtt cgggcagact tcattcagtc cgccgtatgg aaatactcgt
    16261 caggggtagc cgcccggatg ccgagcgcgt gaatcatgcc gcctgaaaac aaatctttga
    16321 gcagcgattt gaccgttttc tggcggttca ggcggcttac gccttcaaaa cggccgctaa
    16381 cgaccaaaac ggcataatgt ccgccgccgg tattgcccgc gtgtcctttg tgcagatggc
    16441 tctcatcgcc gatttccaac accagcggat cgagcgtctg caggcgttcg cggatcaaat
    16501 cgacggccgg cattatttga acaccgcttt aaacggcttg atcagcactt cgctgtacac
    16561 gcctgcatga acatagggat cgtcttccgc ccaagcctgc gccgcatcca aagactcgaa
    16621 ctgcgccaca atcaaactgc ccgaaacgcg gttggagtcc tccggcaaag gattcgggcc
    16681 tgccgtcaac agccggcctt ccgacttcag cgtctccagc cgtttgaggt gttcgggacg
    16741 tgccgccata cgcgcctcgt gcacatcctc cccgtctgtt gccagcaaca taaaatattc
    16801 cacagtcaat cctccttttt cagacaggta ctcagataaa taccctgaat aataaagaaa
    16861 acaagcatca gtgcagtcga gccgaacatt ttatagttga cccattgcga ctcgaaccgg
    16921 gtaaacacaa accagttggc gatacccata aaaatcagga aaccgaccca catatacgtc
    16981 aatttcgccc atacggcatc cggaagctga atctccctgc cgatactcgc cttcaagccg
    17041 tttttaccgg cgaggtggct gccccacagg aacagcgcgc ccagccaaaa caaaacgctc
    17101 ggcttccaca taatgaagcg gctgtcgccc aaaacaatgg ttgcgccgcc gaataccaca
    17161 atcagcacca atccgaccca ctgcatcgta tccagctttt tatatttcca atacagaaaa
    17221 gccgcctgaa ccacaccggc aaccaaggca accgccgtag cggcaatcat atttttggta
    17281 acggtataag tagcaaaaaa caagatgaca gacaaaaggt cgctgacgaa tttcatatcc
    17341 aaatgagaaa gcaaatcaaa taaacgctaa tcatacaggc agaaccctgt ttttgcacct
    17401 gcaaaaccaa caaattttgt tttgattgat gcaaatatgc acagatgttt ttgaaaaaag
    17461 atggagatat gtcataattt gtaaaaacgg cgaatgattg ccgtttaaaa tgtggcggcg
    17521 gtcggtacat tcacatatta aatgcccgcc ggccgactgc cccgccgatg cccgatgatg
    17581 acgaaattta cagactgtac gcggtcaaac cgtattcagc cgccaaccca caggggatac
    17641 atcttgaaaa acaacagaca aatcaaactg attgccgcct ccgtcgcagt tgccgcatcc
    17701 tttcaggcac atgctggact gggcggactg aatatccagt ccaaccttga cgaacccttt
    17761 tccggcagca ttaccgtaac cggcgaagaa gccaaagccc tgctaggcgg cggcagcgtt
    17821 accgtttccg aaaaaggcct gaccgccaaa gtccacaagt tgggcgacaa agccgtcatt
    17881 gccgtttctt ccgaacaggc agtccgcgat cccgtcttgg tgttccgcat cggcgcaggc
    17941 gcacaggtac gcgaatacac cgccatcctc gatcctgtcg gctactcgcc caaaaccaaa
    18001 tctgcacttt cagacggcaa gacacaccgc aaaaccgctc cgacagcaga gtcccaagaa
    18061 aatcaaaacg ccaaagccct ccgcaaaacc gataaaaaag acagcgcgaa cgcagccgtc
    18121 aaaccggcat acaacggcaa aacccatacc gtccgcaaag gcgaaacggt caaacagatt
    18181 gccgccgcca tccgcccgaa acacctgacg ctcgaacagg ttgccgatgc gctgctgaag
    18241 gcaaacccaa atgtttccgc acacggcaga ctgcgtgcgg gcagcgtgct tcacattccg
    18301 aatctgaaca ggatcaaagc ggaacaaccc aaaccgcaaa cggcgaaacc caaagccgaa
    18361 accgcatcca tgccgtccga accgtccaaa caggcaacgg tagagaaacc ggttgaaaaa
    18421 cctgaagcaa aagttgccgc gcccgaagca aaagcggaaa aaccggccgt tcgacccgaa
    18481 cctgtacccg ctgcaaatac tgccgcatcg gaaaccgctg ccgaatccgc cccccaagaa
    18541 gccgccgctt ctgccatcga cacgccgacc gacgaaaccg gtaacgccgt ttccgaacct
    18601 gtcgaacagg tttctgccga agaagaaacc gaaagcggac tgtttgacgg tctgttcggc
    18661 ggttcgtaca ccttgctgct tgccggcgga ggcgcggcat taatcgccct gctgctgctt
    18721 ttgcgccttg cccaatccaa acgcgcgcgc cgtaccgaag aatccgtccc tgaggaagag
    18781 cctgaccttg acgacgcagc agacgacggc atagaaatca cctttgccga agtcgaaact
    18841 ccggcaacgc ccgaacccgc tccgaaaaac gatgtaaacg acacacttgc cttagatggg
    18901 gaatctgaag aagagttatc ggcaaaacaa acgttcgatg tcgaaaccga tacgccttcc
    18961 aaccgcatcg acttggattt cgacagcctg gcagccgcgc aaaacggcat tttatccggc
    19021 gcacttacgc aggatgaaga aacccaaaaa cgcgcggatg ccgattggaa cgccatcgaa
    19081 tccacagaca gcgtgtacga gcccgagacc ttcaacccgt acaaccctgt cgaaatcgtc
    19141 atcgacacgc ccgaaccgga atctgtcgcc caaactgccg aaaacaaacc ggaaaccgtc
    19201 gataccgatt tctccgacaa cctgccctca aacaaccata tcggcacaga agaaacagct
    19261 tccgcaaaac ctgcctcacc ctccggactg gcaggcttcc tgaaggcttc ctcgcccgaa
    19321 accatcttgg aaaaaacagt tgccgaagtc caaacaccgg aagagttgca cgatttcctg
    19381 aaagtgtacg aaaccgatgc cgtcgcggaa actgcgcctg aaacgcccga tttcaacgcc
    19441 gccgcagacg atttgtccgc attgcttcaa cctgccgaag caccgtccgt tgaggaaaat
    19501 ataacggaaa ccgttgccga aacacccgac ttcaacgcca ccgcagacga tttgtccgca
    19561 ttacttcaac cttctgaagt acctgccgtt gaggaaaatg cagcggaaac cgttgccgat
    19621 gatttgtccg cactgttgca acctgctgaa gcaccggccg ttgaggaaaa tgtaacgaaa
    19681 accgttgccg aaatacctga tttcaacgcc accgcagacg atttgtccgc attacttcaa
    19741 ccttctgaag tacctgccgt tgaggaaaat gtaacggaaa ctgttgccga aacgtccgac
    19801 ttccacaccg ccgcagacga tttgtccgcg ctgctgcaac cttttgaaac acctgccgtt
    19861 gagaaatttg aagcaccggc tattgaagaa aaagcagcgg aaaccgttga taaaacgccc
    19921 gacttccaca ccgttgccga cgatttgtcc gcgctgctgc aacctgctga agtacctgcc
    19981 gttgaggaaa atgcagcgga aatcactttg gaaacgcctg attccaacac ctctgaggca
    20041 gacgctttgc ccgacttcct gaaagacggc gaggaggaaa cggtagattg gagcatctac
    20101 ctctcggaag aaaatatccc aaataatgca gataccggtt tcccttcgga atctgtaggt
    20161 tctgacgcgc cttccgaagc gaaatacgac cttgccgaaa tgtatctcga aatcggcgac
    20221 cgcgatgccg ctgccgagac agtgcagaaa ttgctggaag aagcggaagg cgacgtactc
    20281 aaacgtgccc aagcattggc gcaggaattg ggtatttgat tcccaactgc cctttcgcag
    20341 atcaaggatg ccgtttcaga cggcatcttt ttttgcctta tcggtgtaac ggataaagtt
    20401 tgaaccggca caggctcaaa cagcaggtcg acggcaacaa aatgccgtct gaaaccccta
    20461 aaggcttcag acggcattgg cggcggcggt atcaggcagt ttgataggca accgaatcgt
    20521 cgccgctttt cttttgaatg aactcgattt tgtatccgtc ggggtcttcg acgaaggcta
    20581 tcacggttgt gccgtgtttc atcgggccgg cttcgcggac gacgtttccg ccctgccgct
    20641 tcacacgttc gcaggcttcg taggcatcgt ccacttcaac cgcgatgtgt ccgtaggcgt
    20701 tgcccaagtc gtatcgttcc gtatcccagt tgtgcgtcag ttccaaaacc gtgctgtcgg
    20761 tttcatcgcc gtaaccgacg aaggcaaggg taaatctgcc ttcgggataa tcttttcggc
    20821 ggagcagttt catacccaaa acgttttggt agaaatcgag ggatttttcg agattgccca
    20881 cgcggagcat agtatggagt aagcgcattt ttgtgttcct ctcggtagtg gttaaacttc
    20941 gattttgttt ggcgtaaacg tctgccattt gttgcaggca ggacaatgcc agaaaaagac
    21001 ttgtgatttg aagtggcagt ttcggcaccg gtacatcacg ctgcgctgta gctgccgtcc
    21061 gataaccgaa cgcatcatgt cggcatcggc tttccaagcc ggattcatat cgctgagttt
    21121 caaaccgagc aggcggtaca cgccgttaag gtcgggcttg cggcggacaa gctcgacggc
    21181 ggtttgcgcg gcttctttct cgcccttaag cagcagggat ttctcgtaca cgacattgat
    21241 caggtcaagt tcgggaaacg tctgcatata tcctgtcaga cggttcaagc cttcttcagg
    21301 ttttccctgc gcggcatagg cttcgtaaag cttctcgccg accatgctca agtatgcatg
    21361 gttttgctgc tcgatggcgg cataggcttc gacggcggca gggaaattgc cttgtcggtg
    21421 ttcgatgtcg cccaaaatca tgttggcgcg ggtgcatttt ttgttggctt cgagtgcctt
    21481 gccgacattg aaacgcgcgg catcgaaatt ggacttgaac agcgcggctt gggcaagttc
    21541 gcaatagaac tgtgcaatct caaactgata ggtctgctcg tcatggctga gcagccgggc
    21601 ggtttcaacc gctttttccc aatccctgtc ctgttggtag atattgagca ggtgctgtct
    21661 ggcttcacgc gccatttcac cgtcttgcag ccccaaaaaa atctgttcgg cacggtcaac
    21721 caaccccgca ctttgatagt tttgcgccaa ttcaaataat acgcgcgcac gcttggctcc
    21781 ggttgtatcg ggagagtcaa gcaatgtttg gtgcatattg atggctttgt cgttttcgcc
    21841 acgctgacgg taaagcttgc ctagggtgag gttcaggtcg tatgactgag gctgctgatc
    21901 gatgacctct gccaattccc tggcggcacg tccgctgttg cggtcaacca aggcatccag
    21961 acttttataa aatcccgacg gtatgctttt tgcctgcttt aatacagtct tcatatccac
    22021 gcgggcggca aaccagccca tcgcgaagaa aacgggcaaa aggataatcg gcagcaggat
    22081 aatccacaat tcattgtcca tatcggcttt ctcaaggctg tttggcagat tcgggcgcat
    22141 tttgcgccgg tggtgcggtc agctccttcc ccgtcaaacg cgcatttttc tttacttcgg
    22201 cacgcaacct gccgttctcg ccacgtaacg acaacaaccg tccgaacaag gcaaacattc
    22261 caaaaacaat accgactaca aatgcgccga acaatacgac aatcagcggc aaatcgaatt
    22321 tttgccccgg caggtaggaa aaggtaacgg catccgtatt aatgacagca agcagcagga
    22381 agagcagcag gataatgatt ttgatgacgg tatagataag tttcatgttt gctccgtaaa
    22441 taaaaacgcc gcccggacag cttcgcactc tgaaccaaga ttgcgctagt ttaaacgatt
    22501 tgcacggttt cggataggat gcggcagcgt gttcggacga catacggagt atgcgattgc
    22561 cgacaatttt accaaataca ccgttccttt ccatttgaaa aataacggat tggacaccgc
    22621 atcgacagaa aaacccgccg cgcacttgtc aaaaccctgt tggcaggcgt atctttacaa
    22681 tcttcaaatt caaaccgttc attgaaacat atcagaataa gaaaggcttt acatcatgag
    22741 cagacccgta cccgccgtat tcggcagcgt ttttcacagt caaatgcccg tcctcgccta
    22801 ccgcgaaggc aaatggcagc cgaccgaatg gcaatcttcg caagacctca ccctcgcacc
    22861 cggcgcgcac gccctgcact acggcagcga atgctttgaa ggcttgaaag ccttccgtca
    22921 ggcagacggc aaaatcgtgc tgttccgtcc gactgccaac atcgcgcgta tgcggcaaag
    22981 tgcggacatt ttgcacctgc cccgccccga aacccaagcc tatctcgatg ctttggtcga
    23041 actggtcaaa cgcgccgccg atgaaattcc cgatgcgcct gccgccctgt acctgcgtcc
    23101 gaccttaatc ggtaccgatc ccgttatcgg caaagccggt tctccttccg aaaccgccct
    23161 gctgtatatt ttggcttccc ccgtcggcga ctatttcaaa gtcggctcgc ccgttaaaat
    23221 cttggtggaa accgaacaca tccgctgcgc cccgcacatg ggccgcgtaa aatgcggcgg
    23281 caactacgct tccgccatgc actgggtgct gaaggcgaaa gccgaatatg gcgcaaatca
    23341 agtcctgttc tgcccgaacg gcgacgtgca ggaaaccggc gcgtccaact ttatcctgat
    23401 taacggcgat gaaatcatta ccaaaccgct gaccgacgaa tttttgcacg gcgtaacccg
    23461 cgattccgta ctgacggttg ccaaagattt gggctatacc gtcagcgaac gcaatttcac
    23521 ggttgacgaa ctcaaagctg cggtggaaaa cggtgcggag gcgattctga ccggtacggc
    23581 agccgtcatc tcgcccgtta cttccttcgt catcggcggc aaagaaatcg aagtgaaaag
    23641 ccaagaacgc ggctatgcca tccgtaaggc gattaccgac atccagtatg gtttggcgga
    23701 agacaaatac ggctggctgg ttgaagtgtg ctgatgcttt aaataaaaaa tgccgtctga
    23761 aacccgtttg gcgtttcaga cggcattttc gcatccgaac cgtttccgct gcacctgcag
    23821 caagtcggca caaaggcaat cggttaaaac aagcgtccgc atttcccatc ccgcctgccg
    23881 taagtcgggc atttccctag aaatacgctt cagacggcaa aacgccgccc gaaaccgata
    23941 tgcggcacgg acggcgcacg gatttgaaaa cggcggatta tccctcggtg ctcaaggcat
    24001 tgatgctgta accgccgtca acgtaagtga tttcgccggt aatgccggac gacaggtcgg
    24061 acagcaggaa ggcggcggta ttgccgactt cttcgatggt aacgttgcgg cgcagcggat
    24121 tgtgggcggc gacgtgtccc aagagtttgc cgaaatcggc gatgccggag gcggcgagtg
    24181 ttttgatcgg accggcggaa atgccgttgc agcggatgcc ttctttgccc agacaggcgg
    24241 cggtaaaacg gatgcccgcc tcaaggctgg cttttgccat acccatcacg ttgtaattcg
    24301 gaatcgcgcg caccgcgccc aagtagctca gggcaacgat ggcggagttt ctgccgcgca
    24361 tcatcggacg ggcggctttt gccaatgcgg gcaggctgta tgcggaaatt tcgtgtgcgg
    24421 tgttgaacgc ttcgcggctg atgctgtcga ggaaatcgcc gctcaaggct tctttcggcg
    24481 caaaaccgat ggaatgcacc aaaccgtcca agccgtccca atgtttgccc aaatcggcga
    24541 acacttggtt gatttcgtcg tcgctggcga catcgcagcg gaatacgagt tcggaatcca
    24601 attccgccgc cattttgcgg acgcgctctt ccagtttgtc cacaacgtag gtaaacgcca
    24661 aatccgcgcc ttgttcgcgg caggctttgg cgatgccgta agcgatggaa cgctcggaaa
    24721 tcatgccggt aatcagaatt tttttgcctt gcagaaagcc cattttgtat ccttcaaata
    24781 gtgttcggtt cgttctaaac cgttgattat agcaaattgt ccctgtttct gtgttttcac
    24841 gttgcagcgt gcaaacggca atgccgtctg aagcggattt cagacggcat cggacgtttc
    24901 aaatacggtt taaggcatca gatgccgcgc aacaattcgt tcacactggt tttggcgcgt
    24961 gtttgcgcgt ccacacgttt gacgatgacg gcgcagtaaa ggctgtggct gccgtctttg
    25021 gaaggcatgc tgccggatac gacaaccgaa cctgccggta cgcggccttg gtagatttcg
    25081 ccggtcgtac ggtcaaagat tttggtggat tgaccgatga atacgcccat cgaaatcacg
    25141 ctgccttctt cgacaatcac accctcaacg atttcggaac gcgcaccgat gaagcaattg
    25201 tcttcaataa tggtcgggct ggcttgcagg ggttcgagta cgccgccgat gccgacaccg
    25261 ccgctcaagt ggacgttttt gccgatttgc gcgcaagagc cgacggtcgc ccaagtatcg
    25321 accatcgcac cttcgtcaac gtatgcgccg atgttgacgt aagacggcat cagcacgaca
    25381 tttttcgcca caaagctgcc gcgacgcgca accgcacccg gaacggcgcg gaagcctgcg
    25441 tttttgaact cgtcttcaga ccagtcggca aacttggtcg gcactttgtc gaagtatttg
    25501 ttcacgccgt cgttgaggat ttcgttgtct tggatgcgga aggacagcaa cacggctttt
    25561 ttcgcccatt cgttaacttt ccattcgccc acgcccaagc gttcggcgac gcgcagtttg
    25621 ccggaatcga gttggcggat ggtttccaac acggcttctt taacttcggg agtaacggtg
    25681 gtcggattga tgtccgcgcg gttttcaaag gcggtttcga taatgttttg caaagacata
    25741 atgtttcctt atgtgagatg tttcgtggga cgcttgccgg tattttcaag cagtatgttc
    25801 atttgaaatg aatgcgctat tgtgccacgc aaacgcatat tcgtcagcaa tacgcgggcg
    25861 gttgccgaac attggcgttt cggaatcggg cgggcggcag tacctttatg ccgcaccgcc
    25921 gcctttcaga cggcattcgc ggtaaaacgc catcagccct tcggtcgagg catcgtgtac
    25981 ggacgtgccg ccttccagtt cgccgatgat ggttttcgcc aactgtttgc cgtattccac
    26041 cccccactga tcgaaggggt tgacgttcca tatcgcgcct tggacgaagg ttttgtgttc
    26101 gtaagccgcc atcagcatac ccaaattgta gggcgtgagg cggtcgagca ggatgctgtt
    26161 gctggggcgg ttgccaggga actctttgtg cggcgcaagg cgttcgcgtt ccgcttcggg
    26221 caaatctgcc agttcggcgc gtgcttcgtc caaggttttg cccttcatca gggcttccgc
    26281 ttgggcaaag gcgttggcaa cggtaaaacg gctgcgtccg tcctccacgc cctgcgccgt
    26341 caccgggacg ataaagtcgc agggaatcag gcgcgtgcct tgatgaagca gttggaaata
    26401 ggcgtgctgg cagttgaccc cttcaccgcc gaacacgatg ccgcccgttt tgcacacggc
    26461 gggactgccg tctgaagcgc ggcttttgcc caaactctcc atatcgagtt ggttcagcca
    26521 cgccggcagc aggcgcaggt tgtggctgta cggaacggcg gtctgcccgt ccgcgtgttg
    26581 gaaattgtta taccacacgg caatcagtgc catcagaacg ggaatgttgc ggcgcggcgg
    26641 cgtgtggaaa aaatggctgt ccatcgcgtg cgcccccgcc aacaactcgc ggaaacgcgc
    26701 cccgccgacc gcaaccatca cgggcaaacc gacgggcgac cagacggaat agcgtccgcc
    26761 cacccagtcg tacatcgcaa acacgcgttc cgccgcaatg ccgaaacttt gcgccgcttc
    26821 cgtatcggca gataccgcgc aaaaatgaca tcccgtttcc gattccgaga accccgcacc
    26881 acgataccaa gccttgaccg cctctgcatt gagcagggtt tcaggcgttt tgaaggactt
    26941 gctggcaacg caaaacactg tcgtttcggg gttcagacgg cataaaaccg catccaggca
    27001 ggcaggatcg gcgttggcgg caaaatggac ggcgatatgc cgtctgaacg gctcaagtgc
    27061 ctgcacgcac attgccggcc cgaggtcgga tccgcctatg ccgatgtgga caaaatccgt
    27121 aatccgtttc ccagttatcc cctgatacga accgtcgtcc aaactgtgtg caaactccaa
    27181 cgcacgattt aactcgcggc ggatttcggg caacacgtcc ctgccgtcca cataaacggc
    27241 atccgcaccg tcgggcaggc gcaaagccgt atgcagcgcg gcacgcccct cgctgccgtt
    27301 gactttcgca cccgtccgca aagcacgcat tttcccttcc aaatccgccg tctccgcaag
    27361 acggcagagc agttgcagcg tatcttcgcc caaacggttt ttgctgtaat cgaacaacat
    27421 cccgtccaaa cgctcatgca tacgctcaaa acggtcgggt tcgcaggcaa agcggtcgcg
    27481 caaaaggaca tgacgcgtat cctgataatg gcgttcgagc gcataccatg cacgggtaaa
    27541 agcattcatc tgttttcctt gatttttcag aaccggatta aaatgtagca gaatgtagtt
    27601 taacaaacgg cagcggcttt ggcgaatctc cagaacaccg cccgccgcgt ccaatagcac
    27661 cttatttcca tatggcggaa atgccctccg gccggcattt gacccgcaac aatatcctgc
    27721 aagatttatt gtgtacgcat aaatgccgga cagccgccta aataaggtat aatcgcatcc
    27781 gattctgtcc cgtttgccgc ccgcaggcgg gatgtcggtg gttttcatct taccgggaac
    27841 acgccggtac tgttttttaa cgatgtttct tacattttat tccaattact ttacggggct
    27901 agaatatggc taaaaacgga ggattttctt tgttcgcaaa gaaagaaaaa cgctttatct
    27961 ttgaaggcag gcattccgcc tccgacaaac tggtcaacgg cgaagtatcc gcgtttaccg
    28021 aagaagaggc gcgcaaaaaa ctggcaaaac gcggcatccg cccgttgcag attactcgtg
    28081 tgaaaacaag ctccaagcgc aaaatcacac aagaagacat caccgttttc acccgccagc
    28141 tgtccacgat gattaaagcg ggcctgccgc tgatgcaggc atttgaaatc gtggcgcgcg
    28201 gacacggcaa cccgtctatg acggaaatgc tgatggaaat ccgaggcgaa gtggaacagg
    28261 gcagctcgtt gagccgcgca ttctcaaacc acccgaaata tttcgaccgc ttctactgca
    28321 acctggttgc ggcaggcgaa acgggcggcg tattggaaag cctgctggac aaattggcaa
    28381 tttacaaaga aaaaacccag gctatccgca aaaaggtaaa aaccgcactg acctatccgg
    28441 tatccgtcat cgccgtcgcc atcggtttgg tattcgtgat gatgattttc gtactgcccg
    28501 cctttaaaga agtttacgcc aatatgggcg cggagcttcc cgcactgacc caaacagtga
    28561 tggatatgtc cgactttttc gtctcatacg gctggatggt gctgatcgca ctgggctttg
    28621 ccatatacgg cttccttaaa ttgaaggcgc gttcgattaa aatccaacgg cgtatggatg
    28681 ccatactgct gcgtatgccg attttcggag acattgtccg caaagggacg attgcccgct
    28741 ggggcaggac gacagcgacg ctgattgcgg caggcgtgcc tttggtcgat gtattggact
    28801 ccactgccgg cgcggcgggc aatttaatct atgaagaagc cacccgggaa atccgtacgc
    28861 gcgtcatcca aggtttgtct atgacttcgg ggatgcgtgc gacggaactg ttccccaata
    28921 tgatgttgca gatgtcttcc atcggcgagg aatcgggttc tttggacgat atgctcaaca
    28981 aagccgccga attttacgaa gacgaggtgg acaatgcggt cggcaggctg tccgctatga
    29041 tggagccgat cattatcgtg attttaggct tggtcatcgg cacgcttctg gtcgccatgt
    29101 atctgccgct gtttaatttg ggcaacgtgg tcgcctgatt tgccgcacag atccggcgcg
    29161 gattcgctct gcgccggttt gtttttgttt tgaatacatt gagaacaaaa tatgtctgat
    29221 ttgtctgtat tgtcgccgtt tgccgtgcct ttggcagcgg tgttcgggct gctggtcgga
    29281 agtttcttaa acgtcgtcat ttaccgcgtg ccggtcatga tggaacgcgg ctggacggta
    29341 tttgccaaag aatatttaaa cctgccgctg accgaagagg aaagccgtac cttcaacctg
    29401 atgaaaccgg attcctgctg tcccaaatgc cgcgtgccga tacgcgcgtg gcagaacatc
    29461 ccgattgtca gctacctgct cctgcgcggc aaatgcgctt cctgccaaac caaaatcagc
    29521 atacgttatc ccttaatcga gctgctgacc ggcgtattgt tcgggctggt tgcctggcaa
    29581 tacggctggt cttggattac attgggcggt ttgatactga ccgcgtttct gatttccctg
    29641 acctttatcg atgcggacac ccaatacctg cccgactcga tgacactgcc cttgatttgg
    29701 ctggggctga tatttaattt ggacggcggc ttcgtgcctt tgcagtctgc cgttttaggt
    29761 gcggttgccg gctatggttc gatatggctt ttatgcgcgg tatataaact gattaaaggc
    29821 gaaatcggta tgggcaacgg agatttcaaa ctgattgccg cattgggcgc gtggctcggc
    29881 atatccgcat tgcccgtgct gatttttgtt tcctcgctga tcggtttggt cgcggcaatc
    29941 gttatgcgcg tcgccaaggg gcagcatttt gccttcggcc ccgcactgac agtttcgggc
    30001 tggataattt ttacggcaaa cgattccgta tggcgggcgg tcaactggtg gctgacccat
    30061 ccggtgctgt aagatgacgg tatgggtcgg gctgaccggc ggaatcggca gcggcaaatc
    30121 ggcagccgcg caatgttttg ccgatttggg cgtaccgcgt atcgatgcgg atgcggcggc
    30181 gcactcgctg acggcttcag acggcatcgc cctgccggaa atcaggcggc tgttcggcga
    30241 caccgttttc gacacacagg gtttgttgcg gcgcgacata ttgcgtaaag aaatctttgc
    30301 ctccccatcg cgaaaagcct tgctcgaatc cgtgatgttg ccgctgattt tctcagaaat
    30361 caaaaaacag caagaaacct ttaccgatgc ggcttacggc attgtcgaaa ttccgctgct
    30421 gacggaaaag cgtcaattta tcagcctgat acggcgtgtc ctgaccataa gtgcccctgt
    30481 ggaaaaacgt atcggcaggg tgatggcccg cagcgggctg acgcgcggcg aggtggcggc
    30541 cgtcatcagc catcaggcat ccgaatccga acgcctgctg cttgcagacg atgtgctgct
    30601 caatgacggc agcctcaaaa gcctgcgtga gaaaacaatg cgcctgcacg cgttttattc
    30661 agggattttc gcctcaaaac caacacaagg aaaacacaat gactgaatcg cggcaaacac
    30721 gccttcaagt caaatgtccg acctgtcaaa cagcagtagt atggaaaccc gaaaacgcat
    30781 tccgcccctt ctgttcgcaa cgctgcaaac tgatcgactt gggcggatgg gcagacggga
    30841 aatatacggt ttccggccaa acggaaagtt tgccggaaat atccgaaccc gacatggcat
    30901 accgctgacc gccccgcctt cccggcaaac accctgaaag tcaaatgccg tctgaaacaa
    30961 acacgcttca gacggcattt tcattctcaa acctaatcgt tggtatttgc cgttacctct
    31021 tccaatgaag taatgccctg cataactttc aaaataccgg cccggcgcaa atccaccata
    31081 ccctccttat aggcaacgtc caaaatatcc acttccgtac cgttgttcat aatcacacgc
    31141 tgcatttctt cgctgatggg cataacctca tacacgcccg cacgcccctt ataaccctgc
    31201 ccccggcaac ggtcgcaacc gacggcgcgg taaagtttcc aatcttttgc aaggtcctca
    31261 tcggtgaagc cgacttcctt caaagcagag gcagacgggc gttccacttc ctgtttgcag
    31321 ctcgaacaca gcctgcgtaa aagacgctgc gccataatca ggctgaccga actggcaata
    31381 ttaaacggcg cgacacccat attcagcata cgcgacaacg tcgccggcgc attattggtg
    31441 tgcagggtgg aaaacaccat atgccctgtt tgtgccgcct taatcgcaat atcggcagtt
    31501 tccaaatcac gaatctcacc gaccataatg atgtccgggt cctgacgcag gaaagacttc
    31561 aaagcagcgg caaaagtcag gccctgctta tcattgacgt taacctgatt gatgcccggc
    31621 aggttaatct cggcagggtc ttccgccgtt gcaatattta ccgactccgt attcaaaata
    31681 ttcaaacagg tatagagcga caccgtctta cccgaacccg tcggaccggt taccagcacc
    31741 atcccgtagg gacggtgaat cgcttccaac aacaattttt tctgaaacgg ctcaaaaccg
    31801 agctggtcga tgttcaaaga cgcggcatcg gaattcaaaa tccgcatcac gaccttttcg
    31861 ccaaacagcg tcggcaatgt gctgacacgg aaatcgacag gcttgccgcc cttttgaaag
    31921 gtcagctgca tcctgccgtc ctgcggtatc cgtttttcgg aaatgtccaa acgcgacatt
    31981 accttaatcc gtgaagcaag ctgccccctt accgcaatgg gcggctgaac cacctcgcgg
    32041 agctgcccgt ccacacggaa acggatacgg gcattgtgtt cgtaaaactc gaaatggatg
    32101 tccgatgccc cgctgcgcaa ggcatccgac aaagtcttat ggataaacct cggaacaggg
    32161 ccgtcttctg cctcctcgtt gtcgatatac agggtgtggc tttcctcttc ctcctgcccc
    32221 tccccaagct cctgaagcag cgatgtcgaa cgcgaaccca cccaatcgag caaacccgcc
    32281 aactggtcat cctcgacaat gaccaactca acctcaatcc ctgcggcaga aacggttttc
    32341 tgaatttgcg gcatctgtgt cggatcggaa accgcaaaaa atactttgtc gccccgacgg
    32401 aaaaccggca cacagtggaa ctccaccatc tgctcctccg tcaacacccc catcagcacc
    32461 ctgtggcgcg gataatgacg caaatcaaga atcgaataac tgaacaccct cgcaatcaat
    32521 gccgcaagcg acttgggcga aatgacaccg tctgaaaaca gcatcggcaa cacttcctta
    32581 cccgcctgcg actcattgta gtaatgctcg gcctgctcaa cagtaaccac ctggttttga
    32641 accagaatcc tcagcaaacc tacgctcata cgaccttatc cccaaattta ttcattgtta
    32701 tacctgtaca gcttttatag tggattaaat ttaaaccagt acggcgttgc ctcgccttgc
    32761 cgtactatct gtactgtctg cggcttcgtc gccttgtcct gatttaaatt taatccacta
    32821 tatcataaaa caacagattt ccaagccgga acatctttta cgaagcctga aaatcatttc
    32881 atgattctac cgtcctaaag gtcggttttt caagcaggaa gaaaaagttt tcagatggca
    32941 aaaaagccct ccaacactga aaggccttat atcggaaact tcccgcaaca cgggaaacag
    33001 acaaatgaaa tcgtcaaacc tcgccaacag gaatcgaacc tgtattttac gcttaggagg
    33061 catacgttct atccgttgaa ctatggcgag ccgaaatgaa aaggagattt taaccctttc
    33121 cgacgacaaa agacaagatg ccggccgcaa tcagcggccc gacagggata ccgcccataa
    33181 atgccacccc gataaccgtc ccgattaaca gccctgtaat taaaacaggc tgctgtccca
    33241 tcataaggca cgccgcgtcc cgccagccaa gccacgaaaa taccgataaa aacggcggat
    33301 atcattttaa aattcaaaaa ttcggcaacg ggaggaacct gcgcctttcc tgaaaccaac
    33361 ggactcaaaa cccctatggt caaaagaatg ataccgagat tcaacccgtg cttctcgacc
    33421 aacgggacaa actgtatcaa tgccgtctgc tgcatcagca gcaatatggt tgccgagatg
    33481 gtaatcgaat tgttgttgct gaccaccccc aacagaatca gcgtaaccag aaacagggga
    33541 acaaaactga aattcatcgc ctcaagacac cctgaccaaa gattccttcg ccagctgaat
    33601 catggcatcc ttcacttcgc tgtcgggcag ggcatccaag gcggtaacgg cacaatcgac
    33661 tgctttgcgc gcctcgccta tcgaatatgc caaagcatcc gaacggacga cataatcgtg
    33721 gattttctca aaatagctgc gatctgcatt ttccaaagca gtacgcacat cgttcgcaac
    33781 ctgttcggaa ccctgacgca tcagataaat caaaggcaaa gtcggttttc cttccgccaa
    33841 atcgtcgccg acgtttttgc cggtttcttc ggtttcgcca gaatagtcca gcacatcgtc
    33901 aataatttgg aatgccgtac cgacatacat accgtagtct ttcaacgcct gttcgtgttc
    33961 gggggaagcc ttgcccaaaa ttgcgccgac ttgagcggca gcttcaaaca attttgccgt
    34021 tttatattgg atgacttgga tatattgttc ttcggtaatg tccgtattgc cgatgttcat
    34081 cagctgcatg acttcgccct cggcaataat gttggttgca tccgccatca cttccaaaac
    34141 gcgcatactg cccgaggcaa ccatcagttg aaaggcgcgc gtgtataaaa agtcgccaac
    34201 caacacagcc gccgcattgc cgaacagatt gtttgccgtt gcccgcccac ggcgcaaatc
    34261 gctttcatcg acgacatcgt cgtgcaggag ggtggaagtg tggataaact cgaccatcgc
    34321 cgccagcgca tacagtttct cgtcatcata accgaccgcc ttacccgcca aaatcgtcat
    34381 aatcggacgc aggcgtttgc cgcccgcgct gatgatatat gtaccgattt gcgaaatcag
    34441 tgcgacatcg gattgcaccg cacggttgat gacttcattg actttggcaa ggtcttcagg
    34501 cagatgtcgc tggaaatagg gcagattctc gagcatggca gactctcagg tttacgtttc
    34561 cgtcaaacgg cagataccgc ttgaaagctg attcaaatag cgggcaatta tatcagcaaa
    34621 gccatcaggg tacatctttt cagacggcat atggttatcc gaaccgcgca aactttgaca
    34681 aaacaggcaa ataacaatac aatgccgaat ttcgcacaat ggtgtgcgga tattaaccat
    34741 aacccttatg gagttgagta tgtacgcggt cgtaaaaacc ggcggcaaac agtataaagt
    34801 ttccgtcggc gaaaaattga aagtagaaca gataccagcc gaactcgaca gccaaatcga
    34861 actgaccgaa gttttgatga ttgctgacgg cgaatctgta aaagttggcg caccctttat
    34921 cgaaggtgca aaagtaacgg ctaaagtagt ggcacacggt cgtggcgaaa aagtccgcat
    34981 cttcaaaatg cgccgccgca aacactacca aaaacgccaa ggccaccgcc aaaatttcac
    35041 ccaaatcgaa atcgtggcaa tcgcctaacc ttaaaactta ggagcattac aaatggcaag
    35101 taaaaaagca ggcggcagca cccgcaacgg tcgcgattca gaagccaaac gcttgggcgt
    35161 taaagcctac ggcaacgagc tgattcccgc aggttccatc atcgtacgcc aacgcggtac
    35221 caaattccac gcaggcgaca acgtaggtat gggcaaagac cacactttgt tcgccaaagt
    35281 tgacggttat gtcgaattca aaaccaaagg cgcgctgaac cgtaaaactg tcagcatccg
    35341 tccttacacc ggttctgaag aataatccaa ccgatacctg aagccgcatc ttttcacgat
    35401 gcgg
//
LOCUS       DAWFJR010000013        33766 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWFJR010000013 DAWFJR010000000
VERSION     DAWFJR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33766
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.013"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(59..1369)
                     /locus_tag="WGR04_000541"
     CDS             complement(59..1369)
                     /locus_tag="WGR04_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ0794155.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(1508..2686)
                     /gene="ftsZ"
                     /locus_tag="WGR04_000542"
     CDS             complement(1508..2686)
                     /gene="ftsZ"
                     /locus_tag="WGR04_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ0794156.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(2805..4049)
                     /gene="ftsA"
                     /locus_tag="WGR04_000543"
     CDS             complement(2805..4049)
                     /gene="ftsA"
                     /locus_tag="WGR04_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ0794157.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(4135..4863)
                     /locus_tag="WGR04_000544"
     CDS             complement(4135..4863)
                     /locus_tag="WGR04_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ0794158.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            complement(4853..5767)
                     /locus_tag="WGR04_000545"
     CDS             complement(4853..5767)
                     /locus_tag="WGR04_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ0794159.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKEKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRNDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVMGVGFADLCIEILKTAHVG"
     gene            complement(5880..7289)
                     /locus_tag="WGR04_000546"
     CDS             complement(5880..7289)
                     /locus_tag="WGR04_000546"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ0794160.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            complement(7443..8510)
                     /gene="murG"
                     /locus_tag="WGR04_000547"
     CDS             complement(7443..8510)
                     /gene="murG"
                     /locus_tag="WGR04_000547"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ0794161.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVQAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(8514..9839)
                     /locus_tag="WGR04_000548"
     CDS             complement(8514..9839)
                     /locus_tag="WGR04_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ0794162.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGLLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(9965..11302)
                     /locus_tag="WGR04_000549"
     CDS             complement(9965..11302)
                     /locus_tag="WGR04_000549"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ0794163.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEYEADFWLERETGRLKQGNEDLIATQDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLEEAVQTAYAQAEACDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(11440..11658)
                     /locus_tag="WGR04_000550"
     CDS             complement(11440..11658)
                     /locus_tag="WGR04_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ0794164.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGHGVRQDRALAQEWLGKAC"
     gene            complement(11785..12867)
                     /locus_tag="WGR04_000551"
     CDS             complement(11785..12867)
                     /locus_tag="WGR04_000551"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ0794165.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAVIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLAAFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            complement(13114..13290)
                     /locus_tag="WGR04_000552"
     CDS             complement(13114..13290)
                     /locus_tag="WGR04_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794166.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(13293..14651)
                     /locus_tag="WGR04_000553"
     CDS             complement(13293..14651)
                     /locus_tag="WGR04_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ0794167.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMVAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(14776..16422)
                     /locus_tag="WGR04_000554"
     CDS             complement(14776..16422)
                     /locus_tag="WGR04_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ0794168.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWRYCVSGGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            complement(16445..17923)
                     /locus_tag="WGR04_000555"
     CDS             complement(16445..17923)
                     /locus_tag="WGR04_000555"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ0794169.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKFWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELVGRLKKDCPDLAVYSYGFSEHADIRIIDF
                     TASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDNVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(17948..19693)
                     /locus_tag="WGR04_000556"
     CDS             complement(17948..19693)
                     /locus_tag="WGR04_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ0794170.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(19754..20017)
                     /gene="ftsL"
                     /locus_tag="WGR04_000557"
     CDS             complement(19754..20017)
                     /gene="ftsL"
                     /locus_tag="WGR04_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ0794171.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVAHQR"
     gene            complement(20014..21000)
                     /gene="rsmH"
                     /locus_tag="WGR04_000558"
     CDS             complement(20014..21000)
                     /gene="rsmH"
                     /locus_tag="WGR04_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ0794172.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDLQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGSIPEQSQRKTSEWQ"
     gene            complement(20997..21452)
                     /gene="mraZ"
                     /locus_tag="WGR04_000559"
     CDS             complement(20997..21452)
                     /gene="mraZ"
                     /locus_tag="WGR04_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ0794173.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            21731..22948
                     /locus_tag="WGR04_000560"
     CDS             21731..22948
                     /locus_tag="WGR04_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0794174.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLI
                     AEKIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLEHGCMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYL
                     PQSHFLLNLGITELLAQTGKTNSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGID
                     WAGFSFGDICHKL"
     gene            23080..23156
                     /locus_tag="WGR04_000561"
     tRNA            23080..23156
                     /locus_tag="WGR04_000561"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23114..23116,aa:Met,seq:cat)
     gene            23164..23239
                     /locus_tag="WGR04_000562"
     tRNA            23164..23239
                     /locus_tag="WGR04_000562"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23197..23199,aa:Ala,seq:ggc)
     gene            complement(23530..24351)
                     /locus_tag="WGR04_000563"
     CDS             complement(23530..24351)
                     /locus_tag="WGR04_000563"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ0794175.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LIPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFVAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(24384..25028)
                     /locus_tag="WGR04_000564"
     CDS             complement(24384..25028)
                     /locus_tag="WGR04_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0794176.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEREDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRSVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYGIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(25031..25894)
                     /gene="ftsN"
                     /locus_tag="WGR04_000565"
     CDS             complement(25031..25894)
                     /gene="ftsN"
                     /locus_tag="WGR04_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0794177.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDATKEAEQSDAEKAADKQPIADKA
                     DEVEEKAGESEREKSDGQAVRKKALTEEREQTVGEKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTPDKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHE
                     VASLIRSIESK"
     gene            complement(26000..27496)
                     /locus_tag="WGR04_000566"
     CDS             complement(26000..27496)
                     /locus_tag="WGR04_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ0794178.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECQVPQMPFEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQARIQKEAQEAL
                     GNLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(27510..27830)
                     /locus_tag="WGR04_000567"
     CDS             complement(27510..27830)
                     /locus_tag="WGR04_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ0794179.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            28313..29839
                     /gene="putP"
                     /locus_tag="WGR04_000568"
     CDS             28313..29839
                     /gene="putP"
                     /locus_tag="WGR04_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ0794180.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            30090..33695
                     /gene="putA"
                     /locus_tag="WGR04_000569"
     CDS             30090..33695
                     /gene="putA"
                     /locus_tag="WGR04_000569"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ0794181.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFLEWSATPTAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSTLAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVAHLINKALAKRNDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSKKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDNVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTELAASGVQAVVEPDSPWASYTADLEGLLLVNGKPETADISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         7950 a         9738 c         8345 g         7733 t
ORIGIN      
        1 ctggcaaaca aaatgccgtc tgaaaacctt tcagacggca tttttaatat cgaatcaatc
       61 agcccaaata ccagaatttc agtgcccaca atacggcaac aatccatacc ataggcggaa
      121 cgtctttggt gcggcggcat aaaagtttaa ccacggcata actgatgaag ccgaaagcga
      181 tgccgtctgc aatcgaataa gtaaacggca tgaaaacaat ggtcaggaac gcaggtgcgg
      241 cttccgtcat atcgtcccaa tcaatatccc gggcactgcg gagcatttgt gtgccgacat
      301 aaagcagggc gggcgcggtg gcaaaagcgg gaacactttt cgccaaaggt gaaaacatca
      361 ggcaggcgag catcaatacg ccgacggtaa ccgccgtcag gccggtccgt ccgcctgccg
      421 atacgcccgc cgcgctttcc acataaggcg tggtggaaga agtacccaaa gccgcacctg
      481 ccacaatggc ggtagagtct gcaagcagtg cgcgtttcaa gcggggcagc ttaccgtcca
      541 ccagcagccc ggcacggtgg gatatgccga ccagcgttcc ggtactgtca aatagatcga
      601 ccaagaagaa gacgaaaatc acactgacca tgctgacggt aaacaggcct tcaaaatcca
      661 tctgcataaa agtcggcgca atgctcggta cttcgccgat gatgccgtga aattcattca
      721 aacccatcag gctggctatg acggtaatgg tcaagatggt gatgatgatt gcgccttgaa
      781 cgcggaaatg tcccaatacg accaccatag caaaaccgaa cagtgccaac aacgcggacg
      841 gctgatgaat atcgcccaaa ccgaccaagg ttgccggatt ggcaacgata atgcctgcgc
      901 ctttcaggga aatcagtgcc aaaaacaaac cgataccggc agcaatcgac attttcaaac
      961 ccataggcag tgcgttgacc agcatttccc tgactttaaa aaagctgaac aggataaaaa
     1021 tcagaccgga gatgaacacc gcacccaacg caacctgcca aggcacgccc atacccttaa
     1081 cgacggcaaa ggtgaaatag gcattcagcc ccatccccgg tgcgagtgca atcggatagt
     1141 tgccgacaaa acccataaca aaacagccga tggcagacgc gatacaggta gcgacgaata
     1201 ccgcccccat atccatgccg gtctcgccca aaatcagagg gttgacgata acgatgtagc
     1261 acatcgtcaa aaaagttgtc aaacccgcca tcaactcggt acgcaccgtc gtaccgtttg
     1321 ccttcagctt aaaaatcccg tccaacagtg tttgttttga agtgtccata tctgaaagtc
     1381 tgtatcaagt ggaaaaatct gaatccgccg accgcaaacc gtacgaaatt gcaacatctt
     1441 gaaaaacata cctcccaaac cggacaaacc gattcgggag gcagcaggtt tacgggcaaa
     1501 cagcacatta tttgtctgaa ttgtgttgac gacgcaaaat cgcagggatt tcaaagtcgt
     1561 caagtacgga ctgattgtcg aaatccgcag cggtaaggtt catcgtgcgg ataccgcgat
     1621 tggtgcggat cataccttcg acattatggc tttgctcctg tttggacggg gcaaccgctt
     1681 ctacctccct tgccggaaca aaatcgaccg cgcctttttc tttcagaccg gtagcgataa
     1741 tggtaatccg gatggcatct tcgctcatgg tctcgtcttc agccgcaccg aatttacatt
     1801 ccaaatcggg atgcgcgctt tggttgacga ttttcatgac ttcggacaac tcggacattt
     1861 tcaagcaacc cggagcagtc gtaatattga ccagcacacc gcgcgctccg tccaaggtta
     1921 catcgtccag cagcggactg gaaatggcct ggtcggtcgc catacgcgca cggtcgatac
     1981 cttgggcata acccgaaccc atcatagcga taccgcggtt gctcatcacg gttttcacgt
     2041 cggcaaagtc gaggttgatg atttcgctcg ggcaagttac cacttcggaa atgcctgcga
     2101 ccgcatcgcg caatacattg tcggcggcac ggaaggcttc gcgcatcgtt acgtcttcac
     2161 ccaatgcagt catcagtttg tcgttcggga tgataatcag cgaatcgacg tgttctttca
     2221 actgttccaa ccctgcctgt gcgacatgga cgcgcttacc ttcatatgcg aacggtcggg
     2281 taaccacggc aacggtcaga atgcccaaag acttggcaat ctcagcaaca accggcgcgg
     2341 aaccggtacc ggtaccgccg cccataccgg tcgtgataaa cagcatattc gcaccgcgaa
     2401 tggcttcttc aatggcttcc cggtcttcct gggctgccgc acggccgata tcgggattcg
     2461 cgcccgcgcc caaaccgcgt gtcagattcg tacccaactg gattctcttc gccgcatggt
     2521 tttttgccag agactgcgca tccgtattgg cactgataaa ctccacaccg cgcacattgt
     2581 tggcaaccat gttattgatt gcattgcaac cgccgccgcc caagccgatt actttaatca
     2641 ccgcagggct gactgccgat tctgccacgt cgtaaacaaa ttccattcaa aaactcctgc
     2701 tcgccccatt cagaggacgg tttaaataaa ttattattca ttatataaga agtatcttgc
     2761 tgctggcaaa atacttctca cctgtcaaac ggcaatccac ctgttcagaa gctgttttca
     2821 atccaccgtt tcaatcttgc caacaaaccg ccgccccctt ccctctcttg cactgcaccg
     2881 ttttccggct gcggcaagtt tccttccagc ttgcatgctg catgaagcag cccgatagcg
     2941 gtagaaaaac gcggtgtgcg gacgcggtcg gacaaaccgc ccatttcttg gggtgcaccg
     3001 gtgcgtacag gcaaatcgaa gattttttcg gcaaattcca caatcccggt catcatggac
     3061 acaccgccgg tcagaacgat acccgcattc agcacttctt tggggaaacc cgatttttgc
     3121 agctcgccca gcactacgcc aaaaatctcc tgaatccgtg cactgatgat tgctgccaga
     3181 accttactgg aaacctgacg cgatgtccgg tcacccacgc ccggaacttc aatcatctca
     3241 cccaagcctt ccgtatcgca tgatgccacg ccataatgga ttttaatgta ctcggcggca
     3301 tcgagaggtg ttctcaacga tttggacaaa tctttggtaa tcagattacc accggccgga
     3361 atgacggacg tatggcggat ggcaccgttc atataaacgg caatatcggt cgttccgcca
     3421 ccaatgtcga tgacgcatac gccgaggtct ttttcatctt cagtcagcac cgcctgcccg
     3481 cttgccaacg gctgaagcat gatttgatcg cttttcaagc cgcaccgctc gatacatttt
     3541 tggacattct gcactgccgt acttgcaccg gtaatgatgt gcacccgcgt atccagacgc
     3601 acaccgctca taccgatggg ctccctcacg ccaagttggg tgtcgataat gtagtcttga
     3661 accacggcat cgagaatttt ttgatcgggc gggatattga ttgcctttgc cgtttcaatg
     3721 gcgcggtcga tgtctgcctg cgtgacttcc ccgtctttaa ttttaaccac accttgcgaa
     3781 ttgagactgc ggatgtggtt gcctgcgata cctgtggtaa cgtgggtaat tttggtatcc
     3841 gccatcagct cggcatcatt gaccgcctgc ctgatggctt ggacggtggc atcgatattg
     3901 gttaccatgc ccgcgcgcaa gccccgtgaa ggagcctgcc ccaaaccgac gatgttgatt
     3961 ttgtcgtcat cttgaacttc cccgatcagt gcgaggactt tagacgtacc gatatccagt
     4021 acgctgatgt atctttgctg ctgttccatt gttcgtctgc tcttaaaact gattgaaatt
     4081 tgcgtcgcac catttcagac ggcacggccg taatctgtcc gatacctgtt cccactattc
     4141 ttcggattct ttttcgggta aaccgtcgga agcatagcgg actgaaaatc cgtccttata
     4201 cctcatatcc acataggata accgattttt atttttacgc aacagatgct gccacgcttc
     4261 ggtaaaaagc cggaggcgtt tcatctcgtt ttcccgtccg agcctgacgg tgatgccgtt
     4321 gtccaaaacg acaatccacg ccgaacgtgc cgtataggtc atctctttga tgcccaaacc
     4381 ctgttttgcc aaaacagtcg aaaattcgtc ataacggcgg agcatttcgg cagacgttcc
     4441 ttccgcgcct ctgaataccg gcattccggg tctgtccaag cgggcttcaa aaacattgcc
     4501 ttcgccgtcc accaaggcat ggtcgcccca acgcgcgacc ggcttgcgct cggtcaggac
     4561 gacctcaacc gtgtcgggaa aacggcggcg caccatgacc gacgcaatcc acggataccg
     4621 gcggtaggcc tcctgtgcgc cattgatgtc cgtcctcaaa atattcccat ggatgtattc
     4681 tttcgccaaa ctgcccaatg ccttcttatc ggaataaacc aggttgccct tcagcgacac
     4741 ctgcttgacg ggcagatgat tcgaattgta aaaccaaacc agcccggacg cagcaagcag
     4801 catcgccatc atgacaagca gccagcgcgt cagccgttcc atcgcttcgg cattatccca
     4861 catgtgcggt cttcaaaatt tcaatacata aatcggcaaa acccacgccc ataacggcag
     4921 cggatttcgg tactaaacta tggccggtca taccgggcag ggtgttgatt tccaacagat
     4981 agagtttgcc gtcggtatct ttgaggaaat cgacgcgcac gcagccttcc gcaccgattg
     5041 cctgcgcgcc gcgaaccgcc agttcgcgca tcaggctttc ttcggcttcg gtcagatctt
     5101 ccgaaggaca ttgataaatg gtgtcgttgc ggttgtactt ggcttcgtag tcataaaact
     5161 cggttgcggg aatgatgtgt atgccgggca gccctttgcc gttcaggacg gggcaggaat
     5221 attcgccgcc gccgataaaa cgttcggcaa tgatttcgcc ctgaaggtgt ttcaattctt
     5281 cgtaaacgct tttcagacgg cctttttctt tgacttttac cacgcctacg ctgctgcctt
     5341 cggccgccgg tttcacaaac atcggcaggc ccaatttttc ttcgacggca tcgaaatcag
     5401 tgtcgtcgtg caggacggcg aactcgggaa cgggcaatcc caatgcctgc caaatcagtt
     5461 tgcagcggta tttgtccatg ccgatggcgg atgcggcgac accgctgccg gtatagggaa
     5521 tgcccaacag ttccaatgca ccctgaaccg ccccgtcttc gccgtaagta ccgtgaagga
     5581 tgttgaatgc cgtctgaaaa ccttgtgcct tcaattcaga caatggggtt tctttaggat
     5641 cgaaggcgta tgcgtctatg cctttgcttt ttaaagcatt caaaatggcg gtgccgctgt
     5701 ccagcgagat ttctcgttcg ctggaaaacc cgcccatcaa tacggccact ttgccaaaat
     5761 tctgcattgt ttttgttctt tcctgattgc tttatgcttg ttgccagagg tcgtctgaaa
     5821 cctgatttgc ggtttcagac gacctttata tgatgttccg tctgtcaggc gggtgtgcct
     5881 caaatctgtt tcgacaattc cagcagcgcg gcggggacgc ggttgatgct tcccgcgccc
     5941 atattcaaca cgatgtcgcc gtcctgcaaa acgttcaaca gcatttcggg cagatcggca
     6001 acgttttcgc agtaaatcgg ctcgagtttg cccaacacgc ggatggcgcg ggcaagagcg
     6061 cgggaatcgg cggcggcaat cggctcttca ccggcggcat aaacttcggt cagcaccagc
     6121 gcgtcaacgg tattgaggac tttggtaaag tcttcaaaca aatcgcgcgt gcgggtatag
     6181 cggtgcggct ggaaggcgag taccaaacgt ttttccggat acgcgccgcg tgcggcggca
     6241 agggtcgccg ccatttcgac ggggtggtgt ccgtagtcgt ccaccaagag cgcggtcccg
     6301 ccgtttggca acttgatgtc gccgtatttt tggaagcggc ggccgacgcc ttcaaagccg
     6361 agcaagcctt tttggatcgc ttcaaccgat gcgccgactt ccagcgccac gccgatggct
     6421 gccaatgcgt tcagcacgtt gtgtctgccg ggcatattca gcacgacttc aaacgacccc
     6481 tgctcatgtc ctttcatttg aacatggacg gtgaatttca tttgcgcgcc gacgttttcg
     6541 atgtcggtgg cgtagatgtc ggcggtatcg tccaaaccgt aagtagcata aggtttgctc
     6601 actttgggca aaatcgcgcg gacgtgttcg ctgtcaatac acaaaaaggc tttgccgtag
     6661 aagggcatac ggtggatgaa atcgataaac gcctgatgca gtttttcgac gctgtgcccg
     6721 taggtatcca tatggtcttc gtcgatattg gtaacgacgg acataatcgg tgtcaggtgc
     6781 agaaaggatg catccgactc gtcggcttcg gcaacgatgt attcgccttt gcccaagcgg
     6841 gcgttggtgc ctgcggcgtt gagtttgccg ccgataacga aagtcgggtc aagtcctgcc
     6901 gcgccgagga tggaggcggt caggctggtg gtcgtggttt tgccgtgcgt gccggcaatg
     6961 gcgatgccgt cacggaagcg catcaactcc gccaacatca gggcgcgcgg aataacggga
     7021 atttgctgct ccaacgcagc gacaacttcg ggattttctt ttttgacggc ggtagaggta
     7081 acgacgacat ccgcaccgtt aacgtgttct gcggtatggc cgggataaac ttgaatgccc
     7141 aagctgccca aatgctcggt agcggcattt cgcgcctgat ccgaaccgga aactttaaaa
     7201 cccaaattgt gcaagacttc ggcgataccg ctcatgccga cgccgccgat accgacaaaa
     7261 tggatgttgg tcactcgatt tttcatcata atgttgcgtt ccggtggatt tcgatgcgta
     7321 aaggcgttat tttaaagggc tgaccgtttg cgcgccatag ttttctgaca aatatatagc
     7381 ggattgaaat aaaaaacatc catgctgtct gaacggcttt tcagacggca tggttcggca
     7441 gtttacgccg cacacgcaat cgcggcttcg gcaacgtcgt ccgcgctgtg cggcaatgcc
     7501 aaagtacggg cgttttctgc ccatttgaga catttttcgc ggttcaggct gccgaggatt
     7561 tcggcgagtt tttccgccgt caactgggtt tgcggcaaca gcaatcccgc ctccgcctgc
     7621 accataaaac gcgcgttggc ggtttgatgg tcatcaacgg cgtgaggata cggcactaac
     7681 aacgcaccca atcccgccgc cgtcaactcg gcaatcgtca gcgcgccggc acggcaaatc
     7741 accaaatcgg catcgcggta ggcggacacc atatcggtaa taaattccac gcattccgct
     7801 tgcacgccca gcgcgtcgta atccgcctgc aagctgccca gcttgccccg tcccgattgg
     7861 tggtacatct gcggacgcgc attgtcgggc agcaaagcca atgcctgcgg tacggttttg
     7921 ttcaaaacgt ccgcgcccaa actgccgccg accaccaaaa ttttcagacg gccttcacgc
     7981 ccttggaagc gttcggcagg cacgggcagg ttgctaatat cggcgcggac ggggttgccg
     8041 accaagccgc cttcgtggct gaacgctttc ggaaaagcgt acaacacccg cttcgcccag
     8101 cgcgacaggt ggcggttgga caaacctgcc acggcgtttt gctcgtgaat cacaatcggc
     8161 acgcctaata gcttcgccgc caaaccgccg gggaaggtaa cgaagccgcc gaagccgatg
     8221 acgcactcga cacggtgttt gcggataatc cgctgcgctt cgcggacggt ttgatacaaa
     8281 gtaaacggca gcatcagctt gcgtttgatg ccgttgccgc gcacgccttt aatcgccagc
     8341 gtttccaagc gtatgccgta ttgcggcacg atacgctctt ccatcgaatc cttgctgccc
     8401 agccaaatca catgatggcc gcgcgcgcgc aatgaatccg ccaccgccag cgcggggaaa
     8461 atatgtccgc ccgttccgcc cgccatcagc ataaaggttt taccgcccat gatttactcc
     8521 acccgataac cgcgcatttt ccggcggttt tcataatcta tacgcaacag cagcatcatg
     8581 ctgatcagca tgaaaaagac tgacgaaccg ccataggaca tcaacggcag cgtcagacct
     8641 ttggtcggca aagcaccgat gttcacaccg atattgaaga aactttggat accgatccaa
     8701 atgccgatac ccgaagcgat ataggcgttg aaagtcaaac ccaaatcgcg cgactgctta
     8761 ccgatggaaa acgcccgcac caccagccag ccgtaacaga atatcagcac acacataccg
     8821 aagaaaccga attcttcggc gatgatggca aaaataaaat cggtatgcgc ttccggcaga
     8881 aagccgcgtt tgctcaaact cgcacccaaa cccataccga accactctcc gcgcccgatt
     8941 gccatcagag agtgggtaag ctggtagccg gcaccctgcg ggtctttcca cgggtccaaa
     9001 aatgccacta cccgctgcac acggtaggga gcggcggtaa tcatcagcac catcccgccc
     9061 aagacgctgc ctaccaggac gaaaaaatat ttccacggca atcctgccaa aaacagcatt
     9121 ccaacggcaa tgacggtaat gacgacaaac gaaccgaaat ccggctgtac cattatcagc
     9181 accaaaccga acgccaccag cataatcggc aggatgatcg cccggaaacg gccgtacatt
     9241 tctaatgttt cacgacgtgc ctgcggattg gtggcggaca tgatcagatt ggccgtcccc
     9301 cgccaaatcg actgccaacc caaactttcc atgctgcgca acacttcttc acggcgcgtg
     9361 aacaggcttg ccaaataaag gatgaccgcc agcttgaaca gctcggtcgg ctggaaattc
     9421 aacggaccca aaggtatcca acgggtcgcg ccattgattt cgcgcccggc aatcaatacg
     9481 actaccagca acaggccgga tagggcaaaa atccacggca caagccgccg ccatgtcctc
     9541 atcctgcaaa gaaaccataa caaaccgctc gctatcaagc cggcaacgac gaaccccgcc
     9601 tgtctggtca aatagaaaaa ctgatcgccg ccttcttttg atgccaaata cacagaagcc
     9661 gaataaatca tcagcaggct gaacgccgtc atcagcacca ccatccacaa aagcggcgcg
     9721 tcgaatttcc tgccgtcgcg cacaatcggc ctgtcgagca gcagagtgtg gacaccgtcg
     9781 cccactttta ccaatacttc cgaaatcttc aaaaaaacca cctgccagtc tgtttgcacc
     9841 tgccgcaaag ggcaaaaatt tcagacggca gacaatgccg tccgaacata cgatacatcc
     9901 caaatcggta ttctaaatct ttacttgccg cccaacaatg acggcgtttg catttcagac
     9961 ggcatcacaa agccttaaac gcttcgataa acacttccga acggtgcgcg tagcctttaa
    10021 acatatcaaa actcgcacaa gccgggctga gcagcacgat atcgcacgct tcggcttggg
    10081 catatgccgt ctgaacggct tcttccaaag tggcgcagtc ggtcatattc agaccgcagc
    10141 cgtccaaatc gcggcggatt tgcggcgcat caacgccgat caggaacacg ccttttgcct
    10201 tgccggcaag cgcgtcgcgc agaggcgtga agtcctgccc tttgcccatg ccgcctaaaa
    10261 tcacgaagag cggattttgc aaaccggcaa tcgcggcggc ggtcgcaccg acgttcgtgc
    10321 ctttgctgtc gtcgatgaac accacgccat ttttctcgcc gattttttcc acgcggtgcg
    10381 gcaggccttg aaaggttttg acgtgttcaa gcaatgcttc gcgcggcaaa ccgacggctt
    10441 cgcacaaagc cacggcagcc atgacgttgg cggcgttgtg caagccttgc agcggaatat
    10501 cttgcgtggc aatcaaatct tcattgcctt gtttcaggcg acctgtctca cgttccaacc
    10561 aaaaatcggc ttcgtattcc aacgaaaacc atttcacctc gcgcccggcg cgcttcatcg
    10621 cacgacagaa cgcatcgtcc gcattcaaaa cctgcacgcc atcgccacgg aaaatcttgg
    10681 ctttggtatg cgcgtagtcg agcaagtcgt cgtagcggtc gagatggtct tcggaaatgt
    10741 tcagcaccgt cgccgcagtc gggcgcaggc tttcggtgtt ttccagttgg aagctggaaa
    10801 gctccaacac ccacacgtcc gcctttttgc cttcgcgctg ccattccgcc tccaaaaccg
    10861 gcgtgccgat attgcccgcg ataacggtat ccagcccgca cttgatgcag agatagccga
    10921 ccaggctcgt taccgtggtt ttgccgttgc tgccggtaat cgcaattacc ttgtcgcctc
    10981 ggcggttcac aatgtccgcc agcaattcga tgtcgcccaa cacgcgtccg ccgttttgct
    11041 tgaacgcctc aatatccggc tgccgctcgc tgatgccggg gctgagtgcc agaatatcga
    11101 aaccgttgtc cagcgcatct ttcagacgac ccgtgtaaaa caccagcccg tcaaacatct
    11161 taccgatttg cgacacgcgc tccggtttca gctccgcatc atacgcggca acctccgcgc
    11221 cgtttttgcg cagataggca atcatggaaa tacccgtacc gccgagtccg gcgacgagga
    11281 tttttttgtt ttgaaaagtc attttggttt gtcctaaaac aaatcatatt gagcaggaga
    11341 tgtccgcccc tgcccaagcc gctttcagac ggcatcgcga gctgttcaat aacccgcctt
    11401 caggcgttgg tcattgtcgc agccgtcttg gtttccgttt taacaagcct tgccaagcca
    11461 ttcttgtgca agggcgcggt cttggcgcac gccgtgccct ttgtaataca ttgcacccaa
    11521 attgtattgg gctgctgcat ttccctgtgc tgccgcctgc aagttttccc gaaaatccga
    11581 tacgtcatcc gcccacaccg cttggttcaa gcccaaggca atcagggcgg cggcaagcca
    11641 tttgactgtc tgtttcatgt tttctctcct atttcggtgt ggggcggttt ttcagccacc
    11701 gttaacgata ggacgggaca gattgccgcc gcaggtttat tgcgcgttca aatgccgtct
    11761 gaaaggtgtt cagacggcat aggttcagcg gattttgagg gtactcaaac cgatcaacac
    11821 caagacgatg gtaataatcc aaaagcggac gacgacttgg gtttctttcc agcctttttg
    11881 ttcgtagtgg tgatggatgg gcgccatcag gaagatgcgt tttttggttt tcttatacca
    11941 gccgacctga agcataacgg atacggcttc tacgacaaat aatccgccca taatgacgag
    12001 gacaaactct tggcggacga tgacggcgac ggtaccgagc gcggcaccca atgccaatgc
    12061 accgacatcg cccataaaga cttgcgcggg ataggcgtta aaccacaaga aaccgaggca
    12121 cgcgccgcac atggcggtac agaaaatcac cacttcgttt gcgccggcaa cgtaaggtaa
    12181 ttgcaggtat tgggcaaatt gtgagtggcc gctggcatag gcgaaaatgg cgaggccggc
    12241 agcaacgagg acgacgggga aggcggcaag gccgtccaag ccgtcggtaa ggttgacggc
    12301 gttggatgtg ccgacgatgg tcaggtaaga caacaccaaa aagccgacca cgcccagcgg
    12361 cagggcgatt tgtttgaaga acgggacaat caaaatattg ttggcggaat tggcggcaag
    12421 gtaaaacaat gccaaaccgg cgataacggc aacgcttgac tgccacacca ttttgaattt
    12481 ggcggacacg ccgttggggt ctttatagac gactttgcgc cagtcgtcgt aaaaaccgag
    12541 cgcaccggtg gcaagcagta cgcccaagag aatccagata tacgggttcg cccagttgcc
    12601 ccacaacagg gtggacacgg taatggcggt cagaatcagc gaaccgccca tcgtcggcgt
    12661 gccgtttttg acgaggtggg tttgcgggcc gtcggtacgc actgcctgcc cgcatttgag
    12721 cgcggtcagc ctgcgtatcg tccacgggcc gaacatcagg gaaaacgcca aggcggtcaa
    12781 cgccgccatg acggcgcgga atgtggtgta ttgaaaaata ttcagaccgg ttaaccagtt
    12841 gctgaaatgt gcgagccata aaaacatggg gcttcctttt tttgttttgt cgttatagtg
    12901 gattaacaaa aaccagtacg gcgttgcctc gccttagctc aaagagaacg attctctaag
    12961 gtgctgaagc accaagtgaa tcggttccgt actatttgta ctgtctgcgg cttcgtcgcc
    13021 ttgtcctgat ttttgttaat ccactataca tcgggcctgc gcccgtttga ggttggcttg
    13081 ccgcccggca aggtttcgga cggcttttgc agattaacgt gcagggactt tctcaatcac
    13141 gcaaatcggg taactccccg gatttttgcc gccacccaag tctaaacata aatcttcata
    13201 aagatacgcc tgcgtctgca ttccggcata aaacgcgccg accagcgcgg caatcaacaa
    13261 taaggcttta aaaaaacgtc gacttttcat tttcacttat cctccaatgc ctcgaccact
    13321 tcttccatct gcataaagcg cgaacctttc accaacacgg tggcgcgttc gggcaaatcg
    13381 tggcgcaaca cttgaatcaa cgggtctttg gcggcgaacc acaaaccgtc cgcgccaaat
    13441 ttttccgccg cttcgacgct gttgtcgccg acaaaataag ccgcttcgat gccttggtcg
    13501 cgagcatacg cgccgacttc ggcgtgcatg gcggcggctt cgtcctcacc cagttcgccc
    13561 atatcgccca tcacgaaaat acgcggcgca ggcatacgcg ccaacacgtc aatcgcagct
    13621 ttcatgctgt cagggttcgc attataagta tcgtcaatca gggttgcgcc cttgattccg
    13681 gatttgacgt tcagacggcc tttgatattg ctgaagcctt tcaaaccttc cgccacatcg
    13741 ttcaaactca aacctgcagc caaagacagc gcggcggcag cggcggcgtt gtggacattg
    13801 tggcggccgg gaacaggcag caccacggcg gcgcgttcgt cgccgcacac caaatcaaat
    13861 tcgcacgaca acggtttcag cacgatattt tccgcgtgga catcgccgct atcgatgccg
    13921 aaagtgcgcg tattcaaatt aagcgttgcc gttttgaaga cagccatatt ggcatcttct
    13981 tgaggaatca gtgccatgcc gtctgaacat aagccttgat aaatctcgct tttcgctttg
    14041 gcaatatcgc ccactccgtc gaaaccgcag ccgacatggg cgcgcatggc gttgttgacc
    14101 aatgcggcat cgggtttggc gatttgtgtc aaaaccgcca gttcgccaaa atggttcata
    14161 cccatttcaa tcacggcata gcggtgtttt tcgtttaatt tcaacaaagt caacggcaat
    14221 ccgatgtggt tgttgaagtt gcctgccgtc gccaaaacgg cattatcgcc gaaacggcgg
    14281 cgcaataccg cagccaacat ttccttcacc gtcgtcttac cgcccgaacc ggtaataccg
    14341 aacacaaacg ggttcacatt ctcgcgccac gccttcgcca ccatttgcaa cgcggtaagc
    14401 gtgtcatcga ctttcaacgc gccatccatt gcaacgcaat cttcgcgcga aaccacaacc
    14461 gccgccgcac cagcagccaa tacgtcttca acaaaatcat gcgcatcaaa ccgcccgccc
    14521 gccaatgcga aaaacacatc gcccgcgcgg atgtcgcggc tgtcggttac gatgcgcgac
    14581 acgggtttgc tttcagacgg catcggaagc ttgagggctt ggcagatgaa atttaggtcc
    14641 agtggtttca tatttacttt cgttaatatt cgggcggcgg acacatcggt agcggctgat
    14701 ttttttatcg cctgttttgc tgtggtaaaa cacagattat tttcccattc tcattcggca
    14761 ttttttctgt acgtatcatt ttttagacgt atttttagtc gatttgcctt ttcccgcata
    14821 ccacggcgcg gggtcgtcgg gcagtccgtc gataaaggca aggttattgc cttcgccctg
    14881 cacatcggga acattccccc aaaaatcata gccgtcatcg ggcaactcgt cggtttcgat
    14941 acccgtccaa ctgccgaatc cgcgtaaaaa attaaacgcg ctgcgcctca ctttaatcat
    15001 ttcgcgccgc gtatcatcgg acgaaatttt aaccagcggc acgccgtagc tttgacgctt
    15061 ccacgcacca tgtgtcaacg ttcgctcgcc gcctttaccg acatgcatca aaccgtggtc
    15121 ggaaaagtaa accaaagacc agctttcttt attttcattc aatatcttaa ctgtatcttc
    15181 taaaaattta tcggtttgcg cgatggtgga aacatagcag gatatttttt cagtttgata
    15241 ctgaaaccgc cgcgcatcct tatccaaacg tgtgcaaaaa tcactgtgcg aacccatcag
    15301 gtgcatcaca atcagccgag gcttcgtgcc tgcatttcct atcaaaaccc gtttgaacgc
    15361 cggcaacaaa aggcggtcgc tcaaccccgc gcttttgcca taatcacccc tttgggtaaa
    15421 ccacggataa tcgctgcgta gggcataggt ggaaatttcg ttggcaaaat gccccaacat
    15481 tccttgatta gacagccacg ccgtccgaaa acccgcctgc ttcgccaagc tgacgatgtt
    15541 attgttcggt tctcccggca gccccaaagt ctgcggcagc gaaagattcg tcgcgtgggc
    15601 ggtcgattgg taaccgttta tcaacagccc tttggtctga ctcaaaaaag gcgtatcggg
    15661 caatgggaaa ccgtaaacat tcatataatc cgaacgcgcg ctctcaccga taaccacaac
    15721 ataattttta tacttgggcg caacatgacg gatatgccat gttgacgctt tttttgctgc
    15781 ttccaaaatg tgggcgcgct tggcggcata ttgtgccggc gcggaagcca aatcgtaata
    15841 caggtcgaaa atattcaaca acaggccggc atcaggttct cgcaaatctt tatcgctggc
    15901 gattttatcc atcaccgcgc aggacaaaat caaagtcaat aatatagtca gccatatttt
    15961 gctgcggcgt ttatagtttt ttacgtcagc aaataccccc cccgatacac aatatctcca
    16021 aactgtcaag ccaaaaatga aaaataatgc ctgcacaaaa taaagcgacc cgggaagatt
    16081 gccgacaaat tcacgcgcct cggcaggatt gctttccaat atcgaaccga ctatctgata
    16141 agacggcgca ccatacaacc agccgaccgg caaatatagg gcggttgtgc cgacgtaaat
    16201 cagtaaaaca actgaagaaa cgcgcgggaa accgcgtgcc aataataata aaaatacaac
    16261 cgaagccaaa gcacccacgg cagaataacg gtagccgtat tcatattcca agtgataacc
    16321 tgatgcgatg gccgcgccaa aacaaaaggc taaaagcgcg caaatgccca atgtattttt
    16381 taatgtctga tccatatttt ttattcgggc ggattgtttc atacgatgcc gtccgaatat
    16441 cccattattt acgagttaac aaagcctgtc cgacgatttc aagatcggaa aaacggtgct
    16501 tcacgccttg tacatcctga tagttttcat gccctttgcc ggcaatcagg atgatgtcgt
    16561 ttgcggcggc ttgttcaacc gcataacgga tggcggcggc acggtcggct tcgacgcatt
    16621 cgggcgcggg aacggcaggc aggatgtcgt tgatgatgtc gtgcggattt tccaaacgcg
    16681 gattgtcgct ggtgacgacg actttatccg cgccctgtac ggctgccgcg cccatcagcg
    16741 ggcgtttgcc gcgatcgcgg ttgccgccgc aaccgaatac gcaccacaaa gccgcaccct
    16801 gcggtttgat ttcctgcaag gtggcgagtg ctttttccaa tgcgtcgggc gtatgggcat
    16861 aatcgacaac gaccaaaggt ttgccgctgt tcatgatgca gtccatgcgc cctgaagcgg
    16921 gacggatttt tgccagcaca tccaatacat tatcaagcgg atagccgttg gcgcacagca
    16981 aggcgatgca ggcggcgagg ttttgcgcgt tgaaccgtcc gagcaggcgc gtgcggcatt
    17041 tcccttcgcc ccacggggtt tggaatacag ctgctatgcc gtctgaagag gcggtgaaat
    17101 cgataatgcg gatgtcggcg tgttcgctga agccgtagct ataaacggcc aaatcgggac
    17161 agtctttttt cagacgacct acgagttccg cgccgtattc gtcatccacg ttgatgactg
    17221 cgtgtttcaa gccgtgccag taaaacaggc gcgacttgat ggcaccgtag gcttccatcg
    17281 tgccgtggta gtcgaggtgg tcgcgggtga ggttggtaaa gattgcgctg cggaatgaca
    17341 cgccgttgac gcgcgactgg tcaagcccgt ggctggagac ttccatcgcg gcgactgttg
    17401 cgccttgttg acggaaacgg tagagcaggg tttggacatc gacgggggcg ggtgtggtat
    17461 gcgtggtttc ttccaatgca ccccaaaagc cgttgccgac cgtgccgaca atggcggttt
    17521 tttcgcccaa caaatcggca gcttgcgcca gccattgcgt gatggaggtt ttgccgttgg
    17581 ttccggttac gccccagaat ttgagaccgt ctgaaacgtt gccgtaaact tgcgccgcca
    17641 atatgccggc acggtgtttc aaatctttga tgccttgatt ggggactttc cattcgggat
    17701 tccacgcaaa tttgccgtcg tcgtcccaaa aaacaaaagc cgcgccgttg gcaacggcgg
    17761 cggggatata actgcgtccg tcggcatatt cgcccggaca ggcaacgaaa atatcgcctt
    17821 gtttgatttg gcggctgtct gaatgcaaca aacgccctgc cgcgtttgca cacgacaggg
    17881 tcgggatgcc ggtttcagcc aaaggggtta acttgctgaa cataaaacaa tctcgttgat
    17941 actcggatta agacggtgtt ttgacggctg cggcggtcag gggcttggtc ggggaaatgc
    18001 ccaagatgtt caggctgccg cccataattt ttttgaaggg cggccctgcc actacgccgc
    18061 cgtaataacc gtgggcagtc ggttcgtcaa tggttaccgc cacaatcaca cgggggtttt
    18121 tggcgggggc aaaaccgata aaggtagcga tgtgtttgtt gtcggcataa cgcccgttga
    18181 cgaacttgcg cgccgtgccg gttttcgcgc cgacatcgaa accgtccacc gcacccgccg
    18241 taccggtgcc gcccggctcg gtcacggaaa ccatcagatt gcgtacctcg cgcgcggtcg
    18301 attctttgaa tatgcgtttg ccttgcggcg caaccgcctg tttttcaaag ctgaccggca
    18361 gcaaaacgcc gtcgtgcgtc agtgcggtat aggcgcgcgc caattgcagc aggctcaatt
    18421 gcaggccgta accgaaagac atcgtcgcct gttcaatagg ccgccacctg cgccaatttc
    18481 tcaacaaacc tgcagtttcg cccggaaagc ccgagtgcat acgcacaccg atgcccaact
    18541 catgatagaa gtcatacatt tcttcggcac cgaaacgcgc agacagtttg cttgtgccga
    18601 cgttggacga tttctgcatg atgccgcgca catccaaaga ggggtaaaca tgggtatcgc
    18661 gcacgggaga cggtccgatt ttataaggct gcgtattcag ccgttcgttc aaatcggttt
    18721 tgcccgcatc caatgctttc gcaatcacaa acggtttgat tgccgaaccg ggttcgatca
    18781 tatcggttac ggcacggttg cgccgctgtt cgctgtctgc ccggccgggc ctgttgggat
    18841 cgtaggcggg cgtattggcc aaggcgagga tttcccccgt gcgggcatcc aaaaccacca
    18901 ccgttccggc ttttgcctga tggtattcga ccgccttgtt caactcttca taggccaagg
    18961 tctgaatcct ctgatcgagg gaaaggatga tgtctttgcc gtttttcggg gctttattgc
    19021 gcggggagtc caagctgtcc acaatattgc cctgccggtc ccgcaaaacg acttccgcgc
    19081 cgtcttcgcc atgcaggctg tcttcaagcg aaagttccaa accttcctga cctttgccgt
    19141 caatatcggt aaatccgatg acgtgtgcaa acaggttgcc catcgggtaa tggcgtttta
    19201 attctttttc aaatacaaag ttttccaaac ccaaggcttt gacctcttcg gcaaccttgg
    19261 gatcgagctg ccgcttaatc cagataaacg acttgccttt ctgttcgagc ttgttcctca
    19321 aaacatcaac cggcacatcg acaagctcgg acaggcgttc caattgtgcg gcagacggca
    19381 tttccttcat ctctttaggc acggcaaaca gggactccgt cggcgcactc aacgccaaaa
    19441 ccgcaccgtt ccggtccgaa accgtaccgc gtgtagccgg caatgtttga gtccgcacaa
    19501 tccggttgtc gccctgttct ttcaaaaagt tatacgttac cgtctgcaga tacagtccgc
    19561 gagcaatcag accggcaaac aagaccgcta ttgccatcag gacgaagctg atccgtccgt
    19621 tactggtcat cggctttttg acctgctctt ctttgggcag catccgaggc ttatattcgc
    19681 tcttaatcaa catttttact tctcgttatt attatcctga cgcaggaatc cgattccggc
    19741 acacaggctg cttctatctt tgatgcgcca ccataaaggt attgcccgaa accggcggat
    19801 ggaggttttg tttttctgcc gccgccctga tcgcttcgtg gttcgccaaa cgcgcctgtt
    19861 gcagcctcat ttgcgcataa tcctgctcca aggcgatttc ctgttttttc gccttgtcca
    19921 aagctgtgaa gttgagcctg tactggtttt gctgcatcac aacggaaaaa gcggaaacgc
    19981 acaccgcaag cagcagaagg aaattcaatt tgttcattgc cattcagacg tttttctctg
    20041 tgattgttcc ggtatcgaac cggcagtccg ctccgccaca cgcaaaaccg cacttctcgc
    20101 cctcggattg gcggcaattt ccgcctcacc cggctttaat gccctgccca cgattttcag
    20161 gggcggctcg ggcaaatccg cttccctgac cgccgcccag cgcggcaggg gcgcgtgttg
    20221 cgaatatttt ttgacaaact gcttcacaat gcggtcttcc aacgaatgga aagcaatgac
    20281 cgccaaacgt ccgccctctt tcagacgaca catgacctgc ggcaatactg cccctacttc
    20341 ttcaagctcg cggttaataa agatgcggac cgcctggaag gtgcgcgtcg caggatcctg
    20401 cccccgctcg cgagtacgga cgttttgtgc cacgatctgc gccagcttgc gggttgtatc
    20461 gattggactt tccgcccgtt gcgcaacaat ggcgcgcgca atctggcggc taaaccgctc
    20521 ttcaccataa ttcttgatta cctcgtgcaa atcctgttcc gacgcaaccg ctatccactc
    20581 tgcggcagac ataccgcgcg tcgtatccat acgcatatcc aaaggggcat cgaaacggaa
    20641 gctgaaaccg cggctgccgt catcgatttg cggggacgaa atccccaaat caaacagcgc
    20701 accgtccacc ttgccgatac ccaaaccgtc caatgccgtc tgaaacgaag caaaaccgcc
    20761 atgcacgaca ccgacccgtt tgtccgaacg cgccagctct tctgccacag caatcgcctg
    20821 aaggtctttg tcgaaaacaa tcaaccgccc cgcatcgccc aaacgcgaca aaatcagccg
    20881 ggaatgccct cccctgccga acgtaccgtc cacatagaca ccgtcttcgc gcacggcaag
    20941 cgcatccacc gcctcattca gcaagaccgt gatatgccgg taactttctg ctccactcac
    21001 aattgcaaat ccgtctgact caactggaag gcaagttcgt caggatcgtc atccaaagcc
    21061 tgaaccatct cagcctccca ctgctcgcga ccccaaagct ccaaacggtt ggcacgaccg
    21121 accaaaacga cttcacggtc gaaatccacc ctcttcctca gtccggcagg aagcagcacc
    21181 cggccggcgc tgtcccattc caaaatttcc gcgttatgca gcaaaagatt ttgaaaccgc
    21241 cgcaaaacag ggttatccgc cacttttaag tttaaaagtt gcgccgcaac cttttcccac
    21301 tccgcaacag ggtacatcaa cagcttgtgt ttcgactcga gcgttaccac tacggcaggc
    21361 gtatagaggc gcgacagaat gtcacggaat ttggcaggaa cagccaaccg ccccttactg
    21421 tcgatgctta attcgtgtgc gccgccgaac atgacatgtc ccaagccgaa atcaaaatca
    21481 caagggtaaa agagacactt tgccccacaa ttccccacca atcgacacta taagaaattt
    21541 taaacactcg gtcaaatcag ggcatgaaaa ctcattaaca tatctgaaat ttttattcct
    21601 tttaaaaaca ataagataaa aaatggcgac aacggcacgg cgggtgcggt acagagtgac
    21661 cgaaccaata aacaactata tatgattaat ttaataatat aaacacaata tatagtatta
    21721 agataaagcc atgacagcac ccgtaccaac gtgtaatatg tcgggaaatc caataaattt
    21781 acacaagcta acacttatca tgcccctccc cctcccctct cccgaagcac ggcaattctc
    21841 gctcaaactg caaaccctca ttgccgaaaa aatcggcaaa cacggcaact ggattccatt
    21901 ttcacgtttt atggaattgg ttttatacgc tccgcaatac ggctactaca ccggcggcag
    21961 ccataaaatc ggcaataccg gggattttat taccgcaccg accctcacct ctctgtttgc
    22021 acagacactg gcacgccaac ttcaagaact tctatcccaa acggcgggca atatctatga
    22081 attcggcgcg ggaaccggac agcttgccgc cgatttgttg ggcagcattt cggacggcat
    22141 cagccgttac tatattattg aaatatcgcc ggagctggca gcacgtcaga aaaacctgat
    22201 tcaagcacgc gcaccggaag catctcaaaa agttgtccac ttgaccgcac ttcccgaagc
    22261 gtttgacggc atcatcatcg gcaacgaagt actcgatgcc atgcctgtcg aaatcgtccg
    22321 taaaaatgaa ggcggctcat tcgagcatgt cggcgtttgc ctagataatg accgttttac
    22381 ctattcggca cgaccgctgc acgacttaca gctatctgcc ttggcttccc tctattttcc
    22441 tcaaacagat tatccctaca ccagcgaact acatccgcaa caatatgcct ttatccgcac
    22501 ccttgcctca agactcgaac acggctgcat gatattcatc gactacggtt ttgatgcagc
    22561 gcagtattac cacccgcaac gcaatcaagg tactctaatc ggacactacc gacatcacgt
    22621 tatccacaat ccttttgact tcatcggatt ggccgacctg accgcgcatg tcaactttac
    22681 cgacattgcc caagccggga cggatgccgg attagatttg ataggttacc ttccccaatc
    22741 ccatttctta ttgaacctgg gcattaccga gctattggca cagacgggga aaacgaattc
    22801 ggcagcctat atccgtgaag ctgctgccgt tcagaaactg attgaccagc acgaaatggg
    22861 cgaactgttt aaagtcatcg cattcggaaa aaatatcggc atcgactggg caggattcag
    22921 cttcggcgac atctgccaca aactctaacc ctcatgccgc ctgaatccgc ttcagacgac
    22981 ataaactttt taacatttaa aaacagtcaa ctaattcaaa attaaaaaat acggcttgtc
    23041 aaaaaaacag aaaaacatat aatagcgtct tcacgaaacg gcgaattagc tcagtcggtt
    23101 agagcagagg aatcataatc cttgtgtccg gggttcgagt ccctgattcg ccaccaaatt
    23161 ttcgggggta tagctcagtt ggtagagcgc ttgcatggca tgcaagaggt cagcggttcg
    23221 atcccgctta cctccaccag ataaaaaagc acagaccgta aaaaggtatg tgctttttta
    23281 ttgcctgatt gccggcaaat aaagaataaa ccactgcctt caaaacaggc aatcgacttt
    23341 aaacctatcg ccccgcctgt cctgatttta tagtgaatta aatttaaacc ggtacagcgt
    23401 tggctcacct tgccgtacta tctgtactgt ctgcggcttc gtcgccttgt cctgattttt
    23461 gttaattcac tatatcagcc cgccagacaa acccgacccg aataatgtct tcaggtcggg
    23521 tttatggttt cattcccaac ttatccagcc tgacagccac aatataatga tggcaatacc
    23581 aaaaacaatg cggtaatagg caaaaggaat ataatttttc ttggaaacaa acctcagcaa
    23641 cgctttgacc gctaccaagc ctgaaacaaa ggcagcaaca aagccaatca aaatcaaacc
    23701 gacatcatgc agggtgaaaa accggtaatg cttcaggaca tcataagccg ttgcggcaac
    23761 catcatcggc acagccaaga aaaacgagaa ttctgtcgca gttttccgtt cgattcccca
    23821 aagcatcccg cccataatcg tactgcccga acgggacgta cccggaatca gtgcaaacac
    23881 ttgggcaacg ccgatcatca aggcatcaat cggacgcaat gcatcaacat cggcaatttt
    23941 aggctctgct cggctttggc gtttctccac ccacaaaata aaaaaaccgc ccaaaaccag
    24001 catgactgca acactcaagg ggttaaacag atactctttg atttgtttgc cgaacaacag
    24061 ccccatcacg gcggcaggta taaaagcaat ggcaagattg aggacgaagc ggttggcttt
    24121 ccggtctttt cccacgccat gcaacacatt gctgaaacgt tgccggtatt caaacactac
    24181 cgccaaaacc gcaccgagct ggatggcaat ttcaaaaacc ttgtgattgc tgtgaaaacc
    24241 aatcagattg ccgaacacaa tcaaatgccc ggtgctggaa atcggtaaaa attcggtaaa
    24301 accttctacc aagcccatca tcagggcttt caggacaatc agaaaatcca ttgcttgcgc
    24361 ttctttcgga tacgggagtt cggctatttc tgtacagcag gggtctgacg cttgcgttct
    24421 tccctgactt tggcaaccaa ttctttaatc gtatgaacgc cgccgtcaaa gccgttattg
    24481 aagataacgc ggtattttcc gccgacaata acggtcggcg tgctgtcaat accgtattgt
    24541 tccgtcagtt tctgcatttt taatgcggcg gcggcagctt cgggagaatc ataggcgcgc
    24601 atcagttttt tgccgtcaaa gcctttttga gacaaagccc actttccggc aaccgacctg
    24661 ttttccaagc ggattttttg ttcgtaaact gctttaaaca cagcagggtt tgcctgatat
    24721 ttcaaacccg acaaattgac ggcagccgcc atcctggcca gaccgagcat ttcaggctgc
    24781 cagaccacgt gctccgtcct caaataggca tcagacggca atgccttgcc caatttcaat
    24841 aacaaaggat cgaaatgatg gcaatgtacg cagaaatagc cgaaaaattc caaaacctca
    24901 attttgcccg actgttcttg aggaatgggt ttatccaaca caagatagtc ttccctttcc
    24961 gtcagggcat atgcctgcgc ggacaacact gccgacagca gcagcggcaa cagatgtttg
    25021 agcttcataa ttatttgctt tcgatagaac ggatcaggct ggcgacttca tgttttttca
    25081 actcgtcctg catttttttc accgcatcgg cagacatatt gccgctttgc acccggtaaa
    25141 gcgttttatg tcccgcctga taaccgacca ccttggaaga tatgcccaag attgccagtt
    25201 tggcacgctg tccttccgcg ctccggcggt cggcatacgc gcccatttgc agataatgcg
    25261 ttgcttccgc cttgtcgggc gttttcattt tctgcacttc tttggcagcg gcactgcgcg
    25321 ctttttcgat gctgccgctg ttgaggattt gttctggggt cggtttgggt gcaacttttt
    25381 ccttttccgc ctttttctct tcttttgaag cttttttctc tgtttcttta gacggtttta
    25441 ccgcttgttt tttaaccgtt tcggcatctt tcttctgcgc tttttccccg acggtttgtt
    25501 cacgctcttc cgtcagtgct ttcttacgca ctgcctgccc gtccgacttt tcccgctccg
    25561 actcgcccgc cttttcctca acctcgtcgg ctttgtcggc aatgggctgc ttgtcggcag
    25621 ctttttccgc atccgactgc tctgcctctt tcgtagcatc cggttcggac aaggcgtttt
    25681 gatcggccgg ttcaggttgg atgtcttcct taggctggtt tttcggtttc aggatttccg
    25741 tttctgcagg ctgcttcgac gaagccggga ttttgaacgc attttgaccg ctctggttca
    25801 gataaaacaa aataccggca ataatgaccg tcgccagtat caaaccgaag aaaaaaccgg
    25861 acaaaccttt tccggattgg gaaaatttgt tcataaacat accttaatgt gtttcagacg
    25921 gcattaacgc cgtttgctta cgggcggata ttctaacaat atcgccatat ttgggcaaaa
    25981 cctgcttcca ttcccgttcc tataaagcgc ggcggaaacc tatggctttc atgacgtggc
    26041 ttctgccgac ttcttcgtcg cctgccaaat ccgccaatgt acgcgccacg cgcataatgc
    26101 ggtggaagct gcgggcggag agggagagtt tttccaacag gttgcccaag gcttcctgcg
    26161 cttctttttg gatgcgggct tgggaatcga gttcgctgac gctcaaagcg gcgttgactt
    26221 tgccctgccg tgcgtattgt ttgtcgcggg cggcgataac gcgttccaaa acggacgcgc
    26281 tgctttcccc tgcttcctgc tgcatcagtt cggcggcgga caggctggga acttcgatgg
    26341 tcaaatcgat gcggtcaagc agcgggccgg aaatcttgct gcggtaacgc gcgacgcttt
    26401 cgggcgtgca acggcagggt ttgacgggat gcccgagata accgcacggg caggggttca
    26461 tggcggcgac aagctggaat ttggcgggat agacggcttg gcgcgccgcg cgggaaatgt
    26521 ggatttcgcc gttttccaaa ggttcgcgca acacttccaa aactttgcgg tcgaactcgg
    26581 gcaattcgtc caaaaacaaa acgccgtggt gcgccaatga aatctcgccc ggacgcggat
    26641 ccgagccgcc gccgaccata gccgccgcgc tggcgctgtg atgcggactg cggaaaggac
    26701 ggttgctgtc gagttgttgc tggtggttgg gcaggagcga acgcaatgcc caaacttcaa
    26761 ccaattcgtc ttcggtcagc ggcggcagga tgcctggcag ccgttgggag agcatagact
    26821 tgcccgttcc tggcggaccc atcatcaaga ggctgtgtcc tcctgcggca gcgatttcca
    26881 aagcaaggcg cgcggtgtgc tgacctttca catcgcacaa atcaggttgt ccgccatgtt
    26941 caaacggcat ctgaggaact tggcattcgg tttgcgccaa aggttcgatg ccgttcaaat
    27001 gggcggcgac ttcgcccaaa gagcgcgcgc cgtaaacggt aatgccgcgc atcacggcgg
    27061 cttgtcctgc attttcttca ggcaaaacaa atgcacgttt tgcctgcata ccctgccacg
    27121 ccatcgccaa cgcgccgcgc acggggcgca acagccccga cagtgccaat tcccccgcaa
    27181 actcgtattc cgccagtttt tcgggcgcaa cctgccccga tgcggcaagg atgccgattg
    27241 caatcggcaa atcgaaacgc cccgactctt tgggcaggtc ggcgggggcg aggttgacgg
    27301 taattttttt ggcggggaat tcaaaaccgc tttgaataat ggcggcacgg acgcggtcgc
    27361 gactttcctt tacttccgta tcgggcagcc cgacgatgtt gaaatgcggc aggccgttgg
    27421 caaggtgggc ttccacttcg accaacggcg cattcatacc gctcaaggcg cggctgtaaa
    27481 ccaaggcaag cgacatattt cagacggcct tattcgccgg cttcggtttg ctgcctgatt
    27541 tcggcgacgg cttcttcggc agcggcttca gccgcttcca atgctgcccg ttcgggattt
    27601 tgcgcggctt cgagttttgc caaacgcgct tccaaagccg ccagtttggt acgggttttg
    27661 attaaaacct gctgctggat gtcgaattct tcgcgcgtaa ccagatccat acggttgaac
    27721 gcgccgccca gcatcgcctt aatatttttt tccacatctt tggcagggct gttggcgatg
    27781 gtttcgctga ttttcgagcc gacttcctca aaaagctgct tgccgaacat aatctgtatc
    27841 cttcctgaac atatcaaatt caatcggcta ttgtataagg aaaaatgccg tctgaaaacg
    27901 ggcggcggat aatcggcaaa acataccgcg cctcctttgc ggttgctaaa ttacacctca
    27961 aaacacctgc cgcctgaaaa cggatttcat attccgcgca acgcctgatt acaagacact
    28021 acaaaacaat atgctgtttt aaatgatttt tccgacgcgc atcgtttcaa actcggcttt
    28081 ttaagccatt aagtgctttg caaacaacag aaattgggtt atgctgaaac ggattattta
    28141 caatttcata tagttttatt acatatctta ttgtgattga agataattta tccgaatccc
    28201 cctttcgggt atccggattt tccgttgtac ttttattaga aaaacatttc aggcgcaagt
    28261 tgcttgcaat ttcaaagcct gtaattctga agttttagac gaggaatagc acatgaatcc
    28321 catgtacatt acttttgcaa tctatctggt tgcagtcctt ctcatcgggc ttgccgccta
    28381 tttttctacg cgcaatttcg acgattatat tttgggcggg cgcagcctag gcccgtttgt
    28441 taccgcgatg tcggcaggcg cgtccgatat gtccggctgg ctgctgatgg gtctgccggg
    28501 cgcgatttac ctgagcggtt tgaatgaggc ttggattgcc atcggcctct tggtcggcgc
    28561 gtatttcaac tggctcttgg tggcgggccg cctgcgcgtg cataccgaat acgccaacaa
    28621 tgcgctgacg ctgccggatt atttcttcca ccgcttcggc gcgggcggac acttgatgaa
    28681 agtggtttcc gcactgatta tcctgttctt cttcacgatt tattgcgcct cgggcattgt
    28741 ggcgggcgca accctgttcc aaagcctgtt tgaaggtatg acttacaatc aggcaatgtg
    28801 gctgggcgcg ggcgcgacca tcgcctatac cttcttgggc ggtttcttgg cggtcagctg
    28861 gacggatacg ctgcaggctt ctttgatgat tttcgccctg attttaacgc cggtgatggt
    28921 ctatctgggc ttgggcggcg cggaacagat gtctgccgcg attcaaagcg tcgccgcagg
    28981 cacgggcaaa gaatacggca gcctgtttgc cggtacgacc gtcatcggca tcatttccac
    29041 cgccgcatgg ggcttgggct atttcggaca gccgcacatt ttggcgcgct ttatggcggc
    29101 cgaaagcgcg aaatcgctgg tatccgcacg ccgcatcggc atgacttgga tggcgttgtg
    29161 ccttgcgggc gcggtagcgg tcggttattt cggcattgcg tatttcggtg caaatcccga
    29221 caaagtttct tctatgagcg gcaaccacga acgcatcttc atcgcgcttt ccaccctgct
    29281 gttcaaccct tggatcgccg gcattatttt gagcgcgatt ttggcggcgg tgatgtccac
    29341 cctgtcctgc cagcttttgg tttgctccag cgcgattacc gaagacttct acaaaggctt
    29401 tttgcgtaaa aacgcgcaac agtcggaact ggtatgggtc ggccgcctga tggtcttggc
    29461 gattgccgtg atttccatcc tgattgcttc cgatccgaac agcaaagtat tgggactggt
    29521 gtcttacgcg tgggcaggtt tcggtgcggc attcggcccg attgtgattc tgtccgtatt
    29581 gtggaaacgc atcactgcct acggcgcgtt gtcgggtatg gttgcaggtg ccttaaccgt
    29641 ggtcgtatgg gcggaatggg tgaaaaaacc ggctcaagcg gcaggcgaaa gcggcttgtt
    29701 gacgatgtac gaaatcgtcc cgggctttat tgtttgcttg atcgttgccg tgttggtttc
    29761 cctgttcaac aaagaacctt cgcgcgaaac ccaagaacgc tttgaaaaag ccgacgcaga
    29821 ttaccgcgcc gcccgataaa cccctttcag acggcacggc atcccggttc gtgccgtctg
    29881 aacccgtttc cgggtcaaac acaaataaac cttgcctgca tacaggcctt atttgtgttt
    29941 tacccgtttc attttcaaag aaacggaagc acctaccctg cccgcatttg aagccttgta
    30001 tttcatctgc ggatttatta cgatattacc gtattcagac cgcaccgatg ccgtctgaac
    30061 cccccgaaaa actttggaga atccaaaaaa tgtttcattt tgcatttccg gcacaaactg
    30121 ccctgcgcca agcgataacc gatgcctacc gccgtaatga aatcgaagcc gtacaggata
    30181 tgttgcaacg tgcacagatg agcgacgaag agcgcaacgc cgcctccgag cttgcccgcc
    30241 gtttggttac ccaagtccgc gccggccgca ccaaagccgg cggcgtggat gcgctgatgc
    30301 acgagttttc actctccagc gaagaaggca tcgcgctgat gtgtctggca gaagccctgc
    30361 tgcgtatccc cgacaacgcc acgcgcgacc gcctgattgc cgacaagatt tcagacggca
    30421 actggaaaag ccatttgaac aacagccctt ccctcttcgt caacgccgcc gcctggggcc
    30481 tgctgattac cggcaaactg accgccacaa acgacagaca aatgagttcc gcactcagcc
    30541 gcctgatcag caaaggcggc gcaccgctca tccgccaagg cgtaaattac gccatgcgcc
    30601 tgttgggcaa acagttcgta accggacaga ccattgaaga agccctgcaa aacggcaaag
    30661 aacgcgaaaa aatgggctac cgcttctcct tcgatatgtt gggcgaagcc gcctacaccc
    30721 aagccgatgc cgaccgctac taccgcgact atgtcgaagc catccacgcc atcggcaaag
    30781 atgcggcagg acaaggcgtt tacgaaggca acggcatttc cgtcaaactt tccgccatcc
    30841 atccgcgcta ctcgcgcgcc caacacggcc gtgtgatggg cgaactgttg ccgcgcctga
    30901 aagagctgtt ccttttgggt aaaaaatacg atatcggtat caacatcgat gccgaagaag
    30961 ccaaccgtct ggagctgtct ttggatttga tggaggcttt ggtttcagac cctgacttgg
    31021 ctggctacaa aggtatcggt ttcgttgtcc aagcctacca aaaacgttgt ccgttcgtta
    31081 tcgactacct gatcgacctt gcccgccgca acaaccaaaa actaatgatc cgcctcgtca
    31141 aaggcgcgta ttgggacagc gaaatcaaat gggcgcaagt ggacggcttg aacggctatc
    31201 cgacctacac ccgcaaagtc cacaccgaca tctcctacct cgcctgcgcg cgcaaactgc
    31261 tttccgcgca agacgcggta tttccgcaat tcgccaccca caacgcctac accttgggcg
    31321 caatctacca aatgggcaaa ggcaaagact ttgaacacca atgtctgcac ggcatgggcg
    31381 aaaccctgta cgaccaagtc gtcggcccgc aaaacttagg ccgccgcgtg cgcgtgtacg
    31441 ccccggtcgg cacacacgaa accctgctcg cctacttggt gcgccgcctg ttggaaaacg
    31501 gcgcgaactc gtctttcgtc aaccaaatcg tcgatgaaaa catcagcatc gacacgctca
    31561 tccgcagccc gttcgacacc atcgccgaac aaggcatcca cctgcacaac gccctgccgc
    31621 tgccgcgcga tttgtacggc aaatgccgtc tgaactcgca aggcgtggac ttgagcaacg
    31681 aaaacgtatt gcagcagctt caagaacaga tgaacaaagc cgccgcgcaa gacttccacg
    31741 ccgcatccat cgtcaacggc aaagcccgcg atgtcggcga agcgcaaccg attaaaaatc
    31801 ctgccgacca cgacgacatc gtcggcacag tcagctttgc cgatgccgcg cttgcccaag
    31861 aagcggttgg cgcagccgtt gccgcgttcc tcgaatggag tgcgacacct accgccgaac
    31921 gcgccgcctg cctgcgccgt ttcgccgacc tgctcgaaca gcacaccccc gcgctgatga
    31981 tgcttgccgt gcgcgaagcg ggcaaaacgc tgaacaacgc cattgccgaa gtgcgcgaag
    32041 ccgtcgattt ctgccgctac tacgcaaacg aagccgaaca caccctgcct caagacgcaa
    32101 aagccgtcgg cgcgattgtc gccatcagcc cgtggaactt cccgctcgcc atctttaccg
    32161 gtgaagtcgt ttccacattg gcggcaggca acaccgttat cgccaaacct gccgaacaaa
    32221 ccagcctgat tgccggttat gccgtttccc tcatgcacga agccggcatc ccgacttccg
    32281 ccctgcaact cgtcctcggc gcaggcgacg tgggtgcggc attgaccaac gatgcccgca
    32341 tcggcggcgt gattttcacc ggctcgaccg aagtggcgca cctgatcaac aaagcccttg
    32401 ccaaacgcaa cgacaatccc gtcctgattg ccgaaaccgg cggacaaaac gccatgattg
    32461 tcgattccac cgcacttgcc gagcaagtct gcgccgacgt attgaactcc gccttcgaca
    32521 gcgcgggaca acgctgctcc gccctgcgca ttttgtgcgt ccaagaagac gttgccgacc
    32581 gtatgctcga catgatcaaa ggcgcgatgg acgaactcgt cgtcggcaaa ccgattcagc
    32641 tcactaccga tgtcggcccc gtcatcgatg ccgaagcaca gcaaaacctg ttgaaccaca
    32701 tcaacaaaat gaaaggcgtt gccaagtcct accacgaagt caaaaccgcc gccgatgtcg
    32761 attccaaaaa atccacgttc gttcgcccca tcctgtttga attgaacaac ctcaacgaac
    32821 tgcaacgcga agtcttcggc cccgtcctgc acgtcgtccg ctaccgcgcc gacgaactcg
    32881 acaacgtcat cgaccaaatc aacagcaaag gctacgccct gacccacggc gtacacagcc
    32941 gcatcgaagg cacggtacgc cacatccgca gccgcatcga agccggcaac gtttacgtca
    33001 accgcaacat cgtcggcgca gtcgtcggcg tacagccctt cggcggacac ggtctgtccg
    33061 gcacaggccc caaagcaggc ggttcgttct acctgcaaaa actgacccgc atccccgaat
    33121 gggttgcccc gaccctgagc caaatcggac aggcggacga agccgcactc aaacgcctcg
    33181 aagcactggt tcacaaacta ccgttcaacg ccgaagagaa aaaagccgca gcggccgctt
    33241 tgggacacgc ccgcatccgc accctgcgcc gtgccgaaac cgtccttacc ggcccgaccg
    33301 gcgagcgcaa cagcatctca tggcacgcgc caaaacgcgt ttggatacac ggcggcagca
    33361 cggttcaagc ctttgccgca ctgaccgaac ttgccgcctc cggcgtacag gcagtggtcg
    33421 aacctgacag cccctgggct tcctacaccg ccgacttgga aggtctgctg ctggtcaacg
    33481 gcaaacccga aaccgccgac atcagccacg ttgccgccct gtcgcctttg gacagcgcgc
    33541 gcaaacagga acttgccgcc cacgacggcg cactcatccg catcctccct tcggaaaacg
    33601 gactcgacat cctgcaagtg tttgaagaaa tctcttgcag cgtcaacacc acagccgccg
    33661 gcggcaacgc cagcctgatg gcggttgccg actgattttg ccgaaatccc cgggcgcggc
    33721 ccgtgaacca atgccgtctg aaaacctttc agacggcatt tttata
//
LOCUS       DAWFJR010000014        33705 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWFJR010000014 DAWFJR010000000
VERSION     DAWFJR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33705
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.014"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(82..456)
                     /locus_tag="WGR04_000570"
     CDS             complement(82..456)
                     /locus_tag="WGR04_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0794182.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGVNKSTAAYYFHR
                     LRLLIYQNSPHLEMFDGEVEADESYFAERQNHINGIGNFWNRAKRHLRKFNGIPKAHF
                     ELYLKGYERRFNNSEIKVLVPF"
     gene            complement(762..2021)
                     /gene="rho"
                     /locus_tag="WGR04_000571"
     CDS             complement(762..2021)
                     /gene="rho"
                     /locus_tag="WGR04_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ0794183.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTQQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEAAEFLIGKIKASKNNDDFFELMRGK"
     gene            complement(2240..4624)
                     /gene="ppsA"
                     /locus_tag="WGR04_000572"
     CDS             complement(2240..4624)
                     /gene="ppsA"
                     /locus_tag="WGR04_000572"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ0794184.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISVALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFSINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVAESFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            5035..5856
                     /locus_tag="WGR04_000573"
     CDS             5035..5856
                     /locus_tag="WGR04_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ0794185.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(6095..6757)
                     /locus_tag="WGR04_000574"
     CDS             complement(6095..6757)
                     /locus_tag="WGR04_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0794186.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLIKLGAGITPDHTDYTRWRAEYLDEYDSRYAQDTTLFGGVNELIAELGKRG
                     IKWGIITNKPMRFTDKLVPKLGFIVPPAVVVSGDTCGEPKPSVKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QS"
     gene            complement(6796..7623)
                     /locus_tag="WGR04_000575"
     CDS             complement(6796..7623)
                     /locus_tag="WGR04_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ0794187.1"
                     /translation="MKPDIYALLERALLSGNPDEKGRLADEAFAAVQNADGAETNAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTTEGYAAMLHAITHIEFNAVNLTLDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPIALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKHAV"
     gene            7901..8524
                     /gene="lolA"
                     /locus_tag="WGR04_000576"
     CDS             7901..8524
                     /gene="lolA"
                     /locus_tag="WGR04_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ0794188.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            8797..9936
                     /locus_tag="WGR04_000577"
     CDS             8797..9936
                     /locus_tag="WGR04_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0794189.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQAPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            10069..11111
                     /locus_tag="WGR04_000578"
                     /pseudo
     CDS             10069..11111
                     /locus_tag="WGR04_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            11152..11682
                     /locus_tag="WGR04_000579"
     CDS             11152..11682
                     /locus_tag="WGR04_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ0794190.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLEGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(11716..11832)
                     /locus_tag="WGR04_000580"
     CDS             complement(11716..11832)
                     /locus_tag="WGR04_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HEZ0794191.1"
                     /translation="MPSKPEKRASDGIFACNSNADGQNQGASDTVIGCIPSA"
     gene            complement(11851..12039)
                     /locus_tag="WGR04_000581"
     CDS             complement(11851..12039)
                     /locus_tag="WGR04_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor"
                     /protein_id="HEZ0794192.1"
                     /translation="MPSEPEKRLSDGIFACNSNADGQNQDASDTVIGHPVLMNLGLTQ
                     ERWPDVVFHETREHSVKL"
     gene            complement(12058..13653)
                     /locus_tag="WGR04_000582"
     CDS             complement(12058..13653)
                     /locus_tag="WGR04_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ0794193.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LKIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIENPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIEWAPA
                     PKPRDATVRMVEPDEAKFSGFIFKIQANMDPKHRDRIAFLRVCSGRFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANVGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(13760..14155)
                     /gene="hisI"
                     /locus_tag="WGR04_000583"
     CDS             complement(13760..14155)
                     /gene="hisI"
                     /locus_tag="WGR04_000583"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ0794194.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(14186..14953)
                     /gene="hisF"
                     /locus_tag="WGR04_000584"
     CDS             complement(14186..14953)
                     /gene="hisF"
                     /locus_tag="WGR04_000584"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ0794195.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNSENTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(14966..15703)
                     /gene="hisA"
                     /locus_tag="WGR04_000585"
     CDS             complement(14966..15703)
                     /gene="hisA"
                     /locus_tag="WGR04_000585"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ0794196.1"
                     /translation="MLLIPAIDLKEGHCVRLRQGLMEEATVFSDSPAETALHWFEQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(15736..16374)
                     /gene="hisH"
                     /locus_tag="WGR04_000586"
     CDS             complement(15736..16374)
                     /gene="hisH"
                     /locus_tag="WGR04_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ0794197.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPDRVSR
                     ADKVIFPGQGAMPDCMAALTRDGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFARDLRDRQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(16505..18007)
                     /gene="pta"
                     /locus_tag="WGR04_000587"
     CDS             complement(16505..18007)
                     /gene="pta"
                     /locus_tag="WGR04_000587"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ0794198.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRVNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDIVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(18192..19250)
                     /gene="fbpC"
                     /locus_tag="WGR04_000588"
     CDS             complement(18192..19250)
                     /gene="fbpC"
                     /locus_tag="WGR04_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ0794199.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(19271..20788)
                     /gene="fbpB"
                     /locus_tag="WGR04_000589"
     CDS             complement(19271..20788)
                     /gene="fbpB"
                     /locus_tag="WGR04_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ0794200.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(20856..21851)
                     /gene="fbpA"
                     /locus_tag="WGR04_000590"
     CDS             complement(20856..21851)
                     /gene="fbpA"
                     /locus_tag="WGR04_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ0794201.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALITYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(22184..22567)
                     /locus_tag="WGR04_000591"
     CDS             complement(22184..22567)
                     /locus_tag="WGR04_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEZ0794202.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHNGKLESMDGREQELVLIW
                     RAEADIGNGGILQFVCNWCFPAAEKTSSVLKKIGAIHSAMLIHRAADALDKEIRRLQS
                     EGKNLKEMWDITSRQQNRLTAEQSG"
     gene            complement(22625..24001)
                     /gene="argH"
                     /locus_tag="WGR04_000592"
     CDS             complement(22625..24001)
                     /gene="argH"
                     /locus_tag="WGR04_000592"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ0794203.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIRSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(24020..24889)
                     /gene="galU"
                     /locus_tag="WGR04_000593"
     CDS             complement(24020..24889)
                     /gene="galU"
                     /locus_tag="WGR04_000593"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ0794204.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(24917..25516)
                     /gene="rdgB"
                     /locus_tag="WGR04_000594"
     CDS             complement(24917..25516)
                     /gene="rdgB"
                     /locus_tag="WGR04_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ0794205.1"
                     /translation="MSEKPEKIVLASGNAGKLEEFGNLFKPYSITVLPQSAFGIPECP
                     EPYPTFVENALAKARHAAEYSGLPALADDSGICAAALNGAPGIHSARYAGDNPKSDTA
                     NNLKLAAELAGKTDKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALAELLRKLAL"
     gene            complement(25509..25742)
                     /locus_tag="WGR04_000595"
     CDS             complement(25509..25742)
                     /locus_tag="WGR04_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794206.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGSLALYLRHKSLRAAQSAAKENRYV"
     gene            complement(25850..26383)
                     /locus_tag="WGR04_000596"
     CDS             complement(25850..26383)
                     /locus_tag="WGR04_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ0794207.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(26485..26943)
                     /gene="nudB"
                     /locus_tag="WGR04_000597"
     CDS             complement(26485..26943)
                     /gene="nudB"
                     /locus_tag="WGR04_000597"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ0794208.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPEGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            27154..27230
                     /locus_tag="WGR04_000598"
     tRNA            27154..27230
                     /locus_tag="WGR04_000598"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27188..27190,aa:Pro,seq:ggg)
     gene            27389..29299
                     /locus_tag="WGR04_000599"
     CDS             27389..29299
                     /locus_tag="WGR04_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223961.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HEZ0794209.1"
                     /translation="MGISRIISLILSILAVCLPMHAHASDLANDPFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGKIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNINSNFSQHAQLEREIKKLRTSEGK
                     ANSASAALLTLDLKTAQAANQVVESLRSTGNLPSNYITKSQAISMGWRPGKALGNTIK
                     NGQLGGDIFANSTNILPAKQGRIWYEADIGLINTMSRSNQAGTRLLYSNDGLLYITTD
                     HYISAIQIGTWK"
     gene            29311..29598
                     /locus_tag="WGR04_000600"
     CDS             29311..29598
                     /locus_tag="WGR04_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEZ0794210.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNQLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
     gene            30094..31350
                     /locus_tag="WGR04_000601"
     CDS             30094..31350
                     /locus_tag="WGR04_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ0794211.1"
                     /translation="MKSKSGVIASLTGAAVGGTPVDAQTGGAVGQNAVENNLYLTSEA
                     LKKDKQTARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDSRFKDLKKE
                     SLYYLNKHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGS
                     CAETFGLGCAAALVGVTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEY
                     VQFSIDLFSVGKSGGGIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQS
                     SQFKAHTQREWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGV
                     YQATVEIKKPDGSWEVKTKKGGKVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNK
                     WQGTSKSGIKIEGFTEPNRTAYPIYE"
     gene            31381..31800
                     /locus_tag="WGR04_000602"
     CDS             31381..31800
                     /locus_tag="WGR04_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794212.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            31837..32811
                     /gene="mafB"
                     /locus_tag="WGR04_000603"
                     /pseudo
     CDS             31837..32811
                     /gene="mafB"
                     /locus_tag="WGR04_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
     gene            32822..33283
                     /locus_tag="WGR04_000604"
     CDS             32822..33283
                     /locus_tag="WGR04_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794213.1"
                     /translation="MNLETAENIQCEFEYLALDGYFPMHFASHGQGNKDWQFAVEFIY
                     RLLICQLATLEPINFETKNDILDFCHNLAKQSPFNNDNEVWYQGEIVLTKKGIDLIKE
                     YIPEAFEEWNGKKFELNIPFIKTLKNIFVNYEVAWDENNPLFPIISLNLGT"
     gene            33309..33557
                     /locus_tag="WGR04_000605"
     CDS             33309..33557
                     /locus_tag="WGR04_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ0794214.1"
                     /translation="MEIKKPDGSWEMKKGRGGKPMTNHSMFPKDWDEARIRAEVTSAW
                     EKRKELEGQKWRGTSKSGVEIEGFTEPNRTAYPLYNSK"
BASE COUNT         7972 a         7921 c         9510 g         8302 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataaaaa tttatggggc tgtcctagat aactaggata
       61 aactcgattt tactaattgt tttaaaatgg aacaagaact tttatctcac tgttgttaaa
      121 acgccgttcg taccccttta aatacagctc aaaatgcgct ttgggaatgc cgttaaactt
      181 gcgtaaatga cgttttgccc ggttccaaaa gttcccaatt ccattgatat ggttttgtcg
      241 ttcagcaaaa taactttcat ctgcttctac ttcgccgtca aacatttcca aatgcggact
      301 gttttgataa ataagtaatc gtaaacgatg aaaataatag gctgcggtac ttttattaac
      361 gcctactaac tctgctgctg ttcttgcagt tacacctgcg acaaataact caatgagttt
      421 attttgttta taccggctta gacgactttt tctcataggg gcaactctaa cttaatttga
      481 atttccctag ttatctagga cagccccaaa tttatacaaa aatgagcgcg gttcggcgca
      541 accttgaatc aagttcccgc atcggttttc attgccggta cggatgcgtt caagccggct
      601 ttgcaaaggc cgcgctttcg gcaagcggac acggacactg ccggcggttg cgccgttaac
      661 ggggggaggg aggagctgcg ccggccgtgt gaatgaaagt gccgtctgaa acccgatttt
      721 caggcttcag acggcatttc gcattaatgc gggtggcgcg tttatttgcc gcgcatcagt
      781 tcaaagaaat catcattgtt tttagaggct ttgattttcc cgattaaaaa ttcggctgcc
      841 tcgatttcgt ccatcgggtg caggaacttg cgtaagagcc acatacgttg caactggtcg
      901 ttcggcacaa gcagctcttc gcggcgcgtg ccggatttgt tgatgttgat ggcggggaag
      961 aggcgttttt ccgccatacg gcggtcgagg tgcaattcca tattgccggt gcctttgaat
     1021 tcttcgtaaa tcacatcgtc catacggctg ccggtttcaa ccaatgctgt ggcaatgatg
     1081 gtcagcgaac cgccttcttc cacgttgcgc gccgcgccga agaaacgttt gggacggtgc
     1141 agtgcgttgg catcgacacc gccggtcagg attttgcccg aggtaggcac gacggtattg
     1201 taggcgcggg caaggcgggt aatcgaatcc agcaggatga ccacgtcttt tttgtgttcc
     1261 accatacgct tggctttttc aagtaccatt tcggcaactt ggacgtggcg ttgcgccggc
     1321 tcgtcaaatg ttgaagaaac cacttcacct cggacggagc ggctcatttc ggtaacttct
     1381 tcgggacgct cgtcaatcaa gaggacgatg agctcgactt caggatagtt tgcggtaacg
     1441 gcgtgggcaa tattttgcaa catcacggtt ttaccgcttt tgggcggggc aaccaagagg
     1501 gcgcgctgac ctttgccgat gggggaaatc agatcgatgg cgcgtccggt caggttttct
     1561 tcggacttta agtcgcgttc cagcttcaac tgttgggtcg gaaacagcgg ggtcaggttt
     1621 tcaaacagga ttttatggcg gcatacttcc gggtggtcgc cgttgatggt atcaagcctg
     1681 accagggcaa aatagcgttc gttgtctttg gggacgcgca cgctgccttc gatggtgtcg
     1741 cccgtatgca ggttgaagcg gcggatttgg gtgggcgaga catagatgtc gtcggggccc
     1801 gcaagatagg acgtgtccgc gctgcggagg aagccgaagc cgtcgggcag gatttcgagc
     1861 gtgccggagc aggtgaaacc ctcgcctttt ttcatcattt ggcggacgat ggcaaatacg
     1921 aggtcttgtt tgcggaatcg gttggcgttt tcgatgccgt gttcttccgc caattctaag
     1981 agtttggaaa tgtgcagggt ttgtaattcg gagacgtgca taataatgat gtattttgaa
     2041 gaggaaaaag acaggcagat gccgtctgaa agaagaagct gaccgttggc ggttgctcgg
     2101 agaaggggga attgtaggca gtcggcgcgt gggtgtcaaa tattatcgcg gacggggcat
     2161 cggcaggaaa tgccgtctga gcggagctgc ttggaaaaaa tacccccgcg cttttcaggc
     2221 tcgggggtat gggcattgat tatttgttca attcattcgc caaatacagc caagtttcga
     2281 tgacggtatc cgggttcagg gaaacgcttt caatgccttc ctcaaccagc catttggcaa
     2341 agtccggatg gtctgacggg ccttgaccgc agatgccgac atatttgttc tgcttgcggc
     2401 aggcggagat ggcaagatgc agcatcactt tgacggcagg gttgcgttcg tcaaacgatt
     2461 cggataccaa gccgctgtca cggtcgaggc cgagggtcag ctgggtcatg tcgttagagc
     2521 cgatggagaa gccgtcgaag tattgcagga attgttctgc caatacagcg ttgcttggca
     2581 actcgcacat cataatcagg cgcaggccgt ttttgccgcg ttccaaaccg ttttctttca
     2641 gggctttaac aacggcttcg gcttcgccca aagtgcggac gaacggaatc atgatttcga
     2701 cgttggtcag acccatttcg tcacggacgc gtttcaaggc tttgcattcg agggcgaagc
     2761 agtctttgaa actttcggcg acataacgcg ccgcaccccg gaagcccaac atcgggtttt
     2821 cttcgtgcgg ttcgtatacg ttgccgccga ccaggttggc gtattcgttg gatttgaagt
     2881 cggacatacg gacgatggtt ttacgcggat aaaccgatgc ggccaatgtc gccacgcctt
     2941 cggcgatttt atcgacgtag aagtcgacag gggacgcgta accggcgata cggcgggtaa
     3001 tttccgcttt taattcgtcg tcttgtttgt caaattccaa caaggctttg gggtggatac
     3061 cgatttggcg gttgatgata aattccatac gcgccaagcc gatgccttcg ctgggcaggt
     3121 tggcgaagct gaatgcgagt tcgggattgc cgacgttcat catgactttt acaggtgctt
     3181 taggcatgtt gtccaaagca acatcggtaa tttgtacgtc cagcaggccg gcatagatga
     3241 agccggtatc gccttcggca caggatacgg taacttcttg accgtttttc agcaattcgg
     3301 ttgcattgcc gcagccgacg acggcaggaa tgcccaattc acgcgcgata atcgctgcgt
     3361 ggcaggtgcg tccgccgcga ttggtaacga tggcggaagc gcgtttcatc accggttccc
     3421 aatccggatc ggtcatatcg gtaaccagta cgtcgccggc ttcgacggaa tccatctcgg
     3481 aagcatcttt aatcaggcgt actttgccct gtccgacttt ctgaccgatg gcgcggcctt
     3541 cgcacaagac ggttttttcg ccgttgatag aaaagcggcg caggttgcgg ctgccttctt
     3601 cttgggattt cacggtttcg ggacgggctt gcaggatata gagtttgccg tccaagccgt
     3661 cgcgacccca ttcgatatcc atcgggcggc cgtagtgttt ttcgatggtc agcgcgtaat
     3721 gcgccaactc ggtgatttct tcgtcggtaa tggagaagcg gttgcggtct tcttcgggga
     3781 catcgacgtt ggttaccgat ttgccggctt cggctttgtc ggtaaaaatc attttgatgt
     3841 gtttcgaacc catggtttta cgcaggatgg cgggcttgcc cgctttgagc gtaggtttga
     3901 acacataaaa ttcgtccggg ttgaccgcgc cttgtacgac gttttcgccc agaccgtaag
     3961 aggaggtaac aaagacgact tgatcgtagc cggattcggt gtcgagggtg aacatcacac
     4021 ctgatgcgcc actgtcggaa cgcaccatgc gttgaacgcc ggcggaaagg gcgacgatgt
     4081 cgtgttcgaa gcctttgtgg acgcggtagg aaatggcgcg gtcgttgtac agggaggcga
     4141 atacatggcg catcgcttct ttaacgtttt ccaagccgtt gatgttcaag aaggtttcct
     4201 gttgtccggc aaatgatgcg tccggcaggt cttcggcggt tgcggaagag cgtacggcaa
     4261 cggaaatgtc cgcaccgccg gcatcggcaa ccattttgtt ccatgccgct tcgatttcgg
     4321 catcgagctg ttcggggaag ggcgtgtcca aaatccattg gcggatttct ttgccgacgc
     4381 gtgccagttc ggcaacgtct tcgacatcca attttgccag tgcgacggaa atgcgttcgc
     4441 tcagaccgtt gtgtgcgagg aatgcgcggt aggcatcggc cgtggtggca aagccgccgg
     4501 ggacgcgaac gcctttttcg gtcagctgac taatcatttc acccagcgag gcgtttttac
     4561 cgcccacgcg ttcaacatct gtcatacgca ggttttcaaa ccagattacg tagttgtcgg
     4621 ccatttgtgt gtccaatcca aaatatgtta aaaaagaaac aaatccgcgt gcttatttta
     4681 agcgattcgt tcctctgctg tcatgtgttt tatccgtttt aaaatcatga tgccgtctga
     4741 aaaattgcgg tttcggcgtg tgtagcggtt tgaaacttgc agccggtata cttctttttt
     4801 tgggtatttt ctttgtaaaa caggtggttt gaataggtta atgttttttc tgtttgattt
     4861 ttttgtttat tttttaaaat tttctgccaa aaaatacttt atataaataa ttttatttca
     4921 aaattatatt gtgtctgttt gggtgtaatc cgaggtaggc gtgctgcggg tgctttcctt
     4981 gtgtctgccg ctgctgttat gatgatattt ttattctgta tttaaggatg gaagatgagc
     5041 agtccgcgcc atgtgtttta catttccgac cgtaccggtc tgactgccga gaatatcggc
     5101 gaggctttgc tgaaccagtt tggcaatctg tcgttcaaac gccatacgca tccgtttgtc
     5161 gatacgccgg aaaaggcgcg cgcggtggtg gagaaggtca atcggagccg gcaggaaaac
     5221 ggtcagcgtc cgattgcgtt tgtcagtgtg gttgatgacg aaatccgtcg gattatcaaa
     5281 ggggcggatg cttttcagat taatttcttt gagacttttt tgggactgtt ggagaaagaa
     5341 ctcaataccg aagccacggc atccgagcag gggcatcaca gtatcggtaa tacgaagcgt
     5401 tacgatgcgc gtatggaggc ggtgaatttc tcattgaacc acgatgacgg ggtcagcgat
     5461 aagaaccttc aggaagcgga tgtaatcttg atgggtgtat cgcgttcggg caaaacgccg
     5521 acctgccttt acctcgccct gcaatacggc atccgtgcgg caaactatcc gctgattccc
     5581 gacgatttgg aatcggccga tctgccgcgt atggtcaagc cttataggga taagctgttc
     5641 gggttgacca tccagccgga acgtttgcag gccatccgcc aagagcgccg cccgaattca
     5701 acttatgcca aaatcgatac atgccgcagc gaggtggcgg acgcgcagag tatgttcaga
     5761 cggcatggca ttccgtttgc gaatacgacg gataaatcag tggaagagct tgccgtacac
     5821 atccttcagg cgtgcaagct caaacgcagg ttttgacggg ctttgattcg gtttgaaggc
     5881 ggaaatgccg tctgaaatca ggtttcagac ggcagtttta tagtggatta acaaaaatca
     5941 ggacaaggcg acgaagccgc agacagtaca aatagtacgg agccgattca cttggtgctt
     6001 cagcacctta gagaatcgtt ctctttgagc taaggcgagg caacgccgta ctggtttttg
     6061 ttaatccact atatgtttgt ggggcggata tttttcagga ctgtattttg tccagacatt
     6121 cgagcagatc gagcggcgtg cggatgtgga aatccgcctg ccatgagccg gtatcgtctt
     6181 cgggagcgat gtagccccat tcggcgagga cggttttcat gccggcgttg cggcctgctt
     6241 ggatgtcgcg ttcggcatca ccgacgtaga gtgtgtgttg tgggtcggcg tggatttgtc
     6301 cgcacgcata cagcatgggt ttgacgctgg gcttgggttc gccgcaggtg tcgccgctga
     6361 cgacgacggc gggtgggacg atgaagccga gtttggggac gagtttgtcg gtgaagcgca
     6421 tgggtttgtt ggtgatgatg ccccatttga tgccgcgttt tccgagttcg gcgatgagtt
     6481 cgtttacgcc gccgaagagg gtggtgtctt gggcgtagcg gctgtcgtat tcgtctaggt
     6541 attcggcgcg ccatcgggtg tagtcggtgt ggtcgggggt gatgcctgcg ccgagtttga
     6601 tcagtcctgc cgcgccgtgg ctggcttggg tgcggatttc gtccatgctt tttgcaggta
     6661 gtccgtggcg ggcgagcagg gtgttgagtg cgccgccgag gtctagggcg gtgtcggcaa
     6721 gcgtgccgtc gaggtcgaac aatacggctt gtatcatgtg ttttcctttt ttataaagtg
     6781 cgggactgaa gggtttcaga cggcatgttt attttgtttc aaaccctgtt cgaaatcttc
     6841 caacatatcc aattcgaagc ggctgaagcc cgctttttcc cgcgcttcga tgttcacata
     6901 gccccggaag ataaacatat cgtaacgggc aatcaggctg cggaacaggg caatcggttc
     6961 caaaccgcgt tcgcggcaga ggtgttgata ccaatggttg ccgatggcga catggcccac
     7021 ttcgtcgcgg taaatgatgt ccaatacgcc gcaggtttcc gaatcgccgc gctgcgccac
     7081 cttcgcgcgt atccccggcg taacatccag cccgcgcgct tccaatacgc gcggtaccaa
     7141 cgccatacgc aacagcgggt cgtaggcggt tttgtacgcc atatcccaca aatggttgtg
     7201 cgcctcgaaa ctgccgtaat cgaagccgaa agcgcgcaat ctgtcgcgca tcaggcggaa
     7261 atggtacacc tcttccttcg ccactttcac ccaatcgcgg acaaactgaa acggcagcgt
     7321 gcggaaacgg tatgccgcgt ccaaagttag attgacggcg ttgaactcga tatgcgtaat
     7381 cgcgtgcagc atcgccgcat aaccttcggt tgtgttcatt ttgcgcgggg tcagttgcga
     7441 cggcgcgacc aaaacaggct tgtctggccg tcccgctcgg gggaagtccg ccggcggcgc
     7501 gtttgtttcc gccccgtccg cattttgaac ggcggcaaac gcctcatccg ccagccgtcc
     7561 tttttcatcg gggttgcccg aaagcagggc gcgttccagc aaagcataaa tatcgggttt
     7621 catttcaagt ccgccgtgtt cggaaaacaa atattatagc gtttaaaaaa aacaagatga
     7681 ggcatataat ctccgcgatt cggcattccg cgcccaaacc gtcaaatcac gcggcacaca
     7741 attaaagggc agcgtggcgc gcctaccttt tccgagcggt caaaaaaatc agccctcgga
     7801 aaacgcggtt tgcaaaatgc aaaccgcccg taacgccgcc cgtatgattg ttttgctgcg
     7861 ccgatacttt acgccacacc catcccaaca aggaaaaata atgatgaaac cgcacaacct
     7921 gttccaattt ctcgccgttt gctccctgac cgtcgccgtc gcttccgcac aggcgggcgc
     7981 ggtagacgcg ctcaagcaat tcaacaacga tgccgacggt atcagcggca gcttcaccca
     8041 aaccgtccaa agcaaaaaga aaacccaaac cgcgcacggc acgttcaaaa tcctgcgccc
     8101 gggcctcttc aaatgggaat acacttcgcc ttacaaacag actattgtcg gcgacggtca
     8161 aaccgtttgg ctctacgatg tcgatttggc acaagtgacc aagtcgtccc aagaccaggc
     8221 cataggcggc agccccgccg ccatcctgtc gaacaaaacc gccctcgaaa gcagctacac
     8281 gctgaaagag gacggttcgt ccaacggcat cgattatgtg ctggcaacgc ccaaacgcaa
     8341 caacgccggc taccaataca tccgcatcgg cttcaaaggc ggcaacctcg ccgccatgca
     8401 gcttaaagac agcttcggca accaaacctc catcagtttc ggcggtttga ataccaatcc
     8461 ccaactctcg cgcggcgcgt tcaagtttac cccgcccaaa ggcgtggacg tgttgagcaa
     8521 ctgatgccgt ccgccccgat gccgtctgaa agccgccgag gcttcagacg gcattttcac
     8581 gcaggcggaa caatgtcccg cattaccgcc cgatcgggca tcggaacggc aaacccgtga
     8641 aaattaacgg ttgcgccccg gctgtttttg ccgtttaatg caaaccttgc tgcaccaagg
     8701 gccaagaaag ccgaccggcc gcccccacag cttccgatgc aggcggcccg gccgtccctg
     8761 caatgttttt tatttttgaa cgaaaggtcg aaaaccatga aaaaaacact ggtggcggcg
     8821 gcaatcctga gcctcgcctt gactgcgtgc ggcggcggaa gcgataccgc cgcccaagcc
     8881 ccctccgcca agcccgaagc cgaacaatcg ggcaaactca atatctacaa ctggtcggat
     8941 tatgtcgatc ccgaaaccgt cgccgccttt gaaaaagaaa ccggcatcaa gacgcgttcc
     9001 gattattacg acagcaacga aacactggag gcaaaagtcc tgaccggcaa atccggctat
     9061 gacctgactg cgccgtccat cgccaacgtc ggccggcaaa tcaaagcggg cgcgtatcag
     9121 aaaatcgaca aggcgcaaat cccccattac ggcaacatcg ataaagattt gctgaaaatg
     9181 atggaagccg tcgatccggg caacgaatac gccgtcccct atttctgggg catcaacacc
     9241 ttggcaatca acacgcggca ggtgcaaaag gcattgggta cggacaagct gcccgaaaac
     9301 gaatgggatt tggtgttcaa acccgaatac accgccaaac tcaaatcctg cggcatcagc
     9361 tatttcgaca gtgcaatcga acagattcct ttggcgttgc actatttggg caaagacccc
     9421 aacagtgaga atcccgaaga catcaaagcc gccgtcgata tgatgaaagc cgtccggggc
     9481 gacgtgaaac gcttcagttc ttccggctat atcgatgata tggcggcagg caacctgtgt
     9541 gccgccatcg gttacggcgg cgatttgaac attgccaaaa cccgtgccga agaagccgca
     9601 aacggcgtgg aaatcaaagt attgaccccg aaaaccggcg tgggcgtgtg ggtggattcc
     9661 tttatgattc cgcgcgacgc gcaaaacgtt gccaacgccc accgctatat cgactacacg
     9721 ctccggcccg aggtggcggc gaaaaacggc agcttcgtca cctacgcgcc cgccagccgt
     9781 cccgcgcgcg agctgatgga tgaaaaatac acctccgacg catcgatttt cccgaacaaa
     9841 gaactgatgg aaaaaagttt catcgtatcg cccaaatccg cagaatccgc caaactgggc
     9901 gtgaagctgt ggcaagggct caaagcgggc aaataaccgg aatccctgcc gtctgaaacc
     9961 tttcgggcgg caggaaacgg cgcgtccgtt atcaaacagg ggggcgtttc ccctcctgcc
    10021 ggttatgatt gggttaagat taaaatgatt tagtaaaatg agaaagatat ggatttaagt
    10081 atcgtagttc ccatttataa tgtcgaaagt tatttggaag cgtgtctaaa ttccatagaa
    10141 cctatattaa gtaatgaaaa tgtcgaactt atccttgtga atgacgggtc aaaagacgga
    10201 agtgaagata tatgttataa atatatagat aaaacaacaa acaacaaaca acaaacaaca
    10261 aacaacaaac aacaaacaac aaacaacaaa caacaaacaa caccagatac cgaacaccaa
    10321 acatcaaata tatatatcag gataaccaag ggacggcaga agctaggaat accggaataa
    10381 aaaattcaaa cggaaaatat atagtcttta ttgattcaga tgattttatt aattgtcagg
    10441 tcttgctgga ttttcttagt aaagatgata ttgatatgcc ggatgtggtt tttttaaatg
    10501 cggttaaata tgataaggga agtgtttcac attttggcga agattatcag cctgaaaaaa
    10561 tactcaatca atccaaagtc gaagttttga aaggattatg ccgatttaga aaatttccgg
    10621 gttcggcgtg taataagatt ataaaaaggg aattgattat caaagaaaaa ctgttttttg
    10681 aaaaagaggt ttatgccgaa gatatcgaat ggtcaatgag attatttaat gcggcaacaa
    10741 ctttttctta tttggacggt tgttattact attatcggca gggaagaaaa gattctaaaa
    10801 caggaactgt ttcggaaaaa agtataaagt cattattata tattttggag aaaaatgcgg
    10861 aaatggaatt taatagggat atatcaagtt atctttattc ttttctttcc tacgaatatc
    10921 tcgttttgct ttttatcatg acgagtaaaa atatagagtg tgatgctgat ataaaaagaa
    10981 gggcgtatca tttaaggttt atgctgttaa agtctaataa gttgatatat aagctgatat
    11041 tcccgataat cacattattc ggggttgata ttacaggcag gattttaaaa gcaatcaggg
    11101 ggaatattta ataaatcctt taacactata catcttaccg aaggaggaaa aatgaacgca
    11161 atccgaactt tccaaaaccg cacgcccgaa atccacgaaa gctgtatgat agacgaagcg
    11221 tgcgtcgtca tcggcgaagt gtcgcttgcc gaagacgttt ctgtgtggcc gtgcgccgtg
    11281 ttgcgcggcg atgtgaacag catcaccgtc ggcgcgcgca gcaatataca ggacggcagc
    11341 gtcctgcacg tttcccacaa aaccgccgcc aaacccgaag ggtcgccgct ggttatcggc
    11401 gaagacgtta ccgtggggca caaagtgatg ctgcacggct gccgtatcgg caaccgcgtc
    11461 ctggtcggca tggggacgac ggttctggac gatgccgtga ttgaggacga agtgatgatc
    11521 ggcgcgggca gcctcgtgcc tccgcgcaaa cgtttggagg gcggctatct ttatgtcggt
    11581 tcgccggtca gacaagtgcg cgtgttgacc gatgaggaaa aagccttttt gaaatattcc
    11641 gccgcgcatt atgtgaagct gtcgaaacag tacgggatgt gaaatcacat cggcgttctt
    11701 gcgtcagccc caaattcatg cggacgggat gcatccgata acggtatccg atgcgccttg
    11761 attttgaccg tctgcgtttg agttgcaggc aaaaatgccg tctgaagccc ttttttcggg
    11821 tttagacggc attttatcgc cgattgcttt ttagagtttg accgaatgtt cgcgcgtttc
    11881 gtggaacacg acgtccggcc aacgttcttg cgtcagccct aaattcatga ggacgggatg
    11941 cccgataacg gtatccgatg cgtcttgatt ttgaccgtct gcgtttgagt tgcaggcaaa
    12001 aatgccgtct gaaagccttt tttcgggttc agacggcatt ttattgccga ttgcttttta
    12061 cagtttgacc gaatgttcgc gtgtttcgtg gaacacgatg tccggccagc gttcttgcgt
    12121 gagtcccaga ttcacgcggt tgggggcgag gtaggcgagg ttgccgcccg cgtcgatggc
    12181 gaggttgccc acgttggctt tttcaaattc cgccagtttt ttcttgtcgt cgcacgatac
    12241 ccagcgcgcc gaccagatgg atgcgctgtc aaacacggct tccacgccgt attcgttggc
    12301 cagccgcgag gttacgactt caaactgcaa tacgccgacc gcgcccaaaa tcaaatccgc
    12361 gccgctcatc ggtttgaaca cctgtaccgc gccttcttcg ccgagttgtt gcaggccttt
    12421 ttgcagttgc ttgattttca gcgggttttt gatgcggacg ctgcggaaca gttcgggcgc
    12481 gaagaagggg atgcctgtaa atgccagttg ttcgccttcg gagaagctat cgccgatttg
    12541 gatgttgccg tggttcggga tgccgatgat gtcgccggcg taggcttctt ccaccagctc
    12601 gcggtcgtgc gacatgaatg taaccacgct ggaggcggcg atttcgcggt tgatgcgcag
    12661 gtgtttcatc ttcataccgc gctcgaatct gccggagcag acgcgcaaga aggcgatgcg
    12721 gtcgcggtgt ttcggatcca tattggcttg gattttaaag ataaatccgg aaaacttagc
    12781 ctcgtccggc tcgaccatac gtacggtcgc gtcgcgcggt ttcggcgcgg gcgcccattc
    12841 aatcaatgaa ttgaggattt cctgaatacc gaagttgtta atcgcagagc cgaagaatac
    12901 gggcgtgagt tcgccggcga ggaattcgtc gagattgaac tcgttggaag ccgcctgcac
    12961 caattcgatt tcgtcgcgca actgttggat ttccaatgga aagcgttgtt ccaattcggg
    13021 attttctatg cctttgatga tgtcgaactc gtgcggcagg cgttcgccgc ccgcttcaaa
    13081 gagatagatt tcgtcgttca ggatgtggta cacgcctttg aagtttttgc ccataccgat
    13141 tggccaggta acgggcgcgc agcggatttt taaaatgttt tccacttcgt ctaaaagttc
    13201 cagggaatcg cgcacttcgc ggtcgtattt gttcatgaac gtaacaatcg gcgtatcgcg
    13261 caggcggcag acgttcaaga gtttgatggt ttgcgcttcc acgccttttg ccgcgtcgat
    13321 gaccatcaac gcgctgtcca cggcggtcaa aacgcggtag gtgtcttctg agaaatcttg
    13381 gtgtcccggc gtgtccaaga ggttgacggt gtggtcttta tagtcgaact gcatcacgct
    13441 tgatgccacg gaaatgccgc gctgcttctc gatgtccatc cagtcggagg tggcgaattt
    13501 gccggttttc ttgcctttta ccgtacccgc gctttgaatc gcacctgaaa acagcaagag
    13561 tttttcagtc aacgtggttt tacctgcgtc agggtgggag atgatggcaa acgtgcggcg
    13621 gcggcgcact tggtcgagga tttcttggga catggttttc tttgcaaaaa ggttcaggcc
    13681 gcttttcaga cggcccggac agtgtttgag acggcgaaat tgtacaaaaa aatgcctgat
    13741 aattcaatgt tggaggcggt cagtgcgtgc tgccgtaaat ctctttttcg tctttcagga
    13801 cggcatcggc ggtttcccac gcgctgccac gccagacttt gtaaaagcag ctttctcgcc
    13861 cggtgtggca ggcgatgccg ccgttttggg cgatgagcat cacaatggcg tcgccgtcgc
    13921 agtcgaggcg cagttcgcgg actttttgcg tgtgtcccga ctcttcgccc ttcatccatt
    13981 gtttttggcg cgaacggctg taatagtggg caaagccggt ttcgacggtt ttttgcaggg
    14041 cttcggcgtt catccacgcc accattaaaa cacgtttggt ttcggcatct tgggcgatgg
    14101 cgcaaaccaa acctttttcg tcaaatttga cggcttcaag caggttttta tccatatttc
    14161 ctttcagacg gcatagtcgg agcggtcaga ggcgcacttc gatgccggct tcgcgcatag
    14221 cgcgtttggc ttcgcggatg gcgatttccc cgaaatggaa aatgccggcg gcaagtacgg
    14281 catcggcttt gccttcggtt ataccttcaa tcaggtgccg gacattgccg acccctccgg
    14341 aggcgatgac ggggatgtcg acggcttcgg caacggcgcg ggtcagcggc aggttgaaac
    14401 cctgtttcgt accgtccctg tccataccgg taagcaggat ttcgcccgcg ccgcgttttt
    14461 gcatttcgat cgcccattcc accgcatcca aaccggtcgg attgcgcccg ccgtgggtaa
    14521 agatttccca gcgtgtgttt tcggagttga cggctttggc atcgacggcg gcgacgatgg
    14581 cttgcgaacc gaaaaatccg gcggcttcgt caatcaggtc gggacgggta acggcggcgg
    14641 tgttgatgct gactttgtcc gcgccggcat tgagcaggcg gcggatgtcg gcaacggtgc
    14701 gtacgccgcc gccgacggtc agcgggatga agacttgtcc ggcaacttct tcgatgatgt
    14761 gcaggatggt gtcgcggttg tcggatgagg cggtgatgtc gaggaaggtc aattcgtccg
    14821 cgccttcgcc gttgtagcgt ttggcggctt cgacagggtc gcccgcgtcg cgcaaaccga
    14881 tgaagttcac gcctttgacg acgcgtccgt cttttacgtc gagacagggg atgatgcgtt
    14941 ttgccagtgc cataatcgga tgcctttagt cgagggaatc tgccagttgc tgcgcttggg
    15001 caaaatcgat gctgccctcg taaatcgcgc ggccggtaat cgcgcctgct acgccatgtt
    15061 tttcggcggc acacagggcg cggatgtcgt ccaagccggt cagtccgccg gaggcgatga
    15121 cgggaatgcg gacggcttgg gcgagtttga ccgtcgcgtc gatgttcacg ccgctcatca
    15181 taccgtcgcg cccgatgtcg gtgtagatga tgctgttgac accgtcgtct tcaaagcgtt
    15241 ttgccaaatc aattacatga tgcccggtta cggttgccca gccgtcgatg gcggccatac
    15301 cgtctttggc atccagcccg acgataatcc tgccggggaa ggctttgcac gcctcgcgca
    15361 cgaagtcggg gtttttgact gccgccgtgc cgataatcac gtcgtttaag cccaaatcca
    15421 aatattgtcc gatggttttc aaatcgcgta tgccgccgcc gagctgtacg gggatgtctt
    15481 tggcgacggc ggcaaggatg tctttgatgg cgggcaggtt ttgcggaaca ccggcaaacg
    15541 cgccgttcaa atctaccaga tgcaggcggc gcgcgccttg ttcgaaccag tgcagcgcgg
    15601 tttcggcggg cgaatcggaa aagacggtcg cctcttccat cagcccttgt ctcaggcgga
    15661 cgcagtgtcc ttctttcaaa tcgatggcgg gtatcagcag cataattttt ctccttgtgc
    15721 ggggccgtgt ccggcttacc agtttaaaaa gtttttcaac atcgtcagtc cggcatcgtg
    15781 gcttttttcg gtgtgaaatt gcgtggcgaa tacgttgtct ttgccgacga tgcaggcaaa
    15841 cggggacggg tagtcgcttt cgcccaatat ggtttcggga ttttcggggg cgaaatagta
    15901 gctgtggacg aagtaaaaac gcgtgtcttg gggaatatct ttaaacagcg ggtggttttg
    15961 ggtttggcgc acggtgttcc agcccatatg agggactttc agacggcatc cctgccggtc
    16021 gcggaggtcg cgggcaaagc gtctgacttt gccgccgaac cagcccaagc cgtcggtgtt
    16081 tccttcttca ctgtggtcga ataaaagttg cgcgccgacg cagattccga aaaagggttt
    16141 gttttttaaa gcatctttga ctgcctcgtc caagccgtcg cgcgtcagtg ccgccataca
    16201 gtcgggcatc gcgccctgac cgggaaaaat gactttgtcg gcgcgggaca cgcggtcggg
    16261 gtcgccgctt aaaaagattt cggtattttt tccggcaagc tgccccgccg tccggacgga
    16321 tttcaatacg gaatgcaggt tgcccatacc gtaatcgata atggcggttt gcatggcttc
    16381 ctcctctttt tttgcaatat ggctgcgatt ttaacaaaca aatgtgccgt gctgataaaa
    16441 atgccgtctg aaaacgggag tctgtcttca gacggcatag ggtttaaacc cggaaagccg
    16501 tttgtcagcc ttccatttgt tttgcctgaa cggcagtcag ggcgatggtg aacacgatgt
    16561 cttccaccag cgcgccgcgc gacaggtcgt tgaccggttt gcgcaggcct tgcagcagcg
    16621 ggccgacgct taagacgttg gcactgcgtt ggacggcttt ataggtgcag ttgccggtgt
    16681 tcaggtcggg gaagaccaaa acggttgcct gtcctgccac cgggctgccc ggagctttgg
    16741 atttgcccac gcccggcacg gttgccgcat catattgcag cgggccgtcg atggcgaggt
    16801 cggggcgttt ttcccgggca agtttggttg cttcgatgac gatatcgaca tcggggccgc
    16861 tgccggagtt gacggtggag taggaaatca tcgccacttt cgggtcgatg ccgaaggctt
    16921 ttgcggaatc ggcagactgg atggcgatgt cggcaagctg ttgcgcggtc gggttcggat
    16981 taaccgcgca gtcgccgaag acgaggactt ggttgggcag cagcataaag aatacgctgg
    17041 acacgaggct tgcgcccggt gcggttttaa tcagttgcaa agcggggcgg atggtgttgg
    17101 cggtggtgtg aaccgcaccg gataccaaac cgtccacatc attttgcgcc atcatcatcg
    17161 taccgagtac cacggtgtct tgcagttgct tgcgcgcgtc ttcgggtgtc aggcctttgg
    17221 atttgcgcag ttcgcacatc ggctcgacgt attgttcgac caatgaggcg ggatcgatga
    17281 tttccaaaga gtcgggcagg ctgatgccgc gttctttggc aacggcttcg acttcttcgc
    17341 gtttggcaag caggacgcag cgggcaatgc ctttttcgtg gcagatggcg gcggcttgga
    17401 cggtgcgggg ttctgcgcct tcaggcagga cgatgcgttt gtcggcttgg cgggcgaagt
    17461 cgatcaggtt gtagcggaat tgcgccggcg acaggcgttt tgcttcgcgg cctgccaata
    17521 cggatacgtc tttcagcgcg tcgctcgaac cgaagaaggt caggccggtt ttttcggctg
    17581 ccgcttcggc aacggaggct gccgcgccgt ccacgacaaa accttccaat acgcccggcg
    17641 cggcggcgaa gaactgtttg gcaaggttca cccgatttgc cagttcgtcg gcatcggcgt
    17701 tgtcggaacg gacggcgaag acggctgccg cgtcaaggga caatgccagt tcgacgtttt
    17761 tgcctgcgag gtagattttg tcggcatcgg gcgcgatgcc ttcgatgacg aggttggcgg
    17821 catcgagtgc ggcaactttg ccgaccagtg cgtcgaacca gtcgtcgctt ttgccttgcg
    17881 cgagcagggt ttcggcggtt gcgtcaacgg cttggaaaat ttgtgcgtcc agtgcttttg
    17941 caaaggcttg tgcggcggcg gaggcgtcca gtccggcaga tacgggtacg atgagtactt
    18001 ttgccatgat atatcctttc gtatgctgcg gtgtgcggca tatgtggttg gaaggggcgg
    18061 catataggca gaaacggctg cctgcgtgcc gtgcgtgccg tgtttggctt gaggcgcgca
    18121 ggttgaatat agcaaacaaa ttctgtttcc aacaagataa atatccgcag gcttgtggat
    18181 gctgccgcct ttcagagggt atttccgggg aagaacaggg cgggaccgtc caaatggagg
    18241 acggcggaaa tgccgtctga cagggtgggg gcggaaggga ggttgagcgt gaggacggtt
    18301 tgtccgaccc ggaggctgat ttcggtatgc cgcgctttgg gcgtggtttt gagaaccacg
    18361 gcgtgaatgg aggcggtggg tgcggaatgg gggtgaaggc tgaactgttc cggacggatg
    18421 agcagtgtgc cgcgtgtgcc tgcgggtgcg ccgctttgaa cgggcaggcg gcccaatccg
    18481 caatcggcgg tgccgtcggc gttgagcgcg gcggggaaca cgatgccttc gccgataaac
    18541 agggcggcat caaggtcggc aggttgtcgg tacaattcgt gagggcttgc agtttggagg
    18601 atgcgcccct gtttcatcac ggcaatccgg tcggcgtatt gcagggcttc ttcgcggtcg
    18661 tggctgacga aaacggcaga tttgccgttg gcgcgcaggg cggcaatcat gtcttcgcga
    18721 atctggcggc gcaactgttc gtccagcgcg ctgaagggtt cgtccaacaa aatcagttcg
    18781 ggatcgggtg cgagggcgcg ggcgagggcg acgcgctgtt gctgtccgcc cgaaagttcg
    18841 tgcggatagc gtccggcaag ttcggaaatg ccggtcaatt ccaacatagc ttcgatgcgc
    18901 tgccgctctt gcgccgtctt gcctttgccg ttgcccagcc cgtaggcggt gttgcggtaa
    18961 acggtcaggt gggggaacag cacaccttcc tgtacgacat aacccaaacg gcgttcgcgg
    19021 acggggaggt tggtattttt cgagaagatg gttctgccgg aaagcgaaat ttcgccaaaa
    19081 tcgggttgtt caaaaccggc aaggcagcgt aaaagggtgg ttttgccgca gccggacgcg
    19141 ccgacgataa agaggatttc gcccgggtcg aggctgagcg aaatgtcgtt taaaaccggg
    19201 gtgttttgaa aacttttgga caggtgtccg atgtgcaggg cggcggtcat ggcggtactt
    19261 cctcaagctg ttatttgaag gcgtatttct tcagcaggaa tacggggatg ccggaaaata
    19321 ataccagcat cagcgcgtaa ggggtggcgg cggcgtattg tgcgtctgat gtgtattccc
    19381 aaacggcggt ggagagtgtg tggacatcgt cggtggtcag cagcagggtg gcggtcagct
    19441 ctttcatcag tttgaggaag acgagtgcga atgcggcggt aatgccgggc aggatggacg
    19501 gcagtaccaa cgtcctgaaa ataaagaagt gtccgcgccc caatgttgcg ccgacctgtt
    19561 ccatcccttt tgggagttgt tccaaggaag tcctcagggt ggtttgcgcc atcggcaggt
    19621 aaagcatgaa ataggcaagg atgacgacga taaaggtttg gtaaacggca ggggtgtagt
    19681 tgatgctgaa ataaaccaag gatagggcga taaccaaacc ggggacggcg tgcagtaaaa
    19741 acggcagcct gtctatccaa acggttaaaa aattgcgata gcgaaccgat gcccaaacaa
    19801 ggggcaaggc acataatata gtcaaaatcg cacctaaagc cgatacgctt aaggaacgga
    19861 taaaggcatc aaatacggat acgagcgcga atgtgccgga agtgccgacc atcatccaat
    19921 gtatcaatac gccaaagggg ataataatgc ccaaagtcaa caagctgctt aaaaaaacaa
    19981 tcgcgccaat ctgaccgggc agtttgaggg ttttgacggg ataaggacgg gcaacgcctt
    20041 tgccgctgtg gtaaatcttg gctttgccgc gaaatatgct ttctccaaat acgacgatgc
    20101 cgcacaccgc cattaaaaca gcggaaagca gggcggcggt attgttgttg taggacattt
    20161 cgtattcttg gaaaatggcg gtggtaaaag tggggtagtt caaaatggat accgcgccaa
    20221 attcgaccaa catatgcagg gcaatcagta acacgctgct gccgatggcg ggtttgagct
    20281 gggggaggat ggcggaaaaa aaggtttgca ggcggctttt gcccaaggac aggctgactt
    20341 cttcgtaaga caggctgatg cgtttgagtg ccgcctcgac gggcaggtag gcgagcggga
    20401 acgaggacag gctcataatc atcactgtcc cccaaaagcc ttcgacacgg aaggtcaggc
    20461 tgatccaggt gaaacagctg acaaatgcgg ggatgcacaa aggcagggtg attgccgtct
    20521 gaaaaaaggt tttgccgaag aagcggtaac gttggaacaa aagggcgcag gcaatgccca
    20581 aaacaatgga aatcagggta acgcccgcca tcatcgtcaa ggtgttggag agcaaatccc
    20641 acatacgcgg gcggaacaac agttcgacgg cgcggttgat gccgacctgc cacgaacgca
    20701 tagcgacata taaaaaaggc agggtaagcg gtagggcaat cagtaggatg aggccggtaa
    20761 gccaaatggg tattttttta ggagacatag tgttttttat cggcaaaacg ggcggacagt
    20821 ataaatgtcc acccgtttga caatccgaaa acggcttatt tcataccggc ttgctcaagc
    20881 agccgggtgg cgtgttcttt ttcggaaaca gtggtggcgg acacttgggg tgcttccaac
    20941 ttggcgatgg gttccaaatt gaaggtggat accacgtgcg gattcaaagg atactcggca
    21001 cggacggcgg tcagggcgcg ctgtccttcc ttgctggcga ggaaggcgac gaattttttc
    21061 gcctcatcct tgttttggga ggattttaac acggctgcgc cggaataggt aatgagtgcg
    21121 ccgggatctc tgtggcggac gaaattcagg cgggtgtgga cattttgtac gcctttttca
    21181 cgcgcgaaag cgtgccagta gtagttgttg atgagggcgg catcgatttc gccgttttca
    21241 accgcttgaa gggcgacgga gtttttagcg taaggcttgc cgtattcttt cagacctttg
    21301 agccatttca atgcggccgc ttcgcctttc agtttgacga tggcgacaac ctgttccaag
    21361 aacgcgccgg aagtgggggc gtaaccgatg cggtttttcc atttcggcgt ggcgtaattc
    21421 aggacggatt tttccaaatc tttttcagac agtttgcggg tgtcgtaaac gacgacgcgc
    21481 gaacgtccgc tcagtgccac ccagtctttt ttggcggcaa ccggcacgcc cttgccgcgt
    21541 gtttcgttga tggtggaggc gggcaggggc tctaggaggt tggctgcgga aagggtggcg
    21601 agtgccggga tttgttcgga atagaatacg tcggcggggc ttcggctgcc ttcttctttg
    21661 atttgaccgg caagctggtc gcctttggcg ctgttgagtt tgactttgat gccggtagcc
    21721 cgggtaaagg catctgcaac ggcttgtgct gcttctttgt gttgtccgtt gtacacggta
    21781 atgtctgcca gcgcgggggt tgcggcggtc agggctgcgg caagcagtgc gtatcggata
    21841 gatgttttca tatcgatttt ctcctaatga atgagagtgt acaccttgtt aagacataac
    21901 ggcgtgtagt gtattccttc ttctttataa atgcaaataa ttatttttta aatttgttat
    21961 tatccgatcc ggttatggtt tgttctgact tgtatttttt ccgtgcatcg cgcccgtaag
    22021 gcggaagtgg cgggcaatgc gtggcggaat gtgggtaaag gcggcatttt gatttgtcgg
    22081 aatgcttgag aacccctctc tttaaaacac cgtctgaaca gggttgctgg aatagtattg
    22141 ccatcccagc agatacagtt tgtcggggtc ttgccaatat tgttcatcca gactgttcgg
    22201 cagtgaggcg gttttgttgt cgagatgtta tatcccacat ttctttcagg tttttacctt
    22261 ccgattggag gcggcggatt tctttgtcca atgcgtctgc cgcccgatgg atcagcattg
    22321 cgctgtgtat cgcgccgatt tttttcagaa cggaactagt cttttcggcg gcgggaaaac
    22381 accagttgca gacaaattgc agtatgccgc cgttaccgat gtcggcttcc gcccgccaaa
    22441 tcaaaaccag ctcctgttcc cgtccgtcca tgctttcgag tttgccgtta tgctgttcaa
    22501 aaagtttgtc caccgcctgc cacatcatct gctcgaagcg gtcggcttgg gtatccgtat
    22561 cggtcatcgt gttcttgcct tttaaaaatg ccgtctgaac atttcttcag acggcatttg
    22621 ggggttaagc caacatttcc cgccagcgtt tcacttggaa gcggacttgt tccggcgcgg
    22681 taccgcccaa gtggttgcgg gcgtttaagc tgccttcggg tgtcagcacg ccgtaaacgt
    22741 cgtcggcaat caaatcgctg aaaccttgta agacttcgag cggcagttcg ctcaaatcga
    22801 cgcccgcttg gtcggcgtgg cgcacggctt gggcgacgac ttcgtgggca tcgcggaaag
    22861 gcatgccttt tttgaccaga taatccgcca agtcggtggc ggtagcgaag ccctgcatca
    22921 cggcggcgcg catattgtcg ggtttgacgg ttacgccgcg catcatatcg gcgtaaatcc
    22981 gcaacgtgtc gataagcgtg tcggcggtgt cgaacaaggg ttctttgtct tcctgattgt
    23041 ctttgttgta cgccaagggt tgggatttca tcagggtaat cagaccgata aggtgtccga
    23101 tgacgcggcc ggatttgccg cgcacgagtt cgggcacgtc ggggtttttc ttctgcggca
    23161 tgatggacga acctgtgcag aaacggtcgg cgatgtcgat aaagccgaaa cgcggactca
    23221 tccacaaaat caattcttca gacaggcggc tcaggtgaac cataaccagc gaggcggcgg
    23281 cggtgaactc gatggcaaaa tcgcggtcgg atacggcatc gagcgagttt tggcagattt
    23341 gttcaaagcc caacagttcg gcggtgattt cgcgccgaat cggataagtc gtaccggcaa
    23401 gggcggctgc gccgagcggc atacggttga cgcggcggcg gcagtccgcc atccgttcgt
    23461 tatcgcgtcc gagcatttcg acgtaggcga gcatatggtg tccgaagctg acgggctggg
    23521 cgacttgcag gtgggtaaag cctggcatga cggtttcggc gttttgttcc gccaaatcca
    23581 gcaatgccgt ctgaaggctt tgaatcaggc ttcggatgac ggtaatctgg tcgcgcagcc
    23641 aaaggcggat gtcggtggcg acttggtcgt tgcggctgcg gccggtgtgc aggcgtttgc
    23701 ccgcgtcgcc gattttgtcg gtcaggcggc gttcgatgtt catgtggaca tcttccaaat
    23761 cggacgacca ttcgattttg ccgctgcgga tttcttcgag gatttccgcc ataccccgac
    23821 ggatgtctgc caaatcgcct tcgtccaaca cgccggtttc tttcaacatt tgcgcgtgtg
    23881 ccagcgagcc ttgaatgtcc cattcggcaa gccgtcggtc gaaaccgatg gaggcggtgt
    23941 attgtttgac gagttcggaa acgggttcgt tgaaacgtcc ggaccaggtt ttgtcgtgca
    24001 taaggattcc ttgatggggt tattcggtac ggtatttttc caaaaggcgg cggaagggtt
    24061 cgccggtttc ggggtgtttc agaccgtagg cgacggtggc ttcgaggtag cccagtttgc
    24121 tgccgcagtc gtagcgcgtg ccttcaaagg ggtgcgccag gacaaattcg tgatcgagca
    24181 gcttggcgat gccgtctgta agctggattt cgttgcccgc gccgcgcggc agtccggtca
    24241 gtaagtcgaa aatgcgcggg gtgaggatgt agcgtccaac aacggcaagg ttggagggcg
    24301 cgtcttcggg cttgggtttt tcgacaatgc cggtaatgcg ttggaactgt ttgagctgtt
    24361 cggtttcgac gatgccgtat gagccggttt gcgatgcttc aacggtttct acgcccaaaa
    24421 tgctgttgcc gctgcgcccg tacacttcga ccatttgttt gagcgcgcct ttgggggcat
    24481 caatcaggtc gtcggcaagg ataacggcaa agggttcgtc tccgatggcg gcgcgggcgc
    24541 acaagacggc gtgtcccaag cccagtgctt ccgcctgacg gatgtagagg caggtaatgt
    24601 tcggcggcag gatgttgcgg acgtgttcca acaatttgtc tttatggcgc atttccaact
    24661 cggtttcgag ttcgtatgcc ttgtcgaaat ggtcttcgat gctgcgtttg ttgcgtccgg
    24721 ttacaaacac catttccgtg cagccggctt ccacggcttc ttccacggcg tattggatca
    24781 gcggcttgtc gacgatgggc agcatttctt tcgggcttgc cttggtggcg ggcaggaagc
    24841 gggttcccat ccctgcgacg gggaagacgg ctttccgtat gggtttcatt ctttttcctt
    24901 tgtattgttt tgatgtttaa agggcgagtt tgcgtaagag ttcggcaagt gcctgcgcgc
    24961 ggtggctttc gcggtttttg acctccgaat ccaattcggc ggcggttttg ccgtgtccgg
    25021 gcagataaaa atacgggtcg taaccgaaac cgttttcacc ggcgggaacg ccgttccatt
    25081 gtccgtgcca tacgccctcg gcgataattg gacgcgggtc gtctttatgg cggacaaaaa
    25141 ccaatacgca gacgtagcag cagcttttgt cggtcttgcc ggcaagttcg gcggcaagtt
    25201 tcaggttgtt ggcggtatcg gatttgggat tgtcgcccgc gtaacgtgcg gaatggatgc
    25261 ccggcgcgcc gtttagggcg gcggcacaga tgccgctgtc gtcggcaagc gcgggcagcc
    25321 cgctgtattc ggcggcatgg cgtgcttttg ccagcgcgtt ttcgacaaag gtgggatagg
    25381 gttcggggca ttcgggtatg ccgaatgcgg attgcggcaa tacggtgatg ctgtaaggtt
    25441 tgaataagtt gccgaattct tcgagcttgc cggcattgcc gcttgccaaa acgatttttt
    25501 ccggtttttc agacatagcg gttttccttt gcggcggatt gggcggcgcg tagggatttg
    25561 tgccgcaggt agagggcgag gctgccgatt tgtccgaaca gtgcgccgaa tgcgaaaaga
    25621 aaggaggcga cggcgaaggc tttgctgccg acggtcagca gataagcacc caagaggacg
    25681 agcagcataa atgccagtgt gaagagggca acagacaaca gatagatttt tcggcggttc
    25741 atggcgttcg gtcggaaacg gtatgttcgg attatagccg attgggacgg tattccctag
    25801 agcttggaaa aatgccgtct gaaacggcgt tcagacggca tggcgggcgt tatgcctgtt
    25861 tgttccaacg ttcgatggat tctttgatga cttttttcgc ttcttccgca tcgccccacg
    25921 attcgacttt ggtcgtacct gcttttttca ggtctttgta atggttgaag tggaactcga
    25981 tttgtttgat cagctgttgc ggcaaatcgg acaaagtttt gtaggcgttg ccgttgttgc
    26041 ggtcgtcggc aggaacgcag acgattttgt cgtctacttc gccgtcgtca acgaatttca
    26101 tcacgccgat aacgcgcgct tccaagaata cgccggttgc cagaggttgt tcggtaacga
    26161 gcagcacgtc caattcatcg ccgtcttcgt ccaaagtttg gggaatgaag ccgtagttgg
    26221 tcggtttggc gaagatggcg ggttcgacgc ggtcgagttg gaatgcggcg agtttgcggt
    26281 tccactcgat tttgtggttg ctgccggcgg ggatttcgtt gacgacgttg atgatgccgc
    26341 cgtccacatc gccgggggtc aggatttggt tgaagtctgc catttggttt cctttgtttg
    26401 ggaaagtttg aagtttgaaa gtatagcaca aacgtccggt tgaaaatgcg cccgatgccg
    26461 tctgaaaggg tgtacgggcg cgtgttaccg tttgcccaaa aacctgccca gttccaaaat
    26521 tgcgcgcctg ttggacgggg aaaacacttt ttccgccgct tcttccaagc cgaaccagcc
    26581 gtaggagacg tgttcctcgg gttgcaggac gacgggcgta tcgcgcggga tttcggcaga
    26641 gaagaggtgt tcgcggtttt caaacacgcc tttggggtag cggtgccgcc agtggtggta
    26701 gatttcgtaa accgtgctgt cgtgccagtc ttgaagctgc ccgtccgcca gcaggatgcc
    26761 ggtttcttcc caaacttcgc gccttgccgt ttgggcgacg gtttcgcccg gttcgaggct
    26821 gccggttacc gactgccaaa atccttccgg atgcgtgcgt tcgatgagca ggatgccgcc
    26881 gtccccgcta taaaggacga ccagtgcgga gacggggtat ttgagcggtt ttgccatcgg
    26941 catctttcgg cgggctgcgg taatgaaggg gcggattata gcaaacgccg cacgttatgg
    27001 cgtttatcct tttccgtatc cttttttctg cacggatggg acgcgccggt gtttgccggt
    27061 aaattttccg ttgtgtcaaa aagataaggg aggttgtgat tttaatgctt gccaaagcgt
    27121 cgggcggaaa ctataatccg aaacttatcg agtcggagtg tggcgcagtc tggtagcgca
    27181 cttgcatggg gtgcaagggg tcgaaggttc gaatcctttc actccgacca aaaattccga
    27241 aagccgcttt caaaagcggc ttttttgccg tccgtatgat tatgatgtag agtacgcggc
    27301 gacagacatt caaatgccgt ccgaaaaccg ttcagatggc atctctttca tcttagtttc
    27361 attccgtacc atcttaagga acatcaaatt gggcatttcc cgcataatat cccttattct
    27421 gtccatactg gcagtgtgcc tgccgatgca tgcacacgcc tcagatttgg caaacgatcc
    27481 ttttatccgg caggttctcg accgtcagca tttcgaaccc gacgggaaat accacctatt
    27541 cggcagcagg ggggaacttg ccgagcgcag cggccatatc ggattgggaa aaatacaaag
    27601 ccatcagttg ggcaacctga tgattcaaca ggcggccatt aaaggaaata tcggctacat
    27661 tgtccgcttt tccgatcacg ggcacgaagt ccattccccc ttcgacaacc atgcctcaca
    27721 ttccgattct gatgaagccg gtagtcccgt tgacggattt agcctttacc gcatccattg
    27781 ggacggatac gaacaccatc ccgctgacgg ctatgacggg ccacagggcg gcggctatcc
    27841 cgctcccaaa ggcgcgaggg atatatacag ctacgacata aaaggcgttg cccaaaatat
    27901 ccgcctcaac ctgaccgaca accgcagcac cggacaacgg cttgccgacc gtttccacaa
    27961 tgccggtagt atgctgacgc aaggagtagg cgacggattc aaacgcgcca cccgatacag
    28021 ccccgagctg gacagatcgg gcaatgccgc cgaagccttc aacggcactg cagatatcgt
    28081 taaaaacatc atcggcgcgg caggagaaat tgtcggcgca ggcgatgccg tgcagggcat
    28141 aagcgaaggc tcaaacattg ctgtcatgca cggcttgggt ctgctttcca ccgaaaacaa
    28201 gatggcgcgc atcaacgatt tggcagatat ggcgcaactc aaagactatg ccgcagcagc
    28261 catccgcgat tgggcagtcc aaaaccccaa tgccgcacaa ggcatagaag ccgtcagcaa
    28321 tatctttatg gcagccatcc ccatcaaagg gattggagct gttcggggaa aatacggctt
    28381 gggcggcatc acggcacatc ctatcaagcg gtcgcagatg ggcgcgatcg cattgccgaa
    28441 agggaaatcc gccgtcagcg acaattttgc cgatgcggca tacgccaaat acccgtcccc
    28501 ttaccattcc cgaaatatcc gttcaaactt ggagcagcgt tacggcaaag aaaacatcac
    28561 ctcctcaacc gtgccgccgt caaacggcaa aaatgtcaaa ctggcagacc aacgccaccc
    28621 gaagacaggc gtaccgtttg acggtaaagg gtttccgaat tttgagaagc acgtgaaata
    28681 tgatacgaag ctcgatattc aagaattatc ggggggcggt atacctaagg ctaagcctgt
    28741 gtttgatgcg aaaccgagat gggaggttga taggaagctt aataaattga caactcgtga
    28801 gcaggtggag aaaaatgttc aggaaataag gaacggtaat ataaacagta actttagcca
    28861 acatgctcaa ctagagaggg aaattaaaaa attaagaact tcagaaggaa aggcaaattc
    28921 tgcatcagca gctttattga cacttgattt gaagacagct caagctgcca atcaagttgt
    28981 tgaaagtttg agaagtacag gtaatcttcc ttctaattat attactaaat ctcaggcgat
    29041 aagtatggga tggcgtccag gaaaagcctt gggtaataca ataaaaaatg gacaattagg
    29101 gggagatatt tttgcaaatt ctacaaatat tttacctgct aaacagggta ggatatggta
    29161 tgaagcggac attggcttaa taaataccat gtcaagatcg aaccaggctg gtactagatt
    29221 attatattct aacgatggat tgctatatat aacaactgat cattatatct ctgcaataca
    29281 aattggaact tggaaatagg agtataactt atgcaattag agattatcgg tagtaaaatt
    29341 tatacggaac aagattttca taatcaaatt tcaaaaatat tttctataca agattattat
    29401 gggaacaatc ttgatgcttt atgggattta ttaagcacaa atgtagaacg accgattact
    29461 ttggtatgga aagatgctat gttctcaaaa aatcaattag aaaatatatt tattgaaatc
    29521 gtaaatgttc tagaaagagt taagaaacaa gatgaggatt atggattcga agaaaaattt
    29581 aattatattt tagagtaagt aaaccctaat tacattacgt acacaggctt aaaactcccc
    29641 agagccaatt aagcaagccg taacccatat aaaacttaaa ctcaacaagt agcatgtgtg
    29701 cggaacgtac gcatgcggtt cttaaagttt gagctaagag gccgtctaaa aacagaaaaa
    29761 ccgtttcaga cggtctttgt ttaacgccac cgatccagcg ggttacaaag cgcagtcaat
    29821 gccgctgcgc cttatgcctc cgaagcaata ggcagaacat ttggacacgg tgaaaacaaa
    29881 aacgaaaccg cccaagccgt cggacatttc cttttaggag cagctattgc ccgcgtcaac
    29941 ggtggtaatt ttgctgccgg cggctcggca gcagttgcag ctgaaaaggc ggcggaacat
    30001 cttgcccaac agtataacga cggtaaaacc gcaatcgatc cgcaaacagg cgagttcaat
    30061 gccaacctgc tgccggaaca tatcaaagag gaaatcaaat caaagagcgg ggtgattgca
    30121 tcgctgacgg gcgcggccgt gggcggcacg ccggtagatg cgcaaaccgg aggtgcggtc
    30181 ggacagaatg cggtggaaaa caacctctat ctgacatcgg aagccttaaa gaaggacaag
    30241 cagacagctc gtaaaattta ttccgtcata aaagagcaag tcaagcatga atgcagttcc
    30301 acaggaagaa ttaccgaatg tcgtcaaaat ataggacgca ttatcgaatt tacccaagac
    30361 aaacgctttg acagtaggtt taaggactta aaaaaagaat ccttatatta cctaaataaa
    30421 catcctgatt tagtagcctc ttatttgaag gctgaatacg aaaagctgga tagggaagac
    30481 aaaagtatcc tgcaccgcta catctcaccc ggggctgaaa tcgtttcggg cagtttgggg
    30541 gttgttcttt caggagtagc cggaggcgga tcttgtgccg agactttcgg cttaggctgt
    30601 gccgccgctt tggttggtgt aacgtcttcc tacgatcatg tcattactgg gacgaaaaac
    30661 ttcggaaaaa aagccagcga gcaacgaccg acgattgcgg ttcaggcctt gaagcagttg
    30721 gggctgtcgg agcaggctgc ggaatatgtt cagttctcta tagatttgtt cagtgtgggt
    30781 aaatcggggg gcggtatacc taaggctaag cctgtgtttg atgcgaaacc gagatgggag
    30841 gttgatagga agcttaataa attgacaact cgtgagcagg tggagaaaaa tgttcaggaa
    30901 acgagaagaa ggagtcagag tagtcagttt aaagcccata cgcaacgaga atgggaaaat
    30961 aaaacagggt tagattttaa tcattttata ggtggtgata tcaataagaa aggcacagta
    31021 acaggagggc atagtctaac ccgtggtgat gtacgggtga tacaacaaac ctcggcacct
    31081 gataaacatg gggtttatca agcgacagtg gaaattaaaa agcctgatgg aagttgggag
    31141 gtgaaaacga aaaaaggtgg gaaagtgatg accaagcaca ccatgttccc aaaagattgg
    31201 gatgaggcta gaattagggc tgaagttact tcggcttggg aaagtagaat aatgcttaag
    31261 gataataaat ggcagggtac aagtaaatcg ggtattaaaa tagaaggatt taccgaacct
    31321 aatagaacag catatcccat ttatgaatag taatatttat gaaaaattag gagattaatg
    31381 atgaaaagaa ttaagtgctt ttgtgataaa tttccatcag gagatacatt tagaatgtgt
    31441 atcattctgg atgactatga taatagggtt gattattatg taggaatata tgattacatt
    31501 acgtctacct taatgagcga tatttactat cgatccacga ttgatgagca tttcaagatt
    31561 atagaattaa tagaaaataa tccaaatgaa atttatgatg atggcggtgg tcaacaattt
    31621 tgcctagaat ttcatcatga taaggtcatt ttttatcaca atgaatttga tgaagaagat
    31681 ggttatccag tattaagctg ttcgctgcat acttttaaaa ctgctttaat tgcttggaat
    31741 gcttttttgc aattgcctaa aagtattcat tcggtggtgg agactgtgat tgaggaataa
    31801 gcataattag cttaatgaat agaatcagcg atatagaaag gtagaatcag atatatccca
    31861 cctaaaaatt actctccttc agcaccgcta ccaaaaggac ctaataatgg atatagtgcc
    31921 gggaatttaa gcaaaaaagt cctgacttta gtttacataa acggcaaact caaattcggg
    31981 ccaagcagtc tgccgtcttc ggcattgaaa gcacctgttc ccattgtcgg caaggctgag
    32041 gctccgcccg gaaaagctgt gcctgtcggc gaagcccgca ttccttcaaa tccaaaagcg
    32101 gtaaaaggaa caaccgttac cctgtcgcag gaacaacgcc tagccaacgg agagctgata
    32161 ccgaaaggct ctgtggtaac gtggtcggac aatgcggtga aggttgtgcg gcctgatggg
    32221 aaaaagcgga gatttaatag tcgggattgg gaacagaagg cattgccgaa accgcaacta
    32281 ccgaaagatt acagagatga catcaacata ggtggaaata atcctagatt taatcagcaa
    32341 cggcatgaaa aaatcgaatc ccacaaccca aacagaatag aggagatacg ccaacgtaat
    32401 gccgtttctt ctgcagaaag gcaggctgat gcattaaaaa ggatgcaaac gtctggccgc
    32461 acccgcctgc ctgggcagcc acaaaaagga aaacccggcg ctgctagaga aatgtcctct
    32521 tcagacaatc cgaatcaaac agccgaggct tttgcacgtc gatatttagg caggaaacct
    32581 acactaatgg aacgagtcga aggtgaaaaa ttatacggag gtaaatgtgt gggctgttgg
    32641 aaagcgcaaa tgccggacgg aacctttgtg gtgtataggc cagcaggtaa tgcttctatg
    32701 accaagggaa ctactgcgtc tgttgatatt aataacccaa cattaaataa cttaaatatt
    32761 aatttaaaag gcaagccaac agagttaaaa tttaaattcc ctgctagata aggatgtttt
    32821 aatgaattta gagacagcag aaaatattca atgcgaattt gagtatttag cattagatgg
    32881 ttattttcca atgcattttg catcgcatgg tcaaggaaac aaagattggc aatttgcagt
    32941 tgaattcata tatagattat taatatgtca attagcaact cttgaaccta tcaactttga
    33001 aacgaaaaat gatattttag acttttgcca taatttagca aaacaaagtc cttttaataa
    33061 tgataatgag gtgtggtatc agggggaaat tgtattaaca aaaaaaggga tagatttaat
    33121 aaaggaatat attccagagg cgtttgaaga gtggaatgga aagaaatttg aattaaacat
    33181 tcctttcatt aaaacattaa aaaatatatt tgttaattat gaggtagcat gggatgagaa
    33241 taatccacta ttccccatta tatctttaaa cctcggcacc tgataaacat ggggtttatc
    33301 aagcgacagt ggaaattaaa aagcctgatg gaagttggga aatgaaaaaa ggaaggggcg
    33361 gaaaacctat gacaaatcat tctatgttcc caaaagattg ggatgaggct agaattaggg
    33421 ctgaagttac ttcggcttgg gagaaaagaa aggaattaga aggtcaaaaa tggagaggta
    33481 caagtaaatc tggtgttgaa atagaaggat ttacggaacc taatcgaaca gcttatccac
    33541 tttataatag taaatgaagt attgattaaa aaggtgtttt taaatgtata aaataaaatg
    33601 tttttatgat cagtttagtt ctggtgaatt atttaactac tgccagtttt tagataattc
    33661 ttctaatcaa aagataaata cacgaggaac ggtgtatcaa tatat
//
LOCUS       DAWFJR010000015        31337 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWFJR010000015 DAWFJR010000000
VERSION     DAWFJR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31337
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.015"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(169..1869)
                     /gene="recJ"
                     /locus_tag="WGR04_000606"
     CDS             complement(169..1869)
                     /gene="recJ"
                     /locus_tag="WGR04_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ0794215.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAARRLADAVERQEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAEQGVDLLITVDNGIASIAGVARAQELGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLMALRAELRRRNYFSDGLKEP
                     NLGDLLDLVALGTVADVVSLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDVEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTSFEEAVRE
                     MVCEDDLSQTFITDGSLPACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAV
                     "
     gene            complement(1938..2651)
                     /locus_tag="WGR04_000607"
     CDS             complement(1938..2651)
                     /locus_tag="WGR04_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794216.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTAMMWGMNDPFSQTTAYKHVDKDQ
                     IRAFGVVAKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKQFQMVEPNPR
                     FAYQALPVKLESPASQNFSTDDLCLRYDTDRPADIAKLKQLEFEAVELDNRTIYTRCV
                     SAKGKYYATPQKLNADYHFEQSVPADIYYTVTKKHTDKSKLFENIAYTPTTLILDAAG
                     AVLVLPMAALIAAANSSDK"
     gene            complement(2698..3267)
                     /locus_tag="WGR04_000608"
     CDS             complement(2698..3267)
                     /locus_tag="WGR04_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ0794217.1"
                     /translation="MPSERGGILLKCAIGKNSRIKMFEQEDEWISKTQMKKQMNGLQD
                     LGMELTKLSNDTLKKIGLDEDLYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIE
                     AFLAKLRGDDAVHNAFLQRVEQARVRLLADDGALTQFMSDFPHADAGKLRTLIRNTKK
                     EQEQNKPPKNFRALFQELKTVMENGDAEI"
     gene            3358..4689
                     /gene="pmbA"
                     /locus_tag="WGR04_000609"
     CDS             3358..4689
                     /gene="pmbA"
                     /locus_tag="WGR04_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ0794218.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKATGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADACLMAKHIGDPDLYHEWDLDTEAAVELAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDATVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGVNAITGDYSRGAAGFWVEN
                     GIIAYPVHEITIAGRLQDMYRNIVGVSDDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(4987..5190)
                     /locus_tag="WGR04_000610"
     CDS             complement(4987..5190)
                     /locus_tag="WGR04_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ0794219.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            5433..5735
                     /gene="clpS"
                     /locus_tag="WGR04_000611"
     CDS             5433..5735
                     /gene="clpS"
                     /locus_tag="WGR04_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ0794220.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            5737..8034
                     /gene="clpA"
                     /locus_tag="WGR04_000612"
     CDS             5737..8034
                     /gene="clpA"
                     /locus_tag="WGR04_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ0794221.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDAAVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDEDDGNYSSDG
                     MDDDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPL
                     LVGEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKS
                     VLKQLEKIPQAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEY
                     RTIFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELS
                     ARYINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVSRIPEKTVSH
                     DDKQVLQFLGRDLKNMVYGQENAIDALVTAVKMSRSGLALPDKPIGSFLFSGPTGVGK
                     TEAAKQLAYSLGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHC
                     VLLLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSL
                     GFTAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHQLLD
                     KKVEAEFTPALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSDGGFVRI
                     DWDAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(8074..11172)
                     /locus_tag="WGR04_000613"
     CDS             complement(8074..11172)
                     /locus_tag="WGR04_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ0794222.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENQLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLSVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSKPESGGYVWHTTGSGKTLTSFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSESTAGLKRNLEKDDNKIIVTTIQKLNNLMKS
                     EDNLPVYHQRVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKKLSAAENRQALL
                     HPERIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLH
                     PLKVATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNQEIDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYIDDRTG
                     EARRGYLDVAKELRERFPEPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QSVDAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYNDIRDWLRRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEGLNETA
                     AKRYLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGT
                     DI"
     gene            complement(11209..11312)
                     /locus_tag="WGR04_000614"
                     /pseudo
     CDS             complement(11209..11312)
                     /locus_tag="WGR04_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(11357..12529)
                     /locus_tag="WGR04_000615"
     CDS             complement(11357..12529)
                     /locus_tag="WGR04_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ0794223.1"
                     /translation="MDMQNKAKKLIEMIQTAPVEWKLLTEVADLTRGRVISKGYLADN
                     LGNYPVYSSQTLNNGEIGKINSFDFDGEFVSWTTDGANAGTVFYRSGKFSITNVCGLI
                     KIKNILELNYKFLFYWLSIEAKKHVYSGMGNPKLMSHQMGKIQIPIPPLEIQQEIVKI
                     LDKFTELEATLEATLEAELTLRKRQYQYYRDFLLDFDNQIGGIADDYKGRLKNVVWKT
                     LGEVSDINVGKSPETNEIGNYPLINAGTIPSGYIASYNTEPDTITTPSRGQGGIGYVG
                     YQTSKFWCGPLCYRIRGNNNLIMTKFLYYVMSKDMSKIINLANMAGIPALNKKDLVNF
                     LIPIPPLAEQEKIVAILDKFDTLTHSISEGLPHEIALRRKQYEYYREQLLAFPKAT"
     gene            complement(12651..12743)
                     /locus_tag="WGR04_000616"
     CDS             complement(12651..12743)
                     /locus_tag="WGR04_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-damage-inducible D domain protein"
                     /protein_id="HEZ0794224.1"
                     /translation="MTTENNAFENAKHIDETGNEYWSARTLQTK"
     gene            complement(12860..14404)
                     /locus_tag="WGR04_000617"
     CDS             complement(12860..14404)
                     /locus_tag="WGR04_000617"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEZ0794225.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     EELDFGNFEDHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQNVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNQF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSINWIGSDDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSSRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDASGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNDYNLAVSSYVEAEDTREIIDIKQLNAEISETVAKIERLRHEIDEV
                     IAEIEA"
     gene            complement(14477..15481)
                     /gene="rfaD"
                     /locus_tag="WGR04_000618"
     CDS             complement(14477..15481)
                     /gene="rfaD"
                     /locus_tag="WGR04_000618"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ0794226.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKSGVERYVKWMLENLA
                     "
     gene            complement(15610..16641)
                     /locus_tag="WGR04_000619"
     CDS             complement(15610..16641)
                     /locus_tag="WGR04_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0794227.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPFIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKIIYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEILLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKN"
     gene            complement(16643..17877)
                     /gene="dcm"
                     /locus_tag="WGR04_000620"
                     /pseudo
     CDS             complement(16643..17877)
                     /gene="dcm"
                     /locus_tag="WGR04_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(17914..18885)
                     /gene="hldA"
                     /locus_tag="WGR04_000621"
     CDS             complement(17914..18885)
                     /gene="hldA"
                     /locus_tag="WGR04_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ0794228.1"
                     /translation="MPAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGDDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNREVLEQIKRKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHEGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNEGEL
                     TEKAQNLRRHLDLTAILLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGVG
                     LGLAAGYTMPEAMYLANTAAGVVVAKLGTAVCSFAELTKALSGQSTM"
     gene            complement(18941..19681)
                     /gene="pyrF"
                     /locus_tag="WGR04_000622"
     CDS             complement(18941..19681)
                     /gene="pyrF"
                     /locus_tag="WGR04_000622"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ0794229.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFANEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRSLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNIAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAADPLAVLREVNRVANLEAN"
     gene            complement(20207..20854)
                     /gene="adk"
                     /locus_tag="WGR04_000623"
     CDS             complement(20207..20854)
                     /gene="adk"
                     /locus_tag="WGR04_000623"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ0794230.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            21069..21908
                     /gene="htpX"
                     /locus_tag="WGR04_000624"
     CDS             21069..21908
                     /gene="htpX"
                     /locus_tag="WGR04_000624"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ0794231.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(22209..22769)
                     /locus_tag="WGR04_000625"
     CDS             complement(22209..22769)
                     /locus_tag="WGR04_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794232.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(22883..23275)
                     /locus_tag="WGR04_000626"
     CDS             complement(22883..23275)
                     /locus_tag="WGR04_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0794233.1"
                     /translation="MGKRMTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQ
                     LLYYDTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALN
                     TPYGSIRTKLDGRSNPDNLVCNRYKVLL"
     gene            complement(23483..23875)
                     /locus_tag="WGR04_000627"
     CDS             complement(23483..23875)
                     /locus_tag="WGR04_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0794234.1"
                     /translation="MAKSFNQAASELTDIFPNISLTGFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(23992..24402)
                     /locus_tag="WGR04_000628"
     CDS             complement(23992..24402)
                     /locus_tag="WGR04_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0794235.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(24416..24853)
                     /locus_tag="WGR04_000629"
     CDS             complement(24416..24853)
                     /locus_tag="WGR04_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0794236.1"
                     /translation="MKISIFWLYNAFILERSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(25264..26562)
                     /locus_tag="WGR04_000630"
     CDS             complement(25264..26562)
                     /locus_tag="WGR04_000630"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ0794237.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMEGWKESTFGIKNYEE
                     LPDNAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(26666..27817)
                     /locus_tag="WGR04_000631"
     CDS             complement(26666..27817)
                     /locus_tag="WGR04_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0794238.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADIGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGAVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            28152..29531
                     /gene="norM"
                     /locus_tag="WGR04_000632"
     CDS             28152..29531
                     /gene="norM"
                     /locus_tag="WGR04_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ0794239.1"
                     /translation="MLLDLNRFSFSVFLKEIRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            29680..31116
                     /locus_tag="WGR04_000633"
     CDS             29680..31116
                     /locus_tag="WGR04_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0794240.1"
                     /translation="MFIKSICLSNFKGFIGDNHQIKFKIPDGATAGSGLNIFIGENNS
                     GKSSIFEAINFLRNGIKEDEAYRIKSKLADDTQPNDACVELAFCGNIQNAITYFVQRN
                     KQAAFNNAINNEQNLKAKRNTVSCKKLDLWDNESQDYSNPSGIDAPFKALFETNFIWA
                     DTNPNDEAAFGSTTLCGLLLKEIAQAHINSAEYQTFRDSFNQVFNSPASNLRQQIATI
                     EQKVQRIFTEQFGQADIHFRFDELKIDSFFKNASIFIDDGVDIPMSEKGNGMQRSVAL
                     ALLQVYAEELAHDEEQGQTKPFYLFIDEPELCLHPKGQTKLLEALLEISRTKQVFLTT
                     HSPYFLVTPHLSNVGLFIFRKDGISNIVEDASLEPMFPWSPTWGEINFKAYKLPTVEL
                     HNELYGKLQHDCGQFREKDFEQWLQSRNIQFTKQWIRENNGTAQAPYDVTLQTFIRNH
                     IHHPENRRNALYTENELRQSIEEMIRLL"
BASE COUNT         7541 a         7883 c         7573 g         8340 t
ORIGIN      
        1 aatccgccgg agtctgaaat ttgatgcacc ttatatattg cgtccctaag aagggatgat
       61 taacaaaaat taacgccctt tactttcttt tccgattggg aacattcccg tcgtacaaac
      121 aggcaaatga aggaacagaa atcacattca aacggcgttc cgccgcctct acaccgcttc
      181 ccagtaatcg atatacagct gcaattcgag attgttgcgc cattcgttgg caacggggcg
      241 gtaaaccgtg cggatgtatt cggggatgtc ttcgctgcaa cgccaaaaca tggcttcaaa
      301 ttcgcagccg tctttttgca gccagacttt tttgtgtttg ccctccgcgc ccaaaggttg
      361 ctggcggacg acgtggaact cgtcggtaaa gctcggcggc gcgaagccct gcccccaaac
      421 gtgacgggca aggttttgcg cctgttccaa cgtgatgtcg caggcgggca ggctgccgtc
      481 ggtgatgaag gtttgcgaca aatcgtcttc gcacaccatt tcgcgcacgg cttcttcaaa
      541 ggacgtctga aacgcgggaa tgttgtgttc aagtatgctc aaacccgccg ccatcgcgtg
      601 tccgccgaat ttcaaaatca aatcgggatg gcgtttggac accaagtcca aagcatcgcg
      661 caggtgcaaa ttgggaatgg aacgtcccga accgcgtact tcgccgttgt cggcaggcgc
      721 aaacacgatg gtcggacgat aaaaacggtc tttgaggcgg ctggcgacaa tgccgaccac
      781 accttgatgg aagtcgtcgc gatacgccac caaagtcgtc tgacctgaag gcagggtttc
      841 aggaaaagca ttcagcgcgt cttgcagcat agactgctcg atttcgcgac gctcgatatt
      901 gaggttgttt aactgagccg ccagttcctg cgcttccaca tcatctcgcg ccaacagaca
      961 ggcaatgccg accgacatat cgtccaaccg tccggcggca ttgatgcgcg gccccaacgc
     1021 aaagcccata tcaaacggct gcgctttgcg ccagtcgcgc cgcgccactt caaacaaggc
     1081 gcggataccg gggcgcattt tgcccgaacg catccgtttc aaaccttgcg acacgaggat
     1141 acggttgttg tgatcgaggg agacgacgtc ggcaacggta ccgagtgcga ccaaatccaa
     1201 aaggtcgccc agattcggct cttttaggcc gtctgaaaaa taattgcggc ggcgcaattc
     1261 ggcacgcaac gccatcaata cataaaaaat cacgcccacg cccgccaagc ttttgcttgg
     1321 aaaaccgcag cctttttggt tcggattgac gatgatgcag tcgggcacgg tctcggcagg
     1381 cagatggtgg tcggtaacga tgacatccaa acccaattcc tgcgccctcg ccacgcctgc
     1441 gatgctggcg ataccgttat caaccgtaat cagcaaatcc acaccttgct ctgccgcgat
     1501 ttcagccagc tcgggcgtta agccgtagcc gtgttcaaag cggttgggca cgaggaaatc
     1561 cactttcgcg cccatcgccg ccaaaccgtc caaaccgacg gcacacgccg tcgcgccgtc
     1621 ggcatcatag tcggcgacaa tcaggatttt ttcctgacgc tcaaccgcat ccgccaaacg
     1681 gcgggcggcg gcttcgcaat tcgtcagcga ttgataaggc aggagggaag cgagtttgtc
     1741 gtccaattcg gcaggacttt gcacaccgcg cgaagcacaa agccgggcga ttaaaggatc
     1801 ggcaccggcg gtgagcaaat gattaaaaac gtcggtattg acggagcggg tttggatttt
     1861 gacggacata acgtggaact ttacagtgcg ggtgtgcttg aaatgccgtc tgaaggtttt
     1921 gcagacggca ttgccgttca tttgtctgag gaattcgcgg ctgcaatcaa cgccgccata
     1981 ggcaagacca gtaccgcacc cgccgcatcc agtatcaacg tggtgggcgt atatgcaata
     2041 ttttcaaaca acttggattt gtcggtatgt tttttcgtaa ccgtgtaata aatatcggca
     2101 ggcacacttt gctcaaaatg ataatcggcg ttcagttttt gcggtgtggc gtagtatttg
     2161 cctttggcgg agacgcagcg cgtgtaaatg gtccgattgt cgagttcgac cgcttcaaac
     2221 tcaagctgtt tcagcttggc gatgtcggca ggtctgtcgg tatcgtagcg caggcaaagg
     2281 tcgtcggtac tgaaattctg gctggcgggc gattcgagtt tgaccggcag ggcttggtag
     2341 gcaaagcgcg ggttgggctc aaccatttga aactgcttgt ccaacccggc cttcaaaatg
     2401 cccgtcagct tcgccgaatc ttcaggattg acgacgaacc agtatttccc gcccatcatc
     2461 accaggctgc ccttttccaa ttgggcattg tctttggcaa ccacaccgaa ggcgcggatt
     2521 tgatccttgt caacgtgttt ataggcggtc gtttggctga acgggtcgtt catgccccac
     2581 atcattgccg tacagccatt cagcatcaag gttgccgcca aaacggcggc ggtcgtttta
     2641 cggaacaaca ttttatcctc caatctaaat aacggcggat gtcgtctgaa aatatgccta
     2701 aatttccgcg tccccgtttt ccataacggt tttcaattct tgaaacaggg cgcggaaatt
     2761 tttcggcggt ttgttttgct cctgctcttt tttggtgttg cggatcaagg tacgcaattt
     2821 accggcgtcc gcatgcggaa aatccgacat aaactgcgtc aacgcgccgt cgtctgccaa
     2881 cagccgtacg cgcgcctgtt ccacacgttg caaaaaggca ttgtgcaccg catcgtcgcc
     2941 gcgcagcttg gcaagaaacg cctcgatggg cgcgggatcc gtgtcgcgca tcagcctgcc
     3001 gataaattgc gcctgccgct tgagcgcgcc gttggacgta atttttttat aggtaacgac
     3061 ggcttcgtac aaatcttcgt ccaagccgat ttttttcagc gtgtcgtttg agagcttggt
     3121 cagttccata cccaaatcct gcaaaccgtt catctgcttt ttcatttggg ttttgctgat
     3181 ccattcgtct tcttgttcaa acatcttgat tctcgaattt tttccaatag cgcattttaa
     3241 cagaatgccg ccgcgttcag acggcatttt gcaccgtccg ccctgcaaac ggcgcgcttt
     3301 tgcccctgcc gcccgaaacg gttaaaatgc cgcaaagcat catcaagaaa gatttctatg
     3361 ctgttcaacc acaccgcttc cgaactcctc gacctctgcc gccgcacgct cgacttggca
     3421 aaagcgacgg gcgcaaccgc cgccgaagcc gatttcagcg aatcattggg acaaagcgtc
     3481 agcgtgcggc tgggcgaaat cgaacaaatc gagtttcagc aggacaagtc gctggacatt
     3541 accgtttacg tcggcaaacg caaaggccgc gccggcaccg ccgatttctc cgaacaagcc
     3601 ctgcgcgaca ccgtccgcgc cgccgccgac atcgcccgcc acaccgccga agacggttgc
     3661 gccggacttg ccgatgcctg tctgatggca aaacacatcg gcgaccccga cctttaccac
     3721 gaatgggatt tggatacgga agccgccgtc gagttggcaa aacaatgcga acaagccgcc
     3781 ctgaatgagg atgagcgcat cgaaaactcc gaaggcgcgg cggtgcaaac cggccattac
     3841 caatacgttt acggcaacac ccacggtttt gccgcacacc ggcaaggcac gcaccacagc
     3901 atttcctgca gcgtcgttgc cgccgacgaa aacgggatgc agcgcgacta ctggtacgat
     3961 tccgcctgcc gccatccgga tatggacagc cccgaaacca tcggtcaaac cgccgcccgc
     4021 cgcactttgc gccgactcgg cagccgcagc attccgactg gaagctaccc cgtcctattc
     4081 gatgcgaccg tttcgggcgg tcttatcgga cacctcgtcg gcgcactctc cggcggcgcg
     4141 ctctaccgcc aaagcagttt cctgatcgac agcatcggca aaaaagtcct gcccgatttc
     4201 ctcaacctgc gcgaagaacc gcacatcccc cgctctttcc gcagcagcta ttttgatgcg
     4261 gaaggcgttg ccaccgcacc gcgcttcgtg attcaaaacg gcattgtcga aggctatttc
     4321 ctcagcagtt acagcgcgcg caaactcgga atgcagacca cgggcaatgc cggaggcgcg
     4381 cacaacctgt atttgaacca tacgcacgaa acgcaatccg acctgctgaa agaaatgggg
     4441 acgggattat tggttaccga actgatggga cagggcgtaa acgccattac cggcgactac
     4501 tcgcgcggtg cggcgggttt ttgggtggaa aacggcatca ttgcctaccc cgtccatgaa
     4561 attaccattg ccggccgcct tcaggatatg taccgcaaca tcgtcggcgt gtccgatgat
     4621 gccttacgcc gttcgtccaa caaaatcggc tcgattctga ttgcggggat gacggttgcc
     4681 ggaagctgaa cgccctgccg atgccgtctg aagacgaagg cggcacgttt tgccccgtgc
     4741 ctgcccgttt gccgttttat gccgcaccgc cttttgccga caacattgca cattttttca
     4801 tcagcactga ttttccctta tccgtcaaaa tactaagcca cttgtccaca acagggtaca
     4861 cacaagttca ggataaggcg tgcgaggatt gggggttttt ccgccgccgg caaaacggca
     4921 gacaaaaacg gcgggacatt gcctgtcccg ccgttttcaa atatacattt gaccgtacat
     4981 caggaattaa gcagcctgaa tgttggcggc ctgtttgcct ttagggccgg tggttacgtc
     5041 gaaagagacg cgttggcctt ctttcagggt tttgaaacct tccatattga ttgctgagaa
     5101 gtgagcgaac aaatcttcgc cgccttcatc aggcgtgatg aaaccaaaac ctttagcgtc
     5161 gttaaaccat tttacgatac cggttgccat tagaaacttc ctatactcaa aaattaacaa
     5221 aatcagcaaa acaaggcata cggcaaaccg agccgtctgg cgttccttcc agcttgatat
     5281 actcggcgga tgcccaatcc tgcttaaccg tctttttaca tttgttaaga caaaacgtca
     5341 agctatgttt tcaaaaaagt gggaaaatag gcaaaagtac cgaaaataca acaaaaatgc
     5401 cgtctgaacg cggctttcaa atgggacaca ctatgaccgc gcaacaccaa tccgacacgc
     5461 tcttgcaccg tctgaacacc ctgccgccga aacgttacgg cgttttccta ttgaacgacg
     5521 attacaccac gatggaattt gtcgtcgaaa tcctgaccga aatctttatg ctcggacaag
     5581 aacaggcggt agcggtgatg ctcttggttc atcacgaagg caaaggcctg tgcggcactt
     5641 acacgcgcga tattgcccaa accaaacaac aacaagtcat gcagcgggca aaagccgaag
     5701 ggcatccgct gcaatgtatt gtcgaggaga tttaatatgc ttgcacccga attggaacag
     5761 attttgcagc agctttaccg cgaggcgcgt aaggctcatt atgaatttat cagcctcgag
     5821 catctgcttt tggtactcat cgaagaagat gccgccgtgc cgaacgtctt aaaactctgc
     5881 ggcgcggatt tgaaagtggt gtccgaacag ctcgccgcca gcgttgccga aaacaccccc
     5941 ctgattcccg aacacctttt agacacggtc gaaacccggc ccacgctcgg cttccaacgc
     6001 gtgatgcaac gggcgatggt gcatacccag tctgccggaa aagccgcggt cgaaccgttg
     6061 gacgttttgg tcgcgctgat gagcgaaacc gacagccaca ccgtctattt cctcaagctg
     6121 caatcggtta cgcgttttga agttttgcgc tgtattgccc acggctctcc cgatgaagat
     6181 gaagatgaag acgatggcaa ctattcttca gacggcatgg acgacgataa tgaaaaccgc
     6241 accaaaccgg gcaaaaaccc tttatcggcg tacaccgtca acctcaacgc cgaagtcaaa
     6301 gccggccgta tcgacccttt gattggtcgc aaacacgaaa tggaacggct ggtgcaaatc
     6361 ctgtgccgcc gccgcaaaaa caatccgctt ttggtcggcg aggctggcgt gggtaaaacc
     6421 gcgctggcgg aagggctggc acatcaaatc gtcaacggcg gcattccaga cgcgcttaaa
     6481 gatgccgaag tgtacgcgct ggatatgggc tcgctgttgg cgggcacgaa ataccgcggc
     6541 gactttgaag cgcgggtcaa atccgtcttg aaacagctcg aaaaaatccc gcaagccatt
     6601 ttgtttatcg acgaaatcca caccatcatc ggcgcgggca gcaccagcgg cggcaccatg
     6661 gacgcgtcca acctgctcaa acccgcgctg gcaaaaggtt cgctgcgctg catcggcgcg
     6721 accacctacg acgaataccg caccattttc gacaaagacc atgccttaag ccgccgcttc
     6781 caaaaaatcg acgtggtcga acccaccgtt tccgaaaccg ttcaaatcct gcgcggcttg
     6841 aaaccgatgt ttgaagcctt ccaccaagtc cgctacactc aaggcgcact cgaagccgcc
     6901 gccgaactct ccgcacgcta catcaacgag cgtttcctgc ccgacaaagc catcgacgta
     6961 atggacgaag caggcgcggc gcaacggatt ctgcccaaat ccaaacagaa aaaagtcatc
     7021 ggcaaagcac aaatcgaaac cgtcatcgcc aaagtttcgc ggattcccga aaaaaccgtg
     7081 tcgcacgacg acaaacaagt gttgcaattc ctcggccgcg atttgaaaaa catggtttac
     7141 ggtcaggaaa acgccatcga cgcgttggtt accgccgtca aaatgtcgcg ctccggcctt
     7201 gccctgcccg acaaaccgat aggcagtttc ctcttctccg gtccgaccgg cgtgggcaaa
     7261 accgaagccg ccaaacagct tgcctactcg ctgggcgtac cgctgcaacg ctttgatatg
     7321 tccgaataca tggaacgcca cgccgtatca cgactcatcg gcgcgccacc gggctacgtc
     7381 ggctttgaac aaggcggcct tttgaccgaa gccgtcaaca aacagccgca ttgcgtattg
     7441 ctcttggacg aaatcgaaaa agcccacccc gacattttca acgtcctcct gcaagtcatg
     7501 gacgcaggca aactgaccga caacaacggc aagagtgccg atttccgcaa cgtcatcctg
     7561 attatgacca ccaacgcggg cgcggaaagc ctcagccgcc ccagcctcgg ctttaccgcc
     7621 aagcgcgaac gcggcgacga aatgcaggca atcaacaagc tcttcacgcc cgagttccgc
     7681 aaccgcttgg acgcgattat cccgtttgca cccttgtccg aacccatcat cgtcaaagtc
     7741 gtggacaaat tcctgctcca gctcgaacac cagctcctcg acaaaaaagt cgaagccgaa
     7801 ttcacgccgg cattgcacaa atatctggcg gaaaaaggtt ttgacccgca aatgggcgcg
     7861 cgcccgatgc accgcctgat tcaggaaaaa atccgcaaac cgctcgccga cgaactgctg
     7921 ttcggcaaac tatccgacgg cggcttcgtg cggatagact gggatgcggc aaaagaagaa
     7981 gccgtgttga agtttaagaa aagcaaggtc aaaataaaaa ccgcctccgc ataaaatcaa
     8041 aaatgccgtc tgaaaggttc agacggcatt ttgtcaaata tcggtcccta ttccggcaaa
     8101 cttctccaca aacgccgcaa tcttttgaaa aacactttgt ttcttcgtca gatattgcgg
     8161 gttgagcgga ctcattttcg gcagggtttc ggtcagttcc gtaccgtttt cgctggcata
     8221 gccgcgtttg agcgagccgg tcaaatagcg tttggcggcg gtttcgttca gaccttcggc
     8281 ggcaatcaat cctgccgctt cgtgccgcat cacctcttgc gcgtaggtgt agaaggtttc
     8341 cagtatggcg ggaacgtcgg ggactttgtc caaatccgta tcgttgatga aatccacaat
     8401 cagaccctct ttggcgcggt gtccgatact tgcgcggatg atgcggcgga tttcttccac
     8461 cagctccgct ttgcctttga tttttttgtg gtgctcgaaa accagttgca ggatgtaatc
     8521 caaattgatt tcctgcgatt tgagcaaatc cacctcaaaa accacatcgt cccagtcgat
     8581 ttttgactgt tctttctgtt cgcctgcttt ttggcggcgc aaccaatcgc ggatgtcgtt
     8641 gtaggcggaa cggtagtcct gcaccgccct ttcagacggc atcggcactt gccgcatttc
     8701 ctgcacgtct tcatcgctca ggtagtattt ttcttgaaac gccttcatcg catcttcgtc
     8761 cgccgcgtcc acactctgca actcgcgcag cgcggcaaat tcatcgtagt tctgcaatac
     8821 gttttccgcc cgcaggtatt cgccgaagag tttggcaaaa tcttttttgt ctttttccgt
     8881 ttcgattttg tcgggttcgg ggaaacgctc gcgcaattct tttgccacat ccaaatagcc
     8941 gcgccgtgct tcgcccgtcc gatcatcgat atagccgttc atgtattctt cgtaactttt
     9001 ttccagcact acatttttgg tgtttttgtc gccaaacaag gtaatcgcat caatggttgc
     9061 ctgctccaaa tcgcggaagc agacaatatt gccgaaggtt ttggtggcat cgtaaatgcg
     9121 gttggtgcgc gaaaacgcct gcatcaggcc gtgatagcgc aggtttttat cgacgaacaa
     9181 cgtgttcagc gtcggcgcgt caaaacccgt caaaaacatg ccgaccacaa tcagcaaatc
     9241 tatttcctga tttttcaccc gttttgccaa atctcggtag tagttttgaa aggctttgct
     9301 gtccgtgccg aaattggttt tgaaacaggc gttgtaatcg ttgatggcag cctgcaaaaa
     9361 ttcttttgcg ctgctgtcca tcgcttccgg ttcaaaagtc tcatcgacaa tttcaccgac
     9421 ggcgttttgc tcttcgttgg ccgcaaagga aaaaatggtg gccactttca gcgggtgcaa
     9481 gctgcctgcc tgttgtgttt tgaacgcttc gtaatagcac ttcgccgcat ccacgctgct
     9541 gacggcaaac atcgcgttaa agcctttgcc acccgcattc agccggtgcg ttttctgcct
     9601 gaactgattc aggatatatt gcgtgatttc gcggatgcgt tcggggtgca gcagggcttg
     9661 gcggttttcg gcggcactca gtttcttctc gtcctgttcc gcttccacag ctttgaactg
     9721 cgggcgcacg tcgttgtaat ccactttgaa tttcaatact ttttcatcgc ggatggcatc
     9781 ggtaatcaca taagaatgca gctcccgccc gaacacgccc gccgtggttt ccgcgcccaa
     9841 agcgttttca ggaaaaatcg gcgtgccggt aaagccgaac tggcagaatt tcttaaattt
     9901 ctttttcagg tttttttgcg cttcgccgaa ttgcgagcgg tggcattcgt cgaaaataaa
     9961 gacaactcgc tgatggtaaa ccggcagatt atcttcactc ttcatcaggt tgttcagctt
    10021 ttggatggtg gtaacgatga ttttgttgtc gtctttttcc aaattgcgtt tcaagcccgc
    10081 cgtactttcc gaaccgttca cactgtcggg cgaaaaacgc tggtattcct tcatcgtctg
    10141 atagtccaaa tccttcctgt ccaccacgaa gaaaaccttg tcgataaacg ccgattccgt
    10201 cgccagacgc gccgccttaa agctggtcag cgttttgccg ctgcccgtgg tatgccaaac
    10261 atagccgccg ctttccggtt tgctccaatt cttcgcctgc gccgaactgt taattttcca
    10321 cagaatgcgt tcggcggcgg caatctggta cggccgcata atcagcagcg tatcattcgc
    10381 atcgaacacg ctgtaatgca gcaaaacgct cagtaatacg cttttttgca ggaacgtggc
    10441 ggtaaagtct ttcaaatcct taatcggatg attgtccgac cgcgcccaat tcatcgtgaa
    10501 atcgaagctg tttttgtcgc gcttggtggt gttggcgaaa tagcgcgtgt ccgtgccgtt
    10561 ggaaatcacg aagatttgca ggaatttgaa cagcgaattt tcgctgttga agctctcttt
    10621 gctgtaacgg tgcacctgat tgaatgcctc gcgcaccgcc acaccgcgct ttttcaattc
    10681 aatctgcacc agcggcaggc cgtttaccaa cacggtaacg tcatagcggt ttgcatgcgt
    10741 gcccgtctgc tcaaactggt tgataagctg cacatggttg cgggcaaggt ttttcttgtc
    10801 cagcagataa atgtttttca gacggccgtc atcaaaagcg aaatcataga tatggtcgtc
    10861 gtggattttg cgggttttat cggtaatgtt ttcagacggc ctgtccagat attccgtcaa
    10921 aaaccgcgcc cattcgccgt ctgaaaacgc cacatcgttc agccgctgca actgcgcgcg
    10981 caggttttcc agcagcctgc tttggctgtt caaatccttg cggtattcgt aaccctgatt
    11041 ctgcaaatcg gcgattaact ccgcttccaa ctggttttcc gattggtagc tgcccgactg
    11101 ttcgattttt tcatattggt cgagcacgat gaaattcggc gtttcggcga tgggtttggt
    11161 ttcgaggttc atatgggtcg tcctttgcgg tgtcgggttt tatagtgaat taaatttaaa
    11221 ccggtaccgc gttggctcgc cttgccgtac tatttgtact gtctgcggct cgccgccttg
    11281 tcctgattta aatttaattc actataaacg gtttgatgtt cggattgggc tgcctgaaaa
    11341 tgtgcaggct gctttttcag gtagccttag ggaaggcaag cagctgttcg cggtaatatt
    11401 cgtattgttt gcggcgcagg gcgatttcgt gcggcaggcc ctcactgatg gagtgggtta
    11461 gggtgtcgaa tttgtctagg atggcgacga ttttttcctg ttcggctaga ggggggatag
    11521 ggattaaaaa gtttacaagg tcttttttat ttaacgcagg aatacctgcc atatttgcaa
    11581 gatttataat cttggacata tcttttgaca ttacatagta taagaactta gtcatgataa
    11641 gattgttatt accccgtatc ctgtaacaaa gtgggccgca ccaaaactta cttgtttgat
    11701 atcctacata tccaattcca ccttgaccac gagatggtgt agttattgta tctggctcgg
    11761 tattatatga agcaatataa cctgatggaa ttgtccctgc attaattaat ggataattac
    11821 caatttcatt tgtttctggt gatttcccaa cattaatatc actaacctcc cccaacgtct
    11881 tccaaaccac atttttcaga cggcctttat agtcatcagc tatccccccg atttgattgt
    11941 caaaatctaa aagaaagtcg cggtaatact ggtattggcg tttgcgcagg gttaattctg
    12001 cttccagcgt agcttccagc gtagcttcca gctcggtgaa tttgtcaagt atttttacaa
    12061 tttcttgctg aatttctaga ggcgggatgg gaatttggat tttccccatc tggtggctca
    12121 ttagttttgg gtttcccata ccagaataaa catgtttttt tgcctcaatt gaaagccaat
    12181 aaaataaaaa tttatagttt aactcaagga tgtttttaat ttttattaat ccacatacat
    12241 ttgtaatcga gaatttccct gaccgataaa atactgttcc cgcatttgca ccatcagttg
    12301 tccaactaac aaattcccca tcaaagtcga atgagtttat ttttccaatt tcaccattat
    12361 ttagagtttg agaactatat acaggataat ttcctaaatt gtcagctaaa taaccctttg
    12421 atataactct tcctctcgtt aagtctgcta cttcagttaa aagcttccac tccaccggtg
    12481 ccgtctgaat catctcaatc aattttttcg ctttgttttg catatccatg gttgttttcc
    12541 gtttctttcc cgctgcggcg ggatgtgggg cggttatcca ccgtaggttg ggcttcagcc
    12601 caccaatccg cctgctgttt tgccgttgtt tcggtgggtt gaagcccacc ctactttgtc
    12661 tgcaaggtgc gcgccgacca atattcattg cctgtttcgt cgatgtgctt ggcgttttca
    12721 aaagcgttgt tttctgttgt catgtatttt tccccgaaaa tttcttgatt tgaacttgta
    12781 aggattgctt acaggttgcc gcccgtattt tcagacggcc tctttcccat tcagttgttg
    12841 caaaatatgc aacaactgcc taggcctcaa tctctgcaat cacttcgtca atttcgtgcc
    12901 gcagccgttc gattttggca acggtttcgc tgatttcggc gttaagctgt ttgatatcga
    12961 taatttcgcg agtgtcttcg gcttcgacat agctgctgac ggcgaggttg tagtcgttgt
    13021 ctttgacagt ttgctgggcg gcgttttggg cgatatgcgg cacatcggct ttatcggcga
    13081 agagtttgac gatttcggca atgtgttctt cggttaagac gttgttgttg gtttctttct
    13141 taaagaagcc gcttgcgtcg atgaattgga tgtcggtatt gtctttatgt ttggacaaaa
    13201 ccagtatatt gacggcgatg ccggtgccgt aaaagagatt gggtgcaagg gcaatcacgg
    13261 tttccacgta gttgccctcc accagatatt ggcggatttt ctgttctgca ccgccgcgat
    13321 agaaaatgcc ggggaatgag acgatggcag cgcggcctct gctagaaagg tagttcagtg
    13381 cgtgcaggat gaaggcaaaa tcggctttag attttggggc gagtacgcct gcgggggcaa
    13441 agcggtcgtc gttgattaag gtggggtcgt cgctgcctat ccagttgatg gaataaggcg
    13501 gattggaaac gacggcatca aaaggtttgc tgtctttgag cttgggattg gtcagtgtgt
    13561 cgcccaattc gatgtggaat tggttgtaat tgacgttgtg caggaacata ttcatgcggg
    13621 cgaggttgta ggtggtgtgg ttgatttcct gcccgaagaa gccttcttcg atgatgtgct
    13681 cgtcaaactg ttttttcgcc tgcaagagca ggctgcccga gccgcaggcg gggtcgtaga
    13741 ttttgttgac tttctcctgc ccgtgcaccg ccagccgcgc aatcagtttg gatacgtttt
    13801 gcggggtgaa aaattcgccg ccggatttgc ctgcgttggc agcgtagttg gaaatcaggt
    13861 attcgtaggc atcgccgaaa aggtcgatgt ggtggtcttc aaaattgccg aaatcgagtt
    13921 cctctacgcc tttgaggacg gcggcaaggc gtttgttctt gtcggcaacg gtgctgccga
    13981 gccggctgct ggtggtgtcg aagtcgtcaa acaggccttt aatgtcttgt tcggacggat
    14041 agccggaggc ggagctttca atcgcggtaa aaatttcttt cagcttggtg ttgagctctt
    14101 cgttttgatg ggcttcggcg gcaatattgc aaaaaagctg gccggggtag atgaaatagc
    14161 ctttgacttt gacggcatcg tctttgattt cgggcgtgat gatgctgtct ggcatagcgg
    14221 cgtaatcaat actgctgtcg cctgcctgca tatagtcggt aaagttttcg ctgataaagc
    14281 ggtagaaaag tgtgccgaga acgtattgtt taaagtccca gccgtccacc gcgccgcgta
    14341 cttcgtcggc aattttccaa atttggcggt gcagttgggc gcgttgttgc atttcggtca
    14401 tcatcgaaat ccatataaaa gttaaacaaa tcgaaatcgc ctgatatttt cagacgattt
    14461 tttacgggca ttcaaattaa gccaaatttt ccagcatcca tttgacgtag cgctcgacgc
    14521 ctgatttgac atcgaaaaat tcttccgtat aacctgcctc acgcaatttg gtgatgtcgg
    14581 cttgagtgaa gctttggtat ttgcctttga gcgcatcggg aaatgggatg tagcggataa
    14641 gttcttcttc taccagctct ttcaagctca tttcgggttt gccttcggcg gcgcggcagg
    14701 cgttgacggt ggcggcggcg agttcgttaa actgttggct gcggccggtg ccgaggttgt
    14761 agatgccgga aagttcggga tggtcgaaga agtagaggtt gactttggca acgtcttcga
    14821 cgctgacaaa gtcgcgggtt tgttcgccgt tgccgtagcc gtcgttgctg ccgaacaggt
    14881 tgacgtaacc gtgttcgcgg tattggtgga aatggtggaa ggcgacggat gccatacggc
    14941 ctttgtgttg ttcgtgttgt ccgtacacgt tgaagtagcg gaagccgacg acttgggcgg
    15001 tcaagccttc tttcatgcgg cggcgcaata cttggtcaaa caggaatttg gaatagccgt
    15061 acacattgag cggtttttcg agttcgcgct cttcacggaa gatttcgcct ttaccataca
    15121 cggcggcgct ggaggcgtag aggaagggaa tgcgttcgtc ctgacaccag tccaacaaat
    15181 ccagcgtgta ctggtagttg ttttccatca tatacaaacc gtcgtgattc atggtgtcgg
    15241 aacacgcgcc ttgatggaaa acggcttcga tgttttgata aggtaaaatg tgttccctca
    15301 cttggcggat gaattcatgt ttgtcgagat aatgggcgat ttcgcactcg gcaaggtttt
    15361 tgaatttttc gcctttgctc aaattatcga cggcaacaat gtcagtaata ccgcgttgat
    15421 taagtgcttt gacgatgttg ctgccgataa agccggccgc gcctgttacg atgatggtca
    15481 tattcggttt cctttgtttt gagtaaaatt aataaactta gttacaagaa atcatcgata
    15541 ttgtcctgaa acacaaattt gcgatgatat tccaaatatt tttctcttcc ttcgggatat
    15601 attctcatct taattttttg catttggatt gatattaagt ttttcccatg tgtaacggct
    15661 tagtcgagta ccgcaaatca aacgaccatc atccaagaaa gtaatataac cttgatcgaa
    15721 caaccaatca taagtcggcg ttaaagctaa cccatttaaa taatctaatg cctctttctc
    15781 atttttctca gtaatacaaa ccatataagg tttaatatga ctagccctta acaaaatctc
    15841 atctgtaatt aatgtaaatg gacattgcgg catatgattt ataacatcct tgcgatattt
    15901 ctgctgccct tttctacttt tctgtttaat gatttcttgc tctacgaatt gattaaattc
    15961 tgaagccggt atttctaatt tttctcttga taagagttgc gggtgcacga tagagcgaaa
    16021 ttgataatcc aaaaatattc ggaaataaaa taatggttct gaatcttcta tatctttgac
    16081 aggcaatagt ttaagaattg ataaataaat aattttgggc aatacaatct tacgccaaat
    16141 tgaccatata tcatcctccg aacggatata acccctacgg taacctttag aattttccaa
    16201 atggtctgat acatcatgga ttgaaaagaa aatattttct gtaggcagat tattaatact
    16261 taaaacgtaa tcatcataaa accttgaaat atcatgatga tatttctgtt cttgtttgtg
    16321 atattcaact tttgcatcaa ccaaataacg ctgtaaattt ttcttattaa aaaaacaatt
    16381 tttctgaatg aaaggatatt ctaccctcct tccatttttt gtctcagtaa tcccccaatt
    16441 ttcatactca aaaaatgtac ttagttttcg accactatca tcaatataac tacctacata
    16501 tttgcgtgct tccccattac caccaaaaac agataacttg ttattcctgt gaataaaact
    16561 atcttccgca cgtaaatttt gaatactatc gacaataaaa tactctatgt tttcaatagt
    16621 aattctttga aaatccggca ttttactctc cataaagcgt aatatccatt tgttttaata
    16681 tagcgattaa tacatcaaca acaatactgt ttcccgcttg gcgatacatt tgagtatcac
    16741 taactaaaat tttaaaatca tctctaaaac ccattagccg caaacattcc ctaggtgtta
    16801 atttacgaat acgtccacga tcatgagtaa cataattatc aaccccggca cgatgcattt
    16861 tatgcatagt ttgcaataat gggcgagcta caggcaaatc agtttccgta ctggttttaa
    16921 aattttttgt tcctcctgcc aaaacataat ttttgatttt ttcagaaaga taatattttt
    16981 cctcaacgtt atttacatca aaaataaagt catcaaattc agattcgcgg gctgcctgaa
    17041 aaataaaatc accatgccaa ttaaattgtt gatttgcttt ttggcataag gcaatttctc
    17101 cattaatctg tgtataacgt ttttgtctat ttttagaact ggtaacaaat ttcgcaccct
    17161 tttcacgtaa aaaatatttg ctatcagtat agtcctccaa aaaatcttgc atagtatgtt
    17221 ctaattcaat cttttcagga aactgaaaac catttatagg aggggtatga aagccaacaa
    17281 caaaaatacg ctcacgatgt tgaggaatcc cataatcctt actattcatt atttggaaat
    17341 ataagtcata accaagtgaa taaaaaacac tttttacaac tttccaagtt tttccattat
    17401 catgattgag caagcccttt acattttcat aaataaatat ctttggttgg acttcatcca
    17461 caacgcgagc aaattcatag aataatgttc ctcgtgtatc ttctaatcct gcacgtttgc
    17521 caaccatgga aaatgcttgg caaggactgc ctccaactaa aatatcaact tgatttctaa
    17581 actttctcgc atcaaattga gtaatgtcgt tatgccaaaa atcttcattt aatttatagt
    17641 ttccaagata acttttttaa cgtatggatc aatatctcct gcaaaaacaa tggtatggtt
    17701 taaatttaat cggtgaaatg cctgttcaac cgcaccaatc ccgctgaata cggttgctaa
    17761 tctaatatgt gaatcaggtt taagaaaagt tttagattgc caaccttgtt gactgggaaa
    17821 gagcaaagtt ttttgtaatc gagtatcgtg tgtctgtgcc attgtcgaaa tagtcatact
    17881 tatatcgttc tgtttatctt atcaatatga aaactacatt gttgattgcc ctgacaatgc
    17941 cttggtcaat tcggcaaacg agcaaaccgc cgtaccgagt ttcgccacga caaccccggc
    18001 cgcagtattg gcaaggtaca tggcttcggg catggtgtag cctgccgcca gccccaaacc
    18061 cactccggca ataacggtgt cgcccgcacc ggatacgtcg taaacttctt gggcgcgggt
    18121 gggctggtaa atcggttcgc cttcactgaa caaggtcatg ccttcttcgc ttcgggtcag
    18181 caaaatcgcg gtcaaatcga ggtggcggcg caggttttgc gctttttcgg tcagctcgcc
    18241 ttcgtttttc caactgccga ccacttcttt caattcggcg cggttgggcg taatcagagt
    18301 tgcgccgaca tatttttcgt aatcgtcgcc tttggggtcg attaatacgg ttttgccttc
    18361 gtgtttcgcc caatcgatca tatcggagat gtgcgacagg ccgcctttgc cgtagtctga
    18421 aaaaatgatt gcgtcgtatt cgggcaagat ttcgcggtat ttccgcttga tttgttccaa
    18481 cacttcgcgg ttgggatgtt cttcaaaatc aaggcggata agctgctggt tgcgggcgac
    18541 gacgcgcagt ttgacggtgg tggcgatttg tttgtcgcgc atcagatagg aggcaacgcc
    18601 gtcctgcacc atcagcgcgt cgagcgcgtc ggcggcttcg tcgtcgccgg ttaccgacaa
    18661 cagccctgcc ctgccgccca acgaagcgat gttgcgcgca acgttcgccg caccgcccgc
    18721 gcgttggtcg atacgtccga tttttgccac cggcacgggg gcttcgggtg aaatacggga
    18781 cacgtcgcca aaccaatagc ggtcgagcat cacgtcgcca acaaccagaa ctttggcttg
    18841 tgcgaaacgg gatttgaggg tttcttgttg aaacttggcg ggcatattgc ctccgttgtg
    18901 ttttgttcag acgaccttaa ccggtaagac cgcctgaaaa tcagtttgct tcaaggtttg
    18961 ccacgcggtt cacttcgcgc aatacggcta gcggatcggc agcttgggta acgggacggc
    19021 ccatcacgag ataagtcgaa cctgccgcca aggcttcggc gggcgtcatg atgcggcgtt
    19081 ggtcgtcgtt gttgccggca acgtccaagc ggataccggg cgtgaccaag acaaaatctt
    19141 gtcccaattc gcggcgcagc ggtgcggctt cttgggcgga gcagaccacg ccgtccaagc
    19201 ccgaactttg cgccagtttc gccaaacgga tgacttgttc ttcgggagcg atgttcaaac
    19261 cgatttccgc caaatcgctt tgttccatgc tggtcaacac ggttacgccg attaagagcg
    19321 gctttgtgcc gtatccggca acggcttctg ctgcggcttc catcatacgg cggccgcccg
    19381 acgcgtgcat atcgaccatc caaacgccca tgtcggcagc gactttgcag gcttgcgcga
    19441 cggtgtgggg aatatcgtgg tatttcaaat cgaggaaaag tttgaaacct tgattgatta
    19501 agctttctgc caaactgcgt cccgtcgcgg taaacagctc tttgccgatt ttgatttgac
    19561 acaatgtcgg gtcaaggttg cggacgaatc cgagcgtgtc tttttcgttg gcaaaatcaa
    19621 gggcgacgat gacgggggtg cgttgttgcg gagtttggaa gtcggagatt aaaggattca
    19681 tggtgttttc gtttcgggtt ttcagacgac ctttatcgtc tttttcgcag tatagtggat
    19741 taactttaaa ccggtacggc gttgtctcgc cttagttcaa agagaacgct tctctaaggt
    19801 gctgaagcac aaagtgaatc ggttccgtac tatctgtact gcctgcggct tcgtcgcctt
    19861 gtcctgattt ttgttaatcc actataacaa cgtaaattca aaccgtaggt cgggcattga
    19921 tgtccgacct acgcggactc aagcctccga caaactgttt cggataaatt tgaaccggta
    19981 catcagccgt cattcccgcg caggcgggaa tccattgata aaactcagtt catcagattt
    20041 aaacagcgat tgcccaaatt cagaaatgga ttcccgcctg cgcgggaatg acgccaaatg
    20101 attatttttc ggttgcaaca cgctgtttat caaaaacagg tcgtctgaaa acctgatttt
    20161 ctgttttcag agtgcgaagc ctgcccgtgg gtacgacctt ttcgatttat ttgcccaatg
    20221 cgcccaatac ttcggctttc acggcttcta ccggctgagt gccgtcaact ttgatgtatt
    20281 taggcgcgtg ttcgccttcc agtttgctgt aaaaatcgac caaaacttcg gtttgctcgt
    20341 ggtaaacggc aaggcgtttt ttcacggttt cttctttgtc gtcgtcgcgc tgaatcaaat
    20401 cttcgccggt tacgtcgtct ttgccttcaa ctttgggcgg gttgtaggta acgtggtaag
    20461 tacggccgga agccaaatgc acgcggcggc cgctcatacg gtcgacaatc acgctgtcag
    20521 gcacgtcgat ttcaacgact gcatccaaat ccacgcctgc ttcaaccatc gcttcggctt
    20581 gtgccaatgt gcgcgggaaa ccgtcgaaca ggaaaccgtt tttgcagtcg tcttgcgcga
    20641 tgcgttcttt gaccatgccg ataatgatgt cgtcgcgcac caagccgcct tcgtcaatga
    20701 tttttttcgc ttccaaaccc aagggcgtgc cagccttaat cgcggcgcgg agcatgtcgc
    20761 cggtagagat ttgcggaatg ccgaacgcgg cggtgatgaa ttgcgcctga gtgcctttgc
    20821 ccgcgcccgg cgcgcctaaa agtaatgctt tcatgaagtg atcctttgaa atgtgtttat
    20881 tggaatcaaa cagatggttt acgattctac acggcttggc acaatagttc aaaaaccaac
    20941 tgcacaaact gccatttgca agcaaatgtt ttgcatacct ttgccgagtc tgatattcac
    21001 aagccggcaa ctttttggga caatccccaa aatttcaaaa catcacaccc taccaaaaag
    21061 gaacatccgt gaaacgaatt tttctgtttt tggctaccaa tatcgctgtt ttggtcgtaa
    21121 tcaacattgt tttggcggtt ttgggcatca acagccgggg cggcgcgggc agcctgttgg
    21181 cgtattccgc cgtcgtcggc ttcactggtt cgattatttc gctgctgatg tccaaattca
    21241 tcgccaaaca atcggtcggc gcggaagtta tcgacacgcc gcgcaccgaa gaagaagcct
    21301 ggcttttgaa cactgtcgaa gcccaagcgc ggcaatggaa cctgaaaaca cccgaggtcg
    21361 ccatctacca ctcccccgaa cccaatgcct ttgccacggg cgcatcgaga aacagctccc
    21421 tgatcgccgt cagcaccggt ttgctcgacc atatgacgcg cgacgaagtg gaagccgtat
    21481 tggcgcacga aatggcacac gtcggcaacg gcgatatggt tacgctgacg ctgattcaag
    21541 gcgtggtcaa tacctttgtc gtgttcctgt cgcgcattat tgccaacctg attgcccgaa
    21601 acaacgacgg cagccagtcc cagggaactt atttcctggt cagcatggta ttccaaatcc
    21661 tgttcggctt ccttgccagc ttaattgtca tgtggttcag ccgacaacgc gaataccgcg
    21721 ccgatgcggg cgcggcaaaa ctggtcggcg cgccgaaaat gatttccgcc ctgcaaaggc
    21781 tcaaaggcaa cccggtcgat ttgcccgaag aaatgaacgc aatgggcatc gccggagata
    21841 cgcgcgactc cctgctcagc acccaccctt cgctggacaa ccgaatcgcc cgcctcaaat
    21901 cgctttaaac cgatttgaaa cggcaaaaac cgtacgcgca agcagtacgg ttttttgtat
    21961 atgctgtctg aaaacatcag gtttccatac cgacaccaac cgtatccatt tccgcaccgg
    22021 atacgccgca ttgatatacc ctgataaacc gtatccggac agaaaatata gtggattaac
    22081 aaaaaccagt acggcgttgc ctcgccttag ctcaaagaaa acgattctct aaggtgctga
    22141 agcaccaagt gaatcggttc cgtactattt atactgtctg cggcttcgtc gccttgtcct
    22201 gatttttgtt aatcccctat atcagataaa acagcggtaa aattgccgtc tgaaaccaac
    22261 tctgcgccat acgggacacg ctctgcacgt ctatttgcct ccgatatatc ctccctactg
    22321 taggaacgtc tgccgatttg cacgaaatcg gcacctctag cacccttcag gatggcggac
    22381 gttatccgtt cttgggattc tgcaagacgg tcgacggcat tatacagacg ctcggtctga
    22441 tacccttccg aagcaacctg cttcaggtat tccaaataac cgtaacccaa cgcccctccg
    22501 acaagcaggg tcgtcaacgc taccagcgtt gccgaacgga ttttaggggg catatcttga
    22561 agcatatctc taccgaaccg aaccaaagtc tctttaaagt cggactctac acgcgtagaa
    22621 ccctcggaaa tcttgaagtt caactcatac tgcttccgtt tgtccgctgt caaccgcttg
    22681 aggttgtcat ccccgtattg gacggttgac acaaccctgt acagactgtc ctgtatttcg
    22741 accagcgtct gtgcaaagtt ggcggtcata actggattcc caacccttgc cgccgattct
    22801 gatactcaaa acggtatcca ggcgttccag cagggcttcg ggagacagca gtgcgtattc
    22861 ttcaatattt tccataggtt gattatagca gaaccttata cctgttacag acaaggttgt
    22921 cgggatttga acgtccgtcg agcttggttc gtatagagcc gtagggggtg tttagggctt
    22981 tggcggcagc ttgtacgctg gggaacgtga gtgtttcgcc tgtggcggtg tcgaggatgg
    23041 agacaggttt ttggttttgg ggaggcgtat tttccttcat tttaagtttc ccgcactccg
    23101 gacacccata ctttgacttt atagctgtgt cgtaatacaa aagttggaca accccgtgcg
    23161 taggacatct aacactggaa ggtttataaa tgccactgaa ctccaacaat tctaaatgtg
    23221 gaaatttctc ttgaaagcgt gatttggcgg tatcgaaagt cattcgctta cccataattt
    23281 gtctccatag gttatgtgca aatggattac acaaccaaaa tatagaaatt ttcaaatttt
    23341 ttgagaccta cctgaaatcc tgcgcaagca caatgtaaag aaaaatggta tacgtcttta
    23401 ctaaatcttt acataaatta actctaaccg ggatttgaac gtccgtcgag cttggttcgt
    23461 aggatttagg gtgttgtgaa ccttacctat cgtacccaag cactttgtac ctgtttgaaa
    23521 taaggttgtc gggcgacgtt cgacccttga gcctgtgatt gatttgttgg aaatgtacac
    23581 ctaaagcagc acctgcggca gtaacgcttg ggaacgtgag tgtttcgttg gtcgttgtgt
    23641 cgaggatgag gaggggcttc cctacatttg gaggtgtttg ggtttttgac atcttagcac
    23701 actcaggaca cccgtacttt gactttataa gggcattaaa tgtcgaataa cgaacgttcc
    23761 cgtgcatagg acaattaacg gttactgggt aattcacacc gtcaaagccg gttagagaga
    23821 tattagggaa tatatcagta agttcagaag ctgcttggtt gaaagatttg gccatttgag
    23881 actccaaaat tgattgtcaa atggatttaa tagcaaattt ataaaaatgt caaatttttc
    23941 gagaagcacc tgatagtggc gaggggtata cactgttaaa aagtgtatac gtcatttagt
    24001 gcagatgtga accttatacc tccctgcaag taaagtctct gtgtgaaccc gaccttttat
    24061 acggtcagtg atagctgctg ggtttatacc caatgacctt gcagcagatg ctcttgaggg
    24121 gaatgtaagt gtttcgccag ttacagtatc ctccaatact gtatttttag ataatttatc
    24181 tattgtatct tctggaattt tgtgggaatt gacaccataa gatgcacatt ttggacaccc
    24241 acttttagag tccaacatac ttttgaatgt agagcaagta accctcccgt gtataggaca
    24301 gacgattacc gtcgggtaac gaacgccatt gaactccaca agattcaaat gtggaaattt
    24361 agaggttagc ttagaaactg cttgagtaaa tgtaagtgcc atagtggttg tgaacttact
    24421 ttaccaacct ataacgatac ctaatcaatc ggtcaaacgg agtgcgtccg cttaaacgta
    24481 cagctaaagt gctgttggag caaccgataa atttactggc agccagtgta cttgcaaact
    24541 cgtagatttc ccctgtctct gtgtcctcca acctaatggc aacggcgttt ggagagggct
    24601 tttcgggatt tcgagtacat tcaggacatc ctgttgagga tttcatggca gcttgtagat
    24661 tcccgtaaac aaccgaccca tgcaaagggc attggattgt taccggttcg tagtttttag
    24721 tgaacttcaa aaccgtctta tcaggaaatt tactggaaaa ccttaactga acatcttgta
    24781 gcgagagtga ttttggcatt atagaccttt ctaaaatgaa agcattatac aaccaaaata
    24841 tagaaatttt caaattttct ctgaacgcac tggtatccga tggagctaaa tgtaaagaaa
    24901 agggtatatg tccttactaa atctttacac attttaactg tatagtgttt gacaaacaac
    24961 aggcataggc gcataatgca cccgtagtta gatagatagt aaggagataa ccaatgactt
    25021 agcacctgcc cccctctctc tcgcgcaccc cgcccctcgc ttgcggctaa tgggctttgc
    25081 gcgaaatata cactttcatc tcctgcatgg cttaaccgcc tatgcaatag tacacacatc
    25141 aaccaatcag gctggcacgt cttggcattg gctgacgtac cattgtagcc cgaaatgttg
    25201 ataccctact atttatgacc aacggcggat tagggcttca gacggcaatt ttactgcaaa
    25261 accttatgcg aacggatgat gcagcacaat cgtttcttcg cggtcggggc cggtggagac
    25321 gatggcgacc ggcgcgccgc agacttcttc aatccgtttc aaatatgctt tggcattatc
    25381 gggcagttcc tcataatttt tgatgccgaa agtcgattct ttccaaccct ccattgtttc
    25441 gtaaatcggt ttgcaggttt ccaccgcatc cgaaccgcaa ggcaggatat cggttttgcc
    25501 gccgtcgggc aattcgtagc cgacgcagat attgatggtt tcaacgccgt ccattacatc
    25561 gagtttggta atgcacatac cggaaatgcc gttgatttgg atggagcgtt tcagggcggc
    25621 ggcatcaaac cagccgcagc ggcgtgcgcg gccggttacc gagccgaatt cgtgtccgcg
    25681 ttccgccaaa cctgcgccta cttcgtcgaa caattcggtc gggaacgggc ctgaaccgac
    25741 gcgcgtggta taggctttga cgatgcccaa aacataatcc agcatttgag gacctacgcc
    25801 cgcgcctgcg gaagcggctc ccgccagaca gttggacgag gtaacgaagg gataagtgcc
    25861 gtagtcgatg tccaacaacg caccttgcgc gccttcaaac agcagttttt cgccgttttt
    25921 gtttttctcg ttcaacacgc gggacacgtc ggtaatcatc ggcgcaatgc gcggcgcgac
    25981 tttttcgata accgccatca cgtcttccgc tttaaccggc tcggcattgt gcagatgttg
    26041 cagttggaca ttgtaatagg caaggacggc atccagtttt tcacgcagtt tttcaggatg
    26101 cagcaaatcg gcggcgcgaa tggcgcggcg tgccactttg tcttcgtagg cagggccgat
    26161 gccgcggccg gtcgtgccga ttttgccttt gccgcgcgat gcttcgcggg cttggtcgag
    26221 cgcgatgtga taaggcagga tcagcgggca ggtcggcgcg attttcagac ggccttcgac
    26281 gtttttcacg cctgccgcgt tcaactcgtc gatttcgccc aacagggctt cgggggagac
    26341 gacaacgccc gaaccgatga agcagtccaa actttcatgc aggatgccgc tcggaatcag
    26401 gcgcaaaatg gtttttttgc cgccgacaac caaggtatgg cccgcattgt ggccgccttg
    26461 gaagcgcacc acgccgccgg cttcttccgc cagccagtca acgattttac ctttaccctc
    26521 gtcgccccac tgtgcgccga ttactacaac atttttagcc atagccatat aacctatcga
    26581 tattaaaaat tattgcggaa atccccaaac cggtgcggct ttgcgccccg ccacgatttg
    26641 cctttcatcc gccatgaacg ggtatttagc gtttcacgac ctgccaaacg ccgtccgtct
    26701 ttttcagacg gcctgcaagc tcttccgaaa cattgtgtcc gataccgtaa tcgattacga
    26761 cacactgccc ttgttcacgc aaggcttcga ccgcttcgcg cgccgcttcg gcatcttccg
    26821 catcgaccaa cacggcgggc tgccgttcga tggcgggcaa acgcccgata aagctgcgca
    26881 agtcgaaact gaatcccgtt gccgggcgcg ccctgccgaa atatccgccc aatccgtcat
    26941 aacgcccgcc gcgcgcgacc gcgtcgtgga aatcggcggc ataggcggca tacagcaagc
    27001 ccgtgtggta attgtcgaca cgcagctcgg acaagtcgat atggatttca caatcgggga
    27061 atgcgtcgca caccgcctgc aattcgccca acgcgccgcc gaccgccgac aaatccggca
    27121 accgtccgcg cgcgtcggac aacacttcac gcccgccgta caggcgcggc aacagcgaga
    27181 atgcttttgc ccacatgccg tccagcttcc aagccttgac ctgcgcttcg accgccccgg
    27241 tatctttatc ctgcatcaag gcaagcagcg ttgcggactg ccccgcatcc aaatgcgccg
    27301 catcggacaa ggcgcggaat atgccgatat gccccagcga aagcagcact ttgcctatat
    27361 cggcaatttt catgcttttc agcatcaggt ctatcagctc gatgtcgcca cggatgtcgg
    27421 caaaaccgta catttccgcc cctgcctgca agggttcgcg catattcagc agaccgtcgg
    27481 gctgcgcgtg caacaccgga ccggcataac acaaccggtt aatcccttgg ttggcggata
    27541 aaagatgggc atcaatacgc gccacctgcg gcgtgatgtc ggcgcgtatg cccaactgcc
    27601 tgccgctgag cctgtccgtt accaaaatgg ttttcaggga aagccccgca tcgatatgcg
    27661 tcagcaggga atgtgcgtac tccatcagcg gaggctgtac cagttcataa ccgtgtacgc
    27721 ggaacagtgc caacaactgc tccctcgcgc tttcaagctg ccgcgcgttc gtgggcagta
    27781 cgtcggcgat atgttccgga agctgccatg tctgcatgag aatctgcctt ctattttatc
    27841 atgtaaatac aaaaggctgc ccgatccgca atcagacggc atctttgccg caacgcatca
    27901 cgccgctgtc aaaacggcga tgcttccttc cgattcagaa tccgaacagc ccctgtccga
    27961 cggctcaatg ccgtcaagtc gttaaaacca aactttacca taaaatacac acaatctgaa
    28021 tatactttta taccctgtat ccgccgtctg acggcacggc atttttttac tatataatgt
    28081 gcgccattat ttctcatcac ttcctgctgc cgttttctgc ttcagacggc atttttgttg
    28141 attgaacact tatgctgctc gacctcaacc gcttttcctt ttccgtcttc ctgaaagaaa
    28201 tccgcctgct gaccgccctt gccctgccca tgctgttggc gcaggtcgcg caggtgggca
    28261 tcggttttgt cgataccgtg atggcgggcg gtgcgggcaa ggaagacttg gcggcggtgg
    28321 ctttgggcag cagcgcgttt gccacggttt atattacctt tatgggcatt atggcggcgc
    28381 tgaacccgat gattgcccag ctttacggcg cgggtaaaac cgacgaagtg ggcgaaacgg
    28441 gacggcaggg gatttggttc gggctgtttt tgggcgtgtt cggcatggtc ttgatgtggg
    28501 cggcgattac gccgttccgc aactggctga ccttgagcga ttatgtggaa ggcacaatgg
    28561 cgcagtatat gctgttcacc agcttggcga tgccggcggc aatggtacac cgcgcactgc
    28621 acgcctacgc ctccagcctg aaccgcccgc gcctgattat gttggtcagc tttgcggcgt
    28681 ttgtgttgaa cgtgccgctg aactatattt tcgtttacgg caaattcggt atgcccgctt
    28741 tgggcggcgc aggctgcgga ctggcgacga tggcggtgtt ttggttcagc gcgctggcat
    28801 tgtggattta tatcgccaag gaaaatttct tccgcccatt cggactgacg gcgaaattcg
    28861 gcaaaccgga ttgggcggtg ttcaaacaga tttggaaaat cggcgcaccc atcgggctgt
    28921 cttatttttt ggaagccagc gcgttttcgt ttatcgtgtt tttgattgcg cctttcggcg
    28981 aggattatgt ggcggcgcag caggtcggca tcagtttgtc ggggattctc tatatgattc
    29041 cgcaaagcgt cggctcggcg gggacggtgc gcatcggctt ttcgcttggg cggcgcgaat
    29101 tttcgcgggc gcgttatatt tcgggcgtgt cactggtgtt aggatggatg ctcgccgtga
    29161 ttaccgtgct ttccttggta ttattccgtt cgccgctggt aagtatgtac aacaatgatc
    29221 cggcggtttt aagcatcgcc gccaccgtct tactgttcgc cggcttgttc caaccggcag
    29281 acttcaccca atgtatcgcc tcctacgcct tgcgcggcta caaagttaca aaggtgccga
    29341 tgttcatcca cgccgccgcc ttttggggct gcggtctgct gccgggctac ctgctcgcct
    29401 accgtttcga tatgggcatt tacggcttct ggacggcatt gattgcctcg ctcaccatcg
    29461 ccgccatcgc cttggtgtgg tgcttggaat tgtgcagtag ggagatggtc agatcgcata
    29521 aggctgtctg aaaactccat gggtaggatg aagtctgcta attacaatat taatagatga
    29581 tgtggcaggg ttgagctagg agaatttaga gtttttgaag ggagagagtt tggtgtttta
    29641 gcttaatctc tatttctaac ttaagataag gaaaccgtta tgtttataaa aagtatttgt
    29701 ttatctaatt ttaaagggtt tatcggtgat aaccatcaga ttaaatttaa aattcctgat
    29761 ggagcaactg ctggtagtgg tttgaacatt tttatcggag aaaataactc tgggaaatcg
    29821 tctatttttg aagctataaa tttcttaagg aacggtatca aagaagatga agcttataga
    29881 ataaaaagta aactggcaga tgatactcag ccaaatgacg catgcgttga gctggctttt
    29941 tgtgggaata ttcaaaatgc aataacttat tttgttcaaa ggaataaaca agcagctttt
    30001 aataatgcaa ttaataatga gcagaattta aaagctaaga gaaatacagt ttcttgtaag
    30061 aaattggatt tgtgggataa cgaatctcag gactattcaa atccaagtgg gatcgatgct
    30121 ccttttaagg ctctttttga aactaatttt atttgggcag atacaaatcc taatgatgaa
    30181 gcggctttcg ggtctacaac tctttgcgga ttgttactga aagaaattgc tcaggcgcat
    30241 atcaattcgg ctgaatatca aacatttcgt gatagtttta atcaggtatt taattcacca
    30301 gcttctaatt tgcgtcaaca aattgctact atcgaacaga aagttcaacg tatttttact
    30361 gaacaattcg gacaagctga tattcatttt agatttgatg aactaaagat agattcgttt
    30421 tttaaaaatg cctctatctt tattgacgat ggtgtggata ttccaatgtc cgaaaaaggt
    30481 aatggtatgc agcgttctgt tgcattagcc ttgcttcaag tctatgcaga agagttagcg
    30541 catgatgaag agcaaggtca gactaagccg ttttatttgt ttattgatga gcctgagctt
    30601 tgtttacatc caaaagggca aactaaatta cttgaggcgt tgttagaaat ttcaagaacg
    30661 aaacaagtat ttttgactac gcattcccca tattttttgg ttacacctca tttaagtaat
    30721 gttggtctgt ttatttttag aaaggacgga attagcaata ttgttgaaga cgcttcattg
    30781 gagccaatgt ttccttggag cccaacttgg ggagagatta atttcaaggc gtataaatta
    30841 cctaccgtgg agttacataa tgaattatat gggaaattac aacatgattg tgggcaattt
    30901 cgagaaaaag actttgaaca atggttacag agtaggaata ttcagttcac taaacaatgg
    30961 atacgagaaa acaatggaac cgcacaagct ccatatgacg taaccttgca aacatttatc
    31021 cgcaaccata ttcatcatcc tgaaaatcgg cgaaatgcac tttatacgga aaatgaactt
    31081 agacaatcta ttgaggaaat gattagattg ttatagttga aggagtaaaa agtgcggtca
    31141 attctctcaa tgttttcaaa acgtcgtctg aagccacact ttctagccca aagaagaaaa
    31201 ttcagttatt caaaattttt gggtaaccgt tgatgatggt aaaaactaaa aaactttgat
    31261 cgcctgctga aataaggaca atttgaggat aaacagcaat aaacctgttt aatctttgtt
    31321 ttgataaaaa catatgg
//
LOCUS       DAWFJR010000016        29869 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWFJR010000016 DAWFJR010000000
VERSION     DAWFJR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29869
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.016"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            195..551
                     /locus_tag="WGR04_000634"
     CDS             195..551
                     /locus_tag="WGR04_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ0794241.1"
                     /translation="MLSAYFPVFVFILIGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFIYEWKKGALEWE"
     gene            542..1024
                     /locus_tag="WGR04_000635"
     CDS             542..1024
                     /locus_tag="WGR04_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ0794242.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            1037..1630
                     /locus_tag="WGR04_000636"
     CDS             1037..1630
                     /locus_tag="WGR04_000636"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ0794243.1"
                     /translation="MASIQNLYETVSRVLGDRAGKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFEFLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDN
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            1620..2876
                     /gene="nuoD"
                     /locus_tag="WGR04_000637"
     CDS             1620..2876
                     /gene="nuoD"
                     /locus_tag="WGR04_000637"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ0794244.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELEGETIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKKQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            2876..3349
                     /gene="nuoE"
                     /locus_tag="WGR04_000638"
     CDS             2876..3349
                     /gene="nuoE"
                     /locus_tag="WGR04_000638"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ0794245.1"
                     /translation="MLSAESLKQIDTELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQR
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            3739..5040
                     /gene="nuoF"
                     /locus_tag="WGR04_000639"
     CDS             3739..5040
                     /gene="nuoF"
                     /locus_tag="WGR04_000639"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ0794246.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            5040..5477
                     /locus_tag="WGR04_000640"
     CDS             5040..5477
                     /locus_tag="WGR04_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794247.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            5721..6032
                     /locus_tag="WGR04_000641"
     CDS             5721..6032
                     /locus_tag="WGR04_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0794248.1"
                     /translation="MRNHPLTRQPAVYILANGKNGTLYIGVTMNLPERVWQHKNHVNI
                     DGFTARYDVHDLVWYQFFENMPEAVAKEKTMKKWRREWKIKLIEEQNPEWLDLSGVLF
                     V"
     gene            6314..8575
                     /locus_tag="WGR04_000642"
     CDS             6314..8575
                     /locus_tag="WGR04_000642"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ0794249.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIIQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAN
                     GLGVKNFATRLRQQDKRLSDDLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELLMPLLSQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELAAATGAVLGILPQAANSVGADVLNVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLSSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDAIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVMHLPLHTENAA
                     LGALMDTIELAGA"
     gene            8578..9654
                     /gene="nuoH"
                     /locus_tag="WGR04_000643"
     CDS             8578..9654
                     /gene="nuoH"
                     /locus_tag="WGR04_000643"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ0794250.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGIVGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            9735..10214
                     /gene="nuoI"
                     /locus_tag="WGR04_000644"
     CDS             9735..10214
                     /gene="nuoI"
                     /locus_tag="WGR04_000644"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ0794251.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            10245..10952
                     /locus_tag="WGR04_000645"
     CDS             10245..10952
                     /locus_tag="WGR04_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794252.1"
                     /translation="MEGLINALKYLAEHEPIDNFEEIRIRNSPIELPSGLSNFEQNIF
                     LKENLSPKLQNDDSLKTHYWIIREWGGIKSFKQSAENSQLIRQFLSELNSGKLSSGLL
                     KISSLSKLASFIDCERFAIYDSRAIFSLNWLLFKFTNADLFFQPQGRNRELEIRNMNV
                     LFHFSDIKPNYRKPDVSFHQYCGLLQDLAKQVYGKQAKPYHIEMLLFKIATTWICADM
                     DQLIKFDCLRNQDFQTA"
     gene            10988..11659
                     /locus_tag="WGR04_000646"
     CDS             10988..11659
                     /locus_tag="WGR04_000646"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ0794253.1"
                     /translation="MTFQLILFYIFAVIILYGALKTVTAKNPVHAALHLVLTFCVSAM
                     IWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTNLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTINPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKPE
                     GEGKA"
     gene            11656..11961
                     /gene="nuoK"
                     /locus_tag="WGR04_000647"
     CDS             11656..11961
                     /gene="nuoK"
                     /locus_tag="WGR04_000647"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ0794254.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDGLKG"
     gene            11995..12570
                     /locus_tag="WGR04_000648"
     CDS             11995..12570
                     /locus_tag="WGR04_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine monophosphate-protein transferase
                     NmFic"
                     /protein_id="HEZ0794255.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            12694..13035
                     /locus_tag="WGR04_000649"
     CDS             12694..13035
                     /locus_tag="WGR04_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794256.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVFPK"
     gene            13084..15108
                     /gene="nuoL"
                     /locus_tag="WGR04_000650"
     CDS             13084..15108
                     /gene="nuoL"
                     /locus_tag="WGR04_000650"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ0794257.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFIDGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHSDGHGEEHHGLGKNDNPHESPLVVTLPLILLAVPSVIIGYIAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQAFRPVYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSARLVGAVAAQVRKVQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            15164..15484
                     /locus_tag="WGR04_000651"
     CDS             15164..15484
                     /locus_tag="WGR04_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEZ0794258.1"
                     /translation="MSNTQTIRSADFTTSRAWGALDIANMNGTTVRLHWTDQPYKWHV
                     NDGEEVFAVMDGEVDMHYRENGEEHIVRLKSGDIFYAGIGAEHVAHPRGETRILVIEK
                     EGSV"
     gene            15559..17274
                     /locus_tag="WGR04_000652"
     CDS             15559..17274
                     /locus_tag="WGR04_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEZ0794259.1"
                     /translation="MANLNVSSKKIINLLGNQDMQEKYFIIPEYQRPYRWDTEKCAVL
                     WSDIVDFRQSSTGKNEEYFLGTIVVCPNEKGNLEVIDGQQRLTSLLLLLRAFYRVLEQ
                     TESTPDTAAKITTLKSKIAPCIWDVDVLTAEITDRTAIRLHTQVATETDNEILHEILA
                     NGEISEECRSNYAVNYLYFLKQCQEYARTNPMDWYHLCLTILNDCILLPIESDSLNSA
                     LTIFSTLNDRGLPLSDADIFKARLYKMAQQKDKFVEQWKELVALAEGASISVDDLFRY
                     YTHVLRAKEKITDKEIGLRRFYTDNRNKQLENENLFNHLIELAGFWQDINNSKTNQDV
                     ETSNFVNDEAAKWLQCLNVYPNEFWKYAASVFFIANKGAQSFKDDFAALLKQLTAFLY
                     AKFLVAPTVTAIKDDIYKLCVAIYHGEKNALSYVMPAPIHYEQNTGRITKGLLMINAY
                     LFDEKQKLLPTKTEIEHIFPKKWQETNYNGWSIIDAEQYLEQLGNKIVFEKRLNIQAG
                     NGYFGKKKEQYQKSDIVEVRELAGENANDWLPSDIQKRQQQIVDRLKQFFDNCFEAAS
                     IPKKN"
     gene            17311..18807
                     /locus_tag="WGR04_000653"
     CDS             17311..18807
                     /locus_tag="WGR04_000653"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ0794260.1"
                     /translation="MFSNHLLSLAIWIPIAAGVLVLATGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFVILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVNGQLNDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVILGAIHNPHVAEM
                     KDINCREFAILAILAVAVLGMGLYPQAFIEVVHQAANDLIAHVVQSKI"
     gene            18817..20262
                     /gene="nuoN"
                     /locus_tag="WGR04_000654"
     CDS             18817..20262
                     /gene="nuoN"
                     /locus_tag="WGR04_000654"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ0794261.1"
                     /translation="MNWSDLNLMPAMPEIVLLALLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAAVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALMFTLFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKVAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARPAVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            20374..20673
                     /locus_tag="WGR04_000655"
     CDS             20374..20673
                     /locus_tag="WGR04_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ0794262.1"
                     /translation="MTASMYILLLLALIFANAPFLTTRLFGIVPLKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(20782..21678)
                     /locus_tag="WGR04_000656"
     CDS             complement(20782..21678)
                     /locus_tag="WGR04_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ0794263.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIQYDEATALLVGDALQTQAFDVLARPSGLPASRQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVRLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(21662..21886)
                     /locus_tag="WGR04_000657"
     CDS             complement(21662..21886)
                     /locus_tag="WGR04_000657"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ0794264.1"
                     /translation="MKKNAPKSFEEALSRLESLTQAMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDVDGLKELNLESDE"
     gene            complement(22150..23073)
                     /gene="rsgA"
                     /locus_tag="WGR04_000658"
     CDS             complement(22150..23073)
                     /gene="rsgA"
                     /locus_tag="WGR04_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ0794265.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETRAASPERLAFLQGITDELPG"
     gene            complement(23078..24934)
                     /locus_tag="WGR04_000659"
     CDS             complement(23078..24934)
                     /locus_tag="WGR04_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0794266.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAY
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VNDGMATLSKPHTILDKPRALPLNVPQGAIKFEHIDFCYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGITQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            complement(24960..25097)
                     /locus_tag="WGR04_000660"
     CDS             complement(24960..25097)
                     /locus_tag="WGR04_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794267.1"
                     /translation="MPPEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            complement(25097..25681)
                     /gene="ruvA"
                     /locus_tag="WGR04_000661"
     CDS             complement(25097..25681)
                     /gene="ruvA"
                     /locus_tag="WGR04_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ0794268.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            complement(25763..26089)
                     /locus_tag="WGR04_000662"
     CDS             complement(25763..26089)
                     /locus_tag="WGR04_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0794269.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHNKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(26143..26862)
                     /locus_tag="WGR04_000663"
     CDS             complement(26143..26862)
                     /locus_tag="WGR04_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ0794270.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTTSADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGQSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAVSSSGTNLSVEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            complement(26859..26981)
                     /locus_tag="WGR04_000664"
     CDS             complement(26859..26981)
                     /locus_tag="WGR04_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ0794271.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            complement(26992..27456)
                     /gene="trmL"
                     /locus_tag="WGR04_000665"
     CDS             complement(26992..27456)
                     /gene="trmL"
                     /locus_tag="WGR04_000665"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ0794272.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVYENFDDCLKALAGRRIFALTTKGTAHPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(27521..28018)
                     /locus_tag="WGR04_000666"
     CDS             complement(27521..28018)
                     /locus_tag="WGR04_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ0794273.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLAD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWRILPRHTVFRHHRPPQSTLKG
                     SERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELAKTLKQSDANRICCWTLAR
                     TPMKK"
     gene            28074..28988
                     /gene="bioH"
                     /locus_tag="WGR04_000667"
     CDS             28074..28988
                     /gene="bioH"
                     /locus_tag="WGR04_000667"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ0794274.1"
                     /translation="MCLKHRGQLFAASVRYAAKSAAQPAQMPSETPDEPWHNTQRLIV
                     GASAMRRQQERKFMPDAAKKVYLIHGWGANRHVFDDLMPRLPATWPVSAVDLPGHGDA
                     PFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARL
                     TADEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTHDADGIIGRILPDLARC
                     GTPSALQEALDAAERADARHLLDKIDVPVLLVFGSKDAITPPRMGEYLHRHLKGSRLV
                     VMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            28990..29778
                     /locus_tag="WGR04_000668"
     CDS             28990..29778
                     /locus_tag="WGR04_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ0794275.1"
                     /translation="MNPQDKCWQVHRHLAEYADQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGGFWQRFAGKGVVQHCQSPTAPLPEACAD
                     MLWSNLGLLAAESIVPVLQNWARALKTDGLLFFTCFGRDTLAELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMAWNDENAA
                     RSCVGTIFEREGGLGITLETVYGHAVKKLMLPQGENVVQFFPKR"
BASE COUNT         6845 a         7297 c         8169 g         7558 t
ORIGIN      
        1 atccactata ccacgaatta cggtgtaaaa atttatatga ccttataaaa tcaaataaga
       61 atcgttatca taacatgatt gtatttattg ggtttttttg ggcgttttgc cgatatttac
      121 cttttaatgg tttttgaaat tcgctaaaat acgaaattat tgtagaaatt ttgttaacgg
      181 atttgggtgt aaccatgttg tccgcttact ttcccgtctt tgtctttatc ctcatcggcc
      241 tcgcggccgg cgtactgttt atcctgctcg gcacgatttt aggcccgaaa cgccactacg
      301 ccgaaaaaga cgcgccttac gaatgcggtt tcgaagcctt tgaaaatgca aggatgaagt
      361 tcgacgtgcg ctattacctc gtcgccatcc tcttcatcct ttttgatttg gaggtcgcgt
      421 tcatgttgcc gtgggcggtt gtgttcaaag atttgggcgc gtacggcttc tggtctatgc
      481 tggtgtttat cgtcgttctg acggtaggct ttatttacga atggaaaaaa ggtgcgctgg
      541 aatgggaata gaaggcgttt tgaaaaaagg tttcatcacc accagcgcgg atacggtgtt
      601 gaactatatg cgtaccggtt cgttgtggcc ggttactttc ggcttggcct gctgcgccgt
      661 ggaaatgatg cacgcgggta tggcgcgtta tgaccttgac cgtttcggca tcattttccg
      721 tccgtcccca cgtcaggccg acctgatgat tgtggcaggt acgctgacca acaaaatggc
      781 gccagccctg cgccgtgtat acgaccagct cgccgagcca cgctgggtat tgtctatggg
      841 ctcgtgtgcc aacggcggcg gttattatca ctattcttat tccgttgtgc gcggtgccga
      901 ccgcgtcgtg ccggtagacg tttatgtgcc gggttgtccg ccgactgcgg aagccctgat
      961 ttacggcctg attcagctcc aacaaaaaat caagcgcact tccaccatcg cgcgggacga
     1021 gtagggggag gacgatatgg caagcattca aaacttatac gaaaccgtca gccgcgtttt
     1081 aggcgatcgg gcaggcaaag tcatttcagc tttgggtgag attaccgtcg agtgtctgcc
     1141 cgaacactat atttcagtca tgaccgcatt gcgcgaccat gaagagttgc atttcgagtt
     1201 tctggtcgat ttgtgcggtg tcgattacag cacttacaaa aacgaagtat ggcagggcaa
     1261 acgcttcgcc gtcgtcagcc agcttctttc cgttaaaaac aatcagcgca tccgcgtgcg
     1321 cgtctgggtt tcagacgaca acttccccgt agtcgaatcc gtggtcgata tttacaacag
     1381 cgcggattgg tacgagcgcg aagccttcga tatgtacggc attatgttca acaaccaccc
     1441 cgacttgcgc cgcatcctga ccgattacgg ctttgtcgga catccgttcc gcaaagactt
     1501 cccgatttcc ggctatgtag aaatgcgtta cgacgaagag caaaaacgcg tgatttacca
     1561 acccgttacc attgagccgc gcgagatcac gccgcgtatc gtccgtgagg agaactacgg
     1621 tggccaataa attaagaaac tacaccatca acttcggccc gcaacaccct gcggcgcacg
     1681 gcgtattgcg catgattttg gagttggagg gcgaaaccat cgtccgtgcc gacccgcata
     1741 tcggcctctt gcaccgcggt actgaaaaac tggctgaaac caaaacctat ttgcaagccc
     1801 tgccctatat ggatcgcttg gactacgttt ccatgatggt caacgagcag gcgtattgtt
     1861 tggcagtaga aaaacttgcc ggaattgacg tgcctatccg cgcccaatac atccgcgtca
     1921 tgtttgccga agtaacgcgc atcctcaatc acttgatggg catcggttcg catgccttcg
     1981 acatcggcgc gatgaccgcc attctttacg ccttccgcga ccgcgaagag ctgatggact
     2041 tgtacgaagc cgtttccggc gcgcgtatgc acgcagccta cttccgtccc ggcggcgttt
     2101 accgtgacct gcccgacttc atgcccaaat acgagagcag caaattccgc aatgccaaag
     2161 tattgaagca gctcaacgaa tcccgcgaag gcacgatgct cgactttatc gatgctttct
     2221 gcgaacgttt cccgaaaaac atcgacacac tcgaaaccct cctgaccgac aaccgtattt
     2281 ggaaacagcg taccgtcggc atcggcgtcg tctcccccga acgtgccatg caaaaaggct
     2341 ttaccggcgt gatgttgcgc ggttcgggtg tggaatggga cgtgcgtaag aaacagcctt
     2401 acgaagtgta cgacaaaatg gatttcgaca tccctgtcgg cgtgaacggc gactgctacg
     2461 accgttacct ctgtcgtatg gaagaaatgc gccaatccgt acgcatcatc aaacaatgct
     2521 ccgagtggtt gcgtgtcaat ccgggtccgg tcattaccac aaaccacaaa ttcgctccgc
     2581 ccaaacgtac cgaaatgaaa acaggtatgg aagacctgat tcaccatttc aaactattta
     2641 ccgagggtat gcacgttccc gagggcgaga cctacactgc tgtcgaacac ccgaaaggcg
     2701 agttcggcgt ttacatcatt tcagacggcg caaacaaacc ctaccgcctg aaaatccgcg
     2761 cacccggctt cgcccacctg caaggtatgg acgaaatggc aaaaggccac atgctcgccg
     2821 acgtcgttgc catcatcggt acgcaggaca tcgtattcgg ggaggttgac cgataatgtt
     2881 atccgcagag tccttaaaac aaatcgacac agaactggcg aaatatcccg ccgaccaacg
     2941 ccgttccgcc atcatgggtg cgttgcgtat cgcccaaacc gaaaaaggct ggctcgcccc
     3001 cgaaaccatc gcttttgttg ccgactatat cggcatcacg cctgcacaag cctacgaagt
     3061 cgctaccttc tacaatatgt acgatcttga gcctgtcggc aaatacaaac tgaccgtttg
     3121 taccaacctg ccctgcgccc tgcgcggcgg tatggcgacc ggcgaatacc tcaaacaaag
     3181 actcggtatc ggctacggcg aaaccacgcc cgacggcaaa tttacccttg tcgaaggcga
     3241 atgcatgggc gcgtgcggtg atgcccccgt tatgctggtc aacaaccaca gcatgtgcag
     3301 ctttatgact gaagaggcga ttgagaagaa actggcggag ttggagtagg tcgtctgaaa
     3361 cgacgattta aatgtaggtc ggatacttgt atccgacaga gtgggtaaaa aggcaaaatg
     3421 tcggatttaa gaatccgccc tactgaaata ccgaaatgcc gtcattcccg cgcaggcggg
     3481 aatccaccgg taagattcgg tttctgaatt taataagaca ttgtttacca ttgaggatgg
     3541 attcccgcct gcgcgggaat gacgacagac aagcaagtgg tcgagatcca acaaaaacga
     3601 ttaaaggtcg tctgaaaata tcgatttgat aaactagatt ttatttcaga cgacgttaca
     3661 agccgataca caaagacatc ttaaggtcgt ctgaaacagc ggccgcaacc gatacgaaaa
     3721 caaacaggca caccaaaaat ggctatttac caatcaggcg tgatttttga ccaagtggat
     3781 accgccaatc ccgattgctg gacattggac gaatacgtca aacgcggcgg ctataccgcc
     3841 ctgcgtaaaa ttctgtccga aaacatctcg caaaccgatg tgattgacga agtcaaaacc
     3901 tccggcttgc gcggtcgtgg cggtgcgggc ttcccgaccg gtttgaaatg gagctttatg
     3961 ccccgttctt tcccgggcga aaaatatgtg gtttgcaaca ccgacgaagg cgaaccaggt
     4021 acgtttaaag accgcgacat catcatgttc aatccgcatg ccctgattga aggtatgatt
     4081 attgccggtt acgcgatggg cgcgaaagcg ggctacaact acattcacgg cgaaattttt
     4141 gaaggctacc aacgctttga ggccgctttg gagcaggcgc gtgccgcagg ctttttgggt
     4201 aaaaatattt tgggttcgga ttttgaattc gaactcttcg cccaccacgg ctacggcgca
     4261 tacatctgcg gcgaagaaac cgcattgctc gaatcgttgg aaggcaaaaa aggccagccg
     4321 cgctttaagc cgccattccc tgcttcgttc ggcctgtacg gcaaaccgac taccatcaac
     4381 aatactgaaa cgttctcctc cgttccattc attatccgtg acggtggaca ggcatttgcc
     4441 gataaaggta ttccgaatgc aggcggtacc aaattattct gtatttccgg ccatgtcgag
     4501 cgtccgggca actatgaagt gccattgggc acaccgtttg ccgaagtctt gaaaatggca
     4561 ggcggtatgc gcggcggcaa aaaactcaag gccgttattc ccggcggttc gtccgcgcct
     4621 gtattgcctg ccgatatcat gatgcagacc aatatggact atgactcgat ttctaaagca
     4681 ggctcgatgt tgggttccgg cgcgattatc gtcatggacg aagacgtgtg catggtgaaa
     4741 gcccttgagc gtctgagcta cttctactat gacgagtctt gcggtcaatg tacgccttgt
     4801 cgagaaggta cgggctggct ctaccgcatc gtccaccgca tcgtagaagg caaaggcaaa
     4861 atggaagatt tggatttgct ggattccgtc ggcaatcaaa tggcaggccg caccatctgc
     4921 gcccttgccg atgccgccgt tttccccgtc cgcagcttta ccaagcattt ccgtgatgag
     4981 tttgtgcatt acatcgaaca cggcggaccg atgaaagagc ataagtgggg agggtggtaa
     5041 tggtggaagc taaaattttt attctatacg gtgcagccaa caaaggtaag agtacgacac
     5101 tcaatacgct ttttaatcag atttgtcgga aattttctaa atttctagtc ttttttgaaa
     5161 gacatggaaa cggcttagat tttgttgcag tatttgatca tgaaggtcag aggattggtt
     5221 tttattcatc tggtgataat gaatacgagg ttaggagaaa tttatacaaa ctttattcgc
     5281 ataattgtga ttttattttt ggcacgtcaa ggacacgggg tggtagttgc gatgcagtag
     5341 gatgttatgc agagttattg catggcgatg taaatataat taattggtgt gaaaagtttg
     5401 agcctacaga tgaagacaat gagcgtgctg ttaaagagtt atttaagtca tttaaaaata
     5461 taataaatga gttatagttt tagttggttt tatattggtt aaaagcaaaa tgctaaaaat
     5521 ttaactttgc cgtcattccc gcgtaggcgg gaatccatag tggaatctaa agaaaccgat
     5581 atttgaaaag cagttgccga aaacccaaga atggattccc gcctacgcgg gaatgacggc
     5641 gggagtaggc agatgttttc agatgaaaac ggttgtaaat gatattaaaa aagttgttgt
     5701 ttatattgca ggaaaaatga atacgaaacc atccgcttac tagacaacct gccgtatata
     5761 ttttggcaaa cggtaaaaat ggaacactct atatcggtgt taccatgaat ttgccggaaa
     5821 gggtttggca gcacaaaaac catgtcaata ttgatggctt tactgctcga tatgatgtgc
     5881 atgatttagt ttggtatcag ttttttgaga atatgcctga agcagttgcc aaagaaaaaa
     5941 cgatgaaaaa atggcgacgt gaatggaaga ttaaactgat tgaagaacaa aatcctgaat
     6001 ggttggactt gtcgggcgtg ttgtttgttt agttttattt ctggaacttt aaaaactgtc
     6061 gttattccag ccccacctac gcgcagacag gctacggcgg gaatcaccgc aaaagttaag
     6121 aaaccaatgt ttgaaaacag ttaccgaaaa cccaagaatg gattcccgcc tacgcgggaa
     6181 tgacggcaag gtggcagtaa acgttttaaa cagtattgat tgtcaatgaa actcaaaagg
     6241 ccgtctgaaa cccattcttc agacgacctc cataaaagat tatttatcaa atacccgtaa
     6301 ctaggaacga accatgttac aaatcgaaat cgacggcaaa caagtatctg tggagcaggg
     6361 cgcgacggtg attgaagccg cgcacaagct cggtacttat attccgcatt tctgttacca
     6421 caaaaaactt tccatcgccg ccaactgccg tatgtgtctg gtgaacgtag aaaaagcccc
     6481 aaaacccctg cctgcctgtg ccacgccggt tacagacggc atgattgtgc gtacgcattc
     6541 ggcaaaagcc cgagaggcgc aggaaggcgt gatggagttc ctgctcatca accatccgct
     6601 tgattgtccg acctgcgacc aaggcgggga atgccagttg caggatttgg cggtgggcta
     6661 cggaaaaacc accagccgct acaccgaaga aaaacgttcc gtcgtcggca aagacatggg
     6721 gcctttggtt tccgccgagg aaatgagccg ctgtatccac tgcacccgct gcgttcgttt
     6781 cactgaagaa atcgccggtt tgcaagaaat tgcgatggtg aatcgcggcg aacactccga
     6841 aatcatgccc tttatcggca aaacggtgga aaccgagctg tcgggcaacg tcatcgattt
     6901 gtgtcctgtc ggcgcattga ccagcaaacc gttccgcttc aacgcgcgta cttgggaatt
     6961 gaaccgccgc aaatccgttt ccgcccatga cgctttgggc agcaacctga ttatacaaac
     7021 caaagaccat accgtccgcc gcgtgttgcc gctggaaaac gaagcgatta acgaatgttg
     7081 gctgtctgac cgcgaccgtt tcgcctacga aggcctgtat cacgaaagcc gtctgaaaaa
     7141 cccgaaaatc aaacagggcg gcgagtggat ggacgtggat tggaaaaccg cgttggaata
     7201 tgtccgcagc gcgattgaat gtatcgccaa agacggcaac caaaaccaag tcggcatttg
     7261 ggccaatccg atgaatacgg ttgaagagct gtatctggcc aaaaagcttg ccaatggttt
     7321 gggtgttaaa aactttgcca cccgtttgcg ccaacaagac aaacgtcttt cagacgacct
     7381 taaaggtgca caatggttgg gacaaagcat tgaatctttg gctgacaacg atgccgtatt
     7441 ggtagtcggt gcgaacttgc gcaaagaaca gccgctcctg accgcccgcc tgcgccgcgc
     7501 cgccaaagac cgtatggcct tgagcgtatt ggccggcagc aaggaagagt tgttgatgcc
     7561 gcttctctct caagaagccg cacatcccga cgagtgggca ggccgtctga aaaacctgtc
     7621 tgccaatgcg gaacacgccg ttaccgccag cctgaaaaat gctgaaaaag cagcggtgat
     7681 tttgggcgcg gaagtgcaaa accatcctga ttacgccgcc atttatgctg ccgcgcaaga
     7741 gctggcagcc gcgaccggcg cagtgctggg cattttgccg caagctgcca acagcgttgg
     7801 tgcggatgtc ttaaatgtaa actctggtga gagcgttgcc gaaatggcaa atgcgccgaa
     7861 acaggcagtc ttgctgctca acgtcgagcc tgaaatcgat acggttgacg gtgcaaaagc
     7921 tgtagccgca ttgaaacagg cgaaaagcgt gatggcattt acgccgtttg tcagtgaaac
     7981 gctgctggac gtgtgcgacg tattgttgcc gattgcgccg tttaccgaaa catcaggcag
     8041 cttcatcaat atggaaggcc gtctgcaatc tttccacggc gtggtacaag gcttcggcga
     8101 ctcgcgtccg atgtggaaag tgttgcgcgt attgggcaat ctgtttgacc tgaaaggttt
     8161 tgaataccac gataccgctg cgattttgaa agacgcgctg gatgcagaaa gcctgtcgtc
     8221 caaactggac aaccgcagta catggacagg ggagggcgtt cagacggcat cagaccgcct
     8281 cgtccgtgtc ggcggcgtcg gtatttatca caccgatgcc atcgtacgcc gttccgcacc
     8341 gttgcaagaa accagccatg ccgccgtgcc tgccgcgcgt gtaaatccga acacattggc
     8401 acgcttgggc ctgcaagacg gacaaaccgc tgtcgccaaa caaaacggcg caagcgtatc
     8461 ggttgctgtc aaagccgatg ccggtttgcc tgaaaacgtg atgcatctgc cgctgcatac
     8521 cgaaaatgcc gcgctgggtg cgttgatgga cactattgaa ctggcgggag cttgatcatg
     8581 caagaatggt tccaaaacct ctttgccgca acgctcggtc tgggcgattt gggcatcacc
     8641 gtaggcttgg tggtatccgt catcgtcaaa atcgtgatta tcctgattcc gctgattctg
     8701 accgttgcct acctgacgta tttcgaacgt aaagtcatcg gcttcatgca gcttcgcgtc
     8761 ggtccgaacg taaccggccc atggggtctg attcagccgt ttgccgacgt gttcaaactc
     8821 ttgtttaaag aggtaacccg tccgaagctg tcaaacaaag ccctgttcta catcggcccg
     8881 attatgtcgc ttgcaccgtc ttttgcggca tgggcagtga ttccgttcaa cgaagaatgg
     8941 gtgctgacca atatcaatat cggccttttg tacattctga tgattacctc gctgtcggtt
     9001 tacggcgtga tcatcgcggg ctgggcttcc aactccaaat attcgttctt gggtgcaatg
     9061 cgtgcttccg cacaaagcat ttcctacgag attgccatga gtgccgcgct ggtgtgcgtc
     9121 gtgatggtgt cgggcagcat gaacttctcc gacatcgttg ccgcgcaggc aaaaggtatc
     9181 gcgggcggtt ccgtattctc gtggaactgg ctgccgcttt tccccatctt catcgtctat
     9241 ctgatttccg ccgttgccga aaccaaccgc gcgccgtttg acgtggcaga gggtgagtct
     9301 gaaatcgttg ccggccacca cgtcgaatac tccggcttcg cattcgcgct gttcttcctt
     9361 gccgaataca ttttcatgat tctgattgcc gcgctgacat cgctgatgtt cctcggcggc
     9421 tggttgtctc cgttcccgca aagctggggc attgtcggta cgccttccgc attctggatg
     9481 tttgtgaaaa tggcggcagt gttgtactgg tacctgtgga tccgtgcaac cttcccgcgc
     9541 taccgttacg accaaatcat gcgtttgggc tggaaagtgc tgattccgat cggcttcgcc
     9601 tacatcgtgg ttttgggcgt gtggatgatt tcaccgctga atttgtggaa ataagtttca
     9661 gacggcatct tgaggccgtc tgaacaaagc gattttgaat acctaacgaa atccctgttt
     9721 tgagggaaca taatatggct aacttagtaa aaactttctt gctcggcgaa ttggtaaaag
     9781 gtatgggcgt aacgctcaaa aacttttttg cccgcaaaga cacaatttat ttccccgaag
     9841 agaaaacgcc gcaatccgtg cgtttccgtg gcctgcacgc gcagcggcgg tatccgaacg
     9901 gcgaagagcg ctgtatcgcg tgtaaattgt gtgaagccgt ttgccccgca atggcgatta
     9961 acattgaatc ggaagaacgc gaagacggca cccgccgcac caagcgttac gatatcgacc
    10021 tgaccaagtg catcttctgc ggtttctgcg aagaggcctg cccgaccgat gcgattgtgg
    10081 aaacgcatat ttttgaatac cacggcgaga aaaaaggcga cttgcacatg accaagccga
    10141 tcctcttggc aatcggcgac aaatacgaag ccgaaatcgc caaacgcaaa gccgctgacg
    10201 cgccgtaccg ttaatgcttt tgggcttctt ggaaggtttt aaatatggaa ggactgatta
    10261 atgcattgaa atatttagcc gaacatgagc caatagataa ttttgaagaa attagaatta
    10321 gaaatagtcc gattgagttg ccaagtggat taagtaattt tgaacaaaat atttttttaa
    10381 aagaaaattt atccccaaaa ttacaaaatg atgatagctt aaagactcat tattggatta
    10441 tccgtgaatg gggtgggatt aaaagtttta aacaatctgc tgaaaatagc cagcttattc
    10501 gtcaattttt atcggaactt aattcgggaa aattgagtag tggtttgttg aaaatttcat
    10561 cattatctaa attggcttct tttatagatt gtgagcgatt cgccatttat gattcacgcg
    10621 ctattttttc gttgaattgg ttgttgttta aatttacaaa tgcagatttg ttttttcagc
    10681 cacaaggtag aaatagggaa ctagaaatcc gaaatatgaa cgtattgttt catttttctg
    10741 atatcaaacc gaattatcgg aaaccagacg tttcgtttca tcaatattgt ggattgttac
    10801 aagatttggc gaaacaagtt tatggtaaac aagcaaaacc gtatcacata gaaatgttgt
    10861 tattcaaaat tgcgacaacg tggatttgtg cggatatgga tcaactgatt aagtttgatt
    10921 gtttgcgtaa ccaggatttt cagactgctt gaaaccatat ttttgattaa taaagaaagc
    10981 atagactatg actttccaac tgattttatt ttatattttt gcagtgataa ttctttatgg
    11041 cgcgctcaaa accgttaccg ccaaaaaccc cgtgcatgcc gctttgcatc tggtgctgac
    11101 cttctgcgtg agcgcgatga tttggatgct gatgcaggcg gagtttttgg gcgttacgct
    11161 ggtggtggtt tatgtcggcg ccgtgatggt gttgttcctg ttcgtcgtga tgatgttgaa
    11221 catcgacatt gaagaaatgc gtgccggttt ctggcggcac gcgcctgttg ccggtgtggt
    11281 cggcacattg ttggcggttg ctctgatcct gattctggtc aatccgaaaa ccaacctggc
    11341 cgcatttggt ctgatgaaag acattccggc cgattacaac aatatccgcg atttgggcag
    11401 ccgtatttat accgactatc tgttgccgtt tgaattggcg gcggtattgc tgttgttggg
    11461 tatggtggcg gcgattgcgc tggttcaccg taaaaccatc aatccgaaac gcatggatcc
    11521 tgccgaccag gttaaagtac gcgccgacca aggccgtatg cgtctggtga aaatggaagc
    11581 ggtcaaaccg caagtcgaat ctgccgaaga aagcgaagtt tcagacggtc tcaagccgga
    11641 aggggagggc aaagcatgat taccttgacg cattatctgg tattgggcgc gctcctgttc
    11701 ggtatcagcg cgatgggcat ctttatgaac cgcaaaaacg tgctggtctt gctgatgtcc
    11761 atcgagctga tgcttttggc ggtgaacttc aactttatcg ccttctcgca acatttgggc
    11821 gatactgccg gacaaatttt cgtattcttc gtattgaccg ttgccgctgc cgaatccgcc
    11881 atcggtttgg cgattatggt attggtgtac cgcaaccgac aaacgattaa tgttgccgat
    11941 ttggatgggt tgaaagggta aaggtaggtt gggtcgagac ctgacaagac accgatgccg
    12001 tctgaaaacc cgataggaaa aacgatgaaa tccatagacg aacaaagcct gcataatgcc
    12061 cgccgcctgt ttgaaagcgg cgacatcgac cgtatcgaag tcggtaccac cgcgggcctg
    12121 caacagattc accgttacct gttcggcggc ttatatgatt ttgcgggtca aatcagggaa
    12181 gacaacattt ccaaaggcgg ttttcgtttt gccaacgcca tgtatttaaa agaggctttg
    12241 gttaaaatcg agcagatgcc cgagcggact tttgaagaaa tcatcgccaa atatgttgaa
    12301 atgaacattg cccatccgtt tttggagggt aatggcagaa gtacccgcat ctggctggat
    12361 ttggtgctga agaaaaacct gaaaaaagtc gtgaactggc aaaatgtaag caaaaccctg
    12421 tatttgcagg cgatggaacg cagccccgtc aacgatttag aactgcgctt tttgttaaag
    12481 gacaacctga ccgacgatgt ggacaaccgt gaaatcatct ttaaaggtat cgagcagtca
    12541 tattattacg aagggtatga aaaaggctga aggtcgtctg aaaagcgatt tcagattgtt
    12601 tcagacgacc tgatttggta ggtgatcaga cgggagcgga tgagaaaaga aattctgggt
    12661 aagaataatc cggtctgaaa tattggaaga agaatgatgg ataaaaatca gttagaacaa
    12721 gaatttcata aagccatgtt aaatatttat caggaggctt tgaatttgcc gcaaccttac
    12781 aaggcgacac gatttttaca aattgtaaat gaatttggtg gtaaagaggc ggcggataaa
    12841 ttattgagta cgggggaaaa gaagactcag accggtttta cagagctgat tttgagtggt
    12901 ggcggagtcc acgccttgaa atacagtatg gaatatctgg tgttacaaaa gccgtggtgt
    12961 gatttattta ctgaagagca attagctgtg gcacgcaaac gattggagcg tgttggattt
    13021 gtttttccga agtaattttg tacgaaacaa acatagattt ttaaatcaat cggattcaat
    13081 caaatgaacg acatgacttt atatctcatc attgccctcg tgcctttggc aggctcgctg
    13141 attgcgggtt tgttcggcaa caaaattggt cgtgccggtg cgcatacggt tacgatactc
    13201 ggcgtggctg tgtccgccgt gctgtcggct tatgtgctgt ggggctttat cgacggcagc
    13261 cgcgccaagt ttgacgagaa tgtctatacc tggctgacga tgggcggttt ggatttctcc
    13321 gtcggcttct tggtcgatac gatgacggcg atgatgatgg tcgtggtcac cggcgtatcg
    13381 ctgatggtgc atatctatac catcggttat atgcacgatg agaaagtcgg ctaccaacgc
    13441 ttcttcagct acatttcttt gtttactttc agtatgttga tgctgattat gagcaacaac
    13501 ttcatccagc tcttcttcgg ctgggaagct gtgggcttgg tgtcgtatct cttgatcggt
    13561 ttctatttca aacgtccgag cgcaacattt gccaacctga aagccttttt gatcaaccgt
    13621 gtcggcgact tcggcttttt gctcggtatc ggcttggtgc ttgcttattt tggtggcagc
    13681 ctgcgctatc aggacgtatt cgcttatctg cccaacgtcc aaaatgccac catccagctc
    13741 ttccccggtg tggaatggtc tttgattacc gtaacctgtt tgctcttgtt tgtgggtgcg
    13801 atgggtaaat cggcacaatt cccgctgcac gtctggctgc ctgattcgat ggaaggcccg
    13861 accccgattt ctgcattgat tcacgccgca accatggtta ccgccggtct gttcatggta
    13921 tcgcgtatgt cgccgattta tgagatgagc agcactgcgc tgtctgtcat catggtaatc
    13981 ggtgcgatta ccgccctgtt tatgggtttc ttgggcgtga ttcaaaacga catcaagcgc
    14041 gtggttgcgt attccaccct gtcgcaactg ggctatatga ccgtggcttt gggcgcgtcc
    14101 gcctattccg tggcgatgtt ccatgtgatg acccacgctt ttttcaaagc cttgttgttc
    14161 ttggcggcag gcagcgcgat tatcggtatg caccatgacc aagacatgcg ccatatgggc
    14221 aacctgaaaa aatatatgcc gattacttgg ctgaccatgc tgatcggcaa tttgtcgctg
    14281 atcggtacgc cgttcttctc cggcttctat tctaaagatt cgattatcga agcagcgaaa
    14341 tacagcacac tgccgggcag cggctttgct tattttgccg tcctcgccag cgtgtttgtt
    14401 accgcgtttt acgcgttccg ccaatacttt atggtgttcc acggcgaaga gaaatggcgc
    14461 agcctgcccg aacaccattc agacggccac ggcgaagaac atcacggttt gggtaaaaac
    14521 gacaatccgc acgaaagccc gttggtggtt accctgcctt tgattttgct tgccgttccg
    14581 tccgtcatca tcggctacat cgccatcgaa cccatgcttt acggcgattt cttcaaagac
    14641 gtgatttttg ttaatgccga tgcgcatccg accatgcaca ttatgaaaga agagttccac
    14701 ggcgcattgg cgatggtgtc gcacagcctg cattcgcccg ttctctatct cgctatcgca
    14761 ggcgtattga gcgcatggct tttgtacgtc aaactgccgc acctgcctgc gaaaatcgcg
    14821 caggcgttcc gtccggttta cgttttgttt gaaaacaaat actaccttga tgccctgtat
    14881 ttcaacgttt tcgccaaagg cacacgcgca ttgggtacct tcttctggaa agtcggcgat
    14941 accgccatta tcgacaacgg tattgtcaac ggctctgcca gactggtcgg cgcggttgcc
    15001 gcgcaagtgc gtaaagtcca aacaggcttt atctacacct acgctgccgc tatggtgttc
    15061 ggcgtattgg tcttgctcgg tatgaccttc tggggattgt tcagataaaa atgagatttt
    15121 cagacgacgt ttaggtcgtc tgaaaaaaga aacttgagaa acaatgtcaa acacacaaac
    15181 catccgctct gccgacttca ccacttcccg cgcatggggc gcactcgaca tcgccaatat
    15241 gaacggcacc accgtgcgcc tgcactggac ggaccagccc tacaaatggc acgtcaacga
    15301 cggcgaggaa gtgtttgccg tgatggatgg cgaagtcgat atgcactacc gagaaaacgg
    15361 cgaagaacac atcgttcggt tgaaaagcgg cgacatcttc tacgcaggaa tcggcgcgga
    15421 acacgtcgcc cacccgcgcg gcgaaacgcg gattttagtg attgagaaag aaggcagtgt
    15481 ttagggcgga tacgcataag tattatgtgc tttttcaggt atcctgaaaa tcataaatct
    15541 atcaacgaga ctttaatcat ggcaaatcta aatgtgagta gtaaaaaaat tattaatctg
    15601 ctaggtaatc aagatatgca ggaaaaatat tttatcattc ctgaatatca acgcccctat
    15661 cgctgggata ccgaaaaatg cgccgtatta tggtctgata ttgtggattt tcgccagtcg
    15721 tccacaggaa aaaatgaaga atattttctt ggtacaatcg ttgtttgccc caatgaaaaa
    15781 ggtaatttgg aagtaattga tgggcaacag cgtttaacat cattactgct gttattgcgt
    15841 gcgttttatc gagttttaga gcaaacggaa agtacccccg ataccgcagc caaaattacc
    15901 acacttaaaa gcaaaattgc cccctgtatt tgggatgttg atgttctaac ggctgaaatt
    15961 accgatagaa ccgcaatccg gctgcatacg caagttgcca cagaaacaga taacgaaatt
    16021 ttgcacgaga ttttggcaaa cggtgaaatc tcagaagagt gtcgttctaa ttacgccgtt
    16081 aattatttgt atttcctgaa acagtgccaa gaatacgcca gaaccaaccc aatggattgg
    16141 tatcatcttt gtttaactat tttgaatgac tgtattttac ttccgattga aagtgacagt
    16201 ttaaatagtg cattaaccat attttccaca ttaaacgata ggggcttgcc tttgtcggat
    16261 gcagatattt ttaaagccag gctttataag atggcgcagc agaaagataa atttgttgaa
    16321 caatggaagg aacttgttgc gttggcagag ggtgcatcta tttccgtaga tgatttgttc
    16381 cgctattaca cccatgtatt aagggcgaaa gaaaagatta cggataaaga aattgggctt
    16441 cgccgttttt atactgacaa ccgaaacaaa cagttggaaa acgaaaattt gttcaatcac
    16501 ttaattgagc tggctggatt ttggcaggat attaacaaca gcaaaactaa ccaagacgtg
    16561 gaaacttcga actttgtaaa tgacgaggcg gccaagtggc tgcaatgtct gaatgtctat
    16621 cctaatgagt tttggaaata cgccgcgagt gtgttcttca ttgcaaacaa gggtgcgcaa
    16681 tcctttaaag atgattttgc agcattatta aaacagctta ctgcattttt gtatgctaaa
    16741 tttttagttg cgccaaccgt aactgcaatt aaagatgata tttataaatt atgtgttgcg
    16801 atttatcacg gtgaaaaaaa cgctttatca tatgttatgc ctgcacccat acactatgaa
    16861 caaaatacag gacgtataac taaagggttg ttaatgatta atgcttatct atttgatgaa
    16921 aagcagaaat tattgccaac taaaactgaa atcgaacata tctttccgaa aaaatggcag
    16981 gaaactaact ataatggttg gtcaatcata gatgcggaac aatatttaga acaactaggt
    17041 aataaaattg tttttgaaaa acggttaaat attcaggcag gtaacggcta ttttggtaag
    17101 aaaaaggagc aatatcaaaa atccgatatt gttgaagtgc gtgaattagc aggtgaaaat
    17161 gccaatgatt ggttaccgag tgatattcag aaacgccagc agcagattgt ggatagattg
    17221 aaacagtttt tcgataattg ttttgaagca gccagcatcc ccaaaaaaaa ttaacttttc
    17281 caataacctc caaaccaaac aggtaacgca atgttttcta accatcttct cagcttggca
    17341 atatggatac ccattgccgc aggtgtgctg gttttggcga ccggtaaaga cagccgtgcg
    17401 ccgctggcgc gtgtgcttgc cttcatgggt gcgcttgccg gtttcttggt aacgctgccc
    17461 ctgtttaccg gtttcgaccg tttgagcggc ggctatcagt ttaccgagtt ccacgagtgg
    17521 attccgcttc tgaaaatcaa ctacgcattg ggcgtggacg gtatttcagt gctctttgtc
    17581 atcttgaatg cgtttattac gctgttggtg gtattggccg gttgggaagt tattcagaaa
    17641 cgtccggcgc agtatatggc ggcattcctg atgatgtcgg gtttgattaa tggtgcgttt
    17701 gccgcgcagg atgcgattct gttttatgtg ttcttcgagg gtatgctgat tccgctgtac
    17761 ctgattatcg gtgtatgggg cggcccgcgc cgtgtctatg cgtcggtcaa gctgttcctc
    17821 tatacgctga tgggttcgct tttgatgctg gttgccatgg tttacctgta ctatcaaaca
    17881 ggcagcttct ctattgtcga tttccaaaac atcaaacaga ttccattggg cgtacaacag
    17941 cttttgtttg tggcgttctt cctgtcattc gccgtaaaag tgccgatgtt ccctgtgcac
    18001 acttggttgc cggatgccca cgttgaagcg ccgaccggcg gttcgatggt attggcggca
    18061 attacgctga aactgggtgc gtatggtttc ttgcgcttta tcctgccgat tatgccggat
    18121 gcggcacgct attttgcccc tgtaatcatc gtattgagcc tgattgccgt gatttatatc
    18181 ggtatggtgg ctttggtgca aaccgatatg aaaaaactgg tggcgtattc ttccatcagc
    18241 catatgggtt ttgtcacttt gggtatgttc ctgtttgtga acggccaact gaatgactgg
    18301 gcattgaaag gcgcaatcat tcaaatgatt tcccacggtt ttgtgtccgc cgcgatgttt
    18361 atgtgtatcg gtgtgatgta cgaccgtctg catacccgca atattgccga ttatggcggc
    18421 gtggtcaatg tgatgcctaa gtttgcggcg tttatgatgt tgttcggtat ggccaatgcc
    18481 ggtctgcctg caacttccgg cttcgtgggc gagtttatgg tgattatggg cgcggttaaa
    18541 gtgaatttct gggtaggcgc gttggctgcc atgaccttga tttacggtgc gtcttatacc
    18601 ctgtggatgt acaaacgcgt tattttgggt gcgatccaca atccgcacgt tgccgaaatg
    18661 aaagacatca attgccgcga atttgcgatt ttggcgattt tggcggttgc tgtattgggc
    18721 atgggtctgt atccgcaagc gtttatcgaa gtggtgcatc aggcggcaaa cgatttgatt
    18781 gcccatgtag tgcaaagcaa gatttgaggt gtgtaaatga actggtctga tttgaattta
    18841 atgcccgcca tgcccgaaat cgtgttgctt gcgctgctgg tgttattgtt gttggcggac
    18901 ttgtgggtca gtgatgacaa acgccgttgg acgcattacg gcgcgttggc aacggtggcg
    18961 gttacggcgg cggtgcagct tgccgtgtgg gaacagggca gcacgtcttc gttcaacggg
    19021 atgtatattg cggacggtat gtcgcgtttg gcaaaaatgg ttttatatgc cttgatgttt
    19081 accctgtttg tctatgccaa gccctacaac caagtgcgcg gtatttttaa aggcgagttt
    19141 tacaccctgt cgctgtttgc cctcttgggc atgagcgtga tggtgagcgc ggggcatttt
    19201 ttaactgcct atatcggttt ggaactcttg tcgcttgccc tttacgccct gattgccctg
    19261 cgccgcgatt ccggctttgc cgccgaagcc gccttgaaat attttgtttt gggcgcgctg
    19321 gcatccggcc tgctgctcta cggtatttct atggtttacg gcgcaaccgg ttcgctggaa
    19381 tttgccggcg tgctcgcctc ttccttcaat gaagaagcca atgaatggct gttgaaactg
    19441 ggtctggtgt ttatcgtcgt cgccgttgcg ttcaaactcg gcgcggtgcc gttccatatg
    19501 tgggtgcccg acgtgtatca cggcgcgccc acttctgtta ccgccttggt cggcactgcc
    19561 ccgaaagtcg ccgccgtcgt tttcgctttc cgcatcctcg ttaccgggct gggaaccgtg
    19621 catcatgact ggtctctgat gtttgccctg cttgccgccg cctcgctgct ggtcggcaac
    19681 cttgccgcca tcatgcagac caatatcaaa cgtatgctcg cctattccac cgtatcgcat
    19741 atgggtttca tcctgttggc gtttatggcg ggcgcggtcg gctttgcggc gggtctctat
    19801 tacgccatta cctacgcgct gatggcggcg gcagggttcg gcgtgttgat ggtgttgtcg
    19861 gacggggaca acgagtgcga aaacatcagc gatttggcag ggttgaacca acaccgcgta
    19921 tggcttgcct ttttgatgtt actggttatg ttctctatgg cgggcattcc gccgctgatg
    19981 ggtttttacg ccaaattcgg cgtgattatg gcactcttga aacaaggcta tgtttggttg
    20041 tccgtatttg ccgtcgtgat gtcgctgatt ggcgcgttct actacctgcg cgtggtcaaa
    20101 gtcatgtatt ttgacgaatc cggccgcgcc cgtcccgccg tcggcggcaa caatgccgca
    20161 aaattccttt tgagcgtcaa cgcgctgctg ttggtgctgt ggggcattat gccgcaaacc
    20221 gttatcgact ggtgcgccaa ggcgttggaa aacacgctgt aagccgccgc aacggcagcg
    20281 gcgtcagagg ctgccgtttt tgttaagata tgccgttccg caatgcagtt cagacggcat
    20341 cgccgccgac aatgcccaaa cagaaagccc gccatgaccg catccatgta catccttttg
    20401 ctgcttgcct tgatttttgc caacgccccc ttcctcacga ccagactgtt cggcatcgta
    20461 ccgctcaagc gcaaacattt cggacaccac ctgatcgagc tggcggcagg tttcgcgctg
    20521 accgccgttc ttgcctacat cctcgaatcc cgtgcgggag cggtacacga tcagggttgg
    20581 gagttttacg ccaccgtcgt ctgcctgtac ctgatttttg cgtttccctg tttcgtgtgg
    20641 cggtattttt ggcacacgcg caacagggaa taggcaggca taggaatgcc gtctgaaacc
    20701 ctttcagacg gcatttgttt cattcaagtg caggccggca tcgctgtgcc ggcacgtttc
    20761 agccggcgat atacgccggt tttaatattt gcgggcgact gcaaattctg ccaactgccg
    20821 caggcgcagg gctttgtcgc cgaagggttc gagcagcgcg accgcttcgg caaccagttt
    20881 gtgtgcgtat gagcgcgccg cttccaagcc catcagtctc acataagtcg gcttgtcgtt
    20941 gtctgcgtct ttgcccgccg ttttgcccaa agtcgccgtg tccgcttcac aatccaacac
    21001 atcgtcaatg acttggaacg ccagccccag ttttgccgca taatcgtcca ataccgccag
    21061 actgtcttca gacagattcg ggcaggacat cgcccccaat aaaaccgccg cacggattag
    21121 cgcacccgtt ttcaggctgt gcatctgttc caaatcggct tgaaccattt gtttgccgac
    21181 attcgccaaa tcgattgcct gaccgcccgc catgcccgcg ctgccgcctg cttttgccaa
    21241 aactgacaac atcgccagct gccgcgaagc aggcaggccg gacggacgcg ccaatacatc
    21301 gaaggcttgg gtttgcaaag cgtcgccgac cagtagcgcg gtggcttcgt cgtattgtat
    21361 atggcaagtc ggtttgccgc gccgcaggct gtcgttgtcc atcgccggca tatcgtcgtg
    21421 aaccaaagaa tagacgtgga tcatttcgat tgccgccatt gcctgttcta ctgcttcatg
    21481 cacggcttcg cctaattccg aagctgctag aaccagcatc ggccgcagac gcttgccgcc
    21541 gtccaaagcc gcatagcgca tcgcttcgtg cagcgtgtgc gggatttcgt tttcagacgg
    21601 caaaaagcgt tccagcagca gctctgtctg tgcctgcgcc ctctgttgcc acgctttcaa
    21661 atcattcgtc ggattcaagg ttcaactcct tcagcccgtc cacatccaaa acctgtaatt
    21721 tctgttcgac ttgcgccagc ttggtttggc agtatctgac cagctcgttg ccttcctgat
    21781 aggcggcaag cgcgtcttcc aagggcattt cgccctgcat agcttgtgtc agcgattcaa
    21841 ggcgcgacaa ggcttcttca aacgatttcg gggcgttttt cttcatcgtg tttccttttc
    21901 ggttgaaacc ccgccacttg gacatctgtc cttcggggcg gtagaatcag actttatttg
    21961 ggaggggtgt aagcccttcc aaatcaggac gacacatagg gcggtgcttt aagtgtcgtc
    22021 ctgcgtgttg gaacatagtt tcggatgttc cagtaaaaag cggattgtag catttttgaa
    22081 aaacggatgc cgtctgaaac ccgaatccgg tttcagacgg cattgttttg tcctgcaaac
    22141 ggcaaggcgt tacccgggca gttcgtcggt gatgccctgc aaaaaggcga ggcgttcggg
    22201 gcttgccgcc ctggtttcgg cggcggcttt gaaggcgcag ccgggttcgg cgcggtgggt
    22261 gcagttgtgg aagcggcatt gcccgacaag gtggcggaaa tcggggaaat agcgcggcaa
    22321 atcggcggct tggaggtggt gtaaaccaaa ttcttgcaaa cccggggagt cgatgagttg
    22381 ggtttcgccg ttcaaatcat aaagccgggc gtgggtggtg gtgtgttttc ccgagtcgag
    22441 tgcggcggaa atgtcgccgg tgcgggcggt ttggctgccc aaaagggcgt tggtcagggt
    22501 ggatttgccc ataccgctct gcccgagcag gatgttgctg tgcccttgca gggcggggcg
    22561 caggctgccg gcgttttcca gtgcgcgggt ttcgatgacg ggataaccca gcgtttcgta
    22621 gaatttgagt ttttcgcgcc aaagggcggt ttcgggcagg tcggctttgt tcaggacgat
    22681 gacggcgcgg atgccggcgg cttcggcggc aagcagggcg cgctgtagca gccgcacgct
    22741 cggactcggg acggcggcgg ttacgatgag gagttgggta acgttggcgg caatgagttt
    22801 ggttttccac gcgtcttggc ggtagagcag gctttggcgc ggtaaaaaat cttcaatcac
    22861 aacttgttcg gcgttgacgg ggctgatgcg gacgcggtca ccgcaggcga aatcgacgcg
    22921 ttttttgcgg gtgctggctt cgtaggttgt gccgtcgggc gtgcggacaa tgtagcggcg
    22981 gccgtagctg gcggtaattt gggcggtgtc gttcatggtt tctttggggt tgggtgtgga
    23041 aatgccgtct gaaaacgggt gttcggacgg catcggttca gtcgtgctgc cactcgacgt
    23101 gttcgttgag gaagccgccg ctctggtgcg cccagagttt ggcgtaaagc ccgcgttttt
    23161 cgaggagttc ggcgtgtgtg ccttcttcga tgatgcggcc tttgtcgagg acgacgagcc
    23221 tgtccattgc ggcgatggtg gagaggcggt gggcgatggc gatgacggtt ttgccgtcca
    23281 tcattttgtc gaggctttcc tggatggcgg cttcgacttc ggaatcgagc gcgctggtgg
    23341 cttcgtcgag cagcaggatg ggcgcgtctt tgagcatcac gcgggcaatg gcgatgcgct
    23401 ggcgttgccc gccggagagt ttcacgccgc gttcgccgac gtgcgcgtca tagccgcgcc
    23461 gccctttggc atcggaaagt tcggggatga agccggcggc ttcggcgcgt tcggcggcgg
    23521 aaaccatttc ggcatcggtc gcgtcggggc ggccgtaaat aatgttgtcg cgcacggaac
    23581 ggtgcagcag cgaggtatct tgcgtgacca aaccgatttg ggcgcgtaaa gattcttggg
    23641 tgatgccgct tatgtcctgc ccgtcgatcg aaaccgtgcc gctttgcggt tcgtagaagc
    23701 gcaaaagcag gttgacgatg gtggatttgc ccgcgccgct gcgtccgatc aagccgactt
    23761 tttcgcccgg gcggatggtg aggttgaagc cgttgagcag cggtttgccg gcttcgtagc
    23821 agaaatcgat gtgttcaaat ttgattgcgc cttgcggcac gttcagcggc agtgcccggg
    23881 gcttgtcgag gatggtgtgc ggtttggaca gggttgccat accgtcgttg acggtgccga
    23941 tgttttcaaa cagccgcgcg gattcccaca taatgtattg cgacagcccg ttgacgcgca
    24001 acgccatcgc ggtggcggtg gcgaccgcgc ccacgccgac ctgtccgttg tgccaaagcc
    24061 agatgcccag tgcggcggtg gagagggtca gggaggtgtt gacgatgaag ctgcacgaat
    24121 gcagcagcgt cgccagccgc atttgggcgc gcaccgtaac cataaattct tccatcgact
    24181 gcttggcata ggcggcttca cgcgcgccgt gggagaagag tttgacggtg gcgatattgg
    24241 aataggcatc ggtaatgcgg ccggtcatca gcgagcgggc atccgcctgc catgcggcgg
    24301 tttgccccaa tttgggaatc agcaggcgca tcaccgaagc gaaaccgaca atccagccga
    24361 taaagggcag cagcagccat gagtcgagcg aggcgagaat cacgccggag gtaatgaaat
    24421 acaccgacac ataaacgacc atatcggcaa ccgtcatcac cgcgtcgcgc aacgccagcg
    24481 cggtctgcat gactttggcg gacacgcgtc cggcaaattc gtcctgataa aaaccgaggc
    24541 tttggttcag catcaggcgg tggaagttcc agcgcaggcg catagggaac acgccctgaa
    24601 gggtttgcag gcgcacgttg gacgcggcat acgcccacac gaccgaaaac accatcatcg
    24661 ccgccattgc cgccagttcc caaccttttt cggcaaacag ttcggcgggc gcgtatttgc
    24721 cgagccactc cacgattttg cccataaatt gaaaaaccag ggcttccata atgccgatgc
    24781 cggcggtcag cgcagccagg gcggctatcc atttccgcac gccggccatg ctgctccaga
    24841 caaaccgcca caagcctttt tctggcgttt tcggggcggc ttcgggataa gggtcgattc
    24901 gggactcgaa ccaggaaaat attttgttca acattgtttt cgatttcggt aaaacagttt
    24961 cagacggcat caaacacaat gccgtctgaa aggaaggaca ataacgccat tttacgggaa
    25021 aagccgtcgg gaagacagcg cgaggcggaa acgcagggtt tcgtcagggc aaacgccgcg
    25081 ccgccttcag gcggcattat ttcagcaggt ttttcaaagc aaggcgcacg ccttcgccca
    25141 cgtccgtccc ctccggaacg cctttgaccg ccgcttttgc ttcgcgttcg ctgtaaccca
    25201 gcgcaagcag cgtgctgacg atgtcttccg tttcgtcggc ggcgggtgcg gcggcaaaca
    25261 gcccgtccgt taccgtatgc gcgaccagct tgccgcgcag ttccaagacc atacgttcgg
    25321 cggttttttt gccgattccc ggcgcggaag acaggcgttt gacatcttct tcggcaaccg
    25381 cccgcgccag ctcgtctgcc gtcatcgctg acaaaatgcc caaagccgtt ttcgcgccga
    25441 tgccgccgac cttaatcagt tggcggaagg tcttgcgttc ttccgcagtg gcaaaaccaa
    25501 ataaaagatg tgcgtcttcc cgaatgataa gctgggtaaa cagttgtacg ctttcaccca
    25561 cgggcggcag gttgtagaag gtctgcatcg atacgtcggc ctcatagccg acaccgttga
    25621 catcgatgac gatttgcgga gggttttttt caaccagttt gccggtcagt ctgctgatca
    25681 tgtgtgccga atcctgaagt gtcgggtgca aaatgccgtc tgaaaccggt tcgggcttca
    25741 gacggcacgg attgtatcaa attcagtcgt cgcggcggga ggaaatcacg cggccggtac
    25801 gggcatcgac aacgactttg tattcctgtc cgtttttgac gatttcgaca tcatagtgcg
    25861 gacggccgtt gtcgtgttcg agatcgatgt cggtgatttt gccgccgaca cgcgccaacg
    25921 ctgctttttc ggcttgggcg cggctgatga ttttgtcttg tttgttgtgt tggtgtgcgg
    25981 cgtgtccgtg gtcgtcatcg ccatgtccgt cgtggtgggc gagtgcgggg gcggaaatgc
    26041 ccatcagtgc ggttgcggcg gcggtcaaga gaaggcgttt gaagttcatg ttttgctcct
    26101 gcgaagtatc tggtggtgtt tgaaagacgt aaaggcggga catcaaccgg cagtcaatac
    26161 cgcccgaacc ataccgcgcg cctgagcttc ggcttcggcg gcgcgttcct gcgaggcaaa
    26221 cggtcccatt ttgacgacgt attcgtaacg gcgtttttca accgagaggt tcgtacccga
    26281 tgacgaaacg gcgaagtttt gggcggcttg gttcagatag gcttgtgctt cgtgttccgt
    26341 accgaaagat ttcaagtcga taaagatgtc tttgttttcg gcaaccggtg cggattggcc
    26401 cgggacgatt tgttcgattt tgacgtgtgc cgtcccttgg ctgacaaagc ccaatttttg
    26461 cgcggcggct ttggatacgt cgatgatgcg gttgccgtgg aaggggccgc ggtcgttgac
    26521 gcggacgatg acgcttttgc cgtttttggt gttggttacg cgcacatagc tggggatggg
    26581 cagggttttg tgggcggcgg taaaggcgtt catatcgtat cgttctccgc cggaagtttt
    26641 gcgcccgtga aacctaccgc cgtaccacga ggcgttgccg gtttgcgtga attcggcgac
    26701 ttggtttttc ggcgtgtagc gttttccggc gactttgtag ctgcggttgg cggaggcgtg
    26761 cagtttttct gccttgacca ctgcgtcggc ggatgtcgtc tgaagggagt gtgtgccgaa
    26821 tgcggcggtg agaaggaaaa gggtttttcg ggttaaagtc aaaacgtgtt ccgttcttga
    26881 gttgaagacg aatgggcatc atgcccgccg gatacgttcc gaaccgccgt acagtgcgga
    26941 cggcggttcg gaatgtgtcc ggataggttt tcagacggca tgaacttgcg ttcaaacgcc
    27001 gcctgcgtaa ccgtgttgcc gccacgcttc aaagagaatc acggcaacgg tattggaaag
    27061 gttcatactc cggctgccgg gctgcatcgg caggcggatt ttttgcgcgg cgggcaggct
    27121 gtcgaggatg tcggcaggca gtccgcgcgt ttccggcccg aacagtaaaa cgtcgccttt
    27181 ttgaaacgcg gtttcatcgg ggtgcgccgt gcctttggtg gtcagggcga aaatgcgcct
    27241 gcctgcgagt gccttgaggc agtcgtcgaa gttttcgtac accgtcaggc tggcgaattc
    27301 gtggtagtcg agcccggcgc gtttcatttt ggcggaatcc aatgggaagc cgagcggttt
    27361 gacaaggtgc aaatccgcgc cggtattggc gcacaggcgg atgatgttgc ccgtgttcgg
    27421 agggatttcc ggctggtata aaacgatggt aaacataaat atcaatcact tataggcgcg
    27481 taaccttgcc acaaggcgga tggggtgtca aaaaatttag ttattttttc attggcgtgc
    27541 gtgccagcgt ccagcagcag attcggtttg cgtccgattg tttcagcgtc tttgccaatt
    27601 cgtccagcgt cgcgccggtg gtaaagacat cgtcgattaa caaaatatta cagttttccg
    27661 gtatcggtgt gcggatttca aaggcgtttt tgatgtttcg ccgccgttcg ctgcctttga
    27721 gcgtgctttg cggcgggcgg tggtgtcgga atacggtgtg tcggggcagt atccgccagc
    27781 cgtagcgttg cgccaacaac ccgacgatgc tttcactttg gttgaacccg cgttgcagca
    27841 gccgctccct gcttagcggt acgggcagga cgaaatcgaa acattcgtct gcaagccggt
    27901 cgggcggatt ctgcatcatc aggtctgcca gcggctgcac catgctcaaa tcagccaagt
    27961 gcttcagcgc gtgtatcata ttgctgacgg gcggttcgta atgcagcgaa gcccacatcc
    28021 ggtcgaatgc gggcggtttt ttctgacagc cgccgcacac cgcgccgcct tggatgtgtc
    28081 tgaaacacag ggggcagctg tttgccgcgt cggtgcggta tgccgccaaa tccgccgcgc
    28141 agccggcgca gatgccgtct gaaacgccgg acgaaccgtg gcataatacg caacgcctga
    28201 tagtgggcgc gtctgcgatg cgccgccaac aagagagaaa attcatgcct gatgctgcca
    28261 aaaaagttta cctgatacac gggtgggggg cgaaccgcca cgtgttcgac gacctgatgc
    28321 cccgtctgcc tgcaacgtgg ccggtgtccg ccgtcgattt gcccggacac ggcgacgcgc
    28381 ccttcgcgca acccttcgat attgaggcag ccgccgacgc tgttgccgca caaatcgata
    28441 cgcccgccga cattctcggc tggtcgctcg gcggattggt cgcgctgtat ctggcggcgc
    28501 gccatcccga caaagtccgt tcgctctgcc tgacggcgag tttcgcccga ctgacggctg
    28561 acgaagacta tcccgaaggg cttgccgcgc ctgcattggg caaaatggtc ggcgcgttcc
    28621 gaacggatta tgccaaacat atcaaacagt ttctacaatt acagcttttg catacgcatg
    28681 atgcggacgg aatcataggc agaatcctgc ccgatttggc gcgctgcggc acaccctccg
    28741 ccttacagga agctttggac gcggcggaaa gggcggatgc gcggcatttg ttggacaaga
    28801 tagatgttcc ggtactgctg gtgttcggta gcaaagacgc gattacgccg ccgcgcatgg
    28861 gcgagtatct gcatcggcat ttgaaaggaa gcaggctggt tgtgatggaa aaggcggcac
    28921 acgcgccgtt tttgagccat gcggaagcgt ttgccgcgct gtaccgcgac tttgtcgaag
    28981 gggtttgaca tgaatccgca ggacaaatgc tggcaggttc accgccatct cgccgaatat
    29041 gccgaccaac gcctgacact cgtccgcaac gcgcccaaac atatcctgct tgccggggcg
    29101 gatgcggaca tcagccgcag cctgctggcg aaacgctatc cgcaggcggt atttgaagaa
    29161 tacgattccc gtgcggattt tttggcggcg gcggcagccg cgcgcaaagg cggtttttgg
    29221 caaaggtttg cgggtaaggg cgtggtgcag cactgccaat ccccgaccgc gccgctgccc
    29281 gaagcgtgtg ccgatatgtt gtggtcgaat ctcggactgt tggcggcgga aagcatcgtc
    29341 cccgtgctgc aaaattgggc gcgcgcgctg aagacggacg ggctgctgtt ttttacctgc
    29401 ttcgggcgag ataccttggc ggaactgaaa tgccgtctga aagaaaacgg cattgaaagc
    29461 cgcagcgtga tgtttcccga tatgcacgac ttgggcgata tgcttgctga aaacggcttt
    29521 tacgaccccg ttaccgatac ggcgaagctg gtgttggatt acaaaaaggc ggaaacgttt
    29581 tgggcggata tggacacgct gggcgtttgg cgggcgatgg cgtggaacga tgaaaacgcc
    29641 gcgcgttcgt gtgtcgggac aatatttgaa cgggaaggcg gtttgggcat tacgctggaa
    29701 acggtgtacg gacacgccgt gaaaaaactg atgctgccgc aaggggagaa cgtggtgcag
    29761 ttttttccga agagatgatg tgcagatgcc gtctgaagcc gtttccaggt ttcagacggc
    29821 atctgtctgt gaaaaccgac agaaataaag gaaataccga tgtatagtg
//
LOCUS       DAWFJR010000017        29376 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWFJR010000017 DAWFJR010000000
VERSION     DAWFJR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29376
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.017"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..676
                     /locus_tag="WGR04_000669"
     CDS             <1..676
                     /locus_tag="WGR04_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0794276.1"
                     /translation="AHIRAGDRVLSKDEASGETGYKPVTARYGNPYRETVYIKVSDGI
                     GKIQTLVSNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITFKQQPLKAYNLT
                     VADWHTYFVKGDRAETEGVWVHNDCPYGEKYRTEVGSYTNTHESGRTYSGKGTRQRSQ
                     ISGAREARVNNDPHIATDFTPAKNNREAFKDESRRIDAHGGSKSTNNYNRIESPGKKY
                     RKQDGD"
     gene            679..1002
                     /locus_tag="WGR04_000670"
     CDS             679..1002
                     /locus_tag="WGR04_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEZ0794277.1"
                     /translation="MIVDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     RLISYRQDFQISIEQNEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            1061..1288
                     /locus_tag="WGR04_000671"
     CDS             1061..1288
                     /locus_tag="WGR04_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794278.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            1415..1957
                     /locus_tag="WGR04_000672"
     CDS             1415..1957
                     /locus_tag="WGR04_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ0794279.1"
                     /translation="MINHIEKYTGTISHGSKSDSGSQYKLNIAAIPSSPNRDLKTYIT
                     LGLSKYNLNYKSRFEILFVCSLKYDENQIFPFLRWLAETIIENKKILLRGQVIYLPRS
                     IVDSTKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFEQ
                     FLLDNEVDNLSDMNRKPFAW"
     gene            2405..2757
                     /locus_tag="WGR04_000673"
                     /pseudo
     CDS             2405..2757
                     /locus_tag="WGR04_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2939..3328
                     /locus_tag="WGR04_000674"
     CDS             2939..3328
                     /locus_tag="WGR04_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794280.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLF"
     gene            complement(3369..4826)
                     /locus_tag="WGR04_000675"
     CDS             complement(3369..4826)
                     /locus_tag="WGR04_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ0794281.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(4841..5671)
                     /locus_tag="WGR04_000676"
     CDS             complement(4841..5671)
                     /locus_tag="WGR04_000676"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ0794282.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRIAKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            complement(5749..6138)
                     /locus_tag="WGR04_000677"
     CDS             complement(5749..6138)
                     /locus_tag="WGR04_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0794283.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            complement(6274..6798)
                     /gene="nspA"
                     /locus_tag="WGR04_000678"
     CDS             complement(6274..6798)
                     /gene="nspA"
                     /locus_tag="WGR04_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ0794284.1"
                     /translation="MKKALATLIALAIPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSAGVRVKF"
     gene            complement(6905..7165)
                     /locus_tag="WGR04_000679"
     CDS             complement(6905..7165)
                     /locus_tag="WGR04_000679"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794285.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKNRMPAPATIRQIP
                     LFFPNRLQVSPIRRVMPPPPFKGNARVNGFAVGKAAGYRYVS"
     gene            complement(7385..8584)
                     /locus_tag="WGR04_000680"
     CDS             complement(7385..8584)
                     /locus_tag="WGR04_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ0794286.1"
                     /translation="MSTFPNHTMHAISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDF
                     NSHSLKNGLPEAAYIDALLTDLQLELPNIWGRPVETVFFGGGTPSLFQAESIDRLLSG
                     VRSLLRLQPEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHN
                     GKEALTAIATALKLFDKINIDLMYALPNQTVRTALDDVQTAIATGATHISAYHLTMEP
                     NTPFGHTPPKGLPQDEAALDIEDAVHGALESAGFVHYETSAFAKPAMRCRHNLNYWQF
                     GDYLGIGAGAHGKISYPDRIERTVRRRHPNDYLALMQNRPSEAVERKTVAAEDLPFEF
                     MMNALRLTDGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLN
                     DLLQCFL"
     gene            complement(8617..11142)
                     /gene="ligA"
                     /locus_tag="WGR04_000681"
     CDS             complement(8617..11142)
                     /gene="ligA"
                     /locus_tag="WGR04_000681"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ0794287.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPEFKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVANIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEQMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAET
                     PSANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETTDKTVAESDQTPSEG
                     SSVGASGKHKKQPVKWAENILGGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVAHSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPP
                     ARHAEPQRWIARLPDFKISENKAQALWELAGKNIEGLQNDKALPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSILSEAELLTLLG"
     gene            complement(11290..12576)
                     /locus_tag="WGR04_000682"
     CDS             complement(11290..12576)
                     /locus_tag="WGR04_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ0794288.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGQPSGGPVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(12770..13342)
                     /gene="ampD"
                     /locus_tag="WGR04_000683"
     CDS             complement(12770..13342)
                     /gene="ampD"
                     /locus_tag="WGR04_000683"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ0794289.1"
                     /translation="MDNHAEAHWQDGWLQSVRHIPSPNFGPRESKEEVSLVVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRGGETVQFVSCDNMAYH
                     AGVSSFGGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIREKGFPVDRNAV"
     gene            13426..14421
                     /gene="mltG"
                     /locus_tag="WGR04_000684"
     CDS             13426..14421
                     /gene="mltG"
                     /locus_tag="WGR04_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ0794290.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLVEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            14481..15101
                     /locus_tag="WGR04_000685"
     CDS             14481..15101
                     /locus_tag="WGR04_000685"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ0794291.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAIIDSNRSLDEVRNSIEKVLDGHFGC"
     gene            15323..16603
                     /locus_tag="WGR04_000686"
     CDS             15323..16603
                     /locus_tag="WGR04_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ0794292.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIASLEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRYTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYKTREDKDRMDESKQFYAIEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            16839..17110
                     /locus_tag="WGR04_000687"
                     /pseudo
     CDS             16839..17110
                     /locus_tag="WGR04_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17150..18184
                     /locus_tag="WGR04_000688"
     CDS             17150..18184
                     /locus_tag="WGR04_000688"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ0794293.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPSADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            18390..18971
                     /locus_tag="WGR04_000689"
     CDS             18390..18971
                     /locus_tag="WGR04_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ0794294.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAKAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            19040..19222
                     /locus_tag="WGR04_000690"
     CDS             19040..19222
                     /locus_tag="WGR04_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ0794295.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARPLGEEELEA"
     gene            19219..19980
                     /gene="kdsB"
                     /locus_tag="WGR04_000691"
     CDS             19219..19980
                     /gene="kdsB"
                     /locus_tag="WGR04_000691"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ0794296.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPFETAVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTI"
     gene            20003..20320
                     /locus_tag="WGR04_000692"
                     /pseudo
     CDS             20003..20320
                     /locus_tag="WGR04_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            20425..20622
                     /locus_tag="WGR04_000693"
     CDS             20425..20622
                     /locus_tag="WGR04_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794297.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDVAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            20779..20871
                     /locus_tag="WGR04_000694"
     tRNA            20779..20871
                     /locus_tag="WGR04_000694"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20813..20815,aa:Ser,seq:gct)
     gene            21297..22082
                     /locus_tag="WGR04_000695"
     CDS             21297..22082
                     /locus_tag="WGR04_000695"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ0794298.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAKAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNTGNEAAAVGALVKELKDAVR"
     gene            22120..22992
                     /locus_tag="WGR04_000696"
     CDS             22120..22992
                     /locus_tag="WGR04_000696"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ0794299.1"
                     /translation="MSWLDKILPPKIKNRGKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEIAGNVKPTDPLKFKDSKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGIRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPESFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(23052..23663)
                     /locus_tag="WGR04_000697"
     CDS             complement(23052..23663)
                     /locus_tag="WGR04_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ0794300.1"
                     /translation="MRRTALLLLALAAGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSIKPHNK"
     gene            23772..23996
                     /locus_tag="WGR04_000698"
     CDS             23772..23996
                     /locus_tag="WGR04_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ0794301.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVQHK"
     gene            23997..24602
                     /locus_tag="WGR04_000699"
     CDS             23997..24602
                     /locus_tag="WGR04_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794302.1"
                     /translation="MPSEMRMSRLMLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLF
                     RSACVLTQGSFESVSKFAAVGHFVPVGKEDLSHLPPTVAEPDVQALWTLERDGGIYYL
                     SLTRDSCSVKAERADSAALLEHFDGLVRQPPKNANIELRAEQSVQASFEIRQVSYAWR
                     EAGSSEETVLTAQTADSPDLPVQAVLNLTHRSHNGKPLILP"
     gene            24662..25696
                     /gene="pyrC"
                     /locus_tag="WGR04_000700"
     CDS             24662..25696
                     /gene="pyrC"
                     /locus_tag="WGR04_000700"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ0794303.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPALVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENTDTITLVKQSQTVPASIPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(26062..26487)
                     /gene="nusB"
                     /locus_tag="WGR04_000701"
     CDS             complement(26062..26487)
                     /gene="nusB"
                     /locus_tag="WGR04_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ0794304.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(26565..27041)
                     /locus_tag="WGR04_000702"
     CDS             complement(26565..27041)
                     /locus_tag="WGR04_000702"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ0794305.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNLASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(27089..27412)
                     /locus_tag="WGR04_000703"
     CDS             complement(27089..27412)
                     /locus_tag="WGR04_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ0794306.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            27579..28298
                     /gene="rnc"
                     /locus_tag="WGR04_000704"
     CDS             27579..28298
                     /gene="rnc"
                     /locus_tag="WGR04_000704"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ0794307.1"
                     /translation="MKDDVLKQQAHAAIQKRLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDTFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            28345..29268
                     /gene="era"
                     /locus_tag="WGR04_000705"
     CDS             28345..29268
                     /gene="era"
                     /locus_tag="WGR04_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ0794308.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
BASE COUNT         6845 a         6862 c         8522 g         7147 t
ORIGIN      
        1 tgcccatatc cgagccggag accgcgtcct ttccaaggac gaggcaagcg gagaaacggg
       61 atacaaaccc gttaccgccc gatacggtaa tccgtatcgg gaaaccgttt acattaaagt
      121 ttcagacggc atcggtaaaa tacagaccct cgtttccaat aaaatccacc cgttttacag
      181 tcaaggaaaa tggatacagg caggtcgtct gaaaaaaggc gacaccctgc tttccgaaag
      241 cggcgcaaaa cagacggttc aaaacattac cttcaaacag cagccgctca aagcctacaa
      301 tctgaccgtt gccgactggc atacttactt cgtcaagggt gatagggcgg aaacggaagg
      361 ggtttgggtt cataatgatt gtccgtatgg agaaaaatat aggacagaag ttggttctta
      421 tactaatact catgaaagtg gtcgaactta ttctggaaag ggcacaagac aacgttccca
      481 aatctctgga gcaagagaag ctagggttaa taatgaccct catatagcta cagattttac
      541 tcctgccaaa aataatcgag aggcattcaa agatgaatct agaagaattg acgctcacgg
      601 tggttccaaa agtaccaata attacaatcg aattgaaagc ccggggaaaa aataccgaaa
      661 acaagatgga gattaaaaat gattgttgac gaaaaatcta cttcagatgt actgaaacaa
      721 tatcaagaac tagggtttga tttgacaaaa cctatgataa tagagttttt tataggaggt
      781 tctcaaaaga atctgcaatc aatagaaagc cgactcattt cttatcgaca agattttcaa
      841 atcagtattg agcaaaatga attcggagaa atgtggactt gctattgttc cgtcaatatc
      901 gtcccttccc ttgaaaacat tttagctatt gaaactacgt tatttggtat tgcacaaaaa
      961 aatgattgta attatgaagg ttttggttcg tatgggaact aacaaaaagt gtaggttggg
     1021 ttgaaaaaac catattccca ttaaagatta gggtgtgtct atggacataa aattcaatac
     1081 tttgggggtt atcctgaatg gggtcaatcc tgaagaaaaa ttcataaaaa ttattgatga
     1141 tcaggaaaat accgggggat ttctgatact gttatcttca aatgacaaat tctcatcatt
     1201 tgacagttac gatgactggg tcgaaaactt agaaatatta aaagaatacc tgcaagaatc
     1261 tcattggata ataaagtggg tcggatagtc ggcaaacgta gtccgtctga aacactttta
     1321 cttttcagac ggactttcta tcaatgggga caaggcgttt tgtctcaatc tggtctatga
     1381 ttattcgata cagtgatggg aagctaagaa aaatatgatt aatcatattg aaaaatatac
     1441 aggcactatt tctcatggct cgaaaagtga ttcgggaagt cagtacaaat taaatatcgc
     1501 agctattccc tcttccccta acagggattt aaaaacttat attactttag ggttaagcaa
     1561 atataatctg aattataaaa gcaggtttga aatacttttt gtatgttctt taaaatatga
     1621 tgaaaatcag atttttccat ttttaagatg gttggcagaa accatcattg agaacaaaaa
     1681 aattcttctt cgaggacagg ttatttattt gcctagaagc attgttgatt caacaaaaat
     1741 ggatgcactg tatgtttccg ccccatttta tttcgatgat gattttcagg tctgttatgg
     1801 cgaacactac aatatcgttt tccctttgct tgtcccgttg tataaacagg aagcagaatt
     1861 ggtggaaaaa aagggttgga atgcttttga gcagttcttg ctggataatg aagttgacaa
     1921 cctttcggat atgaatcgga aaccgtttgc ttggtaagga ttttcttaaa tttaatcaaa
     1981 gtgccgtctg aaaccgtgtt taattttcag gcgaccttcc ccttatttta aacaggatat
     2041 tgggaactaa gttcttcaaa aatcctacac ctgctccttc cacggcagca ccttggtcaa
     2101 aacggcagac ggctgaaaag caaacaccgt ccgtcgtgtt gccgtttgcg gatgagtacg
     2161 ggtcaacccc aatgccgccg aaaccgtcga agccgccttc aacattgccg ccgccaaagc
     2221 cgcaaagttg gcaaaaacgg taaaaccggg gagataaaag ccgatggcag gaaagtaaat
     2281 gtgaggatag acagtacgga ggcagacctg ctttatccgg cagggcaata agaaaacaaa
     2341 aattagatat ggaaaacgat tgtgtaaacc attacaattt tctaacaata atcacagatt
     2401 ttatgtggac aaaatttgtg ttaccgaaca agatgtgaac attttggcat ccgaccgaaa
     2461 ttatgaatta aatattgaaa tatttattga caatataatt cattttcaaa taacagatga
     2521 atcttataaa gtaaaatttt cagaatattt atttgaaaat aaagaaaaaa atgattggga
     2581 tagaaatcct gctataaatt attttttcga gataatagat gacagttata tggactggtt
     2641 gaaagaagaa agttttgatt ttttgaaaag aaatattata aggcttatat tttctttttt
     2701 agcgattctg taatagaagt tatcagctcg acagaacctg tattttattc aaaataacaa
     2761 attatcaaac aaagctctga ttaaaaaccc aacaatcaaa atgccgtctg aaacaatatt
     2821 cggctttcag acggtatttt tgacacaaag caggtaacca aaggagtgtt tgacggaaaa
     2881 ggagaagcta aaataccgga tgtatcggtt gggaagcaat ggataaaggt aaataattat
     2941 gtggaaaatt agtaaagaaa attgtgaaga tttaggattt gcaatagtct gtatgttcta
     3001 tgatgctatt aatctttctg aatttaaatt atggttggat atagttgtca gagatactcc
     3061 tattgataca attccattgt atatttttga tttgattgat tttgataaga gtatagggga
     3121 aatttatgat gtaattggag tcgttaatta tggttacatt tcaaatgatc aaaaaaatgc
     3181 attaacgggc attgccttct taagggggat agatgtctat gatccgccta tttcaaaaga
     3241 aaaagcatta aaagccttag agaaatatcc tgaaatttat cagaggtttc agcatttctt
     3301 tccttttgta gagcttccgc ttttttaaaa gacaatatgc cgtctgaaaa gttttcagac
     3361 ggcatttttt atttcttcca gtaggcgggg gtgaagagga tgaagacggt gaagatttcc
     3421 agcctgccca agagcatggc ggtaacgcag atccatttct gcatcacgtc caaaccggcg
     3481 taattgccgg cgggcccgac ttcgcccagg ccggggccgg cgttggtgat gcaggcgatg
     3541 acggcggtga aggcggtggt aaattccata ccgctcgcca tcagcaggaa gctgaagagg
     3601 acgacggtca taaagtagat gaagatgaag gacataacgg tcagcgcgag gcggtcgggt
     3661 atggccttgc cgctgatttt gacggtgcgg acggctttgg ggtgcagcag caccatcatt
     3721 tcgcgcaggc tgaatttgaa caggacgagg gcgcgtatgg ttttgatgcc gccgccggtc
     3781 gagccggagt tggcgaggat gttggcgagg aaaaacatcc acagggaaat caggagcggc
     3841 cattgtgcga agtcggtgtt ggccagcccg tttgccagtc cgatggagac gaagttgaag
     3901 gcggtgtagc gcagggattc ggtaaaaccg gcgtaatagc cggtgtgcca caggtacagg
     3961 gcggcggcaa ggatgctgcc ggagagcagc agcagcatcg tccggcattc ttcgtctttc
     4021 caataggttt tgaggctgcg gctgttgagg gcggcgaaat ggttggcaaa attgatgccg
     4081 ccgacaatgg tgaaaacgat gatgaccgct tcgatgaggg gggagttgta ataagctatg
     4141 ctggcatcgt gggtggaaaa cccgcccagc gagagggtcg ccatcgcgtg acagacggca
     4201 tcgaaccagc ccatcccggc aaaatgcagg caggctgccg cgaggatggt gatcagggtg
     4261 tagccgaacc aaagtttttt cgccacttgg gaaatgcgcg gcgacatttt gctttctttg
     4321 tcaatgccgg ggatttcggc tttgaataac tgcgtgccgc ctacgccgag cataggcagg
     4381 atggcgacgg caaggacgat gatgcccatc ccgcccagcc agttgagcat atgccgccaa
     4441 aagttgacgg agggggcgag cccgtcgacg tgggggatga cggtcgcgcc ggtggtggtc
     4501 agtcccgaca tcgattcaaa aaatgcgtcg gtaaagccca tattcgggaa atacaggtac
     4561 atcggcatcg ccgccatagc ggcaaacgcc agccacaaca tcaggacgag ggtgaagccg
     4621 tcgcgcgggc gcagttcgcg cctgaaccgg agggtggcga gccggacgat gcacgagccg
     4681 gaaagggtaa cggtcgcggt ggtggcgaag gcggtgtacg cgccgtccga aaaggcgtag
     4741 gagagggcgg cgggtatcag caggataaag gaaaacagca tacccagtcg ggagaggacg
     4801 tgggcgatgg gcaggatttt gtgcatagtg gggcggtccg ttattttgcg aagcttttcc
     4861 agtctatgcc gccggcggct tggacttggg taatttcttc cgtttcgagg ttgacggcgg
     4921 tcagctgtcc gccccacagc gcgccggtgt ccagcgagat gacgttgtcg gcattcgtgt
     4981 agcccagcga ggaccagtgt ccgaagatga tggtgcggtc gaggttttgc cggtcggggg
     5041 ctttgaacca cgggcgcagg taaggcggca tttttttcac tgtggatttg tagtcgaaat
     5101 ccagttcgtt tttaaaggtc agggcgcgca tccgcgtgaa ggcgttgacg atgaagcgca
     5161 ggcgggcata gccttccaaa ccttcgtccc acgcggccgg tttgttgccg tacattttgg
     5221 agaagaattt gacgtatttt ttgccgcgca gttcggcttc ggcttctcta gcgagcgatt
     5281 cggctttggc gatgcgccat tgcggcagga tgccggcgtg taccatcacg cggctgccct
     5341 cgcgtatcaa cagcggttgc gcacgcagcc agtcgagcat tttttttccg tcggggtgtt
     5401 tgagtatggg ttcgattgtg tcgctgcgtt tgggcgcgcc ttcgccgcag ccgacagcga
     5461 gcaggtgcag gtcgtggttg ccgaggacga tttgcacgct gttttcgtgc cggatgcaga
     5521 attgcagcgt ttcgagggat ttcgggccgc ggttgacgat gtcgcccgtc agccagaggg
     5581 tgtccgtgcc gtggttgaaa ccgattttgc cgagcagcgc ggtcagttcg tcgaaacagc
     5641 cttgtatgtc gccgattgcg taatgtgcca ttgcagatgt tgtgaagtgg gaaagtgttg
     5701 cggttcggac ggcatggttt tgaaatatca tgcagtccga acgtggaatt atgcgttcaa
     5761 aacgaggacg gcttcggctt cgacctgcac gccttttggc agggaggcaa cgccgacggc
     5821 ggcgcgggcg gggaagggct cggcaataaa ttccgccatc acttcgttga agacggcaaa
     5881 attgcccaag tcggtcaggt aggcgttgag tttgacgatg tcggccagcg tgccgcctgc
     5941 cgcttcggcg acggcttgca ggttttggaa cacttggcgc gcttcggcgc ggaaatcgcc
     6001 gttgccgacg acggtcatcg tggcgggatc gagggggatt tgaccgctca tgtaaacggt
     6061 gtcgcctgct cggacggctt ggctgtacgc gccgatggcg gcgggggctt tgtcggtgtg
     6121 gatgatggtt ttggacattt cggattcctc aaaaaatagg gcggcagaag ccgcagcatt
     6181 cgggattatc gtacaaaacc gccggcttgt gtagttgcgg tggcagaaaa caaaaccgcc
     6241 gaaggctcgg cggtttgcag aataaggcgc atatcagaat ttgacgcgca caccggcgga
     6301 cagttcgccg gaacggacgt ttttgacagt gttgactttg ccgatgtagt tgtagcggta
     6361 gccggcatcc aaatcgacat tcggggtaac ggcatagctt acgcccgcca atacgccgag
     6421 gccggtggag gtttggctga agctgtcgct gccgcccaag tcgacggagg cgcggttgag
     6481 gctcaagcgc gcgccgagat acggtttgac gggcgattgg gtgtcgaagt cataaatggc
     6541 ggacgcgccg atgctgtaaa gtttgaaatc ggtggatggg gctttatagt ttttgtagcg
     6601 cgtgtaatcg acggcgaagc ggaggtcgtt gatgcggtag cctgcggaga tgcgcgggct
     6661 gaagcctttg gcagaaccta aagagcttga ggcttttgcg tgtgcggcat cggcttggac
     6721 gtaaaagccg gatgcgcctt ccgccagtgc ggcggccggg atagcgaggg caatcagtgt
     6781 ggcaagtgct tttttcatat tttggttcct ttatggtcag ttagaaaaat tgttaagaat
     6841 ccgttaaaga atcctgctgt attatactta aattttcttt ttgcatcgta atattttcaa
     6901 tacttcaaga tacgtagcgg tatccggctg ctttgccgac ggcaaagccg ttaacccgcg
     6961 cgttgccttt aaatggtggt ggcggcatca cgcggcggat gggtgaaact tgcaaacggt
     7021 ttggaaaaaa cagcggtatc tgtcggattg ttgcaggtgc aggcatacgg ttttttgtgt
     7081 gcgtctgtgc cttaagcgtc ggacatttcc ggcggcggct gtgccgtctg aaacgcccgg
     7141 cgggggatgc ggctgcgttt tccatcgata agcatatttt ccggacgcgt tcggggcggg
     7201 ttttcccggg cggccgccga tttgtttgcg cttatatagt ggattaacaa aaatcaggac
     7261 aaggcgacga agccgcagac agtacaaata gtacggaacc gattcacttg gtgcttcagc
     7321 accttagaga atcgttctct ttgagctaag gcgaggcaac gccgtactgg tttttgttaa
     7381 tccactataa aaaacattgc agcaaatcgt ttaaaaacaa gcgtcctttt tcggtcgggc
     7441 ggaatacggc ggggtcggtt tccagcaggc ctttttgcct tgctgtttcg atttgcgcca
     7501 tgattttggc actcggtacg cccgtgcgtt cctgcaacat cgcggcgggt acgccgtcgg
     7561 tcaggcgcag ggcgttcatc atgaactcaa acggcaaatc ttcggcggcg acggttttgc
     7621 gttcgacggc ttcgctcggt cggttttgca ttaaggcgag gtagtcgttg gggtggcggc
     7681 ggcggacggt gcgctcgatg cggtcgggat aggaaatttt gccgtgcgcg cccgcgccta
     7741 tgcctaaata atcgccgaac tgccagtagt tcaaattgtg gcggcaccgc atggcgggtt
     7801 tcgcaaaagc cgatgtttcg tagtggacaa aacccgcgct ttccagcgcg ccgtgtaccg
     7861 cgtcttcgat gtcgagggcg gcttcgtctt gcggcaaacc tttcggcggc gtgtgtccga
     7921 acggcgtgtt cggttccatc gtcaggtgat acgcgctgat gtgggttgcg cccgtggcga
     7981 tggcggtttg tacgtcatcc aatgccgtcc gaacggtttg gttcggcagg gcgtacatca
     8041 agtcgatatt gattttatca aataatttca aggcagtagc tatggcggtt aaggcttctt
     8101 taccgttgtg gacgcgcccc agccttgaga gcatatcgtc gttgaagctt tgtacaccaa
     8161 tagaaagccg cgtgatgcct gcgtctttaa atccttgaaa cttctcgatt tcaaatgtcc
     8221 ccggattagc ttccaacgta atttccgctt ccggctgtaa gcgcaacagc gaacgcacgc
     8281 cgctcaacaa acggtcgatc gattccgcct gaaacaggct gggcgtaccg ccgccgaaaa
     8341 ataccgtttc caccggcctg ccccaaatat tgggcaattc aagctgcaag tcggtcagca
     8401 gcgcgtcgat ataggcggct tcgggcaatc cgtttttcag gctgtgggaa ttgaagtcgc
     8461 aatacgggca ttttttgatg caccacggga tgtggatata gagcgacagg ggcggcaggg
     8521 cggtgagccg ggtgcggttt ggaaaggaaa tggcgtgcat ggtgtggttc ggaaaagtgg
     8581 acaatgccgt ctgaaggcgg ttcagacggc acaggttcag ccgagcaggg taagcagttc
     8641 ggcttcgctg aggatggaaa cacccaaggc attggctttt tccagcttgc tgcccgcggc
     8701 ttctccggcg acgacgtagt cggttttttt ggacacgctg ccggaaactt tgccgcctgc
     8761 ggcttcgatt agggattggg cttggtcgcg tttgagcgtg ggcagggtgc cggttaatac
     8821 aaaggttttg cctgctacgg ctttattgat gccgtctgaa ccttgcgccg cctcgtcttc
     8881 agacggcatt tgcgcgaaga aggttttcag gttttcgagt agggcggcgt tttgtggttc
     8941 gctgcgccat gcttgccagt cggcggggag ggctttgtcg ttttgtagac cttctatgtt
     9001 tttaccagcg agttcccata aggcttgggc tttgttttcg ctaattttga agtcgggcag
     9061 gcgggcgatc cagcgttgcg gttcggcgtg gcgggcgggc gggatggtaa cggcttgggt
     9121 ttgcggggca acgcctgcgg cgagcagttc gtctatcatc gcctgctgtt cggcttgggc
     9181 gaagaagtgg gcaatggaat gcgccactac cgtaccgatg tcgggcaggc aggcgaggac
     9241 gggttcgggg gcgcggcgga cgcgttccaa tgtgccgaat gcctgcgcca gcgttttggc
     9301 ggtgcgttcg ccgacgtggc ggatgccgag cgcgaacagg aagcgcgcga gttcgggcgt
     9361 tttgccggat tctatgcccc cgaggatgtt ttccgcccac ttgaccggtt gttttttatg
     9421 tttgcccgac gcgccgaccg aactgccttc agacggagtt tgatccgatt cggcaacggt
     9481 tttgtccgtc gtttctttca ttttttgcag ggtcggaata tcgaggcggt agaggtcggc
     9541 gaagtggcgg acgaggtctt gggcgacaag ctgttcgatt tgtttttcac ccaagccgtc
     9601 gatgtccatg gctttgcgcg aggcgaagtg gattaagcct tgcgcgcgtt gtgcctgaca
     9661 aagcataccg ccgctgcatc gggcgacggc ttcgccttct tcgcgttcga tttcgctgcg
     9721 gcagatgggg caatgggtcg gcaggcggta gggcttgtgg agcggaacgg attgggtttg
     9781 attagcggac ggtgtttcgg caaacaaatc gtcctgccga tgcccgatgc cgtctgaaac
     9841 agcaacggcg gtttcctgca tcgggcggcg ttcaaaaatc acacgcacga cttcgggaat
     9901 cacgtctcca gcgcggcgca cgacgacggt atcgccgacg cgcacgtctt ttcgggaaac
     9961 ttcgtcctga ttgtgcaggg tggcgttggt aacggttacg ccgccgacga atacgggttg
    10021 taatcgggca accggcgtta ccgcgcccgt cctgccgatt tgcacgtcaa tcgcttcgac
    10081 aatggtcagg gcttcttcgg cagggaattt gtgggcaatc gcccagcgtg gcgcgcgtga
    10141 gatgaagccg agttcgcgct gttgcgccaa gctgttgact ttgaccacca taccgtcgat
    10201 ttcgtagggc agttcggggc gtttttgctg catttgttcg taaaacgcca atacttcgtc
    10261 gatatttttg aaacagccga aattgccatt tggcagactg aagccgagtg cttggaaata
    10321 ggcgagttct tggatgtgtt cttccgcgac gaaaccgcct tgctggcggg cgacggagta
    10381 ggggaaaaag tgcagtttgc gttgcgcagt gatgcgcgaa tcgagttggc gcaggctgcc
    10441 ggcagcggcg ttgcgcggat tggcaaaggg tttttgcccg ttttcggctt gtcttttatt
    10501 gagggcggca aaatcggctt tttgcatcag tacttcgccg cgtacttcga tgagttcggg
    10561 cgtattttcg ccgtgcagcc gcagggggat gttggcgacg gttttgatgt tttgggtaac
    10621 gtcttcgccc gttgtgccgt cgccgcgcgt tgccgcctgt accaatacgc cgtcgcggta
    10681 gagcaggctg atggcgaggc cgtcgaattt gggttcgata acgtattcgg gattgccgcc
    10741 gtccaaaccg tcgcgcacgc gttggtcgaa agcgtacatt tctgcatggt cgaacacgcc
    10801 gttttcatct tgcggggaaa aggcgttggt cagcgacagc atcggcactt cgtggcgtac
    10861 ttcggcaaat cccgccagag gctcgccgcc gacgcgctgg gtcgggctgt cgggcagttt
    10921 gaattcggga tggtttaatt ccaacgcttc gagctcgcgg aataatttgt cgtattcggc
    10981 atcgggtacg ctgggcgcgt cgagggtgta gtattcgtag gcatagcggt tgagcaggtc
    11041 ggtgaggtgg cggatgtgtt gtgcggcaaa ttgttttata tcactatcag acggtttaag
    11101 aagattggta aagttagtgt tatgttttga gtttggattc atgagagaag gttttcagac
    11161 gacctttgtc tgatacggga tgaaacgggc aaaggtcgtc tgaaaaatga taggttgaaa
    11221 acagctgaat tttacccgaa aaaaagcgga tatgccgtaa cgacatatcc gctttgattg
    11281 cattcgattt taggagaaca ggcgcaatgc ggttttgccg cccggttcga taccgacttt
    11341 gagcatctcg gactgacgag ccaatacata agtgcgtacg tctttgagcc attgggtcga
    11401 aacttcttcc attttgtcgt tgaccagatt caggttcaac tggccggaca ggcgtaccgc
    11461 caaatccata aacaaatcgt cgaaggtttt ttcgcctgcc ggagagtgcg ggatgtcgag
    11521 cagcatactg aagcctttgt aggactggtt gtccaaaagg gcattggtaa acggctcgtt
    11581 gttgagcgag cagatggaga acatggttga gcccgacgtg tcggtatagt ggaacgcgcc
    11641 gtcgtcttcc aaaacgaaac ccacgcccgt tacggcggaa cgcagttcta cgccgctgat
    11701 gctggtcggg gaaaccaaat ggatggcgat ggtctggtcg acgcgcgcgc agaatgcgtc
    11761 cagtgcggaa gccacttcga taaaggcggc aaggtcggtg tgcagcgtct gaccgcccat
    11821 gctttgtgcg aatgcgtcca cctggcggtt gaatgcggag agttcttcct gcgaggcaag
    11881 tccgttgcgg ctgactgcct gaatacccac gataaatgcc tgatagcgga tgcccgggat
    11941 gggttcggca atctggaaat ggtcgtccat ggtgcagccg acaatctggt agcggcagcg
    12001 gttggaaagg cgcggcagtg cgtgcagttc tttggcttcg gtcagcgcga tataggagat
    12061 gaagtcgaag cgcacgtcaa accagggtaa ttcgactttt gacagttctt tgagcgtaat
    12121 cagcggtttt gcaggtgttt gcggaacggg tgcaggtttt gccggcgcgt cggcaggttt
    12181 cggtgcggaa tgtccggttt ggggttcgga aacggtgtgg gcggagttgc cgataatgcc
    12241 gctttcttcc aaggcggttt cgatttcggt tttgaacggg gaggcttttg cctgtttctg
    12301 cttggcgatg tagacggcat cctgttcttg caggttgcgc atggcggggt cttggggttt
    12361 tgccgttttt ttgaccgccg gttggggttt cggcatcatg actggcccgc cggacggttg
    12421 gccgtcgcgg acatggctgg ttttgctgtt gagcagggca tctttgtcgg agtgtccgaa
    12481 ctggtcgcgc acttttttgc ggtattggtt ttcctgatac atattgtagg cgacaacggc
    12541 gaggacggcg gcgaggaaca gtacgatgta aatcatggca atcactttaa atttcgggat
    12601 gcaggatgcg cgaagtgcgg gtactgcggt taaatcgggc ttgcgtttcc ggcagtctga
    12661 tggaacggcc ggttataacg tttgaattat aacgaaaatt gcagggtctg acagcagtgt
    12721 gtcgaaataa gcggaaattt tccgaaatgc cgtctaaaat ctgtggtttt cagacggcat
    12781 ttctgtctac gggaaaccct ttctcccgta tccgccgcca gtcgaaaaag tggccggggt
    12841 cggttttgcg gccgggcgcg atgtcttggt gccccgttac cgccgtgatg gggtagcggc
    12901 ggcagattgc gtccaacaag gcttcgagcg aacggtattg cgcttcggta aagggttcga
    12961 aatcgcagcc ttccagttcg atgccgatgg aaaatgcatt gcatttttcc ctgccgccga
    13021 atgaagacac gccggcgtgg tatgccatat tgtcgcagga aacgaactgt accgtttctc
    13081 cgccgcgttt gattaaaaaa tggctggata cgcgcaaagt gtgtatcagg ctgaagaacg
    13141 gatgtccgtc ggggtcgagc cggttggcaa acagcttttc caccgcatcc gtaccatatt
    13201 cgaacggcgg cagcgagata ttgtgcaata cgacaaggga aacctcttct ttggactccc
    13261 tcgggccgaa attcggcgac ggaatgtggc gcacgctttg cagccaaccg tcctgccagt
    13321 gtgcttcggc gtgattgtcc atgatgttct tcctgtccgg cgggtaattt gggttatact
    13381 gtcgcccgaa ttttaagacg tattccgaat gctgggaatc ctaccatgtt gagaaaattg
    13441 ttgaaatggt ctgccgtttt tttgaccgtg tcggcagccg ttttcgccgc gctgcttttt
    13501 gttcctaagg ataatggcag ggcataccga atcaagattg ccaaaaatca gggtatttcg
    13561 tcggtcggca ggaaacttgc cgaagaccgc atcgtgttca gcaggcatgt tttgacggcg
    13621 gcggcctacg ttttgggtgt gcacaacagg ctgcatacgg ggacgtacag actgccttcg
    13681 gaagtgtctg cttgggatat cttgcagaaa atgcgcggcg gcaggccgga ttccgttacc
    13741 gtgcagatta tcgaaggttc gcgtttttcg catatgagga aagtcatcga cgcaacgccc
    13801 gacatcgaac acgacaccaa aggctggagc aatgaaaaac tgatggcgga agttgcccct
    13861 gatgccttca gcggcaatcc tgaagggcag tttttccccg acagctacga aatcgatgcg
    13921 ggcggcagcg atttacggat ttaccaaacc gcctacaagg cgatgcaacg ccgcctgaat
    13981 gaggcatggg aaagcaggca ggacgggctg ccttataaaa atccttatga aatgctgatt
    14041 atggcgagcc tggtcgaaaa ggaaacaggg catgaagccg accgcgacca tgtcgcttcc
    14101 gtcttcgtca accggctgaa aatcggtatg cgcctgcaaa ccgacccgtc cgtgatttac
    14161 ggcatgggtg cggcatacaa gggcaaaatc cgtaaagccg acctgcgccg cgacacgccg
    14221 tacaacacct acacgcgcgg cggtctgccg ccaaccccga ttgcgctgcc cggcaaggcg
    14281 gcactcgatg ccgccgccca tccgtccggc gaaaaatacc tgtatttcgt gtccaaaatg
    14341 gacggcacgg gcttgagcca gttcagccat gatttgaccg aacacaatgc cgccgtccgc
    14401 aaatatattt tgaaaaaata aaccatgccg tctgaaaagt ttgtgttttc ggacggcata
    14461 cccttaccgg aactgcaagc atgaaaccgc aattcatcac tttggacggc atagacggtg
    14521 ccggcaaatc caccaacctt gccgtcatca aggcatggtt tgaacggagg gggctgcccg
    14581 tgctgttcac gcgcgagccg ggcggaacgc cggtcggtga ggccttgcgc gaaatcctgc
    14641 tcaaccctga aaccaaagcc ggtttgcgtg cggaaacact gatgatgttc gccgccagaa
    14701 tgcagcatat tgaagacgtg atattgcctg cactttcaga cggcatccat gtcgtgtccg
    14761 accgttttac cgatgcaacc ttcgcctatc agggcggcgg gcggggaatg ccgtctgaag
    14821 acattgaaat tttggaacat tgggtgcagg gcggtttgcg ccccgatttg accctgctgt
    14881 tggatgtgcc gctggaagtg tcgatggcgc gtatcgggca gacgcgcgag aaagaccggt
    14941 tcgagcagga acaggcagat ttcttcatgc gtgtgcgcag cgtttatctg aaccgtgccg
    15001 ccgcctgtcc cgaacggtac gccattatcg acagtaaccg cagcttggat gaagtcagaa
    15061 acagcataga aaaagtgttg gacggacatt tcggctgttg atgcggcaaa tattgaaaca
    15121 agcgcatccg cccgcgccga aaatcaaacg gcagtgccac aggtgaaaat ggcggtatgc
    15181 gccaaacttt cggcatgata gaattacgct tagttacaag gcaggatgcg tcggcaatat
    15241 taacgaaccg cccgtaacat gatgacccga aagcgtttcg gacagtccga ttcaaatctt
    15301 tttctcgcaa caggattgac acatggaaaa ctcattgaaa gaagccgccc tcaagttcca
    15361 cgaattaccc gttccgggca aaatttccgt taccccgacc aaatctctgg cgaccgacaa
    15421 agatttggcg ttggcgtact ctccgggcgt agccgctcct tgtatggaaa tccatgccga
    15481 tccgcaaaat gcctacaaat acaccgccaa aggcaacttg gtcgccgtca tttccaacgg
    15541 taccgcagtg ttgggtttgg gcaacatcgg cgcattggcc ggtaaacctg taatggaagg
    15601 caaaggcgta ttgttcaaaa aattcgccgg cgttgacgta ttcgatatcg aaatcgatga
    15661 aaaagacccg caaaaattgg tagacatcat cgcttctttg gaaccgactt tcggcggcat
    15721 caacctcgaa gacatcaaag cgcctgagtg cttctacatc gaacgcgaat tgcgcaaacg
    15781 ttgcaaaatc cctgtattcc acgacgacca acacggcacg gccatcatta ccgctgccgc
    15841 cgtattgaac gccttgcgtt ataccggccg caaaattgaa gaagcaactt tggtgtgctc
    15901 cggtgcaggt gctgcagcga ttgcttgctt gaaccaattg ctggatttgg gtctgaaacg
    15961 tgaaaacgta accgtctgcg actcaaaagg cgtgatttac aaaacccgcg aagacaaaga
    16021 tcgcatggat gagtctaaac agttctacgc cattgaagac aacggccagc gcgtattggc
    16081 cgatgcggtt aaaggcaaag acatcttctt gggtctgtcc ggcgcgaacc tgctgacccc
    16141 cgaaatgttg aacacgatga acgaaaaacc catcgtgttc gctatggcca acccgaatcc
    16201 ggaaatcctg ccgccgctgg caaaagaaac ccgtccggac gtggttatcg gtaccggccg
    16261 ctcagatttc ccgaatcaag taaacaacgt attgtgcttc ccattcatct tccgtggcgc
    16321 gttggatgtc ggcgcgacca ccatcaacga agaaatgaaa cgcgcctgcg tgtatgcttt
    16381 ggcggatttg gcgatggaag aagtgaccga agaagtggtt gccgcttacg gtaagaaatt
    16441 tgaattcggc gcggaatacc tgattccgac tccgttcgat tcccgcctgc tgccgcgcgt
    16501 tgctacggct gccgccaaag cagcgatgga aagcggcgtg gcaacccgtc cgattgcaga
    16561 tttggaagct tacgctgcca agctgagcga atggaagctg taagccgttt gcggtttaaa
    16621 atgccgtctg aactgttttc aggcggtatt ttgctgtcag attgatatag tggattaaca
    16681 aaaatcagga caaggcgacg aagctgcaga cagtacaaat agtacggaac cgatttactt
    16741 ggtgcttgag caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgctgtactg
    16801 gtttttgtta atccactata aatgaaagat actggaaaat gaaagagatg aaacctgtcc
    16861 gttatcatat tggcgatatg cccgagactt caaaacaaac cgcctcccgt catgacgaca
    16921 gggcagtggg tgttgacgat gattgttttc atgattcctt tggtcaattt ttgtttgggt
    16981 gttcggcaga ggcaacccga accgcgccaa tttctgtaaa gcgcagttgc ttatttacct
    17041 gattggttcg cttatcggtt tggtcttcgc gttgtttata ggtgggtctg tatcgggtac
    17101 gcatgattaa tgccccgggc tgattttgct tcgaggattt gtatcgaata tgccgaattg
    17161 tttcaaattt cataccgtta tcgaacggca ttggcaaaaa ccttatccgg ttttgtcttt
    17221 tctgcttaag ccgctctccg ggctgtttgc caaaattgcg gcaaaacggc ggacggattt
    17281 tttatcggga aaacggcaaa gcgaaaagct gcccgtgcct gtggtcgtgg tcggcaatat
    17341 tcacgcgggt gggacgggga aaacgccgat tgttgccgcg ctggtgtcgg gtttgcagga
    17401 aaagggcgtc aaggtcggca tcatcagccg gggctacggg cgcaagagca aggcggttca
    17461 tgtattgaat gctgagagcc gagcggaaga tgcgggcgat gagcctttgc tgctgttccg
    17521 caaaaccggt gcgccgacgg cggtgggcag cagccgtgca gaggcaggca gggcgttgct
    17581 ggcggcgcat cccgacatcg gactgattgt ggcggacgac ggtttgcagc attacgccct
    17641 gcggcgagat gtggaaatcg cggtgtttcc gtcggcggat acggggcgca cggatttgga
    17701 tttactgccc aacggcagtt tgcgcgaacc tttgttgcgg ctggattcgg tggatgcggt
    17761 cgtcgtcagc ggcggcaagg cggatgcgct gtttaggccg tctgaaaata tgtttcacag
    17821 ccgtatcgaa gcgggacgga tttaccgttt gaacaatccg tccgaaatac tggacacagg
    17881 ccgtctgaaa aatcaaaccg tcgtcgccgt ggcaggtatt gccaagccgg cgcgtttttt
    17941 cgattcgttg cggaatatgg gcattaccgt gaagcgaacc gtcgcgctgc ctgaccacgc
    18001 cgacatttcg gcggcagatt tgcccgatgc ggacgcggtc attattacgg agaaagatgc
    18061 ggtcaaattt tcagacggca tttgcaccga taatgtttgg gtgttgcccg tttgtgcgat
    18121 aatcgaacct gatttggcgg cgtttgtgtt ggagcggttg gaagatgtac cgaaggccgt
    18181 ctgaaagcac ggtttgggcg gagtgattac ggatttgaat aagaacgcct cgcgccgtca
    18241 ttcccgcgca ggcgggaatc taagtctcga attttcagga atgcctagga ggcttcagaa
    18301 atcccaaatc tccggatttc cacttggaca ggaatgagaa aatcggtcgt attttttatc
    18361 tgcattaatc attcgttaaa gtattgaata ttaaactgaa aaccttgtta ttgcccttcg
    18421 ccacgctggc attgtgcacc aacgcttttg ccgccccgcc cagcgacgcg tcgttggcgc
    18481 gttggctgga tacgcagaat tttgaccggg atatagaaaa aaatatgatt gagggcttta
    18541 atgccggatt taaaccgtat gcggacaaag cccttgccga aatgccggaa gcgaaaaaag
    18601 atcaggcggc aaaagccttt aaccgctatc gtgagaatgt tttgaaagat ttgattacgc
    18661 ccgaagtgaa acaggctgtc cgcaatactt tattgaagaa tgcccgtgag atatacacgc
    18721 aagaagaaat tgacggcatg attgcctttt acggttcgcc tgtcggtcag tccgtcgttg
    18781 ccaaaaatcc gcgcttaatc aagaaatcga tgagtgaaat agcagtatct tggactgcat
    18841 tgtcagggaa aatcgcgcga catcatctgc ccgcgtttac ggaagagttg cggcgcatca
    18901 tctgcggcgg taaaaatccc gatgcgggct gtaaacaagc cggacaggtt gggaaaaggc
    18961 atcagaaata aatgatagcc gtctgaaata ttgaagaggg catccgattg attgaaccat
    19021 caaacccgaa agcaacccta tggaaaaaaa attcttagac atcctcgtct gccccgttac
    19081 caaaggcagg ctggaatatc atcaggacaa acaggaattg tggagccgtc aggcgaagct
    19141 tgcctatccg attaaagacg gcattcccta tatgctggaa aacgaagcgc gtccgttggg
    19201 cgaagaggaa ctcgaagcat gaccgaattc gtcgtattga ttccggcgcg gctggattcg
    19261 tcgcgcctgc ccggaaaagc cttggcggac atccacggca aaccgatggt cgtgcgcgtt
    19321 gccgaacagg cggcaaaaag taaagctgtg cgcgtcgtcg ttgccaccga ccatcccgat
    19381 attcagacgg cctgtcaggc gcacggcgtc gaagtcgtca tgacttcaaa ccggcacgaa
    19441 agcggcacga cgcgccttgc cgaagcctct gccgcgctga agctgccgcc gcatctggtg
    19501 gttgtcaacg tacagggcga cgagccgctg attgcacctg aactcatcga ccgcaccgcc
    19561 gaagtactcg tcgaaaacaa cgtccaaatg gcgaccgccg cccacgaatt gcacgatttc
    19621 gacgaattga tgaatcccaa tgccgtcaaa gttgtcctcg acaaaaaccg caacgccatc
    19681 tacttcagcc gcgccccgat tccctatccg cgcgatgcga tgcgtgcagg aaaacgcgaa
    19741 atgccgtttg aaaccgccgt cctgcgccat atcggcatct acgcctaccg cgccggcttc
    19801 ctgcaacgct atgccgaaat gagcgtttcg ccgctggaaa ccatcgaatc gctggaacag
    19861 ctgcgcgtcc tgtggcacgg ctacccgatt gccgtcgaaa ccgccaaaga agcccccgcc
    19921 gccggtgtgg atacgcagga agatttggat agggttcgcg ctgtatttca gactatataa
    19981 aatagattca aagggaaaag atatgcagca acatattgaa aagtggcaat acttgagccg
    20041 ggaagaacag aaaatccttg ccgaagtatg gggtctcgtg caaaacgatg atcaggaggt
    20101 tcactatgaa atgcttaaat tgaacgcacc cgacgaagcc agtggcgaat tttggttcag
    20161 aatggcagaa acactcagca ccctgccgcc caaccgttcc ctcgacctta gaatgaacgg
    20221 cggcaggctg gcgaccgccg tatccatcct ttccgtcatg attgaagaca atcccgacat
    20281 accgcagctt tgggcgcaaa aaattaccgc cctcaattat agtggattaa atttaaacca
    20341 gtacggcgtt gcctcgcctt gtcgtactat ctgtactgtc tgcggcttcg tcgccttgtc
    20401 ctgatttttg ttaatccact atatttggca cacggacaca aagcccgtgc cgacggtttg
    20461 gcacaacagc ccgacaaagc ggcagaagcc aacgaggagg aatacctgac caaagccctg
    20521 tcgcaaaacc tgctgtcaac attggatgtc gcgcttgcac gttttcctga agacgcgtgg
    20581 tttcaggaaa tcaaacagga tgcacaaaag cattttgctt gaggatgtgg cagtcaggaa
    20641 tatttccatt caggaagaaa agaagtgcct gattgggtat aatcagggta aatcttattt
    20701 tatttcaaaa gattaatatt tgctttccat ttttccttga cggtatcgga aaagttgatt
    20761 atagttacag cttccttagg agtaatggct gagaggctga aggcacttcc ctgctaagga
    20821 agcatgtggg gtcaacctgc atcgagggtt cgaatccctc ttactccgcc agataaaaaa
    20881 tagacgctgt gttttacagc gtctattttt ttatgcaatt ttatagcggg ttggtgcaaa
    20941 accagtatgg tattgccctg tcttgattct gaattttgtt atagtggatt aacaaaaacc
    21001 agtacggcgt tgcctcgcct tagctcaaag agaacgattc tctaaggtgc tggagcacca
    21061 agtgaatcgg ctccgtacta tttgtactgt ctgcggcttc gccgccttgt cctgattttt
    21121 gttaatccac tataatccga gatgcttgcc gtttatttcc gcctcgttca aacggcggct
    21181 ctgatttgcg tggtttctgt ttgccgtatt cgcctatccg taccgcaaat gttatactgg
    21241 gaaaaattta ctgattgtgt tttatggcat atttgccgat aggatggaag agacaaatga
    21301 gcagaatccg gcaggctttt gccgctttgg atggcggaaa ggcattgatt ccctatatta
    21361 cggtgggcga ccccgatatt cggacaactt tggcattgat gcacggcatg gttgcaaacg
    21421 gtgcggatat tttggagttg ggcgtgccgt tttccgatcc gatggcggat gggccggtta
    21481 ttcagcgtgc ggcggagcgg gcgttggcaa acgggatttc gctgcgcgat gtcttggatg
    21541 tcgtcagaaa attccgtgaa accgacacgc aaacgccggt tgttttgatg ggatatttga
    21601 accctgtaca taagatgggt tatcgggagt ttgctcagga agccgcaaag gcgggtgtgg
    21661 acggcgtgtt gacggtggat tcccctgtcg aaaccatcga tccgctctat cgcgagctga
    21721 aggataacgg ggtcgactgt attttcctga ttgcgccgac gacgacggaa gaccgtatta
    21781 aaaccattgc cgagctggca ggcggatttg tctattatgt ttcgctcaag ggcgtaacgg
    21841 gcgcggcaag tttggatacg gatgaggttt cgcgtaaaat agagtatttg catcagtata
    21901 tcgatattcc catcggtgtc ggtttcggca tcagcaatgc ggaaagtgca cgcaaaatcg
    21961 gccgggttgc cgacgcagtt attgtcggca gccggattgt gaaagaaatc gaaaacaata
    22021 caggcaacga ggctgccgcc gtcggtgctt tggtaaaaga gttgaaggat gccgtgcgct
    22081 gacggcggtt cctcatcctg aatattttag gagttgtcca tgagctggtt agataaaatc
    22141 ctgccaccca aaatcaagaa tcgcggaaaa gacggttctt ccaatgttcc cgagggtcta
    22201 tggcacaaat gcccgtcttg ttcggcaacc gtttattcta ccgagttgca gcagaacaat
    22261 caggtttgtc cgaaatgcaa ccaccacaat ccgttgtcgg cacgacaacg cctgaatctg
    22321 cttttggatg aggatggcag agaggagatt gccggaaatg tcaaaccgac cgatcctttg
    22381 aagtttaaag acagcaaaaa atatccggat cgtttgagtg cggcacgcaa gctgaccggg
    22441 gaagatgatg ctttggtggt aatgaaaggc aagatgaacg gtctgcccgt cgtcgttgcc
    22501 gcgtttgaat tccgctttat cggcggttcg atgggttcgg ttgtgggcga acgattcgta
    22561 caaggtatcc gtcgggcggt tgccgacaat tgtccgttcg tctgtgtggc ggcttccggc
    22621 ggtgcgcgta tgcaggaggg tgtgaactca ttgatgcaga tgacgaaaac cagtgccgcg
    22681 ctgcatttgc tgacggaaaa acgcctgcca tttatatcgg tgttgaccga tccgactatg
    22741 ggcggcgtat ccgccagctt cgcatttttg ggcgatgtcg tgcttgccga accgaacgcg
    22801 ctgatcggtt ttgccggtcc gcgcgtgatt gagcagacgg tgcgcgaaac gctgccggaa
    22861 agcttccaac gcgccgagtt cctgctggaa aaaggcgcaa tcgaccagat tgtcgaccgc
    22921 cgcgatatga agcggcgcat cagtgatttg attacgctgt tgtgccgtca ggacaaagtt
    22981 tccgccgctt gatggctgat gaatcgaata ccgtctgaaa ccgatgtttc agacggtatt
    23041 tttgtgtctg gttatttgtt gtgcggcttt atcgacgggg catagcgtcc ggcacgttct
    23101 ttcaggcgtt gtaccaaacc tttcgtgtcg gcgggtacac cgccctcgca gaatgcctga
    23161 tacaggacgg tgcgcagtgc gtcgttgcgg cttaatgtac cgcctatcgg tttccattcg
    23221 gcgtttcggg gctgtatcca gcggcggttg accgtgtcgc cgtagccgaa cactttatag
    23281 gaggaaggtt tgccgttgcc gaacctgatg gagaagcggg cgcagaatat gccttcggca
    23341 gtcaggttgt cgtagccttt gtcggagcgg atattgagaa tgtagcggat gctgccgtcg
    23401 ggcgcgggca taatttgcag gctgtcgagc aggattttcg gctgtttgcc gtaattttca
    23461 tccacataaa tgtcgaacca gccgtccgag tgcgtatcgg gcagcggcgg cagtttggcg
    23521 gtatgttctt taaattcgcg ggcggcggcc tcttcgggcg tttcgcggta gcgggtgttg
    23581 atcggcgtgt ctttttggct gaagccggca gcgagggacg tgccggcggc gagtgcgagc
    23641 agcaggaggg cggtgcggcg cataagtttc tccaaattga aaacggcgtt attttatggg
    23701 ttggcaaagg gggctgcaag caactggggt ataatctccc cccgattccc attttttaaa
    23761 cggtacaaac gatgaacagc gaaactttag acgtaaccgg attgaaatgt cccctgccga
    23821 ttttgcgggc gaaaaaggct ttggcgcaaa tgcagcaggg cgacgtgttg accgttctgg
    23881 caaccgacgg cggcgcaccg ggggattttg aggctttttg ccgccaaacc ggtcatgtgc
    23941 tgttggatgc ttccgaacag gacggcgtgt tcacgctggt cgtccaacac aaataaatgc
    24001 cgtctgaaat gcggatgtcc cgattgatgt tattgctgtc ggcatttgtg cttgctgcgt
    24061 gtaatgccca aaaccggcaa tccggtgtac ggcatcagga tttcgcgcgt gtagccgtcg
    24121 atttgtttcg gagtgcctgc gtcctgactc aaggcagttt tgaatcggtt tccaagtttg
    24181 ccgctgtcgg acattttgtc cctgtcggaa aggaagacct gtcccatctg ccgcccactg
    24241 ttgcagaacc cgatgtacag gcgttgtgga cgcttgaacg cgatggcggc atttattatt
    24301 tgagcctgac gcgcgacagt tgcagtgtga aagccgagcg ggcagacagt gccgcgctgt
    24361 tggaacattt tgacggactt gtccgacagc cgccgaaaaa tgccaatata gaattgaggg
    24421 cggaacagtc ggtgcaggca tcatttgaaa tccgccaagt cagctatgcc tggcgggaag
    24481 cgggcagctc cgaagaaacc gtattgaccg cacaaaccgc cgattccccg gatttacccg
    24541 tacaggcagt tttgaacctg acgcaccgtt cccacaacgg gaaaccccta attctcccct
    24601 aacttcagac ggcataaagt cggcatgccg tctgaacatt caaataaaac aaaggaaaac
    24661 catgcaaacc ctgaccatta tccgccccga cgatatgcac ctgcacctgc gcgacggcga
    24721 cgcgctcaaa gccgttgctc cttataccgc ccgccaaatg gggcgtgccg tcattatgcc
    24781 caacctcaaa ccgcctgtcg tcagcgttgc cgacgcgctt gcctacaaag cgcgcatcat
    24841 ggcggcattg cccgaaggca gcgcgtttga gccgttgatg acgctttacc tgaccgacca
    24901 agccacgcct gcacttgtgc gcgaagccaa agccgccggc atcgtcgcct tcaaactcta
    24961 ccctgccggc gcgaccacca attccgattc cggcgtaacc gacctgttca agctcatccc
    25021 cgtgttggaa gaaatggcga aacagggcat tttgttcctc gttcacggcg aagtaaccga
    25081 ccccgaaatc gacatcttcg accgcgaagc cgcctttatc gggcgcgtga tgaaacccgt
    25141 tttggcgcaa gtgccgaatc ttaaagtcgt gttcgaacac atcaccaccg ccgaagccgc
    25201 ccgcctggtt ttggaagcag gcgacaacgt agccgccacc gttaccccgc aacacctcct
    25261 gctcaaccgc aacgacctct tggtcggcgg cgtgcgcccc catcatttct gcctgcccgt
    25321 actcaaacgc gaaacccacc gtcaggcatt ggttgccgcc gttaccggcg agaaggcgca
    25381 taaattcttc ctcggcaccg actccgcgcc gcacgccaaa tccgccaaag aaaacgcctg
    25441 cggttgcgcc ggtatgttca gcgcgatgac cgccatcgag ctttatgccg aagtgtttga
    25501 aaaagcaggc gcgttggaca aactcgaagc cttcgcctcg aaaaacggcg caaggtttta
    25561 cggcattcct gaaaataccg acacgatcac cctcgtcaaa caaagccaaa ccgtccccgc
    25621 aagtattccc tacggcgacg gcgaacttgt cccgatgcgc gcgggcggcg aaatcggctg
    25681 gacggtacag tattgatggg gctggaaaca aaatgccgtc tgaaaggttt ttcagacggc
    25741 atttgtgttg ttgactgatt gatacgtcaa cggcgtttga gtttgttacg tttcggttat
    25801 ttccgataaa ttcccacaat tttcaaattt cgccattccc acgaaggcag gaatccagaa
    25861 attcgatgcg accaaagttt atcaaaaacg gcagcaactc aaaaaaccgg attcccgcct
    25921 gcgcgggaat gacgagattg aagtttcaga atttatttga aatacccaaa attcaaaaaa
    25981 ccaaattccc acctgcgtgg gaatgacgaa acaaagaaag cagaaataag gacatagaac
    26041 tttctttaaa tttgtgatgc atcaacggcg tttgggctcg tcggggcgga tttgggcggc
    26101 gagtttgtcg aggatgccgt tgacgaattt gtgcccgtcc gtgccgccga aggttttggt
    26161 aacttcgatg gcttcgttga tgatgacggg gtagggtgtt tcgggcatgg cggacagctc
    26221 gtggcaggcg gtcagcagga cggcgcgttc gatgggattg aggtcttttt cgtcgcggtc
    26281 gagcaggggg cggatttttt ggatgtagtc cgctgcgttg gtttgcgtac cgaagaaaag
    26341 tttgttgaac aattcttcgt ccgctttggc aaagtcgggc atttcgcgga tgtttttagc
    26401 aatttcgggc gcggcggtgc ggttgataag ggattggtaa acggcttgta cggcaagctc
    26461 gcgggaacgg cggcgggctg ttttcatgat ttttccttga aacggttggg cggcacggta
    26521 tgccgtctga aacggaaagg gcgtattggt gtacgccctg tttgttattc ttcgtcttca
    26581 aactgttctt cgagcagcag gttgacgagg ttggcgcatt cgacggcgac tttggcggca
    26641 tccgaggctt tttcttcaat ccgttcgatt gcctgcgcgt cgttttcggt ggttaggacg
    26701 gcattggcaa tcgggatgtt gtagtcgagt gcgacgcggc taacccctgc tccggattcg
    26761 ttggaaacca gctcgaaatg gtaggtttcg ccacggatga cgacaccgat ggcaatcagt
    26821 gcgtcaaact tttcggaaga ggcaaggttc atcagtgcga tggggatttc aagtgcgccg
    26881 ggtacggtgg cgacggtaat gttttcgtct gccacgccca attcttggag ggtgcggcag
    26941 cagactttga gcatttcgct gccgatttcg ttggtgaaac gtgcctgtac gatgccgatg
    27001 cggaggtgtt tgccgtcgag gttgggggcg atggtgttca ttgggtgtcc tttggtattc
    27061 ggaggtttcg gaatgccgtc tgaaggtttc agtcttgcgg ctgccagtcg gcgacggttt
    27121 ggaatgtgcc gtcttcggca agctcccatg cgctgccttc gggttgggaa agcagtgcgg
    27181 cggtttcagg gttggttttg gcgatgtcgg cgaggctgac gatgctgaag ttgtcgggat
    27241 cgtcggtgta ttcgtcggtc tcgtcgccgc tgaagaaacg ccagccgctg tcgttttcaa
    27301 aaacgggggc ttcgcggtag aggaagccga cgggccggtt ttgtttggcg acggtgttgg
    27361 tggcgataca gcggtcgagt gccgaggaaa gtgcttgtgc aaatgcgttc attacgggaa
    27421 tacgttgggg gaaaacttac ggattttacc acgattcgtg cgttgtcggc agacggcggc
    27481 ggtttggtgg tacaatgtgc gccgtttgca gccttaaggt gtttctgtat ttttggagta
    27541 tggaaacgca ttcgggctgt tttttgcgga agacggtaat gaaagacgat gttttgaaac
    27601 agcaggcaca cgcggcgata cagaagagac tgggctacgc gttccgcgat atttcgcttt
    27661 tgcggcaggc tttgacgcac aggagccatc atgcgaagca caacgagcgg ttcgagtttg
    27721 tcggtgattc gattttgaat tatacggtgg cgcggatgct gtttgacacg tttccgaagt
    27781 tgaccgaggg cgagttgtcg cggttgcggg caagtctggt caatgagggc gtgctggcgg
    27841 aaatggcggc ggaaatgaat gtcggcgacg gtctgtactt gggggcgggc gagttgaaga
    27901 gcggcggctt cagacggcct tcgatactgg cggacgcgat ggaggcgatg tttgcggcgg
    27961 tcagcttcga tgccgatttc aacacggcgg aaaaggtggt gcgccatttg tttgccgacc
    28021 gcgtccggtg tgccgatttc caaaatcagg caaaagacgg caaaactgct ttgcaggagg
    28081 cgttgcaggc acgccgcttt gctttgccga aataccgtat cgaagagcaa atcggccatg
    28141 ccaacgacag tatgtttgtg gtttcctgcg atttgggcga actgggtttc gtgtgccgtg
    28201 ccaaagggac gagccgcaag gcggcggaac aggaggcggc gaaagaggct ttgaaatggc
    28261 tggaagagaa gctgccgctg aagaagaaaa agaaatgagg cggcgcgtga atatgccgtc
    28321 tgaaatgtgg atatgaaagc gaatatggat attgaaacct tccttgcagg ggaacgcgcc
    28381 gccggcggat accgttgcgg cttcgtggcg attgtcggtc gtccgaacgt gggcaaatca
    28441 acgctgatga accatctcat cggtcagaaa atcagcatca ccagcaaaaa ggcgcagacg
    28501 acgcgcaacc gcgtaacggg gatttatacc gacgataccg cgcagttcgt gtttgtcgat
    28561 acgcccggct ttcaaaccga ccaccgcaac gcgctcaacg accgtttgaa tcaaaacgtt
    28621 accgaggcac tcggcggcgt ggatgtggtg gttttcgtcg tggaagcgat gcgctttacc
    28681 gatgccgacc gtgtcgtgtt gaaacaactg cccaagcaca cgccggtcat tttagtggtc
    28741 aacaaaatcg acaaggacaa ggcgaaagac cgttacgcgc tggaggcgtt tgttgcccaa
    28801 gtgcgcgccg aatttgaatt tgcggcggcg gaggcggtca gtgcgaaaca cggattgcgg
    28861 attgccaacc tgttggagct gctcaagccg tatctgcccg aaagcgtgcc gatgtatccc
    28921 gaagatatgg ttacggacaa atcggcgcgt tttttagcga tggaaatcgt gcgtgaaaaa
    28981 ttgttccgct atttgggcga ggaattgcct tatgcgatga acgtcgaagt ggagcagttt
    29041 gaagaggaag acggtttgaa ccgcatctat atcgccgttt tggtcgataa ggaaagccaa
    29101 aaggcgattt taatcggcaa aggcggggag cgtttgaaga aaatttccac cgaagcgcgg
    29161 ttggatatgg aaaaactgtt tgataccaaa gtatttttga aggtctgggt caaagtcaaa
    29221 tccggttggg cggacgacat ccgcttcctg cgcgagctgg gtttgtagtt tttcttgctg
    29281 aactttacgc aaatgccgtc cgaacaggtt tcagacggca ttttgtttca atcgggaata
    29341 tctttgttaa aaacgggttg atattatctg tgcata
//
LOCUS       DAWFJR010000018        28000 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWFJR010000018 DAWFJR010000000
VERSION     DAWFJR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28000
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.018"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            38..461
                     /locus_tag="WGR04_000706"
                     /pseudo
     CDS             38..461
                     /locus_tag="WGR04_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            567..1130
                     /gene="pgsA"
                     /locus_tag="WGR04_000707"
     CDS             567..1130
                     /gene="pgsA"
                     /locus_tag="WGR04_000707"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ0794309.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NILMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1210..1285
                     /locus_tag="WGR04_000708"
     tRNA            1210..1285
                     /locus_tag="WGR04_000708"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1243..1245,aa:Gly,seq:gcc)
     gene            1311..1386
                     /locus_tag="WGR04_000709"
     tRNA            1311..1386
                     /locus_tag="WGR04_000709"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Gly,seq:gcc)
     gene            1410..1485
                     /locus_tag="WGR04_000710"
     tRNA            1410..1485
                     /locus_tag="WGR04_000710"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1443..1445,aa:Gly,seq:gcc)
     gene            1507..1582
                     /locus_tag="WGR04_000711"
     tRNA            1507..1582
                     /locus_tag="WGR04_000711"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1540..1542,aa:Gly,seq:gcc)
     gene            1607..1680
                     /locus_tag="WGR04_000712"
     tRNA            1607..1680
                     /locus_tag="WGR04_000712"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1639..1641,aa:Cys,seq:gca)
     gene            1783..1872
                     /locus_tag="WGR04_000713"
     tRNA            1783..1872
                     /locus_tag="WGR04_000713"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1817..1819,aa:Leu,seq:taa)
     gene            1976..3028
                     /gene="sohB"
                     /locus_tag="WGR04_000714"
     CDS             1976..3028
                     /gene="sohB"
                     /locus_tag="WGR04_000714"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ0794310.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3079..4004)
                     /locus_tag="WGR04_000715"
                     /pseudo
     CDS             complement(3079..4004)
                     /locus_tag="WGR04_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201178.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
     gene            complement(4058..4489)
                     /locus_tag="WGR04_000716"
     CDS             complement(4058..4489)
                     /locus_tag="WGR04_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ0794311.1"
                     /translation="MNRLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQEGEQDRRKRLLSLTETGKAYAAPLTE
                     SAQEFSDKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            complement(4614..5483)
                     /locus_tag="WGR04_000717"
     CDS             complement(4614..5483)
                     /locus_tag="WGR04_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ0794312.1"
                     /translation="MSANEYAQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGK
                     QDVSGVYLKLAEH"
     gene            complement(5573..6490)
                     /gene="hisB"
                     /locus_tag="WGR04_000718"
     CDS             complement(5573..6490)
                     /gene="hisB"
                     /locus_tag="WGR04_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ0794313.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLPKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(6487..7584)
                     /locus_tag="WGR04_000719"
     CDS             complement(6487..7584)
                     /locus_tag="WGR04_000719"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ0794314.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            complement(7630..8919)
                     /gene="hisD"
                     /locus_tag="WGR04_000720"
     CDS             complement(7630..8919)
                     /gene="hisD"
                     /locus_tag="WGR04_000720"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ0794315.1"
                     /translation="MKKLNTQSPDFQTGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKLDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVITDGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMNRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(8916..9815)
                     /locus_tag="WGR04_000721"
     CDS             complement(8916..9815)
                     /locus_tag="WGR04_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ0794316.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            complement(9919..10572)
                     /locus_tag="WGR04_000722"
     CDS             complement(9919..10572)
                     /locus_tag="WGR04_000722"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ0794317.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            complement(10651..11304)
                     /locus_tag="WGR04_000723"
     CDS             complement(10651..11304)
                     /locus_tag="WGR04_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ0794318.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFNLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            11581..11665
                     /locus_tag="WGR04_000724"
     tRNA            11581..11665
                     /locus_tag="WGR04_000724"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11615..11617,aa:Leu,seq:caa)
     gene            12424..14151
                     /gene="ilvB"
                     /locus_tag="WGR04_000725"
     CDS             12424..14151
                     /gene="ilvB"
                     /locus_tag="WGR04_000725"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ0794319.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKVDIPIVGDVKNVLSEMVALW
                     QKQEFMPSEDALGKWWKTIEEWRSQDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADEKA"
     gene            14162..14653
                     /gene="ilvN"
                     /locus_tag="WGR04_000726"
     CDS             14162..14653
                     /gene="ilvN"
                     /locus_tag="WGR04_000726"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ0794320.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            14720..15013
                     /locus_tag="WGR04_000727"
     CDS             14720..15013
                     /locus_tag="WGR04_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ0794321.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            15093..16106
                     /gene="ilvC"
                     /locus_tag="WGR04_000728"
     CDS             15093..16106
                     /gene="ilvC"
                     /locus_tag="WGR04_000728"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ0794322.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
     gene            16160..16261
                     /locus_tag="WGR04_000729"
                     /pseudo
     CDS             16160..16261
                     /locus_tag="WGR04_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(16330..17325)
                     /locus_tag="WGR04_000730"
     CDS             complement(16330..17325)
                     /locus_tag="WGR04_000730"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ0794323.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGCEVQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGACATYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPASIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(17467..20052)
                     /gene="acnB"
                     /locus_tag="WGR04_000731"
     CDS             complement(17467..20052)
                     /gene="acnB"
                     /locus_tag="WGR04_000731"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ0794324.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKDPIIEYMKSN
                     IVLMKNMIANGYQDPRTLERRIKAMEAWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKSDIPVRLWVAPP
                     TKMDAKELSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVHEGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDQIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            complement(20363..21433)
                     /gene="lptG"
                     /locus_tag="WGR04_000732"
     CDS             complement(20363..21433)
                     /gene="lptG"
                     /locus_tag="WGR04_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ0794325.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGIWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            complement(21430..22545)
                     /gene="lptF"
                     /locus_tag="WGR04_000733"
     CDS             complement(21430..22545)
                     /gene="lptF"
                     /locus_tag="WGR04_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ0794326.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRLV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLDLRHGYRYSGTPG
                     RADYNQVSFQKLSLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLVYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            22715..24121
                     /locus_tag="WGR04_000734"
     CDS             22715..24121
                     /locus_tag="WGR04_000734"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ0794327.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTDNGLQAIAVCRLKSTDRAALNKVAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASRNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEDYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            24184..24624
                     /locus_tag="WGR04_000735"
     CDS             24184..24624
                     /locus_tag="WGR04_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ0794328.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSETSVLLACGGNLPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            24883..25701
                     /locus_tag="WGR04_000736"
     CDS             24883..25701
                     /locus_tag="WGR04_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0794329.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGTVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            25808..26434
                     /locus_tag="WGR04_000737"
     CDS             25808..26434
                     /locus_tag="WGR04_000737"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ0794330.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AERGIPTDRLNHKNFTSRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            26450..27122
                     /locus_tag="WGR04_000738"
                     /pseudo
     CDS             26450..27122
                     /locus_tag="WGR04_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
     gene            27240..27662
                     /locus_tag="WGR04_000739"
     CDS             27240..27662
                     /locus_tag="WGR04_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ0794331.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGKAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
BASE COUNT         6569 a         6895 c         7674 g         6862 t
ORIGIN      
        1 tcgctgggta tggtttgccg ccataagctg tctgctgttg tccgccgttt ttattgcacc
       61 ttacctgacg gcatttcacg aacaagaaaa aacattagaa tatgcggaat tgaccgttac
      121 cgcccccaac cgcagcggac gggcaatcaa actggatgca gacgggcagc attaccggct
      181 ttcctgctat gggttcgaca gtttgtgcac aggcggcaat atcggcagaa ccatcagggc
      241 ggagcaggtt agagccgttt taagcgaaaa cgtcggcaaa ggttttttaa acggcgttct
      301 gttggaatac cgcaacagtg gcagtgtcta taagcaataa agattttgcc cgcacggaag
      361 actgccttgt cgaagtgttg gcacaacctg ccgttttcag cctgaaactg ggtattttgc
      421 ttttattgcc cgccattttc ctgcgtttga aaagaatgtg aaaacaaatt ggcggggcgg
      481 gggaatgccc atgcggcaat tttacgtttt gggttatgag gataaccgtt ataatcacaa
      541 ctttacagtc tgcacggaga aaaccgatgc cttggaatct ccccatcttc ctcacatggt
      601 tgagggtctt gctcattcct gtcatgacag ccctttttta cctgcctttt ccgtggttcg
      661 cggaggaaac gattaatctc accgccgccg tcatttttgc cgttgccgcc ttgaccgact
      721 ggtttgacgg atttttggca aggttgtgga aacagacctc ggatttcggc gccttcctcg
      781 atcccgttgc cgacaagctg atggtcgccg tctctttgct tttactggtc aaactcgacc
      841 ggacctatgt tttgtttgcc atgatcatca tcggcaggga aattaccatt tccgcattgc
      901 gcgaatggat ggcgcaaatg ggcaaaagga acagcgttgc cgtcgccacc gtcggtaagt
      961 ttaaaaccgc cgcgcaaatg ctggcaattt tcctgctgct gctgaatatt cccgattttt
     1021 acggatttaa tcttgttgtt atcggcaaca tattgatgtt tatcgcatct ttgctgacag
     1081 tctggtcgat gctgtattat ctgaaaatgg cgtggaaaga aatcgcctga aaaaaacata
     1141 aaaatagctt gacggcaaaa acagaatcca taataattgc gtctcttcga tgttggagaa
     1201 tgaaatcggg cgggaatagc tcagttggta gagcgcaacc ttgccaaggt tgaggtcgcg
     1261 agttcgagac tcgtttcccg ctccaaaaat ttcattttcc cacaatcatt gcgggaatag
     1321 ctcagttggt agagcgcaac cttgccaagg ttgaggtcgc gagttcgaga ctcgtttccc
     1381 gctccaagtt ttatttcaaa cgtatcaacg cgggaatagc tcagttggta gagcgcaacc
     1441 ttgccaaggt tgaggtcgcg agttcgagac tcgtttcccg ctccaaaaac cgatatgttt
     1501 gaacgcgcgg gaatagctca gttggtagag cgcaaccttg ccaaggttga ggtcgcgagt
     1561 tcgagactcg tttcccgctc caagttttta gattaagcca gttttaggcg agatagcaaa
     1621 gtggttatgc agcggattgc aaatccgtct acgccggttc gattccgact ctcgcctcca
     1681 ttatcgtact atggcggggt ggcagagtgt ttatgctatg aggagtgcaa tcttcatata
     1741 ggccggttaa aatccgcgcc cccgcctcca cctttcacaa atgcccgggt ggtgaaatag
     1801 gtagacacaa cggacttaaa atccgtcggg actaaacatc ccgtgccggt tcgattccgg
     1861 ctccgggcac caagctgaaa atgacaatac cgctccaagc ggttattttt ttatctgtcg
     1921 gacggtgatg gacaatattg acatgttcat gcctgaacaa gaggaaatcc aatcaatgtg
     1981 gaaagaaatt ttactgaatt acggtatttt cctgctcgaa ctgcttaccg tgttcggcgc
     2041 aattgcgctg attgtgttgg ctatcgtaca gagtaagaaa cagtcggaaa gcggcagtgt
     2101 cgtactgacg gatttttcgg aaaattataa aaaacagcgg caatcgtttg aagcattctt
     2161 tttaagcggg gaagaggcaa aacatcagga aaaagaggaa aagaaaaagg aaaaggcgga
     2221 agccaaagca gagaaaaagc gtttgaagga gggtggggag aaatctgccg aaacgcaaaa
     2281 atcccgcctt tttgtgttgg attttgacgg cgatttgtat gcacacgccg tagaatcctt
     2341 gcgtcatgag attacggcgg tgcttttgat tgccaagcct gaagatgagg ttctgcttag
     2401 attggaaagt ccgggcggcg tggttcacgg ttacggtttg gcggcttcac agcttaggcg
     2461 tttgcgcgaa cgcaatattc cgctgaccgt cgccgtcgat aaggtggcag cgagcggtgg
     2521 ttatatgatg gcgtgtgtgg cggataaaat tgtttccgct ccgtttgcga ttgtcggttc
     2581 ggtgggtgtg gtggcggaag taccgaatat ccaccgcctg ttgaaaaaac atgatattga
     2641 tgtggatgtg atgacggcgg gcgaatttaa gcgcacggtt acttttatgg gtgaaaatac
     2701 ggaaaagggc aaacagaaat tccgacagga actggaggaa acgcatcagt tgttcaagca
     2761 gtttgtcagc gagaaccgcc ctcaattgga tattgaggaa gtggcaacgg gcgagcattg
     2821 gttcggtcgg caggcgttgg cgttgaactt gattgacgag atttcgacca gtgatgattt
     2881 gttgttgaaa gcgtttgaaa acaaacaggt tatcgaagtg aaatatcagg agaagcaaag
     2941 cctgatccag cgcattggtt tgcaggcgga agcttctgtt gaaaagttgt ttgccaaact
     3001 tgtcaaccgg cgggcggatg tgatgtagtt tgccgaacgg atggaaatgc cgtctgaagc
     3061 gtgttcagac ggcatttttc aagttccggc tttgatgttc cgttccgacc actctatccg
     3121 ttgtccgatg tctttcaaat tggaatattg ttcgaccaat cgcggcacgt cgtccaaact
     3181 catggcaaac atccacagat aagtgccgac cgaatagaca tgccccgcgc tgctgtagcc
     3241 tttgagcgtc atcatcacaa aagcaaagcc gaacaaaata cccatcgccg tgccgacgca
     3301 gagatagccg aaggcttcgc ggttggaaat cagcacacgc aggcgcgcaa gcagtccgta
     3361 atggcggtac agctgccgcc ggtcgccttt tcggataaag tggttgtcgc gttccaagct
     3421 gttcttcagg cggaaataca ggttttcgct gatggcggca aaacgtggca aaagccataa
     3481 aaacaacgca agtatgccca ccgccgacac gccgacccaa aattccagca ccagcagcat
     3541 gatgcacgcg ccgaatatgg atacgacgga tgtcgcggca atcggcaggt gttcttcaaa
     3601 aaagctgaca aactcacgcg acagggcaac ccgtgcagtt atagtggatt aacaaaaatc
     3661 aggacaaggc gacgaagccg cagacagtac aaatagtacg gcaaggcgag acaacgccgt
     3721 actggtttaa atttaatcca ctatattgcc gaatgcggga cttaccgctg ccgctgttcc
     3781 aacacaaccg gcacggcgat ttcggtataa atccgcgtaa acgtgcgtgt atcggcaatc
     3841 cgccgcgccg caccgaccag ccacattaaa agcacaacca aagcgtacag caacgcctgc
     3901 cacaccttcc ccgcaattac ggcattaatt gcccagccgc caaacaccgg atacaccagc
     3961 atcaaaaggt tttccagtcc gaccagaaaa aatgtgccaa tcaatcgctt gcggtggttt
     4021 tggctatgtg tttcaacatt ttccacatct ttgctcccta ttttttattt tccgagattg
     4081 ttttttccat cacttccgcc agtgcatcca aatcggcaaa cagccgagtt gtgcgcttgt
     4141 cgccgaatgt ggcaaatact ttgtcgctga attcctgcgc gctttctgtt aaaggtgcgg
     4201 cataggcttt gcctgtttcg gtcaacgaca gcaaccgttt gcgccggtcc tgttcgcctt
     4261 cctgccattc aatcaaccct tgtccggcaa gggttttgca tacgcctgaa acggtctgtt
     4321 tgggcaggct ccacttttcg ccgatatgct tttgcgtgcg gctgccttcg gttgccaggg
     4381 tataaagtac ggcaaagagg ttgtaattca gatcctgctg cccgatccat ttgtcgaaga
     4441 cattgcaaat caggttgata cgggtgccaa gttggtcgag tcggttcatt attggtcttg
     4501 atattgacta aatttgtgca ggttatagtc ataatcggga ctgattttaa gcgtggggtg
     4561 ataaaaatgc cgtctgaaat atgttcagac ggcattgtgt ttggaaaagg caatcagtgt
     4621 tctgccagtt tcaggtaaac gccggaaacg tcctgtttgc cgtagccggc ttcgactgct
     4681 ttgcggtagc tggcagcaac ggtttcgacg gcgggcaggg tgttgcctgc ctgttcaagc
     4741 tctttgacgg cgaggttgag gtctttggag gcgtgtttga gggcgaaggc gggtgggaat
     4801 tcgcggtttg cccacaggga ttttttggtt tggaacatgg gcgagtccat tgccgagccg
     4861 ccgatggctt cgacgatggt gtcggtatcg atgccgaact gccgcgccat cagcatcgct
     4921 tcgctgtacg cttcgccgaa aatgcccaag agcgagttca agacgagttt cgcgcccgaa
     4981 cctttgccga catcgccgaa atggaaggtt tttttgccga cgagggaaaa tattttttgc
     5041 agcgggttta aaacggcttc gctgccgccg aacagaatca gcagcgtgcc gttggtggcg
     5101 ggcccgaccg atccggaaac gggtgcttcg gcaaactgtc cgcctgcggc ttcgacaagt
     5161 gctttgacgg cgaggttttc ggtcggggag atggtgctca tgttgacgat gattttgccg
     5221 gccaatccgt cgcggactcc gttcaggatg tcgcacacgg cggcatagtc ggaaaccatc
     5281 aggaaaatga cgggatagtc gcggacgagt tcggcggtgt tgccgtaaac ttttgcgcct
     5341 ttggcggaga tgggggcagt tttgtcgggc gagcggttgt atacgccgac ttcgatgccg
     5401 ccgtccaaga gccgcgttac cataggcaga cccatttgcc ctaagcctat ccagccgatt
     5461 tgtgcgtatt cgtttgcgga catgatgttt actccttttc tgtttgtagt gtaatcttat
     5521 ctgttccaat accgtctgaa aagcctgcgg gtgtttcaga cggtatggtt ttttatgcgg
     5581 tcagcgtgcc tttggtcgag ggggtctgtc ctgccatgcg cgggtcgtgt tcgactgcca
     5641 tacgcagggc gcgcccgaag gctttgaata cggtttccgc ctgatggtgg gcgtttttgc
     5701 cgctgaggtt gtcgatgtgc agggtcatca tactgtggtt gacgatgccg tggaaaaatt
     5761 cttcaaacaa atcgacatcg aaacgtccga ttagtgcgcg ggtaaattcg atgttgtaca
     5821 cgagtccggg gcggccggaa aggtcgatga cgacgcggct gagggcttcg tcgagcggga
     5881 cgtaggaatg tccgtaacgg cggatgcctt ttttgtcgcc gagtgcctgc cggattgctt
     5941 gtccgagtgt gatgccgatg tcttcggcgg tgtggtggtc gtcgatgtgc aggtcgcctt
     6001 tgcagctgat gtcgatgtca atcatgccgt ggcgggcgat ttggtcgatc atgtgttcga
     6061 ggaagggtac gccggtatcc agcctgcttt tgccgctgcc gtcgaggttg atggagacgg
     6121 tgatttgggt ttcgcaggta ttgcggttga cggtaacgct gcggcagccg gcagcagatg
     6181 cggtttcggc aatttgggtt tcggcagttc cggtgctttc tgcggcggtt tcggggacag
     6241 tgcgttcgcg gtgttttctg tcgagccagc ctttgggttt gccggtgtgt ttttcgagtt
     6301 ttgccatcag gctgggacgg atgccgcgtg catctgggtc ttctgcctgc ttttcaaggc
     6361 gttgtttgag tttgtagagt gcgacggggg tacggtagcc gcagagtttg gcgagcttgg
     6421 gcagtgaacc tgcttcttgg gcgagggtca ggaagttgtg cagttggcgt tgtgttttag
     6481 tcaaattcat aggaaatccg ttggttggca gtaaagttgg cgaatgatgt tgagaacggc
     6541 atcgttttgt gcggggctgc ctacggtaat gcgcaggcag tgttccaaaa gcgggtgcgc
     6601 gccatgcagt tttttaacca agatgcggtt ttgtttgagc gtgtcaaaca acaaatcggc
     6661 atcgggtacg cgtatggtaa tgaagtttgc ctgacttgga aaggtgttca gacggcatat
     6721 tttgcccaat tcggcgaaca tccgttcgcg ttcgtttttc aggctgtcga tgttggcaga
     6781 gatgatgccg tagtgttgca gggcgagttt ggcggtggtc aggctcagtt ggttcatatt
     6841 gtagggcggc aggatttttt gcagttcgcc gatgacttcg ggacagcctg ccgcatagcc
     6901 gatacgcagt ccggcaaaac cgattttgct gacggtacgc atgacgatga ggttgggaat
     6961 gctgcccgcc tgcggcagga agctgtcgcc gttgaatgcg ccgtaggctt cgtcgacgac
     7021 gacgatgccg tctgaagctt cgatgacggc ttcgatttcg gcacgcgtga aacatacgcc
     7081 ggtggggttg ttggggtagg cgataaaggt cagggcaggg cggtgcttcc tgacggcttc
     7141 gaggacggcg ggcaggttga gggtgaaatc tccgttcagt ggaacgccga cataatccat
     7201 gccgtacagc gcggcgttgt ggcggtacat gacgaaactg ggttcggctg ccaacattgc
     7261 cgcgcccggt ttggcggtca gcatcgtgat gaactgtatc agctcgtccg agccgttgcc
     7321 cagcgcgatg tcggcgcagt cgggaatgtc gaacgccgaa cgtaatgctt cctgtaaacc
     7381 gctgccggag ggattggggt aaagatggat gggcgcggcg gcaagccgtg cctgccattc
     7441 ctgcaacagc gtttcatgtc cggcaaaagg gtggacggga ctttccatcg aatcgagttt
     7501 ggcaaagccg ggcggaacgt cggcaatctg atatgccgac atagcttgta tgtcgtcgcg
     7561 gatgaaggag cggacggatt tcatcgtgtt tccttaatgg ttggaatatg cctgtacgcc
     7621 gtttcggcat tatttcatac ggaactctgc cgcgcgggcg tgggcggtca ggctttcgcc
     7681 gtgtgccagc acgctggcgg tttcgcctaa tttttgcgcg ccctgttccg aaacctgaat
     7741 caggctggag cgtttttgga aatcatatgt ccccaaaggc gaggaaaagc gggcggttcg
     7801 gctggtgggc aatacatggt ttggaccggc gcagtagtcg ccgaggcttt cgccggtgta
     7861 gcgtcccatg aaaatcgcac cggcgtggcg gatttttttc gcccattcct gcggattttc
     7921 gactgacagt tccaagtgtt cgggggaaat gtagttggcg atttcgcagg cttcgtccaa
     7981 gtctttggcg agtatcatcg cgcccctgtt gccgagcgaa gcttcgatga tgtcgcggcg
     8041 cggcatagtt tcgatcaggc ggttcatggc ggcttctact tcgtcgagat acgcttgcga
     8101 cgtgccgatg aggatggctt gggcaatttc gtcgtgttcg gcttggctga acaaatccat
     8161 cgccacccaa tcggcgggtg tcgtgccgtc ggtgatgacc aggatttcag acggccccgc
     8221 caccatgtcg atgccgacca cgccgaacac gcggcgtttg gcggcggcga cgaaggcgtt
     8281 gcccggaccg gtgattttat cgacttgggg gatggtttcc gtgccgtagg cgagggcggc
     8341 aaccgcctgc gcgccgccga cggtgaagac tttggttacg ccggcgacgt atgcggcggc
     8401 aagcacgatg tcgttgcgtt cgccttttgg tgtcggcacg accatgatga tttctttcac
     8461 acctgcgacg tgggcgggca tggcgttcat gatgacggaa ctcggatacg ccgccttgcc
     8521 gccggggacg taaatgccga cgcggtcaag cggtgtgatt tgttgtccca acagcgtgcc
     8581 gtcttcatcg gtgtagctcc acgattccat tttttggcgt tggtggtagc tttcgacacg
     8641 gcgggcggcg gtctgcaatg ccgtctgaac gtcgttcgga atgcgctcga acgccgcgtt
     8701 caaatcggct tgcgtgagta ttaaatcatc gatgctttta gcgtttgtct gatctaattt
     8761 gttggtgtat tcaatcaaag ccgcatcgcc gcgcttttgc acatcggcgc aaatgtcggc
     8821 gacgatgcgt tcggtttcgg ggttttgtgc ggtctcaaaa gccagcaggg ctttgagtcc
     8881 ggtttggaaa tcgggtgatt gggtgttgag ttttttcatg atttggtttt ttcctttttt
     8941 atttttggat tgggggtcgt ctgaatattc tcttgttcag tttctgtctc tgttttgggt
     9001 ctgatatacg ctttttctcc cccgctacca aacttcaatt cgggcaggca tttttgttgc
     9061 agaccgagtt tggctaatac ctcttcgcta cgagaggtag tgcggaaacg gtaacgggat
     9121 tgttttctac cgtttttggt ttcctcaacc attgtttcaa aaccgtccac ttcgtatgca
     9181 ctgacaacag cgttttgcag accggtgtga acgccgataa cgtatttcac ttttgaagca
     9241 acatctttac cgataaccca gttgcccaac gtacgcgatt tgaggttggc atcgtcttgg
     9301 ttgtcgaaaa ggtagtctaa ttcttcgtca gtatctaaat cgaaagcatt gtggattttg
     9361 atggcgagaa tcagcccgtc ggggttaatc tcgttaagtg ggagagaaga gaatccaaag
     9421 cggcgatcta gttcttctac gctaatacca cccggaccat gcccggcaat tttatttttg
     9481 agttttttac ccaagacaga tttaacaaaa tgaattaagg cagattcggc ggcgagtgct
     9541 tcgacttcag tcagatgata gctgatgata tagcgaccga gtttcttgca tttggagatg
     9601 gctttgagtt tccgatcgat aatctcgccg gagactgggt cttgtgaacg actagcaacc
     9661 cattcatgct cgaagatacg attaccgcag cctttgccga tgtagaggat tttgtctttt
     9721 ttcaggtcgg tcagacaata gacataaaac cgacgctcgc cgttattcaa aacggaaagg
     9781 gttgaatctg agaatttttt tatttttgca accattttaa cttctcctat acagtttact
     9841 gccggtgata ttttcagacg acctgtcggc gggaacggat agggtcgtct gaaaacgcat
     9901 ctttattcaa atggatgctt acttcgcctt cactgcgccg ccgaacgcct gaataatcgg
     9961 ctccagcagc gcgtatttcg ttttcaaagc agccttgttg accaccaggc ggctggaaat
    10021 gtcgacgatg tgttcgactg cttccaagcc gtttgccttc aaggtgttgc ccgtcgaaac
    10081 caagtccaca atcgcatcgc tcaagccgac cagcggcgca agttccatcg agccgtacag
    10141 tttgataatg tccacatgga cacccttgcc ggcaaaatga gatgccgcga tttcaggata
    10201 cttcgtggca atcttcagac ggcatccggg ttgcgaagct gcttcgtaat caaacccttt
    10261 acgcacagca accatcatgc ggcacttggc aatctccaaa tccaaaggcc ggtaaagccc
    10321 cgtgccgccg tgttcgatca gcacgtcttt gcccgcaatg ccgaagtccg ccgcgccgta
    10381 gcggacataa gtcggcacat cggttgcgcg gacaatgaca aggcggatgt tttcatggtt
    10441 cgtcccgata atcagcttgc gcgatttttc aggctcttca gtcggaacaa tgccggcagc
    10501 ggcaagcagc ggcagcgtct cctcaaaaat gcgccccttg gataaggcga tggtcaaagc
    10561 attatcctgc atggtgtgga tgtcctttgt cggttgaaac aataatgccg tctgaatagg
    10621 cgccgacggt gcagacggca tacggtttta tcagagcaga gtccttacat cggcagcaat
    10681 cgtttccggc tcgcttccgt aaggcgagaa aatggcaacc tcaccgtttt tgtcgatgag
    10741 atacgcaccg gaagagtggt cgaccaaata gttttcgctg tcgtcttttt gattgacttt
    10801 ggcagaaacc acgcggtatt gctgcttgat gaccggcagg ttttggccgc ccgttgccgt
    10861 cagaccgata aagtccggat tgaactgttt ggcatacttg ccgatgattt caggcgtgtc
    10921 gcgttccgga tcgatgctga cgaacaccac tttcacgtcc ttagcctgcc cgcccaactg
    10981 cttcaacgtg tcgctgtacg tcaaaagctc tgtcgggcag acatcgggac agtgcgtaaa
    11041 gccgaaagac agaatcacga ccttgccttt caaatcgctc aggttgaaag gcttgccttc
    11101 gccgtcggtc agcgtgaaat cgccgccgat gtcttcctta cgcatatccg taccgcgcgt
    11161 ttgcggcttt gccgcatttt ccgcagccga cgcggatgca cttgaagaag cggcttgcgc
    11221 cgcactgttg tcttgaggtt tgcaggcggc aagcgcggca agtacgaaaa cgcccggcaa
    11281 aaaggaacgc ggtacggaaa acatgacaat ctcccaagaa taaatagcaa aacctgattc
    11341 gtacggaatt tgaatatttc catcttaacg cgtttggcag atttaatgta gtattttttt
    11401 acgttgtctg cacggcagga atagccgaat catacagaat ctttggagcg gaaagaacag
    11461 taagattaaa ataataaaaa ttctatttaa aatcaaaaat aaaataaaaa cagataaaat
    11521 aatttcaaaa aaaaggttta cagaatttca aaagccatta taatgacgac ttcttcacca
    11581 gcccaggtgg cgaaattggt agacgcaggg gactcaaaat cccccgccgc aaggtgtgtc
    11641 ggttcgagtc cgaccctggg caccacaacc gcttttagag cggttatttt tttgtctatt
    11701 gctgtttaat gaaatagata agactaaata tagtggatta aatttaaacc agtacggtgt
    11761 tgcctcgcct tagctcaaag agaacgattc tctaaggtgc tgaagcaaca agtgaatcgg
    11821 ttccgtacta tttgtactgt ctgcagcttc gtcgccttgt cctgattttt gttaatccac
    11881 tatacaatca atatattaag tcgttgttag ccctcttggt tctgtcaatg ccgtccaata
    11941 cagtccggtg caatgagcca ttttcaggaa gtatggttgg gaatattttt gatatatcaa
    12001 aaataaaatc aagttcaatc aatatcccga gtctttattg ggatgaaacc ctgtaaattt
    12061 taaaattctt gatatcgaag aagggttttt cacatctgcc cctcctacac aaactgtcaa
    12121 caatttccat ctttcaaaaa aggtactcct gattttattc ttttaaaatc aatcaataat
    12181 ttctctgctt tcaaaaaata ttaaaagatg aaagattttt cttgattgaa ctgtcggttt
    12241 gcgttatcgt ttccactctt tttcacaaac tctgtgttcc tttccaattg attggataat
    12301 ccgtctgttg ccgtgttccc ccaatgcgcg taccctaaat cgctgcttgg tggaattgca
    12361 ttacaggtgc tgtggcaaag cggtttgtcc ctattggttt gaaaccgtat aaaagaggtc
    12421 gttatgcagt tatcaggcgc gcaaatcata gtgcagagtc tcaaagccga aggtgtcgag
    12481 tacgttttcg gttatcccgg cggtgcggtt atcgaaatct acgatgccct tttccaactc
    12541 aataaattca agcacattct gacccgtcac gagcaggcgg cagtacacgc ggcagatgcg
    12601 tatgcgcgcg tcagcggtaa ggtgggcgtg gcattggtta catccggccc gggcgttacc
    12661 aatgcactga ccggtattgc tactgcctat acggattcga ttccgatggt ggtcatcagc
    12721 gggcaggtag gcaattccct gattggtacg gatgcgttcc aagaagttga tacggtgggt
    12781 attacccgtc cgtgcgtcaa acacaatttc ctggttacgg acatcaatga gttggcggaa
    12841 accattaaaa aggcgttcca aattgccgca agcggccgac cggggcccgt ggtggttgat
    12901 gtcccgaaag atgttacgca ggcgatggcg aaattcagct atcctcagga agacattttt
    12961 atccgttcgt atcaaccggt tgttcaaggg catatcggtc agattaaaaa ggccgtgcag
    13021 atgttggcat ctgccaaacg tccggtcgtc tattttggcg gcggcgtggt gttgggtaat
    13081 gcttctgaag agctgacccg atttgtccga atgacgggtg ctccgtgcac gggtacgctg
    13141 atgggcttgg gcgcttatcc ttccggcgac cgccaattcc tcggtatgct cggtatgcac
    13201 ggtacttacg aggcaaacct tgccatgcag aatgcggatg tcgttcttgc cgtaggtgcg
    13261 cgttttgacg accgtgtggt ttccgtaccg tccaaatttt tcgagaaggc gaagaaggtt
    13321 atccatatcg atgtcgatcc ttccagcatc gccaaacgcg tgaaggtgga cattccgatt
    13381 gtcggcgacg tgaaaaatgt tttgtctgag atggttgcgc tgtggcaaaa acaagagttc
    13441 atgccgtctg aagatgcttt gggcaaatgg tggaaaacca tagaggaatg gcgttcccaa
    13501 gattgcttgt ggtttgacaa cggcagcgaa attatcaagc cacaatatgt gattcagaag
    13561 cttgccgaga ttaccggcaa ttcggcaatc atcacatcgg atgtagggca gcatcaaatg
    13621 tttgcggctc aatattatcc cttcgaacgt ccgcgccaat ggctcaattc cggcggtttg
    13681 ggtacgatgg gcgtgggtct gccttatgcg attggtgcaa aacttgccgc cccggatcaa
    13741 gacgtattct gtattaccgg cgacggctcg attcagatga acatccaaga gttgtccacc
    13801 tgtttccaat atcggattcc ggttaacgtc attacgctga acaacggtta tctcggtatg
    13861 gtacgccagt ggcaggaaat atattacggc ggccgagagt cggaaactta tttcgattct
    13921 ttgcccgatt tcgtcaaact tgccgaggca tacggccata tcggtatccg cgtggacaag
    13981 aagtctgatg tggaaggtgc gttgttggaa gcattgaacc aaaaagacag gctggtgttt
    14041 atcgacttcc tgaccgacca gaaacagaat gtgatgccca tggtcggcaa cggcaaaggt
    14101 ttggacgaaa tggtacttcc gccgcatatg cgtgcggacg aaaaggcgta aggagagaca
    14161 aatgcgacat atcttatctg ttctgattga aaacgaatca ggtgcgatga gccgcgtggt
    14221 cggtttgttc tctgcacgcg attacaatat cgattctttg gcggttgcgc cgaccgaaga
    14281 caaaaccctg tcccgtatga ccatcgttac ccacggcgac gagcaggtta tcgaacaaat
    14341 caccaagcaa ctcaataaat tgattgaagt gattaaagtg gtcgatttga acgaaagccg
    14401 ttttgtcgaa cgagaactga tgttggtaaa agtccgtgcc gccggtaaag accgcgacga
    14461 atttttgcgt ttgaccgaaa tctaccgggg cagcatcatc gacgtaaccg accgcagcta
    14521 tacgattgaa attaccggct cgaccgacaa gctggattcc tttttggaaa cggtcggacg
    14581 cgcccaaatt ttggaaaccg tacgtacagg cgcggccggc atcggtcgcg gtgagcgtat
    14641 tttgaaaatt taacgccgta acctttcaga cggcacggta tttgaatgcc gtctgaaaaa
    14701 cgaacggcag gagagattta tgtcgaacat taaaattgtc gcactggtta ccgtcaaacc
    14761 ggaatacaca gaaacactgg cagcacagtt taaagaactg gtcaaagcca gccgtgcaga
    14821 agagggcaac atcagctacg atctccatca ggaaatcggc aaaccgaacc gttttgtttt
    14881 cgtggaaaat tggaaatctc aagcagctat tgacgaacat aacgccagcg cgcatttcca
    14941 agccttcgtc cagtccgtcg acggcaaaac cgaagcgttg gaaatcgtat tgatgaatga
    15001 agttgccgat taagcggcac actctgttta acccgtccga agccgctcaa cattttaagg
    15061 cttcgacaac catttacctt aaaggaaatc aaatgcaagt ctattacgat aaagatgccg
    15121 atctgtccct aatcaaaggc aaaaccgttg ccatcatcgg ttacggttcg caaggtcatg
    15181 cccatgccgc caacctgaaa gattcgggtg taaacgtggt gattggtctg cgccaaggtt
    15241 cttcttggaa aaaagccgaa gcagccggtc atgtcgtcaa aaccgttgct gaagcgacca
    15301 aagaagccga tgtcgttatg ctgctgctgc ctgacgaaac catgcctgcc gtctatcacg
    15361 ccgaagttac agccaatttg aaagaaggcg caacgctggc atttgcacac ggcttcaacg
    15421 tgcactacaa ccaaatcgtt ccgcgtgccg acttggacgt gattatggtt gcccccaaag
    15481 gtccgggtca taccgtacgc agtgaataca aacgcggcgg cggcgtgcct tctctgattg
    15541 ccgtttacca agacaattcc ggcaaagcca aagacatcgc cctgtcttat gcggctgcca
    15601 acggcggcac caaaggcggt gtgattgaaa ccactttccg cgaagaaacc gaaaccgatc
    15661 tgtttggcga acaagccgta ttgtgcggcg gcgtggtcga gttgattaaa gcaggttttg
    15721 aaaccctgac cgaagccggt tacgcgcctg aaatggctta cttcgaatgt ctgcacgaaa
    15781 tgaaactgat tgttgacctg attttcgaag gcggtattgc caatatgaac tactccattt
    15841 ccaacaatgc ggagtacggc gaatacgtta ccggccctga agtggtcaat gcttccagca
    15901 aagaagccat gcgcaatgcc ctgaaacgca tccaaaccgg cgaatacgca aaaatgttta
    15961 tccaagaggg taatgtcaac tatgcgtcta tgactgcccg ccgccgtcta aatgccgacc
    16021 accaagttga aaaagtcggc gcacaactgc gtgccatgat gccttggatt actgccaaca
    16081 aattggttga ccaagacaaa aactgattgt tttcaaacgg gactgcctac acatggtgta
    16141 ggcagttttt ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt
    16201 acaaatagta cggcaaggcg aggcaacgcc gtactggttt aaatttaatc cactatatat
    16261 ggataccgtc tgaacatcgt gttcagacgg tatctgtgtt gcggatgaat ttaaacaggc
    16321 acagttctgt cagtcgccca aagccgcgac cattaccgct ttaatcgtgt gcatacggtt
    16381 ttccgcctga tcgaacacga tgctggcggg gctttcgaat acttcttctg taacttccac
    16441 accgttcagc ccgaaggttt cgtaaatcca ttcgccgact ttggtttcgc ggttgtggaa
    16501 ggcgggcagg cagtgcatga atttgacttg cggattgccc gatgccgcca tcagttcggg
    16561 cgtaacgcgg taatctttca gcaaatcgat gcgttcctgc cagacttctt tcggctcgcc
    16621 catgctgacc cacacatcgg tatgaataaa atcaacattc ttcacggctt catgcgcgtt
    16681 ttcggtcagg gtaatttttg cgccggtttc tttggcggcg gcgtgtgcgg cggcaataat
    16741 gccttcagac ggccacaggc tttgcggtgc gccgatacgc acgtccatcc ccaattttgc
    16801 ccctaaaatc agcagggaat tgcccatgtt gtaacgcgcg tcgccgacgt aggcaaacgc
    16861 ggtttggttc aaaggtttgc cgctgtgttc gcgcatagtc agtgcgtcgg caagcatttg
    16921 tgtgggatgg aactcgttgg tcagcccgtt gaacacgggt acgcccgcgt atttcgccaa
    16981 ttcttcaaca acttcctgac cgaagccgcg atattcgatg gcatcgtaca tcctgcccaa
    17041 gacgcgcgcc gtgtctttga tgctttcttt gtgcccgatt tggctggcag acggttccag
    17101 ataggttgca cacgcgcctt gatcgcgtgc ggcgacttca aatgcgcagc gtgtgcgtgt
    17161 ggatgttttt tcaaaaatca gggcgatgtt tttccctttc atccgctgca cttcgcaccc
    17221 tgcctttttg gcggctttca attcggcggc aaggtcgagg taggcggtga tttcttccgg
    17281 cgtgaagtct aaaagtttca gaaaatggcg gtttttcagg ttcactgtcg tttcctttat
    17341 gtgggggcgt gaacgccgta tttgcagcgt ttacggaaag aagggttagc ggaatgttgc
    17401 aatgccgtct gaaggttgtt cagacggcat tgcggtcaat cccttactcc atacggatga
    17461 cgggaatcaa acattcacgg tttcggctac ttcgttgtag ctgtcgattt cgttgaagtt
    17521 catgtaacgg tagatttggt cgccttgctc gttgatgatg ccgatgttgg cttggtattc
    17581 ttcaacggtc gggattttac ccagtttgga gcaaatcgcc gccaactcag ccgagccgag
    17641 gtaaacgaag gtgtttttac ccaagcggtt cgggaagttg cgggtcgaag tggacatgac
    17701 ggtcgcgcct tcgtgcactt gggcttggtt gcccatacac aatgaacaac ccggcatttc
    17761 catacgcgcg ccggcgcggc cgagtacgcc gtagtgacct tcatcggaca actctttcgc
    17821 gtccattttg gtcggcggtg ctacccacaa gcggacgggg atgtcgctct tgccttccaa
    17881 gagtttggaa gcagcgcgga agtggccgat gttggtcatg cacgaaccga tgaacacttc
    17941 gtcgattttg gtgccggaac gttcggacat gaaacatacg tcgtcggggt cgttcggaca
    18001 agcaataatc ggctctttaa tgtcgtccat gttgatttca atcacggcgg cgtattcggc
    18061 atctttatcc gcttcgagca attcaggatt tgccaaccat gcttccatgg ctttgatacg
    18121 gcgttccaaa gtgcgcggat cttgatagcc gttggcaatc atgtttttca tcaacacaat
    18181 gttggatttc atgtactcga taatcggatc tttgttgagc tttacagtac aacctgcagc
    18241 ggagcgttcg gcagacgcat cggtcaattc aaaggcttgt tccactttca aatcaggcag
    18301 gccttcgatt tcgagaatgc ggccggagaa gatgtttttc ttaccggctt tggcaacggt
    18361 cagcaaacct tgtttaatcg cgtaaagcgg gatggcgttc accaaatcgc gcagggttac
    18421 gcccggttgc agtttaccgc tgaagcgtac caatacggac tcgggcatat cgagcggcat
    18481 tacgcccgtt gcggcggcaa aagcgaccaa gcctgaacct gcggggaagg aaataccgat
    18541 ggggaaacgg gtgtggctgt cgccgccggt gccgacggta tcgggcagca gcaggcggtt
    18601 gagccacgag tggatgacac cgtcgcccgg acgcagggac acgccgccac gggtggaaat
    18661 aaaggcgggc agttctttat gggtttttac atcgacaggt ttcggatagg cggcggtgtg
    18721 gcagaaagac tgcatcacca tatcggctga gaagcccaag caagccaagt ctttcaactc
    18781 gtcgcgggtc atcgggccgg tcgtgtcttg cgagccgacc gtcgtcatac gcggttcgca
    18841 ataagtaccc gggcgcacgc cttgcccctc gggcagaccg caggcgcgac cgaccatttt
    18901 ttgcgccaag gtgaaaccgg ctttgctttc ggcaggcgcc tgcggcaggc ggaatgcagt
    18961 agaggcaggc agtttcagag cttcgcgtgc tttggcggtc agacctcggc cgataatcag
    19021 gttgatacgg ccgccggctt gcacttcgtc cagcaatact tgtgatttca attcaaactc
    19081 ggcaacagtc tcgccgtttt tcacgatttt gccttcataa ggcaggatat cgacgacatc
    19141 gcccattttt agagcagata catcgacttc aatcggcagc gcgccggaat cttcttgggt
    19201 attgaagaaa atcggcgcga ttttgccgcc caaacatacg ccgccgaagc gtttgttcgg
    19261 cacgaacgga atgtcttcgc cggtatgcca aatgacggag ttggtcgcgg atttgcgtga
    19321 agaaccggta ccgaccacgt cgccgacgta agcaaccgga tggcctttgg ctttgagttc
    19381 ttccaacaat ttaatcggac cgacttcgcc cggtttgtcg ggcgtgatgc cgtcgcgcgg
    19441 gtttttcagc atggccagcg cgtgcagcgg aatatcggga cgactccacg cgtcgggcgc
    19501 gggggagagg tcgtctgtat tggtttcgcc gtcaactttg aaaacggtaa cggtaatttt
    19561 ttcgggaact ttggcgcgtg aggcgaacca ttcggcatct gcccaagatt gcaaaacttc
    19621 ttgcgcgtat ttgttgcctt tttcggcttt ttcctgaacg tcgtggaagg aatcgaacat
    19681 cagaagcgta tgtttcaagc ctttggcggc aatgggcgcg agtttgtcgt cgtccaagag
    19741 ttcgattaag gcgtggatat tgtaaccgcc gagcatcgta cctaagagtt cggtcgcata
    19801 ttcgggggag atcagcgggc tggacgcgct gccttcggca acggcagcca ggaatgaggc
    19861 tttgactttg gcggcatcgt ccacaccggg cggaacacgg tgggcaagca gttcgaccaa
    19921 gaactcgcct tcgcctgcgg gcgggctttt cagcagctca accaaatcgg cggtttgctg
    19981 cgcgttcaaa gggagggcgg gaatgccgag ggcggcgcgc tcggcggcgg ctttacgata
    20041 ggcttctaac atctctttgt tcctttttct gtttttcttt tgtcgggttg caaatgattt
    20101 gcgttaaata ttgtaaacaa tatttgtacc gctatcatag actagtttta acaaaaatgg
    20161 aactgttatg acaggtaatg tatagtggat taacaaaaat caggacaagg cgacgaagcc
    20221 gcagacagta caaatagtac ggcaaggcga ggcaacgccg tactggtttt tgttaatcca
    20281 ctataatagg gaacggctat ctgtctacag gaatatgata ataaaaacca atgccgtccg
    20341 aagggggtca gatggcattg gtttagcgtt tttcctgttt gcgtatcagc caaacggcga
    20401 gcaaggcgaa ggctatggta ggtagtgcgc cggcgaggaa gggcgggatg ccgtagagtt
    20461 ggctggtaaa cccgaagagc cgtccggcaa ggtggaacag caatccgaga cagatgccgc
    20521 cgaagagttt taagcccata ttgccgtggc gggtggtttg cggggtaaag gcaaaggcga
    20581 cgagcgccat cacccaggct gcggcggggt aaaccaattt gcgccaccat gcgatggcgt
    20641 agattcgggt gttttggctg ttgttttgga ggtggcggat gtaggtggtc agttcgccga
    20701 cggacatttg gtcgggtttg acgagcaata cgtccatcag gttgcgtttg acggaaatcg
    20761 gccagttttc ttcagccgca atagagacct cgactttgtc ttcgccaagc gtgctgcggc
    20821 ggatgttttt caactgccaa ctgccgtcgc tgttcaaaac ggcggaatcg gcttccactg
    20881 cctctgccag ttcgttttta tcgttgcggg cccagatttt aatgcccagc agggtatggt
    20941 cgggcaacat ttcgcgcaca ttgataatgc tgtttttttc tttgagccaa atgccggtat
    21001 tgccggtgct gattttgccg ttaatggcgg cggctttgat gttttcggct ttttggctca
    21061 gtgtgggcgc aacccattcg ccgagcgcga cggtggcaat agcaaaaata aaaccgaact
    21121 gcgacagaat caacagcagc tttttggtgc tcatgccgct ggctttgatg acggtcagtt
    21181 cgctgccggc ggcaagctgg ctgagagaga ccagtccgcc gataaggacg gcgaggggca
    21241 tcagttcgta ggcgcgggcg ggcattttga gggcggtgta gcccagcatt tcccatatgc
    21301 cgtaactgcc tttgccgagg ttgccggttt cgtacaggat ttcaaaaaag ctgtacaaag
    21361 cgaggaaggc aaggagcgcg taaaccgcca taaccgccat ttgacggatg atgtaacgtg
    21421 aaatcaggtt cattttccgc ctttcaatgt cagacttttg ccaaccgcct gccagaaggg
    21481 ctggctaggc atgctgcgga cgcgcagaag tacgattgcg atgacgaaca tgatgatgtg
    21541 cataggcagc agtccgagcc aaaaatggat tttgccgtct tccacggctt caaaaagcag
    21601 ggtcagcccg ttttggtaaa ccaaaaacaa accgatggca atcaggatgt tgtaggtgtg
    21661 gccgctgcgc ggattgaaat aggaaagcgg cacggcaagc agacacaaca acaaaacgct
    21721 gacggtcaag gaaatacgcc acatcaattc cgcctggtgt tggggattgc tgctgccaat
    21781 cagttgggcg gttggaatgg tgcggcggtg ggaaacaggg tcgatgagtt tgggcgtggt
    21841 gctgataatc aggctgagtt tttggaagga aacctgattg tagtcggcgc gtccgggcgt
    21901 gccgctgtaa cggtagccgt ggcgcaggtc gagcgtgcgt ttgttgtcgt tcagcgagaa
    21961 gttaccttct ttggcgaaga tgatgttgtc gccgccgttt ttgtcctgtt cgcgcaggaa
    22021 caggtttttc atgatgccgg attcggtatc gaaggtttcg acaaaataaa ccctgccgtt
    22081 gcgcttgccc aaactgttga actcgcctgc ctccaccaaa gacaattcct gcttctgctt
    22141 caggatttca gcgtattcgc ggctgcgtag ctctgcccac ggtatcaccc aaagctgcat
    22201 gacggcaacc aaaacggcaa acggcacggc aaactgcatc accaggcgta tccattgttt
    22261 caatgccaat ccgcaggata gccagaccga catttcgctg tcgcgccagt agcgggtcaa
    22321 cacggtcaac gtactgataa atgcggtcaa caccagcaaa agcggcgtca taccgatgac
    22381 ccagaagccg accaatgcca acacggcatc gatggcgaca cgcccgtcgg cggcacggcc
    22441 gagcaggttg attgcctgcg tggagaccaa taccgccaag aggacgacga aaatgccgac
    22501 ggcggtaaaa gagagttctt tgatgaggtt tctttgataa atcatagaat cgcggtcagg
    22561 gggcggtaag cggttcggcg caggcggatt gtccgcgcaa ggtttcagac ggtgcttcaa
    22621 atggggtaca atcgtgccag ccgtcttcag acggtctttt gagcgaaagg ccgtccgaaa
    22681 actaccgaca accgtctcaa tcaggagaat aaacgtggaa tttagcacaa aaaccgaaat
    22741 cttgcaggaa cagcaggcag gcgcacagtt atttgtctgc accgaagagg cgcaattaaa
    22801 ccatccgacc gcccttgccc ttttgtcttc gcttgaagaa ggtcaaaatt ttgccgacac
    22861 aaaaatcccg actgacaacg gtttgcaagc cattgccgta tgccgtctga aaagcaccga
    22921 ccgcgccgcg ctgaacaaag tcgcagccga agccgccaaa tgggcgcaaa atcaggaaac
    22981 ggtcaatgtg gacatccatg ccttcgatga agcgcaagcc gccgccgtag ccgaagcgtt
    23041 tgcgattgcg ttcggcaatg ccgcctaccg tttcgaccgc tacaaaaaag aagccaagcc
    23101 cgccaaattt gaaaccgccg tgttccacag cgcacacgaa gccgccgtta aagaagcctt
    23161 gcgcgtcgcc gaagcacagg tttacggaca aagcctctgc cgcgacttgg gcaatgctgc
    23221 gccgaacgaa tgcacgcccg aatttttagc gcgcaccgcc aaagccgaag ccgaaaaact
    23281 gggcgcgcac gccaaaatca tcgaaaaaga ctacatcaaa gaaaacatgg gttcgttttg
    23341 gtcggtcgcc aaaggcagtg tcgaagatcc ctatttggtc gaactgagct acttcggtgc
    23401 agccgacaaa gaagccgcgc ctgtggtatt ggtcggcaag ggcattacct tcgacaccgg
    23461 cggcatctcc ctcaaacccg gcctgaacat ggacgaaatg aagtttgata tgtgcggcgc
    23521 ggcaaccgtc atcagcacct tctgcgccgc cgtcaaactg caactgccga tcaacctgat
    23581 tgccgtcgtc gccacttgtg aaaatatgcc ttccggcgcg gccaacaaac cgggcgacgt
    23641 cgtaaaaagc atgaaagggc tgaccatcga agtgttgaac accgatgccg aaggccgtct
    23701 gattttgtgc gacgcactca cttacgccga acagttcaaa cccaaagccg tcatcgacgt
    23761 cgccaccctg accggtgcat gcatcgtcgc cttggggcac gatgtcagcg gcgtgatggg
    23821 caacaatcag gatttgatcg acagcctgct cgccgcctcc cgcaacgtgg acgacaaagc
    23881 gtggcaactg ccgctctttg aaacctacaa agaccagctc aagtccaatt ttgccgacat
    23941 tccaaacatc ggcacgcccg gcgcgggcac gattaccgcc gcaacattcc tatcctactt
    24001 taccgaagac tatccatggg cacaccttga catcgcgggt acggcgtgga aatctggtgc
    24061 ggaaaaaggt gcgaccggcc gtcccgttcc cttgctgctg aactatctgc ggaatcttta
    24121 attttgccac aatgccgtct gaagcctttc agacggcatt atcctgccaa atgttcaaac
    24181 aatatgccga aagccacctt ttacacgcac gttgcccaag ttccgatttt cacctgccgc
    24241 ctgattgccc gtgcgattcg ggacggcggc aggatactgg tgtggtccga ttcgttcggg
    24301 cagcttcagg aattggacaa aatgctttgg caatacgagg ccgagagttt cattccgcac
    24361 gaaatttggg aaacggaaga agccatgccg tctgaaacat ccgtcctgct tgcctgcggc
    24421 ggcaatctgc cccgaattcc cgaaggcatg accgttttga acctgtccga cggtttttgg
    24481 aacaccgctc cggtcttgcc cgcgcgcgtt ttggaaatcg tcggcaacag cttggaagat
    24541 ctcgccgacg cgcgcgaacg ctttaccgcc taccgccgaa gcggttttgc catcgaacat
    24601 cacggcatgg agggcaaggc ataaaatgcc gtctgaaatg ccgcatttac aaaatttaaa
    24661 caggcttgat tctatttgat taaaatttcc ttatcggttg aaccccgcca cttggacatc
    24721 tgtccctcgg ggcggtagaa tcaaaactta ttttggaagg ttcaatccct tccaaaacag
    24781 ggcaacacac gattgacgct ttatgtgtca tcctgtgtgt tgaaacattc aaactcggct
    24841 ataatcccgt ttttccgacc ttatcgacag cgaagatcca tcatgaacac cattttcaaa
    24901 atcagcgcac tgaccctttc cgccgctttg gcactttccg cctgcggcaa aaaagaagcc
    24961 gcccccgcat ctgcatccga acctgccgcc gcttcttccg cgcagggcga cacctcttcg
    25021 atcggcagca cgatgcagca ggcaagctat gcgatgggcg tggacatcgg acgctccctg
    25081 aagcaaatga aggaacaggg cgcggaaatc gatttgaaag tctttaccga agccatgcag
    25141 gcagtgtatg acggcaaaga aatcaaaatg accgaagagc aggctcagga agtcatgatg
    25201 aaattccttc aggaacaaca ggctaaagcc gtagaaaaac acaaggcgga cgcgaaggcc
    25261 aataaagaaa aaggcgaagc ctttctgaaa gaaaatgccg ccaaagacgg cgtgaagacc
    25321 actgcttccg gcctgcaata caaaatcacc aaacagggtg aaggcaaaca gccgaccaaa
    25381 gacgacatcg ttaccgtgga atacgaaggc cgcctgattg acggtacggt attcgacagc
    25441 agcaaagcca acggcggcac ggtcaccttc cctttgagcc aagtgattcc gggttggacc
    25501 gaaggcgtac aacttctgaa agaaggcggc gaagccacgt tctacatccc gtccaacctt
    25561 gcctaccgcg aacagggtgc gggcgacaaa atcggcccga acgccacttt ggtatttgat
    25621 gtgaaactgg tcaaaatcgg cgcacccgaa aacgcgcccg ccaagcagcc ggctcaagtc
    25681 gacatcaaaa aagtaaatta agtccgaatc catgcccgaa acaggttttc gggcattttt
    25741 acggcaaatg ccgtctgaaa ccaccaaaca gcggttcaga cggcatccct ttcaaaagcg
    25801 caccattatg aaaaacatcg tcatcctgat ttccgggcgc ggcagcaaca tgcaggcaat
    25861 cgtcaatgcc gctattccca acgtccgcat tgccgccgtg ttgagcaaca gcgaaacggc
    25921 tgccggtttg caatgggcgg ccgaacgcgg catcccgacc gatagactga atcataaaaa
    25981 ctttacatcc cggcttgcct tcgataccgc catgatggaa aaaatcgacg catatcaacc
    26041 cgacttggtg gttttggcag gttttatgcg gattctgacc cccgagtttt gcgcccatta
    26101 cgaaggcagg ctgatgaaca tccacccgtc catccttccc tcgtttaccg gacttcatac
    26161 gcacgaacgc gctttggagg caggttgccg cgttgctggc tgtacgattc atttcgttac
    26221 ggccgagctg gattgcggcc cgattgtatc gcaagggatt gtgccgatac ttgatggtga
    26281 tacggcagac gatattgccg cccgggtttt ggctgtcgag cataaacttt atccgaaagc
    26341 tgttgccgat tttgccgccg gccgcctgat tatcgaggga aaccgcgtca gaaattcgga
    26401 aaacgccgat gccgcccgtt tcctgacggc gtaaaccggg cgggagcaaa tgatgaagac
    26461 ttttaaaaat atattttccg ccgccatttt gtccgccgcc ctgccgtgcg cgtatgcggc
    26521 agagctgccc caatccgccg tgctgcacta ttccggcagc tacggcattc ccgccacgat
    26581 gacatttgaa cgcagcggca atgcttacaa aatcgtttcg acgattaaag tgccgctata
    26641 caatatccgt ttcgagtccg gcggtacggt tgtcggcaat accctgcacc ctacctacta
    26701 taaagacata cgcaggggca aactgtatgc ggaagccaaa ttcgccgacg gcagcgtaac
    26761 ctacggcaaa gcgggcgaga gcaaaaccga gcaaagcccc aaggctatgg atttgttcac
    26821 gcttgcctgg cagttggcgg caaatgacgc gaaactcccc cccggggctg aaaatcacca
    26881 acggcaaaaa actttattcc gtcggcggtt tgaataaggc gggtacagga aaatacagca
    26941 taggcggcgt ggaaaccgaa gtcgtcaaat atcgggtgcg gcgcggcgac gatgcggtaa
    27001 tgtatttctt cgcaccgtcc ctgaacaata ttccggcaca aatcggctat accgacgacg
    27061 gcaaaaccta tacgctgaaa ctcaaatcgg tgcagatcaa tggccagtcc gccaaaccgt
    27121 aaatcgattt tcaggcgttt ttttgccgtt ttgtccatat ttctgcataa gccgcttgaa
    27181 attgtttaca ataacgccat atgcaaagcg gttttaacgc tattttcagg aatgacacca
    27241 tgcaggttac atcaaaatgg atagacggaa tgtgctttgt cggaacaacg gaaggcgggc
    27301 acagcgtcgt tatggagggg tcggcggcag aaggtaaggc taagcgcggg cccagccctt
    27361 tggaaatgct gctgttgggc gtggcgggct gttcgagcat cgatgtggtg atgattgccg
    27421 aaaaacagcg tcagaaagtg actgactgcc gtgcgacggt tacggcgaaa cgggcggacg
    27481 atgcgccgcg cgtgtttacc gaaatccaca tccatttcaa agtattcggg catgatttga
    27541 aagaatcggc cattgagcgc gccgttcaga tgtctgccga aaaatactgt tcggcttcga
    27601 ttatgttggg caaagcggca aagattaccc acagttttga aattgccggg gcagataaat
    27661 aggcatcggg cggcattatg gggaaacggc tgatgccgtc tgaagggaaa ggtgcggcag
    27721 gcaatccgga ggcgcataaa aaagcagata tattcggact gcacctcccg aatatatctg
    27781 cctgctgttt cctctttatt cagcctttat aatacttgga cttgtcgggg tattgtgcag
    27841 acttgattcc ggattgtcaa caattttcgg tcaaatttta aatgccgcgt tttaaaatga
    27901 tacccgcctg cttctgcggg cgggcgggag gcgagaatcc cgttatttta agccatatat
    27961 taatttattg aattatagtg gattaacaaa aaccagtacg
//
LOCUS       DAWFJR010000019        26520 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWFJR010000019 DAWFJR010000000
VERSION     DAWFJR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26520
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.019"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..794
                     /locus_tag="WGR04_000740"
     CDS             <1..794
                     /locus_tag="WGR04_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0794332.1"
                     /translation="SDPELEHSLITRIDFNLFCRFDELSIPDYSTLCRYRNWLAQDDT
                     LSELLELINRQLTEKGLKVEKASAAVVDATIIQTAGSKQRQAIEVDEEGQISGQTTPS
                     KDSDARWIKKNGLYKLGYKQHTRTDEEGYIEKLHITPANAHECTHLSPLLEGIAEGTT
                     VYADKGYDSAENRQHLKEHQLLDGIMRKACRNRPLTEAQTKRNRYLSKTRYVVEQSFG
                     TLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            944..1810
                     /locus_tag="WGR04_000741"
                     /pseudo
     CDS             944..1810
                     /locus_tag="WGR04_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981121.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1867..2940
                     /locus_tag="WGR04_000742"
     CDS             1867..2940
                     /locus_tag="WGR04_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0794333.1"
                     /translation="MRPFFVGAAVLAILGALVFFINPGAIVLHRQIFLELMLPAAYGG
                     FLTAALLDWTGFSGNLKPVATLMAALLLAASAILPFSPQTASFFVAAYWLVLLLFCAR
                     LIWLDRNTDNFALLMLLAAFTVFQTAYAVSGDLNLLRAQVHLNMAAVMFVSVRVSILL
                     GAEALKECRLKDPVFIPNVVYKNIAITFLLLHAAAELWLPAQTAGFTALAVGFILLAK
                     LRELHHHELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGIMGGV
                     MMVWLTAGLWHSGFTKLDYPKLCRIAVPILFAAAVSRAVLMNVNPIFFITVPAILTAA
                     VFVLYLLTFVPIFRANAFTDDPE"
     gene            complement(2987..5176)
                     /locus_tag="WGR04_000743"
     CDS             complement(2987..5176)
                     /locus_tag="WGR04_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ0794334.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILSIQTTFLDEPPLPESWRDLLSFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPLARFNKKAALWDALLSGGMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGKRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPYKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYVVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADALEFLNAAKETLAPLLPASVSQFGAAPMLMVRLA
                     ERERAQIFLESPSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            5315..6097
                     /locus_tag="WGR04_000744"
     CDS             5315..6097
                     /locus_tag="WGR04_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ0794335.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            complement(6416..8350)
                     /locus_tag="WGR04_000745"
     CDS             complement(6416..8350)
                     /locus_tag="WGR04_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ0794336.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVVLPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(8416..9594)
                     /locus_tag="WGR04_000746"
     CDS             complement(8416..9594)
                     /locus_tag="WGR04_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ0794337.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQASYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTTQTNTLNMEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIIQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAVEREIRTGMKDSMNTEVKSGLKEGDKVVISEMTADEQQESGVRALGGPPRR
                     "
     gene            9820..10209
                     /locus_tag="WGR04_000747"
     CDS             9820..10209
                     /locus_tag="WGR04_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0794338.1"
                     /translation="MKNVQKGFTLLELLIAVAILSILTLIAYPSYKAYTRRIRLSEVK
                     STLLMNAQNLERYYRQKGTFDNYDKNKLKQNKYFDITLSKVSPDHFTLQAAPNTATNE
                     GETCIVTLNDGGTLSAAGSGQDCPDFD"
     gene            10494..11540
                     /gene="adhP"
                     /locus_tag="WGR04_000748"
     CDS             10494..11540
                     /gene="adhP"
                     /locus_tag="WGR04_000748"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ0794339.1"
                     /translation="MKMQAVVVNKNVAGDVEIIEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVNADYAVKVPDGLDPAQASSITCAGVTCYKAVKV
                     SGVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(11705..15190)
                     /gene="smc"
                     /locus_tag="WGR04_000749"
     CDS             complement(11705..15190)
                     /gene="smc"
                     /locus_tag="WGR04_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ0794340.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEVSAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELGNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQMQRIRQE
                     QQQIRVQLEENELYVEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASDRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLQARSVPHGFVPPAPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELDNLAPELSTAEAAFKQAE
                     ATVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIFTLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIARLEQQTLDWQERQQELVLAYETEFQNDEQHIKLEELS
                     EAVQTLDEEYIVVQEKLAQIQEQGREQYAKVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITLLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            15439..16161
                     /gene="lpxH"
                     /locus_tag="WGR04_000750"
     CDS             15439..16161
                     /gene="lpxH"
                     /locus_tag="WGR04_000750"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ0794341.1"
                     /translation="MKPAYFISDLHLSEKQPELTALLLRFLRSSAARQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQNFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLATKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFGAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKY"
     gene            16430..18016
                     /locus_tag="WGR04_000751"
     CDS             16430..18016
                     /locus_tag="WGR04_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0794342.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(18185..18388)
                     /locus_tag="WGR04_000752"
     CDS             complement(18185..18388)
                     /locus_tag="WGR04_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ0794343.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPGNEQWNGHPRVFLPLGEGESG
                     SVACPYCGTRYRFDGKMPHHHYA"
     gene            18504..19001
                     /locus_tag="WGR04_000753"
     CDS             18504..19001
                     /locus_tag="WGR04_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ0794344.1"
                     /translation="MTALTLPEDIRQQEPSALLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLCRWKIKAVPKVLDKAKAL
                     YEQHGETIETLADEGRDIPSLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIRSNWETFA
                     HIGQA"
     gene            19083..20276
                     /locus_tag="WGR04_000754"
     CDS             19083..20276
                     /locus_tag="WGR04_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0794345.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PIVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLDAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLNRAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEATAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     ASQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(20679..21614)
                     /gene="hemC"
                     /locus_tag="WGR04_000755"
     CDS             complement(20679..21614)
                     /gene="hemC"
                     /locus_tag="WGR04_000755"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ0794346.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKVLYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYARLEEMPKGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHGVTNACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGAQELIGAVLNTEN"
     gene            21733..22248
                     /gene="ybeY"
                     /locus_tag="WGR04_000756"
     CDS             21733..22248
                     /gene="ybeY"
                     /locus_tag="WGR04_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ0794347.1"
                     /translation="MKRAKKYPFLTLQRQRFHLNFENASSAANLPSEHDFYRWAWSAL
                     KSGYRRADISLILLDEEAARAYNRDYRGKDYATNVLSFALNEGETLPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMRAA
                     GYPNPYREDGH"
     gene            22250..23074
                     /locus_tag="WGR04_000757"
     CDS             22250..23074
                     /locus_tag="WGR04_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEZ0794348.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(23203..24582)
                     /gene="nhaC"
                     /locus_tag="WGR04_000758"
     CDS             complement(23203..24582)
                     /gene="nhaC"
                     /locus_tag="WGR04_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ0794349.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVVLALMRINAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDRLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(24873..26096)
                     /locus_tag="WGR04_000759"
     CDS             complement(24873..26096)
                     /locus_tag="WGR04_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ0794350.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTGVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         6181 a         7561 c         6834 g         5944 t
ORIGIN      
        1 tctccgatcc cgaactcgaa cacagcctca ttacccgcat cgatttcaac ctgttttgcc
       61 gttttgacga actgagcatc cccgattaca gcaccttatg ccgctaccgc aactggctgg
      121 cgcaagacga caccctgtcc gaactgttgg aactgattaa ccgccaactg accgaaaaag
      181 gcttaaaagt agagaaagca tccgcagccg tcgttgatgc caccattatt cagaccgccg
      241 gcagcaaaca gcgtcaggcc atagaagttg acgaagaagg acaaatcagc ggccaaacca
      301 caccgagtaa ggacagcgat gcccgttgga taaagaaaaa cggcctctac aaactcggtt
      361 acaaacaaca tacccgtacc gatgaggaag gctatatcga gaaactgcac attactcccg
      421 ccaatgccca tgagtgcaca cacctgtcgc cgttgttgga agggatagct gaaggtacga
      481 ccgtctatgc cgacaaaggc tatgacagtg cggaaaaccg gcaacatctg aaagaacatc
      541 agttactgga cggcattatg cgcaaagcct gccgcaaccg tccgctgacg gaggcgcaaa
      601 ccaaacgcaa ccgatatttg tcgaagaccc gttatgtggt cgaacaaagc ttcggtacgt
      661 tacaccgcaa attccgctat gcgcgggcgg cctatttcgg actgattaaa gtgagtgcgc
      721 aaagccatct gaaggcgatg tgtttgaacc tgttgaaagc ggccaacagg ctaagtgcgc
      781 ccgctgccgc ctaaaaggcg gcccggatgc ctgattatcg ggtatccgga gaggattaag
      841 gggggatttg ggtagaatta ggcggtattt ggggcgaaaa cagccgaaaa cagctgaaaa
      901 cagctgaaaa cagctgaaaa cagctgaaaa cagctgaaaa cctgtgtttg ggtttcggct
      961 gtcgggaggg aaaggaattt tgcaaaggtc tcaggtcgca ctcgtcgtga cggaaagtac
     1021 cggcggtctc gaaatccccg ccgccaaagc catccgccga gcaggcatag ccgtgattat
     1081 cgccaacccg cgtcagacgc atcagtttgc ccaatcgcag ccgctgacca aaaccgacgc
     1141 caaagatgcc aaaatgcccg ccttcttcgc acagatgacg gcacagaaag aagattcgca
     1201 aaccatgccc taccaaccgc ccaccgaagc ggaagaagtg ttggaagcct tggttaaccg
     1261 ccgcaaccaa ccggcggata tgcggactgc cgagaaaaac cgtctgcatt aggttcatga
     1321 aacgcaagtc ggaagcgtca aacaactgat tgcccatttt gaccggctga ttgacgaatt
     1381 ggacaaacaa atcgacgacc acacccacac gcattttgac ggcaaagccc aagtggcgga
     1441 gcaaatcaaa ggcatcggtt cgataacgac ggctacgctg atggcgatgc tgcccgaatt
     1501 ggggcggctg tcgcacaaac ggatagcgag tttggccggc attgccccgc acccgaggga
     1561 gagcggggaa accaaattca aaagccgctg ctttggcgga aagtctgcgg tacgtaaggc
     1621 gctgtatatg gctaccgtgg cagcgacacg ttttgaaccg cttattcggg atttctacca
     1681 acgcctgccg tccgagggta agccgtataa ggttgccgtt acggcatgta tgcgcaaact
     1741 gctgacgata tcgaatgccc ggatgcgtga ttattttgcc gaaaacgata ccgccgaaaa
     1801 cggtatctaa acggcttgat ttgagttttg gtatttttgc ccgacggggt gaaaaataca
     1861 gttgctatgc ggccgttttt cgtcggcgcg gcggtgcttg ccatactcgg tgcgctggtg
     1921 tttttcatca accccggtgc catcgtcctg caccgccaaa tcttcttgga acttatgctg
     1981 ccggcggcat acggcggttt tttgactgcg gctttgttgg actggacggg tttttcgggt
     2041 aacctgaaac ctgtcgcgac tttgatggcg gcattattgc tcgccgcatc cgctatactg
     2101 cccttttcgc cgcaaactgc ctcgtttttc gtcgccgcct attggctggt gttgctgctg
     2161 ttctgcgccc ggctgatttg gctagaccga aacaccgaca acttcgccct gctaatgtta
     2221 cttgccgcgt tcactgtttt tcagacggca tatgccgtca gcggcgattt gaacctgttg
     2281 cgcgcgcaag tgcatctaaa tatggcggcg gtgatgttcg tatccgtgcg cgtcagtatt
     2341 cttttgggcg cggaagccct gaaagaatgc cgtctgaaag acccagtatt catccccaat
     2401 gtcgtctata aaaacatcgc cattactttc ctgctcttgc acgccgccgc cgaactttgg
     2461 ctgcccgcgc aaaccgccgg ttttaccgcg ctcgccgtcg gcttcatcct gctcgccaag
     2521 ctgcgtgagc ttcaccatca cgaactctta cgtaaacact acgtccgcac ttattacctg
     2581 ctccaactct ttgccgccgc aggctatttg tggacaggcg cggcgaaatt acaaaacctg
     2641 cccgcctccg cgcccctgca cctgattacc ctcggcggca taatgggcgg cgtgatgatg
     2701 gtatggctga ctgccggact gtggcacagc ggttttacca aactcgacta ccccaaactc
     2761 tgccgcatcg ccgtccccat cctcttcgcc gccgccgtct cgcgcgctgt tttaatgaac
     2821 gtgaacccga tattcttcat caccgtcccc gcgattctga ccgccgccgt gttcgtgctt
     2881 tacctgctga cattcgtacc gatctttcgg gcgaacgcgt ttacagacga tccggaataa
     2941 aaatgccgtc tgaaacggtt ttcagacggc atcgtattgc cgataatcaa gcctcctgcg
     3001 gatcgacatc caccgaccat ctaattttgc cgtcgcggtt ttgctgcaac acctgcaccc
     3061 acaaactcac ggcacggtgc aaatcctgtc gggacgggga ttcgaggaaa atttgcgcgc
     3121 gttcgcgttc ggcgaggcgc accatcagca tgggggcggc gccaaactgg gaaacgcttg
     3181 cgggcaacag cggggcgagg gtttctttgg cggcattgag aaattccagc gcatcggcaa
     3241 cacgcggcgc gtccgcgcgg acggcggtct gaaaaccgaa gggcggcatg gcgaacattt
     3301 gccgttcgtt caattcgttt tcggcaaaca cgacgtagtc ctgcgctttg acggcggcga
     3361 agacgggatg ttcgggcagt tgggtctgta tcagcacctt gccgggcttg tcggcgcgcc
     3421 ccgccctgcc ggacacctgc atcagctcgg caaacagcct ttccggcgcg cggaaatccg
     3481 cgctgtacag gctgccgtcg gcgttcaata cgataacgag gttgagccgc gcgaaatcat
     3541 gacctttggc gagcatctgc gtgccgacca gaatatcgat ttcgttgtcg gcgatgcggc
     3601 ggtacaaatc cgcccagtcg tttttgtgcg ccgtactgtc cctatcgacg cggacgacgg
     3661 ctgccttggg caggaaggcg cgcagggttt cttcgacgcg ctgcgtgccg tggccgacgg
     3721 cggtcaggtc ttggttgccg cagtcggggc atttgtacgg gatgggttcg cggtggtcgc
     3781 agtggtggca gcgcagttgg cgggcgcgtt ggtgcagcac cattttggcg gagcagttcg
     3841 ggcagccgaa ggtatgaccg cagtcgccgc aaaacagcgc gggcgcgaag ccgcgccggt
     3901 taaggtacac cagcgacata ccgcctgctt caaagttctg tttcaaaagc tgcaaggctt
     3961 gcggcgagaa gccgttgtcg agtttcagac ggcctacgtt gagaatgtcc acttgcggca
     4021 gttgcgcggc ggtatgggcg cgttcggtca gttgcagcag gcggtacgcg ccactttgcg
     4081 ccttgtgcca gctctccaag ctgggcgtgg cactgcccaa cacgacgggg cagccgccct
     4141 gcttcgcccg ccacaccgcc aaatcgcggg catgatagcg caattcgttg tcttgtttga
     4201 acgagccgtc gtgttcctca tcgaccacaa tcagcccgac atcatccatc ggcgtgaaca
     4261 ctgccagccg cgtgccgatc accagcttcg cctgccctaa catcgcgcgc agataatcct
     4321 gcgtgcgctt gcctgccgcc atctgactgt gcaacacggc ggtcggcacg tcggcaaaac
     4381 ggttttccac ccgcttcaaa agctgcggcg tgaggttgat ttcgggcaac agaaacaaca
     4441 cctgccgccc ctgagccaac actttcgcca tcgcatcaaa atacacctcg gtcttgccgc
     4501 tgccggtgat gccgtacagc agaaacggct ggaagcgtcc caatgccgtc tgaatggcat
     4561 cggaagcctt ctgctggtca gcatttaata caaattcaga gtgcgaagcc tgcccattac
     4621 acgaccttaa aacgggcttc gccgcttccg tcgtttcaat ccagccttgt tcagcccaat
     4681 cctcaatcaa tttcgccgcc tgcgcgttta cctgcttcaa cgccgccatc gtcatcccgc
     4741 ccgacagcag tgcgtcccac agagccgctt ttttgttgaa ccgcgccagc ggcggcgttt
     4801 gcgccctgcc cgcttcgttc agcgcgtaaa acaacggcgg ctgcggcatt tccaccgcgc
     4861 gcgtttcctt caaaccctgc ggcagcgcgg caaacaccgc ctgccccgtc ggatagtggt
     4921 aataacgcga cgtaaacgag agcaaatcac gccagctttc aggcaacggc ggctcgtcta
     4981 aaaaggtcgt ctgaatactc aaaatccgcg ccgtatccat atccggcgca atatccgttt
     5041 cccacaccat cccgaccacg gttttattgc ggaaaggcac aagcacccgc gttcccggag
     5101 gaagcggatc ggaatgggaa taagtcaaaa ggccgtctga aagcggcacg tttacggcga
     5161 tgcggtggta gatcatacag gcaggcaaat catttttttg cgtattttaa caggcggaag
     5221 ctattttcag acgataaatt gagcctaggc gcaatgcagg cgcaacagag atttgataca
     5281 attccgcttt tccaacacgc gcaatcatct ttatatgaaa accaagttaa tcaaaatctt
     5341 gacccccttt accgtcctcc cactgctggc ttgcgggcaa acgcccgttt ccaatgccaa
     5401 cgccgaaccc gccgtcaaag ccgagtccgc cggcaaatcc gttgccgcct ctttgaaagc
     5461 gcgtttggaa aaaacctatt ccgcccaaga tttgaaagtg ttgagcgtca gcgaaacacc
     5521 ggtcaaaggc atttacgaag tcgtcgtcag cggcaggcag attatctaca ccgatgccga
     5581 aggcggctat atgttcgtcg gcgaactcat caacatcgac acgcgcaaaa acctgaccga
     5641 agaacgcgcc gccgatttga acaaaatcga cttcgcctcc ctgcctttgg acaaagccat
     5701 caaagaagtg cgcggcaacg gcaagctgaa agtcgccgtc ttctccgacc ccgattgtcc
     5761 gttctgcaaa cgcttggaac acgagtttga aaaaatgacc gacgtgacgg tttacagctt
     5821 tatgatgccc attgccggcc tgcaccccga tgccgcgcgc aaggcgcaaa tcttatggtg
     5881 tcagcccgac cgcgccaaag cgtggacgga ttggatgcgt aaaggcaaat tcccggtcgg
     5941 cggcagcatc tgcgacaatc ccgtcgcgga aaccacttcc ttgggcgaac aattcggctt
     6001 caacggcacg ccgaccctcg tcttccccaa cgggcgcagc caaagcggct acagcccgat
     6061 gccccaactg gaggaaatca tccgcaaaaa tcaataatcc atccgcaaaa caaaaggctt
     6121 ggaaaattcc aagccttttt tgcgggaaat gccgtctgaa aaccaatccg cactgaaagt
     6181 tatagtggat taaatttata gtggattaaa tttaaaccag tacagcaagc caacaaacac
     6241 atcctaaccc attgaatgat tgaaaataat aaactgaaca atacgaaaca caccgccgtc
     6301 attcccacgc aagtgggaat ctagaacgta gaatctaaga aaccgttttt gtccaataag
     6361 tttctgctcc aaaatgaccg gtcctgcagc ttcagacggc atctttgtgc caacctcaat
     6421 cctgcgccaa tgcgtctatc ggattgagtt tggctgcttt attggcaggc ataaagccga
     6481 acgcgatgcc gattccggtc gaacaggcga ccgccccgat aacggatgcc gccgaaatgt
     6541 ccatcgggaa gtcggttaca aaatgattga acacgaggct gacggcggcg gacaaaccca
     6601 cgccgaccaa accgccgatg acgcagatta acaccgcctc aatcaaaaac tgctgcaaga
     6661 tattgccgcg ccgcgcgccg attgccatcc gtatgccgat ttctttggtg cgctcggtaa
     6721 cggacaccag catgatgttc atcacgccga tgccgccgac taccaatgaa atcagggcga
     6781 tggaggaaat cagcagcttc atcgtaccgg tggtgctttc gactatctgc ctgatgctgt
     6841 cgctgttgtt catgaagaaa tcttccgtgc cgtgccgcgc tttgagcaga tcggtcagcc
     6901 ctttttcggc aacctgggta ttggcattgt ctttgatttt gacggtgatg gagttggtgt
     6961 ggctctcgcc tgtgatttgg tgcatcaccg tcgtataggg cgaccaaagc atcagcacgt
     7021 cggaattgcc gaaagcgttt tcgtcttttt tcatcacgcc gatgacggtc aaggggcgtt
     7081 tcctgaacaa aatggtttta cccaacggat ccgagtccgc aaagagtttg tctttgacat
     7141 tttggtcgat gacgacgacc tgcgcgtctt ctttcacatc gttttcgtca aacagccgcc
     7201 ccgtttccag cttcagtccg cgcacgtcga aatattgttc gcccacgccg tacaaagaag
     7261 cggtcaggtc ggtattgcgg taagtcagcg tgccgccgct cgaagtcatg ggcgtggcgg
     7321 aagcaacgta gctttgtttg gcgatgattt ttgcgtcgtc tatggtcagg gttttaatcc
     7381 tgccgctgcg cctgtcgccg aagccgcgcc ccgggaagat gctgatggtg ttcgtcccta
     7441 tcgaactgat gtcttcaagg atttttttct gcgaaccatt gcccaatgcg acgacggaga
     7501 caaccgaagc gataccgatg atgatgccga gcatcgtcag aagcgaacgc attttgtgcg
     7561 ccaatactgc ttgcaccgac attctgaagg cttcgacaaa ctggtcgtaa taaaacgacc
     7621 acgaggcttt ttcctgaatc ctcccgacat tgcttgcggg gatttctgga tttttcgagg
     7681 tgtcggaaat gatttcgccg tcccggattt cgatgacgcg gttggcattg gcggcgatgc
     7741 cggggtcgtg cgtgaccata atgacggtat gcccggcttc gtgcagctta tggatgattt
     7801 ccatcacgtt tttgccgctg gcggtatcga gcgcgccggt cggctcgtcg gcgaagatga
     7861 tttctccgcc gttcatcagg gcgcgggcga tggagacgcg ctgctgctgt ccgcccgaga
     7921 gttcgccggg cttgttgccc tctttgcttg ccaaacccaa atcctgcaag agtttgtccg
     7981 cccgcgcgga acgctctttg ccgcccgcgc ccatatagac ggctggcagc acgacgttat
     8041 cccttgcggt cagcgagctt aagaggttgt agcgttggaa gataaagccg aagcgttcgc
     8101 ggcgcaatgc cgccagttcg tcaggctgca ttttggcagt ttcgatgccg tcgattcggt
     8161 acgaaccgga accggcggta tccaaacaac cgagtatgtt catcagcgtg gacttgcccg
     8221 aaccggactg cccgatgatg gcgacaaaat cgcccttctc tatcgacagg ctgatgtctt
     8281 tcaaaatatg gacgcggttc tcgccgctgc cgaaatagcg gttgatgttt ttacattcga
     8341 tcaagctcat aatattccgt aaatcaataa caaatgccgt ctgaaaccgt ttccgtgttc
     8401 agacggcata ttcgtttatc ggcgcggcgg gccgcctagg gcgcgcacgc cgctttcctg
     8461 ctgttcgtcc gccgtcattt cggagataac cactttgtcc ccctctttca aaccgctttt
     8521 cacttcggta ttcatactgt ctttcatacc ggtccggatt tcgcgttcca ctgctttgcc
     8581 gtctgcgccc agcacgcgca caaacgcctt gccgccgcga tttttcacgg tcagtgacgg
     8641 aataatcagc acatttttca cgccgtcgat ttcaaccgta ttctgcgtcg tcatccccgt
     8701 ggcgagtttg ccgtccggat tcggcacaaa cgaacgggca taataataga ccgcattgga
     8761 agccgtatcc gtactgctgt tgtagccgcc cgacgacatc gtggtcagcc cggggtcgac
     8821 gctgtcgagt tttgccttaa tcggcgtatc cggttcggac aaaatcgtaa acgaaatatc
     8881 ctgccccgcc ttcaccttgg taatatcgcc ctcggcaatc tgcattttgt tcaacatcat
     8941 atccaaattg gcaagctgga taatcgtcgg cgtagactgc gccgcgttca cagtctgccc
     9001 ctcttccacg agaatcgcca ccaccgtgcc gtccatcgtc gcggtaatgc gcgtgtagcc
     9061 caattccgac tcggcggtat tgatggaaat tttgctctgt ctgattaaag ccttcagttc
     9121 ggcaacgttt gccttggcgg cggcaaacgc atcctgcgcg ctttccaaat cttctttaga
     9181 ggttgcatca tccttccata acgccgcctg acgcttatat ttcttctccg cgctgcccaa
     9241 tgcaatctgt gccgacacca gcttcgcctg atacgtttcc aatttggatt tttccatatt
     9301 gagcgtgtta gtctgcgtgg tcgaattgat ttccgcaatc aaatcgccct ttttgacctg
     9361 ttgtccgagt ttgacataaa gtttcttaat ctgccccgat gcctgcgcgc cgaccgatac
     9421 cagattcgac ggcgaaatct cccccgtcgc ggaaaccgtc cggctgatgt cgccgcgcct
     9481 gaccgtttcc gtaatataag aagcctgcgg ttcgggcttc agataagacc atccgcccca
     9541 aaccgctgcc gccgcgactg ccgcaacagc cgcccatttc atcatttttg ccatattttc
     9601 aattctctgt atgcgtcaat cccataaatc ggatgcggat tctaccgcaa gaccctgccc
     9661 catccaaggc ggcaaactgc cgacaataaa aataaccatt tgttttaaat ggcttataca
     9721 atctgctttc ccgaaaattt caatctgttt acatattccg ccatttccga tacggcgtta
     9781 taatgccgcc gaccgataaa caaatggaaa cacattgcta tgaaaaacgt tcaaaaaggc
     9841 ttcacgctgc tcgagctgct gattgccgtc gccatcctca gcatcctgac gctcatcgcc
     9901 tacccttctt ataaagccta cacacggcgc atccgcctgt cggaagtcaa atccaccctg
     9961 ctgatgaacg cgcaaaactt ggagcgttac taccgccaaa aagggacgtt tgacaactac
    10021 gacaaaaaca aactgaaaca aaacaaatat ttcgacatta ccttaagcaa agtcagcccc
    10081 gaccacttca cccttcaggc cgcccccaat acggcgacca acgaaggcga aacctgcatc
    10141 gttacgctca acgacggcgg cacactgtcc gccgccggca gcgggcagga ctgcccggac
    10201 ttcgactaat cctccgccga agcccgcaaa cccgtaaaaa tgccgtccgg acacagcttc
    10261 agacggcatc tttacgggtt tgccatccgt ttttttcaaa cttgaaacct gccgcccccg
    10321 catccggcaa ccgcccccga tgccgcacct ccccaactgt tataatataa catataaccc
    10381 atatttttca acaacatacc atttgacccg caagatgcgg aagcgtatgc ttacccttat
    10441 atcggacaac attccgttta caggtttttt taccgtaact aaggagcgca atcatgaaaa
    10501 tgcaggcagt tgttgtgaat aaaaatgtag cgggcgatgt ggaaataatc gaacgcgagg
    10561 ttcgcccgtt ggaatacggc gaggcattgg tcgaagtcga atattgcggc gtgtgccaca
    10621 ccgacctgca cgttgcggca ggcgactacg gcgaaaaacc gggccgcgtg ttgggacacg
    10681 aaggcatcgg tttggttaaa gaagttgccg acggtgtgaa aaatctgaaa gtcggcgacc
    10741 gcgtcagcat cgcctggctg ttccaaagct gcggctcttg cgaatactgc aataccggcc
    10801 gcgaaaccct gtgccgttcc gtattgaacg cgggctacac cgccgacggc ggtatggcga
    10861 cccactgcat cgtcaatgcg gattatgcag tcaaagtccc cgacggtctc gaccccgccc
    10921 aagcgtccag catcacttgc gccggcgtta cctgctacaa agccgtgaaa gtgtccggcg
    10981 tgcgtccggg acagtggatt gccatctacg gcgcgggcgg tttgggcaac ttgggggtcc
    11041 aatacgcgaa aaaagtattc ggcgcacacg ttgtcgccat cgacatcaac gacgacaaac
    11101 tggcgtttgc caaagaaacc ggcgcggatt tggttgtcaa cgccgccaaa gaggacgctg
    11161 ccaaagtgat tcaggaaaaa acaggcggcg cacacgctgc ggtcgtaacc gccgtatctg
    11221 ctgccgcatt caactctgcc gtgaattgcg tccgcgcggg cggacgtgtg gttgccatcg
    11281 gactgccgcc ggaatcgatg gatttgtcca tcccgcgttt ggttttggac ggcatcgaag
    11341 tggtcggctc tttggtcggc acgcgcaaag atttggaaga agccttccaa ttcggcgcgg
    11401 aaggtttggt tgtgccgaaa gtccaactgc gtgctttgga tgaagcaccc gccattttcc
    11461 aagaaatgcg cgaaggcaaa atcaccggtc gtatggtgat cgatatgaaa aaagaatgcg
    11521 gctgcggcca tcaccactga tttgacgtgg cagtacacat cgaaatgccg tctgaacgct
    11581 gttcagacgg cattttttat ggatttggat ttgattttaa tctgttctgt ttgaaatacc
    11641 gtctgaaaac ccttatcgca acacgtcatt aaaaacggca agattcagcc gttctgcaac
    11701 ccgttcaaac ggcttccgcc atttccaacg cctgcttgat gtccacggcg acgacgcgcg
    11761 atacgccttt ttcctgcatg gttacaccga ccagctgttc cgccatttcc atggtcaggc
    11821 ggttgtgtga gatgtagagg aattgggttt gcgccgacat ttctttgacc aaattgcaga
    11881 agcgcgaggt gttggcatcg tccagcgggg catcgacttc gtccaaaagg cagaacggag
    11941 cagggttgag gctgaacagg gcgaacacga ggctcatggc ggtgagggct ttttcgccgc
    12001 cggaaaggag gtggatggtg ctgtttttct tgccgggcgg gcgcgccatg atggacacgc
    12061 cggcggtcag gaggtcgtcg cctatcattt tgagggtagc ttcgccaccg ccgaacaggg
    12121 tcgggaagaa agtttggact ttgccgttga cggcatcgaa ggtttctttg aaacgcgctt
    12181 tggttttgtc gtcgatttgg gcgatggctt cttccaaaag ggtgatggct gcctgcacgt
    12241 cttcgctttg gctgcggtag tagccgtcgc gttcgcgcgc ttcttcgagt tcttgcaggg
    12301 cggcgaggtt gaccgcgccg agtgcttcga tttgttgcga aaggctgccg atgctgctgt
    12361 tcaatacttt cggcgattct ttcgccaacg cttcgagcgc gtccaaatcg gcggcgcgtt
    12421 cggtcaggtt ttgatggtag cgtttggcgt tgatcagggc ttcctgctgc tgcaacaagg
    12481 cggtttgggt ggcggcctga agctgcggca gcttggtttg cagggtttgc actttagcgt
    12541 attgctccct gccctgttcc tgaatctgcg cgagtttctc ttgcacaaca atatattctt
    12601 cgtccaaggt ctgtacggct tcgcttaatt cttcaagctt gatgtgctgc tcgtcgtttt
    12661 ggaactcggt ttcataggcg aggacaagct cttgctggcg ttcctgccag tctagggttt
    12721 gctgttcgag tcgggcaatt tgctgccggt aattttgttt ttgctggttg agcttatgga
    12781 cggcgacttc ggccagcccg tattggcggt tggcttccaa cagggcaagc tgcgcctgtt
    12841 tcagacggcc ttgctgctct tggcggctgt gcgcggtggt ttgctgttgg tgttcgagtt
    12901 cggcggcggc ttcctgcaaa gtaaagatgt cgtctgaaag cccgtcggac gtgtgttgca
    12961 acacggtctg ctcttccgcc aactgcgcca gttcgcgctc gatgtgttcg cggcggattt
    13021 gcccttggtt ggtacgcgcc agaagttcgg cggcgcgttg ctgcgcctga ctgtactggc
    13081 gcgtgtgctg ctgttgctgc tgcatcaggt ttttatgttg cacttcggac gagcgtacag
    13141 ttgcctccgc ctgtttgaac gcggcttcgg cggtggagag ttcgggggcg aggttgtcca
    13201 gttcggacgc gatgccgtcg agacgggctt tttgggcaat cagactttcc tgcgcgggtt
    13261 tggcatagag caggacgctg actttatcga cctgatggcc ttcgggcgtg agccagattt
    13321 ggtgtgcgcc caaatcgctt tgatgcgcga gggcgtaact caaatcaggc gcacacaata
    13381 cgccgtcgag ccagtggtgc aatgccgtct gaaacggcgg ctgcgcttgg atttggttca
    13441 gcaatgcctg tacgggcagg gattttttga tgccgccgga gagaccgtct gaaagccacg
    13501 ccgcctgccc ctgcggcaaa ggcgcggggg gaacgaaacc atgcggtacg ctacgggctt
    13561 gcaggcgttc ggcaaggatg acggacaagg cgtgctgcca ctcggcgggc gcggtgatgt
    13621 gttgccacag ttgcggcgcg gcggcgtggt cggttgcctg ccagaaatcg gcggcttcct
    13681 gctgttgcga caggatttgc gacaacgcct gctgctgcgc ctgcaaggtg atgtgttgct
    13741 gcttcaggct ttggaagcgg tctgaggccg tctgaaacgc ctcgcgggca gcgtgtaagg
    13801 cttcttcggc ggcaatgatt tgttcttcgt aatgctcttg ccggctttgc aacaaggcgg
    13861 cggcttcctg cgcggcggcg gtttcggctt cgtcgggcag gttcagggct tggttttcct
    13921 gtttcagacg acctttgcgc tcttcgtgct tggcaacggt ttgttcggca tgggcaagct
    13981 gctgctgctt caacgccagt tcgcggcgga tgcggtttgc ctcgtcctgc tgggtttgga
    14041 aggcggcgtt gagcgtagct tgggcttctt ccaattcggg cagacgctct tcgtgttcgg
    14101 caacctgcat cgcccattcc gccagctcgg tttgcttttc ttcgacgtat aactcgtttt
    14161 cttccagctg cacgcggatt tgctgctgct cctgccggat gcgttgcatc tgcgcctgcg
    14221 ccgcctgctt gtcgcgttcg atgcgttggt gcaggttttg ctgatggcgg atttgttctt
    14281 ccaaacgggc aatctgctcg cgcaacacac cgcgtttgtt gcccagttcg tgtaccgcct
    14341 gctgctgcga ctgttcggcg gtctgcaagg cgtgtacttc gtcgtttaac gcctgaacct
    14401 gcgcggcggt ttcgtcctgc tgcgcctgca aagactgatg ctgcgcggtc gccttgtcgg
    14461 cggcggcaag cgattgctgc cattgggcgt aatcgagcaa atcctgttgc tggttcagct
    14521 gcgcggtcag ggatttgtag cgttcggcgg tttccgcctg tttctccagc ttttccacct
    14581 gacgcgctaa ctcgttctgc aaatcgccca aacgctgcaa atgctcgcgc gtgtctttca
    14641 gacggccttc cgtctcctta cggcgttcct tatatttgga cacgcccgcc gcttcctcga
    14701 tataggcgcg caattcctcc ggccgcgctt cgatgatgcg cgaaatcatc ccctgctcga
    14761 taacggcata accgcgcgcg cccacgcccg tacccaaaaa caaatcggta atgtcgcggc
    14821 ggcgcacggt ctgattgttg atgaaataag tcgattcgcc ctgacgcgtc agctgccgct
    14881 tgatgctcac ctcggcatac tgcccccacg ccccctgcaa actgtggtcg ctgttgtcaa
    14941 ataccagctc caccgaagcc ctcggcgcag gacggcgcgt cgccgcaccg ttaaaaatca
    15001 cgtcctgcat actctcgccg cgaagctgct tcgccgaaac ctcacccaac acccagcgca
    15061 ccgcgtcaat cacattcgac ttgccgcaac cgttcggccc gataaccgcg acaagctgcc
    15121 ccggaacatg aatcgtggtc gggtcggtaa aagatttgaa gccggagagt ttgatgtggg
    15181 tcaggcgcat aatggtcgga aaaaataaaa aagaagcgta ttttaacgga aatcccgccg
    15241 caccacccca tatcttgccg gcaaaacctt accgcatccc gcacccgcga tgccgtctga
    15301 agccttcaga cggcattttt aacgcgcccg aaccccggtt ccgccaatgc ccgatgcttg
    15361 gcgcggcatt tgccgtattg ccccaccgcc gggcgcgtta taatccgccc cgacatccgc
    15421 ccgattggaa acctgcctat gaaacccgcc tatttcattt ccgacctgca tttgagcgaa
    15481 aagcagcccg aactgaccgc gctgttgctg cgttttttac gttcttccgc cgccaggcag
    15541 gcgcgggcgg tttacatttt gggcgatttg tttgattttt gggtgggcga tgacgaagtt
    15601 tccgagttga atacttcggt tgcgcgtgaa atcaggaaat tgtccgacaa aggcgttgcc
    15661 gtgttcttcg tcaggggcaa ccgcgacttc ctgatcggtc agaatttttg ccggcaggcg
    15721 ggcatgacgc tgctgccgga ttactcggtt ttggacttgt tcggctgcaa aaccctcatc
    15781 tgccacggcg acactctgtg tacagatgac agggcatacc aacgtttccg caaaatcgtg
    15841 catcggaagc ggctgcaaaa actgttccta atgctgcccc tgaagtggcg cacgcgcctt
    15901 gccaccaaaa tcaggcgtgt cagcaaaatg gaaaaacagg tcaagcccgc cgatattatg
    15961 gatgtcaatg ccgcctttac cgcgcggcag gtacgcgcct tcggtgcgga aaggctgata
    16021 cacggacaca cccaccgcga gcatatccat cacgaaaacg gctttacccg catcgttttg
    16081 ggcgactggc ataacgacta tgcttcaatc ctccgcgtgg acggggacgg cgcggtattc
    16141 gtgccgctgg aaaaatactg aaaatgccgt ctgaagcctt tcgggcggct ttttttacac
    16201 gcccctttcc cacactccct ccgccgcatc atcaccatcc ttaacccgca ttaatagcca
    16261 ttatcgataa aatgcttgac cgattacata agattacgta aagtgtgcaa acgcataata
    16321 attggtctta ccaatttatc ttttaaccaa aatatcgggt gtacgggatt tgaacgtctt
    16381 tccaaacctc gacgcgcctt atgatgcaaa tgcctaaaaa ggattcgtta tggcactttt
    16441 cctcagcata ttccccatcg tcctgctgat ttggctgatg gtgaaaaaaa acagtatgcc
    16501 ctcctacgtc gcgctgccga ttaccgcagt gctgatttac gccatcaaac ttttctactt
    16561 cgacgatgcg ggaatgctgc tcaacgccac cgccgcttcc ggcctcgtca aaacgctcac
    16621 gccgattacc gtgattttcg gcgcgattat gttcaaccgt atgatggaaa ccacgggctg
    16681 catcgatgtc atccgcaaat ggctggcgac catcagcccc aaccccgtag cgcaactgat
    16741 gattatcggc tgggcttttg cctttatgat tgaaggcgca tccggcttcg gcacgcctgc
    16801 cgcgattgcc gcgccgattc tgatgagctt gggcttcaac ccgttgaaag tggcgatttt
    16861 cactttggtg atgaactccg tccccgtctc tttcggcgcg gtcggtacgc cgacctggtt
    16921 cggtttcgca ccgctgaacc tgagtgccga agacatcctc gccatcggca ggcagaccgg
    16981 cgtaatgcac ttcttcgcag gtttcgtcat ccccgtcatc ggcttgggct tcatcgtacc
    17041 ttggtctgaa atccgcaaaa acttgggctt cgtcgccatt gccgtcttct cctgcaccat
    17101 tccttatgtc gcattggcga tggtcaacga agaattcccg tcgctcgtcg ccggcgcaat
    17161 cggcctgatg gtgtccgtat tcgccgccaa ccaaggctgg ggcttgagca aagaccacgc
    17221 caaagacccg aatgccgaaa aagtgccgtt cgcccaagtc gccaaagcac tcgccccttt
    17281 gggtatgctg atcggcatgc tggtggttac gcgcatcaaa cagctcggca tcaaaggcat
    17341 tttgaccagc aaagaagaat ggttcagctt ccaactgccg tttgatttgt ccaaaatcac
    17401 cgtcagcgac tccctgacga ttaccttcgg caatattttc ggacaagatg tcagcgcgtc
    17461 ttaccaaacg ctgtacgtcc cggcttggat tccgtttgtg ctgaccgttt ggatttgcat
    17521 cctgctgtat aaaaccaaat tcaaagatgc ctggacgatt tatgccgtaa ctttcaatca
    17581 aaccaaaaaa ccgctgcttg ccctgatggg cgcgctgatt atggttcagc tgatgctggt
    17641 cggcggcgac aattcgatgg tgaaaatcat cggtaaggaa tttgccgcaa tggcgggcga
    17701 acactgggtt tatttctcgc cgtatctggg cgcgatcggt gcgttcttct ccggctccaa
    17761 caccgtgtcc aacctgacct tcggaccgat tcagcagcaa atcgccttgg ataccggcct
    17821 gtccgttacc ctgattctgg cgttgcagtc cgtcggcggc gcgatgggca atatggtgtg
    17881 cctcaacaac atcatcgccg tatgtaccgt attggatgtg aaaaattccg aaggtgcgat
    17941 tatcaagaaa accgttatcc cgatggcgat ttacggcgtg attgccgtcg tcgcggcaat
    18001 gattttcttc ctctaggcga aacgccacca tccatagcgg cagcacgcgg ggaacggaaa
    18061 ccgccgttcc ccgtctgggc ggcgcgtcgg gcagaatccc ggtgccggaa caagcccgcc
    18121 ccgccaaaca aatgccgtct gaaaccgaaa aaggcttcag acggcatttt tccgccgctt
    18181 gcgttcaggc gtaatgatga tgcggcatct tgccgtcaaa gcggtagcgc gtgccgcaat
    18241 acgggcaggc aacgctgccc gattctcctt cgcccaaagg caaaaacact ctcggatgcc
    18301 cgttccactg ctcgttgccg ggtcccgagc aatacagcgg cagattttcc ggcaacacgg
    18361 aaatttcctg cagattcaga ttgtccattt gattttcctt tgcgtggttg gcgtgcgccc
    18421 attttacgcc atcggcaggc taaaggatat tttcggcgca aagccgcaat ccgctataat
    18481 ccccactttt cagacggcat accatgactg cgcttaccct tccggaagac atccgccaac
    18541 aagagccatc cgccctgctc tataccctcg tttccgccta cctcgaacac accgcccaaa
    18601 ccggcgacga atccctctcc tgcctgtccg acgaccagca cacgctgacc gcattctgct
    18661 acctcgacag ccaagtcgaa gaaggcggct tcgtgcaact catcgcctcc ggttacggcg
    18721 aatatatttt ccgcaacccg cttgccgaca gcctgtgccg ctggaaaatc aaagccgtgc
    18781 cgaaagtctt ggacaaagcc aaagccctct acgaacaaca cggcgaaacc atcgaaacgc
    18841 ttgccgacga agggcgggac atcccttccc tgcgcaaaca gttccccgac tttgaagaat
    18901 gggacggcgc atactacgaa gccgccgaac aagacctgcc cctgcttgca gaacacatac
    18961 ggtcaaactg ggaaaccttc gcccatatcg ggcaggcgtg attgcgtctg tttccagccc
    19021 gtgtaaaaca gcgtaaaatc ggcaaacccg aatgaccttc cgtccccatc aaggagcaag
    19081 ctatgttctt caagcacatc gaagccgccc ccgccgatcc gattctcggt ttgggcgaag
    19141 cgttcaaagc cgaaacccgc cccgaaaaag tcaacctcgg catcggcgtg tacaaagacg
    19201 catccggcgc gacacctatt gtcaaagccg tcaaagaggc tgaaaaacgc ctgttggaaa
    19261 gcgaaaccac caaaaactac ctcaccatcg acggtgttgc cgactacaac gcgcaaaccc
    19321 aaatcctgct gtttggcaaa gaccacgaaa tcatcgccag ccgccgcgcc aaaacagcgc
    19381 aaagcctcgg cggtacgggc gcattgcgta ttgcggccga gtttgccaaa cgccagttgg
    19441 acgcgcaaac catctggatt tccaatccga cctggccaaa ccacaacgcc atcgccaagg
    19501 cggtcggtat ccaagacaaa ccttatcgtt attatgatgc cgccaaacac ggtttggatt
    19561 gggacggtat gattgaagac ttgaaccggg cgcaaaaagg cgacatcgtc ctgctgcacg
    19621 gctgctgcca caaccctacc ggcatcgacc ctacgcccga acaatgggaa actctggcaa
    19681 aactttctgc cgaaaaaggc tggttgccgc tgtttgactt tgcctaccaa ggcttcggca
    19741 acggtctgga agaagatgcc tacggcttgc gcgtgttctt gaaacacaat acagaattgc
    19801 tgattgccag ctcttattcc aaaaacttcg gtatgtacaa cgagcgcgtg ggcgcgttca
    19861 ctttggtggc cgaagatgaa gcaaccgccg cccgcgccca cagccaagtc aaaaccatca
    19921 tccgtacctt gtattccaat ccggcttcac acggcgcgaa cactattgcg ctggtgttga
    19981 aaaatgatga tttgaaagca caatggattg ccgaactcga tgaaatgcgc ggccgcatca
    20041 aagccatgcg ccaaaaattt gtcgagttgc tcaaagccaa aggtgcaagc caagactttg
    20101 atttcattat cgaacaaaac ggtatgttct ctttcagcgg tctgaccccc gaacaagttg
    20161 accgtttgaa aaacgagttt gccatttatg ccgtccgctc cggccgcatc aacgtcgccg
    20221 gcattaccga cgacaacatc gattatctgt gcgaaagcat cgtgaaagtc ttgtaaaaca
    20281 aaagcgcata cgaaatgccg tctgaacctt aaccagttca gacggcatct gttttttgtt
    20341 aaaaaacaat aaaccataac aatttcaaac aagctaaaaa catctataaa atcaacccaa
    20401 tatgatgaac tcaaataaaa aatacgaata tcatcattct caaattcaaa atttgaacgc
    20461 cacagtatta tccgaagcca caaaaacctt tccgccgtca ttcccgcgca ggcgggaatc
    20521 tgggaaccca atgctgcaag aattgattag aaacaactga aaccgagcaa actggattcc
    20581 cgcctgcgcg ggaatgacga aataaggcga gtaaaactga tgaaacaaat tgattaaaaa
    20641 caataattta tattatttta tttaaatttt cgataaaatc aattttccgt attcaatact
    20701 gctccaatca attcctgcgc accgtcgtcc gccaatttct tagcgacagc gcgtccgagc
    20761 gcgtcggcat attcggcagg ggcttgcgcg tccgcccgca acacaaccga accgtcgggg
    20821 tgtccgacca agccgcgcaa ggtcagcaag ccgttttctt ccgtgcaata tgcggccaaa
    20881 ggcacttggc agcttccgcc caaagcgcgt gcgagggcgc gttcggcggt aacgcaggca
    20941 ttggtaacgc cgtggttcaa gggtttcaaa acttcataca aatcttcgcg gtgcgcggca
    21001 atttcgatac ccaatgcgcc ttgtccggcg gcaggcaggc tgtcggattc cgacaaaatc
    21061 atgcggatgc gtccgtccaa tttcagacgc tgcaaaccgg cggcagccaa gataattgcg
    21121 tcgtattcgc cgttatcgag tttggacaaa cgggtttgca cattgccgcg caaaggtttg
    21181 ataagcaaat gcggatagcg cgcacgcaac tgggcttcgc ggcgcaggct ggatgtaccg
    21241 acgacagcgc ctttgggcat ttcttccaaa cgcgcgtatt ggttggacac aaacgcgtca
    21301 aacggattgg cgcgttcgcc gatggccgca agcgcgaaac cttcaggcaa atccatcggt
    21361 acgtctttaa tcgaatgcac cgccaaatcg gcgcgcccgt catataaagc ctgttccaac
    21421 tctttgacaa acaagccttt accgccgact tttgacaaag ttttgtccaa aatctgatcg
    21481 ccgcgcgtgg tcatgcccaa aatctcgact tcgcaatcgg gatacaggac tttcagacgg
    21541 ccttggatat gctttgcctg ccacatagca agcaggcttt cgcggctggc gataacaagt
    21601 tttttcgggt tcatgttcaa tgcgttcaaa gaatggaaac ggcgtggagt ctatcatatt
    21661 ttgaacgttt cttcagacgg catcatcagg tctatgttat atttacgccc acaaaacacg
    21721 aaacccgcca caatgaaacg cgccaaaaaa taccctttct taacgttgca gcggcaacgt
    21781 ttccatctga actttgaaaa cgcttcttcc gccgccaacc ttccaagcga acacgatttt
    21841 taccgctggg cgtggtctgc cctgaaaagc ggataccgcc gcgcagacat cagcctgatt
    21901 cttctggacg aagaagcagc ccgcgcctac aaccgcgact accggggcaa agactacgcc
    21961 accaatgtct tgagtttcgc gctcaacgaa ggcgaaaccc tgccctgcca agtttcggaa
    22021 aaactgtacg gcgatttgat tatctgcccg caagtcgttt tgaaagaagc cgccgaacaa
    22081 ggcaaaacgc ccgaacggca ttttgcccac ctgaccatac acggtgtttt gcacctgatg
    22141 ggctacgacc acatcgaaga cgatgaagcc gaaataatgg aagccgaaga aatccgcctg
    22201 atgcgggcgg caggctatcc caacccctac cgagaggacg gacattaaaa tggacggcgc
    22261 acaaccgaaa acaaattttt ttgaacgcct gattgcccga ctcgcccgcg aacccgattc
    22321 cgccgaagac gtattaaacc tgcttcggca ggcgcacgag caggaagtat ttgatgcgga
    22381 tacgctttta aggttggaaa aagtcctcga tttttccgat ttggaagtgc gcgacgcgat
    22441 gattacgcgc agccgtatga acgttttaaa agaaaacgac agcatcgagc gcatcaccgc
    22501 ctacgttatc gataccgccc attcgcgctt ccccgtcatc ggcgaagaca aagacgaagt
    22561 tttgggcatt ttgcacgcca aagacctgct caaatatatg tttaaccccg agcagttcca
    22621 cctcaaatcc attctccgcc ccgccgtctt cgtccccgaa ggcaaatcgc tgaccgccct
    22681 tttaaaagag ttccgcgaac agcgcaacca tatggcgatt gtcatcgacg aatacggcgg
    22741 cacatccggc ttggtcacct ttgaagacat catcgagcaa atcgtcggcg aaatcgaaga
    22801 cgagtttgac gaagacgata gcgccgacaa tatccatgcc gtttcttccg aacgctggcg
    22861 catccatgca gctaccgaaa tcgaagacat caacaccttc ttcggcacgg aatacagcag
    22921 cgaagaagcc gataccatcg gcggcttggt cattcaagag ttgggacacc tgcccgtacg
    22981 cggcgaaaaa gtccttatcg gcggtttgca gttcaccgtc gcccgcgccg acaaccgccg
    23041 cctgcacacg ctgatggcga cccgcgtgaa gtaagcactg ccgtttctgc acagtttagg
    23101 atgacggtac gggcgttttc tgtttcaatc cgccccatcc gccaaacata aaaatgccgt
    23161 ctgaaaccgt tgcaagtttc agacggcatt tcgcttatcc gcttattttt tgctcaaagt
    23221 cagccccgtc caaccgaata acagggtcaa agccaaactc aaatagcaga aaaaggcata
    23281 cggcagatat tcccaaaccg gcacgcccag cgcgtggctg atgaacacgc cgcatacgct
    23341 ccacggtacg agcgggttaa tcaccgtccc cgcatcttcc agcgtccgcg acagattgcg
    23401 ggaatgtagt ccgagcctgt cgtaaacggg tttaaacgtt tctccagaaa gcaggatgct
    23461 caaatattgt tcgccaatca ggaaattcac tccaaccgaa gtcatggcaa cgctgaacgt
    23521 cgcgcgtccg gcattcgtca agaaggtacg gatggcctcc aacagggaag gaatcacacc
    23581 gagcgcaaac aacagtccgc ccaaactcat cccgagaatc acgatggttt gcgtgaaaaa
    23641 catactttcc aaaccgccgc gcgaaatcag tttgacaaca tctttaaacg cttcgccttc
    23701 gagtttgtag ccgccgtaaa accacgcacc gagctgacgc agatcgggcg tgctgtgcag
    23761 atacgttaca gcaacggcaa ccatgacggt aaagagcatg gcgacgacgg cgttgatgcg
    23821 catcaatgcc aaaacgacca acagcgcaaa cggaatcagc gaatagccgt gcaccaatcc
    23881 cgtggcttca agctggctgc ggaaggattc gacgctgttc aaatcctgcg cggcgacgct
    23941 gggaagaagc cacagcatca gtgccgcact gatgagccac gcgggaatgg tggtgtacat
    24001 catatttttg atgtgttcaa acaggtcgat accgacaatg gacgcggaaa tgcccgtcgt
    24061 atcggaaagc ggggacattt tgtcgccaaa aaatgcgccc gaaacaatcg cgcccgccgt
    24121 catcgccata tcggcctgaa acgccgccgc catacccata aaggcaacgc cgacagtggc
    24181 gcaggtggtc aggctgctgc cgatggacac gccgatgacg gaacacagcg cgaaggcgga
    24241 aaaataaaaa taagtcgggg aaatcagtcc gaaaccgtaa tacatcagcg tcggaatcgc
    24301 gccgctcatc atcagcgcgc tgaccatcag cccgatgaag aaaaacaggt aaatcgcgcc
    24361 cataccctga ttcaacgcgc ctatcatgcc ctgctgcata tcgttgtatt tcaaaccgcg
    24421 cgccaagccg tacaaaatca gcacgacgat ggcggcaata atggacatat gcggcagcca
    24481 ctccaatgaa atgatggtat agcccatcgc ggcaatcaga gcgacgacga cggcaagtgc
    24541 ctcaccgcgc ggcatatcga gtaaggattt gaaagcgaac atgattcttt gcccgatttg
    24601 atataaagtt aacggattct aacacaccat accgaatccg cccgacgttt tcagacggca
    24661 ttttagcgtt ttacaaaagc cggacagaaa caaaacggca attttctcaa tttaaattaa
    24721 tttttttgaa atataatttt ttattttttt gtttgttgac atataagact tttatggatt
    24781 gaagtattaa aacctcggca cacacgccgc aaacaccggt acgcaaaaat gccgtctgaa
    24841 agatgttcag acgacatttc tcaagaaaaa gattatttgt ccgccctata tccgtacacc
    24901 gcaaaaaaca cgatatacag ataacagatg acggaaacga caaacgaaga catcagggtg
    24961 taagtatctg ccacccagcc ctgcacgaca ggaacgaccg caccgccgac aatcgcggta
    25021 cacagtacac cggaagccgc gttggtaaat tttcccaatc ctttggttgc caaagagaaa
    25081 atcgtcggga acataatcga gttgaaaaaa ccgatggcaa gcagcgacca catcgccaca
    25141 tccgcattgc cgctacccgt cgccatcgcg acggcaagca gtacgaccgc agccgatgcg
    25201 ttaaacgcca aataacggtt gggcgcgaat ttcgccatca ccgccgaacc gaggaaacgt
    25261 ccgaccatcg cgccgcccca atagaacgac aggtaatgcg cggcagaagc atgatccagc
    25321 cctttcagat aacccaatac gttgaccatc aacgaaccga tagacacctc cgcgccgaca
    25381 tagcagaaaa tgcctgccgt accgaacacg agatgtttgt attgccatac gccggttttg
    25441 ccgtcgtggt tgtgcgcgct ttcctcggcg gcaattttgc gcgcgtcggg cagccggatc
    25501 attttcacga aaacggcaag gataatcagc agccccgcca gtcccaaata gggaatctgt
    25561 acggaagaaa tctgttccgc cttgctgacg gtttgggttg cgtccgccaa aatcaggaac
    25621 gcgccgattt gcggcgcaat ggtcgtaccc aaagcgttaa acgcctgaac cagcgtcagt
    25681 gttgccgatt ccttgccggg tttcgccagc agggtaacat aaggattacc ggcgacctga
    25741 agcagcgtta cgccggaagc caaaataaac aacgcgccca aaaataccgc gtaagaatgg
    25801 ctgcccgcag caggataaaa cagcaggcat ccgaccgccg tcagcagaaa gccgccgata
    25861 acgccgtttt tgtagccgac tttgccgaca aaagccccca tcgggatgga catcaccgca
    25921 taggcagtaa agaaacagaa ttggatcagc atcgcctgaa cgtaagacag gtcgaaaatt
    25981 tctttcaaat gagggataag gatgtcgttc atgcaggtaa taaaacccat catgaagaac
    26041 agcgtggtca agacgaccaa tggggacgta tgattgtttt gtgattgcgc agacatgatt
    26101 tgcctttcgt ggtgtcggaa tattacccgt ttcaagaaaa tagaaattga catttcttaa
    26161 cggcaatgca aatcatactg atatttgaac attttgcaaa ctttatcgga tgcaggcaat
    26221 gcgccgatct gaaattcaag cagcagaaat taatatttta cagttgcttg catagatttt
    26281 aatagatttt aaaaaattcc ggcggacttg caaaaaccgt tcccaataaa atgaaaatcc
    26341 gccgccgtat ccggaaaatt cggaaatgcc gcatcgtggc atcaaaaccg gagcggaaag
    26401 aatgtcgtat gcccgacaaa aaaggggcat acggctgtgc cgtatttttt gcaccgtcct
    26461 ccgcccttcg gcgggggcgt gaaaatatag tggattaaca aaaatcagga caaggcgacg
//
LOCUS       DAWFJR010000020        26503 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWFJR010000020 DAWFJR010000000
VERSION     DAWFJR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26503
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.020"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            832..2280
                     /gene="gnd"
                     /locus_tag="WGR04_000760"
     CDS             832..2280
                     /gene="gnd"
                     /locus_tag="WGR04_000760"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ0794351.1"
                     /translation="MNGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTTAKVDEFLNG
                     AAKGTGIIGAYSLQDLVDKLEKPRKIMMMVRAGSVVDDFIEQLLPLLEKGDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGMGVSGGEEGARHGPSIMPGGDERAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2343..3614
                     /gene="waaA"
                     /locus_tag="WGR04_000761"
     CDS             2343..3614
                     /gene="waaA"
                     /locus_tag="WGR04_000761"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ0794352.1"
                     /translation="MFQWLYDALWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMRECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDIIPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKTLSSEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGYRGR"
     gene            3655..4104
                     /locus_tag="WGR04_000762"
     CDS             3655..4104
                     /locus_tag="WGR04_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ0794353.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSREARRE
                     VEKAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            4137..5111
                     /locus_tag="WGR04_000763"
     CDS             4137..5111
                     /locus_tag="WGR04_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0794354.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNPGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPEGDEKEDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFVFLKYGLA
                     FVLSFIGVKMLIMHWVHIPISVSLSVVFGALGASVLTSLVYTKKQSDK"
     gene            5180..5255
                     /locus_tag="WGR04_000764"
     tRNA            5180..5255
                     /locus_tag="WGR04_000764"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5213..5215,aa:Lys,seq:ttt)
     gene            5474..6727
                     /gene="murA"
                     /locus_tag="WGR04_000765"
     CDS             5474..6727
                     /gene="murA"
                     /locus_tag="WGR04_000765"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ0794355.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMVVEGS
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVEQLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            6808..7986
                     /locus_tag="WGR04_000766"
     CDS             6808..7986
                     /locus_tag="WGR04_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ0794356.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASIASV
                     KYCLDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALADLLKAADTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGE
                     "
     gene            complement(8054..8302)
                     /locus_tag="WGR04_000767"
     CDS             complement(8054..8302)
                     /locus_tag="WGR04_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0794357.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWNDKAQ"
     gene            complement(8393..9310)
                     /gene="ftsX"
                     /locus_tag="WGR04_000768"
     CDS             complement(8393..9310)
                     /gene="ftsX"
                     /locus_tag="WGR04_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ0794358.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITIYMETAAAQSDSDTVRSLLARDKRLDNIRFIGKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPDQMQAIYRDITKLPMVESANMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPGEIA
                     LVFGFVIALGIFGAWLATTQHLLGFKAKK"
     gene            complement(9307..9957)
                     /gene="ftsE"
                     /locus_tag="WGR04_000769"
     CDS             complement(9307..9957)
                     /gene="ftsE"
                     /locus_tag="WGR04_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ0794359.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYQPRKAEERARIAIEKVGLKGRELDDPITLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHESGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(10151..10633)
                     /locus_tag="WGR04_000770"
     CDS             complement(10151..10633)
                     /locus_tag="WGR04_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0794360.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDGAAQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKSYGNNIGVLPFTVVEAPKCGYRQTITGEANEKSLTEAVKLAHSKCR
                     "
     gene            10757..11110
                     /gene="arsC"
                     /locus_tag="WGR04_000771"
     CDS             10757..11110
                     /gene="arsC"
                     /locus_tag="WGR04_000771"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ0794361.1"
                     /translation="MSEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPNLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            11107..11763
                     /locus_tag="WGR04_000772"
     CDS             11107..11763
                     /locus_tag="WGR04_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ0794362.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPFLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKHPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGRDFFNWLFH
                     "
     gene            11956..13350
                     /locus_tag="WGR04_000773"
     CDS             11956..13350
                     /locus_tag="WGR04_000773"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ0794363.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGKFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNADNVVYQTRRFDRYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPTGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNDKLAELVKPRLA
                     LRGLHETGKPALEDVLALVKDRAQDLNTLADECLYFYVKQMPAEADVQKHWDGEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            13371..13766
                     /locus_tag="WGR04_000774"
     CDS             13371..13766
                     /locus_tag="WGR04_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794364.1"
                     /translation="MPSDVGMRLQTAFKRKLNMKKILYFLMFVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQTAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            13770..14261
                     /locus_tag="WGR04_000775"
     CDS             13770..14261
                     /locus_tag="WGR04_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0794365.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDFGTLLGSAAVKRHDMKSFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(14385..16979)
                     /gene="mutS"
                     /locus_tag="WGR04_000776"
     CDS             complement(14385..16979)
                     /gene="mutS"
                     /locus_tag="WGR04_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ0794366.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSANGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17423..18427
                     /gene="gap"
                     /locus_tag="WGR04_000777"
     CDS             17423..18427
                     /gene="gap"
                     /locus_tag="WGR04_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0794367.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18497..18616)
                     /locus_tag="WGR04_000778"
                     /pseudo
     CDS             complement(18497..18616)
                     /locus_tag="WGR04_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18685..19365)
                     /gene="tsaA"
                     /locus_tag="WGR04_000779"
     CDS             complement(18685..19365)
                     /gene="tsaA"
                     /locus_tag="WGR04_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ0794368.1"
                     /translation="MTYTIVPIATARSPYKQKFGIARQPGLVSAAEVCIELNPEFTAD
                     SVRGLEHFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQDIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            19480..20115
                     /locus_tag="WGR04_000780"
     CDS             19480..20115
                     /locus_tag="WGR04_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ0794369.1"
                     /translation="MIVSIDVDAQKTFTPLCPKELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHAAAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPCAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ"
     gene            20152..21120
                     /gene="waaC"
                     /locus_tag="WGR04_000781"
     CDS             20152..21120
                     /gene="waaC"
                     /locus_tag="WGR04_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ0794370.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFRAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKMAKSPICGLDKNSAREGWAALAYVKTYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            21347..22096
                     /locus_tag="WGR04_000782"
     CDS             21347..22096
                     /locus_tag="WGR04_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ0794371.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDAKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLAPADLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            22107..23042
                     /locus_tag="WGR04_000783"
     CDS             22107..23042
                     /locus_tag="WGR04_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ0794372.1"
                     /translation="MSVLIIAEHDNKQLNPATLHAVTAAAKLGKVDLLVAGNGASSVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQANAEKLVLTFRATAFDAAAVQGGN
                     AEVINIETTPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEALKN"
     gene            23105..23716
                     /locus_tag="WGR04_000784"
     CDS             23105..23716
                     /locus_tag="WGR04_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ0794373.1"
                     /translation="MITTYKTITSPTQAGFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVSGLIHAYKEATAQALAIAEVIEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     ILEQDLQLDCRLTVSLSLANYEVCVAAWKNTRQIEVNTEKPFE"
     gene            complement(23768..24085)
                     /locus_tag="WGR04_000785"
     CDS             complement(23768..24085)
                     /locus_tag="WGR04_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ0794374.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKYVVTLIRDMDNFYT
                     HEYQACHQAIEDGVELDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            24243..25514
                     /gene="purD"
                     /locus_tag="WGR04_000786"
     CDS             24243..25514
                     /gene="purD"
                     /locus_tag="WGR04_000786"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ0794375.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFSRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGRLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            25548..26150
                     /locus_tag="WGR04_000787"
     CDS             25548..26150
                     /locus_tag="WGR04_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ0794376.1"
                     /translation="MTDVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            complement(26221..>26503)
                     /locus_tag="WGR04_000788"
     CDS             complement(26221..>26503)
                     /locus_tag="WGR04_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0794377.1"
                     /translation="PDSIVYTDSLSSCDKLDVSGFIHYRINHSKEFADRQNHINGIEN
                     FWNQAKRVLRKYNGIDRKSFPLFLKECEFRFNFGTLSRQLKILQDWCGI"
BASE COUNT         6359 a         6104 c         7455 g         6585 t
ORIGIN      
        1 atcccgtcat tcccacgaaa gtgggaatct aggacgcgga atctcaagaa accgtttacc
       61 cgataagttt ccgtgccgat ggttctagat tcccgcctgc gcgggaatga cggcggagcg
      121 gtttctgttg ctcccgataa attcctaaaa ctcaaaattt catcattccc actttcgtgg
      181 gaatgacgga atgtaggttc gtgggaatga cgcggtacag gtttccgtta cgtatcccca
      241 tccgaaagtt tatttataca caagcaatcc aaagttatct gactgaaata ttgtgagatt
      301 tacccgcaaa tctcattttt tccaccactt atacacaaat tcggatgcgc actctgccga
      361 tatgcagaaa taagacattc gtgccgacac ctgaccattt ccacaagttc caaacacact
      421 ttcctctcaa acctccacag tattccggtt cttaaagaca tcggcagcca ttcggtaaag
      481 aaaagtagga tgccatttgc acaacaagca aactgtccac aaaaactatt cttcatgaaa
      541 aacaatccac aggtatgcac aaaagggaat ccgccttacc gacaccctaa aaaaaagaaa
      601 tcctgatgat ttttaaaata aaaaaaagtt acccacagaa aatttctcct atcaatataa
      661 tcatcgtttt attatttaaa ttttatattt tatttataac ccttcagcaa aacggtacag
      721 caaagcgtaa aggcatcttt tcaaaaaatt aaaaagttaa ggattgttaa acaagtgtaa
      781 atcgggtaga gttgcggtcg ataaattaaa tctataacgg agcttcctgc aatgaacggc
      841 gatattggtg taatcggttt ggcggttatg gggcaaaacc tgattttgaa tatgaacgat
      901 tgcggattta aggtggttgc ctacaaccgc acgactgcca aggtggacga atttttaaac
      961 ggcgcggcaa aagggacggg cattatcggc gcgtattccc tgcaagactt ggtcgataag
     1021 ctggaaaaac cgagaaaaat catgatgatg gttcgcgcag gttcggtggt tgacgacttt
     1081 atcgaacaac tgcttccgtt gttggaaaaa ggcgacatca ttatcgacgg tggcaatgcc
     1141 aattaccccg acacgacacg gcggacacat taccttgccg aaaaagggat tttgtttgtc
     1201 ggcatggggg tctccggcgg agaggaaggt gcgcggcacg gcccgtctat catgccgggc
     1261 ggagatgagc gcgcatggga agctgttaaa ccgattttcc aggcgattgc tgcgaaaacc
     1321 cctcaaggtg agccgtgttg cgattgggtc ggcagggatg gtgcgggaca tttcgtcaaa
     1381 atggtgcata acggcatcga atacggcgat atgcagttga tttgcgaagc gtaccagttc
     1441 atgaaagacg gtttgggact gtcctacgac gaaatgcacc gcgtgtttgc cgagtggaac
     1501 aaaaccgagc tggattctta tctgattgaa atcacagcgg caattttggg ctataaggac
     1561 gaaggcggcg aaccgctggt cgagaaaatc ctcgatacgg cagggcaaaa aggcacgggc
     1621 aaatggacgg gcatcaatgc tttggatttg ggtattccgt tgacgctgat ttccgaagcg
     1681 gtattcgccc gctgcgtttc gtcgttcaaa gaacagcgcg tgcagaccgg caagctgttc
     1741 gcccgaaccg ttacgcccgt cgaaggcggc aaacaagaat gggttgaggc gttgagacag
     1801 gccctgcttg catccaaaat catttcctac gcacaaggct ttatgctgat ccgcgaagcg
     1861 ggcgaaagct acggctggga tttggactac ggcaacactg cgctgctgtg gcgcgagggg
     1921 tgcatcattc gcagcgcatt cttaagcaat atccgcgacg cgtatgaaaa caatcccgat
     1981 ttggtgttct tgggtgcgga tccgtatttc aaaaatattt tggaaaactg cctgccggcg
     2041 tggcgcaagg tggttgctaa ggcggtcgaa tgcggcatcc ctatgccctg catggcttcg
     2101 gcgattacgt tcttggacgg ctacacgacc gaacgtctgc ctgccaacct cttgcaggca
     2161 cagcgcgact acttcggcgc gcacacttac gaacgcaccg acaaaccgcg cggcgagttt
     2221 ttccatacca actggacggg caagggcgga gataccgctt cgacaaccta cgatatttga
     2281 ttccgttgcc gaaatgccgt ccgaaccgtt cggacggcat ttgttttgca ggaaatagaa
     2341 agatgttcca atggctttat gatgcattgt ggctgcttgc gccgatatgg atacggcgtt
     2401 atttggacaa acgctccgga agtgcccctg catatcgggc gcatcgggac gagcgtttcg
     2461 gcaagccgta tccgaatccc gttaccggcg cggtttggat acacgccgtt tcggtcggag
     2521 aaacgcgtgc cgcccagccc ttgatacgcg agttgcggca gcgttttccc gatgcgccgc
     2581 tgctgatgac gcagatgacc ccgacggggc gggaaaccgc gcaagttctg tttcccgatg
     2641 cgcaatgccg ctatcttccg tatgacaaaa aaacgtgggt acggcagttt ttgcgcgaac
     2701 accgcccgat gttcggcatt ttgatggaaa ccgaaatctg gcccaacctg atgagggaat
     2761 gccggcgcgc gggtgtgccg ctgtttttgg cgaatgcgcg gctgtcggaa aaatcgttga
     2821 acggttatct gaaagtccgc cgcctgatcc gtcctgccgc cgcttcgctg acggggtgtc
     2881 tggcgcagac agaggcggat gcggcgcggt tggcgaaatt gggcgcggca tccgtgcagg
     2941 tgtgcggcaa taccaaatac gacatcatac cgtcggaaca gatgaaaacg ctggcggggc
     3001 agtttgaaaa acgcatcgga ggccggccgg ttgccgtgtg cggcagcacg cgcgtttatc
     3061 ggggtgaaga cgaggcggaa aaactgctgg cggcgtggca acaatatcgc ggcgatgcgc
     3121 tgctggtcgt cgtgccgcgc catcccgagc attttcagac ggtatttgaa acggcaaaac
     3181 gcttcgggtt taaggttcag cggcgcagcg acggtttgcc ggtcgaacct gatacgcagg
     3241 tgtggatagg cgacagtatg ggcgagctgt atgcgtatta cctgtgcgcc gatgtcgctt
     3301 ttgtcggcgg cagtctggtc gattcgggtt gtcagaacat catcgaaccg ctttcctgcg
     3361 gcgttccgac gatattcggc ttttcaacct acaatttttc cgaagcctgc cgacacgcct
     3421 tggcatcggg tgcggcggtt caagtcgaat cggcggatgc gtggcgggaa gccgttgaaa
     3481 aaaccttatc gtccgagggg ggggggatgc agatgcaggc gcgcgtggac ggctttatcg
     3541 cacaacatcg cggagcgggc gcgagaatcg ccgaggcggt gcgggaagcg gtatgcggat
     3601 atcgggggcg atagtgatac aatccgtatc cgagttttcc gtttggagca tcgtatgagt
     3661 atttatgccg tcgcgcacat cgttcatctg tattgcgcta ttgcctttgt cggcggcgtg
     3721 ttttttgaag tgctggtttt gtccgtcctg catacggggc gggtgtcgcg cgaggcgcgg
     3781 cgcgaagtgg aaaaggcaat gtcttaccgc gccgtcaggg tgatgccgtt tgtggtcgga
     3841 ctgctgttcg ccagcggcat cgtgatggcg gcaaaccgct atctttctat attgggcgaa
     3901 ccgtttgcca cttccttcgg tacgatgctg acgctgaaaa tcctgttggc gttcagcgta
     3961 ttggcgcact tcgccatcgc cgtcgtcaaa atggcgcgtt ccacactgac ggtcggctgg
     4021 tcgaaataca tacacgccgt cgtctttacc catatgctgc tgattgtctt tttggcaaaa
     4081 gcgatgtttt atatcagctg gtaacacaac tacatttcaa atcaggagat tgaaccatga
     4141 cagaatatcc cggcatcggg tcgccgttgt tttacggcgt tttttttgcg gcagtgctgg
     4201 tcatgattgc cttggatatg ttttcgctga agaaaaacgg cagccataaa gtcggcgtca
     4261 aagaagcctt ggcatggagc ggcttgtggg ttgccgtatc ctgcctgttc gcgggctggc
     4321 tgtatttcga actcgccggc aatcccggct acggcgcggc agtcgcaaaa gaaaaagtat
     4381 tggaattctt taccggctac attttggaaa aatcgttggc agtcgacaat attttcgtgt
     4441 tcctgatgat attcggctac ttcaaagtcg caccgcagtt tcagcaccgc gtgctgctgt
     4501 acggcgtatt gggcgcattg gtattgcgca ctgtcatgat tttcgtcggc gcggcactgg
     4561 ttcggcagtt tgagtggatt ctgtatctgt ttggcgcgtt cctgctctat actggtatac
     4621 acatgatgaa gcccgaaggc gatgagaaag aggatttggc aaacagcagg ctgctgaatg
     4681 ccgtaaagaa agtcgttccg gtcggcacgg aatttcacgg cgagaaattt tttaccgtcg
     4741 aaaacggcaa aaaaatcgcc acgccgctgt ttttggtgct ggtcatgatt gaattgagcg
     4801 atgtcgtgtt tgccgtggac agcatccccg ccgtctttgc cgttaccacc gatccgttta
     4861 tcgtgctgac ttccaatatt ttcgctattt tgggcttgcg ggcgatgtat ttcctgctgg
     4921 cggatgtggc ggaacgcttc gtattcctga aatatggatt ggcattcgtg ttgagtttta
     4981 tcggtgtgaa gatgctgatt atgcattggg tgcatatccc gatttccgtt tcgctgtcgg
     5041 tcgtgttcgg cgcattgggt gcatcggtgt tgacttcatt ggtttatacg aaaaaacagt
     5101 ccgataaata aaatgccgta tgagcgccga caaaatggtt gacagcatca ggcttgatgt
     5161 atagaatttc gagcttatcg ggtcgttagc tcagtcggta gagcagcgga cttttaatcc
     5221 gttggtcgag cgttcgaatc gctcacgacc caccagataa aacaaaaaag ctgagttttc
     5281 aaaactcggc tttttttgtc ttccgacaat ccttgttcgg aaagcgcggc atttcgtcaa
     5341 attctttggt gcggcttccg tatttttccc gaaaatccct tataattccg cctctgcaac
     5401 cttcatcggg tgatgcttta tgcgttgtcc cgagtattga aatattacaa gtacccacca
     5461 ggaaagaaca aacgtggaca aactgaaaat ctccgcaaac ggcccgctca acggggaaat
     5521 aacggtctca ggcgcgaaaa acgcggcatt gccgctgatg tgcgcgggtt tgctgacatc
     5581 gggtacgttg cgcctgaaaa acgtccctat gctggcagat gtgaaaacca cgcaaaagct
     5641 gcttcagggg atgggcgcgc gcgtcctgac cgacaatatc agcgaatttg aaatcaacgg
     5701 cggtacggta aacaatacct gcgcccctta cgaattggta cggacgatgc gggcctcgat
     5761 tttggtactg gggcctactt tggcacgttt cggcgaggcg caagtcagcc tgccgggcgg
     5821 ctgcgccatc ggttcgcgcc ccgtcgatca gcatttgaaa ggcttggaag cgatgggggc
     5881 ggaaatcgtc atcgaacacg gttacgtcaa ggctaagggt aaactcaaag gtacgcgcgt
     5941 ggcgatggat gtcgttaccg tcggcggcac ggaaaacctg ctgatggcgg cgacgctggc
     6001 ggaaggtacg acggttttgg aaaactgcgc cattgagcct gaagtggtcg atttggcgga
     6061 atgcctggtc aaaatgggcg cgaaaatcag cggcatcggt acgtccacga tggttgtgga
     6121 aggctcggac gagctgcacg gctgcgaaca cagcgtcgtc cccgaccgga ttgaagcagg
     6181 gacgttcctg tgcgcggtgg cgataaccgg cggcagggtg gttttgcgga atgccgcgcc
     6241 gaaaacgatg gaagtggtgt tggacaaact ggttgaggca ggtgcggtga ttgaggcggg
     6301 cgacgattgg attgccatcg atatgcggca gcgtccgaag gcggtggaca tccgcacggt
     6361 cgtccacccc ggcttcccca cggatatgca ggcgcagttt atggcattga atgccgtggc
     6421 agagggaagc tgccgcgtgg tggaaacgat ttttgaaaac cgctttatgc acgtccccga
     6481 gttgaaccgg atgggggcga acattacaac cgagggcaat acggcatttg tgcagggtgt
     6541 ggaacagctt tccggcgcgg tcgtcaaggc gacggatttg cgtgcgtccg ccagcctcgt
     6601 tatcgccggt ttggcggcgc gcggcgaaac cgtggtcgaa cagatttacc acttggatcg
     6661 cggttatgaa aatattgaaa aaaaactcgg cagcgtcggc gcgaaaatcg agcgcgtatc
     6721 cggctgatcg ggttgcgatg ccgtctgaag ccttcagacg gcatcaattg tttccgtatc
     6781 gcaaacaccg attaaaaagg actgattatg gcatttttaa aactgaccga acaaaacgtg
     6841 cagggcaaaa ccgtcctcat ccgcgccgat atgaacgtgc cgttcaaaga cggcaaaatc
     6901 agcgacgaca cccgtatccg cgcctcgatt gcgtccgtca aatactgctt ggacaacggc
     6961 gcgtccgtta tcgtgatgac ccacttgggc cgcccgactg aaggcgagtt ccaccccgaa
     7021 gacgacgttg cgcccgttgc cgcgcatttg ggcggtctgt tgggcaaaga cgtaaaagta
     7081 ttgaacgact ggcgcgaaaa caaaccggct ttgaacgcgg gcgatgtcgt gatgctgcaa
     7141 aacgtgcgta tcaacaaagg cgagaagaaa aacgatttgg aactgggcaa agcctacgct
     7201 tctttgtgcg acgtgttcgt caatgacgcg ttcggcaccg cgcaccgcgc ccaagcctcg
     7261 accgaagccg tcgcccaagc cgctcccgtt gcctgcgccg gcgtattgat ggcgggcgaa
     7321 ctcgacgctt tgggcaaagc cttgaaacag cccgcgcgcc cgatggttgc catcgttgcc
     7381 ggcagcaaag tgtccaccaa gctgaccatc ctcgaatcgc tggcggacaa agtcgaccaa
     7441 ctcatcgtcg gcggcggtat cgccaacacc ttcctgttgg cggaaggtaa agccatcggc
     7501 aaatctttgg cggaacacga tttggtggaa gaatccaaaa aaattatggc gaaaatggcg
     7561 gcaaaaggcg gttctgtgcc gctgccgacc gatgtcgtcg ttgccaaagc ctttgccgcc
     7621 gatgccgaag cggtggtaaa agacattgcc gacgttgccg aagacgagat gattttggac
     7681 atcggcccga aatctgccgc cgcgcttgcc gatttgctta aagccgccga cacggtcgtt
     7741 tggaacggac cggtcggcgt gttcgagttt gaccagttcg caggcggtac gaaagccctt
     7801 gccgaagcca ttgcccaaag caaagcgttc tccattgctg gcggcggcga cacgctggcg
     7861 gcgattgcca aattcggcgt taccgaccaa atcggctaca tctccaccgg aggcggcgcg
     7921 ttccttgagt tcttggaagg taaagagttg cctgccgtag ccgctttgga aaaacgcggc
     7981 gagtaaacgg tttgatataa aaaaatgccg tctgaaacgt tgtgctgttt cagacggcat
     8041 ttttgtgtgg cggttattgt gctttgtcat tccattctgc cggtgtggcg gctaccgaaa
     8101 tggaaagtgc gtgcagttcg ttgctttcca gttgggcttt aagcccgtct ttaatcaggc
     8161 ggtggcgcgc gaggcgtgcc ttgccttcaa attctgatga aacgatgacg gcgaaaaaat
     8221 gatgtccgtc gccttctact tcgatgtgtt cgcattttgc cacgccttca atcatggctt
     8281 ttacttgttc cgaggtcagc atggtaggtt ccttgttgga aaaatgcgcc attatactgg
     8341 caaattgaaa tgccgtctga aaacgggttc ggacggcatt tttgacggtg ttttattttt
     8401 tggctttgaa gccgagcagg tgctgggtgg tggcaagcca cgcgccgaat atccccagtg
     8461 cgatgacgaa gccgaacacc agtgcgattt ctcccgggta gaagaaacgc cagccgatat
     8521 taagtccgta gggtttgaag atggcatcga ccaaggggcg cacggcagag agcagccaac
     8581 cgcaaagccc caagctgacg gcggcggaaa ggatgctctg ccacatggct tgataaagga
     8641 atgggcggcg gataaacgac gcgggcgcgc ccaagagttt ggtgatttcg atttcttctt
     8701 ttcggctgag gatttgcagt cggatggtgt tgtgcgcgac aaggacgaac gccatcccca
     8761 gcgtcaggga aagaaaccat aagattttac ggatgaattc gttgatttgg tacagtgtct
     8821 gtacccattc cgtatccatg ttggcggatt cgaccatagg cagtttggta atgtctcggt
     8881 agattgcctg catttggtcg ggcgtggttg ccgggtcggg ggtaacgata aagacatccg
     8941 gcagggggtt gccgtcaagc atggaaatca gattttggtc gagattggac tgtaattctt
     9001 ccagaccgtc ttctttgccg atgaagcgga tgttgtcgag ccgtttgtcg cgcgccagca
     9061 ggctgcgcac ggtatcgctg tcgctttgtg cggcggcggt ttccatatag attgtgattt
     9121 gcggcgactc gttgagtttg cctaaaacgc tttgtccgct ttggatgccc aaatacataa
     9181 acagcggcag ggtcatggcg acggcgagca ttatgagggt aagcagtgtg ccgaaaggtt
     9241 ggcgcagaag ctgtttgagc gcggtgcgcg cggattcgac gtgcagcgag aggtagtgga
     9301 tgatgctcat gcgagtcgtc ctttcgagag gcgcaggatg cggtgtccgt agtccgccat
     9361 cagggtttcg tcatgtgcgg cgacgatgac ggttgttccc gattcgtgga aggttttgaa
     9421 caattccata atatcgagcg cgtaggcgcg gtcgaggttg gcggagggtt cgtcggcaat
     9481 cagcaggccg ggctggtgaa cgacggcgcg ggcaatgcac aggcgttgtt gttcaccgcc
     9541 ggagagggtt ataggatcgt ccaattctcg ccctttcaag ccgacttttt caatagcgat
     9601 gcgggcgcgt tcttcggctt tgcgcggctg ataaccgata atccgcagtg ggaggatgac
     9661 gttttgcagg acgttgcggt cgtagaggat tttgtggtct tggaacacga tgccgatatg
     9721 ttggcgcata aagccgattt ggttgtcgga caatgtgccg agatcctgcc cgttaaacag
     9781 gattttgccc ctgctcggct tggtgatgcc cgaaatcagc ttgaggacgg tggatttgcc
     9841 cgaaccggag tgtcccgcga taaatatcat ttcgcctttg ttgatttgga agctgacgtt
     9901 tttcagggct tcaaaaccgc cgggataggt tttggaaact tgttcgaaac ggatcatagg
     9961 aggtcctgta aggaagggcg gcatatcgtt tcgggcggtt gcgtccgcag ggatatgccg
    10021 tctgaacaat aggtaagaca gtgccgatta taaagattcc gcctgttttt agatagtgga
    10081 taagtttggc ggatatgaag gtcgggcggg aaaaatgccg tctgaagcgg cttcagacgg
    10141 catccggcgt ttaacggcat tttgaatggg cgagtttgac ggcttcggtc aggctttttt
    10201 cgtttgcctc cccggtaatg gtctgcctgt atccgcattt cggtgcttcg acgacggtaa
    10261 agggcagtac gccaatattg tttccgtagg atttcataaa gtttcggctg tttgccccgg
    10321 tgtaacgcca aatcgggtag gaaacaggag tttgtttgag gaagttgccg atattgtcgg
    10381 atgtgtcgag cgcgatgccg accatatcga cgctgccttt tttctgcgct ttgtaccatt
    10441 tggacatggc aggcatctct tttcggcacg ggccgcacca agtcgcccaa aggttgacga
    10501 tgcgtacggg ggctttgagc gattgcaggc tttgcgcggc accgtctttc catccggcca
    10561 gttcgtctgc cgaggcggta tgtgcggcaa atgtggcggc aagggtgatt gcggcaagat
    10621 tcaagtattt catcgttttg tcctttgtat gtcgattcgg gtattgtaaa ccgtttggta
    10681 cggttgtagg cgggatattg agtaaaaccc tgtgtaagcc gataataagg ggattgtcga
    10741 gaagtaagga aatgtcatgt ctgaaatcaa aattttccac aacccccgtt gcagcaaatc
    10801 gcgtgccgcc ttgtccctat tggaagaacg cggcattgct gccgaagtgg tcaaatattt
    10861 ggatacgccg cccgacttgt ccgaattgaa ggatattttc aacaaattgg gtttggcatc
    10921 ggcgcgcggg atgatgcgcg tgaaagatga tttgtataag gaattgggtt tggacaaccc
    10981 caatttggac aatgacgcgc tgctgcgtgc catcgccgat catcccgccc tgttggagcg
    11041 tccgattgtt ttggcaaacg gtaaggcggc tgtcggcagg cctctggaaa atatcgaagc
    11101 ggtattgtga acaaacggct gttttgcagc cgcaacggct tgcgctatta cctgttgggc
    11161 ggtttctgtt tgagtgtttt tccgtttttg ctggtgtttg cctcatccgt ttgggcggtt
    11221 tatcggacgg gcgggcaggt tttgccgccg tatgtccggg cggatgccgc gctggtgttg
    11281 ggtgccgccg cgtgggacaa acacccttct ccggtttttc gggaacgcat caaccacgcc
    11341 atcgcgcttt atcagagccg cagggtaggc aaaatcgtgt ttacgggagg gcgaaccaaa
    11401 aaaggctata tgaccgaagc cgaggtcggg cggcggtacg cgctcaaaca gggggttccg
    11461 gcgcgcaaca tcctgtttga aaacacttcg cgcaacactt atgaaaacct gaacaacatc
    11521 cgtccggttt tgcgtgccaa cggtattgcc agtgtggtga ttgtcagcga tccctaccat
    11581 ttggcgcggg cggcggaaat ggcggaggat ttgggtgtcc gcgtttatat gtctgccacg
    11641 ccaaccacgc gctttgatgc gggaaataag aaaaaaatct ttatgctgca agagggatat
    11701 gcgttgtccc tctaccgttt ggaaaaatgg gggcgggatt ttttcaattg gttgtttcat
    11761 tgaacctttt tttcggacgc aatcccgaag gaacccggtc ggcggcgcgt gatacactgt
    11821 tccgcgtgcc gaagtattga aaacaaggaa aatgccgtct gaaacttatc cgcaggcagg
    11881 gttcggacgg catttttaat gcccaagcac ggtaaaacca gtaaaataaa cgctttcaaa
    11941 gatttcggag ccattatgac cgtcaaaacc cgtttcgccc ccagccctac cggctacctg
    12001 cacatcggcg gcgtacgcac cgccttgttt tcttgggcgt ttgcccgcca tcataaaggc
    12061 aagttcttat tgcgtatcga agacaccgac ttggcgcgtt ccaccgccga atccgtcaac
    12121 atcatcctcg acggtatgaa atgggtcgga ctcgattacg acaacgccga caacgtggtg
    12181 taccaaaccc gccgtttcga ccgctataaa gaagtcatcg ccgaactctt ggcaaaaggc
    12241 gatgcctact actgctattg cagcaaagaa gaattggaag cgatgcgcga gaaagccgaa
    12301 aaagaaggca cggcgactta cgaccgccgc tggcggccgg aagcgggcaa aaccctgccc
    12361 gaaattccca ccggcgtgca acccgtcgtc cgcttcaaaa cgcctttgga cggcgtcacc
    12421 aaatggacgg acttggttaa aggcgaaatc tctattccca acgaagcgct tgacgacctg
    12481 attatcgccc gcgccgacgg cacaccgacc tacaacttct gcgtcgtggt ggacgactac
    12541 gacatgggcg ttacccacgt tatccggggt gacgaccatg taaacaatac cccgaaacaa
    12601 atcaatatct taaaagctat tggcgcaaac ctgcccgaat acgggcacct gccgatgatt
    12661 ctcaacgaac aaggcaaaaa aatctccaaa cgcagcggcg ataccgttgc cattaccgat
    12721 ttcggcgcga tgggcatcct gcccgaagcg atgctcaact atctggcacg cttgggctgg
    12781 gcgcacggcg acgatgagtt cttcacgatg gaacaattta tcgaatggtt tgatttgaaa
    12841 gacgtttccc cgtccccaag ccgtatggat ttgaaaaaac tctattggat caacggcgaa
    12901 cacatcaaaa tcacacccaa cgacaagctc gccgaactcg tcaagccgcg cctcgcgctg
    12961 cgcggacttc acgaaacagg caaacctgct ttggaagacg tattggcatt ggtcaaagac
    13021 cgcgcccaag atctgaacac gcttgccgac gagtgcctct acttctacgt caaacaaatg
    13081 cccgccgaag cagacgtgca gaaacattgg gacggcgaag ccgccgcccg tatgctgcgc
    13141 ttcgccgaac gccttgaagg tttggaagac tggaacgccg aagccatcca cgatctgttc
    13201 aaacccttct gcgacgaaga aggcatcaaa atgggcaagc tcggtatgcc cctgcgcctc
    13261 gccgtctgcg gtacggcgaa aaccccgagc gtcgatgccg tattggcatt aatcggcaaa
    13321 gaagaagtgt tgaaacgcat ccgcgcttaa gttatccaca ggcgtgggcg atgccgtctg
    13381 atgtcggaat gcggcttcag acagcattca aacggaagtt gaacatgaaa aaaatattgt
    13441 attttctgat gtttgttttt tctacaagcg tatgggccgg ggatgcggaa gacaatctgc
    13501 tcagcatcca atccggctat cgtgccttac tgcaaaaaca aaacaatctg gacggaaaaa
    13561 tcatcgggat gcagtcggat ttggaagatg cgcgccggcg tttgcagacg gctcaagcag
    13621 acatcgcccg tttggaagcg gaaattcctg cagcaatggc gcaaaaagcc cggcaggctg
    13681 aagagctgag gcaaatcgga gtgcgtttgg accatgcttg gaatgcggtt tacggcgcag
    13741 ggggaacgaa ggcgtcgggg aattgaccga tgaattccct ctttgtggac aatactgttt
    13801 tcattacacg gctgaaagcc gggcatatcg gcaggttggt tcaggcgttg tttgaggagt
    13861 ggcacggatt tgaaccgtgg tcttctgtgg ataagattcg tgcctattac ggcagatgtt
    13921 tgaaagatga cgaactgccg ctggcttttg cggctgtgga tgatttcgga accctgttgg
    13981 gttcggctgc ggtcaagcgg catgatatga aaagtttccc acagtatgaa tattggttgg
    14041 gggatgtctt tgttttacct gaatatcgcg gaaaaggcat tggcaggagg ctggtcgccc
    14101 attgcatagg cgcagcgcgt tcgctgggga taaagttctt gtatctttat acgcctgatg
    14161 tgcaaatatt ttatgaatca ttcggctggg cggttgtcgg gcgacatttc cataacggtg
    14221 aatgggttac ggttatgcgt ttggatatgg ataaggttta agccgaatgg acaaatgccg
    14281 tctgagctgt tcggacggca ttttttatat ttggaaattt cctgtcagat aatgtttatc
    14341 cacagatttt tccatatggt ttgatgctgt tccgacaacg gaaattaaga tacggatttg
    14401 cacaaatctt tcagacggta cagttctgac aatgcttcgc gcggggtcag gctgtcggga
    14461 tcgagttttt ccaaggctgc tgccaatata ccttcaaaat gtttttcctc tgctttatcc
    14521 acaaagttgc ccacattcgg ttcatctcct ttttcagacg gcatggtact gaaaatatcc
    14581 agttggggac ggtttgcggc ggcttggttt tccagtccgt tcaaatgctt ttgggcggat
    14641 ttcaatgcgc gtacaggcag gccggcgagt ttggcaacgg cgatgccgta gcttttgccg
    14701 gcgggccccg gttggatttg gtgcagaaac acgatgtcct gtccctgttc gagcgcggaa
    14761 aggtgcatat tgacggcggc ggcgtgggct tcgggcaggt aggtcagctc gaaatagtgg
    14821 gtagcaaaca ggctgaagga tttgtttttt tgcagcaggt gttcggcaac ggcgtgcgcg
    14881 agggcgaggc cgtcgaaagt ggaagtacca cgtccgactt cgtccattaa aacaaggctt
    14941 tgttcggtgg cgtgatgcag gatgtaggcg gtttcgctca tttcgaccat gaaagtggag
    15001 cggttggagg cgaggtcgtc cgatgcgccg atgcgggtga agatttgatc gatgggcccg
    15061 attgtggcgg catcggcagg cacaaaacag ccggtgtgtg ccaataaaac aatcagcgcg
    15121 acttggcgca tgtaggtgga tttgccgccc atattggggc cggtgagcag catgaggcgg
    15181 tgtttgtggt caaggtcggt gtggttggcg gtgaagtggc gtacctgctg ttcgacaacg
    15241 ggatggcggc cgttttcgat gtggataacc ggatagtcgg caaactcggg gcggacgaag
    15301 ttccgctctt ttgccaaggc tgaaaatgtg gacaacacgt ccagcgcggc ggcggctttg
    15361 gcggcttttt gaagctgcgg caatgccgtc tgaaggtttt tcaatacgcc gtcaaagagt
    15421 tgtttttcta aggcgagggc ttgctcttga gcagtcagca ctttgtcttc aaaggctttc
    15481 aactccggcg tgatgaagcg ttcggcgttt ttgagggttt gccggcgttg gtagtcggca
    15541 ggtgcttgtt cggcttgggt tttggacaat tcaatgtaaa agccgtgaac gcggttgaac
    15601 tcgactttga gtgtggacaa accggtacgt tcgcgttcct tggcttccaa atccagcaaa
    15661 aattcgtcgc catggttttg aatgcggcgc aattcgtcca gttcgggatg aaaaccgtgg
    15721 ttgatgacat tgccgtcttt cagccagacg gaaggttcgg gcaaaatggc ttggcggagc
    15781 tgttcggctg tggataggtt ttccgggaaa acggctttga gggtttccaa caggctgctg
    15841 ccgtttgcgg acaagtcgat ttgtgccaac tcgaacagac tgtcgcgtaa agatgccaaa
    15901 tcgcgcgggc gggcgttgcc gacggcgatg cgggcagcaa tgcgttcaat gtccgcaatg
    15961 tttttcagac ggcattggag gggtttgtat tggctttcca gcgcggcaac ggcttcttgg
    16021 cgcgctcgga tgtgggcgcg gttgcgtaaa gggtggtgca gccagagagc caagaggcgg
    16081 ctgcccatat gggtagcgca aaggtcgagc gtggacatca gggtcggcga ttttttgccg
    16141 gagagggttt gcgtgatttc gagattgcgg cgcgtggcgg catccatacc gatatattgg
    16201 ctgtcggttt cgagcgacag gccgtccaaa tgttgcggca tcaggttttg cgtcaggcgg
    16261 atatagttca acagtgcacc tgccgcgcca atcgcaacgg cgtgttcttt gccgtccaaa
    16321 ccgaagccgc gcaaatcctg gcagccgaaa tattccgtca gcagtttttc ccccgcgtcg
    16381 gcggcaaact gccacgcgtt caggcgcgta acacccgatg ccgtctgaag ttgcggtgcg
    16441 tttttactgt caggcaacag aatttccgcc gcctgcaggc gcgccagttc gtcgtccaat
    16501 ttatccacag ttgtcagctt ggttttgaat tcgccgcttt gcagcgatgc ccacgccaaa
    16561 ccgatgtatt ttttgtcggg ggacacggca acgatgcggt tggtttcctt gtcttccagc
    16621 aatgcggaat cggtcagcgt gccgggcgtt acgatgcgca cgactttgcg ctccaccggt
    16681 cctttgcctg cgccgacttc gccgacctgt tcgcaaatcg ccacgctttt gcccaacttg
    16741 accaggcgcg ccagatattg ttcggcggcg tgaaacggca cgcctgccat tttgaccggc
    16801 tcgccatcca cctgtccgcg cgtggtcagg gtaatatcca aaagttttgc cgcttctacc
    16861 gcatcgtcga aaaacatctc gtaaaaatca cccatgcggt aaaacaccag tttgtcggta
    16921 tgttgcgctt tgatgccgag gtattgctgc atcattgggg aaacggcgga tttggacatg
    16981 gggctgcctt atcattagga aaacggtatt gtagcaaaag gttttgtctt cgacggaatg
    17041 agactgaccg taagcctgtc aataaggttg tggagacaag taggaaaaca gcaaagttat
    17101 ccacggagat attattaagt caaaaaacat ccacgtttat ctgtcctata cggcaagcct
    17161 taaactcaaa cttatttgac gaaaaggtaa tgaaaatatt ggatatttgc aacttataca
    17221 caagcagatc cgcaatcatc aaaatcatca ttaaaatttt aaattcaaat attgatggta
    17281 aaagttttgg aaatctgtgc aaacagtaaa attcatctat atcaaataag cggcaagtag
    17341 ttagatgtaa tagtggaatg taactttatt tcagaataca atacaggtat tgaaatttcg
    17401 tttcacagct tggagtatat acatgagcat taaagtagcg attaacggtt tcggccgcat
    17461 cggccgcctc gcattgcgtc aaattgaaaa agcccacgac atcgaagtcg tcgccgtcaa
    17521 cgacctgacc cctgccgaaa tgctgctgca ccttttcaaa tacgacagca cacaaggccg
    17581 tttccaaggt actgccgaat taaaagacga tgccatcgtg gtaaacggca aagagatcaa
    17641 agttttcgcc aatccgaatc ccgaagaatt gccttggggc gagttgggtg tggatgtcgt
    17701 cctcgaatgt accggcttct ttaccaataa aaccaaagcc gaagcacaca tcagggccgg
    17761 cgcgcgcaaa gtcgtgattt ccgcacccgg cggcaatgat gtgaaaaccg tcgtatatgg
    17821 cgtaaaccaa gacattttgg acggcagcga aaccgttatc tccgctgctt cctgcaccac
    17881 caactgcctt gccccgatgg cggcagtcct gcaaaaggaa ttcggcgtag tcgaaggcct
    17941 gatgaccacc atccacgcct acaccggcga ccaaaacacc cttgacgcgc cgcaccgcaa
    18001 aggcgatttg cgccgcgccc gcgccgccgc gctcaacatc gtgcccaaca gcaccggtgc
    18061 cgccaaagcc atcggtctgg ttatccccga attgaacggc aaactcgacg gctccgccca
    18121 acgcgtccct gttgccaccg gctcgctgac cgaattggtt tccgtcctcg aacgccctgt
    18181 aaccaaagaa gaaatcaacg ccgcgatgaa agcagccgcc agcgaatctt acggctacaa
    18241 cgaagatcaa atcgtttctt ccgacgttgt cggcatcgaa tacggctcgc tcttcgatgc
    18301 gacccaaacc cgcgtgatga ccgtgggcgg caaacaattg gtgaaaaccg ttgcctggta
    18361 cgacaacgaa atgtcctata cctgccaact cgtccgcact ttggaatact ttgcaggcaa
    18421 aatctaaggc ttgaacaaac ctgtggataa gtaaatgccg tctgaacaca ttgcgttcag
    18481 acggcattgt ttatctgtat agtggattaa atttaaacca gtacggcgtt ccctcgcctt
    18541 gccgtactat ttgtactgtc tgcggcttcg ttgccttgtc ctgatttaaa tttaatccgc
    18601 tataacttga tattgcggct tatggcgaac aaacaacagc cgtttgttca actgctatgc
    18661 ggcaaaaccg gatttttgcc cgatttaaag cggggttggg gaaagatcaa taacggttgc
    18721 acggttttcc tcgatttgaa atctgacttc gtaatccgca atattcatca cataaatccg
    18781 ttcgggaata tcctgatagg ctgggcgcgg atcttgggca atgctttggc tgataaggtt
    18841 tttggtgttt gcagataaat tttccgcgcc gatgttttcc tgccaaacga cttccaactc
    18901 tacgggtttg ccgctgacga aaccagatgc ggcatcgggt ttggattcga caaaggggat
    18961 ataaggtttg atgtccacaa tcggtgtgcc gtccagcagg tctgcaccgc tgcaatagag
    19021 gcggacgggt ttgccggttt cgatgcgttc gagtttcagg agcgagagtc cgagatggtt
    19081 ggggcggtgg gggctgcgcg tggcgaacac gcccattttt tgtttgccgc cgagccgtgg
    19141 tgggcgcacc atttgcgccc agccttcatc caatacgccg tgaaaaataa aacttatcca
    19201 cacatagtcg aagtgttcca gtccgcgtac gctgtctgcg gtaaattcgg gattcagctc
    19261 gatgcatact tctgcggcag agaccaaacc gggctggcgg gcgatgccga atttctgttt
    19321 gtagggcgat cgggcggtgg cgatagggac gatggtgtaa gtcatgagga ttgtggataa
    19381 gtttttcgga aaaaccttat cttatcacag cctgttttac cgcttttggg cagtaatttg
    19441 ttataataca gggcatttta attgcgttga aatgatgaga tgattgtttc cattgatgtt
    19501 gatgcacaaa aaacttttac gcctttgtgt cctaaagagc tgcctgtaaa cgaggggcat
    19561 ttgattgtcg aagaattgaa tgcccaagcc gctttggcgg atttgcgcgt gatgacgaaa
    19621 gatgcgcatc atgcggcagc aaaatggctt gtggataacc ctgttgatat gttgaagccg
    19681 acaggtttgc ccgatgcgga tttgacttgg gtggctcatg cgatggttgg tacgcgcggc
    19741 tatgaattgt tggacgggct gccttgtgcc aaagaatacg attattgcgt ttggaaaggt
    19801 gttgatcctg aattgcatcc ttacggcgcg tgttttcacg atattgagga aaaactaagc
    19861 acagggctga ttgaatggct gcgttgtcaa aatacggata cggttattat cggtgggtta
    19921 gctacggatt attgtgttaa aacaacggtt ttgcagttac tcaaaggcgg tcgttggcag
    19981 gttgtcgtca atgaagcggc ttgtcggggt attgcgccgg aaaccatcga aacagcatgg
    20041 caggaaatgc attctgccgg ggcaatcatc ttaaaaaacg ctgatgaaat aaataaatat
    20101 attaataatc aataagttaa aattgtttca cgtgaaaccc ccttcctaaa tatgaaaatt
    20161 ttgcttgtcc gcttgtctag tatgggcgat ttaattcaca ctttgcccgc aatcgaagat
    20221 ttggcgcgac aatgtcctga tgtagaactg cattggctat gtgaggctgg atttgcagat
    20281 attgcgcgcc tgcatccgtt tgtaaaaaaa atccatgtga tgaaatggcg gcaatggcgc
    20341 aaacatctct ttcgggctga aacttggcgg gaaatgggtc atctgaaaca gactttgcgg
    20401 caggaagtat ttgatttcgt attggacagt caaggtctga ttaaaagcgc gtgtttcgct
    20461 aaaatggcaa aatccccgat ttgtggtttg gataaaaaca gtgcgcgcga gggatgggct
    20521 gctttggcgt atgtaaaaac atacgctgta ccgaagggaa aaaatgccgt ttggcgcaac
    20581 cgtgaactgt ttgcccaagt atttgggtat gtaatgccgg aaacgcaggt atttggcttg
    20641 actgttcctg aagcaggccg tctgaaaaat ttagcgcagc cgtattatgc ggctttgcat
    20701 gcgaccagtc gggacagtaa gttatggcct gtggaaaact ggcggtcgtt gctgcaaaag
    20761 ttgaatgaag aacagcaatg caatatttac ctgccttggg gaaatgaaga tgaaaaaacg
    20821 cgtgccaaac aaattgcaga cggactgccg tttgctattg tgtgcgacaa aataaattta
    20881 ttgcaggcag cgtatctgct gaaatacgcg gtcggaattg tcggcgtgga taccggtttg
    20941 ctgcatttgg caaatgcctt ggaaaaacct gtggtcggca tttataccga taccgatccg
    21001 attaaaacag gcgttcaggt ttcgccaatt gcaaaaaatt tgggcaatat cgggcagatt
    21061 ccgaccgcag atttggttta tcaagcgctg atggattgtg ttgcagcaga taaaggctaa
    21121 agggtcgtct aaaactgata tggcggcatg atattttcca atttattaga tacaggaatg
    21181 tatatccgct agtctgagta aatgctttaa aaatggtata atcggcaagc attatccgaa
    21241 agatttttga atgaattcgt ttttaaggaa aacggatttg gttttatagg gaagaaggtt
    21301 tggtttcgct ggatggtgaa agcagcgttc tttttaggag ggttcaatga aagcactggt
    21361 cgcagtaaag cgcgtagtgg actacaacgt caaagttcgt gtaaaagccg atggttcgga
    21421 tgtggatatc ggcaatgtca aaatgtcgat gaatccgttt gacgaaatcg ctgtggaaga
    21481 agccgtccgt ttgaaagaag ccggaaaagt aagcgagatc gtagcggttt ctttgggcga
    21541 aaagaaatgc gaagaaacgt tgcgtacagc tttggcgatg ggtgccgacc gtgcaattca
    21601 tgttgaaacc gatgcgaaat tggagccgct ggcagttgcc aagttgctga aagccgttgc
    21661 ggacaaagaa aatccgcaaa ttttcttttt gggtaaacaa gcgattgatg acgatgctaa
    21721 tcaagtggcg caaatgctgg cagctttgtt gaatgcggcg caaggtacgt ttgcttcaaa
    21781 agtacaaatt gaaggtgatg aagtacagat tgtgcgcgaa attgatggcg gcgaagaaac
    21841 catagcattg aaattgcccg ctgttatcag tgcagatttg cgtttgaacg agccgcgctt
    21901 cgtcaaactc cccaatatta tggcggcgaa gaaaaaacct ttggaaaaac tggctcctgc
    21961 cgatttggtt gctgacattt cacctcgtct gaaaacggtg aaattcgccg agcctaaagc
    22021 gcgtcaggca ggcgtaaaag tagcaagcgt tgccgaatta gttgaaaaat tgaaaaacga
    22081 agccaaagtg atttgaggag actgaaatga gcgtattgat tattgccgaa cacgacaaca
    22141 aacagttgaa tcctgccact ttgcatgctg ttactgctgc cgcaaaacta ggcaaagtcg
    22201 atttattggt tgccggaaac ggcgcatcgt ccgtcgtgga gttcgcgaag caagtagcgg
    22261 gagtggaaaa agttttggtt gcagatgctg cccattatgc tgaaggtttg gctgaagagt
    22321 tggctccgct ggttgtcaaa ttggcggcgg attaccgcta tgttgcagca acggcaacca
    22381 cattcggtaa aaaccttttg ccccgcgtag ccgccttatt agacgtaccg caaatttctg
    22441 atttgaccga gattgtagat aacacgactt ttgtacgtcc gatttatgca ggtaatgcat
    22501 ttgaaaccgt gcaagccaat gcggaaaaat tggtgctgac cttccgtgcg acggcttttg
    22561 acgcagcggc ggtgcaaggt ggtaatgctg aggtaattaa tattgaaaca acccctgccc
    22621 aaaacctgag tcgttttgtt aaccgtcagc tttcccattc cgatcgtccc gaactgactc
    22681 aggcaaaagt gattgtttct ggtggtcgtg cgttgggtag tgcggaaaaa tttaatgaag
    22741 tgctgacacc gttggcggat gttttaggtg cggcaatcgg tgcatcccgt gcagcagttg
    22801 atgccgaata tgcgccaaac gatgctcaag tcggacaaac cggtaaagta gttgcgccgc
    22861 aactctattt tgcaatcggt atttcaggtg cgattcaaca cgttgccggt atgcaagaca
    22921 gtaaggtgat tgttgcaatc aataaagatg ctgatgcgcc gattttcaat gtagccgatt
    22981 acggattggt tggcgattta tttgaaatcg ttccacaatt aacagaagca ttgaaaaatt
    23041 aatgtttcac gtgaaacttt cagacgactt tgttattaat gaggttgtct gaaaactttt
    23101 caacatgatt acgacttata aaaccattac ttccccgacg caggctgggt ttaaagataa
    23161 aggcagccgt tttatcgcat ttgcctatcc gattcggaca ttggctgatg tgaaaaaata
    23221 cctcgatccg ttaaaggaag agcaccataa agcacgacac tggtgttatg cctatcgttt
    23281 gggcgtggat ggtgtgcaat ttcgtgccaa cgatgatgga gagccatcag gaagtgctgg
    23341 acgaccaatt ttgggacaga ttgattcggt gggcatcacc gatgttttgg tcgtggtcgt
    23401 ccgctatttc ggtggtactt tattgggcgt ttcaggtctg atacatgcgt acaaagaggc
    23461 gacggctcag gcgttggcga ttgcggaagt gattgaaaag aatattgaaa aaaccgtttg
    23521 gctgaaatgt gaatatccat ttttgaatga agcgatacgc atcgccaaac aatatcaggc
    23581 ggatatattg gagcaggatt tgcagttgga ttgccgattg acggtaagtt tgtcgttggc
    23641 aaattatgaa gtctgtgttg cggcttggaa gaatacacgg cagattgaag taaatacgga
    23701 aaagcctttt gaatgaaagg tcgtctgaaa tgctttgtga tggtatttca gacgacctct
    23761 tcgctgacta atcttcttgt gtatatttgt ctaaggcatc gacgctggag cgcaaatata
    23821 accaagccag ttgatggaat tcgcccagcg cgttccatag cgcatcttca ctcataatgc
    23881 tgtattcgcc ttccgtacgc aaatccagtt ctaccccgtc ttcaatagcc tgatgacacg
    23941 cctgatactc gtgggtatag aaattgtcca tgtcgcgaat taaagtgacg acatatttcc
    24001 aacggcggat gtcttcttcc agttgcggta gtaaatgaca ccattcgcgg tatttgcttt
    24061 ggatttcatc aatacgtttt tgcatggttg cttgtccttt tttaatttat ggataagttg
    24121 aataggttgc agagtggaat gaagcggtct tttgtttgat tctactaaaa tttcttcaga
    24181 tgggataaaa gtccctttgt atcgagtttt cgttaaaagt acatatttgt caaggagatg
    24241 gtatgaaact gctggttatc ggtaatggcg gtcgcgaaca cgcgctggct tggaaattgg
    24301 cgcagtcgcc taaagtggaa acagtatttg ttgcgcctgg taatgccggt acagcgattg
    24361 aacccaagtt gcaaaacatc gacttgactg cgcatcagga tttgattgaa ttcagtcgta
    24421 aagaaaatat tgtttttacc gtcgtcggcc ctgaagcgcc tttggcggca ggtgttgtgg
    24481 atgatttccg tgccgcagga ctaaaaatat tcggtccgac acaatatgcg gcgcagttgg
    24541 aaagttccaa agatttcgcc aaagcattta tggcgaaata caacattccg accgcgcaat
    24601 atcaaacctt tgaaaacgcc gatgccgcac atgattacgt caatcagaaa ggtgcgccta
    24661 tcgttatcaa agccgatggt ttggcggcag gtaaaggcgt gattgtggcg atgactttgg
    24721 atgaagcgca tgctgcgatt gatgatatgc tgctggacaa caaaatgggc aatgccggcg
    24781 cgcgtgttgt gattgaagat ttcctgcaag gcgaagaagc gagttttatc gtcatggttg
    24841 atggcaataa tgtactgcct atggcaacca gccaagacca caagcgtctt ttagacggcg
    24901 acaaaggtct gaatacgggc ggtatgggtg cgtacagccc tgcgcctgtg gtaacgcctg
    24961 ttgtgtacga gcgcgcgatg aatgaaatta ttttgccgac cgtagcaggg atgaaagcgg
    25021 aggggcatga gttcaccggt ttcctgtacg caggtttgat gattgatcaa agcggtgcac
    25081 cctatacgat tgagtttaac tgccgtttcg gcgatcctga aacccagccg attatgagcc
    25141 gactgaacag cgacttatcg gatttggttg aagcggcaat agacggcaga ctcgatagcg
    25201 taaccgcgga atggagcccg caaactgccg tgggcgtggt actggcggcg caaaattacc
    25261 ccgaaacgcc caaaaaaggc gatgttattt ccggtctgga tgctgccaac caagtaggca
    25321 aagttttcca tgcaggtaca acggcaaacg agaaaggtga cgtattgacc aacggcggac
    25381 gcgtattgtg tgttgtggga ttgggcgaca atgtggcgca agcgaaagct aaggcatatg
    25441 gcgcgttgga aaaaatcagt ttcgacggga tgcaataccg taaagacatt gccgataaag
    25501 cgattaaccg ttaactagaa agaggatgat gccgtctgaa gccaagtatg actgatgtgc
    25561 ttcagacggt attttatatt atgttatttc cgagaattat cgcagcatca gcgcaacgta
    25621 agttaagcgt ttatttgaaa aaaggcggtt tggtcgctta tccgaccgaa tcttgttatg
    25681 gcttggggtg tcttccgaca ttagccaaag cactcggtaa gttggcgcat ttgaaaaaaa
    25741 gaccgcaaca caagggtatg attgtcatcg ggaatcaatt tgaacaattg cagcctttac
    25801 ttcaaatgcc gtctgaaaat cttcaggata tgcttagaaa agaatggcct gcccctaaaa
    25861 cgttcctatt atccgccaaa tcctgtgtct tacctgagtt gcgcgggaaa cagcgcagca
    25921 agttggcggt cagggttccc gcccatgtag gtgcgcgccg cttgtgtcag gctttgcaaa
    25981 cgcctttggt ctcgacttcg tgcaaccgcg caggcaaacg cgcgtgtcgg acggagaggg
    26041 aagtcagaag gcaattcgga cgcgatgtgt ggattgttgg tgggagaatc ggcaggcaga
    26101 aatcgccgag tcagattatt gacggggaaa caggtaaacg tttgcgttag atatgggtac
    26161 ttgaatgaaa acttttttgc atacacaaag aaaaaatttt ggggctgtac tagattagcc
    26221 ctaaatccca caccaatcct gtaggatttt tagctgccgg gacagtgtgc cgaagttaaa
    26281 tcgaaattcg cattctttca agaacagcgg gaaagatttg cgatcgattc cattgtattt
    26341 tcgcaagaca cgttttgcct gattccaaaa attctcaatg ccgttaatgt ggttttgacg
    26401 gtctgcaaat tccttggaat ggttgatgcg gtaatggata aaaccgctca cgtccaactt
    26461 gtcgcagctg ctcagactat cggtataaac aatactgtcc ggc
//
LOCUS       DAWFJR010000021        26227 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWFJR010000021 DAWFJR010000000
VERSION     DAWFJR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26227
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.021"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(13..1308)
                     /locus_tag="WGR04_000789"
     CDS             complement(13..1308)
                     /locus_tag="WGR04_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0794378.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMEEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGAQIFEQSPMTCIEP
                     HQNGWLVYTPEGSVECKNVVYAVNTYAGLNPIFRPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPERMTELVRQDMLKVFPQL
                     ADVKIEYSWGGECDITANLVPHFGRLAPNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1434..2321)
                     /locus_tag="WGR04_000790"
     CDS             complement(1434..2321)
                     /locus_tag="WGR04_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0794379.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAH"
     gene            complement(2321..3286)
                     /locus_tag="WGR04_000791"
     CDS             complement(2321..3286)
                     /locus_tag="WGR04_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0794380.1"
                     /translation="MNLNKLKNKLFRRPGQRVVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNIAISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3302..4426)
                     /gene="potA"
                     /locus_tag="WGR04_000792"
     CDS             complement(3302..4426)
                     /gene="potA"
                     /locus_tag="WGR04_000792"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0794381.1"
                     /translation="MTATTASSAKPYLKIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4701..4970)
                     /gene="rpsO"
                     /locus_tag="WGR04_000793"
     CDS             complement(4701..4970)
                     /gene="rpsO"
                     /locus_tag="WGR04_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ0794382.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5193..6128)
                     /gene="secF"
                     /locus_tag="WGR04_000794"
     CDS             complement(5193..6128)
                     /gene="secF"
                     /locus_tag="WGR04_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ0794383.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTKGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6132..7985)
                     /gene="secD"
                     /locus_tag="WGR04_000795"
     CDS             complement(6132..7985)
                     /gene="secD"
                     /locus_tag="WGR04_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ0794384.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALKNAGIQTGGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEMAGGKE"
     gene            complement(8197..8463)
                     /gene="yajC"
                     /locus_tag="WGR04_000796"
     CDS             complement(8197..8463)
                     /gene="yajC"
                     /locus_tag="WGR04_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ0794385.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8591..9664
                     /locus_tag="WGR04_000797"
     CDS             8591..9664
                     /locus_tag="WGR04_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ0794386.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACTGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKIFRL
                     DNDTAISTGSLSAAHFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDKSG
                     RLNLHEADFGWLTHKIIQTASGCPGKVVSVLEGGYTLESLSKSAAEHIRVLAGLGKSE
                     TAAAYQAELDHNREHVPPDPNPA"
     gene            9919..10983
                     /locus_tag="WGR04_000798"
     CDS             9919..10983
                     /locus_tag="WGR04_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ0794387.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVVWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEDVDDIKVGQHVVVEPYIVHD
                     DVPTGPGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            11124..11447
                     /locus_tag="WGR04_000799"
     CDS             11124..11447
                     /locus_tag="WGR04_000799"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ0794388.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            11519..11842
                     /locus_tag="WGR04_000800"
     CDS             11519..11842
                     /locus_tag="WGR04_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ0794389.1"
                     /translation="MDNCIFCKIAAKDILAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            11889..12092
                     /gene="tatA"
                     /locus_tag="WGR04_000801"
     CDS             11889..12092
                     /gene="tatA"
                     /locus_tag="WGR04_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ0794390.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            12096..12782
                     /gene="tatB"
                     /locus_tag="WGR04_000802"
     CDS             12096..12782
                     /gene="tatB"
                     /locus_tag="WGR04_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ0794391.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMEGNLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPFPDAANTLLDGISDVMPSERSYASAETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKSR
                     AKPKLRVRKS"
     gene            12796..13566
                     /gene="tatC"
                     /locus_tag="WGR04_000803"
     CDS             12796..13566
                     /gene="tatC"
                     /locus_tag="WGR04_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ0794392.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGMMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKISVVTTGQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            13619..14224
                     /gene="maf"
                     /locus_tag="WGR04_000804"
     CDS             13619..14224
                     /gene="maf"
                     /locus_tag="WGR04_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ0794393.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYQVVKLPADINETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAATFLNLL
                     SGTGHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            14240..14593
                     /locus_tag="WGR04_000805"
     CDS             14240..14593
                     /locus_tag="WGR04_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ0794394.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            14583..16397
                     /locus_tag="WGR04_000806"
     CDS             14583..16397
                     /locus_tag="WGR04_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ0794395.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALKLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACKSLVAHFGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     LQNLIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPPALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVLLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIHDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            16587..17264
                     /locus_tag="WGR04_000807"
     CDS             16587..17264
                     /locus_tag="WGR04_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0794396.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            17277..18683
                     /locus_tag="WGR04_000808"
     CDS             17277..18683
                     /locus_tag="WGR04_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ0794397.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIVVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            18801..21227
                     /locus_tag="WGR04_000809"
     CDS             18801..21227
                     /locus_tag="WGR04_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ0794398.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHSLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTVRALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALEAADPALIAEAL
                     HLPPYRHTTHNAVQFQFGSHSLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTIIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     YPKTLSLRNNTTVTIIPLLPEDAEAKQQFVRSLGPEARYTRFMTHTNELPTATLARLC
                     NPDYHCEAAWTARDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLHTDHKIT"
     gene            21272..21517
                     /gene="rpsP"
                     /locus_tag="WGR04_000810"
     CDS             21272..21517
                     /gene="rpsP"
                     /locus_tag="WGR04_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ0794399.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            21533..22042
                     /gene="rimM"
                     /locus_tag="WGR04_000811"
     CDS             21533..22042
                     /gene="rimM"
                     /locus_tag="WGR04_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ0794400.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTVSVTLEAGKVVNGELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            22042..22791
                     /gene="trmD"
                     /locus_tag="WGR04_000812"
     CDS             22042..22791
                     /gene="trmD"
                     /locus_tag="WGR04_000812"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ0794401.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHPKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            22808..23173
                     /gene="rplS"
                     /locus_tag="WGR04_000813"
     CDS             22808..23173
                     /gene="rplS"
                     /locus_tag="WGR04_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ0794402.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            23492..24217
                     /locus_tag="WGR04_000814"
     CDS             23492..24217
                     /locus_tag="WGR04_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ0794403.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFR
                     GINAVQKQRVHESLERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPSTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPDWCAS"
     gene            24252..25127
                     /locus_tag="WGR04_000815"
     CDS             24252..25127
                     /locus_tag="WGR04_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ0794404.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSSLTLITLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            25204..26118
                     /locus_tag="WGR04_000816"
     CDS             25204..26118
                     /locus_tag="WGR04_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ0794405.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSSDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEFDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         6766 a         7856 c         6069 g         5536 t
ORIGIN      
        1 tgcctgcgac gctcaacggc tgtctttcca ctgataatat ttcgagccga ggaaagaacc
       61 gagtttgcgc aaaaacggtt gcaggataat attcggctgg cgcaaccgct caaacggctt
      121 cagacggcat tcgtccccta aaattgcttc ggcaaccgcc agacctgcaa tgcctgttat
      181 cgccatcccg tgtccggaat aaccttgcgt ataaaaaaca ttcggggcta aacgtccgaa
      241 atgcgggaca aggttggcgg taatgtcgca ctccccgccc cacgaatatt cgattttgac
      301 atcggcaagc tgcggaaaaa ctttaagcat atcttggcgg acaagctcgg tcatacgctc
      361 aggattgtcg ataaactcgt tatccttacc gccgaaaagc agtctgccgt ccgcgctgag
      421 gcggtaataa tccaaaatat ggcggttgtc gcatactgcc atattgttac ggataagccc
      481 ttttgcgcgc gcccccaagg gttcggtcgc aataataaag gtgctgacag caatcgcctt
      541 gcgttccaaa ggccggaata tcgggttcaa acctgcataa gtattgacag catagaccac
      601 atttttgcac tcgacgctgc cttcgggcgt gtaaaccagc caaccgtttt gatgcggttc
      661 gatgcacgtc atcggggatt gctcgaaaat ctgcgcaccg gcttcggcag cggcacgagc
      721 gatgcccaaa gtgtaattga gcggatgcag gtgtccggat aaggggtcga attgtgcccc
      781 ttggtacata tcgctgtcaa gctgctgttt caactcggct ttatcccaaa gttgataatg
      841 actcgcaccg taatgccgtt gggcgtgttc atgccactgc tgcaactctt cccaatgctg
      901 cggacggacg gcaaccgtgg cataaccgcg ctgccaatcg caatcaatgg catgtttgcg
      961 gacgcgttcg tccaccagtt cgaccgcctg caaagactgt tgccaaaacc attgcgcctg
     1021 ctccaagccg acctgttttt caatttcctc cataccgcag gcgtagtcgc tgataacctg
     1081 accgccgctc ctgccggacg cgccgaagcc gatacgcgcg gcttccaaca caaccgtttc
     1141 atgtccctgc tccgccaagg gcaatgcagt gcacaaacca ctcaatccgc cgccgatgat
     1201 acaggtatcg gttttcagac ggcattgaag tttcggataa acagtatgag gattaaccga
     1261 actaaaataa taagaaggca gatattcttg aaaatcagga cgaatcatta tgtttacttt
     1321 atcaggtgta ttttcagacg gcatgatatg gtttgccgta ccatgccgtc tgaacgggaa
     1381 attactttaa gaaaacaggc tgacctgata atgcggtcag cctgccctgt cggtcaatgc
     1441 gctttctcag cagccaattt ttcttggcgg taggcttctg ccgcttctcg gtcgcgcttg
     1501 gttgcctgcc tcatcatcca ataattgacg atgatgacca atgttccgat gatgccgatt
     1561 aggatggtcg ccaagacatt catctgagga tcaagaccca gcttgatttt ggagaaaatc
     1621 acctgcggca atgtggatga accggggccg gagaggaatg aggtaattac caaatcatcc
     1681 aaagacaggg taatgccgag cagaaagcct gaagcgatgg caggggcaat caaaggcaaa
     1741 gtgatgacaa aaaagatttt cagcggacgc gcgcccaaat ccattgcggc ttcttcgagc
     1801 gattggtcaa gctcaaccag acgcgaacgg ataacaacgg taatgtacgc catacacagc
     1861 gtcgtatgtc cgaggaagat ggtgaaaaag ccacgatcga agtagagatg ttgtaaccat
     1921 tcgctgccct gcaaaaatat ctgtacctga ataatcagca gcagcataga cagaccggta
     1981 atcacgtcgg gcatcaccat aggtgcggaa atcatgccag cgaacaaggt actgccgcga
     2041 aaacgtttaa tccgcgccat cgcatagcct gccagcgtgc ccaaaacgac ggcggcaagc
     2101 gaagacacaa cggcaatccg cagcgacagc caagcggctt ccaagatggt gtcgttttcc
     2161 agcaatgcgc cgtaccactt ggtcgaaaag ccgccccaaa cggttaccag cttggattcg
     2221 ttaaacgaat agatgaccaa aacaaccagc gggatataca gaaacgccag cgacagtgcc
     2281 aacatcagtt tcaagaacca agataatttg gatttctgca ttatttggct ccttcttcca
     2341 attcgcggtt ttcataatgc tgaaacaggg caatcggcac gaccagcagc gcgaccatca
     2401 cgacggcgac ggcggaagcc agcggccagt tgttttgatc gaagaacgcc tgccacaaga
     2461 ctttaccaat catcaggttt tccgaaccgc cgaccagctc gggaatgacg aactcgccga
     2521 cagcagggac gaaaaccagc atggagcctg caataatgcc ggttttcgac aaaggcaggg
     2581 taatcgtcaa gaacgatttg accggccccg cgcccaaatc ggaagccgct tcaagcaggc
     2641 ggttgtcgag tttcaccagt tgcgtgtata gcggcagaat cataaacggc agataggcgt
     2701 aaaccatcac caaattgagc gaaaaggcat tgtagaacaa atccaaaggc tcgctgataa
     2761 tacccatttt aatcaacagg ttgtttacaa tgccgttatg cccgagcaga cccatccacg
     2821 catagacgcg caacaggaac gatgtccaaa agggcagcat aatggcaagc agcaaaccat
     2881 tgcggacaga aggattggca cgagaaatcg cataggcggt cggataaccg accaacaggc
     2941 aaatcactgt cgtcatcaat gcggttttga ttgaagacca gtaggtcatc agatagatat
     3001 tgctgttttc actgtcgccg aacgggttaa gggtatgcca aaaattttgg aaaatatcgg
     3061 catagttttg ataactgatg gcgatattca gacgacctaa atcttcgtcg atgctggtca
     3121 gcggcgtaaa cggcggaatg gcgatttctt gttcggcaaa gctgattttc agcacgatgg
     3181 cgaacggaat cagaaacagc accaaaagcc aaatatacgg tacggcaatc accacacgct
     3241 gccccgggcg gcggaacagt ttgtttttca gtttattaag gttcattgca ttccccttaa
     3301 atcaacggaa cagtggcgta ggctggtttt ccggccagct gatatacacg gtttcgtccc
     3361 aagttggcgg tgtaatgttg cgtacatacc agtaaggtgc aggaacttgg cttttgacga
     3421 cgcgcccgtt ggcgagtttg atatggtata tggcaaagct gcccaaatag gcgatttctt
     3481 ttaccgtgcc tttcgcccag ttgtagtcgc ccaaatattc gggtttttct ttatataaat
     3541 caatatcctc tggtcgaata ctaacccaaa ggtcctgctc gctcggacca cccaaaccgt
     3601 gatcgatgcg gacgtggttt tccaaacctt cgcattcgat aacggcatag tcggcatgat
     3661 cttcaatcac cacaccgtca aagatgttgg tttcgccgat aaactcggca gtgaagcggc
     3721 tgttgggata gtcgtacacg tcgctgggtg tgccgacttg ctgcaactga ccgtcagaca
     3781 taatggcgat gcgggtcgcc atcgtcatcg cctcttcttg gtcgtgcgta accataatac
     3841 aggttacgcc gacttgttcc agcgtattga ccaactcaag ctgggtttgt tggcgcagtt
     3901 ttttgtccaa tgcaccgagg ggctcatcca gcagtagaat tttcggacgt tttgccagac
     3961 tgcgtgccaa agcaatgcgc tgctgctgac cgccggacaa ttggtgcggt ttgcgtttag
     4021 caaatttggt catctgaacc aggcggagca tttcttcgac gcgcgcggcg atttcgcctt
     4081 taggcatttt gtcctgtttc agaccgaagg caatgttttg ttctacggtc atatgcggaa
     4141 aaagcgcgta actttggaac atcatattga tggggcgatc atagggtgca agtttggtaa
     4201 tatcctgacc atcaaggata atttttccct gattgggact ttccataccc gccagcatac
     4261 gcagcagtgt agattttccg ctgccggaac tgcccaaaag ggcgaagatt tcgtgttgat
     4321 aaatgtccaa gtcgatgtta tcgacagcgt aattgtcacc aaactttttc accaaacctt
     4381 ggattttgag ataaggtttg gctgaagacg cagtggttgc ggtcataatg gcaatactcc
     4441 aataaaaaga cgagtaccgg caaaacggat tttcgaatgg gtgataaaaa gctgtttgat
     4501 tgctggcggg agttaaacgt ttgatgccgt ctgaaactct tgtaaagcgc acgggcagca
     4561 tgaaatggaa caagattcca aagaacttta tattatatta gtttatgcgg ttttcgggca
     4621 atatagtgga tttgagcttg tcggaaagca acagaaaaga aaacaccgcc catttttctg
     4681 ggcggtgtcg gaaagcgtaa ttatttacgc agacccaagc gggtaatcaa cgcgcgatac
     4741 gtatcgggct gggtacggcg caagtaggcc agcaggcggc ggcgttggct gaccattttc
     4801 aacaggccgc gacggctgtg gtggtctttg gggttggctt tgaagtgggg ggtcaggtcg
     4861 ttgatgcgga aagtcaacag agcgacttgt acttcggaag agccggtgtc gccttctttg
     4921 cgttggaaat ctttaacgat ttgtgctttt tgttctacgg tcagtgccat aatgaaaact
     4981 ccaaaaatat aagaatcccc atagggattc agacaagttt gccaagcctg aagacaacgg
     5041 caaactccct atgcccaaga taggacaacg tggcattatg acacaattcc cgcatttctg
     5101 cacaatattt taagaccgtg gaagtaaccg ttaaaaatgc cgtctgaagc attgtctgct
     5161 tgagacggca atgttcagac ggcatatgcg cttcaaacca cgatttcttc tttcttcttc
     5221 ggttctttac cgatattgtc gcggctcaaa ccgaacatta gcagaagcgg gctggcaacc
     5281 aatacggaag aataaatgcc gaacacgatg ccaatggtca acgccataga aaagccgtgc
     5341 aaggccgcac cgccgaacac cagcatagaa acaaccatcg cctcggtcga accgtgggta
     5401 atgatggtgc ggctcatcgt tgcggtaatc gcgttgtcga tgacttccgg cacggcatgt
     5461 ccgcgcatcg ccggcttgcg gaagttttca cggatacggt cgaagacgac gacggattcg
     5521 ttcacagaat agcccaatac ggcaaggata cccgccaaga cggtcagcga aaattcccat
     5581 tggaagaagg caaagcagcc gagaataatc acgatgtcgt gcatattggc gataatggca
     5641 gatacggcaa aacgccattc aaaacgcatc gacaggtaaa taatgatgcc gataacgaca
     5701 aaacctaaag ccatcaatcc attacttacc aattcctcac cgacttgcgg gccgataaat
     5761 tcgacttggc gcaaggtaac gtcgggactg tcttttttca gcaaatccat aacctgattg
     5821 gacaactgtg cggaagtaac accttctttg ttcggcaggc ggatcatgat gtgtttgttc
     5881 gtacccaatg cctgaacctg tacatcacct attttcagcg tatcgaggcg ttcgcgcatc
     5941 ttattgacat ccgcaccctg ctgatattgg acttccatta ccgtaccgcc ggtaaattcg
     6001 acagagaaat tcaggccttt ggtcaccaag aagaacacgg cagcgataaa cgtaaccaac
     6061 gaaatgaagg tcgtcagttt gccgtagctc ataaacggaa tatcgcgttt gattttaaag
     6121 agttccatag cttactcctt gcctcctgcc atttcggctt tcggcttcca caccgaacca
     6181 atggaaatat tctgcaattt gcgtctgcgt ccgtaccaca gattgaccaa cgcacggaat
     6241 acgacgacgg aggaatacat cgaagtcaga atacccaaac agtgtacgac cgcaaaaccg
     6301 cgtaccgggc cggaaccgaa taccaaaagc gcgataccgg caatcagcga agtcaggttg
     6361 gaatcgacaa tggtcgccca tgcgtgttgg aaaccgagat tgattgcctg ctgcggcggc
     6421 acgccggcac gcaattcttc gcggatacgt tcgttaatca agacgttgga gtcgattgcc
     6481 atacccaaag tcaacgccag cgcggccata cccggtaacg tcaacgttgc ctgcatggca
     6541 gacaaaatac cgattaggaa cagtatgttg gcactcaatg caatggtaga aaagaaaccc
     6601 atcagacgat agtaaaccac catgaatgca gcaacgatgg caaaacccca taaagtcgaa
     6661 tggaagcctt tttcgatgtt ctccttaccc aaagacggac cgatggtacg ttcttcgaca
     6721 atctgcatcg gcgcggcaag agaaccggca cgcaacagca aagacgtatc attggcttcg
     6781 gctgtcgtca tgcttccgga aatttccacg cgtccgcctg taatggcagt acggataacc
     6841 ggcgcggtta caacctcgga ttttccttgg tcgatcaaaa ccatcgccat gcgtttgccg
     6901 acatttgcgg cagtcagttc gccgaaaatg ctgccgcccg cgctgtccaa gctcagactg
     6961 acggcaggtg cgcccatttg gtcgaaactc ggttgcgcat cgttgatgtt gtcgcccgtc
     7021 agctcgacct gtttgctgat tagcagagtt tcgggacgat ctccgccgct tgaaagcagc
     7081 tcataaccgc tcggcacgtt gccttccaat gcctcgcgca acttggcagg atcgtcctcc
     7141 accatacgca attccaaagt cgcggtacgg ccgatgatgt cttttgcctt ggcagtatcc
     7201 tgaacgcccg gaagctgcac gacgatacgg tctgcacctg actgctggat gacgggttcg
     7261 gccacgccca actcgttcac acggttgtgc agggtagtga tgttctgttt gaccgcatcg
     7321 gaacgcactt tattgaccgc ctcttccgaa agcgtcaaga cgatattgct gccgtctgaa
     7381 ttcagcgttg cttcaggaaa cagcttgcgc aactgcggca gtgccttttg cacatcacct
     7441 gcatcctgca aagggacggt caggctgttt ccagcctgac gcaccgtgcc gctgcggatt
     7501 ttttcgcggc gcagttcgcg gcggatgtcg cccgaataac gttcaaacgt tttctgcatc
     7561 gccgctttca tatcgacctg catggtgaaa tgcacgccgc cgcgcaagtc caaacccaaa
     7621 aacatcggat tggctttgat tttcgccatc cattcggggc tgtccgccaa caggttgagc
     7681 gcggtaatat acccttcgcc caaagtgttt tcgatgacgt cgcgcgcttt aagctgcgtt
     7741 tctgtgtctt tgaaacgcac tttcagtgaa ttgtccacaa caaacatccc gccggtctga
     7801 atacctgcgt ttttcagcgc ggcatccact ttggattgag tctgttcgtt gatgatgatg
     7861 gcttgtcggt tggtcgatac ctgcacggcg ggtgtttcgc cgaataggtt gggcagcgaa
     7921 tacactgcgg caaccgcaat cgtgaacaca atcagcagat atttccataa aggataacgg
     7981 ttcatcattg ttccttaatg gttggaaccc caccctttcg gtggtgtcgg aatcgggcta
     8041 tttcagaaga ggcaaaaacc cttcccagcc aggcaagacc ggaaggcggc atcttgaata
     8101 tgccgccctg cgtgtcggaa catggtcaag ccttcggttg gaattcaaaa caaagtgccg
     8161 cattcgggct ttccagatgc ggcttgtcgg cacaaatcaa tcgacttttg cggcaatcgc
     8221 attgcgttcc acttcgacct cgatttttgt accctgtccg atatccacgg taaaaaactg
     8281 ttcgccgact ctggttacct tacccttgaa acctgccgcc aagaccactt tgtcgccgac
     8341 tttcaaggcg gcaagcattg cctgatgcgc tttgaatttc ttttgctgcg gacgcatgat
     8401 caggaagtag aacaccacca taatcaacac taaaggagca aattgtgcaa cagcttgatt
     8461 cataatttat ccgttctttc taatatggtt gaaaatcgag aggggtatat aataacataa
     8521 gaccgtaaac aatatatcgg gtttgccgtc cgtaccgacc gtataccgca gcctgcccgt
     8581 ccacaaaccc atgtccttga cccgcctaat cttgaaattc tatgcactat tgcgcctctt
     8641 tttgggcaaa aacgcccgca ccgcatggat ttcgcatccc gcctgcacag ggcacgaacc
     8701 cggcgcaaac catcccgatt cgcccgaccg catcctctgc atcgagcagg cattgcgccg
     8761 cgccggtatt tggcagcacc tccaaaccat agaggcggaa gaaatcagcg atacgcgcct
     8821 cgcgcttgtc cactcgagca aatatctgaa ccgtttggaa tcttgcctgc cccaaaaagg
     8881 caagattttc cgcctggata acgacactgc aatcagcaca ggctcgctgt ctgccgcaca
     8941 ctttgccgcc ggttcggcag ttcaggcagt cgacatggtc atgaaccgta aagcatggca
     9001 tgccttttgc gccgcccgcc cgcccggaca ccatgcgggc agcggcaaag ccggcggatt
     9061 ctgcctgctg aacaacgttg ccgccggcgt catgcatgcc attgccgaat accgcctgaa
     9121 acgcattgcc gtcatcgatt tcgatgtcca ctacggcgac ggtacggcag aaatattcaa
     9181 agacgatccg cgcatcctgt ttttcaacct gtttgaaacc gacctttttc ctttccccga
     9241 aaacaacgat atgcccgacg gcggcaatat ggtgcacctg cccttgccgc cgggaacggg
     9301 cagccgcaca ttccgcgaag ccgtccgcag acagtggctg ccccggcttg ccgcattcaa
     9361 acccgaactg gtgctgctgt cggcaggatt cgacgcacac cgtctggaca aatcgggcag
     9421 gctcaacctg cacgaggcgg atttcggctg gctgacacac aaaattattc aaacggcatc
     9481 gggctgcccg ggtaaggtcg tatccgtgct ggaaggcggt tatacccttg aatccttatc
     9541 aaaatccgcc gccgaacaca tccgcgtgct ggcaggattg ggaaaatccg aaacggccgc
     9601 cgcctatcag gcggaattgg accacaacag ggaacacgtc ccgcccgatc cgaacccggc
     9661 ataagcacgc cggaccggcc gttttaaaac aaaggctatg acggcatagg tattatttaa
     9721 atccgattcg gtttgcaggc ggtatcatta tttgacctga agcaaaacaa gaaactttta
     9781 tctgcttgaa ataggttttt tgtagccaaa taacacttgg gcagattgaa tccggctctt
     9841 accggccgcc gtttccatcc caaccgccgt ccgacatttt tcagacggca ttcctacttg
     9901 aaaacaagga gcgttacaat gaaagcagca cgtttttacg acaaaggcga catccgcatc
     9961 gaagacatcc ccgaaccgac cgtcgccccc ggcaccgtcg gcatcaatgt tgtctggtgc
    10021 ggcatctgcg gtaccgacct gcacgaattc atggaaggcc cgattttcat tccgccttgc
    10081 ggtcatccgc acccgatttc cggcgagtcc gcacccgtca cgatgggaca cgagttctcc
    10141 ggcgtggtct atgccgtcgg cgaagacgtg gacgacatca aagtcggcca acacgtcgtg
    10201 gtcgagcctt acatcgtaca cgacgacgta ccgaccggcc cgggcagcaa ctaccacctc
    10261 tctaaagaca tgaacttcat cggcttgggc ggctgcggag gtggcttgtc tgaaaaaatc
    10321 gctgtcaaac gccgttgggt gcatccgatt tccgacaaaa tcccgttgga tcaagccgct
    10381 ttgatcgaac cgctgtctgt cggacaccac gcctatgtac gcagcggcgc gaaagcaggc
    10441 gacgtcgcat tggtcggcgg cgcaggtccg atcggtttgc tgttggctgc cgtgttgaaa
    10501 gccaaaggca tcaaagtcat catcaccgag ttgagtaaag cacgcaaaga caaagcgcgc
    10561 gaatccggcg ttgccgacta catcctcgac ccgtccgaag tcgatgttgt cgaagaagtg
    10621 aaaaaactga ccaacggcga aggcgtggac gtggcattcg aatgtaccag cgtgaacaaa
    10681 gtgttggata ctttggtcga agcctgcaaa cctgccgcca atttggttat cgtatccatc
    10741 tggagccacc ctgcaaccgt caacgtccac agcgtcgtga tgaaagaact ggatgtgcgc
    10801 ggcacgattg cctactgtaa cgaccacgcc gaaaccatca aactggtcga agaaggcaaa
    10861 atcaacctcg agcctttcat cacccagcgc atcaagctgg acgaactggt ttccgaaggc
    10921 ttcgagcgtc taatccacaa caacgaatcc gccgttaaaa ttattgtgag tccaaacctg
    10981 taagcaaatt acatttgcaa cagcacaaat gccgtctgaa acgctcagac ggcattttcc
    11041 aacgaagcgc aacaggcgta ttgcccgaga gcagccgttt agcctatcat tccgaacaaa
    11101 cagaaacaaa cagaaggaaa atcatgggag attccgtact atccgccatc caacaaacca
    11161 tcacccagcg aaaatctgcc aatccgtccg aatcttacgt cgcacagctc ttgcataagg
    11221 gcgaagacaa aatcctaaaa aaagtgattg aagaggcggg cgaagtgttg atggcatcca
    11281 aagacaaaaa cccgtcccac ctggtttacg aagttgccga cttatggttt cacaccatga
    11341 ttcttctgac acaccacgac ctgaaggcgg aagacgtatt ggacgaactt gcgcgccgcc
    11401 aaggtttgtc gggcttggcc gaaaaagccg ctcgcacaga atcttgaatt tatattaaaa
    11461 tccgcacttt cccacattca atccgtctga ccgcttttca gacggcatcg gagccgttat
    11521 ggacaactgt attttctgca aaatcgccgc caaagacatt ctggcgcaaa ccgtctatga
    11581 agacggcgaa atggtttgtt tcaaagacat caaccccgct gctccggttc atctgctgct
    11641 gattcccaaa gtccatttcg attcgttggc acacgccgcg cccgaacatc agcccctttt
    11701 gggaaaaatg atgctgaaag ttcccgaaat cgccaaagcg gcaggactgg cagacggctt
    11761 caaaaccctg atcaacaccg gaaaaggcgg cggacaagag gtcttccacc tgcatataca
    11821 catcatgggc acacccgtat aaaccgttat ttcacaatca acccctaata cttacttaag
    11881 gatacatcat gggcagtttt tctctgacgc actggattat cgtactgatt atcgtcgttt
    11941 tgatattcgg caccaaaaaa ctgcgcaacg tcggcaaaga cctcggcggt gcggttcatg
    12001 acttcaaaca ggggctgaac gaaggcacag acggcaaaga agcccaaaaa gacgatgtaa
    12061 tcgaacacaa aaaagacgaa gacaaagcgt aatttatgtt tgatttcggt ttgggcgagc
    12121 tggtttttgt cggcattatc gccctgattg tcctcggccc cgaacgcctg cccgaggccg
    12181 cccgcaccgc cggacggctc atcggcaggc tgcaacgctt tgtcggcagc gtcaaacagg
    12241 aatttgacac tcaaatcgaa ctggaagaac taaggaaggc aaagcaggaa tttgaagctg
    12301 ccgctgctca ggttcgagac agcctcaaag aaaccggtac ggatatggag ggtaatctgc
    12361 acgacatttc cgacggtctg aagccttggg aaaaactgcc cgaacagcgc acgcctgctg
    12421 atttcggtgt cgatgaaaac ggcaatccct ttcccgatgc ggcaaacacc ctattagacg
    12481 gcatttccga cgttatgccg tccgaacgtt cctacgcttc cgccgaaacc cttggggaca
    12541 gcgggcaaac cggcagtaca gccgaacccg cggaaaccga ccaagaccgt gcatggcggg
    12601 aatacctgac tgcttctgcc gccgcacccg tcgtacagac cgtcgaagtc agctatatcg
    12661 ataccgctgt tgaaacccct gttccgcata ccacttcgct gcgtaaacag gcaataagcc
    12721 gcaaacgcga tttgcgtccc aaatcccgcg ccaaacctaa attgcgcgtc cgtaaatcat
    12781 aaagagagca atccggtgtc cgaaacacaa aacgaacaac ccgtccaacc gcttgtagag
    12841 catctcatcg agctgcgccg ccgcctgatg tggacggttg tcggcatctt agtctgcttt
    12901 ttcggcatga tgccgtttgc ccaacaactc tatactttta tcgccgaccc gctgatggca
    12961 aacctgccca aagacaccag catgattgcc accgatgtca tcgcaccatt tttcgtgccg
    13021 gtcaaagtta ccctgatggc ggcattttta atttcgctgc cgcatacgct ctaccaaatc
    13081 tgggcatttg tcgcgcccgc actctaccaa aacgaaaaac gcctgattac gccgctcgtc
    13141 ctctccagcg tcagcctgtt tttcatcggc atggcatttg cctacttttt ggttttcccc
    13201 gtcattttca aattccttgc cagcgttacc cctgtcggtg tcaatatggc gacagacatc
    13261 gacaaatacc tgtcttttat cttaggcatg tttgttgcgt tcggcacaac gtttgaagtt
    13321 cccatcgtcg ttatcctgtt aaccaaaatc agcgtggtaa caaccggaca gctcaaacgc
    13381 gcccgcccct atgtgattgt cggcgcgttt gtcattgccg ccatcatcac gccgcccgat
    13441 gtgatttcac aaaccctgct tgccattccg ctgattctct tatacgaagc aggtatttgg
    13501 ttcggacgct ttttcacgcc acgttcagaa caggatggcg acatacagcc gcctgcaaca
    13561 acctgacact atgccgtccg aacctccgcc tcataccgca acagattaag gaataccttt
    13621 gaataccctc tatttaggtt caaacagccc gcgccgaatg gaaatcctga cacagttggg
    13681 ctatcaggtc gtcaagctgc ctgccgacat caatgaaacg gtcagacaga acgaagaccc
    13741 tgcccgttac gttcaaagga tggcagaaga aaaaaaccga accgccctga ccctcttttg
    13801 cgaaaccaac ggcacaatgc ccgatttccc cctgattacc gccgacacct gcgtcgtttc
    13861 agacggcatc atattgggca aaccccgctc ccaagctgaa gcggcaacct ttctaaacct
    13921 gctgtcggga accgggcata ccgtcctgac tgctgtctgc attcattatc gcggcaaaac
    13981 gtcaagccgc gtccaaacca accgcgtcgt tttcaagccc ctgagttcgg aagaaatttc
    14041 cgcctatgtc caaagcggcg aaccgatgga caaagccggc gcctacgccg tacaaggcat
    14101 aggcggcatc tttatccaat ctatcgaagg cagcttcagc ggcattatgg ggctgcccgt
    14161 ttatgaaacc gtttcgatgt tacaggattt gggataccgt accccacctt tcatccgagc
    14221 ctaacccgta aagaccgccg tgaacagaca aaccgcttac ctccttacct ctttcagcct
    14281 gatcgcactg ataatcctgt ccctttcctg ggaactgtgg attgcaccgt tgcgcccggg
    14341 cggctcgtgg ctcgcgctca aagccctccc cctctgtctg ccgctttcag gcatcttgaa
    14401 aaagaaaatc tatacctacc aatacagctc catgctggtt ctgatttact ttgccgaagc
    14461 cgtcatgcgc ctgttcgacg cctatcccgc agaacagatt tgcgccgcgc tttccgcagt
    14521 attcagcatc atcttcttca tatcctgcct gtcgttcgtc aaacaataca aggaaacaaa
    14581 caatgcccgc tgaaacgacc gtatccggcg cgcaccccgc cgccaaactg ccgatttaca
    14641 tcctgccctg cttcctttgg ataggcatcg tcccctttac cttcgcgctc aaactgaaac
    14701 cgtcgcccga cttttaccac gatgccgccg ccgcagccgg actgattgtc ctgttgttcc
    14761 tcacggcagg aaaaaaactg tttgatgtca aaatccccgc catcagcttc cttctgtttg
    14821 caatggcggc gttttggtat cttcaggcac gcctgatgaa cctgatttac cccggtatga
    14881 acgacatcgt ctcttggatt ttcatcttgc tcgccgtcag cgcgtgggcc tgcaagagtt
    14941 tggtcgcaca cttcggacaa gaacgcatcg tgaccctgtt tgcctggtcg ctgcttatcg
    15001 gctccctgct tcaatcctgc atcgtcgtca tccagtttgc cggctgggaa gacacccccc
    15061 tgcttcaaaa cctcatcgtt tacagcgggc aaggcgtaat cggacacatc ggacagcgca
    15121 acaacctcgg acactacctg atgtggggca tactcgccgc cgcctacctc aacggacaac
    15181 gaaaaatccc gcccgccttg ggcgcaatct gcctgattat gcagaccgcc gttttaggtt
    15241 tggtcaactc gcgcaccatc ttgacctaca tagccgccat cgccctcatc cttcccttct
    15301 ggtatttccg ttcggacaaa tccaacaggc ggacgatgct cggcatagcc gcagccgtac
    15361 tcctaaccgc gctgttccaa ttttccatga acaccattct ggaaaccttt accggcatcc
    15421 gctacgaaac tgccgtcgaa cgcgtcgcca acggcggttt cacagacttg ccgcgccaaa
    15481 tcgaatggaa taaagccctt gccgccttcc agtccgcccc gatattcggg cacggctgga
    15541 acagttttgc ccaacaaacc ttcctcatca atgccgaaca gcacaacata cacgacaacc
    15601 tcctcagcaa cttgttcacc cattcccaca acatcgtcct ccaactcctt gcagagatgg
    15661 gaatcagcgg cacgcttctg gttgccgcaa ccctgctgac gggcattgcc gggctgctga
    15721 aacgccccct gacccccgca tcgcttttcc taatctgcac gcttgccgtc agtatgtgcc
    15781 acagtatgct cgaatatcct ttatggtatg tctatttcct catccccttc ggactgatgc
    15841 tcttcctgtc ccccgcagag gcttcagacg gcatcgcctt caaaaaagcc gccaatctcg
    15901 gcatactgac cgcctccgcc gccatattcg caggattgct gcacttggac tggacataca
    15961 cccggctggt taacgccttt tcccccgcca ctgacgacag tgccaaaacc ctcaaccgga
    16021 aaatcaacga gttgcgctat atttccgcaa acagtccgat gctgtccttt tatgccgact
    16081 tctccctcgt aaacttcgcc ctgccggaat accccgaaac ccagacttgg gcggaagaag
    16141 caaccctcaa atcactaaaa taccgccccc actccgccac ctaccgcatc gccctctacc
    16201 tgatgcggca aggcaaagtt gcagaagcaa aacaatggat gcgggcgaca cagtcctatt
    16261 acccctacct gatgccccga tacgccgacg aaatccgcaa actgcccgta tgggcaccgc
    16321 tgctacccga actgctcaaa gactgcaaag ccttcgccgc cgcgcccggt catccggaag
    16381 caaaaccctg caaatgaccc gcgccggcgg atgcggatac cgcccgaaat gtaaagctcc
    16441 atgcaagaca ttgcaaaaaa cagcaaaccg gtagggaaat acgctatcaa aaaacattgc
    16501 agccgtgtta agataaaccg tcaaacaatc ttttcacgcc ccgcccgaaa cagggtcggg
    16561 gcataccctt acgaaaagga aacaccatga gccgcgtatt actcgtagat gacgatgccc
    16621 tgctgaccga actgctgacc gaatacctga gcgccgaagg tctgaacgtc cgcagcgttc
    16681 ccgacgggga agcaggcgta caggaaatcc tgagcgggca gtacgatgta gtcgtattgg
    16741 attccatgat gcccaaaatg aacggcttgg atgtcttgaa aaacgtacgc gcccgaagca
    16801 ccgtccccat catcatgctg accgccaaag gcgacgacat cgaccgaatc atcggcttgg
    16861 aaatgggcgc ggacgactat gtccccaaac cctgcacacc acgcgaactc ttggcacgca
    16921 tcaatgccat cctgcgccgc gcacaacaca gcggcgaaca gaacaacgca cccaacagca
    16981 tctccgtcag cgatgtcgtc ctgtaccccg ccaaacgcca ggcatccgtc aaagacatgc
    17041 cgctcgaact gaccagcacc gaattcaacc tgctcgaagt cctgatgcgc catgccggac
    17101 aggtagtcag caaagaaacc ctgtccgtcg aagcactcga ccgcaagctg gcaaaattcg
    17161 accgcagtat cgacgtacac atctccagca tccgccacaa gttgggcgat gcctctctga
    17221 ttcaaaccgt acgcggcttg ggctacctgt ttgtcaaaaa ctgaagcaac gggcaaatga
    17281 aactgttcca acgcattttc gccacatttt gcgcagttat cgtctgtgca atctttgtgg
    17341 cgagtttttc tttttggctg gtgcagaaca cccttgccga aaaccagttc aaccaacgcc
    17401 gcaccatcga aaccactttg atgggcagca tcatttccgc attccgggca cgcggggacg
    17461 cgggcgcgcg cgaaatcctg acggaatgga aagacagccc cgtctcatcg ggcgtgtacg
    17521 ttatacaggg cgacgagaaa aaagatatcc tgaaccggta tatcgacagc tacaccatcg
    17581 aacgcgcccg gcttttcgcc gccggacacc cgcattccaa cctcgtccat atcgaatacg
    17641 accgcttcgg cgaagaatac ctgttcttca ccaaagactg ggacaaactc caagcccgcc
    17701 gcctgcccag ccccctgttg atccccggtc tgccgctcgc cccgatttgg cacgaactca
    17761 tcatattgtc cttcatcatc gtcgtcggac tgctgatggc gtacatcctc gccggcaata
    17821 tcgccaaacc catcagaatc ttaggcaacg gcatggacag ggtggcaaac ggagaacttg
    17881 aaacccgtat ctcccaacag gtcgacgacc gcgacgacga attgtcccat cttgccatcc
    17941 aattcgacaa aatggtggaa aaacttgaaa aactcgttgc caaagaacgc cacctgctcc
    18001 atcacgtctc ccatgaaatg cgttctcccc ttgcgcgcat gcaggcaatt gtcggactga
    18061 ttcaggcgca gccccaaaaa caggagcaat atctcaaacg gctggaaggc gaactgaccc
    18121 gcatggatac gctggccggg gaactgttaa ccctgtcccg tctcgaaact tccaatatgg
    18181 ctttggaaaa agaaagcctg aaactcctgc ccttcctggg caacctggta gaagacaatc
    18241 aaagcattgc ccagaaaaac ggacaaacgg ttaccctgtc tgccgacgga aaaatccccg
    18301 aaaatacaac catccttgcc aacgaaagct acctgtaccg cgccttcgac aacgtcatcc
    18361 gcaacgccgt caactacagt cccgaaggca gcaccatcct gatcaacatc ggacaagacc
    18421 acaaacactg gataatcgac gttaccgaca acggccccgg cgtggacgaa atgcagctcc
    18481 cgcacatctt caccgctttc taccgtgcag actccagtgc gaacaaaccc ggaacaggac
    18541 tggggcttgc attgacccaa catattattg aacagcactg cggaaaaatc atcgccgaaa
    18601 acatcaaacc gaacggtctg cggatgcgct tcatcctgcc caagaaaaaa accggttcca
    18661 aaacagaaaa aagtgcgaac tgaccataat gccgtctgaa ccggcttcag acggcattgc
    18721 acaaacagtt accccaaaac aaacacacag cgggtgcatc tccaacaatc acgcacatac
    18781 cgtccgcaaa ggaatatatc atgtcggcac aaaccgatcc gggctacttt ttcatgccca
    18841 accacattat tctgataggc gcgagcgaac gtccgtacag cctgggcgaa cgcgtactca
    18901 gcaacctgct gagtacgccc tttcaaggaa aaatcacccc cgtaaacccg cgccaccaca
    18961 ccatagccgg gcttcccgcc tacaccagcc tcaacaaaat ccccggcaat gcagacctga
    19021 ttattgccgt taccctaccc gacagttacg acaccctctt caaaacctgc cgcaaaaagc
    19081 agctccgaca catcatcctc atacaggact gggacagcct gtctgccgca gaactgcaca
    19141 ccgccgaaac tgccatccgc aaacaccacg gcaacggact taacatcacc gcctgcacca
    19201 ccgcaggcat ccaactgccc tcactcggac tcaacatcag tacccaagac ggatacgccg
    19261 caggccatac cgccatactg accggcaatg ccgccgtcag ccgtcaaatc gacaacatcc
    19321 tgaacaaact ccgtcaaggc acatctcgcc acatcagcct gcatcccggc atcagcccca
    19381 tcacatccgc cgattggctc aaccgcttcg gacacagcct gcacaccaaa accgccgtcc
    19441 tacaccacaa ccctgaagaa gaccagcgca aactgttcag cgcaatccgc caatttaccc
    19501 gccatacgcc gctgattctc cacatcacct gccgcacgac agaaaccgac cgtgccgtac
    19561 tgcacagcct cgcccgacac tgcaacttcc tcatcagttt caacgccgac gacctcgaag
    19621 ccgcactgcg cgcccaactg tccgaccttc ctcccctgtc ccgactcgac atcctgtccg
    19681 acacgcctgc cgaatggctg aacgcgcacg cgccaaaaac cctcgccctc cactttccca
    19741 accttcccca ccacatccgc aacggacacc tgaccggcac acccacaccc tcaatctgcc
    19801 acgacatcgc ctcacgtcag cttgcccgcc ccgacaccca agccgtcctg accatcctca
    19861 gcccctccgg acacgaggat tacaaaaaaa cagtacgcgc ccttatccgc ctgtccgaac
    19921 agaccgccaa acccctgctc gtcagcagcc ccttttcaga cggcataacc catttcgaca
    19981 cccccactca ggcaatccgc accctttcct accgcaacac cgccgccgcc ctgaaacagg
    20041 cacagctcga cattgcaccg ccgcagccat gccgtctgaa aacaccgcaa ccccaaaaca
    20101 tcaaaaaagc cctcgaagcc gccgaccccg ccctaatcgc cgaagccctg cacctccccc
    20161 cctaccggca caccacccat aacgccgtcc aattccaatt cggcagccac tccctctatg
    20221 gcgacatcct gaccgcacgt tgcaacggac aaaccactgc cgtactcccg ccgtttacca
    20281 cgctcgacag ccgccacctt gcccgctttg ccgaactcga cggcacacaa accctcgacc
    20341 agttcctgca cacactgacc atcattcccg aataccgcca acacattctc ggcatcaccc
    20401 tcaacctaaa cggcggacaa tacagcagcg atttcatctt aaagacaccc gaaacacacg
    20461 acacgcccaa acgcaagaac acaggcaaag ccgcccaaac cctcgaacat gccgccgcaa
    20521 aaatgcagag tgccgccgca tacctgaaac acaaaaaccc gacagccgcc gaatttctcc
    20581 gccacacaag cgaagccgcc gcagaactgc tcggcagcaa aaccgaaacc ggagcagccg
    20641 tacccaacgt acttgccccc tatcccgcag cataccccaa aacactgtcc ctaagaaaca
    20701 acacgaccgt taccattatc ccccttttgc ccgaagacgc agaagccaaa cagcagttcg
    20761 tccgcagcct cggtcccgaa gcacggtaca cacgcttcat gacccacacc aacgaactgc
    20821 ccacagccac gttggcacgc ctgtgcaacc ccgactacca ctgtgaagcc gcatggacgg
    20881 caagggatgc cgacagcaac atcgtcgccg tcgtccgcca cagccgcctg aaccgcaacg
    20941 aatgcgaatt cggcatcaca ctggcggaac atatgcgcgg cagcgggctg gcacagaaaa
    21001 tgatggaact catcatccaa accgccgcac agcaaggcta ccggactatg agcgccgaca
    21061 ttctcaaaac caataccccc atgatcaaac ttgctgaaaa atcaggattt accctcaagg
    21121 aatcggacac cgaaaaaaac ctgtaccgcg catatctgaa ccttgcggca gacaaaacaa
    21181 cagaaaaaac aaataaaaac ttgcacaccg accacaaaat aacctaaaat cggcagtttc
    21241 catatatccg attttctcaa aaggactcaa aatggtagtt atccgtttgg cacgcggcgg
    21301 ctcgaaacac cgccctttct acaacgtcat cgttactgac tcacgcagcc gccgcgacgg
    21361 ccgcttcatc gaacgcgtag gcttctacaa ccccgtagcc aacgaaaaac aagagcgcgt
    21421 ccgcctcaac gcagaccgcc tgaaccactg gattgcacaa ggcgcgcaag tcagcgactc
    21481 cgttgcaaaa ctgattaaag aacaaaaagc cgcctaatcc gcgtttgccg ccatgacaga
    21541 cactcaaaac cgggtagcca tgggctacat caaaggcgta ttcggcataa aaggctggtt
    21601 gaaaattgcc gccaacaccg aatattccga cagccttttg gactaccccg agtggcattt
    21661 ggtcaaggac ggcaaaaccg tcagcgttac ccttgaagcc ggaaaagtcg tcaacggcga
    21721 actccaagtc aaattcgaag gcataaacga ccgcgacttg gcattctcat tgcgcggtta
    21781 caccatcgaa ataccccgtg aagcattcgc cccgacagaa gaagacgaat actactggac
    21841 agacttggtc ggcatgaccg tcgtcaacaa agaccatacc gttttaggca aggtaagcaa
    21901 cctgatggaa accggcgcaa acgacgtatt gatgattgac ggagaacacg ggcagattct
    21961 gattccgttc gtttcccaat atatcgaaac cgtcgatacc ggcagcaaga ttattactgc
    22021 cgactggggt ttggactact gatgcttatc caggcagtta ccattttccc cgaaatgttc
    22081 gacagcatta cccgctacgg cgtaacggga cgcgcgaaca gacagggaat ctggcagttt
    22141 gaagcagtca atccccgaaa gtttgccgac aacagattgg gctacatcga cgaccgcccg
    22201 ttcggcggcg gtccgggaat gattatgatg gctccgccgc ttcatgcggc aatagaacac
    22261 gccaaagcac aatcttccca agctgcaaaa gtcatttacc tcagccccca aggaaaaccg
    22321 ctgacacacc caaaagcggc agaactggca gaacttccgc atctgattct gctgtgcgga
    22381 cgctatgagg gaatagacga aagactgctg caaaccagcg tcgatgaaga aatcagcatc
    22441 ggagacttcg ttgtttccgg cggagagctt cccgccatga tgctgatgga tgcggtattg
    22501 aggctcgtac ccggcgtatt gggcgatatg cagtctgccg aacaagattc gttctcaagc
    22561 ggcattttgg actgccccca ctacaccaaa cccttagaat ttcaaggcat ggctgttccg
    22621 gaagtattac gttccggcaa tcatggcttg atagcggaat ggcggttgga acaatcgctg
    22681 cgccgcacct tggagcgcag acccgatctt ttggaaaagc gcgttttaat cccaaaggaa
    22741 tcccgcctct tagaaaccat ccggcaagag caacgggaaa tccaatcata atttaggaaa
    22801 aaaaacaatg aacctgattc aacagctcga gcaagaagaa attgcccgcc tgaataaaga
    22861 aatccccgaa ttcgcaccgg gcgacaccgt agtcgtatcc gtacgcgtcg tggaaggtac
    22921 ccgcagccgt ctgcaagcct acgaaggcgt tgtcattgca cgccgcaacc gtggtctgaa
    22981 cagcaacttc atcgtccgca aaatctccag cggcgaaggt gtggaacgta ctttccaact
    23041 gtactctccg accgtcgaaa aaatcgaagt caaacgccgt ggcgacgtac gccgtgccaa
    23101 actgtactac ctgcgcggcc tgaccggtaa agctgcacgc attaaagaaa aactgcctgc
    23161 acgcaaaggt tgattcaaac cgttttcccc caatgccgtc tgaaccttca gacggcattt
    23221 tttatcgccg caatatcaac aagacaacga tattcccaat ccgcctcccc tgcttaacaa
    23281 gcactcccgt ctattcaaac aaaatttaca gaccaaacgt tatacagtat catagccgcc
    23341 atataacgat aaccgctttg ccaacttccc tttcaaaacc gcaaatcatt atgacacctt
    23401 ccctcttact gtcaggattg accttccgac tcttcctcgc cctgattgcc gtatcccttt
    23461 tatggggcgt ttacctttgg gcggtatcgg tatgagcatc atcgttgaaa acctgaccgt
    23521 cagctaccgc cgccgacctg ccgtgcacca tgtggacatt acttttgaag aacatagtat
    23581 gtgggcggtt ttcggtccca acggcgcggg gaaatccacc tttctcaaat ccttaatggg
    23641 attgcagcct atcgatacgg gcagcatccg gctggacgga ttgacccgtc agaacattgc
    23701 ctaccttccc cagcagtccg acatcgaccg ctcgcagcct atgaccgttt tcgacttggc
    23761 ggcaatgggg ctatggtatg aaatcggttt tttcagagga ataaatgccg ttcaaaaaca
    23821 acgcgttcac gaatctttgg aacgcgtcgg gatgcggcag tttgcacgcc gccaaatcgc
    23881 ccacctctca aacggacaat tccaacgcgt cctttttgcc cgaatgctgg ttcaaaatgc
    23941 aaaattcctg cttttggacg aacctttcaa cgccgttgat gcacggacga cctacgaact
    24001 tctcgacgta ttgcagaaat gccattgcgg cggacacgct atcattgccg tactgcacga
    24061 ttacgaacaa gtccgtgcct actttcccag taccctgctg ctcgcccgag aaaaaattgc
    24121 ggcaggcgca accgagacca ttctgacaga accctacctc gcccaagcca acgccaaaat
    24181 gcagcaacag gaaagccccg actggtgcgc ctcataaata ctgcccgaaa ccacgcccca
    24241 caccagacac catgaatctc tacgacctgc tccttgcccc ctttgcagaa ttcgacttta
    24301 tgcgctacgc cctcgcatcc gtcttctgcc tgtccctcag tgccgcaccc gtcggcgtat
    24361 tcctcgtcat gcgccgtatg agcctgatag gcgacgcatt gagccacgcc gtcctgcccg
    24421 gtgccgccgt cggctacatg tttgccggct tgagcctgcc cgccatgggt ttgggcggcg
    24481 tagccgcagg catgctgatg gcactgcttg ccggactcgt cagccgcttc accaccctga
    24541 aagaagatgc caactttgct gccttttatc tcagcagcct cgccatcggc gtagtcctcg
    24601 tcagcaaaaa cgggagcagc gtcgatttgc tccacctcct tttcggctct gtacttgccg
    24661 tcgatattcc tgccctgcag ctcatcgccg ccgtctccag cctcacgctc attacccttg
    24721 ccgtcatcta ccgcccgctc gtactcgaaa gcatcgaccc cctgtttctc aaatccgtcg
    24781 gcggcaaagg cgggctttgg cacgtcctct ttctcgtcct ggtcgtcatg aacctcgtat
    24841 ccggctttca agccctcggc acactcatgt ccgtcggact catgatgctg ccagccatta
    24901 ccgcccgcct gtgggcgaag cacatgggcg cactcatcct cctatccgtt ctgacagccc
    24961 tgctgtgcgg cttgagcgga ctgctcattt cctaccacat cgaaatccct tccggtcccg
    25021 ccatcatcct ctgttgcagc gtcctttatc tcttttccgt catactcggc aaagaaggcg
    25081 gcattctgac caaatggctc aaaaaccacc gccaccacac cacctgaccc cgcaagccgc
    25141 ttgaacaaac aggcagcttg ttttaccata acagatgtta cgttatatca caggagaaca
    25201 gttatgaaac acctcaaact cacccttatt gccgcattgc taaccaccgc cgcaactgcc
    25261 gcccccctgc ccgtcgtaac cagcttcagc attttaggcg acgttgccaa gcaaatcggc
    25321 ggagagcgcg tatccataca aagtttggtc ggagccaacc aagatactca tgcctatcac
    25381 atgaccagta gcgacattaa aaaaatccgc agtgcaaaac tcgtcctgct caacggctta
    25441 ggacttgaag ctgccgatgt acaacgtgcc gtcaaacaga gcaaagtatc ctatgccgaa
    25501 gcgaccaaag gcatccaacc cctcaaagcc gaagaagaag gcggacacca tcatgaccac
    25561 gaccatgacc acgaaggaca ccaccatgac cacggcgaat ttgacccgca cgtctggaac
    25621 gaccccgtcc ttatgtccgc ctatgcccaa aacgtcgccg aagccctgat aaaggccgac
    25681 cccgaaggca aagtttatta tcaacaacgc ttgggcaact accaaatgca gcttaaaaaa
    25741 ctgcacagcg acgcacaagc cgcatttaac gccgtccctg ccgccaaacg caaagtcctg
    25801 accgggcacg atgccttttc ctacatgggc aaacgttacc atattgaatt catcgccccg
    25861 caaggtgtga gcagcgaagc cgagccttca gccaaacaag tcgccgccat catccgacaa
    25921 atcaaacgcg aaggcatcaa agccgtcttt accgaaaaca tcaaggacac ccgtatggtt
    25981 gaccgtatcg ccaaagaaac cggtgtcaac gtcagcggca aactgtattc cgacgcactc
    26041 ggcaacgcgc ccgcagacac ctacatcgga atgtaccgcc acaacatcaa agccttgacc
    26101 aacgcgatga agcaataaaa ccgtttgatt ggtttgaatt tgaaaaggca aaaggccgtc
    26161 tgaaaattat agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacag
    26221 atagtac
//
LOCUS       DAWFJR010000022        23579 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWFJR010000022 DAWFJR010000000
VERSION     DAWFJR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23579
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.022"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(369..1409)
                     /gene="queA"
                     /locus_tag="WGR04_000817"
     CDS             complement(369..1409)
                     /gene="queA"
                     /locus_tag="WGR04_000817"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ0794406.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVTVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVADRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGWNEGVVR"
     gene            1526..1783
                     /locus_tag="WGR04_000818"
     CDS             1526..1783
                     /locus_tag="WGR04_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ0794407.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPKHRQPLKEQKWICAN"
     gene            1765..2220
                     /locus_tag="WGR04_000819"
     CDS             1765..2220
                     /locus_tag="WGR04_000819"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ0794408.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKVGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            2332..3693
                     /gene="accC"
                     /locus_tag="WGR04_000820"
     CDS             2332..3693
                     /gene="accC"
                     /locus_tag="WGR04_000820"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ0794409.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAIYLAERDCSMQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            3811..4698
                     /gene="prmA"
                     /locus_tag="WGR04_000821"
     CDS             3811..4698
                     /gene="prmA"
                     /locus_tag="WGR04_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ0794410.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTENEQA
                     IFGEPGMPTEQIWQQSKVIALFGEHDEAAAIIQTAAQECGLKDLAYTGETLEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEVPEGSAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGRDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEEGWARLSGTKR"
     gene            4716..5279
                     /gene="orn"
                     /locus_tag="WGR04_000822"
     CDS             4716..5279
                     /gene="orn"
                     /locus_tag="WGR04_000822"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ0794411.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDELLDNMDEWNTATHGRTGLTQRVRESLHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            5290..5560
                     /locus_tag="WGR04_000823"
                     /pseudo
     CDS             5290..5560
                     /locus_tag="WGR04_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
     gene            complement(5776..7059)
                     /gene="hemL"
                     /locus_tag="WGR04_000824"
     CDS             complement(5776..7059)
                     /gene="hemL"
                     /locus_tag="WGR04_000824"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ0794412.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVIEAMREAALGGLSFGAPTEGEIVIAEEIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FRTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIEGFKQFFHGMLDRNVAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAE"
     gene            complement(7123..7296)
                     /locus_tag="WGR04_000825"
     CDS             complement(7123..7296)
                     /locus_tag="WGR04_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ0794413.1"
                     /translation="MKSERRHWEWCVDYDWEGVVRDYRFTHKVEKDERSVIRDAVGVI
                     RKEAGKSLSQPEK"
     gene            complement(7609..7689)
                     /locus_tag="WGR04_000826"
                     /pseudo
     CDS             complement(7609..7689)
                     /locus_tag="WGR04_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
     gene            7892..9220
                     /gene="miaB"
                     /locus_tag="WGR04_000827"
     CDS             7892..9220
                     /gene="miaB"
                     /locus_tag="WGR04_000827"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ0794414.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(9305..11218)
                     /gene="dxs"
                     /locus_tag="WGR04_000828"
     CDS             complement(9305..11218)
                     /gene="dxs"
                     /locus_tag="WGR04_000828"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ0794415.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRTFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLEDLR
                     GRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKESAAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGEKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLNDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(11303..12220)
                     /gene="xerC"
                     /locus_tag="WGR04_000829"
     CDS             complement(11303..12220)
                     /gene="xerC"
                     /locus_tag="WGR04_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ0794416.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDEGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            12414..13478
                     /locus_tag="WGR04_000830"
     CDS             12414..13478
                     /locus_tag="WGR04_000830"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ0794417.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMANRYAKGELNQIVK"
     gene            13498..14457
                     /locus_tag="WGR04_000831"
     CDS             13498..14457
                     /locus_tag="WGR04_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HEZ0794418.1"
                     /translation="MPSEPLFGRHLIFASLTCLIDAVCKKRYHNQNVYILSILRMTRS
                     KPVNRTAFCCLSLTAALILTACSSGGGGVAADIGAGLADALTAPLDHKDKSLQSLTLD
                     QSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKISRFDFIRQIEVDGRLITLESGE
                     FQIYKQDHSAVVALQIEKINNPDKIDSLINQRSFLVSGLGGEHTAFNQLPDGKAEYHG
                     KAFSSDDAGGKLTYTIDFAAKQGHGKIEHLKTPEQNVELAAAELKADEKSHAVILGDT
                     RYGSEEKGTYHLALFGDRAQEIAGSATVKIREKVHEIGIAGKQ"
     gene            14596..15273
                     /gene="tsaB"
                     /locus_tag="WGR04_000832"
     CDS             14596..15273
                     /gene="tsaB"
                     /locus_tag="WGR04_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ0794419.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGETLLFHQEAGSRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASMPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEETPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHAELLYVRNKIALTAKEQ
                     AERKART"
     gene            15270..15710
                     /gene="rimI"
                     /locus_tag="WGR04_000833"
     CDS             15270..15710
                     /gene="rimI"
                     /locus_tag="WGR04_000833"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ0794420.1"
                     /translation="MNIRRASPSDCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWFAHLPEDTQRLLLE
                     VRAGNTAAQALYAAHGFTDAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            15704..16438
                     /locus_tag="WGR04_000834"
     CDS             15704..16438
                     /locus_tag="WGR04_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ0794421.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVPTRKPAPETETPLPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGVLLDNILKAVGLDAAYVHKTCWVKTAAVGNPMP
                     SEQAVADALGQIARELDSCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARTWQVLKQLKHALQAGGS"
     gene            16500..17141
                     /gene="pyrE"
                     /locus_tag="WGR04_000835"
     CDS             16500..17141
                     /gene="pyrE"
                     /locus_tag="WGR04_000835"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ0794422.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRQSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            17215..17649
                     /locus_tag="WGR04_000836"
     CDS             17215..17649
                     /locus_tag="WGR04_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794423.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            17646..18956
                     /gene="argA"
                     /locus_tag="WGR04_000837"
     CDS             17646..18956
                     /gene="argA"
                     /locus_tag="WGR04_000837"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ0794424.1"
                     /translation="MSAPDLFVAHFREAAPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDHHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFYLDMLQTAASAAVSLQAEKLVYLTLSDGIARPDGTL
                     AETLSAQEAQSLAEHAGSETQRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKVYRSNGRNSHILVRRLHR"
     gene            19052..21067
                     /locus_tag="WGR04_000838"
     CDS             19052..21067
                     /locus_tag="WGR04_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ0794425.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARELS
                     DGILAQLQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLSKSGGDTAYTLEVDLEARELVKGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIGEDGMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSAEGEAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGEIGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFTDGKWQEAELPRLPSGALEMTDQPWGGDVVYLAAGDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFVSDGINVRQFWTASADGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGVPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGMSSPRHIGLQGGSNGGLITAAAFVCEPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRWLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLHETSAQSWLYSPDGGGHTGNGTQRESADELACVLLFLKEFLG"
     gene            complement(21181..22137)
                     /locus_tag="WGR04_000839"
     CDS             complement(21181..22137)
                     /locus_tag="WGR04_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0794426.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            22373..23338
                     /locus_tag="WGR04_000840"
     CDS             22373..23338
                     /locus_tag="WGR04_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0794427.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGARQLIQAAEQLKAAFEKAEPVAAGKE"
BASE COUNT         5379 a         7120 c         6312 g         4768 t
ORIGIN      
        1 aatttatctt ataaacaaac aaaggcagcc tgaaagattg aatggtctgc acccctaagg
       61 ttggactaac caaccgacta aggtgcagat tattttttgt tgctttttca gcttttcgtt
      121 gggttagata ttcttgccca ctgttttcag gcagccttga atacaaaaaa atggcgtatg
      181 taatatgttt atacgaccaa aacggaatga attttaacgt attgcgcgtc atcaacaatg
      241 actgagtttc tcgcctctcg cgcctgaatc tatgtcatac agttcctttc attcaaatca
      301 acaaaagaat gccgtccgaa cgtccgttca gacggcactt gtcttccccc aatagacttg
      361 gggctgttct aacgtaccac cccttcgttc caccccaaaa ccatcgcatc gccgtagctg
      421 aagaaacggt attcgcgttc gaccgcataa cgatacgcgg cgcggatatg acccataccc
      481 gaaaacgcgc tgacaagcat cagcagcgtc gatttcggca gatggaaatt ggtaaccagc
      541 ctgtcggcaa cattaaaacg gtagcccggc gtgatgaaaa tatcggtgtc gccctgcccc
      601 gctttcagac gacccgtcgc acgcgcggca gactcgaggg cgcgcatgga agtcgtgccg
      661 accgcccaga ctttgttccc ccgggctttt gccgcctcaa cggcggcggc ggtttcagac
      721 ggcacttcaa accattcgct gtgcattttg tgttcttcga ttttatcgac gcgcacgggt
      781 tggaacgttc ccgcaccgac gtgtagggtt acttctgcgg ttactgcgcc tttgtctttc
      841 aggcggcgca aaagttcttc cgtaaaatgc agacccgccg tcggcgcggc gaccgcgccc
      901 tgatatttgg cataaacggt ttgataacgg ctgtcgtcat ccgcatcggc ggcgcgttcg
      961 atataaggcg gcaggggcag gtgtccgttc tgttccaaaa gttcgtaaac ggtttgaccg
     1021 ccttcaaaac gcaggcagaa cagttcgccc tcacgcccga ccgtcacggc gcggatgccg
     1081 ccttcaaaca ccagccccat accgggcttg ggcgatttgg acgaacggat gtgcgccagt
     1141 gcggtatggt tgtccaacac gcgctcaatc agggcttcga tcctgccgcc gctgtctttc
     1201 tgcccaaaca gccgcgcctt catgactttg gtgttgttga acaccaaaac gtcgcccgcc
     1261 tcaacataat ccggcaaatc gccgaacacc cggtcttgca gcggcatatc gggcaacgca
     1321 accaaaaggc ggctgctgcc gcgcacttcg ggcggatgct gggcaatcag cttttcgggc
     1381 agggtaaaat caaaatctga aatatccatt tttacactct cgttcgggca agccgccatt
     1441 atacgcactt tagccctttt tcagacggca tctttgtccg aaaaaccaac agattagaat
     1501 aaacactctt aacccggggc atcttgtgcg caaaatcaaa cttcctgcac atttccccca
     1561 aaaaccgccg ttttttgata ttttactgga catttaccga caacttcggg aaaataaaca
     1621 cattctcacg gtcgttttcc accacaggaa aaccgtatcc gaacaccatt ccgcccggtt
     1681 tgcgccgttg ccgcaagccg gctgttttct gaaaaaccaa cgcaacaacc cgccgaaaca
     1741 ccggcagcct ttaaaggaac agaaatggat ttgcgcaaat taaaaaaact gattgatttg
     1801 gttgaagaat cgggtatcgc cgaaatcgaa gtaaccgaag gcgaggaaaa agtccgcatc
     1861 acccgaacca tcgccgccgc acccgtttac gccgcgcccg tacctgccgc cgcgccggcc
     1921 gtaacgcctg ccgccgcacc cgttgcggca tccgcgcctg ccgccgcacc tgccgcccgc
     1981 gatttgtccg acgcgcaaaa atcacctatg gtcggcacgt tctaccgcgc acccggcccg
     2041 aatgccgcgc cttttgtcga agtcggccaa caggttaaag tcggcgacac gctgtgcatc
     2101 atcgaagcga tgaagctgat gaacgaaatc gaagccgaaa aatccggcac ggtcaaagaa
     2161 attttggtcg aaaacggtac gcccgtcgaa ttcggcgaac cgctcttcat catcggataa
     2221 tcctgttttc agacggcata aacttccgat gccgtctgaa atgctttccc ccttcagcgt
     2281 tcccgcaccc ttttttacgg acgggttgcc ggaaccgcag gaaaggtcat catgctgaaa
     2341 aaagttttaa tcgccaaccg aggcgaaatc gcattacgcg tactccgtgc ctgccgcgaa
     2401 atgggcattg ccaccgtcgc cgtgcattcc gaggccgaca aagacagcct gcacgtcaaa
     2461 ctcgccgacg aatccgtgtg catcggccct gccgcttccg cgcaaagcta ccttaacgtc
     2521 cccgccatta tcgccgccgc cgaagtaagc tgcgcggacg ctgtccaccc gggctacggt
     2581 ttccttgccg aaaacgccga tttcgccgaa caggtcgagc agtccggctt tacctttatc
     2641 ggcccgaaac ccgacaccat ccgcctgatg ggcgacaaag tctccgccaa acacgcgatg
     2701 atagcggcag gcgtaccctg cgtccccggt tctgacggcg cattgccgga cgacggcgaa
     2761 gaaatcctca aaatcgccga taaagtcggt tatcccgtca ttatcaaagc ctccggtggc
     2821 ggcggcggtc gcggtatgcg cgttgtcgag aaaaaagaag acctcctcca gtctgtcgaa
     2881 atgaccaaag ccgaagcagg cgcggcattc ggcaacccga tggtttacat ggagcgctac
     2941 ctgcagcgtc cgcgccacgt cgaaatccaa gtgattgccg acgaacacgg caacgccatc
     3001 tacctcgccg agcgcgactg ctccatgcag cgccgccacc aaaaagtcat cgaggaagca
     3061 ccggctccgt tcatcactga aaaagaacgc gccaaaatcg gcaacgcctg tgccgacgcc
     3121 tgcaaacgca tcggctatcg tggcgcaggt acgtttgaat tcctgtacga agacggcgaa
     3181 tttttcttta tcgagatgaa tacgcgcgtt caggttgagc atccggttac cgagctgatt
     3241 accggcgtgg acatcgtgca ggagcaactc cgcatcgccg ccggcctgcc tttgcaatac
     3301 aaacaaaagg atattcaagt cgaaggccac gcgtttgagt gccgtatcaa cgccgaagac
     3361 ccgtacaact tcatccccag cccgggcccg attgaaagct gccacctgcc cggtggcttc
     3421 ggtatccgcg tggacagcca catttaccaa ggctaccgca tcccaccgta ctacgacagc
     3481 ctgatcggca aaatctgcgt acacggcaaa acgcgtgaac aggcaatggc gaaaatgcgc
     3541 gtcgcactcg ccgagctggc ggtaaccggc atcaaaacca atacgccgct tcaccgcgac
     3601 ctgttcgccg atgcgggttt ccaaaaaggc ggcgtcagca tccactattt ggaacactgg
     3661 ctggaagatc gcaaagccaa acaggacaaa taagccgtcc ccgatatgcc gtctgaaaca
     3721 gtttgtccgt tttcagacgg catttccgtt acacacccat ccccttatac agtacgaccc
     3781 tattccgaag gtctgttaag aaagcccgct atgccctacc aacaaatcac cgtcaacgtc
     3841 aacgatgccg tcgccgaacg cctcgccgac gcgctgatgg aacacggcgc actctccgcc
     3901 gccatcgaag atgcctacgc cggcaccgaa aacgaacaag ccatctttgg cgaacccggt
     3961 atgccgaccg aacaaatctg gcagcagagc aaagtcatcg ccctgttcgg cgaacacgac
     4021 gaagccgccg ccatcattca aaccgccgca caagaatgcg gattaaaaga cttggcatac
     4081 accggtgaaa ccctcgaaga ccaagactgg gtacgcctca cgcaatcgca attcgacccc
     4141 atccggattt ccgaccgcct gtggattacc ccctcttggc acgaagtccc cgaaggcagc
     4201 gccgtcaacc tacgcctcga tcccggactc gccttcggca ccggcagcca cccgaccacg
     4261 cgcctctgcc tcaaatggtt ggatacacaa ctcaaaaacg gcgaaagcgt cctcgactac
     4321 ggctgcggtt cgggcatcct gaccatcgcc gccctcaaac tcggcgcagg ttctgccgtc
     4381 ggcgtcgata ttgacgaaca agccgtccgc gccggcaggg acaacgccga acagaacaac
     4441 gtcgatgcac aattcttcct gcccgacggt ctgcctcaag ggcaattcga cgtagttgtc
     4501 gccaacatcc tcgccaaccc tttgcgtatg cttggcgaaa tgctcgccgc ccgcaccaaa
     4561 cagggcggac gcatcgtgtt gtccggtttg ttggacgaac aggccgaaga actcggcggc
     4621 atttacagcc aatggttcga cctcgacccg gcggaaaccg aagaaggctg ggcgcgcttg
     4681 agcgggacaa aacgctgaaa cggaaaggaa acaccgtgca ggacaaaaac aacctctgct
     4741 ggctcgatat ggaaatgacg gggcttaatc ccgaaaccga ccgcattatc gaagtcgcga
     4801 tgattattac cgactcggat ttgaatgtat tggcgcaatc cgaagtttac gccgtccacc
     4861 aaagcgacga gctgctcgac aatatggacg aatggaacac cgccacacac ggcaggacgg
     4921 ggctgacaca gcgcgtgcgc gaatcgttgc ataccgaagc cgaagtcgaa cagaaactgc
     4981 tggactttat gtcggaatgg gtacccggac gcgccacgcc gatgtgcggc aactccatcc
     5041 accaagaccg gcgttttatg gtcaaatata tgccgaaact ggaaaactac ttccactacc
     5101 gcaacctcga cgtttccacg ctgaaagaac tcgccaaacg ctggaatccg cccgttgcca
     5161 aaagcgtcgt caaacgcggt tcgcacaagg cattggacga cattttggag agcatcgaag
     5221 aaatgcgcca ctaccgcgaa cactttctga tttccgcccc gagagccgaa gcgcaataag
     5281 aaacaaacaa tgccgtctga aacgctgttt gcatttcaga cggcattttt acagcagatt
     5341 gaaatcaaaa atatacacgc ccgtcattcc cgcgcaggcg ggaatccgga aggtcgggct
     5401 tgttggtcat gaagtggtct tgaaaatgaa acgttatcga aaatctgacg aactgaaaga
     5461 aaaatatagc gatatagatg agacttttga atttataaag gggtttatct atgtaattgc
     5521 ccttgctttt accgcatatg tcgctatccg ttttttctga tgcgcttccg ataaaatgcc
     5581 gtctgaaggc ttcagacggc attgtttcag gtggcggcgg cttcctgccg ggcgcggctg
     5641 atttcggccc gttattttca atcattacag aaactgaaag gtctggattc ccgcctgcgc
     5701 gggaatgacg gcggtgtgca ttcttacttc aatctgccaa acagatcgaa cagagaaacc
     5761 ctgtccgtca aaacatcatt cagccatcgc cttgaacact tcaaccgcaa ccgcaaccgt
     5821 ttcgtcaatc agctcgggcg tatgcgcggc ggaaacgaaa cctgcttcat aagcggacgg
     5881 gccgaaggcg acattgcggt cgagcatccc gtggaaaaac tgtttgaagc cttcaatatt
     5941 ggaacgcgcc atatcggcat aatttcgcgg cgcgtgtgcg gcgaaataca gaccgaacat
     6001 accgcccacg ctgtcggcgg tgaactcgat gcccgccgca tccgctgccg tccgaaaacc
     6061 ttgaaccaac tgttcggtac gcgccgtcag gttttcatag aagccttcgc gccggatgat
     6121 ttccagcgtt ttcaagccgg cggcgacggc aatcgggttg cctgataatg tgcctgcctg
     6181 atacacgccg cccagcgggg aaatacattc cataatgtct ttgcgtccgc cgaacgcggc
     6241 aagcggcata ccgccgccga tgactttgcc catcgtggtc aggtcgggcg tgatgccgtg
     6301 cagcgattgc gcgccgccga gcgcgacgcg aaaaccggtc atcacttcgt cgtaaatcaa
     6361 cacggcgccg tgtttttcag tcaattcgcg cagggctttg acgaaggctt cggtcgggcg
     6421 gacaaggttc atattgccga caaaaggctc caaaatcaca caggcaatgt cattgccgct
     6481 ttgagcaaag gcttcttcca attgggcgat gttgttgtac tcaaggacta aagtgtgttt
     6541 ggtaaagtcg gcaggcacac cggcggaaga gggattaccg aaggtgagca gaccgctgcc
     6601 tgctttcacc aacaggctgt cggaatggcc gtggtagcag ccttcaaatt tgatgatttt
     6661 gtcacggccg gtaaagccgc gtgccagacg gatggcggtc atggtggctt cggtaccgga
     6721 gctgacaagg cgcagccgtt cgacggacgg catgattttg gcgatttctt cggcaatgac
     6781 gatttcgcct tcggtaggcg cgccgaacga caaaccgccc aaggcagctt cgcgcatggc
     6841 ttcgatgact tcggggtgcg cgtgtccgac aatcgcgggc ccccacgaac cgacatagtc
     6901 ggtgtagcgc gtgccgtttt cgtcccaaac atacgcgcct tcggcttttt tgatgaagcg
     6961 cggcacgccg ccgacgctgc cgaatgcgcg gacgggggaa ttcacgccgc cggggatgat
     7021 ggctttggcg cggtcgaata aaatttcgtt acggttcata tttatcctca aatgccgtct
     7081 gaacggcagg tttcgggctt agaagcagaa agtcccattt tatcattttt caggttgcga
     7141 caaggatttg cctgcttctt tgcggatgac gccaaccgca tcccggatga cggaacgctc
     7201 gtctttttcc actttatgtg taaagcggta gtcccggaca actccttccc agtcgtaatc
     7261 cacacaccac tcccaatgcc ggcgttctga tttcatataa atgaaattgg tcggcaaaaa
     7321 attataaatc ggcaggctga cttcatgata ggcataacaa ccgaaagggt tgcgcttccc
     7381 gaaacgtgcc tctacctccg cccgagtcgt tttgccttta acaaccgttt gtgcgattcc
     7441 ctcttccgtc tgatatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca
     7501 gtacaaatag tacggaaccg attcacttgg tgcttcagca ccttagagaa tcgttctctt
     7561 tgagctaagg cgaggcaacg ctgtactggt ttttgttaat ccactataac gcaggaactg
     7621 atgttccctg tcgccgaaat tgctggtaca cgcacacagc agcaatgccg cccacacagc
     7681 cggtttcata cacacctccc attaaagcca aacattatac agccgttctg accgattaaa
     7741 ttcatatttt aaaacaatat tctgtttaca aaacccacat cgtgctataa tccgcaccga
     7801 ttttcagacg gcatcgtcat gccgtctgaa attttttcat tccaacaacc atcagccccg
     7861 cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc gtaccttcgg
     7921 ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg aagaacacgg
     7981 cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca acacctgctc
     8041 cgtgcgcgaa aaagcgcaag aaaaagtctt ctccgatttg gggcgcgtgc gtccgctcaa
     8101 agaaaaaaac cccggcctca tcatcggcgt tgccggctgc gtcgcctcgc aagaaggcga
     8161 aaacatcatc aaacgcgcgc cttatgtgga cgtggttttc ggcccgcaaa cgctgcaccg
     8221 cctgccaaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg atatttcctt
     8281 ccccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg gcgcggcatt
     8341 tgtatcgatt atggaaggct gttccaaata ctgctccttc tgcgtcgtcc cctacacgcg
     8401 cggcgaagaa ttctcccgcc cgctcaacga cgtattgaca gaaatcgcca accttgccca
     8461 gcaaggcgtg aaagaaatca acctcttggg acaaaacgtc aacgcctatc gcggcgaaat
     8521 ggacgacggc gaaatctgcg acttcgccac cctgctgcgc atcgtccacg aaatccccgg
     8581 catcgaacgt atgcgcttca ccaccagcca cccgcgcgag tttaccgact cgattatcga
     8641 gtgctaccgc gacctgccca aattggtttc ccacctgcac ctgccgattc aaagcggttc
     8701 cgaccgcgta ttgagcgcga tgaaacgcgg ctacaccgct ttggaataca aatccatcat
     8761 ccgcaaactg cgtgccatcc gtcctgattt atgcctgagc agcgatttca tcgtcggctt
     8821 ccccggcgag accgaacgcg agttcgagca aaccttgaaa ctggtgaaag acattgcctt
     8881 cgacttgagc ttcgtgttta tttacagtcc gcgccccggc acgcctgccg ccaacctgca
     8941 cgacgacacg ccgcacgaag aaaaagtgcg ccgcctcgaa gccttgaacg aagtcatcga
     9001 agccgaaacc gcgcgcatca accaaaccat gatcggcacg gttcaacgct gcctggtcga
     9061 aggcatctcc aaaaaagacc ccgaccaact gcaagcccgt accgccaaca accgcgtcgt
     9121 caacttcacc ggcacgcccg acatgattaa ccaaatgatc gatttggaaa tcaccgaggc
     9181 ctacaccttc tccctgcgcg gcaaagttgt cgaagcctaa accctcacgc cgaaaaaatg
     9241 ccgtctgaag cgtttcagac ggcattttgc cttgtatcgg cagacgacgg cgcggccggg
     9301 cggcttaatt tgccgcatcc cgatccgaca gccacgcgcg cacacgccgt tccaccgctt
     9361 cggcactcaa gcccaaatcg ttcaaaagtt ttttcggatc gccgtgtccg gttacggtat
     9421 cggcaacgcc caaaagcaaa acgggtttgc agatgccgtg tttcgccaat acttccagca
     9481 ccgcgccgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg cggtcgtggc
     9541 ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacgaagcgc atatcggcga
     9601 cggtggcgtt cagtttttcg gcaaccgcca atgcgggggc gaccatactg ccgaaggcaa
     9661 tgaatgcggt tttctcacct tcgcggcgga taatgccctt gccgatttcc acggtttcca
     9721 tgccgtctga aaccggcgcg cccgtacccg tgccgcgcgg atagcggacg gcggcgggcg
     9781 cgtctgcctg atagcaggtc gaaagcaaca ggcggcattc gttttcatcg ctcggcgcgg
     9841 cgacaatcat gttcggcacg cagcgcaaaa agctcaaatc gtacagaccg gcatgggtcg
     9901 ggccgtccgc gccgacgatg cccgcgcggt cgacggcaaa caaaacgggt aggttttgca
     9961 gggcgatgtc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa taaatcgcca
    10021 cgacgggctt catgccttcg cacgccaaac cgccggcaaa ggtaacggcg tgctgctcgg
    10081 caatgccgac atcgaaataa cggtcgggga atcgttgttc aaactccacc agtccgctgc
    10141 cctcgcgcat cgcgggcgtg atggcgatca aacgctcgtc tgccgccgcc cggtcgcaca
    10201 gccatttgcc gaacacttgg gtataggtcg gtttggcggc gggcttgggt tctttttcag
    10261 acggcatttg cgccgcgctt tctttaggca ggttggcgac ggcgtggtat ttgacgggat
    10321 cgttttcggc gagtttgtag ccgttgccct ttttggtgat gacgtgcaga agctgcgggc
    10381 ctttgcgtcc gcgcaggtct tccaatacat cgaccagatt ttcgacgttg tgtccgtcca
    10441 cggggccggt atagcggaag ccgaagtttt caaacaaaga cagtgactgt ttggcgtgtt
    10501 cggcttcttc ggcaagggtt ttgattttat gttcgacttt ttgggcaaac tccatcgcgc
    10561 cgggtatttt gtctaatacc ttgcccgttt gcgctttgac ggtactcaac aggccgtgca
    10621 tatcgcgcac gacgttgctg gcaaggtatt tcggcagcgc gccgacgttg ggggaaatcg
    10681 acatttcgtt gtcgttgagg acgaccagca aatccacatc catatcgcct gcgcaattca
    10741 aggcttcaaa cgcctgcccc gccgtcatcg cgccgtcgcc gatgatggcg acgctgcggc
    10801 ggtcgctgcc caagagtttg tctgccgccg ccatgcccaa cgccgcgccg atggaggtgg
    10861 aggaatgccc cacgccgaac gcgtcgtact cggactcgca acgtttcgga aaacccgcca
    10921 aaccgccata ttggcgcatg gtgtgcatct ggtttttcct gcctgtcagg attttgtgcg
    10981 gatagctttg gtgtccgaca tcccacacca gcttgtcttc gggcgtgtcg tacacatagt
    11041 gcagggcgat ggtcagttcg accgcgccca aattgctggc gaaatgcccg ccggtctgcc
    11101 cgacagattc cagcagaaag gtgcgcagct cgccggcaag gcgcggcagc tgttttttgt
    11161 ccagacggcg caaatcttgc gggctgtcaa tcaggtcgag tagggggctt gggttcatgg
    11221 tgtgtctttt ttatgtgtcg tccgggtgca acggccaatt atatatcaag agcgtgcggc
    11281 tgacggctga ttttgccgta tgtcattcgt cctgccgctt ggcgcgcggg tgggcttcgt
    11341 catacaggcg ggcgatgtgg tcgaaatcga gtttggtgta aatctgcgtg gtcgaaaggc
    11401 tgctgtgccc gagcagctcc tgcaccgccc ggatgtcgcg cgaagcctgc aacaggtgtc
    11461 cggcgtagct gtggcgcatc atatgcggcg aaacgtgcct gccgtcgccg ttttgcgccg
    11521 cccattgcgc caaacgtttt tggatttggc gttggctcag gcgcgtgccg ttcctgccgg
    11581 taaacagggc tttgccgtcc gatgccgtct gacgcagcgg cagatagttt ttcagggctt
    11641 ccacgctttt gccggtcagc ggcacctgcc gctgcttgcg ccctttgccg gtaacgtgta
    11701 cccacgcctc gtccaaatag acatcatctg cattcaagcc gtgtatctcg ctcacgcgca
    11761 aaccgctgcc gtacatcagt tcgaacagag cgtggtcgcg caccgccagc gggtcgccgc
    11821 cgtccacggg caaatccagc atccggttca gccattcctg cggcagggct ttgggtacgc
    11881 gctcgggctg cttcggcggt ttgatgtcgg cggtcgggtc ggtatgcagc agaccgcgtt
    11941 ccaccagcca aacacaatac tgccgccaag acgacagctt gcgcgccagc gtccgcgcat
    12001 tcaaaccgcg ctgcgacagc cgccgcaacg ccgccgtaaa atcgcgccgc gacaagtcct
    12061 gcggcacgcc gccttcatct tcagacggca tttgtgccaa cagcgcaaac agttcttcca
    12121 aatcgcgccg gtatgccgcc accgtgtgct ccgacttgcc ctcgcgcacg atgttttcca
    12181 aataagcgtc aaaatgcgcc gcaaacccgt ccaaaaccat aaccgcccca cacaaatatc
    12241 aaaaaaacag tgaaaacatt atcataaacg acacaccaaa accccgaacc gcccaaaatc
    12301 cgacccgtcc gcccgccgtt atcgttaaca taaaatcaat gagccgttta tggttttttg
    12361 ccgcaaaaaa cattataatc cgccttattt acctattgcc caaggagaca caaatggcac
    12421 tcgtatccat gcgccaactg cttgatcatg ctgccgaaaa cagctacggc ctgcccgcgt
    12481 tcaacgtcaa caacctcgaa caaatgcgcg ccattatgga agccgccgac caagtcaacg
    12541 cgcccgtcat cgtacaggcg agtgcaggtg cgcgcaaata tgcgggcgcg ccgtttttgc
    12601 gccacctgat tctggcggct gtcgaagaat ttccgcacat ccccgtcgtg atgcaccaag
    12661 accacggcgc atcgcccgac gtgtgccaac gctccatcca actgggcttt tcctccgtga
    12721 tgatggacgg ctcgctgatg gaagacggca aaaccccttc ttcttatgaa tacaacgtca
    12781 acgccacccg taccgtggtt aatttctccc acgcttgcgg cgtatccgtc gaaggcgaaa
    12841 tcggcgtgtt gggcaacctc gaaactggcg aagccggcga agaagacggt gtaggcgcag
    12901 tgggcaaact ttcccacgac caaatgctca ccagcgtcga agatgccgtg cgtttcgtta
    12961 aagataccgg cgttgacgca ttggcgattg ccgtcggcac cagccacggc gcgtacaaat
    13021 tcacccgtcc gcccacaggc gacgtgttgc gtatcgaccg catcaaagaa atccaccaag
    13081 ccctgcccaa tacacacatc gtgatgcacg gttccagctc cgttccgcaa gaatggctga
    13141 aagtcatcaa cgaatacggc ggcaatatcg gcgaaaccta cggcgtgccg gttgaagaaa
    13201 tcgtcgaagg catcaaacac ggcgtgcgca aagtcaacat cgataccgac ttgcgccttg
    13261 cttccaccgg cgcggtacgc cgctaccttg ccgaaaaccc gtccgacttc gatccgcgca
    13321 aatatttgag caaaaccatt gaagcgatga agcaaatctg cctcgaccgc tacctcgcgt
    13381 tcggttgcga aggtcaggca ggcaaaatca aaccggtttc cttggaaaaa atggcaaacc
    13441 gttatgccaa gggcgaattg aaccaaatcg tcaaataaca ggttgcttgt aaacaaaatg
    13501 ccgtctgaac cgttgttcgg acggcatttg atttttgctt ctttgacctg cctcattgat
    13561 gcagtatgca aaaaaagata ccataaccaa aatgtttata tattatctat tctgcgtatg
    13621 actaggagca aacctgtgaa ccgaactgcc ttctgctgcc tttctttgac cgccgccctg
    13681 attctgaccg cctgcagcag cggaggcggc ggtgtcgccg ccgacatcgg cgcggggctt
    13741 gccgatgcac taaccgcacc gctcgaccat aaagacaaaa gtttgcagtc tttgacgctg
    13801 gatcagtccg tcaggaaaaa cgagaaactg aagctggcgg cacaaggtgc ggaaaaaact
    13861 tatggaaacg gcgacagcct caatacaggc aaattgaaga acgacaaaat cagccgcttc
    13921 gactttatcc gtcaaatcga agtggacggg cggctcatta ccttggagag cggagagttc
    13981 caaatataca aacaggacca ctccgccgtc gttgccctac agattgaaaa aatcaacaac
    14041 cccgacaaaa tcgacagcct gataaaccaa cgctccttcc ttgtcagcgg tttgggcgga
    14101 gaacataccg ccttcaacca actgcctgac ggcaaagccg agtatcacgg caaagcattc
    14161 agctccgacg atgctggcgg aaaactgacc tataccatag atttcgccgc caaacaggga
    14221 cacggcaaaa tcgaacacct gaaaacaccc gagcaaaatg tcgagcttgc cgccgccgaa
    14281 ctcaaagcag atgaaaaatc acacgccgtc attttgggcg acacgcgcta cggcagcgaa
    14341 gaaaaaggca cttaccacct cgcccttttc ggcgaccgcg cccaagaaat cgccggctcg
    14401 gcaaccgtga agataaggga aaaggttcac gaaatcggca tcgccggcaa acagtagcat
    14461 ttgaaaaatg ccgtccgaac acgataattt accgttcgga cggcattttt gcgccatacc
    14521 gccctaggat acgacggcac ggcaggcaag ccggtttcac tatataatgc cgtctgaacc
    14581 aacgagaacc acaccatgca agccgatttc aaccgccccg tcctcgccgt cgataccgga
    14641 acttcctatt tgtcgctcgc gctgcgcgcc gacggcgaaa ccctcctgtt ccatcaggag
    14701 gccggcagcc gccagtccga actgatcctg ccggaaatcc gcaccctatt ccgaaatgcg
    14761 ggcattaccg ccgccgattt gggggcgatt gtttatgcga aaggtcccgg cgcgtttacc
    14821 ggactgcgta tcggcatcgg tgtggctcag ggtttggcaa cgccgtttga tacgccttta
    14881 atcggcatcc ccacgctcga tgccgccgcc tcgatgccgc cgccgcaaag ctgcatactc
    14941 gccgctgcgg acgcgcgtat gggcgaagtg ttttatgcgt ggttcgacac gctgaaccgc
    15001 cgccgtttga gcgattatca ggtcgggcgg gcggcagaca tccggctgcc ggagggatgt
    15061 gccttttcag acggcatagg cagcgcgttc gcgttggaag aaacgccgcc gttttcaggc
    15121 aaacccgata tgccgaccgc cgccgacttc ctcaatctgg cggcggaagg cggttttccc
    15181 gccgtcgctg ctgcacacgc cgaactgctc tacgtccgca acaaaatcgc cctaaccgcc
    15241 aaagaacagg cggaacggaa agcccgaaca tgaacatccg ccgcgcctca ccgtccgact
    15301 gcgccgcact cgccgcgctc gatgccgtct gcaacccgtc cgcgtggacg caacgccaat
    15361 ttgagtccgc attggtttcg ccgtccgaac aggttttcct cgcggaaaaa gacggcggga
    15421 ttgccgcctt tatcgtttgg cagaacctgc ccgacgaatc cgaactgcac ctgattgcca
    15481 ccgcgcccga atgccgccgc cgtggcgttg cgtccgccct gctcgaatat tggttcgcac
    15541 atctgcccga agacacgcaa cgcctgctgc tcgaagtccg tgcaggcaac accgccgcac
    15601 aggcactgta cgcggcgcac ggctttaccg atgcgggcag gcggaaaaac tattaccgta
    15661 cagccgacgg taaaaccgaa gatgctgtct taatggagaa aatatgttaa gcgcgcgcta
    15721 cctccacctg cacgaagctt tgggtttagg cccgatgtgg ctgaaacagg ccgccgccgt
    15781 cctgccgccc aaaaacacac ccgcaacccc ggcacaggca cgtcctcaaa ccgtccgcgc
    15841 cgccccgatc cgcccttccc aaccccataa cggtcaggcg cggctcgaaa cgatgaaagc
    15901 gttggaaacc gccgccgtac ctacgcgcaa acccgcgcct gaaaccgaaa cgcctctgcc
    15961 cggcctttca gacggcatcg cccccgttcc cgccgcttcg ggcatcacca agcttgccgt
    16021 cgtcagcctt tgcccaccga tcgaggatgc ggtttacggg caactgttcc acggcaaagc
    16081 gggtgtcctg ctcgacaaca tactcaaagc cgtaggactg gatgccgctt atgtacacaa
    16141 aacctgttgg gtgaaaaccg ccgccgtcgg caacccgatg ccgtctgaac aggccgtcgc
    16201 ggatgcgctg ggtcaaatcg cccgcgaact cgacagttgc cgcgcccccg ccgtcctctt
    16261 cctcggacag gcttttgtcc agcctgaacg gcaaacgatg attgaaactt tgtgcggcag
    16321 ccgtcccttc ttcatcatcg accatcccgc ccggctgttg cgccaacccg aactcaaagc
    16381 ccgcacctgg caggtgttga aacagttgaa acacgccttg caggcaggcg gcagttgaag
    16441 caggccgcac ggggcggtag aatcacaact gcatcgcaat acctgacaga aagcaaaaaa
    16501 tgaccgattt ccgccaagat ttccttaaat tctccctcgc ccaaaatgtc ttaaaattcg
    16561 gcgaatttac caccaaggca ggacggcagt cgccttactt cttcaatgct ggcctcttta
    16621 acgacggctt gtccacgctg caactggcaa aattctatgc acaatccatc attgaaagcg
    16681 gcatccgatt cgatatgctg ttcggccccg cctacaaagg cattattttg gcggcggcaa
    16741 ctgcgatgat gctggcggaa aaaggcgtga acgtcccgtt cgcctacaac cgcaaagaag
    16801 ccaaagacca cggcgaaggc ggcgtgttgg tcggcgcgcc gcttaaaggg cgcgtgctga
    16861 ttatcgacga cgtgatttcc gccggcacat ccgtacgcga atcgatcaaa ctgattgaag
    16921 cggagggtgc aacccccgcc ggtgtcgcca tcgcgctcga ccgtatggaa aaaggcacgg
    16981 gtgaattgag cgcggttcag gaagtggaaa aacaatacgg cctgcccgtc gcccccatcg
    17041 ccagcctgaa cgatttgttt attctgttgc aaaacaaccc cgaattcgga cagttcctcg
    17101 aacccgtccg agcctaccgt cggcagtacg gcgtagaata aaaacaaagc atatgccgtc
    17161 cgaaccgcct tacgcctcag acggcatcaa acctgacaca cacgaggaaa taccatgccc
    17221 gcctgtttct gcccccactg caaaacccgt ctctgggtca aagaaaccca actcaatgtc
    17281 gcccaaggct tcgtcgtctg ccaaaaatgc gaaggactgt ttaaagccaa agaccatctg
    17341 gcaagcacga aagaacccat attcaacgat ttgcccgagg ctgtttcgga tgtcaaactc
    17401 gttcaccgta tcggcacgcg cgccatcggc aagaaacaga tttcccgtga cgaaatcgcc
    17461 ggcatcctca acggcggcac aacccagccc gatattccgc ccgcaaccgc cgccacccct
    17521 gctgccgcac cgcaggttac cgtaccgccc gccgcgcccg cccgtcagga tgggttcaac
    17581 tggacgattg caaccctgtt tgcccttatc gtcctcatta tgcagctttc ctacctcgtc
    17641 atcctatgag cgcgcccgac ctctttgtcg cccacttccg cgaagccgcc ccctacatcc
    17701 gccaaatgcg cggcaaaacg ctggtcgccg gcatagacga ccgcctgctc gaaggtgata
    17761 ccttaaacaa gctcgccgcc gacatcgggc ttttgtcgca actgggcatc aggctcgtcc
    17821 tcatccacgg cgcgcgccac ttcctcgacc accacgccgc cgcgcaaggc cgcacgccgc
    17881 attattgcag gggcttgcgc gttaccgacg aaacctcgct cgaacaggcg cagcagtttg
    17941 ccggcaccgt ccgcagccgt tttgaagccg cattgtgcgg cagcgtgtcc gggttcgcgc
    18001 gcgcgccttc cgtcccgctc gtatcgggca acttcctgac cgcccgtccg ataggtgtga
    18061 ttgacggaac cgatatggaa tacgcgggcg ttatccgcaa aaccgacacc gccgccctcc
    18121 gtttccaact cgacgcgggc aatatcgtct ggctgccgcc gctcggacat tcctacagcg
    18181 gcaagacctt ctatctcgat atgcttcaaa ccgccgcctc cgccgccgtc tcgcttcagg
    18241 ccgaaaaact cgtttacctg accctttcag acggcattgc ccgccccgac ggcacgctcg
    18301 ccgaaaccct ctcggcgcag gaggcgcaat cgctggcgga acacgccggc agcgaaacgc
    18361 aacggctgat ttcgtccgcc gttgccgcgc tcgaaggcgg cgtgcatcgc gtccaaatcc
    18421 tcaacggggc cgccgacggc agcctgctgc aagaactctt cacccgcaac ggcatcggca
    18481 cgtccattgc caaagaagcc ttcgtctcca tccggcaggc gcacagcggc gacattccgc
    18541 acatcgctgc cctcatccgc ccgctggaag aacagggcat cctgctgcac cgcagccgcg
    18601 aatacctcga gaaccacatt tccgaatttt ccatcctcga acacgacggc aacctgtacg
    18661 gttgcgccgc cctgaaaacc tttgccgaag ccgattgcgg cgaaatcgcc tgccttgccg
    18721 tctcgccgca ggcacaggac ggcggctacg gcgaacgcct gcttgcccac attatcgata
    18781 aggcgcgcgg cataggcata agcaggctgt tcgcactgtc cacaaatacc ggcgaatggt
    18841 ttgccgaacg cggctttcag acggcatcgg aagacgagtt gcccgaaacg cggcgcaaag
    18901 tctaccgcag caacggacgg aactcgcata ttctggtgcg tcgcctgcac cgctgaccgc
    18961 aacggaaagc cgccgcagaa atgccgtctg aaccccgttt cagacggcat ttccccgatt
    19021 ataaagacct gcccaaccct caaggacccc gatgaaatcc taccccgacc cctaccgcca
    19081 ttttgaaaac ctcgattccg ccgaaacgca aaacttcgct gctgaagcga atgccgaaac
    19141 gcgcgcgcgt tttttaaaca acgacaaggc gcgcgaattg tccgacggca ttttggcgca
    19201 gttgcaggac acgcggcaga ttccgttttg tcaggaacac cgcgcgcgga tgtaccattt
    19261 ccatcaggac gcagaatatc cgaagggcgt gtaccgcgtg tgtaccgcgg cgacgtatcg
    19321 ttccggctat cccgagtgga aaatcctgtt ttcggtggcg gatttcgacg aattgcttgg
    19381 cgacgatgtg tatttgggcg gcgtgtcgca cttggtggaa cagcccaacc gcgcgttgtt
    19441 gacactgagc aaatcgggcg gcgatacggc gtacacactg gaagtggatt tggaagcaag
    19501 ggaattggta aaaggcggtt ttcactttcc ggcaggcaaa aaccatgtgt cgtggcgcga
    19561 tgaaaacagc gtgtgggtgt gtccggcctg ggacgaacgc cagttgaccg aatcgggcta
    19621 tccgcgcgaa gtgtggctgg tggaacgcgg caagagtttc gaggaaagcc tgccggtgta
    19681 ccaaatcggc gaagacggga tgatggtgaa cgcgtggcgt tatctcgatc cgcagggttc
    19741 gccgattgat ttgattgaag cgtcggacgg tttttacacc aaaacctatt tgcaggtgtc
    19801 agccgaaggc gaggcgaaac cgttgaacct gcccaacgat tgcgacgtgg tcggctatct
    19861 ggcggggcat cttttgctga ccttgcgcaa ggactggaac cgcgcgaacc aaagctatcc
    19921 gagcggcgcg ctggtggcgg tgaaactgaa tcgcggcgaa atcggggcgg cgcagctttt
    19981 gtttgcgccc gatgaaacac aggcattgga aagcgtggaa acgaccaagc gttttgtggt
    20041 ggcgagcctg ctggaaaacg tgcaaggccg tctgaaggct tggcgtttta ctgacggcaa
    20101 atggcaggag gccgagttgc cgcgcctgcc ctcgggcgcg ttggaaatga ccgaccaacc
    20161 gtggggcggc gacgtggttt accttgccgc cggcgatttc actacgccgc tgacgttatt
    20221 tgcgctggat ttgaacgtga tggaactgac cgttatgcgt cgccagccgc agcagtttgt
    20281 ttcagacggc atcaacgtgc ggcagttttg gacggcttcg gctgacggcg agcgcatccc
    20341 ttatttccac gtcggcaaaa acgccgcgcc cgacacgccg actttggttt atgcctacgg
    20401 cggtttcggt gtacccgagc tgccgcatta tctgggcagc atcggcaaat actggctgga
    20461 agagggcaac gccttcgtgt tggcgaacat ccgcggcggc ggcgagttcg gcccgcgctg
    20521 gcatcaggcg gcgcagggca tcagcaaaca caaaagtgtc gacgatttgc ttgccgtcgt
    20581 gcgcgatttg tccgaacgcg gcatgagttc gcccagacac atcggtttgc agggcggcag
    20641 caacggcggc ctgattaccg ccgctgcctt cgtgtgcgaa ccgcaaagca tcggcgcgct
    20701 ggtgtgcgaa gtaccgctga ccgacatgat ccgttatccg ctattgtccg ccggttcaag
    20761 ttggacggac gaatacggca atccgcaaaa atacgaagtc tgcaaacgct ggctgggcga
    20821 attgtcgccg tatcacaatc tttcagacgg catcgattat ccgcctgcgc tcattaccac
    20881 cagcctcagc gacgaccgcg tccatcctgc ccacgcgctc aagttttacg ccaaactgca
    20941 tgaaacctcg gcgcaatctt ggctctactc gcctgacggc ggcggccata ccggcaacgg
    21001 cacccaacgc gaatccgccg acgaactcgc ctgcgtcttg ctgtttttga aagagttttt
    21061 gggctaaggg cggggagcgg cactgccgcc gcgaatgaaa aaaggtcgtc tgaaactgct
    21121 ttttcagacg acctttttta atggttgttt caaatcaaaa tatctatgcc gccggcccca
    21181 tcagcacttc ttcacatccg aaggcaaaaa tccgtaatgc cgtctgaacg cttcgttgaa
    21241 ccgtcccgcg tggcggtagc cgcaaaagtg catggcggct tggacggtgc tgccggattc
    21301 gatgagggcg agcgcgtgtt ccagccgcag gcggcgcagg catccggcga cggtttcacc
    21361 ggtttgcgct ttgaaatagc gtttcaggta gcattcgttc agtccgacgc ggcgggcgat
    21421 ttcggcgatg gtcagcggac gggcgaattc gtgttgcagg atgtcggcgg cttcgtctat
    21481 gcgccggcgg cggtaaccgt tgtcgtggcg gcggaaggtg aagcgcaata atcgggcgga
    21541 gagttccagc gcggcggctt cgtcggcaag caggccgaag ccgtccgatt cgaacgggcg
    21601 ttgcagcagt gggcaggccg ccgccgtcag tgccgctgcg ttttgcgcca gccgttgcag
    21661 ggcgaatcgg cctattgttt gcggcgaaaa caggcgttcg tccagcaagc cttcgtcgtg
    21721 ccagcggcgc agtttttcca gcgaaaaatc caaatgcagc gcgcacatgc cgctgttgtc
    21781 gggcagcagg gtttcggata cgtccgccaa ctcgccgcgt accagccaga tttcgccggc
    21841 agacgggcgg tattccctgc cgcccatttg taaccggttc tgccccgaca ccatgacgaa
    21901 caaggcgcag ttgtggctga aattgtggat ttcggtggga aacgcgcccg ttccgccgcc
    21961 gcgcatccgc gacaaggtga tgcccgagtc gaagcggttg atgcacattt ccagatgcaa
    22021 accgggctgt tttgcctgcg caatgagcgc gctgtcggaa cagccgtcca acgcccagcc
    22081 ggatttatcg gagcggacat aggtttggta ctggcggtag atggcggcgg tgttcatgat
    22141 tggataggaa cgagttgtct aacaaatgaa ttaaatagga attattacca ataatcaagc
    22201 gcagggattg gttgaaacgg aaaaggtcgt ctgaaagggt gtttcagacg accttttccg
    22261 tatcgggaat ttgttttgcc gtatcgggaa ttttgcgttt tgcggcgtgg tttctgcagg
    22321 ttgtttgctt aataataaac attcttattc gtatgcaaag gaaccgcaca ccgtgaaacc
    22381 gcgtttttat tgggcagcct gcgccgtcct gctgaccgcc tgttcgcccg aacctgccgc
    22441 cgaaaaaact gtatccgccg catccgcatc tgccgccacg ctgaccgtgc cgaccgcgcg
    22501 gggcgatgcc gttgtgccga agaatcccga acgcgtcgcc gtgtacgact gggcggcgtt
    22561 ggatacgctg accgagctgg gcgtgaatgt gggcgcaacc accgcgccgg tgcgcgtgga
    22621 ttatttgcag cctgcatttg acaaggcggc aacggtgggg acgctgttcg agcccgatta
    22681 cgaagccctg caccgctaca atcctcagct tgtcattacc ggcgggccgg gcgcggaagc
    22741 atatgaacag ttggcgaaaa acgcgaccac catagatctg acggtggaca acggcaatat
    22801 ccgcaccagc ggcgaaaagc agatggagac cttggcgcgg attttcggca aggaagcgcg
    22861 cgcggcggaa ttgaaggcgc agattgacgc gctgttcgcc caaacgcgcg aagccgccaa
    22921 aggcaaagga cgcgggctgg tgctgtcggt tacgggcaac aaggtgtccg ccttcggcac
    22981 gcagtcgcgg ttggcaagtt ggatacacgg cgacatcggc ctaccgcctg tagacgaatc
    23041 tttacgcaac gaggggcacg ggcagcctgt ttccttcgaa tacatcaaag agaaaaaccc
    23101 cgattggatt ttcatcatcg accgtaccgc cgccatcggg caggaagggc cggcggctgt
    23161 cgaagtattg gataacgcgc tggtacgcgg cacgaacgct tggaagcgca agcaaatcat
    23221 cgtcatgcct gccgcgaact acattgtcgc gggcggcgcg cggcagttga ttcaggcggc
    23281 ggagcagttg aaggcggcgt ttgaaaaggc agaacccgtt gcggcgggga aagagtaggg
    23341 agtcgtctga aaacggagct tccgaaggaa gcgggggggt ttctgcgaag ctaaagtgcg
    23401 gtttcaacga attgaaaagc agcctgtatg ttgaaaatac cgctcaagca aacctacggt
    23461 ttgccgccct ctccctagcc ctctcccaca gggagagggg attggggtgc aggctgtctt
    23521 taaggttcag gcaaattttt aacttcgttg aagctccgtt ttcagacgac cttttggag
//
LOCUS       DAWFJR010000023        22947 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWFJR010000023 DAWFJR010000000
VERSION     DAWFJR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22947
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.023"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(93..932)
                     /locus_tag="WGR04_000841"
     CDS             complement(93..932)
                     /locus_tag="WGR04_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ0794428.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADGAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1217..1292
                     /locus_tag="WGR04_000842"
     tRNA            1217..1292
                     /locus_tag="WGR04_000842"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1250..1252,aa:Arg,seq:ccg)
     gene            complement(1357..2550)
                     /locus_tag="WGR04_000843"
     CDS             complement(1357..2550)
                     /locus_tag="WGR04_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0794429.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2571..3659)
                     /gene="trmA"
                     /locus_tag="WGR04_000844"
     CDS             complement(2571..3659)
                     /gene="trmA"
                     /locus_tag="WGR04_000844"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ0794430.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASMIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDDEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSISAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLVETH
                     AVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            3802..4902
                     /gene="aroC"
                     /locus_tag="WGR04_000845"
     CDS             3802..4902
                     /gene="aroC"
                     /locus_tag="WGR04_000845"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ0794431.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDGVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAVDTPDIARSDK"
     gene            4993..5418
                     /locus_tag="WGR04_000846"
     CDS             4993..5418
                     /locus_tag="WGR04_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ0794432.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDAALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5492..7477)
                     /gene="parE"
                     /locus_tag="WGR04_000847"
     CDS             complement(5492..7477)
                     /gene="parE"
                     /locus_tag="WGR04_000847"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ0794433.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPIFSCENYISDGHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVAGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7544..8068)
                     /locus_tag="WGR04_000848"
     CDS             complement(7544..8068)
                     /locus_tag="WGR04_000848"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ0794434.1"
                     /translation="MLDREGYRPNVGIILINERNEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQLSGNR"
     gene            8233..9528
                     /gene="serS"
                     /locus_tag="WGR04_000849"
     CDS             8233..9528
                     /gene="serS"
                     /locus_tag="WGR04_000849"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ0794435.1"
                     /translation="MLDIQLLRSNTAAVAERLAARGYEFDAARFNALEEQRKAVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVADSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRLKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9601..10599)
                     /locus_tag="WGR04_000850"
     CDS             complement(9601..10599)
                     /locus_tag="WGR04_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0794436.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEAEKIGGRYVELNELYARSDIITLHCPSTPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSSAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10724..11800)
                     /gene="prfA"
                     /locus_tag="WGR04_000851"
     CDS             complement(10724..11800)
                     /gene="prfA"
                     /locus_tag="WGR04_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ0794437.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDADDDKNIFIEVRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITNALIAEHQAELLAAMGD"
     gene            complement(11890..12669)
                     /locus_tag="WGR04_000852"
     CDS             complement(11890..12669)
                     /locus_tag="WGR04_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0794438.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAAREAGVGRFIVPATRPQDW
                     QDVADLAQRLSENPLWGNIHIALGIHPWFSDDVSEPDFQRLEQALKARPMAWVGEIGL
                     DFYDKTQTPQQRERQIQVFSRQLAIAQTLRRRVIIHNLKASNEIAAAVRQTGFSCGGI
                     VHAFSGSIEEARVLVGLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLK
                     NTVNTPANILRIAEIAAQIRGTGAAEIAAITERNADSLLRP"
     gene            complement(12784..13773)
                     /locus_tag="WGR04_000853"
     CDS             complement(12784..13773)
                     /locus_tag="WGR04_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ0794439.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGPLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHNVRIPNQDAADVSDGLKIHYPDPQAKIAVRTLIPG
                     FAVQELADGLGRLSAQALILQSYGHGNTPADEGFIRAVRDFTRQGKLLLNISQVRQGK
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGKEEVEKELARLKL"
     gene            complement(13840..14523)
                     /locus_tag="WGR04_000854"
     CDS             complement(13840..14523)
                     /locus_tag="WGR04_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0794440.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14711..16045)
                     /gene="glmM"
                     /locus_tag="WGR04_000855"
     CDS             complement(14711..16045)
                     /gene="glmM"
                     /locus_tag="WGR04_000855"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ0794441.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEVELEIEAKIDGEMKTQPSARLGRARRINGADDRY
                     IEFCKSTFPTHSDLRGLKLVVDAANGAAYSVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIGKGQNWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIAAAIGSL"
     gene            complement(16175..17032)
                     /gene="folP"
                     /locus_tag="WGR04_000856"
     CDS             complement(16175..17032)
                     /gene="folP"
                     /locus_tag="WGR04_000856"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ0794442.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRSGADYVSPEEEWARVEPVLAEVAGWGVPISLDTRR
                     TVIMEKALALGGVDIINDVAALNDEGAVELLARQADTGICLMHMQGLPKTMQINPKYQ
                     DVVGEVARYLKARSAECIAAGIAPQRIILDPGFGSGFGKPLQHNIALMRHLPELMAET
                     GFPLLIGVSRKSTIGELTGEANAAERVHGSVAAALASVARGAQIVRVHDVKATADALK
                     VWEALGINL"
     gene            complement(17172..18065)
                     /locus_tag="WGR04_000857"
     CDS             complement(17172..18065)
                     /locus_tag="WGR04_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ0794443.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVCIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            18156..20270
                     /locus_tag="WGR04_000858"
     CDS             18156..20270
                     /locus_tag="WGR04_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ0794444.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYAQWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKGHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTASGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(20335..21813)
                     /gene="ubiD"
                     /locus_tag="WGR04_000859"
     CDS             complement(20335..21813)
                     /gene="ubiD"
                     /locus_tag="WGR04_000859"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ0794445.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFEHPVKPNGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGENVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVDVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(21834..22028)
                     /locus_tag="WGR04_000860"
                     /pseudo
     CDS             complement(21834..22028)
                     /locus_tag="WGR04_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
     gene            22027..22173
                     /locus_tag="WGR04_000861"
     CDS             22027..22173
                     /locus_tag="WGR04_000861"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794446.1"
                     /translation="MQKNRQLAAPTAKPDGLYLVDVIYPQKFDIPKRPIGPLFLEDGL
                     LNRT"
     gene            complement(22258..>22947)
                     /locus_tag="WGR04_000862"
     CDS             complement(22258..>22947)
                     /locus_tag="WGR04_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEZ0794447.1"
                     /translation="ALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAEPVTKAESHKV
                     SVAIAVVVIFGTLAIFTYPLFYTWSQHLINAHQFGIYVGSSVHEVAQVYAIGENIDPI
                     VANTAVISKMIRVMMLAPFLLMLSWLLTRSNGVSENTSHKITIPWFAVLFIGVAIFNS
                     FDLLPKELVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVVGG
                     FLVNYGISKLI"
BASE COUNT         5226 a         6471 c         5993 g         5257 t
ORIGIN      
        1 tcctgatttt tgttaatcca ctataaattc ccataaaaaa acggagcaga tacctgcccc
       61 gtttttattt aatccgaaat tttaatctaa atttagaatt ttgcaccgga ttggtttgcc
      121 atatagtcaa cagccgcttt gacttcgtca tcgctcaaac cggcattgcc gcctttggca
      181 ggcatcgcgt taaagccttc aagggcgtgt ttgtgcaagg tttctttgcc ttttttgata
      241 cgcggtgccc aatcgtcttt tttgcctatg ccgggaatac cgggaatcga accgccgtgg
      301 cacacctgac aggttgcttc gaagactttt ttaccgtcaa cgccgaccgc aggggctgcc
      361 gcacccttgt cttctgcctt cgcttctgcc ggagctgcac catcggcagg agcagaagct
      421 gttcctgaag cggcattgtc ggcaggcgca gcctcatcag gattcgggaa agaaccgccg
      481 cttttgttcg ccatgtaagt aatcgcccgt ttgagttcct gatcggtcag gtctgccgca
      541 ccgcctttgg caggcatggc gttaaagccg ttcagcgcgt gttggaacaa ggtatcgaag
      601 ccttgcgcga tacgcggtgc ccaatcgccg ttgtgttcca gtttcggagc gttcggcaca
      661 ttgctgtccg ccgcgtggca ttggatacag attttgccga aaatctgttc gccttggcgt
      721 tcgccgacgg ggatgccgtc gcccatcgtc aattgtccga caggctggat acgggtctgc
      781 gttgctgctt ccgtagtggc atcgacatcg ccgaacgagc cgctgcccgc cagcttaatc
      841 aggaaataaa ggactgcaat aacaataacg ataccgctca caagggtaaa cagtgcagag
      901 ccttgggctt tgttgtcgcg gagttgtttc atttggtagg cctcgccgtc aggttaggtt
      961 gtgctgtaaa ttatagtttg gtgtgttaaa cgcagttaac aatattttgc tggattatac
     1021 tgaattcaca gggtctttcc aatcgctatc attgaaaata tgaaaaaatt tgccaacggt
     1081 atctgtataa aacaaataat cctttgaaaa taatggttta tcctcaagaa aactctcctt
     1141 atgccgccat acgccgcctg ccggcgcaag ataacctttg ccaatttgca gaatttacgt
     1201 taaccttacg ttttccgcac ccatagctca gttggaagag tgtcagtttc cgaagctgga
     1261 ggtcacaggt tcgatccctg ttgggtgcgc caattataaa gagaccgtct gaaagataaa
     1321 tatttttcag acggtctttt gacttacttc aaactcttat ttcaagactt ccgcaaatgc
     1381 gcgggcaaca tagtcggtat tcgacgtatt cagtccggcg acgcacatcc tgccggaatc
     1441 cagcaggtaa acggcaaatt cgtcgcgcag cctgcggact tgttccacgc tcaatcctgt
     1501 gtagccgaac atgccgcgct gtttgatgaa ataagtgaaa tcgcgatcgg ggatttgcgc
     1561 agttaagaca tcataaagtt tctgccgcat cgcacggatg cggtcgcgca tcatataaac
     1621 ctcgttttgc cacaaggcgt aaagttcggg gctgttcatc acgtcggcgg cgatatacgc
     1681 gccgtgcgcg ggcgggctgg agtagatgcg gcggacggtg aatttgagct gtccgaacac
     1741 caaatccgct tcttccttat tcgggcaaac cacgcttaag ccgccgacgc gctcgccgta
     1801 gagcgacagg ttttttgaga aggaattgct gacgaacaag ggcaattcca tttccaccgc
     1861 tttgcggacg gcgtaggcat cgctgtccaa atcgccgccg aagccttggt aggcaatgtc
     1921 cataaacgga atcagtttgc gcgttttgat gatgtgcaac acttcgtccc attgttgttc
     1981 cgacatatcc acgccggtcg ggttgtggca gcagggatgg aggatcagga cgctgttttc
     2041 gggcagggtg ttgaaaaacg cggtcatttc gtcgaatttc acgccaacag tggcagggtc
     2101 gtaatatggg taagtgccga cctcgaaacc tgcgccttca aaaatgccgc gatggttgtc
     2161 ccaagtcggg tcgctgacgt aggcgcgcgc ttcgggaaac cagcggtgca ggaagtccgc
     2221 cccgactttg agtgcgcccg agccgcccaa ggtttgtacg gtaacgatgc gcccttgcgc
     2281 aagcgcggga ttgtctttgc cgaacaacaa atgctgcacc gcgctgcggt aagtgtccaa
     2341 gccttccatc ggcaggtagg gcgacggcgc aggcgcggcg gcacgggcag tttcggcgcg
     2401 gctcacggat tccaacacgg gcattttgcc ttcgtcgtcg aaataaatgc ctatgctcaa
     2461 attgactttt tcggggcgcg ggtcgttttt gaaggtttcg accaaactca aaatcgggtc
     2521 gccgggatag tattcgatgt gtcggtacat agtccttacc ttttactttt tcaaaggatt
     2581 ttctttttca acaatacacc actttcgata tggtgcgtaa acgggaattg gtcgaacagg
     2641 gcggcgcgtt cgaccgcatg ggtttccacc aaggtgtcca aattggcacg cagcgtttcg
     2701 ggattgcagg aaatgtagat gatgttgtca aactgcgaca ccagcttcaa agtttcctca
     2761 tcgataccgg cacgcggcgg atcgacgaaa atagtggaaa atgcgtaatc cgtcaaagca
     2821 ataccgccat ccttaaggcg tttaaactca cgttttccgg tataggcttc ggtaaattct
     2881 tcagcagaca gacgggcgat tttgatgttg ccgatgcggt tggcttcgat attccattgc
     2941 gccgcgctga tggaggtttt ggagatttcg gttgccaaaa cacgttcaaa cttttcagat
     3001 aaaggaagcg taaagttgcc gtttccgcaa tacagttcga gcaaatcccc atccaatcct
     3061 tctgctgcac cgcaggccca ttcaagcatt ttctgacaca cggcggcatt cggttgggta
     3121 aaactgcctt caatttgccg ataacggaaa tcccggttgc cgaccttcaa agtttccgtt
     3181 acatagtcct gttttaagac tattttctgt cccctgctcc gcccaataac ggaaatatcc
     3241 aactgttgct gtaacgcttg cgccgcctgc atccactcat catcaagcct tttgtggtaa
     3301 atcatggtaa ccagcatttc cccgctgagc gtagacaaaa attcgacggc ataccaacgg
     3361 tttctcagtt cggcagattg ggcggcaacg gcaatcaatt ctggcatcag gcggttgacg
     3421 gcttcggaag ccgcttcaaa acggtcgcag cgtatcatgc ttgcgccgct ggctttctgc
     3481 cctttttcaa acatggcata aaacatttcc ccgccttcgt gccaaatacg gaactcggca
     3541 cgcatacggt agtgtttgtc cggagattcg tacacttccc actcaggaac atccaaacct
     3601 gcaaaaaggg ttttaaggtg gtctttttta tcttgaagct gctgcgtgta gtcgctcata
     3661 tcgctatcct gaaaaatcag accggattat aagccgattc actgcaaaac acgcaacccc
     3721 tttaccggca tataacccgt tttcctccaa cttttatgct aaaatccgcc gtctccaaca
     3781 taccgccgac agaaggaaaa tatggcaggc aacactttcg gacaactctt caccgtgacc
     3841 acgttcggcg aaagccacgg cgcaggtttg ggctgcatca tcgacggctg cccgcccggc
     3901 ttggaattaa gcgaagcgga tatccaattt gacctcgacc ggcgcaaacc cggcaccagc
     3961 cgccacgtta cccaacgccg cgaagccgac caagtcgaaa tcctctccgg cgtattcgaa
     4021 ggcaaaacca ccggcacacc catcgccctc ttaatccgca ataccgacca gcgcagcaaa
     4081 gactatggca acatcgccac cagcttccgc cccggccacg ccgactatac ctattggcac
     4141 aaatacggca cgcgcgacta ccggggcggc ggcaggagtt ccgcccgcga aaccgccgcc
     4201 cgcgttgctg ccggagccgt tgccaaaaaa tggttgaaag aaaaattcgg cacggaaatc
     4261 accgcctacg ttacccaagt cggcgaaaaa gaaatccggt ttgaaggctg cgaacacatt
     4321 tcccaaaatc ctttttttgc cgccaaccat agccaaattg ccgagctgga aaactatatg
     4381 gacggcgtgc gcaaatcctt ggattccgtc ggcgcgaagc tgcatattga agccgccaat
     4441 gtccctgtcg gcttgggcga acctgttttt gaccgcctcg atgccgaaat cgcctacgcg
     4501 atgatgggca tcaacgccgt caaaggcgtg gaaatcggcg caggttttga cagcgtaacg
     4561 caacgcggca gcgaacacgg cgacgaactg accccgcaag gcttcctgtc caaccattcg
     4621 ggcggcatcc tcggcggcat cagcaccggg caagacatct gcgtcaatat cgccatcaaa
     4681 cccaccagct ccatcgccac cccgcgccgc agtatcgaca tcaacggcaa ccccatcgaa
     4741 ctcgccacgc acggcaggca cgacccctgc gtcggcttgc gcgccgcgcc gattgccgaa
     4801 gctatgctcg ccttggtttt aatcgaccac gccctgcgcc atcgtgcgca aaatgcggat
     4861 gttgccgtgg atacgcccga tatcgcccgc tcggacaagt aaaaagattt agccaaaaca
     4921 cgggctttat actaaaatac cgaccattgc cgtgccgtct gaacgcacga ttcttagagg
     4981 aatacaaccg aaatgacaca agaaaccgct ttgggtgcgg cactgaaatc cgccgtccaa
     5041 actatgagca aaaagaaaca gaccgaaatg atcgccgacc acatctacgg caaatacgat
     5101 gtgttcaaac gcttcaaacc gttggcactc ggcatcgatc aggatttgat tgccgctttg
     5161 ccgcaatacg atgccgcact gattgcacgc gtcctcgcca accactgccg ccgtccgcgc
     5221 tatctgaaag ccttggcgcg cggcggcaaa cgtttcgatt tgaacaaccg tttcaaaggc
     5281 gaagttaccc ccgaagaaca ggcgatcgcg caaaaccatc ctttcgtgca gcaggctttg
     5341 caacagcagt ccgcccaagc tgccgccgaa acgccgtctg ttgaagccga agcagcagaa
     5401 tcttccgcag cagaataaat ccccaaatga aatgccgtct gaaaaccgat ttggtttcag
     5461 acggcatttt tccgtatgcg gcaatcacgg ttcaaatatc gagttccgcc gtatcgcctt
     5521 cgcgctccat ccaggcacgg cgggcggcgg cttcgccttt gcccatcagt ttgacgaaga
     5581 tgtcgcgcgt ctcgtcatcc gcgccttcgg ggatttgtac ctgcaacagg cggcgggtgt
     5641 cggggtgcat ggtagtatct ttgagctggt ctgggttcat ctcacccaaa cctttgaaac
     5701 ggctgatgga ataagcagtt tctttaacgc cttctttttg cagccgctct aaaatgccgt
     5761 caagttcgtt ttggtcgagg gcgtagaatt tgcgggcggg tttgctttta ccttgtgcgt
     5821 tgacatctac gcggaacagc ggcggctggg cgacgtagat gtgtccgtca gcgaccagtt
     5881 tcgggaagtg gcggtagaac agggtcagca gcaaaacttg aatatgcgag ccgtccacgt
     5941 cggcatcgga caggatggcg attttgccgt aacgcaggcc gcttaaatcg ggatggtcgt
     6001 tgatgccgtg cggatcgacg ccgatggcaa ctgaaatgtc gtggatttcg gcgttgccga
     6061 agagctggtc ggggtggact tcaaagctgt tgagcacttt gccgcgcagg ggcaggatgg
     6121 cctgggtggc tttgtcgcgg gcgagtttgg ctgagccgcc ggcggaatcg ccttcgacaa
     6181 ggaagagttc gttttcgcgg atgtcttcgc tttcgcagtc ggtcagcttg ccgggcagga
     6241 cggcgacgcc gctgcctttt ttcttttcga ttttcttaac cgaacgcatc cgcgcctgcg
     6301 cctgacggat ggcgagttcg gcaatttttt taccgaagtc cacgttttgg ttcagccaca
     6361 attccaaagg gtcgcccgct acagcggcga caagtttcag cgcgtcgcgg ttggtcagct
     6421 tgtctttggt ctgaccttgg aactgcgggt cgaggacacg ggcagagagg acaaaggcgg
     6481 ttttgccgaa cacgtcgtcg ctttgtactt tcacgccgcg cggcaagagg ttgtgcagat
     6541 tgatgaaatt gttgacggcg ttgaacacgg cttgtttcaa gcctgcttca tgcgtgccgc
     6601 ccaaaggggt gggaatcagg ttgacgtagc tttcgttggc acacgagcct tcttccagcc
     6661 aagtcagggc aaacgccgct ccttcgccga tgctgaaatc gccgttgtgg ccgtctgaaa
     6721 tgtagttttc gcaagagaag ataggcacgg cttcctgcgc gtcggcaatc aggtcggtca
     6781 gatagctttt cagaccgtcg gggtaatgcc aggtttgggt gtgcgcttcg tcttcgcctt
     6841 tgaccggacg ggtcagggaa acgcgcacgc ccggcagcag cacggctttg gcgcgcagca
     6901 ggcgttcgag ttcggggatg ctgtaattcg ggctttcaaa atatttgccg tccggccaga
     6961 cgcgcacttc ggtgccgctg tctttgacgg cgcatttgcc cacttgtgcc aacggttcga
     7021 ccacgtcgcc gccggcaaac acgatacggt ggattttgcc ttcgcgtttg accgttacct
     7081 caaggcgggt ggaaagggcg ttggtcacgg atacgcccac gccgtgcagg ccgcctgaaa
     7141 aggcatacgc gctgccgccg tcttttttgt tgaacttgcc gcctgcgtgc agacgggtga
     7201 acacgagttc gactacgggt acgccttctt cagggtgcag accgacggga ataccgcggc
     7261 cgttgtcgcg tacggaaagc gaaccgtctt catgaatttg cacgtcgatt gccgtagcga
     7321 aaccgcccaa cgcctcgtcc gccgcgttgt cgatgacttc ttggcagata tgggtcgggc
     7381 tgtcggtgcg ggtgtacata ccgggacgtt ctttgaccgg ttccaaacct ttgaggacgg
     7441 tgatgctgga ttcgctgtat tggttgtttt tagccatagg aataatctga aagtaagaaa
     7501 aacaacgctt tcagacggcc tgaaagcgtt gcgttccgtt gttttaccgg ttgccggaaa
     7561 gttggcgggc ggcaaagtct tcataacttt ccataccgcg caggaagcgg gaagaaagtt
     7621 ctttcaatgc ccccaaataa acgtcgcgtt tgaaatcaat cacttcgtca acgggcgccc
     7681 aatattggtg ccaacgccag ccgtcaaatt cggggtggcg ggtggcgcgc aggttgacat
     7741 cgcaatcccg gccggtcagg cgcaggagat accaaatctg cttctgtccg cgataagagc
     7801 cgcgccattc gcggcgtacc cagttgttcg gcacgtcgta acgcagccag tcgcgcgtcc
     7861 gcccgacgat tttgacgtgt tgcggcagaa gtccgacttc ttcgtaaagc tcgcggtaca
     7921 tcgcggtttc ggggctttcg cccggcttga tgccgccttg cggaaactgc caagaatgtt
     7981 cgcgcacgcg cttgccccaa aagacttcgt tacgttcgtt aatcaggata ataccgacat
     8041 tggggcgata gccttccctg tccaacacgg tgtcgccctc cgttaaattc aatcgtacga
     8101 ttttcccaca aatcgggcgg ttttgacaaa tcagacggca tggttcaagc atgtcgaaac
     8161 acagacggac tcgggaaaat atcttaaatt tggtttacaa taatgtgttt caaattattc
     8221 gggaatcaga ccatgttaga tatccaattg ctccgcagca acaccgccgc tgtggcggaa
     8281 cgcttggcgg cgcgcggtta cgagtttgat gccgcgcgtt tcaatgcgct ggaagaacaa
     8341 cgcaaagccg tccaagtgaa aaccgaagaa ctacaagcct cgcgtaacag catttctaaa
     8401 caaatcggcg cactgaaagg tcagggcaaa cacgaagagg cgcaggcggc catggatcaa
     8461 gtcgcccaaa tcaaaaccga tttggaacag gctgccgccg atttggatgc cgttcaaaaa
     8521 gaattggacg catggttgtt gagcatacct aacctgccgc acgaaagcgt acctgccggt
     8581 aaagacgaaa ccgaaaacgt cgaagtccgc aaagtcggca ccccgcgcga atttgacttt
     8641 gaaatcaaag accatgtcga tttgggcgaa cctttgggtt tggattttga aggcggtgca
     8701 aaactctccg gcgcacgatt taccgtgatg cgcggacaaa tcgcccgtct gcaccgcgcc
     8761 ttggcacagt tcatgctgga tacgcacacg ctgcaacacg gctacaccga gcattacacg
     8821 ccttatatcg ttgacgatac gacgctgcaa ggtacgggcc aactaccaaa atttgcggaa
     8881 gatctgttcc acgttacccg tggcggcgac gaaaccaaaa ccacccaata cctgattccg
     8941 acagccgaag ttaccctgac caataccgtt gccgacagca ttatcccgtc cgaacaactg
     9001 ccgctgaagc tgaccgcgca ttcgccctgt ttccgcagcg aggcgggttc gtacggcaaa
     9061 gacacgcgcg gtctgattcg ccagcaccag ttcgacaaag tggaaatggt tcaaatcgtt
     9121 catcccgaaa aatcatacga aacgctggaa gaaatggtcg gccatgccga aaacatcctg
     9181 aaggctttgg aactgcccta ccgcgtgatt accctgtgta ccggcgacat gggcttcggc
     9241 gcggcaaaaa cgtatgactt ggaagtttgg gtgccggcgc aaaataccta ccgcgaaatc
     9301 tcaagctgct ccaactgcga agatttccaa gcccgccgcc tgaaggcgcg tttcaaagac
     9361 gaaaacggca aaaaccgctt ggtacatact ttgaacggct ccggtttggc agtcggcaga
     9421 accttggtcg ccgtgttgga aaaccatcaa aacgccgacg gcagcatcaa catccctgcc
     9481 gcactgcaac cgtatatggg cggcgttgcc aagttggaag tgaaataagc cttaaccctg
     9541 aacataaatg ccgtctgaaa cctggttcag acggcatttc ctttaaactt ttaaaacaca
     9601 tcaaccgtcg gcacgaaccg cattgccgca atcgccggtc tgtccgacct cgcggatatt
     9661 ggacagcgta acttccgaaa tattacccaa cgcctcttcc gtcaaaaatg cctgatggcc
     9721 ggtaaacagc acattatgac aagacgacag gcggcggaac acgtcgtcgg taatcacatc
     9781 gttggatttg tcttcaaaaa acaattcgcg ttcgttctcg tacacgtcca tacccaatgc
     9841 gccgattttc cggcgtttca acgcctcaat cgcggcagaa ctgtcaatca gcccgccacg
     9901 gctggtgttg ataatcatca cgccgtcttt cattttgtcg aacgccgctt cgttcagcat
     9961 atagtggttt tccggcgtgg aggggcaatg cagcgtgatg atgtccgacc gggcatacag
    10021 ctcgttcaac tccacatacc tgccgccgat tttttccgct tcggggttgc aaaacggatc
    10081 gtaagccagc aggttcatgc cgaaaccctt taaaatccgc atggttgcaa taccgatttt
    10141 ccccgtgccg ataacgcccg ccgttttgcc gtacatattg aaaccggtca gaccttccag
    10201 cgaaaaattc gcatcgcggg tacgctgata ggctttgtgg atacggcggt tcaacgtcag
    10261 catcagaccg accgtatgtt ccgcaaccga ttcgggcgaa taggcaggca cgcgcacgac
    10321 tttcaagccc aactcttcag ccgcctttaa atccacatta ttgaagccgg cacaacgcaa
    10381 cgccacagtt ttcacgccaa tttgcgccaa tttttccaac acgggccggc tgccgtcgtc
    10441 gtttacaaaa atacagaccg cttccgcgcc ttccgccatt ttcgccgttt tcgcatccag
    10501 cataaaatca aaaaactcca gctcgaagcc gaaatgccgg ttggcgcggg taaaatgttc
    10561 gcggtcatag cttttcgtac cgtaaatcgc aatcttcatc aatatgtcca gttgtcgcct
    10621 atggttgaga aacggcataa tacactgaat tcaaacaaat cagtagaata tggcagatta
    10681 aaattgattg catgcacggc atttccattt caaaacacaa aactcaatcg cccattgccg
    10741 ccagaagctc cgcctgatgc tcggcaatca gggcattggt gatttctgcc aagtcgccgt
    10801 ccatcacaaa atccagcttg tgcagggtaa ggttgatgcg gtggtcggtt accctgcctt
    10861 ggggatagtt gtaggtgcgg atgcgttcgc tgcggtcgcc gctgccgatg agggatttgc
    10921 gctcggcggc ttctttggct tgggcttcgc gtttttgcgc gtcgttcagg cgggcggcga
    10981 ggactttcat tgcctgcgct ttgttggcat gttggctgcg gccgtcttgg cattcgacca
    11041 ccatgccggt gggcaggtgg gtgatgcgga cggcggagtc ggttttgttg atgtgctgac
    11101 cgcccgcgcc ggatgcgcgg aaggtgtcga tgcgcaggtc ggctgggttc agttcgatgt
    11161 cttccagttc gtccgcttcg ggcatgacgg caacggtgca ggcggaggtg tggatgcggc
    11221 cttggctttc ggtggcgggg acgcgctgca cgcggtgtcc gcccgattca aatttcaaac
    11281 ggctgtacgc gcctaagccg ataatacggg cgataacttc tttatagccg cccaactcgc
    11341 tttcgttggc ggacacgatt tcaacctgcc agcggttgcg ttcggcgtag cggctgtaca
    11401 tacgcagcaa atcgccggca aacagcgcgg cttcgtcgcc gccggtaccg gcgcgtactt
    11461 cgataaagat gtttttgtcg tcgtcggcat ctttgggcag cagcaatttt tgcagttcgg
    11521 tatcgagcgc accgattttg gctttcgccg cttcgatttc ttcggcggca aagtctttca
    11581 tttcggggtc ggacaacatt tcttcggcat ccgccaagtc gctttgggca agccgatagt
    11641 tttggaacac ttcgacgacg ggggtcagtt cagcgtgttc gcgcgtgagc ttgcggtagt
    11701 tgtccatatc ggacgtggct tcgggttgcc ctaaaaggtg ggtaacttct tccaaccggt
    11761 cgccgagttg ttgcagtttt tctaagatag acggcttcat aattcttcca taacaaacgc
    11821 cgcctgaagt tcagacggca ttaaaacgcg gtcattataa tatattttct gaatattcaa
    11881 aaagataatc tagggacgga gcaggctgtc ggcattgcgt tccgttatcg cggcgatttc
    11941 cgccgcgccg gtaccgcgta tctgcgcggc aatttcggca atccgaagaa tattggcagg
    12001 cgtattgacg gtgtttttca acatgaacgg gctatccgtt tccaatacga aatccccgtc
    12061 gttcaaggct ttaagcgtat cgcgcacttt acgcgcgttc gggttgagca gcagcgaacc
    12121 gataccgatt ttgaaaccga gcccgaccaa cacccgcgct tcttcaatgc tgccggaaaa
    12181 ggcgtggacg atgccgccac aggaaaaacc tgtctgtctg acggcggcgg caatctcgtt
    12241 cgaggctttc aggttatgga taatcacgcg gcggcgcagg gtttgcgcga tggcaagctg
    12301 gcggctgaaa acttggattt gccgttcgcg ctgctgcggc gtttgggttt tatcgtaaaa
    12361 atccaagccg atttcgccga cccatgccat cggacgcgct ttcaatgctt gttccaaacg
    12421 ctggaaatcc ggttcggaaa cgtcgtctga aaaccaagga tggatgccga gcgcgatatg
    12481 gatattgccc caaagcggat tttcagacaa cctttgagcc aaatccgcca cgtcctgcca
    12541 atcctgcggc cgagtcgcgg gaacgataaa ccgccctacc cctgcctccc gcgcggcggt
    12601 caggatgtgc ggcaggtttt cgcgcagggc ggcatcggca aggtggcagt gggtgtcggt
    12661 gaagttcatt tcgattccgc aatgcggcag gttcacataa aaataaggca gggcggcaag
    12721 ggatgccgtg tacgggcggc gcataaggcg cggtcaacgc cccgtcattg ccatttcaat
    12781 ccgctataac ttcagtcttg ccaactcttt ttccacctct tccttacccc agccttgcga
    12841 tacggcgagg gtcatgagtg cggtggcggt ttcgaggttg catttgccgc cgttgatgat
    12901 gcccgagttg cggaacgcat tgccttgcgc gtaaacggcg gcggttttgc cttgtcggac
    12961 ttggctgatg ttgagcaaca attttccttg ccgcgtgaag tctcggacag cgcggataaa
    13021 gccttcgtct gcgggggtgt tgccgtgtcc gtagctttgc aggataaggg cttgggcgga
    13081 aagccgtccg agtccgtccg caagttcttg gacggcaaag ccagggataa gcgtgcggac
    13141 ggcgattttc gcctgcgggt cgggataatg gattttgagg ccgtctgaaa catctgccgc
    13201 gtcctgattc gggatgcgga cattgtgcca gccctgggtt tcgtcccact ccgccagtgc
    13261 gccgaaatgc ggattgtcga aaccggcggc ggtttcggtg ctgactttgc tgctgccgac
    13321 ggcgggatac agtttgccgt caaaggcaat aagcgtctgc ttcaatccga gtgggaatgc
    13381 ggcaacggca gtcgccagat tgcgcggcgc gtcgctgccc acggcgttgt acggccattg
    13441 ggagccggtc aggacgattg gcttgtccaa accctgcagg gcgagggcga acagattggc
    13501 ggtataggcg agcgtgtccg tgccgtgcag caccaaaacg ccgtcgtact gcggcagcgc
    13561 ggcggcaatg atgtccagcc aagcgcacca gttttgcagt tggacggcgg aagaatcaat
    13621 cagcggccca caaacgtgcc aatcgaaatc catgccgttt gaaaacggcg cgagtgcctg
    13681 cccgaccagc gcggtatcgg ggcgcaggcc ttcgctgctt tgcgtcatgc cgatggtgcc
    13741 gccggtataa aggacaaaaa ttttctgttt catggctcat cctgaaaatg caaagaccgc
    13801 ctgaaacgcg aaatgcgtcc ggacggtcga aaatctgatt tactgctgct tctgtgccgc
    13861 ttttttcgct gccttttccg ccttgcgttt ggcgcgtttt tcgctcaatt gtgcgcggta
    13921 aagctgataa tgtcggcgtt tgcgccacca gaaccaagcc agcgccgccg ccagcacgcc
    13981 caatgcgatg aagatgccgg attgcaggct gtgcattttc gccatcagcc aatcgatgtt
    14041 gtgcgcgccg tactcgccca agtaaatcca aacgggcacg gaaatcagcg cggcaagccc
    14101 gtccataatc agaaagcgca gatacgatac tttgcggctg atgccggcgg taacgaaaac
    14161 ggcagtccgc aaaccgggca ggaaacgagc gacaaataac acccagttgc cgtatttgtc
    14221 gaatttttcc tgaacctgtg cgtaacgttt cggcgtcatg atgcgcgcaa tcggtttgaa
    14281 cttgaggatt ttctgccccc aaattcgtcc ggcggcgaac atgatgccgt cgcccgccaa
    14341 cacgccgagc atgccgactg caaacataat atgcggattg gtataaccca tacccgaaat
    14401 cacgccgcct gttaccaagg tcaaatcctc gggaatcggc acgccgaaac cgcagatgac
    14461 caaaacgaaa aacacggccg cgtagccgta ttcgacaaaa aaggcttcca aaagggcaaa
    14521 catagcggat attccattgt cggagataaa aagtcagaac aagccgaaac attttctgcg
    14581 cgaaacggca ttctatcaaa gattatgccg tctgaaagcg gcagggggca gattcgcgcc
    14641 cccgccggac gttcagacgg catttaaaca ggaaaaccgc cgctgttgaa acggttttgc
    14701 ctgattttgc ctaaaggctg ccgatggcgg cggcaatgcg ttccgcacca tcgcgcgccc
    14761 aatccgcctg ccgcgcctcc accatcacac gcacgacggg ttcggttccc gaagcgcgca
    14821 acacgacacg ccctttgcct tcgagttctt tttccacttc cgccaacacg tcttttgagg
    14881 cttcctgcca gttttgccct ttgccgatac ggacattaat catcgtttgc ggatacggct
    14941 gccaatcggc gcaaacggtg gcgaggtctt ggttcagcgt ttgcagtgcc gccaaaactt
    15001 gcagcgcgga aataatgccg tcgccggtgt tgtgtttgtc catgcacaaa atatggccgc
    15061 tggcttcgcc gccgatgagc caaccgcgtt ggttcagctg ttccaacaca tagcggtcgc
    15121 cgactttggc gcggcagaaa tcgacgccct gctctttcag ggcgatttcc atcgccatat
    15181 tggtcatgac cgtgccgacc acgccgccga tgttgatacc ttctcgggcg cgggctttgg
    15241 caatgacgta aatcaggctg tcgccgtcgt aaaccttacc gtttttatcg accatcatca
    15301 ggcggtcgcc gtcgccatct aaggcgatac catagtcggc ttcatgctgt aaaacggcag
    15361 cctggagtgt cttggtataa gtcgcaccgc atttttcgtt gatgttgtag ccgttgggtt
    15421 cgtcgccgat gctgacgact tgtgcgccca gttcgtgaaa cactttgggt gcgacgctgt
    15481 aagccgcgcc gttggcggca tcgacgacca gcttcagccc gcgcaaatcg gaatgcgtag
    15541 ggaaggtgga tttgcaaaat tcgatatagc ggtcgtccgc gccgttgatg cggcgtgcgc
    15601 gaccgagacg ggcggacggt tgggttttca tttcgccgtc gattttggct tcgatttcca
    15661 actcgacttc atcggaaagt ttcacgccgc cttcggcaaa gaatttgatg ccgttgtcgg
    15721 aataggcgtt gtgcgacgcg gaaatcatca cgccggcgga caggcgcaac gcgcgggtca
    15781 gataagccac gccgggcgtg ggcagcggtc cggtctgtac cacattcaca cccgccgccg
    15841 taaaaccggc caccaaagcg gcttccagca tatagccgga aatgcgcgtg tctttgccga
    15901 tgaggacggt cggtttctgg tcggtgtcgt gctgcaccaa aacctgcccc gccgcatagc
    15961 cgagtttcaa tacaaaatcg ggcgtaatcg gaaactgccc gacttcgccg cgcacgccgt
    16021 ccgtgccgaa atattttttt gccatgtgtt gctccgagaa tgtggaccgt tgtccgagat
    16081 tatacagtca gtttgtgcct tgctgtctgc accgttgatg ccgtctgaaa ccaccccgcc
    16141 cttttcagac ggcatgaagt atgcgaaccg ctgtttacag attgatgccc aacgcttccc
    16201 acaccttcaa cgcgtccgcc gtcgccttca catcatgcac ccgcacgatt tgtgcgccgc
    16261 gcgccacgga agccagcgcc gccgccacgc tgccgtgtac gcgttccgcc gcatttgcct
    16321 cgccggtcag ctcgcctatc gtgcttttgc gcgatacgcc gatgagcagc ggaaaacctg
    16381 tttccgccat caattcgggc aaatgccgca tcagcgcgat attgtgttgc aagggtttgc
    16441 cgaagccgga gccgaagccg gggtcgagta tgatgcgttg cggtgcgatg cctgccgcga
    16501 tacattccgc tgagcgcgct ttcaaatacc gcgctacttc accgacaaca tcttgatatt
    16561 tcggattaat ctgcatggtt ttgggcaaac cctgcatgtg catcaggcaa atgcccgtgt
    16621 ccgcctgacg cgccagcaat tcgaccgcgc cctcgtcatt caacgccgcc acatcattaa
    16681 taatatcgac gccgccgagt gccaacgctt tttccataat caccgtgcgg cgcgtgtcca
    16741 aactgatggg aacgccccac cccgccactt ccgccaaaac aggctcaacc cgcgcccatt
    16801 cttcttcagg cgaaacataa tccgcacccg accgcgtcga ttcgccgccg atgtcgagaa
    16861 tgtccgcacc ctctttcaaa agccgttcgg cgtgcgccaa agctgtttgg gcattttgcg
    16921 aatacgcgcc gccgtcggaa aaagaatcgg gcgtgagatt cacgataccc atgattttcg
    16981 gtttgtccaa accgatttca aaccgtcctg cctgccaaac gtgtcgtgcc atctgaactc
    17041 ctcccaaaat aaaaaacaga ttatatgccg tctgaaaccg tcttgtgcgc ttcagacggc
    17101 accgctattc aggcggcaga cggcatgttg tccgaatgtc tgctccgcct ttgaatctgc
    17161 cggtatgcct gctatccgcc cgacttttca aaacaggttc cgacgattcc gcacgcgcct
    17221 gccgcctttg ccaagccgta caggatttcc tgcggcatat cgcggttcca taatcccgta
    17281 atattcgcaa tcacgggcag atggctgatt tggcggactt tcacgatgga ttcgacatcc
    17341 aaacggtagg gatggtcttt ggtatggctc aacacgcccg actcgcccag aatcaggtgg
    17401 cggttgccgc gcgagacaac atattctgcg gcattcaacc aatcttcggc agaatggtgc
    17461 ttgtctttac acagcaccaa gggaatgttc aggcttccgg cttcattcaa tacggcaaga
    17521 tcggacatca gcccgccgcc caaatacagg atgtccgccc ccgcattcaa agccgcttcg
    17581 acatggcgga cgttgcggac gcgcaccaat acgggtttcc ctgcgccatg cgccgatgcg
    17641 gtctgttccg ccaaccgtct gcaccgtcct cgcccttcat ccgcacttga agtgtcgtat
    17701 aagtttgccg aagtgaaaaa cggatctaga aacactgcat ccgcattgcg ccatactgac
    17761 ggttctgcgg cgatacagac ggtttccccg ccgccgaaag ccacgccttt ggcggcaacg
    17821 cggctgtctt ccgcccgatt ttcccgactg acggttttcc atgtatccaa aatgcggacg
    17881 gctttctcga cctccggcag cgtctgcacc tccctgacgc tcaaaaccct atcgtcgccg
    17941 attgcgccga tgacggtacg ctcgtcgccg tgagaaatgt gttctcgcag acctctgctg
    18001 cggataaagg cgacaacgcc ggcaatgtcc gcttcggcgg cacgcctgct catgacaata
    18061 atcatatttc ctcctgacac aagaaacggc ctgcccaaaa taggattttt gcaagccgtg
    18121 ttatactgtg gcgtgtttta cagattgttc gggctatgga tttattatcg gttttccaca
    18181 aataccgtct gaaatatgcg gtggccgtgc tgacgatact gcttttggcg gcagtcgggc
    18241 tgcacgcttc cgtatatcgc accttcacgc ctgaaaacat ccgcagccgc ctccaacaaa
    18301 gcattgcaca cacacaccgg aaaatctcgt ttgatgcgga cattcagcgc aggctcctgc
    18361 cccggccgac cgtcatcctg aaaaacctga ccattaccga acccggcggc gaccagactg
    18421 ccgtttccgt ccaagaaacc aaaatcggat tgagctggaa aaacctgtgg tcggatcaga
    18481 tacagattga aaaatgggtg gtttcgagtg cggaacttgc cctgacgcgc gacgggaaag
    18541 gtgtttggaa catccaagac ctgatcgaca gccaaaaacg ccaagcctca gtcaaccgca
    18601 ttatcgtcga aaacagcacc gtccgcctca atttcctgca ggaacagctt atcctgaagg
    18661 aaatcaacct caacctgcaa tcccccgatt cgtcggggca gccgtttgaa agttcgggca
    18721 tactggtttg gggaaagctg tccgtcccgt ggaaaagcag ggggctgttc ctttcagacg
    18781 gcatcggcac gcccaaaatc tcaccgttcc attttgaagc ttccacttcg ctggacggac
    18841 acggcattac catttccacc accggcagcc cttctgtccg cttcaacgcc ggcggagcgg
    18901 atgccgccgg cctcggcctg cgtgcagaca cttccttccg caacctccac ctgaccgccc
    18961 aaatccctac gctggcactc agaaataaca gcattaaaat tgaaaccgtc aacggcgcat
    19021 ttaccgccgg cggcgaatat gcccaatggg acggttcgtt caaactcgac aaagccaacc
    19081 ttcactccgg catcgccaac atcggcaacg ccgaaatctc cggcagcttc aaaacaccgc
    19141 gccaccagac caacttctcc ctcaattcgc cgctcgtatg gacggaaaac aaagggctgg
    19201 acgcgccgcg cctgtatgta tcgacccttc aggataccgt caaccgcctg ccgcaacccc
    19261 gtttcatcag ccggctcgac ggttcgctgt ccgtaccgaa tctgcaaaat tggaatgccg
    19321 aattaaacgg cacattcgac cgccaaaccg ttgccgcgaa attcagatac acacatgaag
    19381 acgcaccgca tctggaagcc gccgtcgcac tgcaaaaatt gaacctgacc ccctatcttg
    19441 acgacgtgcg gcaacaaaac ggcaaaatat ttcccgacac cctcgccaag ctgtccggcg
    19501 acatcgaggc gcacctgaaa atcggaaaag tccaacttcc cggcctgcaa ctggacgata
    19561 tggaaaccta cctccacgcc gacaaaggcc atatcgcgct cagccgtttc aagtcagggc
    19621 tttacggcgg ccataccgaa ggcggcatca gcatcgccaa cacccgtccc gccacttacc
    19681 gcctgcaaca gaatgcaagc aacatccaaa tccaaccgct gctgcaagac ctgttcggct
    19741 tccacagctt cagcggcaac ggcgatgcgg tcatcgacct gaccgcgagc ggcgaaaccc
    19801 gaaaagagct tatccgctcg cttcagggca gcctgtcgct gaatatttcc aacggcgcgt
    19861 ggcacggtat cgacatggac aatatcctga aaaacggcat ttcgggcaaa actgccgaca
    19921 atgccgcacc cagcacaccc ttccaccgat tcacgctcaa cagcgaaatt tcagacggca
    19981 tcagccgcca catcgatacc gaactcttct ccgacagcct ctatgttacc agcaacggct
    20041 ataccaatct ggatacgcag gaattgtctg aagatgtcct tatccgcaac gccgtccatc
    20101 cgaaaaacaa accgattccc ctgaaaatca ccggtacggt ggacaagccg tccattaccg
    20161 tcgattacgg caggctgacc ggcggcatca attcgcgcaa agagaaacag aaaatcctcg
    20221 aagacaccct gctggaacaa tggcagtggc tcaaacctaa agaaccgtaa acatcctgcg
    20281 tacacaaatg ccgtctgaaa cacccccacg cttcagacgg catgaccgta aaacctacaa
    20341 ccccaattcc tcccaaatcc catcaatctt agccgtaacc gcagggtctt ttttgatgac
    20401 gcgcccccat tcgcggtcgg tttctcccgg ccatttgttg gtcgcatcca aacccatttt
    20461 gccgccgagt ccgctgacgg ggctggcgaa gtcgagataa tcgatgggcg tgttttctac
    20521 caaaacagtg tcgcgcacgg ggtccatgcg cgtagtgacc gcccagatga cttctttcca
    20581 gtcgcgcaca tccacatcgt catccaccac gatgatgaat ttggtgtaca taaactggcg
    20641 caggaacgac cagcagccca tcatcacacg cttggcgtgt ccggcgtact gttttttcat
    20701 gctgaccacc gccatgcggt aggaacagcc ttcgggcggc aggtagaaat cggtgatttc
    20761 ggggaactgc ttttgcaaaa gcggtacgaa cacttcgttc aatgccacac ccaaaacggc
    20821 aggctcatcg ggcggtttgc ccgtgtaggt cgaatggtaa atcgggtttt cgcgcatggt
    20881 gatgcgttcg accgtaaaca cagggaaatg gtcctgctcg ttgtaatagc cggtgtggtc
    20941 gccgtatggg ccttccaacg cggtttcgtt cggatggatg acgccttcca acacgatttc
    21001 tgcgcgggca ggcacttgca aatcgttgcc gatacatttc accagctccg tccgcgaacc
    21061 gcgcagcagt ccggcaaact ggtattcgct caaggtatcg ggaacaggcg ttaccgcgcc
    21121 caaaatggtg gcggggtcgc agccgagtac gacggcgacg ggatacggcg tatcgggatt
    21181 gagtttgcgg aattcctgat aatccagcgc gccgccgcga tgcgacagcc agcgcataat
    21241 cagcttgttt ttgccgatta attgctggcg gtaaatgcct aagttttggc gttttttgtg
    21301 cggcccgcgc gttacggtca agccccacgt taccagcggc gcaacgtctt ccggccagca
    21361 atgttgaatc ggaagctgat acaaatcaac gttttcgcct tcccatacga tttcctgaca
    21421 cggcgcgttt ttcaccacgt tcggcgccat gctccaaata tctttcaaga gcggcagttt
    21481 ggaaaacgcg tctttgatgc ctttgggcgg ttcgggttct ttcagatacg ccagcgtctg
    21541 cccgatttcg cgcagcttgg acacgctgtc cgcgcccatg cccatcgcca cacgttcggg
    21601 cgtaccgaac aggtttgcca acacgggata atcgtagcgc gtaccgttgg gcttaaccgg
    21661 gtgttcaaac aacaacgccg gcccttcggc gcgcagcacg cggtcggcga tttcggtcat
    21721 ttccaaatgc ggggaaacgg ggtgcgcgac acgcttgagt ttgccctgct gctcgagcat
    21781 ggcgatgaag tcgcgcaggt ctttgtattt catgtttatc ctttttgtcc tttttatcct
    21841 gaacaatccg attcggatac cgcccctatc cttgcctgcg cttcggcata ttctatgccg
    21901 tgataaaagt cgcgtaccag cggatgtccg ccgctttgat aaagttgcag caaagggcgt
    21961 tggttgtcgg attcgtaagg gggcagccgg ttgcagatca taccgaaata aacggcgttt
    22021 tcagagttgc agaaaaatcg tcagcttgct gcacccacag caaaacccga tggattgtat
    22081 ttggtcgatg tgatttatcc acaaaagttt gatattccta aacgcccaat tggtccttta
    22141 tttttagagg atggtttatt aaatcgtact tgaaataaag cgtaatttaa tcctttaaat
    22201 aaccatatct gtaaaaaaga ctgcacttta aaaagtgcgg tcaattttaa gtgaatttta
    22261 tattaatttt gatattccat agttcactaa aaatccacca accactagcc ataaataagt
    22321 tagtattcct aaaacaaacg gttttaatcc tgcctttttg attgcgcttg cttgcgtcgt
    22381 taagccaagc gcagccattg atgaaattaa taagaaagaa tcgatttcaa cgaataattt
    22441 cacgagttct tttggtaata aatcaaaaga attaaaaatg gcaacaccaa taaaaagtac
    22501 agcaaaccaa ggaattgtaa ttttgtgtga tgtattttct gatactccat tactacgtgt
    22561 taataaccaa gaaagcatta ataaaaaggg ggcgagcatc atcactcgga tcattttgga
    22621 aatgacggca gtattcgcca cgataggatc aatattttcc ccaatcgcat aaacttgagc
    22681 cacttcgtgt acactagaac caacataaat accgaattga tgggcgttaa ttaaatgttg
    22741 tgaccacgtg tagaacaagg ggtaagtaaa aatagcaagc gtcccgaaaa tgaccactac
    22801 ggcaatcgcc actgaaactt tatgggattc tgctttggta acaggctctg ccgccatcac
    22861 tgctgccgca ccgcaaatgc tgcaacctgc cccagtgaga taaaccaatt gtttatccat
    22921 ttttagataa cgaatgccta aaagtgc
//
LOCUS       DAWFJR010000024        22270 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWFJR010000024 DAWFJR010000000
VERSION     DAWFJR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22270
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.024"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(120..1034)
                     /locus_tag="WGR04_000863"
     CDS             complement(120..1034)
                     /locus_tag="WGR04_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0794448.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WGR04_000864"
     CDS             1290..2759
                     /locus_tag="WGR04_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ0794449.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="WGR04_000865"
     CDS             3011..3382
                     /locus_tag="WGR04_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794450.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGNDVMQMLDSSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /locus_tag="WGR04_000866"
     CDS             3455..4864
                     /locus_tag="WGR04_000866"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ0794451.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPVR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALEKVGGKYGFTSGKSTHPDRLATIRQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDAAIPRPAALEGLETLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4911..5705
                     /locus_tag="WGR04_000867"
     CDS             4911..5705
                     /locus_tag="WGR04_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794452.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGSKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRAAGIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKSDAWLAGLEAIARVHKKRQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5893..6669
                     /locus_tag="WGR04_000868"
     CDS             5893..6669
                     /locus_tag="WGR04_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0794453.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(7336..9807)
                     /locus_tag="WGR04_000869"
     CDS             complement(7336..9807)
                     /locus_tag="WGR04_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ0794454.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRVGMDTPIAVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRISDGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMQSEKMPSEQ"
     gene            complement(9804..10982)
                     /gene="hflX"
                     /locus_tag="WGR04_000870"
     CDS             complement(9804..10982)
                     /gene="hflX"
                     /locus_tag="WGR04_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ0794455.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSTARLNGFQTAL
                     AEAVELVKAAGGNPVLVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(10987..12267)
                     /locus_tag="WGR04_000871"
     CDS             complement(10987..12267)
                     /locus_tag="WGR04_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ0794456.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGNGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            complement(12420..13145)
                     /locus_tag="WGR04_000872"
     CDS             complement(12420..13145)
                     /locus_tag="WGR04_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ0794457.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMNQINDCGRRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13218..13895)
                     /gene="radC"
                     /locus_tag="WGR04_000873"
     CDS             complement(13218..13895)
                     /gene="radC"
                     /locus_tag="WGR04_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ0794458.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGTGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVKELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            complement(14018..15367)
                     /gene="gshA"
                     /locus_tag="WGR04_000874"
     CDS             complement(14018..15367)
                     /gene="gshA"
                     /locus_tag="WGR04_000874"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ0794459.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWNAHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15627..17036
                     /gene="leuC"
                     /locus_tag="WGR04_000875"
     CDS             15627..17036
                     /gene="leuC"
                     /locus_tag="WGR04_000875"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ0794460.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRGLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17133..17387
                     /locus_tag="WGR04_000876"
     CDS             17133..17387
                     /locus_tag="WGR04_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794461.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17449..18090
                     /gene="leuD"
                     /locus_tag="WGR04_000877"
     CDS             17449..18090
                     /gene="leuD"
                     /locus_tag="WGR04_000877"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ0794462.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPSGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18264..18524
                     /gene="dcm"
                     /locus_tag="WGR04_000878"
     CDS             18264..18524
                     /gene="dcm"
                     /locus_tag="WGR04_000878"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0794463.1"
                     /translation="MQQIKFIDLFSEMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFA"
     gene            18570..19085
                     /gene="dcm"
                     /locus_tag="WGR04_000879"
     CDS             18570..19085
                     /gene="dcm"
                     /locus_tag="WGR04_000879"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0794464.1"
                     /translation="MARILKAKKPKGFILENVEGLVTHDRKDSTQKIGRTLTVILETL
                     EALGYYVSWKVLNAKDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPT
                     ESSPFIKKLLKKFPPSELYGKSVKDKRGGKNNIHSWDIELKGAVTEEEKQLLNILLKE
                     RRKKNGLQKSA"
     gene            19100..19534
                     /locus_tag="WGR04_000880"
     CDS             19100..19534
                     /locus_tag="WGR04_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0794465.1"
                     /translation="MPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRI
                     KDESLPKGYNIVSGKKSFEINKILDQNDVAPTLVAMNMEHLFVVDNGGLRTLTGKEGL
                     RLFGYPDDYSFDIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            19550..19711
                     /locus_tag="WGR04_000881"
     CDS             19550..19711
                     /locus_tag="WGR04_000881"
                     /inference="COORDINATES: protein motif:HMM:NF021105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ0794466.1"
                     /translation="MIKLTAQQIFDKLLDEEKILSANGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGG"
     gene            19712..20281
                     /locus_tag="WGR04_000882"
     CDS             19712..20281
                     /locus_tag="WGR04_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ0794467.1"
                     /translation="MRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPG
                     FDIADFKMYSDKIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWA
                     INLQVKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKK
                     KFEEAYKKHYNRSISIPRWHEIAHKYKKK"
     gene            20315..21385
                     /gene="leuB"
                     /locus_tag="WGR04_000883"
     CDS             20315..21385
                     /gene="leuB"
                     /locus_tag="WGR04_000883"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ0794468.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIEQGLDVSYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLNRPLRPERGLLAIRKDLNLFA
                     NLRPAILYKELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGENKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTND
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            21547..22110
                     /locus_tag="WGR04_000884"
     CDS             21547..22110
                     /locus_tag="WGR04_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ0794469.1"
                     /translation="MKKIIIAALAAAAIGTASAATYKVDEYHANARFSIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMAKTEVCGGDFSTSIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5375 a         5849 c         5785 g         5261 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgtgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcagaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta tcaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc ggcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttatggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcaaattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccggg ggcggtaaca cattcggcag cttagacggc ggcacaggca tgggcggcag
     3121 catcgtcaaa atggcggtcg ggagccaatg ccgtgcggaa ttggacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gagtgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacctgaacg gatgaccggc aacgatgtga tgcagatgct
     3301 ggattcgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatac cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcacccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgcgaaacc ttggacaaat ggcgcggttt
     3601 agcttatccc gaattggcgt ttgaaattat gcgcctgttt gttacggata ttccggagga
     3661 cgatttgcgc gacattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttgtcca acggcccgac
     3781 gctggcgttc aaagatatgg caatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcaaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaaaggtgt gaatgtattt atgctgtcgc ccgatggcaa
     3961 aatgagcgcg ttccaacgcg cgcaaatgta cagcctgcaa gacgggaata tccacaatat
     4021 cgccgtgaag ggaatgtttg acgactgtca ggatattgtg aaggcggtgc agaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac ggtcaattcg atcaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgcaggcta tttcaacgcc acttcaagca atgacgaaac
     4201 cgtcagcttc tgcgtaccga gcggcaactt cggcaacgtt tgcgcgggac atattgccaa
     4261 acagatgggc ctgcctgtcc gccgcctgat tgttgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggcg cataccgccc gcgcaacagc gcgcatactt atgttacctc
     4381 cagcccgtct atggacattt ccaaagcgtc caacttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctcaggaaa tcaatacgct gtgggcggaa gtagcggcgg gcaaaggctt
     4501 tgacctgcgg tttgctttag aaaaagtcgg tggcaaatac ggttttacct ccggcaaatc
     4561 cacccacccc gaccgcctcg ccaccatcag acaggtttat gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggggaaac
     4681 ggttgtgtgt ttggaaaccg cgttggcagc gaaattcgat gcgaccatac gcgaagccgt
     4741 cggcgatgcc gccattccgc gccccgccgc gctggaaggt ttggaaacat tgccgcagcg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatgttcc gtatgggcgg cattccgtcc gaacttaaga ttgaaagaaa atgaccttac
     4921 ctatgcagga aacccgtttt tccatactgc tggacgaatt ggcggcaaaa caggaggcag
     4981 ccatcgcccc ccacctgctt gccgacggga gcaaggtatg gatacgcaaa gcaggcaggc
     5041 acaatgcacg atggcgttac gcgctgctcg gtatggttgc ccgatatttg aaactggggg
     5101 tgttgaagcc ggtccccagc ctcggcggcg agcctgccat tgcaaccgaa tcaaaacgcc
     5161 tgtacgaatt gcgcgcggcg gggatagccg ttcccgaatt gctcgccgtc cggaaaaatg
     5221 cccttatgtt cggcaatttg gaaggcatcc cgctcgatac gcaaatccgc caagaagccg
     5281 aggctggaaa gtcggacgct tggcttgccg gtttggaagc cattgcacgc gtgcataaaa
     5341 aacggcagtt cctcagtcag gcgtttgcgc gaaatatgat gtcggacgga aaaaacatca
     5401 gctttttgga ttttgaagac gacccttccg aagtcctgac catagcgcaa tgccaagccc
     5461 gcgattggct ctgctacatc cattcgaccg cgctgatatt gaaaaacggc ggactgctgg
     5521 aagcggctgc ggaaaaatgg ggcggcgtat tgtcagatca gcctgccgaa atacaaaagc
     5581 tgattgccgc caccgtcaaa ccgatcctcc cgatacgcag gctggaacac ccccgctggg
     5641 gacgggacgc gctcaggctg gcagcctcga tttccctgat ttcccttgcc gatatgccgc
     5701 cgtaagccgc cgcccgcccc acgatgccgt ctgaagccct tcagacggca tataaccaac
     5761 gcgcatcaag cattgtcgca accatccgtt tcgtttagaa ttttggcttt accccgaatc
     5821 gggcagacat tttcactctg ccgcacatcc cgaacaagca ctatccattt cttttactga
     5881 caggaaaccc aaatggcagc attcaatacc caaaaagtat tgtccgtaca ccactggaca
     5941 gacgcatatt ttacctttac ctgcacccgc gacgaatcgt tgcgctttga aaacggacag
     6001 ttcgttatgg tcggattgat ggtggacggc aagccgctga tgcgtgcata cagcgtcgcc
     6061 tccgccaact gggaagaaca cctcgaattt ttcagcatta aagtccaaga cggcccgctg
     6121 accagccgcc tgcaacacct caaagtcggc gacgacgtgt taatcagcaa aaaaccgacc
     6181 ggaactctag ttgccggcga cctgaatcca ggcaaacacc tttacttgtt gagcaccggt
     6241 accggcatcg cccctttctt gagcatcacc aaagaccccg agatttacga gcaatttgaa
     6301 aaaatcatcc tcgtacacgg cgtgcgctac aaaaaagatt tggcgtacta cgaccgcttt
     6361 accaaagaat tgcccgaaca cgaatacctc ggcgacttgg ttaaagaaaa actgatttac
     6421 tacccgattg tttcccgaga agaattcgaa caccacggcc gcctaaccga cctgatggtg
     6481 agcggcaaac tgtttgaaga catcggcctg cccaaaatca atcctcaaga cgaccgcgcg
     6541 atgctgtgcg gcagccccgc gatgctgaaa gacacctgca aagttttgga cgatttcgga
     6601 ctaaccgttt ctccgaaaac aggcgtgcgc ggcgattacc tgattgagcg cgcgtttgtg
     6661 gatcaataag ctgcttgaaa atgccgtctg aacggcatca gcgataaaaa aacccgaatc
     6721 ctgccaatca ggattcgggg tttttatagt gggtaaaaat tagaaatgca catttccgtc
     6781 attccaacac aggtgggaat ccggacttac ctgtttcatt atcgtttgaa aataaaagag
     6841 aaaccaaaca tatagattcc cgcttgcgcg ggaatgacga cggtgttttg ccgtagccaa
     6901 agcagaaaaa catccctatt gtcatttcat catgatatga caataggatg ttaaaaatta
     6961 attatcatta tatgattgat atataagaaa aactgtccca ataagaaatg ttatgcagaa
     7021 aataaaatga aaaggatggt tttcattgaa aatggttgtt ctgccaccgt catacacaga
     7081 gccagtattt ggaaaaaaat gatccatatt gacatcatta ttccatttgt cgcataaatc
     7141 cactttttaa ttttttccac attaatataa tctcttattt aataaaatca aaataaaaat
     7201 caaatgccta acaattcgat ctcaaatttt gaacgccaac gtgtttgagc ggtaacaata
     7261 catactttaa gtttgcaatt cttcatcgtc ttatttttgt tttcatcaac tatacgtcta
     7321 aagcaacgcc gtccgtcatt gttcggacgg cattttttca gactgcattt tcccccgttt
     7381 gtgaaggcgc agggcgttgc ccaaaaccgc cagcgaactg aagctcatac ccagtgcggc
     7441 tatccacggt tggacatagc ccaaaacggc aagcggtacg gcaatgatat tgtacgcgcc
     7501 cgcccatatc aggttttgcc ggataatatg gcgggtgcgc cgcgcctgat cgagcaggtg
     7561 ggcgacggta cgcaaatctt cgttcaataa cacaatgtcc gcgccgtccc tcgcaatatc
     7621 cgtcccgccc gctgcggcgg cggatacgtc tgcctgcgcc aaaacgggcg cgtcgttgat
     7681 gccgtcgcct atcatcagca cttttttccc ttctttttgc aaggctttga cgtattccag
     7741 tttgtcctcg ggcatggctt gggcgcggta gtgcgcgaca cccagggcgc gtgcggtttc
     7801 ggcaacggcg gtttcgcggt cgccgctgag gatgtgcagg gtcaggtttt tgcctgccaa
     7861 ctgccgcacc gcctccgccg cgctgtcttt caaggggtcg gtcaggtaga acacggcttg
     7921 gaaaccgctt tgactgccga ggtaaaccgc gctgccgccg ccttctgttt gcggttcttt
     7981 acctgaaatt tcggcgacat aggatgccct gcccaatgcc caaacctgtg tttcgccgtt
     8041 gacggtcagt tgcgcgccca cgccttcgcc gatgcggttg aggcgttgtt taatagcgat
     8101 gtcggggacg ctgccgtctg aaatgcggca gttgaggatg gcgcgggcaa ggggatgttc
     8161 ggactgttgt tctaaagcct gcgccaccgc gagaacaaag gcttcgtctg tgcctctcaa
     8221 caatgagata cggcggacgg cgggtttgcc ttgggtcagc gtgccggttt tgtcgaagat
     8281 gatgtcggtg gtttgggcga gggtttcgat tgcctgcttt ccgccgatta aaataccttc
     8341 gcgcgccagc gtaccggtag aagctgccag cgcggtcggc gtggcaagcg ataaggcgca
     8401 ggggcaggta atgaccagca gggcgacggt aatccacaat gcggtgtgcg cgtcggcgta
     8461 cagcgtccag ccgatgaaga cggggacggc aagcaggagt tcgccgaata tgaaagacga
     8521 ggcgtattgt tccgccaact cggcagtgcg cggtttttgc gctaaggcgc ggtcgagcag
     8581 gcggacgatg tgcgacagtc gcgtgccgcc gccggtgcgg tcggtgcgta taatcagggg
     8641 gctttgcgtg ttgagtgtgc cggcggttac tttttcagac ggcattttgg cgacgggcag
     8701 gctctcgccg gtcagcatag attcgttgac ggcactgctt ccttccagca ccgtgccgtc
     8761 aacggggatg gtttcgcccg gtttgaccag cacgatatcg cccgccttca atttgacgac
     8821 agctgcctcg caggtttcct gcgtatcggg gtaatcgggc atatgatggc aaaacgcagg
     8881 aatcagcttc accagcctct cggcggcatc gcctgcctta cggcgggcaa tgtgttccat
     8941 aaagcgtccg cccagcagga aaaacagcag catcgcgatg gattcgaaat acatcccctg
     9001 ccccgcattt gtcgcaaggc tgtaaacgcc ggcgataaag gtcatgatga tggcgacggc
     9061 aatcggcgta tccatgccga cgcggcggtt tttcaagtcg cgcagcgcgc cttgataaaa
     9121 cgggacggcg caatagaata cgacgggcag caccattaaa aagccgcccc aatggaggat
     9181 ttgcaggaaa tcgggttcga tgtcgccgcc gtaaaggtag gtcggcagcg cgaacatcat
     9241 cgtctgcatc atccccagcc cggcaacggc gaggcggacg atgtattgtt tgcgttcttt
     9301 ttggttggcg gcttcgattt tttgcgcgtc atagggtgcg gcggtgtagc ctatctgcct
     9361 gattttcaac agaatgtcgg aaaggcggat tttgccgtcg tcccagacga cgcggcagcg
     9421 gtgcgtgctg taattgaggt cgatgcggac gatgccgtct gtacgcaaaa gctgctgttc
     9481 gatcagccag acgcaggcgg cgcaggtgat gccgccgagc attaaaaccg cctcgcgcgt
     9541 accgccgtgg gtttccacaa agtcggactg gacttcgggc aggtcgtaca ggcggatttg
     9601 gtcgaggatt tcttggggcg gcagctcggt tttttgcgcg tcggcggtgc gttgtttgta
     9661 ataactgccc aagcccgcgt caataatgct ttgtgcgacc gcctgacagc cggcgcagca
     9721 ggtttcgcgg tcttcgtttt cgtaacggac agtcaggtgg aggtgttcgg gaacatccag
     9781 accgcagtgg aaacaggttt ttttcatggc atttcggttt cgtctgtgtt tggtgcggcg
     9841 gcacaagact cggcaatggc ttcgcgcagt gcgtctatac cggtattttc agcaacggaa
     9901 atgcggacgg cggcaatttt tcccgcagcg tcgcgccata tgcccgtgtt ttgttcttca
     9961 gacggcagca ggtcggtttt gttgtacacc ttgatgcacg gaatatcgcc ggcatggatt
    10021 tcttgcagta cgttttccac gtcttcaatc tgctgtccgc tgttcggagc ggcggcatcg
    10081 acgacgtgca gcagcacatc ggcttgcgcg gtttcttcca gcgtggcgga aaaggcggaa
    10141 atcagtttgt gcggcagatc gctgacgaat ccgacggtat cggtcaggat aatgctgcat
    10201 tcgggactga tgtacagccg ccgcgccgtc gtgtcgagtg tggcgaaaag ctggtctttc
    10261 gcatatatgc ccgacttggt cagccggttg aacaggctgg atttgccgac attggtatag
    10321 ccgaccagcg caaacgtttt gattgtgccc gattcgcggg acttgcggcg cagggcgcgc
    10381 tgttttttga ggttggcaag ctgttttttc aaggcgttga tccgatgggc gatcaatcgg
    10441 cggtcggttt ccagtttggt ttcgcccggc cctttcatgc cgataccgcc gcgctggctc
    10501 tgcagatggc cgtaaccgcg tatcaagcgt cccgccaaat ggctcaattg tgccaactca
    10561 acttgcagcc tgccttcctg cgtgcgggcg cggcgggcga aaatcgccaa aatcaaccct
    10621 accctgtcca gcacgcggca tttcagttct ttttcaaggt tgcgttcctg cgtgggcgta
    10681 agttcatggt tgaatacgac caaatcgatg ccgtctgcgg caactgcttc cgacagctcc
    10741 gccgccttgc ccgtgccgac aaacagcgcg gtgtgcggac ggtcgcgttt ggcggtttcc
    10801 acaaggacgg gattgccgcc cgccgccttg accagctcga cggcttccgc caatgccgtc
    10861 tgaaagccgt tcagacgggc ggtactgctg cccgtaccgt ctttgtccaa cattacgccc
    10921 accagcatga cgcgttcggg ttgcgcttgg gtactgccgt tgcggcctgt tctgcctgtc
    10981 atgtattcaa tcctgtaaat aaagggttac gcctcgttcc gtttctcctt ccggtgcaag
    11041 cgtgatgcgg ctgcccgcca ttccgtcaac gctgaagacg atgccgcccg cctcgtgcct
    11101 tcctatggac aggcggctgc cgggtcgctg ccggtaggta taacgggtac acgcttccaa
    11161 acaccagcgt ccgcgccagt ggatgaaata tttttcgcct tttctaaccg caagtgtctc
    11221 gccgccgttt acctcaaaat aagggtaatc gggttttcgg gttctaaact gccatttcgc
    11281 ctgcgcgcgc cgtccgttgc ggacatagtc gaataccgcc gtataaagcg taccgtattc
    11341 caaaggcttg agcgggaaaa gcgcgaattg gtacgcggtc agcctgccgt tggggtcgtt
    11401 gccggcggtt aaaaccctga cggggcggat ttcgttttta ccctgataca gcttgaaact
    11461 tttcatcgta attttgcctg ccgcctcgga aaaatcaatg ctggcaggat tgcccgtgat
    11521 ttcatattcc ggcacggggt ctggacgctc gccgtggaaa taaggcagtg cgccgttgcc
    11581 gacgggatag gctgtataga gcagctcctg tgcgggcatg gcttcgtcgg tgtacacgac
    11641 cgcaccgtta tggcaggcgt tgcggtaata tttccgtcct gcttccggct gatttctgcc
    11701 ttgggcgcaa tgccgctcaa acctgccgtt gccctgattg aatacgagaa cggttttacc
    11761 gttttcgcgc acaaatgccg ctcctgcctc atccgtatgg cggtcaagta gggaaaggcg
    11821 gtggtagatt gcgctcatta atccgtccac ttggcgttgc tgcgtgcgga tgtcgctgtc
    11881 ggacgattcg gcggcttcct cttccgtgct gatgttttca tgcacgccgt tgtagagata
    11941 cccggcaagg cgtgtgcgtt cggtcagctt ttgtgcggtg tagtgcggat tgtcgggatg
    12001 gtgttcgccg tgtccgtctt cgggattgag cgtgagatag cgtgcgtgcc tgcgggcgga
    12061 attttccaaa accggcgcgt gtgccagctt gtgcaaaccg atttgggcgc ggatgcggtt
    12121 gaggtagtgc agcgcatcgc cgccgtccct gatttgcggt gcggacggat agacaagttc
    12181 gcccgcgggc agggattggt tttgggtatg atagaaaacg ccggccgccg aacccaacag
    12241 gagcagccga ataaaaaggg atttcataat gccgtcctcc cgacgcggca acggggtgtt
    12301 ttatcaaaac ggcaaacttg ccaatgcggt ttccgattgc agattgccaa accggcaaac
    12361 ctttccccgt gccgatgccg aacccgatgc cgtctgaact ttcagacggc atcgggtgtt
    12421 tatttgtcca cccggatggg tgcaggttcg tattgtgtcg attcgtcgcc gtaatacagc
    12481 acgccgagtt tgatggggat gcgtccctgc gatttgcggt gggcattgga atccctcagg
    12541 gaataggcgc aaccgcaata ttcctgctgg tagaagtttt cgcgtttgct gatttcaatc
    12601 atgcgcgcgc tgccgccgcc tttgcgccag ttgaaatccc aatacaccac atcatcgtaa
    12661 ggcgcggcgg cgcggcggcc acagtcgtta atctggttca tgtttttcca acgggaaatg
    12721 cccagcgagc tggtgaagac ggggaaccca tgttcatgcg cgtattgcgc ggctttttca
    12781 aaacgcatat cgaaacacat cgtacagcgg atgccgcgtt ccggctcaaa ctccatacct
    12841 ttggctttgg caaaccattc tttgcggtcg ttttcgtaat cgtcgtcttt gtcgatgaag
    12901 gggatgccga acttttccgc aaagcgcacg ttttcctctt ttctgagcat atactctttg
    12961 tgcggatgga tattgggatt gtaaaaataa atggtgtagt cgatgccgga ggcaagcatc
    13021 gcctccatca cttcgccgct gcacggggcg cagcaggaat gcagcaagac ttttttatgt
    13081 ccgccgggcg ggacgagtat ggggcggtca atgtcggtaa cggtaggttt gttttgtgtt
    13141 tccattaact taacaatatc tgtcgaatac ggttgaagcg gctattttat cagagccgcc
    13201 gcatgtaaaa cagggtgtca gggcatcagc ccgagctgcc tgaacgaacg gacggtttgc
    13261 gccgttacga taaaatggtc gagcagcgac acatcgacca gcgacattgc ctgtgccagc
    13321 cgccttgtga acatgatgtc ttcctgcgaa ggttcgggcg agccgcccgg atggttgtgc
    13381 gcgataatca ggctgtcggc atactcgtcc aaagccagtt tgacgatttc gcggatgtaa
    13441 atcgtgtttt ccgcaaccgt accgcgcgac agctctttga ccgcaatcag ttggttttgg
    13501 cggttcagca gcagcgcgac gctgacttcg actttttcct gccccaaatg aaagcgtaaa
    13561 taatcggcca ccgtatccgg atcggacagg acgatgctct cctgcaattc ttccgccaat
    13621 atccgccgcc cgatttccct gaccacggca aactgtgtga aacttgccgt ccccgtccct
    13681 ttgtatgccg acagtttgcc gacctccgcg ctcatcagcc tccccaaact gccgaactcc
    13741 tgcagcaaat aacgcgccaa atcgaccgca ctcattccgc gcgtgccgac gcgcagcagg
    13801 attgccaaaa gttcggcatc actcaacgcc gccgccccgc gttccaacag cttttccctg
    13861 ggtctttcgc cttccggcca ttgcttgatg ctcatgtttt ccctttttta aatagtctga
    13921 gatgccgtct gaaacagatg ttcccacacg ccaaatatcc gaaatgccgt ctgaaccgga
    13981 tttccgcttc agacggcatc tgtatttcaa aacagtctta cgccgtttct tccaactcga
    14041 ttgaagccgc cagaagcgac aggcgtgcca ttacgccgta aacgtagagg cggttgtgtt
    14101 cgttgtccac gtcgcagtct ttttcagagc gcggcatacc cagcgagtcc cattgttcga
    14161 atacgcgctt gcaggcttcg ggcgcttctt gggaattatc gccgttaccg gtaggaatgc
    14221 tgttagacag cggaacaaac accataccgc cggcgttgag gttttcgtcc gcaccgcgcc
    14281 cttcgtgtac gcggaaaaag ccgccgatga cgaagcggtc catcatatac acgacgggtt
    14341 cgcacaccgc gccgtttaag gtttcataag tataaatccc ttcttggaca atcacttcgc
    14401 tgacttccaa accttctttg acttttgcca ttttgttgcg gtttttacgg ttcaagccgc
    14461 gcacttcatc cgcagatttg acgctcatca cgcccatgcc gtaagtgccg gcatcggctt
    14521 tgacgatgac gaaaggtttg tcggtaatgc ccgattcgtc gtatttggct tgaattttcg
    14581 ccagcacacg ttctaccgct tccgccaacg cgtcttcgcc ttcacgccct tggaagtcca
    14641 aaccgccgat tttttcaaaa taagggttaa tttgccattc gtcgatgccg attaacttgg
    14701 caaattcggc ggtaacttgg ttgtacgcgc cgaaatgatt tgttttgcgg cgcgtcgtcc
    14761 aaccgccgtg caacggcggc aaaacggttt ggctgatgcc tttgaggatg tcgggaatgc
    14821 cggcagacaa atcgttgttc aacaaaacca cgcaaggcga aaagccgtct gcaagatgga
    14881 cgcggtcgcg ggtacgcaat aaaggttcca acaggatttt gtcgcccaat gcagtttcaa
    14941 attcggtcgg ttcggttact tccggattca ggctgcccaa gcgcacttca taccctgccg
    15001 aacgcaaaat ctcgccgagg gcgtaaatgt tttgcaggta aaacgtattg cgcgtgtggt
    15061 tttcgggaat aatcaatacg gatttcgccg tttcgcaggc gcgttgcacc gcatcttgcg
    15121 cggcaaccgc cgccagcggg ataaagttgg gattcagatt attgaagccg ccggggaaca
    15181 aattcatatc gatagacgaa attttgtaac cggcattgcg tatatcgacc gaaccgtaaa
    15241 acggcgggcg gtgcgcgttc cactgcgtgc ggaaccacgc ctcgattttg gcgtgattgg
    15301 acaggatttt tgcctcaaac gcctgaagtt gcgccgaatg ttcgggcgac atcaccggta
    15361 atttcatctt tcgtctgcct ctgtgtgttt tttgacaaga tgcagatgat aatgcctttg
    15421 aaagaatgac gacaagtatt ctttcgtatc gaatcatgct ttagatataa ctgtttactg
    15481 aaatggacaa tatgtctaat tttttcgtta aaattgtttg caaaataaca ccggctcaaa
    15541 aattagacaa aatttgttgc gcggtataaa gaatacgtct aaaatccgcc caatccgcat
    15601 taccatttat ccaaaagaac aacatcatga cagcacaaac cctctacgac aaactttgga
    15661 acagccacgt cgtccgcgaa gaagaagacg gcaccgtcct gctctacatc gaccgccatt
    15721 tggtgcacga agttaccagc cctcaggcat ttgaaggctt gaaaatggcg gggcgcaagc
    15781 tgtggcgcat cgacagcgtc gtctccaccg ccgaccacaa caccccgacc ggcgattggg
    15841 acaaaggcat ccaagacccg atttccaagc tgcaagtcga tactttggac aaaaacatta
    15901 aagagtttgg cgcactcgcc tattttccgt ttatggacaa aggtcagggc atcgtacacg
    15961 ttatgggccc cgaacaaggc gcgaccctgc ccggtatgac cgtcgtctgc ggcgactcgc
    16021 acacttccac ccacggcgca ttcggcgcac tggcgcacgg catcggcact tccgaagtcg
    16081 agcacaccat ggcgacccaa tgtattaccg cgaaaaaatc caaatccatg ctgattgccg
    16141 ttgacggcaa attaaaagcg ggcgttaccg ccaaagacgt ggcactttac atcatcgggc
    16201 aaatcggcac ggcgggcggc acgggttacg ccgtcgagtt cggcggcgaa gccattcgcg
    16261 gcctttcgat ggaaggcagg atgaccctgt gcaatatggc gatcgaagcc ggtgcgcgct
    16321 caggcatggt tgccgtcgac caaaccacca tcgactacgt aaaagataaa cccttcgcac
    16381 ccgaaggcga agcgtgggac aaagccgtcg agtactggcg tacgctggtg tctgacgaag
    16441 gtgcggtatt cgacaaagaa taccgtttca acgccgaaga catcgaaccg caagtcactt
    16501 ggggtacctc gcctgaaatg gttttagaca tcagcagcaa agtgccgaat cctgccgaag
    16561 aaaccgatcc ggtcaaacgc agcggtatgg aacgcgccct tgaatacatg ggcttggaag
    16621 ccggtacgcc attaaacgaa atccccgtcg acatcgtatt catcggctct tgcaccaaca
    16681 gccgcatcga agacttgcgc gaagccgccg ccatcgccaa agaccgcaaa aaagccgcca
    16741 acgtacagcg cgtgttgatc gtccccggct ccggtttggt taaagaacaa gccgaaaaag
    16801 aaggcttgga caaaattttc atcgaagccg gttttgaatg gcgcgaaccg ggctgctcta
    16861 tgtgcctcgc catgaatgcc gaccgcctga ccccgggtca acgttgtgcc tctacttcca
    16921 accgtaactt tgaaggccgt caaggcaacg gcggacgtac ccacctcgtc agccctgcca
    16981 tggcagccgc agccgcagtg accggtcgct ttaccgacat ccgcatgatg gcgtaagtat
    17041 tttcaggcgg tctttaagaa tttgacgcgg ccgtctgaaa tatgtcccgt aaggcagaaa
    17101 cacctttaat attcaaacca aggagtttaa ccatgaacaa acttttcatt accgccctgt
    17161 ccgccctcgc cttgtccgcc tgcgccggca cttgggaggg cgcgaaacaa gacaccgccc
    17221 gcaaccttga caaaacacag gccgccgccg aacgcgccgc cgaacaaaca ggcaacgccg
    17281 tcgaaaaagg ctgggacaaa accaaagaag ccgtcaaaaa aggcggcaat gccgtcggac
    17341 gcggcatttc ccatctcggc ggaaaaatcg aaaacgccac cgaataaccg attcgcgatg
    17401 ccgtctgaaa ccgcttcaga cggcatcgtc agcacaagac gcaaacccat gaaagccttt
    17461 accaaaatta ccgccatcgt cgccccgctc gaccgcagca acgtcgatac cgacgccatc
    17521 atccccaaac aatttttaaa atccatcaaa cgcagcggct tcggccccaa tgcctttgac
    17581 gaatggcgtt acctcgacca cggcgaacca ggcatggaca acagcaaacg cccgttgaac
    17641 cctgatttct cgctgaacca gccgcgttac caaggcgcgc aaatcctgtt gacgcgtaaa
    17701 aacttcggtt gcggctcttc acgcgaacac gccccttggg cattggacga ctacggcttc
    17761 cgcgccgtca tcgcccccag cttcgccgac atcttcttta acaactgcta caaaaacggc
    17821 cttttaccta tcgtgttgac cgaagaacaa gtcgatcggc ttttcaaaga agttgaagcc
    17881 aacgaaggct atcagctctc catcgacctt gccgaacaaa ccctgaccac accaagcggc
    17941 gaaacattca cattcgacat taccgaacac cgcaaacact gcctcttaaa cggcttggat
    18001 gaaatcggtc tgaccctgca acacgccgac gaaattcacg ccttcgaaga aaaacgccgc
    18061 caaagccagc cttggctgtt taacggttaa gcctatgccg tctgaaggcc gttcagacgg
    18121 cattcctgat aacatataat tgttgcggct aatgtaaaca gaggggcgat aaattgcttt
    18181 catcttttaa gcgggcttag cgacactcta aattaatgca ataatgcaac tctccatttc
    18241 aaataaattt ttagattttt aatatgcaac aaattaaatt tattgactta ttttccgaaa
    18301 tgagtggcat caggaaagga ttcgaacaag cctgtcggaa acagtcggtt gcttgcgaat
    18361 gtgtttttac ttcagaaatc aaaccggcag ctttggaagt attgaagcaa aactaccctg
    18421 atgaagtgcc gtatggagat ataacgaaaa ttgaaacagg ggatattccc gattttgaca
    18481 tcctgttggc aggcttccct tgtcaggctt tttctttcgc ctgaaaaaga ttaggctttg
    18541 aagatacacg gggaacgctt ttctttgatg tggcaaggat tttaaaggca aaaaaaccaa
    18601 aaggttttat tttggaaaat gtggaaggat tggtgacaca cgatagaaaa gattcgacac
    18661 aaaaaatagg gcgcaccctg accgttattt tggaaacctt ggaagcattg ggctactatg
    18721 tttcttggaa agttttaaat gcaaaagatt tcggcattcc ccaaaacagg aagcgcattt
    18781 atctgacagg cagtttgaaa tccaaacccg acttatcttt cgaaacaagc ccaagtccga
    18841 aattaaaaaa tattttggaa tcgggactgc ctaccgaaag cagccctttc atcaaaaaat
    18901 tgctaaaaaa atttcccccg tccgaactgt acggaaaatc agtaaaagac aaacggggag
    18961 ggaaaaacaa tattcacagt tgggatattg aattaaaagg cgcagtaacc gaagaagaga
    19021 agcaattgtt aaatatcctt ctaaaagaac ggaggaaaaa aaatgggctt cagaaatcgg
    19081 catagattgg atggatggga tgcctttgac aaaagcgcaa atttcaactt tctataaaca
    19141 ccccgatctc caaaatattt tggacagcct gacagataaa ggctatttgg ttttagagca
    19201 tccgaaacaa aaaattggcg ggcaaagaat caaagatgaa tccctgccca aaggctacaa
    19261 tattgtttca ggcaaaaaat cttttgaaat caataaaata ttagatcaaa acgatgttgc
    19321 gccaaccttg gttgcaatga atatggagca ccttttcgtc gttgacaacg gcggtttgcg
    19381 tacacttacc ggaaaagaag ggttgcgctt attcggctat ccggacgatt attcgtttga
    19441 tattcccaaa aaagacagat gcgatttatt gggtaatacc gttgccgtcc ctgtgattaa
    19501 ggcggtatct gaaagacttc tgcatacttt ataaaccgac agggataaaa tgataaaact
    19561 tactgcacaa caaatatttg ataagttgct ggatgaagaa aaaatcttat cggccaatgg
    19621 tcaaattaga tttttcttag gcgatgtgga tattatcgtc aaacaaaaag atgttgtcgg
    19681 caacatcatt caggaatggc ttggcggatg attgagaaaa agggaaattg aatttgatgt
    19741 ttcaaccaat acccaaatgc ctcccgattt ctttttaaat aaaaaagacc gcagcagaga
    19801 attgttggaa gtaaaggcgt tcaaccgaaa tgccggcccg ggttttgata ttgcagattt
    19861 taaaatgtat tctgataaaa tcattcataa gccctatatg ctggatgtag actatttaat
    19921 attcggttac gatatggacg acaacggcaa tgtaaccatc aaggatttat ggcttaaaaa
    19981 agtatggcaa attacgagaa gcatggatgg gtgggcaatt aatcttcaag tcaaaaaagg
    20041 cgtggtgcat aaaatccgcc cgggtgtttg gtacagcata aataaaaaga atatgcctat
    20101 gtttgaatgc ttggaagatt ttgtttccgc aattgaagaa accgtttatc aaaacccggc
    20161 aacacggcat aacgcatcct tatggaaaaa gaaatttgag gaagcctata aaaaacacta
    20221 caaccgatcg atttccatac ctcgttggca cgaaattgca cacaaataca aaaagaaata
    20281 acccttcaac catcaaccca taaggaaact tgaaatgacc aaacatatcg ccatcctccg
    20341 aggtgacggc atcggtcccg aaatcgtcgc cgaaaccgtc cgcgtactcg acaaacttat
    20401 cgaacaaggt ttggatgtca gctacgaata cgcacctttg ggcggcgaag cctatgacga
    20461 atacggccat ccttatccag aattcacgca aaacctttgc cgtaaagccg atgcggtcct
    20521 gcttggtgca gtcggttctc ctcaatacga caatctcaac cgtccgctgc gccctgaacg
    20581 cggcttattg gccatccgta aagacttgaa tctttttgcc aatttgcgcc ctgccatttt
    20641 gtataaagag ctggccaatg cttctacttt gaaaccagaa atcgtagccg gcctcgacat
    20701 ccttatcgta cgcgaactga ccggcgatat ttactttggc gaaccgcgcg gcatccgtgt
    20761 tttggaaaac ggcgaacgcg aaggctacaa caccatgaaa tacagtgaaa gcgaaatccg
    20821 ccgtattgcc cacgttgcct tccaatccgc ccaaaaacgc agcaaaaaag tctgctccgt
    20881 aggcaaagcc aacgttttgg aaaccaccga actttggcgc gaaatctttg aagaaatcgg
    20941 caaacaatac cctgatgtcg agctttccca tatgtacgtt gataacgcag ccatgcagct
    21001 cgtgcgcgcg cccaaacagt ttgacgtgat tgccaccggc aacatcttcg gcgacatcct
    21061 ctccgacgaa gcctccatgc tgaccggctc catcggtatg ctgccttccg cttctttgga
    21121 cgaaaacggc aaaggcctgt acgaaccgtc tcacggctcc gctcccgaca tcgccggaga
    21181 aaacaaagcc aacccgctgg ccaccatcct ctcgcttgcc atgctgctgc gttacagcct
    21241 gaacgacgaa gcgcgcgcgc aacaagttga aaacgccgta caaaaagtgc tgcaacaagg
    21301 cttgcgtacc aacgacattt acgaagaagg cacgaaactc gtttcctgtt ccgaaatggg
    21361 cgacgcggta ctcgccgcct tgtaaaaaac aaagcgcgga taacaccgcg ctttttcatc
    21421 cgcccttttc tgcccgcccc gttcgtcaac cgcctgcaaa cccaccgttt accggcagga
    21481 aatgccataa ccccccgcct atgttaggat aaacgccttt accggcccac acaaaggatt
    21541 atcatcatga aaaaaatcat catcgccgcg ctcgcagcag ccgccatcgg cactgcctcc
    21601 gccgccacct acaaagtgga cgaatatcac gccaacgccc gtttctctat cgaccatttc
    21661 aacaccagca ccaacgtcgg cggtttttac ggtctgaccg gttccgttga gttcgaccaa
    21721 gcaaaacgcg acggtaaaat cgacatcacc atccccgttg ccaacctgca aagcggttcg
    21781 caacacttta ccgaccacct gaaatcagcc gacatcttcg atgccgccca atatccggac
    21841 atccgcttcg tttccaccaa attcaacttc aacggcaaaa aactggtttc cgttgacggc
    21901 aacctgacca tgcacggcaa aaccgccccc gtcaaactca aagccgaaaa attcaactgc
    21961 taccaaagcc cgatggcgaa aaccgaagtt tgtggcggcg acttcagcac cagcatcgac
    22021 cgcaccaaat ggggcgtgga ctacctcgtt aacgttggta tgaccaaaag cgtccgcatc
    22081 gacatccaaa tcgaggcagc caaacaataa atcttgatgc gaatcagcac aaatgccgtc
    22141 tgaacgtcct tcaaacggca tttgtgccgt cttcatcaaa ccgccccgcc aaccgtaagc
    22201 cgatatggcg gagtacaacc cgccgaaaga tatttcagcg gcaggcaggg cttgccggaa
    22261 agtccggaaa
//
LOCUS       DAWFJR010000025        22017 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWFJR010000025 DAWFJR010000000
VERSION     DAWFJR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22017
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.025"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..634)
                     /gene="mafB"
                     /locus_tag="WGR04_000885"
     CDS             complement(<1..634)
                     /gene="mafB"
                     /locus_tag="WGR04_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ0794470.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNARGSVKNRVYAVQTFDATAVGPILPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDNHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKSNIVPRAPFSDRWLKENAGAASGFFSRADEAGKLIWE"
     gene            complement(674..1636)
                     /gene="mafA"
                     /locus_tag="WGR04_000886"
     CDS             complement(674..1636)
                     /gene="mafA"
                     /locus_tag="WGR04_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0794471.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIQPYGNHMGNSAPSVEADNSHEGYGYSDEAVRRHRQGQP"
     gene            complement(1820..2539)
                     /locus_tag="WGR04_000887"
     CDS             complement(1820..2539)
                     /locus_tag="WGR04_000887"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ0794472.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVITGEDEGTLVHC"
     gene            2633..2740
                     /locus_tag="WGR04_000888"
                     /pseudo
     CDS             2633..2740
                     /locus_tag="WGR04_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2862..3716)
                     /locus_tag="WGR04_000889"
     CDS             complement(2862..3716)
                     /locus_tag="WGR04_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ0794473.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFSKENGTEVISFVRYKVGDGIEKKAVDYAA
                     EVAAAAKV"
     gene            complement(3845..4573)
                     /gene="rpsB"
                     /locus_tag="WGR04_000890"
     CDS             complement(3845..4573)
                     /gene="rpsB"
                     /locus_tag="WGR04_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ0794474.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAIRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(4750..4983)
                     /locus_tag="WGR04_000891"
     CDS             complement(4750..4983)
                     /locus_tag="WGR04_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794475.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFV
                     VTVRRRNRLMKQQEQAEFEQQRAQRQKDSGTKP"
     gene            5061..5642
                     /locus_tag="WGR04_000892"
     CDS             5061..5642
                     /locus_tag="WGR04_000892"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ0794476.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAATVKINRLLKRYIKHGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVDRLSVEAHDLPLDGFVSEAGILCF"
     gene            5703..6293
                     /locus_tag="WGR04_000893"
     CDS             5703..6293
                     /locus_tag="WGR04_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0794477.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            6830..8296
                     /locus_tag="WGR04_000894"
     CDS             6830..8296
                     /locus_tag="WGR04_000894"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ0794478.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
     gene            complement(8483..8857)
                     /locus_tag="WGR04_000895"
     CDS             complement(8483..8857)
                     /locus_tag="WGR04_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ0794479.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(9095..9394)
                     /locus_tag="WGR04_000896"
     CDS             complement(9095..9394)
                     /locus_tag="WGR04_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ0794480.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            complement(9549..10328)
                     /gene="map"
                     /locus_tag="WGR04_000897"
     CDS             complement(9549..10328)
                     /gene="map"
                     /locus_tag="WGR04_000897"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ0794481.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            complement(10368..11018)
                     /locus_tag="WGR04_000898"
     CDS             complement(10368..11018)
                     /locus_tag="WGR04_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794482.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     AAIGALAIGIVSTFNPNGDKTLQAEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            complement(11026..11634)
                     /locus_tag="WGR04_000899"
     CDS             complement(11026..11634)
                     /locus_tag="WGR04_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ0794483.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            complement(11698..12291)
                     /locus_tag="WGR04_000900"
     CDS             complement(11698..12291)
                     /locus_tag="WGR04_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ0794484.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            complement(12296..12643)
                     /locus_tag="WGR04_000901"
     CDS             complement(12296..12643)
                     /locus_tag="WGR04_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ0794485.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGVAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            12689..13564
                     /gene="rsmI"
                     /locus_tag="WGR04_000902"
     CDS             12689..13564
                     /gene="rsmI"
                     /locus_tag="WGR04_000902"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ0794486.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREAGFKVVPVVGASAVMAALSVAGVEGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            complement(13599..14004)
                     /locus_tag="WGR04_000903"
                     /pseudo
     CDS             complement(13599..14004)
                     /locus_tag="WGR04_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215060.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(14123..14269)
                     /locus_tag="WGR04_000904"
                     /pseudo
     CDS             complement(14123..14269)
                     /locus_tag="WGR04_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            14511..15665
                     /gene="obgE"
                     /locus_tag="WGR04_000905"
     CDS             14511..15665
                     /gene="obgE"
                     /locus_tag="WGR04_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ0794487.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTG
                     ETVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            15748..16797
                     /locus_tag="WGR04_000906"
     CDS             15748..16797
                     /locus_tag="WGR04_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0794488.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            17485..18906
                     /locus_tag="WGR04_000907"
     CDS             17485..18906
                     /locus_tag="WGR04_000907"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ0794489.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGKLTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPPADPMPSEAGDDYTRRFYVAMNDDFDTVKAVAVLFELAGEVNKTNDAQL
                     AGRLKALGGIIGLLQRDPIEFLQGSAASDGLSNEEIDDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            18989..19768
                     /gene="xth"
                     /locus_tag="WGR04_000908"
     CDS             18989..19768
                     /gene="xth"
                     /locus_tag="WGR04_000908"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0794490.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGYWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            19755..20099
                     /locus_tag="WGR04_000909"
     CDS             19755..20099
                     /locus_tag="WGR04_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794491.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPTWALVWLLICMPCLLIASKCLGWKGWRRVVNIFACLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            20110..20640
                     /locus_tag="WGR04_000910"
     CDS             20110..20640
                     /locus_tag="WGR04_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0794492.1"
                     /translation="MQGFELEDLTLWLIRDAGEDQMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLRTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVSEHGDVPRDWAQKQADTWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(20869..21984)
                     /gene="asd"
                     /locus_tag="WGR04_000911"
     CDS             complement(20869..21984)
                     /gene="asd"
                     /locus_tag="WGR04_000911"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ0794493.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
BASE COUNT         5043 a         5313 c         6093 g         5568 t
ORIGIN      
        1 tttcccatat cagttttcct gcttcatcag cacggctgaa aaaaccagag gcggcaccgg
       61 cattttcttt tagccagcgg tctgaaaatg gggctcgggg aacaatatta ctctttgttt
      121 ttgttgaagt tcctttgaca tagtagctgt atatatccct tgctcctccg gggggcggat
      181 aatcgctgcc ttgcggcccg tcatatccat cctccggatg gatttccgac cctgtccgat
      241 gaagttggta aacggtaaaa ccaccgtcta cgccgccgct gaaatcagaa gtgctttttg
      301 aatcatgatt atcgaacgga ctgtgtactt catgtccatg tcctgaaaaa tgggtttcat
      361 aaccgataat gccttcaaat cctgtccgtt cgtgtgtaat aggcagtatg gggccgaccg
      421 cagttgcatc aaatgtttgg acggcgtaaa cccgattttt aacactgccg cgagcattac
      481 caaacagatg gtatttacca cccggttcgt agtgttttgc ctgcatatca tcgcgcaaac
      541 gggcatccaa accgttcgca tgactaatgg ggatttgcag caacgatatt gctgctgcaa
      601 acagcatcat gaatttttga ataggcaaat tcattttgtt ttccttggta gcaagcggtt
      661 atggtagtgt gaatcaaggt tgcccttgtc tatgtcgtcg cactgcttca tcgctgtatc
      721 catacccctc atgactgtta tcagcctcta cggatggggc agagttaccc atatgattgc
      781 cgtatggttg gatatcggag aaatcgacca ttaatccttc tgtcggttta attcctttgc
      841 ttactttata cggtcccatc cacaatgcgt aattttcttt ataggcagct tcaaacgcat
      901 tggtttttgg tttgatgagc aattttttat tggttctgtc tactgcgaaa tattccagtt
      961 ttgtttgggc tttcagtgtt tcggcattgt ataggtgcat ttcggttctg ttgcgtatcg
     1021 ttccgaatac gtcgatgtta ataaacacat ccgtatcggc attggcagga gaaacaacgt
     1081 ctatgccgcg caggaaaaat acggtctgta ccaagtggga aagaaaggca gtgtcgcgcg
     1141 ggttagtcgt caaggtttca tttcgataat cccccatccc gccaatattt aagcccagac
     1201 tgcttttact tccgctaccg tctgattggg tgcgcgagag tgcaggggca ttaagtgtag
     1261 ataaagaagt ggttaaacct gtcaaaccgc ctgatgttgt ttcagcggtg gtttcgtaac
     1321 gtggataggt gtaatcggta cggacggcag ggctgtttat gtattcgcca cgaatcagtg
     1381 catcaatgga gtagcgaccc cctgtcaaac tgcctgaacc ttggtcgccc atagttgcaa
     1441 tgtacaatgc aacttttcgt ccgtgtaatg cctgtaaatc catgtcttta acggcagctc
     1501 tggcagaagc ggccacaagt tcttgttcga ccgcaaagcg tttacctccg ccatgcgatg
     1561 gaatacctgt cagtgtcccg caggcggata aaataaaaac tgaaaaaaga ataggtatca
     1621 gcagccgtgc ttgcatagat tttctccttt gatgaaaaac aaattgtatc aaaattgtaa
     1681 atatagtgga ttaaatttaa accagtacgg caaggcgagg caacgccgta ctggtttaaa
     1741 tttaatccac tataatctcc atataataaa tggctgtctg aagcccatat gcgactatat
     1801 ctgccgacac tatggtcaat cagcagtgaa ccagcgttcc ctcgtcttcg ccggtaatga
     1861 cgcgtttgag cgagccttct ttggcgatgc cgaagacgac gatattgagc ttgcgttcgc
     1921 ggcagagggc gaaagcggtc gcgtccatga ctttgaggtt tttcaacaag gcttcgtcaa
     1981 aagtaatggt ttcgtagcgt gtggcggacg ggtctttttt cgggtctgcg gtgtatacac
     2041 cgtcgacgtt ggtggctttg agcatcacgt cgcagttcat ttccgcaccg cgcaatgcgg
     2101 cggcagtgtc ggtcgtgaag aacgggttac cggtaccggc ggcaaaaatc acgactttgc
     2161 cttcttccaa atattggatg gctttaggac gggcgtaagt ttcagcgatt tgctgcatag
     2221 acagtgcgga ttgtacgcgc gctttgatgc ctaaagtttc aaatgcgtct ttgagtgcca
     2281 aggcgttcat cacggtcgcc atcataccca tgtaatcggc agtggcgcgg tccatgctgc
     2341 ctgcttgggc ggatacgccc cggaaaatat tgccgccgcc cacgacgatg ccgacctgta
     2401 cgcccatttt aacgacttcg gcaatttcgc cgacggtttg gacgatggtg tcgtgattga
     2461 tgccgaacgg atcggaaccc atcagggatt cgccggagag tttcagtaat acgcgtttgt
     2521 atttgatttg ctgtgtcatg ggataccttg ctttcttgaa tgtcgtcggt cggagttggg
     2581 ccgtctgaat ttccaacggt cggaattaca atgatatggc gtgtcgttct gttatagtgg
     2641 attaaattta aattaggaca aggcgacgaa gccgcagaca gtacaaatag tacggcaagg
     2701 cgaggcaacg ctgtactggt ttaaatttaa tccactatat tgttttgttt tcagacggcc
     2761 tttatattcg gtcgtcttaa aataggtacc gtaaacggtt ttctcaaaaa aaagcaccct
     2821 gattcgtttg gaatccaggt gctttctttt tcataagggc cttacacttt agcagcggca
     2881 gcaacttcgg ctgcgtaatc gacggctttt ttctcaatac catcgcctac tttgtagcgt
     2941 acgaagctga tcacttcagt gccgttttct ttagagaatt gggcaacagt ttgatcgggg
     3001 ttcatcacga atgcttggcc gttcagagtg atttcagcca agaatttacg gatgcggcct
     3061 tcaaccattt tagcggcgat gtcggcaggt ttgccggaag cgatggcttg ctcggtgtag
     3121 atgtggcgtt ctttttcaac ggtttcggca tctacttcgg cttcgcttac gcattgtggt
     3181 ttagcggcaa caatatgcat accgattttg cgtgctacgt cttcagagcc tttgtactca
     3241 accaatacgc cttcggtcgc caatgcgccg tggatgtagg caaccagttg gttggcagtg
     3301 tcgatcactt ggaagcgacg gacagacata ttctcgccca atttggcgat gatggctttg
     3361 cgttctgctt caaccagttc gctcagttct tcaacagaag ccggtttttt ctcggcagca
     3421 gttttcgcaa cgaagttggc aaattctacg aagcccgcgt ctttagcaac gaagtcggtt
     3481 tcgcagttta cttcgaccaa tgcgccgaca ttgccgttga tcgcgtaagc caatacgcct
     3541 tcggcagcgg tacggccggc cagtttaccg gctttcgcac cggatttgat acgcaggatt
     3601 tcttcggctt tgtcgaagtt gccttcggct tcaaccaagg cttttttgca ttccatcatg
     3661 cccaggccgg tagcggcgcg caggtcggca accatttttg cagtaatttc tgccattttg
     3721 aatctcctag atttttggga aatacggaaa ggattttccg cattcgggaa tcgtgtgccg
     3781 cgaacggacg ttcagacggc atatttgaga aaaggggcat aacgcccctc ttcggtttgc
     3841 cggattactc ggcagcggct tcttgggcag cggctacggt ttcttgcagc gcttggtttt
     3901 tgccttccaa aactgcgtca gcgatgccgc ggcagtacag gcggatggct ttggcggagt
     3961 cgtcgttgcc ggggatgacg tatttcacgc cgtcagggct gttgttggta tcgactacgg
     4021 cgataacggg gatgcccagt ttttcagcct cgaccagagt acctttttgg tagccggtat
     4081 cgataacgaa aatcgcgtcg ggcaggcctt tcatgttttt gataccgccc aaagaacgtt
     4141 ccagtttttc aacatcgcgt tgcatttcca gaatttcttt tttgctgaaa ccgctttcgg
     4201 cagcattttc caaggctgcg gttttttctt ccaggcgttt gatggattgc ttaacggttt
     4261 tgtagttggt cagcataccg cccaaccagc ggtaatcgac gaaaggcata ccggcgcggg
     4321 tcgcttcttc gcggatgatg tcgcgtgcct ggcgtttggt acctacgaac aatactgtac
     4381 ctttgttggc aaccagacgg cgtatggctt cttgcgcgtc ttggaacatc ggcagggttt
     4441 tttccaggtt gacgatatgg attttgttgc gcgcaccgaa aatgtattgt gccattttcg
     4501 ggttccagaa acgggtttgg tggccgaagt ggacaccggc ttcaatcatc tgacgcatag
     4561 taatttgaga cattttgatt tccttgaaag ggttaagggc acacattcaa tcgcatagaa
     4621 cttgataggc gacgcctatt gtcaagcacc cttcggcgaa agtgggcata agttttaaaa
     4681 aataaaagag tgtttccgaa atggaaaact gacggattat agcggataag aggccgtctg
     4741 aaaagggatt cagggttttg tgccgctgtc tttttgccgt tgtgcacgct gctgttcgaa
     4801 ttctgcctgt tcctgttgct tcatcaagcg gttgcggcgg cggacggtta cgacgaagac
     4861 cgtgaacacg gtgggcagca ccgcccaaaa aaccaaataa atcaaggcgc gcgcaatact
     4921 cggctgcgcg gcggaataca taacggcaac aaaaagatag ccgatggcga cgatgtgcca
     4981 cattgtgtgc gtcctgtttg aatgttaaca aaatcgttat aatcgcaaca ttctaccgca
     5041 atcctgaaag gccgtctgaa atgaggaatg aggaaaaacg cgccctgcgc cgcgaattgc
     5101 gcgggcggcg ttcgcaaatg gggcgggacg tgcgggcggc ggcaacggta aaaattaacc
     5161 gcctgctcaa acgttatatc aagcacggtc ggaaaatcgg cgtgtattgg ccgatgggca
     5221 aggaattgcg tttggacggc tttgtccgcg cggcgcaaaa acgcggcgca aaactttatc
     5281 tgccttatat cgaaccgcgt tcgcggcgga tgtggtttac gccgtatcct gaaagcggaa
     5341 tggaacggga gcgcatacgg ggcagggcga ggttgaacgt gccgcagttt gcagggcgca
     5401 aaatccgcgt gcacggtttg tcggtattgc tcgttccgct tgtcggcata gaccgcgaag
     5461 gctaccgttt ggggcaggca ggcggctatt acgatgcgac gcttgcagcg atgaaatacc
     5521 gtttgcaggc aaaaaccgtg ggcgtgggct ttgcctgcca gttggtggac agactgtcgg
     5581 tcgaggcgca cgacctgccg ctggacggtt ttgtgtcgga ggcggggata ttgtgttttt
     5641 agccgttcag acggcatttt gaacggaaat gccgtctgaa ggtggaatat tgggagaaaa
     5701 ggatgtcgga aaagataata ctgccggtct tgtcgttggg tggcgtacgg ctcgaaccgt
     5761 tggatgtgca tcatgaaacg ggtttgcgcg aggcggtttg cgatggggaa gtttggaagc
     5821 tgggagtaac gtccgcgccc catccggata gggtggcaga ctatatcggg acagcattgg
     5881 caacgcgttt ggcgtttgcg gttgtcgatg aagaggcggg cagggtggtc gggacaacgg
     5941 cgtattatca ttttgaaccg cagattccgc gtttggatat tggattcaca tggtatgcgg
     6001 catcggcacg gcggacacgg ataaacacct gttgcaaaat catgctgttg gattacgcat
     6061 ttgatgtttt ggcttgccgt tgcgtgggat ggcgcacgga tattctgaac cttgcctcgc
     6121 aacgtgccat cgaacggctg ggtgcggaaa aagacggcgt gctgcgtatg catatgctcc
     6181 gtaaagacgg cagtgtgcgc gatacggtcg tgtacagtat gctgagggaa gattggtgca
     6241 aaaacaggga aatcctgacc ggcaggttgg cagggtatgg cgtgcaggtg tgaggcagta
     6301 ttttcaaatt tatagtggat taacaaaaac cagtacagcg ttgcctcgcc ttagctcaaa
     6361 gagaacgatt ctctaaggtg ctgaagcacc aagtgaatcg gttctgtact atttgtactg
     6421 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctataaaaaa cttaacgcaa
     6481 tatgctgaca atcttttaaa aattaatata taaacaattt tgatttttta tctattaaaa
     6541 atttgatata tatagataaa taactatttt tatttttcat ttcgcagttt cccgacttgg
     6601 cgggcgacag aaaaactttg catttcccag taaaatccac tacattcacg ataacatatt
     6661 acaagcccct gaagcggtgt cggaagccat gcttgcaacg cattccaacc ccgaaacagc
     6721 atactcggac acacaccatt gccggttttc cctgcttttg cgattcaggc gtttgtagta
     6781 cggcaaataa ccataagggc agaagatgcc tgttttttag gagtaaacca tggctgaagc
     6841 gacagacgtt gtcttggtgg gcggcggcat tatgagcgcg actttgggcg ttttgctcaa
     6901 agaactcgaa ccgtcttggg aaatcaccct gattgaacgc ttggaagatg tggcgttgga
     6961 atcgtcaaac gcgtggaaca acgccggcac ggggcattcc gcgctgtgcg aattgaacta
     7021 tgcgccgttg ggtgcaaatg ggattatcga tccggcgcgc gccctcaata ttgccgaaca
     7081 gtttcatgtc agccgccagt tttgggcgac gctggtcgcg gaaggcaagt tggaagacaa
     7141 ttccttcatc aatgccgtgc cgcatatgtc tttggtgatg aatgaagacc attgttctta
     7201 tcttcaaaaa cgttatgacg cgtttaaaac ccaaaaactt tttgaaaata tggaattttc
     7261 caccgatcgg aacaaaattt ccgattgggc tccgctgatg atgcgcggcc gggacgaaaa
     7321 ccaacccgtc gccgccaact actccgccga aggtacggat gtcgatttcg gacggctgac
     7381 gcgccaaatg gtgaaatatt tgcagggcaa gggcgtaaaa accgagttca accgccacgt
     7441 cgaagacatc aaacgcgaat ccgacggcgc gtgggtgctc aaaaccgccg atacccgcaa
     7501 ccccgacggg cagctcaccc tccgtacccg cttcctcttc ctcggcgcgg gcggcggcgc
     7561 gctgaccctg ctgcaaaaat ccggcatccc cgaaggcaaa ggctacggcg gcttccccgt
     7621 gtccggcctg ttcttccgca acagcaaccc cgaaaccgcc gaacaacaca acgccaaagt
     7681 gtacgggcag gcttccgtcg gcgcgccgcc gatgtccgtc ccgcacctcg acacacgcaa
     7741 cgtggacggc aaacgccacc ttatgttcgg cccttacgca ggcttccgtt ccaacttcct
     7801 caagcaaggc tcgcttatgg atttgccgct gtccatccat atggacaacc tctatcctat
     7861 gctgtgcgcc ggctgggcga atatgccgct gaccaaatac ctgctgggcg aattgcgtaa
     7921 aaccaaagaa gaacgcttcg cctccctgct ggaatactac cccgaggcaa accccgacga
     7981 ctgggaactc atcaccgcag ggcaacgcgt ccaaatcatt aaaaaagact ccgaaaaagg
     8041 cggcgtgctc cagtttggta cggagattgt cgcccacgcc gacggctcac tcgccgcatt
     8101 gctgggcgcg tcgccgggcg catcgaccgc tgtgccgctg atgatccggc tgatgcacca
     8161 atgcttcccc gagcgcgccc cgtcttggga aggtcgtctg aaagagctgg taccgggtta
     8221 cggcatcaag ttgaacgaaa accctgaaag ggcggatgaa attatcgcct ataccgcgaa
     8281 agtgttggat atttaagccg aatctttcaa gaaaagccgt acccccatat ggtgttgatg
     8341 ccgtccctgc cggttatgcc cttcatccgg cagggcaggt tttggtacac ggggcaggta
     8401 atcgtttttt cccttttgtg aataatggaa cagtcaaaaa acagcgtttt catttctgaa
     8461 aacgctgttt tttgttgcct gattaacgtg gggaacagtc tttgaagacg atttggttgt
     8521 caggtgcggt aatcataatg ggctgtttgc ggtaggattt accatccatc acgccggtac
     8581 ccaaaacata accgccttct ttgccgtaga atgtttccac attgtcggat ttgtccaaat
     8641 tgacaggcat ttgcacgcgt ttgccgttga tgacggcgga agcgtatgtg gtcaggccct
     8701 gtttgttaaa gccgtaggtt actttgactt ttttaccttg ctggcagacg tagctgacgg
     8761 tttttttggc aacggtgggg ttgttcgtgc cggcagcagc agccatactg ctgagcgcga
     8821 ttgcggaaga caggattgcg gtggtcagaa gtttcatttt tattcctttg gatggatgtg
     8881 gagtttgccc gtttcgagct gtttgacatt ctaatgtcgg cgtatcctga aataaacagg
     8941 aaattggata aagtttgtaa aaaggcgggt tcgggcggtt cggcggtttg aggcaggggc
     9001 ggcaggcggg atacggcggc ctgtccggtg tttcagacgg cattgtttgc gtttttcgga
     9061 aatgaaaaac cttcgggcat ccggaggttg cggtttaact ttgttttttt ctttcggact
     9121 tggtctgttt ggacaattcg gcatacaggt cgggatggtg ttttttcaac agggcggcga
     9181 tttccatatc ttttttgttg actttcgcca aatcgatgca ttcgatatgg acgaggcgga
     9241 gcagttcgcg tacgggtttg ggcattttgc ggccggtttc gtagcgcgag cctccagact
     9301 gggtaacgcc gaccttgccc cagaagtcta tctggttcag cccggtcttt ttacggatgg
     9361 ctttaacatc ttctatattt tcaaatattt tcataaaact tcctttaagt ttgtctggtt
     9421 gcggggcaaa ttccgatttg ctttgttttc gtaatgtgcc gggtcaagcc tgtttttata
     9481 ttatatatca tatctgccgt ttctttcaga cggcattgtt tgtttttatg gggcggatac
     9541 gtctggtttc agggtttgcc ggaggcgggg ctgacggtga ggatttcgta gccggtttcg
     9601 gtcaccaaga cttcgtgttc ccattgggcg gagagggagc ggtctttggt aaccaccgtc
     9661 cagccgtcgt tgaggatacg caggtggcgt ttgccttggt tgatcatcgg ttcgacggta
     9721 aaaatcatac ccggttttag aacggggccc tgtccttttt ttccgtagtg caacacttgc
     9781 ggggcttcgt ggaaaccgcg cccgatgccg tgtccgcaga attcctgtac gacggaatag
     9841 ccggcgtttt cggcaacctg ttggcaggcg taacctacgt cgcccagtgt cgcgccgggt
     9901 ttgacggctt ctatgcccgc catcatggag gcgtgggtta cgtcgatcag gcgttgggcg
     9961 atgggggaga ctttgccgac ggtaaacata cggctggagt cgccgtggaa gccgtctttt
    10021 ttgatggtga ggtcgatgtt gataatgtcg ccttctttga gcggcttgtc gtcgggaatg
    10081 ccgtggcaga tgacgtggtt gacggaggtg cagcaggatt tggggtaggg cgggttgccg
    10141 tagtgcaggg gggcgggata gccgccttgg acgttgacgt ggtagtcgta aacgagtttg
    10201 tcgatttcgt cggtggttac gccgggtttg acgaattgtc cgatgtagtc gagggcttcg
    10261 gcgacgagtt tgcccagctc gcgcattttt tcgatttctt cgggggtttt gatgatgatg
    10321 ccgttcataa tgcttccttg gattgcgggt gtgcctgccg tgccgggtca gaagaggttg
    10381 tcgagaattt ctttatgggg tttgggcggc gtgtttttcg gggtgtccgg tttggtatgg
    10441 ttttctttgg gcgtttcttt ttctttgggt gtttctttgg gctgcgcctg tttttcgggt
    10501 gtttcttttc ggggttcggg acgggggacg tttgtgggca gtacgggggt ttctttgagt
    10561 gtgttttcag cttgtgttcc ggcgtcgtcc ggctgcctgt cggtttgagc tgtgtcggca
    10621 ggttgcggtt tgacccggtt tttcttgggt gcggcagggg acggcagtcc gctgccgctt
    10681 tcgtctgtgc cgtccggctg tgcgggttcg gatgaggcgg cgtggtggtg ttcgggttgg
    10741 gcggcatctt gtccgactac gccgtttggc agccagaatt cggtttcgcg ggggctgtcg
    10801 gtgtgttgcg gttcggcttg aagggttttg tcgccgttcg ggttgaatgt gctgacgatg
    10861 cctattgcca atgcgccgat ggcggcgata cagatgagca atacggcgcg tatcaggagt
    10921 ttgggggtca gcctgaaggg tttgttcggt ttttttgcca ttttgattgt gcttttgggg
    10981 tgtcgggcaa tgccgtctga aggcggttca gacggcattg ccgagtcagc gttggacgta
    11041 gttttggtag agggtgatga ctttttgtac gccgacggtg gtgctgactt tttgggtaat
    11101 ctgcgcctgt tcttcggggg tgaggatgcc cataacgtag gttacgttgc cgtaggtaac
    11161 gattttgacg cgcgcctgtg tggcggggct gatgcccaac agcgtggcgc ggactttgga
    11221 tgtgttccaa gtgtcgccgg cgatgtcgcc ggcagtgcgc ggcagggagg cgacggtaat
    11281 atagttgtac acgccttcgg cggcctgttc ggaacgtgca atctgaccga cgaactgttt
    11341 ttcgccttcg gtggcgactt gtccgagcag cagcaggtgg cggttgtagc cgacgacgga
    11401 gatttggggc gtgtagcctt tggtttggtt gttttggcgc agataggaac gggcggtggt
    11461 ttcgatacgc aacgccataa cgttgtcgtc ggtttgcgcg ccggtggttc ggcggtcgac
    11521 ggcggatttc gcgccgacgg cggcgcttcc gattactgcg ctgacgcagc cgctaagggc
    11581 aaggctgaaa atggcggcaa tcagggtgcg gacggtgtgc ggtttgggtt tcatcgggtg
    11641 cttcctttct tgggcgtttc agacggcatt gctttgcgcc atgccgtctg aaaagggtta
    11701 cattccttcc agcagtacgg agtcgataca gtcgcacatg gcgtgtatca gcaggatgtg
    11761 gttttcttga atacgggcgg tgcgcggatg gggaacgttg agcaaaacgt cggtgtcttt
    11821 gagtatggcg gcgattttgc cgccgtcgcg gccggtcaag gcgatgacgt gcatatcgcg
    11881 ttcgtgtgcg gctttgacgg cttcgatgac gttggcggaa ttgccggagg tggaaatgcc
    11941 gaccaataca tcgcctgcac gtccgagcgc gcgcacctgt ttgctgaata cgtggtcgaa
    12001 accgtagtcg ttgccgatgg ctgtcagcgc ggaagtgtct gttgtcagcg cgacagcggc
    12061 gagttccatg cgttcttttt caaaacggcc ggtcatttcg gcggcgaagt gttgcgcgtc
    12121 ggcagccgaa ccgccgttgc cgcaggccag gattttgccg tcattcatca ggcattgcag
    12181 catcagctcg gcagcctgta cggtcggctc gaccaatact tttccggctt cctgcttggc
    12241 acggatgctt tcggcaaaat gggcggcaac gcgttcttgt aatgtcgtca tatcgtcaac
    12301 ctgtaatatt ctgtatccac tcgggcgggc ggctgccttc gataagtacc gcatcgaggc
    12361 ggcacggtac gtttgtcaac ctgttctgtt gcagataata ctctacactt cgttgcagtt
    12421 tcaataattt ggatggggaa atgctgtatg cgacaccgcc gaattgccga tttttgcggt
    12481 attttacttc aacaaacaga atcatgccgc cgtttttgac aatcaggtcg atttcgccgt
    12541 aggcgcagtg ccagttgcgg gcaagcagcg tgcagccttg ggattggagg aaggcaagcg
    12601 cggcatcttc ccctgcctcg ccctgtttgt ggtttaggcg cataatcggt ttaaccttta
    12661 taatgtttgt ttttcagacg gcattcttat gtttcagaaa catttgcaga aagcctccga
    12721 cagcgtcgtc ggagggacat tatacgtggt tgccacgccc atcggcaatt tggcggacat
    12781 taccctgcgc gctttggcgg tattgcaaaa ggcggacatc atctgtgccg aagacacgcg
    12841 cgttaccgca cagcttttga gcgcgtacgg cattcagggc aaactcgtca gtgtgcgcga
    12901 acacaacgaa cggcagatgg cggacaagat tgtcggctat ctttcagacg gcatggttgt
    12961 ggcacaggtt tccgatgcgg gtacgccggc cgtgtgcgac ccgggcgcga aactcgcccg
    13021 ccgcgtgcgt gaggccgggt ttaaagtcgt tcccgtcgtg ggcgcaagcg cggtgatggc
    13081 ggctttgagc gtggccggtg tggaaggatc cgatttttat ttcaacggtt ttgtaccgcc
    13141 gaaatcggga gaacgcagga aactgtttgc caaatgggtg cgggcggcgt ttcctatcgt
    13201 catgtttgaa acgccgcacc gcatcggtgc gacgcttgcc gatatggcgg aactgttccc
    13261 cgaacgccga ttaatgctgg cgcgcgaaat tacgaaaacg tttgaaacgt tcttaagcgg
    13321 cacggttggg gaaattcaga cggcattgtc tgccgacggc aaccaatcgc gcggcgagat
    13381 ggtgttggtg ctttatccgg cgcaggatga aaaacacgaa ggcttgtccg agtccgcgca
    13441 aaacatcatg aaaatcctca cagccgagct gccgaccaaa caggcggcgg agcttgctgc
    13501 caaaatcacg ggcgagggaa agaaagcttt gtacgatctg gctctgtctt ggaaaaacaa
    13561 atagtgccgg acgagccggc accatagtgt gaacggaatc agcggacacg acccgtcatg
    13621 cggcacagaa catctctacc ctgaacacgg tgggcgacga ccatggcgac gtgtccggcg
    13681 acggcggcaa acagcagcaa gcccaaattg ccgtggaacg tgttgcccaa gtttgccatc
    13741 cactcgattt tttccggcga acccttgcat cacttcaacg ccgaacactt tcaacgggcc
    13801 gcggccgctg ccgtattggc ggatcatgcc gataacggga acagcaagca tgagcagata
    13861 cagaatgccg tggcctgccg ccgcagcctt gcagtcgctt tgcggacgct tggcgcggtt
    13921 ggcaaccgcc cacacgatgc gcaatgtaat caccgtcagc gtaaggaaac cgaaagattt
    13981 gtgcaggccg aacaggctgc ccacccattc cgcctcttcg tatagtggat taacaaaaac
    14041 cagtacggcg ttgcctcgcc ttgccgtact atttgtactg tctgcggctt cgtcgccttg
    14101 tcctgatttt tgttaatcca ctataaatcg tccacaggac aatggtggtc agaatgccga
    14161 tgaagccggc aacggtcagc cagtgcagcg cgcgtgtcaa cgggtcgtaa acggcaggtt
    14221 ggtttgtgtt cataaaaaac ttattatatg taacggtttg aacggacatt ccgtcgggtc
    14281 ggaatgtcaa aaaggcgcga ttgtaccaaa gaagttggac ataattttgt ttgcaggctg
    14341 aagatttgct taaaaattca ttaagatggg cggaacaaat agtttgggta cggcgtgttg
    14401 aaataccgct tatcccttat aatagcgtcc gaaattctgt tcggacggca tcaaaacaca
    14461 cggtaatccg tctgaacccc ccatttgaaa ccaacaaaca agagcattga atgaaattca
    14521 tcgacgaagc aaaaatcgaa gtcgccgcag gcaaaggcgg taatggcgca accagtttcc
    14581 gccgcgaaaa attcgtaccg cgcggcggcc cggacggcgg cgacggcggc aaaggcggca
    14641 gcgtctgggc agaagccgac gaaaacacca acaccctcgt cgaataccgc ttcgttaaac
    14701 gctaccaagc caaaaacggc gaaaaaggcc acggttccga ccgttacggc gcaggtgcgg
    14761 acgacatcgt cctcaaaatg cccgtcggca cccttatccg cgacctcgac accggcgaaa
    14821 ccgttgccga cctcacttat cacggacagc gcgtctgcct cgccaaaggc ggcaaaggcg
    14881 gcttgggcaa catccacttc aaatcgtccg tcaaccgcgc cccgaaacaa tccacccccg
    14941 gcgaagaagg cgaagcccgt tccctgcaac tcgaacttaa agtcctcgcc gatgtcggct
    15001 tattgggtat gcccaacgcc ggcaaatcca ccctgattac cgccgtatcc gccgcacgcc
    15061 ccaaaatcgc caactacccc ttcaccaccc tgcatccaaa cttaggcgtg gtacgcatcg
    15121 acgaaaacca cagcttcgtg atggccgaca tccccggcct gattgaaggc gcggcagaag
    15181 gcgcaggcct cggccatcgt ttcctcaaac acttatcacg taccggcctg ctgctgcacg
    15241 tcgtcgattt ggcgcccttc gacgaaaccg tcaaccccgc cgaagaagca ctcgccatca
    15301 tcaacgaatt acgcaaatac gacgaagaac tctacggcaa accgcgctgg ctggtactga
    15361 acaaactcga tatgcttgac gaagaagaag cccaaacgcg cactgccgcc ttcctcgaag
    15421 ccgtcggctg ggactacccc aaacccgacg accgtttcca atttgacatg gaaaccccgc
    15481 gcctcttcca aatcagtgcg ctgacccacc aaggcacgca ggaattggta caccaaatca
    15541 accaatacct gaccgagaaa aaacgtatcg aagctgaaaa agccgaagcg gagaaagcag
    15601 cggcaaatgt tgaaattatc gaacagcagc ctaaaacgga tacgggcgtg tttaagccgg
    15661 agtgaaaaaa gaaagggttt cagacgatat ttgttctaca aaaggttgtt tgaaacaatt
    15721 ttaaagcaag attgaaatgg attaaaaatg tcatctaacc aatatatcca gtcgcttgag
    15781 ttgacaaact ttaccgttct tccaaacgat acttttgaat tttctgaaaa tctcaatgtt
    15841 attgttgccg aaaacggttg tggcaaaaca catttgttaa aaattttata cagcctgcta
    15901 gaagtaacat ctaacaccaa aaacagatta ctgaaaacag aattgcaaaa aagttttgcc
    15961 gataaattac tgaatgtttt ccgtcccgat tccttgggca ggttgagcaa acgtttgcag
    16021 gggcgcgggc gtacggaaat cgttttaaaa ctgcaaaacg gcactacgca tagtagtctg
    16081 aatttttcca gtaactcatc atcgcaagtc aatgttcaaa gcatcggatt gaaagaaaat
    16141 gaatatactc cgacacctat cttcttaccc agccgcgaat taattacact ttgcccttgg
    16201 ttcacttctt tgtatcaaaa ccaaagcatc cctttcgaag aaacttggtt tgatacctgt
    16261 atgcaattaa atcacccgct tgccaaaggc cccagagaaa caaaaattcg agaattgctc
    16321 gaacctattg agaacgcgat gggcggaaaa gtatctgaag aacaaggaag gttttatcta
    16381 tcgctgacaa atacgggcgg taagattgaa gcacctttgg tagcagaggg tttgcggaaa
    16441 tttgtcatga ttgcccggtt aatcgcaaca ggggcattgc tggataaagg ctatctattt
    16501 tgggatgaac cggaagctaa tctgaatcct aaattgatca aagtggctgc ccgtattatt
    16561 tggtctttat ctcaacaggg tattcaggtt tttattgcaa cacactcttt attcttattg
    16621 agagaattgg aacttttgaa gcttgaaaat aacacggatt tacctgtacg ttttttctcg
    16681 ttgattgctt ccgatgatgg aacaaaagtg gagcaaggga acagtattaa tgatttgaac
    16741 acattgatat tgttagatga aaacattatg caggcagaca ggtatctgaa tatttgaggt
    16801 taagctatga ctacaatcac agaaaagaga ttaacctttg cttttcctga agattattac
    16861 gttaccaagt atgacgaatg ggaacattat aaaatttttc agaacagttg taatttacgt
    16921 aataaaattg atacaaatga aaagggaaag aacgggataa atcaaagtgt agatgacgat
    16981 aatggcagct caggtgtaga tataatagcc tttcatgaat cgacactttg gctaatcgaa
    17041 attaaagatt attaccgact tgggcttgag ccaaatgccc aatcaataga tgaaaaactt
    17101 tctgacctac catatttaat cgcaagaaaa attcgagatt cattggcagg attggtttct
    17161 gctaaattta aagcagcaaa acaggaagaa aaagattttt ctcgcttggc tttagattgt
    17221 aacgaaataa aaatagtctt tcatattgaa atgcctagta taagaagtaa actttatccc
    17281 tcgtcatcag atttagcaaa cttattaaaa gataagttta aattatcaga atttacaaaa
    17341 aattttgcaa actgttatgc agaaccaatt ttcacaaata tcagccatat taataatccg
    17401 caactgcgta atgttccttg gtccgtatct acaggaacag aacagaaatt atcatccgag
    17461 caacaaagat taatccataa tcccatgacc accatctaca acacccttac ccgccaaaaa
    17521 gaaccctttg cccccatcga ccctgaaaac gtgcgtatgt acgtctgcgg tatgaccgtt
    17581 tacgattact gccatttggg acacgcccgc gtgatggtgg tgttcgacat gattgcccgt
    17641 tggttgcgcg agtgcggcta tccgctcact tatgtacgca acatcaccga catcgacgat
    17701 aaaatcattg cccgcgcggc tgaaaacggc gagaccatcg gcaaactgac tgcgcgtttc
    17761 atccaagcca tgcacgaaga tgccgatgct ttgggcgtgt tgcgtccgga tattgagccg
    17821 aaggcaacgg aaaacattcc gcaaatgatt gccatgattg aaaccctgat tcaaaacggc
    17881 aaggcatatc ctgccgcaaa tggcgacgtt tactacgccg tgcgcgagtt tgccgcttac
    17941 ggacaactgt cgggcaaatc gttggacgac ctgcgtgcgg gcgaacgcgt ggaagtggac
    18001 ggtttcaaac gcgatccgct tgattttgtg ttgtggaaag cggccaaagc aggcgaaccg
    18061 gcgtgggaaa gcccgtgggg caacggccgt ccgggttggc acattgaatg ctctgccatg
    18121 agtgaaaacc tgttcggcga taccttcgac atccacggcg gcggcgcgga tttgcagttt
    18181 ccgcaccacg aaaacgaaat tgcccaaagc gtcggcgcga cggggcatac ctgcggtcat
    18241 catcacgcgc aaacccacca cgggcaaagc atcgccagcc acgtcaaata ctggctgcac
    18301 aacggcttta tccgcgtgga cggcgaaaag atgtccaaat cgctgggcaa cttcttcacc
    18361 atccgcgaag tgttgaaaca atacgatccg gaagtcgtgc gcttcttcat cctgcgcgcc
    18421 cactaccgca gcccgttgaa ctattccgac gcgcatttgg atgatgcaaa aggcgcgttg
    18481 acgcgtttgt atacgacttt gaaaaatacg ccgcccgccg atccgatgcc gtctgaagcc
    18541 ggcgatgact acacgcgccg cttttatgtt gcgatgaatg atgattttga tacggtgaaa
    18601 gcggtggcgg tgttgttcga acttgccggc gaagtgaaca aaaccaatga cgcgcaactg
    18661 gcaggccgtc tgaaagcctt gggcggcatc atcggcttgc tgcaacgcga tccgattgag
    18721 ttcctgcaag gcagcgcggc ttcagacggc ctctccaacg aggaaatcga cgatttgatc
    18781 gcccgccgca aacaggcacg cgcggataaa aactgggcgg aatccgaccg catccgcgac
    18841 cttctgaacg aacacaaaat cattttggaa gacaacgccg gcggcacgac ttggcggcgc
    18901 ggttaaaatc catcgaaaag ggtaaatgtg ccgcattttg cccttttttg ttcagacggc
    18961 atttttattg tgagaactta tttttattat gcttaaaatc atttccgcca acgtcaacgg
    19021 catccgctcc gcctacaaaa aaggttttta cgaatacatc gccgcatcgg gcgcggacat
    19081 tgtctgcgtg caggaactca aagcgcagga ggctgatttg tctgccgata tgaaaaatcc
    19141 gcatgggatg cacggctatt ggcattgcgc cgagaagcgc ggttacagcg gcgtggcggt
    19201 gtacagcaaa cgcaaacccg acaatgtgca aatcggtatg ggcattgaag aattcgacag
    19261 ggaagggcgg tttgtgcgtt gcgatttcga caggttgagc gttatttcgc tttacttgcc
    19321 cagcggcagc agcgcggaag aacgccagca ggtgaaatac cgtttccttg atgcgtttta
    19381 ccctatgctc gaagcgatga aaaacgaagg acgcgacatt gtcgtttgcg gcgactggaa
    19441 catcgcccac caaaacatcg acctgaaaaa ctggaaaggc aatcagaaaa attcgggctt
    19501 cctaccggaa gaacgcgagt ggataggcaa ggttatccac aagctcggtt ggacggatat
    19561 gtggcgcacg ctttatcccg atgtgccggg ctacacgtgg tggagcaacc gcgggcaggc
    19621 gtatgcgaaa gatgtcgggt ggcgcatcga ttatcagatg gttacgcccg aacttgccgc
    19681 caaagccgtg tccgcacacg tttataaaga tgaaaaattt tccgaccatg cgccgctggt
    19741 catggagtat gactatgctg ccgaataaag ttttgggtaa atatgattgg aatgtggacg
    19801 gaaaaacagg aataggcgca gcatgggttg tcgctgcgtt tatcctgccc atattggtgt
    19861 gggcgatatt tatgttgtcg cggatgcaag gctggcttgc gccgaccaag gcaaacccga
    19921 catgggcgtt ggtgtggctg ctgatctgca tgccctgcct gctgattgcg tccaaatgtt
    19981 tgggttggaa aggctggcgg cgggtggtga atatttttgc ctgcctgacc gtctgcgcca
    20041 tattgagcgt acccgcttcg ctgctcatcg cctttaccct ccgggacctg ctgaaatagg
    20101 cggggcggta tgcagggttt tgaactcgaa gatttgacgc tttggctgat acgcgatgcc
    20161 ggagaggatc agatgtggat agaccgttgg gcgatcagtt atcccgtcgt gcagatgtcc
    20221 gaagcgtcgg ccggtcaaag cataggggaa tggcaggcag ggcttcggac ggcatttgaa
    20281 cgcatacgtg gcaaatacgt cgccgttgtc gcacacggtg cgggcgcggc cgcatttttg
    20341 gcgtggctgt atcaggcaga catcctgaca cggaagaaaa ttgccaacat catccttgta
    20401 ccgcagcgtc ccgatatttt tcccgacgat gcggaacaca ctttccaacg cgtccgctgt
    20461 ccctgccgtg ccgcattggt ggtgtccgaa cacggggatg tgccgcgaga ttgggcgcaa
    20521 aaacaggcgg atacgtggaa cgcgcgcctg ttggtttccc cgcattcggg cagtttgaac
    20581 ggtatgctcg gcggctggca gtggggtatg aagctgatgc aggaaatgtt gctggcgtga
    20641 acgcttgcct tatcggaatt tcggctgtag caggaagtcc gcatatcggg ctgttttcat
    20701 tgacgtgcag gcgcatcagg ttcggataaa gtcgggctgc cgcataccgc cggaaatgga
    20761 atcctttatt ttttacgtcg gcagtgccgg cagggcgtta tccggcaggg ttgcaaggaa
    20821 aatgccgtct gaaacaggct tcagacggca tttccattca aacagggctt acaggctgcc
    20881 caacacgata cgcaatacgc ggcgcagcgg ctcggccgcg ccccacaaga gctggtcgcc
    20941 gacggtgaac gcgctgatgt attcgccgcc catgcccagt ttgcggatgc gtccgacagg
    21001 gacggacagc gtgccggtaa ctttggcagg agtcagctcg tggatgctgg cttctttttc
    21061 attggggatg actttcaccc agtcattcgc gcctgccaaa atcgtttcga tttcggaaac
    21121 aggcaggtct tttttcaact tcagagtgat ggcttggctg tggcagcgca tcgcgccgac
    21181 gcggacgcac aggccgtcaa tcacggttgg attgtcgctg cggccgagga ttttgttggt
    21241 ttccacgccg cctttccatt cttctttgga ctggccgttg cccaaatcca cgtcaatcca
    21301 cggaatcagg ctgccggcga gcggtacgcc gaagttggct ttcggatagt cttcgctgcg
    21361 caggaaatcg gatactttgc ggtcgatgtc gagaatcgag ccggcaggat cggcaagttc
    21421 gtccgccact tgggcgtgaa ccgcgcccat accgctgatg agttcgcgca tgtttttcgc
    21481 acctgcgccg gaagcggctt ggtaggtcat gctggttgcc cattcgacca aatcgttttg
    21541 gaacaggccg cccaaagcca tcagcatcag ggagacggtg cagttgccgc cgatgtagtt
    21601 tttcacgccg tttttgaggc cgttgtcgat aacgttgcgg ttgacagggt cgaggacgat
    21661 aatcgcgtcg tctttcatgc gcagagaaga tgccgcgtca acccagtagc cgttccagcc
    21721 gctgtcgcgc agggcttgga agacggattt ggtgtaatcg ccgccttggc aggtaacgat
    21781 gatgtccatt ttggcgagtt cggcaacatt gttggcatct aataatgttt tagccgcctg
    21841 accgaaatca ggggctgcgc cgccgacgtt ggaagtggta aagaaaaacg cttcaggaat
    21901 gtgggcgaag tcgttttctt ctttcatacg ctgcatcaaa accgaaccga ccataccgcg
    21961 ccagccgacg aaacctactt tcattgtgtt gctccttgag gaaaaatcgt taatata
//
LOCUS       DAWFJR010000026        21529 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWFJR010000026 DAWFJR010000000
VERSION     DAWFJR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21529
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.026"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..380)
                     /locus_tag="WGR04_000912"
     CDS             complement(<1..380)
                     /locus_tag="WGR04_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794494.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMY"
     gene            complement(438..803)
                     /locus_tag="WGR04_000913"
     CDS             complement(438..803)
                     /locus_tag="WGR04_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794495.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(785..1330)
                     /locus_tag="WGR04_000914"
     CDS             complement(785..1330)
                     /locus_tag="WGR04_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794496.1"
                     /translation="MKKQITAAVMMLSMIAPAMANSMDNQIFENQVFHTRADAPMQLA
                     ELSQKEMKETERAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
     gene            complement(1493..1765)
                     /locus_tag="WGR04_000915"
     CDS             complement(1493..1765)
                     /locus_tag="WGR04_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794497.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINIIIS
                     YVLIKNILVSVSDEGGIKYVAIYLSNLFFWTVLMYVLVKRLSKKPS"
     gene            complement(1834..2268)
                     /locus_tag="WGR04_000916"
     CDS             complement(1834..2268)
                     /locus_tag="WGR04_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794498.1"
                     /translation="MKKQITSAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGEWIPNAVGGIMGGVGGHFSYMASAIASGSYNRNEHWTAIGGGALIG
                     AMNPINGGRALINGMRGVAVGTVAGGINGYAGSTGKNTDIRR"
     gene            complement(2311..3180)
                     /locus_tag="WGR04_000917"
     CDS             complement(2311..3180)
                     /locus_tag="WGR04_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ0794499.1"
                     /translation="MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            3344..4267
                     /locus_tag="WGR04_000918"
     CDS             3344..4267
                     /locus_tag="WGR04_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0794500.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHSLFEPGKVMFYYRETSRESEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(4345..4986)
                     /locus_tag="WGR04_000919"
     CDS             complement(4345..4986)
                     /locus_tag="WGR04_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0794501.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSGGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARTPDLLLLIGAAVCHYTDRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(5041..5832)
                     /locus_tag="WGR04_000920"
     CDS             complement(5041..5832)
                     /locus_tag="WGR04_000920"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ0794502.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            5998..6073
                     /locus_tag="WGR04_000921"
     tRNA            5998..6073
                     /locus_tag="WGR04_000921"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6031..6033,aa:Thr,seq:tgt)
     gene            6108..6183
                     /locus_tag="WGR04_000922"
     tRNA            6108..6183
                     /locus_tag="WGR04_000922"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6141..6143,aa:Thr,seq:tgt)
     gene            6325..7116
                     /gene="panB"
                     /locus_tag="WGR04_000923"
     CDS             6325..7116
                     /gene="panB"
                     /locus_tag="WGR04_000923"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ0794503.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRKSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVLAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMQGHDSVQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            7239..8075
                     /locus_tag="WGR04_000924"
     CDS             7239..8075
                     /locus_tag="WGR04_000924"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ0794504.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLAAEGIAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLSRELKAVAESLAQGSLDYA
                     GLERQAADHLTAAGWVVDYVEIRRADTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            8240..10078
                     /locus_tag="WGR04_000925"
     CDS             8240..10078
                     /locus_tag="WGR04_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794505.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVGKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALRYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMFALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            10218..10799
                     /locus_tag="WGR04_000926"
     CDS             10218..10799
                     /locus_tag="WGR04_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ0794506.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPLVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGIPSETETPERCAARTR"
     gene            10809..11654
                     /gene="ispE"
                     /locus_tag="WGR04_000927"
     CDS             10809..11654
                     /gene="ispE"
                     /locus_tag="WGR04_000927"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ0794507.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVDGMPQEADLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCSRTTQQLIDLGATLGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRDSAYNIYRQVSGLYEAYLAEGLSKH
                     PLLSV"
     gene            11662..11737
                     /locus_tag="WGR04_000928"
     tRNA            11662..11737
                     /locus_tag="WGR04_000928"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11695..11697,aa:Gln,seq:ttg)
     gene            11750..11825
                     /locus_tag="WGR04_000929"
     tRNA            11750..11825
                     /locus_tag="WGR04_000929"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11783..11785,aa:Gln,seq:ttg)
     gene            11834..11909
                     /locus_tag="WGR04_000930"
     tRNA            11834..11909
                     /locus_tag="WGR04_000930"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11867..11869,aa:Gln,seq:ttg)
     gene            12076..13059
                     /locus_tag="WGR04_000931"
     CDS             12076..13059
                     /locus_tag="WGR04_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ0794508.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            13126..13698
                     /locus_tag="WGR04_000932"
     CDS             13126..13698
                     /locus_tag="WGR04_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ0794509.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQPVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(13823..14992)
                     /locus_tag="WGR04_000933"
     CDS             complement(13823..14992)
                     /locus_tag="WGR04_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ0794510.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNRPTAHAAPTLQTPETL
                     TAAHIVIDLQSRQTLSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLALLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVKKRSRWQRLWACLTGQ"
     gene            15140..16666
                     /gene="ilvA"
                     /locus_tag="WGR04_000934"
     CDS             15140..16666
                     /gene="ilvA"
                     /locus_tag="WGR04_000934"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ0794511.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(16723..17796)
                     /locus_tag="WGR04_000935"
     CDS             complement(16723..17796)
                     /locus_tag="WGR04_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ0794512.1"
                     /translation="MSITIQNLNKHFGAFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGQILFDDQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(17793..18653)
                     /gene="cysW"
                     /locus_tag="WGR04_000936"
     CDS             complement(17793..18653)
                     /gene="cysW"
                     /locus_tag="WGR04_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ0794513.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTIPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            complement(19021..19857)
                     /gene="cysT"
                     /locus_tag="WGR04_000937"
     CDS             complement(19021..19857)
                     /gene="cysT"
                     /locus_tag="WGR04_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ0794514.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            19991..20098
                     /locus_tag="WGR04_000938"
                     /pseudo
     CDS             19991..20098
                     /locus_tag="WGR04_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            20143..20475
                     /locus_tag="WGR04_000939"
     CDS             20143..20475
                     /locus_tag="WGR04_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ0794515.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GEACACGH"
     gene            20807..21316
                     /locus_tag="WGR04_000940"
     CDS             20807..21316
                     /locus_tag="WGR04_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ0794516.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT         5187 a         5020 c         5840 g         5482 t
ORIGIN      
        1 ctatacatct caatacgata aatatttctt aagtcaaaat gcaagcctga ccgtacctta
       61 actgtcaaaa ttttattatt ttttattgat tttaaaacaa tttctgcaaa attctcttcg
      121 ctttctctct tttttagaag cacataagaa aaaataaaac ttccccgatt aaattcataa
      181 atatgtttca accatttgcc tcctctttct gtaagacaag attcagtttc attcttcctt
      241 attgtataaa tatttccttc acaaaatctg aaataaatcc ataaatccat cttatccata
      301 attaaagaaa aagtttcacc tcgagatttt gtcaacaatt cgcaaggttg cgatgttgca
      361 atcaaatagc cgaaagacat tttttacctc atacatggtc gaaatcagtt tctgttagtt
      421 cagaatccat tttttcgtca acaactgaat ccgcattttt gaattaacgt tttcatcagc
      481 tgccgtttat ctaaaccggc aggttcagtt tcaaaataag ccttatatga agactgtaag
      541 catttcagaa aaagatcatc agaagacata tctgccgaat caaatacaac tgttttgatt
      601 ttggtactta cccaaaaccc tttttgctct ttttctacta tacggaaatt cagaatattt
      661 ccaaccgaat caaaagcacg gtaaacatca tccatcaaat cctgcggctc tattttcttt
      721 tccaattccg acaatccttg aaatatatcc aaagacacat cttcaaatag aaaaaaagga
      781 ggagttagaa gcggtttttc catgatctgt ccgtagattt tgattcccaa gggcgatgac
      841 gaccaattcc ctgtccaggc aaagtcttgc ccgtattatc cgtaactcga cgatgataat
      901 ggggaaattt tccaatagga tgacctgttc tattaccgaa aggggctatc cgcatattat
      961 tgccgatttt aatctcacgt ccatatttag caaaggaaac aacctttcct gcggcgccta
     1021 caccaccagg aattgcgcct aatccgccag caatagcaac atctctaaca gaagctggtc
     1081 tgcctgtcgt tgcataacta aaaccatgct gtgtccacat accaatggca gcaccaccca
     1141 agatagccaa tggaagaaac gccctctcag tctccttcat ctccttttga gaaagttccg
     1201 ccaactgcat cggcgcatct gcccgcgtat ggaatacttg gttttcaaat atctgattgt
     1261 ccatgctgtt tgccattgcg ggggcaatca tagacagcat cattacggct gcggtaattt
     1321 gttttttcat aataactcct ttggattaca aggttggaaa atcaaagccc tgcttagaac
     1381 gtatgttgca cacccaattt caataggtaa atcagattgc aaatccagca atttgaatat
     1441 tgtcattgtt ccgtgcaaaa gggattttta ttgatgagtt gtgtactggg tttcagcttg
     1501 gctttttaga taatcttttc acaagaacat acatcaatac tgtccaaaaa aaaagatttg
     1561 ataaatatat tgcaacatat tttattccac cttcatccga aacagaaacc aatatatttt
     1621 taattaatac atagctgatt attatattta ttattcctat aatatataag gatcttgcca
     1681 aaattttttt tgattttatt ttaggaatat ctcttatcca tgctaaaata cagcccaatg
     1741 tcgaaaagaa aataacagca aacactacct tacctcctct cacttccaac caaaccgata
     1801 gcaggtttgg ttggaagttg gtttatttat tatttaacgg cgaatgtcag tgttcttacc
     1861 cgtagaacct gcataaccat ttatgccacc agcaacagtc cccactgcta cacctcgcat
     1921 accattaatc agtgctcttc ctccgttaat agggttcatc gcaccaatta acgcaccacc
     1981 tccaattgct gtccaatgtt catttcgatt atacgagcca ctagcaatag cactagccat
     2041 atagctgaaa tgccctccaa ctcctcccat aatgcctcca acagcattag gaatccactc
     2101 cccctcagtc tccttcatct ccttttgaga aagctccgcc aactgcatcg gcgcatctgc
     2161 ctgcgtgtgg aacacttggt tttcaaatgt ctgattgtcc aagccgtttg ccattgcggg
     2221 ggcaatcata gacagcatca ttacggctga ggtgatttgt tttttcataa taactccttt
     2281 ggattacaag gttggaaaat caaaacctgc ttaaaatgta tgctgtacgc caaatttcag
     2341 ttcggaactg ctttgccctg aaacgttgaa acgtgcggat gcgtttaaag ccgtggtttt
     2401 ggtgaaaccg aaacctgtgc cgaaatgagc gtaggtggat gtgtttcttg cggtttcttt
     2461 tttgccgtct atgcggtcgg gctgtgtgcc cagccattgg atgcctccgg tcaggctgat
     2521 tctgtcgttg gcggcaaatg agatgttggg gttgagcagc aggtagttgc ccgatttgta
     2581 gcggatgccg tctgaaaggg ttttgctgcc gttgatgcgg taggcggcgg tgagggaaag
     2641 gacaatcgga tctatggctt tgtaggtggt ggcgccgatg agccaggatt ttcccgacga
     2701 ggctttgttg cgcgattttt cgtaaaccgt gctttcaaga aagctgatta gggcggggtt
     2761 tttgtcgtct ttaaggaaag tgtggctgat gccgagggat acgtcggaca tccgtttgtt
     2821 gcgggttttg ccgttgccgt cgagtttgcg ttcttcgtgc cacagatagc tgccgctgcc
     2881 gtaaatgtcg gtattcccgg tcagtccgta gcgcaaaccg agcgtgccga cgagcatatc
     2941 ggtattgctg ccgttttctt ggatttcggt cggaatgggg ataaacgagg ttgcgccggt
     3001 ttgaatgtaa accggtgcgg caagttcggc gcggttgttt tcgctgttca ggtaggtaag
     3061 ggaagtttcc agtttccatt ttcccttgtc ggtcattatg tcttcaatcg tcaagggcag
     3121 gtcggcataa gtggataaag gcaggatggc gggcaaggcg ggcaaaaaga tgcgcttcat
     3181 atttctccta ttgatattaa ttcttattta ctgataataa tgaatatccg ataatcttgt
     3241 ctaaataaat ttttaaatca taaaaaacaa tatgtttgtt tttgtcaatt atgtttcgat
     3301 atgccgtctg aaaagtttgt gaaaaacggt tacaatccgc cgcatgaaaa aacgcaataa
     3361 tcctcttccg ctgttgaacg gtgtcaaacc cagttatttg gtgctgccgc atgaaaagca
     3421 gttttacggg ctgccgctgc tgcattttct gtgcatccgc tttccttttg tgggcgcgga
     3481 cgattggcgc aggcggttga acagcggttt tgtggtcggt tcggatggtg cggcgttgga
     3541 cgaacattct ttgttcgagc cgggtaaggt gatgttttat taccgtgaaa ccagccgtga
     3601 gagcgagccg cgtattccgt ttgaagaaaa gattttgcat attgatgagc atttgattgt
     3661 ggtggacaaa ccgcattttc tgcccgtcat ccccagcggc aggtttttgc gggaaaccct
     3721 gctcacgcgc ctgcgtttgc ggcctgaatt gcagcatttg aatgttgagg acattacgcc
     3781 gctgcaccgc ttggacaagg atacggcagg cgtgatgctg ctgtcgcaca atcctgccac
     3841 gcgcggagcc tatcagacga tgtttcaaaa caaaacggta tggaaaacgt atgaggcgct
     3901 tgcgccgaca aggacggatt tgccgtatcc gctcgatgtg gtttcgcgtt tggtgagggg
     3961 tgagaaattt tttacgacgc aagaggcgga aggcgaacca aacgcacata cgacggtcga
     4021 actgattgaa aacagggggg aattcagcct ttaccgcctt acgccgcata cgggcaagaa
     4081 acaccagttg cgcgtgcata tgatgggttt gggtatgccg ctgttgaatg acgcgctcta
     4141 tcccgtgccg tctgaagcgg gcagcgagga ttatcggaaa cctttgaagc tattggcaaa
     4201 aaagattgcg tttgcagatc ctttgtcggg tcgggaaagg gtgttttgca gcggattttg
     4261 cctataatga gagggaacgc aaacccgtat cggcggatgc cgccgatacg ggttttacat
     4321 tatcgatgcc gtctgaaagg ggttttatcc gttcaggcat acgggcgcga agcggaggat
     4381 ggtgtcgcgc aggacgatga gtttgccgtg aaagaaatgc gtggaaccgg cgatggtaat
     4441 gacgggcaaa tcttgcggtt ccgcccattt cagtgctttc ccgatttcga cgacttcgtc
     4501 ttccgcgccg tgtatcatca gcgttttggc aacgttggga acggcggacg gttccggacg
     4561 gtcggtatag tggcagactg ccgcgccgat gagcagcaat aaatcgggtg tgcgcgcttg
     4621 tgcggcaaat gtggcgacat aaccgccgaa ggagaagccg gataaggcaa attcgggggc
     4681 ttcggggtgt tgggcgcggg cataatcgat gacggcgagg cagtcttgcg tttcgccgcg
     4741 tccgtaatca tgtgtgcctc cgctgccgcc tacgccgcga aggttgggca ggtagcagtg
     4801 gaagccgagt ttgcttaagg ctttggcggc agtttggatg actttgttgg tgtttgttcc
     4861 gccctggagg gggttgggat gattgatgac ggcaacaccg cgtgccggta cttgttcgga
     4921 cgggatatgg atggtttcta aaatgccggc aggaccggat atgtgtatgg tttcgggttt
     4981 cagcataatg atttcctctt gattttccgc atgttccgat gcagccgtga agatagttgc
     5041 ttaaaggtgg aaatgcttgc cgttgttggg gttggacgct ttcaggccgg caagcatacg
     5101 gtggaagtcc aatccgtatg cgttgctcag tttgtcggca cgttttttga gtttgtctat
     5161 gttttgctct ttggggctgc tttggaaggc cattaccgcg acattgccgt ggctttcggc
     5221 aggaagttcc aagacgcgcc cttcaaaaac gctcaacaac cgttcgatga agcgttggta
     5281 gcgtttgtcg ccgctccacc agttggttac gaatatgccg tctgaagaga gtgcgttgcg
     5341 gcagtctcgg aagaacggtt cttcaaccag cgcatcgatg atttgttcgc cgtcgaaccc
     5401 gtccaccaaa atcacatcgg tgttgtggcg gaagactttg atatattctg caccgtctgc
     5461 ttcaataatt tcaaatttct cgccctcgaa aggcaactcg aacaggttgc gggcaatggc
     5521 gatgacctgc ggattgatgt ccacggcggt ttggcgcgtg tcgggcaggt aggtatctat
     5581 ccagcgtgca aacgagccgc cgcccaagcc gatttgggtg atatgttgcg gaagagcgtc
     5641 ggtaaacagc agccagccca tcatcgcgcg gctgtaagag agcaccagct cggacgggtg
     5701 gtcgaggttc atcgagcttt gaacggtgtc gctgcccaag tgaagcgaac ggatattgcc
     5761 ttcttcggaa atgccgactt cgggaaagcc ggattttgca ggacgcaggc ggcggtaggg
     5821 atgtcttgcc atcggtgtga acggtcggtt tgaaaggcgg atattttatc agaatgccga
     5881 tgcttgttct gtttcaaatt aatttctttt caaataaatt acttattcgg atttgccggg
     5941 gctttcggat aaattccttg ccaaggtgcg gcattgcctg cataattcgc tttctttgcc
     6001 ggtatagctc agttggtaga gcacctgact tgtaatcagg gggtcccgag ttcgactctt
     6061 ggtgccggca ccatttctgc tgatggtgtt gcaaaacatt tcagtaagcc ggtatagctc
     6121 agttggtaga gcacctgact tgtaatcagg gggtcccgag ttcgactctt ggtgccggca
     6181 ccagatttga cagtccgatt gtgtaaaaca gtcgggctgt tttgcatttg cggcacggtc
     6241 ggtggcaggg ctgcgggcat ttcactataa tagcccgttt caaactgaaa aggccgtctg
     6301 aaaagggcgg ggtaacaata tctgatgatt actgtgaaca cactgcaaaa aatgaaggcg
     6361 gcgggcgaaa aaatcgctat gctgaccgct tacgaatcca gttttgccgc gctgatggac
     6421 gatgccggcg tggaaatgct gctggtcggc gattctttgg ggatggcggt tcaggggcgg
     6481 aaatcgacgc tgcccgtcag cctgcgcgat atgtgttatc acaccgaatg tgtagcacgc
     6541 ggtgcaaaaa atgcgatgat tgtcagcgat ttgccgtttg gtgcatatca gcagagtaag
     6601 gagcaggcgt ttgccgccgc cgccgaactg atggctgccg gcgcgcatat ggttaaactc
     6661 gaaggcggcg tgtggatggc ggaaacgact gaatttttgc aaatgcgcgg tattccggtt
     6721 tgtgcgcaca tcggtctgac cccgcaatcc gtgtttgcct tcggcggata taaagttcag
     6781 gggcgcggcg gcaaggcgca ggcgttgctt aacgatgcca aggcgcatga cgatgcgggg
     6841 gcggcggtcg tgctgatgga gtgcgtactg gcggaactgg caaaaaaggt aactgaaact
     6901 gtttcctgtc cgaccatcgg catcggcgcg ggtgcggatt gcgacgggca ggttttggtg
     6961 atgcacgata tgctcggcat tttcccgggt aagacggcga aattcgtcaa aaactttatg
     7021 caggggcatg atagtgttca agcggcggtt cgggcgtatg ttgccgaagt caaagccaaa
     7081 accttccctg ctgcggaaca tatttttgca gattgagcgg tttacatgca gcatgccgtc
     7141 tgaaagccgt ttcagacggc attttgtttt gccgtgcgcg gcgcgtataa tcggcgcgtt
     7201 ttgtcgggca ggaagcccga aggataagga ttaccgttat gcaaatcata cataccattc
     7261 gagaactgcg cgcgtggcgt aaaaatgcgg gaaaggtggc atttgtgccg accatgggca
     7321 atctgcatga aggacatctt gcgcttgtgc gtgaggcgaa aaaacgcgcg gacagtgtcg
     7381 tggtcagcat tttcgtcaat cgcctgcaat tcggtcaggg cgaggatttc gacaaatatc
     7441 cgcgcacttt gcaacaggat gcggacaaac ttgccgccga aggcattgcc gttgttttcg
     7501 cgcccgatga gaaagaactc tatccgaacg tggaacagcg ttacaacgtc gaaccgccca
     7561 atctgcaaaa tgagttgtgc ggcaaattcc gcccggggca ttttcgcggt gtggcaacgg
     7621 tagtgtccaa gctgttcaat attgtcgcgc cggatgttgc ctgtttcggc aaaaaagact
     7681 atcagcagct tgccgtgatt aaaggtttga ccgaggattt gaattttgat gttgaaatag
     7741 tgcctgtcga tacggggcgc gcggaagacg gtttggcact ctccagccgc aaccagtatt
     7801 tgagtgcggc ggaacgcgac gaagcaccgc gcctgtcccg cgaattaaag gctgttgccg
     7861 aatccttggc gcagggcagt ttggattatg caggtttgga gcggcaggct gccgaccatt
     7921 tgaccgctgc gggctgggtg gtcgattatg tcgaaatccg ccgcgccgat acgctcgaag
     7981 tggcgcgggc gggagataag aaactggtgg tcttggccgc cgcccgtctg gggacgacgc
     8041 gcctgattga caatttggaa ataaaactcc cttaaaccgc aagcgtcggg aatgccgtct
     8101 gaagcggatt tgcgtttcag acggcattta tttttttgaa cggggtttcg caaatctaca
     8161 aacgattctg cttgtgataa agttacgcct gattatatgc cgtctgaagg ttcggacggc
     8221 tgtcggataa aggaagatta tgttacccgc ccgtttcacc attttatctg tgctcgcggc
     8281 agccctgctt gccgggcagg cgtatgccgc cggcgcggcg gatgcgaagc cgccgaagga
     8341 agtcggaaag gttttcagaa agcagcagcg ttacagcgag gaagaaatca aaaacgaacg
     8401 cgcacggctt gcggcagtgg gcgagcgggt taatcagata tttacgttgc tgggagggga
     8461 aaccgccttg caaaaggggc aggcgggaac ggctctggca acctatatgc tgatgttgga
     8521 acgcacaaaa tcccccgaag tcgccgaacg cgccttggaa atggccgtgt cgctgaacgc
     8581 gtttgaacag gcggaaatga tttatcagaa atggcggcag attgagccta taccgggtaa
     8641 ggcgcaaaaa cgggcggggt ggctgcggaa cgtgctgagg gaaagaggaa atcagcatct
     8701 agacggactg gaagaagtgc tggctcaggc ggacgaagga cagaaccgca gggtgttttt
     8761 attgttggca caagccgccg tgcaacagga cgggttggcg caaaaagcat cgaaagcggt
     8821 tcgccgcgcg gcgttgagat atgaacatct gcccgaagcg gcggttgccg atgtggtgtt
     8881 cagcgtacag ggacgcgaaa aggaaaaggc aatcggagct ttgcagcgtt tggcgaagct
     8941 cgatacggaa atattgcccc ccactttaat gacgttgcgt ctgactgcac gcaaatatcc
     9001 cgaaatactc gacggctttt tcgagcagac agacacccaa aacctttcgg ccgtctggca
     9061 ggaaatggaa attatgaatc tggtttccct gcacaggctg gatgatgcct atgcgcgttt
     9121 gaacgtgctg ttggaacgca atccgaatgc agacctgtat attcaggcag cgatattggc
     9181 ggcaaaccga aaagaaggtg cttccgttat cgacggctac gccgaaaagg catacggcag
     9241 ggggacgggg gaacagcggg gcagggcggc aatgacggcg gcgatgatat atgccgaccg
     9301 aagggattac accaaagtca ggcagtggtt gaaaaaagtg tccgctccgg aatacctgtt
     9361 cgacaaaggt gtgctggcgg ctgcggcggc tgtcgagttg gacggcggca gggcggcttt
     9421 gcggcagatc ggcagggtgc ggaaacttcc cgaacagcag gggcggtatt ttacggcaga
     9481 caatttgtcc aaaatacaga tgttcgccct gtcgaagctg cccgacaaac gggaggcttt
     9541 gagggggttg gacaagatta tcgaaaaacc gcctgccggc agtaatacag agttacaggc
     9601 agaggcattg gtacagcggt cagttgttta cgatcggctt ggcaagcgga aaaaaatgat
     9661 ttcagatctt gaaagggcgt tcaggcttgc acccgataac gctcagatta tgaataatct
     9721 gggctacagc ctgctttccg attccaaacg tttggacgaa ggcttcgccc tgcttcagac
     9781 ggcataccaa atcaacccgg acgataccgc tgtcaacgac agcataggct gggcgtatta
     9841 cctgaaaggc gacgcggaaa gcgcgctgcc gtatctgcgg tattcgtttg aaaacgaccc
     9901 cgagcccgaa gttgccgccc atttgggcga agtgttgtgg gcattgggcg aacgcgatca
     9961 ggcggttgac gtatggacgc aggcggcaca ccttacggga gacaagaaaa tatggcggga
    10021 aacgctcaaa cgtcacggca tcgcattgcc ccaaccttcc cgcaaacccc ggaaataatg
    10081 caggtccatc ctttcagacg gcataaggtt tgccgggaag ctgcggcatt cgggcaaacc
    10141 gcacgcagtt cgcacgcgtt ttgcacggca cgccgaaccc gccggccggc aggatggcac
    10201 ccgttaagga aattctgatg aaacacaccg tatccgcatc ggtcatcctg cttttgaccg
    10261 cttgtgcgca attacctcaa aataacgaaa acctgtggca gccgtccgaa cacatcagca
    10321 gttttgcagc agaagggcgg ttggcagtga aagcggaagg gaaaggttcg tatgcaaatt
    10381 tcgattggac ataccaaccg ctcgtggaaa ccatcaatat caatacccct ttgggcagta
    10441 cgctcgggca gttgtgtcaa gacagggacg gcgcattggc agtggacggc aaaggaaatg
    10501 tctatcaggc ggaaagtgcg gaagaattga gcaggcagct ggtcggtttc aaactgccaa
    10561 tccaatatct gcatatctgg gcagatggca ggcgtgtggc gggcgcgcct taccgcatcc
    10621 tgccggacgg catattggaa caatacggtt ggactgtcgg cagaaccgcc gacagtgggg
    10681 ggcaagtccg aacgttgcaa ctgaataacg gaaatttgaa catcaggctg gttttcaccg
    10741 aaatcggtat tccgtctgaa accgaaaccc cggaacgctg tgcggcgcgc acgagataag
    10801 gcggacagat gaatattgcg gacggacggc aggcgtttcc cgcacctgca aaactgaatc
    10861 tcgatttgag gattaccggc aggcgggaag acggttatca caatatcgaa agcatattct
    10921 gcttgataga tttgcaggat accgtatatt tgaaaccgag ggacgacggc aaaatcatcc
    10981 tgcacaatcc ggttgatggc atgccgcagg aagccgattt gagctaccgt gccgcatcgt
    11041 tgctgcaaaa atatgcgcgc aaccccgccg gcgtggaaat atggctggac aaaaaaatcc
    11101 cgacaggggc gggtttgggc ggcggaagct cggatgcggc aaccgttttg ctggtgttga
    11161 accgttggtg gcagtgttcc cgaacgacgc agcagctgat tgacttgggc gcaactttgg
    11221 gtgcggacgt accatttttt attttcggaa aaaatgcttt tgcaagcggg ataggtgaaa
    11281 aactgatagg aatggatatt ccgaaacagt ggtatgtcat cgtcaaaccg cccgtccacg
    11341 tttccactgc aaaaattttc acacacgaag gcttgacacg aaattccgcc tcaagcataa
    11401 tgccgacttt ccaaaacctg caaccgttta gaaatgatat gcaggcagtt gtatttaaag
    11461 aataccctga agtttggaaa gcctattccg aattgtcccg atatggattt gccttaatga
    11521 caggttctgg tgcgtgtgta ttcacggcgt gtcaagatag ggatagcgca tacaatatat
    11581 accgacaagt ttcaggtttg tacgaggcat atttggcaga gggtctttca aaacatcctt
    11641 tgttgtccgt ataaacattg ttggggagtc gtcaagcggt taagacactg gattttgatt
    11701 ccagcatgcg aaggttcgaa tccttcctcc ccagccaagc caaacgagtt ggggagtcgt
    11761 caagcggtta agacactgga ttttgattcc agcatgcgaa ggttcgaatc cttcctcccc
    11821 agccagccct ttttggggag tcgtcaagcg gttaagacac tggattttga ttccagcatg
    11881 cgaaggttcg aatccttcct ccccagccaa ataaaagcgt gtaagcctgc ttacacgctt
    11941 ttatttcata gaaataaaaa tattgaaatg cctttgtttg tgtcggatgt tgcaggtata
    12001 atgtcgggct tggtacaagc agagggaagc attgtgtttt ctgagcggaa gttaaacata
    12061 aaatcaggtg agaatatggc tgcgtacgac agtttgatgg tatttacagg caatgccaat
    12121 cccgaattgg cacaacgtgt tgtcaggcat ttggacattt ctttgggcaa tgcttccgta
    12181 tccaagtttt cagacggcga agttgccgtc gaactgttgg aaaacgtacg cgggcgcgat
    12241 gttttcatcc ttcagccgac ctgtgcgccg accaatgaca acctgatgga aatcctgacg
    12301 atggcggatg cactgaagcg tgcttcggca ggtcgtatta ccacagccat tccgtatttc
    12361 ggctatgcgc gccaagaccg ccgtccgcgt tccgtccgcg ttccgatttc tgccaaactg
    12421 gtggcaaata tgctgtattc ggcagggatc gaccgtgttt tgactgtcga tttgcatgcc
    12481 gaccagattc aaggtttctt cgatattccg gtggacaata tttatgccac cccgattctg
    12541 ttgaacgaca tcaaacaaca gcggattgaa aatctgaccg tcgtcagccc ggacatcggc
    12601 ggtgtcgtcc gcgcccgcgc cgtggcaaaa tccctgaatg ccgacttggc aatcatcgac
    12661 aaacgccgcc cgaaagccaa tgtggcggaa gtcatgaaca tcatcggcga tattcaaggt
    12721 agaacctgtc tgattgtgga cgatatgatt gacactgcaa atacgctgtg caaagccgcc
    12781 gtcgccctga aagagcgggg ggctgaacgt gttctagcct atgccagcca cgccgtattc
    12841 tccggagagg cggtcagccg tatcgcctca tccgaaatcg accaggtggt cgtaaccgat
    12901 accattcctt tgtctgaagc ggctaaaaac tgcgaccgta tccgtcaggt aacgattgcc
    12961 ggtctgttgg ccgaaaccgt ccgccgcatt agcaatgaag aatccgtctc atatcttttc
    13021 aatgaagaag tgatgacagg cagcatgttg ctgccataag cccgaagccg tcttaagctg
    13081 gtcgcggccg atgacggcga ttttacctaa cttggagtat ttaacatgac ttatgaaatt
    13141 caagcctctg ttcgtgaagc acaaggcact ggtgcgagcc gccgcctgcg tcgcgaaggc
    13201 caaatccccg gcattctgta cggtgaaggt caagagcctg ttgcaatcgc tgtggatcac
    13261 aaaaccgtat tctacgcatt ggaaaaagaa tctttccata ctgcgttgat taagttgtct
    13321 ctgaacggtg aaaccaaaga cgttatcgtc cgcgatttcc aaatgcaccc gttccgccgc
    13381 gaagttcaac acatcgactt ccaagctgtg aaagcagacc aacccgtacg catccgcgtt
    13441 cccctgcaca tcgttaacgc tgaaaattcc caagcggtca aactgcaagg cggccgcgta
    13501 tctctgttaa acacttctgt tgaagtagtt gctttgcctg ccaacatccc tgctttcttg
    13561 gatttggatt gtgctgaagt ggttgccggc gacattctgc acttgtcaga catcaaactg
    13621 cctgaaggtg tagaaagcgt ttccctgaaa cgtaacgaaa atctggctgt tgctaccgtt
    13681 accggtaaga aacgctaatt gatttcagca gcagggcggc gcgtatgcaa tacgtaccgc
    13741 cctgttgttt tatgccgtct gaaccgtgtt tcagacggca tttctttatt tgttggaaaa
    13801 acgggatatt tgaaacggca gattactgcc ctgtcagaca cgcccaaagc ctttgccacc
    13861 ggcttctttt ttttacattt tccagtgcga cgatttcttt ttcggcaatg gtgtatccgt
    13921 tttgtctgat tttgattttt cctaaaattt gccctttttt tactggggcg ggaatcggct
    13981 gtatggtttc tagaatttgt tctgccattt tcgcttcctt atgtggcaga gtgatgtagg
    14041 cttctttgag gaagcctgcg cggacggttt ttttgctgcc tccggaaatt tggatttggg
    14101 caacggtttt gcctttcgga tatattttgg gcgtatcgaa ggcctgcaat gcccagttca
    14161 gcagcttgct gttgtctgat gcgcgtgttt ccgccgattc cgaacccaat gtgatgacaa
    14221 ggatgtgcct gccgttgccg gagtatgaca cggcaaggtt gtagccgccg ctttctgtgt
    14281 gtccggcttt cagaccgttt acattgttgt ccctatataa aaggatattg cggttgtttt
    14341 gttctatatt tttgaatttg aaagatttga tggaaaacag cgggtaatat tccggaaagt
    14401 cgcgcatcaa tgcttcagac agcagggcga ggtctttggc ggtggaaacc tgtccttctc
    14461 tactcaagcc tgtcgggttt ttgaatacag tgttcttcat gcccaagcgt cgggcttctt
    14521 tgttcatttg ttgcacaaaa ttttcaatcg agccgttgcc cagccggccg gcaagggtta
    14581 gggcggcatc gtttgcggat agtgcaatca tgccttttaa gagtttgtcg gtgctgaccg
    14641 tatcgccggg acgtacaaac attctgcttc cttctgaagc ccatgcggat tcgggtattt
    14701 ttaagttttc ttcagatcgg atatttcccg atttcatgtt tttgaaaacc aaatatgcgg
    14761 tcatcagttg ggttagtgcc gccggttcga caggggtatt gatgtttttg gcggataaag
    14821 tctgtctgct ttgaaggtcg ataacgatgt gtgccgctgt gagggtttcg ggtgtttgga
    14881 gcgtgggggc ggcgtgtgcc gtcggtctgt tgggcgcggg cgatgcagcc gttgcatgag
    14941 aaacgcctaa gatgatggaa agcaggacgg gcaggatttt atgtgctgtc atgaaatatt
    15001 ctaattgtgt gcgtgtttca gtctgccgat tatacgctta gggtgtctga tcgggcggat
    15061 ttttcttgct ttcgcgccgt cttgggcgta tggttttggg ttttgcgatt ttaataaacc
    15121 gattatccca tattgaatta tgaacacgcc ccttccttat tccaattacc tcatccgcat
    15181 cctgacggca tctgtctatg atgtcgcggt cgaaacgcct ttggaaccgg cacgcagcct
    15241 ttctgtacgt ttgaaaaaca acatcctttt gaaacgcgaa gatttgcaac cggttttttc
    15301 gttcaaaata cgcggcgcgt acaacaaaat gtccaagttg ccgaaagatg cgctcgcttg
    15361 cggcgtgatt gcggcaagcg cgggcaatca cgcacaaggc gtggcattgt ccgcacagcg
    15421 tttgggttgc cgtgccgtta tcgtcatgcc ggagactacg ccgaaaatca aagtggatgc
    15481 ggtcaaaagc cgtggcggcg aggtggtttt gcgcggtgtt tcatacaacg atgcttacga
    15541 ttatgcgatg gagctggcgg aacaggaagg gctgacctat atcgcaccgt ttgacgatcc
    15601 tgatgtgatt gcaggacaag ggacggtagg gatggaaatt gtcagccagc atcccgatcc
    15661 aatccgcgcc gtattcgtac cgataggcgg tggcggtttg gcggcgggcg tggcggcatt
    15721 tatcaagcag gtccgtcccg aaatcaaagt tatcggcgtt cagaccaacg attcctgctg
    15781 tatgaagcag tcggtcgaag cgggtgaaat cgtccatttg aaagatgtcg ggctgttttc
    15841 agacggcacg gcggtcaaag tcgtcggaaa cgaaaccttc cgcctctgca aagaactttt
    15901 ggatgaaatc attacagtcg ataccgatgc ggtttgcggc gcggtcaagg atattttcga
    15961 tgacacgcgc agcattaccg agccggcggg cgcgttggcg ttggcgggtc tgaaagccta
    16021 tatcgcccga gaaggcgcgg aaaaccaaac cctgattgcc gttaccagcg gtgcgaatat
    16081 gaattttcac cgtttgcgcc acgtttcgga acggagcgaa ttgggcgagg gcaacgaagg
    16141 tatttttgcc gttaccatcc ctgaagaacg cggcagcttc cttaagtttg tcaatatatt
    16201 gggaaatagg aatattaccg agttcaacta ccgctacggg gacgatgaaa aagcgcatat
    16261 ctttgtcgga cttcaagcgg caggcccgca ggatttggcg gttatcggca gccggttgga
    16321 tgaggcggga ttgcccaatg tcgacctgac cgatgatgag attgccaaaa tccatatccg
    16381 ctatatggtc ggagggcgga cggacaaagt agaaaacgag cgtctggtca gttttgagtt
    16441 tccggagcgt ccgggggcgt tggcgcgttt tttgaaccat atgcagggcg gttggaacat
    16501 tacgctcttc cattaccgca accacggtgc ggattacggg cggattttgg tcggtatcga
    16561 tgtgccgccg cacgatgccg ccgcatttga cggtttcttg gaaagtctgg gatacagcta
    16621 tcacgaggaa acgcaaaatg ccgcgtacaa gctgtttctt gcctgacgct tgaaagcaca
    16681 atgccgtctg aaagcctttc agacggcatt gcgctttcat ggttaaatcg aatattcaat
    16741 cagttcgttt tgagagaaga cataaacctg tttcggaatc agcttcaatt ctttgccttc
    16801 ggcgattggg taacgcgcgg catcgctgcc agccagcgtg atatgcacgt cctgtttgcc
    16861 gtgtttcacc aaaatatgcg tcaatgcccc gacggcgtgg actttttcga tttcggcaca
    16921 aatcatcggt gtttcgtgtt cggcggcgat ctgccattcg tgcgggcgga tatagccggt
    16981 ggcggtttgt tcctgccatt tgtattgtgc gtccaatttc cacgcgaagc cgttataatg
    17041 ccagaagcct ttttcgatgc gtccttcaaa agcgtcggtt tcgcctaaga actcggtaac
    17101 gaaggcattt tcgggtttgc ggtaaatggc ttcggcactg ccggtttgtt cgattttgcc
    17161 gtggttcatc acgacgattt cgtcggaaac ttcgagggct tcttcttggt cgtgtgtaac
    17221 caaaatgctg gttacgccga ggttgtgatg gatgtcgcgc aaccaggtgc gtaattcttt
    17281 gcgtactttg gcatccagcg cgccgaaggg ttcgtccaaa agcaggagtt tcggttcgac
    17341 cgcaagcgca cgggcgaggg cgatgcgctg gcgttgcccg ccggagagtt ggtgcggata
    17401 ggattttgcc aaatgcgaga gttgtacgag tttcagcaat tcttcgactt tggcgcggat
    17461 ttgtcctttg gacgggcgtt cggacttggg caatacggtc aaaccgaagg cgacgttgtc
    17521 aaacacgttc atgtggcgga agagggcgta gtgttggaac acgaagccga ctttacgctc
    17581 gcgtacatgc ttggcagtta cgtcttggtc gtcaaagaga atttggccgc cgtcggcgtt
    17641 ttccagtccg gcgatgatgc gcaggagcgt ggttttgccg cagccggaag ggccgagcag
    17701 ggaaacgagt ttgccggtgg ggacgttgag attgatgttt ttcagcgcgt ggaacgctcc
    17761 gaagtgtttg tttaagtttt gaatggtgat gctcatactg cgttcctttc ggcggcggcg
    17821 agttttttgt cttgtaattt ggtgatgatg ttctgcaccg ccagcgtcgc cagtgctaaa
    17881 agtgccaata cgccggagag ggcgaatgcg ccggtgaagt tgtattcgtt gtagaagatt
    17941 tcgaccaaaa gcggaatggt gttggtttcg ccgcgtatgt gtcccgatac cacgctgacc
    18001 gcgccgaact cgcccatcgc gcgggcgttg gtgaggatga tgccgtagag taacgcccat
    18061 ttgatgttgg gcagggtaac gcgccaaaac atctgccagc cgcttgcgcc gagtatcaat
    18121 gccgcctgtt cttcgctgtc gccctgagcc tgcatcagcg ggatgatttc gcgtgcgaca
    18181 aaggggaaag taacgaacag cgtcgccaga ataatgccgg ggatggcgaa gataatctgt
    18241 atgccttgcg cttcgagcca gccacccaat gccgtgtgcg cgccgaacaa taagacgaac
    18301 attaaaccgg ccaccacggg cgatacggaa aacggcaaat cgagcagggt agtcagcaac
    18361 tgcttgcctc ggaagtcaaa acgggtcagc agccacgcca tcgccacacc caatacagca
    18421 ttgacgggaa cgacaatcag cgcggtaatc agcgtcaatt tgatggcaga ccacgcttcg
    18481 ggatcgttta aggattttag gtacaaatcc caaccgcctt ttaaggcttc gtaaaacacg
    18541 gcgacgagcg gcacgaccag catcagcagc agaaagccca gcgcggcggc aatcagcaac
    18601 acgcgcagcc ggcgcggttc ggtcaggttg ggattggcgg aatagggttt catggcggtg
    18661 gtgttctctg gttggtttaa aatgtttgga ggtcgtctga aaagggattg aggggtttca
    18721 gacgaccttt ttgttcatta agtagtaggt cggatacttg tatccgacaa ggttgctgaa
    18781 tcgcaaatgt ttgtcggata caagtatccg acctacgaat tctgtgtttt gtggggcgga
    18841 ttcgttttca gacaaccttt tattgattaa gggaataaaa acgtctagct accgtcattc
    18901 ccgcgcaggc gggaatccac cgcagggcaa caggaaaaca gaaaataaat aaggcagctg
    18961 aaattcacca atggattccc gcctgcgcgg gaatgacggt aacaggtatt tcagacgacc
    19021 tcaacccttc gcgcccgaac gcctgcccaa cgcccactgc atcacgttca gcgcaaacag
    19081 aatcacaaac gaaaccagca gcataaacaa cgccaccgcc gacgcgccct gtacgtcgaa
    19141 ctgttccagc ttgcccgtaa taatcagcgg caggatttca gaaaccatcg gaatattgcc
    19201 cgcgataaaa atcaccgagc cgtattcccc cgttgcccgc gcgaacatca ttcccgcgcc
    19261 ggtcaagagt gccggcgtga tttccggcag cagcacgcgg cggaacgtcg taaagcggtt
    19321 tgcgcccaaa gtcgccgccg cttcctcata ttcgcccgat aattcctcca acaccggctg
    19381 cacggcgcgg acgataaagg gcaggctgac gacgaccagc gcaatccaaa tgccgacggg
    19441 cgtaaacgcg attttgatgc ccaaaggctc gaaaaaacgg cctatccaac cgttgggcgc
    19501 atacagggtc gccaacgcga tacccgtaac cgccgtcggc agcgcaaacg gcaaatcgac
    19561 cagcgcgttc gccagaccct tgcccgggaa ttcataacgc accaataccc acgccaccag
    19621 cgtgccgaac acgacattgg tcagcatcgc ataaaacgac atccgcaaac tcagccacac
    19681 cgccgccaac acgttcggct cggcaatcgt gttccaaaag ccgccccagc cgatttccgc
    19741 cgccttcgcc gccatcatcg caaacggcaa gaccacaagc agcgacaggc acaatacggt
    19801 cagaccgagg ctgagtttga aaccgggaag gacgccgggt gttttgagcg gtaacatggt
    19861 cggtaagggt ggaaaataaa ggcattactt taacgatgcc gtctgaaaaa cggaaagaat
    19921 ggaaagtttg gtgcaaagac gaatttgtta taaagcggtt ggcagtttct caagcgggcg
    19981 cgatgtttta aaatatagtg gattaaattt aaatcaggat aaggcgacga agccgcagac
    20041 agtacaaata gtacggcaag gcgaggcaac gccgtactgg tttaaattta atccactata
    20101 acgccttgtt ttgacggaaa accatcatat aaaggaacac ttatgcagat tttatctttt
    20161 cagccggaca ttgcggaacg tatgctggaa ggtacggaag gcgagtcggt caacgaaaac
    20221 gcacaattcg tccgtacgga caacggctat tggattgcgt ggcatgaagg cgtagcggca
    20281 ctgcttgcgc ccgatacgcc gccgggcatt ccctgttttt gggtggaagg cgcggaaagc
    20341 cttgaagagt tgtgcgtcat ggtggaacgc ggcgagtttg acgaagtgga agagtttgac
    20401 ggcgatgacg acgaatggct cgaaacggca cagggttgcg ggcaccacgg cgaagcttgc
    20461 gcctgcggac attaaaggca ttgcaggctt gccgcaaggg gggcaaggct ttgccgtttt
    20521 ttaaataatc ggtggattgc atctgtttgc aatgggctga acgcttccct ttgtatttta
    20581 ttgaataaaa aaacttatct tggtattata attaaggcag catcaattat tttgggatgg
    20641 caataaacgc aaagcattga tttgcgccga ttgcagactt attatagcag gttgcggcgc
    20701 ggacttaatg ggaattgttt atagagcatg atggtctgaa acatcattaa ttaaaaatac
    20761 cattaatccg atttatattt atttcaattt caatggaaaa acatcaatga caatgatttt
    20821 aagcatttta agcctgtttt ttatcatcag actgttattt ttagccgtct ctattaaaca
    20881 tgaaaaagcc ttgattgcca aaggggcgaa acaatacgga aaaaccaatt ccacgctgct
    20941 tgcggcagtt catacgcttt attatttggc gtgttttgtt tgggtatggc tttctgacac
    21001 tgcttttaat ggcatatcct tgattggtac gctgacggtg atggcttcat ttgtgatatt
    21061 gtcattgatt attaagcagt tgggggagat ttggacggtt aaaatctata ttttaccaaa
    21121 tcatcaaatt aatcgttcgt ggttgtttaa aacattccgc caccccaatt attttttaaa
    21181 catcataccc gaactgattg gcatcgcctt attatgtcaa gcgtggtatg ttttattgat
    21241 tggtctgccc atttatttgc tggtcttatt taagcgtatc cgacaagaag aacaggcgat
    21301 ggcaacactt ttttaacccg tttcatcaat tatagtggat taacaaaaac cagtacggcg
    21361 ttgcctcgcc ttgccgtact ggtttttgtt aatccactat attccgccat ctctaagatt
    21421 tacagcgata cacgggtgat ttaaggaatg cccgaaccgt cattcccgcc acttttcgtc
    21481 attcccgcga aagcgggaat ctaggacgca gggttaagaa aacctacat
//
LOCUS       DAWFJR010000027        20771 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWFJR010000027 DAWFJR010000000
VERSION     DAWFJR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20771
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.027"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(84..1130)
                     /gene="recA"
                     /locus_tag="WGR04_000941"
     CDS             complement(84..1130)
                     /gene="recA"
                     /locus_tag="WGR04_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ0794517.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGVDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIVNKSGAWYSYNGAKIGQGKDNVRVWLKENPEVANEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            1384..2148
                     /locus_tag="WGR04_000942"
     CDS             1384..2148
                     /locus_tag="WGR04_000942"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ0794518.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEILHHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFKLL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKELAAKPIVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLENGAD"
     gene            2168..4183
                     /gene="rep"
                     /locus_tag="WGR04_000943"
     CDS             2168..4183
                     /gene="rep"
                     /locus_tag="WGR04_000943"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ0794519.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSKLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLLASEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLNNLKKSGAAD"
     gene            complement(4251..5309)
                     /gene="dinB"
                     /locus_tag="WGR04_000944"
     CDS             complement(4251..5309)
                     /gene="dinB"
                     /locus_tag="WGR04_000944"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ0794520.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
     gene            complement(5362..5463)
                     /locus_tag="WGR04_000945"
                     /pseudo
     CDS             complement(5362..5463)
                     /locus_tag="WGR04_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(5557..8646)
                     /locus_tag="WGR04_000946"
     CDS             complement(5557..8646)
                     /locus_tag="WGR04_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0794521.1"
                     /translation="MNQNHFSLKILTVMLLSAYGGSFADGVVPVSDGNTVSLDTVNVR
                     GSHALSGKTEKTRSYTIDRMSTATGMRIAGKDTPQSVSVITRSRLDDKAVHTLEEAMK
                     NTTGVNVVRDSGLQTRFLSRGFYIDQIGEDGITVNVAGRSGYTAKIDVSPSTDLAVYD
                     HIEVVRGATGLTQSNSEPGGTVNLIRKRPTASFKHTGELTADHRGSRRAVLDVSGSLN
                     KANTLRGRLVGAEEYKKSFKDRVWGRKHMVYGIAEADAGDSSVLTLGGMYQKSREVPD
                     FSGIILSCENQKTAPFSSTPACNRPLQLPRNTYLGEDWSRLSADKYNLFSGFKHVFDN
                     GWQLNAEVSYTKNESDAKVGQFFLKNEHAAGLSDEDAVGFLTEKNEVIPFEPKDKALE
                     KLKAYRDETAKEYRERKDDFVKNRFDNTAFEQYRSRRAAERKAGFDECMSAPFALDFI
                     CQGSWGDLGVDADKSEFVDKALAKEGIFNNAAQRFPNSLYDSSFNRKATANRRYSYMP
                     LRHTKDDRQWGIKLDLTGTYGLFGREHDFFVGYAYGDEKIRSEYLEIYERRHRVRPNT
                     GATHGVYAGSCQEEPDGDLSSPLVRGHKEPDWQAYDEKGNRTVYAEECRNAKKIKTEP
                     KLDAEGKQVYYYDEYSGSRTPVYVDVYELDEKGNKIQETNPDGSPVFTGFSGTVPVWK
                     TVKVADDHVPALYNYAKYLNTNKTHSLTASTRFNVTGRLHLLGGLHYTRYETSQTKDM
                     PVRYGQPASDFQTASSIKADQDHYTAKMQGHKLMPYAGITYDLTPQQSIYGSYTKIFK
                     QQDNVDVSAKTVLPPLVGTNYEVGWKGAFLQGRLNASFALFYLEQKNRTVVDFGYVPR
                     AGGKQGSFQTVAKPIGKVVSRGAEFELSGELNEDWKVFAGYTYNKSRYKNAAEVNAER
                     LAKNTGADPYNFSNFTPVHIFRFGTSFHIPNTGLTVGGGVSAQSGTSSLYNIRQGGYG
                     LIDGFVRYELGKHAKLSLIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMKLDWQF"
     gene            8920..9699
                     /locus_tag="WGR04_000947"
     CDS             8920..9699
                     /locus_tag="WGR04_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0794522.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYGHSFHFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            9712..9852
                     /locus_tag="WGR04_000948"
     CDS             9712..9852
                     /locus_tag="WGR04_000948"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794523.1"
                     /translation="MSDAFGWTVKPTRHENRVGKNAVWKNSDGIFGHITCKRQHIPTQ
                     TD"
     gene            complement(9910..11322)
                     /locus_tag="WGR04_000949"
     CDS             complement(9910..11322)
                     /locus_tag="WGR04_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0794524.1"
                     /translation="MLFEFKENKMIVASRWPLLEKAFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFP
                     ALGSLHAYKIKAELVGRYHSGCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVT
                     DNPPTGLFANKKAARRALSLWAETYGLCPAAAGILPDGYAEDEPCPVYVSGRCDKACG
                     RSDEQVLAFAHKLPVLDWGKMHEVEITETDPLTGEKVVLHGMGGALEMDDGFWYFDKD
                     LLDAFKAKFKTDRKNIKEIG"
     gene            11413..11489
                     /locus_tag="WGR04_000950"
     tRNA            11413..11489
                     /locus_tag="WGR04_000950"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11447..11449,aa:Arg,seq:acg)
     gene            11507..11581
                     /locus_tag="WGR04_000951"
     tRNA            11507..11581
                     /locus_tag="WGR04_000951"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11540..11542,aa:Glu,seq:ttc)
     gene            12140..13315
                     /locus_tag="WGR04_000952"
     CDS             12140..13315
                     /locus_tag="WGR04_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ0794525.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIRREGKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(13526..14074)
                     /locus_tag="WGR04_000953"
     CDS             complement(13526..14074)
                     /locus_tag="WGR04_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ0794526.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDVAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNTESK"
     gene            complement(14077..15573)
                     /gene="ccoG"
                     /locus_tag="WGR04_000954"
     CDS             complement(14077..15573)
                     /gene="ccoG"
                     /locus_tag="WGR04_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ0794527.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     TAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLAVVIAAFLVGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            16103..18082
                     /gene="tkt"
                     /locus_tag="WGR04_000955"
     CDS             16103..18082
                     /gene="tkt"
                     /locus_tag="WGR04_000955"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ0794528.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFILSNGHASMLLYSLLHLTGYNLGIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEKGAKLEAEWDKLFAQYQAKYPAEAAEFVRRMDKKLPENF
                     DEYVQTALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLALHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLDDIKRGGYIISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            18227..19615
                     /gene="fumC"
                     /locus_tag="WGR04_000956"
     CDS             18227..19615
                     /gene="fumC"
                     /locus_tag="WGR04_000956"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ0794529.1"
                     /translation="MSTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(19680..20603)
                     /locus_tag="WGR04_000957"
     CDS             complement(19680..20603)
                     /locus_tag="WGR04_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0794530.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALVVVGGAVTAAVGDRLFKRR"
BASE COUNT         5111 a         5883 c         5042 g         4735 t
ORIGIN      
        1 caaaactcaa atcaagccgt tcggaggcgg ctcaaaaaaa cggtacttcg cagcagaagt
       61 accgtttatc gggatttcag gttttattct tcggggcgtt cgccgtcggt ttcgtcctgc
      121 gtcccttcgg tgatgtgcat ttctacgccg ttgagggcgc ggatttttgc gtcgatttca
      181 ttggcgactt cgggattttc cttcaaccag acacggacgt tgtccttgcc ctgcccgatt
      241 ttcgcaccgt tgtagctgta ccacgcgccg gatttgttga cgatgtcgtt tttcacgccg
      301 atgtcgatca attcgccttc ccaactgatg ccttctccgt agaggatgtc aaactctgcc
      361 tgacggaacg ggggggcgac tttgtttttg atgactttga cgcgggtttc gttgcccaat
      421 acctcttcgc cttttttgat ggatccggtg cggcggatgt cgaggcggac ggaagaatag
      481 aatttcagcg cgttgccgcc ggtggtggtt tcggggctgc cgaacattac gccgatcttc
      541 atccggattt ggttgatgaa cacaaccagc gtgttggttt ttttgatgtg tccggtcagt
      601 ttacgcaaag cctggctcat caggcgcgcc tgcagtccga cgtggctgtc ccccatatcg
      661 ccttcgattt cggctttggg gacaagtgcg gctacggaat cgacgactac catatctacg
      721 ccgccggagc gcacgagcgt atcgcagatt tccaaagcct gttcgccggt atcgggctgg
      781 gacaggtaaa gctcttcgac ttttacgccg agtttgcggg cgtaaacggg atcgaaggcg
      841 tgttcggcat cgacaaaggc gcacacgccg ccgtttttct ggcattgggc gacggcttcg
      901 aggcagaggg tggttttgcc ggaggattct gggccgaaga tttcgacgat gcgcccgcgc
      961 ggcagaccgc cgactccgag ggcgaggtct aatccgagcg atccggtgga aatgacttcg
     1021 aggttttctt cctgctggct gccgtccatt ttcatgatgg cgcctttgcc gaaacttttt
     1081 tcgatttgcg ccagtgcggc ggcaagtgct ttgcttttgt cgtctgacat tggggttact
     1141 ccggaacaaa tgcggtatgt gggatgcggc gcaacacggg ctgcggcgcg ggatgtgtat
     1201 cgttttcccg atgtgcgggc tatcggtaat gctgcttcac gaggttgcca ttatcgcata
     1261 tttccttgct tgccgatatg cgccaggacg cggcggcttg tgccggaatg gaatctggat
     1321 gccgtctaaa aggcggccgg ctttgttata atggcggctg ttttttctgt gtgtgcctgt
     1381 tttatgtgtt cctgccttgt tgtcaaaaat accgttatcg gaagcggacg caccaaaatc
     1441 gccgtgccgc ttgtcgcccg cgatgccgcc gaactttccg ccgtacttga gcaaatcaaa
     1501 aatatgccct tcgatattgc ggagttccgc gccgactttt tggaatgcgc gggcagtatc
     1561 ggcgaaatat tgcaccacac gcagaccgtc cgcgacgcgc tgcccgacaa gccgctgctg
     1621 tttacgttca gacggcatgg cgaaggcggc tcgttcccgt gttcggacga ttattatttt
     1681 aaactgctcg acgcgctgat cgaaagtcgc ctgcccgaca ttatcgatat cgagctgttt
     1741 tccggcgaaa ccgccgtccg gcgcgctgtg gcgaatgctc aaaaaaacgg catcgccgcc
     1801 ctgctctgca atcatgagtt tcaccgcacg ccgccgcaag aagaaatcgt atgccgtctg
     1861 aaacagatgg aggactgcgg cgcggacatc tgcaaaattg cggtgatgcc gcaaagcgcg
     1921 gaagatgtgc tgactttgct ttccgccacg ctcaaagcga aagagcttgc cgccaaaccg
     1981 attgttacga tgtcgatggg gcagacgggg gcggtcagcc ggcttgccgg acaggtgttc
     2041 ggctcaagca tcacgttcgg ttcgggaacg caaaactccg cgccggggca aatcggcgta
     2101 tccgccctcc gtgcgacact cgactgcctc gaaaacggcg cagactgatt tcagacagca
     2161 tcaaaacatg atgaaactca atccccaaca gctcgaagcc gtccgctacc tcggcggccc
     2221 actgctcgtc cttgccggtg caggcagcgg caaaaccggc gtgattactc aaaaaattaa
     2281 gcatttgatt gtcaatgtcg gctacctgcc gcataccgtt gccgcaatta cctttaccaa
     2341 caaagccgct gcggaaatgc aggagcgcgt tgccaaaatg ctgcccaaac cgcaaacgcg
     2401 cgggctgacg atttgcacgt tccactcttt gggcatgaag attctgcgcg aagaggcgaa
     2461 ccatatcggt tacaaaaaaa acttctccat tctcgattct accgacagcg cgaaaatcat
     2521 cggcgaactc ttaggcggta cgggcaaaga agccgtattc aaggcgcagc accagatttc
     2581 cttgtggaaa aacgatttaa aaacgcctga agatgtcgtt cagacggcat cgaacatttg
     2641 ggaacaacaa accgcacgcg tgtatgcgag ctatcaggaa accttacaaa gctatcaggc
     2701 agtggacttc gacgacttga tccgcctgcc tgccgtgctg ttgcagcaaa acagcgaagt
     2761 gcgcaacaaa tggcagcggc ggctgcgtta tctgctggtt gacgaatgcc aagatacgaa
     2821 tacctgccaa tttacgttga tgaaacttct gaccggcgcg gaaggtatgt ttaccgccgt
     2881 cggcgacgac gaccagtcca tctacgcatg gcgcggcgcg aacatggaaa acctgcgcaa
     2941 aatgcaggaa aactatccgc agatgaaggt catcaaactg gagcaaaact accgctccac
     3001 cgcgcggatt ctcaaaatcg ccaacaaagt catcgagaac aaccccaagc tgttcaccaa
     3061 aaaactttgg tcgaaattgg gcgaaggcga gcccgtcaaa gtcgttgcct gccaaaacga
     3121 gcagcacgaa gccgactggg tcgtcagcca aatcgtcaaa caaaaactca tcggcggcga
     3181 caaaacccaa tatgccgatt tcgccgtgtt ataccgcgga aagcatcagg cgaggatttt
     3241 cgaggaagca ttgcgcggcg cgcgcatccc ctaccggctc tccggcggac aaagcttttt
     3301 cgacaaagcc gaaatcaaag acgtgttgtc ttatgtgcgg ctgcttgcca accccaacga
     3361 cgatcccgcc tttctgcgtg ccgttaccac gcccaaacgc ggcatcggcg atgtaacgct
     3421 gggcaagctc aacacttacg cgcacgaaca cgaatgcagc ctgtatgaag ccgcgcaaaa
     3481 cgaagaagcc ctcgccacgc tgaacaatac caaccgccaa cacctgcaag cctttatgga
     3541 tatgttcggc agttaccgtg ccaaagccga aaccggcgaa gcgggcgagt tcatcaacag
     3601 cctgctcgaa gaaatcgact atgaaaacca cctgcttgcc agtgaagaag gcaaagctgg
     3661 cgaaatcaaa tggcgcaacg tcggcgattt ggtatcatgg tttgcgcgaa aaggcgggga
     3721 agacggcaaa aacatcatcg aactcgccca aaccgtcgcc ttgatgacgc ttttggaagg
     3781 aaaagacgaa gaagaaaccg atgccgtctc gctatccacg ctacacgccg ccaaaggttt
     3841 ggagtatccg tatgttttcc ttgtcggttg cgaagaaggc gttttgccgc acaacgacag
     3901 catcgaagaa ggcaacgtcg aagaagaacg ccgcctgatg tacgtcggca tcacccgcgc
     3961 caaacgccaa ctcacactga cccactgcgt caaacgcaaa aaacaaggca catggcagtt
     4021 ccccgaaccc agccgattca tagacgaaat gccgcaggaa gatttgaaaa tcctgggtcg
     4081 caaaggcggc gagccgattg tcagcaaaga agaaggcaga cgcaaccttg ccgatataat
     4141 cggaaggctc aacaacctaa aaaaaagcgg cgcggcggat taaaccggag ccgcaatgcc
     4201 gtctgaaggc ttcagacggc atattttttg gacggcgcgc gtaaagcggt ttacgcccac
     4261 aaatcctgct gctggttttt cggcacaaga tgccccacgc cgataccgat aaggcggaac
     4321 gcgtcttccg tctgcggcgg gacgcgcgcc atcaacattt gcgcagcctg cagcagagct
     4381 gcgcagtcgg gcaatacgga ggaataagtc agtgtgcgcg tgatgatgcg gaaatcgtag
     4441 gtcttcagct tgagcgttac gctttgggct tcgacgtttt tgcgcgtgat ttgccgccac
     4501 aagtcttcgg caagatgggg gaggtgtccg gcagcctgct cgagcggcag gtcttcgggc
     4561 agggtaattt ctgtggagat ttggaggcgt tcgcgttcgg ctttgacggg gcgttcgtcc
     4621 gtaccgcgca ccaaatcata gaggcggtat ccgtagcgtc cgaaatggtt taagagttcg
     4681 ccgcgctcga aacggcgcaa gtcgcccgcc gtccgcatac ccagcgactg catttttttc
     4741 agcgttacct tgcccacgcc ggggattttg cccaaaggca gggtttccaa aaatgccatg
     4801 actttgtgcg gcggcaacac aaactgcccg ttcggcttgc gccagtccga cgcgattttc
     4861 gccagaaatt tgttcggcgc gatgcctgcg gatgcagtca aacctgtttc cgcaaaaatg
     4921 gcggcacgga tttctttggc aacttcactg gcgtaaggga tgtttttgaa attacgggta
     4981 acgtcaagat aggcttcgtc cagcgacaag ggttcgatta aatcggtata acgcctgaat
     5041 acggcgtgaa tctgcgcgga aacctgacgg tacaaatcga aatgcggcgg cacatacacc
     5101 gcttgcggac acagcctttt cgccgttgcc accgacatcg cggaatgcag cccgaactgc
     5161 cgtgcctcat acgatgcggc gcaaatcacc gaacgcgcgc cctcccacgc gacgaccacc
     5221 ggccgccctt tcaaatgcgg ctgttcgcgc agttctaccg atgcgtagaa cgcgtccatg
     5281 tcgatgtgga tgattttgcg tgaagacatc ggctctgctg aggataaaag ggatattcta
     5341 ctgccggcat cgggcaaatt ccaaatatag tggattaaat ttaaaccagt acggcgttgc
     5401 ctcgccttgc cgtactattt gtactgtctg cggcttcgtc gccttgtcct gatttttgtt
     5461 aatccactat atacgtcccg atagacctgc ctccataaaa atgccgtctg aaacataccc
     5521 tgtttcagac ggcatccgca aaactacagt tttcaattaa aactgccaat ccagtttcat
     5581 gctgacagtg cgcggctctc catagaagtt gtttgcgccg cgcgtacggt tgtagttgtt
     5641 ctcaaaataa gtgcgtccgt ttaagttcgt accgatgagg ctcaatttgg cgtgtttgcc
     5701 caattcgtaa cggacgaaac cgtctatcag cccgtagccg ccctgacgga tgttgtacag
     5761 gctgcttgtg ccgctttgtg cggacacgcc gccgccgacg gtcagccccg tattcggtat
     5821 atggaagctc gttccgaaac ggaatatgtg cacgggtgtg aaattgctga agttgtacgg
     5881 gtctgcgcct gtgtttttgg cgaggcgttc ggcgttgact tcggcggcgt ttttgtagcg
     5941 gctcttgttg taggtgtaac ccgcaaagac tttccaatct tcgttcaact cacccgacaa
     6001 ctcgaattcc gcacccctgc tgaccacttt gcctatcggt ttggcaacgg tttggaacga
     6061 cccctgcttg ccgcctgctc tgggaacata gccaaaatcg acgaccgtgc ggtttttctg
     6121 ttcgaggtaa aacaatgcga acgaagcatt cagccgtcct tgcaagaacg cgcctttcca
     6181 gcctacctca tagtttgtgc cgaccaaagg cggtaaaacg gttttggcac tgacatcgac
     6241 attatcctgc tgtttgaaga ttttggtata acttccgtaa atactctgtt gcggtgtcaa
     6301 atcataggta atgcctgcat agggcatcaa tttatgacct tgcatcttgg ccgtataatg
     6361 gtcctgatcc gccttaatgc tcgatgccgt ctgaaaatcg cttgccggct gcccatagcg
     6421 gacaggcata tctttggttt gcgaggtttc atagcgcgtg tagtgcagcc cgcccaaaag
     6481 gtgcagtcgg ccggttacgt tgaaacgcgt gctggcagtc agcgaatggg ttttgttggt
     6541 gttgaggtat ttggcgtagt tatacagcgc aggaacatgg tcatcggcca ctttaacagt
     6601 tttccaaacc ggcaccgtac cggaaaaacc ggtaaagaca ggcgagccgt cgggattggt
     6661 ctcctgaatc ttgttgcctt tttcgtccag ctcatataca tcgacatata ccggtgtccg
     6721 gctgccgctg tattcgtcat agtaatacac ctgcttgcct tcggcatcga gcttgggctc
     6781 ggtttttatt ttcttggcat tcctgcattc ttcggcataa acggtacggt tgcctttttc
     6841 atcgtacgcc tgccaatcgg gttctttatg ccccctgacc aaaggagaag acaaatcacc
     6901 gtccggctcc tcctgacaac ttcccgcata cacgccgtgc gttgcccctg tattcggacg
     6961 tactctgtgg cggcgttcgt agatttccag atattcggaa cgtatctttt catcgccgta
     7021 ggcatagccg acaaagaaat catgctcccg cccgaacagc ccatatgtgc cggtcaggtc
     7081 aagtttaatt ccccattggc ggtcgtcttt ggtatgccgc aacggcatat aactgtatcg
     7141 tcggttggcg gtagccttcc gattaaagga agagtcatac aggctgtttg gaaaacgttg
     7201 tgccgcatta ttaaagatgc cttccttcgc aagggcttta tcgacaaatt ccgacttgtc
     7261 ggcatcaaca cccagatccc cccaagaacc ttgacagata aagtccagcg caaaaggggc
     7321 actcatacac tcgtcaaaac cggctttgcg ttctgcggca cggcggctgc gatactgttc
     7381 gaaagcagta ttatcgaaac ggtttttaac aaaatcgtct ttgcgctcac ggtattcctt
     7441 ggcggtttcg tcacgatatg ctttcagttt ctccaatgcc ttatctttcg gctcgaacgg
     7501 gatgacttcg tttttttcgg tcaaaaagcc taccgcatcc tcatctgaca aacccgccgc
     7561 atgttcgttt ttcagaaaaa actgccccac cttcgcatcg gattcattct tggtataaga
     7621 cacttcggca ttgagctgcc aaccgttgtc aaacacatgt ttgaaacctg agaaaaggtt
     7681 gtatttgtca gcactcaacc gcgaccaatc ctcccccaaa taagtgttgc gcggcagttg
     7741 caaaggccgg ttgcaggcag gcgttgaact gaacggggca gttttctgat tttcacagga
     7801 caaaataatg cccgaaaaat caggaacctc cctactcttc tgatacatgc cgcccaaagt
     7861 aagcacactg ctgtcgcccg catcggcttc ggcaatgccg taaaccatat gtttcctgcc
     7921 ccaaacgcgg tctttaaacg attttttata ctcttccgca cccaccaacc ttccgcgtaa
     7981 ggtattcgcc ttattcaggc tgcctgaaac atccaacact gcacgccggc tgccgcgatg
     8041 gtcggcggtc agctctccgg tatgtttgaa agaagcggta ggtcgcttac ggatcaaatt
     8101 gacggttcca cccggctctg aattggattg ggtcaacccc gttgcacccc gtacaacttc
     8161 aatatggtca taaaccgcca aatcggtact cggagacacg tcgattttcg ccgtatatcc
     8221 cgaacggcct gcaacattga cggtaatacc gtcttcacca atctgatcaa tatagaaacc
     8281 gcgtgacaaa aaccgcgtct gcaagcctga atcgcgcaca acgttgacac ccgtcgtgtt
     8341 tttcattgcc tcttcaagcg tatgcaccgc cttatcgtca aggcggctgc gcgtgatgac
     8401 gctgaccgac tgcggcgtat ccttgcccgc aatcctcata cctgtggcgg tggacatccg
     8461 atctatcgta taagaacggg tcttctcggt cttgcccgac agagcatgag agccgcgtac
     8521 attgaccgta tccagactga cggtattgcc gtctgaaaca ggcacaacac cgtctgcaaa
     8581 agaaccaccg taagccgata acagcataac ggtcagaatt ttaagtgaaa aatgattttg
     8641 attcatagag acctctgtaa tatgcaagtg tgcaaatcgt ccaaaggccc tcacaactgt
     8701 tttgattttt tatattaatt gaaaaaaagt aattctcaat taaatttata gatgggattg
     8761 tattcccatt ttgacaaaaa acaaactgct ccttaccgtt tatttcaaaa aacgataata
     8821 ttgtattgaa aaatatccga atttaaatac agatcgtcaa tgcggaaaaa aacacccaaa
     8881 ttggctataa tcccgacaaa cacactcaag gacaacaaca tggcagcctc gcccgaagca
     8941 aaattcactg aagaaaagat tttgtgggtc aaacaccaca cgccgaaact catcactttc
     9001 gccatcagcc gtcccgaatc ctaccgtttt aaagccggac agttctcccg actcggtttc
     9061 tacgaagggg aaggctttat ttggcgtgcc tactccatcg tttccgcaga atatgcagac
     9121 acgctcgaat attttgccgt actcatccaa gacggcccca tgtcggcccg tttcgccaaa
     9181 atgcaacagg gcgacaccat cctgctcgat aaaaatgcca ccggcttcct cctgcccgaa
     9241 cgcttccccg acggcaagga tttggtgatg ctctgcaccg gctccggcat cgcccccttc
     9301 ctttccattc tcgaacaacc tgaaatccga cagcgtttcg ataccgtcaa cctgatacat
     9361 tccgtatctt ttcccgaaga attgattttc aacgaccggc tcgccgcatt gtccgaacat
     9421 cccctggtag gcgaatacgg acactcgttc catttcgtcc ctgttaccac ccgtgccgcc
     9481 aacccctcgg gcttaagcgg aaaacgcatt ccggaactct taaaaaacag cagcatcgaa
     9541 caggcgctgc ataccaagtt caccccggaa tccacacggt ttatgatttg cggcaacccg
     9601 gaaatggtca aagatacttt ccaaacgctg cttgacatgg gttacgccat gcaccgcaac
     9661 cgcattcccg gtcaaatcat gatggaaaac ggcttctaaa aaccaccctg cttgtccgat
     9721 gccttcggat ggacggtcaa accgacacgg cacgaaaacc gcgtcggcaa aaatgccgtc
     9781 tggaaaaatt cagacggcat cttcggacac attacctgca aacggcaaca cattccaacc
     9841 caaaccgatt aggcaatcaa tacgaacggc tgtttacata cttaccggct ttcatcaacc
     9901 ggtatcgatt taaccgattt ccttaatatt tttcctgtcc gttttaaact tcgccttaaa
     9961 cgcatccagt aaatctttat cgaaatacca aaacccgtca tccatttcca atgcgccgcc
    10021 cattccatgc agaacaactt tttcccccgt taaaggatcg gtttcggtaa tttccacctc
    10081 atgcattttt ccccaatcca aaacaggcaa cttgtgtgca aacgccaaaa cctgttcgtc
    10141 agaacggcca cacgccttat cgcatcggcc tgaaacatat acgggacaag gctcatcctc
    10201 tgcataaccg tccggaagga taccggctgc ggcaggacac aatccgtatg tttccgccca
    10261 caacgacaaa gcccgtctcg ctgccttttt attggcaaat aatccggtag gcggattatc
    10321 cgtcacaccg tttttcaaag ccgctgtttt cgcattcaac atgccgtctg aacctttttc
    10381 aaacctgacg gtcgtaaatg ttttaagcag atttttggca gatacataac aacccgaatg
    10441 ataacgtccg accaattccg ctttaatctt atatgcatgc aggctgccca atgcaggaaa
    10501 aaaacggact tcctccgtat tgcaccaatc aaacggggct tttccggaat ccaataaagc
    10561 cgaaacctcg ctatataccc gttcaaacgt accgatgtaa tttacttttc ctccgccgtc
    10621 gaaacaggcc agcaccccca taccgtcagg caaaccgtac aactgttccc tcaaccgttc
    10681 gggcagcgcg gcaggcagcg gtttcggatt catcaaacgg aaacactgcc tgatccatgc
    10741 ctcaaccccg tgttccgaca gactgtattc caaataatca cacaatgccg atacatccgc
    10801 catcgcacga tgcctgtctt ccacaacaat ccccaacctt tcgatgatac tgtccaggct
    10861 gtgcttgtaa aattgcggat acagacaccg ggacagctgc acactgcaca aagcaggcga
    10921 tgaaaatccg atacccgcac gatgaaactc atgctttaaa aacgtatagt cgaaacggct
    10981 gttatgtgca accagcacac aacccttcaa taccgaaaac aactcgccgg caatctctgc
    11041 aaaaacaggc gcatcggcaa ccatgccgtc tgaaatcccc gtcagccccg ccacaaactg
    11101 cggaatcggt ttttgaggat taaccagcca ctcatgcctc accacccttc cctgctcaaa
    11161 cttgaccaaa gccacttcgg ttaccctgtc ttcatacaga ttgccgcccg tcgattccaa
    11221 atcgaccacg gcaaccggca ttccaaaccg taaaaacgcc ttttccagca agggccaacg
    11281 ggaagcaaca atcattttat tctctttaaa ttcaaacaac aaaccgatat tttacacttt
    11341 taaggcattt catccaacaa aacaattgac agaatccgat gattacccta aaattcaaat
    11401 ctttcttgca gtgcacccgt agctcagttg gatagagtat ctggctacga accagagggt
    11461 cgggcgttcg aatcgctccg ggtgcgccag taagaaaata caatatgcgc ccatcgtcta
    11521 gcggttagga catcgccctt tcacggcggt aaccggggtt cgattccccg tgggcgtgcc
    11581 aaattccaaa tccccgagat tatccctcgg ggatttttta ttgcctcggc aactcgttac
    11641 catatcttta cctaccccct tcatcagaat ctcagacgta atcgaatcat attcaaacct
    11701 ttgccgtgca aaccgatatc ccataaccgg atgcggtgtc cgtccaacat tttacccgat
    11761 tgaaacgcct gatatattgc accccatcaa cgtggcatta cttttcttaa caatcccctt
    11821 tgacagcaac tgactagggc ttttttatgc catcatcaaa tttatagtgg attaacttta
    11881 aaccagtacg gcgttgcctc gccttgccgt actatttgta ctgtctgcgg cttcgtcgcc
    11941 ttgtcctgat ttttgttaat ccactataat attttctctc ccgattgaaa caggcgtaac
    12001 agaatatgcc cgaagctccg gctgctttct tgtttaccgc cgcgatattt agagtataat
    12061 accaaatttg agcaatggtt ctaaaacagt tagaaccatt tttcatgagc ctgactgatt
    12121 cgtacactcg gagaaactga tgcagaatat ttttgaccct ttggttattc gtggaaaatc
    12181 ccttaccccc atcgtgcaag gcggtatggg ggtcggtgtt tccgcatcgg gtttgtccag
    12241 cgcggtggca cgtgaaaacg gtatcggaac gattgccagt gtggatttgc gccaccttca
    12301 cgaagaccta ctcgccgaat cccaaatcaa tccgagtgaa gagaaatata cgtctttgaa
    12361 ctgtaccgca ttggacaggg aaatccaaaa agccaaaagt gcttcagagg gaaaagggct
    12421 gattgcggtc aacgtgatga aggcggtcaa agaccacgcc gcatatgtcc gccaggcttg
    12481 cgaatcaggg gcggatgcgg tcgtgatggg cgcaggcctg cctttggacc tgccggaaat
    12541 gaccgagggc tatcataaag atgtcgcgct gctgccgatt ctgtccgaat cgcgcggtat
    12601 taatatcgtc ttgaaacgtt ggatgaaaaa aggcatattg cccgatgcga ttgtagtcga
    12661 acatcctgcc cacgcggccg ggcatttggg cgcgtcaacc gtcgaaggcg taaacgatgc
    12721 taaattcgac ttcaaacgcg tgattgagga aacgtttgaa gttttcaaaa gtttagggct
    12781 ggaaagcgaa aaaatcccgc ttattttggc aggcggtatg gcaaattttg aaaaagtcaa
    12841 aaccgcccta aagaactggg gagcatccgc cgttcaaatc ggtacggctt ttgccgttac
    12901 cgaagaagga gatgcacacc ttaacttcaa aaaaacgctc gccggtgcgg aaactgaaaa
    12961 agtagttgaa tttatgtctg ttgccggttt gccggcgcgc ggcgtacgta ccaaattcct
    13021 agacagctat atcaggcgcg aaggcaaact tcaggcaaac gccaaagccg acccgcgccg
    13081 ctgcacccaa ggattaaact gcctgaccag ctgcggcctg cgcgacgggc tttccaaagc
    13141 cgggcaattc tgtattgata tccagcttgc tgccgcattc cgtggagaag tggataaagg
    13201 cctgttcttc agaggtaaag acccgctgcc cttcggcaat gccatccgca ccgtccgcga
    13261 gacgatacaa tatctgctga cggggagcga acctgttgca acgctcggac gctgacatca
    13321 ggtaggattt gatttgcaaa caaaatgccg tctgaagggc cttcagacgg cgtttttcag
    13381 gctgcgttct ggaatagtgt taaaaaataa acgggatgag ctgcatttat tttcgtccga
    13441 caaatcaaac catcacgccg aatgatcaaa taatgcctgc acggcgttgc atctggcaaa
    13501 tcaatgcaat gaaaacacgg cttttttatt tgctttcagt attattgaaa agtttgtcca
    13561 tcggggtcaa atcgaccgca ttgccttggc tggtaatcca tttgttctcg acgcgccaca
    13621 cgcctgccac gtcctccacg cgcacatacc agtggttggt cggcgaaagg gttttgaaca
    13681 ccgcctcata ttccgccctg ccgttctgcg cgctgccgac gggcttgagg gcgacagttt
    13741 ggtcgtccgc cttgcgggtc gggtgcatca gcagcaggtt caaaggctgt ttgccgtcaa
    13801 actcgccgcc gacaaacact tttgccgcat tcatatcggg ggaaatgaga acctgcaccc
    13861 cgatatgccg tctgacggct tcttcatccc gatgaagctg gatgtcgata tgtttgccgt
    13921 ctttataata atcgtccgta accaaatctg tcgcgtgctg ctgcgcgaca aaaaacatag
    13981 cgacgctggc gatgacgaca aaaatcggcc ccgccatcaa gatccacggc cagacgtgtt
    14041 tgtaccaagg tttgattgga gtgttttgag acacggttat tctccgataa aggttgcatc
    14101 ttcttccaag acgaccggct tgccgtcggg cgcgccgctt tcgcggtatt ggaaggtaaa
    14161 ttcgataggg tggctgcctt tgtccgcgta ttccggaatg gtggacactt ggacgggaag
    14221 ggttaccgtt tcgcgcgggg caaccttgat accgccttcg ggcagcccgg tcagggcgat
    14281 ttcgtcaaag cctttgacac ttgcggtaat gagctgttct ttttcacttt tgttgatgat
    14341 acgcaggctg tatgcgtttt ccagccagcc tttggcgttt tcgcgcacca gtacgccacg
    14401 gtctttcaaa atatcgacct cgaccatttt gcgcgtggac aaaccgacca aaaaggcggc
    14461 gataaccact gccaacaccg cgccgtaacc tgccacgcgc ggtctgagca gccgtttttt
    14521 aatgtctttt tcagaatatt cgtgttccaa cgcgctttcg gtcgtataac ggattaatcc
    14581 gcgcggatag cccattttgt ccataatctc atcacacgcg tcgatacagg cggcgcagcc
    14641 gatacattgg tactgcagac cgttgcggat gtcgatgccg acagggcaga cttggacgca
    14701 catcgcacag ttgatgcagt cgcccaaacc cgcctcttcc ttattgaccg ttttcttgcg
    14761 cgcgccgcgc ggttcgccgc gttccgcgtc ataagaaaca atcagcgtgt ccttgtcgaa
    14821 catcgcgctt tggaaacgtg catacggaca catatgcagg catacttttt cgcgcataat
    14881 gtgggcgaag aagaaggtca taaagccgta aaacgctgtg gcaaacatcg cgccgccgcc
    14941 tgctgctcca gtgaataaat cgggaacgaa ctggcggata gggacaaacc agcctgcaaa
    15001 cgtgatgccc gtccacgcgc agacaaggaa aatcaacagg tatttggtgg ctttgatgcg
    15061 gattttagtg aaattccacg gcgatttttc cagtttcagc cgtttgtttc tatcgccttc
    15121 gaccaggttg tcgatccaca gcataatttc ggtgtaaacc gtttgcgggc aggaatagcc
    15181 gcaccacagt cgcccggcaa tcgtcgtcca ccaaaacagc ccgaaggcgc aaatcatcag
    15241 cagcaaggca aggtaaatca aatcgcccac ccccaacgac aatccgaaaa tgaagaaatg
    15301 ccgttcgggg atattgaaaa cgacagcctg cctgccgctc cagttgaacc acggaatgac
    15361 gtaaaacaca aactgcgtcg ccaatacggc ggcgatacgc agtttggcga accgtccttc
    15421 cgcctttttc ggatggatgc gttcgccttc gggatggatt tgaatcacgc tggctcgcgg
    15481 atcgaacgtt ttttttgctt tcggcgcggc ttttgtttgt tcggacgtgc cgattccgga
    15541 tgccggactg ccggcttggt tttccgtggt cattctgcat tccttagatt tttgattgat
    15601 ggtttgccct ttaccgccgc cgtttgcttt tcagacgtca tttttcttgt tttttaaggc
    15661 gttgtgtttc aagttttgag aaaatccgtt tttcccaaaa tatatttccg ctattgtaca
    15721 actttatgcg ccgtccggat gtatggggcg gatacatttc ccatccgcat caaaacgcct
    15781 ggattttacc ttaccgcccg aacaaaatcc gaatacggtt aaaaaaaaag actaaaaaaa
    15841 aaccgacacc cccatatcgg cagaaccgac ggcgcaagct cataaacaaa cgctatcgac
    15901 aatccggcac acaatctata actttttatt tcaaaaggaa taatggcagg cttcgcccgc
    15961 aaatcgaaaa tccttcccca ccggtctcct gccgccgcct tcccccgcgt ccgccctttt
    16021 cttgaaagca taagcgaatc gggcgataat caacgccttc cgattatcca ctcatccgaa
    16081 acaccagcaa ggaaaataca aaatgtctca actggcaaac gcaatccgct tcctctcggc
    16141 agatgccgtt caaaaagcca attccggcca ccccggcgcg cctatgggta tggcggaaat
    16201 ggcggaaaca ttgtggacga aattcctcaa tcacaacccc gccaacccca aattctataa
    16261 ccgcgaccgc ttcatcctct ccaacggcca cgcgtctatg ctgttgtaca gcctgctgca
    16321 cctgaccggc tacaatctgg gcattgaaga cctgaaaaac ttccgccagc tgcacagcaa
    16381 aacccccggc caccccgaat acggctacac cgacggcgtg gaaaccacga ccggcccgtt
    16441 ggggcaaggg attgccaacg cggtgggtat ggcattggca gaaaaaatcc ttgccgccga
    16501 atttaataaa gacggtttga acatcgtcga tcattacacc tacgtcttta tgggcgacgg
    16561 ctgtctgatg gaaggcgtat cgcacgaagc ctgttcgctc gccggcacct tgggcttggg
    16621 caaactgatt gttttgtatg atgacaacaa tatttccatt gatggtaaag tggacggctg
    16681 gtttaccgaa aacatcccgc aacgctttga aagctacggc tggcacgtcg ttcctaacgt
    16741 aaacggtcat gacaccgccg ccattcaagc cgccatcgaa gccgcacgtg ccgaaaccgg
    16801 caaaccgtcc atcatctgct gtaaaacctt aatcggcaaa ggcagtgcca ataaagaagg
    16861 cagccacaaa acccacggcg cacctttggg cgcggacgaa atcgaagcca cgcgcaaaca
    16921 tttgggctgg acttaccctg cctttgaaat cccgcaagaa atttacgatg cgtggaatgc
    16981 caaagaaaaa ggcgcgaaac tggaagccga atgggacaaa ctgttcgcgc aatatcaagc
    17041 caaatatcct gccgaagccg cagaatttgt gcgccgtatg gataaaaaac tgccggaaaa
    17101 ctttgacgag tacgttcaga cggcattgaa agaagtgtgc gccaaagccg aaaccatcgc
    17161 tacccgcaaa gccagccaaa acagcatcga aatcttggca aaagagctgc ccgaactggt
    17221 gggcggttct gccgacctga ccccgtccaa cctgaccgac tggtcaaaca gcgtttccgt
    17281 tacccgcgac aaaggcggca actacatcca ctacggcgtg cgcgagttcg gtatgggcgc
    17341 gattatgaac ggtttggcat tgcacggcgg cgtaaaaccc ttcggcgcga ctttcctgat
    17401 gttcagcgaa tacgagcgca acgccctgcg tatggcggca ctgatgaaaa tcaaccctgt
    17461 atttgtgttc acccacgatt ccatcggttt gggcgaagac ggcccgaccc accagccgat
    17521 tgagcaaacc gccaccctgc gcctgattcc gaatatggac gtatggcggc cgtgcgacac
    17581 cgccgaatcc ttggtcgctt gggcagaagc cgtcaaagcc gccgatcacc cgtcctgcct
    17641 gattttcagc cgtcaaaacc tgaaattcca agcgcgcagc gaacaacaac tggacgacat
    17701 caaacgcggc ggctacatca tcagcgaagc ccaaggcaac gcccaagccg tcatcattgc
    17761 caccggctca gaagtcgagc tggctttgga agcgcaaaaa gccctcgccg cgcaaaacat
    17821 cgccgtgcgc gtcgtttcca tgccgtccac caacgtattc gaccgccaag acgccgccta
    17881 tcaagccgcc gtcctgcccg aaagcctgcc gcgcatcgcc gtagaagccg gacacgccga
    17941 cggctggtac aaatacgtcg gtctgaacgg cgcagtcgtc ggcatcaacc gcttcggcga
    18001 atccgcccct gccgatttac tcttcaaagc attcggcttt accgtggaca atgtggtcga
    18061 tacagtgaaa tccgtgctgt aaaccgcagc cgccgcatca aatgccgtct gaacgctttt
    18121 cagacggcat tttttttgcc ctccagcctt aatgcttcct cattccgcca atctccatta
    18181 taatatttgc gaatcactct tattcacatt tcaaaaggag aaacgcatga gcacccgtac
    18241 cgaacacgac accatgggca atgtcgaagt cccgtccgaa gcctattggg gcgcgcagac
    18301 ccagcgcagc cgcaacaatt tcaaaatcgg cggcgaaacc ctgccgcagc cgctgattta
    18361 tgctttggcg ctggtgaaaa aagccgccgc cgcgaccaat gtttccctcg gcaggattaa
    18421 gccggaacag gcggatttga ttacgcaggc ggcggatgat gtgttgaacg gcaggcttga
    18481 cggacagttt cctttagtgg tttggcagac cggctccggc acgcagtcca atatgaacat
    18541 gaacgaagtg ctggcaaacc gcgccaacga aatcgccggt acgggtttgg cggcttatca
    18601 gcccgtccat cccaacgacc atgtgaacca cgcgcaatcg accaacgacg cattcccgac
    18661 cgctatccac gttgccgccg cgattgaaat caaccgccac ctcatccccg ccgtaaaagc
    18721 cctgcgcgat accttggaca aaaaagccca agctttcgcc cccattgtta aaatcggccg
    18781 cacccacttg caagacgcga cgccgctgac tttgggacag gaattttccg gctacgtttc
    18841 ccagcttgat cacggcttag gccgtctgaa cgatgcgctt aaagacttgt atgaacttgc
    18901 tttgggcggt acggcggtcg gcacgggttt gaacagccat cccgaatacg ccgaaaaagc
    18961 cgccgccaaa ctcgccgaac tgtccggctt gccgtttgtc agcgcgccga acaaatttga
    19021 agccttaggc ggacgcgatg ccgccgttgc cgcttcgggc gcattgaaaa cgctggcggc
    19081 aagcctgaac aaaatcgcca acgacatccg ctggctggca agcggcccgc gttgcggttt
    19141 gggcgaaatc aaaatccccg aaaacgagcc gggttcgtcc atcatgccgg gcaaagtcaa
    19201 cccgacccaa tgcgaagcaa tgacgatggt gtgctgccaa gtgttcggca acgacgttac
    19261 catcggtatg gcgggcgcgt cgggcaattt cgagctgaac gtctatatgc ccgttatcgc
    19321 ctacaacctc ttgcaatcca tccgcctgtt gggcgacgcg tgcaacagct tcaacgaaca
    19381 ctgcgccgtc ggcatcgaac ccgtaccgga aaaaatcgac tatttcctgc accattccct
    19441 gatgctcgtt accgcgttga accgcaaaat cggttacgaa aacgccgcca aagtcgccaa
    19501 aaccgcctac aaaaacaaca aatcgttgcg cgaaaccgcc gttgagttgg gcttgctgac
    19561 gggcgaagaa tttgacgaac tggtcgttcc tgccgatatg gttcatccgc gctaatcttt
    19621 ccctcaaata aaatgccgtc tgaaacctcg ttcggacggc attttccgtt gcctgcaaac
    19681 tagcggcgtt tgaacagcct gtcccccacc gccgctgtaa ccgcaccccc gaccacgacc
    19741 agtgcgccgg cataacccaa accgttcata tccggcgcgg caaaagtatc aggcatcaca
    19801 taatgcccga gcaaagaaaa tattacggta aacacgggga gcaaggttgt taccgcgctg
    19861 actttggaag cctcccaatg tttcaacgcc tcgccgaacg agccgtaacc gattaacgta
    19921 ttcaagcagc aatacgcaaa acaaacccac gccaacgtac cgtccaaact tccgatgtgt
    19981 gccggttcgg caaacggcag gaacacggcg gcacttgccg cataaatcaa cagcagaatc
    20041 tgttgcggcc cgaattgcgc cgacagcagc ttttgcgcca cggcataaca cacccatgcc
    20101 atactgcctg ccgcacacag caacacgccc ttcgcatacg cgcccaaacc cgacaactcg
    20161 ccgaatttat cgttaaaaaa cataagcaaa ccggcaagca gcaaaaccaa gccgattttc
    20221 tgagcggcag tcatccggtc tttaaacacc aacacaccga caacaatcat cgtaaacggc
    20281 gaaatctgcc acaaaacctg cgtcgtggtc ggcgaaatat aatgcagccc ttgggcaatc
    20341 aacacaaagt ttgccgaaat gcccgccacg ccgagcagca gcagcctgaa tgagcaccaa
    20401 gaaaaatccc gccgcttcgg cagccgcccg cccaatgcca gcaaaacaaa caataccgcc
    20461 gccgccacgg taaaacgcac ccacaccagc gtcggcgcat cgacaaactt caatacctgc
    20521 cgcacggcaa tcggcagcgt tccccacgtc atcgccgcca aaagtgccaa cgcgaagcct
    20581 aggagcggcc tttggttttc catcctgatt ttcctatttt ttaaacaacc gtattgccgg
    20641 acgatgccgg tttgccgcat cgggcaatga tggttcaagc gtttggcgtt tgattccaac
    20701 cctttgattt caaacaaacc ggctgaagcc cggctattgc ttcgcgctat ttgaaaacac
    20761 cgcctgaatt t
//
LOCUS       DAWFJR010000028        20028 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWFJR010000028 DAWFJR010000000
VERSION     DAWFJR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20028
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.028"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(439..514)
                     /locus_tag="WGR04_000958"
     tRNA            complement(439..514)
                     /locus_tag="WGR04_000958"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(479..481),aa:Asn,seq:gtt)
     gene            complement(607..966)
                     /locus_tag="WGR04_000959"
     CDS             complement(607..966)
                     /locus_tag="WGR04_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ0794531.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(989..1633)
                     /locus_tag="WGR04_000960"
     CDS             complement(989..1633)
                     /locus_tag="WGR04_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ0794532.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1635..2171)
                     /locus_tag="WGR04_000961"
     CDS             complement(1635..2171)
                     /locus_tag="WGR04_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ0794533.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPSIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(2183..3280)
                     /locus_tag="WGR04_000962"
     CDS             complement(2183..3280)
                     /locus_tag="WGR04_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ0794534.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3283..4719)
                     /locus_tag="WGR04_000963"
     CDS             complement(3283..4719)
                     /locus_tag="WGR04_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ0794535.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4939..5721)
                     /gene="bioC"
                     /locus_tag="WGR04_000964"
     CDS             complement(4939..5721)
                     /gene="bioC"
                     /locus_tag="WGR04_000964"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ0794536.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQAWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFGMPSGKVRLTYHPLFFIARYSAAGRQ"
     gene            complement(5709..6356)
                     /locus_tag="WGR04_000965"
     CDS             complement(5709..6356)
                     /locus_tag="WGR04_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ0794537.1"
                     /translation="MKTKFYNHQGGHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKVSFEDYQQFPARPFDEIHQELTALFEMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6366..7508)
                     /gene="bioF"
                     /locus_tag="WGR04_000966"
     CDS             complement(6366..7508)
                     /gene="bioF"
                     /locus_tag="WGR04_000966"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ0794538.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEAALAK
                     AEYLQRQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAVCL"
     gene            complement(7530..7667)
                     /locus_tag="WGR04_000967"
     CDS             complement(7530..7667)
                     /locus_tag="WGR04_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HEZ0794539.1"
                     /translation="MATETNVAEISALDYEGRGVAKVGGKTVFINRALLDCLMLGWFR
                     L"
     gene            7731..8807
                     /locus_tag="WGR04_000968"
     CDS             7731..8807
                     /locus_tag="WGR04_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ0794540.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCIRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(9014..10480)
                     /locus_tag="WGR04_000969"
     CDS             complement(9014..10480)
                     /locus_tag="WGR04_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ0794541.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFLAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10678..11601)
                     /gene="speB"
                     /locus_tag="WGR04_000970"
     CDS             complement(10678..11601)
                     /gene="speB"
                     /locus_tag="WGR04_000970"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ0794542.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGIIDPAHSVQIGIRTEHSKKLPFTVLTAPQVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11700..13592)
                     /gene="speA"
                     /locus_tag="WGR04_000971"
     CDS             complement(11700..13592)
                     /gene="speA"
                     /locus_tag="WGR04_000971"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ0794543.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQIVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13759..14043)
                     /locus_tag="WGR04_000972"
     CDS             complement(13759..14043)
                     /locus_tag="WGR04_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0794544.1"
                     /translation="MKKLLLAAVVSLSAAAAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(14194..16002)
                     /gene="aspS"
                     /locus_tag="WGR04_000973"
     CDS             complement(14194..16002)
                     /gene="aspS"
                     /locus_tag="WGR04_000973"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ0794545.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFQDALGVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIARTGAQ
                     NGDIIFFGADKAKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKVAETKEA"
     gene            complement(16060..16761)
                     /locus_tag="WGR04_000974"
     CDS             complement(16060..16761)
                     /locus_tag="WGR04_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ0794546.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16904..18052)
                     /locus_tag="WGR04_000975"
     CDS             complement(16904..18052)
                     /locus_tag="WGR04_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ0794547.1"
                     /translation="MPTMGVEMNIRNMRYILLTGLLPTASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGRESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(18212..18475)
                     /gene="rpsT"
                     /locus_tag="WGR04_000976"
     CDS             complement(18212..18475)
                     /gene="rpsT"
                     /locus_tag="WGR04_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ0794548.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18779..19921
                     /locus_tag="WGR04_000977"
     CDS             18779..19921
                     /locus_tag="WGR04_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0794549.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANTEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5181 a         5158 c         4651 g         5038 t
ORIGIN      
        1 ttaagggggt atttgggtaa aattaggagg tgttatggag cgaaaacagc cgaaaacctg
       61 tgtttgggtt tcggctgtcg ggagggaaag gaattttgca aaggtctcaa ttagtatagt
      121 ggattaacaa aaatcaggac aagacgacga agccgcagac agtacaaata gtacgaaacc
      181 gattcacttg gtgcttcagc accttagaga atcgttctct ttgagctaag gcgagccaat
      241 gccgtactgg tttttgttaa tccactataa aatcgagttt atcctagttg tccaggacag
      301 cccctatttt ttcataacca tcacgaaagg aattttgcaa agattttatt ccatctcaaa
      361 acaatcatct caaaaatgcg tttctgaccg ccggtaaaac aaaaccctct aagaaaatac
      421 ttagagggtt ttgaaatttg gctccccgac ctgggctcga accagggacc tgcggattaa
      481 cagtccgtcg ctctaccgac tgagctatcg gggaataagc gcgaataata aaacagaaat
      541 accgaacgtg tcaatataaa ttgacaatcg attgcacttt atctgcaagc ccgcaatctt
      601 tatatgttat atggcttccg caatatgcct tgccgcacgt ttggcatcca ataggattaa
      661 accaagtctg ccggtttctt ttgccaccag caccaacacg gcatctttac ccgcctgact
      721 taaaaggata tagcctgatt ttcctttaat catcacttgt tccaattccc cgcaggcgag
      781 ttcctgcacc gagcgactcc ccaaagcaag caaagtggca gaaatcgccc ctaccctgtc
      841 cgaattcaaa tgtgaaggaa gcattgtcgc catcggcaat ccgtcggttg agataacggc
      901 agacgcgata atatccgtag atgtattgtt taaatcttca aggattgaaa tcaataattg
      961 ctgcataacc cctcctctct cccaagtttt acacgcggtt gctgtaacgg cggtataaaa
     1021 tccttaccaa agtaacaaat gcctctttgc ccaaatcggg aatgccgccg ataaccaaaa
     1081 taaatttggt tgaaccgata tacaatggga aaaatgtcaa ttcgctctga ccggaaggat
     1141 cgcaaacgcc ccaagcgtta ttgttgatat acaggttgtt cttaatcagc agccggtatt
     1201 tcttttccat ctgtgcgact tctgccgcca acaaccccaa ctcttccgcc gcctcatgat
     1261 ggaaattggc gttggcaaga tacagaccgt tccgatcgac caataacgcc ttaccgctgc
     1321 cggacaattg ttccatcagc aacggcaatt gctcgtccga caaattgatg ccgtctgaat
     1381 gaccgttttc atcgccatag aggaattcga gtttttgcaa acggtacaac aggttcaaag
     1441 cggtatcgat gtcggcggtg tccgcccagg taagcagctt ctcactgctg accatttcgt
     1501 ccgcatccgc tttcaacagg ctgtgcaaca aagttttacc ggcactgggg gcatcgctgg
     1561 atacggcata aaatgcaccg gcaggagtca ggcggggata taaatttgct tgtagtgaaa
     1621 gtgttgattc catattaaac ctccagtccc ggatcaatag aaaataacat tgcgctaacc
     1681 aattgtttta cgtcatcttc cttacgggca tcaatttcaa aaaccggaac attaagatta
     1741 tgttttgcaa gatatttgtg atacacgtcg atactgggct gagagcgtat atccatcttg
     1801 gtaataccga caacgacggg ggccttctcc agcagccctc gaaacgaatg taaaaagaat
     1861 tccaaatctt tcaacggatt ggttcgggca ttatctaaaa gcaagaccaa acccatactg
     1921 ccttggctta agatttccca cataaagttg aaccgttcct gaccgggcgt accatataaa
     1981 tggactttgg tatcctcatc caagctgatg gccccgtagt ccatcgccac tgtcgtattc
     2041 cttttcctat ccaaagtcat atcggatgcg gaagcatcgg tctgaacgag tgcttcgtcc
     2101 gaaatagccg caatggcagt ggttttccct acgccgacag gtcctgtgaa aataatttta
     2161 ttttctctca tctctccgcc tcttagctgc ccaacagttt tttcatcagc ctttgcagaa
     2221 ggccgcgcga ctgggattgg gatggtgttg tgattttttc cgcttttttc atcatttcac
     2281 tatcagaggc agaatcggct ccgatattta ttttatccgc catatcggag tatgcctgtt
     2341 gtgaaaccgt ttttaaatct accgacaaaa acccggttgt ataggttgcc gcaagataat
     2401 tcagaatatc attgaggttt aaaggcatca ctttatacaa cacgttaagg ttgacggatg
     2461 ccttggtcag aaatgccgac aagcgtatcg accccggcac atttgccaac cgggtcaggt
     2521 ttggccaaga tttcaacgta aacggagtat cgggagaaat cggataaatc aacctgccct
     2581 gcgccgtcca aatggaaaac tgccacatac aggacataat gcctacttta gccttttcgc
     2641 gccattgcgg gttatcggga acagtcttgc agctgacctg caaattttcg tctttgcaca
     2701 attcttcgag tttttgcaca ctttctgtca gcaaaacccg ttgtatcgag gggaaaacaa
     2761 taaggaccgg cttatttcca tgcaagatag cgatgtcctg cctgttcttt tccgcaaacc
     2821 gcaacgcccc caataatcct ttattcgggt taaactggcg tatcgtaacc gtacgctgca
     2881 cattcccgtt atttttcgcc gaccggtctg cgggtgcaat aaacgatttc ccataaacat
     2941 tctcgccctg caacaatttg cggagcatag gaaacaatgt ttcaaaccga atcggtttgg
     3001 gcaggtaggg aacttcagaa tcgggaactt tctccgaaca gacggcgacg ggtatatcct
     3061 tataacgctc ggcaagctct ttccaaagtt caaaaccgcc ctcggcatcg gtatccgcca
     3121 aaaccaaatc gggcacggca ctgccgtctg aaggggatac tgtttcataa cgggtggtat
     3181 tgtgcatttt gaatgccatt ttgaaaacgg attcctgctg cgccgtcatc cccgccaaca
     3241 ttacgcgtac tgttttaatt ttcggcagtt gaacttccat ttttattttc cgtaccgttt
     3301 tattttttta atattttgat tcatacgctg cagcagccgg ctcatcagca taacgacctc
     3361 ttcaggaagc ctgtccgcac gttccctcaa tacccttaaa aactgcccca acctatccca
     3421 atcttcagta cgttcataaa tatcgatcaa cgtaatataa agctgggact cgtcgggata
     3481 tttcaatacc gcctgctcca acacatccat tgccgcttca atctgaccat acatcagcaa
     3541 cgactctact tccttaaccg catcgtctgc cggagacgaa ccggtgttaa tcaacgaaga
     3601 atcttgaagc accaaatccc gatgttgcga tttgaatttc tgtatatttt tcggcagata
     3661 cccgtgcccc ataccgatgt ctttgatttg ccggtcgttc ggcccttttt ccaaatcatc
     3721 gaaaacttca tggtaaccca agctgtaccc ccaccccagc atccgctctt taacctgcct
     3781 gccgtagttg cccaacgttt ggtaaagttt ccacaaatgt ccggcaaaac ggtctatgtc
     3841 cgcgtgttgg taatcgagtt tcaacgcatc gataatcagg tttgcaggtt tttcggaagt
     3901 ttggatggca cggtcgtatt gtttcgatgc cgtttcatag ctgactttgt ctttaaggat
     3961 tttcgcacct tgatcggcgc gaaccaaacc ggcaatcgca ccgatttcct cttgactgat
     4021 tccggacacg tcttttttgc cccgcacaat cgggatgcgc ttgatttctt cggcttcata
     4081 agccttaccg ccggcatccg gcgggggcga tgcggaagct gccgtcgcag aaccgccttc
     4141 cgcacgtttt tcaatctcct gagtacccca tcccaaacct tcttctgcca agacgcggat
     4201 acgcaaatgg tttgaatcgc gctgtaatgc ctgttcgata tattttgcca aaagttcgga
     4261 aggaatcagt ttgccgtatt tttgcagatt gtctgccaaa acatcgacat cccccacttc
     4321 gagattgata tcgagcagct cgcggataag gttttcaggt ttcgcttcac catccggaat
     4381 gccgtccaga taggcagcca aagattcggc agccttgccc tgataaccga attgcttata
     4441 aacctgatac tccgtaagcg gatcgacttc ttgcgcggat acggcggcgg atgcggtctc
     4501 ggcactttcg ttccaagacc agtcgggttg cgtgccgtct gaaaccgatt gtccaatctg
     4561 atcgacccag tccgaatcat tgcttccggg tgtctgcccc tgcgtattgc cgacaccttg
     4621 ggatttgcgc ttcggtgttt tcttaccctg cttcgcacgc ataaccaaaa gcatcagcaa
     4681 taccgccagt gccaaaccga taattaatga gttttccaaa ggatactccc gataccgtaa
     4741 cgagcggata actgaccgcc tgcaacgacc attgtccgct tttattatta acgatttact
     4801 gaaatagact tgtaagtttt aaatcatacc ataatttaac gtttaacaat atgccttctg
     4861 cacaagcctc gccatattac cttttaccca catcagtatc aatacccgat ataaaaataa
     4921 ctttgcccat aagctgcctt attgcctgcc cgccgcagag tagcgcgcga taaaaaataa
     4981 cggatgataa gtcaggcgca ctttgcccga cggcataccg aacgccgaca agtaatcgcc
     5041 aataaatcca ttgagatttt ttcttgtcca atttttttgg ttcgtggctg ttacgcctgt
     5101 atatttaagg tgtttgagta catctaacgg cgtatcaaag tctagtatta ccttaaaatc
     5161 ctcacaccat aaaagctcaa aatctttggc taaccaagcc tgccattggg ataaagtcgg
     5221 gtaatttaag cctatatttg taatttggcg gacttctttt aaattgtctt tgccaaaggt
     5281 tgcaaccgcc aataatccgt ttgctttcaa gcctgttttg caatgggcga taaaagcgtc
     5341 gggttgatga aaccattgca cggcagatgc gcttgcgatt aagtcaaatt gtcgttgaaa
     5401 aggaaagttt tccgcatcgc cgcaataaaa atcaaaggat tgcggcagtt tttcagccag
     5461 ttggggctgc acatcgcaca aatcattaaa taaccaataa ttcgctgaaa tctgtttttg
     5521 cagcaaggca ctcaacattc ctgagccgca gcccaattcc aacacgtttt ccaatggcat
     5581 atccggcaaa taatcttgca aatgcgccat taaattaatc gtcatttttt gttggattaa
     5641 ggcgtgccgg tcataatcgt ttaatgcttt ttggaaagcc tgccgaatgc gcgatttatt
     5701 tatggcagtc agtgattcca tagtgccgac caatgggtga atcttgaaaa caggtaatgt
     5761 ccgccgtcaa tttcccgaac ggtgcaacgc ggtgtccaat atcggtgctg attggcaggc
     5821 ataaaaattt tatcgcctga tccgaccaag gcatttgtcc agcgaatcaa atctgcgcgc
     5881 ctatcttgcc cgatcatctc aaaaagtgcg gtaagttctt gatgaatttc gtcaaacggg
     5941 cgtgcgggaa attgttggta atcttcaaaa gatactttat cgccacacat tctgcgttca
     6001 aattttaaac gggtgttttc cgtgaggttc tccaatgtgc ctttaaaaac ggcgcaaggg
     6061 ataccgaaat tatcatcgca aggcaaacct gtgccattca ctgccgttgc ggattttaat
     6121 cttattcctt gcaatgccct ctctgccgcc caaacgccca ttgaccacgc caccaaacgg
     6181 atgtgccgat aggcggaaaa atcaaaatcc aaatttaaat cttgataatc gtagcaaatc
     6241 aataaatcgt gattttccgg caaaatcaaa tgatttacag catcgggcgg cgttccccaa
     6301 cctgcaaaat acaggattaa atgtccgcct tgatgattgt aaaattttgt tttcatatca
     6361 tatccttaca ggcacaccgc aaactgccgc acttcatccg ttgtcatatc tgccgttaaa
     6421 gacaggcgga ttctggatgt gtttttaggt actgtcggcg gtctgatggg caggcaataa
     6481 taaccctgcc tttgcagata ttccgctttg gcaagggcgg cttcattccc gcctaaaata
     6541 taggggacga tacaggtttg gctcggcatt atttgcgtcc gatgcgccac ttcccgccgt
     6601 aaaaatgcac ttaactgctc aagatggctt ctttcttttg agaattgcgg caatcgttca
     6661 aaaataaaat aagtccaagc cacattaaac ggcggcaatg cggttgaaaa aatcaatggg
     6721 cgcatttgat taatcaaaca ttctttcaat acttggttgc agacggcata cgcccccacc
     6781 gaggctaagg ctttaccgaa agtgccgacc aataaatcaa tctcggcaat caaattatcc
     6841 cgttcggcaa tccccaatcc gttttgccca taaacaccga ttgcgtgggc ttcatccaca
     6901 taaagataag tattgggaaa ctgttttttt aattggacaa gctgtttcaa atccgccaca
     6961 tcgccgtcca tactgaaaac agattcggta acgataaaag tgcggtcaaa ttttccgacg
     7021 tttttttcaa gcagattttt caaatgttca taatcattat gacgataacg gaaaaacgca
     7081 caccggctca aacggatgcc gtcaatcata ctggcgtgga caaatttatc tgccaaaatc
     7141 aaacttttcg tcgtcgtcaa agcaggcaaa ataccgagat tggcgtgata gccgctgttg
     7201 aacaataacg cgctttcccg ttggaaacgt tgtgcgacaa gctcttccaa atcggtataa
     7261 ataggaaagt tgcccgttaa taaacgcgat gaagaactgg taaaagaggg aaaattaccg
     7321 ccgtattgct gcaaaaaaga ccggcgcaag ttttcatctg atgccaaacc caaataatca
     7381 ttagacgaca tattcagcat tttgcggttt tcccgcgtaa tataccgccc ttgatgaatc
     7441 aaatccggaa tcgaacgata ttggttttgc gcgccgagtt gttcaagctg ttgtttaaaa
     7501 accttcatta tgatgtaaat attcctgagt taaagcctga accaacccag catcaaacaa
     7561 tcaagtaatg ccctgttaat aaaaaccgtt ttgccgccga cctttgccac acccctgcct
     7621 tcataatcca aggcggaaat ttccgcgaca tttgtttccg ttgccattac ccaatccgtc
     7681 cgcactgtac cgaagcgcat attttcgcac aaattggtac ggtttcaggt atgatttgcc
     7741 gaaaacctat cggacacaca ccatattttg atatgaataa aacaatctgc cgcactgctg
     7801 cactattgat aagcggtttt tcatacgcaa acaccgtcat tcccgatgtc tcccccgtcg
     7861 cacaaggtca acatgtcttc attaacatcc ctcagcaacg cctgttcctc tacaccgacg
     7921 gcaaactgac caaggtttat cccgttgcag taggtcgggc gatgacgcaa accaatctag
     7981 gcgaacataa aatcggtgcg aaagcctata accctgtttg gtacatcccc aaatccatac
     8041 agaaagaacg cggcgacggc gtaaaaacca tagccgccgg cccggacaac cctttggggc
     8101 cggtttttgt ccgcttaggc gaccccaaac tcggtttggg catacacggg accaatgcgc
     8161 cggccagcgt ccccggggtt cggagtcacg gctgcatccg catgaaatcg cccgacgcgc
     8221 tcgagtttgc caaaaccatc gccagcggct cgcccgcctc cgtcatctat caaatggcgg
     8281 ggctcaatga agatgcggat cgcaacctgt ggcttgccgc cttccgcgac ccttacggta
     8341 agaacaacct tgacatcgcc tctctgaaaa aaagtattgc gcaatgggca aaaacacagg
     8401 gtaaaaccat cgcgcccgag aaagtcgatg ctgtactcaa agaccgcacc ggatcggccg
     8461 tctgcctgac ctgcggcaaa aacggcaaga tgaagatgcc gctcaaatcg ctggcgtgga
     8521 tacagggttc ttcctcatac agccaagctg aagtcattga acaaactgag gaaaccaact
     8581 ctgccgaaat atccgagaca cgcacgcccg aagtgcctga tgtacacacg cccgaggcgc
     8641 aaccgcattt aaatacccaa tccgacggca cgccgactgc ctataccgaa ccggctgccg
     8701 attcatcgcc gcaagtagaa acacctgatc aggctgcttc cgagccggtt gatgtattat
     8761 tttcaataga tgtgatacgg cagggaaatt tgcgtttagg taattgaata accttctgat
     8821 tatttaatgc ttatgtttat cagaagttga taggcggttt ggtttttgtt tgacttttcc
     8881 tacaccgccg cctgccgctt tctgcaacat tcaagcgcac aaatatgcca tctgaaggct
     8941 ttagacggca tatttcacga taatagtcgc ccaatcaaat aaacacagat tggaaattta
     9001 ttttattttt cccttaccaa taccccagaa ctttccacca aatgctgccg ataacgaaaa
     9061 aaatcagaaa attgactacg ctcatgataa aacccgcctt ccaccattct cccattgtgg
     9121 tgtagcccga accgaaaatc acaggcgaag taccggtcgc ataatgagtg agggtcatca
     9181 taatgttgga tgcggccgcc atcatcagcg cggtcggcat cgccggtgca ttcagtgaaa
     9241 cggcagcagc gagaaatgcg ccgaacatag cggtaatatg tgcagtagta ctggcaaaca
     9301 tataatgcgc atacatataa gcaagcacga ggattacgcc cgcagccgtg ccgctaacgc
     9361 ccaaaccgcc gacactttcc gccaacactc cggagaacca tttaatcagt ccgagtttat
     9421 ttaaaaatgc ggccatcata atcaatgcgc caaaccaaat aatcgtatcc cacgcacttt
     9481 tttctttcaa aacatcgtcc caagtcaata caccggaaag caaaagcagg cttaatccga
     9541 taaatgcggt ggcggtggcg ttgatactaa aagcgtgatt gccggtaata agggcgggaa
     9601 catctgccca caacagcagc aagataccga aaatgaccgc cataatgatt tcgtctgccg
     9661 acattttacc catctccctc agacggtctt tggcaaattg aacggcattg ggcgtttctt
     9721 taatttcagg cggatacaaa aaatataaaa tcaaaggcat aacgaaaaag gcgataacgc
     9781 cgggaacagc cattgcccac gcccacgccc cccaagaaag acggaaacta ctgcctaaat
     9841 tttcggcaat caagttgacg attaaagggt tgggggcagt tgcagtaata aacatagccg
     9901 acgaaatggg attggaatga tagttgacca aagccaaata tttacccatc ttgccttctg
     9961 tgccttttgc gggattggag ccgtaactgc cggcaatcga ctgcataatc ggatgtataa
    10021 tgccgccgcc gcgcgcggta ttggaagggg taacgggagc cagcagcagt tcggaaagag
    10081 cgagactgta accgatgccc agcgtttttc ttccaaaaac ggcgataaac aaatatccga
    10141 tacgcatccc cagccctgtt ttgagcaaac cgcgcgaaat cataactgcg atggcaatca
    10201 gccaaatcaa cggattggcg aacgcactca acgcatcgct catcgccgcg cccggtttgt
    10261 cggcggttac gccggttact gcgaccaacc cgacggcaat aatcgacagc gcgcccaacg
    10321 gcatggcctt gccgataatg gcggcaatca caccgacaaa catggccagc agcgtccaag
    10381 cctgaggctt gaccccgtcg ggtacgggca gtgccaaaac cagggcgcac aatactgcgg
    10441 caatggcgag gggtatcggt ttgaaaccca atttcatcat attgacctcc gtaaaaaaga
    10501 ccgtcccgaa aaatcggaaa aataatattt aactaattgt tttataagat atattccgat
    10561 atttcaccgt ctttccgata tgcggctccg ggcaactttt gtttcagtat ttgaattttc
    10621 attagactga atacgccgtt tgaacggcac ggcgaaaacc cgggggatgc cggacgttca
    10681 gtcctttttc gcaccttgaa ggtaaagcat ttccaaggca attgtggcac cggccaaagc
    10741 ggtaatgtcg gattggtcgt aagagggggc aacttctaca acatccatac cgacgatgtc
    10801 gagatccgtc agcccacgta ggatttttaa tgccctgtcg ctgctcaagc cgccgcatac
    10861 gggcgtaccg gtcccagggg cgaacgacgg gtccaggcag tcgatgtcga aagtcaggta
    10921 aacgggcata ttgccgacgg tttctttgat tttacggacg gtctcttcaa cactgtcttc
    10981 attgacttgg ggggcggtca acacagtaaa aggcaatttt ttactgtgtt cggtacgtat
    11041 gccgatttgc acggaatgtg ccggatcgat gatgccttcc ttgggggcgg tataaaacat
    11101 cgtaccgtgg tcgtattcgc tgccgttgtc gtaggtgtcg gtgtgcgcgt caaaatgaat
    11161 cagtgcgagt ttgccgaaat agcgggcgtg ggcgcgcaac aacggtaggg taatgaaatg
    11221 gtcgccgccc aaactcaaac agcgtttgcc ggaagaaagt aatttgccgg cgtgcgcttc
    11281 cattttttcg acaaaatccc tgctgtcgcc aaaagaaaaa accaagtcgc cgcaatcaat
    11341 aatgttcagg cgttcgcgca catcaaatgt ccatggaaac ctgcggtgct cccaagcgag
    11401 gttgacggag gcgcgccgga tggcttcagg accgaaacgc gcgccggaac gccctgaaac
    11461 cgccatatca taaggcacgc cggtaataac ccaatcggca tgactttcat acggcataaa
    11521 attaagcggc aggcgcaaaa acccgaaatt attggaaacg agggagttgt cggtttgtcc
    11581 tgccagtgtg ctgtattgca tcgtaatgat tccttgtaat tggtttcaat cggtcgtgat
    11641 gattggtgtt tgagtaagaa aatcgggctt cagacgacat atccgatgcc ttgatgcgtc
    11701 tattcgtctt ccaaataggt ataaccatta agccccgctt cgagttcttt taagaaagac
    11761 atagcctgcg aggcaggaag gtctgaatgt tcgatttgtt cgcgatagcg tttcatcagc
    11821 tctttcggat cttgataaac gtattcgagc atatcggcaa cggtgtttcc ttcatcgtaa
    11881 tcgatgacgg taaattgtcc gtcttcccct acaacaacat cggcagtggc agtgtcgccg
    11941 aaaagattgt gcatattgcc gagtatttcc tgatatgctc ccaccataaa aaagcctaaa
    12001 agcggcggct cttcttcggg ataatcaggc ataggcatcg taccggcgat gccgtcgccg
    12061 tcgatgtagt ggtcaatcgt accgtctgaa tcgcaggtaa tgtccaacaa cacggcgcgg
    12121 cgcgcaatcg gttcattcaa accggtaatg ggacaaacag ggaaaagttg atctatgccc
    12181 caagcatcgg gcaaagattg gaagagtgag aaattgacat acagcttatc ggcaaaacgt
    12241 tcttgcaatt cgtcaataat ggttcggtga gaccggtgtt tttcattaaa caattcgccg
    12301 acttcatgac agatatttaa atacagttgc tccgcccacg cacgttgcgc caaactcaac
    12361 agcccgacat tatactgatt atgcacatcg gcaagatcaa actgcccttc gtgtatccag
    12421 ctgcgtaagg aacgtttttc ccgcgaggcg gaaatatccg tccaagtttc ccacatactg
    12481 tgcaacacac gcggcgcttc gggcgatggc gcatccagcc ggcgcggttt gtaacgttca
    12541 acgcctataa cattagcaac caaaacggcg tgatgtgcgg taatgccgcg cccgctctcg
    12601 gtgatgattg tcggatgcgg cagcccgtgt tcgagacaag cctgactgat gccccatacg
    12661 actgtggcgg catattcgtt gaggctgtaa ttaacggaac aatccgattg tgtgcggttt
    12721 ccttcgtaat ccacgccaag cccgccgcct acatcaaaac agcggatatt tacccccagt
    12781 ttgtgcaact caacataaaa ccgagccgat tcgtgtacac ctgtggcaac atcacggatg
    12841 ttcccaagct gcgagcctaa atggaaatgc aaaagctgca ggcagtccag cctgtttttt
    12901 tgtttcaaaa tatcgaccag ttgcaaaact tgggaagccg acaagccgaa ttttgatttt
    12961 tccccacccg aagactgcca ttttcccgaa ccttgggaag ccagtctggc gcgcacaccc
    13021 aaacggggct tgatgccgag tttttccgcc tcttccaata ccatttgtat ttcggacagc
    13081 ttctcaatca ccaaataaac ctgatgcccc agtttttcgc ccatcaaggc gaaacggata
    13141 tattcacggt ctttatagcc gttgcagacg attaatgttt gccgggtgcc ggcgtgtgcc
    13201 aaaaccgcca tcagttcggc tttagaacca gcttccaaac catgcggttg tccgcttgac
    13261 ataagcgatt cgatgacgcg gcggtgttgg ttgaccttga tagggtaaac caaacaataa
    13321 ccgcccttat agccgcactc ttcccgtgcc gtctgaaagg cgcggttaat gtcgcggagg
    13381 cggtgttcga ggatttgcgg aaaacaaaac aaaacaggca ggcgcgcctg atgtttttgt
    13441 tgcacggctt cagtcagttt ttgcagtgaa acaatttgat tgtgttgcga gggattgggg
    13501 cggacgatga tttcgccaga atcgtcaaca tcataataac ctatgcccca atgattaatg
    13561 ttgcacactt cacggatggt aaggataggc ataataaacc tgctccgtct gtcgtgttga
    13621 aaaggaatga ttataacaaa tcagcgtgaa atgtcatttt tttaataaga aaagcctgcc
    13681 tcatacctga taaggaacag gcaaaatgcc gtctgaacgc ttcagacggc attttggttc
    13741 agcttgccat caaaggagtc agcgatcgag ctgctctttg atgattttca ggtcggggta
    13801 agacaacacg atgtcgtatt cgcggccgtc tttataggct tccacttcca aaacaggttt
    13861 gccccaatgg tcgtcggcat cgacatcgtg aacctgataa ccgcgctgct ctaacatttt
    13921 cacggctttt gtgcggtttt gttcaaaatg gggatcaccg taaatctgac gctcggcaga
    13981 gtcgccggca aatgcggcag cggcactcag agaaacaacg gcagccaata acagtttttt
    14041 cattttcagt ccttttttta tcggttgatt gaacaagatg tgtttttcaa taccgccatt
    14101 aaaacacagc aaaattaggt ttgaattaga gggaagtcaa gaagcgtaaa tgccgtctga
    14161 aaaaaacaga gccgtcaaac ggctccgttt tccttatgct tctttggttt cagccacctt
    14221 ttggcgcagg cgcaggctca attcgcgcaa ctgcttgtcg tccacgctgt tgggggcatt
    14281 ggtcagcaga cattgtgcac gttgtgtttt cgggaaggca atcacatcgc ggatagattc
    14341 ggcgccggtc atcagggtaa ccagacggtc gagaccgaat gcaaggccgc cgtgaggagg
    14401 tgcgccgaat ttcaggttgt ccaagaggaa gccgaatttt tcttgttgct cttcagggct
    14461 gattttcagc gcggcaaaca ctttctcttg tacgtctgcg cggtggatac ggatagagcc
    14521 gccgccgatt tcccagccgt tcaataccat gtcgtaggca cgtgccaaac aatttgccgg
    14581 atcggaaacc atcaggtctt catgaccttc tttaggcgcg gtaaacggat ggtgtacggc
    14641 aacgtagcgg tcggcttctt cgtcgtattc gaacattggg aagtcgacaa cccacaaagg
    14701 tttccattcg tctgtgaaat agccgttgtc tttgccgtgt tccaaaccga ctttgatacg
    14761 caatgcgccg atggcttcgt tcacgacttt ggctttgtcc gcaccgaaga agatgatgtc
    14821 gccgttttgc gcgccggtac gcgcaatgat ttccttcagg gcgttttcag acaggaattt
    14881 cacaatcgga gattgcagac cgctgtcttc gccgttggag aggttgccca catcgtttac
    14941 tttgatgtat gccagacctt tcgcgccgta gatgccgaca aatttagtgt attcgtcgat
    15001 ttctttgcgg ctgaatttcg cgccgttcgg tacacgcaga gcgactacgc gaccgccttt
    15061 catgtcggct gcgccacgga agactttgaa ttcttccgtt ttcatcaggt cggtcaactc
    15121 ggtgaatttc aagttgatac gcatgtccgg tttgtcagag ccgtagtaga acatggcttc
    15181 agagtaaggc atgcgtggga aatcgcccaa gtccacgccc aatgcatctt ggaagacttg
    15241 tttcgccata ccttcggtga tgtccatgat ttcatcctcg ttcaagaacg aggtttccaa
    15301 gtcaatttgc gtgaattcag gctgacggtc ggcacgcagg tcttcgtcgc ggaagcactt
    15361 ggtgatttgg tagtaacggt cgaaacccgc caccatcaac agttgtttga ataattgcgg
    15421 cgattgcggt agcgcgaaaa actcgcccgg atgaacgcgg ctcggcacga ggtagtcgcg
    15481 cgcgccttca ggcgtggagc gggtcagcat cggcgtttca atgtcgatga aaccttgcgc
    15541 gtccaagtag cggcgaacgc ccatagccac ttggtaacgc aggcgcaggt tgcgctgcat
    15601 caccggacgg cgcaagtcga taacgcggtt ggtcaggcgc acgttttcgc tgatgttttc
    15661 atcgtcgatt tggaacggcg gcgtggcggc agcgttcaag acttcgattt ctttggcaag
    15721 aatctcgatt ttgccggaaa tcattttgtc gttggtcgta ccttcgggac ggttgcgtac
    15781 gcggccggta atgctcaaaa cgtattcgtt gcgagcggaa tcggcagcgg caaacgcttc
    15841 gggcgtgtcg gggtcaatca cgacttggac gatgccttcg cggtcgcgca ggtcgataaa
    15901 aatcacaccg ccgtggtcgc gccgacggtg tacccagcct ttgacggtaa cggtttggtc
    15961 taagtattgc tcactgatca gaccgcaata gttggtacgc ataaaatcac cttttattga
    16021 tttaaactga aaacagaaaa tgccgtctga acggcggctt tattgttgtt cgggcaaatc
    16081 cgccttttca gacggcatag gtcctgccaa tgttttgacg ggcaggtcgt cagggatgac
    16141 catacccagc gaaatcacat atttcaacgc ttcgtccacg ctcatatcga gttcgcgcac
    16201 atcgcttttc tttaccataa tatagtaacc gccggtcggg ttgggcgtgg tcgggacata
    16261 cacggaaaga taatcgccat cctgcggcaa tgcggcctta accgcattcg acacctgacc
    16321 ggacacgaat gcgattgtcc aaatacccga ttggggaaac ggcacgagta ctggtgtttt
    16381 aaacgaacgg ctgctgtcgg acagcagcga ttcggatact tttttcacac tcgaatagat
    16441 ggacttcaca accggaatcc gccccaacaa gctgtcccac gcggcaagaa tctgccggcc
    16501 caatacgttt gcggcaaaca atccggttac aaacaatacg gcaatggcaa cgataacgcc
    16561 cagccccggg atattaaacc ccaaaacata ttgcggccgc cattgcttcg gcagcaggtt
    16621 gacgagctga tcggacgcgg aaacgatata ggaaaccacc caaaccgtta ccgcaatcgg
    16681 cagccagacc aaaatgcccg taatcagata tttttttaac gccttggcag ctttaccgcc
    16741 ttcggccgca ggttccgtca tcttgcttga ttccgacaaa gtccgtacaa accgcacatt
    16801 atacgcgttt gcccggattc aaacgaaatt tttatcccgc cccgccaaac cgccggcggc
    16861 ttcagacggc acggcaactt gatataccgt ctgaacacgc ggttcagatg ccgtccaagt
    16921 cgttgaacat caacccgata ccgataccgt tctgcttgtg gttgtagtcg atcagactct
    16981 cgccgtaacc gtggaatccg cgtaccacgc ctttgagttt gcccttaatc ggaaacgtgt
    17041 aggcggcttc aatcgcgccg tagcccgttt tggggttgta gcgcaatacg gaatacacat
    17101 tctgcctgtc gttcaggcgg tactgcagct tcacgtcgcc ataccccata tagtcggcaa
    17161 tatcgggatt gtcgttttta tcgccgctct gatcgaacgc acgcacccac acgcgcggaa
    17221 tcaccgtcaa tttgccccat tccatgcctg ccatggcata aatcctgttc cacgaacgcg
    17281 attcgggacg gctctgtccg ttggactggt ggacaaaacc tgcaccgagc atacgcagcc
    17341 tgccgccgaa cggcaaatct gccttcacag gctgggtcag gaaaatttca ggtttgtaat
    17401 ccgtattgcg gaacggcgcg gatttcctgc cttggttgta aatctgccaa tcggatcttt
    17461 gggtgtagcc gaaccacaga tccgcgcggg ttttaaacaa atcttcggca attttgcttt
    17521 tgaacgaaac ctgcaatttg gtttccgcac gtttctgctg tccgaatttt tcctgtacag
    17581 tcgtaccgcg cgtcggcgaa cccggggcat agttgggcga attgttgtac cagagcggca
    17641 taaggtacat cggattgtgt tcgcgtacgc ccaacagccc gcgcaaatcg tttttatcca
    17701 aatcgtacat caggctcaaa ggcgtataga tgtcggcggt ttcgcccgca ctgtcggctg
    17761 gaagcgcatc cccgcctttt tcaacaacaa tgaccgcctc gcccttatcc aggctgctgc
    17821 ggacggtttc cgtaagattg agtacggctt tcgactcccg cccttcctgc cctgccgaag
    17881 acggaagctg tgccgcaaaa atcctgtcgt aacacgccaa acgcgtaaca ttgtccgtca
    17941 aagcggcgca ttgcagcgcg gtctctccaa aagcggatgc cgtcggcaac agtcctgtca
    18001 aaaggatata gcgcatattc cgtatattca tctccacccc cattgtcggc atattggttt
    18061 tcagacggca ttttatagcg gattcggata aaaaccgcac cctttccgcc atttcgggat
    18121 tttgcccccg caatacagaa aaccccgaaa ccgtcaggct tcagggtttt ccgcttatcg
    18181 cgtatcaacc gccttggcgg ttttgcaaaa atcaagccag tgcttttact tttgcagaca
    18241 gacggctttt gtggcgtgcc gctttgtttt tgtggaacac gcccttgtcg gcgatgcggt
    18301 cgatgacttt gacggactct tggtaaaccg cttgtgcggc agctttatcg cctgcttcga
    18361 ctgctttcaa tacttttttc actgcagtgc ggaatgcggt acgcaggcta gcgttgtggg
    18421 cgcgttgttt gacggactgg cgggcacgtt tgcgtgcttg tgcgctgttt gccatattgg
    18481 aatctcctga aagaaaaata aattctgtaa acgcgcaatt ttaaaggggg tttacattat
    18541 atgcaagcct tttctctcta tccgcccgtc ggacaccctg acagcgcaaa aaaacggcaa
    18601 aatcgggcaa ccgttttcct ccccttttaa aaacaggata attttttagg gaaatcagtc
    18661 caatatcact ttgagtttgc acaaatttac tttacaatgc ccaaaccttg cgccggggtg
    18721 ttatatgggg cgcggggttt tctttttttc caacctcttc catcaggaat ctgcccgtat
    18781 gaaaaaatcc gtattagccg ttttggccgc gctgtctttg gcagcctgcg gcggcagcga
    18841 aaaaaacgcc gtgcaacctc aagcagacgc tgcgtctgct gccaataccg aagccgctgc
    18901 caccgatact ctgaacatct acaactggtc gaactatgtt gatgagagta cggtcgaaga
    18961 ctttaaaaaa gccaacaatc tgaagctcac ttacgacttg tacgaaaaca atgaaacgct
    19021 ggaagccaaa atgctgaccg gcaaatccgg ttacgatttg gttgtgccgg gcatcgcctt
    19081 cctaccgcgc caaattgagg cgggcgcgta tcaaaaagtc aacaaagacc tgattcccaa
    19141 ctataaaaac atcgatcccg aactcttgaa aatgctggaa accgccgacc cgggcaacca
    19201 gtatgccgtc ccctatttct ccggcgtgaa cacgattgcg attacggcga agggcaaaga
    19261 gcttttgggc ggcaagctgc ccgaaaacgg ctgggatttg ctgttcaaac ccgaatacac
    19321 ccacaagctg aaatcctgcg gcatcgccct gtgggacacc ccgagcgaaa tgttcccgat
    19381 tttgctgaac tacttgggca aagaccccaa aggttcgaat cctgaagatt tgaaggcagc
    19441 ggcggaagtg ttgaagtcta tccgtccgga tgtcaaacgt ttcagcccgt ccatcatcga
    19501 cgagctggcg cgcggcgaca tctgcctggc ggcaggcaac ggcggcgatt tgaacttggc
    19561 gaaagcacgt tccgaagaag tgaaaaacaa cgtcggcatc gaagtgctga atccgaaagg
    19621 catgggcttc tggattgagt cttggctgat tcccgccgat gcgaaaaacg tcgccaatgc
    19681 ccacaaatac atcaactaca cgctcgaccc cgaaatcgca gcgaaaaacg gcatcgccgt
    19741 aacctttgcc cccgccagca aaccggcgcg cgaaaaaatg cctgccgaac tggtgaacac
    19801 ccgttccatc ttcccgaacg agcaggatat gaaagacggt ttcgtgatgc cgcaaatgag
    19861 tacggatgcg aaaaaactgt ctgtcagcct gtggcagaaa atcaaagtcg gcaccaactg
    19921 atttgaagca ttaaaaatgc cgtccgaaca tcgttcggac ggcattttat attggattga
    19981 aatagaaata tttatatagt ggattaacaa aaatcaggac aaggcgac
//
LOCUS       DAWFJR010000029        19773 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWFJR010000029 DAWFJR010000000
VERSION     DAWFJR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19773
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.029"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..455
                     /locus_tag="WGR04_000978"
     CDS             <1..455
                     /locus_tag="WGR04_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0794550.1"
                     /translation="ENNGNRIELKTENQGNGSFHAASSLGLSAIYDFKLNDKFDKFKP
                     YIGARVAYGHVKHQVHSVETTTETIFTTPTKGAKVGGKIPNTPSSKPPYHQSHSISSL
                     GLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYHF"
     gene            complement(429..638)
                     /locus_tag="WGR04_000979"
     CDS             complement(429..638)
                     /locus_tag="WGR04_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794551.1"
                     /translation="MPSERLSDGIFPCVLNRIHETPDAFGGNVGCRAMFGGDQRTKYA
                     GKGLMPSEGSDGIGVGESEVVAHAQ"
     gene            complement(656..1213)
                     /locus_tag="WGR04_000980"
     CDS             complement(656..1213)
                     /locus_tag="WGR04_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0794552.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(1289..2923)
                     /locus_tag="WGR04_000981"
     CDS             complement(1289..2923)
                     /locus_tag="WGR04_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ0794553.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2997..3326)
                     /locus_tag="WGR04_000982"
     CDS             complement(2997..3326)
                     /locus_tag="WGR04_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794554.1"
                     /translation="MSRCCLKRLQTASAVSAFSGGVLEVVRLCCRRLSVLFNPSVMLF
                     VPDCPVGGAAGFFQYEMLCPFFWQGRLQTGSNLAYDVFMSAGRLNGGRNPPHSRRFLA
                     LLCSVAL"
     gene            complement(3326..4432)
                     /gene="serC"
                     /locus_tag="WGR04_000983"
     CDS             complement(3326..4432)
                     /gene="serC"
                     /locus_tag="WGR04_000983"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ0794555.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNREGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4797..5228
                     /gene="rimP"
                     /locus_tag="WGR04_000984"
     CDS             4797..5228
                     /gene="rimP"
                     /locus_tag="WGR04_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ0794556.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKESGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            5256..6758
                     /gene="nusA"
                     /locus_tag="WGR04_000985"
     CDS             5256..6758
                     /gene="nusA"
                     /locus_tag="WGR04_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ0794557.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDYLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6770..9658
                     /gene="infB"
                     /locus_tag="WGR04_000986"
     CDS             6770..9658
                     /gene="infB"
                     /locus_tag="WGR04_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ0794558.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKTAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGSKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGLNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARSL"
     gene            complement(9869..11263)
                     /locus_tag="WGR04_000987"
     CDS             complement(9869..11263)
                     /locus_tag="WGR04_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HEZ0794559.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELIILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLIDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSGKYSEL
                     NLNQDKAASRRQYK"
     gene            complement(11337..12677)
                     /locus_tag="WGR04_000988"
     CDS             complement(11337..12677)
                     /locus_tag="WGR04_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ0794560.1"
                     /translation="MLNPSRKLVELIRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRETLERVRAGSFWLWVAAATFAFFTGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWAVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRAAQEGRLKDQ"
     gene            12820..13446
                     /locus_tag="WGR04_000989"
     CDS             12820..13446
                     /locus_tag="WGR04_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ0794561.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYVVMGWMVLAVMKSLTASLPLAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(13510..14928)
                     /locus_tag="WGR04_000990"
     CDS             complement(13510..14928)
                     /locus_tag="WGR04_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0794562.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDYIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKG"
     gene            15255..15983
                     /locus_tag="WGR04_000991"
     CDS             15255..15983
                     /locus_tag="WGR04_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ0794563.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(16042..16530)
                     /locus_tag="WGR04_000992"
     CDS             complement(16042..16530)
                     /locus_tag="WGR04_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ0794564.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRGRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGIALPVWSVAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(17288..17752)
                     /locus_tag="WGR04_000993"
     CDS             complement(17288..17752)
                     /locus_tag="WGR04_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEZ0794565.1"
                     /translation="MKELDKIDFRILKILQQNARIPMMELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQAKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            18077..19135
                     /gene="alr"
                     /locus_tag="WGR04_000994"
     CDS             18077..19135
                     /gene="alr"
                     /locus_tag="WGR04_000994"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ0794566.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWSAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGITEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
BASE COUNT         5224 a         5410 c         4963 g         4176 t
ORIGIN      
        1 aagaaaacaa tggcaacagg atagaactga agacggaaaa tcagggaaac ggcagcttcc
       61 acgccgcttc ttctctcggt ttgtccgcca tttacgattt caaactcaac gataaattcg
      121 ataaattcaa accctatatc ggcgcgcgcg tcgcctacgg acacgttaaa catcaggttc
      181 attcggtgga aacaacaacc gagactattt tcactacacc aacgaaaggc gctaaagtgg
      241 gaggcaagat cccaaacaca ccgagtagca aacctcccta tcaccaaagc cacagtatca
      301 gcagcttggg tcttggtgtc atcgccggtg tcggtttcga catcacgccc aagctgacct
      361 tggacaccgg ataccgttac cacaactggg gacgcttgga aaacacccgc ttcaaaaccc
      421 acgaagtctc attgggcatg cgctaccact tctgattccc cgacaccgat gccgtctgaa
      481 ccttcagacg gcatgagacc tttgcctgcg tacttggtac gctggtcgcc tccgaacatg
      541 gcgcgacacc cgacatttcc gccgaacgca tcgggcgttt catgaatccg gtttaaaacg
      601 catggaaaaa tgccgtctga aagcctttca gacggcattg tgcttgagat tccgtttacc
      661 aatgactgac aaacgcttcc aaatcggtat tcttgggctt atgcacttcc tctgtcggcg
      721 tgccgaccat catcagcccg atgattttat ccttatccgc acatccgaaa gcctcacgca
      781 acagggggct gttgacccac atccccgtta tccagacatt gtcgaaacct tgcgccgttg
      841 ccgccagttg cagcgcatac gccgcacaac ccgccgtcag catctgctcc cattccggtt
      901 tcggcttagg cacatcgcgg ttcggcgcaa acgttacccc gataaccatc ggcgccatat
      961 tgcccacttt ttccgccttt ttcatcgcat cgtcgccgaa attcaattcg gcaaccgttt
     1021 gctttaacac atcgcgaaaa cgttgcaatc ctacctcgcc ttgaatcacg gtaaaacgga
     1081 aggggcgcat attgccgtga tcgggaactt gggttgccgc ctgaaatatt tgttccaact
     1141 ccgccgcatc gggggcgggg tgcttcagct ttttggaaga tcggcggttc gtcaataatt
     1201 ttaaagcatc catatcgtta tactccggtc atcggtcggg tgttcggaca catatgccgt
     1261 ccgaaggctt cagacggcat atccggcatc agcgcggacg gcggcaggct gccaatatat
     1321 ccatttcctt ccgataggtt tggctgttgg aaatgtccat cagccccaat accgtgctga
     1381 aatagtggtc gtgcgaatat tcgttttccg ccgctttttg tttgaggcat tggaaatcta
     1441 tgcccccgtg ttggcggaag gctttggaaa accacataac catcgggata tgcgtctgcc
     1501 cggaaggcgc gatggcgtaa ggcgcggcgt gcaggtacat cccgttttcg cccaaacttt
     1561 cgccgtggtc ggaaacataa tgcaccacgc tttccaaatc gtcgcggttt tcaagtttgc
     1621 ggataacctt gtcgataaac tggtccacat acaaaaccgt attgtcgtaa gtgttgacca
     1681 gcgtggcgcg ggtgcatttg ttgatttcgt tggtgtcgca ggtcggcgtg aatttgcgtt
     1741 cggcttcggt atagcgttcg taatacgtcg gcccgtggct gccgatggta tgcaggatta
     1801 aaaccgcgtc tttatcgttt ttgttgagga cttcgtcgaa cttagtcagc aggatattgt
     1861 cgaggcactc gccgttgcgg cagtattcgg gcaggttgag cgaggtaacg tcggtattcg
     1921 gcactttgcc gcacacgccc ttgcagccgg aatcgttttc caaccaggta acttccacgc
     1981 cggcgcgctg cacgatgtcc agcaggttgt cttggtgttc ggctttgatt tcgtcataat
     2041 ccgtgcggtc gaaggttgag aacatacacg gcagggagtg cgcggtcgat gtgccgcagc
     2101 ttctgacctg cgggaaattg acaatttcat cgccgcgcgc ggcaagcagc ggcgtagttt
     2161 ggcggctgta accgttcaaa ccccagttgg cggcacgcgt ggtctcgccc acgaccagca
     2221 ccacgaaacg gcgcaggctg ccggccggcc ggttttgcac gaccgccata tccaattgcg
     2281 tataaggaat attggaacgc ttccaatctt tgtatttcga cacgcccgcg ccgatgaaat
     2341 tagacggcac aatcagatgg gttactgatt tattgttgcg gaaaaacgag gcgtaatcct
     2401 gatattgcaa cattgcgatg cccaacgcgc acaaaaagga aacggcggca agcacaaggc
     2461 gtgtcaaaag ctccttatac caaacgcggt atttaacctt gacggcgata tacgccagcg
     2521 cgggcaatac gcccaaacat acaatccaca gcacatagcc cggcgtaatc aggcgtgcgc
     2581 tttcggcagc cgtagtttgc aagacattat tcaacatcga cttgttgaaa tagatattga
     2641 aaaatatttc ttggtaagac accgccgcac tgataaccaa tatcaacgga atcaatacct
     2701 tatgcacgaa aggcagggca atgacgtgaa aaacgaaatt acttaaaaaa aacagcacca
     2761 ccggcatcgt atagaggaag atatccgccc cggtgccgtt aaaaggatga agctcgacaa
     2821 ctttggcaaa aaaggcgtaa ttcaatacca gcgaggaata cagggaaagg aaggcaatca
     2881 gcgcggaaga gccgagcttc ggcctcaggt tcggttttat catttggaat gtgtcggata
     2941 agggttggaa aaggcatccg gcatttggaa tccggattat tgaaaaagat tcttaattat
     3001 aaggcaacgg agcaaagcag ggcaagaaaa cggcggctgt gcggggggtt ccgcccgcca
     3061 ttcaaacgtc cggcagacat aaaaacatcg taagcaagat tcgaaccggt ctgcaaccgc
     3121 ccctgccaga aaaacgggca aagcatttca tattggaaaa acccggccgc gccgccgacg
     3181 ggacagtccg gcacaaacag catcacgctc ggattgaaaa ggacggatag ccgccggcag
     3241 cacaatctta ccacctccaa aacgccgccg gagaacgccg aaacagccga tgccgtctga
     3301 agccgcttca gacaacatcg ggacatcaac cgtaacgccg ttggaaatcg cgcataaaat
     3361 ccgccaaagc ccgcacgcct tcaagcggca tcgcattata aatgctggca cgcataccgc
     3421 cgacggtttt atagccctta agcaggcaca agccctgcaa ttcggcttcc agcacaaaac
     3481 ggcggtcaag ctcctcatcc cccgtttgga acacgacatt cattttagaa cgcgcattcg
     3541 gacggatacg gttgatatag aaaccgccgc tgccgtctat cgtctcatac aaggtttgcg
     3601 ccttcagccg attgaccgct tcaatttttt tcacaccgcc ctgcgcctgc agccagcgga
     3661 acaccagccc cgacatataa atcgcgtaag ttgacggcgt gttgtacata ccttcgcggt
     3721 tcaaatgcga acggtagttg aacacatcgg gaatatcgtt cggacaacgc tcgagcaaat
     3781 cctcacgcac aatcaccacc gtaacccctg ccggcccgat gtttttctgt gcgcctgcgt
     3841 aaatcagtcc gtagtcggca acatcaaact cgcgcgacaa aatctcgctg gacatatcgc
     3901 acaccagcgg cggcatgcct tctgaaaggc gcggcacttc acggtattgc agcccgttga
     3961 ccgtttcatt gacggcaaaa tggacaaacg ccgaatcggg tgcaacatcc cacgtttcca
     4021 caggcggcag gtcgagatag tcgaactgct cgccgccatg cgccgccaaa cggatttccg
     4081 tatcggtcaa acggctcatc tgttcataag cgatacggct ccagttgccc gttaccaccg
     4141 cgtcggcagt gcggaaaccg tgtgccagat tcatggctgc catattaaat tgggttgttg
     4201 ctccaccctg cagaaacaat atcttatagt tgtcaggcac tttcaaaagc tgcctcaaat
     4261 cctgttccgc atgatgcagg atgctcaaaa acatttccga acggtggctc attgccatca
     4321 caggaaaacc cgtaccgttg tagtccaaca tttcctgccg cgccgtttcc aacacggctt
     4381 cgggcaatac ggcagggccg gcggaaaaat tgtaaatcgg ataaagagac atgatgcagc
     4441 cttgattctg aacaataacc cgcccgattt taggcttgca gcaggctttg tgcaaggatg
     4501 gaaaatacct gtcctccgcc cgattccatg ccgcccgaac acggaaaata atatcaatat
     4561 attgatttac aaacataaaa atcatgcacg cgacaaatag atacatttgt tttgtcaaca
     4621 atattcacga tttcccatta caaacctccc ttacacccgc ttttttccgt cccaaaaaca
     4681 caaaataaat caacactttc atttctccgc aaaagcggtt ataatcacgc cgatttttca
     4741 actttgacga aaaatggcca cggggccatt tttttattat gtacataggg agcagcatgg
     4801 atatccaaac catcctcgaa aaaaccctgc ccggcctggg ctacgaactg gtcgatttcg
     4861 aactgaccgc gcaaggaaca ttgcgcgtgt tcatcgacaa agaaagcggc attaccgtcg
     4921 aagactgcgc aaccgtcagc aaccacttga gccgcgtctt catggttgaa gacatcgact
     4981 acaaaaacct ggaaatttcc agccccggac tcgaccgccc cttgaaaaaa gccgccgact
     5041 tcgtgcgctt tgccggtcag aatgccaaaa tcaaaacccg cctgccgata gacggtcaaa
     5101 aaaactttat cggtaaaatc gaaggctgcg aaaacgatac cgttaccgta tccttcgacg
     5161 gcaaaaccgt acaaatcgaa ttgggcaaca tcgacaaagc ccgcctgcgc cccgaattca
     5221 aattctaaaa cacaacaata ttggagatgt tcaaaatgag tcgtgaaatg ttacagctgg
     5281 cagaagcact ggcaagcgaa aaaaacgttg atgcggaagt cgtcttccaa gcactggaat
     5341 tcgccctgtc taccgccgcc aagaaaaagg cagaccgcga acacatggac gtgcgcgtcc
     5401 aaatcaaccg cgacaccggc gaataccaaa ccttccgccg ctggctgatt gtcgccgatg
     5461 aagactatac ctatcccgat gtcgaaaaaa ccatcgagga aatccaagag gaaattcccg
     5521 gcactaccat ccaaatcggc gaatactacg aagagcagct acccaacgaa ggcttcggcc
     5581 gccaagccgc gcaaaccgcc aaacaaatca tcctgcaacg catccgcgat gcggagcgcg
     5641 agcaaaatct gaacgagttt ctcgccgtca aagaagacat cgtgtccggt acggtcaaac
     5701 gcgtcgaacg ccacggcatc atcgtcgaag tcgttgccgg caaactggac gcgctgattc
     5761 cgcgcgacca aatgattccg cgcgaaaact tccgcagcgg cgaccgcatc cgcgccctct
     5821 tcctgcgcgt cgaagaaatc ggcaacaccg gccgcaaaca agtcattctg agccgtactt
     5881 ccggcgattt cctcgtcaaa ctgtacgcca atgaagtacc tgaaattgca gacggcatgc
     5941 ttgaaatccg cgctgtcgcc cgcgacccgg gccagcgtgc caaagtcgcc gtcaaagcca
     6001 acgaccagcg catcgatccg caaggcacct gtatcggcgt tcgcggttcg cgtgtcaatg
     6061 ccgtcagcaa cgaattgtcc ggcgagcgca tcgacgttgt cctttggtcg cccgagcctg
     6121 cccaattcgt catgagcgcg ctctcacccg ccgaagtcag ccgcatcgtc atcgacgaag
     6181 acaaacacgc cgtcgatgtc atcgttgccg aagaccagct cgcgctcgcc atcgggcgcg
     6241 gcggtcaaaa cgtgcgcctt gcttccgacc tgaccggctg gcagctcaac atcatgactt
     6301 ccgccgaggc agacgaacgc aatgcggcag aagatgccgc catccgccgc ctgtttatgg
     6361 attacttgaa cgtggacgaa gaaaccgccg acgtactggt tcaggaaggt tttgcaacct
     6421 tggaagaagt cgcctatgtt cctgccgccg aactgcttgc cattgaagga tttgacgaag
     6481 aaatcgtcga tatgctccgc aaccgcgccc gcgatgccat cctgaccatg gcgattgccg
     6541 ccgaagaaaa actgggcgaa gtgtccgacg atatgcgcaa cctcgaaggc atagatgccg
     6601 atatgctccg cagccttgcc gaagcaggca ttaccacccg cgacgacttg gcagagcttg
     6661 ctgtggacga actgattgaa atcaccggtg taaacgaaga aaccgcaaaa gccgtcatcc
     6721 tgaccgcacg cgaacactgg tttaccgaag acaaataaag ggggtacaga tgagtaacac
     6781 aaccgtagaa caatttgccg ccgagctgaa acgccccgtc gaagacctgt tgaaacagtt
     6841 gaaagaagcc ggcgtcagca aaaacagcgg cagcgattcc ctgacgctgg acgacaaaca
     6901 gcttctgaac gcctacctga ccaagaaaaa cggcagcaac ggcggcacca tcagcatccg
     6961 ccgcaccaaa accgaagtca gcaccgttga cggcgtaaaa gtcgaaacac gcaaacgcgg
     7021 acgcactgtc aacattcctt ctgccgaaga attggcagca caggtaaaag ccgcccaaac
     7081 ccaagccgca cctgtccggc cggagcagac ggcagaagac gcggcaaaag cccgagccga
     7141 agctgccgca cgtgcagaag cccgtgccaa ggcagaagcg gaagcggcaa aactgaaagc
     7201 ggcaaaagca ggcaacaaag ccaaacctgc cgcgcagaaa cccaccgaag caaaagccga
     7261 aaccgcacct gttgcggcgg aaaccaaacc cgccgaagaa agcaaagcgg aaaaagccca
     7321 agccgacaaa atgccgtcta aaaaacccgc cgagcccaaa gaaaaagccg ccaagccgaa
     7381 acacgagcga aacggcaaag gcaaagatgc caaaaaaccg gcgaaacctg ccgcacctgc
     7441 cgtgccgcaa cccgtggtca gcgcagaaga acaggcgcaa cgcgatgaag aagcacgccg
     7501 tgccgccgca cttcgcgccc accaggaagc cctgttgaaa gagaaacagg aacgccaggc
     7561 acgccgcgaa gccatgaaac aacaggcaga acaacaggca aaaaccgcac aggaagccaa
     7621 aaccggcaga cagcgtcccg ccaaacctgc cgagaaaccg caggcagccg cgccggccgt
     7681 cgaaaacaag cctgtcaatc cggcaaaagc gaaaaaagaa gaccgccgca accgcgatga
     7741 cgaaggtcaa ggccgcaacg ccaaaggcaa aggcggaaaa ggcgggcgcg accgcaacaa
     7801 tgcacgcaac ggcgacgacg agcgcgtacg cggcagcaaa aaaggcaaaa aactcaaact
     7861 cgagccgaat caacacgcct tccaagcacc gaccgaaccc gtcgttcatg aagttttggt
     7921 tcccgaaacc attaccgttg ccgatttggc gcacaaaatg gcggtcaaag gcgtggaagt
     7981 ggtcaaagcc ctgatgaaga tgggcatgat ggttaccatc aaccaatcca tcgaccaaga
     8041 caccgccctg attgtggtgg aagaactcgg ccacatcggc aaacctgccg cagccgacga
     8101 ccctgaagca ttcttggacg agggcgcgga agcagtggaa gccgaagcat tgccgcgtcc
     8161 gcccgtcgtt accgtgatgg gccacgtcga ccacggcaaa acctcgctgc tggactacat
     8221 ccgccgtacc aaagtggtac agggcgaagc gggcggcatt acgcagcaca tcggcgcgta
     8281 ccacgttgaa acccctcgcg gcgtgattac cttcttggac accccgggcc acgaagcctt
     8341 taccgctatg cgcgcacgcg gtgcgaaagc aaccgacatc gtgattcttg tggtcgccgc
     8401 cgacgacggc gtgatgccgc aaaccatcga agcgattgcc cacgccaaag cggcaggcgt
     8461 accgatggtg gttgccgtca acaaaatcga taaagaagcc gccaacccag agcgtatccg
     8521 ccaagagctg accgcacacg aagtcgtgcc tgacgaatgg ggcggcgatg tacagtttat
     8581 cgacgtttcc gctaaaaaag gcctgaacat cgatgcattg ctcgaggcag tcttgctcga
     8641 agccgaagtt ttggaactga ccgcacctgt cgatgcgccc gccaaaggca tcatcgtcga
     8701 ggcgcgcttg gacaaaggcc gcggcgcggt tgccacattg ctggttcaaa gcggcacgct
     8761 gaaaaaaggc gatatgctgc tggccggtac ggcattcggc aaaatccgcg cgatggtcga
     8821 tgaaaacggc aaatccatta ccgaagccgg tccgtccatc cccgtcgaaa tcctcggctt
     8881 gtccgacgta ccgaatgcgg gtgaagacgc gatggtattg gcggacgaga aaaaagcgcg
     8941 cgaaatcgcc ctcttccgcc aaggcaaata ccgcgacgtg cgccttgcca aacagcaggc
     9001 ggcgaagctg gaaaatatgt tcaacaatat gggcgaaacc caggcccaat ctttgtcggt
     9061 catcatcaag gcagacgtgc agggctctta cgaggctttg gcgggcagcc tgaaaaaact
     9121 gtccacagac gaagtgaaag tgaacgtgtt gcacagcggc gtgggcggca ttaccgaatc
     9181 ggatgtcaac cttgccatcg cttcgggcgc attcattatc ggctttaacg tgcgtgcaga
     9241 tgcctcttcg cgcaaacttg ccgaaaatga aaacgtggaa attcgctact acaacatcat
     9301 ctacgatgcc atcgacgacg tgaaggcggc gatgagcggt atgctttccc cggaagagaa
     9361 agaacaggtt accggtacgg tcgaaatccg tcaggtcatc tccgtttcca aagtcggcaa
     9421 cattgcaggc tgtatggtta ccgacggcgt ggtcaaacgc gattcccatg tccgcctcat
     9481 ccgcaacaac gtggttatcc acacgggcga actggcttcg ttgaaacgct ataaagacga
     9541 tgtaaaagaa gtccgcatgg gcttcgagtg cggtctgatg ctcaaaggct acaacgaaat
     9601 catggaaggc gaccaactgg aatgcttcga catcgtcgaa gttgcccgca gcctgtaatt
     9661 cctttgcaaa taaaatgccg tctgaagcgt tcagacggca acgaaacggg ttctgtatca
     9721 tacagaaccc gttttttgtc gcaaatcggc ttcagacagc cctcttgcct tatcccgatt
     9781 tgaatctgac ttgccataca aacaggcttc agacggcatt atagtgaatt aaatttaaac
     9841 cggtacagcg ttggctcgcc ttgccgtact atttgtactg tctgcggctc gccgccttgt
     9901 cctgatttaa atttaattca ctatattttc ccgacaggcg gatttgaagt ttctccacat
     9961 atccggcgcg ttcggcgcgg ctggtttcgg ggcggtgcgt attgagcgac gaccatttcc
    10021 aatgactgcg ggctttgttg agttcgggcg ggagtctggc ggcgtcccac ggggctttgc
    10081 ggctgtgcag ctcaatatcc gactgtgccg cgtgtccgcg cgtttgcagg acgtggagca
    10141 gatcgagggc gcgggcggcg agcagggtca gggtttcggg gtcggtgtgc aaggtttggc
    10201 ggccggcgag tttgtcggaa atggtgcggg tattgggcag gatgccgccc aaaaagccgc
    10261 cgattgccgt acccaatccg agcgagccgc cgagtgtggc gatgtccagc cccaagccga
    10321 tgagtgcgcc ggttgccgcg cccgtgccgg tgcggatgcc gtattgtttg agcaattcgc
    10381 tgtcgaacgg gtcttggcgg aaggcttgcg gcatccagtc gccgccgtcg atttcgctgt
    10441 ggtagaaacg gtagagggca aacagccgct gctgcatctg ccgttcgagt tggcgtattt
    10501 ccgcctgcat ggtttgcagc acggtggcgg tatcctcgtt ttcgtccact tcctgcctga
    10561 aggcggcggc atcaattaaa aagtcggcga tttcgcggcg cgcttcgccg tccagccgct
    10621 gccattcgcg ccggcgcatg gctgtcaggc ggtcaagtgt gctgcgttcg ggcaacatgg
    10681 tggcgaggtt ttcccacagg cgtagttcgc cttcaaaatc aaaggcgacg gtgtcgaacc
    10741 ctgcgaaaac gtgcaggttt ctcctcgcca gcatggttgt ccacgattcg ggaggctgtc
    10801 cgccggtaaa gttgaacacg ggcataaccg gtttggcgca ccatgaaagg ataatcaatt
    10861 catcccgata tttgtcgaga acgggttcgc gcgcgtcgat gacgtacatt gccatatcgc
    10921 tttgcaagac ttgccgtaag actttggctt cctgattgaa atcatggtgc gcgccgtggc
    10981 tgccgagaaa ctgttgcagc cgttcgatgc cgtctgaacg attgtccgta tggttttcca
    11041 gccattccag cacgccgccc gcgtcttcga gtccgggcgt gtcgtacagg aaaaccagcg
    11101 tgtctgcgcc gtcgctgatg gcggcttctt cgacatgacg cgtggtcgat ggggcgtttc
    11161 tgacttcgcc gaaaccgctg tcgcgcaaaa gggtacgcag gagcgaggtt ttgccggtgt
    11221 tggtgtgtcc gacgacggcg agggaaaggg gttgtttgtt catgatgttt ttgaagaatg
    11281 gattttcaga cggtcttttt tcagaatggc ggcttaacag aacatttcaa gtgagtttat
    11341 tggtctttca aacggccttc ctgcgccgcc ctgtcaggct caagccacgc cgcgccgcat
    11401 tcggtcagcg cgttacgcca atgttccagc ttttccgaaa ggtcgtctga aagcccctgt
    11461 tccgccaaaa gctgcaccac cgcgccgccc tgcgccgctt ccgaaagtcg gacgatctgc
    11521 cgcaacacgc cgcggtcggg cacagtttgg gcgcgcacgc cgataagcag ttgcgccggt
    11581 ttctgcttca gctctgtctc cagcgcggca acctgttccc gattggcggc aacgccctta
    11641 tccagccatt cctgcgccag cctgccctcg aaccattcgc cgtcctgcca ttcggtctcc
    11701 agcatgaccg cccatttcgg cgcatcgttc aagacgattt tcggcgaaac ggcggacacg
    11761 gtttcccgac gcgtatccgc atcggtgatt ttgttctgcc agcggcggat gaccgcctga
    11821 taatagggtt tttccaaatc caatccgttt tcgcttgttt tcaaaaggat tttgcatacc
    11881 gcccaagcca agaggcgcgg caggatgccg tagcaggcga tactgccgac cagcagcccc
    11941 gaccaagccc gcgcatcggc aatattgccg ttcagacggc cttcgatgac cgcccgcgca
    12001 tcaggcacgg gaaaacccag tttcgcaggc agccatgcca acatttccac cagccgtacc
    12061 gaagacgaat cgcccaacag cgtgctttcc cagttgaacg tatattgccg caccaaaagc
    12121 agcaacaata ccgacaccag cattccgagc agcgtgcaga gccacaggct gtgcgacgtt
    12181 gcgcctattt tccaacgtac cgaaggttgc cgccactcgt ccgcatacag ccgcaacacc
    12241 gcctgattta cagggtcttt gccccgaaac cacgtcgctg gactgctgaa aaaacgcccc
    12301 actttcacgc gcaggaacaa cattgccaac catactgcca gcatcagcgt attcatgccc
    12361 aacacgcccg ccaaaaccaa aaagaaattc agaccctgat tgtccattag aagataagtg
    12421 cctgaaaaac cggtaaaaaa cgcaaacgtc gccgccgcca cccacaacca gaacgacccc
    12481 gcacgcacac gttccaacgt ctcccgcagc atacggttcc tgtcgatcat ctccgcccga
    12541 cggatgattt tttcctccgt actgccgtcc acgcggcgca aagcctccgt cgcctgcacg
    12601 ggatcgccgc tgaaaataaa accgccttcg tccaaaatac ggatcagctc aaccagtttt
    12661 cgggatggat tcaacataaa atgccgtctg aaaataaaaa acagatttta acacacgcat
    12721 tttcaagaat attcacagtg taggcaaaga gtaaatctca cacagaagca aaagtatcgg
    12781 cgtaaactga ctgcctctac tttcccgaaa gattgtgcga tgtatacagg cgaacgcttc
    12841 aatacttaca gccatttgag cggtttgatt ctggcggcgg caggtttggc gctgatgctg
    12901 ctgaaaacca taggacacgg ggacggctac cgtatcttca gcgtatcggt ttacggcatc
    12961 agccttcttc tgctctattt gagttcctcg ctgtaccacg gaattgcagc cggaaaactg
    13021 aaaagcattt tgaaaaaaac cgaccactgc atgatttatg tgctgattgc cggaagctac
    13081 acaccgtttg cactggtttc tttgagaaac gggccgggct ggacggtatt ttcactgtcc
    13141 tggctgctgg cggctgcagg aatcgcacaa gaactcacca tcggacggaa aagcgaaaaa
    13201 cgactgctgt ctattgcgat ttatgtcgtc atgggttgga tggtcttggc ggtaatgaaa
    13261 tccctgacag cctcactccc gctggcagga ctggcttggc tggcggcagg cggtatgctg
    13321 tacagcgtcg gcatttactg gtttgtaaac gatgaaaaaa tccgacacgg gcacggaatc
    13381 tggcatctgt tcgtattggg cggcagcatc acccaatttg tcagtgtgta cggttatgta
    13441 atctgaatgc cgtctgaaaa gcaaaacctc ccgatcctga agattgggag gttttctgtt
    13501 tgccggacat cagcccttgt cgtggaactc gtgaaattca tactgatagg acaaatcccg
    13561 acccgctttt ttctgtgcca aataatcatc ataaatggca cggatttcct tacgcaacaa
    13621 aaacagggct atcaagttcg ggataaccat aaagccgttg aacatatcgg acaggctcca
    13681 aaccaaatcg actttgccga gcgtacccaa aacgatggca agcagaacca atgcgcgata
    13741 gatgcccaag tgtcttcccc tgaaaagaaa acggatgttg gactcaccga aataatacca
    13801 gccgataatg gtcgtcaagg cgaaaaaggt cagacacacg gcaagcaatt gcgaaccgaa
    13861 gcccggaaat gccttgttaa aggcaaattg agtaaccgcc gcgccctgtt cgcccgaaag
    13921 gttggcatca gtcagcagga taatcaatgc cgtagccgta cataccaaaa ttgtatcgat
    13981 aaacacgccg acaaatgccg ccataccttg ctgaacagga tgcttcacat ccgcagtcgc
    14041 gtgggcgtgc ggagtcgaac ccatacctgc ttcgtttgaa aacagaccgc gcgccacgcc
    14101 gaaacgtatc gcttcacgca taccgatacc cgcagcaccg cccaaaacgg cttcaggatt
    14161 gaaggcggcg gtaaagatgt ggttgaacat cggcacaata tagtcggaaa attcaaacag
    14221 gataacgacg gcgcacaaaa tataaacaac cgccataaac ggcacgacaa actgggcaat
    14281 attggcaata cggttcacgc cgccaatcac aaccatgccc gcaaggacgg caagcacaat
    14341 accgactgcc aaagaaggca catcaaatgc aatggtaacg gcagaagcaa tggagtttgc
    14401 ctgtgtcgca ttaccgataa agcccaatgc gataatcaac gcaatggaga aaaagcccga
    14461 caggaaacgc gccgcgcccc tgccgatttt cggagtcaga ccgtgcgtaa tataaaacgc
    14521 cggcccaccg atgtatttgc cgtggctgac gacgcggtat ttctgcgcca gcagtgcctc
    14581 cgcaaaaatc gtggacatcc ccaaaacggc agaaacccac atccaaaaaa tcgcgcccgg
    14641 cccgcctgcg gtgatggcgg tcgccacgcc ggcgacgttg cccgtaccga tttgcgcgga
    14701 tatggcaacc gccaacgcct gaaactgcga taaagactta tcgtctttat cgcctttggc
    14761 aaacaagccg ccgaatacag atttgaatcc cgcacccagc ttggtaatct gcggcgcacc
    14821 gagatacagc gtaaaaaaca ggccgatacc caaaagcgcg taaatcagca ggtagtccca
    14881 aaggaaccga ttgactgtac ccactagaac agacaatata ttttccataa aataaacctt
    14941 atcttacaat taaaatgact gccttccaaa agacattcca ataaggaaac acggcgagca
    15001 gaccgtattt gccgcaacag atgccttaaa ttgtcaacaa tcggggagaa gctgcgccat
    15061 cataccgtaa aatatcgtta attaaaacat ttctttattt ttaagcggaa agcggaggaa
    15121 atcgctttca gacggcatcg acaacggcac ggcataaaac aggatatttt gggtacttgc
    15181 aacttatgtt aaaatgccga ccgtaaaaaa tctgacaaaa acagattaat tatttgaaat
    15241 aagaaaggaa atttatggca ggccatagca agtgggcgaa tatccagcat aaaaaagccc
    15301 gtcaggatgc caaacgcggc aaaatcttca cccgtttaat caaagaaatc accgttgcgg
    15361 cgcgtatggg cggcggcgat cccggtgcca acccgcgcct gcgcctggct ttggaaaaag
    15421 cagccgaaaa caatatgccc aaagacaatg tgcaacgcgc catcgacaaa ggtacgggca
    15481 acttggaagg cgtggaatac atcgagttgc gctacgaagg ctacggcatc ggcggcgcgg
    15541 ctttgatggt ggactgcctg accgacaaca aaacccgcac cgttgcggac gtacgccacg
    15601 cgtttaccaa aaacggcggc aacttgggta ccgacggctg cgtggcgttc aacttcgttc
    15661 atcaaggcta tttggtgttt gaaccgggcg ttgacgaaga tgcgctgatg gaagcggctt
    15721 tggaagccgg tgcggaagac gtggttacca acgacgacgg ttccatcgaa gtcattaccg
    15781 cgccaaacga ttgggcgggc gtaaaatccg ctttggaggc ggcaggttac aaatccgttg
    15841 acggcgacgt aaccatgcgc gcccaaaacg aaaccgaact ctccggcgac gatgccgtca
    15901 aaatgcaaaa actgattgac gcgctggaag acttggacga cgtgcaagac gtttacactt
    15961 ccgccgtatt gaatttggac tgatacgcag cacagcagac atacaataaa atgccgtctg
    16021 aacctttcag acggcattga tttatttagc ccttgcccac gcccaccaaa ccgtcaggac
    16081 aaccgccaga aaatacgcca cgctccaaac aggcaaggca attcccagca gataatccgg
    16141 ttcggcacaa tttccgaacc cgcgcacgac aggctcgaac caatcaaaca aaggccagcc
    16201 tttcaagcgg aacgtccacg gcgcgccgca cgaaggagcg gttcccggcg gcagcgactg
    16261 caaccacaac tgatatgccg caacagaaat acccgtaacg gcaggaatgc tgataaagac
    16321 agcaccgaac aaaccgcctg cccttcctct tggtctgcac atcaggacaa ttgccgtaca
    16381 caatgcggtt gccaaaacgc ataaccgctg actgatacac aaaacgcaag gctccatacc
    16441 caaaacatac tgtgccgcca aagaaccggc aaatgcacag accgaaacgg caaacagcag
    16501 ccaaacggct tttctaaata acggggtcat tttctcaaca caccaatcaa aataccgata
    16561 tgccgatttt gctggatata tcccgagatg gcaagggacg aatcgacacc ggcgcaggtg
    16621 cggacgattt cggttttccc gatatacaca agacctgaca agggacaaaa cggcgatttg
    16681 cccgcacaat gcccgcaaaa aaatacatcc ggaggatttg aatttcggca aatttagcga
    16741 atcaaaagtt tatttcaatg aaattatatg atttttttga ataagcggat tgacaggttt
    16801 ttgaaggaat ttgttaccgg atagccatcg ggcaagtttt ttgcaaaatt tgaatcggtc
    16861 gggtaaattt tcaaaaaata attgacagcg gataagaaac ggcggataat tcccgccgtc
    16921 gagttgcttg atgcagcttg atttttctcc tctatttctc ctttgtagac ttggcacaca
    16981 ttcaactgga tgtgtgcatt tttttatctc cgcttttttt gaaatttaat tacttttatt
    17041 gtttgaaatt ccattcttta agaaatttaa caagaaaaac gcttgctttt tgaccggaaa
    17101 agctgcataa ttcgttccgt tagctggttc gagtagtcag ttaatagttt ctcctctatt
    17161 tctcctttgt agacttggca cacattctat tgaatgtgtg cattttttta tctgaagcaa
    17221 caagcctctg tgcgtgatgt tgttatgttt catttaggtg tcaaaccgca tacccggtct
    17281 gaaatattca atccaaatcc aaaaccggat tttctttgac ctcctccatc acaacataac
    17341 tcctactctc cgaagcggca ggcagttgca ataggatatt gcctagcata tcccgatagg
    17401 cagacatatc gggcaaacgt actttaatca gatagtcgta ttcgcccgac accaagtggc
    17461 attccataat ttgcggaatt ttcagcactt cttttttgaa atcttcgaaa atattgcccg
    17521 atttggcttg cagcttcagc tcgacaaaaa ccaatagtgg tttgcccaac agatggggat
    17581 tgagatgggc gtgatagccg gaaatataat gttcccgctc caaacggcgc accctctctg
    17641 taacgggcgt ggtggacaag cctaccttct cggcaagctc catcatcggg atgcgggcat
    17701 tctgttggag gatcttaaga atgcggaaat cgattttatc tagttctttc atttggattg
    17761 ccttgtattt attattgatt ttaacaaata gagtatatag tggattaaca aaaaccagta
    17821 cggcgttgcc tcgccttgcc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg
    17881 atttttgtta atccactata tatttgggaa agcgattata tcaggaaaag caaaccgcct
    17941 tcctacctaa aaactgctgc ttcggcttga agatacaagg gtctttaata ttttaaaagc
    18001 cttgccgttg gattataatt ccctaattga tttcttaatt ttgctaataa acacttgttt
    18061 ggtaaggaat gaatttatgc gccctttgaa cgtgcagatc aggttgggca accttaggca
    18121 caattatcgg attttgaagg aaatgcacgg aggcaaactg ttggcggtag tgaaggccga
    18181 cgcatacgga cacggtgcgg tcagatgtgc tttcgcgctg gcagacttgg cagacggctt
    18241 tgccgtggcg acaatcgacg agggaatcag gctgcgggag agtggcatta cccatccgat
    18301 tgtccttttg gaaggcgtat ttgaagcatc agagtacgaa gcggtcgaac aatactcgct
    18361 ctggtcggca gtcggaaacc aatggcaact tgaggctttg ctttcccgtc attggaaaaa
    18421 acctgtcaag gtctggttga aaatggattc gggaatgcac cgtaccggtt ttttccctca
    18481 tgattacgct tcggcatatg cggcattgaa gcagtcggaa tatgtggaca gtattgtcaa
    18541 attctcgcat ttctcctgtg cggacgaacc cgaaagcggt ataacggaaa tacagatgga
    18601 agcattcgat ttgggtactg aagggctgga aggcgaagaa agccttgcca actccgccgc
    18661 tattttgaat gttcccgaag cacgcaggga ctgggggcgc gcgggcttgg cgttgtacgg
    18721 catttccccg ttcggaggca gcgatgacag gctgaaaccc gtgatgaggc tttccacccg
    18781 tattttcggc gaacgcgttt tacagccgca ctcccctatc ggttatggcg caacatttta
    18841 taccagcaaa tctacgcggg tcggcctgat tgcctgcggt tatgcggacg gttatccgcg
    18901 ccgtgcccca agcaattccc ccgtcgccgt cgacggtaaa ttgacccggg tcatcggcag
    18961 ggtctctatg gatatgatga ccatcgagct ggacgcttca caagaaggtt tgggacacga
    19021 ggtcgaactg tggggcgata cggtcaacat caataccgtt gccgaagcgg ccggaaccat
    19081 cccttacgaa ttgatgtgca atatcaaacg tgcaaaattc acttatatcg agtaatcgag
    19141 tccaaacaaa aatgccgtct gaagcctttc agacggcata aatgcgtttt gacaaaatca
    19201 gtatagtgga ttaacaaaaa tcaggacaag gcgacgaagc tgcagacagt acaaatcgta
    19261 cggcaaggcg aggcaacgcc gtactggttt aaatttaatc cattataaaa gacattgcca
    19321 aatagaaaat actgataatg cccacaatct gaaaccccat ccttcccgca aaaacggaaa
    19381 atcaaaatca gaaacctaaa atttcgtcat tcccgcgcag gcgggaatcc agttttttga
    19441 gtttccgtca tttctgataa attctcgtcg tttttcattt ctagattccc gcctgcgcgg
    19501 gaatgacaaa attaaagttt cagaatttat ttgaacaaca aactagattc ccgctttcgc
    19561 ggggatgccg gcggataggt tttgttgttt cggataaatt cctgttgttt tttggtttcc
    19621 agattcccac ctttaaaaga atgacgtaag aaagcagaaa taaggacaca gggatttttt
    19681 atagtggatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac agatagtacg
    19741 gcaaggcgag gcaacgctgt actggtttaa att
//
LOCUS       DAWFJR010000030        19412 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWFJR010000030 DAWFJR010000000
VERSION     DAWFJR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19412
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.030"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(1..99)
                     /locus_tag="WGR04_000995"
                     /pseudo
     CDS             complement(1..99)
                     /locus_tag="WGR04_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(256..3006)
                     /gene="gyrA"
                     /locus_tag="WGR04_000996"
     CDS             complement(256..3006)
                     /gene="gyrA"
                     /locus_tag="WGR04_000996"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ0794567.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIRLIKESPNAVEAKDKLLAHPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            complement(3104..3604)
                     /gene="hscB"
                     /locus_tag="WGR04_000997"
     CDS             complement(3104..3604)
                     /gene="hscB"
                     /locus_tag="WGR04_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ0794568.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            3686..3907
                     /locus_tag="WGR04_000998"
     CDS             3686..3907
                     /locus_tag="WGR04_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ0794569.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     VKKWRDGFDTVPPFLCLKHGSFRRSRRSL"
     gene            complement(3867..4187)
                     /gene="iscA"
                     /locus_tag="WGR04_000999"
     CDS             complement(3867..4187)
                     /gene="iscA"
                     /locus_tag="WGR04_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ0794570.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(4274..4660)
                     /gene="iscU"
                     /locus_tag="WGR04_001000"
     CDS             complement(4274..4660)
                     /gene="iscU"
                     /locus_tag="WGR04_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ0794571.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            complement(4920..6134)
                     /locus_tag="WGR04_001001"
     CDS             complement(4920..6134)
                     /locus_tag="WGR04_001001"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ0794572.1"
                     /translation="MTVKTPVYLDYAATTTVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILVSVMWANNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELAQDTAHYLKLRDI
                     FLKGIEGIEEVYINGDLGHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            complement(6163..6609)
                     /gene="iscR"
                     /locus_tag="WGR04_001002"
     CDS             complement(6163..6609)
                     /gene="iscR"
                     /locus_tag="WGR04_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ0794573.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNSDGSRVVQFTHIH"
     gene            6959..8131
                     /locus_tag="WGR04_001003"
     CDS             6959..8131
                     /locus_tag="WGR04_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ0794574.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            complement(8267..11050)
                     /locus_tag="WGR04_001004"
     CDS             complement(8267..11050)
                     /locus_tag="WGR04_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ0794575.1"
                     /translation="MANNKTLFEVIENERKAVKKYKPELLEMPEFTSKNLKYDFFEWQ
                     KSALENFLIFDRTSKLDDFPDLKNKPTHLLFNMATGAGKTMMMAALILYYFEKGYRHF
                     LFFVNQNNIVDKTENNFIDPTHAKFLFTEKILQGDTVIPIRKVETFSPHSDGIEIKFT
                     SIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEVELKAN
                     AGNAEIERKGWEHMVLELLLNKNGNPSQNVLLEFTATLPENADVQQKYADKIITKFGL
                     KEFLQKGYTKEINLVSSTLGKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKTI
                     DESKADYLAFLNWAENVQAVDFSFLTTFSTSLNDSDSDNANEQGKTRTEQALKFMQEK
                     GVEFAHLADWVKQNYQKHNVIITNSETNKTKTEKTDSETEKLLNNLEAADNPIRAIFT
                     VDRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFE
                     GKQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLA
                     TFKLKSEFAANKDFRELLIWANKKIPNPNAKTNNANSLQANPQTLSFQVHGNQLLQET
                     QFTADENDEKVKQLGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSKL
                     NIQNRNELQNKLLKDWQIEFLGLGQDKQVRLDDKLAGCLKILEMVEKHLNESDMPFIG
                     TKEFTPKKLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISAR
                     LGDLKSQYDVHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIF
                     IEPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTE
                     SFPLGAASLEKMK"
     gene            complement(11040..12413)
                     /locus_tag="WGR04_001005"
     CDS             complement(11040..12413)
                     /locus_tag="WGR04_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0794576.1"
                     /translation="MKLIYIDPPYNTGNDGFKYNDKFNHSTWLTFMKNRLEIAKELLM
                     EDGSIFVSIDDNEQAYLKILMDEVFGNENFICNFIWEKKTGASDAKQIATITEFVLCY
                     SKNFKTVKLNKNTFSYDTERYKLSDKFEQERGKYYIDNLDRGGLQYSDSLNFAIQCPD
                     GTFTYPNGRTEFVNDGWIWKWSKNKIDWAITNGFLEFRKSKSKKSGWSVCYKNYMLVD
                     NENKPIERSAPYKNLIQDILNTHATDELKKLFGSKVFTTPKPESLLQYLIQIATSEND
                     IVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIETLAVERLKKVIDGEQGGISKAVNWQ
                     GGGEFVYAELAPFNETAKQQILACEDSDGIKTLFEGLCERYFLKYNVSINEFSQIIEE
                     PEFQSLPLDEQKQMVLEMLDLNQMYVSLSEMDDEQFADCLNDDDKALSRAFYQSVKNQ
                     AEKKDGE"
     gene            complement(12613..13161)
                     /locus_tag="WGR04_001006"
     CDS             complement(12613..13161)
                     /locus_tag="WGR04_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794577.1"
                     /translation="MKTDIQTELTQALLPHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLSNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSINNSYTKYANRIGLTDGNRFL
                     KDSSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRNNSQSVSQSVSQSVSQSVSQSVSQS
                     VSQSVSQSIIHQINPKKTRNLF"
     gene            complement(13482..14405)
                     /gene="truB"
                     /locus_tag="WGR04_001007"
     CDS             complement(13482..14405)
                     /gene="truB"
                     /locus_tag="WGR04_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ0794578.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFRAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIAAARAD
                     ISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKPRDITVYAIDI
                     AEFDSPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLDETERDSLLLPCDVLVAHFPQTVLNDYAVHMLQCGQRPRFEEDLPSDTPVRVYT
                     ENGRFVGLAEYQKEICRLKALRLMNTAASAV"
     gene            complement(14438..14809)
                     /gene="rbfA"
                     /locus_tag="WGR04_001008"
     CDS             complement(14438..14809)
                     /gene="rbfA"
                     /locus_tag="WGR04_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ0794579.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            15000..16244
                     /gene="clpX"
                     /locus_tag="WGR04_001009"
     CDS             15000..16244
                     /gene="clpX"
                     /locus_tag="WGR04_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ0794580.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIAWQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGKEPELVFES"
     gene            complement(16309..17502)
                     /locus_tag="WGR04_001010"
     CDS             complement(16309..17502)
                     /locus_tag="WGR04_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ0794581.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTKGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALRSLGEQTGVFKQVRGMGLLLGCVL
                     DAPYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLDDEDTAEGLRRLEHVLTEFA
                     AANRP"
     gene            17906..18406
                     /locus_tag="WGR04_001011"
     CDS             17906..18406
                     /locus_tag="WGR04_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794582.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            18491..19045
                     /locus_tag="WGR04_001012"
     CDS             18491..19045
                     /locus_tag="WGR04_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794583.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     TFIGNALANKFTRAGNQDSPVARRARAAYHQSAHPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            19059..19259
                     /locus_tag="WGR04_001013"
     CDS             19059..19259
                     /locus_tag="WGR04_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794584.1"
                     /translation="MPSEGLSDGIFAFVRGIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
BASE COUNT         4465 a         4583 c         4977 g         5387 t
ORIGIN      
        1 taacgcctac taactctgct gtcgttcttg cagttacacc tgcgacaaac agttcaatga
       61 gtttatttgt ttataccggc ttagacgact ttttctcata ggggcaactc taacttaatt
      121 tgaatttccc tagttatcta ggacagcccc ttgtttttaa ttgactataa tccgctatat
      181 tgtgagaagc cggatgacgg atgagggata aaataaatgc cgtctgaatc ttcagacggc
      241 atcgggagat gattttcagt tctcgacttc cggttcggtt acattggaaa ttacagaagc
      301 gtcggagagt tcggattcgt cttcggcaac acgttccagc gataccaagg tttcgccttc
      361 gtccaagtta atcagtttca cgcctgctgc ggcgcggccg gtttcgcgga tttgttcgac
      421 tttggtgcgg ataagtacgc cgccgctggt aatcagcatc aaatcgtcgg tttcgccgac
      481 caaggttgcg gcgaccaaat cgccgtttcg ctcgccggtg ttaatggcaa tattgccttg
      541 cccgcctttg tttttgcggc tgtaatcggc aatcggggtg cgttttccgt atccgttggc
      601 ggtggcggtt aaaacttgca aaccgctttc ttcggtttca ggggcgaagg taatcaggct
      661 gacgattttg ccgtcggcag gcaggcgcat accgcgcaaa ccgccgctgc cgcgaccgga
      721 cgggcgaaca ccgtgtttgc cgctcggcag tgcgttttcg ctgtcggcgg tttcatcttc
      781 gatgccgtct gaaatttcgg tttcgatgtc ggcatcttcc gcttcgtcgt tgccggattt
      841 ttcccagtat tcgttgaagc ggatggcttt acctaagttg gagaacagca tgatgtcgtc
      901 cgcaccgcct gtttgcgcag cgccgacgag gtagtcgcct tctttgagcg cgatggcttt
      961 aatgccttgg gcgcggacgt ttttaaaggc ggaaagttgg acttttttca ccattccctg
     1021 cgcggtggcg aagaagacgt attggtcttc ggggaactcg cgtactgcca gaatcgcgct
     1081 gactttttcg ccttcttcca actggatgac gttgttaatc ggacggccgc ggctgttgcg
     1141 tccgccttcg ggcagtttgt aaaccttaat ccaatgacac ttgcccaaat tggtaaagca
     1201 catcaaataa tcatgcgtgt tggcaacaaa cagggtttcg ataaagtctt cgtctttggt
     1261 ggcagccgcc tgtttgccgc gcccgccgcg acgctgcgcc tgatagtcgg tggtcggctg
     1321 ggttttgata tagccgccat gtgtcagggt aacgaccatt tcgcgttgcg gaatcaggtc
     1381 ttcatcggca atgtcgccac cgaacgggtt gatttcgctg cggcgttcgt cgccatagtt
     1441 ggttttgatt tcttccagtt cgtcgcggat gatttgggta atgcgttcgg gtttggagag
     1501 gatatccaca aagtcgatga ttttacccat caggtttttg tagctttcga caatttcttc
     1561 ttgatcgagg ccggtcaggt ttcgcaggct catgcgtaaa atagcatctg cctgaatctc
     1621 gctcaggtaa taaccttgct ctttcaagcc gatgtttgca gccaatcctt ccggacgcat
     1681 catttccaaa tccagaccgg aacgcgtcag catttcttca acgaggctac tgcgccaagg
     1741 gtgcgcaagc agtttgtctt tggcctcgac cgcgttgggc gattctttga taagccggat
     1801 gatttcatcg atattggaca gtgcgaccgc tttgccttcg gcgatatgcc cttcatggcg
     1861 tgccttcttc agccggaaaa gcgtacgtcg ggtaacgact tcgcggcggt ggcgcaggaa
     1921 ttcggagaga atctgtttca ggttcaacag gcgcggttgt ccgtcgacca aaaccaccat
     1981 attgatgccg aaactgtctt gcagcggagt cagtttgtag agttggttta agacgacttc
     2041 ggcattttcg ttgcgtttca gctcgataac gacgcgcata ccggatttgt cggattcgtc
     2101 gcggagctcg gaaatgcctt ccagtgtttt ttcccgaacc aaatcgccga ttttctcgac
     2161 cagcttggct ttgttgacct gataggggat ttcgtcgata acgatggctt cgcgttcgcc
     2221 gtttctgcct atgggttcga tatgggtctt accgcgcata acgacgcggc cgcggcctgt
     2281 tttatagcct tcgcgcacgc cgctcaagcc gtagatggtt gccccggtcg ggaagtcggg
     2341 ggcttggata atgtcgatca gttcgtcgat ttcggtgtcg ggtgcatcga gcaggcgcag
     2401 gcaggcattg acggtatcgg aaaggttgtg cggcgggata ttggtcgcca tgccgacggc
     2461 gatgccggac gagccgttga cgagcagtgt ggggaaacgg gtcggcagta caagcggctc
     2521 gtgttcgcta ccgtcgtagt tcgggccgaa attgacggtt tcttcctcaa tgtctgccag
     2581 catttcgtgg gaaattttcg ccatgcggat ttcggtgtag cgcatggctg cggcggcaag
     2641 cccgtccacc gatccgaagt tgccctgtcc gtctatcagc acataacgca tagcgaaatt
     2701 ttgcgccata cggacgatgg tgtcgtatac ggcggtatcg ccgtgggggt ggtatttacc
     2761 gatgacgtcg ccgacaatgc gcgccgattt tttgtaggcg gcattccagt tgtttttcaa
     2821 ttcgtgcatc gcgtacagta cgcggcggtg taccggcttg agaccgtcgc gaacgtccgg
     2881 cagcgcgcgc ccgacaatga cgctcatggc gtagtcgaga tagcttttgc gcatttcgtc
     2941 ttcaaggctt accggcaggg tttcgagggc gaatttgtgg tcgtggcgga tggttgcgtc
     3001 ggtcatggtt tcaatgtttc gtatggcaaa aaattgttgc ttattttagc atattttgac
     3061 gcggaacggt gcggcggtta cgccgtctga aacacggtgc ggattataat gccgaggaaa
     3121 tttcgttgcg gagtttgtcg agaaacctgc cttggcggac ttgttgtgcg gctgtgtcgt
     3181 aatcttgtcg ggcaaacgac tgtttcagac cgcagaacag tttttcttgt tcgtcgcgga
     3241 tttcgttgtc gagatttttc aaggattcaa ggtcgttgcc tgcccgtgcc tccatcagcg
     3301 tttcgcgcca ttccatttgc tgcataagga attcgggggc gaaagcggta tgctccggcg
     3361 cgtcggcatc gatgcccgat gttttcagca ggtaggcggc gcggtcgatg gggtttttca
     3421 aggtgcggta ggcatcgttg atggtggaag acatcatcac tgcctgcttt tgctcaaagg
     3481 cggaagctga agcgaattta tcgggatgga aacgggcggc caaggcgcgg taggtttgtt
     3541 ccaagttttc ggtgtcgata tcgaaagcgg gttcaatccg gaagagggtg aaatattggg
     3601 acatagtagg atgataaatg taagattttg gcagaaaact gtttttgcct tataatctgc
     3661 agcttcttaa acgaaaggac tgaatatggg cggcaaagtg cagcacaata aaggcaaaat
     3721 acgcgacaat gctttaaaag ccttagtgaa atccgatttg ttccggcaca aggtggaacg
     3781 gaaaaggaaa ggcaaaggca gctacaacag gcaggaagtg aaaaaatggc gggacggttt
     3841 tgatacggtc ccgccatttt tatgtcttaa acatggaagc tttcgccgca gccgcaggag
     3901 tctttgacat tggggttttc aaatttgaaa ccttcctgca aaccttcttt ggtgtaatcg
     3961 acttgtgtgc cgtccagata aaccaggctt ttcgggtcga tataaatgcg tgcgccgtgt
     4021 ccttcgaaaa tcaggtcgtc gccatcggct tcatcgacaa attcaaggtt gtacgccatc
     4081 cccgagcagc cgctggtttt cacacccaag cgtacgccca agcctttgcc gcgtttggcg
     4141 agatagtcat tgatgtgttt tgcggcattc tcggtaaggg taatcatatt tcttccttgt
     4201 tgtatcgccc cggacagacc cgaagcggcg ggcggttcgg acggcattgc gggacgatgc
     4261 cgtctgaagg gctttatctg ttttcctgac gtttgcggta gtcggcaacg gccgctttta
     4321 ccgcatcttc agccaagatg gagcagtgga tttttaccgg cggcaattcc aactcttcgg
     4381 cgatttcgct gtttttgatt gccagcgcgt catccaggct tttgccttta acccactcgg
     4441 taatcaggct ggacgaagcg atggcggaac cgcagccgta agttttgaat ttcgcatctt
     4501 cgatgatgcc ttcatcgttc actttgattt gcaggcgcat cacgtcgccg caggccggtg
     4561 cgccgaccat gccggtgccg acagattcgt cgtttttgtc gaaagtaccg acgttgcggg
     4621 gattttcgta gtggtcgatt actttatcgc tgtatgccat gatgtggttt ccttaatgtt
     4681 ttgatgattt aagtggtttg tttgccggtt ttcagacggc ctgagttaga ttttgcacgc
     4741 tccgccttca cagccgtcgt cgttttccga atcgacgccg ccttccacgg taacgccgtc
     4801 aaaatcttca agcaggttgt cgagattgtc caaatctaaa tcttgttcgt tcatattttc
     4861 tcttgattgc tttgtttttg gaatgccgtc taaaggttcg gacggcatgt tggtacgctt
     4921 taatgcgctg cccattcaat cgagttcaaa tcaatcccgt ctttgaacat ttcccacagc
     4981 ggcgacagtt cgcgcagttt gccgattttg gatttaatca gttcggcggc gaattgcact
     5041 tcttcttcgg tggtcatgcg accgaaggtg atacgcaggg atgagtgcgc cagttcgtcg
     5101 ttgcggccga gcgcgcgcag gacgtagctg ggttcgagcg aggcggaggt gcaggcggag
     5161 ccgctggata cggcgagttc tttcaccgcc ataatcaggc tttcgccttc gacgaagttg
     5221 aagctgacgt tcaggttgtt cggggcgcgg tgtccgaggt cgccgttgat gtagacttct
     5281 tcgatgcctt cgatgccttt gaggaagata tcgcgcagtt tcaggtagtg cgcagtgtct
     5341 tgtgccaatt cttctttggc aatgcggaag gcttcgccca taccgacgat ttggtgggtc
     5401 ggcaatgtgc cgctgcggaa gccgcgttcg tggccgccgc cgtgcatttg ggcttcgagg
     5461 cggacgcggg gtttgcggcg gacgtacagc gcgccgatgc ctttggggcc gtacactttg
     5521 tgcgcggaca tcgagagcag gtcgattttg gcggcttcga catcgacagg cactttgccg
     5581 caggcttggg cggcatcgac gtggaagacg attttgcgtt cgcggcagat ttcgccgatg
     5641 gcgggaatgt cttgcaccac gccgatttcg ttgtttgccc acattacgga aacgaggatg
     5701 gtgtcgtcgc ggatggcggc tttgagttct tccaaatcaa tcaaaccgtt ttcttgaacg
     5761 ccgaggtagg ttacttcaaa accttggcgt tcgagttcgc gcattgtatc taaaacggct
     5821 ttatgttcgg ttttgacggt aatgaggtgt ttgcctttgg ttttgtagaa gtttgccgcg
     5881 cctttgatgg cgaggttgtc ggactcggtc gcgccgctgg tgaagacgat ttctttgggg
     5941 tcggcattaa tcagggcagc gatgtcggcg cgggcttttt cgacggcctc ttctgcttcc
     6001 cagccgaatg cgtggctgtt ggaggcgggg ttgccgaagg tttcggtcag atagggaatc
     6061 attttttcgg caacgcgttt gtcaacggtg gtggtggcgg cgtagtcgag gtaaacgggg
     6121 gttttgacgg tcatggtttg ctctttcttt ttcgggtgtt atttaatgga tgtgtgtaaa
     6181 ttggacgacg cggctgccgt cgctgttgtt tttctgttcg atgatgcttt gcagggtaac
     6241 gctgccgagg tagtcgttga tggttttgtt taaattctcc caaaggtcgt gcgtcaggca
     6301 gggcgcaccg tggtggcagt tggctttgct gccgcattgg gtcgcgtcca gccggtcttc
     6361 ggcggcggcg atgatttggg cgatgttgat gcgtgccgcc ggtgcggcga ggatgtagcc
     6421 gccgccgggc ccgcgcaggc tttcaaccag tccggcgcgg cggagtttgc cgaacaattg
     6481 ctcgagatag gagagggata tgttttggcg ttcgctgatg gcactgagtt tgacggcgcc
     6541 ggtttgcgcg ttcatcgcca aatccagcat agcggtaacg gcaaaacgcc ctttggtggt
     6601 cagtctcatg gtggtggttg cccgtgtcgg cttttttata gtggggagaa ttgtccaata
     6661 tctgagtgtt tcagtcaagt aagggggagc tgttgccgat tgtttgttta aatggcggta
     6721 tagtctgatt ttactttatt tgtttggttt tttaaatcag ttggttatgt tttttgagga
     6781 gcggtggtcg gggtattttg ccggcaagcg gtttcaaaac gatttgattt atttttgata
     6841 ttgatataga ttatctaaat cttagtgatt tatttgggta ttgcctgaaa tgctgtctga
     6901 aggctcaagc ggtttcggac ggcatattcc atcaggctgt caaacaggga aaaacaagat
     6961 gaaacgtgat ttgagcaaaa tgacctgcat cgaagacttg cgccgcgttg ccaagcgcaa
     7021 aatgccgcgt atgttttacg attacatcga ttcgggttcg tggacggaaa cgacttatcg
     7081 ggaaaatact tcggatttca aagacatccg cttccgtcaa aaagtattgg tcaatatgga
     7141 gggacgcagc cttgagacta aaatgatcgg gcaggatgtg aaaatgccgg tggcgattgc
     7201 gccgacgggt tttaccggca tggcacacgc agacggcgaa attttggcgg cgcgggcggc
     7261 ggagaagttc ggtattccgt ttacgttgtc caccatgtcc atctgctcga ttgaagacgt
     7321 tgccgagaac accagcgcgc cgttttggtt tcagctttat gtgatgcgcg accgcgaatt
     7381 tatggaaaac ctgattaagc gcgcgaagga tgccaaatgt tcggcattgg ttttgaccgc
     7441 cgatttgcag gttttggggc aacgccacaa agacatcaaa aacggcctgt ccgcgccgcc
     7501 gaaaccgacc atcgccaatt taatcaatct ggcgaccaaa ccggaatggt gcatgaaaat
     7561 gctgaacacg gaacgccgca cgttccgcaa tatcgtcgga cacgcgaaga acgtcggcga
     7621 tttgtcttcg ctgtcttcat ggacttccga acaattcgac ccgcgcctga gctgggacga
     7681 tgtggcgcgt attaaagatt tgtggggcgg caagctgatt atcaaaggca ttatggaacc
     7741 cgaagacgcg gaaaaagcag cgaaaagcgg cgcggacgca ttgattgttt ccaaccacgg
     7801 cggccgacag ctcgacgata ccgtgtctgc catcaaagcc ttgcccgaca tcgtcagcgc
     7861 agtgggcagc gacatcgaag tttggatgga cagcggcatc cgcagcggtc aggatatttt
     7921 gaaggcgtgg gctttgggcg cgaaaggtac aatgataggt cgggcgtttc tgtatggctt
     7981 gggcgcgtat ggagaagaag gcgtaacccg cgcgctggaa atcctatata aggaaatgga
     8041 tatatctatg gcgtttacgg ggcaccgcga tattcaagat gtcgatgcct cgattttaag
     8101 gggtaaagat tggggcaggg aaacggttta gatactgaaa aatacgatgc cgtctgaaca
     8161 cagggttcgg acggcattaa aatttggctg tattttaata atgaactatc tgacaaaacg
     8221 cccttttgta aaggaagttt tgtcaaacgg ggttgcctgc aatgcactac ttcatttttt
     8281 caagcgatgc cgcacctaaa gggaatgact ccgtaaattg tccatttttc tgatgatcag
     8341 taaaaaacgg taaaccgatc aaacgataat gcggtgtatc tttttgcagg atcttatcca
     8401 tcccatattc cacagtgatg gcttctaaaa attcttcttt ccatccatca gtttccacta
     8461 aatgctcacc tttgggctca atgaaaattt ggtaatgcaa aaagtcgttc acaccattgg
     8521 aagaagattt ttgtttatct ttcagcaata aaataaagtc cggcataaat ccttcgccat
     8581 cggcaaagtt attcaattta aacacttctt cattacggat taaatgaacg tcatattgag
     8641 atttcaaatc gcccaaacgc gctgaaataa attgaattaa cgcttcttcc aaacccgttc
     8701 cggcaaaatt attcatcaca taccaatcat tttgcgtggc aatagtcgtt tttacatcat
     8761 cttttttgac ccatttttgt tttggcgtgc cgaaaatttc ccataatttt ttaggtgtaa
     8821 attcttttgt accgataaac ggcatatcac tttcattcag atgtttttca accatttcca
     8881 agattttcag gcagcctgca agtttatcgt ccaggcgaac ctgtttgtct tgccctaatc
     8941 ctaaaaattc aatttgccag tctttcaaca atttattttg caattcattg cgattttgaa
     9001 tattgagttt gctttgcagg cggtcaaaat ggaataaaga ttgactattt ttccctttga
     9061 tatgcagggc tttattgaaa atgtgccgct ccatttcact catttttatg gtttgagtaa
     9121 aattattttg cgtgccaagt tgcttgactt tttcatcatt ttcatcggct gtaaattgcg
     9181 tttcctgcaa cagttgattg ccgtgaactt ggaatgaaag cgtttgcgga ttggcttgca
     9241 ggctgtttgc attattggtt ttggcgtttg ggttggggat ttttttattc gcccaaatta
     9301 acaattctct aaaatcctta ttagcggcaa attcagattt gagtttaaat gttgccaata
     9361 ccttatcatc gtctttttca ggcaaataac cgtcttttcg caactcgttt ttcagttctg
     9421 aaatatagcg ggattgctca tcgtgcgtgt aataaaacaa ttcttccaag atacgcaatt
     9481 cgtgttgcat atcgttgtca aatttgcgtt tattcggctg tttaccttca aacgcaaatg
     9541 gaaaataacg cacgccacga ccaatcaact gcttttccga tacggtggcg gcagccgttt
     9601 tgcctgattt tttatttgaa ccgccgccgt tttgcccttc atacaaacgc acaatatcaa
     9661 acaaattcag aacgtcccaa ccttcggtta atctgtccac cgtaaaaatg gcacgaatcg
     9721 gattatcagc cgcttccaaa ttattcagca atttttctgt ttcgctgtcg gttttttcgg
     9781 ttttggtttt gttggtttcg gagttggtaa taatcacatt gtgtttttga taattttgtt
     9841 ttacccaatc tgccaaatgt gcaaactcaa cgcctttttc ctgcataaat tttagggctt
     9901 gttcagtgcg ggttttgcct tgttcgttgg cgttatcgct atcgctatcg ttcaagcttg
     9961 ttgaaaatgt agttaaaaac gaaaaatcaa ccgcctgcac attttctgcc caatttaaaa
    10021 atgccagata atccgctttt gattcatcaa tcgtcttact tctaaacaac atcacaggct
    10081 tgaaattggc aatgccatat ttcaaagcga tttgatggcg ataccaagca aataataaag
    10141 cgtgcaacac ccgctctttc ttgcccagcg tactggatac caaattgatt tctttggtat
    10201 aacctttttg caaaaattct tttaagccaa attttgtgat gattttatcc gcgtattttt
    10261 gttgcacgtc agcattttca ggcagcgtgg cggtaaattc caacagcaca ttttggctgg
    10321 gattgccatt tttattgagt aacaattcca aaaccatatg ctcccagcct ttgcgttcaa
    10381 tttcggcatt gcctgcattg gcttttaatt ccacttccaa atccaactcg ccttgttttt
    10441 tgcctttggt ttgcgcgttt aaatggtgtg cttcatcgcc cagcatcaca agattcaatt
    10501 cgtgcaaatc cgccaatgtg gtttggtttt cccgttcggt gtgaatatcg ttatacagct
    10561 tttgaatgct ggtaaattta atttcaatgc cgtctgaatg tgggctgaac gtttccactt
    10621 tgcgaatggg aattaccgta tcgccctgca aaatcttctc ggtaaataaa aattttgcgt
    10681 gtgtcgggtc aataaaatta ttttccgttt tatccacgat attgttttga ttcacaaaaa
    10741 acagaaaatg ccgataccct ttttcaaaat aatacaaaat caatgccgcc atcatcatcg
    10801 ttttgcctgc acctgttgcc atattgaaca gcaaatgcgt tggcttattt tttaaatcag
    10861 ggaaatcgtc tagctttgaa gtgcggtcaa aaatcaaaaa gttttcaagg gcagattttt
    10921 gccattcaaa aaaatcatat tttaagtttt tggacgtaaa ttctggcatt tcaagtaatt
    10981 caggcttgta ttttttaacc gctttacgtt cattttcaat cacttcaaac aacgttttat
    11041 tattcgccat cttttttctc cgcttgattt tttactgatt gatagaatgc acggcttaag
    11101 gctttatcat catcgttcag gcaatctgca aattgttcgt catccatttc ggataatgaa
    11161 acatacattt gatttaaatc caacatttca agcaccattt gtttttgttc gtctaatggc
    11221 aaagattgaa attcaggctc ttcaataatt tggctaaatt catttatgct gacgttgtat
    11281 ttcaagaaat agcgttcgca taaaccttca aacagcgttt tgatgccgtc tgaatcttcg
    11341 caagccaaaa tctgttgttt tgcggtttcg ttaaatggag caagttcggc ataaacaaat
    11401 tcgccaccac cttgccaatt cacggctttg gaaataccgc cttgctcgcc atcaatcact
    11461 tttttcaagc gttcaacggc aagcgtttca atataatcca tttgttcaat accgatatat
    11521 tggcggttca ttttgtgggc aacggcggcg gttgtgccac tgcctaaatg gtagtctagg
    11581 acgatgtcgt tttcggatgt ggcaatttga ataagatact gcaataagct ctcaggtttt
    11641 ggagtagtaa aaactttgct gccgaacagt tttttcaatt catctgtcgc atgtgtattt
    11701 aagatatcct gtattaagtt cttataggga gcagaacgtt ctatcggctt gttttcgtta
    11761 tcaaccaaca tatagttttt ataacataca ctccatccag attttttaga ctttgatttt
    11821 ctaaactcca aaaaaccgtt tgttattgcc caatcaattt tatttttact ccatttccat
    11881 atccagccat cattgacaaa ttcagtcctg ccattcggat acgtaaaagt gccatctgga
    11941 cattggattg caaaattcaa actgtcacta tactgcaatc cccctctatc taaattgtcg
    12001 atataatatt tgcctctttc ctgttcaaac ttatcactta atttgtatct ctctgtatca
    12061 taagaaaacg tgtttttatt taatttaact gttttaaagt tctttgagta acataagaca
    12121 aactctgtaa tagtcgctat ctgtttggca tcggacgcac ctgtcttttt ttcccaaata
    12181 aaattgcaga tgaaattttc atttccgaaa acttcatcca ttaaaatttt caaatatgcc
    12241 tgttcgttgt cgtcaattga cacaaaaatc gaaccgtctt ccataagcag ctctttggcg
    12301 atttctagac ggtttttcat aaaagtcagc caagtggaat gattaaattt gtcgttgtat
    12361 ttgaaaccgt cattgcccgt gttatatggc ggatcaatat aaatcaactt cactttgcct
    12421 ttaaactgtt tggcaagcga atgcagggca atcagattat tgcctttgat aataagattt
    12481 tcggccggtg tgccgtctga atggcgtttg atttcgccaa ccgcttgttt gccgtctgcg
    12541 gtatagcgag agaattttga gaatgctttt gcgtcaaaaa gcctatctat ttcatcaaaa
    12601 gcaagtgttt gattaaaaaa gatttcttgt ctttttcggg ttaatttggt gtataattga
    12661 ctgactgact gactgactga ctgactgact gactgactga ctgactgact gactgactga
    12721 ctgactgact gactgactga ctgactgact attattgcgt ttaaaataaa tttcttcgcc
    12781 ttcttcggtg ctttgtccgc catttaacac acagtcttta aacggaaaat ccaacacaat
    12841 atcactgctg tctttcaaaa agcggttgcc gtccgtcaaa ccaatgcggt tggcgtattt
    12901 tgtgtaggaa ttattgatgc tgtgtttatc caagaaaaaa cgaaaatcct gcaatttaaa
    12961 caccagcaca ccattcactt ccacaaagaa atggcgtttt aactcatcat tactcaataa
    13021 caaaccgata atggtcgggt cggttttttc caccaaatcc aacaggatgt ttttggctaa
    13081 aatggttttt tcttcgttcg cccatacttt ttcgtgtggt agtagggctt gggttaattc
    13141 ggtttgaatg tctgtcttca tcgttataaa tcctcatcag ttattttaat gtgccattct
    13201 tgtgtaacgg caaatggctt tgcaaataga ttctaataaa atcaattatt tgatgtcttt
    13261 gctgtttccg ggggcgttcg caaagttgcg ataagcgggg cgggtgtccg agtgtgtcgt
    13321 gattttgcgg aaatatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca
    13381 gtacaaatag tacggcaagg cgaggcaacg ctgtactggt ttaaatttaa tccactatat
    13441 caacacatta ttcaaacaca gcccgtattt ttaaccgccg ttcagacggc ggatgccgcc
    13501 gtgttcatca ggcgcaaggc tttcagacgg catatttctt tttgatattc cgccagaccg
    13561 acaaagcggc cgttttccgt gtaaacgcgt accggcgtgt cggaaggcag gtcttcttcg
    13621 aaacgcggcc gttgcccgca ttggagcata tggacggcat aatcgtttaa aacggtttgg
    13681 ggaaaatggg caaccaatac gtcgcagggc agcagcaggc tgtcgcgttc tgtttcgtcc
    13741 aagtttgcca aggcttcgag cgtgtggctt tgggcgatgg taaagccggc ggtttcggtg
    13801 cggcgcaggg cggtcaggtg ggcgaacgtg ccgatgtgtt tggcgatgtc ttcgctgagg
    13861 gtgcggatat aggtgccttt gctgcaacgc acgtcgatga cggctttggg tgagtcaaac
    13921 tcggcaatat cgatggcgta aacggtaata tcgcgcggct tgcgttcgat gacgatgcct
    13981 ttgcgggcgt attcgtacag cggtttgcct tcgtgcttga gggcggaaaa catcggcggc
    14041 acttggcgga tgttgcctgt cagggcttgg caggccgtct gaaattcggc taaggaaata
    14101 tcggcgcgtg cggcagcgat gatttcgcct tcggcatcac ccgtgctgct ggcttcgccg
    14161 agtttcagcg tggcggtgta ggctttgtcg gcatccagca ggtattgggc gaacttggtc
    14221 gcttcgccga agcagacggg caaaagcccg gttgccaaag ggtcgagcac gccggtatgt
    14281 ccggcttttt cggcacggaa caaacgccgc gctttttgca gcgcggtgtt gctggaaagg
    14341 ccttcgggtt tgtccaagag caaaacaccg ttgacggcac gtttttgggg tttgcctgta
    14401 ttcatggttc gatgttcgga cggcatgggc atatccgtca gtcttcaacc ggtttttccg
    14461 ccgctacttg gtcgataagg gcggacaggt tcaaaccgcg ttccaaggat tcgtcgtatt
    14521 tgaaatgcag ttcggggatt ttgaacagct tgatgcgttt ggccaattcg ctgcggaggt
    14581 gtccgcgcgc gtgttccagc acttcttccg taatttcgcg cgcgtcttgg tttaaaatgg
    14641 tgtagaacac ggtggcgtgg ctgtaatcgc gggtaacttc gacttcgttg acggtaatga
    14701 agccggcgcg cgggtctttc agtccggtac ggacgagttc ggcaagctcg cgcataattt
    14761 gttctttgac acggtcttgg cgggcatagc cgcgttgggg ttttctcatg aatatcctca
    14821 gatttcagat ttggtttcag acggcatttt tgccgccggg gcggttgcga aagggcgtat
    14881 tatagcctaa tcggcgggat tttaaggtaa tacattgata agaacggata tagttattat
    14941 acttggcaac ccgaacggca cgtgccgttt tctgtaacgc atacattgta aagaaagcca
    15001 tgtccaacga aaaccgtacc tgttcctttt gcggaaaatc caaatcacac gtcaaacatt
    15061 taattgaggg cgaaaacgcc tttatctgtg acgaatgcgt ctcaaactgt attgaaatat
    15121 tgcatgaaga cggaaatgac ggcacgcctt cggaaagtgc cggaggggag ccggaagaat
    15181 ccggcaagct gcccacgccc gccgaaatcg tcgccaacct caacgaccac gttatcggtc
    15241 aggaacaagc caagaaagcc ttggcggtgt cggtgtacaa ccattacaaa cgcctgcgcc
    15301 acccgaaagc cggagctaat gtcgaattgt cgaaatccaa catcctgctt atcggcccga
    15361 ccggatcggg taaaaccctg ctggcgcaat ctttggcgcg caaactggat gtgccgttcg
    15421 tgatggcaga tgcaaccacg ctgaccgaag caggttatgt cggcgaagat gtcgaacaga
    15481 ttattaccaa actgttgggc aaatgtgatt tcgatgtcga aaaagcccaa cgcggcatcg
    15541 tctatatcga cgaaatcgat aaaatttcac gcaaaagcga caatccgtcc atcacgcgtg
    15601 acgtgtccgg cgaaggcgtg caacaagcct tgctgaaact gattgaaggt acggtggcca
    15661 gcgttccgcc ccaaggcggc cgcaagcatc cgaatcagga atttatcaac gtcgatacca
    15721 ccaacatcct gtttatctgc ggcggcgcgt ttgcaggctt ggaaaaagtc atccgccagc
    15781 gtactgaaaa aggcggtatc ggtttcggtg cgtccgttca cagcaaggac gaaaatgccg
    15841 acattaccaa gctgttcggc atcgtcgaac cggaagattt aatcaaattc ggtctgattc
    15901 cggaattaat cggacgtttg cctgtgattg caacgttaga agaactggat gaggacgcgc
    15961 tgattaatat tttgaccgag ccgaaaaacg ctttggtcaa gcaatatcaa gccttgttcg
    16021 gtatggaaaa tgtcgagttg gaatttgaag aaggcgcatt gcgttccatt gcgtggcagg
    16081 caatggagcg caaaaccggc gcgcgcggcc tgcgttccat cgtcgaacgc tgcctgctcg
    16141 atacgatgta ccgcctgcct gatttgcaag gcttgaaaaa agtggtggtc ggtaaggcag
    16201 tcatcgaaga gggcaaggaa ccggaattgg tgttcgagtc ctgatggcaa acaaaaccgc
    16261 ttcggaacgt tgaaccacgt ttccgaagcg gttttttgtt tttgatgcct aggggcggtt
    16321 tgcggcggca aattccgtca gcacgtgttc caaacgccgc aaaccttccg ccgtatcctc
    16381 atcgtccaac agcagcgaag gcgcgaaacg caatacgtcc gcacccgcaa ccaaaatcat
    16441 cacgccgtgt ttcaaggcgg cggcggttat ttcggatgcg cgtccgcgat aaggcgcgtc
    16501 caacacgcag ccgagcagca gccccatgcc gcgaacttgc ttaaacacgc ccgtttgttc
    16561 tcccaaagac cgcaatgccg tctgaagttt ctgcccctgg ctgcggacgt tgtgcaaggt
    16621 ttccggcgcg ttgatgatgt cgaaggcgcg gctgccgacc gcacacgcca ttgggttgcc
    16681 gccgaaagtc gagccgtgcg tgccgggttg gaaggcggcg gcaatctttt ctgtcgccag
    16741 catcgcgccg atcggaaagc cgcagcccaa ggcttttgcc gaactcaaaa tatcgggaac
    16801 aacgccgtaa tgttcatagg caaacagcct gcccgtatgc cccatcccgg tttgaacttc
    16861 gtccaaaatc aacaacgcat tgtgccggtc gcacagacgg cgcgcggttt gcaaatattc
    16921 cgcagtggcg ggcaggatgc cgctttcgcc ctgtatcggc tcgatgatga ccgcgcaggt
    16981 ctgttcgccg acggcagctt ccagcgcggc aatatcgttg aacggaacgt gcgtaatgcc
    17041 ttgcggcagc ggtgcataat ccttgctgta tttcggctgg ccgccgacgg acacggtaaa
    17101 cagcgtgcgt ccgtggaaac tgttgataca ggcgacgatt tcgcttttgc ctccgccgaa
    17161 acggtcgcgg gcgtatttcc tcgccagctt caacgccgcc tcattcgctt ccgcgcccga
    17221 gttgcagaaa aaaaccttgt cggcaaaact gtttgcaacc aatttttgcg ccaattcctg
    17281 cgccggacgc gtcgtataga tattggaaat gtgccacagc ttgtgcatct gcgcgtttaa
    17341 agcatcgaca agggcagggt ggcagtgtcc cagcgcattg acggcgatac cgcctgaaaa
    17401 atcaatatat tcacgccctt tcgtatccca aacgcggctg cctgaagcgc gttcgggaat
    17461 catcggggca aaggcgaaat tgggggtcag atagttttgc ataattgctc ctgattgcgg
    17521 ttttgctcgg gattatgcac cgtttttcaa agcagataaa gggaaattgc cgacaatatc
    17581 gggcagcggt aaaaataaac cgaaatattt tcaatcggat agatgaaaaa tcaaaaaaat
    17641 ttcgataaaa tgcttgaggc gcggcagcgt tttgatttag aatctcccca atatttttat
    17701 cttcagacgg catttcatca tgcaaggcaa aaaatttaca accgcttatt gctattggta
    17761 ccacacagac gtgcggtact tttttgcgct tgcttgaaat ccatcccata cgcaaaacag
    17821 gccgctcgaa aacggggcgg cttttttgtt accttcacac aggtcgcccg attcggcaac
    17881 acacgacaga atcaggaaaa tgaccatgaa tgcaatgccc caaaccgccg cacaaaacga
    17941 aaccctgcac acttgtagca ttattgtcga tgatgcacag gatttttccc ccatgcttct
    18001 cgatgcaggg cgggacgaca cactcatcag cgaatcgatg attccgcaaa tccgcactgt
    18061 tgccgccctg atagccgccg aacggcacga cttcagccgc agcagccccg ccgagtttac
    18121 cgatgccgcc gatttttttg ccgcacggat tctcgtattg ggcgtgcgac gcttccatct
    18181 ggacgtatcg ctgcttcaga ttttgaaaac cgccaacagc cgcgcccgcc gctttgccga
    18241 aaaacaccgc ctgtttttca cacccgcaca agccgaattg agcctgaaca aacacaaaaa
    18301 ccgccgcctg ctgaccgtag aaaccgaaca cgaagtcgaa aacaaaggca acccagtcgc
    18361 caacagcctc gcatttgtcc gcaaattgca caccctgccc ctgtaaacat cacgatttgc
    18421 cggcagaatg cttgccaaaa ttcccgacgg cgatacaata aaaccgattt tccacccaaa
    18481 caggacacac atgaaaaaaa tcatcgcctc cgcgcttatc gcaacattcg cactcgccgc
    18541 ctgccaagac gacacgcagg cgcggctcga acagcagcag aaacagattg aagccctgca
    18601 acagcagctc gcacagcagg cagacgatac ggtttaccaa ctgactcccg aagcagtcaa
    18661 agacaccatt cctgccgaag cacaggcaaa cggcaacaac gggcaacccg ttaccggtaa
    18721 agacgggcag cagtatattt acgaccaatc gacaggaagc tggctgctgc aaagcctggt
    18781 cggcgcggcg gcgggcacgt ttatcggcaa cgcgctggca aacaaattca cacgggcagg
    18841 caaccaagac agtcccgtcg cccggcgcgc gcgtgccgcc taccatcagt ccgcacatcc
    18901 caatgcgcgc accagcaggg atttgaacac gcgcagcctc cgtgcaaaac aacaggcggc
    18961 gcaggcgcag cgttaccgcc cgacaacgcg cccgcccgcc aattaccgcc gccccgccat
    19021 gcgcggtttc ggcagaaggc ggtaaatccg gcgtgccaat gccgtctgaa gggctttcag
    19081 acggcatttt tgcatttgtt aggggcattg ttatgttgcc gtttgatttt cagacggcat
    19141 tttgtttcca agcgtttgat gtcgggatgg caattctgat ttacgtgcgt tggcagggtg
    19201 gcattgtcat gccgccgttg ggttttcaga cggcatcata tcgaacagtc aagccgtagg
    19261 gcgagcattc ttgcccaccg ttgcaagggc gggatttggt gggcaggaat gcccaccctg
    19321 catctgaaac ccttccgtgt cattcccgtg caggaaggaa tctagatatt tgaatttacg
    19381 gaaattttta atcttttcca aagcggaaaa gg
//
LOCUS       DAWFJR010000031        19291 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWFJR010000031 DAWFJR010000000
VERSION     DAWFJR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19291
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.031"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(239..2014)
                     /gene="ptsP"
                     /locus_tag="WGR04_001014"
     CDS             complement(239..2014)
                     /gene="ptsP"
                     /locus_tag="WGR04_001014"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ0794585.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIVKVIRCEDE
                     EKSEKLIKQINSVSVEEEADFKGRK"
     gene            complement(2014..2283)
                     /locus_tag="WGR04_001015"
     CDS             complement(2014..2283)
                     /locus_tag="WGR04_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ0794586.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(2352..2789)
                     /locus_tag="WGR04_001016"
     CDS             complement(2352..2789)
                     /locus_tag="WGR04_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ0794587.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(2870..3433)
                     /locus_tag="WGR04_001017"
     CDS             complement(2870..3433)
                     /locus_tag="WGR04_001017"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ0794588.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            complement(3502..4326)
                     /locus_tag="WGR04_001018"
     CDS             complement(3502..4326)
                     /locus_tag="WGR04_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ0794589.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKDQEIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            4417..5049
                     /locus_tag="WGR04_001019"
     CDS             4417..5049
                     /locus_tag="WGR04_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ0794590.1"
                     /translation="MTLRYEILSVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFTLPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(5256..7040)
                     /gene="rmuC"
                     /locus_tag="WGR04_001020"
     CDS             complement(5256..7040)
                     /gene="rmuC"
                     /locus_tag="WGR04_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ0794591.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFAGLNAHLAEKAA
                     RCDFVEQAHGKTVSELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESARLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLADVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQHDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQSSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERSNETTALSESLEYAAEDEAV"
     gene            complement(7378..8166)
                     /locus_tag="WGR04_001021"
     CDS             complement(7378..8166)
                     /locus_tag="WGR04_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ0794592.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            complement(8181..9530)
                     /locus_tag="WGR04_001022"
     CDS             complement(8181..9530)
                     /locus_tag="WGR04_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ0794593.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            complement(9549..10130)
                     /gene="petA"
                     /locus_tag="WGR04_001023"
     CDS             complement(9549..10130)
                     /gene="petA"
                     /locus_tag="WGR04_001023"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ0794594.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(10253..11002)
                     /locus_tag="WGR04_001024"
     CDS             complement(10253..11002)
                     /locus_tag="WGR04_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ0794595.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            11133..12062
                     /locus_tag="WGR04_001025"
     CDS             11133..12062
                     /locus_tag="WGR04_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0794596.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGAPLFERKSTPLRFTPVGERLLRLAHDLLPQVTDAERDLARIIEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPIWPQIELDIVSGFQADPIGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKTVWEAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            complement(12318..12710)
                     /gene="rpsI"
                     /locus_tag="WGR04_001026"
     CDS             complement(12318..12710)
                     /gene="rpsI"
                     /locus_tag="WGR04_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ0794597.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(12720..13151)
                     /gene="rplM"
                     /locus_tag="WGR04_001027"
     CDS             complement(12720..13151)
                     /gene="rplM"
                     /locus_tag="WGR04_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ0794598.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(13329..13508)
                     /gene="ssrS"
                     /locus_tag="WGR04_001028"
     ncRNA           complement(13329..13508)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WGR04_001028"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(13514..13819)
                     /locus_tag="WGR04_001029"
     CDS             complement(13514..13819)
                     /locus_tag="WGR04_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ0794599.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(13830..14159)
                     /locus_tag="WGR04_001030"
     CDS             complement(13830..14159)
                     /locus_tag="WGR04_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794600.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(14214..15203)
                     /locus_tag="WGR04_001031"
     CDS             complement(14214..15203)
                     /locus_tag="WGR04_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ0794601.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(15326..18028)
                     /gene="ppc"
                     /locus_tag="WGR04_001032"
     CDS             complement(15326..18028)
                     /gene="ppc"
                     /locus_tag="WGR04_001032"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ0794602.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRNMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELSHQRPLYSP
                     FITYSDHTRRELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPRSRINIVPLFETIEALENACPVMETMFRLDWYDALLESCGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAAVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            18071..18964
                     /locus_tag="WGR04_001033"
     CDS             18071..18964
                     /locus_tag="WGR04_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ0794603.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDELLLRYSRHILLDEIGIEGQQKLSDAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKLKTEALAGRLKRINHTVNVRTVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGCTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPVCGTAR"
BASE COUNT         4124 a         5254 c         5121 g         4792 t
ORIGIN      
        1 caggcgggaa tccagaacgt aaaatctcaa gaaaccgttt tacccgacaa gtttccgtgc
       61 cgacagacct agattcccgc ctgcgcggga atgacggatt ttaggtttct gattttggtt
      121 ttctgttttt gagggaatgg ccgattttgg gtttctgttt cggttttcta ttttgcaaga
      181 atggcaaaat ttcagattgc gggcattgtt aagtatttct atttttcacc tgccgtattt
      241 atttccgccc tttgaagtcg gcttcttcct cgacagacac gctgttgatc tgtttgatca
      301 gcttttccga cttctcttcg tcttcgcagc ggatgacttt cacaatatcg ctttcgagct
      361 gcccgacatt gctgtgcaga atgatgtttt tgacgggcag gatgttgttg gggttcatgg
      421 aaaaacggcg cagccccata cccaataaaa cgcgggtaaa cgccgtatcg cccgccatct
      481 cgccgcatac ggacacgtct ttgtccatgc ggttggcggt gcggatgacg tgttgcagca
      541 ttttcagcac ggcgggatgt ccgggctggt aaagatggct gacgctgtcg tcgccgcgat
      601 cgacggacaa gatgtattga atcaggtcgt tggtgccgac ggagatgaaa tcgaccagtt
      661 tcaaaatact gccgacggtc agcgcggcag acggaatttc aatcatacag ccgatgccga
      721 ctttaccgaa ggcatcgccg cgttcggcaa gctggcgttg cgcggtgtcg aggtggatga
      781 ggcactggcg cacttcggat acggaggtaa tcatcggcca catcatgcgg acgggaccgt
      841 gtaccgccgc acggaggatg gcgcgcatct gggtgcggaa catgaccggt tcggcaaggc
      901 acaggcggat gccggtcatg cccagcgcgg ggttgaggct gccgttgggc gtgctgtttt
      961 tcccgaacca gcgcgggttt ttgtccacac ccaaatcgac tgtccgtatc gttacgcttt
     1021 tgcctttcat ttttttgaca atcgcgctgt acacttcgta ctgctcgtct tcagacggca
     1081 tcgtatcgcg gttcaggtaa agaaactcgc tgcggaacag cccgatgccg tctgcgccga
     1141 ggttgtgcag cggtttcacg tcttcggcgg attctatatt gcccacaagc tcgatgcaga
     1201 ccccgtcggc ggtggcggcg gcggtttttt tgagcttgtt caaatcgcgt ttgtggctgc
     1261 ggtattcgcg ggcacggcgg cggtattcgt tcaacaccga ctcatccggc gcgataatca
     1321 acacgccgtt gataccgtcc acaatgaccg tttcgccttc ggtaatcagt ttgcgcgcgt
     1381 tgtgcagccc gacgacggac gggatgtcca agctcctgcc caaaatcgcc gtatgcccgg
     1441 tggggccgcc ggcatcggta acgaaggcgg caatgcgctg ctctttaaac aaaaccgtgt
     1501 cggcgggcga aaggtcgttt gcaatcagaa cggtttcgtc aaacaggttg tcggcaactt
     1561 ccaactcgtt gccctgcccg atcaggttgt tgtggatgcg gcggacgact tgcagcatat
     1621 cctgcttgcg ttcgcgcaaa taggcatcgt ccatattgtc gaattgggcg gcgagtttgt
     1681 cgctctgctg cttcaatgcc cactcggcgt tgattttttg ttcccttaaa atatcgacgg
     1741 gttcgcgcga caaggtaaca tcggtcaaga gcatcaggtg cagcgagatg aacgcgccca
     1801 actcggtcgg ggcgttttcg ggaatcgcgc tgcggagctg ttccaactct ttgcgcgtgg
     1861 ctttgacggc ggcatcgaaa cgttcggctt cggcatcggt gtccgcctcc gcaacatcat
     1921 actgcggcac ttcctccgta ccgcgcgcaa tcaggtgggc gcaaccgacg gcaatgcctt
     1981 tgcccgccgc cacgccgtgc agcacgatac tcattattcg ccctcgccga agtagccgtt
     2041 gattaagtcg gtcagggcgc gcatcgcttc cgcctcgtcc gcgccgtccg tctccagttc
     2101 gatgaccgta cccttggcgg cggcgagcat catcagcccc ataatgcttt tgccgttgac
     2161 gcggctgtcg tttttcgtaa cccaaacttc gcttttgaat tgggacgcgg tttgggtgaa
     2221 cttgttggac gcgcgggcgt ggagtccgag tttgttgatg atttcgatgg attgtttgag
     2281 catttcggtt cccgtgttat gtatatcggc agcagacgcc gtttaaaatg ttttcctgcc
     2341 ctgccgcttc ttcagacggc atcgccgctg cgccggcaca ccaaatcttc gggcgcggac
     2401 gtgatggcga aaatgccttt taccgccgcc tccctgacgc attcggtaaa ggcggcaagg
     2461 tcttccgccg ccggcgaata ttggacggcc ttaaccatca tcggcgcgtt cagcccggtc
     2521 aaaatcgccg atttgttttc gcgcacgagg cggcgggcgg cattgcaggg ggtcgcgccg
     2581 aaaatatcgg tcataatcag cacgccgtcg ttgtcgggaa actcctgaag cgcggcaatg
     2641 gcgttgttga tgatgtcgtc ttggtcttcc gtcggttgca cgccgagtat gcggacgttt
     2701 tcaggcagtc cgcccggaaa aaaatgatgt gccagcttgc ggtaggcttc gcctatggtt
     2761 tcgtgtgtga tgattaaaag ccctatcata ttatgcgtcc tgttcctcgt tgtcctaccg
     2821 gcgtatgggc gcgatgccgt ctgaaaggcc ttcagacggc atcgcgtcct tattttccgt
     2881 ccaatgcgta aatctcgccc agattgcgcc agcagcccgc cgcatccatg ccgtaaccga
     2941 aaacataacg gttcggcaca tccagtccga cataatcggc tcggataggc ttgggtttgt
     3001 caatcagctt gttggcgaac accgccgcac ggcagcttgc cgcacccatt tccaaaagtt
     3061 tggcttgaat ggcggacatc gtatgccctt cgtccaaaat atcgtccagc acgacgacgt
     3121 gcctgccccg gatttgttcc gcatcgggca tacgcttcca gttgaacgcg ccgccctcca
     3181 gcttgtcgcc gtaacgggaa acgtgaacat aatcaaaatc taagggaaaa cgcaacagcg
     3241 gcagcaactg ccccgtaaac accaccgcgc cgcccatcac gggcagcagc agcggatatt
     3301 tgccgcccaa atcgcgcgta atctcgtccg ccactttttg cagtgcggca cggcattggc
     3361 cttggtcgaa caaaagatca gcgttttcaa gcatcgcctg tgtttcaagg cgtttggttt
     3421 ctaaatcggt catatgtcgg aatcggtcgg taaaaggaaa attataaacc aaagtatcgg
     3481 atgccgtctg aactgtcctg ctcagcggtc ggtacgcacg cgcacaaatg tggcaaattt
     3541 cggcgtgcct ttccgcgtaa agccacggta acggtaggta atcagcgtgc cgattttggg
     3601 cgggttgtcg cggtctttgt ccttgaaacc gctgccgatg cggaattcgc cgtgtcggtt
     3661 tttgcagccg accgcgccca gccgtccggc gtttcgccct ttgccctcat agtgccgcgt
     3721 taccgtgcat tcgtcgtcgt attggctttt cagcttcaat aattggctgc tcctgccgcc
     3781 gctataacgg gattcgggct ggcgcagcat cacgccttcg ccgccctgcg cttcgatttg
     3841 ttttaaaaag tccatcgcgt gctgccggtc gcgcactttg atttgcggga tgatggtaat
     3901 cggcgcgttc ggatgcgttt tcagccactg cgttgcgact gccaaacgtt ggtagagatt
     3961 gccctgcgcc ttgggtacgt cgaaaacgtg cagacggatg ccgcgccagt ctgaagaaac
     4021 agaacgcacg gtagcggaaa tctgctcgaa ctgaccgcgt ccgctataca attcgccgtc
     4081 caaaggataa ggcggaaact gcgcggtaaa gcctttgggg ggcgtaaacg cgtagccctg
     4141 acggcttatc aggtgctttc cgtcccaata ggcgcgcacg ccgtcgagtt tctcgctcat
     4201 tgcccagcct gcaatctctt ggtctttgta ttcctgcgcc agcatcaaat ccgccgcgcc
     4261 tgctgatgca gggatgaaaa ccgccgtaaa aatcggtatg atgccgccga ttgtcttctt
     4321 aatcatctga ttcccccaat atcaaaacag gcggcaaacc gccataaaac aaacggcaaa
     4381 cccgatgccg tctgaaaaac cgtttaggaa cacgccatga ccctacgtta cgaaatcctc
     4441 tccgttaccc ccttccgcca aaactgcacc ctgatttggg acgacgaaag cggcgaagcc
     4501 gtcctgaccg atgtcggcgg cgacgtgccg ttcctgctgc aagcgttggc aaaccgcaaa
     4561 cttacgctca cggcaatctg gctgacgcac ggccatctcg atcacgcggg cggcgtggtc
     4621 gaaatgttga aaacgcataa agtccctgtc ctcgggccgc atccggacga tgaattcctg
     4681 ctccaatcgc tgccgcaaac caccgcgcaa tacggatttc ccgtctcgcc cgcctttgcg
     4741 ccgaaccgtt ggctcgaaga aggcgaaacg ctcacggtcg gacgctatgc ctttcaagtg
     4801 ctgcatattc cgggccatac gccgggacat atcgtctttt attgtgccga ggcggaattg
     4861 ctgattgcgg gcgacgtgct gttttacgaa accataggca gaaccgattt tccgcgcggc
     4921 aaccacgccg acttaatcaa taatatccgc aacaaattat tcacccttcc cgaaaccgtg
     4981 caagttgtcg ccggacacgg gcgtatgact tccatcggac acgaaaagcg gcacaatccg
     5041 tttttctaac cgccttccct acggtcttca gacggcatca tctgcactga tgccgtctga
     5101 aacacaaaag gctcagacaa ccgccgcctt gccggtgtac ctcgccggac aaggctttgg
     5161 taagtacttc aaacaaaccc aaaatcagaa ccagtacagt tacgccgcgc tttcggcatt
     5221 cccgccccgg ctgaaacaat atttttccgc acaagtcaga ctgcttcatc ttctgccgcg
     5281 tattccaaag attccgacaa cgccgttgtt tcattggaac gctcgaccaa atcccgttga
     5341 agttgtttgc ctgccttcac gcccaacaga cgcagtttct cggcgcgtcc gaccagattc
     5401 ccgcgccctt cggcaagttg cttgaatgcc gtctgaaaac tgctttgcgc ctgatcgatg
     5461 cctttgccga cgctttcgag cgtctgtacg aagccgacaa acttgtcgta cagcttgccg
     5521 ccttcgtccg caatcgccag tgcgttctga ttttgctgtt cgttgcgcca aatattcgcc
     5581 accgtcctca aagtcgccag cagcgtactg gggccgacca gcataatccg tttgtcgaaa
     5641 cactcttgga acaatcccgc gtcatgctgc aacgccagca ggtaggccgg ttcgacaggg
     5701 ataaacataa agacgaaatc caatgtgttc acaccttcca aatcggtgta atccttcagc
     5761 gacaagcctt tcatgtgtgc acggatgctg gcaacgtgtg ccgccagttc gcgtgccgcc
     5821 gtatccgcat ccgccgcctg cgtgtagcgc acataagctg tcagcgagac cttggagtca
     5881 atcacaatct gcttgttgtc gggcaggttg accaaaacgt cgggctggag gcggcgcgtg
     5941 ccgccgtctt cctcttttcg gacggatgcc gcctgaacca catattcgcg ccctttctgg
     6001 aggccggaat tttccaaaac cgtttccaga atcatttcgc cccaattgcc ctgaacctta
     6061 ttctgcgtac cggtcagcgc gttggtcagt gcctttgcct cgctgtgcag ctgcgcattc
     6121 aatccttgaa gccgtttcaa ttcgttttcc aacgtcagcc gctcgcgcga ttctttatca
     6181 taggtttgct tgaccaactc gccgaaaccg tggatgcgtt cgtttagcgg gttcaaaacc
     6241 tgatggagct gctcgcggtt ctgctcggta aaacggcggc ttttttcttc caaaatcgtg
     6301 ttggcaagat tttgaaactg atcgctcaaa cttttgcgcg cctcgcccag taaggacagc
     6361 ttctcttcag aagcaaggcg ttcctgttcg atttgcgttg ccaaacgttc gttttcaacc
     6421 gccaaaccct gtgccttttc ctgcaactcg gtatgcgact gcctcaaccg ctccgcttcc
     6481 gcctcttttt cctgcaaatg ggcaatctgt ttttcggctg cggcaaaacg gttgcccaca
     6541 tcggcaaggt cgttttgcac gtcgcggaca gtttggcggc tttcttccaa atcggtttcg
     6601 attctttggc ggatttggcg ttccaaggca tattggtcgg caaacgcctt ccgttcctgt
     6661 ccgagctgcg ttgccaaacg ttcgttttca accgccaaac cctgtgcctt ttcctgcaac
     6721 tcgatatacg actgcttcag ccgcgccgac tccgcctctt tttcctgcaa atgggcaatc
     6781 tgcttttcgg ctgcggaaaa acggttgccc aaagcataat tttcgtcctg caaatgccgg
     6841 tatttcccgt ccaacaccgc caattccgac acggttttgc cgtgtgcctg ttcgacaaaa
     6901 tcacatcttg ccgccttttc cgccaggtgc gcgttcaaac cggcaaactc gccctgaaac
     6961 ctgcccttca tcagcaacca tgtaaacaac acgcccgaca ccaacgccgc caaaggcagc
     7021 aaaacagtca tcagttccat caatcatcct aatattcaaa cattttcaca ccggacaaaa
     7081 ccgccgctta ttccgattct acctgtttgt tcgacatagc tccaaaaaat atagcggatt
     7141 ggctttaaac ctgttcgaca tcgccttacc atgctgcttg cggtttcaga ccttttccta
     7201 attcaatatc aatctgccac aaaccctgat taagttcccg atgtctgaca tttttagaat
     7261 gatgccgtct gaaatgttgc agctatgttc agacggcata cggattcagg cttttcaaac
     7321 ggcaggcaaa atgaaaaaag gcaaacccta aaggatttgc ccttttgttc caaacgctta
     7381 gtgtacgtct ttccaatatt cttttttcag gaaatacgcc aaaggcagca taaccgcaaa
     7441 taggaaaatc atcacgacat agcctatacg tttgcgttgc agttgtgcag gttcgcccat
     7501 gtacacaagg taattgacca aatcgcgtac atatgcgtcg tactcttttt ggatcacttt
     7561 gccgttaggc agacggcggc tgtgcaaacc ggtagattcc caatacagct taggcttcat
     7621 ctcgccgtgt tcgtctttta ccataaccgg ctgacctttg gcatccaact caacggcttg
     7681 aacgccttgc tgttcccaca acgggtgggg cataccgact ttatcgaata cagtattgtt
     7741 ccagccgctc ggacgggtcg gatctttata gaagccgcgc atataagcgt aaaggtagtc
     7801 tgcacctttg gaacgcgcaa tcaatgtcaa atcgggcgga gcagcaccaa accattttgc
     7861 cgcatctttc gggttcatcg ccgaatgcat gacatcgccg acattatcgg tggtaaacat
     7921 cagatttttc ttgatttctt cgtcagtcaa accgatgtct ttcaaacggt tgaaacgcat
     7981 accgcttgcc gagtggcagg acaggcaata gttggtaaag atttgtgcgc cgcgctgcag
     8041 gctgacttgg tcgcgcaaat cgatatcgac tttctcatag tgtgcatgac cgcccgaagc
     8101 aaatgccgca cccatgggta ctgccaacag caaggcagca aaccaatttt tcatagcttg
     8161 tttcattttg actgccctca tcagatgttg gttgcaaaca ggtaagcacc cacggcggta
     8221 atgccgacgt aaacaaagaa catgattttt tgtttggcgg tgctcatggt tacgcgttcg
     8281 ggaaccggtt tgtttttatc cagcttggta tagaacggca tacccaggaa gaatgcgaaa
     8341 tagacgaagg acaggatgcg cgcgaccaaa gtacgcgtat cggttgccac catcgcgccc
     8401 aaaataccca aaccgataaa ggaaatgatg aacaagacca atgcggtttt gaaaatcgga
     8461 ccgcggtaac ggacggattt cacctcgccc ctatccagcc aaggcagcag ggcaatcaac
     8521 acgaccgccg cgcccatacc gattacaccc caaacctgcg ttcccaagaa cgagggaatc
     8581 gcacgcaaaa tggcgtagaa cggtgtgaaa taccataccg gcgcaatgtg cggaggtgtt
     8641 ttcagcgcat ttgccgcatc gaagttcggc gcttcgagga agtagccgcc gccttccggt
     8701 gcgaagaaca atacggcaca gaagacaatc aggaatacaa ccacacccaa gatgtcttta
     8761 acggtatagt aaggatggaa tgggataccg tcgcgcggga taccgttttc gtctttgttt
     8821 ttcttgattt caacgccgtc agggttgtta gaacccactt cgtgcagggc gatgatgtgc
     8881 gccacaacca agccgagcaa taccagaggt accgcgataa cgtgcagggc aaagaagcgg
     8941 ttcagggtaa cgtcggaaac gttgaagtcg ccgcgaatcc aagtggacaa atccggaccg
     9001 ataacaggga tggcggagaa caggttaata attacctgcg caccccaaaa ggacatctga
     9061 ccccaaggca gcaggtagcc cataaaggct tccgccatca atgccaagaa aatcagagaa
     9121 ccgaaaatcc acaccaactc acggggtttt ttgtacgaac cgtaaatcaa accacggaac
     9181 atgtgcagat aaacaacaat gaagaagaaa gatgcgccgg tggaatgcat gtagcggata
     9241 atccaaccgc cggacacgtc gcgcatgatg tactcgactg cagtaaaggc agcaggcaga
     9301 tggtaggcat tcaggttacc gtccggtttg tagttcatcg tcaggaaaat accgctgacg
     9361 atttgaatca cgaggacaag caaggccaaa gagccgaagt agtaccaaaa attaaagttt
     9421 ttcggtgcgt agtattgagc caaatgctca ttccacattt tagacagggg gaaacgagcg
     9481 tccatccagc ctaacaatgc ttttgctttg ctattggttt ggtttgccat aattatcgtt
     9541 ccttattctt agtcttcgcc caccaagata gttgtgtcgc tcaagtattt atatggcggg
     9601 acaaccaggt tggtcggggc aggaacacct ttatatacgc ggccggccaa gtcgaatttc
     9661 gaaccgtggc aggggcagaa gaagccgcct ttccagtctg cacccaaatc ggcgggggca
     9721 atgtcgggac ggaaggtggg cgagcagccc aaatgggtgc agataccgat ggcgacaagg
     9781 atgttcggct taatcgaacg ggtctcgttt ttagcatact ccggctgctg ttccgcatca
     9841 gaattgggat cggtaagttc gccgttcagg cctttcaggt ctttaagctg ctgatctgta
     9901 cggttgagca cccaaatcgg tttgccttgc cactcggcgg tcagcagctg acccgcttcg
     9961 attttactga catccacctc gacggcagca ccggcggcct tggctttttc cgaagggaaa
    10021 aaactggcca caaacggcgt tgccacaccc aatgctgcca ctccgcccgc gccgcaggtc
    10081 gcgagtgtca ggaaacggcg gcggccgttg ttgatttctt gattatccat tattcagtcg
    10141 tcctaatatt ttgggaatac cgagccatta aacgttgcaa ttttacccag tttgcagtga
    10201 tactcaaagc attatttaaa ataaggtaaa gttttatgat atttctcaag actcaagccg
    10261 gattgttttc gtcaaaatgg cacacttcca acccgaaaac ctctgccgcc gattctgcca
    10321 gcgcgcgtac gccgtaacgt tccgtcgcgt gatgccctgc cgaaatgaaa gccgtacccg
    10381 tttcattggc aaggtggtat tgggcttcag agatttcccc cgtcaaatac agatcgacac
    10441 cttcgtctat tgccgtctga aaaaacccct gcgccccgcc gctgcaccat gcaacccgtc
    10501 ggatttcgcg ttcgggtttg ccgatgacga caggcttacg ttgcaaaact gtttcaatat
    10561 gcgccgccaa tgcgccgagt gtcttggctt gtttcaggct gcccgagttg agcaggtttt
    10621 gttcgccgaa ccgtttttct gtcgcaaaac ccaatctgtc ggcgagttgg gcattgttgc
    10681 ccagtgtggg atgtgcatcc aggggcagat ggtagcctgc catattgatg tcgtgccgta
    10741 acagtgcggc aatccgttct tttttccaac cagtaacggt cggcaactcg tttttccaga
    10801 acataccgtg atgtaccaaa agcaaatctg ccttctgctc cacagcaaaa tcaatcgctg
    10861 ccctgcttgc cgttaccgac gtaacgattt tcccgatata ttccctccct tcaacctgca
    10921 aaccgttagg ggcgtaatct ttaaacaacg ctgtctgcaa tgtttcatta caccaagtca
    10981 gaaaatccct gcgcaatacc atcttttttc ctaatcgctt taaacaagcg ggcattctaa
    11041 tcgcaaaatg tctggaattc acatttttcc gatttgcacc cgcatatgaa ttattttaat
    11101 atgcgccggt tcaatatgcc gtctgaaacc tcatggattc catcatcgaa ctgcgccacc
    11161 tcaagaccct gctggcactt gaagaaaccg gcagcgtctc ccttgccgcc aaacgggttt
    11221 tccttaccca atctgcactt tcccaccaaa tccgtatgct tgaaaaccac tacggcgcac
    11281 cgttgttcga acgaaaatcc acgcccttgc gctttacccc ggttggagaa aggctgttac
    11341 gcctcgctca cgacctgttg cctcaagtta ccgatgcaga acgtgatttg gcacgcatta
    11401 tagaaggaga ggcaggagaa ttgcggattg ccgtcgaatg ccatacctgt ttcgactggc
    11461 tcatgcccgc aatgggcgaa ttccgcccga tatggcccca aatcgaattg gatatcgtat
    11521 cgggattcca agcggaccct atcggactgc tgctgcaaca ccgtgccgac cttgccattg
    11581 tttccgaagc ggaaaaacaa aacggtattt ctttccaacc gctgtttgcc tacgaaatgg
    11641 tcggcatttg cgcaccagac catccgcttg ccgccaaaac tgtctgggaa gccgaagact
    11701 ttatcgggga aaccctgatt acttatcccg ttcccgacga gatgctggat ttgcccaaaa
    11761 aaatcctgat tccgaaaaac atcaacccgc cgcgccgaca cagcgagctg accatcgcca
    11821 ttatccaact ggttgccagc agacgtggca ttgccgccct tccctattgg acagtcatgc
    11881 cctaccttga aaaaggctat gtcgttcacc ggcagattac tgccgacgga ttgcaaagca
    11941 aactgtatgc cgccatccgt accgaagata cggacaagag ctatctgaac aatttttgcc
    12001 aaatcatacg cgaacgcggt tttgcagatt tgcccggact gagcgaactg gaaccggtct
    12061 gaccccttat tcaaccatac ccggcagttt ttctattttt tcatgtatag tggattaaca
    12121 aaaaccagta cagcgttgcc tcgccttgcc atactatttg tactgtctgc ggcttcgtcg
    12181 ccttgtcctg atttttgtta atccactata ctgtttttga tttttgccca atctgtaatc
    12241 tttagattgc caatgggaaa ccgtctacta caaataaaaa aaccctgcga taagcagggt
    12301 tttttgaatt tccaacatta acgtttggag aattgttttg cacggcgtgc tttgcgcaga
    12361 cccggttttt tacgttcgac ttcgcgggca tcgcgggtaa caaaaccggc ttgagacaag
    12421 gcgggtttca acgcggcatc gaagtcgatc agggcacggg taatgccgtg gcggattgcg
    12481 ccggactggc cggtttcgcc gccgccaaca acattgactt tgatgtcgaa agattcggcg
    12541 ttttcagtca gaaccaaggg ttggcgaaca accattcggc tggtttcgcg tgcgaagaat
    12601 tcgtcaacgg gacggccgtt tacgatgatt tgacctgtac ctttaatcag gaatacacga
    12661 gccactgaac ttttgcggcg gcctgtgccg tagtagtatt taccgttcat gtcgcgtcct
    12721 tattttagtt ccaaaacttt gggttgttgc gcagcatggg cgtgttccgc acccgcatac
    12781 actttcagtt ttttaatcat ggcgtaaccc aaaggacctt tgggcagcat accttttaca
    12841 gcttgttcca aagcgcggcc cgggaattgc tcttgcattt cgcggaaagt gcgttcgtag
    12901 ataccgcctg ggaaaccgga atggcggaag tattttttat cttcgaattt ggcaccggtt
    12961 acacgcagtt tgtccgcatt gataacaatg atgtaatcgc cggtatcgac gtggggggtg
    13021 tattcaggtt tgtgtttgcc acgcagacgg ctggcgactt cggccgcaac gcgacccaag
    13081 actttgtctt gggcatcgat gacgaaccat tcgcgcttca cctcgtgggg tttcgctgaa
    13141 aaggttttca tagtggaaat ccaaatagat atagaaagtt gtaaatttta aagacaggat
    13201 tcgattttgt caatcgcatt accgcgttac ggaaggattt tccggatttc ggcagactgc
    13261 atactgcttt ttcgggcggg gcggcggcca atgtgaaaaa ccgcatcgtt gcgatgcggt
    13321 tttgaatggg aatccccgcg agagccattt cggccgaatc cgcttgaacc ttgctgacaa
    13381 ggcggctgcc tcgggtagtt tcgggtgcgt ccgcaaaagg acgctcgcgc ccactactgc
    13441 tcccggcaac cttaagcgaa cttattggtt caaaggaata tatgccttcg cggacaccgc
    13501 agggaaaaag gggttattcc tgcgccaagc gggatagtgc tttttggcag gcgttgtcca
    13561 tatcggctat tttacgcgca aaatcgccga ttgccaaatc gccgccgttc agggaggttt
    13621 tcaacaggtc gtggacgacg ttgagcgcgg ccataatgac gattttttcg ctgtccgcga
    13681 cgcgtccgcc ttcgcggatg gcttcggctt tgccgttgag cattccgact gcctgcaaca
    13741 gtgtgtcttt ttcttctgcc ggcgtgttga cggtcagccg ggcgtgcatg acttcgatgt
    13801 ggacttgttc gatgttcatc ctttaatcct tattgctgcg tttcctgcca ttgggggagg
    13861 cgcgctgcca gtgcgctgat tttttccctg ctctgttcga gcaggctgcg gtatcgtgta
    13921 ttttcttctg tcaggctgtc gattttgttt tgcaggtctt ctttgagttt gccgacttgg
    13981 acgagcaggg cttcgctgag ttcgtcgacg gcggtttcgt gttcgagttt ttgccgctcg
    14041 tgcgcccgtt tgagttcggc gacggtttct ttgaggcggc ggttttcgct gacgagggtt
    14101 tcgaattttt gtaccagcgt ataaacgctg ctttcgagtt tttcgatatt ttgtttcata
    14161 accttacctg tccgtatgcc gtctgaaggc ttcagacggc atctgtctgt tgtttattca
    14221 aaacgcgcgc tgcgttccat cagcctttcg acgacctgtt gcggggtcat ttctttgcgg
    14281 atgagttgca gcagggtttg ggtaatcggc atgtcgattt ggtacttaca ggcagtattg
    14341 aagacttctt ctatcgtgct gaccccttcg gaaacgtgtc cgatttcgac cagcacctga
    14401 tgcagttcct tgccttctgc caaacccaag ccgacgcggc ggttgcgtga gagtgcgccg
    14461 gtgcaggtca agatgaggtc gccgatgcct gccagcccca tcatggtttt gggctgtgcg
    14521 cccattgcgg aggcaaggcg ggtgatttca gctaatccgc gcgtaaccag tgcggcgcgg
    14581 gcgttaagcc cgtactctag accgtcggac aatccggtgg caatcgccat aacatttttt
    14641 accgcgccgc caaccgccac gccgataacg tcggtactgc cgtaaagcct catgacagtc
    14701 gtgttgagct gcggtacgag ttcttcaatc cactcttggt tttcggaggc gaggacgacg
    14761 gcacagggca gttgtttggc gagttcctgt gcaaaactcg ggccggagag tacgccgatt
    14821 ttcttattgt cgggcaatac ttctttcaat acttgaaagg tcagcagccc tgtatcctgc
    14881 tcgaatcctt tgcaggcggc gaggacgggg aggtgtcccg cgccgtactg tttgagcagc
    14941 tctgcgctgc ttctcaatcc ggcaacggag gttacgataa ggacaagtcc gctgtctttg
    15001 agcgcgtctg ccaaatccgc acacacttcc aaggtttcgg gaaaggaaaa gccgggcagt
    15061 ccgcgtttgt tttcacgcgc ttcctgcatt tggcggactt ggtctgcgtt gcgcgtccac
    15121 agggatacgc ggttgccgtg ttgggaaaag tgcagggcga gcgccgtacc ccacgaacct
    15181 gcgccgataa cggtaatttt cattggtcgt ctttcaacat atcactgccg ttcactttaa
    15241 aacaatcggt gtttctctgc aagtgcggtc agggaaatgc cgtctgaaag gtgttcagac
    15301 ggcattttgc cccgatgcgg cacaatcagc ctgtattgcg caaaccttgc gccacgccgt
    15361 tgatggtcag gtgaaccatc agcagcgcgt gcggattgtc gggttcttta cgcaggcgtt
    15421 tgagcatggc gacctgcagg ccgttgagcg cgttcaggta tggaatcctc aaggcgagcg
    15481 aacgggcgag gctgcggttg tcgcgtaaaa gttcttcggt ttgcagcagg tcgagcaggg
    15541 ctttgcggct gcgttggtat tcttccttaa tcatgccgaa gatgacggcg gctttttccg
    15601 gcgattcgct caagccggca tagttttccg cgagggtgat gtcggttttc gccatcactt
    15661 gctccatatt ggaaagcatg gcttggaaga acgggttgct ttgggcgtgt tcgcgcaggg
    15721 cggcgagcgt ttcgggtttg tcttcgcaca aggtttccac cgcgctgccg aaaccgtacc
    15781 aagccggcag catgaggcgg ttttgcatcc atgagaatac ccacggaata gcgcgcaagt
    15841 cctgaatccg cgccaaggtt ttgcggctgg caggacggct gccgaggttg agggtggcga
    15901 tttcctgaat cgggctggtt tgcagaaagt agtcgatgaa gtcggggtgg gtaatcagtt
    15961 cgcggtagta tttgaacgat acgtccgaca atgcctgcat cagtttggca tcagggtctt
    16021 ttttatccgg caggatgctg gcttccaaag tcgcggcaac caaggtttcc aagttgcgtt
    16081 gggcattgcc ggggtcggcg tatttggcgg taatgacttc gccttgttcg gtaatgcgga
    16141 tttgtcccgc cacgctgccc gccggttggg cgagaatggc ttggtaagaa gggccgccgc
    16201 cgcgtccgac gctgccgccg cgaccgtgga acaggcgcat gcggacatcg tattttttga
    16261 agagttcgac caagcccaat tccgcctgat acagacacca tgagctggta acatagccgc
    16321 cgtctttgtt ggagtcggag tagccgagca taatttcttg gatgttgccg cagctttcga
    16381 gcagggcatc gtaccaatcg aggcggaaca tggtttccat gaccggacag gcgttttcga
    16441 gtgcttcgat ggtttcaaac agcggcacga tattgatgcg gctgcgcggt ttgccgtttt
    16501 ccaccgccag caggccgctt tctttcagca gcaatgccaa ggcgagcagg tcgctgggtt
    16561 gttcgcagtt ggaaataatg ctttgtgtaa cggcatcttc gccgaattcg tctttgattt
    16621 tgcgcgcttc gttgaaaatc gccagttcgc ggcgggtatg gtcgctgtat gtgatgaacg
    16681 ggctgtacag cggacgttga tggctcaatt cgcgcaacag ggcggcttgt ttttgctctt
    16741 cgttcaggct gttgtagtct tccaagcctg cgtgttggaa aagctcggca accacatcgg
    16801 cgtgtttgcc tgcgtgttgg cgcaggtcga gcggcatcat gtggaagccg aacacggaca
    16861 cggaacggat gaggtctgcc aaacggcctt cagcaagcag gcggctgccg ttgtcgataa
    16921 gggaacgttg caattttttc aaatcatcga gaaattcttg tgccgaagca tacggctcga
    16981 gaaagccgaa tttgcagccc atgcccaaac cgagcgcgcg cgctttgccc atagcgcgcg
    17041 ccatgatgta agcaatggcg cggcggtagg gttcttcggt gcgagcgatt tcttcgtcag
    17101 gcgagagggc tgccagtgcc attacgtcgt cgttgacttt gacgcggcgg atggagagcg
    17161 gcagttcgcg gtaaagtttg tcgagttcgc cacggtaaaa acggaacacg gcatcggcgt
    17221 ggcggcggaa ggcaaaacgc aaggtttcgg cagaaacgaa cggattgccg tcgcggtcgc
    17281 caccgatcca gccgccgatt ttgaggatgt tcggaacgca gacatcggga tagaccatct
    17341 gaaagtcgtg ttccatattg cggtagagct tgggcagggc ttcgaaaaag ctcatcggga
    17401 agatggacac gccgttgttg atttcgtcgt tgacgctgag tttgtggcgg cgcgtttcgc
    17461 tggtctgcca caagcccagc aggatagtgt cgatttcgcg gcgcagccgt gccagcgcgt
    17521 cggcattggt gcagcgttcg cgttgcggca acagtgcgcg gatgcggcgg ttgaagctta
    17581 agacggtttg gcgttgcact tcggtcgggt gcgcggtcaa aacggcggta acggacgtat
    17641 tgtccaactg ccgctgcacc gatttgccgt cggctttccc cgctttgagc ctgcggacgg
    17701 tttccgtcag gctgccttcc gcgccgccgc gtccggcttc ttcgtggatt tggcggcggc
    17761 gttcgtggtg cacgtcttcg gcgatgttca aaatctgggc gaacaggccg caggccaagg
    17821 ttaaatcgtg ggtttgttgt tcgtccaatt gcggcaatac tttttcaatc aatgccgcgc
    17881 tgtcgtcgga agtggacaag agtttgaccg tttcgacaac caacggcgag gcttcttcgt
    17941 gcaggaggtt gaacagggat tgtttcagaa attccgcgtc cgccgccaaa gccgcgtcct
    18001 ttggattgtt cagaatatgc agttgcatga tttttctctc attgccgtaa atactgtaaa
    18061 tgtacctcaa atgccgcatc cgtgccaaac cgttcacact ttaaccgccc gtgtcccgaa
    18121 atgccgtctg aagttgaacg ccgcccgacg gcagcgttac aatcgcccgc aactgttttt
    18181 tccgaacatc atcatgacca cgaccgaaca cgacaacgac gacgaactcc tgctgcggta
    18241 cagccgccac atcctcttgg acgaaatcgg catcgaaggg cagcaaaagc tttccgacgc
    18301 acatattttg gtcgtcggat gcggcggttt gggtgccgcc gcactgccct accttgccgc
    18361 ttcgggtgtc ggcacgctga ccatagccga ttccgacacg gtcgaactgc acaacctgca
    18421 acgccaagtc gcatttgacg agggcgatgt cggcaaactc aaaaccgaag ccttggcagg
    18481 ccgcctgaaa cgcatcaacc ataccgtcaa cgtccgcacc gtcaacgaaa aacttgacgg
    18541 ctgccgcctg accggtttgg ttcaaaccgc cgacatcgtt ttagactgct gcgacaacta
    18601 cgccacgcgg caagccgtca accgtgcctg cgtgcaaacg aaaacaccgc tggtttcagg
    18661 ggcggcggta cgctttgaag gacaacttgc cgtgtaccgt cccgacttgc ccgactcgcc
    18721 gtgttacgcc tgcctgtttg acggcggatc ggcttcagac ggcatctgtt ccctcttcgg
    18781 cgtgttctcg ccgctggtcg gcatcatcgg ctgcacgcag gcggcggagg ctctgaaaat
    18841 cctgctggat gcgggcgaac cgtcgcacgg caggctggcg gtttaccgtg ccttggaagg
    18901 gggctggcaa tatttcgacc tgccgcgcaa ccctgaatgc ccggtttgcg gcacggcgcg
    18961 ataaaccctg ccgccgtttc agacggcatc caaacggatg cggcggaacg gttttaaaaa
    19021 tttaaaaatt tacatttctt tgcaaaaaaa aataaactta ccttataatt gcaattgttt
    19081 tagcaatgtc tgtttcgcag actcattgag taaaacgttt tccccgtaat gtgtttggcc
    19141 gtctgtcccc tttgggttcg gacggctttt ttttggctgt gtttgaatac ccggttggtt
    19201 ttatctgttt gcagcggggg aagccgctta tttccgttcg ggcggaaaac ggttccatcg
    19261 gataaaaggc attttgtccg actgattaaa g
//
LOCUS       DAWFJR010000032        18745 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWFJR010000032 DAWFJR010000000
VERSION     DAWFJR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18745
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.032"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            142..571
                     /locus_tag="WGR04_001034"
                     /pseudo
     CDS             142..571
                     /locus_tag="WGR04_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
     gene            complement(619..1962)
                     /locus_tag="WGR04_001035"
     CDS             complement(619..1962)
                     /locus_tag="WGR04_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ0794604.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDDIPLAGAWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1967..2620)
                     /locus_tag="WGR04_001036"
     CDS             complement(1967..2620)
                     /locus_tag="WGR04_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ0794605.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2771..3061
                     /gene="gatC"
                     /locus_tag="WGR04_001037"
     CDS             2771..3061
                     /gene="gatC"
                     /locus_tag="WGR04_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ0794606.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAVAPEVRNRLYIVPQVIEE"
     gene            3124..4569
                     /gene="gatA"
                     /locus_tag="WGR04_001038"
     CDS             3124..4569
                     /gene="gatA"
                     /locus_tag="WGR04_001038"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ0794607.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGIPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPSAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGSSGGGLPIGV
                     QLIGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4566..5498
                     /locus_tag="WGR04_001039"
     CDS             4566..5498
                     /locus_tag="WGR04_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ0794608.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLTDIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5541..6971
                     /gene="gatB"
                     /locus_tag="WGR04_001040"
     CDS             5541..6971
                     /gene="gatB"
                     /locus_tag="WGR04_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ0794609.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINQKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKGQEEFGTRREIKNLNS
                     FRFLEQAINYEVEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAHDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPKLPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ASGQGKPTANWMNGELAAALNKAGLELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWEEPEATIAEIIEKHGLQQMTDTGAIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7078..8088
                     /locus_tag="WGR04_001041"
     CDS             7078..8088
                     /locus_tag="WGR04_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ0794610.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMDGHSEQALSEAEKAQGKILMCCTTAQSDISINIPGYKADALPVRTLP
                     ARIESIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDNGKPVILLATGTGYAP
                     IRSILLDLIRQNSSRVVHFYWGARHQDDLYALEEAQGFACRLKNACFTPVLSRPRECW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8218..8850)
                     /gene="pdxH"
                     /locus_tag="WGR04_001042"
     CDS             complement(8218..8850)
                     /gene="pdxH"
                     /locus_tag="WGR04_001042"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ0794611.1"
                     /translation="MDLHNIREDYSKRELSEADCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLLEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            8839..9066
                     /locus_tag="WGR04_001043"
     CDS             8839..9066
                     /locus_tag="WGR04_001043"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794612.1"
                     /translation="MQIHLLLLLSFERFYSRTQTDFNRRTNFAPTPSRSDDFIRKTAA
                     SGTISNRPTEDGILSTRPAAHAAEEPPYQAS"
     gene            9075..10124
                     /locus_tag="WGR04_001044"
     CDS             9075..10124
                     /locus_tag="WGR04_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0794613.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETGKTASQPY
                     GQKASDGIKPQNVPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWITNGWITVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRIGFGPIGLPNRLKRGQFYELNPAEVANIIK
                     WADMLLPGERRRKKA"
     gene            complement(10193..11734)
                     /locus_tag="WGR04_001045"
     CDS             complement(10193..11734)
                     /locus_tag="WGR04_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ0794614.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAIKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSVGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMACLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(12345..13700)
                     /locus_tag="WGR04_001046"
     CDS             complement(12345..13700)
                     /locus_tag="WGR04_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ0794615.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVAGKISIYETRGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPARPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGTGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKDTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            13897..14718
                     /gene="nadE"
                     /locus_tag="WGR04_001047"
     CDS             13897..14718
                     /gene="nadE"
                     /locus_tag="WGR04_001047"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ0794616.1"
                     /translation="MMRIFSTGGKMDTQAVITHIARWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRPTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGIHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(14786..15259)
                     /locus_tag="WGR04_001048"
     CDS             complement(14786..15259)
                     /locus_tag="WGR04_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0794617.1"
                     /translation="MTDFSVWETAPFGATVDYILQRYHNVHRAQFEELVPLAQKVVQV
                     HADTFPAEIAGLLADMQNELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTDNFQPPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            15557..15889
                     /gene="trxA"
                     /locus_tag="WGR04_001049"
     CDS             15557..15889
                     /gene="trxA"
                     /locus_tag="WGR04_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ0794618.1"
                     /translation="MSSELIIHTSDAAFEKDVLNSDTPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(15942..16889)
                     /locus_tag="WGR04_001050"
     CDS             complement(15942..16889)
                     /locus_tag="WGR04_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0794619.1"
                     /translation="MTDITPFANRLGKNIKHLMKWVKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWAKRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMLDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            17217..18605
                     /locus_tag="WGR04_001051"
     CDS             17217..18605
                     /locus_tag="WGR04_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0794620.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQKLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKPAGGKPKG
                     KGGWGDRKAGGRRGDHKPGKEGFGGKTRGEGFKKESFKKDGFKKTGEGFKGKRKAGDS
                     FAGRGERRYKDR"
BASE COUNT         4686 a         5472 c         4750 g         3837 t
ORIGIN      
        1 ttttgggaat ctgttttacc ccaatatata aagcaccata ttaaggcgga gtgtcttccc
       61 cactttgacc cgaacccgga aaagacaccg cccaagccaa tcctgatgct gccccgacag
      121 ccaaccatta aggaaatcct aatgaacttt gctttatccg tcattatgtt gaccctcgcc
      181 tctttcctgc ccgtcccgcc tgccggagcc gccgtcttta cttggaagga cggcggcggc
      241 aacagctatt cggatgtacc gaaacagctt catcccgacc aaagccaaat cttaaacctg
      301 cggacgcgcc aaaccaaacc ggcggtcaaa cccgcccaag ccgacgcagg gaagcgcaca
      361 gacggcgcgg cacaggaaaa caatcccgac actgccgaga aaaaccggca gcttgaggaa
      421 gaaaagaatt gccgaaaccg aacggcagaa caaagaagaa aactgccgga tttcaaaaat
      481 gaacctgaag gcggtgggaa actcaaatgc gaaaaacaag gacgatttga ttcggaaata
      541 caataacgcc gtaaacaaat actgccgtta atcggctcta gcgcaaaccc gatgccgtct
      601 gaagcggcac ggggtttgtc atttctgcca gtaggttttg acgttgacga actcgtacag
      661 cccgaattcg gacaattcgc gcccgtaacc ggaatctttg actccgccga aaggcaggcg
      721 caaatcgctg ctggtatggc ggttgataaa caccgatccc gcctgtattt tttcggcaaa
      781 ccgccaagcg cgttcggtat cggcggtata aatgcaggca cctagcccga acggggaatc
      841 attggcaagg gcgatggcat gttcttcgtt ttcggcgcgc aaaatcaggg cggccggccc
      901 gaatacttct tctctccaga cgcggcaggc aggatttacc ctgtctaaaa ccgtcgcggg
      961 ataaaaccag cctcgccctt gtgggatttt tccgccggtc aggcataccg cgccgtttga
     1021 aacggcatct tcaacctgcc cgtgaaccct gtcccgcaaa tcttcgcggt gcagcggtgc
     1081 aagcgtagta tcgggatgtt tggggtcgcc cattttcaat ttggcgcatt cggcaagaaa
     1141 cagcgtgata aaacgatcgg ctgcggcttc ggttacgatg atgcgtttgg cggcgttgca
     1201 cgattgcccc gcatcgcgga aacgggaata acaggcttct gcggcggcgc gttccaaatc
     1261 ggcatcgggc atcacgataa aggcgttgct gccgccgagc tccaacacgg ttttcttaag
     1321 gtttgcgccc gcgtgtgccg caaggatgcg ccccgtatgc gtcgagccgg taaacgccat
     1381 cgcatcggta tcttcaaccg ccttgagcgt gcccgcctca tccaaccacg cgcctgccaa
     1441 aggaatgtcg tctgaagcca aatcgaacaa cgcctgactg acgcgtgcca cgctgggcgc
     1501 gggtttgacg gcgcacgcgt tgcccgcgca catagcggga acggcgaaac gcaatacctg
     1561 ccagacggga tagttccaag gcatgacggc aaacaccacg cccaaaggct cgaagcgcac
     1621 ctgactcaaa ctcgcctgcg tcgcgatggt tttgtgggca agcagttcgg gggcaaggcg
     1681 ggcgtaatag cgtatcagct cgatagactt gccgatttcc gcgcggcatt cgtgcaggca
     1741 gcgtccgact tcttcgcaca ccatttccgc aaaacgctct ttctccgcct ccaaacggtc
     1801 ggcaaatttt tgcaggcgcg cggcacgttc ggttacgccc agttgcgcga acgccccgcc
     1861 gcgcattttc aaatccgcca gccgccgttc aaactccgca taatcttgag cggggcggcg
     1921 gtaaagcgtt tcgcccgtaa atacattgac actgtgaaac atccaatcaa cctgccagtt
     1981 gcgggaatat cgttttcagt cccgacacaa taatctccac cgataccgcc gccagcatca
     2041 tacccataat gcggtttaaa atcgtcagcc ccgtcgcgcc cagcaggcgg ctgaccttcc
     2101 cggcaacgat taaaatggca taacaaatcg cactgaccac caaaccggcc gcgataatca
     2161 acgcgatgtc gccgtatgtt ttagccgccg aagcgtaaat aatcacggtc gaaataccgc
     2221 ccgggccgat ggtgatcggt atggcgatgg gcacgacggc aatcgctccg gcattgcggg
     2281 cggggcgcgc ctgccccgtt tccggctgcg cgccgagatt ctgcttggcg ggattgtcgt
     2341 tgccgttcat catagaaatg gcaatcagca acaccaaaat tccgccgccg acctgaaacg
     2401 aaccgacgct gatgcccaaa accttcagca gcgtaccgcc gatcagcgca aataccgcaa
     2461 tcacggcaaa cacggcaacg gcggccgtcc gcgcgacctt cctgcgctcc ttcgtgctgt
     2521 gcccgttggt caggtcaagg taaagcgaca acgcgctaaa cggattaatc agcaccaaaa
     2581 aagccacaat cagcttgccg atttccatgc ccaatcccat tatttccccc tccttcaaac
     2641 ccgtgcggca ggcatccgat gctgcaaatt gccgccgcaa cggatttttc cgttataatt
     2701 aaaaattcaa gcaatacgcc ccatcatacc cgaacgacgg tatctttacc atcagacaag
     2761 gatgcttttc atggcactga cacttgccga cgtagacaaa atcgcccgac tctcccgact
     2821 gcacctgact gcggaagaaa aagaaaaatc gcttcaagaa ttaaacgaca ttttcactat
     2881 ggtcgaacag atgcaaacca tcaacacaga cggcatcgaa ccgatggcgc atccgcacga
     2941 ggccgccctg cgcctgcgcg aagacgaagt aaccgaaacc gaccgcgccg ccgaatatca
     3001 ggcggtcgct ccggaagtac gcaaccgtct gtacatcgta ccgcaagtta tcgaagaata
     3061 atccgaatat gcttcagacg gcatcagcaa taccgcccga agccctttaa ggatggaaga
     3121 tttatgaccc aatacacatt aaaacaggca agcgtcctgt tgcagtccaa acagatttcc
     3181 gccgtcgaac tggcaagcgc ataccttgcc gccattgccg aaaaaaatcc cgccctcaac
     3241 ggctacatca ccatcgacca agataaaacc ctcgcagaag cccgtgccgc cgacgaacgc
     3301 atcgctcagg gcaacgcttc ggcacttacc ggcatacccg tcgcctacaa ggatattttc
     3361 tgccaaaccg gctggcgcag cgcgtgcgct tccaaaatgc tcgacaactt catctccccc
     3421 tacaccgcca ccgtcgtcca aaacctgctc gacgaaggta tggtaacgct cggccgcacc
     3481 aatatggatg agttcgctat gggttcgacc aatgaaaact cattctacgg tgcagccaaa
     3541 aacccatgga atcttgagca cgtacccggc ggttcgtcag gcggttccgc cgccgtcgtt
     3601 gccgcgcgcc tcgcccctgc cgcgctcggt tcggacaccg gcggctctat ccgccaaccc
     3661 gcatcgcact gcggtatcac cggtatcaaa cctacctacg gcacggtttc ccgcttcggt
     3721 atggtcgcct acgcctccag cttcgatcaa gccggtccga tggcgcaaac cgccgaagac
     3781 tgcgcgattc tgttaaacgc gatggcaggt ttcgacccca aagactccac cagcctcgaa
     3841 cgcgaaaaag aagactacac ccgcgatttg aaccaaccgc tcaaaggttt gaaaatcggc
     3901 ctgcccaaag aatatttcgg cgaaggcaac agcgccgatg ttctgacggc attgcaaaac
     3961 accattgatt tgctgaaagc ccaaggcgcg gaattgattg aagtttccct gccgcaaacc
     4021 aagctgtcca tccccgccta ctacgtcctc gcctccgcag aagccagcac caacctttca
     4081 cgttacgacg gcgtacgtta cggacaccgt gccgcccaat tcgccgattt ggaagaaatg
     4141 tacggcaaaa cccgcgccga aggtttcggc agcgaagtca aacgccgcat catgatcggc
     4201 acttatgtac tgtcgcacgg ctactacgat gcctactatc tcaaagccca aaaactgcgc
     4261 cgcctcgttg ccgatgattt tcagacggca tttgcacggt gcgacctcat cctcgcgccg
     4321 accgcaccct ccgccgcacc caaaatcgga gcggatgctt cgccggttga aacctacttg
     4381 agcgatatct acaccatcgc cgtcaacctc gccggactgc ccgcattgac cctgcccgca
     4441 ggctccagcg gcggcggact gcccatcggt gtacagctta tcggcaacta cttcgccgaa
     4501 gccaaaatcc tcggtgcggc gcatcaaatc caactcaaca gcgattggca cggcaaacga
     4561 cccgaatgaa gcagaaccga acctttacct tccccgattt tcgcaccgtt tacagctatg
     4621 cgcctttata tcggctgcaa catttaaaat acacattgcg aaaatttttc ggaaaaaaag
     4681 aaatttacgc cttcgagcag tttgtcaacg cctcccctat ccgtcagggg ctgttcctcc
     4741 actgcccgca aaatgcctat ccgctactgc gcgaatttgt tgacaggcgt tttaactgca
     4801 aacgccgttt agatgcgatg acggcagatt ttctcatggc ggaaaaactg ttcggcacag
     4861 acatcctgca ccaaatggaa gactaccgct tccatttggt cttggcgcac ctttcagacg
     4921 gcatcagctt gtggctcaac cgcaacgaca actgcgtcga agaaggcgcg tggtctttat
     4981 ctttgcgcga cgaagcaggc aaccggctgt atatggcgac cttcgccttt gtcggcacac
     5041 acctgctgac agcctccgta caagggccgg cgggtgaaga agccaaagac accgtccgcc
     5101 gcataaccaa acaactccac ggcttgcgtc cccaacaact gatggtaacc gccctgcaat
     5161 atttcgccgc cgtactcggc ttggacggcg caatgggcat tgcacaaaaa catcaggtca
     5221 aactgcgctg gaaacttaaa aagcgtgtca aaatgaatta cgacgcattc tggcaggaat
     5281 acggcgcaag tttggaacgg gacggctact ggcatctccc ccaaaccccc gcccgcaaag
     5341 accttaccga catcgaaagc aaaaaacgct cgatgtaccg caagcgttat gaaatgctgg
     5401 acaacatggt tgcagagatg aaggacggtc tgaaaacaga agcacgcggc atttcagacg
     5461 gcatccaaac ggaaaaaccg ctccgccgga cagcctgacg cgaagactat cgaattgata
     5521 ttttagagaa agaagctctt atgacctggg aaaccgtaat cggcttggaa atccacgtcc
     5581 aattgaacac caaatccaaa atcttcagcg gcgcatcgac cgcattcggc gcagaaccca
     5641 acgcgcacgc cagcgtggtg gaatgcgcgc tgccgggcgt actgccggta atgaaccgtg
     5701 aagtcgttga aaaagccatc aaattgggtt tggctttaga tgcgaaaatc aatcagaaaa
     5761 acgtgttcga ccgcaaaaac tacttctatc ccgacttgcc caaaggctat caaatcagcc
     5821 agttggactt accgattgtc gaacacggca aattggaaat cgtggtcggc gacgatgtga
     5881 aaaccatcaa cgtaacccgc gcccatatgg aagaagacgc gggcaaatcc gtgcatgaag
     5941 gcttaaacgg cgcgaccggt atcgacctga accgcgcagg cacaccgctg ttggaagtcg
     6001 tatccgaacc cgaaatgcgc tccgccgccg aagccgttgc ctacgccaaa gccctgcaca
     6061 gtttggtaac ctggctggac atttgcgacg gcaatatggc ggaaggttcg ttccgcgtcg
     6121 atgccaacgt atccgtgcgc ccgaaaggtc aagaagagtt cggcacgcgc cgcgagatta
     6181 aaaacctcaa ttccttccgc ttcttggagc aggcgattaa ttacgaagtg gaagcgcaaa
     6241 tcgagatttt ggaagacggc ggcaaagtac agcaggcaac catgctattc gaccctgaaa
     6301 aaggcgaaac ccgcgtgatg cgcctgaaag aagacgcgca cgactaccgc tacttccccg
     6361 atcccgattt gctgcccgtc atcatttcag atgcccaaat gcaaaaagcc aaagcagaaa
     6421 tgcccaagct gccgaaagaa atggcggcgc gtttcgtggc ggattacggc gtgtccgaat
     6481 acgacgcgcg cctgctgacc gcaagccgcg cacaggccgc ctattttgaa gaagccgcca
     6541 aagcaagcgg acaaggcaag ccgactgcca actggatgaa cggcgaactc gccgccgcgc
     6601 tgaacaaagc cggcctggaa cttgccgaca gcccgattac cgccccgcgc ctcgccgcgc
     6661 tggtgggcaa aatcgccgat ggcacattaa gcagcaaact ggcgaaaaaa gcctttgaag
     6721 ccatgtggga agaacccgaa gccaccattg ccgaaatcat tgaaaaacac ggtttgcaac
     6781 agatgaccga taccggcgcg attgaagcca tggtggacga agtgctggcc aacaacgcca
     6841 aagccgtgga acagtttaaa tccggcaacg aaaaagccct gaatgcgatt gtgggacaag
     6901 tgatgaaggc cagcaaaggc aaagcgaatc ccgcgcaggt gcaggagttg attaaagcta
     6961 aactggctta atccgttatc acacaggtcg tctgaaagca aagttccaac gaaggtaaaa
     7021 caggaaataa gctttcagac ggccttttaa cttaatctgc tcaaaccata ccaagacatg
     7081 aaccacaccg ttaccctgcc cgaccaaacc acctttgccg ccaacgacgg tgaaaccgtt
     7141 ttggccgctg ccgcccgaca aaacctcaac ctgccccatt cctgcaaaag cggtgtctgc
     7201 ggacaatgca aagccgaact ggtcagcggc gatattcaaa tggacggaca ctcggaacag
     7261 gctttatccg aagcagaaaa agcgcaaggc aagattttga tgtgctgcac cactgcgcaa
     7321 agcgatatca gcatcaacat ccccggctac aaagccgatg ccctacccgt ccgcaccctg
     7381 cccgcacgca tcgaaagtat tattttcaaa cacgatgtcg ccctcctgaa acttgccctg
     7441 cccaaagccc cgccgtttgc cttctacgct gggcaataca ttgatttact gctgccaggc
     7501 aacgtcagcc gcagctactc catcgccaat tcgcccgacc aagaaggcat tttggaacta
     7561 cacatccgca ggcgcgaaaa cggtgtctgc tcggaaatga ttttcggcag cgaacccaaa
     7621 gtcaaagaaa aaggcatcgt ccgcgttaaa ggcccgctcg gttcgtttac cttacaggaa
     7681 gacaacggca agcccgtcat cctgctggca accggcacag gctacgcccc catccgcagc
     7741 atcctgctcg accttatccg ccaaaacagc agccgcgtcg tccatttcta ctggggcgcg
     7801 cgtcatcagg atgatttgta tgccctcgaa gaagcacaag gattcgcatg ccgtctgaaa
     7861 aacgcctgct tcacccccgt attgtcccgc cccagagagt gctggcaagg aagaaaaggc
     7921 cacgtacaag acatcgcggc acaagaccac cccgacctgt cggaatacga agtatttgcc
     7981 tgcggctctc cggccatgac cgaacaagca aaaaatctgt ttgtgcaaca gcataagctg
     8041 ccggaaaact tatttttctc cgacgcattc acgccgtccg catcataatt ccccggtata
     8101 aagaggattc gagctttccg ttcagaacac aaaaaacttc ccgtccgtgt tttccccgtg
     8161 aaaaaatgcc gtctgaaacc cgattccggt tttcagacgg catatgtttt ttcctgttca
     8221 aggcgacagc cgctcgcgta tccagccacc atccagcaaa cggtattgga tgcggtcgtg
     8281 cagcctgctc ggtctgccct gccagaactc aagcaaatcg ggaatcacaa tatagccgcc
     8341 ccaatgcggc ggacgcggca cgtgcaaagg atgtttgagt ccgaccgccg ccgcctttgc
     8401 caccaatacc gccttgttcg gaatcacttc gctctgcgcg cttgcccacg cacccaaacg
     8461 gctctgatac gggcggctct caaaatattc gtccgacaat ttctccgcca gcctttcaac
     8521 acgcccttcc acgcgcacct gacgctccag ctccggccaa aaaaacgtca tcgccgcaaa
     8581 tggatgagca tccagcgaac gccccttgcg gctgtgataa ttcgtaaaga aaacaaaacc
     8641 ttcagaatta acttccttca gcagcaccat acggctgtta ggcctgccgc gtccgtcgac
     8701 cgccgccaca ttaaccgccg tcggctcgtt gacctgtgcg cgtaccgcct cgtccaacca
     8761 ccgctcgaac tgctcgatcg gattatcggc gcaatcggct tccgacaatt cccgtttgct
     8821 gtaatcttcc cgaatattgt gcaaatccat ttactgctcc tcttatcatt tgaaagattc
     8881 tactcccgca cgcaaaccga tttcaaccgt cgcacaaact ttgccccgac cccaagccgc
     8941 agcgacgatt tcatccgcaa aaccgccgca tcaggtacaa tatcgaaccg tccgaccgag
     9001 gacggcattt tatcaacccg tcctgccgca cacgccgcag aagaaccgcc ttatcaggcg
     9061 agttaggaaa aatgatgtcc aaacagccca ccagcaaacg ccaatggcgc gacggcgcag
     9121 ccccgtctgc caagaaaacc gccaaaccgt tcaaaagcaa agcccgtccc aaagatgaaa
     9181 cgggcaaaac cgcttcccaa ccttacggac aaaaagcttc agacggcatc aaacctcaaa
     9241 acgtccccaa acagcgcgcc gccaaagcca aaaaactcgt cgtccgcaat cccaaccaaa
     9301 aaattatgga acacgcgcgc gatttgaaag aacgccgcag cgacctgtcg cgcatggaac
     9361 ccgaacgcct gcaaaaagtg cttgccgcgt ccggcgtcgg ctcgcgccgc gaaatggaag
     9421 aatggattac caacggctgg ataacggtca acggcaaaac cgcgcaactg ggcgacaaag
     9481 ttacccccga cgaccacgtt accgtcaaag gcagcatcat caagctcaaa tgggcggacc
     9541 gcctgccgcg catcatcctg tattacaaac aagaaggcga aatcgtttcc cgtgacgacc
     9601 cgcaaggccg cgtcagcata ttcgaccgcc tgccgcaggc cgccagcagc cgctgggtcg
     9661 ccatcggacg cttggacatc aacaccagcg gacttctgat tcttaccacc tccggcgaac
     9721 tcgtccaacg tttcgcccac cccagcttcg aagtcgaacg cgaatacgcc gtgcgcgtct
     9781 tgggcgggct gaccggcgaa caaatgcgcg tcctcaccga agaaggcgtg atgctcgaag
     9841 acggcttggc aaaagtcgaa cgcatccgcg aacaaggcgg cgaaggcgcg aacaaatggt
     9901 acaacgtcgt gattaaagaa ggccgcaacc gcgaagtgcg ccgcattttt gaaagccaag
     9961 gactcaccgt cagccgcctc gtgcgcatcg gcttcggtcc catcggactg cccaaccgcc
    10021 tcaaacgcgg gcagttctac gaactcaacc ccgccgaagt cgccaacatc atcaaatggg
    10081 cggacatgct gctgccgggc gaacgccgcc gcaaaaaagc ctaaacccgc caaaacacaa
    10141 aaatgccgtc tgaaacatct gctgtttcag acggcatttt attcgggcgt tttcaggaga
    10201 aaaggtcgag tgctttgaca aagaccatca ccacgccgta ggcgagcggt atgacggcca
    10261 atgcccaacg ccaccagacc gatatgccgc cgccggaaac tttttcgccc acaaggtaag
    10321 cgtcggatat ggcggtttcg tcatcggggt tgccgctgtg tgcggcggtt ttgatgtctt
    10381 tttcgtggtg tttttcgtgt acggatttga cggcgaggtt gcacaacaaa ccgataatca
    10441 gcaggcacgc catgatgtac atggttacgc tgtatgcctg tgccgccggt atgccgctgt
    10501 cgatttggct ttggcgtatg taattgacca gtaccgggcc gatgacggcg gcggttgacc
    10561 aggccagcag gatgcgtccg tgaatcgcgc cgacctgata ggtgccgaac aggtctttca
    10621 ggtaggcggg aatggcggca aatccgccgc cgtacatgga aataatcacg caaaagccga
    10681 tgatgaacag ggctttgctg ccgccctcgc cgatggaggg aacggcgaaa tacagcagcg
    10741 aaccgagtac gaagaagatg gtgtaggtgt ttttgcgtcc gattttgtcg gaaacgctcg
    10801 accacaaaaa gcgcccaccc atattaaaca ggctcaggag gctgacgaag cctgccgccg
    10861 cacctgcgcc gactgccgcc tgcctaccta cggaggtttc ggaaaagagt tcctgaatca
    10921 tcacggatgc ctgacccaat acgccgatgc cggcagttac gttcaggcac aatacccaga
    10981 acaacagcca aaactgcggc gttttaatgg cttgggacac gttgacatga ttgctgctga
    11041 ccagcttgtt ttgcgttttc ggcgcggtat agccttcagg tttccagccg tcggcaggta
    11101 cgcggatggt aaacgcgcca aacatcatca gtgcgaggta aagcagaccc aatacggcga
    11161 aggtttcggc aaccccaacc gaagcagcgt ttgaaaaggc gttcatcagt gatacggaaa
    11221 gcggcgaggc cagcattgcg ccgccgccga aacccataat cgccaaaccg gtcgccatac
    11281 ccggcttgtc gggaaaccat ttcatcagtg tggaaaccgg cccgatgtag cccaaaccca
    11341 agcctacgcc gccgatgacg ccgttgccca aatagagcag gaagaggttg tgcgtacgca
    11401 cgccgaatgc ggatacgaag aagcccaggc tgaagcagca ggcggcggca aatatggctt
    11461 tgcgcggccc tacccgttcc atccacgtac caaacagggc ggccgacgcg cccagcatcg
    11521 cgagtgcgat actgaaaatc caacctacgg tcgtcagctt ccaatctccg gccgccgatt
    11581 cggttatgcc gataagtttg gtcagcggcg cgttgaatac ggaataggcg taaatctgcc
    11641 cgatggcaag gtgcaccgcc aatgctgcgg gcggtacgag ccaacggttg aaacccggct
    11701 tggcaatgct tgcctcacgg tctaaaaact tcataacatc ctctttctgt cagttgaaaa
    11761 ataaaatttc atttgctcaa tggaaactta ttgaaaacta taaaaaaata tcggatcggt
    11821 tttttatccg ccccaagatg ccccgtctga aacatttcgg gtgtacggaa aggtttctgt
    11881 tttttccgac aaattcctgc ggcttttcgc ttccggattc ccgcttttgc gggaatgacg
    11941 aattaaagat tatcttaagg tcaagggact ggattcccgc ttttgcggga atgacggcgg
    12001 cgggttgctg ttttttgttt ataaatttct gtaactttgg atttcagcca tcttcaataa
    12061 attatcgaaa cgtatcaatc gtcattcccg cgcaggcggg aatccatcca gttcgttcga
    12121 tttcggtttt ttaagtttcg agtaacttct gactcgtcat tcccgcgtag gcgggaatcc
    12181 agttttttga gtttcagtca tttctaatac attcccacca cttttcgctt ccggattccc
    12241 gctttttcag gaatgacgaa ttaaagatta tcttaaggtc aagggactgg attcccgctt
    12301 tcgcgggaat gacggcggag ggggagcggt ttttccgatt gggtttaaat gcaatcgaac
    12361 aaatcctgct gcccttgttc tttgcttacg cgcacgtcgg tttcgccgtc ggcgaaggta
    12421 atgtgcagct tctgcccctg cttcaaaaca tcggcgttgc ggatgacttg tccgcgtgta
    12481 tctttgacga cggaaaagcc gcgttccaaa atgtgctgcg gcgaaacggc ttcgagcaat
    12541 gcggcttggg cggtcaggct ttggcggcgg tgggtaagca gttggctgaa ggcggcgggc
    12601 aatgccgtct gaaggcggac gatgttgttt ttgcaaacgg aaacatcagg acggtaatgt
    12661 tgcagggctt gggtttggcg ttcaaaacgg gcggtgtggg cacggaggtt ttgcttcata
    12721 gagtaagaca gcgtttgcgc cagcttgccg attgaagcgc gctgctcatc aagtttttgg
    12781 cgcggatgac ggatttgccg cgcaagccag tcgagttttt ggctggcatc gaaatagcgt
    12841 tgttccaaaa cggttttcag acggccttgc gcctgagcga gacgatgtag cgattcttgg
    12901 cggttggggc tgaccagctc cgccgcaccg gtcggcgtgg gcgcgcgcac gtcggcgaca
    12961 aaatcggcaa gcgtgaaatc ggtttcgtgt cccacgccac tgacgacggg aaccgtgcag
    13021 gcttcgattg cgcgcacgac cggttcttcg ttaaacgccc acaagtcttc aatgctgccg
    13081 ccgccgcgac agacaatcaa cacgtcgcat tcggcgcgtt gcgaggcggt tttaatcgct
    13141 tgtgcgattt gtaactcgct gcctgtgcct tgaacaggtg tcggataaac gataacgggg
    13201 atttcgggtg cgcggcgttt caaggtagta acgacatcgc gcaaagccgc cgccgccaga
    13261 ctggttacga tgccgataca ttgcggtcgg gcgggcaaag gtttcttgcg ttccgccgca
    13321 aacgcgcctt ccgcctgcaa ctgcgccttc aaccgctcgt aggcttcgta aagctgcccc
    13381 aagcccttga gccgcacttc gttcacggta atctgaaatt cgccccgcgt ttcataaata
    13441 ctgattttgc ccgccacttc gatatggtcg ccttctttca aaggtttcgc caaacgcgcc
    13501 gccgcaccct tgaacatcgc gcaacgcacc tgcgcgcggc tgtctttgag cgagaaataa
    13561 taatgcccgc tggcggcacg ggtcaggttg gacacttcgc ccgcaatcca cagaccggca
    13621 aggtggtttt ccaaaatact tttggcaaat gcgttcaact cggaaacgga caacacgtca
    13681 gaatggaaaa aatcagacat cgaatcaatc aaatagtaaa aaatatgaat atgttttgaa
    13741 gcccaaggcg gcatagagcc gcctaaattg tcaacaatat tataacacgc gccatcttgc
    13801 cgcccgcctt ttcccatatg acttttttaa gcggggaatg ggaaaaatat tcatcaacct
    13861 gcctgcaatc tattcaaatt gaaccgccga caagctatga tgcggatatt ttcgacagga
    13921 ggaaaaatgg atacgcaggc agttatcaca catatcgccc gatggttgga cgaatacgcc
    13981 gcacgggcaa atgcaaaagg gtttgtcgtg ggcgtttccg gcggcatcga ttccgccgtc
    14041 gtctccgcac tcgccgcccg caccggccgc cccacgctgc ttctggatat gccgatacgc
    14101 caacaccccg accagcttga gcgggcaagg ctgcacatcc gcaatctgca acggcaatat
    14161 gccaatgtaa gcgcgcaaac ggtcgacctg accgacacct tccagacctt tgaacaaacc
    14221 gtcggcattc atcagacggc atttgacaat cagccgcttt ccctcgccaa cgccagaagc
    14281 cgcctgcgta tgctgaccct gtactactac gggcagatac acggactgct ggttacgggg
    14341 acaggtaata agattgaaga tttcggcgtg ggctttttta ctaaatacgg cgacggcggc
    14401 gtagacatca gcccgattgc cgacctgacc aaaacgcagg tttaccggct tgccgaagca
    14461 ttgggcgtgg acgaggcgat tcaaaaagcc ccgccgaccg acggcctgtg ggatacggaa
    14521 cgcaccgacg aagaacagat gggcgcaagc tatcccgaac tggagtgggc aatgggcgtg
    14581 tacggcacgc gcaaacccga agattttgaa gggcggcagc gcgaagttct ggaaatctat
    14641 acgcggcttc accgcgccat gcagcacaaa atcaacccga ttcccgtgtg ccgcatcccg
    14701 cccgaattgt tgggctgaac gtgaaaatgc cgtctgaagc ggaaaaccgt gtttcagacg
    14761 gcatggaaat atccggacgc gctccttaag aatcgagtac gcgggcaaac agaatatcgt
    14821 tttccaaatg aatgtggtcg ttcaaatcct ccaccatttc tttcgccagc gcgtaaagcc
    14881 gcgtccagct tccgcaagcc ccttctggcg gttggaaatt gtcggtcagc tctttgagcc
    14941 gtgcgatggc gcggtcgtgt tcttcgtgtt cgtgcatcat cacgctgatg ggcattgccg
    15001 caccgcgtcc gacaccctga ttaatcatcg gaaacagcat cctttcctct ttcatcatat
    15061 gcatcagcag ttcgttctgc atatcggcaa gcagcccggc aatttccgcc ggaaaggtat
    15121 cggcatggac ttggaccact ttctgcgcca gcggcaccaa ttcttcaaat tgtgcgcggt
    15181 ggacattgtg gtagcgttgc aggatataat cgacggttgc gccaaagggg gctgtttccc
    15241 aaacggaaaa atcagtcatt gcagtgttcc ttttataggg tttcgggttt ggttttgaac
    15301 attcatactt taagaatcta tttaaacgga gcatacaccg cccgcgcgtt tctgtacagc
    15361 ttcaaaaaca ttccttacac tttgataata aaagtaattt tcaaaaataa aatactgtcc
    15421 gaaccgtttt ttagaatttg caaaggcgat tggggcggta cagaaaaact attatcccgc
    15481 ccgcccactt gaaattttta tgcccaagcc ctatcctgca cgctatcgtg ccaatcccaa
    15541 ccgaaaagga aaaataatga gcagcgaatt gattatacac accagcgatg ccgcttttga
    15601 aaaagacgtt ttaaattcag atacccccgt cctgctggac ttttgggctc cgtggtgcgg
    15661 cccctgcaaa atgattgccc cgattttgga cgacatcgcc gccgaatttg aaggccgtct
    15721 gaaagtggtc aaaatcaaca tcgacgacaa cgaagccacc ccgtcccgtt tcggcgtgcg
    15781 cggcattccg accctgatgg tgttcaaaaa cggcgaagtc gtggctacca aagtcggcgc
    15841 attggcaaaa ggtcagctga ccgcctttgt cgaagcctcc atcgcctgat aaagcgcaat
    15901 cgaaaaagcc gccggaagat tccggcggct ttttcgcacc cttaagattt gtggcggatt
    15961 tcccagcacc tatggatttt tttgttgcgg aaatcttcgg gaacggattg tttggaaatg
    16021 tctttgacgg cgtattgttc cgataccaag tcgtctaaga cgaagctgcg caggttgttg
    16081 gaaaagtaca aaatgccgtc tgaagcgagc agcttcaccg cgccgtcaat cagctttttg
    16141 tggtcgcgct ggatgtcgag aatgtcgagc atctttttgc tgttggagaa gctgggcggg
    16201 tccatcacaa tgaggtcgaa ccgcctgcct tccccatatg ccgtctgaag atattgaaac
    16261 acgtcggcgc ggacgatttt gtgtcgttcc gtatcgatgc cgttcaattc aaaattgcgt
    16321 ttcgcccaat caagatatgt gttggacaaa tcgacggttt cgctggatgc cgcgccgccg
    16381 gtggcggcat agacggtgaa gctgccggtg taggaaaaca ggtttaaaaa acgtttgccc
    16441 gccgccgttt cgccgacttt tttgcgcgtg tttcgatgat ccaaaaaaag ccccgtatcc
    16501 aaatatttat caaggttgac ccaaaacttg cggccgtttt cggtgatgac gaaatcgtcg
    16561 cccgccttgc cggttttctc gtactgctgc aaaccttttt ggcgttcgcg gcgtttgagg
    16621 cggatttgtt cgggcgcaaa accggtaacg aaagcgacgg cttccaagac ttcggcaagc
    16681 cacgcttcgt attcttcggg ccgcatcagc cagccggtat cgtattcctg aaggtggatt
    16741 cgatcgccgt aaacatcggc ggcaaagggg aattggggga tgtcgcggtc gtaaatgcgc
    16801 caggcttcga tgccgttgcg tttcacccat ttcataaggt gtttgatgtt tttgcccaag
    16861 cggttggcaa acggtgtgat gtcggtcatt ggtttcaggc ggaataaagt ggaaaacggc
    16921 aattttactg taattaacgc ccgattgctt gaccgtttcg ggcaaaccct ataccatccg
    16981 tcgcttatct tgtcatacga agccatcgcc ttccaaccta aaccgccctt acgggcgcgt
    17041 ttcttctgtt gctttgattt tgcaaagcat atctgtgcag gttgccgtcg atgtaaacca
    17101 caagcaagcc gcttgcgaca accctgtaac ttcacattcc ccgtatcgtt acccttccct
    17161 gcttcaggcc gtctgaacct ttcggacgcg ggcgttgttg tcttccaagg atagccatgt
    17221 ctattaaatt tgccgatttg aaccttgata aaaacatttt gtccgccgtc agcagcgagg
    17281 gttacgaaag cccgacgccg attcaggcgc aagccattcc gtttgctttg gaaggccgcg
    17341 acatcatggc ttcggcgcaa accggctccg gcaaaaccgc cgcctttctg ttaccgactt
    17401 tgcaaaaact gaccaaacgc agcgaaaaac cgggcaaagg cccgcgtgct ttggtgttga
    17461 ccccgacccg cgaactggcg gctcaagtcg agaaaaacgc gctggcgtat gccaaaaata
    17521 tgcgttggtt ccgcaccgtc agcatcgtcg gcggcgcatc gttcggctac caaacccgcg
    17581 ccctgagcaa accggtcgat ctgattgtcg ccacgccggg ccgtctgatg gacttgatgc
    17641 aaagcggcaa agtcgatttt gaacgtttgg aagtgctgat tctggacgaa gccgaccgta
    17701 tgttggatat gggctttatc gacgacatcg aaaccatcgt ggaagcaacg ccgagcgacc
    17761 gtcagacttt attgttctcc gccacttggg acggcgcggt cggcaaactg gcacgcaaac
    17821 tgaccaaaga ccctgaaatc atcgaagtcg aacgcgtgga cgaccaaggc aaaatcgaag
    17881 aacaactgct gtactgcgac gatatgcgcc acaaaaaccg cctgctcgac cacatcctgc
    17941 gtgatgccaa catcgaccaa tgcgtgattt tcacgtccac caaagccatg accgaagtca
    18001 tcgcggatga attgtacgaa aaaggtttcg ccgccaactg cctgcacggc gatatgccgc
    18061 aaggctggcg taaccgcacg ctgatggact tgcgcaaagg ccgctgcaaa attttggttg
    18121 ccaccgacgt cgccgcacgc ggtatcgacg taccgaccat tacccacgtc atcaactacg
    18181 acctgccgaa acaggcggaa gactacgtcc accgcatcgg gcgcaccggc cgcgcaggcc
    18241 gcacgggtat tgcgattacg tttgccgaag tgaacgaata cgtcaaagtc cacaaaatcg
    18301 aaaaatacat taaccgcaaa ctgcccgaac tgaccatcga aggcatggaa ccgacacgca
    18361 aacgcaaacc ggccggcggc aaaccgaaag gcaaaggcgg ctggggcgat cgtaaagccg
    18421 gcggccggcg cggcgatcat aaaccgggca aagaaggctt cggcggcaaa acgcgcggcg
    18481 aaggtttcaa gaaagaaagc tttaagaaag acggtttcaa aaaaaccggc gaaggcttca
    18541 aaggcaaacg caaagccggc gattcttttg caggcagagg cgaacgccgt tacaaagacc
    18601 gctaagcccc aacctgccgc ataaaccaat gccgtctgaa accgatttcg agtttcagac
    18661 ggcatttttg caatgtttca gcaccgcccg gctttgatac ccaaaggatt aatctgcaat
    18721 atagtggatt aacaaaaacc agtac
//
LOCUS       DAWFJR010000033        18410 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWFJR010000033 DAWFJR010000000
VERSION     DAWFJR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18410
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.033"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(255..1358)
                     /locus_tag="WGR04_001052"
     CDS             complement(255..1358)
                     /locus_tag="WGR04_001052"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ0794621.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGAGAFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(1437..2993)
                     /gene="dnaA"
                     /locus_tag="WGR04_001053"
     CDS             complement(1437..2993)
                     /gene="dnaA"
                     /locus_tag="WGR04_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ0794622.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     VSEEMPVQEVLLDELPSEEPAKSVQAKTAADILAERMKNLPHEPRHTAGSASRPESEV
                     LAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQTIGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMYGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            3281..3415
                     /gene="rpmH"
                     /locus_tag="WGR04_001054"
     CDS             3281..3415
                     /gene="rpmH"
                     /locus_tag="WGR04_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ0794623.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            3418..3783
                     /locus_tag="WGR04_001055"
     CDS             3418..3783
                     /locus_tag="WGR04_001055"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ0794624.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRSTAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            3848..4069
                     /gene="yidD"
                     /locus_tag="WGR04_001056"
     CDS             3848..4069
                     /gene="yidD"
                     /locus_tag="WGR04_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ0794625.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            4241..5878
                     /gene="yidC"
                     /locus_tag="WGR04_001057"
     CDS             4241..5878
                     /gene="yidC"
                     /locus_tag="WGR04_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ0794626.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDNDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCSIDI
                     KRRNDKLYSASVSVPLAAIPAGAKAETAVNLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(5959..6672)
                     /locus_tag="WGR04_001058"
     CDS             complement(5959..6672)
                     /locus_tag="WGR04_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ0794627.1"
                     /translation="MMSPVLYLIPTPLGTPDTPCLLPHEQQAVVGLTDFVVEAEKTAR
                     AHLKHLGVTMPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGA
                     NLVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQSLKALEQ
                     RSRQQNETQLFIETPYRNDVLLADAVENLHSETRLCTATDLTLPTQEIISQTVVQWRK
                     RKEMPNLKKRPTIFVMYAG"
     gene            complement(6731..7321)
                     /gene="maf"
                     /locus_tag="WGR04_001059"
     CDS             complement(6731..7321)
                     /gene="maf"
                     /locus_tag="WGR04_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ0794628.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEAL
                     GALLIERIESSDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            7469..7972
                     /locus_tag="WGR04_001060"
     CDS             7469..7972
                     /locus_tag="WGR04_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ0794629.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSS"
     gene            8006..8185
                     /gene="rpmF"
                     /locus_tag="WGR04_001061"
     CDS             8006..8185
                     /gene="rpmF"
                     /locus_tag="WGR04_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ0794630.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            8360..8887
                     /locus_tag="WGR04_001062"
     CDS             8360..8887
                     /locus_tag="WGR04_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ0794631.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            8975..10030
                     /gene="plsX"
                     /locus_tag="WGR04_001063"
     CDS             8975..10030
                     /gene="plsX"
                     /locus_tag="WGR04_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ0794632.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDESPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENADGL"
     gene            10166..10417
                     /locus_tag="WGR04_001064"
     CDS             10166..10417
                     /locus_tag="WGR04_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ0794633.1"
                     /translation="MKTLEKRMKALDKQMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            10668..10859
                     /locus_tag="WGR04_001065"
     CDS             10668..10859
                     /locus_tag="WGR04_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794634.1"
                     /translation="MIQTILEDGDIEVKNLKTENYSGKAREILGDSYPIEHISKNIST
                     IQNIISEFMIQKQTPAPII"
     gene            10879..11082
                     /locus_tag="WGR04_001066"
     CDS             10879..11082
                     /locus_tag="WGR04_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794635.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PVSVICTLSVLGNHRFFAYNSRI"
     gene            11109..12071
                     /locus_tag="WGR04_001067"
     CDS             11109..12071
                     /locus_tag="WGR04_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ0794636.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            12118..12438
                     /locus_tag="WGR04_001068"
                     /pseudo
     CDS             12118..12438
                     /locus_tag="WGR04_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
     gene            12619..13545
                     /gene="fabD"
                     /locus_tag="WGR04_001069"
     CDS             12619..13545
                     /gene="fabD"
                     /locus_tag="WGR04_001069"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ0794637.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFAEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAADA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKQICAEAAQGEVVEAVNFN
                     SPGQIVIAGNAAAVGRAMTAAKEARAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKTAACSALTDAGQIAAFIEAH"
     gene            13683..15548
                     /gene="msbA"
                     /locus_tag="WGR04_001070"
     CDS             13683..15548
                     /gene="msbA"
                     /locus_tag="WGR04_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ0794638.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSIIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAINRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLTPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDASPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            15648..17213
                     /gene="guaA"
                     /locus_tag="WGR04_001071"
     CDS             15648..17213
                     /gene="guaA"
                     /locus_tag="WGR04_001071"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ0794639.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDGEPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            17275..18021
                     /gene="fabG"
                     /locus_tag="WGR04_001072"
     CDS             17275..18021
                     /gene="fabG"
                     /locus_tag="WGR04_001072"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ0794640.1"
                     /translation="MSTQDLSSKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTMHVNGGMLMP"
     gene            complement(18283..18410)
                     /locus_tag="WGR04_001073"
                     /pseudo
     CDS             complement(18283..18410)
                     /locus_tag="WGR04_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4618 a         5569 c         4383 g         3840 t
ORIGIN      
        1 gtcttgcgaa aattatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca
       61 gtacaaatag tacggaaccg attcacttgg tgcttcagca ccttagagaa tcgttctctt
      121 tgagctaagg cgaggcaacg ccgtactggt ttttgttaat ccactatacc tttccgacag
      181 ccgaacaaaa ccccgaatcc gtctgcacgg ttcggggtat atctccaata cgggcatcgt
      241 gttccggaaa accgtcaaat ccgcatcggc atcacaatat atttgaaatt cggattgttc
      301 ggcacggtaa acagcgtcga gcggttggca tcgccgaagg caagctgcat atcgtcggaa
      361 tggatgttgc gcaacacgtc catcagatag ccgatattga aaccgacttc gagttcgccg
      421 ccctgatagg caatctcgat ttcttcgcgc gcttcttcct gctcgttgtt gctgcacacg
      481 acgctcaaca ggccggattg caggaacagg cgcgcgccac ggaatttttc gttggcaaga
      541 atggacacac gttccagcgc gcccaaaagt tcggcacggg acaaaacgaa aatcttgtcg
      601 ttgtccaaag ggatgacgcg gttgaaatcg gggaatttgc cgtcgataac cttgctgacg
      661 atggtcgtgc cgttgcattg gaaacgcacc tgcttgtcca gcagctcgat ttgaatcgga
      721 tcgtcggggt tgttcaacag tttgaacagt tccagcaccg ttttgcgcgg caaaatcact
      781 tcggcgcgcg gcaaatccgc atcaatcgcg caggctgcat aggcaaggcg gtgtccgtcg
      841 gtcgccacaa ggcgcaactg gctgccctca acctgcatca gcagaccgtt gagataatag
      901 cggatgtcct gcaccgccat gctgtactgc acttgcgaca gcatggtttt gaaacgctcc
      961 tgctccagcg agaaagtcgc gctgatgtcc tcgccgacat tcatcatcgg aaaatcggcg
     1021 gcaggcaggg tctgcagggc aaaacgcgat ttgcccgctt tcagcgtcag acggctgtcg
     1081 tcccaatcca gcgacaccag cgcaccggcg ggcagtgccc gtaaaatatc ctgaaatttc
     1141 ttggcattgg tggtgatgcg gaaggcgccc gcgccgccct cgggacccgc agtgtcgatt
     1201 tggatttcca aatcggttgc caagagtttg gtctgaccgc cttttccctc aatcaggacg
     1261 ttggacagga tgggcagggt gtggcggcgt tcgacaatgc cggtaacggc ttgcaacggc
     1321 ttgagcaggc tgtcgcgctc ggcttgtaaa atcaacattt tcgctccttt aatcggttgg
     1381 attttttacc cttcggacgg catgggcaat atcatgccgt ctaaaaacgt tttccatcag
     1441 ttttgaatca gaatcagcag cttttcataa tcctgagcca attccggatc ttcttcgcgc
     1501 agtttcgcca ctgccctgat gccgtacata acggtcgtat ggtcgcgccc accaaacgaa
     1561 tcgccgatag acggcaggct caaagtggtc agttctttgg tcaggctcat ggcaacctga
     1621 cgcggacggg caatgttgcg cgtgcgtttt ttgccgagta cgtcgctgat tttgatgcgg
     1681 taatatttcg ccaccgcatc gatgatgata tcggcggtga tgactttgtg cttctcggca
     1741 ataatgtcct gcaaagcggt acgcgccaaa tcgatgtcga tgacgggacg gttcataaag
     1801 cggctgctcg ctccgacacg gttaaacgca ccttcaagct cgcgcacgtt ggaacggatc
     1861 agattggcaa tgaacaacgc ggcttcgtct tcgatactga tgcccgccgc ttccgccttt
     1921 ttctgcaaaa tagcgatacg catttccaat tcgggcggct cgagttccaa agtcagtccc
     1981 catgaaaaac gggatttgag gcggtcgtcc ataccttcga ttttggcggg taaaacatcg
     2041 caagtgagga tgagctgttt tttctcgttg tggaaatggt tgtacagata gaaaaactct
     2101 tccatcgtac ggtctttgcc tttgatgaac tggatgtcgt cgataatcag caggtcgtat
     2161 tgcttgtatt gctgtttgaa cacgtcgtaa gtgttgttgc gcacggcttt catgaagctg
     2221 cggatataat cgtccgaatg catatagcgt actttcgcgt cgggacggtt tttcaaaagc
     2281 tcgttgccga tggtctgaac caagtgggtt ttacccaaac ccgtactgcc atagaggaaa
     2341 aacgggttgt aactctgccc cgggctttcc gcaatcgcct gcgccgccgc cgccgcaagg
     2401 cggttgccct tgccttctac caacgtatca aatgtgtaat ccggagacag gttggtttgt
     2461 tcgtaacgcg cctcttccgc atcacgctgc gcatccgtcc gcgctttcgc aagtacctcc
     2521 gattccggac gggaagcaga cccggcagta tggcgcggct catgcgggag gtttttcata
     2581 cgttccgcca aaatatccgc cgccgttttc gcctgtacgg atttggcagg ctcttcagac
     2641 ggcagctcgt ccaacagaac ctcctgcacg ggcatttcct ccgacaccgc gtgcaaggac
     2701 ggctcggcag gttcgacagc accttcaacc gccgccatct cataacgcac gccttctccc
     2761 ggtttgaata cgaaggcgga acggccggca gccaactctt ccctcaccgc ttctattttt
     2821 ccggcaaact ggctcttgag catattgcag gcaaactggt tcttgccgta caccacccat
     2881 acgccaccct cctcaccgac ggtaaggggc gcaatccatt gcgcaaactg cccttgaggc
     2941 aacatatcgt gaagacggcg gaggcacagc ggccaaaact ctgctaatgt catggatagg
     3001 ctcgaatcgg taaaaatgaa atcgaaaaca aagaaaatat aatattttca aaaagaaaac
     3061 aaatctgttg aacgcacatc ggttcaaaac gcgactgccc gattataccg actcacgaat
     3121 attttatcca caacccgtgc aaaaatttat ccacagaaag gcggcggaaa tccgcaggca
     3181 atcgggcaat cttcctgcaa agtttctata ttgattgaca aaagcggcaa attggagtgt
     3241 aattcacggt ttatctatct accgattttt aggaaacatc atgaaacgca cttatcaacc
     3301 ttccgttacc aaacgcaaac gcacccacgg cttcctggtg cgctccaaaa cacgcggcgg
     3361 ccgcgcagta ttggctgcac gccgcgccaa aggccgcaaa cgcctagcgg tataattttg
     3421 gactaccgct tcggaaggca gtaccgcttg ttaaaaacgg atgatttttc atccgttttt
     3481 gcattcagaa accgccgcag ccgcgacctg ctgcaagttt cgcgttcaaa cggcaacggg
     3541 ctggaccatc cccgcatcgg tctggtggtc ggcaaaaaaa ccgccaaacg cgccaacgaa
     3601 cgaaattata tgaagcgcgt tatccgcgac tggtttagat tgaacaaaaa ccggctgccg
     3661 ccgcaagatt tcgtcgtgcg cgtccaccgt aaattcgaca ggtctaccgc aaaacaggca
     3721 agggcggaac tggcacaact catgttcggc aaccccgcaa ccggatgcag gaaacaggca
     3781 tgatcagaac ggtactctgc aggcaaggtt cagacggcaa cgggtttccc atacaaggaa
     3841 catcccgatg aacttcctat tgtccaaact cctgctggga ctgatacggt tctaccaata
     3901 ttgcatcagc ccgctgattc cgccgcgctg ccgctatacg ccgacctgtt cgcaatacgc
     3961 ggtcgaagcg gtcaaaaaat acggcgcatt caaaggcggc cggctcgcca tcaagcgcat
     4021 tgcacgctgc caccctttcg gcgggcacgg acacgacccc gttccctgac ccgacgcaat
     4081 attcaaattg cacgctttcc ttttatttcc catcggtttc tatataatgc cgtctgaaac
     4141 ttcgggctgg cggcacgacc gtcgggtatg aagcccgccc ttattccccg tctatcggaa
     4201 cacgcaacct gcggcatttc cgaccattca ggaaactctt atggatttta aaagactcac
     4261 ggcgtttttc gccatcgcgc tggtgattat gatcggctgg gaaaagatgt tccccactcc
     4321 gaagcccgtc cccgcgcccc aacaggcagc acaacaacag gccgtaaccg cttccgccga
     4381 agccgcgctc gcgcccgcaa cgccgattac cgtaacgacc gacacggttc aagccgtcat
     4441 tgatgaaaaa agcggcgacc tgcgccggct gaccctgctc aaatacaaag caaccggcga
     4501 cgaaaataaa ccgttcatcc tgtttggcga cggcaaagaa tacacctacg tcgcccaatc
     4561 cgaacttttg gacgcgcagg gcaacaacat tctaaaaggc atcggcttta gcgcaccgaa
     4621 aaaacagtac agcttggaag gcgacaaagt tgaagtccgc ctgagcgcgc ctgaaacacg
     4681 cggtctgaaa atcgacaaag tttatacttt caccaaaggc agctatctgg tcaacgtccg
     4741 cttcgacatc gccaacggca gcggtcaaac cgccaacctg agcgcggact accgcatcgt
     4801 ccgcgaccac agcgaacccg agggtcaagg ctactttacc cactcttacg tcggccctgt
     4861 tgtttatacc cctgaaggca acttccaaaa agtcagcttc tccgacttgg acaacgatgc
     4921 caaatccggc aaatccgagg ccgaatacat ccgcaaaacc ccgaccggct ggctcggcat
     4981 gattgaacac cacttcatgt ccacctggat cctccaaccc aaaggcggac aaagcgtttg
     5041 cgccgctggc gactgcagca tcgacatcaa acgccgcaac gacaagctgt acagcgcaag
     5101 cgtcagcgtt ccccttgccg ccatcccggc cggcgcgaaa gccgaaaccg ccgtcaacct
     5161 gtatgccggc ccgcaaacca cttccgtcat cgcaaacatc gccgacaacc tgcaactggc
     5221 caaagactac ggcaaagtac actggttcgc ctccccactc ttctggctcc tgaaccaact
     5281 gcacaacatc atcggcaact ggggctgggc gattatcgtt ttaaccatca tcgtcaaagc
     5341 cgtactgtat ccattgacca acgcctctta ccgctctatg gcgaaaatgc gtgccgccgc
     5401 gcccaaactg caagccatca aagagaaata cggcgacgac cgtatggcgc aacaacaggc
     5461 gatgatgcag ctttacacag acgagaaaat caacccgctg ggcggctgcc tgcctatgct
     5521 gttgcaaatc cccgtcttca tcggattgta ttgggcattg ttcgcctccg tagaattgcg
     5581 ccaggcacct tggctgggtt ggattaccga cctcagccgc gccgacccct actacatcct
     5641 gcccatcatt atggcggcaa cgatgttcgc ccaaacttat ctgaacccgc cgccgaccga
     5701 cccgatgcag gcgaaaatga tgaaaatcat gccgttggtt ttctccgtca tgttcttctt
     5761 cttccctgcc ggtctggtat tgtactgggt agtcaacaac ctcctgacca tcgcccagca
     5821 atggcacatc aaccgcagca tcgaaaaaca acgcgcccaa ggcgaagtcg tttcctaaat
     5881 gccgcagcat gaaaaatgcc gtctgaaacc tgttcagacg gcatttttat tgttcacccc
     5941 ctatcggggc ggaaatcttc aacctgcata catcacaaaa atcgtcgggc gttttttcag
     6001 attgggcatt tcttttcttt ttcgccactg cacaaccgtc tggctgatga tttcctgagt
     6061 cggcaacgtc aaatccgtag ccgtacacaa acgcgtttca gaatgcaggt tttccaccgc
     6121 atccgccaac aacacatcgt tgcgatacgg cgtttcgata aacagctgcg tttcattctg
     6181 ctggcgcgaa cgttgctcca aagccttcaa actctgaatg cgctcatttt tttcagacgg
     6241 cagataaccc ttaaacgcaa aactctgccc gttcgcaccc gaagccatca aagccagcag
     6301 caggctggaa ggcccgacca gcggacgcac ttcaaaaccg tgtttatgcg ccaatgccac
     6361 caaattcgca cccggatcgg caacggccgg gcaacccgcc tcgctgacaa tgcccatact
     6421 gcgcccttct tgcaaaggtt tcagcaattc cggcaaagtc ttcaaatccg tgtgttcgtt
     6481 cagcgtttgc aaattcagtt cgcggatagg cattgtcacg cccaaatgtt tcaaatgcgc
     6541 gcgcgccgtt ttttccgctt ccacgacaaa atccgtcagt ccgacaaccg cctgctgttc
     6601 gtgcggcaac aggcacggcg tgtcgggcgt gcccaaaggc gtaggaatca aatacaaaac
     6661 aggagacatc attccctcac tcgtcagtta aaaatgccgt ccgaaccctt cagacggcat
     6721 aaacgggcag ttacagaacc tccacgccct catttttcaa gaaatcgacc agacggaaaa
     6781 ccggcaaacc gattaaagca ttcggatcgc tgctttcaat cctttcgatc aacaatgcac
     6841 ccaaagcctc gctcttcaac gcacacgagc aataaaccgc atcaggctcg cgctccaaat
     6901 agcggaggat gtgtagctcg tccaacttcc tcatcacgac caccgtctta tcaatatgcc
     6961 gctgcatcct gcccgtaacc gtatttagca gaacgaccgc gctgtaaaac tcaatctccc
     7021 tgccgctcaa atgcatcaac atcttttgcg cattagcaag gttcatcggc ttgccccact
     7081 gcctgccgtc gcaccacgcc acctggtccg caccgacaat caacgcctcg gggaaacgtc
     7141 ctgccaacga ccgcgcctta ccctcggcaa ggcgcaatgc cgtctgaggg gcagactccc
     7201 ccaacatcgg cgtttcgtca aaatcggggg acgccgcctg aaaggcaatg ccgagccttt
     7261 ccatctgttc gcggcggaaa accgaactcg tacccaaaat caaaggcagt tccaaaccca
     7321 tcccatcctc cttaccgttg aaaacacgcc cgaaggggcg gtaaaatcca gccatgcgcc
     7381 gaaacacgga tacccgcctt cggcgtaccg caacattttt cttaaaaata ttgacgttag
     7441 aacatctaaa ttatatcata tctcgtttat gtcagaccct aatttgattg acccggaaat
     7501 tttcgccgcc gaagggcaga atctgcaagg cagttttctg ctggaagaat tggatgaacg
     7561 cgtcagttcg cacgattatc ccgccgacag gcagaccaaa atatcgttta cactgaccgg
     7621 cggtcgcgat cggctgcaac gcctgttcct cgacctgaac gtcaaagccg atatgcccct
     7681 gatttgccag agatgtatca aacccatgcc gttcatgctt gatgaaagca gccgtatcgt
     7741 cctgttttcc aacgaagagt ccttggacga atccatgctt gccgacgaag aactcgaagg
     7801 catactgatt gaaaaagaac tcgacgtgcg cacattggta gaagaccaaa tcctgatgtc
     7861 cctgcccttt tcgccgcgac acgaagactg cggcgacaat gggacactgg aagaagtcaa
     7921 tcgggacaaa cccaacccct ttgctgtttt ggcaggtttg aaaagcagtt aattaggaca
     7981 cagtttattt atctaggagc ttgaaatggc cgttcaacaa aacaaaaaat ccccttccaa
     8041 acgcggtatg caccgttcgc acgacgcgct gaccgcgcct gcactgtctg tcgacagcac
     8101 aaccggcgaa gtacaccgcc cgcaccacat ctcccccaac ggtatgtacc gcggccgcaa
     8161 agtggtcaaa gccaaaggcg aataatccct attcgactga ctgaaaaagc cagaacattg
     8221 ccatgcaatt actggctttt tttgcattgg acgcaccatc cgtccaaact ttcgccatac
     8281 gtcaacacac aggggcaaag cgttccgtat aataccccgt gaaaatattc caaaagcccc
     8341 aaccaccaag gaaattccga tgaaacagaa aatctggtac acctacgatg acatccaccg
     8401 cgtcatcaaa gcattggcag aaaaaatccg gaacgccgac atcaaatacg atgccatgat
     8461 tgccatcggc ggcggcggct ttattccggc acgtatgctg cgctgttttc tggaaattcc
     8521 gatttatgcc gtaaccaccg cctattacga cagcgacaac gaaggacagg ttaccgaaga
     8581 agtcaaaaaa gtccaatggc tagaccccgt tccagaagcc ctgcggggca aaaacgtact
     8641 cgtcgtcgat gaagtggacg acagccgcgt aaccatggaa ttttgcctga cagagctgct
     8701 caaagaagac ttcggtacga tcggcgtcgc cgtactgcac gaaaaaatca aagccaaagc
     8761 aggcaaaatc cccgaaggca ttccctattt cagcggcatc accgtagaag actggtggat
     8821 caactatccg tgggacgcac tcgacatcga cgaacacaac cgccttgccg aggccggccg
     8881 aggctgaccc tttcagacgg catattttcc gaaccgatgc cgtctgaagc ccgcacgacc
     8941 cctgccgcag accgaaaacc taccggagaa ccctatgatt acacttgccg tagatgccat
     9001 gggcggcgac caaggacttg ccgttaccgt acccggcgca accgcattcc tccaagcaca
     9061 ccccgatgtc cgcctgatta tgaccggcga cgaaacgcaa ctgcgccaag ccctgaccgc
     9121 ggcaggcgca ccgatggaac gcatcgacat ctgccatacc acccaagttg tcggcatgga
     9181 tgaatcccct cagtccgccc taaagaacaa aaaagactcc tccatgcgcg ttgccatcaa
     9241 ccaagttaaa gaaggcaaag cccaagccgc cgtatccgca ggcaacacgg gcgcgctcat
     9301 ggcaaccgca cgtttcgtcc tcaaaaccat tcccggcatc gaacgccccg ccatcgccaa
     9361 attccttcct tccgacaccg accacgttac cctcgccctc gacctcggcg caaacgtcga
     9421 ctgcacgccc gaacagctcg cccaatttgc cgttatcggc agcgaactcg tccacgcact
     9481 ccatcctcaa aaaggacagc cgcgtgtcgg gctggtcaac gtcggcacgg aagacatcaa
     9541 aggtacggac accgtcaaac aaacctacaa actgctgcaa aacagcaaac tcaactttat
     9601 cggcaacatc gaaagcaacg gcatcctgta cggcgaagca gatgtcgtcg tcgccgacgg
     9661 ctttgtcggc aacgtcatgc tcaaaaccat cgaaggcgcg gtcaaattca tgagcggagc
     9721 catccgccgc gaattccaaa gcaacctgtt caacaaactt gccgccgttg ccgccctacc
     9781 cgccctcaaa gggctgaaaa acaaactcga tccgcgcaaa ttcaacgggg ccatcctgct
     9841 cgggctgcgc ggcatcgtca tcaaaagcca cggcggcaca gacgaaaccg gtttccgcta
     9901 cgcactcgaa gaagcctacc acgaagccaa gtccgccggc ctttccaaaa tcgaacaagg
     9961 cgttgccgaa caactcgccg cactcgaaac tgccaaagcc gtccaaaacg aaaatgccga
    10021 cggtctgtaa cacacacgat gccgtctgaa cgcccccgcc cctttcagac ggcatccgcc
    10081 cgcaccaaac ctgcgggcgc ggacggcgat gcgcctgtcc ggcacttccc aaatacttcc
    10141 ctgtaaaata aggagtattt gaaaaatgaa gacattagaa aaacggatga aagctctaga
    10201 caaacagatg atgaagttcg gaaaatccct tgaaggcagg cttgatgccc gtctgattga
    10261 atccgcattg gattatattc attattcgga acgttttttg gcttttgaaa tcctgtgtac
    10321 ttatatcgaa gatttcgatg tccggctgac ggaacaagaa tcccgggaaa tttcttttat
    10381 caacaaggaa tttgagatag aaagcacgtc cgattaacca ataaagccaa tgggttgata
    10441 aacatgaaaa catcgacggt cgtttttggc ggatttttta tggcagacaa cggagagcga
    10501 atccaaatcc ccgttttgga aaatcctgac attagggaaa tcaatcactt ttttccgtat
    10561 caaattttga gaaaaaagcc ggcgtccttg ttttcagaat catccccgag ccggaatttg
    10621 gcgataccga attaactatc tattttgaaa aaggatatta tttgccaatg attcaaacaa
    10681 ttttagaaga cggggatatt gaagtcaaaa accttaaaac ggaaaattac agcgggaagg
    10741 caagggaaat cttgggagat tcctacccta ttgagcatat ctctaaaaat atttctacaa
    10801 ttcaaaatat aatttctgaa tttatgatac aaaaacaaac accggcacca ataatttgat
    10861 ggaaaaccaa caatcaaaat gccgtctgaa gcatgtttgg ttttcagacg gcatttcctt
    10921 cgcttaaaac agcgtatcgg caaccccgcc ctgcctgtcc acggcaatct gcatctgaaa
    10981 accatctgta tcccaaacca cacccccatc cctgtttccg tcatttgcac cctgtccgta
    11041 ttgggcaatc atcggttttt cgcttacaat agccgaatct gaaccaacta tccaaaaagg
    11101 ccgttcccat gcagtatgcc aaaatttccg gcacgggcag ctatcttccc gccaaccgcg
    11161 tcagcaatga cgaccttgcc caaaaagtgg atacctcgga cgagtggatt accgcgcgca
    11221 cgggcatcaa attccgccat attgcagccg aaaatgagaa aaccagcgat cttgccgccg
    11281 aagcagcccg ccgcgcactg gatgcagccg gattagacag cggcgaaatc gatttaatta
    11341 ttgtggcgac ggcaacgccg gatatgcagt ttccttctac cgccaccatc gtccaacaaa
    11401 aactgggcat caccaacggc tgccccgcgt ttgacgtaca ggcggtgtgt gcgggcttta
    11461 tgtacgcgct gaccacggca aacgcctaca tcaaaagcgg catggcaaaa aacgcgctgg
    11521 tcatcggcgc ggaaactttc agccgcatcg tcgattggaa cgaccgcaca acctgcgtat
    11581 tgttcggcga cggcgcgggc gcggtggttt tgagcgcggc ggacaaaccg ggcatcatcc
    11641 acagcaaact caaagccgac ggcaattatc tgaaactctt aaacgtcccc gggcaaatcg
    11701 cctgcggcaa agtttccggt tcgccgtaca tttcgatgga cggtcccggc gtgttcaagt
    11761 ttgccgtcaa aatgctgtcc aaaatcgccg atgacgttat cgaagaagcc ggttataccg
    11821 ccgctcaaat cgactggatc gtgccgcatc aggcaaaccg ccgcattatc gaatcgaccg
    11881 cgaaacattt aggtttgagt atggacaaag tcgtcctgac cgtccaagac cacggcaaca
    11941 catccgccgc atcgattccg ctggctttgg atacgggcat ccgcagcgga caaatcaaac
    12001 gcggtcaaaa cctgctgctc gaaggcatcg gcggcggttt cgcgtggggc gcggtgctgt
    12061 tgcaatattg aacccgatgc cgtctgaaac aggctttcag acggcatttc ccatatcatg
    12121 aagcggcagg ctttcttcaa actgatggcg tgtgcggcat ttctgtctgc cgtttcgctg
    12181 cgcctccccg tattgggcgc gtgttacgca atattgtccc tctatgcgtt tgcactttac
    12241 ggcatcgaca aacggcgtgc cgtgcgggga aaacgccgca ttcccgaaca ccgcctgctg
    12301 ctgcctgcct tgttcggcgg ttgggcgggc gcatacttgg gcagcaggat attcaggcat
    12361 aaaacggcga aaaagcgttt tgttgtgctg ttccgtctga ctgtttcagg taatgtcctg
    12421 gcgaccctca tcctgattta tagtggatta aatttaaacc agtacggcgt tgcctcgcct
    12481 tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
    12541 tatattattt cgttccgccc gaactttttg taaaactcgg gcagaatacc tgattatcca
    12601 accaaataaa ggaataccat gtcttttgcc ttcttttttc ccggacaagg ttcccaaagc
    12661 ctcggtatga tgaacggctt tgccgaacac gccatcgtca aaaacacatt tgccgaagcc
    12721 tccgccatat tggggcagga cttgtgggcg atgattaacg gtagcgatgc cgaaatcatc
    12781 ggtcaaaccg tcaacaccca gcccatcatg ctcgccgccg gcgttgccgt ttaccgcgcc
    12841 tatttggaag cgggcggcaa aacgcctgcc gccgttgccg gacacagcct cggcgaatat
    12901 accgcgcttg tcgccgccga cgcattggat tttgccgacg cggtcaaact cgtgcgcctg
    12961 cgcgccgaac tgatgcagtc tgccgtaccg caaggcgtgg gcgcaatggc ggcgattctc
    13021 ggcttggaag atgagcaggt aaaacaaatt tgcgccgaag ccgcccaagg cgaagtggtc
    13081 gaagccgtca acttcaactc acccggacaa atcgtgattg caggcaacgc cgccgccgtc
    13141 ggacgcgcca tgaccgccgc caaagaagcc agagccaaac gcgccctgcc gctgcccgtg
    13201 tccgtacctt cccattgcag cctgatgaaa cccgccgccg acaaacttgc cgaagccctg
    13261 aaaaccgttg aaatcaaaca gccgcaaatc cgcgtcatcc acaacgccga cgttgccgcc
    13321 tacgatgatg ccgacaaaat caaagacgcg ctcgtccgcc agctttacag ccccgtacgc
    13381 tggacggaaa ccgtcaacgc ccttgtttca gacggcattg ccgaatccgc cgaatgcgga
    13441 ccgggcaaag tgttggcggg cttggcaaaa cgcatcaaca aaaccgccgc gtgcagcgcg
    13501 ctgaccgatg ccggacagat tgccgccttt atcgaagcgc actgacttcg ttctgtaaaa
    13561 agcagcctac cctcttcagg ctgcttttca cgtccgaacg gcggcagccc cgtatttacg
    13621 ctataatcca tcccgaccaa accaccgaca gcggctgccg ttgcagttcc cgccctaccg
    13681 atatgataga aaaactgact ttcggactgt ttaaaaaaga agacgcgcgc agctttatgc
    13741 gcctgatggc atacgtccgc ccctacaaaa tccgcatcgt tgccgccctg attgccattt
    13801 tcggcgttgc cgccaccgaa agctaccttg ccgccttcat cgcccccctg attaaccacg
    13861 gcttttccgc acctgccgcg ccgcccgagc tgtctgccgc cgccggcatc atttccaccc
    13921 tgcaaaactg gcgcgaacag tttacctata tggtttgggg gacggaaaac aaaatctgga
    13981 ccgtcccgct cttcctcatc atcctcgtcg tcatccgtgg catctgccgc tttaccagca
    14041 cctatctgat gacttgggtc tccgtgatga ccatcagcaa aatccgcaaa gatatgtttg
    14101 ccaaaatgct gaccctttcc tcccgctacc atcaggaaac gccgtccggc accgtactga
    14161 tgaatatgct caacctgacc gaacagtcgg tcagtaacgc cagcgacatc ttcaccgtcc
    14221 tcacgcgcga cacgatgatt gttactggcc tgaccatcgt cctgctttac ctcaactggc
    14281 agctcagcat tatcgtcgtc ctgatgttcc ccctgctctc cctgctctcg cgctactacc
    14341 gcgaccgtct gaaacacgtc atttccgact cgcaaaaaag cataggcacg atgaacaacg
    14401 tgattgccga aacccatcag ggacaccgcg tcgtcaagct gttcaacggg caggcgcagg
    14461 cggcaaaccg gttcgacgcg atcaaccgca ccatcgtccg cctcagcaaa aaaatcacgc
    14521 aggcaacggc ggcacattcc ccgttcagcg aactgattgc ctcgatcgcc ctcgccgtcg
    14581 tcatcttcat cgccctgtgg caaagccaaa atggctacac caccatcggc gaatttatgg
    14641 cattcatcgt cgcgatgctg caaatgtacg cccccatcaa aagccttgcc aacatcagca
    14701 tccctatgca gacgatgttc ctcgccgccg acggcgtatg tgcatttctc gacaccccgc
    14761 ccgaacagga caaaggcacg ctcacaccgc agcgtgtcga agggcgcatc agcttccgca
    14821 acgtcgatgt cgaataccgt tcagacggca tcaaagccct cgacaacttc aatctcgaca
    14881 tcagacaagg cgaacgcgtc gccctggtcg gacgttccgg cagcggcaaa tccaccgtcg
    14941 tcaacctgct gccccgcttt gtcgaaccgt ctgccggcaa catctgcata gacggtatcg
    15001 acatcgccga catcaaactc gactgcctgc gcgcccaatt cgccctcgtc tcccaagacg
    15061 tattcctgtt tgacgacacc ctgtttgaaa acgtccgata cagccgtccc gacgcgggcg
    15121 aagccgaagt cctgtccgcc ctccaagccg ccaacctgca aagcctgatc gacgcatccc
    15181 cgctcggact gcaccagccc atcggatcga acggcagcaa cttgtccggc ggacagcggc
    15241 aacgcgtcgc cattgcccgc gccattttga aagacgcgcc gatattatta ttggacgaag
    15301 ccaccagcgc attggacaac gaatccgaac gcctcgtcca acaggcgctc gaacgcctga
    15361 tggaaaaccg caccggcatc atcgtcgccc accgcctgac caccatcgaa ggggccgacc
    15421 gcatcatcgt gatggacgac ggcaaaatca tcgagcaagg cacacacgaa caactgatgt
    15481 cccaaaacgg ctactacacg atgttacgca atatctcaaa caaagatgcc gccgtccgga
    15541 cggcataaac aaaatgccgt ctgaaatggt acaatcgccc cgaccctttc agacggcatc
    15601 atatccgccg acccatccga ttatcttcaa tcactgtaaa acccattatg acccaagaca
    15661 aaatcctcat cctcgacttc ggttctcaag ttacccagct gattgcccgc cgcgtgcgcg
    15721 aagcccacgt ttactgcgaa ctgcattcct tcgatatgcc tttggacgaa atcaaagcct
    15781 tcaaccccaa aggcatcatc ctctctggcg gccccaattc cgtttacgaa tccgactatc
    15841 aagccgatac cggtattttt gatttgggca ttccggtttt gggcatctgt tacggcatgc
    15901 agtttatggc gcaccacttg ggcggcgaag tgcagcccgg caaccagcgc gaattcggtt
    15961 atgcgcaagt taaaaccata gacagcgagc tgacacgcgg cattcaagat ggtgagccaa
    16021 acacactcga cgtatggatg agccacggcg acaaagtgtc caaactgccc gacggtttcg
    16081 ccgtcatcgg caacaccccg tcctgcccga ttgccatgat ggaaaacgcc gaaaaacaat
    16141 tctacggcat ccagttccac cccgaagtta cccacaccaa acaaggccgc gccctgttga
    16201 accgctttgt cttggatatt tgcggcgcac aaccgggctg gacgatgccg aactacatcg
    16261 aagaagccgt tgccaaaatc cgcgaacagg tcggcagcga cgaagtgatt ttaggtttgt
    16321 caggcggcgt agactcttcc gtagccgccg cgctgattca ccgcgccatc ggcgaccaac
    16381 tgacctgcgt gttcgtcgat cacggtttgt tgcgcctgaa cgaaggcaaa atggtgatgg
    16441 atatgttcgc ccgcaacttg ggcgtgaaag tgattcacgt cgatgccgaa gggcagttta
    16501 tggcgaaact cgccggcgtt accgaccctg agaaaaaacg caaaatcatc ggcgcggaat
    16561 ttatcgaagt atttgatgcc gaagagaaaa aactcaccaa cgccaaatgg ctggcgcaag
    16621 gcacgattta ccccgacgta atcgaatccg caggcgcgaa aaccaaaaaa gcccacgcca
    16681 tcaaatcgca ccacaacgtc ggcggtctgc ccgaaaatat gaagctcaag ctgcttgagc
    16741 cgttgcgcga cttgtttaaa gacgaagtgc gcgaactggg cgttgctttg ggtctgccgc
    16801 gcgaaatggt gtaccgccat ccgttccccg gccccggttt gggcgtgcgc atcttgggcg
    16861 aagtgaaaaa agaatacgcc gacttattgc gtcaagcaga cgatattttc atccaagaat
    16921 tacgcaacac caccgacgaa aacggcacat cttggtacga cctgaccagc caagccttcg
    16981 ccgtgttcct gcctgtgaaa tccgtcggcg taatgggcga cggacgcact tacgactacg
    17041 tcgtcgcact gcgcgcagtc atcaccagcg acttcatgac cgcacactgg gcagaactgc
    17101 cttactcact gctcggacgc gtgtccaacc gcatcatcaa cgaagtcaaa ggcatcaacc
    17161 gcgtggtgta cgatgtcagc ggcaaaccgc ctgccaccat cgagtgggaa taaccagcaa
    17221 acagggctgc accgtccggc gcagtctttc gattatcgga aaaaaggaaa aaatatgagc
    17281 acacaagatt taagcagcaa aatcgctttg gtaacaggcg cctcgcgcgg tatcggtgca
    17341 gcaattgccg acacactggc ggcagccggt gccaaagtca tcggtacggc gaccagtgag
    17401 agcggtgcgg cggcgattag cgagcgattg gcgcaatggg gcggcgaagg ccgcgtatta
    17461 aattccgccg aacccgaaac cgtcgaaaac ctgattgccg acatcgaaaa aacgttcggc
    17521 aaactcgata ttctggtcaa caacgccggc atcacccgcg acaacctcct gatgcgtatg
    17581 aaagaagaag agtgggacga catcatgcag gtcaacctca aatccgtctt tcgcgcctcc
    17641 aaagccgtct tgcgcggcat gatgaagcag cgcgccggcc gtatcatcaa catcacatcc
    17701 gtcgtcggcg tgatgggcaa tgccggtcaa accaactatg ccgcggcaaa agcaggctta
    17761 atcggtttct ccaaatccat ggcgcgcgaa gtcggcagcc ggggcattac cgtcaactgc
    17821 gtcgcccccg gctttatcga taccgacatg acccgcgccc tgccggaaga aacccgccaa
    17881 acctttaccg cccaaaccgc cttgggcaga ttcggcgacg cgcaagacat cgccgatgcg
    17941 gttctgttcc tcgcttccga ccaagcaaaa tacatcaccg gtcaaacgat gcacgtcaac
    18001 ggcggcatgt tgatgccctg acggacaggc tttttaacca tgccgtctga aaaggtttca
    18061 gacggcattt gcattcccaa acaaaatgaa cacacaccac accctgccct gcccctgcgg
    18121 atcaggcaca agctgagacc tttgcaaaac tcctttccct cccgacagcc gaaacccaaa
    18181 cacaggtttt cgtctatttt cgccccaaat atctcctaat tttacccaaa taccccctta
    18241 atcctccccg gatacccgat aatcaggcat ccgggctgcc ttttaggcgg cagcgggcgc
    18301 acttagcctg ttggcggctt tcaacaggtt caaacacatc gccttcagat ggctttgcgc
    18361 actcacttaa tcagtccgaa ataggctgcc cgggcgtagc ggaatttgcg
//
LOCUS       DAWFJR010000034        18244 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWFJR010000034 DAWFJR010000000
VERSION     DAWFJR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18244
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.034"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(150..1082)
                     /gene="cysK"
                     /locus_tag="WGR04_001074"
     CDS             complement(150..1082)
                     /gene="cysK"
                     /locus_tag="WGR04_001074"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ0794641.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAMAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1276..1881)
                     /locus_tag="WGR04_001075"
     CDS             complement(1276..1881)
                     /locus_tag="WGR04_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794642.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRVGMLQWFWASIVLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADSEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(1878..2729)
                     /gene="dapF"
                     /locus_tag="WGR04_001076"
     CDS             complement(1878..2729)
                     /gene="dapF"
                     /locus_tag="WGR04_001076"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ0794643.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQVVGRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            3176..3844
                     /locus_tag="WGR04_001077"
     CDS             3176..3844
                     /locus_tag="WGR04_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0794644.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(3917..6037)
                     /gene="pnp"
                     /locus_tag="WGR04_001078"
     CDS             complement(3917..6037)
                     /gene="pnp"
                     /locus_tag="WGR04_001078"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ0794645.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNPELWDWKAPETNEELVAKVREIAGETIKEAFKIRQKQARSAKLDEAWSA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RVGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRAITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6274..7137)
                     /locus_tag="WGR04_001079"
     CDS             complement(6274..7137)
                     /locus_tag="WGR04_001079"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ0794646.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGQTVYDVLPENEAKVLEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECERSIGKELAAEVRAKAIQLYTEAAEYAKSRGIIICDTKFEFGLDENGRLTLMDE
                     VLTPDSSRFWPADQYEVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7336..8199
                     /gene="rfbD"
                     /locus_tag="WGR04_001080"
     CDS             7336..8199
                     /gene="rfbD"
                     /locus_tag="WGR04_001080"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ0794647.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     VFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSATIIRLLQHSNPVRGIYHYAGGKSVSWYE
                     FVQHIFQAASQQQTSFPIPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
     gene            complement(8674..8907)
                     /locus_tag="WGR04_001081"
     CDS             complement(8674..8907)
                     /locus_tag="WGR04_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ0794648.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(9073..9273)
                     /locus_tag="WGR04_001082"
     CDS             complement(9073..9273)
                     /locus_tag="WGR04_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ0794649.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(9445..10320)
                     /gene="xerD"
                     /locus_tag="WGR04_001083"
     CDS             complement(9445..10320)
                     /gene="xerD"
                     /locus_tag="WGR04_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ0794650.1"
                     /translation="MEEGLIDRLLETLWLDRRLNQNTLNGYRRDLEKIARRLSQSGRT
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLATPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYAGRAGIGHISPHSLRHAFATHLVQHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(10372..10812)
                     /locus_tag="WGR04_001084"
     CDS             complement(10372..10812)
                     /locus_tag="WGR04_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ0794651.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            11133..12071
                     /locus_tag="WGR04_001085"
     CDS             11133..12071
                     /locus_tag="WGR04_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ0794652.1"
                     /translation="MSIHTLKHLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYSSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            12200..12493
                     /gene="hfq"
                     /locus_tag="WGR04_001086"
     CDS             12200..12493
                     /gene="hfq"
                     /locus_tag="WGR04_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ0794653.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            12578..13144
                     /locus_tag="WGR04_001087"
     CDS             12578..13144
                     /locus_tag="WGR04_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0794654.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTRTETSIAALSATLSLLKENGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLSYGFTNRKNNPPYLLAFEKLRQK"
     gene            13198..13572
                     /locus_tag="WGR04_001088"
     CDS             13198..13572
                     /locus_tag="WGR04_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ0794655.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRVFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            13582..14076
                     /gene="folK"
                     /locus_tag="WGR04_001089"
     CDS             13582..14076
                     /gene="folK"
                     /locus_tag="WGR04_001089"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ0794656.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFINAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKHGKVAELSKRLGNQGIRL
                     LPDK"
     gene            complement(14203..14682)
                     /locus_tag="WGR04_001090"
     CDS             complement(14203..14682)
                     /locus_tag="WGR04_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ0794657.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVDEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDCKDNAYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVFQI"
     gene            complement(14901..15638)
                     /gene="ubiE"
                     /locus_tag="WGR04_001091"
     CDS             complement(14901..15638)
                     /gene="ubiE"
                     /locus_tag="WGR04_001091"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ0794658.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGRLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(15681..16049)
                     /locus_tag="WGR04_001092"
     CDS             complement(15681..16049)
                     /locus_tag="WGR04_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ0794659.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            16175..17794
                     /gene="mnmC"
                     /locus_tag="WGR04_001093"
     CDS             16175..17794
                     /gene="mnmC"
                     /locus_tag="WGR04_001093"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ0794660.1"
                     /translation="MDNLVWDGIPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASYGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHNTEQTELLLTGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLNTPYAEPLCGLFWQHGVW
                     LNPPAFVRALLRHPLIGLHEDTPLTGISHDGEKWIASTPNGIFSATHIVYCTGAHSPC
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALAESLFAANPNPQKYQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAVAAEILG
                     LPHPLSKRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(17791..17940)
                     /locus_tag="WGR04_001094"
     CDS             complement(17791..17940)
                     /locus_tag="WGR04_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794661.1"
                     /translation="MDSKGNLLGIPYSLFWEENVMGVFDILPSGLVCLFEYDFLWSGR
                     HAAKA"
BASE COUNT         4659 a         5402 c         3904 g         4279 t
ORIGIN      
        1 aatatttccc tcaagcgatg tggcggcacc aacacctgat taaaacacaa ccggcgtttt
       61 cggcatatac gcaaaaacgc atttcccagc acggcgttac accgataccg aaaaatgcgt
      121 ctgaatgttt cgacaatccg attaaagcat tatgccaaat ctgcaaaaag tggcgtagag
      181 agatagcgtt cgccataaga aggcagcagc acgactatca gcttgccttc gttttcaggc
      241 tgtttggcaa gctgcaacgc actccaaacc gccgcaccgg aagaaatacc caccaaaatg
      301 ccttcttttt ccgccattgc gcgggcggtt tcaaaagccg cttcgttcga cactttggta
      361 atgctgtcgt aaattttggt attcaaaacg gtcggaataa agcctgcgcc gatgccttga
      421 atcgggtgcg ggcctttttc gccgccgctc aatacggggg aagcttcagg ctcgacggca
      481 accactttaa cttcgggttt gtattttttc aacacttcgc ccacgccggt aatcgtaccg
      541 cccgtgccga cgccggcaac gaagacatcg actttgccgt ccgtatcccg ccaaatttcc
      601 tcggcggttg ttttgcggtg gacttcgggg tttgcctcat tgtcgaactg gcgcggcata
      661 aaataagtgt cgggatgcgc gtccaccaag gatttcgctt tggcaatcgc gcccgccata
      721 ccttcggcgg caggggtcag aatcagctcc gcaccaaacg cgcgcaacag cattttgcgc
      781 tccttactca tgctttccgg catggtaatc gccagcttgt agccgcgtgc ggcacatacc
      841 attgccaaac cgacacccgt attgccgctg gttgcttcga caatgacggt gtttttgttg
      901 attttgcccg ctttttcggc accctcgatc attgcttcgg caatgcggtc tttgacgctg
      961 ctgcctggat tgaaaaattc cagtttcacg gcaacctccg ccttcaaacc ttcggtcaga
     1021 cggttcagtt tgaccaaagg cgtgttgccg attagttcgg tgatgctgtt tgcaattttc
     1081 atcttctgct cctgaataat aaggtttcga cagtaaggcg caatccccat aggcatcctg
     1141 tccgattgca ggggatttgc gccgaaacat aaacaaattt cggatatggc tttatcctaa
     1201 acctatgccg cctaaaaaac caatatccaa aatttctttc tttataactt tttgttccga
     1261 tacggccgca ccgtattatc ttccaatttc tgaaagcagc agcaccagta ccgagacctg
     1321 catcaacgct gccgtcaacc cgccgagtcg cagctgcgag ccgccgaacg ttgacggcgg
     1381 ctgccggtct ataccgcagc ggtggacata ggcaaccgcc atcagcacaa actgcaacac
     1441 gaaccaataa accggcttca gcgcataaag tttgagcagt gccgcaaaca caaacacttg
     1501 aaccgttccc gaaaagcaat aatgcaccaa cgcacatatt ccgacaaaag caagcgtcat
     1561 cgatacatta ctgacggcaa aaagcccgag cagcggatgt tcggagtctg cctgccatcc
     1621 gtttccatct gttttccggt tccaatgccc gatgaaaaaa aatatgctgc ccaaagtcag
     1681 gtaaaaatgg gggatgaaca agggcgcggc gcgggtcatt ccccatatgc cgggcatcag
     1741 ctttgccccc aaaaccgata tgcccagcca cagcacaata ctcgcccaaa accattgcaa
     1801 cattcctacg cggaatatca gaaacagaaa aaccgccgca tacagtaagg cagacaaaaa
     1861 atccaaatgc agcaaaatca tgaatacgcc aactcgcctt caaacaccgt ttccgcaggg
     1921 ccggtcatca tcacatcgcc gccgcaggcc cattcgatat ataaagttcc gcccggcaaa
     1981 atcacctcta ccgttttccc ttcatccaac aaccccagac ggatacccgc caccacagcc
     2041 gcacacgcgc ccgtaccgca cgcttgggtt tcgcccacgc cgcgctcgaa cacgcgcaaa
     2101 cgaatcgcgg ttcggccgac aacctgcata aagccgacat tgacgcgttc gggaaactgc
     2161 ctgtgcggtt cgataagcga accggtttcg cgcaccggcg cgcattccac gtcatcgacc
     2221 acaatcaccg catggggatt gcccatattg acgcagctga caggctgaat gccggattcg
     2281 agatgcaccc cgtaaataca ggcatcatcc ccctcgcccg attcggggac aaacggtatt
     2341 tcagacggca taaacttcgg tttgcccata ttgaccgtaa ccataccgtt atcggacaat
     2401 ttcggaaaaa taacgccatt tgccgtttca acacaaattt ctttcttatc ggtcaaaccc
     2461 ttgtctgcaa caaaacgggc aaaacaacgc gctccgttgc cgcattgccc gacctcgctg
     2521 ccgtcggcat tgaaaatacg gtaacggaaa tccacgcctt cggtttccga acgcccgacc
     2581 accaaaagct ggtcgaagcc cacgccccgg aaacggtccg cccatgccgc aatcggcgcg
     2641 tcctcggggg taaaatcctg actgaccgcg tcaatcacca taaaatcgtt gcccaaaccg
     2701 tgcattttgg taaatttcaa agtgttcata ttgttccgaa atcccgtatc gtctgttgct
     2761 tccgaatccg aaatcccccg caccgaaggg aaatgcttcc atctcccgcc gcctgaggat
     2821 taaaatcagc cattatactg tttgttaccc cttacaagaa acaccatgtt ccgcatatta
     2881 ctgttgcttt tcccttgtgc aatccttgcc gcctgcaccc gaaacatacc ggcaaacccg
     2941 gccgtttcaa attgcgcgca tcttcaaaac cgcaccatcc tgtattttga cgcacacggc
     3001 aaacccgctg acggcgcagt aaagggcgga ttttacaaaa ttatcgaaat acagggtgca
     3061 aactgcatcc ttattcaaga ctttaacagc acgggcaatg ccctcggcgg attctatgtg
     3121 ccggactgcc agaaatcatc cctttcaccg aaaaatatct gacttcaaac tgcctatgaa
     3181 ccacgacatc actttcctca ccctgttcct actcggtttc ttcggcggaa cgcactgcat
     3241 cggtatgtgc ggcggattaa gcagcgcgtt tgcgctccaa ctccccccgc atatcaaccg
     3301 cttttggctg atcctgctgc ttaacacagg acgggtaagc agctatacgg caatcggcct
     3361 gatactcgga ttaatcggac aggtcggcgt ttcactcgac caaacccgcg tcctgcagaa
     3421 tattttatac acggccgcca acctcctgct gctcttttta ggcttatact tgagcggtat
     3481 ttcttccttg gcggcaaaaa tcgagaaaat cggcaaaccg atatggcgga acctgaaccc
     3541 gatactcaac cggctgttac ccataaaatc catacccgcc tgccttgcgg tcggaatatt
     3601 atggggctgg ctgccgtgcg gactggttta cagcgcgtcg ctttacgcgc tgggaagcgg
     3661 tagtgcggca acgggcgggt tatatatgct tgcctttgca ctgggtacgc tgcccaatct
     3721 tttagcaatc ggcatttttt ccctgcaact gaaaaaaatc atgcaaaacc gatatatccg
     3781 cctgtgtacg ggattatccg tatcattatg ggcattatgg aaacttgccg tcctgtggct
     3841 gtaagcccca ttcattcccg atacccgtaa aaaataccgt ctgaaacctg ttcagacggc
     3901 actttcaccc taagcgttac tcggcggcat tttcctcacg ggcaggcgcg tccagcaggg
     3961 ctttgatgga cagacggaca cggcctctgt cgtccacttc caatgctttc acgttcacca
     4021 cctgaccgac ttgcaggtag tcgccgacat tgcgtacgcg ctcgtgggcg atttggctga
     4081 tgtgtaccaa accgtctttg cccggcatca cactgacaat cgcgccgaca ttgttgtcga
     4141 ggattttcac cacagtgcct tcgtacactt tgcccacttc cacttcggca gtaatctgct
     4201 cgatgcgttt tttcgccgca tcgccggctt cttgagtggt tgcggcaatg gtaatcgtac
     4261 cgtcttcggc aatattgatt tccgtaccgg tttcagcggt aatcgcacgg atggtttcac
     4321 cgcccttacc gataacttcg cggattttgt cttggttgat tttcatcgtg aacaagcgtg
     4381 gcgcgtgtgc ggacagctct tgcgggcccg caacggcggc tttcatctga tccaagatgt
     4441 gcagacgcgc ttctttggcc tgtgccaaag cgatttgcat gatttctttg gtaatacctt
     4501 ggattttgat gtccatttgc agcgcggtta caccttcggt cgtaccggct actttaaagt
     4561 ccatatcgcc caagtggtct tcgtcgccca agatgtcggt caggacggca aatttgttgc
     4621 cttccagaat cagacccata gcgataccgg caacgtgtgc tttcaaaggc acgccggcgg
     4681 acagcaggct caggcagccg ccgcagacgg aagccataga ggaagagccg ttggattcgg
     4741 taatttcgga gaccacgcgc atggtgtagc tgaaatcttc aggtttcggc aatacggcca
     4801 acaatgcacg tttagccaaa cggccgtgac cgatttcacg acgttttggc gcacctacgc
     4861 gacccacttc gccggtagag tatggaggaa agttgtagtg cagcatgaag cggtcggtgt
     4921 attcaccgga cagcgcatca atgatttgct cgtcgcgtga agtacccaaa gttgcaaccg
     4981 ccaaagcttg agtttcgcca cgggtaaaca atgcagaacc gtgcgtgcgc ggcaatacgc
     5041 cggtttgaat gttcagcgga cgaacagtgc gggtgtcgcg accatcaata cgcggttggc
     5101 cgtctaaaat ttggctgcgg acaacatcgg cttccaagtg tttgaaaatg cctttgattt
     5161 cgttggctgc caaagtgtcg gtttcctcgg taatcaaggc ttcttttaca gcactccaag
     5221 cttcgtccaa tttggcagaa cgcgcttgtt tttgacggat tttgaatgct tctttaatgg
     5281 tttcaccggc aatttcgcgt acttttgcaa ccaattcttc attggtttca ggtgctttcc
     5341 aatcccaaag ttccggattg acttcgtcgg caaattcatt gattgcattg atggcaacct
     5401 gcatttgatc gtggccgtaa accaccgcgc ccagcatcac gtcttcgggc aggattttgg
     5461 cttcggattc caccatcaac acggcttttg aagtaccggc gaccaccaag tccaattgcg
     5521 atttcgccaa ttcggcttta gtcgggttca aaacgtacac gccgtttaca taaccgacgc
     5581 gtgccgcgcc gatcgggccg gcaaacggta cgccgctcaa caccagcgcg gcagatgcac
     5641 ccaacattgc aggaatatca gaatcgattt caggatcgac ggacacgacc atcgctacga
     5701 tttggatgtc gtggtagaaa ccttcaggga acagcggacg aatcggacgg tcgatcagac
     5761 ggctggtcag gatttctttt tcgctttgtt tgccttcgcg tttgaagaaa ccgccgggaa
     5821 ttttgcctgc ggcgtaagtg cgctcgaggt aatcgacagt cagagggaag aagtcttggc
     5881 cttctttcac ttctttgttg gtggtaacgg caaccaaaac cacggtgtcg cccatagaga
     5941 ctttaacggc agcggcggct tggcgggcaa tttcgccggt ttccaaagta acggtgtggt
     6001 tgccgtattg gaaagtttta acgtgtttgt cgaacattat tgttcctttc aaaataccgc
     6061 actgctaaaa cactaataat gcacactaaa atccgaatgt gcatagttag ggtttcagac
     6121 cgtgcggcag gttgtaaaca agctttcaga cgacatttcg gacgctgaaa gcaattgcgg
     6181 attataaagg caaccatcct taaaatccag tattgataca ataaaaaggt cgtctgaacc
     6241 atatttcttt cagacggcct tcaaacttaa aaatcaatcc tgagtcagca aagtcaatgc
     6301 ttcccgatac ttctcgacag ttttctgaat cacatcggca ggcactttcg gcgcaggggc
     6361 ttttttgttc caaccgcttt gttccagcca gtcgcggacg aattgtttgt caaaagacgg
     6421 cggattggtg ccgacttcat attgatccgc cggccagaag cggctcgaat cgggagtcaa
     6481 tacctcatcc atcagcgtca gcctaccgtt ctcatccaaa ccgaattcaa atttggtatc
     6541 gcaaatgatg atgccgcgcg atttagcata ttccgccgct tctgtataaa gctgaatcgc
     6601 tttggcgcgc acttccgccg ccaattcttt tccgatactg cgttcgcatt cttcaaagct
     6661 gatgttttca tcgtgatcgc cgactgcggc tttggttgag ggcgtaaaaa tcacttcagg
     6721 cagttgttgc gcttcctgca taccttcggg cagccgaata ccgcaaaccg agccggtttt
     6781 ttgataatct ttccaaccgc tgcctgccag ataaccgcgc acaatcgcct ctactttcac
     6841 cggagtgagc tttttagcca cgacggcgcg tttttctaaa actttggctt cgttttcagg
     6901 caaaacatcg taaaccgttt gaccggtaaa gtggttgggc ataatatgcg ccagtttttt
     6961 aaaccaaaaa ttggaaatct gcgtcagaat ctcccctttg ctcggaatcg ggtcgtccaa
     7021 aatcacatca aacgcggaca ggcggtcgga agcgaccatc agcatacgtt tatcgtcgat
     7081 ttcatataaa tcgcgcactt ttccagaata gatctttacc aaaccaatct cactcatttc
     7141 gccccccctg aaaatatctt gaaaataccg acccgacacc cgacaggttt gaatcgcaaa
     7201 ccgatattct agccgaagtc ggcgcaaaac aatacccatg gcacaaaaag ccaacccgtc
     7261 aaccgtcggc aaagttttgg cactataata ccgacagcaa gtcctacaat acacttttac
     7321 caaaggaaat acctcatgag aatcctattg acaggctcga aaagccaact ggcacgctgc
     7381 ctgcgcgacc gtcttccgga agactgggaa accattgcga cggattccgc atccctagac
     7441 attaccgatg ccgatgccgt ctgcaacatg gtcaaaagtt tccaacccga cgccattgtc
     7501 aacacggctg cctatactgc cgtcgacaaa gcggaaggcg atgcggcagc ggcatttgcc
     7561 gtcaatgctt ccgccgttta caaccttgcc ttggcagcac atcgcgccca tgcccgattc
     7621 atccacatct caaccgacta tgtatttgac ggtaaaggga aaagacccta tcaggaaagc
     7681 gactttacca atccttccaa tgtatacgga caatccaaaa ccgcaggcga gctgctcgca
     7741 ctgtctgcca atcccgacag ccttatcctg cggacttctt ggctgtttag cgaatacggg
     7801 gacaactttg tccgcacgat gctgaacctt gcgcgggaac gttccccgct gtccgccgtc
     7861 cacaaccaaa tcggctgccc gacctatgcc ggcgacttgt ccgccaccat catccgcctg
     7921 ttgcagcact ccaatcccgt tcgcggcatt taccactacg ccggcggcaa atccgtatct
     7981 tggtacgaat ttgtccaaca tattttccaa gcggcatcgc aacagcagac atccttcccc
     8041 attcccgaat tgactgccgt ttcagacaag gaatatccga ccgccgcccc caggcccgca
     8101 tacagcattt tggactgccg caaaatcgaa aacgacttcg gcatcaaacc gtcagactgg
     8161 caaaaagccc ttgcgcaggt cgtttccaag ctgctctgat gccgcccgcc tctccgtttc
     8221 cgccgtcaag caccgccttg gcggttttct tatatagtgg attaacaaaa accagtacgg
     8281 cgttgcctcg ccttgccgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt
     8341 tttgttaatc cactataaaa tctaccgatt acacaaacac atatcatctt tacacaatca
     8401 tgcttccatc aacagtaaaa catgatatga ttgccaacaa taacatctca caataaattt
     8461 tctaattttt attgaaaaaa tcaataaata agaattctcc caaccgacaa ataaaataaa
     8521 gaaagggtta atatgcaaca tcgaagaaga ctagcaattt accaagcatc caaacgtgct
     8581 tcctttaccg gcaggtcatc cgccccgcaa aaacgtaaag aacgttaatt tgaaaaaaat
     8641 gccgtctgaa gtcctgcttc agacggcatt tttttaccgt tcgagaaact gtttcaacct
     8701 gtcctcatcc aaaaaccagt gacagatttc ctcatctccc gccaacaaaa cgggaaccag
     8761 ctcattgtat ttttcttcca aaacaggatt ttcatccaca tcgaccactt ccagcccgaa
     8821 cccgtattca ttttgaaaag gtttgagtgc atcgcgcatt ttgtggcaca agctgcaata
     8881 ttcacgaaac atcaaggtca atttcatccg cgtttcctta tctgtcaatt tgcacacgcc
     8941 aaaaccttag acgcagcaga atcatggtct atttgggaaa aaacaatgtt ttcaaggaag
     9001 atgatactca agtcctgcca aaaacagcaa aaatgccgtc tgaacagttc agacggcatt
     9061 tccaaaaccg ttttacgctt gaacattgat acccgccgta accgtcggtt gcgcattgtg
     9121 cgccgtcaga aacgaagcgg caagctcccc acagcagccg cagcagctcg ctacgcccaa
     9181 ttgcgactgc aaatcgccca ttgtggtcgc gccggcggcg atggtttcct tgatttgatg
     9241 gtcggtaacg gcattgcaga tgcagacaaa cattttgtgc tccgtgtgtt ctcaaactat
     9301 cgtaccgata gcggctttta ttatcgtatg caaatataaa taaaaacggt tcgcattgca
     9361 aggtcggcat acacggtttg tcttggtaat tttttatcaa gttttcattt ccgattgatt
     9421 tcttattaaa ccgaagcaga agcctcagtt tcgggaatgg tgttccttca ctacaccctg
     9481 caaccataca ttggcaacat gggtgtaaat ctgcgtcgta ttcaaatcgg catgtcccaa
     9541 catatcctga accacgcgca aatccaatcc gtgctgcacc aagtgcgtgg caaaggcgtg
     9601 gcgcaggctg tgcgggctga tgtgcccgat gcccgcccta cctgcatatt ctttgacaat
     9661 catccatgcc aactgacgag aaatgcccgt ctttttctga ctgacaaaca atgcgtcgca
     9721 attcctgcct ttcagcagaa gtgggcgtgc ctccgtataa tagcgttcca cccaatacgc
     9781 cgactcctgc cccatcggga ccatcctctg cttatcgccc ttgcccagcg cggtaataca
     9841 gcccctgtcc aaatccacat tgccgaagtt cagcccgacc gcctcgctga cgcgcaagcc
     9901 ggtcgcgtac atcaattcga gcagtgcctt gtcccgcaaa ccgtgtggcg tatcagtatc
     9961 cggagtggca agcagtcggg aaatctgctg ctcggtgatc agggtcggaa tattcttgtc
    10021 gattttgggc ggtttcagca aacgggtggg attgtccgtc cttatgcctt cacgctccat
    10081 ccatatatac aggcgtttgc acgccgataa tgcgcgcgcc tgcgaactcc gttgctctcc
    10141 gtcaacataa accgccgccg ccaaatccgc ttcgtccgca tccttcagcg ttctgcccga
    10201 ttgggacagg cggcgggcga ttttttccaa atcgcgccgg taaccgttta aagtattctg
    10261 attgagccgc ctgtccaacc acagcgtttc aagcagcctg tcgatcaaac cttcttccat
    10321 accgttccaa acaaatgccg tctgaatctt cttcaggcgg catggttcac attatcggga
    10381 aagcgtttcc aacacttcct gtgcatgacc cgccactttg actttccgcc attcatgggc
    10441 gatttctcca tccttattca agacgaacgt actgcgctcg atacctaacg actctttccc
    10501 gtacagtttc ttcaatttga tgacatcaaa caggcggcac actgtttcat ccttgtcgct
    10561 caacagctcg aaccggaaac cctgcttggc gcaaaaattc tgatgcgcct ttacgccgtc
    10621 gcgggaaata ccgaccacgg tataacccaa tgcctcaaac tgttccaaac gcgcattgaa
    10681 atccaagcct tccgtcgtac agccaggcgt actgtctttc ggataaaaat acacgaccaa
    10741 aggcagatgt tctgccgaat gaaaatccgc accgctgctc gaaggcaggg taaattcata
    10801 tttcacatcc atagtcctac tcccgatatt cccattattc aaaacggcac gcagacgcac
    10861 cgccgcaatt gccaaaccaa ccccgattct accgccccaa aggacaagga ttcaaccgcc
    10921 ggaaacatcc aaaccgacac acgacggcat gaaaaatatc catgtcaaac cacaaaatat
    10981 gttccgattt aaaaacagaa tgttataaaa cccaatcccc aaaacacaac aagaccgccc
    11041 gcctacgggc agtctcctgt cagacgacat actttacaga tggctgtttt ttcaacaaaa
    11101 taacgccaat actcaaaaat atggaatcaa aaatgtccat ccatactctg aaacacctgc
    11161 cctcatcgct gctgctcggt ctctgccttt ccctgccgtc agcccacctt tttgccgaca
    11221 acgacatttt agggcaattt ttagaacaga acatgcttac ctcctccgat ccgatagaaa
    11281 tattcgccga aagcacgata caccccacca acacccaagc cattacaggc ggtctgattc
    11341 tctcctcaca gtctgccctg gtcgtcaaca acaaaaccgg acagatactg tatcagaaaa
    11401 acgccgacag gattatgccc atcgcctcca tttccaaact gatgagcgcg atggtcgttt
    11461 tggatgcaaa cttggacatg aacgaaaccg ttaccattac gcccgacgaa atcgaccgca
    11521 tcaaagggac cggcagccgt cttgccatag gtacggcact tacacgcaaa gaactgctgc
    11581 acctgagcct gatgagcagc gaaaaccgcg ccacccatgc attgggcaga acctaccccg
    11641 gcggcatggg cgcatttgtc gccgctatga accgcaaagc ccaaagcctg ggcatgtaca
    11701 gcagccgctt ttacgaaccg accggactca acttccaaaa cgtttccacc gccaaagacc
    11761 tgagccttat ggtcaacgcc gccgcccaat atccgcaaat ccgcaccaac tcgacttcca
    11821 actacgcctc ggtacagacc aaaaacgggc agcagaacta caaaaactcc aatgcgctgg
    11881 tcagggaagg catgtggaac atcgaattgc agaaaaccgg ctacatacgc gaagcaggca
    11941 ggtctatggt tgtcaaagcc aacattcaaa atcaacccgt taccatcgta ttgctgaact
    12001 cgcccacatc cgccacacgc gtcaacgacg cccgcaaaat cgaatcgtgg atgctgcagc
    12061 aacgctcctg acatacaaat gcccggcgga aaaccgccca aacacaaaaa aaattggtaa
    12121 tttttcctat tcaaggctac aattcgacac gcacagggcg tctgcccgat ttcacaatca
    12181 taatagcgga gttaaaaata tgacagctaa aggacaaatg ttgcaagatc ccttcctgaa
    12241 cgccctgcgt aaagagcatg ttccggtttc gatttactta gttaacggta tcaaattgca
    12301 aggtcaggtt gagtctttcg atcaatacgt tgttctcctg agaaacactt ccgtcaccca
    12361 aatggtttac aaacacgcca tttccaccat cgtaccggca cgctccgtca acctacaaca
    12421 tgaaaacaga ccccaagccg caccgacttc gaccctcgtc caagtggaaa ccgtccagca
    12481 gcctgccgaa taatccgcac gaagcatgac gtgtcatatc tttcaatacc ttaccggaca
    12541 acggtaaggt atttttattt tcagacagca tttaaaaatg ttattgcaaa acatccttcc
    12601 attcgcccat tgccttttgc ggaaggcact tcccgaaggt ggcaatgctt tggacggcac
    12661 cgccggcaac ggacacgaca cccttttcct cgcacaaacc gcaggcatcc gggggaaagt
    12721 gtgggcattc gacatccagc cgcaagccct gagccgcact cgatgccgtc tgaaagaagc
    12781 gagatacgag aatgtgcaac tcatcttaga cggacacgaa aacctgaaac aatatgtttc
    12841 agaaccacta aatgccgcca ttttcaattt cggctggctg cccggcgggg acaaaagcct
    12901 taccacccgc acggaaacca gcattgccgc cctttctgcc accttatccc tactgaaaga
    12961 aaacggtatg cttattgccg tcctctatcc gggacacgaa aacggcaaac aggaggcaga
    13021 agcaatcgaa caatgggcaa aaaacctgcc tcaagaacag tttgccgttt tgagttacgg
    13081 ctttaccaac cggaaaaaca acccgcccta tctgttggca tttgaaaagt tacgtcaaaa
    13141 ataaatgttt gcggtaaaat aagccgattc taacccttat tttgatacga gccgaccatg
    13201 caatatctga ttgtcaaata cagccatcaa atcttcgtta ccatcaccat tttggtattc
    13261 aacatccgtg ttttcctact ttggaaaaat ccagaaaagc ccttggtcgg cttttggaag
    13321 gcactgcccc accttaacga caccatgctg ctgtttacgg gattgtggct gatgaaaatt
    13381 acccatttct ccccgttcaa cgcgccttgg ctcggtacaa aaatcctgct tctgctcgcc
    13441 tatatcgcat tgggtatgat gatgatgcgc gcccgtccgc gttcgaccaa gttctacact
    13501 gtttacctgc tcgccatgtg ttgcgtcgcc tgcatcgttt accttgccaa aaccaaagtc
    13561 ctgcctttct gaaacaccgt tatgaacaac agacattttg ccgtcatcgc cctgggcagt
    13621 aatcttgaaa accctgccca acaggtacgc gccgcattgg acacgctgtc gtcccatcct
    13681 gacatccggc ttgaacaggc ttcctcactg tatatgaccg cacccgtcgg ttacgacaat
    13741 cagcccgatt tcatcaatgc cgtctgcacc gtttccacca ctctggacgg cattgccctg
    13801 cttgccgaac tcaaccgtat cgaagccgat ttcggacgcg aacgcagctt ccgcaacgcg
    13861 ccgcgcacat tggatttgga cattatcgac tttgacggca tctccagcga cgacccccga
    13921 ctcaccctgc cgcatccgcg cgcgcacgaa cgcagtttcg tcatacgccc tttggcagaa
    13981 atcctccctg attttatttt gggaaaacac ggaaaggttg ccgaattgtc aaaacggctg
    14041 ggcaatcaag gcatccgtct tttaccggat aagtaattcc gcacgcggat gccgtctgaa
    14101 tgccggtttc agacggcatt tttcctttgc cgccaacacg cgtgcaaaaa aatcgcccct
    14161 tggaaaaggg gcgcaaaagg aacacaaacc actaccaaaa ctttaaatct gaaacactgc
    14221 ctgccgcata ctgtatccga caggatataa agccctcact aaatcgtttc gagaaatcca
    14281 aacttcttca tcgccgacag aaaatctgcc tttctccggc accagctcca acaaaaacgg
    14341 ttgaaccgcc gtatgcagcc tgtccttaca ccgccccagc tttctggaca acgcggacag
    14401 ggcgcgtttg taggcattat ccttgcagtc aagctcccgc gcactgccga cccagctcaa
    14461 acgaaggttg cggctttcat cctcaatcag caaaatgcct gttttgactt cccccacctc
    14521 gggctcgcat gaaagcgcaa tataatattt gccgtcgatg ttgacatacg ccgcttcatc
    14581 cacctgacag ttgcagccgg aagtggcaag cagcgaataa acagaatccc gatgcagttt
    14641 caaaatatca agggaaatct gtttgaaaat actttccggc attttccctc ctttcgtctg
    14701 ctgcgttgtt tactggattg actgcattat acagacatct cccaatcttg caaatgataa
    14761 ttgttcctat tttttaaaaa gcttataaat tattgatata tatgtaaatt aatttttaag
    14821 atttaaaacc tttgtctcaa gaaacacaaa aatgccgtct gaaacgtgtt cagacggcat
    14881 tggctgcaca ggcagtccgt ttaaaatttc acgcccttat gcagcgcgac gatgcccgca
    14941 ctcatattgt gataatccac gctgtcaaag cctgcatcca gcatcatctg tttcaaagtt
    15001 tcctgatcgg ggtgcatacg gatggattcg gcaagatatt gataactctc cgcatctttg
    15061 gcaatcagcc tgcccatgac cggcagcagc ttgaacgaat agaaatcata cgcgccttcc
    15121 aaaggtttgt agattttgga aaactccaac accagcaacg tgccgcccgg tttcaaaaca
    15181 cggtacatct ctttcagcgc ggcatcttta tgcgtcatgt tccgcaagcc gaacgccacg
    15241 gaaaccaagt tgaaataatt gtcggggaaa ggcagttttt ccgcatcggc aagcgatacg
    15301 ggcaaaatca tgccttcgtt caacagacgg tcgcgcccga cggtcagcat agaggaatta
    15361 atatcggtca gccaaacctc gccttccttg ccgacccgtt tcgcccaacc gcgcgacaaa
    15421 tcgcccgtac cgcccgcaat gtccaacact ttatcgcctt ttttcaggtg cgccgtgttg
    15481 atggtgaaat gcttccacac cctgtgcagc cctgccgaca tcacatcgtt cataatgtcg
    15541 tagtttttgg cgacggagtg gaacacttcc gccacttttc cggctttctc atcttcgttg
    15601 accgtactga atccgaaatg cgttttttgt ccgcccatct tctgaccacc tccctgatgc
    15661 cgtcctccgg catcccaaac ttaacaatct gattgctttt caaaacgcga cgcaccagcc
    15721 gccgccaatt tgtccaaata ttcctgccac atcgcatcgt atccgtatgc cagtctgtgc
    15781 agatacgccc aatcgtaaag accgctgtcg tgcccgtccg aaaaactgat tttcagcgca
    15841 tactgtccga caggctgcaa atccgtgatt tggacatccg ccttgccggt ctgcaaaaca
    15901 tcctgtccca cgccgtgtcc gcgcacttcc gcactcggcg aatagacgcg taaaaattcg
    15961 gcaggcagat ttttaggttc gtccccataa gtcaaaacca acacggtacg gtttttcaac
    16021 aaacggattt caacaggaat attattcaaa aaacacctcg gttcggcata caatatccgg
    16081 caggcgcagg cgtgccttca aacggcgcgt attttaacag ctttatcccg ataccgatac
    16141 cgatattcaa acccacattt cagacggcat tgatatggac aatctcgtat gggacggcat
    16201 tcccgacatc cgcacactcg accaaaccat ccgcaaacac gcacacccgc tcaacctgat
    16261 tgtctgcctc cccgataatc agattcccga ttttcaaacc gcacaagatg cttcggacgc
    16321 ggaatgccgt ctgaagcacc gtttggatca ggcaacgcag tgcctccagt tcgacagcat
    16381 caacctcatc gaacacatcc tgcccgatgt ccgcttctgg ctggttcccc cttcacgcac
    16441 ccgccgcctg cacgaacact tccaccacat ttcctggcag accgaagcca tcccgcaaac
    16501 cgaaagtaag cccgacaaac cctggtttgc acttccacaa acatccgaac ggcaaaaacc
    16561 ggaacacatc ctcgttatcg gagcgggcat atccggcgcg gcaaccgccc acgccttagc
    16621 atcatacggc atttccgtta ccgtattgga agcccgaaaa gccgcccaag ccgccagcgg
    16681 caaccgccaa gggctgctct acgccaaaat ctcgccacac aataccgaac aaaccgaact
    16741 tttgctcacc ggctacggct acaccaaacg cctgctcgga cacatcctgc ccgaatccga
    16801 aacctggggc ggcaacggca tcatccacct caattacagc cgcaccgaac aacaacgcaa
    16861 tcacgaattg ggtttgcaaa aacaccataa ccacctctac cgcagcatca cgcaggcaga
    16921 agccgaaaaa atcgccggta tcccgctgaa cacgccctac gccgaaccat tatgcggact
    16981 gttttggcag cacggcgtat ggctcaatcc acccgcattc gtccgcgccc tcctccgcca
    17041 tccgctgatt ggactacacg aagacacacc gttaaccggt atttcccacg acggagaaaa
    17101 gtggattgca agcacgccaa acggcatctt ttctgccaca cacatcgtct actgcaccgg
    17161 cgcgcacagc ccctgcctgc ccgaaaccaa cctcgccgcc ctgcccctca ggcaaatacg
    17221 cggacaaacc ggcctcacac cgtccacccc gttttccgaa caactgcgtt gcgccgtttc
    17281 aggcgaaagc tacatcagcc cgtcgtggca cggactgcac tgctacggcg cgagttttat
    17341 tcccaacagc agccataccg gatggaacga agccgaagaa gcctcaaacc gccaagcatt
    17401 ggcacacctt aaccccgccc ttgccgaatc attgtttgcc gccaacccaa acccccaaaa
    17461 ataccaaggg cacgccgcca tacgctgcga cagccccgac caccttcccc tagtcggcgc
    17521 actcggcgac attgccgcca tgcggcagac ctacaccaaa ctcgcgctgg acaaaaacta
    17581 ccgcatcgac accccatgcc catacctgcc taatgcctac gtcaacaccg cccacggcac
    17641 acgcgggctt gccaccgccc ccatctgcgc cgccgccgtt gcagccgaaa tcctaggctt
    17701 gccccatccc ctctcaaaac gcctgcgcca cgccctacac cccaaccgca ccatcatccg
    17761 cgccatcgtc agaaggaagg atctaacccc ttaagccttt gccgcatgcc gtcccgacca
    17821 cagaaaatca tattcaaata aacataccaa tcctgacggt aaaatatcga aaacacccat
    17881 aacgttttct tcccaaaata aggagtaagg aatgcctaac aaattgcctt tgctgtccaa
    17941 gatgaatgtt ccgtaacatt cgaagacttc cttaaccctg ccagccgttt cggtttctgt
    18001 accccctgtt agataaggaa ggaatgaaaa agccctgttg attgtatgaa gtaaagggcg
    18061 ttaatttttg ttaatcttcc cttctcagga actcaatata taaggatgtt aatttttatt
    18121 tcccttctcg aggactcaat atagtggatt aacaaaaacc agtacggcgt tgcctcgcct
    18181 tgccgtacta tttgtactgt ctgcggcttc atcgccttgt cctgattttt gttaatccac
    18241 tata
//
LOCUS       DAWFJR010000035        18227 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWFJR010000035 DAWFJR010000000
VERSION     DAWFJR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18227
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.035"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(134..469)
                     /locus_tag="WGR04_001095"
     CDS             complement(134..469)
                     /locus_tag="WGR04_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0794662.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            598..1503
                     /gene="mtrA"
                     /locus_tag="WGR04_001096"
     CDS             598..1503
                     /gene="mtrA"
                     /locus_tag="WGR04_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ0794663.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1565..2053)
                     /locus_tag="WGR04_001097"
     CDS             complement(1565..2053)
                     /locus_tag="WGR04_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0794664.1"
                     /translation="MKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2200..3102
                     /locus_tag="WGR04_001098"
     CDS             2200..3102
                     /locus_tag="WGR04_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0794665.1"
                     /translation="MDTAKKDILGSGWMLVAAVCFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVELGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERIFVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHMLSWQSAVYLSGI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3143..4273)
                     /locus_tag="WGR04_001099"
     CDS             complement(3143..4273)
                     /locus_tag="WGR04_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0794666.1"
                     /translation="MTKHLPLAVLTALLLAACGGSDKPPAEKPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4478..7102
                     /gene="alaS"
                     /locus_tag="WGR04_001100"
     CDS             4478..7102
                     /gene="alaS"
                     /locus_tag="WGR04_001100"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ0794667.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYVSDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSMD
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGDAYPELKEKQVQIEEALKNEESRFAQTLETGMALLENALAKGGKTLDGEI
                     IFKLYDTYGFPYDLTADICRERNIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVDELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGEHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAILANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELAKAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKPLTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLVSVESWLCQKLS"
     gene            7164..7895
                     /locus_tag="WGR04_001101"
     CDS             7164..7895
                     /locus_tag="WGR04_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794668.1"
                     /translation="MNTKTELQKLLEEDISTLTETLICADALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYMAGGVYLGGEFISSIYH
                     SSQEFSGEPIIYAEPNIILCPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVHFDK
                     NYDKYKTWQVAIEKAANFLKLGNPYNEDKLSLSEEHDTILVVLPLEKGYEWNCLEIEV
                     LSAAQSLANIYCNKVRLIDGHVWKE"
     gene            8202..11014
                     /locus_tag="WGR04_001102"
                     /pseudo
     CDS             8202..11014
                     /locus_tag="WGR04_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
     gene            complement(11721..12404)
                     /locus_tag="WGR04_001103"
     CDS             complement(11721..12404)
                     /locus_tag="WGR04_001103"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ0794669.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSGQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVLEKFYL"
     gene            12701..15004
                     /gene="parC"
                     /locus_tag="WGR04_001104"
     CDS             12701..15004
                     /gene="parC"
                     /locus_tag="WGR04_001104"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ0794670.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLESNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKTDLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSSGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSG
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATNQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSELEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            15024..16599
                     /locus_tag="WGR04_001105"
                     /pseudo
     CDS             15024..16599
                     /locus_tag="WGR04_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            16592..17059
                     /locus_tag="WGR04_001106"
     CDS             16592..17059
                     /locus_tag="WGR04_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEZ0794671.1"
                     /translation="MTELQHPVLVVDNETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            17163..17915
                     /locus_tag="WGR04_001107"
     CDS             17163..17915
                     /locus_tag="WGR04_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0794672.1"
                     /translation="MTDILIDNTATETVRTLIQAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAARINLHFGNAAVQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMAYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
BASE COUNT         4576 a         5221 c         4408 g         4022 t
ORIGIN      
        1 tcacgcttac cccttgtttc tcaaatgccg tctgaaaaaa acggctgtat atgttgtctg
       61 caaaatatcg ggaagcataa cagacaaaat gccgtctgaa atattttcag acggcatttc
      121 aatccgattc ggattatttt tgcgtttcaa ccgcttccaa tgcacgcagg gcgtaagtgt
      181 aagcggcacc ggcattcagg gcgatggcgg ttgccaatgc gccggcgatt tcgctgtcgg
      241 tcgcaccagc tttggcggcg gcggcggcgt gaacactgat gcagctctca caacgtgtgg
      301 taatggcaac ggcgatggca atcagttcgc gcgttttggc atcaagtgct tcggcggcgg
      361 cggcctgttc caatgcgccg taggcctgca gcattttagg atgcgcctta cccagctcgc
      421 cgaacgattt tttaaccaat gcggtatgtt ctttccaatc tttaaacatt ttcttttcct
      481 ttctcttgcg tttaaccctg atacgcgctt gcgtatctgt tttcgatgtg cgtattattg
      541 caattattca gttgtgtttc tcgtttaatc atctcatttt atggttcaaa aagatttatg
      601 gacattctgg acaaactggt cgatttcgcc caattgacgg gcagtgtgga tgtgcagtgc
      661 cttttgggcg gacaatggtc ggtacggcat gaaaccttgc aacgcgaagg attggtacac
      721 attgttacat cgggcagcgg ctatctctgc atcgacggcg aaacttcccc gcgtccggtc
      781 agtacagggg atattgtatt tttcccgcgc ggcttgggtc atgtgttgag ccacgacgga
      841 aaatgcggag aaagtttaca accggatatg cggcagcacg gtgcgtttac ggtcaagcag
      901 tgcggcaacg gacaggatat gagcctgttt tgcgcccgtt tccgctacga cacccacgcc
      961 gatttgatga acgggctgcc tgaaaccgtt tttctgaaca ttgcccatcc gagtttacag
     1021 tatgtggttt caatgctgca actggaaagc aaaaaacctt tgacggggac ggtttccatg
     1081 gtcaacgcat tgtcgtccgt cctgctggtg cttatcctgc gcgcctatct cgaacaggat
     1141 aaggatgtcg aactctcggg cgtattgaaa ggttggcagg acaaacgttt gggacattta
     1201 atccaaaagg tgatagacaa accggaagac gaatggaatg tcgacaaaat ggtggcggct
     1261 gccaatatgt cgcgcgcgca actgatgcgc cgtttcaaaa gccgggtcgg actcagcccg
     1321 cacgcctttg tgaaccatat ccgcctgcaa aaaggcgcgt tgctgctgaa aaaaaacccg
     1381 gattcggttt tgtcggtcgc actgtcggta ggctttcagt cggaaacgca cttcggcaag
     1441 gcgttcaaac ggcaatatca cgtttcgccg ggtcaatacc ggaaagaagg cgggcaaaaa
     1501 taaatcgggg cttcaaacgc aaatgccgtc tgaaaaggct ttcatacagc atttgcgtac
     1561 cgcgtcattt caagggctgc atcttcatca cttccatcaa aaagttggta aatgcggggt
     1621 tgttgggttt gacatccata tttttccaac gctgctgcca gccgcgcaag gcattctgga
     1681 tatacagctt ggactgttcc gtattgattg cgccccgctg gctgtctatc gccgaacgca
     1741 ggtagatttc atacatactg tcatcgacgg cattgcgtcc gaccaggcgt tttctgaagt
     1801 tgttcagata ttgcgccgcc tgaaccttgg tcattttacc gatacccacc tgatagccca
     1861 agcgcgtcgc ttcatcgctg attttggcaa catccgtcca atgcgaagag gcaaggcgga
     1921 aaccttttgc aggtgcttcc gttttgacgg tattgatagg attcacgggg atttccgtca
     1981 atgtgggcac ataaatagac tggcagccgg aaagaactgc cgcaatggaa agagggataa
     2041 ggtatttttt catgccccca ttataatcaa gtttgccttg agaaaacaaa ttgttcggca
     2101 agaaaaataa aatttcggca tcagaagcag gcaaaaacac attccacaag ccttgccgca
     2161 aggtttacaa tccgaccgtc cttatcgcaa cgaccgttta tggataccgc aaaaaaagac
     2221 attttaggat cgggctggat gctggtggcg gcggtctgct tcaccattat gaacgtattg
     2281 attaaagagg catcggcaaa atttgccctc ggcagcggcg aattggtctt ttggcgcatg
     2341 ctgttttcaa ccgttgagct cggggctgcc gccgtattgc gtcgggacac cttccgcacg
     2401 ccccattgga aaaaccactt aaaccgcagt atggtcggga cgggggcgat gctgctgctg
     2461 ttctacgcgg taacgcatct gcctttggcc accggcgtta ccctgagtta cacctcgtcg
     2521 atttttttgg cggtattttc cttcctgatt ttgaaagaac ggattttcgt ttacacgcag
     2581 gcggtgctgc tccttggttt tgccggcgtg gtattgctgc ttaatccctc gttccgcagc
     2641 ggtcaggaaa cggcggcact cgccgggctg gcgggcggcg cgatgtccgg ctgggcgtat
     2701 ttgaaagtgc gcgaactgtc tttggcgggc gaacccggct ggcgcgtcgt gttttacctt
     2761 tccgtgacag gtgtggcgat gtcgtcggtt tgggcgacgc tgaccggctg gcatatgctg
     2821 tcttggcagt cggcggttta tctgtcgggc atcggcgtgt ccgcgctgat tgcccaactg
     2881 tcgatgacgc gcgcctacaa agtcggcgac aaattcacgg ttgcctcgct ttcctatatg
     2941 accgtcgttt tttccgccct gtctgccgca ttttttctgg gcgaagagct tttctggcag
     3001 gaaatactcg gtatgtgcat catcatcctc agcggtattt tgagcagcat ccgccccact
     3061 gccttcaaac agcggctgca atccctgttc cgccaaagat aaaaaatgcc gtccgaacat
     3121 ccttcagacg gcatatcggg ctttatttcc ccgccttcac atcctgccac tggcgcacca
     3181 taaacttcaa tgccgccggc tggataggca ccatgataaa gctgtttttc aaatcctcct
     3241 cggttgggaa aatcgtattg tcgtttttaa attcgtcttc catcagctca cgcgccggct
     3301 tgctcgaagg cgcataagta acgaaattgc cgtttttcgc cgacacttcc gggtcgagga
     3361 agtcgttgat gtatttgtgc gcgttggcga cgtttttcgc atctttcgga atcacgaaag
     3421 aatccaccca aatccccacg ccctctttcg gcatcatcac gcggattttt tccttgccgc
     3481 ccgcttcttc ggcacggcgt ttggcgatgt tcaaatcgcc gccgaaaccg attgttacgc
     3541 aggtatcgcc gcgcgccaaa tcatcgataa agccggacga agtaaagcgt ttgatattgg
     3601 ggcggttttt cttgagtagg gcggttgcct ccctgatgtc ttccgtattg ctgctgttcg
     3661 ggtttttacc caaatagttc aacaccatag gatagatttc cgccgcgctg tccaaatagc
     3721 tgatgccgca ttgcttgagt ttggacgtgt attcggggtc gaacaccaaa tcccactggt
     3781 tgtccggcag cttatccgta cccaaagcct ttttcacgcg ttcggtattg atggcgaagg
     3841 tatttgtccc ccaataaaac ggcacggcgt attcgtggcc gggatcgacc ccgtccatca
     3901 gcctcatcat ttcggggttg aggtgtttat aattgggaat cagcgactta tcgattttct
     3961 gatacgcacc tgccttaatc tgcctgccca caaacgcatt ggacggcgcg acaatgtcgt
     4021 aaccggactt gcctgtcagc accttgcttt ccagcgtttc atcgctgtcg tacacatcat
     4081 aagtaacctt gatgccgttt ttcttttcaa aatcggcaac ggtttccgga tcgacatatt
     4141 ccgaccagtt gtaaattttc aatacgtttt ggttttccgc cggtgccggt ttttcggcag
     4201 gcggtttgtc cgaaccgccg cacgctgcaa gcagcaaagc agtcaggacg gccaggggca
     4261 gatgtttggt cattatcatt ccttgcatat cgggttggag aaagcggcca ttatagccga
     4321 tattggcaac agggcttcag acggcattca aaatcccgcc acactcttcc gaaaaccgcc
     4381 gcttccatag ctagaaacag ggatttgcgg taagataccg ccgttcgttt tccctgcttt
     4441 taccatgaca agacatttga gagacattga aaaaattatg aaaacctccg aactgcgcca
     4501 aaaattccta aaattttttg aaaccaaagg ccacaccgtc gtccgctctt ccagcctcgt
     4561 gccgcacgac gacccgaccc tgctgtttac caacgcgggc atgaaccagt ttaaagacgt
     4621 attcttaggt ttcgacaaac gcccgtacag ccgcgccacc accgcgcaaa aatgcgtacg
     4681 cgcaggcggc aagcacaacg acttggaaaa cgtcggctac accgcccgcc accacacctt
     4741 ctttgaaatg atgggcaact tctccttcgg cgactacttc aaacgcgacg ccatccactt
     4801 cgcttgggaa tttctgactt cccccgaatg gctcaacatc cctaaagaca aactgttggc
     4861 gaccgtttac gcggaagacg acgaagccta caacatctgg ttgaacgaaa tcggtatgcc
     4921 gtccgagcgc atcgtccgca tcggcgacaa caaaggcgcg aaatacgtgt ccgacaactt
     4981 ctggcaaatg ggcgacaccg gcccttgcgg cccctgctcc gaaattttct acgaccacgg
     5041 cgaagaaatc tggggcggca ttcccggcag ccccgaagaa gacggcgacc gctggatcga
     5101 aatttggaac tgcgtattta tgcagttcaa ccgcgacgaa caaggcaata tgaatccgct
     5161 gcccaagccg tccgtcgata ccggtatggg cttggaacgt atggccgccg tcatgcagca
     5221 tgttcacagc aactacgaaa tcgacttgtt ccaagacctg ctcaaagccg ttgcccgcga
     5281 aaccggcgca gctttcagca tggatgaacc cagcctgaaa gtcatcgccg accacatccg
     5341 ctcctgctcg ttcttgattg cagacggcgt gttgccttcc aacgaaggcc gcggctacgt
     5401 attgcgccgc atcatccgcc gcgccgtgcg ccacggttac aaactgggtc aaagcaaacc
     5461 gttcttccac aaactggtgg ccgatttggt caaagagatg ggcgatgcct accctgaatt
     5521 gaaagaaaaa caagttcaaa tcgaagaagc gttgaaaaac gaagaaagcc gttttgccca
     5581 aacgctggaa accggtatgg ctttgttgga aaacgcgttg gctaaaggcg gcaaaacact
     5641 cgacggcgaa atcatcttca aactctacga tacttacggt ttcccatacg atttgaccgc
     5701 cgacatctgc cgcgaacgca atatcgaact ggacgaagca ggcttcgagc gcgaaatgga
     5761 agcccaacgc gcacgcgcac gcgccgccca aagcttcaaa gccaacgccc aactgcctta
     5821 tgacggtcaa gacaccgagt ttaaaggtta tagcgaacgc caaaccgaat ccaaagtcct
     5881 cgccctctac aaagacggcg agcaagtcga cgaattgaac gaaggcgaca gcggcgcagt
     5941 cgtcatcgac tttaccccgt tctatgcaga atccggcggc caagtcggcg acgtcggcta
     6001 tatcttctca ggcgaaaacc gctttgaagt acgcgatacc caaaaaatca aagcagccgt
     6061 attcggacaa ttcggcgtac aaacttcagg ccgtctgaaa gtcggcgaca gcgttaccgc
     6121 caaagtggac gacgaaatcc gcaatgccaa tatgcgcaac cacagcgcga cccacctgat
     6181 gcacaaagcc ctgcgcgacg tattgggcga acacgtcgaa caaaaaggct ctttggttac
     6241 cgccgaatcc acccgtttcg acatttccca tccccaagcg gtcactgccg aagaaatcgc
     6301 cgaagtcgaa cgccgcgtca acgaagccat tttggcgaac gttgccgtca acgccgccat
     6361 tatgagtatg gaggacgcgc aaaaaaccgg cgcgatgatg cttttcggcg aaaaatacgg
     6421 cgacgaagta cgcgtgctgc aaatgggcgg tttctctacc gaattgtgcg gcggcacgca
     6481 cgtttcacgc accggcgaca tcggtctttt caaaatcatc agcgaaggcg gtattgccgc
     6541 aggcgtgcgc cgtatcgaag ccatcaccgg cctgaacgca ctcaaatggg cgcaagagca
     6601 agagcgtttg gtgaaagaca tcattgctga aaccaaagcc caaaccgaaa aagacgtact
     6661 ggcgaaaatc caagcaggcg cggcacacgc caaagccttg gaaaaagagt tggcaaaagc
     6721 caaagccgaa ctcgccgtcc acgcaggcgc aaaactcttg gacgacgcaa aagacttggg
     6781 cgcagccaaa ctcgttgctg cccaaatcga agccgacgca gccgccctgc gcgaaatcgt
     6841 taccgattta accggtaaat ccgacaacgc cgtgattctt ttagcggcag taaacgacgg
     6901 caaagtctcc ctgtgcgccg gcgtatccaa accgttgacc ggcaaagtga aagcaggcga
     6961 tctggttaaa tttgcagccg aacaagtcgg cggcaaagga ggcggccgtc ccgacttggc
     7021 gcaggccggc ggcacggatg ccggcaaatt gcccgaaatg ctggtaagcg tggaaagctg
     7081 gctttgtcag aaattaagtt aaatttaaat cttaataagc catttgagtc ttgttcaaat
     7141 ggcttatatt ttggattgtt agaatgaata ctaaaacaga gttacaaaaa cttttagaag
     7201 aggatatatc aactttaaca gaaacactaa tttgtgcaga tgctcttcct ccaagatatg
     7261 tacgtagcat tgcaactcct attgtccgtc gatggttaat tgacaaacaa ttaaatattc
     7321 tcgcaaaaga aataggttta actatagaat tgcccatcct tgatacaagt ttggtatttg
     7381 aaaaattgtc aaccttagag aataaagtta atttttatat ggctggtgga gtttatttag
     7441 gcggagaatt tattagctct atatatcatt catcacaaga attttctggt gaaccaataa
     7501 tctatgctga accaaatata attttatgtc ctgctgaaaa atttttaact ttaaaacgag
     7561 ttttccataa tggaaatatt tttaatatga atcagataat tactttttta tccaataaac
     7621 aaggaggagt acattttgat aaaaattacg ataaatataa aacttggcaa gttgctatag
     7681 aaaaagcagc aaatttttta aaactcggta atccatataa tgaagataaa ttatctctta
     7741 gtgaagaaca tgatactatt ttagtagttt taccattaga aaaaggatat gaatggaatt
     7801 gtcttgagat agaagtacta tccgctgctc aatctctagc aaatatctat tgcaacaaag
     7861 ttcgcctcat agatggtcat gtttggaagg aataaaagaa aaagcatagt tggtaatttt
     7921 tagttacgta gggtgggctt cagcccaccg ttttttcatt tttgattgtt gattggtggg
     7981 ctaaagccca ccctacggtt actaaaatcc cgtcattacc tcgcaggcgg gaatccggtt
     8041 tgttcggttt cggtttttta gggacagtgc cgcaacatta aatttccaga ttcccacttt
     8101 cgcacaaata acagcgcaaa tagggcaaag ataaggatat gggttttttt ctttgaattt
     8161 actatacaat tcgcccgtcc ccgttataga aacccaagcc gatgaaaacc ctgctccaac
     8221 tccaaaccgc cgcacaaaac ttcgccgcct actacaaaga ccaaaccgac gaacgccgcg
     8281 agaaagacac cttctggaac gaatttttcg ccattttcgg catcgaccgc aaaaacgtcg
     8341 cccacttcga ataccccgtc aaagaccctg ccgacaacac ccaattcgtc gatatatttt
     8401 gggagggcat cttccttgcc gaacacaaat ccgccaacaa aaacctgacc aaggccaaag
     8461 agcaggcgga gcgttatttg caggaaatcg ggcgcaccaa gccctctgcg ctgcccgaat
     8521 attacgccgt cagcgatttt gcccatttcc acctttatcg ccgcgtacct gaagaaggcg
     8581 tggaaaacca atggcagttc cctttggaag cattgcccga atacatcacg cgcggcgttt
     8641 tcgacttcat gttcggcatc gaagccaaag tccgccaaat tcaagaagaa gccgatattc
     8701 aagcggcggc ggccatcggc aggctgcacg acgcgctcaa agaagaaggc atttacgaag
     8761 aacacgagct gcgcctcttc atcacgcgcc tgcttttcct cttttttgcc gacgacagcg
     8821 ccgttttcca gcgcaactat ctctttcaag actttttaga aagctgcaaa gaaaccgaca
     8881 cgctcggcga caagctcaac caactctttg aattcctcaa cacacccgac caaaagcgca
     8941 gcaagaccca aagcgaaaaa tttaaagggt tcgaatacgt caacggcggc ctgtttaaag
     9001 aacgcctgcg caccttcgac ttcaccgcca agcagcaccg cgccttaatc gactgcggca
     9061 atttcgactg gcgcaacatc agccccgaaa tcttcggcac gctcttccaa tccgtcatgg
     9121 acgcgcaaga gcggcgcgaa gcgggcgcac actacaccga agccgccaac atcgataaag
     9181 tcatcaacgg ctttttttag aaaacctgcg cgccgaattt gaagccgtca aagccctcaa
     9241 acgcgacaaa gccaaaaaac tcgccgcctt ctaccaaaaa atccaaaacc tgcaattcct
     9301 cgaccctgcc tgcggctgcg gcaacttcct tatcatcgcc tacgaccgca tccgcgccct
     9361 tgaagacgac atcatcgccg aagccctcaa agacaaagca ggcggcctgt tcgacagccc
     9421 gtccgtccaa tgccgtctga aacagtttca cggcatcgaa atagacgaat ttgccgtcct
     9481 catcgcccgc accgccatgt ggctcaaaaa ccaccaatgc aacatccgca cgcaaatccg
     9541 cttcgacggc gaagtcgcct gccatacgct gccgctcgaa gacgccgccg aaatcatcca
     9601 cgccaacagc ctccgtacac cttggcaggc ggcggactac atcttcggca atccgccctt
     9661 tatcggctcg acctaccaaa ccaaagagca gaaaaacgac ctcgaaagca tctgcggcca
     9721 tatcaaaggc tacggcctgt tggattacgt ctgcaactgg tacgtcaaag ccgccggcat
     9781 catggcacag aatccccaag ttcagacggc atttgtttcc accaattcca tctgccaagg
     9841 ccagcaggtc gaaatccttt ggggcagcct tttgaagcaa ggcatcgaaa tccactttgc
     9901 ccaccgcacc ttccaatgga cgagccaagc cgcaggcaaa gccgccgtcc actgcatcat
     9961 cgtcggcttc cgccaaaagc cgcaaatgcc gtctgaaaaa accctctacg actatcccga
    10021 catcaaaggc gaaccccaaa aacacgccgc ggccaacatc aatccgtatc tgattgacgc
    10081 gcccgatttg attattgcca agcgcagccg tcccatacat tgcgaacctg atatggtcaa
    10141 cggaagcaaa ccgaccgaag gcggcaacct tatcctttca accgccgaaa aagatgccct
    10201 gattgccgcc gagcccttgg cggagcaata catccgcccc tttatcggtg cggatgagtt
    10261 cctcaacggc aaaacccgtt ggtgcctgtg gtttcaaggc atatccgacg ttaaacgcaa
    10321 ccacgacctg aaacagatgc ccaaagttca agcccgtatt caggcagtca aaaccatgcg
    10381 cgaagccagc agcgacaaac aaactcaaaa agatgcagca accccgtggc tttttcaaaa
    10441 aatccgccag ccttcagacg gcaattttct gattatccca cgcgtctcat ccgaaagccg
    10501 ccgtttcatc cccatcggtt atctatcata tgaaacagtt gtcagcgatt tagtatttac
    10561 ccttccaaac gccaccctct accacttcgg catcctcagc tccaccatgc acaacgcctt
    10621 tatgcgtacc gttgccggcc gtctggaaag tcgttaccgc tattccaata ccgttgtgta
    10681 caacaacttc ccattccccg aaagctgccg gatgccgtct gaaaacgacc gccaagaccc
    10741 gctccgcgcc gccgtcgaaa ccgccgccca agccgtcctc gacgcgcgcg gacaataccg
    10801 ccgagaagcg cgggaagccg gtttgcccga gccgaccctc gccgaactct atgcgcccga
    10861 cgcaggctat accgccctag acaaagccca cgccgccctc gacaaggcag tcgataaagc
    10921 ctacggctac aaaacaggca aaaacaccga cgacgaagcc gaacgcaccg ccttcctgtt
    10981 cgagctgtac cgcaaggcgg cggcaattgc gtagtagttg cgtaggatag ttgcgtaggg
    11041 tgggcttcag cccaccgttt ttcttcattt tcggttgttg attggcaggc tgaagcccac
    11101 cctgcggtta caaaaatccc gtcattcccg cgcaggcggg aatccagacc tttcggggcg
    11161 gaaacttatc ggataaaacg atttcccctg catcctggat tcccactttc ataggaatga
    11221 cgggatatag gtttccctac ggacgtgttc agattcccgc cttcgcggga atgacggcgg
    11281 agaagtttct gtctttccga taacttaccg taacttaaaa tcccgtcatt accgcaaaag
    11341 cagttgctac ggttacaaaa atcccgtcat ccccacgaaa gcgaaaatcc tgcctgtcgg
    11401 atttcggttt tttcgggcgt ttcgggaact tataaatcgt cattcccgcg caggcgggaa
    11461 tccagaacgc gggacggcgg caatattcaa aggttgtctg aaaattcaga ggttctagat
    11521 tcccgcctgc gcgggaatga cggcggagcg gtttctgctt ttccgataaa tgaccgcaac
    11581 ctaaaaccca tccttcccac gaaaacagaa aaacaaaaac ctaaaatccc gtcgttctcg
    11641 cgaaagcagt tgctacgttt accgtcaaaa atgccgtctg aacgctcaag ccttcagacg
    11701 gcatttttta tttcaaaccc ttacaggtag aatttttcga ggactttcag gttgtcatcc
    11761 aatttgtaca ccagcggctg accggtcggg atttccaagc ccataatgtc ttcgtcggaa
    11821 atgccctcga tgtgttttgc cagcgcgcgc agggagttgc cgtgcgccgc caccaagacg
    11881 cgtttgccgc tcaaaatcgc gggggcgatt tggtcttccc aaaacggtaa tacgcgctcc
    11941 agcgttactt tcaggttttc gccgtcgggt acgacatcgg caggcagatg ggcatagcgg
    12001 cggtctttgt gtgcggaaaa ctcatcgtct ttgtccaaaa gcggcggcag ggtgtcgtag
    12061 ctgcgccgcc agatgcggac ttgctcgtcg ccgtattgtt cggcggtttg ttttttgtcc
    12121 aggccttgca gttggccgta gtggcgttcg ttcagccgcc acgttttgat ttgcggtacg
    12181 aacagttggt cggattcttc caaaacgatg ttgcaggtct taatcgcgcg ggtcaggacg
    12241 gatgtgaagg cgatgtcgaa ctcatagccg ttttctttca gtttcttgcc ggcggcggcg
    12301 gcctcggcaa gcccctgccc gctcagcttc acgtcgcgcc agcctgtaaa caggtttttc
    12361 gcgttccatt cgctttgtcc gtggcggata aataccagtt ccatatcgtc tccaatgtgt
    12421 gaaagtggga aagccttatt tataacatat tttcacattt cccgtatttg attcagattc
    12481 agacacgcgc ccactatggt ttgccgtttt gatttacaat aatgcccttt gctttacatt
    12541 ccgcatacac aatgtttcaa cacacaggac gacacataaa gcaccgccct atgtgttgcc
    12601 ctgatttgga aggggttgcc ccctcccaaa taaagtctga ttctaccgcc ccgaaggaca
    12661 gatgtccaag tggcggggtt tcaaccgaaa aggaaatacg ataaatatgc aaccgcacgc
    12721 tccacatacc gattccaata cgctgatgct cggccgatac gccgaacgcg cctatctcga
    12781 atacgccatg agcgtggtca aaggccgcgc gctgcctgaa gtttcagacg gccaaaagcc
    12841 cgttcagcgg cgcattttgt ttgccatgcg cgatatgggt ttgacggcgg gggcgaagcc
    12901 ggtgaaatcg gcgcgcgtgg tcggcgagat tttgggtaaa taccacccgc acggcgacag
    12961 ttccgcctat gaggcgatgg tgcggatggc gcaggatttt accttgcgct atcccttaat
    13021 cgacggcatc ggcaacttcg gctcgcgcga cggcgacggg gcggcggcga tgcgttacac
    13081 cgaagcgcgg ctcacgccga ttgcggaatt gctgttgtcc gaaatcaatc aggggacggt
    13141 ggatttcgtg ccgaactacg acggcgcgtt tgacgagccg ctgcacctgc ccgcccgcct
    13201 gcctatggtc ttgctcaacg gcgcgtcagg catcgcggtg ggcatggcga ccgagattcc
    13261 gtcgcacaat ttgaacgaag tgacgcaggc agcgattgcg ctgttgaaga agccgacgct
    13321 ggaaaccgcc gacctgatgc aatacattcc cgcccccgat tttgccggtg gcggtcaaat
    13381 catcacgccg gcggacgaat tgcgccgtat ttacgaaacc ggcaagggca gcgtgcgcgt
    13441 gcgtgcacgt tatgaaattg aaaaattggc gcgcggacaa tggcgcgtca tcgtaaccga
    13501 gctgccgccg aacgccaact ccgccaaaat ccttgccgaa atcgaagaac aaaccaaccc
    13561 gaaaccgaaa gcgggtaaga aacagctcaa ccaagaccag ctcaacacca aaaagctgat
    13621 gctggattta atcgaccgcg tgcgcgacga gtccgacggc gaacatcccg tgcgactggt
    13681 attcgagccg aaatccagcc gcatcgatac cgataccttc atcaacacgc tgatggcgca
    13741 aacttcgctg gaaagcaatg tgtccatgaa cttggtgatg atggggctgg acaaccgccc
    13801 cgcgcagaaa aacctgaaaa cgattttgca ggaatggctg gatttccgca ccgtaaccgt
    13861 aacacgccgt ctgaaattcc gtttgaacca agtggaaaaa cggctgcaca tcctcgaagg
    13921 ccgtctgaaa gtctttctgc acatcgacga agtgattaaa gtcatccgcg aatcagacga
    13981 ccccaaaacc gacctgatgg cggcgttcgg gctgaccgaa atccaggccg aagacatttt
    14041 ggaaatccgc ctgcgtcagt tggcgcgttt ggaaggtttc aaactcgaaa aagaattgaa
    14101 cgaattgcgc gaagaacaag gccgtctgaa catccttttg ggcgacgaaa acgaaaaacg
    14161 caagctgatt atcaaagaga tgcaggcgga catgaaacag ttcggcgacg cgcgacgcac
    14221 gctggtggaa gaagccggac gcgccgtgtt gacgcagacc actgccgacg aaccgatcac
    14281 actgattctg tcggaaaaag gctggatacg cagccgtgcc ggacacaatc tcgatttgag
    14341 ccaaaccgcg ttcaaagaag gcgaccgcct caaacaaact cttgaaggcc gcactgtttt
    14401 gcccgtcgtc attctcgatt catcgggcag aacctacacg ctcgatgctg ccgaaatccc
    14461 cggcgggcgc ggcgacggcg taccggtttc atccttgatc gagttgcaaa acggcgcgaa
    14521 acccgttgcg atgctgacag gattgccgga acaacattat ttattatcgg gcagcggcgg
    14581 ctacggcttc atcgccaaac tgggcgatat ggtcggacgc gtgaaagcgg gcaaagtggt
    14641 gatgaccgca gacagcggcg aaaccgtcct gccgccggtt gccgtctatg cctcctcgtt
    14701 catcaacccc gactgcaaaa tcattgccgc caccaatcaa aaccgcgccc tcgccttccc
    14761 catcggcgaa ttgaaaatta tggcgaaagg caaagggctg caaatcatcg gattaaacgc
    14821 cggcgaatcg atgacgcata ccgccgtttc ttccgagctg gaaatcctga ttgaaagcga
    14881 aggcaggcgc ggcgcagcgc acaaagaccg catccccatc tccctgcttg aggcaaaacg
    14941 cggcaaaaaa ggcaaactat tacccatatc gggcagcctg aaacagcttt cttccccgaa
    15001 ataaacccga ctctgcacat actatggtaa tttccaatac ccgcgaactt gagaaactca
    15061 aagaccggat tcccaatctg atcaacatcg cccgcatcgc catcgtcttc cccctgctga
    15121 ttatgcatat cttcgggttg gaggtcagcg gacatgaaaa cctgcacgct tcgtggacgg
    15181 cgtgggcgtt ttatgtttgg ctcgccattg cctgctggct gattttcttt tccatcatcc
    15241 atccgcattg gcaatggcag gctttgagaa taccgagttt cagcgcggtg gcggacatca
    15301 cgatgatcgg cgtgctgacc tacctgttcg gcggcatcga ctccggcttc ggcatcctga
    15361 ttctgccctt cgtcgtctgc tcctgcctgc tcagctacgg gcgttacccc ctgctctatg
    15421 ccagctacgc cgccatcctg ctgatattga atgcccttgc agacagcaat atcaatatgt
    15481 atccgctgat tctggatgca aaaaccgtca ccaacacctt cgtcctcgtg gctggttcct
    15541 atttcgtcgc catgattgcc tcactgtcgg tcagatacat cgaccgtgcc ggaaaactcg
    15601 cctacgacag ccatatcgcc taccggcgca tcaaaggctt gagccaaacc gtactcaacc
    15661 gcgttcagga agcggtcgtc gtcatcaacg tcgagcatca gaccatactg ttcaataaaa
    15721 aggcaaaaga tctgctcccc atgcttgaaa tcggacagca taccgcccta ttcgacccta
    15781 ttaccgtttt atgggacaag gcctcttcgc gtactttcga acgcaatatc gacacgcccg
    15841 ccctgaccgc ccgcatccgc gccgtgccga tgaacaaaga gcaaaacaaa ctgctcatcc
    15901 tctacatccg cccgcaaaac gaaattcagg cagaagccct gtccgtcaaa cttgccgcac
    15961 tcggacaact gaccgccaac ctcgcccacg aaatccgcaa ccccatgtcc gccattcgcc
    16021 acgccaacga cctgctgcgc gaaaatatgg aagcgggggc ggcagatccg ttcaacgcca
    16081 aattgtgcaa aatcatcgac ggcaacgtct gccgtatcga caaaatgctc gaagatattt
    16141 cctcgccaac aagcgcaaca aaaccgaacg cgaaaccatc ggcctgatac cgttttggga
    16201 agaattcaaa caagagttcc tgctcggcca tcccgatgcc gccggctgca tccgcctaga
    16261 tatgcagggc aatcacctga ctgcctattt cgatcctgcc cacctgcggc aaattatgtg
    16321 gaacctcgcc aacaacgcgt ggcggcacag ccgcaaacag cccggctcga tttccgtcac
    16381 catccgcccc gcgcaaaaaa acaccgtctg catcctcttt gccgaccgcc cgaagtgcag
    16441 gaacacctgt tcgaaccctt ttacaccacg gcggaaaacg gcaccggcct cgggctgtat
    16501 gtcgcccgcg aactggcaca tgccaacttc ggcgatttga cctacctgcc cgaagccaaa
    16561 tgtttcgaac tcgcattacc ggaaaaaacc aatgactgaa ctgcaacacc ccgtcctcgt
    16621 cgtcgataac gaaaccgaca ttctcgacct gatggaaatg accctgatga aaatgggctt
    16681 gcgcgtccat accgcgtcag gcgttgccga agccaaaaac aagctcgaca gccaacgcta
    16741 ttcgctcgtc ctgaccgata tgcgtatgcc ggacggctcg gggctggaag tcgtccaaca
    16801 catcaacagc cgcctgctcg atacgccggt tgccgtcatc accgccttcg gcaacgccga
    16861 tcaggcacag gaagcgttgc gttgcggcgc gttcgacccc gataccatgc agatacagga
    16921 ctatctcgac caaatcgaac gcgacattat cgaacaaacc ctcaaacaaa ccgaaggcaa
    16981 ccgcacgcag gccgccaaac gcttgggcat cagcttccgt tccatgcgct accgtatgga
    17041 acgcctcaac atcggctgac gacaaaacgg catccgcacc atctccgccc acccgaaaaa
    17101 atgccgtctg aaacggcacg gcaaagcggg ttcgccccac gcccgaacgg acacaaaaca
    17161 ccatgaccga catcctcatt gacaacaccg ccaccgaaac cgtccgcacc ctgatacagg
    17221 cattccccct tgtgcccgtt tcccaaccgc ccgaacaagg cagttacctc cttgccgaac
    17281 acgataccgt cagcctcagg cttgtcgggg aaaaaagcag cgtcatcgtc gattttgcct
    17341 ccggcgcggc acaataccgg cgcacaaaag gcgggggcga actcatcgcc aaagccgtca
    17401 accacaccgc gcaccccacc gtttgggacg caaccgcagg attggggcgc gacagcttcg
    17461 tcctcgcctc gctcgggctg gccgttaccg ccttcgagca acatcccgcc gtcgcctgcc
    17521 tgctttcaga cggcatccgc cgcgccctcc tcaatcccga aacgcaagac accgccgcgc
    17581 gcatcaacct ccatttcggc aacgccgcag tacaaatgcc cgcccttgtt caaacacaag
    17641 gcaaacccga catcgtttat ctcgacccga tgtatcccga acgccgcaaa agtgccgccg
    17701 ttaaaaaaga aatggcctac ttccaccggc tcgtcggcga agcgcaagat gaagcggcac
    17761 tcctccatac cgccagacag actgcaaaaa aacgcgtcgt cgtcaaacgc ccccgcctcg
    17821 gcgaacacct tgccggacaa gcccctgcct accaatacac cggcaaaagc acccgcttcg
    17881 acgtttacct gccctacggg acggacaagg gataacgccc ataaaacaag acaccgaaaa
    17941 atttgccgtt cttatgcaaa cgagaaaccg gtttttgcgt ttcgactgtt ttggataagt
    18001 catcacacct taaagtttgt cattcccaca gaagtgggaa tccgattcat tcagttttat
    18061 agtggtttaa atttaaacca ctatagctgt tttcgagttt caggcaactt ccaaaccgtc
    18121 attcccacgg aagtgggaat ctagaaatga aaggcaacag gaatttatcg taaatgaccg
    18181 aaaccgaacg gactagattc ccgcctgcgc gggaatgacg gggcggg
//
LOCUS       DAWFJR010000036        18107 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWFJR010000036 DAWFJR010000000
VERSION     DAWFJR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18107
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.036"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            16..1170
                     /gene="lpxB"
                     /locus_tag="WGR04_001108"
     CDS             16..1170
                     /gene="lpxB"
                     /locus_tag="WGR04_001108"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ0794673.1"
                     /translation="MVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARFVGIGGE
                     LMKAEGFESLYDQERLAVRGFAEVVKRLPEILRIRRGLVRDLLSLKPDVFVGIDAPDF
                     NLGVAEKLKRAGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLYLDAGGR
                     AEFVGHPMAQLMPLEDDRKTARKTLGVDAGIPVFALLPGSRVSEIDYMAPVFFQTALL
                     LLERYPAACFLLPAATEATKRRLAEVLQRPEFAGLPLTVTDRQSETVCRAADAVLVTS
                     GTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPELLQSEAK
                     PEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            1178..1423
                     /locus_tag="WGR04_001109"
     CDS             1178..1423
                     /locus_tag="WGR04_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794674.1"
                     /translation="MDYLLEAARITKEGGEIVINGTSNNKYLRGIPNEAELARMGLRL
                     KYNGLLKEEFKQLKFHLSNGGDLRSSNYKTIVFEKVK"
     gene            1441..1836
                     /locus_tag="WGR04_001110"
     CDS             1441..1836
                     /locus_tag="WGR04_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794675.1"
                     /translation="MPKKDYQFLYIYEGTLLNYTLKNDEFHIIVKDVDYPDFPQEIPM
                     PNYSEWVKIKFKQFSYLKFIYGYATKNQDKNIKNVLELGELKQDDEILDYGGALEVIG
                     GRYDLPTGFSIDIVCREIELEFLDQESFN"
     gene            complement(2100..2909)
                     /gene="dapB"
                     /locus_tag="WGR04_001111"
     CDS             complement(2100..2909)
                     /gene="dapB"
                     /locus_tag="WGR04_001111"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ0794676.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKASSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2919..3296)
                     /locus_tag="WGR04_001112"
     CDS             complement(2919..3296)
                     /locus_tag="WGR04_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0794677.1"
                     /translation="MNKTLILALSALLGLAACSAERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDVLQNAAEALKDRQNTDKP"
     gene            3515..3949
                     /gene="fur"
                     /locus_tag="WGR04_001113"
     CDS             3515..3949
                     /gene="fur"
                     /locus_tag="WGR04_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ0794678.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            4019..4744
                     /locus_tag="WGR04_001114"
     CDS             4019..4744
                     /locus_tag="WGR04_001114"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ0794679.1"
                     /translation="MRIPLLAPGNYAFPDPAYALARCDGLVGVSGDLDTGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRHEVACKGL"
     gene            complement(4888..5919)
                     /gene="gap"
                     /locus_tag="WGR04_001115"
     CDS             complement(4888..5919)
                     /gene="gap"
                     /locus_tag="WGR04_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0794680.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKIHLKSGAKKVLISAPGGDDVDATVVYGVNDSILTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(6119..6958)
                     /locus_tag="WGR04_001116"
     CDS             complement(6119..6958)
                     /locus_tag="WGR04_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ0794681.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRAHRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            7107..9083
                     /locus_tag="WGR04_001117"
     CDS             7107..9083
                     /locus_tag="WGR04_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ0794682.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVIAGKKEEIISFENWSLQGI"
     gene            9153..9228
                     /locus_tag="WGR04_001118"
     tRNA            9153..9228
                     /locus_tag="WGR04_001118"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9186..9188,aa:Val,seq:tac)
     gene            9261..9337
                     /locus_tag="WGR04_001119"
     tRNA            9261..9337
                     /locus_tag="WGR04_001119"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9295..9297,aa:Asp,seq:gtc)
     gene            9382..9457
                     /locus_tag="WGR04_001120"
     tRNA            9382..9457
                     /locus_tag="WGR04_001120"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9415..9417,aa:Val,seq:tac)
     gene            9491..9567
                     /locus_tag="WGR04_001121"
     tRNA            9491..9567
                     /locus_tag="WGR04_001121"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9525..9527,aa:Asp,seq:gtc)
     gene            10139..10237
                     /locus_tag="WGR04_001122"
     CDS             10139..10237
                     /locus_tag="WGR04_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ0794683.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(10308..11684)
                     /locus_tag="WGR04_001123"
     CDS             complement(10308..11684)
                     /locus_tag="WGR04_001123"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEZ0794684.1"
                     /translation="MHYIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDIYGS
                     LALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLERIRTQHILRLNGQEIRFIPDRD
                     LNILGNQVLPKHPNSLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEKAVP
                     YPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIKRGL
                     GADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRVVTA
                     PTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGCQGE
                     VGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNGIAA
                     EKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKKAVP
                     VSVRVVEC"
     gene            complement(11946..14336)
                     /gene="gyrB"
                     /locus_tag="WGR04_001124"
     CDS             complement(11946..14336)
                     /gene="gyrB"
                     /locus_tag="WGR04_001124"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ0794685.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLNV
                     VGDSDKKGTTVRFLASAETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIETNEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIISDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENT
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            14606..15478
                     /locus_tag="WGR04_001125"
     CDS             14606..15478
                     /locus_tag="WGR04_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0794686.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRGTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            15566..17602
                     /locus_tag="WGR04_001126"
     CDS             15566..17602
                     /locus_tag="WGR04_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ0794687.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAAYNELMPEITAFFTEIGQ
                     DIALYNRFKTIKNSPEFATLSTAQKTKLNHDLRDFVLSGAELPPEQQAELAQLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELANDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YDVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKEVAVVRPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESGDIAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLSHSGFDNAA"
BASE COUNT         3763 a         4505 c         5422 g         4417 t
ORIGIN      
        1 tggggacaga cgaatatggt tgataaaaaa agccctttga ttgccgtcag tgtcggcgaa
       61 gcgtcgggcg acctgttggg ggcgcacctg atacgcgcca tccgcaagcg ttgtccgcag
      121 gcgcgttttg tcggtatcgg cggcgaactg atgaaggcgg aaggtttcga gagcctttat
      181 gatcaggagc ggctggcggt gcgcggtttt gccgaggtgg taaaacgcct gccggaaatt
      241 ttacggatac gcagggggct ggtacgggat ttgctgtcgt tgaaacctga tgtgtttgtc
      301 ggtatcgatg cgcccgattt taatctgggt gtggctgaaa agctgaaacg ggcgggcatt
      361 ccgaccgtgc attatgtcag cccgtcggtg tgggcgtggc ggcgggaacg cgtgggcaaa
      421 atcgtgcatc aggtcaaccg cgtgctgtgc ctgttcccga tggagccgca gctttatctc
      481 gatgcgggcg gacgtgcgga gtttgtcggt catccgatgg cgcagcttat gcccttggaa
      541 gacgaccgta aaacggcgcg gaaaactttg ggcgtggatg ccggcatccc cgtattcgcc
      601 ctgctgcccg gcagccgcgt cagcgaaatc gattatatgg cgccggtgtt ttttcagacg
      661 gcattattgt tgttggaacg ctatcctgcc gcatgcttcc tgctgcctgc cgcaacggag
      721 gcgacgaagc ggcgtttggc ggaagttttg cagcggccgg agtttgccgg cctgccgctg
      781 acggtaaccg acagacagtc tgaaacggtg tgcagggcgg cggatgcggt gctggtaacg
      841 agcggtacgg caactttgga ggtggcgctg tgcaagcgtc cgatggtcat cagctacaag
      901 atttcgccgc tgacctatgc ttatgtgaaa cgcaaaatca aagtgccgca tgtcggcctg
      961 ccgaatatcc tgttgggtaa ggaggctgtg ccggaattat tgcaatctga agcaaaaccg
     1021 gaaaaactgg cggcggcgtt ggcggactgg tacgaacacc ccgataaggt tgccgcgctg
     1081 caacaggatt tcagggcgtt gcacctgctg ttgaaaaaag atacggcgga tttggccgcg
     1141 cgcgcggttt tggaagaggc gggatgttga gcggttaatg gattatcttc ttgaagcagc
     1201 acgtattaca aaagaagggg gagaaattgt gattaatggc acatcaaaca ataagtattt
     1261 aagaggaatt ccaaatgaag cagaactggc ccgaatggga ttaaggttaa aatataatgg
     1321 tctgttaaag gaagagttta aacaactgaa atttcatctg agcaatggag gcgacttgag
     1381 atcaagtaat tataaaacca ttgtttttga aaaggtaaaa taatatgaca acttaatact
     1441 attccaaaaa aagattatca atttctgtat atatatgagg gcactctatt aaactatact
     1501 ttgaaaaatg atgagttcca tatcatcgtt aaggatgtgg attatccaga ctttcctcaa
     1561 gagatcccta tgcccaatta ttcagaatgg gtaaaaatta aattcaagca gttcagctat
     1621 ctgaaattta tctatggata cgccacgaag aaccaagata aaaatatcaa aaatgtattg
     1681 gaacttggag aattaaagca ggatgatgaa atcttggatt atggaggtgc gctggaagtg
     1741 ataggcggta ggtatgatct tccgaccggt tttagtatag atatagtttg ccgggaaata
     1801 gagttagaat ttttagatca ggagagtttt aattaaacga gccgtaactt gttatgctga
     1861 gcaggcaact ttatcgtatt tccttttcgg ttgaaacccc gccactcgga catctgtcct
     1921 tcggggcggt agaatcagat tttatttggg aggggcgtaa ccccttccga atcagggcaa
     1981 cacatagggc gacgctttat gtgtcgtcct gtgtgttgaa acattgatat gccgatacgg
     2041 agcctgtcgg caaaatgccg tctgaacaat atcttttcag acggcatttt gtatgggggt
     2101 taacggttgt tcagcccgag tacgtcctgc atatcgtaca aacccgtttt gccgttgacc
     2161 caaactgcgg cgcggacggc accggcggca aaggtcatgc ggctgctggc cttgtgggtg
     2221 atttccacgc gctcgccgtc ggtggcgaag agggcggtgt ggtcgccgac gatgtcgcct
     2281 gcgcggacgg tggcaaagcc gatggtcgac ggatcgcgcg gaccggtgtg gccttcgcgg
     2341 ccgtaaacgg cgcattgttt gaggtctctg ccgagcgcgc cggcgatgac ttcgcccatg
     2401 cgtaacgcgg tgccgctggg ggcatcgact ttgtggcggt ggtggccttc aatgatttcg
     2461 atgtcgtagc cttcgtttag tacgcgtgcg acggtgtcga ggatgtggaa ggtgaggttg
     2521 acgccgacgc tgaagttggc ggcgaaaacg atgcctgttt gttcggcggc agtgtggata
     2581 gcggctttgc ccgcatcgtc gaagcctgtt gtgccgatga tgatgttgac ttgtttttca
     2641 acgcattttt gcaggtgttt gagggtgggc tcggggcggg tgaagtcgat gagtacgtcg
     2701 ctttgtgcga gaacggcgtc aacgtcgtct gaaatggcga tgccggtttt gagtccgacg
     2761 gcgtagcctg cgtccagccc gagggcttct gagcccgagt gttcgagcgc gccggaaagg
     2821 acggtgtcgg gatggttgtt gacggcttca accaatacgc gtcccatacg gccgtttgcg
     2881 ccggcgatgg cgattttgag cggtgtcatg tgtgttcctt atggtttgtc tgtgttttgg
     2941 cggtctttga gggcttcggc agcgttttgc aggacgtcgc cttcggtgcg gacgagtacg
     3001 ccgttttcaa aatagacggt cagattgctg cgttctttga tgatgccgtt gcgggaggtg
     3061 ttgaaggtat agtcccagcg gtcggtatgg aatgcgtcgc gcagtatggg gctgccgagc
     3121 aggagcagga cttggtcttt ggtcatgccg gggcggaggg cggcaacggc gcgcggttcg
     3181 agttcgttgc cctgtatgat tttgagtttg tacgagggga acagtgaaac gcgttcggca
     3241 ctgcacgcgg caaggccgag gagggcggaa agggcgagga tgagggtttt gttcacggaa
     3301 atgcctttct gtgcaaatcg ggatgggtag tgtaacactg cttgaatatt ttataaaagc
     3361 gaacgataat catacgatta agcggtatcc gccctgtccg cgcatcggcc gccggtgcgg
     3421 ttttactatt gcaaactgct atggtgcgat agtgggcaaa caggccgaaa ttgcgtatta
     3481 taacgtctat tgttttacag gggtattgaa tattatggaa aaattcaaca atattgcaca
     3541 actgaaagac agcggtctga aggttaccgg cccgcgtttg aagattttgg atttgttcga
     3601 gacgcatgcg gaagagcatt tgagtgcgga agatgtgtac cgcattttgt tggaagaggg
     3661 tgtggaaatc ggtgtggcga cgatttaccg tgtgctgacc cagtttgagc aggcgggtat
     3721 tctgcaacgc catcattttg aaacgggcaa ggcggtttat gagttggaca aaggcgacca
     3781 ccatgaccac atcgtctgcg tgaagtgcgg cgaggtaacg gaattccaca atcccgaaat
     3841 cgaagccctg caagacaaaa tcgcggaaga aaacggctac cgcatcgtcg atcacgcgct
     3901 ttatatgtac ggcgtgtgca gcgactgtca ggccaagggc aaacgttaaa tccggacggt
     3961 ttgttgttca gacggcattc atgattttgg atgccgtctg tgtttttgga gaactgccat
     4021 gcgtattccg ctgcttgccc ctggcaatta tgcctttccc gatcctgcct atgctttggc
     4081 ccggtgcgac gggctggtcg gcgtgagcgg cgatttggat acggggcggc tgcttgaggc
     4141 gtatcggaac ggcgtgtttc cgtggttttc tcgggacggt tggttttttt ggtatgcggt
     4201 cggcccgcgt gcggtgattg ttcccgaacg tctgcacgtt ccgcgctcgc tggcgaaaac
     4261 gctgcgcaac ggcagctatc gggttgcggt caacggctgt tttgcggaag tggtcgcgca
     4321 ttgtgcggca gcggcgcgcc cgaatcagga cggaacttgg attgcgcccg agtttcagac
     4381 ggcatatttg aagctgcacg aaatgggaca cgcgcattct ttcgagtgcc attatcccga
     4441 tgaaagcggt gaaacgaggt tggcgggcgg cttttacggc gttcagatcg gcagggtgtt
     4501 ttacggcgaa tcgatgttcg cattacaacc ggatgcgtcg aaaatcgcgt ttgcctgcgc
     4561 cgtgccgttt ttggcggatt tgggcgtgga actgatagac tgccagcagg acacggaaca
     4621 tatgcgccgt ttcggttcgg agctgctgcc gtttgcggat tttgccgaac gtctgcggat
     4681 gttgaacgcc gtgccgttga aagaggaaat cgggcggcac gaagtggcgt gcaaggggct
     4741 ttgatggcgg cttatgcttc ggtcaggttc aaatatggcg gattaaaatt ggaaatgacg
     4801 acagccgggt aaaatcacgg tgaaaaatgc cgtctggaac ttgaaaacat cgggtttcag
     4861 acggcatttt gtttgacggt ttattgctta tttgagcggg cgcacttcaa gtccgaacat
     4921 gcggcgtgca gtgttcagca tttgacagct gaatccccat tcgttgtcat accatgcgaa
     4981 gactttgacc atgttgccgt caacgacttt ggtcagtgtg gcatcgaagt ggctggcttc
     5041 ggtggtgtgg ttgaagtcca tggaaaccaa gggcagggtg ttgtagccta aaacgccttt
     5101 gagcgcgcct gcttccgagg cggctttcat cagtgcgttg atttcttcga ctgtggtgtc
     5161 gcgcgcggct tggaagctca aatctaccaa tgatacgttg acggtcggca cgcggatggc
     5221 aagcccgtcg agcctgcctt tcaattcggg cagtaccaaa ccgacggctt ttgccgcgcc
     5281 ggttttggtc ggaatcatgt tttccacgcc gctgcgggcg cggcgcaggt ctttgtggcg
     5341 cacgtcggta acggtttggt cgttggtcag cgcgtggatg gtggtcatcg cgcctttgac
     5401 gatgccgacg ctttcgctca acactttggc aaccggcgag aggcagttgg tggtgcagga
     5461 agcgttggaa acgacggtca tgtcggcggt caggatgctg tcgttcacgc cgtatacgac
     5521 ggttgcatcg acatcgtcgc cgcccggtgc ggaaatgagg acttttttcg cgccgctttt
     5581 gaggtggatt ttggcttttt ctttgctggt gaacgcgccg gtgcattcca tgaccaaatc
     5641 gacaccgagt tctttccacg gcagttcggc agggttgcgg gtcgagaaga aggggatttt
     5701 gtcgccgttg acgatgaggt tgccgccgtc gtgggatacg tcggcttcaa agcgtccgtg
     5761 cacggtgtcg aatttggtca gatgggcgtt ggtttcaagg ctgccgctgg cgttgacggc
     5821 gacgatttgg agttggtctt gaatctgata gtcgtagatg gcgcgcaaaa cctggcggcc
     5881 gatgcgtccg tagccgttga tggcgacttt gatgcccatg gttcgttcct ttgttgaggg
     5941 ttgggtagat tttcggggcg gattatagca aatttgtagt ggcgtgtaat taatatttta
     6001 ttgaaaacgg cgcggccgga agggtgggcg gtaagatgcg gacggcacgg gtgcggcgga
     6061 cggagagctt gataaaatgc cgtctgaaac ggttttcaga cggcatatca gggaagggtc
     6121 aggaggcggt attctgtgcg gcttcctgtt tggctttgta ttgtttgagg tattcgaggg
     6181 cggcggcttt ttcactgtcg ctgccgtatt tcatatcgcg ttgggcgcgg cgcaactcgg
     6241 cgcgttcgcg ggcttcggct atctgtttcg cctgatagtc tttgcggttg tcggcggcgg
     6301 cgaggcggtc ttgttgggtt tgcgcttttg ccatggcttt ggcgatgagg tcggcagggt
     6361 taaacgccgg ttttttcggt gtgtcgggcg tttgcggacg cgcgttacgg acggcggctt
     6421 cgcgttcggc aagcatggcc ttgcgttcgt cggcttcgcg ctgtttgcgt tcgttgcgtt
     6481 tgaggtagcg cgcgcgcgcg tgttcggcgg cggcaaaacg gctgtcggcg gacaggctga
     6541 agcggtgcgc gcggggcagg acggtgtcgg caacgggctg catatggatg cagtcgacgg
     6601 ggcagggggc gacgcagagt ccgcagccgg tgcattcgtc ggcgatgacg gtgtgcataa
     6661 gtttgcccgc gcccataatg gcatcggcag ggcaggcgcg gatgcaggcg gtgcagccga
     6721 tacaggcggt ttcgtctatc cgggcgagtg ctttggcttg ggttttggca ggtgcgacaa
     6781 ggggtttgcc gagcagggcg gaaatgtccc gaatgacggc ttctccgccc ggggcgcaga
     6841 ggttgtacgc ttcgcctgtt gcgactgcct gtgcgtaggg caggcagccg tcgtagccgc
     6901 attcgcggca ttgggtttgg ggaagcaggc ggtctatggc ggcggctgtg gcggtcatgt
     6961 cggtgtgcgg ctcaaaatcg aaagggcgta ttttagcaga attgtatgcc gcgcccgttt
     7021 cggatggtgc gcggtgtttt gttataatac ggcggcgtat gccgtttcag acggcatttt
     7081 tctgtatttt cctgttcgga cggcctatga acgaattttc gcttgcccct attgtggttg
     7141 ttttgctggt gtcggtcatt acggtgatcc tgtgccgcaa gttcaacatt ccctccatgc
     7201 tgggctacct gctggtgggc tttttggcgg ggcccggtat gctcagcctg attccgaaaa
     7261 gccatgcgac ggattatttg ggcgaaatcg ggattgtgtt cctgatgttc agcatcggtt
     7321 tggagttctc gctgcccaag ttgagggcga tgaggcggct ggtgttcggg ctgggcggtt
     7381 tgcaggtcgg cgttacgatg ctgtcggtaa tgggcatact gatgctgacg ggcgtgccgt
     7441 tcaattgggc gtttgccgtg tcgggcgcgt tggcgatgtc gtccacggcg attgtgagcc
     7501 ggattttgtc ggaaaagacg gaattggggc agccgcacgg tcagatggcg atgggcgtgc
     7561 tgctgatgca ggacatcgcc gtcgtgccgc tgatgattct gattcccgcg ctggcgggcg
     7621 gaggggacgg aaatatttgg gcggccttgg gtttggcgtt tgcaaaaatg ctgctgacgc
     7681 tggggctgct gtttttcgtc ggcagcaaaa ttatgtcgcg atggttcagg atggtggcaa
     7741 aacgcaaatc gtccgaactc tttatgatca atgtgctgct ggtaaccttg ggtgtggctt
     7801 atctgactga gctggaaggt ttgtctatgg cgttgggcgc gtttgtggcg gggatgctgc
     7861 tttcggaaac ggaataccgt ttccaagtcg aagacgacat ccgcccgttc cgcgatattt
     7921 tgctcggctt tttctttatc acggtcggca tgaagctgga cattcaggca ttgatcggcg
     7981 gctggcggca ggtattgatg ctgttggcaa tgctgctggt gttgaaggca ctggttgtgt
     8041 ttgccattgc cttcaaaatg aaacattcgg tcggcgacag cctcaaaacg gctttgtatc
     8101 tcgcgcaggg cggcgagttc ggcttcgtga tgctggccat tgccgggcag cttgatatgg
     8161 tttcgccaga atgggaacag gcggcgacgg cggcggttct gctgtcgatg attatcgcgc
     8221 ccttcctctt gggcggcagc gatgcgctgg tcgggcgttt ggtcaagtca agctgggaca
     8281 tgaagtcgct cgatctgcac agtatgctgg tagaaaccat gagcaagtcc gaccatgtgc
     8341 tgattgtcgg cttcggcagg ggcgggcaga cggtcggacg cgtccttgcc caagaggata
     8401 ttccgtattt cgcgctcgac ttggacattg cgcgggtgca ggttgccaga agtgcgggcg
     8461 aaccggtgtc gttcggcgat gcgaaacgca gggaagtatt ggaagccgcc ggtctgggac
     8521 gggcgaaaat ggtggtggtt acgctcaaca atatgcacga aacgcaacac gttttagaca
     8581 atgtgctgtc catgtatccc aatatgcccg tatatgtgcg cgccaccaac gacgattatg
     8641 tgaaaacgtt taccgatata ggtgcggaag aagccgtgtc ggacaccaaa gaaaccggac
     8701 tcgtgctggc aggctatgca atgttaggca acggcgcgtc gtatcggcac gtctatcaga
     8761 cgatggcaaa tatccgccac agccgttatg ccgcgttgga gggactgttt gtcggtagtg
     8821 atgatgaggc aggattcggc gaaaacggcg aaaccgtccg tcacgccttt cctttggctg
     8881 cagaagcata cgccgtcggc aaaacagtcg gcacgcttcc gatggcggct tacggcatca
     8941 aactcttgtt cgtccgccgc cgcaccggcc ggattgaaaa cccggatgcc tcgtttacat
     9001 tggaaggcgg tgacgtgttg gtgatcgcag gcaaaaaaga agaaattatc tcttttgaaa
     9061 actggagttt gcagggaata taaatgaaat gccgaaataa ggcttgcgcc atttccggtt
     9121 atttggttta ataacgcttt cgcaaatcgc aagggtgatt agctcagttg gtagagcgtc
     9181 tgccttacaa gcagaatgtc ggcggttcga ctccgtcatc acccaccaag tttcctttca
     9241 ttgttgcaaa caatggatgc gcggtggtag ctcagttggt tagagtaccg gcctgtcacg
     9301 ccgggggtcg cgggttcgag ccccgtccgc cgcgccaagt cttaaaaata ctgtgttaac
     9361 tttacagtat taaacataat tgggtgatta gctcagttgg tagagcgtct gccttacaag
     9421 cagaatgtcg gcggttcgac tccgtcatca cccaccaagt ttcctttcat tgttgttaaa
     9481 cagtggatgc gcggtggtag ctcagttggt tagagtaccg gcctgtcacg ccgggggtcg
     9541 cgggttcgag ccccgtccgc cgcgccaaaa gttaaggaat accaacctcc ggttggtatt
     9601 tttttgtttg tatgctttga aaaatgtttg tttccggatt ttgccattcc catccggttt
     9661 tgcgctgtac gatgtgtttt agcgcggact tgctcaaaat cgcatgtgat tccggtattt
     9721 gaggctttga ttagggatgc ggactttcaa tatattttct cagctacaac aacgaaggct
     9781 tgatgtctgt cgggcaggta agggagattt ttgagcgttt cggcaaatat aatttggttc
     9841 aaacggaata tcggcgtttt aaggtagata agacagaaaa ccgtaatcat aaggcaaatt
     9901 cgatattcga atatctgcat attttagaaa agacctttta tagtggatta acaaaaacca
     9961 gtacagcgtt gcctcgcctt agctcaaaga gaacgattct ctaaggtgct gaagcaccaa
    10021 gtgaatcggt tccgtactat ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg
    10081 ttaatccact ataaaaattc ttgccggatg ctgcaaacaa cgccggtttg cattcctgat
    10141 ggcggtggtt ttcttagacg aacgcccgaa cacgcaggaa tggataggct tggggctggt
    10201 tacggcgggc gtgttgacgc tggcactgaa acggtaaagc cgcaagaaat aaatgaaatg
    10261 ccgtctaaaa aactgttttc agacggcatt ttcgtttctg tccatcctca gcactcgacc
    10321 acgcgcacgg atacggggac ggcttttttc cggagcgtgg cggcgagtcc ggcaagcgag
    10381 gtttctttgt aggtcgcctt catatcccgg ccggtttgca gcatggttcg gatgacttcg
    10441 tcgagcgaga cttttttgtc cgtgccgtct tccaaaagcg cgagcgtgcc gagtttgagg
    10501 gctttttcgg cggcgatgcc gttgcgctcg atgcagggga tttgcaccag tccgccgacg
    10561 gggtcgcaag tcagccccaa atggtgttcc atcgccattt cggcggcgtt ttccacttgt
    10621 ttgggcgtgc cgccgatgac ttcggcgtat gcgcccgccg ccatcgaaca cgctacgccg
    10681 acttcgccct gacagccgac atccgcaccg gaaatggagg cgttggtctt gtagaggatg
    10741 ccgattgcgc ctgcggtgag caggaagttt tcgacgcgtt cctgtgtggc gtgcggattg
    10801 aacttgcgga aatagtgcaa tacggcggga atgatgcctg ccgcgccgtt ggtcggtgcg
    10861 gtaacgacgc gtccgccggc ggcgttttct tcgttgaccg ccatggcgta caccatcggc
    10921 cagagctggg tgttgacgat ttcggtttcg cgcaggactt tgagcttggc ggcaagctgc
    10981 ggggcgcggc ggcggacgtt caatccgccg ggcagttcgc cgtccgcacc caagccgcgt
    11041 ttgatgcagc cttccataac ctcggcaacg gcagcggcgc ggcggcggat ttcggcttcg
    11101 ccgcatccgg caagcgcggc ttcgtttgcc aacacgactt cggagatgtc gagccggttc
    11161 agacggcatc gggcaagcag ttcggcgcaa ctggtatagg gatagggaac ggctttttcc
    11221 gtttccgcct gccggtcaaa atcttcttcg gtaacgacaa agccgccgcc gaccgaataa
    11281 taaacctgtt cattcaatac cgtgccgtct gaagcatagg cggtaaaacg caggctgttg
    11341 gggtgtttgg gcagcacttg attgccgagt atgttcaggt cgcggtcggg gatgaagcgg
    11401 atttcttgcc cgttgagccg gaggatgtgc tgcgtgcgga tgcgttcgag gcgttcggga
    11461 atgccggcaa gcgggatgtc gtgcggcagg ctgccttcca aaccgagcat cagcgcgtca
    11521 aatgtaccgt gtccgtatcc ggtcagtgcg agcgagccgt aaatgtcgat gacgatgcga
    11581 acagcctgtg catccaaacc tgccgcaaag gcggcggctg ccttcatcgg gccgaccgta
    11641 tgcgaactgg aaggcccgat accgattttg aaaatatagt ggattaaatt taaaccagta
    11701 cagcgttgcc tcgccttagc tcaaagagaa cgattctcta aggtgctgaa gcaccaagtg
    11761 aatcggttcc gtactatctg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta
    11821 atccactata tcaaaaatgc tgatcatggt tttgctccga aaggcggttt cagacggcat
    11881 ggcttccgtt tgacaaacca aaaggagacg cggcacgatg cccgtctcct tttttaaaac
    11941 ggcacttatg cgtcgatatt ttgggcaatc agcgcgttgt tttcgataaa ggcgcggcgc
    12001 ggttcgacct cgtcgcccat cagggtaacg aatacttcgt cggcggcaat ggcatcttcg
    12061 atgcgcactt tcaacaggcg gcgcacggcg ggatccatcg tggtttccca cagctgttcg
    12121 gggttcatct cgcccaagcc tttgtatcgt tggatggaca tacctttttg ggcaacgctc
    12181 attaagatgt ccaaagcggt ttcaaagctg tccgcgtcgt acccgttttc gcctttgtaa
    12241 agcttggcac cctcgccgac catgcctttg agcgcggcgg cggtttgggt gagggtttgg
    12301 taggctttgc tgttgaggaa cttgggttcg atgtagctga ccatgacgtt gccgtgcagc
    12361 ttgcgcgtga ttttgatgaa ccggtgtcct tcatgacctt cgatgcgttc gagggcgact
    12421 tctttttcgt caagcagacc ggaaagttcg gcaacggctt tatcggtgtt ttcagacgac
    12481 gtcaaatcaa tgggcgacgc gtgtagcatg gcgcgcagga cgagttcgtc tacgaagcgg
    12541 ctttcctgtt cgatgacggt ttttgccaac aggaattgtt tggcggtgtc ggcaagttct
    12601 gcgccttcga tggtgcggcc gtctgaaatg attttggctt tttccaaggc aagaccgagc
    12661 agccattggt ctttttccaa ctcgtccttg aggtaacgtt cctgtttgcc gtatttcgct
    12721 ttatacaaag gcggctgggc gatatagatg tagccgcgct cgaccagttc gggcatttgg
    12781 cggtagaaga aggtcaaaag caaggtgcgg atatgcgcac cgtccacatc ggcatcggtc
    12841 atgataatga tgcggtggta acgcagcttc tcggcattga attcttcttt gccgatgccc
    12901 gcgcccaacg cggtaatcaa agcggctact tcctgactgg cgagcatttt ttcaaagcgc
    12961 gctttttcga cgttcaaaat cttgccctta agcggcaaaa tcgcttggaa tttgcggtcg
    13021 cgcccctgca tggcggaacc gcctgcagaa tcgccctcga ccagataaag ttctgacaat
    13081 gcggggtctt tttcttggca gtcggcgagt ttgccgggta gccccaagcc gtccattacg
    13141 cctttgcggc gggtgatttc gcgggctttg cgggcggctt cgcgcgcgcg ggcggcatcg
    13201 acgattttgc cggtaatgat tttggcttcg gcagggtttt cttcgaggaa gtcggtcagg
    13261 gcttggctga tgacttcgtt gacgacggga ccgatttcgc cggaaaccag tttgtctttg
    13321 gtttgggacg aaaatttggg gtcgggcagt ttgacggaca acacgcaggt caaaccttcg
    13381 cgcatatcgt cgccggcggt ttccactttg gcttttttgg cgacttcgtt ggtttcgatg
    13441 tagctgttga tggtgcgcgt catcacttgg cgcaacgcgg tcaggtgcgt accgccgtcg
    13501 cgctgaggga tgttgttggt gaagcactgc acgctttcct gatagctgtc gttccattgc
    13561 attgcgcatt cgacgctcat gccgtctttc tcgccgaacg cgtagaagat tttttcgtgc
    13621 aagggcgttt ttttgcggtt catgtattgc acgaatccag ccacgccgcc ggaaagggcg
    13681 aagctttcgt gtttgccgtc gcgctcgtcg gtcaattcga tgtccacgcc gttgttcagg
    13741 aacgaaagtt cgcggatgcg tttggcgagg atgtcgaagc tgtattcgac gttgccgaag
    13801 gtttccgcac tggcgaggaa gcgcacggtc gtgccttttt tatcggaatc gccgacaacg
    13861 ttcagcggct cttcggtttc gccgcgcacg aagcggacga agtgttcttt gccgtcgcgg
    13921 tagatggtca gcgttaccca gtcggacagc gcgttgacga cggacacgcc cacgccgtgc
    13981 aggccgccgg agattttgta gctgttgttg tcgaatttac cgcccgcgtg caatacggtc
    14041 atgatgactt cggcggcgga gcgtccttct ttcgggtgga tgccggtggg cataccgcgc
    14101 ccgttgtcgg cgacgctgac ggaatggtcg gcgtgtatcg ttaccgtgat tttgtcgcaa
    14161 tgtccggcga gtgcttcgtc aatggcgttg tccaatactt caaacaccat atggtgcaga
    14221 ccgctgccgt cctgcgtgtc gccgatgtac atgccggggc gtttgcgtac cgcttccaag
    14281 ccttcgagca cctgaatgct gtcggcgccg tattcttcgt gtttttgttc agtcatattt
    14341 tttgccggat tttgaaaaga ttttgcgatg ccgccaaaac aagtccgcac cttgtggaaa
    14401 aagcgggcgg gacggcatat ataattgtgt attatagccg attttgccgc ctaattcagc
    14461 gttatccgca tcagtgtgcc gccgggaaaa gatgaaacgg tacgtttgcc tccggcatca
    14521 ggtcggggat tttcccgtaa agtggcaaaa gcgttttttt gccactaaaa tctacaccct
    14581 atacttttcg gacaggggcg cggaaatgga aatatggaat atgttggaca cttggctcgg
    14641 tgccgtcccg atacgtgcgg aggcggtcga atccgtggcg gcggttgcgg ctttgctgct
    14701 ggcgcgcgcc cttctgttga atatccactt caaacggcat ccggatttcg gcatcgaaag
    14761 caagcggcgg tttttggttg ccagccgcaa tataacgctg cttttggtgc tgttttcgct
    14821 ggcatttatc tggtcggcgc aaatccaaac gctggctttg tcgatgtttg cggtggcggc
    14881 ggcggtcgtc gtggcgacga aggaactgat tatgtgtctg tcgggcagta ttttaaggtc
    14941 tgccacccag caatactcgg tcggcgacta tatcgaaatc aacggcctgc gcgggcgcgt
    15001 ggtcgacatc aacctgttga acacgctgat gatgcaggtc ggtccgaacc ccttggtcgg
    15061 acagcttgcg ggaaccaccg tttctttccc caacagcctg ttgttgagcc accccgtgcg
    15121 ccgcgacaat attttgggcg actatgtcat ccatacggtc gaaatccccg ttcccatcca
    15181 tttggattcg gatgaagccg tatgccgtct gaaagccgta ctcgagccct tgtgcgcgcc
    15241 ctacatcccc gccatccaac ggcatttgga aaacgtgcag gcggaaaaac tgtttatcac
    15301 gcccgccgcc agaccgcgcg gtacccgcgt gccgtacgat gacaaggcat accgcatcat
    15361 cgtccgcttc gcctcccccg tttcaaagcg gctggaaatc caacaggcgg ttatggacga
    15421 atttttgcgc gtacaatacc gcctgttaaa ttaccccgcc ggctccgaaa cactttaact
    15481 ttccctgacc gaccccattt ccggcttcag acggcatatt gccgatatgc tgtctgaagc
    15541 acaacacgca aaggaaaccc atcttatgac tgacaacgca ctgctccatt tgggcgaaga
    15601 accccgtttt gatcaaatca aaaccgaaga catcaaaccc gccctgcaaa ccgccatcgc
    15661 cgaagcgcgc gaacaaatcg ccgccatcaa agcccaaacg cacaccggct gggcaaacac
    15721 tgtcgaaccc ctgaccggca tcaccgaacg cgtcggcagg atttggggcg tggtgtcgca
    15781 cctcaactcc gtcgccgaca cgcccgaact gcgcgccgcc tacaatgaat taatgcccga
    15841 aatcaccgcc ttctttaccg aaatcggaca agatattgcc ttatacaacc gcttcaaaac
    15901 catcaaaaac tcccccgaat ttgcaacgct ttccaccgca caaaaaacca agctcaacca
    15961 cgacctgcgc gatttcgtcc tcagcggcgc ggaactgccg cccgaacagc aggcagaact
    16021 ggcccaactg caaaccgaag gcgcacaact ctccgccaaa ttctcgcaaa acgtcctaga
    16081 cgcgaccgac gcattcggca tttactttga cgatgccgca ccgcttgccg gcattcccga
    16141 agacgcgctc gcgatgtttg ccgccgccgc gcaaagcgaa ggcaaaacag gctacaaaat
    16201 cggcttgcag attccacact acctcgccgt catccaatac gccgacaacc gcgaactgcg
    16261 cgaacaaatc taccgcgcct acgttacccg cgccagcgaa ctcgcaaacg acggcaaatt
    16321 cgacaacacc gccaacatcg accgcacgct cgcaaacgcc ctgcaaaccg ccaaactgct
    16381 cggcttcaaa aactacgccg aattgtcgct ggcaaccaaa atggcggaca cgcccgaaca
    16441 agttttaaac ttcctgcacg acctcgcccg ccgcgccaaa ccctacgccg aaaaagacct
    16501 cgccgaagtc aaagccttcg cccgcgaaag cctgaacctc gccgatttgc aaccgtggga
    16561 cttgggctac gccagcgaaa aactgcgcga agccaaatac gcgttcagcg aaaccgaagt
    16621 caaaaaatac ttccccgtcg gcaaagtatt aaacggactg ttcgcccaaa tcaaaaaact
    16681 ctacggcatc ggatttaccg aaaaaaccgt ccccgtctgg cacaaagacg tgcgctattt
    16741 tgaattgcaa caaaacggcg aaaccatagg cggcgtttat atggatttgt acgcacgcga
    16801 aggcaaacgc ggcggcgcgt ggatgaacga ctacaaaggt cgccgccgtt tttcagacgg
    16861 cacgctgcaa ctgcccaccg cctacctcgt ctgcaacttc gccccgcccg tcggcggcag
    16921 agaagcgcgt ttgagccacg acgaaatcct catcctcttc cacgaaaccg gacacgggct
    16981 gcaccacctg cttacccaag tggacgaact gggcgtatcc ggcatcaacg gcgtagaatg
    17041 ggacgcggtc gaactgccca gccagtttat ggaaaatttc gtctgggaat acgacgtctt
    17101 ggcacaaatg tccgcccacg aagaaaccgg cgttcccctg ccgaaagaac tcttcgacaa
    17161 aatgctcgcc gccaaaaact tccaacgcgg catgttcctc gtccggcaaa tggagttcgc
    17221 cctctttgat atgatgattt acagcgaaga cgacgaaggc cgtctgaaaa actggcaaca
    17281 ggttttagac agcgtgcgca aagaagtcgc cgtcgtccga ccgcccgaat acaaccgctt
    17341 cgccttgagc ttcggccaca tcttcgccgg cggctactcc gcaggctatt acagctacgc
    17401 atgggcggaa gtattgagcg cggacgcata cgccgccttt gaagaaagcg gcgacattgc
    17461 cgccacaggc aaacgcttct ggcaagaaat cctcgccgtc ggcggatcgc gcagcgcggc
    17521 agaatccttc aaagccttcc gcggacgcga accgagcata gacgcactgc tgagccacag
    17581 cggcttcgac aacgcggctt gatcgatgtg aacaaaaaat gccgtctgaa acaatcaaac
    17641 agtgtttcag acggcatcag catttgcacg gtttcgccat tttcacaaaa acagtaagcc
    17701 aaaatcccgt cattcccgcg caggcgggaa tccagaccgg tcggcacgga aacttatcgg
    17761 gtaaaaaggt ttctccggtc ctgaatccta gattcccgct ttcgcgggaa tgacgcggaa
    17821 aagttgctgt gatttcggat aaattttcgc aacgtttaat ttccgtttta cctgataaat
    17881 gcccgcaatc tcaaatcccg tcattcccca aaaacaaaaa aaatcaaaaa cagaaatccc
    17941 gtcattcccg cgcaggcggg aatccaggtc tgtcggtgcg gaaacttatc ggataaaacg
    18001 gtttctttag attttacgtt ctagattccc gctttcgcgg gaatgacgga atatttttga
    18061 atttgataaa aatgccgtct gaaacggtca aacaacgctt cagacgg
//
LOCUS       DAWFJR010000037        17288 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWFJR010000037 DAWFJR010000000
VERSION     DAWFJR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17288
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.037"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(175..1266)
                     /gene="mltB"
                     /locus_tag="WGR04_001127"
     CDS             complement(175..1266)
                     /gene="mltB"
                     /locus_tag="WGR04_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ0794688.1"
                     /translation="MKNRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVPVSDSGFAVNANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAVIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1175..1427)
                     /locus_tag="WGR04_001128"
                     /pseudo
     CDS             complement(1175..1427)
                     /locus_tag="WGR04_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273930.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1469..2896)
                     /locus_tag="WGR04_001129"
     CDS             complement(1469..2896)
                     /locus_tag="WGR04_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0794689.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKYGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQVAQGLEMIFKGEGGGLGVTEPETSGAAIAREMQSYYEYIDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLGRYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AKHEDAAACLLNDIRKDILDCRYCG"
     gene            3081..6779
                     /gene="mfd"
                     /locus_tag="WGR04_001130"
     CDS             3081..6779
                     /gene="mfd"
                     /locus_tag="WGR04_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ0794690.1"
                     /translation="MTYPIPKPREKSRWSNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTETQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSSDVFAGRLKNYGQVLP
                     DIFGVEHTLPNVAVNRQADEPLQALKDFQTAFKGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAAVVSDSLKAAAVSTESSLPPGKSNLHGQIQQQPAPSP
                     VGEGWGEGKAVAAQSAIAVITESDLYQYVARSRIHNRRKKHAAVSDDLLRDLAEINIG
                     DPVVHEEHGIGRYMGLITMNLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHE
                     NIALHKLGSGAWNKAKRKAAEKARDTAAELLNLYARRAAQSGHKFEINELDYQAFADG
                     FGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAV
                     LAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKAALEGMVDGTVDIVIGTHK
                     LVQDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGL
                     RDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRER
                     LETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIIN
                     RADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLA
                     MQDLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEI
                     KLHSPALLPESYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIE
                     SHHLRLMAKELGIDAIDAAGEAVTVTFGKNNNVDPTEIILLIQNDKKYRLAGADKLRF
                     TAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            6928..7311
                     /locus_tag="WGR04_001131"
     CDS             6928..7311
                     /locus_tag="WGR04_001131"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ0794691.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            7333..8175
                     /gene="kdsA"
                     /locus_tag="WGR04_001132"
     CDS             7333..8175
                     /gene="kdsA"
                     /locus_tag="WGR04_001132"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ0794692.1"
                     /translation="MDIKINGITVGNDAPFVLFGGINVLEDLDSTLQTCAHYAEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFREAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            8190..8630
                     /locus_tag="WGR04_001133"
     CDS             8190..8630
                     /locus_tag="WGR04_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ0794693.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIAALVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAILTRLQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSMIFMATALLSAVLSWRLSGKDAV"
     gene            8678..9964
                     /gene="eno"
                     /locus_tag="WGR04_001134"
     CDS             8678..9964
                     /gene="eno"
                     /locus_tag="WGR04_001134"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ0794694.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGNKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIIPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKASEDVLFALDCASSEFYKDGKYHLEAEGRSYSSAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAAYYPGKAAFYQLDK"
     gene            9979..10257
                     /gene="ftsB"
                     /locus_tag="WGR04_001135"
     CDS             9979..10257
                     /gene="ftsB"
                     /locus_tag="WGR04_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ0794695.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(10298..10588)
                     /locus_tag="WGR04_001136"
     CDS             complement(10298..10588)
                     /locus_tag="WGR04_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ0794696.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
     gene            complement(10709..11842)
                     /gene="nrdB"
                     /locus_tag="WGR04_001137"
     CDS             complement(10709..11842)
                     /gene="nrdB"
                     /locus_tag="WGR04_001137"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ0794697.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(11903..13090)
                     /locus_tag="WGR04_001138"
     CDS             complement(11903..13090)
                     /locus_tag="WGR04_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction enzyme"
                     /protein_id="HEZ0794698.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            complement(13083..14096)
                     /gene="dcm"
                     /locus_tag="WGR04_001139"
     CDS             complement(13083..14096)
                     /gene="dcm"
                     /locus_tag="WGR04_001139"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0794699.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            complement(14154..16433)
                     /gene="nrdA"
                     /locus_tag="WGR04_001140"
     CDS             complement(14154..16433)
                     /gene="nrdA"
                     /locus_tag="WGR04_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ0794700.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKVAYGEYEPP
                     HLYDHVKKLTDAAKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPRLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPCVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFKQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRAEIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            16962..17273
                     /locus_tag="WGR04_001141"
     CDS             16962..17273
                     /locus_tag="WGR04_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ0794701.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGRFR
                     R"
BASE COUNT         4332 a         4552 c         4116 g         4288 t
ORIGIN      
        1 gtactggttt aaatttaatc cactatacaa cggaatcgat tgcaaatctt tcccactgtt
       61 cttgaaagaa tgcgaatttc gatttaactt cggcacaccg tctcaacagc ttaaaatcct
      121 gcgggattgg tgtgggattt agggctaatc tagtacagcc ccttttaaaa aacattacaa
      181 tcccgggccg ccaagcgaat tggcaatgtc cctgaccgcc gttacataca tccgactgtg
      241 attgtactgc cataccgtat aaaaattgtt caagcccaaa taatattcaa acacgcctgg
      301 tgcggtttcc agtttgaaca aaaccgcctt ttcatcatcg gcaagctctt cgccggggat
      361 gatgccgtac gccttcaaat ccgccaccgt ccgcgtcagg gcggtttttt cgccaatgat
      421 tgcctgaaca tccgcacccg gcgccaatgt tgcagacacc agtattttcc cgcccgtgcg
      481 ccaaccgtgc tgcttcatat aattggcaac cgatgccgcg acatcgccga tgttgcccca
      541 tatgtcccga tgtccgtccc cgtcataatc caccgcccat ttccggtagc tcgaaggcat
      601 aaattgcggc atccccattg cgcccgcata gctgccttta aaggcgaaaa catcgccgcc
      661 ttcttctttt gccagcttta aaagctcgac caattctttt tggaaaaacc cggcgcggcg
      721 ggggtaatca aagcctaagg tcgccaatgc gtccgccaca cggaaactgc ccgtattttt
      781 accgtaattc gtttcaatcc cgataaccgc cacgataagt tcggcaggca cgccgtattt
      841 ttgcgccaca tcatcgataa gtgcgcggtt ttccgcataa aaccggcgcg cgccgcgaaa
      901 tttcgccttg cccgaatttc ccgtgcggaa cacataccac ggacgcgatg tggaggggcg
      961 gtgcataatc ttgacgatgt ccgccttgta agccgctttg tcaaaaaaat cctgccattc
     1021 cgcccgggaa aaatcccctt tcccgacttc atcgtccaca aaacggcgga catttgcatt
     1081 gacggcaaac ccgctgtcgg ataccggtac ggctgccgcg tcaaacgcgg ggcggctttc
     1141 ttttttcatt tcatccgcgc ggggggcttg ggcttcattt gcccggggcg ggcgtgcctc
     1201 catcgccgta caggcagaca aagccgccaa acaaattgcc agcggcagta tttttctgtt
     1261 tttcataaat atgttccgaa caaatagggt aagtgggaaa gcggcgcaag gggcgcggcg
     1321 cgaaatgcgg catccgccgc aatcggcggc tttgcggaat gccgcacgtt gccttttacg
     1381 ccgcctgaaa tccgtatgtc gtcgccgaaa atgccgtctg aaggcacttc ccccttcaga
     1441 cggcattgcc tgccgccgta tttgcccgct atccgcaata tcggcagtcc aatatgtctt
     1501 tgcggatgtc gttcagcagg caggctgccg cgtcttcgtg tttcgcctgt acgaagacaa
     1561 agagtcgggc gatgattttg ccgataaaga ctttttgcag ggcgcaggca tcggtcaggg
     1621 tgtgttcctg caggaagctg cggatgtctt caggcaaagt gtagtcgcgg gcgacggcgg
     1681 caaagcgggc atcggtttgc aggcggtcga gcagggtttg gtttttgtcc aacttcatgc
     1741 ctgcttcttt ttcttcggcg gtggcgcgga caaactggtc gtaaatttcg gcataaagca
     1801 tatcgttcgg gccttcaaaa atcgtgaagg gacggatgtc gatggcgata ttgccggcgg
     1861 tgtgtccgcg ttcaaaaccc ttcgcgccca agagtttttg caacatttgc gcggcggcat
     1921 aagtgtattc cgtggcgagg gttttgacga tgttcgcctc catcagctga tgggcgacgg
     1981 gggcaacagg cgaaacggaa tggcagacgt agcggtaaag aatctcggaa acctgatggc
     2041 ggcgccggat ttcgcggcgt tcgtaatcga cgaatctgat gtcgttgcgg acgtatcgtc
     2101 ccaggttttc aaggatgtat tccataatgc cgtgcgtcat gccgatcagt tgcaggcggc
     2161 tgcggataaa gatgttttgg aacgcgcgca aaccggcagc gtcgctctgg gagagtttca
     2221 tcacggcggt tgcaggcatt tcggcatcga tgcggttgac ggcgtaacgg acggcgcgca
     2281 agccttcgga tgcgagggtt tcgcagcgga tgtatgtttt ggggacgagc agcaggtcga
     2341 tgactttggc gagtttgccg tttttgcgct ctttggcggc aacgaggagg aagtcgcttt
     2401 gcgagttgcc ctgccagtat ttcgcggcgt tgacgtaaat ggtttgtccg tcgatatatt
     2461 cgtagtagga ctgcatttcg cgtgcaatcg ccgcgccgga ggtttcgggt tcggtaacac
     2521 ccaaaccgcc gccctcgcct ttgaaaatca tctccaaacc ttgcgcgact tgcgcttcat
     2581 cgccgaactc ttgcagtggc tgcaacacca gcgcgccttc gatgccggta cgcagcgtaa
     2641 cgggcacgcc gtaatgcccc gcaatccgca ggacttcttg gatttcaaac tggctgccct
     2701 tgcgcccgcc gtattttttg tcgaggaagg gcaacagcaa acccgcctgc ttcaaggcaa
     2761 gccatttgtc ttcgggcagg tatcgcatca ggtcgatacc gtctgaaaaa atgcggcgga
     2821 atgcggattc gatgtgcttt aaaaaagcag ccgtgtccat agttgacggc tgcgcgctcg
     2881 gttcggtgtg tatcatcggc ttcctctgtc ggttcccatt aatcggcggc cggtcaaacc
     2941 gcctgccaca gtttagagtt gattttctaa actttaccac aaagtgcgcc gggcaacaat
     3001 ccgccgacct ttcagacggc atcgcgcccc ctcccgtgct aaaatgaccg tttgcatcac
     3061 tgtccgccga ttgccgcact atgacctacc ccatccccaa accccgtgaa aaatcccgtt
     3121 ggtccaatct ttcgcaaggc tcgctgccct tggctttggc gcgttatctg ccgcacaagc
     3181 ggctcaaggt cgtgctgacc caagatgcgg aacaggcgtt gcgccttcag acggcatggc
     3241 ggtttttccg tccgcacgat acggcggtgt tcctgccgga ctgggaaacg ctaccttacg
     3301 agcgtttttc tccgcatcag gatttggtgt cggagcggct gtcggcgttg tggcagatta
     3361 agagcggcgc ggcggacgtg ttgtttgtgc cggttgccac ggcgatgcag aagcttccgc
     3421 ccgtgccgtt tttggcgggg cgcacgtttt ggctgaaaac aggacagact ttggatatag
     3481 gccgtctgaa aaccgatttg gtagatgcgg gctacaacca tgtttcccat gttgttgcgg
     3541 cgggcgagtt tgccgtgcgc ggcggcattg tcgatttgtt ccccatgggc agcgaaatgc
     3601 cctaccgcat cgatttgttc gacgatgaaa tcgacagcat caaaaccttt gatactgaaa
     3661 cgcagcgcac catttctcct gtgtctgaaa tccgcctgct gccggcacac gagttcccca
     3721 ccgacagcga agcgcaaaaa atcttccgca gccgcttccg agaagaagtc gacggtaatc
     3781 cgaacgatgc ggctgtgtac aaagccgtca gcaacggcca cttcggcgcg ggcgtggaat
     3841 attatctgcc gctgtttttt gaaaatgagc tggaaacgct gtttgactat atcggcgaag
     3901 acgcgctgtt tgtctctttg ggcgatgttc acgccgaggc aaaccgcttt tggagcgacg
     3961 ttaaatcacg ttacgccatg gcgcagggcg acgaaaccta tccgcctttg cttccacagc
     4021 atttgtatct ctcctcagat gtttttgcag gccgtctgaa aaattatggt caggtattgc
     4081 cggatatttt tggtgttgaa catacgttgc caaacgtggc cgtcaaccgc caagcggatg
     4141 aaccgttgca ggcattgaaa gattttcaga cggcctttaa aggacggatt ttgttgtgcg
     4201 ccgaaagttt gggacgacgc gaaaccatgc tcggtttctt gcagcaaaac ggtttgaaag
     4261 ccaaacccgt gtccgactgg cagggctttt tatcggcaca cgagccgctg atgattacag
     4321 tggcgccgtt ggcatacggg ttcaaactgg gcggactgca atcgccgaac caacagcaaa
     4381 ctacttccgc ctccgaggga gaaggcgatg cggttaccga ccaaaccgaa ttttccgcat
     4441 ccgcaacaaa ccctctccct agccctctcc ctcaggagag ggaacaaagt gcagccgtcg
     4501 tttcagacag tctgaaagca gccgccgttt caaccgaaag cagcctgccc cccggtaaaa
     4561 gtaatctgca cgggcaaatc caacagcaac ctgccccctc ccccgtgggg gagggttggg
     4621 gagaaggcaa agcagttgcc gctcaaagtg ccatcgccgt catcaccgaa tccgatctct
     4681 accaatacgt cgcccgttcg cgcatccaca accgccgcaa gaaacacgcc gccgtttcag
     4741 acgacctgtt gcgcgacctt gccgaaatca acatcggcga ccccgtcgtg cacgaggaac
     4801 acggcatcgg ccgatatatg ggcttgataa caatgaactt gggcggcgaa accaacgaaa
     4861 tgatgttgct cgaatatgcg ggcgaagcgc agctttatgt gcctgtttca caactgcatt
     4921 taatcagccg ctactccggt caggcgcatg aaaacattgc cctgcacaag ctcggcagtg
     4981 gcgcgtggaa caaggcgaag cgcaaagccg ccgaaaaggc gcgcgatacc gccgccgagt
     5041 tgcttaacct ttacgcccga cgcgctgccc aatcgggaca caagtttgaa atcaacgagt
     5101 tggattatca ggcgtttgct gacggcttcg gctacgagga aaccgaagac caggccgccg
     5161 ccatcgccgc cgtgattaaa gatttgacgc aagcgaagcc gatggatcgc cttgtgtgcg
     5221 gcgatgtcgg tttcggcaaa accgaagtcg ccctgcgcgc cgcgtttgtg gcggtgatgg
     5281 gtggcaaaca ggtcgccgta ctcgccccaa ccacgcttct ggtcgagcag cacgcgcaaa
     5341 actttgccga ccgtttcgcc gatttccccg tgaaagtcgc cagcctttcg cgtttcaaca
     5401 acagcaaagc caccaaagcc gcgctcgaag gcatggtgga cggcacggtc gatattgtta
     5461 tcggcacgca caaattggtg caggacgaca tcaaattcaa aaacttaggt ttagtgatta
     5521 tcgatgaaga acaccgcttc ggcgtgcgtc agaaagaaca gctcaaacgc ctgcgcgcca
     5581 atgtcgacat ccttaccatg accgccacgc cgattccgcg caccctcagc atggcactcg
     5641 aaggactgcg cgacttttcg ctgattacca ccgcgcccag ccgccgcctt gccgtcaaaa
     5701 cctttgtcaa accctttagc gaaggcagtg tgcgcgaagc agtattgcgc gaactcaaac
     5761 gcggcgggca agtgtttttc ctgcataatg aagtggatac catcgaaaac atgcgcgagc
     5821 ggctggaaac cctattgccc gaagcccgca tcggcgtggc gcacggacaa ctgcgcgagc
     5881 gcgagctgga gcaagtgatg cgcgactttt tgcagcaacg atttaacgtg ttgctctgtt
     5941 ccaccatcat cgaaaccggc atcgacatcc ccaacgccaa taccatcatc atcaaccgcg
     6001 ccgataagtt cgggctggcg caattgcacc agcttcgcgg gcgcgtcggc cgcagccacc
     6061 accaagccta cgcctacctg ctcacgcccg aatacatcac caaagacgca gaaaaacgtc
     6121 tcgatgccat cgcggcggca gacgaactcg gcgcaggttt caccctcgcc atgcaggatt
     6181 tggaaatccg cggcgcaggc gaaatcctcg gcgaagggca gtccggcgaa atgatacagg
     6241 tcggcttcac gctctacacc gaaatgctca aacaagccgt gcgcgacctc aaaaaaggcc
     6301 gccagcccga cctcgacgca ccgctgggca tcaccaccga aatcaaactg cacagccccg
     6361 ctctgctgcc cgaaagctac tgccccgaca tccacgagcg tctcgtcctc tacaaacgcc
     6421 tcgccgtctg cgaaaccgtg cagcaaatca acgccataca cgaagaactc gtcgaccgct
     6481 tcggactgcc cgaacaaccc gtcaaaaccc ttatcgaaag ccaccacctg cggcttatgg
     6541 caaaagaatt gggtatcgat gccattgatg cggccggcga agcggtaacg gtaacctttg
     6601 gtaaaaacaa taatgtcgat ccaaccgaaa tcatcctgct gattcagaac gacaaaaaat
     6661 accgccttgc cggcgccgat aagctgcggt ttaccgcaga gatggaaaat atcgaggtca
     6721 gaatcaacac cgtaaaaaac gttttaaaaa ccttgcaaaa cagatgcctg cccaaataaa
     6781 gccgacaccg caatgccgtc tgaaacaccg ttttccttgt ccgaaagccg ccattatgaa
     6841 tttgaaggaa actccactat aatacggcat tcagatttcc agacggcatc gcgcccgtca
     6901 aaccgcacac aaaccaaaag gaaatacatg ttccgtacta tgcttggcgg aaaaatccac
     6961 cgcgccaccg ttaccgaagc cgatttaaac tatgtcggca gcattaccgt cgatcaagac
     7021 ctgttagacg cggcaggcat ctaccccaac gaaaaagtcg ccatcgtcaa caacaacaac
     7081 ggcgaacgtt ttgaaaccta taccattgca gggaaacgcg gcagcggcgt gatttgtctg
     7141 aacggtgctg cggcaaggct ggtgcagaaa ggcgatatcg tcatcatcat gtcttacgtc
     7201 caactctccg aacccgaaat cgccgcgcat gaacccaaag tcgtcttggt agacggaaac
     7261 aacaaaatcc gcgacatcat ctcctacgag ccgccgcaca ccgtgctgta accctgaaaa
     7321 cggacattga ccatggatat taaaattaac ggcattaccg tcggtaacga cgctcctttt
     7381 gtactgttcg gcggcatcaa cgttttagaa gatttggatt ccaccctcca aacctgcgca
     7441 cattacgccg aagttacccg aaaactcggt attccctata tctttaaagc ctctttcgac
     7501 aaggcaaacc gttcctccat ccattcttat cgcggcgtag gcttggaaga aggcttaaag
     7561 atttttgaaa aagtcaaagc agagttcggc atccccgtca ttaccgacgt acacgaaccc
     7621 catcagtgcc aacccgtcgc cgaagtgtgc gatgtcatcc agcttcccgc ctttcttgcg
     7681 cggcagaccg atttggtggc cgcaatggcg gaaactggca atgtcatcaa catcaaaaaa
     7741 ccccagttcc tcagcccctc tcaaatgaaa aacatcgtgg aaaaattccg cgaagccggc
     7801 aacgggaagc tgattttatg cgaacgcggc agcagcttcg gctacgacaa cctcgttgtc
     7861 gatatgctcg gtttcggcgt gatgaaacaa acctgcggca acctgccggt tattttcgac
     7921 gttacccatt ccctgcaaac ccgcgatgcc ggttctgccg catccggcgg tcgtcgcgca
     7981 caggctttgg atttggcact tgcaggcatg gcaacccgcc ttgccggcct gttcctcgaa
     8041 tcgcaccccg atccgaaact ggcaaaatgc gacggcccca gcgcgctgcc gttgcacctt
     8101 ttagaagatt ttttaatccg catcaaagca ttggacgatt taatcaaatc acaaccgatt
     8161 ttaacaatcg agtaacacgg tttcgcctta tgatgcagac tttccgaaaa atcagcctgt
     8221 atgccgcaac cttgtggctc ggtatgcaga ttatggcggg ttatatcgcc gcactggtgc
     8281 tgttcaaaat gctgcccaaa atgcaggcgg gcgaaattgc cggcgtattg ttcgacatcc
     8341 tctcttggag cgggcttgcc gtttggggcg cggtgttggc tgctgccttt gccatcctga
     8401 cccgactgca aaccgccctg ctgctttttt tattgtccgc ccttgccgcc aaccaatttt
     8461 tggttacccc cgttatcgag gcactgaaat acgggcatga aaattggctg ttgtcgtttg
     8521 taggcggatc cttcggaatg tggcacggta tttccagcat gattttcatg gcaaccgccc
     8581 tactttcagc agttttaagt tggcggcttt ccggcaaaga tgccgtctga agccctccca
     8641 tttttttacc tcccttcact tcacttggag aacattcatg agcgcaatcg ttgatatttt
     8701 cgcccgcgaa attttggact cacgcggcaa ccccacagtc gagtgtgatg tattgctcga
     8761 atccggcgta atgggacgcg cagccgtacc gagcggcgcg tccaccggtc aaaaagaggc
     8821 tttggaactt cgcgacggca acaaatcccg ctattccggc aaaggcgtat tgaaggcggt
     8881 cgaacacgtc aacaaccaaa tcgcccaagc cctcatcggt atcgatgcca acgagcaatc
     8941 ttatatcgac caaatcatga tcgaattgga cggtactgaa aacaaaggca atttgggtgc
     9001 gaatgcgact ttggcggttt ctatggcggt tgcacgcgcc gctgccgaag actcaggcct
     9061 gccgctttac cgctacttgg gcggcgcagg cccgatgtcc ctgcccgtac cgatgatgaa
     9121 cgtcatcaac ggcggcgaac acgccaacaa cagcctgaac atccaagagt ttatgattat
     9181 acccgtcggc gcaaaatctt tccgcgaagc gttgcgctgc ggtgcggaaa ttttccacgc
     9241 cttgaaaaaa ctgtgcgaca gcaaaggctt cccgaccaca gtcggcgacg aaggcggttt
     9301 cgcccccaac ctgaacagcc acaaagaagc cctgcaactg atggtcgagg cgaccgaagc
     9361 cgccggctac aaagcgagcg aagacgtatt attcgcattg gactgcgcgt ccagcgaatt
     9421 ctacaaagac ggtaaatacc acttggaagc cgaaggccgc tcctacagca gcgcagaatt
     9481 tgccgaatat ctggaaggtc tggtcaacga gttccccatc atctccatcg aagacggcat
     9541 ggatgaaaac gactgggaag gctggaaact gctgaccgaa aaactgggcg gcaaagtcca
     9601 actcgttggc gacgacctct tcgttaccaa cccgaaaatc cttgccgaag gcattgaaaa
     9661 aggcgtggca aacgcactat tggtcaaagt caaccaaatc ggtactttga gtgaaaccct
     9721 gaaagccgtc gatctggcaa aacgcaaccg ctacgccagc gtgatgagcc accgctccgg
     9781 cgaaaccgaa gacagcacca ttgccgactt ggcagtcgcc accaactgta tgcagatcaa
     9841 aaccggttct ttgagccgtt ccgaccgcat ggcgaaatac aaccaactgc tgcgtatcga
     9901 ggaagaattg gcggaagctg cctactaccc cggcaaagcc gcattctacc aactggacaa
     9961 ataagaaaag gttaaggtat gaagtgggta actgtcgttt tatccttcgc acttgtctgt
    10021 tgccaataca gcctctggtt cggcaaaggc agcatcggac gcaacagcag tctgagagaa
    10081 cagattgccg ttcaagaaga aaaaaaccag acactcgccc tacgcaatca ttcccttgcc
    10141 gccgaagtct atgatttgga aaacggtcaa gaagccattt cggaaatcgc ccgggtagaa
    10201 ctgggttata tccaagacgg tgaaaccttt taccgactca tcaggcataa ccggtaatac
    10261 cgtcaaaaag ccgtccgaac caatgttcgg acggctttta tttcaacaaa ctgtcagaca
    10321 gcccctcatc ctcccccgac aaaccgcaat ccagcctgac atccccctcg acgcaacagc
    10381 agcacggcag tatctcgtcc cgccccaaaa aagccaaagg cggctcccga taagtaacgc
    10441 ttccctccaa aatcttcact cggcacgatc cgcaatatcc gcttcggcac tgatattcca
    10501 ccatatgccc cgtccgttcc aagccttcca acagagtctc gccctccaag agttcaaaaa
    10561 aacccttatt cgtaccaatg cgcgccattt ccgaccaatc aaaaataaaa ggaacaataa
    10621 ccgatcctac ccgctgtttt tcccatcata caacatacaa atgccgtctg aaacatccgg
    10681 cttcagacgg cattttttca aaaaacattt acaactcaaa atcgcccaaa tcatccgtat
    10741 tcacttcaga atcgatctga ccgatcaaat aagaggatat ttccacttcc tgcggcgcga
    10801 cctgtacgtt gtcggacgac agccacgcat taatccacgg aatcgggttt tgatttgcgc
    10861 cttcaaatcc ggccggcaac cccaccgcct gcatacgcag attggtaata tattcgacgt
    10921 attgggataa gatttctttg ttcaaaccga tcatcgaacc gtctttaaac aaatatgccg
    10981 cccattcttt ttcctgttcc gccgcttttt tgaagagttg gaaacattcg tcctgcaact
    11041 cggcggcaat ttccgccatt tcagaatcat caacacccga acgcatcaga ttaagcatat
    11101 gctgcgtgcc ggtcaggtgc agggcttcgt cgcgggcaat cagtttgatg attttggcgt
    11161 tgccttccat caactcgcgc tcggcaaaag caaacgagca ggcgaatgaa acgtagaaac
    11221 ggatggcttc caacacgttg acgcacatca ggcagagata gagttttttc ttcaacccgc
    11281 gcaaagacac agtaatgggt tttccgccga cattgtgcac cccttcgccc aacagattgt
    11341 aatactgggt gtattcgatt aagtcgtcgt aatagcaggc aatgtcttcg gcgcgggcgg
    11401 taatgtattc gttttcgaca atatcatcaa acacgaccga cggatcattc acaatattgc
    11461 ggatgatgtg ggtatagctg cgcgagtgta tggtttcgct gaagctccac gtttcaatcc
    11521 acgtttccaa ctcgggaatc gaaaccaaag gcagcaaggc aacattcgga ctgcgccctt
    11581 ggatggaatc gagcagtgtt tggtatttca gattgctgat gaaaatatgt ttttcgtgtt
    11641 cgggcagatt ggcgtagtcg atacggtcgc gcgagacatc gatttcttca ggccgccaga
    11701 agaaagacaa ttgtttttca atcagttttt caaatacttc gtatttctgc tggtcataac
    11761 gggcaacatt aaccggctga ccaaaaaaca tcggctcatt cagtgcgtcg tttttggttt
    11821 tgggaaaggt gctgtatgac atagttaagt gttcgcagga cataatctat tctctatatt
    11881 aattaaaaat gatctgtaag ttttaactaa cctgctgaga ctttatatat ttaataaaat
    11941 tttctaaagt taaaacatgg gttttcccaa aagttccaaa tgtttttacg gattctttag
    12001 ggacaactaa ggttaaattc tcatcactca tttcctgaat ttgagtatca gatatacctt
    12061 cttgtagagt aaataaatgc ttatgaggaa ttcgatctgc ttcatttaaa acttggcgcc
    12121 agcgatcctt acaagtagtc tttgaaccta gcattgtcag cttttcagtt tgaaatatga
    12181 aattccctga ttcatccatc gcatggtact cttcacttcc aggaaataaa aaatctggtt
    12241 tctttttccc ttctgtcttc gcttgcgtct caaattttaa ggaaaattcg tcaaatattc
    12301 ttgataaatg taactctaaa gattttcctg ctcttgcttt tctgcgattt gtaaaagaat
    12361 gtgcaaaatt aatgaagcta tccaaatcag taatttttga tttaataatt tcaaattcac
    12421 gtttttcaaa aatggaaaat aattcatatt cctttgaaac ccattgtttt aacacattac
    12481 taacgttagt tttatttaat gcaatatttt cccttgccaa aactgccatt tcttcagttt
    12541 tggggaagtt aggaaatagt gaaaataatt ttgttaggtt atcctctact tcttgttttt
    12601 taggtaaata taaacttcct ggtaaaatgt tagtttctgc gataaatatt tcaatatctt
    12661 catctgaaga taaaacaaaa gcatggaata ataaatcttt ataacaagct ctgcacaata
    12721 ccaataagga accactatat ttatcactta aaaattcaaa attcttgcca aaacccgtaa
    12781 ttctcgcctc atttctcgtg ccttggccgt aatatttaaa attgctatta gtgacgcttc
    12841 catcttgcca attaattttg attgaaatag ttttatttgt tcccttttgg caaactacat
    12901 caaagaggtc gtctgaaaaa tgtttttgaa tataaaatcc tgattggtga ctaccagtag
    12961 taccaacatc attgggacga atataacgac agtatactgc aattgattta tttgctgtct
    13021 gtattgctga agctactaag tcatcggatt tatttactac ttgtattgtc tgagctgcca
    13081 agtcattcat tttttaactc atttataatt tgtttagcaa tagcttgaac caacggtaca
    13141 gcaattgaat tgccaaactg cttgtatgca gctgtcttgg atactgcatc aataacaaaa
    13201 tctttaggaa atcccattaa acgcgagcac tccctaggtg tcagcttcct aggatttttt
    13261 cctttctgag ggatgagtat ttcggaacca tctttgtaat atcgtgcaga tagagttcgt
    13321 gatattccat ctaaatcaac taatccaaaa ccaaatccat taccctttgc cttatgtttt
    13381 ttagcgtaat tttgaaggta aagccataag ttatcagaaa gagtaaaaga attatctaca
    13441 tcatcttcca aaatttgctt taattttggt tgtgattctg gtggggaagg aaaattgaaa
    13501 tttatttcct tattaaaata ttgtctatca aaacctacaa taaaaatacg ctccctattt
    13561 tgaggaacat aatattttgc attcataact tgataaaata tctgatagtc aagctcttct
    13621 aaagtccctt taattacttt aaatgtattt cctttgtcat gcgaaacaag gtttttcaca
    13681 ttctctaaaa gaaaaatttt aggtcgatgt tttccaataa tttcagcaac atcaaaaaat
    13741 agagttccct gcgccttatc taagaagcct gtttctcgtc ctaggctttt tttctttgaa
    13801 acaccagcta tagagaatgg ctgacacggg aatcctgctg ttaatacatc aaacttactt
    13861 ggaatagctg ctttggtttc ctttaatgta atatctccat aaggaatatc attaaaattt
    13921 acttggtagg tttgacgggc tttatcatcc cattcactag aaaatacaca tcgcccacca
    13981 acattctcca ttgcaatgcg aaaaccacct attcccgcaa ataaatcaat aaaagtaaat
    14041 ttctcattat ttaaggtaac tatattatct aattgatttt gtattttatt tttcatattt
    14101 tatatttcag atgatttatt aatctaaagg ccatctgaaa actccccctt tcatcaaatc
    14161 ttacaagccc cgcccgcgca gccgtcatcc tgaatatcgg tctgcgtatc gtccgcaccg
    14221 tcgcgggtgt tatggtagta cagggttttg acgccgtatt tgtaggcggt cagcaggtct
    14281 ttgagcattt gtttcataga aactttgccg ccttcgaatt tgcccgggtc gtaggcggta
    14341 ttggcggaaa tcgattgatc gacgaatttt tgcatcacgc cgacaagttt caggtagcct
    14401 tcgttgccgg gaagctgcca cagggtttca taggcatttt tcagggtttc aaactccggc
    14461 acgacttgtt tcaaaatgcc gtctttcgat gctttgaccg ttaccaatcc gcgcggcggc
    14521 tcgatgccgt tggtggcgtt ggcgatttga gagctggttt cagacggcat gagcgcggtc
    14581 agagtagagt tgcgcaggcc gtatttgacg atttcggcac gcaggctttc ccagtcgtaa
    14641 tgcaaaggct cgccgcagac ggcatccaaa tcttttttgt aggtgtcgat gggcagtttg
    14701 ccttgcgaat aaacggtttg cttaaagagc gtacacgcac cgtattcttt ggcaaggttt
    14761 gccgatgctt tgagcaggta atactgtatg gcttcaaagg tacggtgggt cagaccgagc
    14821 gcggagccgt cgctgtagcg gacaccgttt ttcgccagat aataagcata gttaatcacg
    14881 ccgatgccga gcgaacggcg gcccatagta gaggtacgcg cggcttctac cggatatccc
    14941 tgataatcta aaagtgcatc gagcgcacga acggtcaagt cggcaagccc ttccaattcg
    15001 tccaagctgt ttaatgcgcc caagttaaag gcagacagtg tacacagggc gatttcgccg
    15061 ttcggatcgt tgatattgtc cagcggtttg gtcggcaggg cgatttccat acacaagttg
    15121 gactgatgaa caggcgcgac gcacggatcg aacgggctgt gcgtattgca gtgatcgacg
    15181 ttttgaatgt agatgcgccc ggtcccggca cgctcctgca tcagcgtgga aaacaggtcg
    15241 gcagccggaa tgatacgctt gcggatatca gggtcttgct cgtatttcgt atagagccgc
    15301 tcaaattcgt cttggtcggc aaaaaacgct tcgtacaacc tcggaacctc gttgggcgaa
    15361 aacagcgtaa tgttgccgcc cttaatcagg cgggtgtaca gcaggcggtt gatttgcacg
    15421 ccgtaatcaa gctgacggat acggttgtct tccacaccgc ggttgttttt caacaccagc
    15481 aggctttcgg cttcgatatg ccacaagggg tagaacaaag ttgccgctcc gccgcgcacg
    15541 ccgccttgcg aacaggattt gaccgccgcc tgaaacattt taaagaaggg aatgcagccg
    15601 gtatgccgcg cttcgccgcc ccggatttcg ctgtccaaac cgcggatacg tccggcattg
    15661 atgccgatgc ccgcacgctg ggaaacgtat ttcacaatcg cgctggtagt ggcattgatg
    15721 gaatccaagc tgtcgtcgca ttcaatcagc acacagcttg agaactggcg cgtaggcgta
    15781 cgcacgccgc tcataatcgg ggtcggcagc gatactttaa atgtggaaac ggcatcgtaa
    15841 aaccgtttga cgtaatcgag gcgtgtttct ttgggatatt tgctgaaaag acacatcgcc
    15901 accagaacat ataagaactg cggtgtttca taaatctggc gggtaacgcg gttctgtacc
    15961 agatatttgc cttcgagctg tttgacagcg gcataggaaa aggacatatc gcgttcgtgg
    16021 tcgatatagg cgttcagctc gtcaaattct tcgcggctgt aatcctcaag gatatgcctg
    16081 tcgtattttg cggcatcggt aagtttttta acgtggtcgt aaaggtgcgg cggctcgtac
    16141 tcgccgtagg ctactttacg cagatggaaa atcgccaaac gcgcggcaag gtattggtag
    16201 tccggggtat cttccgaaat taaatcggca gcggctttga tgatggtttc gtggatgtcg
    16261 tcggtgcgga tgccgttgta gaactggatg tgcgatttca actcgacctg agatacggaa
    16321 acattttcca atccgtccgc cgcccaagtg acgacacggt gaatcttatc caaatcaatg
    16381 gcttctaatc ttccgtctcg tttggttact ttcaaatcag tcggtgtatt catcgcttcc
    16441 tcttccactc ttgatattca agacacagtc ttttcaaata aattaaggca gacaatatag
    16501 tggatttttg gcattttctc cagtcttgac aacggttgta tttttcagtt tcaggcaatg
    16561 cgcgataaaa cccgcctgtc gtatttttcc tataatattt gttttatctg ataattcttt
    16621 accgataaaa aacgggtaaa ttttttgcct tttgaccggc tccggctaca aggcggtgaa
    16681 ataaggattt tccgacgaaa aaggaaagct tcctgttttc tgcccctgca aattgttaaa
    16741 ttttgcaaag tatgattttg ccgcgccgcc gccgacaaat tccattttct taccgattgg
    16801 aatttattat tgagattaat gtgttatttg aatctgcata tcaaacggca agttttgtcg
    16861 gctgaatgag tctgaaactg ccgacaattt gcccgttcca tccttccatc ctatactgga
    16921 aagaatgaca aactgaaagg atcgtcatgt ctgtcaaaaa gatgcttgcc atactgttgt
    16981 ctgcaatatt gggactggta tcgacaactg ccgctgccgg tacgtcagaa cccgcccacc
    17041 gcgataccaa acatatccgc aaggcaaaca agcagatgct gcaccccgaa tgcaggaaat
    17101 atttggaacg ccgtgccgcg tggtaccgat cgcaaggcaa cgtgcaggaa ttgcgcgaaa
    17161 acaaaaaggc gcgcaaagca ttccgctccc tgccttatgc ggaacagaaa atccaatgcc
    17221 gggcggctta tgaggctttc gatgatttcg acggcggcag gttccgccgt taatcccata
    17281 taaaatat
//
LOCUS       DAWFJR010000038        16809 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWFJR010000038 DAWFJR010000000
VERSION     DAWFJR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16809
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.038"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(27..653)
                     /locus_tag="WGR04_001142"
     CDS             complement(27..653)
                     /locus_tag="WGR04_001142"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ0794702.1"
                     /translation="MRKIRTKICGITTPEDASAAVAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDTFCRQFDRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATTNRLSR"
     gene            complement(712..1203)
                     /gene="greB"
                     /locus_tag="WGR04_001143"
     CDS             complement(712..1203)
                     /gene="greB"
                     /locus_tag="WGR04_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ0794703.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            complement(1303..2847)
                     /gene="purF"
                     /locus_tag="WGR04_001144"
     CDS             complement(1303..2847)
                     /gene="purF"
                     /locus_tag="WGR04_001144"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ0794704.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLVGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRRGVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDNEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDDIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            complement(3056..3553)
                     /locus_tag="WGR04_001145"
     CDS             complement(3056..3553)
                     /locus_tag="WGR04_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ0794705.1"
                     /translation="MNNLPVADLLVFAIIAACIVISTMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKILRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGILIITLLVMLASKTDLPDTEEWRQSYTIPFFVSLSEAVLKHADDRDG
                     ALEDD"
     gene            complement(3546..4544)
                     /gene="ftsN"
                     /locus_tag="WGR04_001146"
     CDS             complement(3546..4544)
                     /gene="ftsN"
                     /locus_tag="WGR04_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0794706.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            complement(4557..5831)
                     /gene="folC"
                     /locus_tag="WGR04_001147"
     CDS             complement(4557..5831)
                     /gene="folC"
                     /locus_tag="WGR04_001147"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ0794707.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DFDHQAFLGDTVEQVGFEKAGVFRGGKPAICGQNPAPASLLSHAEAIGAKLLMVQRDF
                     EFHALENIQWNYRFRPQHSDSPARNRNSLPFPALRGAYQLSNAACALTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            complement(5863..6309)
                     /locus_tag="WGR04_001148"
     CDS             complement(5863..6309)
                     /locus_tag="WGR04_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ0794708.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKEKTAKAAALSGSSMVTPYALF
                     LQWDTGGKTVRHCITHDMADPESYRRLKVWVLWRQPKKTAETDTPS"
     gene            complement(6314..6673)
                     /locus_tag="WGR04_001149"
     CDS             complement(6314..6673)
                     /locus_tag="WGR04_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794709.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            complement(6746..7474)
                     /locus_tag="WGR04_001150"
     CDS             complement(6746..7474)
                     /locus_tag="WGR04_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0794710.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            complement(7647..8426)
                     /gene="rsmA"
                     /locus_tag="WGR04_001151"
     CDS             complement(7647..8426)
                     /gene="rsmA"
                     /locus_tag="WGR04_001151"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ0794711.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            8623..9312
                     /locus_tag="WGR04_001152"
     CDS             8623..9312
                     /locus_tag="WGR04_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ0794712.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLHERGIASCPTVLKAAREHGNASDKFLKVVETVDLAAVLAKIPDCRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
     gene            9497..10699
                     /gene="trpB"
                     /locus_tag="WGR04_001153"
     CDS             9497..10699
                     /gene="trpB"
                     /locus_tag="WGR04_001153"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ0794713.1"
                     /translation="MKNYHAPDEKGFFGEHGGLFVSETLIPALQELADAYKAAKNDPE
                     FWEAFRHDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(10878..16313)
                     /gene="iga"
                     /locus_tag="WGR04_001154"
     CDS             complement(10878..16313)
                     /gene="iga"
                     /locus_tag="WGR04_001154"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ0794714.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADEIKQRDNAGTIKGYGEHHWKTTGT
                     NSHIGSTAVRLAGNERGANNGQNVTFENNGTLVLDQNINQGAGGLFFKGDYTVKGINN
                     DITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVIL
                     NQKADSNQKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFEH
                     IRNVDEGARIVNHNTGHTSTITLTGKSLITNPNSLSVHSIQNDYDEDDYSYYYRPRRP
                     IPQGKDLYYKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQEEAKKNAMNHKNN
                     QRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLSG
                     RPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKATEIAVNQSASFSSGRNVS
                     DITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALNSFGATQINGNV
                     NLSQNAALTLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNNA
                     SDAQSANKYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGHYQLHVQDKTGEP
                     NQEGLNLFDASSVRDRSHLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRVK
                     PAPSPATNTASQAQTDSAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKRQQAEA
                     ERKSAELAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREAAELSAKQKVEAE
                     REAQALAVRRKAEAEGAKRQAAELARRHEKEREAAELSAKQRVGEEERRQTAQSQPQR
                     RKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELARRQQEERKAAELLAKQR
                     AEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELSANQKAAAEAQALAARQ
                     QKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAAKRQQEARQTAELARRQ
                     EAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRKRRAAPQDYMAAS
                     QNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDALEDENVSES
                     VDTSDKQPQDNTELHEKYENDYEEIPLDALEDEDVSESVDTSDKQPQDNTELHEKVET
                     VSLQPRAAQPRAQAAAQPQAQAVAQADAVSTNTNSALSDAMASTQSILLDTGASLTRH
                     IAQKSRADAEKNSVWMSNIGYGRDYASAQYRRFSSKRTQTQIGIDRSLSENMQIGGVL
                     TYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRSRLQTQQKANFNR
                     ASIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKVSSMSVKTLTAGLD
                     FAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFAYKADNQQQYSAGAALLYRNVT
                     LNVNGSITKGKQLEKQKSGQIKIQIRF"
BASE COUNT         3407 a         4134 c         4575 g         4693 t
ORIGIN      
        1 tcattccggc ggcaattttt gttgctttaa cgggataggc ggttggtggt tgcgataaag
       61 gcggcgactt tggctgggtc ttttttgcct ttagacgctt ccacgccgcc ggatacatcg
      121 accgcttccg ctccggtgat gcggacggct tcgccgacgt tttcaggggt cagcccgccg
      181 gcaagcaccc acggtttgcc cgaatattcc gccagcagcg tccagtcgaa gcggtgtccg
      241 gtgccgccgt attccgaagg gtggtaggca tcgaacagca gtgcctgagc gtcggggaag
      301 cggtcggcgg cgtttcggat gtctgatgcc gtctgaacac gaatggcctt gatatagggg
      361 cggtcgaact ggcggcagaa tgtgtcgtct tcgtcgccgt ggaattggat gatgtgtatc
      421 ggcacttcgg caaggatgcg gcggatgttt tgcgcgcttt cgttgacgaa aagggcgaca
      481 acgctgacaa acggcggcag tgcggcggtg atttttttgg cgcgggcaat atcgacggcc
      541 cggctgctgc cttggaaaaa gaccagcccg acggcatctg cacctgccgc tacggcggcc
      601 gatgcgtctt ccggtgtggt gatgccgcag attttggtgc ggattttcct cattcggtat
      661 tcctttattt gggaaacggc gcacttgcac cgtttcagac ggcattcccg atcagtcgat
      721 tttgatgtat tcgacagaaa ggatttcaat ttcctcacgc ccttccggcg tgttcaaaac
      781 cacttcgtcg ccttcgcgcg ctttaatcag gcaacgcgcc aacggcgaaa tccaagaaat
      841 tttgttttgc gcggtatcga tttcatcgat gccgacgatt ttgacggttt gctcgcgccc
      901 gtcgccgcgc aacaggccga cggtcgcgcc gaaaaatact tggtcggtcg cttcgcgcaa
      961 ttcgggatcg acgacgacgg cggactccaa acgcttggtc aggaaacgga tgcggcggtc
     1021 gatttcgcgc atacggcgtt tgccgtaaag atagtcgccg ttttcgctgc ggtcgccgtt
     1081 gcctgccgcc cagttgacga tttggacgat ttcggggcgt tctttgttga ccagttgata
     1141 aagctcgtct ttcaatgcct gccatccggc gggcgtaatg tagtttttgg tttcggtact
     1201 catattgtgt gcggatgaaa cgggaaatgt gatgccgata tgggaaatgc cgtctgaaaa
     1261 cccggcgttc ggatttcaga cggcatcgcg gtttgggaag ccttattctt cgtcgcccgc
     1321 atcgctgatg ctgatgctgt gttccatcct gctcgggtgg attttcagac cgccgcagcc
     1381 ggatttctcg gcagacaggc ggtcgaggta ggcatcatcg atgtcgccgg tctgataaat
     1441 gccgttgaaa caggacgaat cgaaggattc gattttcgga ttgagtgctt tgacgacggc
     1501 ttccaaatcg cccaagtctt gaaatacgat gccgtccgcg ccgatttcgg cggcgatttc
     1561 cgccgcgctg cgcccgttgg caatcaactc ttcgcgcgtg ggcatatcaa tgccgtacac
     1621 attgggatag cgcacttcgg gcgcggcgga ggcgatatag actttgcgcg cgcccgccgc
     1681 gcgtaccatt tcgacgattt cgcggctggt cgtcccgcgc acgatggagt cgtccaccag
     1741 caacacgctt ttgcctgcaa attcggtttc catcgggctg agtttttggc gcacggattt
     1801 tttgcgcgtc gcctgaccgg gcatgataaa ggtgcggccg atatagcggt ttttaatcaa
     1861 gccctcgcgg tagggtttgt cgaggtggac ggcaagctcc atcgcgctgg ggcggctggt
     1921 gtcgggaatg ggcatcacga catcgatgtc gtccacgggc agctcgcgtt tgattttttc
     1981 cgccaaggac acgcccatat ccaagcgcga ttggtaaacc gatacgccgt cgattacgga
     2041 gtcggggcgg gcaaaataaa catattcaaa aaggcagggg ctgagtttgg cacggtcgct
     2101 gcattggcgg gcaatcattg tgccgtcaaa gccgacaaat accgcttcgc ccggccggat
     2161 gtcgcgttcc aaatcgtagg caagcgcgtt gaaggcgacg gattcggagg cgacggcata
     2221 ggatttcctg ccttcgttgt cggtttgcga acccaatacc agcgggcgga tgccgtaagg
     2281 gtcgcggaag gcaagcatac cgtagcccgc aatcatggca accacgccgt atgcgccgcg
     2341 caccaggcgg tggacttcgg caacggcgtt gaaaatattg tcggcattga gccggtgcgg
     2401 gtcggcgttt ttagagactc cgcggcgtaa ttcgtgcgcg aatacgttga gcaaaacttc
     2461 ggaatcggag ctggtgttga cgtggcgcag gtgtttgttg cacacgtttt cataaagttc
     2521 ggccgtgttg gtgaggttgc cgttgtgcgc caaaacgatg ccgaaaggcg agctgacgta
     2581 gaaaggctgc gcctccgcgc tgctgccggc gttgcccgcc gtgggataac ggacgtgggc
     2641 gatgccggcg ttgccgacca aatcgcgcat attgcgcgtg cggaacactt cgcgcaccat
     2701 ccctttgcct ttgtgcatat ggaaggtgcc gccttccgcc gttgcaatgc ccgccgcatc
     2761 ctgccccctg tgctgcaaca tctgcaaacc gtcgtacaga agctggttca cgggttcatg
     2821 actgaccaaa cctaatacgc cgcacatatc gtcttctccg attcgaggtt taagggtaaa
     2881 acggaattat aaagcaaacg gtggtttttt gcctgaattg ttgacaatat ttgagtgaag
     2941 gacagatagg tgggtttatg gaggataaga tttaatctgt gatatggctg aggaaaggaa
     3001 aaaaacattt cagacggcat aaaagaggat gccgtctgaa atatccgtat ggcaatcaat
     3061 cgtcttccaa agctccatct ctgtcgtccg catgctttaa cactgcttcg gaaagcgaca
     3121 cgaaaaacgg tatggtatag gattgccgcc attcttcggt atcgggcagg tcggtttttg
     3181 aagcgagcat gaccagcagg gtaataatca aaatgccttt caatgcaccg aatacgccgc
     3241 ccaaaatgcg gttggcaaag cccaaaccga ccgccgaaac tgccccggtc agcagcgagc
     3301 ggagtatttt ctggatcaga caggcaatga cgaataggga aatgaacgac agagccaatg
     3361 caaacaggcg gggttggaac gaggcaaagg cgaggtcggc gaagggtgcg gcaaagagtt
     3421 tggcaaaaaa gaaggaaacc acccatgccg ccatcgaacc tgcctccgca atcacgccgc
     3481 gcatcgtgga aatgacgatg caggcggcga tgatggcgaa gacgaggagg tcggcaacgg
     3541 ggaggttatt cattcgttac ctgaccggcg ataccgtgta cacgcaattt gttcaaatcg
     3601 cgttcggcat cccttgcgtt tttatagttg cttgatttga cgcggtaaac tttgccgttg
     3661 tcggtcataa tttcggtgat ggtcgaatcg atacccgccg ccttcatttt gcgctggagg
     3721 cttaaggcgc gttctttttc ggcataacct gcctgaatgg cggctttttt accggatttt
     3781 tccttctcgg cggttttggt cttgtccgct ttgtcggttt tttgtgccgt ttcgtgtttt
     3841 ttgccgtccg aacggtcttt ttcggctgtt tttttgcttt cagccttgtc ggcttttttc
     3901 gcttctttta ccgcgctgtc ggattttgcc gtatccggtt tggttttttc ggcggcagtt
     3961 ttcggtttgt cggcaacttt ttcggcggtt ttggtttctt tggctttggg cttggctttg
     4021 gcagtgcgtt ccgctttttg cggttttgtt tcggcagtgc gtttcggttt ttcaaccgct
     4081 accgtatccg tactgtcggc agttgccggc actttttcgg cagcgcgttg ttttgcctgc
     4141 ttcggtgcgg ttttggcggt ttctgcctgt tgcagtttct cggatgcttc caaacctttg
     4201 atgttgctgt cttcgaggcg gtcgttaatc agcaccagcg gcgcgcctac gttttcaggc
     4261 tcgctgattt cgctgtcggc ggcagaaggc ttgtcttcgc ctgccaagtc ctgcggtttg
     4321 tcggcggcgg atttcaaggc aggggtttgt gccgcacctg ccgctttgtt ttctacgccg
     4381 cttgtttcgc cggcagtctg ttcggcaggg ccggaactga gggcggctgc cagcaggatg
     4441 caggaggcgg caaccaggca acttgccgtt acgaggcggc ggcggttgcg ccgtttgagt
     4501 tgttcgtaac cgctcaggac ttcgttttgt ttgttttcgg acatagaagt ttccttttaa
     4561 agtaccgaca tgacatcggc aacggtatga aatgagccga aaacgacgat tctgtcgttc
     4621 tcgcccgctt ttgaggctgc cgcccggtaa gcttcgcgga cggcggcgaa tgtctgtatg
     4681 ttttcgatgc tgtgttcgtg cagtttgttt tgcaggctct cgagcgtcat gccgcgcggt
     4741 acgtccaacg gtgcgatata ccactcgtca aactggtctt taacgatttc caacacgccg
     4801 tctatgtctt tgtcggacag catactgaat acggcggtgc gtttttgcgc aaacgccaaa
     4861 ttaatcaggc tgcggcgcag ggcgcgggca gcgtggggat tgtgtccgac gtccaaaacg
     4921 gtcagcggcc ggccgggcag gacttggaag cgtccgggat tttcaaccag cagcaatccg
     4981 cgtttgattg caccgatgtc caccggcaat ttgtcgttca agcattccaa cacggtcagc
     5041 gcgcaggcgg cgttggaaag ttggtatgcg ccgcgcaatg cggggaaggg cagggaatta
     5101 cggttgcgag cggggctgtc tgaatgctgc ggtcggaagc ggtagttcca ttggatgttt
     5161 tccagcgcgt gaaactcgaa atcgcgctgc accatcagca gtttcgcgcc tatggcttcg
     5221 gcgtgtgaaa gcaacgatgc gggcgcggga ttttgaccgc agattgcggg tttgccgccg
     5281 cggaacacgc ctgctttttc aaagccgacc tgctcgaccg tatcgcccaa aaacgcctga
     5341 tggtcaaaat ctacactagt aaccaccgcg caatcgccgt caaatgcgtt gaccgcgtcc
     5401 aagcgtccgc ccaagccgac ttccaatatc atcacgtcaa cctgttcgcg catgaagatg
     5461 tcgacggccg ccaacgtatt gaattcaaaa taagtcagcg atatttcgcc gcgcgccgct
     5521 tcgatgcgct cgaaagaggc aataatcgta tcgtccgaaa cgggttcggc gttgatggcg
     5581 atgcgttcgt tgtaacgcaa taaatggggg ctggttaatg tgccggtttt gtagcccgcc
     5641 tgtttgtaaa tctgtgtcag gtaggcgcag accgaacctt tgccgttagt tcccgcgaca
     5701 acgacgacgg ggcattgcgg cgtgagcttc atccgttttt tcacttcgcc tacgcgttcc
     5761 aagcccatat cgatcaaacc gccgctgtgg gcggtttcca aatgggagag ccagtcttgc
     5821 agtgttttca ttgagtgttc cgtgttcaaa tgccgtctga aatcagcttg gtgtatcggt
     5881 ttcggcggtt tttttcggct gccgccaaag cacccagact ttcagccgcc ggtaggattc
     5941 cgggtccgcc atatcgtggg tgatgcagtg gcggacggtt ttgccgccgg tgtcccattg
     6001 taagaataag gcataaggcg taaccatact gctgcccgac agtgccgccg cctttgctgt
     6061 tttttctttg ccggatacga tttccgcctg tccgtcgcgg tctatggtaa tggcggttat
     6121 ggcatggcgg tgtttcagat tcgttatcct gagcgagtat gcgtaacttg ccgccaaagc
     6181 cgccaaaccg aaccacatca tccggccgta aaaccaggtc aggcagacgg caagggaagc
     6241 cgagtggagc gatacggtca ggatgttcag gatgcgggac ggcctcaatg ctgtctgaaa
     6301 ggcgcgcacg gcgttacatc atattgtcga acacgggcgt gatgttcaaa tccgcttctt
     6361 ccatatccaa caccatatcg tggatttcga tgtcgaaaaa ttcccaaaac gccttcagcc
     6421 ccatatcttg cggccattta tccttgtcga tgtcccagcc ggcgagttcg gcttcaaaaa
     6481 tctggcggta gcgttcgtcg aaataggaaa cgacggcttc cggttcgtcg aactgcggaa
     6541 cgaggaagac ggagcagttg gcacgaagct gctctatggt caggtcgggc atattctcgt
     6601 cggtgctttt gagccattcc aaaaagcgga cggtcggctt gaggacgacg gcggtgcggt
     6661 caacaaaata catggttttc tttctcaatc atcttgcggt gtcgggctat gccgtctgaa
     6721 cgttcggttt tcagacggta tagcatcagt gggtcatgac ctgttgcagg aactgcttgg
     6781 cgcgttcgtg tttcgggttg gtgaaaaacg cttcgggcgt ttcgtcttcg aggatttgcc
     6841 ctttatcgac gaaaatcacg cggtcggcaa cttcgcgggc aaaccccatt tcgtgggtta
     6901 cgcacatcat ggtcatgccg ctttctgcca agtctttcat cactttcaac acttcgccga
     6961 ccatttcggg gtcgagtgcg gaggtcggtt cgtcaaacaa cattacgcgc ggttccatcg
     7021 ccaaaccgcg tgcaatcgcc acgcgctgct gctggccgcc ggaaagttgg gacggaaaag
     7081 cgtctttttt gtgcgccagt ccgacgcgtt ccaaaagttc catcgctttt ttctccgcct
     7141 gttccgcatt ttgaccttta accttcatcg gtgcgagggt gatgttttcc aatacggtca
     7201 ggtgggggta gaggttgaaa ctttgaaaca caaagccgac ttcttcgcgg attttgttca
     7261 aatcggtttt tgggtcggca acgttgacac cgtccaccca aatctcgccg ctttcgatgc
     7321 tttcaagctg gttgacggtg cggatgagtg tggatttgcc gctgcccgaa ggtccgcaga
     7381 cgacgaccac ttcgcctttt ttgatttcca agttcacgcc gttgatgacg tgcaggtctt
     7441 tgaaatgttt gtgtacgttt ttgaatttga tcatgtcttt tctttcagac ggcatttgcc
     7501 gttgcaggtt gtcgttacgg gagctccata tgatgaagcg tgtagcgtct gacgtcaaaa
     7561 aaacggtcgt tcggattggt caggcaggct gcaagcggca gtatcagggg taaaagcagg
     7621 tatttcgtca tcggcttact cccttttcag acggccttgc ccgccagata attgctcaac
     7681 gccacatatt tctccggcgc gatatgttcg gcgcggtctt gcggattgat gccgactgcc
     7741 tgcaaatcat cgtcgcctgc aagctctttc agattgttgc gtatggtttt gcggcgttgg
     7801 tggaaggcga gtttcacgag tttggcgaaa tgctcgaaat cgtccgcctt gccgatacgg
     7861 tgtttcaccg gaatcatgcg gacgacggcc gaatccactt tcggcgcggg gtcgaacgat
     7921 tcgggcggca cgtcaatcag catttccata tcgaagaaat attgcagcat cacgcccaag
     7981 cggccgtagt cgttgctttt cggcgcggca accatacgat cgaccacttc tttttgcagc
     8041 ataaagtgca tatcgacgac atcgtccgcc acctccgcca gcttgaacaa aagcggcgtg
     8101 gaaatgttgt agggcaggtt gccgacgatt ttctttttgc ctgcgatgcc gttgaaatca
     8161 aactgcaata catcgccttc gtgaatcacc agtttatccg caaacggcag cgttttcaga
     8221 cggcatacga tgtcgcggtc gatttcgaca acgtgcaggc ggttcagctt tttcgccaaa
     8281 ggttcggtaa tcgccgccaa acccgggccg atttcaatca cgacatcatc cgcctgcggg
     8341 cgcacggcgt tgacaatatc gctgatgatc cgcgtgtcct gcaaaaaatt ctgcccgaaa
     8401 cgcttgcggg ctttgtgttc tttcatcgtg tttccttttc ggttgaaacc ccgcccttta
     8461 gggcggtaga atcagactct atttgggagg ggcgtaactc cttccaaatc aggatggcac
     8521 atagggcggt gctttaagtg tcgtcctgtg tgttggaaca taaatgtgtt tacagtatcc
     8581 gtttgatgtc ggcattgtaa ccgaaaacgg cagggcgtga taatgctgtt tgaaggcttg
     8641 ccgtgtttgg cggtttggtg caaaaaccgg ctgtctgccg ttttgcctgt tggaggattg
     8701 aacgtgtctg aaaatctgct tgaaatcgaa acccatccct tcgattccgt gttgccgccg
     8761 aaggctgcgg tcatgatgat ggggacgttt ccgcccaagg aagacaaacg cgcgatgcag
     8821 tttcattatc cgaatttcca aaacgatatg tggcgcgttt acgggctggt gttttttaat
     8881 gatgcggcgc atttccaaag gttgtctgaa aaagcgtttg atgccgagaa aatcaaggcg
     8941 tttttgcacg aacgggggat tgcgtcctgt ccgaccgtgt tgaaggcggc aagggaacac
     9001 ggcaatgcgt ccgacaagtt tttaaaggta gtcgaaaccg tcgatttggc ggcggtgttg
     9061 gcgaaaatac cagactgccg ccatatttgt acgacaggcg gcaaggcgac ggaaatcctg
     9121 ctcgatattc agggtggcgg catcaaaatg ccgaaaacgg gcgaaaccgt gccgtttccg
     9181 tttgccggac gggatttgac gctgacgcgc ctgccttcga cttcgcgtgc ctatcctttg
     9241 agtttggcga aaaaagcggc ggcgtatcgg gcgttttttg aaatggcggg cttgtgtgaa
     9301 aaacagttat aattgccgac aatttcccgt tcggacggca tgtttgaaaa atatagtgga
     9361 ttaacaaaaa tcaggacaag gcggcgaagc cgcagacagt acaaatagta cggcaaggcg
     9421 gggcaacgcc gtaccggttt ttgttaatcc actataaatt gccgtctgaa aatttgaagc
     9481 acaaggacga aatctgatga agaactacca cgcgcccgac gagaagggct ttttcggcga
     9541 acacggcggg ctgttcgtgt ccgagaccct gattccggct ttgcaagagc tggcggatgc
     9601 ctataaggca gcgaaaaacg atcctgaatt ttgggaagcg ttccgccatg atttgaaaca
     9661 ttatgtcggc aggcccagcc ccgtttacca cgccgcgcgg ttgtccgaac atctgggcgg
     9721 cgcgcaaatc tggttgaagc gcgaagactt gaaccacacc ggcgcgcaca aagtcaacaa
     9781 caccatcggt caggcactgt tggcaaaacg catgggtaaa aaacgcgtca tcgccgaaac
     9841 cggcgcgggt cagcacggcg tggcgagtgc caccgttgcc gcacgcttcg gtatgacttg
     9901 cgacgtgtat atgggcgcgg acgacatcca acgccaaatg cccaacgtgt tccgtatgaa
     9961 attattgggt gcgaacgtgg tcggtgtaga aagcggcagc cgcacgctga aagacgcgat
    10021 gaacgaagcc atgcgcgaat gggtcgcccg cgtggacgac acgttctaca tcatcggtac
    10081 cgccgccggc cccgcgccgt atcccgaaat ggtgcgcgat ttccaatgcg tgattggcaa
    10141 cgaagctaaa gcgcagatgc aggaagccat cggcagacag cccgacgttg ccgttgcctg
    10201 cgtgggcggc ggatcgaacg ccatcggttt gttccacccg tatatcggcg aagaaaacgt
    10261 gcgcctcgtc ggcgtggagg ctggcggttt gggcgtgaac acccccgatc acgccgcgcc
    10321 gattacttcg ggcgcaccga ttggcgtatt gcacggtttc cgcagctatc tgatgcagga
    10381 cgaaaacggt caggttttgg gtacgcactc tgtttccgca ggcttggatt accccggcat
    10441 cggcccggaa cacagccatc tgcacgacat caagcgcgtc gaatacactg ttgccaaaga
    10501 cgacgaagca ctcgaagcct ttgacttgct ctgccgcttc gagggcatca tccccgcgct
    10561 cgaatccagc cacgccgttg cttgggcggt gaaaaacgcg ccgaaaatgg gcaaagacca
    10621 agtgattctg gtcaacctat ccggtcgtgg cgacaaagac atcaataccg tcgcgaagct
    10681 caaagggatt aaactgtaac ctcgtccgtc tgatatagtg gattaacaaa aaccagtacg
    10741 gcgttgcctc gccttgccgt actatctgta ctgtctgcgg cttcgtcgcc ttgtcctgat
    10801 ttttgttaat ccactataaa aatgccgtct gaagcctgag ttcagacggc attttatttt
    10861 gctatgaatt tagtatttta gaaacgaatc tgtattttaa tttgtccgga tttttgtttt
    10921 tccaattgtt ttccttttgt aatactgcca tttacgttta atgtaacatt acggtacagt
    10981 aacgcggcac ctgctgaata ttgctgttga ttatctgctt tataggcgaa ggaattaccg
    11041 cccacattca cgccaccttt gccataattg gcaaagtaag ctgcagataa caagggtttt
    11101 acggtaaggt tgccgacttt aaaccgataa gcaaaatcca gtccggccgt cagtgttttc
    11161 actgacatag aacttacttt aacactgtcg ttacccaact tgtaatctgc agatgacagg
    11221 cggctgtaac ggatccccgc actggggaca atctcgaatt gattgatttt cagcgtattg
    11281 cccaaagtaa ggccggtttg gatgcttgct cggttaaagt ttgctttttg ctgcgtttgt
    11341 aaccggcttc tcaagctgcc tgcaccaata tcgccggcca cataccaagc atcatttaaa
    11401 taatacttac cataaaggtt ggcttgcaca aaagtatttt tgccgctcgc ctgatcaaaa
    11461 gtatgctgac tgtcagagta agtcaatacg ccgcctatct gcatattttc ggacaagctg
    11521 cggtcaatgc cgatttgtgt ttgcgtgcgt ttcgaactaa accggcgata ttgtgcggaa
    11581 gcataatcac ggccataacc gatgttcgac atccaaacgc tgtttttttc ggcatcagca
    11641 cgtgattttt gtgcaatgtg ccgtgttaat gaagcacctg tatccaacaa gatagattgc
    11701 gtgcttgcca ttgcgtcaga taaagccgag ttggtattgg tgctgactgc atcggcttgc
    11761 gcgacggctt gggcttgcgg ctgcgcggcg gcttgggctc gcggctgagc ggctcttggt
    11821 tgcaaactca ctgtctcaac tttttcatga agttccgtat tgtcttgagg ctgtttgtct
    11881 gatgtgtcaa ccgattcgga tacatcttca tcttccaacg catccaaggg gatttcttca
    11941 taatcatttt catatttttc atgaagttcc gtattgtctt gaggctgttt gtctgatgtg
    12001 tcaactgatt cagatacatt ttcatcttcc aacgcatcca aggggatttc ttcatagtca
    12061 ttttcatacc agtccggatt atgcaaggcc ctgggtgctg cgtaagctga cgaatcaaat
    12121 gatggcgagg gcggtgccgg cagagtagat ctgcgtccgc gacgtttcgg acgattttgg
    12181 gaagctgcca tataatcctg aggtgcggca cggcgttttc ggcgttgcgg ctgggattgg
    12241 gctgcttgac ggtgctcttc ttcctcagct tttcgtttcg ccgattctgc cgcttctcga
    12301 tctgtttcgg ctttttgttt tgccgacaac tctgctgctt ggcgttccgc ttcttgacga
    12361 cgtgcaagtt cggcggtctg gcgagcttct tgctggcgtt ttgctgcttc ggctgctgcc
    12421 ctttgcttgg caagttcggc ggttttgcgt tcggtttccg ctttttgttt gacggctaac
    12481 tctgcagctt ttcgtgcttc ttcctgttga cgcgcaaggg ctttttgttg gcgtgccgcc
    12541 aatgcttggg cttcagctgc ggctttttgg tttgccgaca attctgccgc tttgcgttct
    12601 gcttcctggc gatgtgcaag ttcggcagct tggcgttttg cctcttcggc ttctgctttt
    12661 cggcgcgcag ccaatgcttg agcctcacgt tcggcttctg ccctttgttt tgccaacaac
    12721 tctgccgctt tgcgttcttc ctgctgtcgg cgcgcaagtt ctgcttgggc ttgggcaatt
    12781 tccacattgt tttgctgtac cgaacggtgt ccgcggcgtt taggacggtc ttgggaagct
    12841 gccatataat cctgcggtgc ggcacggcgt ttgcggcgtt gcggctggga ttgggctgtt
    12901 tgacggcgtt cctcttcccc gaccctttgt tttgccgaca actctgccgc ttcgcgttct
    12961 ttttcatgcc ggcgtgcaag ctctgcagct tggcgttttg ccccttcggc ttctgctttt
    13021 cggcgtaccg ccaatgcttg agcctcacgt tcggcttcga ctttttgttt tgccgacaac
    13081 tctgccgctt cgcgttcttt ttcatgtcga cgtgcaagtt cggcgctgct gcgttcttgt
    13141 tctgcttttt ggcgggtcgc cagctctctt gcttcgcgtt cggcttctgc tttttgtttt
    13201 gctaactctg ccgatttacg ctctgcttct gcttgctgac gcttcacttg ctccgctttt
    13261 gcttgttggc gttttgcttc ttcggcttga tttgcctgcg ggctaggcgg tgcgacgacg
    13321 atattttgag gcttggcaat ttgtgcactg tccgtttgtg cttgagaagc cgtgtttgtg
    13381 gcaggagacg gggccggttt gactcggcgg cggttctcgg cataaggatt gtacaatcgg
    13441 gtaatgccat tttctgtttt gattgtataa cgcaatgcgc ctaaatctac atggttattc
    13501 gccaaggaaa cggaaaggtg cgatcggtct cgtacggatg atgcatcaaa gagattcagc
    13561 ccttcctgat tgggttcgcc tgttttatct tgaacatgga gctgataatg accggatgcg
    13621 gattccttca caagcacttt atccccaaga ttttttgcca agtgcgtcag ataatgaaaa
    13681 tgcccgttac cggataaatg attgattttg agcgtgtggt atttatttgc actttgcgcg
    13741 tcggaagcgt tgttcaaatg aatatggcta tccgccaatg acagattgtg tacttggctg
    13801 tcgccggtca aatgccattt gctatgttga tttaggctga cacggctgtt tccttgccct
    13861 tgaatttgcc cccataatgc agccttgccc aaagtcaatg ccgcattctg actcaaattc
    13921 acattgccgt taatctgtgt cgctccaaag ctgtttaaag ccttatcgga taagttgcct
    13981 gtgttgcagg taacataacc ggtatagtcc gagcgcacgc aaacctcatc gccgtttttg
    14041 taacccaaat ttactttggc gttgtctgtt gcggtgatgt tggcggtgat gtcggataca
    14101 tttctgccgg aagagaatga tgcggattga ttaaccgcaa tttctgtggc tttgaatgtg
    14161 cggtttatcc agtcgtcttc aaatacgact tcattgtttt tggagaaatg tgcgtctttt
    14221 cgggctgaag atttgttcac aaaatctctt gcgtgtggtg ttggacgacc tgataacaag
    14281 acattgcctt gagttacgct tatttttccg tttaaattag tgccacctgt taacaagaaa
    14341 cggttttgcg cgcttttgcc gttgaaatta aggtttaatg caccgttatg tccttttccg
    14401 ttttcttcgc caaagaaacc gctaaaaccg ctaatacgct gattgttttt gtggttcatc
    14461 gcgtttttct tggcttcttc ttgtgtgctg cccattaaaa tccagtcgtt attttccgtt
    14521 tgtccgtttt cgggcatcgg tgcgttcacg ctgccgccgg attttagggc gtaataacga
    14581 tagtttttgt aataaagatc tttgccttgt gggatgggtc gccttggtcg gtaataataa
    14641 ctataatcat cttcatcata gtcattttgt atactatgta ccgaaaggct gttcggattg
    14701 gtaattaaag atttacccgt tagcgtgatt gtggaggtgt ggcctgtgtt gtggttgaca
    14761 atgcgcgcgc cttcatccac gttgcggatg tgttcaaaag tcaagtcatt gccattggca
    14821 tccaaacgac cgccacggaa accgaaatat aggttatcgg gattaatctg atttgaacta
    14881 tttaatacca atgtaccgcg tccgctgaca atgccgactt gggagaaagc ctggactttt
    14941 tggtttgaat cggctttttg attcagaata accgtaccgt cgccgacttt taattgccct
    15001 tggttaacgc ctgtgccgtt tatttctaat gtgcctttgc cgattttcgc caatctgtcg
    15061 ccattcggat ttttgacttg ccaaacgact tttttgccgt cggcaacatc aatccccgca
    15121 cctagccaag tgatgtcatt atttatacct ttgactgtgt aatcgccttt gaaaaacaga
    15181 ccgcccgcgc cttggttgat gttttgatcc aataccaaag tgccgttgtt ttcaaaggta
    15241 acattctgtc cgttgtttgc acccctttca ttgccggcaa gccttaccgc tgtcgaaccg
    15301 atatggctgt ttgtgcccgt ggttttccaa tgatgttcgc cataaccttt gatggtgccg
    15361 gcgttatcgc gttgtttgat ttcatctgca aattcttttt tatagatatt ccattcttgc
    15421 caagagtttt tttgatagcc tgcccaataa tcgtaagcac ctaaaaagac ccagcgtttt
    15481 tcttgtttat cataagcaaa taatggtgaa ccgctatcgc ctaacacagc atagttagtc
    15541 aatgcgtttt gagataaaac ttctttgagt ttttctgggg aatgatgttt tgaattatca
    15601 ccgaagccaa tcaagccttc ttgatttaga ttcgatgtga cattcacgtc ttgataaggc
    15661 gtacctgcaa tggcataacg ataagctcgt gaaagctctg tcatattgta gcggctgtta
    15721 tattcaaatt gcgtacctgc tccaactcgc acaaactcag aaaaacggtt tttatcttta
    15781 taggtttcaa cgccgccgcc tgcactggtt ggtgcaatag gtgcgacttc tgttacgaat
    15841 ttattaaggc gtgccatgtt gtagtcttca agacgacctt gattaccgtg atgccaattt
    15901 ttatttggtt cgtagtcatt ttgtgcaact gaacgatatt cgttttcatc attgctaaca
    15961 tctaagtgcc cattgtgatg cccgtaataa gagatttcgt ctccttttac atgtttgacg
    16021 ctgacggcat actggggatc gatgacggtt aatgtacgtc tgttgacatc tgcaacgcta
    16081 aaatcaatca tcggtacgtt ggataatgcg ttgccgatgt tttgaccttg tttgtttttc
    16141 actgataaat cggttgcgcc gacaaaaaat ttgcctttgt tttctgcaaa gtcacggaat
    16201 atttgataat cgacatcgtc tctgaccaat gccgcttctg agtatggcgt aagggcatag
    16261 gcaagaaaga tggataagga tatggcgtta attttaaaac gtttggtttt cataaggttt
    16321 taccgtttta agggtgataa tgtcttgtat tttacgccgg tttggcggtg ttgactattt
    16381 tttgttgtgt aaatcattaa ttttggtgtt ttattgtcaa tttaaaaaaa gaatcccgat
    16441 gtttttattt ccgcttcctt tgttctgtta ttcaagcgaa ggcgggaagc cgattttcgg
    16501 ggttcggttc ttccgttaaa tttctgcgac tttttgtttt tggattcccg cttgtgcggg
    16561 aatgacggga tgtaggtttt cttaaccctg cgtcctagat tcccgctttc gtggtaatga
    16621 cgagatgggg gttcgtggga atgacgcggt gcaggtttcc gtacggatgg attcgtcatt
    16681 cccgcgcagg cgggaatcta gaccttagaa caacagcaat attcaaagat tatctgaaag
    16741 tccgagattc tggattcccg ctttcgcggg aatgacggga ttttaggttt ctgattttgg
    16801 ttttctgtt
//
LOCUS       DAWFJR010000039        16189 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWFJR010000039 DAWFJR010000000
VERSION     DAWFJR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16189
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.039"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(201..2990)
                     /gene="ileS"
                     /locus_tag="WGR04_001155"
     CDS             complement(201..2990)
                     /gene="ileS"
                     /locus_tag="WGR04_001155"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ0794715.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQVARQKKDFIRLGVLGDWDRPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVGYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGDHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYIGETPRVAGMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFIGMDKAGNDGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQKIEEKGIE
                     AWFSLDKSELLSAEDCENYDKLSDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYAVVLARRLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMASYL
                     NALGEELRFALLVSKAEVKVGDELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
     gene            complement(3132..4055)
                     /gene="ribF"
                     /locus_tag="WGR04_001156"
     CDS             complement(3132..4055)
                     /gene="ribF"
                     /locus_tag="WGR04_001156"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ0794716.1"
                     /translation="MRIRLGRHNSPDFPHGAAVTIGNFDGVHLGHKHILQKLRFEADT
                     RGLPVVAVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVVDSGCSQKLEVHLFDFQGDLYGQRLNVR
                     FLHKLRDEEKFDGMEELKRQIAADMEAAKCW"
     gene            complement(4354..5649)
                     /gene="tyrS"
                     /locus_tag="WGR04_001157"
     CDS             complement(4354..5649)
                     /gene="tyrS"
                     /locus_tag="WGR04_001157"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ0794717.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     SQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLYQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEISDGINAVEALVKTGLAASNKEARGFVNAKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(5712..7580)
                     /locus_tag="WGR04_001158"
     CDS             complement(5712..7580)
                     /locus_tag="WGR04_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0794718.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKDWLPGGFLGVDIFFV
                     ISGFLMTTILHREMSGGGRFSLKAFYIRRIKRILPAFFAVLAATLAGGFFLFTKDDFF
                     LLWKSALTALGFASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVA
                     RKSLRVQFGFLAALCALSLAASFMPSAFDKYYLPHLRACEMLVGSLTAVWMRCRQPAV
                     GRRCAAAGALFAVCILSTCLFSYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLKKF
                     FQWKITVAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSTAAAIVLILLLSLISYHCI
                     EKPFKKWKGSFAQSVLWIYALPMLILGAGSFFAMRLPFMAQYDRLGLTRSNTSCHNNT
                     GKQCLWGDTEKQPELLVLGDSHADHYKTFFDAVGKKEKWSATMVSADACAYVEGYASR
                     VFQNWAACRAVYRYAEEHLPLYSKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRMLHK
                     LSSEKQAVYLMADNLASSYNVQRAYILSSRIPGYRQTLRPDDESTLKANARIRELAAK
                     YPNVYIIDAAAYIPANFQIGGLPVYSDKDHINPYGGTELAKRFSEKQRFLDTRHNH"
     gene            complement(7850..8941)
                     /gene="ychF"
                     /locus_tag="WGR04_001159"
     CDS             complement(7850..8941)
                     /gene="ychF"
                     /locus_tag="WGR04_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ0794719.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERSMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(9055..10731)
                     /locus_tag="WGR04_001160"
     CDS             complement(9055..10731)
                     /locus_tag="WGR04_001160"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ0794720.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELDIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGGPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLADLKPDAAVVVATVRA
                     LKYNGGVERVNLGEENLDALAKGLPNLLKHISNLKNVFGLPVVVAINRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIENQPNNFKFAYDVELSIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            10906..11337
                     /locus_tag="WGR04_001161"
     CDS             10906..11337
                     /locus_tag="WGR04_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0794721.1"
                     /translation="MGSNAWLFWALASAGFASLTAVFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGHNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLNERPSTQEWIGLGLVTAGVLVLALKR"
     gene            complement(11583..12278)
                     /locus_tag="WGR04_001162"
     CDS             complement(11583..12278)
                     /locus_tag="WGR04_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ0794722.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEEALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGDAPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLADNPPHNPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(12294..12794)
                     /gene="hpaC"
                     /locus_tag="WGR04_001163"
     CDS             complement(12294..12794)
                     /gene="hpaC"
                     /locus_tag="WGR04_001163"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ0794723.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALVHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            complement(12909..13349)
                     /gene="nadR"
                     /locus_tag="WGR04_001164"
     CDS             complement(12909..13349)
                     /gene="nadR"
                     /locus_tag="WGR04_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ0794724.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(13692..14201)
                     /locus_tag="WGR04_001165"
     CDS             complement(13692..14201)
                     /locus_tag="WGR04_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ0794725.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            complement(14280..14355)
                     /locus_tag="WGR04_001166"
     tRNA            complement(14280..14355)
                     /locus_tag="WGR04_001166"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14320..14322),aa:Lys,seq:ttt)
     gene            14566..15645
                     /gene="apbC"
                     /locus_tag="WGR04_001167"
     CDS             14566..15645
                     /gene="apbC"
                     /locus_tag="WGR04_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ0794726.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIAAAVADSVQEILMPETGGAHIHLSIDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
BASE COUNT         3679 a         4418 c         4361 g         3731 t
ORIGIN      
        1 acgacgcggc gggaatctat cggaaatgac tgaaacctcg agattctaga ttcccacttt
       61 cgtgggaatg acggttcagt tgcattccga caacaccgca atcttgaaac ccctccgccg
      121 ttataaagac aaatcgcggc acaaaaaatg ccgtctgaaa tgctgttcgg cggtttcaga
      181 cggcatttgc tcaaacttta tcaggcgtaa tggcgcgttt cgccttctcc gccgacattc
      241 tctgcacagc gtttgcagac ggtttcatag cctgcaaccg cgcccacatc gcgggtgtag
      301 tgccagcagc gttcgcattt ttcgccgtcg ctggctttgg cggcaacggc aagttcatca
      361 cctactttca cttctgcttt agacaccagc aaggcaaaac gcaattcttc gcccaaagcg
      421 ttcaggtagc tggccatttc ttccggcgca gtgatttcgg cttccgcctg caaggacgaa
      481 ccgacggttt tgtcggcgcg caaaggctca atggcggcgg ttaccgcttc gcgggcttcg
      541 cggattgccg tccatttttt caccagttcg gcttcggctt tttcgttgat ggtcgggaac
      601 tcgtgccaag tatggaaaag gacgctgtct tcttcgccgc cgccgatgat gtcccacgct
      661 tcttcgccgg taaagcacaa aatcggtgca atcaagagaa ccaggctgcg cgtgatgtgg
      721 tacagggcgg tttgcgcgct gcggcgggcg cggctgtcgg ctttggtggt gtagaggcgg
      781 tctttcagga tatcgaggta gaacgcgccc aagtcttccg agcagaaaga aacaatgtct
      841 ttcacggcaa aatggaaggc ataacgcgga tagtaatcgc ctgccagacg ctcttgcagc
      901 cgacgtgcca ataccacggc gtagcggtcg atttccacca tatccgcctg ttgcacggca
      961 tcttcaatcg gattaaagtc gctcaagttg gcaaacaaaa agctcaaggt attgcggata
     1021 cggcggtagc tttcggttac gcgtttgagg atttctttgg aaatcgccaa ttcgccgctg
     1081 taatcggtag atgccgccca caggcgcagg atgtctgcgc cgaattcgtt ataaacctct
     1141 tgcggtgcaa cgacgttgcc gatggatttc gacatttttt tgccttcgcc gtcgacaacg
     1201 aaaccatggg tcagcagctg tttatacggc gcgcgaccca ttgatgaggc gcagccggtc
     1261 agcatggacg attgaaacca gccgcggtgt tggtcgctgc cttcgagata caaatcagcc
     1321 ggccattcca attcttcgcg ttgtttcaca acggaataat gggtcgagcc ggagtcgaac
     1381 catacgtcca ttgtgtcaga aagtttatcg taattttcgc aatcttccgc gctcaagagt
     1441 tcgctcttat cgagggagaa ccacgcttcg atgccttttt cttcgatttt ttgggcaact
     1501 ttttccaaca actcggcaga gttcggatgc aactcaccgg tttctttgtg aacaaagaaa
     1561 gtcatcggcg tgccccagta acgttggcgt gaaaccaccc agtcgggacg gccttcgatc
     1621 atcgcttcca aacgcgcgcg cccccaagac gggaaaaact cggtatcgtc cacggccttg
     1681 atggctttgt cgcgcagggt tttgccgtcg tttccggctt tgtccatacc gataaaccat
     1741 tgtccggtcg cgcggtaaat cagcggcgtt ttgtgccgcc agcagtgcgc gtagctgtgt
     1801 tcgattttgc tgcttgccaa gaggttgccg gtttcttcca gccattgcag gataacgggg
     1861 ttcgcctccc aaacgcgcat accggcaacg cgcggcgttt cgccgatata tttgccctcg
     1921 gcgttgacgg ggttgtaaag ctcgatgccg tatttgttgc agacggcata gtcttccaaa
     1981 ccgtgcgcgg gagcagtatg caccaagccg gtaccggcat cggtggtaac gtggtcgccg
     2041 ttaagcatag gaatatcgcg ttccaaaaac ggatggttca tatgcaggtt ttccagcttg
     2101 tcgccggtgg tttcggcaag gatggcgatg ccgtctgaaa agccgtagcg tttcaaagcg
     2161 tcttccgcca aatctttcgc cagcaccaat ttgcctttgg gcgtatcaat cagttggtaa
     2221 accacgtccg cgcccgccga tacggcttgg ctcgccggca gcgtccaagg cgtagtcgtc
     2281 caaatgacgg caaacgcttt gccctcgatg cctgccaaac cgaatgcggc ggcaagcgcg
     2341 gcagtgtctt taaacggata gcccacgtca atcgcaggcg atactttgtc tttgtattcc
     2401 acttccgctt cggccaatga agagccgcag tccaagcaga actgaaccgg ttttgcgccg
     2461 cggtagagat agccggattt gtagatttca ccaagcatac gcacggtatc ggcttcggtt
     2521 ttaaaatcca tggtcaggta aggacggtcc caatcgccca acacgcccaa gcggataaag
     2581 tcttttttct ggcgggcaac ttgttcggcg gcgtattcgc ggcataattc gcggaaacgc
     2641 gctttgggca tatctttgcc gtgcagtttt tccaccatca cttcgatggg caaaccgtgg
     2701 cagtcccaac ccggcacata aggcgcgtca aaaccggctt gggttttgct gcggataatg
     2761 atgtctttga gaattttatt gacggcatga ccgatgtgga tgtcgccgtt agcatacgga
     2821 ggaccgtcgt gcagaatgaa tttcggacgg cctttggcga tttcgcgcag tttttggtag
     2881 cgtttttgct cgtaccagct tttcagccat gcaggctcgc gcttggcaag attgccgcgc
     2941 attgggaacg ggctttcgag caggttgacg gttttgctgt aatcggtcat tttttaatct
     3001 ctattgttac aatatttcgg tttcagacgg cattgcgcct caaacagtat ttcacaacgg
     3061 gaaaccccta tgccgtctga aaagttaaaa gattgattgt agcccaatcg gatggtttgt
     3121 ataaggtttt tctaccaaca ctttgcggct tccatatcgg ctgctatctg ccttttcagt
     3181 tcttccatac cgtcaaactt ttcctcatcg cgcagtttgt gcaggaagcg gacgttcagc
     3241 cgttgtccgt acaagtcgcc ttgaaagtcg aacaagtgga cttcaagctt ttgagaacag
     3301 ccgctatcaa cgacggtggg attgaagccg aagctcgcca cgccgcgccg cgtgccgaat
     3361 gcgccgtccg cttcgacgac aaacacgccg ccgagtgcat aacggtggcg gggcaggcgg
     3421 atgttggcag tcggggcgtt taaggtgcgt ccgagttttc tgccgtgcac caccctgccg
     3481 ctcaagacgt agtcgtgtcc caaaagtttt ttcgcatagg caaggttgcc gtctgaaagg
     3541 gcttgtcgca cggcggtact gctggtgcgg atgtcttcga cgatgacgga aggcgtacgc
     3601 tcggtctgca tatcgggctg ttgtgccaaa agttcaaaac agccttcccg ccccgcgccg
     3661 aaacggaaat cgtcgccgac gagcaaataa cgcgtattca aggtttgacg cagcaggcgg
     3721 tcgataaacc cttgcgcgga tatttcggaa aaattttgat cgaaacgcaa aacccagacg
     3781 gcatcgacac agcctgtgcc ttccaataat tcgagcttgg tgcgcagggg gctgatccgg
     3841 cacggcggca tcctgccggt gcggagtgcg aaaaattctt tgggttgggg ttcgaaaacg
     3901 acggccacga cgggtaatcc gcgcgtatcg gcttcgaagc ggagtttttg gaggatgtgt
     3961 ttgtgtccga ggtgtacgcc gtcaaaattg cctatggtta cggcggcccc gtgcggaaag
     4021 tcgggcgagt tgtgccgccc cagcctgatt ctcattgtta tgttcgggta tgtggtgaaa
     4081 caggcggtca ttgtaaacgg tattgcggtt tatagacagt gtgccgcccg ttacgcccgc
     4141 ccgacgcggg aaaagtaggc aaatttcccg cagccgaacg cgccaaacgc ataaagcgcg
     4201 cagcaggcgc ggtgctatgt gttgaaacat cgccccaaac ctgatgcaac atcagtgaaa
     4261 atcgttcttt tttaaccagg taaaccgaga caaacaaccc tccgccgtca ttcccgcgca
     4321 ggcgggaatg acggcatttc tgcggcaatc ggattatttc caaaccaaaa gcgcgtggtt
     4381 gcgtttgccg cgccgaagga tggtgtattt gccgaaacgt ttgtgttcgt cggtcagcag
     4441 gcaggcatcg tcggggcgtt cggcggcgtg gttggggttg ttggcttcgg caggtttgcc
     4501 gttgagcaga accgctttgg cgttgacaaa gccgcgcgct tctttgttgg acgctgccaa
     4561 accggttttt accaaggctt cgacggcgtt gatgccgtct gaaatttcaa atgcgggcag
     4621 gccgtcgagg gcgagctgct cgaagtcgct ttcggtcagg ctgctttggt cttcggcaaa
     4681 caggctttcg gaaatgcgct gcgcggcggc aagggcttct tcgccgtgaa tcaggcgggt
     4741 catttcttcg gcgaggatgc gttgcgcttc gggcttgctg ccgctggctt tatctttcgc
     4801 ttcgatagcg tcgatttctt cgatggacag gaaggtaaag tatttcagga atttatacac
     4861 atcggcatcg gcgactttca gccagaattg gtagaactga tagggcgagg tttttttcgc
     4921 gttcagccac accgcgccgc cttcggtttt gccgaatttg gtgccgtctg atttggtaac
     4981 caaaggcaga gtcagtccga acacttgttt ttggtacagg cggcgggtca ggtcgatacc
     5041 ggcagtgatg ttaccccatt ggtcggagcc gccgatttcc aaaaccgcgc cgtggcgttt
     5101 gttcaactcg gcgaagtcgt aaccttgcag cagggaatag gcgaactcgg tgaaggaaat
     5161 gcctgcgccg tcgcggtcga tgcgctgttt gacggattct ttgttcagca tggcgttgac
     5221 ggagaaatgc ttgccgatgt cgcgcaggaa gtcgaggcag ttcattttgc cgaaccagtc
     5281 ggcgttgttc gccataatgg cggcgtttcc gccttcaaag ctcaagaaag gggttaattg
     5341 gctgcggatg ctttccaccc agccggcaac ggtttcggcg gaattcaagc tgcgttcggc
     5401 ggctttgaag ctggggtcgc cgatcatacc ggtcgcaccg cccaccagtg caatcggcgt
     5461 atgccccgcc tgttggaagc ggcgcaatgc caatacgggc agcaggtgtc cgatgtgcag
     5521 gctgtcggcg gtcgggtcga agccgcaata aagggcaatt ttttgttcgt tcaacaaagc
     5581 gtctaaggct tcgatgtcgg tggtttgcgc gattaggccg cgcgattgca ggtcttggat
     5641 gacgctcatc ggtctctttc aaaaaaaatt agcggttttg caaaccgccg attgtaacaa
     5701 atttaagcga atcaatggtt atggcgcgta tcgagaaacc gttgtttttc ggaaaaacgc
     5761 ttcgccaatt ccgtgccgcc gtaagggttg atgtggtctt tgtccgagta aaccggcaat
     5821 ccgccgattt ggaaatttgc ggggatatag gcggcggcat caataatata gacgttgggg
     5881 tatttggctg ccaattccct gatgcgtgca ttggctttca gagtgctttc gtcgtccggg
     5941 cgcagggttt ggcggtaacc cggtatgcgt gaagacaaga tataggcgcg ttgtacgttg
     6001 taagacgagg caaggttgtc cgccatcagg taaacggctt gtttttcgga tgagagtttg
     6061 tgcagcatac ggtcgaattt ttggaaaaaa ccggcatcgt aggcaaggga gcggctgttt
     6121 tcgggcattt ggctgcccca gcgcatcgcc aaaaccactt ttgaatacag gggcaggtgt
     6181 tcttcggcat agcggtaaac ggcgcggcag gcggcccagt tttggaacac acgggacgcg
     6241 tagccttcca cataggcgca agcgtcggcg gaaaccatag tggcggacca tttttctttt
     6301 ttgcccacgg catcgaagaa tgttttgtaa tggtcggcgt gggagtcgcc caaaaccagc
     6361 agctccggct gtttttccgt atccccccac aggcattgtt tgccggtatt gttgtggcag
     6421 gaggtgttgg aacgcgtcag ccccaagcgg tcgtattgcg ccataaacgg cagcctcatc
     6481 gcgaaaaacg agcccgcccc caaaatgagc ataggcaagg cataaatcca taaaacggat
     6541 tgtgcgaacg aacctttcca ttttttaaac ggcttttcga tgcagtgata agaaatcagg
     6601 gaaagcagca atatcaggac gattgctgcc gctgttgaat aaggcggcag gttgtccggg
     6661 ccgatatagc gcataaaggc caatatcggc caatgccaca gataaagcga ataggaaatc
     6721 aaaccggcgg caacggtgat tttccattgg aaaaattttt taagcgggtg ttcgtaatga
     6781 ttgaaataaa tcagcgcggc aacagccaga cagggaatca aagcggcggg gcccgggaaa
     6841 taggcggttt gttccgaata ggaaaacagg caggttgaca atatgcacac ggcaaacaat
     6901 gcgccggcgg cggcacagcg tctgccgacg gcaggttgcc ggcagcgcat ccacacggcg
     6961 gtcagcgatc cgaccagcat ttcgcaggcg cgcaggtggg gcaggtaata tttatcgaac
     7021 gcggaaggca taaaggaagc ggcaaggctt aaggcgcaca gtgcggcgag gaagccgaac
     7081 tgtacgcgca ggcttttgcg ggcgacaagc agcagcagta tcggaaagac aaagtaaaat
     7141 tgttcttcga ccgacaaaga ccagatgtgc agcaggggct tttcttcctg cgcgggatcg
     7201 aaataatcct tcccccttgc aaaatacagg ttagaggcga aacccaaggc ggtcagcgcg
     7261 gatttccaca aaagaaagaa atcatctttg gtgaataaaa agaagccgcc tgccagcgtt
     7321 gccgccaata cggcgaaaaa tgcgggcaga atccgcttga tgcggcggat ataaaatgcc
     7381 ttcagggaaa acctcccccc ccccgacatt tcgcggtgaa gaatcgtcgt catcaaaaag
     7441 cctgaaatca caaagaatat atcgacaccg agaaacccgc ccggcagcca atccttttcg
     7501 atatggaaca cgatgacgga caagacggcg gcggcgcgca atgtgtcgat gtccgggcgg
     7561 tagggtaagg cttggctcat aatatttttg ctttcatcgc ggcggcgcaa atgccatctg
     7621 aaaggtcatt ttgtatcggg gctcgggata ttcggaatgc cggacggatt tttgcaacag
     7681 cggggcaggc ggtttgtttt gcaggaatcg gggagggcaa atcggaaatg cggacgggat
     7741 tttattttga tgcagccgca ttccggcggt atggaaacgc cgaaaaccac caaaaccggc
     7801 ttcagacggc atttctggca aagccgtctg aaaacctgcc gcatttgggt tacacgttaa
     7861 acaaaaagtg catcacatcc ccatcctgca cgacgtattc cttaccttcc acacgcattt
     7921 tgccggcttc tttggctttg gcttcgccgc cgagcgagac aaagtcgtcg taagaaatga
     7981 cttgggcgcg gatgaagccg cgttcgaagt cggtgtgaat cacgccggcg gcttgtgggg
     8041 ctgtgtcgcc tttgtgtatc gtccacgcgc ggacttcttt gacgccggca gtgaagtagg
     8101 tttgcagccc caagaggtcg taacccgcgc ggatgaggcg gttcaggccg ggttcttcca
     8161 aacccatttc tgcgaggaat tcggcttttt cgtcgtcttc caattcggcg atttcgcttt
     8221 ccatcgcggc gcatacggcg acgacggggg cgttttcttt tgccgccaat tctttcaggc
     8281 ggtcgaggtg cggattgttt tcaaaaccgt cttcggcgac gttgcccaca tacatcgcag
     8341 gtttggcggt cagcaggaac agcggcttga gcatcgagcg ttcttccgcg tccaaaccga
     8401 aggaacgcac gggtttgcct tcgtccagat gcggcaacag ttttttgcac agatcgacca
     8461 gtttttgcgc gtctttgtcg ccggagcggg cgcgtttttc ttcgcggacg atggcttttt
     8521 cgacacttgc taggtcggca agcgccaact ctgtaccgat ggtttcgatg tcggcaatcg
     8581 gatcgacgcg gcctgcaacg tggacgatgt tgtcgtcgtc aaagcagcgt acgacgttaa
     8641 caatcgcgtc agtctcgcgg atgttggcaa ggaactggtt gcccagacct tcgcctttgc
     8701 tcgcgcctgc aaccaaaccg gcgatatcga caaattcgac gatggcaggc tgcatttttt
     8761 gcggattgac gatttttgcc aattcagcca tacgcgggtc gggcacttcg acgatgccga
     8821 cgttgggttc gatggtacag aaaggatagt ttgccgcttc gatacccgac tgggtcagcg
     8881 cgttaaagag ggtggatttg ccgacgttgg gcaaaccgac gatgccgcat ttcaaactca
     8941 tgttttttcc tgaaaataga gaaatttaac ggcggattat agcataccgc cgcccgcgtt
     9001 ccgaaaaaat gccgtctgaa acggcttcag acggcatccg gtttcaaaaa ccgttcagaa
     9061 caagccgtga atcacgccgt gttcgtccac atcgattttc tcggcggccg gaactttcgg
     9121 cagacccggc atcttcatca tattgccgca cagcgcgacg atgaagcccg cgccggcgga
     9181 aacggtgatg ccgcgcacgg cgatgcggaa gccttcgggg cagcccaaca gtttggcgtt
     9241 gtcgctcaaa gagtattggg ttttcgccat gcagatcggc attttgtcca aacccagttt
     9301 ttccagcgag gcgatttcgg cagacgcttc cgcgctgaaa tcgacatctt ccgcaccgta
     9361 cactttttgg gcaatcgcac ggattttgtc tttaatgctc aactcaacat cgtaggcaaa
     9421 cttgaagtta ttcggttggt tttcgatggc gttgacgact ttgcgcgcca agtctgcacc
     9481 acccgcgcca cctttgcccc atacttcagt cagggaaact tcaacgccgt gttccgcgca
     9541 ggctttttca atcatcgcca actcggcatc ggagtcggac acgaagcggt tgatggcaac
     9601 caccacaggc aggccgaata cgtttttcag gttggaaatg tgtttcagca gattaggtag
     9661 gccttttgcc aaagcgtcga ggttttcttc gccaaggttg acacgctcca cgccgccgtt
     9721 gtatttcaaa gcgcggacag tagccacgac aacagccgca tcaggtttca aatccgccag
     9781 acggcatttg atgtcgcaga atttttccgc acccaaatcc gcgccgaagc ctgcttcggt
     9841 tacggcgtaa tcggcaaggt gtttcgccaa acgggtggcg gtaacggagt tacagccgtg
     9901 ggcgatgttg gcgaacgggc cgccgtgtac aaatgccgga gtgccttcga tggtttgcac
     9961 caagttaggc ttaatcgcat ctttgagcag ggccgccatc gcgccgtgtg ccttcaaatc
    10021 tttggcgtaa acggggccgc cgtctttggc gtaggcgaca aggatgttgc ccaaacgctc
    10081 tttcaaatca ctgatgtctt tggcaagaca gaataccgcc atcacttcgg aggcaacggt
    10141 aatatcgaaa ccgtcgggac gcatcacgcc gtcaacaggt ttgcccatgc cgtcgatgat
    10201 gttgcgcaac tgacggtcgt tcatatcgac cacgcgccgc cacagcacgc gtttggggtc
    10261 gatgtccaac tcgttgccct gatagatatg gttgtcgagc atcgcggcaa gcagattatt
    10321 tgccgcaccg atggcgtgaa aatctccggt gaagtgcagg ttgatgtctt ccatcggcaa
    10381 aacttgggca tagccgccgc ctgccgcgcc gcctttcacg ccgaacaccg gccccagaga
    10441 aggttcgcgc agggcaatca cggcatcttt gccgatgtgg cgcaacgcgt ccgccaaacc
    10501 gatggttacg gtggttttgc cttcgcccgc cggagtcgga ttaatggcgg taaccaaaat
    10561 cagcctgccc tgtttttgcg gcagtttgaa cgcttcggca ggattgattt tcgccttgta
    10621 atgaccgtaa ggctcaatgt tgtcggcatt cagacccagc ttggcggcaa tttcgccaat
    10681 cgggcgcatg gtggaggatt gggcgatttc ggcatcggtt ttgaagctca tgattttcct
    10741 ttagaaatga ggagggacat gccgtctgaa agcatcaggc gacaaacagg tggattgaaa
    10801 ataatatcag gcatattata acgttatccg caccaaaccc gcagtgaaat ttttgacgct
    10861 gcaacaaaaa taccgttcat attgttcaca atccaaggag aaaacatggg cagcaacgca
    10921 tggctgtttt gggcattggc atcggcaggc ttcgcctcat tgaccgccgt tttcgccaaa
    10981 atgggcttgc agggtataga ttcagatttc gctaccttca tccgcacctt ggtcatcctc
    11041 gccgctttgg tactgttttt gacctacacc ggcaaatggc agggcgtgaa cggctttacg
    11101 gggcacaact ggacattcct tatcctatcc ggccttgcta ccggcgcatc ttggcttgcc
    11161 tacttcaaag ccctgcaact gggtaacgcc tcgcaagtcg cccccgtcga taaattcagc
    11221 ctggttctgg tcgccctgat ggcagtagtc ttcctcaacg aacgccccag cacgcaggaa
    11281 tggataggct tggggctggt aacggcgggc gtgttggtgc tggcgttgaa acgttaaacc
    11341 gaatccgcca taccgcctga aaccgggttt tgacaatctg acgggcggcg gtctgaacaa
    11401 agttacccaa taaaatccga caccgcgccg acggcaaacc gccgcatccg acaggggtcg
    11461 gcttcagatg gcatttgttg gacggaaagc cccgaacgcc ctattttcac gcctccacga
    11521 aaatatgccg gacatccggt cggaaccgtt cggcagccgg ctcggcaagc caccgtttta
    11581 tttcacttcc aagcccctgc aagggcttga gcctctttca gacggcatac cgtgccgaca
    11641 tccagccaca agcccgtatg cttctgtccg ctgacgcggt tttgccgcat ttcgccccgc
    11701 aatacgggcg cgagtttcgc cacactgccc gcttcgattc catcaaacat ttcaggacgg
    11761 taaataccca cgccgctaaa tgtcaatccg ttgccgccat ttacttccgg ccgcacgctg
    11821 ccgtcgggca gcagggaaaa atcgccgtcg gggttgtgcg gcggattgtc cgccagccac
    11881 agatgggcgg aaatgtgctc gggcagggac gatgccgcct gaaacgcggc ggtaaaatcg
    11941 atgtcggtca gcacgtcgcc attgacgacc aaaaacgggg catcgcccaa cagcggcaat
    12001 gcctgcgcga tgccgcctgc cgtttccaag ccgcctgcgg gttcgggcga ataggcgatg
    12061 ttcacgccat aagccgagcc gtcgcccaaa gcctcttcta tctgccgacc taaccaagca
    12121 tggttgatga cgatttcggt aaaccccgcc tgcttcagac ggcataggtg ccaaccgatt
    12181 agaggcttac ccgccacatc gagcagcggc ttcggagtgg tatcggtcaa agggcgcata
    12241 cgctcgccgc gtcctgccgc cagtatcatc gctttcatat atctgtccga atatcagtct
    12301 aaaaatctaa actgccgtct gaaatacagc agcgcggggc gtttgcaccc gcagtttttg
    12361 atttcgtcga gcctgacgta aaacacaaaa tgcgtgccga tttcatgttt gccgacaata
    12421 tgcccgtgca ggtgcaccaa cgcgccctct atttcaagtt gtcccgtttt gccgcgatgc
    12481 cagatgtggt aggcaaaccg ctcttcgggc gacaggccgg tcagcccggc aaaatgttcg
    12541 gcaacatcct gatgttcgtc cgccagcgta ttgatgcaga ggctgccgtt ttccgacagg
    12601 atcggaatga ttcgcgcact ccggttgatg cacagcatca cggtcggcgg ctcgtcggta
    12661 accggcgcaa ccgccgtcat tgtaatgccg taacgccctg ccgcaccgtc tgtcgtgatg
    12721 acatgaacgc ctgccgcaca agatgccatc gcatcacgga acgaagtttg aaaagttttc
    12781 tgcaaatccg ccatttttcc cctttaaact gtcccctata taagaatgct gcacacaagg
    12841 caccccccat gtgcagcagt tctgattcaa aaagccgtcg gtcggacatt tccgcgcgtt
    12901 acggcgtatt acgagttcaa cgcatcctcg attttggcaa gttctgccaa caggtcttta
    12961 agcagcagca ttttctcgcg gcccagcact tcctcgatag cgtcgtaacg ctcgtccact
    13021 tcttcgccga tttcctcata cagcttctcg ccctcggcag tcagcttcag aaaaacacgt
    13081 cgttggtcgt tggaaggttt caggcggaca accaaacccg ctttttcaag gcgggtcagg
    13141 ataccggtca ggctggggcg cagaatacac gcctgattcg ccaaatcttg aaagtccagc
    13201 gtgccgtttt ccgccaaaag acggataatc cgccattgct gatcggtaat attcgcctga
    13261 ttcagaatag gcctgaattg ggtcatcagg gcttcccttg cctgtatcag actgatattg
    13321 atagacgcat gttttgattg ggtaggcatt gttcaagtct ccaagttatc gaaaatcaaa
    13381 ctttcaaacc gtcgggaaag cctgtgggcg taaattttga tgcaaccgtt atataacaaa
    13441 acgaacatat agcaacaata cgctataaac cgcatcggac gactgggtat aaaagacttt
    13501 aattccgata atcctatcta aaaatatttt aatagttata tcttagtcta ttttccctac
    13561 aatctcaaca aagtattaca tttactgtcg aaagaacttt cttccagaaa aaacaaaagc
    13621 cgccgcatca gacgagcaag gtatatgccg tttgattctc tacatagcgt aaagtttaac
    13681 aaaaacaaaa gttaaccgaa aacatccgcc tgaaaaattc gtgcgcgcaa gccccaataa
    13741 ctgctgattc ccgtcgtata gtgaaccatt ttcccatttt tgaccaaaac gacggcaggc
    13801 gttgcgacaa tccgccaaga ccttgccaaa cccccgtcct catcgttgac cgtcggaaag
    13861 cccaagcccc gctttgccat atacgccgcc acttcggacg cgctgccgga acgtaccgcc
    13921 acgccgacga ccggcacgcc gtccgccgcc aaatcatcga ttatcggcga ctgataacgg
    13981 cacacgccgc accagctccc ccaaaaatac accaaaaccg ccttatctcg gctaaactgt
    14041 cccaaagtca gccgctgccc cgacagcagg gtcaaaggcc gccctgccgc accggcaggt
    14101 tcgtcgggct tgcgtatcca atccaaaaac agcgacacca ataaaaacac caatgccgtc
    14161 tgaacggcaa atttgatgcc cgaaagcagt ttctttttca tacgctctct caaacggtac
    14221 gcccacgcaa ccggcaggca aacaaaaagc caagtctcaa aacttggctt ccggttatct
    14281 ggtgggtcgt gagcgattcg aacgctcgac caacggatta aaagtccgct gctctaccgg
    14341 ctgagctaac gacccgataa gccgtgcatt atacagcacc atcctacctc gtcaagcaaa
    14401 ttttacaggc ttaattgcag accactgttt gcacgggata ttttgacaac ggattttcac
    14461 aatccgccgc ataccgtgta aaagttcgca caaggaaaag caaaccgccc gaaatcaatg
    14521 tacactttcc gcccgtttcc caaactgcac acagaaacac acattatgaa catacaaaac
    14581 atccgcaccc tcctcgacac cgtcgccgtt ccgaatacgg cacgcacgct cggcggcgaa
    14641 aaggccgtcc gttcggtcga acagcgttca gacggcatcc atatcgccct gcatttcggc
    14701 ttccctgtcg cgcacatcgc cgccgccgtc gccgattccg tacaagaaat cctgatgccc
    14761 gaaacaggcg gcgcgcacat ccatctgtcc atagacaccg aaatcggcac acataaagtc
    14821 caacctggcg ttaccaccat caaaggtgtg aaaaacatca ttgccgtcgc ctccggcaaa
    14881 ggcggcgtgg gcaaatcaac aaccaccgcc aaccttgccg ccgcaatggc gcggatgggc
    14941 gcgcgcgtcg gcgtgctcga tgccgacctt tacggcccga gccaaccgac catgttgggt
    15001 gtggacgacc gcaaacccga tcagaaaaac caaaaactca ttcccgtcga atcttcagac
    15061 ggcatacagg tcatgtctat cggctttctc gtcgataccg accaagccgt cgtctggcgc
    15121 gggccgatgg tcagccaagc cttgcagcaa ctgatgtttc aaagcgagtg ggacgaagtg
    15181 gactacctgt ttatcgacct gccccccggc acgggcgaca tccaactcac actgtcccaa
    15241 cgcatccccg ttaccgggtc cgtcatcgta accacgccgc aggacatcgc gctgatagac
    15301 gcgcgcaagg ctgtggatat gttccgcaag gtcaacattc ccattttagg cgtattggaa
    15361 aacatgtccg tccacatctg ctccaactgc gggcatagcg aagcactgtt cggcacggac
    15421 ggcggcaaag atttggcggc ccgcctcaac gtccccctgc tcggacagct tcccctaagc
    15481 ctgcccgtgc gcgaagccat ggacggcggc acaccggcgc aactgttcga cgaacacccc
    15541 gccatcgccc gaatctacac cgatgccgca ttccaaatcg ccctgggcat tgccgacaaa
    15601 ggcaaagact tcagcagccg cttccccaaa atcgtcgtcg aataaagccg cgtccgaaac
    15661 cgcaacagca atgccgtctg aagccccgcg cctgccggcg ggcaaacttg ccggataaaa
    15721 cggttttttg agattttacg ttccggattc ccactttcgc ggcaatggcg ggattttagg
    15781 tttctgtttt tggttttctg tttttgtagg aatgatgaaa ttttgagttt taggaattta
    15841 tcggaaaaaa cagaaaccgc tccgccgtta ttcccgcgca ggcgggaatc cagaccttgg
    15901 gataacagca atattcaaag gttataaaag acccgtcatt cccgcgcagg cgggaatcta
    15961 gaccttagaa caacagtaat attcaaagat tataaaagac ccgtcattcc cgcgcaggcg
    16021 ggaatctaga ccttagaata acagcaatat tcaaagatta tctgaaagtc cgagattctg
    16081 gattcccact ttcgtgggaa tgacgggatg taggttcgtg ggaatgacgg gatttgagat
    16141 tgcggcattt atcggaaaaa acagaaaccg ctccgccgtc attcccgcg
//
LOCUS       DAWFJR010000040        15881 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWFJR010000040 DAWFJR010000000
VERSION     DAWFJR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15881
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.040"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(273..1787)
                     /gene="katA"
                     /locus_tag="WGR04_001168"
     CDS             complement(273..1787)
                     /gene="katA"
                     /locus_tag="WGR04_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ0794727.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITQYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPASYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRSQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQAGLL"
     gene            2135..2986
                     /locus_tag="WGR04_001169"
     CDS             2135..2986
                     /locus_tag="WGR04_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HEZ0794728.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSY
                     QEAACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLQHELTALKKFDPAGVAA
                     ADLNESLILQIERLGECAAKPAALHIVRNALDSIDGNRSQTLARIKKHLPQTDSGTLE
                     AALDLIASLNPFPAAGFALSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAA
                     HKRKTAE"
     gene            complement(3455..4584)
                     /locus_tag="WGR04_001170"
                     /pseudo
     CDS             complement(3455..4584)
                     /locus_tag="WGR04_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            4597..4737
                     /locus_tag="WGR04_001171"
     CDS             4597..4737
                     /locus_tag="WGR04_001171"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794729.1"
                     /translation="MNYRIGNAVCIDEMPDTGRGGQTFAKRTASDASPARAGAASDGI
                     LS"
     gene            complement(4940..6157)
                     /locus_tag="WGR04_001172"
     CDS             complement(4940..6157)
                     /locus_tag="WGR04_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0794730.1"
                     /translation="MTHTASKTPKFWLTITAAAFILLITIGMRMTLGLFVQPVVNTTE
                     LSIAQFSLIIAVFQLMWGISQPLSGALADRFGAFRVLSGGAVLLVCACLIAPNIPTYW
                     GLMIAVGLLLAFGTGSGGFSIIMGQVAAQVPAHKRGLASGLVNAGGSAGQFLFAPLVQ
                     GLVVLPEVGWTGTFYVWGAIALLILPVSWWLAGGNNAAHTQHAQATHGQSLGEAVKTA
                     FKTPSYILLHLSFFACGFHIAFLVTHLPTEVALCGLPATVASTSIAIIGLANIAGCIF
                     SGWCTSRVRGKYVLFGLYASRAAMILIYIFSPKTDLNFYIFAAALGFTWLATVTPTAS
                     ITGKLFGTRYLATLFGLTMLSHQIGGFLGSYIGGIVITQFGDYGWMWYADALLAGTAA
                     LLNLPIREPRTAA"
     gene            6585..7538
                     /locus_tag="WGR04_001173"
     CDS             6585..7538
                     /locus_tag="WGR04_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ0794731.1"
                     /translation="MGFLIGLSRNLTRFTALVILLASAVAFIGPASFAWVKGDVQVLV
                     LGIIMLGMGMTLGREDYRILAQRPLDVFIGAAAQYTVMPLLAIGIAKLFDLSPGLTLG
                     LVLVGTCPGGVSSNIMSFLAKGDVAFSVGMTTVSTVLAPAVTPLWMTCLVGQTVDMDG
                     WGMFRFMLLVTLLPVVLGSAANILLHGKHWFENVRAVMPGVAVLAFACIVGGVASVHG
                     HRFAESALVMVLAIAVHNIAGYVSGYYAAALFGMDTAKKRTLAIEVGVQNAGLATGLS
                     AKFFPGNPESAVAAAVACVWHSVSGTVLGNLFALWDKRRDA"
     gene            complement(7780..9027)
                     /gene="fabF"
                     /locus_tag="WGR04_001174"
     CDS             complement(7780..9027)
                     /gene="fabF"
                     /locus_tag="WGR04_001174"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ0794732.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(9183..9419)
                     /gene="acpP"
                     /locus_tag="WGR04_001175"
     CDS             complement(9183..9419)
                     /gene="acpP"
                     /locus_tag="WGR04_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ0794733.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            9651..10658
                     /locus_tag="WGR04_001176"
     CDS             9651..10658
                     /locus_tag="WGR04_001176"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ0794734.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNMVYKLGLIPVTGNRTKPV
                     KLMGLDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPNPQPGNPQPRLFRVPE
                     YQGIINRMGFNNHGIDAMIQNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVHGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGGDAAEKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            10991..11314
                     /locus_tag="WGR04_001177"
     CDS             10991..11314
                     /locus_tag="WGR04_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794735.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            11401..11682
                     /locus_tag="WGR04_001178"
                     /pseudo
     CDS             11401..11682
                     /locus_tag="WGR04_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11735..12208
                     /locus_tag="WGR04_001179"
     CDS             11735..12208
                     /locus_tag="WGR04_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ0794736.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            12462..12797
                     /locus_tag="WGR04_001180"
     CDS             12462..12797
                     /locus_tag="WGR04_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794737.1"
                     /translation="MTVDKEQVEKMIYSENKQSVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLLSKQGDPDFQGFAEDALDEI
                     SLFIFKKRP"
     gene            12972..15662
                     /gene="glnE"
                     /locus_tag="WGR04_001181"
     CDS             12972..15662
                     /gene="glnE"
                     /locus_tag="WGR04_001181"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ0794738.1"
                     /translation="MSDNRLDTARHHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFTYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNGIKSLVRPFVFRKYLDYSAYEAMRKLHRQISS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAETGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDQQTQTLPASPEQQQLLAESMGFDSYAAFSDDL
                     NAHRTKVNQLFNEILSEPEEQTLDTSEWQWAWQEKPDEEERQGRLKEHGFDAETIAAR
                     LDQIRNGHKYRHLSAHAQLRFDAVVPLFVQAAAEQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DGIEACGGDTEAQMDALRRFQHAQVFRLAVQDLAGLWTVEPLSDQLSALADTILAAAL
                     PCAWADMPKKHRDTPQFAIVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSTATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEMLTAERDQTVLAGEIIEMRERMFPTHPPVDSNVKYAHGGV
                     VDVEFIVQYLILAHARKYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWREVFGEEAATA"
BASE COUNT         3691 a         3944 c         4440 g         3806 t
ORIGIN      
        1 ctttcgcggg aatgacgcgg aaaagttttc gtgccggttt gaaccggtgg ggatggtgcg
       61 ggaatgacgg cggaaaagtg ggctgtgggg cgggaatgac gggatatttt gcgtttaata
      121 aaaaacgccc gctgaaacgg cgggggcggg atggggaaat gccgtctgaa gcggtcaaac
      181 gacgtttcag acggcatttt tatgcccggt tatttccggt agcggacggc gcgggacagg
      241 atttcttcaa tttccatcca cataatgccc ccttacagca aaccggcctg acccagtgcg
      301 gggtcggtcg cgcgggcggc ttgtgcgtct tcgacagtca gtccgagggc ttttgccacg
      361 ccttcgccgt atgccggatc gcaacggtag cagttgcgga tatggcggta tttgatgaag
      421 tcgggcgcgt cgcccattgc ggcggcggtg ttgccgaaca atgcctgttt ctgcgcgtcg
      481 ttcatcaggt tgaacagggc gcgcggttgg ctgaaatagt cgtcatcgtc ttggcggtag
      541 tcccagtgcg ccgcgtcgcc gttgattttc aaaggcggtt cggcgaagtc gggctgctgc
      601 tgccattggc cgaagctgtt gggttcgtag tgcggcaggc tgccgtagtt gccgtcggcg
      661 cgtccttgtc cgtcgcgctg gttgctgtga acagggcaac gcggacggtt gacagggatt
      721 tggcggaagt tcacgcccaa gcggtagcgt tgcgcgtcgg cgtaattgaa caaacgcgcc
      781 tgcaacattt tatctgggct ggcgccgaca ccgggaacga ggttgctcgg tgcgaaggcg
      841 gattgttcca catcggcgaa gaagttttcg ggattgcggt tcaactcgaa ttcgcccact
      901 tcaatcagcg gatagtcttt tttcggccaa actttggtca agtcaaacgg atgatacggc
      961 actttttccg catcggcttc aggcatgact tggatgtaca tcgtccattt cggaaactcg
     1021 ccgcgctcga tggcttcgta caggtcgcgc tgatggcttt cacggtcgtc ggcgatgatt
     1081 tttgcagctt cttcgttggt caggttttta atgccttgtt ggctgcggaa atggaatttt
     1141 acccaaaagc gttcgcccgc ttcgttccag aagctgtaag tatgcgaacc gaagccgtgc
     1201 atatggcggt agctggcggg aataccgcgg tcgctcatga cgatggtaac ttggtgcaac
     1261 gcttcaggca gcagcgtcca gaagtcccag ttgtttgtgg cggaacgcat attggtgcgc
     1321 ggatcgcgtt tgacggcttt gttcaggtcg gggaacttac gcgggtcgcg caggaagaat
     1381 acgggcgtgt tgttgccgac cacatcccag ttgccttctt cggtatagaa cttcaaggcg
     1441 aagccgcgga tgtcgcgttc cgcatcggct gcgccgcgct cgcccgccac ggtggtgaaa
     1501 cgggcgaaca tctcggtttt tttgccgact tcgctgaaaa ttttggcgcg agtgtattgg
     1561 gtgatgtcgt gcgttacggt aaacgtaccg aacgcgcccg aacctttggc gtgcatacgg
     1621 cgttcgggaa tgacttcgcg cacgaagtcg gcgagttttt cattcagcca caaatcctgc
     1681 gccagcagag ggccgcgcgg gccggcggtc aggctgtttt gattgtcggc aacaggcgcg
     1741 ccgttgttca tggtcagatg ggttacgggg catttggagg tagtcatcgc tcttgttcct
     1801 tttctcaggt tggtcaaatg ggggtaaacg gcttacagta cgatttggcg gaaagcgtat
     1861 tcgtaaccgg tttcttgatt gcaataaatt tcttgaatcg acattttgtt ttccttttgt
     1921 aaaaactatg gatgcgacta tacgccaaga ttttcgctat taaaactatg aaatcgattt
     1981 aatattgtta taagcaatcg gtttttgatt ttcgtttgtt ttttgttatc gaacggaatc
     2041 cgaacccgcc cattaaaacc atttataatg caatgacgct ttgcggcatt ttttgcgccg
     2101 acaggctgaa aataacaatt tcccccacat tatcatgacc ttactcggaa taaagctcaa
     2161 acagacccag cagctcaacc agcggctgca acaatctttg cgcgtattgc agatgtcggg
     2221 tatcgaactt gaacgcgagg tcgaaaactg gctgtcggac aaccccctgc tcgaacgcaa
     2281 agacacggat gaattttccg atgccgagtt cagccattac actgcgcctg cccgtcaaat
     2341 cggcggagac gaaggcgaag atatgctgtc caacatcgcc ggcgagcagg atttcaagca
     2401 atacctgcac gcgcaagtgt gcgaacaccc gctttcctac caagaagccg cctgtgtcca
     2461 catccttatc gatttccttg acgagcaggg ctacctgacc gacagcatcg aagacatcct
     2521 cgaccatacg cccttggagt ggatgttgga tgaagcaatg ctgcaacacg agctgaccgc
     2581 attgaaaaaa ttcgacccgg caggcgtggc cgccgccgat ttgaacgaat cgctgatact
     2641 gcagatagaa agattgggcg aatgtgctgc caaacccgcc gccctgcata tcgtccgaaa
     2701 cgccctcgac agcattgacg gcaaccgcag ccaaaccctc gcacgaataa aaaaacacct
     2761 gccccaaacc gacagcggca cactcgaagc cgcactcgac ctcattgctt cgctcaatcc
     2821 ctttcccgcc gccggttttg ccttgtccac acccacgccg tattctgacg aggcgctcgc
     2881 caacctgctg gctttccgcg gcatggaggt ttctcgccgc accattgcca aatacagaga
     2941 atcctttgag attccggcag cacacaaacg caaaaccgca gaataattgc cgaataatct
     3001 tataaagaca acaaaccaaa agccggcatt tctgcgaaag cgggaatgcc gaatccgtcc
     3061 gcgcggaaac ctgcatcccg tcattcccgc gaaagaggga atctagaaac gcaaagttgc
     3121 aagaatttat cggaaatgac cgaaactcaa cgaacctgga ttcccgcttt cgcgggaatg
     3181 acgagggttt gggatttctg tttttgaatt tctgtttttg tgagaatggc aagattttcg
     3241 gttcttgtat ggataacgag attttagatg gcgggaattt gtcgggaaaa cagcaaccct
     3301 ccgccgtcat tcccacgaaa gtgggaatct agaaacgcaa agttgcaaga atttatcgga
     3361 aatgaccgaa actcaacgaa cctggattcc cgctttcgcg ggaatgacgg gggtttatca
     3421 gaaatgaccg aaactcaaaa gcgggcagcc ttgtttacgc cttcaaaata tcgagcaatt
     3481 tcaaatcgac tttttcggca tcgaatttat ctttggcaat cgcataactt gcattcccca
     3541 tcaggcggac ggcttccctg ttttcgataa aataaatcat tttttcggct aagatgcggg
     3601 gattccaagg ctcgatcagg aagccgttga ccttgtcggc gaccgtttcc ctgcatccgg
     3661 ggacatccgt cgtaatcact gccctgccga cggccattgc ctcctgagtg cttcggggaa
     3721 cgccttccct ataagacggc aatacgaata tatgatgcgc ctttatcact tcggaaacat
     3781 tgttcacaaa accggggaaa cggataatat cgcgggcggc aagccgttcc aaatcgcccc
     3841 cccccccccg cgtgatttgt cgattgcgcc caaagcggta aaaaccgtat cggggtattt
     3901 gtccttaacc tgttccgccg cccgaataaa atcatcaatc cccttttctt tcagaaatct
     3961 gccgataaag aggaatttta cgggttcttt ttcatcggga atatccgcct cggaataagg
     4021 atattgccgc gaatccagac cgattccgcc caaaatatgg atgtttttta ttttgatgcc
     4081 gtatttgtcc gtcagttcgt ctttgtcgtc ggggtttaat acaatcaggc tttccaacat
     4141 cggcagggca atgtggtaca aggcaatcaa aatccccttt atgatttttg ttgttaacgg
     4201 tatgccttcc ggctgcgggg taaatgcgaa tcccaaacct tccagcatcc cgacgattct
     4261 gggcacgcct gccagttttg cggcaaaagt gccgaaaatc acgggttttg cgaaataagg
     4321 gaaaaccaaa tccggcgata tttttttgag ttctttaaat atgaggaagg tggattttat
     4381 atcggaaaac gggttcagcc cgctgcggtt tgaacggtag gtaacgggtg taacccccat
     4441 ttccctgata atatccaatt cattatcgga aaactccgat acaaaggcat acacctgatg
     4501 gtttttgccg attaattttt taatgacggg ggcgcggaaa ccgtaaatgc tggatgcgac
     4561 tgttgtgata aaaacgattt tcataaggcg gacaccttga attataggat tggaaatgcg
     4621 gtctgcattg atgaaatgcc ggatacgggg cgcggcgggc aaacctttgc caagcgcacc
     4681 gcatccgacg cttcgcccgc ccgggcaggg gcggcttcag acggcatttt atcctgaaat
     4741 ccgctgcata acgcccggcg atatacagta tcgcgttttg tgatgatttt acacccgaag
     4801 ggaaaacgga cttatttgaa tggcggttta atcgcggcgg ttgtttgcgg tttgataagg
     4861 ctgatttgcg tgtcgtgcag tccggaaacg ggcggggtgt ttggaaaatg ccgtctgagg
     4921 gttttcagac ggcattcttt catgcggcgg ttcggggttc gcggatgggg aggttgagga
     4981 gggcggcggt tcccgccagc agtgcgtcgg cgtaccacat ccagccgtag tcgccgaatt
     5041 gtgtaatcac gatgccgccg atgtacgagc cgaggaatcc gccgatttgg tggctgagca
     5101 tcgtcagtcc gaacagggtg gcgaggtagc gtgtgccgaa gagtttgccg gtaatggagg
     5161 cggtcggcgt gacggtggcg agccaggtaa atccgagtgc ggcggcgaaa atgtagaagt
     5221 tgaggtcggt cttgggcgaa aaaatgtaga tgagtatcat cgcggcgcgc gaggcgtaca
     5281 gtccgaacag gacgtatttg ccccgaacgc ggcttgtgca ccagccggaa aaaatgcagc
     5341 cggcgatgtt tgccagtccg atgatggcga tggatgtcga ggcgacggtg gcgggcagtc
     5401 cgcacagggc gacttccgtg ggtaggtggg ttacgagaaa ggcgatgtgg aagccgcagg
     5461 caaaaaagct taggtgcagc aggatgtagc ttggggtttt gaaggcggtt ttgactgctt
     5521 ctccgaggct ttgtccgtgt gtggcttggg cgtgttgggt gtgggcggcg ttgttgccgc
     5581 cggcaagcca ccatgagacg ggcagaatca gcagggcgat tgcgccccaa acgtaaaatg
     5641 tgcccgtcca gccgacttcg ggcaggacga cgagtccttg aaccagcggt gcgaacagga
     5701 attgtcctgc cgaaccgccg gcgttgacca gtccggatgc caagccgcgt ttgtgtgcgg
     5761 ggacttgggc ggcgacctgc cccataatga tggagaaacc gcctgagcct gtgccgaatg
     5821 cgagcagcag cccgacggca atcatcagcc cccaataagt cgggatgttg ggggcaatca
     5881 ggcaggcgca gacgagcagg actgcgccgc cgcttaatac cctgaacgcg ccgaaacggt
     5941 cggcaagcgc gccggacaat ggttgcgata tgccccacat cagttggaaa acggcgatga
     6001 tgaggctgaa ttgggcaatg ctcaattcgg tggtgttgac gacgggttgg acgaacagtc
     6061 cgagcgtcat cctcatgccg atggtaatca gcaggatgaa tgcggcggcg gtgatggtca
     6121 gccaaaattt gggtgttttc gatgcggtgt gcgtcataat gtgtattttg agtcggttaa
     6181 gtttgcggat tttatagtgg attaacaaaa accagtacgg cgttgcctcg ccttagctca
     6241 aagagaacga ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac
     6301 tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactataaca aaccggatga
     6361 ttcgggctaa ataattcctg attctttgga tatagccaac ggtatgcctg ctgatgccgt
     6421 cagaaagggc ttgatgggcg tttgcggttc gggttaatat ttgtgctgta tctgttaatt
     6481 ccggttagta aaatggggcg gggacgatga gtggccgtcg gtcggttttg cacgggcggt
     6541 aataatgtcc ggcgcaaggt gcgccgtttc gggaggggtg tgtgatgggt tttctgattg
     6601 gtttgagccg caacctgacg cggtttacgg ctttggtcat tttgcttgcg tcggcggttg
     6661 cgtttatcgg accggcttcg tttgcgtggg ttaagggcga tgttcaggtc ttggtgctgg
     6721 gcatcattat gctgggtatg gggatgactt tgggcaggga ggactaccgg attttggcgc
     6781 agcgtccttt ggatgtgttt atcggtgcgg cggcgcaata taccgtgatg ccgttgctcg
     6841 ccatcggtat tgccaagctg ttcgaccttt cgccggggct gactttgggg ctggtgttgg
     6901 tggggacgtg tccgggcggc gtgtcgtcca acattatgag tttccttgcc aaaggcgatg
     6961 tggcgttttc agtcggtatg acgacggtgt ctacggtgct ggctccggcc gttacgcctt
     7021 tgtggatgac ctgcctggtc ggtcagacgg tggatatgga cggttggggg atgttccggt
     7081 ttatgctgct ggtcacgctt ttgccggtgg tgctgggttc ggcggcaaat atcctgctgc
     7141 acggaaaaca ttggtttgaa aatgtccgtg ccgttatgcc gggcgtggcg gtattggcat
     7201 ttgcctgcat tgtcggcggt gtggcttcgg ttcacggaca ccgttttgcc gaatcggcgt
     7261 tggtaatggt gttggcgatt gcggtacaca atatcgccgg ctatgtgtca ggctattacg
     7321 ccgccgcgct gtttggtatg gatacggcaa aaaaacgcac cttggcaatc gaagtcggcg
     7381 tgcagaatgc cggtttggca acaggattga gcgcgaaatt tttcccaggc aaccccgaat
     7441 cggcggttgc ggcggcggtc gcctgcgtgt ggcattcggt ttcggggacg gtattgggca
     7501 acttgttcgc tctgtgggac aagcgtcggg acgcttagtc gggcgtgtcc gaaccgtccc
     7561 acgatatata gtggattaac tttaaaccgg tacggcgttg ccccgcctta gctcaaagag
     7621 aacgattctc taaggtgctg aagcaccaag tgaatcggtt ccgtactatt tgtactgtct
     7681 gcggctcgcc gccttgtcct gatttttgtt aatcactata aaaatgccgt ctgaaacggt
     7741 ttcagacggc atttcgatgt cggcggcggc tttgcggaat cagcctttga agcgtttgaa
     7801 gaccagcgtg ccgttggtgc cgccgaagcc gaaggagttg gaaatggcaa cgtcgatttc
     7861 cgcgtcgcgc gcttcgttgg cgcagtagtc caaatcgcag ccggcttcaa cgtcttgttc
     7921 aaaaatgttg atggtcggcg ggattttgcc gtcgtgtatc gccaaaatgc tgtacacggc
     7981 ctccacgccg cccgccgcgc cgagcaggtg gccggtcatg gatttggtcg agctgacgac
     8041 ggttttgtag gcgtgttcgc cgaacgcgcg tttgagggct ttggtttcgt tggcatcgcc
     8101 caagggggtg gacgtgccgt gcgcgttgac gtaatccacg tcttcgggat tgatgccggc
     8161 atctttcagc gcgcgggtaa cggcaagggc ggggccttct tcgttcggcg cggtgatatg
     8221 gtaagcatcg gaactcatgc cgaagccgac gatttcggcg tagattttcg cgccgcgttt
     8281 tttggcgtgt tccaattctt ccaacaccaa tatgcccgcg ccttcgccga taacgaagcc
     8341 gtcgcggcct ttgtcccacg gacgggaagc ggtggcgggg tcgtcgttgc gggtggagag
     8401 ggctttcatc gcggcaaaac cgcccacgcc caaagtgctg attgcgcctt ccgcgccgcc
     8461 ggcaaccatt atgtccgcgt cgccgtattt gatcagtcgg gcggaattgc cgatggcgtg
     8521 cgcgccggtg gtacaggcgg aaaccatccc gtagctcggg ccgcggtagc ctttgaggat
     8581 ggtaacgtgt ccggaaatca ggttgatcag cgaaccgggg ataaagaaag ggttgatttt
     8641 gcgcgcgccg ccttcgatta cggctttgcc ggtgacctcg atgctgggca gtccgccgat
     8701 gccggaaccg atgttcacgc cgatgcggtc tttgtcgagg ttttccacat cgtccaagcc
     8761 cgaatcggcg attgcctgca atgcggcggc aatgccgtag tggatgaata cgtccatccg
     8821 gcgcgcttct ttcgcgctga tgtattgtcc gatgtcgaaa ccgcgcactt cgccggcgac
     8881 acggctgttg atgtcggatg cgtcaaagcg ggtaatcgcg ccgatgccgc ttttgccggc
     8941 gagcagggtg tcccaagcct ctgcgacagt gttgccgaca ggggaaacct gacctaagcc
     9001 tgtaatgact actcttctct gactcatgat aacctcgctg ttggttgtcg gaatgggggc
     9061 atatgcggct gtcgtgcaga tgccgtctgt aattttcggc aggggttcaa acagtttgcc
     9121 atataaggga aaagcctcta ttgcgcggtg cagcagaggc tgttgtgtcg ggcgacgacc
     9181 ggttagccgt tgtgggcatt gatgtagtcg atagccagtt ggacggtggt gattttttcg
     9241 gcatcttcgt cggggatttc gcagccgaat gcttcttcca aagccataac cagctccacg
     9301 gtgtccaaag aatccgcgcc caagtcgtct tggaaagaag attcgttttt cacgtcggct
     9361 tcgtttacgc ccagttgttc agcaacaatt tttttaactt gttgttcgat gtttgacata
     9421 tcagtcgttc ctttatgcct tgcggcaggt tgtttaaggg aaataaatcg gtggtattgt
     9481 accgactttt aatagagttt tctatctaat gactattata tcaatatttg ccgatttgta
     9541 catttttggg tgcggcgggt tttgtcgttc aagtttgacc tgtgtgccgt atgtttgacg
     9601 ggatttcggt taaaatgacg gcatttccat ctgaagcaga aagccctgtc atgtatccac
     9661 ttgcccgccg tatcctgttt gcactcgatg ccgagaaagc ccaccacttc acgctcgacg
     9721 cgctcaacat ggtttataaa ttgggtctga ttcccgtaac cggcaaccgt accaaacctg
     9781 taaaactgat gggtttggat ttgcccaacc ctgtcgggct tgccgccgga ctcgataaaa
     9841 acggcgaata tatagacgca ttgggcgcgc tcggcttcgg tttcatcgaa atcggcacgg
     9901 taacgcccaa cccgcagccc ggcaacccgc agccgcgcct cttccgcgtt cccgaatatc
     9961 aaggcatcat caaccgcatg ggcttcaaca accacggcat cgatgccatg attcaaaaca
    10021 tcgaaaaaag taaattcagc ggcgtattgg gcatcaacat cggcaaaaac gctgtaacac
    10081 cgattgaaaa cgctgccgat gattatttaa tctgcctcga aaaagcctac gcacacgcaa
    10141 gttacattac cgtcaatatt tcctcgccca acactaaaaa cctccgcgcg ctgcaaggtg
    10201 gcgacgagtt gagcgcattg cttgaggctt tgaaaaacaa acaggcacag cttgcctctg
    10261 tacacgggaa atacgtcccg ctcgccgtca aaatcgcccc cgatttggat gaagcacaaa
    10321 ttgaagacat cgcccacgtt gttaaatccg tcgaaatgga cggcatcatc gctaccaata
    10381 ccaccatcga caaatcaagt ctcggcagcc atccgctcgc aggcgagcag ggcggtttga
    10441 gcgggctgcc cgttcatgaa aaaagtaatc gggtgttgaa gctgttggca gaccacatcg
    10501 acggcaagct gccgattatc ggcgtaggcg gcattatgga aggcggggac gcggcagaga
    10561 aaatccgctt gggcgcgacc gccgtccaag tgtacagcgg attgatatac aaaggtccgg
    10621 cattggtcaa agaatgtttg aaggctttgg cgcgatgacg cgtcccgccc aaaatgccgt
    10681 ctaaacgcac gttttgccgt tcagacggca ttttcatttc ctttttccgc ctgccgcccc
    10741 ttgaaaatcc cttacgcgcc gccctgtttg aaacaaagca aacgcccgcc gcgaaccgga
    10801 caatcttgat acgcagccaa agcaagtccg gcggtaagtt gttaaatata taagcaattg
    10861 atacaaaaaa taattttata ctgtggcgga cggcttggga aaacgcaaaa tatgaacggc
    10921 gttgaaattc atgatgtgga aaataaggat acggcggata atcaaaagat tttatattta
    10981 aggatttgat atgtttaagg acgaactaaa tgaatttatc cgattgatat ccgatccgga
    11041 aagtgaattg gatgaatggt atttgagtga ttttaaagat gaacatatat gggaaatgca
    11101 aagttacgaa gcattttcat gtttgcggga agcagtcccc tatttgtttg cttatccccg
    11161 atacgggtat gaactgttgg agataatttc cgcgttaaaa gaaacatctg atacgacaga
    11221 attattttat gagccgggta tcgtgccttt gctgattgat ttatataaag aagatagtta
    11281 tctggtaaat atggtcaaac ggatattcaa atgatgaaag gggcgcgggc gtttgcttgg
    11341 cggaaggcag gtaactcaat ttgaaggacg cggaagccga tacgttaaaa aggatgaaaa
    11401 atggaaaacg ggcaacggaa taaccggttt cctttggaaa aacgaatttt ttatctggag
    11461 cattcggggc ggtatctgat gatttgcgcc ttatcggatt attcccaaaa caaacatact
    11521 gtcgttatgg caaatttcat ctatccggat gaaaaaacgg attagcggaa tttggatgat
    11581 ttattcaatg agttggtttt agaggaattg caggcttcat ttatggattg gcatccgact
    11641 gttgaagagg caatcagccg tcatttggaa gacttttcgt aacaggcgtc aatcggaagg
    11701 ggcggaatga cggggcaggc aatcggagta aaaaatgaac cttgatttaa ccgcgcaaaa
    11761 agtccgtctt tcttggaagg atattctgtg ggggtatggg aataaatact tgggttgggc
    11821 tgatgtggca gcttatgccc gaaaaatgac gctttcagat catgatgaac gtgtgttcaa
    11881 actatcttta atcaacaaat ccaatattct tgaattaaag cctgttctgg aagatttggc
    11941 ttcggaaatg agggattatt cccctaaaaa ttggctgtac gtcctcttaa gcgatgtatt
    12001 ccatagaaaa gaagaatttg aggatccttt gggggaagtt gaaaaaattt atgcagattt
    12061 tgattatccg gaagaaatag aatcatttgt caggtatatg ccgcccaaag acggttatat
    12121 tccttctgcc cacacctatg aagaaaatat tgcccggtta tattctcact gggaacacta
    12181 tttgaacaac ggcggagggc agggttaaaa ccggcaatcc gatgccgtct gaagcattat
    12241 ccggccttca gacggcaaac ggattagagc ggaagatttg aaagcggggg cgggctgcat
    12301 tccggaagcg gcaaaacccg aaccgcccgc atcgttgccg ataaaccgat aaggaaatcg
    12361 ttttgccgga ccgggtgcaa atgtttgata acaatcctgc cgcccggcaa aaatgcttta
    12421 aagaaagcag gattggttaa tttaaaagga aaaattaaaa aatgacggtt gataaggaac
    12481 aagtagaaaa gatgatttat tctgaaaata aacagagtgt tattgacggt atgctcggta
    12541 tgaccttcag ctcggaggag gatgaaatcc cctggatttc cgagaagttg acggagttgt
    12601 cgaaacataa agacttggat attgccagat tgtcattaac ctgtttcgga catctcgcca
    12661 ggatgcatga aaatatcggc gattgcgata aagtgattgc tttattgctc tcaaaacaag
    12721 gtgatccgga ttttcaaggt tttgccgaag atgcgttaga cgagatttct ttatttatat
    12781 ttaaaaagcg tccgtaagcg cggattaaac ctgtcaatcc gatgccgtct gaagcgttat
    12841 ccggctttca gacggcatcg aaggttgcta tgatatagtg gcttgacttt aaaccggtac
    12901 ggctccgcct tgccctgatt tgaagtgaat ctgccatcat cttcccgtca tccttccaaa
    12961 cggaatccga aatgtccgac aaccgcctcg acaccgcccg gcaccattcc ctgttccttg
    13021 cccgccagct cgacaacggc aaactcaagc ccgaaatttt cctgcctatg ctggacaagg
    13081 tgttgaccga agctgatttc caagcttttg ctgattggga caaaatccgc gcggaagaaa
    13141 acgaggaaga attggcgcgg cagttgcgcg ggttgcgccg ttatgtggtg tcgcagatta
    13201 tcgtgcgcga tatcaaccgc atcagcgatt tgaacgaagt aacccgcacg attacgctgt
    13261 ttgccgattt tgccgtcaat accgcgctgg attttgccta cgcctattat cgggacatgt
    13321 acggcacgcc gatcgggcgt tataccaaat cgccgcagca tttgagcgtg gtggcgatgg
    13381 gcaaggcggg cggctatgag ttgaacgtgt cttccgacat cgatttgatt ttcacctatc
    13441 ccgaatcggg cgacaccgac ggcaggcgcg aacgggggaa tcaggagttt tttaccaaag
    13501 tcgggcagaa actgattgcg ctgttgaacg acatcaccgc cgacggacag gtgttccgcg
    13561 tcgatatgcg gctgcggccg gacggtgatt cgggcgcgtt ggtattgagc gaaaccgcgc
    13621 tggagcaata tttgattacg caggggcgcg aatgggaacg ctacgcgtgg tgcaaaggcc
    13681 gcgtggttac gccgtatccg aacggaatca aatcgctggt gcgccctttc gtgttccgca
    13741 aatatttgga ttacagcgcg tatgaggcga tgcgtaagct gcaccgccaa atcagcagcg
    13801 aagtcagcaa aaaaggcatg gcggacaaca tcaaactcgg cgcgggcggc atccgcgaag
    13861 ttgaatttat cgcccagatt ttccagatga tacgcggcgg ccaaatgcgt gcgctgcaac
    13921 tgaaaggcac gcaggaaacg ctgaagaaac ttgccgaaac cgggatcatg ccgtctgaaa
    13981 acgtcgaaac cctgcttgcc gcctaccgat tcctgcgcga tgtcgaacac cgcctgcaat
    14041 actgggatga ccagcaaacc caaaccctgc cggcttcgcc cgaacagcag caactgcttg
    14101 ccgaaagtat gggtttcgac agctacgccg ctttttcaga cgacctcaat gcccaccgca
    14161 ccaaagtcaa tcagttgttc aacgaaattt tgagcgaacc cgaagagcaa accctagaca
    14221 ccagcgaatg gcaatgggca tggcaggaaa agcccgacga agaagagcgg caaggtcgtc
    14281 tgaaagaaca cgggttcgat gccgaaacca tcgccgcaag gctcgaccaa atccgaaacg
    14341 gtcataaata ccgccatctc tccgcacatg cccagctgcg tttcgatgcg gttgtgccgc
    14401 tgttcgtaca ggcggcggca gagcaaagca acccgaccga tacattgatg cggctgttgg
    14461 attttctcga aaacatcagc cgccgatccg cctatctcgc cttcctcaac gaacatccgc
    14521 aaaccttggc gcaactggcg cagattatgg gccaaagttc ttgggtggcg gcgtatctga
    14581 acaaatatcc gattttgttg gacgaactca tcagcgcgca gcttttggat accgcgtttg
    14641 attggcaggc actcgccgcc gccctttcag acggcatcga agcctgcggc ggcgatactg
    14701 aagcgcaaat ggacgccctg cgccgcttcc agcacgccca agtcttccgt ctcgccgtcc
    14761 aagaccttgc cggactgtgg acggtagaac ccctctccga ccaactctcc gccctcgccg
    14821 acaccatact cgccgccgcc ctgccgtgcg catgggcgga catgccaaaa aaacaccgcg
    14881 acacaccgca attcgccatc gtcggctacg gcaaactcgg cggcaaagaa ctcggctacg
    14941 cctccgacct cgacttggtc tatctttatg acgacccgca tcccgatgca ggcgacgtgt
    15001 acagccgcct tgcccgccgc ctgactaact ggctttccac cgccaccggc gcaggcagcc
    15061 tctacgaaac cgacctgcgc ctgcgcccca atggcgatgc aggtttcctc gcccacagca
    15121 tcgccgcctt tgaaaaatac cagcgcgaaa acgcatggac gtgggaacac caatccctta
    15181 cccgcgcccg ttttatctgc ggtacgcccg aaattcagac ggccttcgac cgcatccgta
    15241 ccgaaatgct gaccgccgaa cgcgaccaaa cggttttagc aggcgaaatc atcgaaatgc
    15301 gcgaaagaat gttccccacc catccgccgg ttgacagcaa cgtcaaatac gcgcacggcg
    15361 gcgtagtaga cgtcgaattt atcgtccaat atctgatact tgcccacgcc cgaaaatatc
    15421 cgcaactcct cgacaactac ggcaacatcg ccctcttgaa catcgccgcc gactgcggtt
    15481 tgattgataa aacgcttgcc ggacaaagcc gcaccgccta ccgcttctac cgccggcagc
    15541 agcacaacac caaactgcgc gacgcggcaa aaaccgaagt aaccggcgaa ctgttggcac
    15601 attacggcaa tgtcaggaaa ttgtggcggg aagtgttcgg cgaagaagcg gcaaccgcct
    15661 gaacaaaaaa tgccgtctga agcctgacaa tctgggtttc agacggtatt ttcgtaccgt
    15721 gccgttttaa ggttgcggca gagctaaagc gatttatcgg gaatggctga aacccaaaaa
    15781 ccggattcct ctttcgcggg aatgacggga tttcaggctt ctgtttttgt gggaatgatg
    15841 ggatttttta tccaagcaaa aatcaaaaca aacaaataag a
//
LOCUS       DAWFJR010000041        15647 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWFJR010000041 DAWFJR010000000
VERSION     DAWFJR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15647
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.041"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            86..1333
                     /locus_tag="WGR04_001182"
     CDS             86..1333
                     /locus_tag="WGR04_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0794739.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYIPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKAAAVK
                     SRPAVPAAAQPPVQSAPVDINAATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNML
                     SIGQIVKVKPAGYAAPKTSAVESRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAENKA
                     VPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGVDIAGNAGQPVLAAAD
                     GKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDAS
                     RTQLHFEVRQNGKPVNPNSYIAF"
     gene            complement(1540..2022)
                     /locus_tag="WGR04_001183"
     CDS             complement(1540..2022)
                     /locus_tag="WGR04_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0794740.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(2175..2336)
                     /locus_tag="WGR04_001184"
     CDS             complement(2175..2336)
                     /locus_tag="WGR04_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794741.1"
                     /translation="MSGQPEKNHNPLLEDERKNPVYRMGQAVAGFMLVVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(2410..2871)
                     /gene="recX"
                     /locus_tag="WGR04_001185"
     CDS             complement(2410..2871)
                     /gene="recX"
                     /locus_tag="WGR04_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ0794742.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPYAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(2944..3654)
                     /locus_tag="WGR04_001186"
     CDS             complement(2944..3654)
                     /locus_tag="WGR04_001186"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ0794743.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLSNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETA
                     TGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKDEE"
     gene            4061..5329
                     /locus_tag="WGR04_001187"
     CDS             4061..5329
                     /locus_tag="WGR04_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ0794744.1"
                     /translation="MSSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRV
                     LEVNFPVKLDDGTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCC
                     VAGIPYGGGKGGITLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIM
                     SWMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGA
                     TYAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITN
                     EAGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGPTTPEADVI
                     LRQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNL
                     AQEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(5387..6193)
                     /locus_tag="WGR04_001188"
     CDS             complement(5387..6193)
                     /locus_tag="WGR04_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ0794745.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            6538..6747
                     /locus_tag="WGR04_001189"
     CDS             6538..6747
                     /locus_tag="WGR04_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ0794746.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            6836..8050
                     /locus_tag="WGR04_001190"
     CDS             6836..8050
                     /locus_tag="WGR04_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ0794747.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(8137..10710)
                     /gene="clpB"
                     /locus_tag="WGR04_001191"
     CDS             complement(8137..10710)
                     /gene="clpB"
                     /locus_tag="WGR04_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ0794748.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRIKMELDSKPEQMDKLDRRIIQLKMEKMHVAKETDDASKKRLELIDEEIDG
                     LQKEYADLDEIWKAEKAASSSTADIKKQMDDIKVKIEQAKRQGDFARASELEYGELPK
                     LGAQLQAAESNPDGKKQNKLFRTEVGADEVAEIVSRMTGIPVSKMMEGERDKLLKMEE
                     VLHRRVVGQDEAVRAVSDTIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAGFL
                     FDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDEVE
                     KAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAVKE
                     VVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTVSD
                     AALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRLKF
                     A"
     gene            10971..11981
                     /gene="trpS"
                     /locus_tag="WGR04_001192"
     CDS             10971..11981
                     /gene="trpS"
                     /locus_tag="WGR04_001192"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ0794749.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFQELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            11987..12328
                     /locus_tag="WGR04_001193"
     CDS             11987..12328
                     /locus_tag="WGR04_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794750.1"
                     /translation="MPSEPLKGLPEQNVVRLTGKHPGDLEAVSGKCMETDGKGAPSGW
                     AANGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            12649..12972
                     /locus_tag="WGR04_001194"
     CDS             12649..12972
                     /locus_tag="WGR04_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794751.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKM
                     KDAAK"
     gene            complement(13181..14689)
                     /gene="ppx"
                     /locus_tag="WGR04_001195"
     CDS             complement(13181..14689)
                     /gene="ppx"
                     /locus_tag="WGR04_001195"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ0794752.1"
                     /translation="MTTHPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKIIAKDFQAAISAARNEIQRISKNMRREGWDSAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(14805..15537)
                     /locus_tag="WGR04_001196"
                     /pseudo
     CDS             complement(14805..15537)
                     /locus_tag="WGR04_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
BASE COUNT         3501 a         3948 c         4303 g         3895 t
ORIGIN      
        1 gtcgggcttt gcgcttgaga agaacgcgca tttttattat gatataccgc attcaggcta
       61 tatatcggaa catgaaggtt atcttatgtt gaaacaaacg acacttttgg cggcttgcgc
      121 ctcctttgcc gcactgttgg gcggttgtgc tacccagcag cctgcccccg tcattgcagg
      181 caattcaggt atgcaggacg cgccgtcttc ggcagtttac aacaacccct atggagcaac
      241 gccgtacagc ccggctcctg ccggcgatgc gccttatgtg ccgccggtgc aaagcgcgcc
      301 ggtttatacg cctcctgctt atgttccgcc gtctgcacct gccgtttcgg gtacatacat
      361 tccttcttac gcacccgtcg acatcaacgc ggcgacgcat actattgtgc gcggcgacac
      421 ggtgtacaac atttccaaac gctaccatat ctctcaagac gatttccgtg cgtggaacgg
      481 catgaccgac aatacgttga gcatcggtca gattgttaaa gtcaaaccgg caggatatgc
      541 cgcaccgaaa gccgcagccg taaaaagcag gcccgccgta ccggctgccg cgcaaccgcc
      601 cgtacagtcc gcacccgtcg acatcaacgc ggcgacgcat actattgtgc gcggcgacac
      661 ggtgtacaac atttccaaac gctaccatat ctctcaagac gatttccgtg cgtggaacgg
      721 catgaccgac aatatgttga gcatcggtca gattgttaaa gtcaaaccgg caggatatgc
      781 cgcaccgaaa acctcagccg tagaaagcag gcccgccgta ccggctgccg tgcaaacccc
      841 tgtgaaaccc gccgcgcaac cgcctgtgca gtccgcgccg caacctgccg cgcccgctgc
      901 ggaaaataaa gcggttcccg cgcccgcccc gcaatctcct gccgcttcgc cttccggcac
      961 gcgttcggtc ggcggcattg tttggcagcg tccgacgcaa ggtaaagtgg ttgccgattt
     1021 cggcggcaac aacaagggtg tcgatattgc cggtaatgcg ggacagcccg ttttggcggc
     1081 ggctgacggc aaagtggttt atgcaggttc cggtttgagg ggatacggca atctggtcat
     1141 catccagcat aattcttcct tcctgaccgc atacgggcac aaccaaaaat tgctggtcgg
     1201 cgaaggccag caggtcaaac gcgggcagca ggtcgctttg atgggcaata ccgacgcttc
     1261 cagaacgcag cttcatttcg aggtgcggca aaacggcaaa ccggttaatc cgaacagcta
     1321 tatcgcgttc tgactttctg cctgaaatca agttggaaat gccgtctgaa aggttttcag
     1381 acggcatttt tgtttttggc ggctgtgttt tcaaatcatt cggaagggac ggggcatata
     1441 aatttgtttg actgtgtttg ggtatttgct tgatttcatc cgtttgcagt agaaacggat
     1501 ggcgcgatgt gcctgtcttt cagacggcat agtccgccgt cagcagccgc aggacatgtc
     1561 ttcagaggct tgcaggctga tgtctctgcg ttttttggct ttttcgtagc ggcagcgttg
     1621 gcggtcggtc aggatttcca gcggagggac agggacgggt ttgccgtctt tgaccgcaac
     1681 catcgtgaag tagcagctgt tggtatggcg gatttcgccg gtgcggatgt tttgtgcttc
     1741 gacacggatg ccgatttcca tcgaggtgcg tcccgtgtag ttgacagcgg cgtaaaaggt
     1801 aaccaaatcg ccgatgtgga tgggttcttt aaacaggact ttgtcaaccg acagggtaac
     1861 gcaataattg ccgctgtaac ggctggcgca ggaataggcg acttggtcga gcaggagcag
     1921 gagttcgccg ccgtgtacgt tgccgctgaa attggcggtg tccggcatca tcagttcgga
     1981 cataatgagt tcgtgggaag gcagttggcg ttgttgtgtc atagtgtacc tgttttaaga
     2041 aagggtgttt tgaaatgggt gtgggaaaat tttagggaat ggggaagtgg aattcaagct
     2101 aaaaaaagct atggcgggat agtctgcaga aatgggccgc cgatgccgtc tgaagccttc
     2161 agacggcatt ttgtttagga aagccaaaaa cggaagacca ggaaaaacac gagtgccaat
     2221 acgcctgccc aaacgacgag catgaatccg gcaactgcct gccccatgcg gtacacgggg
     2281 ttcttgcgtt cgtcctcaag gagggggtta tgattttttt cgggttgtcc ggacattttg
     2341 ttctcaatcg gaaaagttgg aaacgggcgt aaggttatgt tgccgtgcaa aaagattcaa
     2401 gaaggaaggt cagcagccgt cttcccaatt ttcgtcccaa gcgtgtttca atgccgtctg
     2461 aacggtatcg gcatcgaatc cgcgataggc gaggaaacgc gcctgttttt gtttttcttt
     2521 gaggtcggcg gcggggtgtt tgaatttttt acgcaacacg gctatggcgg cctgtttttc
     2581 gcttgagcgg tcgggaagca ggttgcggct ggtttcttca tctatgccct gttgcgccaa
     2641 agcctgtttc agcctcaatg aaccgtgttt gcggcttttg ctgcggatat aggcttcggc
     2701 atagcgcaaa tccgactgcc agttgcgttc ggcaaattcg tttaacacgt tttccaactc
     2761 ctcttcgctt tcggcgtacg gtgcaagttt gcgtttcaga ccgatgcggc tgacttcttg
     2821 gcgcgagagg atgtccatcg cgcgggcgcg tagggatttt tgcggtttca tggcggcggt
     2881 ttcggcacgg aaagattcag acggcatggg aaacggattc cgatgccgtc tgaacagatt
     2941 gttttattct tcgtctttat ttttctgcgg ttgcaggttt tcgtaaattt cgggcagcga
     3001 gccgataatc cagtcggggc gggtcgcatc gtcttgcgag agcagcgtca tatcgccgta
     3061 accgaaggta acgccgacgc tcaggcagcc ggcggctttg gcggcgatga tgtcgttgcg
     3121 cgagtcgccg accataacca tgtttgcaac atcgataccc aaaacttcgg cggcgtgccg
     3181 cagcggcagg gggctgggtt ttttctcggg caggctgtcg ccgccgagta tcaggctgaa
     3241 gtagtcggca agtccgagct gtttcaacag ttcgacggca aggatttcgt ttttattggt
     3301 aatgactgcc agcgggatgc ccaaagattt gagcaatgcc agcccggttg cggtttcggg
     3361 ataggggcgg gtgaagacgc tcaaatggtc gcggtagtat ttcatatagg atacgaaacc
     3421 tttttcccac agttcggaat cggcttcgcg gtcgcggtcg ttggagagga cgcggtgaac
     3481 cagtttgccg atgccgtcgc ccacatagct ttcgaccact ttggcgggca gcggtttcat
     3541 accgagttgt tccaacatcg cttctgcggc ggcggcaagg tcggggacgg aatcgcacag
     3601 tgtgccgtcc aaatcgaagg cgacggcttg gacgtgttcg atggctgcat tcataatctg
     3661 atttcctgtc gggttgaaag ggtttatttt aacatcgttt gcacagggct tctaatgtat
     3721 agattgttaa caatcgcaac aggttttgca ggcggtgttt tcggatttcg gggcggaatc
     3781 gggcaagggt ttgaaatccc agcgatgttc caattaaatt tttaaaaaat aaaaggatgc
     3841 ctatatcttt atttaatttg acatttattt ttcggacgcg cgcgctgctt tttcaggcgg
     3901 agtccgggct gattccgtgc ggcttgaaaa ataaaatcat atatagatta atcaaatcgg
     3961 gcttatgccg actacacgct attacatttt gtcgggaaat tggttatagt aatgagcgaa
     4021 atgcacgccc ctgaaaatct ttttgtgaaa aggaagcaaa atgtcgtctg aagcccttgc
     4081 aaaagaaacc ctcaatccgt ttgaaatcgc gcgcaaacag gtcaaaaccg cctgcgaccg
     4141 gctgaaaacc gatccggccg tttatgaaat cctgaagagt ccgacccgcg tgttggaagt
     4201 caatttcccc gtcaaactcg acgacggcac ggtcaaaacc ttcacgggct accgttcgca
     4261 acacaacaac gccgtcggcc cctataaagg cggcgtgcgc ttccatcccg gagtgaatct
     4321 ggacgaagtc aaagccctgt cgatttggat gaccatcaaa tgctgcgttg cgggtattcc
     4381 ttacggcggc ggcaaaggcg gtattacctt ggatccgcgc gattattccg aggcggaact
     4441 ggaacgcatc gctcgcgctt attccgaagc catttctccg ctgattggcg agaaaatcga
     4501 tattcccgcc cccgatgtga ataccaacgg caaaatcatg tcttggatgg tggatgctta
     4561 tgaaaacgtg gtgaaacatt ccgcgccggg cgtatttacc ggaaaaccgg ttgagttcgg
     4621 cggctctttg gcgcgtaccg aggctaccgg ttacggcgtg aacctcgccg ccgtccaagc
     4681 tttggaaaaa ctgggcaagg acgtgaaggg tgccacttac gccattcaag gtttcggtaa
     4741 tgtgggttac cacacaggtt attacgcgca tcaatccggc gcgaaagttg ttgcggtttc
     4801 tactgtcgat gttgccattt acaacgaaaa cggcttggat atggaagctt tgtttaaaga
     4861 gttccaagaa aaaggcttca tcaccaacga agccggttac ggcaaagaaa ttaccaacgc
     4921 cgaacttttg gctttggatg tggacgtact cgccccttgc gcgctggaaa accaactgac
     4981 ttccgaaaac gccggtaaag tccgcgcgaa aatcgtggtc gaaggcgcga acggcccgac
     5041 tacgcccgaa gccgacgtta tcctgcgtca aaacggcgta ttggtcgtgc ctgatatttt
     5101 ggcgaactgc ggcggcgtgg tggtatctta ttttgaatgg gtacaaaacc tgcaaggcta
     5161 ttattgggag tttgacgaag ttcaagagaa agaaaccgtc gtcctgcgac gcgcgttccg
     5221 cgatatatgg aacttggctc aagaatacga tgtcgatttg cgaaccgcgt cttacatgat
     5281 gagtatccgc cgtgttgaaa aagcgatgaa gctgcgcggt tggtattgat gtttgaacaa
     5341 aaaatgccgt ctgaagcctt cagacggcat ttttgtgcgg gcgggattaa tggtgcgaat
     5401 ggccgatttg gaaggttaaa gtcgagtagt tcgcctgttt ttggcacacg ctttgatcgg
     5461 ggaagtcggc tttgtgttcg acattggctt tccagaagcc ttggcgcaag gggatgatgt
     5521 ccacttcgcc tttgtcgtct gtgctgtcgg agaaagcctg tgcttcggtt ttgtgcgttt
     5581 tgctgcggtc gctggtgtcg aagccgtcaa aggtggcggt aacggtggca ttgggcagcg
     5641 gttcgccacg gaacagaacg cggactttga agcgttcgcc tacgtgaatg ttggcgggat
     5701 tgtccagcgg gacgatttcc aagttttgtc cgaccggttt ggtgatgatg gcggtgtccg
     5761 cgctttcgtg tccgacgttg acgatgtttt tgccgaacat tcgggtttgt tcgcaatagc
     5821 ttgcgtcagg catttctttg atgcccgcct gtttccagcc tgctttgttt tttgaccaga
     5881 aagtaggctg atattcggcg atgacgaggt aactgccgtc cttaacggga cggttgcttc
     5941 ggtactggta gttgtatgtg ccgcgttgaa tcatgttttc cttgcctttt tcggtaacca
     6001 gctgcatcgg tttgctgaag atgtgcaggc ggtctttggc gatgggttcg agttcgggaa
     6061 attcgccgta gcccaagtcg gctttaaggt attcgccgcc gtgcgtgtgg gcggtttcga
     6121 cccagacgcg gtgggcgtgg gcggatgtgg caaatagggc ggaaacggcg agcagtgtca
     6181 atgttttctt catggtgcag cctttgtctc gtgtgggtta aagattttgt tatgttataa
     6241 caaaataggt atcgtgtgaa gatggatttg ctgtctgaaa actgtctttt tgtatagtgg
     6301 attaacaaaa accagtacgg cgttgcctcg ccttagctca aagagaacga ttctctaagg
     6361 tactgaagca ccaagtgaat cggttccgta ctatctgtac tgtctgcggc ttcgtcgcct
     6421 tgtcctgatt tttgttaatc cactatatcg atttgaattt ttcagaaaat gaagcggacg
     6481 tttgggcgga ggcaacttgt ttgataagat agcaatattt caaaacggag aaagatcatg
     6541 ccttatgtca atattaaagt aaccggcggc aaggaagcac cgactgccgc tcaaaaagcg
     6601 gaactgatcg gcggcgtaac cgaattgctg gcacgcgtgc tgggcaaaaa tcccgaaaca
     6661 acggttgtcg tgattgatga agtggatacc gataactggg gaataggcgg caaaagcgtc
     6721 agcgaacggc gcaaagaggg caggtaaaaa gcctgaaaat ctcggtttga tgctttaaat
     6781 tccgcgtgaa aaagagtaca ttcccaccca ttgcccaaaa tttacggaac acattatgga
     6841 taaatttccc aagtctgcaa agctcgatca cgtctgttac gacatacgcg ggccggttca
     6901 caaaaaagcc cttcagttgg aagaggaggg caataaaatc cttaaactca atatcggcaa
     6961 ccctgcgccg ttcggctttg aagcccctga tgaaatcttg gtcgatgtca tccgcaacct
     7021 gccgacttcg caaggctatt gcgattctaa agggctgtat tccgcccgca aagccattgt
     7081 tcactactat cagaccaaag gtttgcgcga tattacggtt gatgatgtct atatcggcaa
     7141 cggcgtgtcc gagctgatta cgatgtctat gcaggcgttg ctcaacgacg gcgacgaaat
     7201 cctgattccc gcgcccgact accctttgtg gacggcggcg gcaacgcttg cgggcggtac
     7261 ggtacgccat tatctgtgcg acgaagaaaa cggctggttc cccaaccttg ccgatatgga
     7321 agccaaaatc acgcccaaaa ccaaagccat cgtcgtcatc aatcccaata atccgacagg
     7381 cgcggtgtac agcagggaaa tcctgttgga aatcgccgaa ctggcgcgca agcacggttt
     7441 gattattttc gccgacgaga tttacgataa aatcctttat gacggcgcgg ttcaccacca
     7501 catcgccgcg cttgcccccg accttttgac ggtaacgttc aacggtttgt ccaaatccta
     7561 ccgcgtcgcc ggcttccgcc aaggctggat ggtgctcaac gggccgaaac atcacgcaaa
     7621 aggttacatc gagggtttgg atatgctctc gtccatgcgc ttgtgtgcca atacgccgat
     7681 gcagcacgcg attcagacgg cattgggcgg ctatcagagc attaacgaat ttatcctgcc
     7741 gggcgggcgg cttttggaac agcgcaacag ggcgtgggaa ctggtcaacc agattcccgg
     7801 cgtatcctgc gtcaaaccga tgggcgcgat gtatatgttc ccaaaaatcg ataccgaaat
     7861 gtaccgtatc cgcgatgaca tgaaattcgt ttacgatttg ctggtgcgcg aaaaagtctt
     7921 gctggtgcag ggaacggggt ttaattggat caagcccgac catttccgca ttgttacgct
     7981 gccttacgtc catcagattg aagaggcgat gggcaggctg gcaagattcc tgcaaaccta
     8041 ccgccaataa ggggacggtt tgtctgccga ggataaaaaa tgccgtctga aacggagatt
     8101 cccgtttcag acggcatttt caacagcagg aacgaatcag gcaaatttca gtctgtcgcc
     8161 gtcggcttct accctgattt cgctttcggg cgcatagttt ccggcaagca gagcttttgc
     8221 cagagggttt tcgatttccg actggatggc gcgtttgagc ggacgtgcgc cgtaaatcgg
     8281 gtcgaaaccg gctttggcga tgatgtccag tgcggcatcg gaaacagtca ggcgcaggtt
     8341 ttgtttttcc aaacgttttt ccaaaccttt gagctggatt ttcgcaatgc tgcggatgtt
     8401 tgcctgatcc aaaccgtgga acacgaccac ttcgtcgatg cggttgatca tttcaggacg
     8461 gaaatgttct ttcacatcct ccatcacaac ttctttcacc gcttcgtagt cttgagtacc
     8521 catttgttgg atatgttggc taccaatatt ggaagtcatc acaatcacgg tgtttttaaa
     8581 atctaccgtc ctgccttgtc cgtcggtcag acggccgtca tccaatactt gcagcaggat
     8641 gttgaacacg tcgggatggg ctttttccac ttcgtccaac agaatcacgc tgtacggttt
     8701 gcggcgcact tgttcggtca ggtagccgcc ttcttcgtag ccgacatagc ccggaggcgc
     8761 gccgattaag cgggcaacgg cgtgtttttc catatattcg gacatatcga tgcggatcag
     8821 gtgatcctcg ctatcaaaca ggaagcctgc caatgcctta cacaattcgg ttttacccac
     8881 cccggtcggg cctaggaaca ggaagctgcc gtaaggtttg ttcggatcgg caaggccgga
     8941 acggctacgg cggatagtgt cggacacggc acgcacggct tcgtcctgac cgaccacgcg
     9001 gcggtgcaat acttcttcca ttttcaacag cttgtcgcgt tcgccttcca tcattttgga
     9061 tacgggaatg ccggtcatac gcgatacgat ttccgccacc tcgtccgcgc cgacttcggt
     9121 gcggaagagt ttgttttgtt ttttgccgtc ggggttgctt tccgctgcct gcaactgcgc
     9181 acccaatttc ggcaactcac cgtattcgag ttcggacgca cgggcaaaat cgccttggcg
     9241 tttggcttgt tcgattttga ctttgatgtc gtccatctgt tttttgatgt cggcggtgct
     9301 ggacgaggcg gctttttctg ctttccagat ttcgtccaaa tcggcgtatt ctttttgcaa
     9361 accgtcgatt tcttcgtcta tcagttccaa acgttttttg ctggcatcat cggtttcttt
     9421 ggcaacgtgc attttttcca ttttgagctg gataatgcga cggtcaagtt tatccatttg
     9481 ctcaggtttg ctgtccagct ccattttgat gcggctggca gcttcgtcaa tcaaatcaat
     9541 cgctttgtcg ggtaggaagc ggtcggtgat gtagcggtcg ctcaattccg cagcggcaac
     9601 gatggcgggg tcggtgatgt cgatgccgtg gtggatttca taacgctcct gcaaaccgcg
     9661 caggatggcg atggtgtctt ccacactggg ttcgccgacc aatacttttt ggaaacggcg
     9721 ttcgagtgcc gcatctttct cgatgtactg acggtattcg tccaaagtag tcgcaccgat
     9781 acagtgcagt tcgccccgcg ccaaggccgg tttcagcata ttgcccgcgt ccatcgcgcc
     9841 gtcggttttg cccgcgccga ccaaagtatg gatttcatca atgaaaatca gagtgttgcc
     9901 gtcgtctttc gccaaatcgt tcaacacgcc tttcaagcgt tcttcaaatt cgccgcggta
     9961 tttcgcgccg gcaatcaaag ccgccaaatc caaaaccagc aggcgtttgt tacgcaggga
    10021 ttcaggtact tcgccgttga cgatacgttg cgccaagcct tcaacaatgg cggttttacc
    10081 cacacccggc tcaccaatca gcacagggtt gtttttggta cggcgttgca atacctgaat
    10141 cgcgcggcgg atttcgtcgt cacgaccgat aacggggtcg agcttaccgt ctcgggcgcg
    10201 ttgggtcaga tcgagcgtat attttttcaa agcatcgcgt tggtcttcgg cattggcatc
    10261 gttcacgttt tgtcctcctc gtaccgcgtc aatcgcggca ttgatgtttt gttcggtcgc
    10321 gccggcttct ttcaaaattt tgccggccgc atcgttctgc tgcaccaagg caagcaggaa
    10381 aagttcactg gcgatatagg catcaccgcg tttggtggca gccttgtcca tcaggttcaa
    10441 caccgcctgc aattcgcggc tgggcagaat gtcgccgccc tggccggaca ctttcggcag
    10501 gctgttcaaa tgctgctgca aacgctgttt cacctgcggc acgttcacgc ccgcatgagc
    10561 caagagcgcg gcggctccgc tgttttggtc gtcaagcagg gcttttaaca caaagcccgc
    10621 ttccagatag ctgccgtccg cagccaacgc caaactctga gcttctgcaa gggcttgttg
    10681 gaatttggcg gttaatttgt cgtatcgcat tttcgtttcc ttttcaaaat gtccgctgtc
    10741 gaagcctata tgtgcataat tgtggataac tcaagttctg ttttctgttt ttctatattt
    10801 aattcgatat atcattgaat ttaaagtata taaaaatgta taataatgtg tataactata
    10861 tcttcttaat atggaaaagt ctgttgtcgg ctggatgtag gtggcaaatc gggtataatc
    10921 ggcgcatctt tttccctttc agacggcatt gatgccgcaa ggacattttt atgagcaaaa
    10981 aacgagttct gaccggcgta accaccaccg gcatcccgca tttgggcaac tacgtcggcg
    11041 ccatccgccc cgccgtccgc gcggcgcaaa accccgatac cgaatccttc ctcttcctcg
    11101 ccgactacca cggcatcatc aaatgccacg agccggagat gattcaccaa tccacccaag
    11161 ccgttgccgc cacttggctt gcctgcggac tcgaccccga gcgcaccacc ttctaccgcc
    11221 aaagcgacat tcccgaagtg atggaattga actggattct gacctgcatt accgccaagg
    11281 gtttgatgaa tcgcgcccac gcctacaaag ccgccgtgca ggcaaatgcg gaaaacgggc
    11341 aggaagaccc cgattttggc gtggaaatgg gtttgttcag ttatccgatt ctgatgactg
    11401 ccgacatcct tatgttcaac gccaacgaag tgcccgtcgg acgcgaccaa atccaacacg
    11461 tcgaaatggc gcgcgacatt gccggacgct tcaaccaccg tttccaagaa ctcttcaccc
    11521 tgcccgaagt gaaaatcgac gaaaacgtcg aactcttggt cggtttggac ggacgcaaaa
    11581 tgtccaaatc ctacggcaac accattccgc tttgggaaaa cgacaaaaaa acccaaaaat
    11641 cggtcaacaa aatcatcacc aatatgaaag agccgggcga gccgaaacag cccgacgaaa
    11701 gcccattgtt tgaaatctac aaagccttct ccacgccgtc tgaaacggca gaatttacga
    11761 aaatgcttgc cgatggcttg gcttggggcg aggcaaaaaa acttttggcg gcgaaaatca
    11821 acgccgaact cgccgaaccg cgcgaacgct acaacgagct gaccgccgac ccttcgcaaa
    11881 tcgaagagat tttgcaggca ggcgcgcaaa aagcgcgtaa agaagcacgc gaattgttgg
    11941 acaaagtacg cgatgcggtc ggcatccgcc cgttgaaata aacccgatgc cgtccgaacc
    12001 tcttaaaggc ttgccggaac aaaacgtcgt ccggctgacc ggcaagcatc ccggcgactt
    12061 ggaagccgtc agcggcaaat gtatggaaac cgacggaaag ggcgcacctt cgggctgggc
    12121 ggcaaacggc gtgtgccata ccttgtttgc caaactggtg ggcaatatcg ccgaagacgg
    12181 cggcaaactg acggattacc tgatttcgca ttccgccctg caaccctatc aggcaggcaa
    12241 aagcggctat gccgccgtgc agaacggacg ctatgtgctg gaaatcgaca gcgagggggc
    12301 gttttatttc cgccgccgcc attattgagg tattcggaca tcccggaata tatttgggtt
    12361 tttcaaaccc tgcaggaaaa agtccgcacc gtcgggaaac tcaaaaggag ggggatatgt
    12421 gttacaattt tccgaactgt tttcataaaa tagttttcgg acgtgtttca atatggcatt
    12481 gatgccgcct tattgttcga gaaaaaaacc tttatattta aatataattg ggttttaact
    12541 aaatgggaaa ccgttttctc tccggccgat gggcaaaatc agccgattga tggaacacgg
    12601 tccgattttt aagcaaaacc ttaatcaaca actcaagaag gataagacat gaaaaaatta
    12661 ttgattgccg caatgatggc ggctgccttg gcagcttgtt cgcaagaagc caaacaggag
    12721 gttaaggaag cggttcaagc cgttgagtcc gatgttaaag acactgcggc ttctgccgcc
    12781 gagtctgccg cttctgccgt cgaagaagcg aaagaccaag tcaaagatgc tgcggctgat
    12841 gcaaaggcaa gtgccgagga agctgtaact gaagccaaag aagctgtaac tgaagcagct
    12901 aaagatactt tgaacaaagc tgccgacgcg actcaggaag cggcagacaa aatgaaagat
    12961 gccgccaaat aatttgttgc cttggcaaaa atgatgggat gccgcctgcc ggcaacccaa
    13021 acgaaaccgc ctgaagattt tcaggcggtt tttgagttgt atgccccttg ttttttacgc
    13081 ctgcatgacc gttccgagca gatgtgccgc gcatatcggg atgttgcgga caggcaaatg
    13141 ccgtctggac agggtttgga cggaatttgt tccgtaaggt tcagacggcc tcgactttaa
    13201 acggcatatt gattttttgc cattggacgc tttcatattc caatgcgtcg gcaatcaggg
    13261 gatggcgttc cagccattcc ctgtcaatac gcaggatgaa gccgcagctt tccgtatccg
    13321 tgcgcaactg catatttttc gggaaagaca ggtcttggcg cgaacggcag aacagggcag
    13381 caaggcgcag ggacaaaacg gcataccaca acatttcgtt ggtgccgatg atgccgctca
    13441 tttttttcat atcgccgcga tgaccgatga ccagttgggc aagtatggtt tgttccttgc
    13501 gtgaaaagcc cggcatatcg gcgttttcga ggatgtaggc ggaatgcttg tgatagccgg
    13561 tgtgggcgat gtccaaaccg atttcgtgca gcgcggcggc gcgtccgaga tactgttgcc
    13621 acaaggcaag ctcttgaacc gtaacatttt tagcgtggca gaggctgtcc ataaaggttt
    13681 gcgcggtctc ggcggtgcgt ttcgcctgat tgaggctgac gtggtagcgg tgttggaact
    13741 cggcaaccgt ttgtccgcgc atatcttcgt ttaaaccgcg cccgatcaaa tcgtaaaaca
    13801 cgccgtcgcg cagggcggct tcggttacgg tcatcctgtc gagtttcatt tcttcaaacg
    13861 ccgccatcat cacggcaagt ccgccggcaa aaacttcgat gcgttccggt ttcaggtttt
    13921 caaatttggc ttttttgacc gaaccggctt cgatgatgcg ttcggcgagg gcgcgcattc
    13981 ctttgtaggt aatgtccgcc tcttggggca tttcggcggc aagcacgtcg cggatggatt
    14041 ttgccgaacc cgatgtgccg acggcggaat cccaaccttc gcgcctcata tttttgctga
    14101 tgcgctggat ttcgttgcgg gcggcggaaa tggcggcttg gaagtctttg gcgatgattt
    14161 tgttttggaa gaagcgcagg ctgtaggtta cgcagcccaa gggcaggctt tcagtaatgt
    14221 cgggattcag cgtcgagccg atgacaaatt ctgtcgaacc gccgccgatg tcgataacca
    14281 gcattttgcc gccgcccggg gggagggtgt ggatcacgcc ggtataaatc agtcgcgcct
    14341 cttcgcgccc ggcgatgatt tcgatgggga aacccagtgc cgcttcggct ttgggaagga
    14401 aatctgcgat gtttttggcg acgcggaatg tattggttgc cacggcgcgt acctgttcag
    14461 ggcggaagcc gcgcaggcgt tcgccgaatt ttgccagaca gtccaaagcc tgttcttggg
    14521 aagcggcact caggtttttc tgttcgtcca gtccggcggc gaagcgcacc atctgtttga
    14581 acgaatcgat gacttttaat tgtccgttgt tgttttcgca aatctggagg cggaaactgt
    14641 tggaacccaa atcgacggag gcgaggacgt ttgcgggatg ggtggtcatg gtggataccg
    14701 gtgggggaaa aacgcaatgt tactctgacg gcgcaggcgt tgacaataaa tgatgcggcg
    14761 gtttttgatt ctgcccacgg atgttgccga cggcattttt gcgcttattt gaaatccttt
    14821 tccacgctca tgaaaatctg cgtgtttttg cgtgtgtaaa aacttttcat attgctgtcg
    14881 attttcagat agcggaaatt gagttgcggc gtaaagccct tccaagagat tttgtcgtgc
    14941 cacaacgaca ggtttgcctg atattcgtgg tctttgcgcg ggaagcggta cacaatggtc
    15001 ccgggtgcgt caaacatcct gcgggtatag cgcaggtttg cccgcagacc caagcccgcc
    15061 gtcgaacgtt ttgactgcgc cgacacgcaa acccttgcgg acggaagcct gttccgcctc
    15121 tttcgtcatg ttgtgcgacc agtccgcacc gccgtaaagc agccagtctt taggcgcgga
    15181 atacatcagc gtcgcgcccg ccagcggcat atggctgtcg tatcgggcgg cggtgcggtc
    15241 ttcctgataa cgcttccaca tattgcccac gttcagcgtc agccgccagc gttcgctcaa
    15301 gcgtcgggag aaatcggcat tgaagccgcc gacgaaattg tatcggctgc cgcctaagag
    15361 gttttgcccg ataaacggca cgatgccgaa cgagcgcgtt accgaacgat ttttatagcc
    15421 gagcgacagg cgcaggcact gttcgctgaa atctttgtta tcccaataat gcacgccgcc
    15481 gccgctgatg ccgccgtaga ggaaatgatg cccgcccgca ttgatttcgc gcgacacgcc
    15541 taagccctag cgcaaaccgt gtgccttttg cggcaggctg tcggcggttt tcgtccagct
    15601 tctgcccgca aattcaatcg tttttttgga cgaagcgttg tttatag
//
LOCUS       DAWFJR010000042        14921 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWFJR010000042 DAWFJR010000000
VERSION     DAWFJR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14921
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.042"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(538..822)
                     /locus_tag="WGR04_001197"
     CDS             complement(538..822)
                     /locus_tag="WGR04_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0794753.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
     gene            complement(1250..2515)
                     /gene="ftsY"
                     /locus_tag="WGR04_001198"
     CDS             complement(1250..2515)
                     /gene="ftsY"
                     /locus_tag="WGR04_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ0794754.1"
                     /translation="MFSFFRRKKKQETPALEEAQIQETAAKAESETAQAIENIKEDAE
                     SLAESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWVARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
     gene            2660..4228
                     /gene="msrAB"
                     /locus_tag="WGR04_001199"
     CDS             2660..4228
                     /gene="msrAB"
                     /locus_tag="WGR04_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ0794755.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            complement(4330..4815)
                     /locus_tag="WGR04_001200"
     CDS             complement(4330..4815)
                     /locus_tag="WGR04_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ0794756.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGLLAAVFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLVGLIENGGDA
                     V"
     gene            complement(4841..5689)
                     /locus_tag="WGR04_001201"
     CDS             complement(4841..5689)
                     /locus_tag="WGR04_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0794757.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            complement(5783..6784)
                     /locus_tag="WGR04_001202"
     CDS             complement(5783..6784)
                     /locus_tag="WGR04_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0794758.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIAHFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVAINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            6843..7577
                     /locus_tag="WGR04_001203"
     CDS             6843..7577
                     /locus_tag="WGR04_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ0794759.1"
                     /translation="MQENPTVWLFDLDNTLHDADAGIFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMPETQNTLSR
                     LKGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTRTPPPRKYERMLCVKPSLS"
     gene            7553..7825
                     /locus_tag="WGR04_001204"
     CDS             7553..7825
                     /locus_tag="WGR04_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ0794760.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            7880..9250
                     /gene="glmU"
                     /locus_tag="WGR04_001205"
     CDS             7880..9250
                     /gene="glmU"
                     /locus_tag="WGR04_001205"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ0794761.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHEIGGETMLGRVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLSAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPNDPTGLGRIIRDSNGSVTAIVEEKDADAV
                     QKAVKEINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SHLAAGVNNKLQLTELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     CIFEGDIELGDNVEIGANCVIKNAKIGANSKIAPFSHLESCEVGENNRIGPYARLRPQ
                     ARLSDDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNIEDNKLALARARQTVIEGWMR
                     PEKDKQ"
     gene            9326..9655
                     /locus_tag="WGR04_001206"
     CDS             9326..9655
                     /locus_tag="WGR04_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ0794762.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVL
                     DANGAVLQNGDTVILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNISCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(9725..10990)
                     /gene="efeB"
                     /locus_tag="WGR04_001207"
     CDS             complement(9725..10990)
                     /gene="efeB"
                     /locus_tag="WGR04_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ0794763.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYNYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(11254..12420)
                     /locus_tag="WGR04_001208"
     CDS             complement(11254..12420)
                     /locus_tag="WGR04_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ0794764.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIETKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            complement(12417..13256)
                     /locus_tag="WGR04_001209"
     CDS             complement(12417..13256)
                     /locus_tag="WGR04_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ0794765.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMVLWMKKAARSMKQQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVMTWFLCGSRPSETLTRKE
                     ELK"
     gene            13478..14803
                     /locus_tag="WGR04_001210"
     CDS             13478..14803
                     /locus_tag="WGR04_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ0794766.1"
                     /translation="MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFF
                     RELTGSGNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
BASE COUNT         3458 a         4471 c         3745 g         3247 t
ORIGIN      
        1 catccgcacg gaaacctgca ccgcgtcatt cccacgaaag tgggaatcca gttttttgag
       61 tttcagtcat tcccgataaa ttgccttagc attgaatgtc tagattcccg cctgcgcggg
      121 aatgacgaat ccatccgcac agaaacctgc accgcgtcat tcccacgaac ctacatcccg
      181 tcattcccac gaaagtggga atctagaatc tctaaagctt cagctaacct ttgaatatta
      241 ctgttgttct aaggtctaga ttcccgcctg cgcgggaatg acggcatcgg tctgccgtta
      301 caacacggtt tctttagatt ttacgttcta gattcccgcc tgcgcgggaa tgacgaatcc
      361 atccgcacgg aaacctgcac cgcgtcattc ccgcgaacct acatcccgtc attcccacga
      421 acctaaaatc ccgtcattcc cacgaaagtg ggaatctagt tttttgagtt tcagtcattt
      481 ccgataaatt gccttagcat tgaatgtcta gattcccgcc tgcgcgggaa tgacgggcta
      541 aataatatca aaccataaat cctgccaaga aacattattt tcttcaatca gttgcaattt
      601 ccaagccctg ttccatttct tcaactgttt ttcccgagta attgcgctct ccatcgtagg
      661 atgcagttca taccaaacca gcatagtaac gttgtaccgt gatgtaaatc cctcaatcaa
      721 atgctcccta tgttggtaaa tacgttgcac caaatcagat gtaacgccaa tgtataacgt
      781 gccattacgt tggcttgcta aaatataaac cgcaggctgc atataatacc cttttgaatt
      841 atttaaattt atattcccgc gaacaccatc ccgtgattac tttaaccctt cgttattccc
      901 atagctttcc atcattcccg caactcttcg tcattcccac gaaagtggga atctagaacg
      961 caaaatctaa agaaaccgtt ttacccgata agtttctgca ccgacaaggc tggattcccg
     1021 cctgcgcggg aatgacggcg gagcggtttc tgttttttcg ataaatgccg caatctcaaa
     1081 tcccgtcatt cccacgaacc tacatcccgt cattcccacg aaagtgggaa tctaggacgc
     1141 ggaatctcaa gaaaccgttt acccgataag tttccgtgcc gatggttcta gattcccgcc
     1201 tgcgcgggaa tgacggcatc ggtctgctgt tttcggacgg catttcggct caatccagca
     1261 gtgcgtccac aaacgcgcgc gcgtcaaacg ggcgcaaatc gtctatgcct tcgcccacgc
     1321 cgatatagcg gacggggacg gggcggtcgg aagcaagcgc ggcgaggatg ccgccttttg
     1381 ccgtgccgtc gagtttggta acgataagcc ccgtcagccc taatgcgtcg tcaaaggctt
     1441 tgacttggtt gacggcgttt tgaccgatat tggcatcaag cacgacgatg atttcgtgcg
     1501 gcgcgtcggg catggctttt tgcagcacgc gtttcacttt tttgatttct tccatcaagt
     1561 gaagctgcgt gggcaggcgg ccggcggtgt cggcaagcac gatgtcgatg ccgcgcgctt
     1621 tggcggcttg gacggcatcg aagcacacgg cggcggaatc gcccgtggtt tgcgaaatca
     1681 cggttacgtt gttgcgttcg ccccaagctt gaagctgctc acgcgcggcg gcgcggaaag
     1741 tatcgccggc ggcaagcagc acagatttgc cctgcgcttg gaaatatttg gcgagtttgc
     1801 cgatggatgt ggttttgccc gcgccgttga tgccagcaag catgatgaca aacggctctt
     1861 tggtttcggg caaaaccagc ggtttctcta gaggcttaat caggtcgtac aaggcttctt
     1921 tcagcgcgcc gcgcaattcg ttgccgtctt tcagcccttt gaggctgacg cggtcgcgca
     1981 cgtctttcat caggtattcg gtggcttcca tgcccatgtc gctggtaatc agcacggttt
     2041 ccagctcttc gtacaaatct tcgtcgattt gtccgccgcc gaacacgccc gccagcgatt
     2101 tcgccatttt gtcgcgcgat ttggtcaaac cttgtttcaa acgcgccacc caaccgagct
     2161 tgtgttcttc agttgttgca acggcttctt gaacttgccc gacagcctcg ccgacggttt
     2221 cggcaatggc ttcttttgcc gcttcgactt gttccgctgc tttttccgcc gcttcctctg
     2281 cttcagacag catctcggca acggtttcct ttacctgttc aaccgcaccg ctgacggttt
     2341 caacagccga ttcgacctgc cctttgacgc tttctgctaa agattcagca tcttctttaa
     2401 tattttcaat tgcttgagcg gtttcggact ctgcttttgc tgcggtttcc tgaatttgag
     2461 cctcctcgag agccggcgtt tcctgttttt tcttgcgacg gaagaagctg aacattgaat
     2521 tttcctttta attttagaaa cttgaaacag ggcgtattgt agcgtatttt acgcggcaag
     2581 gttgtctgaa aatccgagct gtaaggtttc ggcatctcaa acgtctaatc atgcaaaccg
     2641 tccacacagg aaactcaaaa tgaaacaccg tactttcttt tccctttgcg ccaagttcgg
     2701 ctgcctgctt gcgctgggtg cttgttcgcc caaaatcgtc gatgccggag ccgcgaccgt
     2761 gccgcacact ttatccactt tgaaaactgc ggacaaccgc cccgccagtg tttacttgaa
     2821 aaaagacaaa ccgacgctga ttaaattttg ggcgagctgg tgtcctttgt gtctgtccga
     2881 attgggacag accgaaaaat gggcgcaaga tgcaaaattc agctccgcca acctgattac
     2941 cgtcgcgtct ccgggctttt tgcacgagaa aaaagacggc gacttccaaa aatggtatgc
     3001 cggtttgaat tatcccaagc tgcccgtcgt aaccgacaac ggcggcacga tcgcccaaag
     3061 cctgaatatc agcgtttacc cctcgtgggc gttaatcggt aaagacggcg acgtgcagcg
     3121 catcgtcaaa ggcagcatca acgaagcgca ggcgttggcg ttaatccgcg acccgaatgc
     3181 cgatttgggc agcttgaaac attcgttcta caaacccgac actcagaaaa aggattcaaa
     3241 aatcatgaac acacgcacca tttacctcgc cggcggctgc ttctggggct tggaagccta
     3301 tttccaacgc atcgacggcg tggttgacgc ggtatccggc tacgccaacg gcaacacgaa
     3361 aaatccgagc tatgaagacg tgtcctaccg ccatacgggc cacgccgaaa ccgttaaagt
     3421 gacctacgat gccgacaaac tcagcctaga cgacatcctg caatatttct tccgcgtcgt
     3481 tgatccgacc agcctcaaca aacagggcaa cgacaccggt acgcaatacc gcagcggcgt
     3541 gtactacacc gaccccgccg aaaaagccgt catcgccgcc gccctcaaac gcgagcagca
     3601 aaaataccaa ctgcccctcg ttgttgaaaa cgagccgctg aaaaacttct acgatgccga
     3661 ggaataccat caggactacc tgattaaaaa ccccaacggc tactgccaca tcgatatccg
     3721 caaagccgac gaaccgctgc cgggcaaaac caagaccgcc ccgcaaggca aaggcttcga
     3781 cgcggcaacg tataaaaaac cgagtgacgc cgaactcaaa cgcaccctga ccgaagagca
     3841 ataccaagtt acccaaaaca gcgcgaccga atatgccttc agccacgaat acgaccattt
     3901 gttcaaaccc ggcatttatg tggacgttgt cagcggcgaa cctttgttca gctccgccga
     3961 caaatatgat tccggctgcg gctggccgag cttcacgcgc ccgattgatg caaaatccgt
     4021 taccgaacac gatgatttca gctacaacat gcgccgcacc gaagtgcgca gccacgccgc
     4081 cgactcacac ttggggcacg tcttccccga cggcccgcgc gacaaaggcg gactgcgcta
     4141 ctgcatcaac ggcgcgagct tgaaattcat cccgctggaa caaatggacg cggcaggcta
     4201 cggcgcgttg aagagtaaag tgaaataagc cgcaccgccg cctaacccga caaaataccg
     4261 tctgaaacct gcaacgtttc agacggtatt ttttatccgg cggggatttt gtccagacgg
     4321 agattttgtt tagacggcat cgccgccgtt ttcaatcagc cccaccaacc gttccaacgc
     4381 gaaggcgacc gcctgcgcgc ggacggattc gcggttgccg tcaaaacggc gcattgcttc
     4441 gcaacttccg cccggaaagg caaacccgaa ccaaaccgtg ccgacgggtt tgctttcgct
     4501 gccgccgccc ggaccggcga tgccggaaat accgacggcg taatccgcct gcgccacggc
     4561 tttcgcgccg cgcgccatct catagacggt ttggcggctg accgcgccgt gttcgagcag
     4621 ggtttcgggc aacacgccca agcggtcttc tttggctttg ttgctgtatg ttacaaaact
     4681 ttgatcgaac cattgcgaac tgcctgcaac gcttgtgaat acggcggcaa gcagcccacc
     4741 cgtgcaggat tcggcacagc ttacggtttg acgttttttc gtcaggtttc gggcgatggt
     4801 gtgcagcgcg tccatttccc actcctcttt cagacggcgt ttaagaattg atgatgtgta
     4861 tgtcgcgttg cgggaacggg atgttgatat tgactttgcg gaggttttcg accacttgtt
     4921 cgttcaagtc gcattgcagc gtccagcggt ctgcttcgtt tgcccaagcc cataatgtga
     4981 tttcgatggc attgtcgccc aaggcggtga tgtaggcggc ggcctgccgc tcttcgtttt
     5041 gaacgctcaa ggggtgttcg acggcggctt tcaacaccgc ctctttcgcc actttcaaat
     5101 cgcagttgta atcgacgccg actatcactt gggcgcggca cagcggcagt gtggaacggt
     5161 tgacgatgct gttgcccatc accacgctgt tgggcagcac gacttcttcg ttgtcggtcg
     5221 tccgcaaaga agtctgcacc attttaatct ctcggacata tccttcaaaa ccgccgacgc
     5281 ggataaaatc gccgactttg aacgggcgga acaggataat cagcgcgccg gcggcaaaat
     5341 tggacagctg gtctttcaag gacaacgcca ccgccaaacc cgcgccgccg attaaggcgg
     5401 ttacggatgt tgtggaaacg cccaatctgc ccaatgcggc aataatcacc aaaatcaata
     5461 agccgatatt ggcaacatta cacaaaaaac taatcagcgt ggcatcgacc tgcgcgcgcg
     5521 tcatcgccgc cctcatcaca gcgacaatgc gtttcgccgc ccattttccg accaaaaaaa
     5581 taagcagcgc ggcggcaagg ttcagcccga acgcccacgc cttttcagcc agatgctccc
     5641 aaccggaaac actgatcagg tgtaaaaaat caaattgttt gaagtccatt gttttcctct
     5701 tgatcgaaca tccgccccat gcggcgtaat cggcacaggt gtaaaaatgc cgtatgaagc
     5761 cctgcggggc ggtatgtttg ttttatttca aaaccgttct aatccaacgg gaaacccatc
     5821 cgcgctggtt cgcatcaata tcgcgctgcg cgggggcggt ggtgtgcgtc ggggcttggg
     5881 cgaagcggaa cacgtcgggc aggcgcgtgt ttttgacggc ggggtaaacc cacatttcgg
     5941 acggaaccgc ctgctgcact tcccgacttt gcagccattg caccagtttt gccgccagct
     6001 tcggctgttt cgcgcccttc aagactgccg cgccttcgac ctggcggaat acgccgcctt
     6061 ttaaaaacag gttgcccgtc ggcggctcgc tgtatttgcc tttggaaaaa tacacttccg
     6121 ccgccggact ggcggcataa ccgaccacca gcggatacgc gccgccgttg tgcgaaaagt
     6181 cggtgtaata cgcctcgctc cagcctttgg cgaccttcac gccgttctgc cgcatctgtg
     6241 cccaccattt gaacgcgcct tcttcgccca gaccgctgat gttcgccatc aggaagccca
     6301 gccccgggga cgacgtggcg ggagacggta cgaccaatag gtttttatat tcggggcggg
     6361 tcaaatcctg cagggtttgc ggcaggggca gctttttgcc ttcaaaccat tttttgtcgt
     6421 aattgatagc cacatagccg taatcgaccg ccaaagccga aggcagcccg accgcgacgg
     6481 gggcggattc gggttgcgcc gccgccaaaa tgcccatttc ccgcgccttg ccgatattgg
     6541 cgttgtccaa accatacaca gcgtcggcaa tcgggttggc gcggctcaaa atcagtttgt
     6601 tgagcatttc gttcgcgccg cccgcctgaa taatcgacac cttcgcatcg tttgcccgct
     6661 cgaaatgcgc aatcagccct ttgggcaggc tgaacgactt atgcaccgcc agcctgactt
     6721 ccgtctgcgc ctgcaggtat gccgaaaccg ccagcagcgg cagcagccaa attttccgtt
     6781 tcattccgag tcctcctcta ttcgctgtaa aataacattc taacaaattt tcacggttca
     6841 aaatgcaaga aaacccgacc gtgtggctgt tcgacctcga caacacgctg cacgatgccg
     6901 acgcaggcat cttcacactc atcaaccgcg ctatgacacg ctatatggca cgccgcctca
     6961 aactctccga atctgccgcg tccgacctgc gtcaagacta ttggcaccgc tacggcgcaa
     7021 cgctcgccgg actgcaaatc caccatcccg aaatcgacat cgccgaattt ttgcgcgaaa
     7081 gccatccgat cgatgcaatc ctgaccaggc tgcacggcat gcctgaaaca caaaacaccc
     7141 tgagccgcct aaaagggcgc aaggcggttt tttccaacgg cccgtcgttt tacgtccgtg
     7201 ccgttgtcgg cgcactcggt ttggaaaacc gtttcgacgc actgttcggc acggatgatt
     7261 tcgggctgct gtacaaaccc aatccgcaag cgtatctcaa tgtctgccgc cttttggacg
     7321 taccgcccga atgctgcatt atggtggacg acagcgcgga caacctgcat caggcaaaag
     7381 cattgggcat gaaaaccgtc cggttcggtg caaaagtgca cgacctgccc tttatcgatg
     7441 cttccgtaag cgatatggcg caactggctc ggtatgcaga aactttgtca gaacaccgcc
     7501 aaaatcatta caatacccgc accccccccc cccgaaaata cgaaagaatg ttatgcgtaa
     7561 aaccttcctt atcctgatga ctgccgccgc ccttttgtcg ggctgcgcgt gggaaactta
     7621 tcaagacggc aacggcaaga ccgccgtccg tcaaaaatat cccgccggca cgcccgttta
     7681 ttaccaagac ggcagctact cgaaaaatat gaactacaac caataccgtc ccgaacgcca
     7741 tgccgtgtta cccaaccaaa ccggcaacaa cgccgacgaa gagcatcgcc aacactggca
     7801 aaagcccaaa tttcaaaacc gataaaccca ccctatgccg tctgaagccc tttcagacgg
     7861 cattgcacag gaaaacgcta tgccgcaaaa cactttaaac atcgtcatcc tcgccgccgg
     7921 caaaggcacg cgcatgtatt ccaaaatgcc caaggttttg cacgaaatcg gcggcgaaac
     7981 aatgctcggg cgcgttatcg acaccgccgc cgcgctgaat ccgcaaaaca tctgcgtcgt
     8041 cgtcggccac ggcaaagagc aagttttgga caccgtcaaa cgcgatgtcg tctgggttga
     8101 acaaaccgaa cagctcggta ccggccacgc cgtcaaaacc gccctgcccc acctttccgc
     8161 cgaaggccgc acgctggtgt tgtacggcga cgttccttta attgacgttg aaaccctcga
     8221 aaccctgctc gaagccgcag gcaacgaagt cgggctgttg accgacgttc ccaacgaccc
     8281 gacaggcttg gggcgtatca tccgcgacag caacggcagc gtaaccgcca tcgtcgaaga
     8341 aaaagacgcc gacgccgtcc aaaaagccgt gaaagaaatc aataccggca tcctcgtcct
     8401 gcccaacgcc aaactcgaaa actggctgaa cagcctttcc agcaacaatg cacaaggcga
     8461 atactacctg accgacctca tcgccaaagc cgttgccgac ggtattaaag ttcatcccgt
     8521 ccaagtgcgc gcctcccacc tcgccgccgg cgtgaacaac aaactccagc tcaccgaact
     8581 cgaacgcatc ttccaaaccg aacaggcgca agaattgctc aaagcaggcg taaccctgcg
     8641 cgatccggca cgtttcgatt tacgaggccg tctgaaacac gggcaagacg tcgtgattga
     8701 tgtgaactgt atctttgaag gcgacatcga gctcggcgac aacgtcgaaa tcggcgcaaa
     8761 ctgcgtcatc aaaaacgcca aaatcggcgc aaacagcaaa atcgccccct tctcccacct
     8821 cgaaagctgc gaagtcggcg aaaacaaccg aatcggcccg tacgcccgcc tgcgtccgca
     8881 agcccgcctt tcagacgacg tacacgtcgg caacttcgtc gaaatcaaaa acgccgccat
     8941 cggcaaaggc accaaagcca accacctcac ctacatcggc gatgccgaag tcggcagcaa
     9001 aaccaacttc ggcgcgggta cgattattgc caactacgac ggtgtgcaca aacacaaaac
     9061 cgttatcggc gacgaagtcc gcatcggttc aaactgcgtc ctagtcgccc ccgttaccct
     9121 cggcaacaaa gtaaccacag gcgcaggcag cacgattacc cgcaatatcg aagacaacaa
     9181 actcgccctc gcccgcgccc gccaaaccgt catcgaaggc tggatgcggc ctgaaaaaga
     9241 caagcaataa agctatgccg tctgaagccg gtttcaggct tcagacggca tcccaaaaac
     9301 aaacatccga taaggacgga aaactatgtc attaccccct tgcccgcaat gcgcctccga
     9361 atacacctat gaagacggcg gacaatacat ctgccccgaa tgcgcccacg aatggaatga
     9421 aaccgaatcc gccgccgacc ttgcggctca agtgctcgat gccaacggcg cagtgctgca
     9481 aaacggcgac accgtcatcc tcatcaaaga cctcaaggta aaaggcagct cgatgacgat
     9541 caaacaaggt acaaaagtca aaggcatacg cctgcaggaa ggcgatcaca acatctcctg
     9601 caaaatcgac ggcagcgcga tgaatttaaa atctgaattc gtcaaaaaag cctgaccgcc
     9661 caaacactaa aatgccgtct gaacccctta tcggattcag acggcatttg tccgtcaggc
     9721 aggtttatac gcccagcagc ccttgtccca agaagccgcc tttttccacg ccgggcagca
     9781 cgaagaaata gccgccgccg aaggggctga tgtattcttc cagcggttcg ccgttgagga
     9841 ggttttgcac gaagatgaat ccgtcggcaa ggtttgcctg ataacagacg aacaccagcc
     9901 cgacatcaag ctgtccgctt gaggcgagtc cgcgcgaata gttgtaggcg cggcggaaga
     9961 ggcggtgttt tttaaggaac tcgggatcgc gcggattcgc caggcgtata tggctgtctt
    10021 tgggcgtggt attcccctcg gggtctttgg caaaatccgg ttggtcggct tcttttttgc
    10081 cgtccatcgg cgcgccgctg tatttgcgcc gcccgaaaat gtcggtttgc tcttgaagcg
    10141 gcgtcctgtc ccaaaactca acaaagtggc ggataaggcg gactgcctga tagctgccgt
    10201 ttttcgccca ctccggttcg tcgaggctgt tggcggccac ccccgtccac aaaacctcgt
    10261 cggcagtttt ggggtcggaa actttggggt tgcccgtgcc gtcgcggaag cccaacaggt
    10321 tgcgcgccgc catcgcgccg ggttcggatt taggctgcca cccgtcgata ctccagcgga
    10381 taacggcggt ttggacggtg tgtttgatga tgtcgcgcag ggcggcttgg caggtttcgg
    10441 gggtgaaggc acagatttgc aggctcaaat cgccgtcgca ccagcttttt tgcagcttat
    10501 cgttggagaa gtcgcgcatt tcctgcaaat gaatcggttt tttgtctttg agtccgaacc
    10561 ggccgtcaaa caggctgctg cccaccccca cggtaacggt caacccgtcg gggttgaagg
    10621 ctttgcccaa aatgccgctg ccggctggcg gaagtttgtc gtcgccgtct tggtattcgc
    10681 cgccttgggt gagaaactcg atgcgggcgg tcagcgtgcg gaacaggttt tccagctgct
    10741 tggcactttg cgcggttacg tcgaaggcgc acataatcga aaacgcctgc tgcggcgtaa
    10801 cgatgcctgc ctgatgttcg ccgtagcagg gataggcttg gggcgagtgt tggctttcgg
    10861 cggtgcgttc ggcggtttcg ccctgttttt tgccgccgag ataacctccg attgcgccga
    10921 ctgcgccggc tgcgatcgcg gttttaaaaa gagtgcgcct ggtcggttgt gcgggttggt
    10981 ttttgctcat ggtgtttcct tcaatatccg tgcagcagga tgggtatccc tgcacgccgt
    11041 ttttccatta accgatagcg gcaggttgaa atcccgccct gcaatatatg gcggatatag
    11101 tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtataaat agtacggcaa
    11161 ggcaaggcga ggcaatgccg tactggtttt tgttaatcca ctataaaact tcagacggta
    11221 tctgccgcca aatgccgtct gaacgcttgc ggcttatttc aagccgagta tgccgcgaag
    11281 ttgggcaagg tcttcggcaa gcgcgttaat agaggcctgt aacgctttgc ggtcggcttc
    11341 gcccagcttg tcgtaggttt caaaaccgtc tttagtccgg tatttcgcca gaatttcgtt
    11401 gacctgtttg aagttggtat cggttttttc caacaaggct ttgtttttgg tctcgatcaa
    11461 cggacggaac aaatcgacga tttttttaga tccgtccaca ttggcttgga agtcgctcaa
    11521 atcggtgtgg ctgtaccggt cttcttcgcc gctgatttta ctgcccgcca cttcttcaat
    11581 cagttcggac gcgccgccga ccaccttgcc cggaggaaac gccaatgcgt cgatttcttt
    11641 ttgcagggct tcgacatcgg tcatcagttt cgctgcaatt tccttcacgc cggacacgtc
    11701 tttttccacc caaagggcgt attcgatacg gtggaagccg gtaaatccgg catctttcgc
    11761 gccgtctttg aagtcgtctt cacgcgcatc gatgacgggg tcgagttcgc tgaaaagctc
    11821 ggcaatcggt tcgatgcgtt cgtaatggac gcgggtgtcg gcaaacaggg atttcgcctt
    11881 ttcaatgtcg cctgctttga cggcttcggt aaaggttttg gttttcgcca ccagctcttt
    11941 aacctcgcct tgaacgtagg ctttatagtc ggcgagcggt tgggacagtt tttccaaatc
    12001 cgcttcgttg gcggtgtctt taaagccgct gtcggttacc accagcttgc cgcgcggatt
    12061 ggtcaaaaga ccgcaagtca tttcgtattc gcccggcaac agggtgacgg tcattttatc
    12121 ggaaagtccg ggggcgatgt tttcgcgctc gtccaccacc atcacgcctt tcaggatttc
    12181 ccattcgagc ttgcggccgc tgttgttttt aatattgaac acaacctgtc cgctcggcac
    12241 ggtcagttcc atcggttcgc aggcattgtc gttgacggcg atactgaccg aaccgccctc
    12301 gttggcggtt tgcgcctcac cggacgcggc cggcgcagct ttctccgcct ccggcggctg
    12361 gcacgcggtc aaacctaagg caagcatcac ggacaatgcg gtcaaattga attttctcat
    12421 ttcagctcct ctttacgggt taaagtttca gacggcctgc tgccgcacaa aaaccaagtc
    12481 atgacgggaa taaggtacag cagccaaacc aaggcctcgc cctgcgtcgg atggtcggta
    12541 tagccgaaaa atccgccgag cagcacacct aacggactgt cttcgtgcaa atattttgat
    12601 gagtcgaaca caatgtcctg aagcgcgttc caaatgcctg cttcgtgcag cgcgcgcagc
    12661 gagccggcaa gcaggccggc ggcaacgaca atcagaaacg cccccgtcca acggaaaaac
    12721 ttcgccagat tcaggcgcat cccaccctga taaatcaacg cgccaatcac ggcggcagcc
    12781 aaaacccccg ctaccgcacc ggccggcatc tgccaagtcg ggctctgttt gaatacggca
    12841 agcagaaaaa aaacgctctc caaaccttcg cgcgccacgg caagaaacgc cataccgacc
    12901 aaggcccatc cttgtccgct gccccggttc aaagccgcct gcacagaatc ctgaagctgc
    12961 tgcttcatcg aacgcgccgc ctttttcatc cataaaacca tataagtcag catcgcgacg
    13021 gcaaccaggc cgataatgcc gacgacgaac tcctgctgct tttggggaat ctcgcccgtt
    13081 gccgaatgga tgccgtaccc cagccccaaa cacatcaaag aagcaaggac gaccccgaac
    13141 cagaccttag gcatcagttt ggaatgtccg gactgtttca gaaaaccggc aacgatgccg
    13201 acaatgagcg cggcttcgat accctcgcgc aacataatta aaaaagcgac cagcataaac
    13261 gcgaacgaac aaggatgatg aataatatat tatcggaata ttttcattgc ttgtaaatac
    13321 aaatgcaagt tatttttatc tgcagtaccg cgcggcggaa agttccgcag gctgcagctg
    13381 cgccctgtgt taaaatcccc tctccacggc tgccgcaacg ccgcccgaaa ccatctttct
    13441 tattactgcc ttattactgc cggcaacatt gtccattatg aaaaaatacc tattccgcgc
    13501 cgccctgtac ggcatcgccg ccgccatcct cgccgcctgc caaagcaaga gcatccaaac
    13561 ctttccgcaa cccgacacat ccgtcatcaa cggcccggac cggccggtcg gcatccccga
    13621 ccccgccgga acgacggtcg gcggcggcgg ggccgtttat accgttgtac cgcacctgtc
    13681 cctgccccac tgggcggcgc aggatttcgc caaaagcctg caatccttcc gcctcggctg
    13741 cgccaatttg aaaaaccgcc aaggctggca ggatgtgtgc gcccaagcct ttcaaacccc
    13801 cgtccattcc tttcaggcaa aacagttttt tgaacgctat ttcacgccgt ggcaggttgc
    13861 aggcaacgga agccttgccg gtacggttac cggctattac gaaccggtgc tgaagggcga
    13921 cgacaggcgg acggcacaag cccgcttccc gatttacggt attcccgacg attttatctc
    13981 cgtccccctg cctgccggtt tgcggagcgg aaaagccctt gtccgcatca ggcagacggg
    14041 gaaaaacagc ggcacaatcg acaatgccgg cggcacgcac accgccgacc tctcccgatt
    14101 ccccatcacc gcgcgcacaa cggcaatcaa aggcaggttt gaaggaagcc gcttcctccc
    14161 ctaccacacg cgcaaccaaa tcaacggcgg cgcgcttgac ggcaaagcac cgatactcgg
    14221 ttacgccgaa gaccccgtcg aactcttttt tatgcacatc caaggctccg gccgtctgaa
    14281 aaccccgtcc ggcaaataca tccgcatcgg ttatgccgac aaaaacgagc atccgtatgt
    14341 ttccatcgga cgctatatgg cggataaggg ctacctcaaa ctcggacaaa cctcgatgca
    14401 gggcatcaaa gcctatatgc ggcaaaatcc gcaacgcctc gccgaagttt tgggtcaaaa
    14461 ccccagctat atctttttcc gcgagcttac cggaagcggc aatgacggcc ctgtcggcgc
    14521 actgggcacg ccgctgatgg gggaatatgc cggcgcagtc gaccggcact acattacctt
    14581 gggtgcgccc ttatttgtcg ccaccgccca tccggttacc cgcaaagccc tcaaccgcct
    14641 gattatggcg caggataccg gcagcgcgat taaaggcgcg gtgcgcgtgg attatttttg
    14701 gggatacggc gacgaagccg gcgaacttgc cggcaaacag aaaaccacgg gttacgtctg
    14761 gcagctccta cccaacggta tgaagcccga ataccgcccg taaaccggaa aacaatgcca
    14821 tctgaagccc atccctaggc aatcgggcgc attaaggcga agttttgcga taccgcaggc
    14881 acaagccggc cggtcggaca aaacgccttt cttatttgac g
//
LOCUS       DAWFJR010000043        14829 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWFJR010000043 DAWFJR010000000
VERSION     DAWFJR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14829
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.043"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..173)
                     /locus_tag="WGR04_001211"
     CDS             complement(<1..173)
                     /locus_tag="WGR04_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0794767.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDRVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPLL"
     gene            360..539
                     /locus_tag="WGR04_001212"
     CDS             360..539
                     /locus_tag="WGR04_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794768.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYERRLGLG
                     AKLKNKNPDTDEKRQ"
     gene            complement(753..1754)
                     /locus_tag="WGR04_001213"
     CDS             complement(753..1754)
                     /locus_tag="WGR04_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ0794769.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSQSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEAYEPPAHWEWIRKIRDTVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPLARLKQWLGMMKKEFAAAQNLFDRVRTVKDADEVRNILAEFEREMNT"
     gene            complement(1831..5664)
                     /locus_tag="WGR04_001214"
     CDS             complement(1831..5664)
                     /locus_tag="WGR04_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ0794770.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETIQKRHQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAVPLDANSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLACWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGLNIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKHTRTVCMEFFGTVATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKARWEWLLANLDAPLADYKARYGSA
                     VHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSIKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFEPAA
                     AMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIRNPASPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASNLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5925..6021
                     /gene="ffs"
                     /locus_tag="WGR04_001215"
     ncRNA           5925..6021
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WGR04_001215"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            6188..7879
                     /gene="dld"
                     /locus_tag="WGR04_001216"
     CDS             6188..7879
                     /gene="dld"
                     /locus_tag="WGR04_001216"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ0794771.1"
                     /translation="MSASQLLSRLTQTVGEKYIITEPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINDNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            8011..8118
                     /locus_tag="WGR04_001217"
                     /pseudo
     CDS             8011..8118
                     /locus_tag="WGR04_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            8174..8545
                     /locus_tag="WGR04_001218"
     CDS             8174..8545
                     /locus_tag="WGR04_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ0794772.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            8700..9248
                     /locus_tag="WGR04_001219"
     CDS             8700..9248
                     /locus_tag="WGR04_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0794773.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            9382..10473
                     /locus_tag="WGR04_001220"
     CDS             9382..10473
                     /locus_tag="WGR04_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0794774.1"
                     /translation="MNAQTLIANVAEFVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNRYLDDGYDSLKTRLD
                     GAWAETERLAGLAWSGTPDNLTTLKLREAAAVLTLAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            10928..11098
                     /locus_tag="WGR04_001221"
     CDS             10928..11098
                     /locus_tag="WGR04_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ0794775.1"
                     /translation="MAQYMCGPCGWIYDEELGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
     gene            12399..12579
                     /locus_tag="WGR04_001222"
                     /pseudo
     CDS             12399..12579
                     /locus_tag="WGR04_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697910.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12723..14501
                     /locus_tag="WGR04_001223"
     CDS             12723..14501
                     /locus_tag="WGR04_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ0794776.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVATAVLATLLS
                     ATVQASTTDEDDEELEPVVRTAPVLSFHSDKEGTGEKEVTEDSNWGIYFDNKGVLKAG
                     AITLKAGDNLKIKQNTNENTNDSSFTYSLKKDLTDLTSVETEKLSFGANGNKVNITSD
                     TKGLNFAKETAGTNGDTTVHLNGIGSTLTDTLLNTGATTNVTNDNVTDDEKKRAASVK
                     DVLNAGWNIKGVKPGTASDNVDFVRTYDTVEFLSADTKTTTVNVESKDNGKRTEVKIG
                     AKTSVIKEKDGKLVTGKGKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQT
                     GQADKFETVTSGTKVTFASGNGTTATVSKDDQGNITVKYDVNVGDALNVNQLQNSGWN
                     LDSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMTPQFSS
                     VSLGAGADAPTLSVDGDALNVGSKKDNKPVRITNVAPGVKEGDVTNVAQLKGVAQNLN
                     NRIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDGG
                     NWIIKGTASGNSRGHFGASASVGYQW"
     gene            14634..>14829
                     /locus_tag="WGR04_001224"
     CDS             14634..>14829
                     /locus_tag="WGR04_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0794777.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVL"
BASE COUNT         3739 a         3972 c         3881 g         3237 t
ORIGIN      
        1 agcagcggat aggcgggacg gccgcggtgg tctcggaggt aacgggtttt ttgacgattc
       61 aggtattgtt cgatcggctg ccaatcaatc acccggtcca atttcaatag cgggaaacgg
      121 tcgatgtgtt tggcaatcat ggcttgggcg gtttgttgga agaaggtgct catgagaaat
      181 cccctaaatg tcttggtggg aatttagggg attttgggga attttgcaaa ggtctctatt
      241 tgtactgtct tcggctcgcc gccttgtcct tatttaaatt taattcacta tatttgcccg
      301 tgcgtttgtg ataaattcgg cactgtattt gatatgattt taacaaatcg ggaggggcaa
      361 tgttttggta tgtgattggc ttttgcgcct ttgtcgtcgc gctgctgtcg ctgtgggtca
      421 atgccggcgc gttcggtatg caggaagacg ataccccgca atcggattat gaaaggcgtt
      481 tgggcttggg tgcgaagctg aaaaacaaaa atccggatac ggacgaaaaa cggcaataaa
      541 gacaacaaat gccgtctgaa aggttttcag acggcatttg ctttggagcg gatatagtgg
      601 attaacaaaa atcaggacac ggcgacgaag ccgcagacag tacaaatagt acgaaaccga
      661 ttcacttggt gcttcagcac cttagagaat cgttctcttt gagctaaggc gaggcaacgc
      721 tgtactggtt tttgttaatc cactatacat attcaagtat tcatttctcg ctcaaattca
      781 gccaagatgt tccgaacttc gtccgcatcc ttaaccgttc ggacgcggtc gaacagattt
      841 tgtgctgctg caaattcttt cttcatcata cccagccact gtttcagccg cgccagcgga
      901 tatttgttgt ttgcctcttt tgtcaggcac agctcgaaaa actgccgtat ccatttggaa
      961 acttcggcaa aatccgtgtc tttgaccggc ccgccgttct cgtattgctt gatttgccgc
     1021 gccaaatcgg ggcggatgac cgcgccgcga ccgagcatca cgctgttgca gccgctgatt
     1081 gttttgatgc cgatatagtc ctgcaggctg aaaacgtcgc cgttggcggt aacgggaata
     1141 ttgacggtat ctcggatttt ccttatccat tcccaatgcg ccggcggttc gtaagcctcg
     1201 gctttggtac gcgcgtgtac ggtcagtccg catgcgcccc cttcggcaat cgcacaggcg
     1261 cattccaaag cccggctttt atcttcatag ccgagccgca tttttgcggt gagcggaata
     1321 tgtgcgggca aacgtccgcg cagcgttttg acgatgtgga atatcagttc cggctctttt
     1381 aaaaggattg cgccgccttt gtgtttgttg acggtgggcg cggggcagcc gaagttcaaa
     1441 tcgattttgt tcgcaccgaa gcggacggct tccagcgcat tcgccgccat attgtccgca
     1501 tcgctgccca aaagttggac ggtgcagggc gtgccggaaa acgttttgtt tccgttggcg
     1561 atttcgggga catattttaa ccatatggat tgggaatgca cggtatgggt aatgcgtaca
     1621 aattcgctga cgcattcgtc gtagccgcca atacgcgtca gcaggtcgcg catcacgtca
     1681 tcgaccagac cctgcatagg ggcaaggatg attcgggttt tttgtttggg ttcggttgtt
     1741 tgtccgtcaa tcatgatttg ggtaggtatt gggtttaaac aatgccgtct gaaggctttc
     1801 ggcgttcaga cggcatttcc gtgtccgttg ttacagcaat actttttcca cgccgccgtt
     1861 gttggctttt ttcacaaact cgtccagcca gttttcgccg aggatgtgtt tcgccatttc
     1921 gataacgatg tagtcggcag gcatattgtt gtcgtcggcg tagcggctca aaccttgcag
     1981 gcaggcgggg caggaggtca gcattttgac gggttcgccc tgcggcagct ctttgaggtt
     2041 tttctcgatt tcttcctgtt tgcggaattt gacttgggtg gcgatgtcgg ggcgtttgac
     2101 ggcaaacata ccggattcgc cgcagcagcg gtcgcttaaa acgactttct gccccatcag
     2161 gttgcttgcc atttgggtgg cgttcatggt tttgatgggt gtgtggcagg ggtcgtggta
     2221 gaggtattgc tgacctttca cgccgtcgag tttcacgcct ttttcaagca ggtattcgtg
     2281 gatgtcgatg atgcggcagc cggggaagat ttcctcaaat cggtattttt cgagctggtc
     2341 gtaacaagtg ccgcaactga cgacgacggt tttgatgtcg aggtagttga gggtgtttgc
     2401 catacggtgg aaagccacgc ggttgttggt gctcatttct tcggcttttg ccttattgcc
     2461 gcccgcgtcc tgcggatagc cgcaacacat atagccgggc ggcaggacgg tttgcacgcc
     2521 gacgtgccag agcatggctt ggacggcgag tccgatttgg ctgaacaggc gttccgaacc
     2581 acagccggga aagtaaaaca cggcttcggc atcttccgga gaagccggat tgcggatgat
     2641 ggggatgctt ttgccgtctt cgatgcccaa taaggagcgc ggtgttttag cgggtacgct
     2701 tttgggcagt gggcggttga tgaaatggat aacctgttct ttaatcgggg ctttgccgac
     2761 ggttgccttg ggttcggctt tttgcttttt cgtgccgatc ggaagaagct tgccgatttt
     2821 gtaggcaaag ttttgcgcgg ggaagccggt ttgtatcatg gcggcgcgaa gggctttgat
     2881 ggttttcggg ccggtggcgt tcaaaaacgc catacccata gctgcggcag gctcaaatcg
     2941 tttgtggccg gaatcggcaa ggtagttgcg gacggctacg gttacgtcgc cgaagtcgat
     3001 gttgacgggg cagggtttga cgcagcggtg gcacacggtg cagtggtcgc cgatgtccat
     3061 gagttcttca aagtgtttga tggaaacgcc gcgacgggtt tgttcttcgt ataagaaggc
     3121 ctcggtcagc aaacccacgc cgaggatttt gttgcggggg ctgtacagca ggttggcacg
     3181 cggaacgtgg gtggagcaga cgggtttgca tttgccgcag cgcaggcagt ctttgacgga
     3241 atcggcaatc gtgccgaggt ctgatttttc cataatcagc gattccgcgc ccaaaagctc
     3301 gaaagacggc gtgtaggcgt tgcgtaagtc cgagcctttc atcagtttgt gacggttgaa
     3361 ggtgtgtttg ggatcgactt ggtttttgta gtcccaaaac ggctgcattt cttcgtcgct
     3421 taaaaattcg agcttggtga tgccgatgcc gtgttcgccc gaaatcacgc ctccgagcga
     3481 acgggcgatt ttcataatgc gttcgactga gcggtatgcc gtctgaagca tttcggcatc
     3541 gtctgagtta accggaatat tggtgtgaac gttaccgtcg ccggcgtgca tatgcagggc
     3601 gacaaagacg cggctgcgta cggtttttgc gtggattttt cccaagcctt ggataatttt
     3661 ggtgtcggtt ttgccgctga agatttcaga aagcggcttc attacgtccg ctttgacaga
     3721 cacgcgcagg cggaaatcgc ggaatgcggt aaagcagctt tcattgtctt tggcttcggg
     3781 cgcggcgtgg acggctgagc catagcgtgc tttatagtcg gcaagcggcg cgtccaagtt
     3841 ggcaagcagc cattcccaac gcgctttgac ggcggaaacg tgggcaaggg cgtgtttgcc
     3901 gcgttcgccc aacagttcgg cggtcggcag gtctgttccc attttgtcga tggggagttt
     3961 gcccgaaaga tattgctcca atgcggcaca gagtttgagt ttgttttgga tggaaagctc
     4021 gatgttgatg cgttcgatgc cgtctgaata ctcgccgagc ctttcgagtg ggatgaccac
     4081 gtcttcgtta attttaaagg cgttggtgtg cttggcgatg gcggcggtgc ggctgcggtc
     4141 gagccaaaag gttttgcgcg cttcgggaga cacggcgata aagccttcgc catcgcgggc
     4201 gcgggcaagt tcgcaaatgt gttcggcggc tgcctctacg gcggcttcgt cgtcggaaac
     4261 cacgtccgcc agcaagacca ttttcggccg tcccttgccc gccgctttgg ttgcgtagcc
     4321 gacggcgcgg acgtaacgcc agtccaaatg ttccaaaccc gccagccgca cgctgtcgtg
     4381 ggcaagcagg aaatcgcgga tttcgacgat agaaggcgta gcggtggcga ccgtaccgaa
     4441 aaactccata cacacggtgc gcgtgtgttt cggcattttg tgcaacacga aggcgacgct
     4501 ggtgatgatg ccgtccgtgc cttctttctg cacgccgggt aggccgctca agaatttgtc
     4561 ggtaacgtct ttgcccaaac cgactttgcg gaatttgtgg ccgggaattt ctaagcgttc
     4621 ggttttaacg atattgaggc cgtctgaatc cagcgtgtga acgtcgaata cggcggtttc
     4681 ttcgtcgtgg attttgccga aattgtggcg cacgcgctcg atgcgcagcc attcaccctg
     4741 cgggttgacc atctgccagc aggcaaggtt gtctaacgcc gtcccccaca gtacggcttt
     4801 tttgccgccc gcgttcatcg ccacattgcc gcccacgcag gacgcgtcgg cggaagtcgg
     4861 atcgacggcg aacaccaaac ctgcctgatg cgcggtttct tccactcgtc gggttactac
     4921 gcccgctccg caccggataa tcggatgctt gccgtccaag cccgccagct caacgtattc
     4981 aacaccacga tgcttgtcga gtttttcggt attgatgacc gcgctgttcg cgtccaaagg
     5041 taccgcgccg ccggtataac ccgtgccgcc gccgcgcggg ataataacca agtccaattc
     5101 gattaaggcg cgcaccaaag gcgcgatttc cgcctccgtg tcgggattga cgacgacaaa
     5161 aggatattcg acgcgccagt cggtcgcatc ggtaacgtgc gttacgcgcg ccaatccgtc
     5221 aaacatgatg ttgtgcggct tggtaatttt gcttaagcgt tccaaaattt gatggcgttt
     5281 ttggatggtt tcatcaaaac tgctatcaaa acgctccact gctttttctg ctgcggcaac
     5341 caacacatcg acttgccgat tatcgtcgcg gcgtttgcgg atttcgttca acctgtggcg
     5401 catttcgcgt accagcgcgg cgcggcgttt cggatgctcc agcaaatcat cgaccagata
     5461 cggattgcgt acgaccaccc aaatatcgcc caatacttca aacagcatcc gtgccgaacg
     5521 cccggtttta cgctgtccgc gcaggtcttg caggatttgc cacgcttcgt cgcccaataa
     5581 tcggatgacg atttcgcggt cggtataaga agtatagttg tagggaattt cccgaatacg
     5641 ctgaggggcg gtagtcgtgg tcatggtgtg tccgtgtttg gcagttttta tttgaatgcc
     5701 gtctgaaaat tcggtttcag gcgagtttga aaaggcgata atgtagttca atttttgata
     5761 tttttcaatg ccgaaacagg aaataccctt atagaaacaa aataacgctt tgagaaattg
     5821 ttgacaatat tatcaaatat aaattttagg tggtgtttcc ggcgtatgac cgtatcaaaa
     5881 cggttgagtt tttcagaaaa cccgttataa tgccgtttct ctgaggcggg tctccccgca
     5941 tggcaaatcg gaacaccggg tcaggggcgg aagccagcag cccactccga tgcgccagtg
     6001 ccgggggttt ggcccgccgc cctatttgaa acgccggagc tttgatgttc cggcgttccc
     6061 gtatcgcacc gatgccgtct gaaagccgtt cagacggcat tttctttact ttgccataac
     6121 gtataactac aatgtgaaaa caattccaat gtgcaatgca cctgccgaca attttaagga
     6181 aactgccatg agcgcatcac aattactgtc aagactgacc caaaccgtag gcgaaaaata
     6241 catcatcacc gaacccgcga aaaccgaaca ataccgccaa ggctaccgct tcggcgaggg
     6301 taaggcgttg gcggtggtgc gccccggaag cattctggaa atgtggaaaa ttctacaggc
     6361 gtgcgtcgaa gcggacgtga ttgtgattac gcaggcggcg aataccggtt tgacgggcgg
     6421 ctcgaccccc gacggcaacg attacgaccg cgacatcgtg atagtgaaca ctatgcggat
     6481 gaacatcatc caaaccatca acgacaacga acaagtcgtc tgcctgcccg gctcgaccct
     6541 gaaccagctt gaattgctgc tgaaaccttt ggggcgcgaa ccgcattcgg tcatcggctc
     6601 ttcctgcatc ggcgcgtccg tcttgggcgg cgtgtgcaac aactccggcg gcgcgttggt
     6661 acagcgcggc ccggcctaca ccgaaatggc gttgttcgcc caaatcaacg aagaaggcag
     6721 gttggaactg gtcaaccact tgggcataga cttgggcgac acgcccgaag aaatcctgac
     6781 caacctgcaa ggtcatcatt atcaaaacaa agacatcaaa caagacgcgg gcaaagggca
     6841 cgaccacgcc tattgcgaac acgtccgcca agtggacgaa ccgaccgccg cgcgtttcaa
     6901 tgccgacccc gcccgccatt acgaagcctc gggctgcgcg ggcaaactga tggtttttgc
     6961 cgtccgtttg gacaccttcc cgcaagaaaa acaaaccgcc gtgttctaca tcggcacgaa
     7021 cgacatcaac gagctgaccg acatccgccg cgccgccttg ggcgaatttg aaagcctgcc
     7081 tgtttccggc gaatacatcc accgccacgc tttcgacatt gccgacgtgt acggcaaaga
     7141 cacgttttac gtcatcaaaa aattcggtac gcaccaactg ccgaaattat ttgacttgaa
     7201 ggcgcgcgtg gacaggttcg gcaaaaaagc cagcttcctg cccaaacatt tttccgacaa
     7261 ggcaatgcag ttcgtcagca aatacctgcc cgaccacctg cccaaatcca tgcgcgatta
     7321 ccgcgacaaa tacgaacacc acctgattct gaaaatgggc gggaaaggcg tggatgaggc
     7381 gcgcgcgttc ttaaaagaat attttgcaca ccacagcggc gcgtttttcg agtgcaacgc
     7441 cgaagaaacc caagccgcga tgctgcaccg tttcgccgtc gcctccgccg ccatccgcta
     7501 ccgcgccgtg catgatgacg aagtggaaga tttggtcgcg ctcgacatcg ccctgcgccg
     7561 cgacgaccgc gattggtttg aaaaactgcc gccggaaatc gacaataaaa tcatccataa
     7621 attgtattac ggacatttca tgtgccacgt tttccatcag gattacatca tcaaaaaagg
     7681 caacgactgc atggcattgg aacacgaaat gctgcatctt ttagaccaac gcggcgcgca
     7741 atatcccgcc gaacacaacg tcggtcattt gtatgaagcc aaacccgcgc tcaaacagtt
     7801 ttaccgcaaa ctcgacccga ccaacagctt caacccgggc gtaggcaaaa ccagcaaaaa
     7861 gaaaaactgg gcggaataag cgcgtccgct ttgaaggcag gcaatgccgt ctgaaggcaa
     7921 aacgccgttc agacggcatt gtcgggtttg aagttccccg tcgggtaatc gctttcaaac
     7981 cgtatgccga cacacgggcg cgcccctccc aaatatagtg gattaaattt aaatcaggac
     8041 aaggcaacga agccgcagac agtacagata gtacggcaag gcgaggtaac gccgtactgg
     8101 tttaaattta atccactata aagtctgatc ctaccttccc aaagggcagg gtttcaaccg
     8161 aaaaggaaac acgatgaaac catacaaaat ctacacccat cccgccctgc cgccccaagc
     8221 cgtcaaacaa ggctggtcat ggccggggct cttgttcggc acgctgtggg cgtgtttcaa
     8281 aagaatgtgg ggtttggggc tggggctgac gggcgcgata ttcgtgctgg ctgtgtttgc
     8341 ccaactggtt tacggcgaca cgcccgccac agactcggcg tttaacgtat tgggcttggc
     8401 ggtttccgtc tggttcggcg caaagggcaa cagcctttat gcacgccacc ttttatcgcg
     8461 cggctatacc gaattgcccg aaacggttga ggcagccaat ccccaagccg cattggcgca
     8521 atatttcggg cgcgggggcc gataagcgcg ttttcttttc catcatgccg tctgaaaaat
     8581 gttcagacgg catttcttat aaccttttgt ttgcagcaca taaccaatca gtctttccta
     8641 ttccgttccg aaatgcatac catgcgcccg tacccaatat ttgcgaacaa ggaaagaaaa
     8701 tggcacgttt aaccgtacac accctcgaaa ccgcccccga agccgccaaa gcgcgcgtcg
     8761 aggcggtact tcaaaacaac ggctttatcc ccaaccttat cggcgtatta tcaaacgccc
     8821 ccgaagcctt ggcgttttac caagaagtcg gcaagctcaa cgccgccaac agcctgaccg
     8881 ccggcgaagt cgaagtcatc cagatcatcg ccgcccgtac caaccaatgc ggtttttgcg
     8941 tggcagggca caccaaactc gcaaccctga aaaaactcct gtccgaacaa tccgtcaaag
     9001 ccgcgcgcgc tttggcagca ggcgaatttg acgatgccaa actcggcgcg ctcgccgcct
     9061 tcacccaagc cgtaatggcg aaaaaaggcg cggtatccga cgaggaactc aaagcatttt
     9121 tcgatgcggg ctacaaccag cagcaggcag tcgaagtcgt gatgggcgta gccttggcaa
     9181 ctttgtgcaa ctacgtcaac aacctcggac aaaccgaaat caaccccgaa ttgcaggctt
     9241 acgcctgata cggcaaaccg cccgaatatc gggcggtttt tcaaaaacac ccctcaaaca
     9301 aaaacaagcc gcctaagcgg cagggaagca gccgcgcatc aggcgcgccc ccgatttccc
     9361 cgcaaacctt aaggaacaaa gatgaacgcc caaaccctga ttgccaacgt tgccgaattc
     9421 gtcaaaacca agctcaaacc catagtggac gacatcgacc gcaaaggata ctaccccgaa
     9481 gcatttatgc gcgaactcgg cgcaatcggt ggattcggcg cagtcggtac agaagccgaa
     9541 ggcggcaacg gcttgggttt ggcaacgcaa atcgccgtat tgcgcgaaat cggcaaagaa
     9601 tgcggcgcaa cctctttcag tgcgtggtgt caggcggctt gcgcgtggta tctgcaccaa
     9661 acgcccaacc gggccgtcaa agacaaatac cttgccgaca tcctgcaagg caaagtattg
     9721 gcgggtacgg gcatgtccaa taccgtcaaa caccttgccg acatcgaaaa acacaacctc
     9781 caagccgagc gcgtggaggg cggctacaca gtcaacggcg cgctgccgtg ggtgtccaac
     9841 atcggcgaag accacatttg ggcgaatacc gcccaaatcg gcggcggcta cgtcatgttc
     9901 atcacaggcg gacaatggga aggcgtaagc ctgcaaaact gccccgaatt ttgcgccctc
     9961 gaaggcacgc gcaccttcag cctgaacttc aaagacgtat ttatccccga cgaagacatc
    10021 atcgccgcgc ccgaacagtt tgccgactac atccaaagca tcaaagcagg ctttatcctc
    10081 ctgcaaatcg gcatcggcgc gggcgtgata gacggcagcc tcggcatcat ccgcctcgcc
    10141 aacgtcgtca acgccgaagt caaccgctat ctcgacgacg gctacgacag cctcaaaaca
    10201 aggctggacg gcgcgtgggc agaaaccgaa cggctcgccg gattggcttg gagcggcacg
    10261 cccgacaacc ttaccaccct caagctgcgc gaagccgccg ccgtactgac acttgccgcc
    10321 gcccaatccg ccgccctgca ttccggcgca aaaggctacc tgatgcggag tcccgcccaa
    10381 aggcgcgtcc gcgaagcaat gtttgtcgcc atcgtaactc ccgcgatcaa acacctccgc
    10441 aaagaaatag cggcaatcga agccgcaaaa taaacaggcg gaaaaaaccg aaacccccat
    10501 cgcgtcattc ccgcgaaagc gggaatccag cccccaacgc aacaggaatc catcggaaaa
    10561 aacagaaacc ctcaaaccgt cattcccgca aaagcgggaa tccagccccc taacgcaaca
    10621 ggaatccatc gaaaaaaccg aaacctccaa accgtcattc ccacggaagt gggaatccag
    10681 taatcgaaaa accacaggaa tctatcgaaa aaaaacaacc ccaaaaaaac cgggcggata
    10741 ccgcaccatc ctcccaaacc ctgatttata gcggatgaac aaaaatcagg gcaaggcggc
    10801 gaagtcgcag acagtacaaa tagtacgggt gcttcagtac tttagagaat cgttctcttt
    10861 gagctaaggc gaggcaacgc cgtaccggtt tttgttaacc cactatacaa aaaacaagga
    10921 aacacaaatg gcgcaatata tgtgcggccc ctgcggctgg atttacgatg aagaactcgg
    10981 cgaccccgaa cacggcatcg cccccgggac aaagtttgaa gacatccccg acgactggaa
    11041 atgccccgaa tgcggcgtgg gcaaagaaga tttctacctg ttggatttcg tgatatagcg
    11101 gcagccgcac ccttagggaa aaccccgtca tccggcgttc cgcctgcggc ggcagggaac
    11161 accgttttat agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacaa
    11221 atagtacgga accgattcac ttggtgcttc agcaccttag agaatcattc tctttgagct
    11281 aaggcgagcc aacgatgtac tggtttttgt taatccacta tatccgtttg cgcccgccaa
    11341 gccgtgttgc acagatgcga cacggctttt ttctttctgt tgtcagaatg taaattttcg
    11401 tcaaaaaaca tcagaaatca cccccccccg aatttgcgta aaataaagtg taatcccttt
    11461 gatcataagg gaatgttgac aattagatcg attctcctag gctgactttc gcaataaatg
    11521 gggttgtcaa taattgtgta tggtcgtttg ccgtccggcg gccgtcattc ccgcgcaggc
    11581 gggaatccag caacacaatg cagcaggact ttatcggaaa aaaccgaaac cccaccgacc
    11641 gtcattcccg caaaagctgg aatccaaaaa cgcaacgcaa caggaattta tcggaaaaaa
    11701 cagaaacccc ccaccgccgt cattcccgca aaagcgggaa tccagcaacc gaaaaaccac
    11761 aggaatctat cagcaaaaac agaaacccca ccgccgtcat tcccgcgaaa gcgggaatcc
    11821 agacccgtcg gcacggaaac ttaccggata aaacagtttc cttagattcc acgtcctaga
    11881 ttcccgcttt cgcgggaatg acgagatttt agattatggg aatttatcag gaatgattca
    11941 atccatagaa aaaccacagg aatctatcag caaaaacaga aacccccacc gcgtcattcc
    12001 cgcaaaagcg ggaatccaga aacacaacgc ggcaggactt tatcggaaaa aacagaaacc
    12061 ccaccgaccg tcattcccgc aaaagcggga atctagaaac acaacgcggc aggactttat
    12121 cagaaaaaac agaaacccca ccgccgtcat tcccgcaaaa gcgggaatct agacccgtcg
    12181 gcacggaatc ttaccggata aaacagtttc cttagattcc acgtcctaga ttcccgcctt
    12241 cgcgggaatg acgagatttt agattatggg aatttatcag gaatgattga atccatagaa
    12301 aaaccacagg aatctatcag aaaaaacaga aacccccacc gcgtcattcc cgcgcaggcg
    12361 ggaatccagc aacacaacgc ggcaggactt tatcggaaaa aacagaaacc ccaccgaccg
    12421 tcattcccgc aaaagctgga atccaaaaac gcaacgcaac aggaatttat cggaaaaaac
    12481 agaaacccca ccgccgtcat tcccgcgaac gcgggaatcc agacccgtcg gcacggaatc
    12541 ttaccggata aaacagtttc cttagattcc acgtcctaga ttcccgcctt cgcgggaatg
    12601 acgagatttt aagttggggg aatttatcag aaaaccccca acccccaaaa acagggcgga
    12661 tgccgcacca tccgccccca aacccctttt aaccattcaa acaaacagaa agaaaaaaca
    12721 aaatgaacaa aatataccgc atcatttgga atagtgccct caatgcctgg gtcgtcgtat
    12781 ccgagctcac acgcaaccac accaaacgcg cctccgcaac cgtggcgacc gccgtattgg
    12841 cgacgctgtt gtctgcaacg gttcaggcga gtactaccga tgaagacgat gaagagttag
    12901 aacccgtagt acgcactgct cccgtgttga gcttccattc cgataaagaa ggcacgggag
    12961 aaaaagaagt tacagaagat tcaaattggg gaatatattt cgacaataaa ggagtactaa
    13021 aagccggagc aatcaccctc aaagccggcg acaacctgaa aatcaaacaa aacaccaatg
    13081 aaaacaccaa tgacagtagc ttcacctact cgctgaaaaa agacctcaca gacctgacca
    13141 gtgttgaaac tgaaaaatta tcgtttggcg caaacggcaa taaagtcaac atcacaagcg
    13201 acaccaaagg cttgaatttc gcgaaagaaa cggctgggac gaacggcgac accacggttc
    13261 atctgaacgg tatcggttcg actttgaccg atacgctgct gaataccgga gcgaccacaa
    13321 acgtaaccaa cgacaacgtt accgatgacg agaaaaaacg tgcggcaagc gttaaagacg
    13381 tattaaacgc aggctggaac attaaaggcg ttaaacccgg tacagcttcc gataacgttg
    13441 atttcgtccg cacttacgac acagtcgaat tcttgagcgc agatacgaaa acaacgactg
    13501 ttaatgtgga aagcaaagac aacggcaaga gaaccgaagt taaaatcggt gcgaagactt
    13561 ctgttatcaa agaaaaagac ggtaagttgg ttactggtaa aggcaaaggc gagaatggtt
    13621 cttctacaga cgaaggcgaa ggcttagtga ctgcaaaaga agtgattgat gcagtaaaca
    13681 aggctggttg gagaatgaaa acaacaaccg ctaatggtca aacaggtcaa gctgacaagt
    13741 ttgaaaccgt tacatcaggc acaaaagtaa cctttgctag tggtaatggt acaactgcga
    13801 ctgtaagtaa agatgatcaa ggcaacatca ctgttaagta tgatgtaaat gtcggcgatg
    13861 ccctaaacgt caatcagctg caaaacagcg gttggaattt ggattccaaa gcggttgcag
    13921 gttcttcggg caaagtcatc agcggcaatg tttcgccgag caagggaaag atggatgaaa
    13981 ccgtcaacat taatgccggc aacaacatcg agattacccg caacggtaaa aatatcgaca
    14041 tcgccacttc gatgaccccg cagttttcca gcgtttcgct cggcgcgggg gcggatgcgc
    14101 ccactttgag cgtggatggg gacgcattga atgtcggcag caagaaggac aacaaacccg
    14161 tccgcattac caatgtcgcc ccgggcgtta aagaggggga tgttacaaac gtcgcacaac
    14221 ttaaaggcgt ggcgcaaaac ttgaacaacc gcatcgacaa tgtggacggc aacgcgcgtg
    14281 cgggcatcgc ccaagcgatt gcaaccgcag gtctggttca ggcgtatctg cccggcaaga
    14341 gtatgatggc gatcggcggc ggcacttatc gcggcgaagc cggttacgcc atcggctact
    14401 ccagtatttc cgacggcgga aattggatta tcaaaggcac ggcttccggc aattcgcgcg
    14461 gccatttcgg tgcttccgca tctgtcggtt atcagtggta agggcttttt cgcctgtctg
    14521 ctgttggggc aggcggaagg tttgaaggga agggtggcga tttgccgcct gagacctttg
    14581 caaaattccc caaaatcccc taaattccca ccaagacatt taggggattt ctcatgagca
    14641 ccttcttccg gcaaaccgca caagccatga ttgccaaaca catcgaccgc ttcccgctat
    14701 tgaagttgga tcaagtgatt gactggcaac cgatcgaaca atacctgaat cgtcaaagaa
    14761 cccgttacct tagagaccat cgtggccgtc ccgcctatcc cctgttgtcc atgttcaaag
    14821 ccgtcctgc
//
LOCUS       DAWFJR010000044        14568 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWFJR010000044 DAWFJR010000000
VERSION     DAWFJR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14568
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.044"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(1224..1559)
                     /locus_tag="WGR04_001225"
     CDS             complement(1224..1559)
                     /locus_tag="WGR04_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ0794778.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFAQGL
                     PPGVGDFFR"
     gene            complement(1639..3771)
                     /gene="dnaX"
                     /locus_tag="WGR04_001226"
     CDS             complement(1639..3771)
                     /gene="dnaX"
                     /locus_tag="WGR04_001226"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ0794779.1"
                     /translation="MAYQVLARKWRPKTFADLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKITYAPPALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPNALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAATSCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPEAETAQTSVQTASAAAMPSEGKTAEPVTNQENNDVPPWEDAPDEAQTAAGTT
                     AQTSAKSIQTASEAETPPENQVSKNKAADNVTDAPLSEVPSENPIQATPNDEAVETET
                     FAHEAPAEPFYGYGFPDNDCPVEDGAEIPPPDWEHAAPADTAGGGADEEAEAGGIGGN
                     NTPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARA
                     TADKKRLDKIRDTLAQAYGLQLTLQTQDWRDEAGRETPAMQDKRVQAEDRQKAQALLE
                     ADPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    complement(2543..2582)
                     /gene="dnaX"
                     /locus_tag="WGR04_001226"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            complement(3866..5842)
                     /gene="mutL"
                     /locus_tag="WGR04_001227"
     CDS             complement(3866..5842)
                     /gene="mutL"
                     /locus_tag="WGR04_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ0794780.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAIE
                     VELAGGGIRLIRVSDNGGGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRADLTE
                     SVSNAGEVLHDITGVTPAPMPSENDSENLFDSASDYPTGNKPDTRNAFGSSGKTAPMP
                     YQAARAPQQHSLSLRESRAAMNTYAELYKKTDDIDLELSRLEQARFGNMPSETPIPKT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGKLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAVPAML
                     GKADVVSLAKDVLGELAQVGSSQTIEEHENHILATMSCHGSVRAGRQLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            5986..6171
                     /locus_tag="WGR04_001228"
     CDS             5986..6171
                     /locus_tag="WGR04_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ0794781.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            6182..6850
                     /locus_tag="WGR04_001229"
     CDS             6182..6850
                     /locus_tag="WGR04_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ0794782.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKTGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTALALALPKHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPRYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            6850..7566
                     /locus_tag="WGR04_001230"
     CDS             6850..7566
                     /locus_tag="WGR04_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ0794783.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRPEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGRIVRLSNEVETLNGKVKALEHAKTHPSGRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTAETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAIFKIGECQYRLQQKDIARATWRSL
                     IQAYPSSPAAKRAAAAVRKR"
     gene            7614..8099
                     /gene="purE"
                     /locus_tag="WGR04_001231"
     CDS             7614..8099
                     /gene="purE"
                     /locus_tag="WGR04_001231"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ0794784.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANENPELAQKLADFRAKQEQTVLNMELEQ
                     I"
     gene            complement(8282..9736)
                     /locus_tag="WGR04_001232"
     CDS             complement(8282..9736)
                     /locus_tag="WGR04_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ0794785.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPTEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(9733..10434)
                     /locus_tag="WGR04_001233"
     CDS             complement(9733..10434)
                     /locus_tag="WGR04_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ0794786.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEVERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            complement(10431..11210)
                     /locus_tag="WGR04_001234"
     CDS             complement(10431..11210)
                     /locus_tag="WGR04_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ0794787.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVARAQLDLFPEEWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPNMPRPKHIASFLLERTGGKA"
     gene            11494..12900
                     /locus_tag="WGR04_001235"
     CDS             11494..12900
                     /locus_tag="WGR04_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0794788.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADM
                     NESPLDMQLAVVAYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTPS"
     gene            13012..14538
                     /locus_tag="WGR04_001236"
     CDS             13012..14538
                     /locus_tag="WGR04_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ0794789.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVRTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
BASE COUNT         2914 a         3898 c         4108 g         3648 t
ORIGIN      
        1 gcgttcgcgg gaatgacggg gtgtaagttt ctgctcccac ggggctggat tcccgttttc
       61 atgggaatga cgaaatttca gacggcattt aagcggtacg gatgtgtaaa taatggtagg
      121 gtgggcttca gcctgccgat tcccgctatt cttgccgttt ttgcgttctt atcattctca
      181 ctgtttttac cgttcacgcc gaatcctgca aattgatgcc gtcattcccg cgaaggcggg
      241 aatccagacc cgtcggcacg gaaatttatc gagtaaaacg gtttctcaga ttctacgttc
      301 tagattcccg cgttcgcggg aatgacggtc gggggtttcc gttttttccg atagattccc
      361 gtggtttttc ggttactaga ttcccgcgtt cgcgggaatg acggcggcgg ggggttctgt
      421 tttttccgat agattcctgt ggtttttcgg ttactggatt cccgcgttcg cgggaatgac
      481 ggggtgtaag tttctgctcc gacggggttg gattcccgct ttcacgggaa tgacgaaatt
      541 tcagacggca tcggaatttt tgtgttttgg tgggcttcag cctgccgcat cccatcgatt
      601 ctgccgtttt taccgtttcc gccgaatcct tcaaactgat gccgtcattc ccgcgaaggc
      661 gggaatctag acctgtcggc acggaaattt atcgagtaaa acggtttctc agattctacg
      721 ttctagattc ccgcgttcgc gggaatgacg gcggcggggg gttctgtttt ttccgataga
      781 ttcccgtggt ttttcggtta ctggattccc gcgttcgcgg gaatgacggc ggcgggggtt
      841 ctgttttttt ccgatagatt cccgtggttt ttcggttact ggattcccgc ttttgcggga
      901 atgacggggt gtaagtttct gctccgacgg ggttggattc ccgttttcat gggaatgacg
      961 aaatttcaga cggcatttaa gcggtacgga tgtgtaaata atggtagggt gggcttcagc
     1021 ctgccgattc ccgctattct tgccgttttt gcattcttat cattctcact gtttttaccg
     1081 ttcacgccga atcctgcaaa ttaatgccgt cattcccgcg aaggcgggaa tccagacccg
     1141 tcggcacgga aatttatcga gtaaaacggt ttcttagatt ctacgttcta gattcccgcc
     1201 tgcgtgggaa tgacggtcgg ggatcagcgg aagaagtcgc ccactccggg ggggagtcct
     1261 tgggcgaatg cgcccattgt tttgtttgcg gtttcttcgg ctttgccccg ggcggatttg
     1321 agggcggcga ggatgaggtc ttcgagcatt tctttgtcgt cggcggcttc ttgaatcaaa
     1381 tcggggctga tgtcgatttt gcgtacttcg tgcgcgcagg tcattgtgat tttgaccagg
     1441 ccgttgcctg cttcgccttc gatttcggtt tcggcaagtt tggcttgcgc ttttttcata
     1501 ttttcctgca tttgctgcgc ctgtttcatc aggccgccta atccggcttt tccgaacata
     1561 ctgaatactc ctgtctgttt tgattgaatg agggaaacgg caatgccgtc cgaagggttc
     1621 gggcggcatt atatctgttt atggccggtt tgctgccaat tccagtgatt cgggctgcca
     1681 ttgcgcgccg aatgcttgga ggattttttg tgcggcgggg tcggcttcga gcaatgcttg
     1741 tgctttttgc ctgtcttcgg cttggacgcg cttgtcctgc atcgcggggg tttccctgcc
     1801 ggcttcgtcg cgccagtctt gggtttgcag ggtgagttgc agtccgtagg cttgggcaag
     1861 ggtgtcgcgg attttgtcga gacgtttttt gtcggcggtg gcgcgggctt cggcggtcat
     1921 cgccaaaacc atcaggccgg tgtcgggatg gtattccgtc cacgcggagt gttgcgccgg
     1981 catttgcgcc gcgccgagtt tgcgggcgaa atgccggacg atggctgccc agttttcggt
     2041 ggaaaattcg ggcggcgggg cggacggcgt gttgtttccg ccgatgccgc ctgcttctgc
     2101 ttcttcgtcc gcgcctccgc ctgcggtatc ggcaggggcg gcgtgttccc aatcgggcgg
     2161 cgggatttct gcgccgtctt ctacggggca gtcattatcc ggaaagccgt aaccgtagaa
     2221 aggttctgca ggggcttcgt gtgcaaatgt ttccgtttca acggcttcat cattcggtgt
     2281 tgcctgaatg gggttttcag acggcacttc ggacaagggg gcatcggtta cgttgtcggc
     2341 tgccttgttc ttggaaactt ggttttcggg cggcgtttcg gcttcggatg ccgtctgaat
     2401 gctttttgcc gacgtttgcg cggtcgtgcc ggctgcggtt tgggcttcgt ccggcgcgtc
     2461 ttcccaaggc ggaacatcgt tgttttcttg attggtaacg ggttcggcag ttttgccttc
     2521 agacggcatt gccgctgcgg atgccgtctg aacggatgtt tgggcggttt ccgcttcagg
     2581 gcgcggttgg ggcttttttg cggcggtttc cttttcggcg gtttgtgccg atggggattg
     2641 aagttcggta ttttcaatca cggcatttgc atcacacgat gttgccgcca agggcgcaaa
     2701 cgccagcatc cgcagcaggg tcatcataaa gccggcgtat tcgtcggggg cgaggctcag
     2761 gtcgcgtttg ccgtggacgg cgatttggta gtaaagctgg atttgttcgc cgcttatggt
     2821 ttgggcgagg cggtgcaaaa tatcggaatc ggggtcgtcg tgcgccaagg cattcggcac
     2881 tgcctgtatc agggcgaggt gttgcagcag tatggcgagt tcgcccaagg cgttgtcaaa
     2941 gccgacggca cacgccgcca tttcctgcgc tttggcggtc agggctgcgc cgtcttggtt
     3001 gatgatgcct gtcagcagtt cgtaaaggta ttgtttgtca accgcaccga tcatttggcg
     3061 gacatcgttt tcggcaactt tgcccgaacc tagggcgatg gcttggtcga gcaggctcaa
     3121 ggcatcgcgc atcgatccgg cggcggcgcg tcccaaaagt tgcagggcgg ggggggcgta
     3181 ggtgattttt tcgctgtcga ggacgtgggc gaggtggtcg gcaacctgct gcgcggtcat
     3241 attgcgtaag acaaattgca ggcagcggct caagacggta acggggactt tgtgcggatc
     3301 ggtcgtggca aggatgaatt tgacgtgttc gggtggctct tccagcgttt tgagcatagc
     3361 gttgaacgcg cttttggaaa gcatatgcac ttcgtcgatg atatagactt tgtatttgcc
     3421 ggcggtcggc gcgtattggg cgttttccaa tacttcgcgg atgttgtcga tgcctgtgtt
     3481 ggaagcggca tcgatttcca ataaatctac atagcgtccg gcatcgattt gggtgcagct
     3541 ttggcacacg ccgcaaggtt cgccgtgttg cgcgttttcg cagttgaggc ttttggcaag
     3601 gatgcgggcg atggtggttt tgcccacgcc gcgcgtaccg gtcagcaggt aggcgtggtg
     3661 cagcctgcct tcgtccaagg cattttgcag ggctttgacg acgtgttcct gaccgactaa
     3721 gtcggcaaag gttttgggcc gccattttcg ggcgagaact tgataggcca tatttttctc
     3781 ttggtttcgg tcgtgatgtt tctgtcggtg cgtcggaatg ccgtctgaac ggcggcttcg
     3841 ggcggcgtat tctagcactt tcggcttact gtccgcgcaa gaacagtgcg tccaattctt
     3901 tcaaagtcag tttgacccaa gtcggcctgc cgtggttgca ctggttgctg cgcggcgtgt
     3961 tttccatatc gcgcaggagc gcattcattt caggcaaggt cagttggcgg ccggcgcgga
     4021 cggagccgtg gcaggacatg gtggcgagga tgtggttttc gtgttcttcg atggtttggc
     4081 tgctgccgac ttgggcgagt tcgcccaata catctttggc cagcgaaaca acgtcggctt
     4141 taccgagcat ggcgggaact gcgcggacgg cgagggtgtt gccgcccata tcggacaatt
     4201 ccaagccgaa gcctgccagc gtttcggcat aatcggcaag ggcggcgcat tcttcgtggg
     4261 acgcggcaaa ggttacggga ataagcaggc gttggctttg cagcttgccg ttttcctgcc
     4321 gttggcgttt cattttctcg tagttgacgc gttcggcggc agcgtgcata tcgatgagca
     4381 gcaggctgtc ttcggcttgg gcgaggatgt agatgcccaa taattgggca atggcgaagc
     4441 cgagcggcgg cagttcggat tgggacggga tgccgtctga aagcggcgta tctgtttttg
     4501 ggataggcgt ttcagacggc atattgccga aacgcgcctg ctctaatcgg cttaactcaa
     4561 ggtcgatgtc gtcggttttt ttgtaaagct cggcgtaagt attcattgcc gcgcggcttt
     4621 cgcgcaggga caggctgtgt tgttgcggcg cacgcgcggc ttggtaaggc atgggcgcgg
     4681 ttttgcctga tgaaccaaag gcattgcgtg tatcgggttt gttgcctgtc gggtagtcgg
     4741 atgcgctatc aaacagattt tcgctgtcgt tttcagacgg cattggggca ggcgtaacgc
     4801 cggtaatgtc atgcaacact tcgcctgcgt tgctgacgct ttcggtcaag tcggcgcgtg
     4861 tgtcggcaag ggctttgttg agcgtgtgga acacgagttg gtgcacctgc cggctgtcgc
     4921 ggaagcggat ttcggttttg gtcgggtgga cgttgacatc cacggcttcg ggcggcaggt
     4981 cgagaaagag gacgaaagca ggggtaaggg cgttgtgcaa tacgtcgcgg tatgcctgtt
     5041 tgacggcgtg gagcatcact ttgtcgcgca cgaagcgatg gttgacgaag cagtattgtt
     5101 tgtcggtttt gcctttggcg aaagtcggtt tggcaatcgc gccatagagc cgcagcgcgc
     5161 cgttgccgct gtcaatttcc aatgaggccg tttgaaaatc atcgccgaca atggcggcga
     5221 tacgttcatg caggctttgt gcaggaagtt tgaatacttg tttgccgtcg cgtttgagtg
     5281 agaaggcgat gtgcggatgc gccagcgcga ggcgttcgag catggtggcg cagtgggcgt
     5341 attcggtgtt ttcggacttg agaaacttgc gccgcgcggg ggtgttgaag aagagttcgg
     5401 cagcttcgat ggtggtgccg acggggtggg cggcggcggt ggggctgctg agtttgccgt
     5461 cttcggcttt gacttgggtt gcgtgcgaac tgtcttcttg acggctggtc agggtcaggc
     5521 ggctgacgga ggcgatgctt gccaagcctt cgccgcgaaa gcccatgctg gcaacgtgtt
     5581 ccaaatcgtt taaggttttg attttgctgg tagcgtgtcg gtggagcgca agctcgatgt
     5641 cgtcggggtg gatgccgccg ccgttgtcgc tgacgcgaat caggcggatg ccgccgcccg
     5701 ccagctcgac ttcaatcgcc gttgcgcctg catcgatact gttttcaacg atttctttca
     5761 aggcgttggc agggcgttcg accacttcgc cggcggcgat ttggttgaca agatggtcgg
     5821 gcagggcggc aatgcgtggc ataaggtagg cttccgtttc aaaaaacgct ttattctata
     5881 ataaaacccc ttatctttct gcccgtattc ttgataaaag ccgtttatcc cgtttggaaa
     5941 ataccagtat aatcacgccg tatcccgtaa aattggagcg caaagatgta ccactaccaa
     6001 tccgaagcca cacaattcct caaccgtctg attgaagaaa aacctgagtt ggaacagcag
     6061 cgtttggaaa accgggggct tttgtgggat gtcgaactca atcccgaaga acaggaaaac
     6121 ttcgaggcgg caaaagtagc gaaaaaacct tatacctatt accaagactg aacccgccga
     6181 aatgacgacc catctgtcca atgtcgcccc cgacctgcaa aactatttga acgccatcgg
     6241 cgaacccgaa catcccgttt tgacgcggct gcgtgagaag accgggcatc accgtatggg
     6301 caaaatggcg attgcgcgcg aacaggcggc ggttttggtt tggctggcaa agctgattcg
     6361 cgcggaaaaa tatctggaag tcggcgtgtt tactggatac agcagcaccg cgcttgcatt
     6421 ggcgttgcca aaacacgggc ggattaccgc ctgtgacatc aatgtaacct ttaccgatac
     6481 ggcgcgtcag gtttggaacg aggcaggcgt ggcacataaa atcagcctgc atctgcaacc
     6541 cgcattgctg acattggatg atttgattgc acagggtgaa gccggaagct acgatttggc
     6601 actgatagac gcagacaaac cgcccacgcc gcgatatttc gagcgttgcc tcaaactcgt
     6661 ccgtcaaggc ggcatcatcg ccatcgacaa tattttgctg aacggaaggg tgatgcgcga
     6721 agcggcttcc gatgcgccgc ccagcgtcgg catcctcaaa gatttcaatc aaaacctgcc
     6781 gaacgatacg cggattgtcc ccatcaccct gcccgtcggc gacggcttga ccctgcttct
     6841 gaaaaaataa tgaagaccaa attaccgctt tttatcattt ggctgtccgt atccgccgcc
     6901 tgttcttccc ctgtttcccg caatattcag gatatgcggc ccgaaccgca ggcagaggca
     6961 ggtagttcgg acgctattcc ctatcccgtt cccactctgc aagaccgttt ggactatctg
     7021 gaaggcagaa tcgtccggct gtcgaacgaa gtggaaacct taaacggcaa agtcaaagca
     7081 ctggagcatg cgaaaacaca cccttccggc agggcatacg tccaaaaact cgacgaccgc
     7141 aagttgaaag agcattacct caataccgaa ggcggcagcg catccgcaca taccgccgaa
     7201 accgcacaaa acctctacaa tcaggcactc aaacactata aaagcggcag gttttctgcc
     7261 gctgcctccc tgttgaaagg cgcggacgga ggcgacggcg gcagcatcgc gcaacgcagt
     7321 atgtacctgt tgctgcaaag cagggcgcgt atgggcaact gcgaatccgt catcgaaatc
     7381 ggagggcgtt acgccaaccg tttcaaagac agcccaaccg cgcccgaagc catattcaaa
     7441 atcggcgaat gccaatacag gttgcagcag aaagacattg caagggcgac ttggcgcagc
     7501 ctgatacagg cttacccgag cagcccggcg gcaaaacgcg ccgccgcagc cgtgcgcaaa
     7561 cgatagtttt tccatttttc cgctttttac cccatccgca ccaaggagga tttatgatac
     7621 aaatcggcat catcatgggc agcaacagcg attggcccgt tatgcggcag gcagcgcagt
     7681 ttcttgaaga gttcggcgta gaatatgagg cgcgcgttgt ttccgcacac cgcaccccgg
     7741 atttgatgtt ccaatacgcc gaaaccgcac gggcgcgcgg catcaaagcc atcatcgcgg
     7801 gcgcgggcgg tgcggcgcat ttacccggta tggttgccgc caagaccacc gtccccgttt
     7861 tgggcgtacc cgtccccagc aaatacctgc gcggcgaaga ttcgctttta tcgattgtac
     7921 aaatgcccaa aggcgtaccc gtcgccacat tcgccatcgg cgaggcaggt gcggcaaatg
     7981 ccgcattgtt cgccgtttcc atgctggcca acgaaaaccc cgaactggcg caaaaactgg
     8041 cagacttccg agccaaacaa gaacagactg ttttgaatat ggaattggaa cagatttaaa
     8101 acccgccgtc cgcgaatata tagtggatta aatttaaacc agtacggcgt tacctcgcct
     8161 tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
     8221 tatacaaatg ccgtctgaag ccctgtttcg ggtttcagac ggcatttttg caaacaactg
     8281 tttatgcctg ttctttttgg cgcatttttt ctgccatcag ttcgtgcagg gttttcttcg
     8341 ccggtttcat cggcacgcgg ttttgcgtcc aacccaactg tttgcgcggg gtcaggttgc
     8401 ggaacttggt ggctgcccaa ccgaaggcgc ggtaggtttt gctgccgctg aaaataccgt
     8461 tgaatgtgcg ccacgccatt tgttcgccga aggtatgcga tgcgccttgc cctcggatgg
     8521 ggtgcggcac ggtttcggtc ggcgaacgtt gcgcttcaac gcgcaaacgc tgcatttgtt
     8581 cggtaatcgg gatgcgtacc ggacaaactt ccacgcacgc gccgcacatg gtacaggcgg
     8641 ttggcaggtc gcgagtggca tccagaccca acaggtgagg ggaaataatc tcgccaatcg
     8701 ggccgggata ggttgtgccg tatgccgcgc cgccgatgcg ggtataaacc gggcaatggt
     8761 tcatacacgc gccgcaacgg atacattgca gggtgcggcg catttggtct tcggcataag
     8821 cctggctgcg gccgttgtcg agcagaacca agtgcatttc ttgcggaccg tctaattctt
     8881 cactgcggcg cgggccggta atcatattga aataggttgt gatgttttga ccgatggcgg
     8941 agcgcggcag cagactgtac aagggtggga tgtcggacaa tttcgccacc actttttcaa
     9001 tgccggtaat agcgatatgc acgggaggaa cggtggtact caagcgaccg ttgccctcgt
     9061 tttccaccag acacagcgta cctgtttcag caacggcaaa gtttacgcca ctcaaaccga
     9121 catcggcagt gctgtaaata tcgcgcagtg ctttacgggc gaagccggtc agttggtcta
     9181 catcgtctgt cagcggcgta ccgaggtttt ggtggaacag ttcgctaacc tgttctttgg
     9241 ttttgtggat agcaggcatc acgatatggg tcggtttttc acctgccatt tggacgatga
     9301 actcgcccaa gtcgctttct accgctttaa tgccttttgc ttcaagataa tggttcagct
     9361 cgatttcctc gctgaccatg gatttacctt tgaccatcag cttgccgttt ttggctgtga
     9421 tgatgtcgtg gataatttgg caggcttcgg tcggggtttc tgcccagtgc actttcacgc
     9481 ccagcttagt taggttttct tccagctgct ccagcagggc aggtaattta gacaatgaac
     9541 gctgacggac gtgttcgcac aaatcgcgca ggctttgcag ctcttcttcg tcggtcaaaa
     9601 cggctttgcg tttggtcatc agcatatcca tcgcggtacg caggcttttg cgcaaaggct
     9661 tgtcttgaag ggaaattgcg gcgttttgct tgaaagtttc cggcttcatg tgaaatttga
     9721 tggtttgcgt agtcatgcgt tttcctccaa atcggcaggg gaaatgtggt cgggcaggat
     9781 ggcgaggatg accaaatcgc gcgggccgtg cgcgccgtaa gcaagcgtca gttggatgtc
     9841 tgcggttttg gacgggccgg aaatcaggaa tacattggtc ggcataccgt tttccaccag
     9901 tttttcgcct tcgacggcat tatgaaactc gttgtacatc ttggacgtat cgaacaggca
     9961 gaaatgcacg ggaggaacga ggcttaaagt acgcggctct tcggggctgg aaaacagcat
    10021 cagcgtgccg gtgcgggcga tgccgcattg cgcgccgctg aagcccgcat cgatgttcgt
    10081 gaaaaactcg gttttccaag tatcgatttc gcgctcgaag gcaatcggtt cgatattgct
    10141 gtccgccaat gcggcacggg caatttgtcc gtgttcggtc gccaagggca gcaggatgtt
    10201 tttcaaaccc ttgccttctg ccgcttcgcg gaaaacctgc atccaattgc ttttcgtcac
    10261 ccaataaatt tcggttttga ccgcacgcat agcggcagcc caatgtttca gacgctcaac
    10321 ttcgctgccc caagaaacac ccatttcacg gtaataatca aaaaccgcag gttcttccat
    10381 cggcaatgcg tcggcttttt tcagttttgc caaaatattt tcgcgcgcgc tcatgctttg
    10441 cctccggtac gttccaacaa gaaggatgcg atatgtttcg gacgcggcat attcggttcg
    10501 tccttagcaa ttttgccgcc gatgttcatc atacagccgc agtccgcgct gatgatttcg
    10561 gttgcgccgg tttctttcag cgcggcgact ttgtcggtta ccatcgcgcc ggaaatatcg
    10621 gcttgtttga cggagaatgt gccgccgaag ccgcaacatt cgctttcgtg gtcgtggacg
    10681 atgcgttcga cgttttccat accgtcaatc agttgccagc ccgagagatg gacattcatt
    10741 tcgcggcggg cggcgcagga agtgtgaacg gcgactttga gcggttcgcc cttgtcttcg
    10801 ggtttgaaac cgatggcaag caggaaatgg gtaaactcga tgatgcggcc ggcgcaatcc
    10861 acagcccttt cctcgtactc gctgccttta aacagcgtcg gccaatggtg cttcatcata
    10921 ccgccgcacg aaccggacgg tacgacaatc ggccactcct cggggaaaag gtcgagttgc
    10981 gctctggcga catcaaaggc ttcggtcgga tggccggatg aataggcggg ctgaccgcag
    11041 cagctttgcg ccatcgggaa atggacgcgt atgccctgtt gctcgattag ggtaatggca
    11101 tccatgcctg cttcgggcat aaaaagatca aggacgcaag tgccgaaaaa atagacatcg
    11161 gtcggattgc tgtcgtatcg gatgattttt gggggaatgg ttgcgctcat gttttttgat
    11221 aacgggaaac ccgttttttt ctgtagaaag gtaagcgttt actttaagta attgactgtt
    11281 gcgggtcaag tctaatttta aaaaataatc cggtttttct tacaaactac cccataacgc
    11341 ttactgtacc ttaatctgat agttttcgat aataattatc attacaatgc aatgccggtt
    11401 cgtttgcttg tgaacattca agatgccgac tctgacggca ttcagacagc atctgaaaac
    11461 aataacggca caaggatggg gtacaatcgc tctatggaaa aaaacaccct atccgcccgc
    11521 gcaccatccc cttggctgcc gcttctgctg gcaattgcca tttttatgca gatgttggat
    11581 gcgaccattt taaataccgc actgcctgaa attgccgccg acatgaatga gtcgcctctg
    11641 gatatgcaac tggcagtcgt tgcctatacg ctgacggttg ccctgctgat tcctttgagc
    11701 ggttatttgg cggacaggtt cggaacgaaa aaagtctttt tcggttcgat tgccgttttt
    11761 atgctcggat cggcattgtg cgccgcatcg ggttcgctgt ttgaattgac gctttcccgt
    11821 gtcgttcagg gcatcggcgg ttcgatgctg gttccgatac cgcgtctgac catcttgcgt
    11881 gtgtacgaca agtccaagct gctcaacgcc atcaattatg cggttatgcc cgcattaatc
    11941 ggaccggttt tagggccttt ggcgggcggt tatctggtcg aatacgcttc gtggcactgg
    12001 attttcctgc tcaacctgcc catcggtctt ttgggtttca tattgggacg caacatcatg
    12061 cccgatatta aaggcagcaa tatctcttta gacttcaaag gttatctgat tttttctgcc
    12121 gccgcgtgcc tcttgttact ttcggcagaa agcctgtcgc acgcgctgcc tccgtatttt
    12181 gcactgttgc cgctgtgcgg cggactgctg tttgcacgcc gttatttccg acatatgaaa
    12241 accgcgtcca aaccgattta ttccgccgac ctgtttctga tacgcacttt ccgtctagga
    12301 ctggcgggca atctgttcag ccgtctcggc atcagctcga ttccttttct gatgcccctg
    12361 atgtttcaag tcgctttcgg cttcggcgca agcctgtcgg gttggctggt cgcccccgtc
    12421 gccctgtctt cgctgctggt caaaccgctg attgcaccgc tcatgaaacg tttcggctac
    12481 cgcacggtac tgctttggaa caccaagctg cttgccgcct tcatcatgct gctcgccctg
    12541 cctgacggaa actcgccgct gtggatttgg attttcctct cgctggcgat cggcgcgtgc
    12601 aactccctac agttttctgc catgaacaca ctgaccctcg ccgatttgcg cccgcaacag
    12661 accggcagcg gcaacagcct gatggcggtc aaccaacagc ttgccatcag catgggcatt
    12721 gttgtcggcg cattaatcct taaaaactgg acatttctga taccggcttc ttcaggtctg
    12781 cattccgcct tccgtatgac cctgctcagc atcggcggca tcacccttgc ctcgtcgttg
    12841 gttttcaagc gtctgcacgt ttcagacggc gcaaacctga cacggaacac accgtcctaa
    12901 agcggtccgc acgcaaaact tttacccgtt tcaacgtttg gattatgatg ccgcacttcc
    12961 atgcgcgcca accccaaaac acaggcaatg ccgtctgaaa ccatatccct gatgaaaaca
    13021 cgcagcctaa tttccctttt atgcctcctt ctctgttcat gttcttcatg gttgccccca
    13081 ctggaagaac ggacggaaag ccgtcatttc aatacttcca aacccgtccg cctggacaac
    13141 atcctgcaaa tccggcacac ccctcatacc aacgggctat ccgatatcta tctgttgaac
    13201 gacccccacg aagcctttgc cgcccgcgct gcccttattg aatctgccga acacagcctc
    13261 gatttgcaat actacatctg gcgcaacgac atttccggcc gactgctgtt caacctcgtg
    13321 taccttgccg cagaacgcgg tgtgcgcgta cgcctgctgt tggacgacaa caacacgcgc
    13381 ggattggacg acctcctgct tgccctcgac agccatccca atatcgaagt gcgcctgttc
    13441 aaccccttcg tcttacgaaa atggcgcgca ctcggctacc tgaccgactt cccccgcctc
    13501 aaccgccgca tgcacaacaa atcctttacc gccgacaacc gcgccaccat actcggcgga
    13561 cgcaatatcg gcgacgaata cttcaaagtc ggtgaggaca ccgttttcgc cgacctggac
    13621 atcctcgcca ccggcagcgt cgtcggcgaa gtatcgcacg acttcgaccg ctactgggca
    13681 agccattccg cccacaacgc cacgcgcatc atccgcagcg gcaacatcgg caagggtctt
    13741 caagcactcg gatacaacga cgaaacgtcc agacacgcgc tcctgcgcta ccgcgaaacc
    13801 gtcgaacagt cgcccctcta ccaaaaaata caggcaggac gcatcgactg gcagagcgtc
    13861 cgaacccgcc tcatcagcga cgaccctgca aaaggactcg accgcgaccg ccgcaaaccg
    13921 ccgattgccg ggcggctgca agacgcgctc aaacagcccg aaaaaagcgt ctatctggtt
    13981 tcaccctatt tcgtccccac aaaatccggc acagacgcac tggcaaaact ggtgcaggac
    14041 ggcatagacg ttaccgttct gaccaactcg ctacaggcga ccgacgttgc cgccgtccat
    14101 tccggctatg tcaaataccg aaaaccgctg ctcaaagccg gcatcaaact ctacgagctg
    14161 caacccaacc atgccgtccc cgccacaaaa gacaaaggcc tgaccggcag ctccgtaacc
    14221 agcctgcacg ccaaaacctt cattgtggac ggcaaacgca tcttcatcgg ttcgttcaac
    14281 ctcgaccccc gttccgcgcg tctcaacacc gaaatgggcg ttgttatcga aagccccaaa
    14341 atcgcagaac agatggagcg cacccttgcc gataccaccc ccgaatacgc ctaccgcgtt
    14401 accctcgaca ggcacaaccg cctgcaatgg cacgatcccg ccacccgaaa aacctacccg
    14461 aacgaacccg aagccaaact ttggaaacgc atcgccgcaa aaatcctatc cctgctgccc
    14521 atagaaagtt tattatagaa atatagcgga ttaacaaaaa ccagtacg
//
LOCUS       DAWFJR010000045        13860 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWFJR010000045 DAWFJR010000000
VERSION     DAWFJR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13860
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.045"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(228..551)
                     /locus_tag="WGR04_001237"
     CDS             complement(228..551)
                     /locus_tag="WGR04_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ0794790.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(630..1286)
                     /locus_tag="WGR04_001238"
     CDS             complement(630..1286)
                     /locus_tag="WGR04_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ0794791.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPDVL
                     KQQLKDGGRMAVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFDF
                     "
     gene            complement(1388..1891)
                     /locus_tag="WGR04_001239"
     CDS             complement(1388..1891)
                     /locus_tag="WGR04_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ0794792.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAA"
     gene            2075..2848
                     /locus_tag="WGR04_001240"
     CDS             2075..2848
                     /locus_tag="WGR04_001240"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ0794793.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2855..3205
                     /gene="secG"
                     /locus_tag="WGR04_001241"
     CDS             2855..3205
                     /gene="secG"
                     /locus_tag="WGR04_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ0794794.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3219..3304
                     /locus_tag="WGR04_001242"
     tRNA            3219..3304
                     /locus_tag="WGR04_001242"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3253..3255,aa:Leu,seq:gag)
     gene            4106..4474
                     /locus_tag="WGR04_001243"
     CDS             4106..4474
                     /locus_tag="WGR04_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ0794795.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIEL"
     gene            4471..4782
                     /locus_tag="WGR04_001244"
     CDS             4471..4782
                     /locus_tag="WGR04_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ0794796.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            4892..5102
                     /locus_tag="WGR04_001245"
                     /pseudo
     CDS             4892..5102
                     /locus_tag="WGR04_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5163..6044)
                     /locus_tag="WGR04_001246"
     CDS             complement(5163..6044)
                     /locus_tag="WGR04_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0794797.1"
                     /translation="MKFNFLRSKPKYEISFLPSFRQIVCLSAVISILGGCAVVADVYG
                     HDSATMNAAAAKDYMKTVELNKSAGNVDTTSRTARRVQAVFRRMLPYADAANNTGHRF
                     DWKMTVFKNDELNAWAMPGGKMAFYTGIVDKLKLTDDEIAAIMGHEMTHALHEHGKNK
                     VGQQILTNTAAQIGTQTILDKKPDTNPELVGLGMDILGTYGLTLPYSRSLEEEADEGG
                     MMLMAQAGYHPAAAVRVWEKMNQENDQNGFIYAITSTHPTNNARIENLKRLLPTVMPV
                     YEHSVRNKGRVNKNRRR"
     gene            complement(6114..6245)
                     /locus_tag="WGR04_001247"
     CDS             complement(6114..6245)
                     /locus_tag="WGR04_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HEZ0794798.1"
                     /translation="MRDFDKPAEEAAEHAAEGAVKFIDGKPAKKIIAVPGRLANIVV"
     gene            complement(6375..7166)
                     /locus_tag="WGR04_001248"
     CDS             complement(6375..7166)
                     /locus_tag="WGR04_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0794799.1"
                     /translation="MNLFFDTELGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMKR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPHHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYEFENKLSIQ
                     FPQNNHIKDKIRQQLQILRDQNTIEFIGRGLYKKIDKLRPTLKAF"
     gene            complement(7395..10025)
                     /locus_tag="WGR04_001249"
     CDS             complement(7395..10025)
                     /locus_tag="WGR04_001249"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ0794800.1"
                     /translation="MQEHYRPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKRLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWEREVATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYARFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFATKYNLLKKQVIAVGDNAFDANQWQEWYGDKENGV
                     LVNSGDLDGMNFQTAFNAIAAKLQSQDAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     KCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETTCPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAEAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVGEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGEAVKAFAGSQDGLSKELKDLRHKLHSTIAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGGEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPAVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            10292..10807
                     /locus_tag="WGR04_001250"
     CDS             10292..10807
                     /locus_tag="WGR04_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0794801.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNNRGTFPTYDELDLMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            10814..11257
                     /locus_tag="WGR04_001251"
     CDS             10814..11257
                     /locus_tag="WGR04_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ0794802.1"
                     /translation="MPPRLLLGILCCLPIFAPAHAQGQPDTVGDFIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            11426..13483
                     /gene="ppk1"
                     /locus_tag="WGR04_001252"
     CDS             11426..13483
                     /gene="ppk1"
                     /locus_tag="WGR04_001252"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ0794803.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKREHKRCPQRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELAQEGIHFYRRRNWTGAQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRKSPILLHHPYQSFDPVVEMMREAAADSDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            13716..>13860
                     /locus_tag="WGR04_001253"
     CDS             13716..>13860
                     /locus_tag="WGR04_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0794804.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTVRSAADILGIHPNSAALFY
                     RKIR"
BASE COUNT         3409 a         3919 c         3409 g         3123 t
ORIGIN      
        1 cagcctgcac attgaaaatg ccgcccaagc aaactttcag tttgccagcc tcgtcctagc
       61 cctctcccac ggggagaggg gattggggtg caggctgcct ttaaggttca ggcaaatttt
      121 aacttcgttg ataccgcgct ttagcttcgc agaagctgca ctttcagacg accttttgga
      181 acaccacagg tacacgcatt taaggaatgc cgtctgaaat gcctgcctca ataacgcatc
      241 atgttgccgt caatctcggc cgcccatgca tcgatgccgc cctgaaggtt gtacaggttt
      301 tcaaaacccg cgtccgccaa atacatcgcc gtatgcaggc tgcggatacc gtggtggcaa
      361 tacaccacaa gcggcacatc atccggcagc tcgttctgcc gcagcggaat cagattcatc
      421 gggatatgca gcgcatttgg cagcgaacaa accgccgttt cttcatccgt acgcacgtcc
      481 aacaaacaaa acatccgccc ttcgtccatc cacgccttca attccgccga cccaagttgc
      541 acaatatcca tcgcaccacc aaaaaaaacc aagcaaaatg ccgtctgaag ccccaaatcc
      601 gctttcagac ggcatgacct gtcaacatct taaaaatcga aaccgccaaa cggatcggca
      661 tccttatcat ccaaatgcgc caccaaggta tcgaacagca ccttctcttc aaacacatcg
      721 cccttgcgcg taatcaaaag cgcgcgctga acaggcttgc ggcctacgat aaccgccatg
      781 cgtccgccat cttttaactg ttgtttcaac acatcaggca caaggtttac cgcaccgccg
      841 acataaaccg catcaaacgg cgcacctgcg gaaagttcgg tcaacccgtt gttttgcaca
      901 taatcgatat tgtccaaacc caagccgtcc aacaccgctt tggcgcggtt ttgctgttcg
      961 gcatcgatgt cgtccgacac cacacgacca gccaattttg ccaaaagcgc ggtcgcatag
     1021 cccgaacccg tgccgatttc caaaaccgta tcgtttttcg tcagcttcaa gccctgcgcc
     1081 agccgcgcca cgactttcgg ctccagcatc ttatgaccgt tggcaagcgg cagcgccata
     1141 tccgcatacg ccaaaccctg caaatcctca tcgacaaaaa gctcgcgcgg aatctccgcc
     1201 aaagcgtcca aaacgtcaaa atccaataca tcccacggac ggatttgctg ttcgaccata
     1261 ttgaaccgcg ctttttcaaa atccatttag tgctccgtca aataattgtt tcaacaatgg
     1321 caggcattat aaaaccacac tcccgccgcg ccaacttcgg cacgcgccgc ccgccccttt
     1381 ccatcactca agccgcctgg cgcgaagcct caaaccgctt ctccaaaatc ttcgccagtt
     1441 cgcccaaata caaagcatct gtctcatcaa actgcgccaa atgttcgctg tccgcgtcca
     1501 acacgccgat acagcggccg tctgaaaaca gcggcacgac aatctccgaa cgcgacaaag
     1561 acgaacaggc aatatggtcg ggatgcgcgt ccacatcccc gacgaccacc gtcccgccct
     1621 tcgcccaagc ctgaccgcac accccgcgac cgaacggaat ccgcgtacac gccaaaggcc
     1681 cctgaaacgg tgccaaaacc aattcgtccg aacgcgtatc gaccaaataa aaacccaccc
     1741 aaaaccaacc gaacgcctcc ttcaaaaccg ccgccgtgtt cgccaaattc gccacccaat
     1801 ccgtctcgtc cgccaccaca gactcaatct gcggcaacac ctcccgataa agcgcggcct
     1861 tgtccgaagc cgaaaaatga agcgcgtgca tcacatctcc tatagttgca tacatatcag
     1921 gcggccatta taaaacaacc tgcccgaaac aacattccaa accgcccccg cagccgcccg
     1981 ccccaagttg cgaacccgcc gcatatccac taaacttcac attgcaacgc gccacacgcg
     2041 gcagacagaa aaaacacgac acggagcaaa aaagatgtat caccaaatcg gaatgtggga
     2101 tcaaaaatgg gtcatcggca actggaaaat gaacggccgg ctccagaaca acaattcgct
     2161 catgcaccgc ttccgcatcc tccccaccgc cgaacgcgtc ctcatcggac tcgccgcccc
     2221 gaccgtttac ctgctgcaac tgcacaacgc catgcaaatc gtcttaaaca accgcatcct
     2281 cacctgcgcc caagacgtga gccgcttccc cgacaacggc gcgtacaccg gcgaagtgtc
     2341 cgccgaaatg ctcgccgaca ccggcacaga catcgtcctc atcggacact ccgaacgcag
     2401 cctttatttc ggcgaaaaaa acgaaatcca acgccgcaaa atggaaaacg tcctcaacgt
     2461 cggactcatc ccgttattgt gcgtcggcga aagcctcgaa gagcgcgaag ccggcaaaga
     2521 acacgaagtc atcgcccatc agctttccat cctgcaaggg ctggatacca aaaacatcgc
     2581 cgtcgcctac gaacccgtct gggcgatcgg caccggcaaa gtcgccaccg tcgaacagat
     2641 tgccgatatg cacgcattca tctacaaaga aatcttgtct ttgtgcggaa gcgatgttaa
     2701 aatccgcgtc ctttacggcg gaagtgtgaa agcggacaac gcggccgaca tcttcgcagt
     2761 accttatgtg gacggcgcac tcgtcggcgg cgcgtcattg tcgtacgact cctttaccgc
     2821 catcatcagt gccgcacaaa atgcgtagaa aaatatggaa gccttcaaaa ccctaatttg
     2881 gattgttaat ataatttccg ctttggccgt catcgtgtta gtattgctcc aacacggcaa
     2941 aggcgcggat gccggcgcga ctttcggatc gggaagcggc agcgcgcaag gcgtattcgg
     3001 ctctgccggc aacgctaact tcctcagccg ctcgaccgcc gttgcagcaa catttttctt
     3061 tgcaacctgc atggctatgg tgtatattca cacccacacg acaaaacacg gtttggactt
     3121 cagcaacgta caacaaactc agcaagcacc caaacccgta agcaataccg aaccttctgc
     3181 ccctgttcct cagcagcaga aataacagtt tttcaaatgc cgacatggtg aaattggtag
     3241 acacgctatc ttgagggggt agtggccgta ggctgtgcga gttcaaatct cgctgtcggc
     3301 accaacacac aaaacgctta gaaattctaa gcgttttttt tcgtttaatc ctgtctaatg
     3361 ccgtcaacca atcccaatca atacaaggat ttcaggctga ttgttatcct gccgtccccc
     3421 ttcctgacag tgcaatcccg tccaatccgc cctaattgaa ctaacctgat tttttacagc
     3481 atccgaaaat accgcgagaa aataccgtaa aaataaagct gaacgaccac caaatcagga
     3541 atgccaagcg ggaaatagct tgcggggaat actgacgcac cgtagggaat gagggggaaa
     3601 ccgtccaaga tgcagagcgg ttttttttgg gtttttggaa aaaacctata ctatagtgga
     3661 ttaaatttaa accggtacgg cgttgcctcg ccttgccgta ccggtttaaa tttaattcac
     3721 tataaaataa gaaaaagata aaaaattggt aacaaatgcg gtaacaaatg gtaacgaatc
     3781 ggtaacaact tttggggttt tccggttttt caccgtcttg gcagtgggag cgtagcggaa
     3841 tgaaaagcca aaacgcacgg aaccgcgcct attttgagca ggaatggcgg ttaaaccgct
     3901 tggttatata cggggcatag gaagacagcg aaacgcgcta aaaacgcaat ttgagccatt
     3961 aaaagcgatt gattaaaaaa taatcaggtt agccgccggt gtttcaggcg gcataaacgg
     4021 agaaattgcg gggcataaaa aaggcagctt gccgtgttgt ctgtctctgg tataattcca
     4081 agtatcacta atcaacggct acacaatgcg gatattcaaa aaccaatgga tagtgaaatt
     4141 tgccaagaag cacaaaatca acgattccga gctgctggaa gcggtagagc gggcggataa
     4201 cgggctgata gacgcagatt taggcggcgg tgtgattaag cagcgcatag caaggcaagg
     4261 acaaggcaga agcggcggct atcgcagcct gatattgttc aaacaggcag atagggcatt
     4321 tttcgtttac gcctttgcca agaacgacag ggaaaacatt tcggataaag aactggacgt
     4381 ttaccgaaaa gccgccgcat attatctgaa atacacgcgg gcagagctgg cggctttgaa
     4441 agaagacggc attatcacgg agatagaatt atgaaataca aaagcgaggc attagccgcc
     4501 attcatgaaa tgatggaagg ggcttacaac atcggcgcaa tcgacaaaaa gactatgcgc
     4561 ggctttgaca agtcctgcct gaccgaaatc aaaccgttga gcggcgggga catcaaggca
     4621 atcagggaga aggaggcact atcgcaagcc gctttcgcca tctatctcaa cgtgggaaaa
     4681 aatcacgttt cggcttggga gcggggcgtt aaaaagccga gcggcgcggc gttgaagctg
     4741 ctgaccatcg tcaaaaacaa gggtatcgaa gccattgcgt agccgacttg gcaaacggca
     4801 aaatcagcaa gttcacaata gacgcgctac tgaatatgcc tgccaagaca ggcaagaccg
     4861 ccgaaccgaa tatcagggca tagccgcaca aatgcccgac cgcatcaaac caagccgaaa
     4921 cggcggcggt gcagacggca tagcccgaca gcaaggcacg gcgcagacgg gcgcgaaacc
     4981 cgaaacatcc ccgaccgcga ggcacgggga ttttttgcgc ccgttgcagg gggattggat
     5041 ttaagcggcg cgggcttgaa ggcaaaacgg gcggggcgca gaactgttta aatgccgtct
     5101 gaatctcaaa cgatttcaga cggcattttg aaacaacggc tcaaattctc gactcccttc
     5161 ccttaacgcc gacggttttt attaacgcgc cccttatttc tgacactgtg ctcataaacc
     5221 ggcataacgg tcggcaacaa ccgttttaga ttttctatac gggcattgtt tgtcggatga
     5281 gtagaggtaa tagcataaat aaagccgttt tggtcgtttt cctgattcat tttttcccaa
     5341 accctgacag cggccgccgg atgatagcct gcctgcgcca tcaacatcat tcccccctca
     5401 tcggcttctt cttccaagct gcggctataa ggcaaggtaa gaccgtacgt ccccaaaata
     5461 tccataccca atccgaccaa ttccggatta gtgtccggtt ttttgtctaa tatagtctgc
     5521 gtgcctatct gcgccgccgt attggtcaaa atttgttgcc cgaccttatt tttaccgtgt
     5581 tcatgcaggg cgtgcgtcat ttcatgcccc ataatggcgg caatttcgtc atcggtcagc
     5641 ttgagtttgt cgactatccc cgtataaaac gccatttttc caccgggcat cgcccacgcg
     5701 ttcagctcat cgtttttgaa aaccgtcatt ttccagtcaa acctatggcc ggtattattt
     5761 gccgcatcgg cataaggcag catacgtcga aatactgcct gcaccctgcg ggctgttctg
     5821 gatgtggtat cgacattgcc ggcagacttg ttcaactcaa ccgttttcat ataatctttg
     5881 gcagccgcag cgttcattgt ggcagaatca tgaccgtaaa catcagcaac gaccgcacaa
     5941 cctcccagta tcgaaatgac tgccgatagg caaactatct gtctaaagga aggaaggaag
     6001 gaaatttcat atttaggttt actccttaaa aaattaaatt tcaaaaaatg ccgtctgaat
     6061 ccaaaacgga tttcggacgg catcttaaca ttgtttaatg tttttaaaaa gatttacacc
     6121 acgatgttcg ccagtctgcc cggtacggcg atgattttct tggcaggctt gccgtctatg
     6181 aatttcaccg cgccttcagc ggcgtgttcg gcagcttctt cagccggttt atcgaaatca
     6241 cgcataaatt gccaataatt ctccgacttt cttacgacta ctacctttgg cggcaatatt
     6301 gcgctgtact tcaactgttt tcagaatggc agaagaataa atatcccttg tgaattcagt
     6361 atcatgattt gaaatcaaaa cgccttgaga gtcggacgca atttgtcgat ttttttgtaa
     6421 agtccgcgac caatgaattc gatcgtattt tggtcgcgca aaatttgcaa ctgttggcgg
     6481 attttgtctt tgatatggtt gttttgggga aattggatgg atagtttgtt ttcaaattca
     6541 tacatttgcg acaatgtgaa ttcttcgggg agttggtcga tacatttcat aacagccaaa
     6601 agccagcctt tgcgctccgc attttggttg cgtaaaaaca ggttggattg ccattttttc
     6661 agaacggttt cgggttcgat aatgcgggaa tcgtctatta agaatatttt tccgctttca
     6721 ggcaaagggg cgagattgat ggaacacata atgtggtgcg gccggttttt aatgccttta
     6781 tttctaggaa taatcatatc cggcgtgatg aaatgtttgg gtacaagcac caattgccgt
     6841 atggagtaat ccgctttttt atatgcaaga aagaaaaagt tggggttggt atctgaccgg
     6901 atgcgctcca acatagtgcg atatgcaccg tcaggcacgc tgttgcctat ggttttttga
     6961 tttttactct ttaattcata ttgctcgtgg caatttgggc aaaagaggtc tgcaacaggt
     7021 ttgttattgg caaatctctt catcggcttg cttccgcaac aggggcagta gccgtttttt
     7081 tccagccaag cctcgctcat tacacggatt ttgtgggttg ctttattttg ttgctttccc
     7141 aattcagtat cgaaaaataa attcatgttt tggattttga gattaagttt tagtggatta
     7201 acaaaaatca ggacaaggag acgaagccgc agacagcagg gcaaggcggg gcaacgccgt
     7261 accggtttaa agttaatcca ctatatttga ggtctgtcat gcggacaacc caatctgcct
     7321 taaaaaggcc gtctgaaacc ctgtttccaa gtttcagacg gcctttatcc gtatggctaa
     7381 accttaaaag cggcttagac gacgatattc accaagcgtc cgggaacgac gatgattttc
     7441 ttcgcgggtt tgccttccat aaatttcacc gcgccttcgg tggcgagtgc ggcggcttcg
     7501 aggtcggctt tggatgcgtc ggcggcgacg gtgattttgc cgcgcagttt gccgttgact
     7561 tgaaccatga cttcgatttc ggatttgacc aaggcggctt cgtcgactgc cggccagcct
     7621 gcttcccaca gtttcgcgcc gttcaattcg ctccacaggg tttcgcagat gtgcggcacg
     7681 atgggccaca gcaggcgtac ggcggtttcc aatacttctt gggcgacggc gcggccttgt
     7741 tcgccgccgg tgtcggtttt gtcgtattgg ttgagcaatt ccatcacggc ggcgatggcg
     7801 gtgttgaact gctggcggcg gccgtagtcg tcgctgactt tggcgatggt agaatgcagt
     7861 ttgtggcgca ggtctttgag ttctttagac aaaccgtctt ggctgcctgc gaacgctttg
     7921 actgcttcgc cttgcttcaa gtattcgtaa acggtacgcc acaggcggcg caggaagcgg
     7981 tgtgcgcctt cgacgccgct gtcgctccat tcgagggact gttcgggcgg tgcggcgaac
     8041 atcatgaaca ggcgggcagt gtccgcgccg taggcgttaa tcagctcttg cggatcgacg
     8101 ccgttgtttt tggacttgga cattttttcc gtgccgctga tgacgacggg cagaccgtcg
     8161 gctttgagga cggcggaaac agggcggcct ttgtcgtcga acgtcagctc gacatcggcg
     8221 gggttgatcc aatctttgcc gcctttgtcg ttttcgcggt agtaggtttc gcaaacgacc
     8281 atgccttgcg tcagcaggcg ttcaaacggt tcgccaacat tgaccaagcc ttcgtcgcgc
     8341 atcagtttgg tgaagaaacg cgcgtacagg aggtgcaaaa tggcgtgttc gataccgccg
     8401 atgtattggt cgaccgcgcc ccagtatttc gcggcttctg ccgataccat gccgtctgaa
     8461 aacttgggcg acatatagcg gaagaagtac cagcttgatt ccatgaaggt gtccatggtg
     8521 tcggtttcgc gtttcgccgc gccaccgcag catgggcagg tggtttcgta aaactcgggc
     8581 atttttgcca aaggcgaacc catgccgtcg ggtacgacgt tttcaggcag gacgacgggc
     8641 agttggtcgg ctgggacggg tacgtcgccg catttttcac aatggacgat gggaatcggg
     8701 cagccccagt agcgttgacg ggaaataccc cagtcgcgca ggcggtattg ggttttcggt
     8761 tcgcccgcgt cttggctttg cagcttggcg gcgatagcgt tgaaggccgt ctgaaaattc
     8821 atgccgtcca agtcgccgct gttgaccaat acgccgtttt ctttgtcgcc gtaccattct
     8881 tgccattggt ttgcgtcgaa tgcgttgtca ccgacggcaa tgacttgttt tttcagaaga
     8941 ttgtatttgg tggcgaactc gaaatcgcgt tcgtcgtgcg ccggaacagc catcaccgcg
     9001 ccatcgccgt agccccacaa tacatagttg gcaatccaca cttccagctt gtcgccgttg
     9061 agcggattga cgacgtagcg gccggtcggc acgccttttt tctccatcgt cgccatatcg
     9121 gcttcggcaa ccgagccggc tttgcactcg gcgataaatg cctgcaattc gggtttgtcg
     9181 gcggcggctg cggttgccag cggatgctcg gcggcaacgg caacataagt cgcgcccatc
     9241 agcgtgtcgg ggcgggtggt ataaacttgc aggaatctcg cgtaatcgcc ttccaaacct
     9301 tgtttgctgt cgtctgaaac ggcaaagcgc acggtcatac cgcgagattt gccgatccag
     9361 ttgcgctgca tggttttgac ttgttccggc cagtgttcca gcttgtccaa atcgttgagc
     9421 aactcttcgg cgtaatccgt gattttgaag taatacatcg ggatttcgcg tttttcaatc
     9481 aacgcacccg aacgccagcc gcgcccgtcg atgacttgct cgttggccaa tacggtttgg
     9541 tcgactgggt cccagtttac cgtgccgttt ttgcgataca cgatgccttt ttcaaacagc
     9601 ttggtaaaca gccattgttc ccagcggtag tattcgggtt tgcaggtagc gacttcgcgc
     9661 tcccagtcaa tcgcaaaacc caggcttttg agctgggttt tcatgtattc gatgttgtcg
     9721 taagtccaag cggcgggggc gacgttgttt ttcatcgccg cattttccgc aggcataccg
     9781 aacgcgtccc aacccatagg ttgcatgacg ttgaagccgt ttaagcgttt gaagcggctc
     9841 aatacgtcgc cgatggtgta gttgcgcaca tgccccatgt gcagcttgcc gctggggtag
     9901 gggaacatgg agaggcagta gtatttgggt ttggaagcgt cttcggagac gttgaaaata
     9961 cgggcgtcgt cccatttttt ctgcgccgca ggctcgatgg cggcgggccg gtaatgttct
    10021 tgcatggtca ttctgttttt cgcttgaaaa cgttaggaat ttaaaggagg aattataaca
    10081 ggttgccgat gtcggcgaat cggcaaattg ccggcaggat gcgtaaattc gcacgcgcat
    10141 cattccgtat gccgtacaaa tacaccgcgt ttactgatac gcacgttttt tatgctaata
    10201 ttacaaacca aaatcaaatg tttaaaactc tcctgatgcg gctcttccga acaaaaggca
    10261 gacgggcatc gggtaaaaga ggattctgca tatgaaaatc aaacaaatcg tcaaaccggg
    10321 cttggcagta ttggcggcgg gcgttctgtc tgcctgcgca accaaaagca acgtcaaagc
    10381 cgacggaacg accgacaatc cggttttccc gaaaccctat tccgtaacgc tcgacaacaa
    10441 tcgcggtaca ttcccgacct atgacgaatt ggacttgatg cgtcccggtc tgaccaaaga
    10501 cgacatctac aaaatcctgg gtcgcccgca ttacgacgaa ggtatgtacg gcgtgcgcga
    10561 atgggattac ctgttccact tccatacccc gggcgtaggt atcgaccctg aaaacacttc
    10621 cggcgtagaa gatgttacta cctgccaata caaagtgatt ttcgataaag acaaatttgc
    10681 ccgcagcttc tactggaacc ccgtcttccc gaaagatgcc gcctgtccgc cgcccgcacc
    10741 caaagccgag ccgcaagtca tcatccgcga aatcgtgccg gcaaaaccga aacgtatccg
    10801 ccaataatac gcaatgccgc cccgcctgct tttagggata ttgtgctgcc tgccgatttt
    10861 tgcccccgcg cacgcgcagg ggcagccgga tacggtcggc gactttatcc aaaagaaaaa
    10921 agtcatcgtc gatacatcca aagcggaact ctgttttgcc gatgacaggc agtgccaccc
    10981 cgtcctcatc ggtgttgcca cgcccaaggg gacgttcggg ctgacgctga acagtaccga
    11041 caagcccgga tacggcggcg aagtcatcgg tttcaagcag gagggtgatt ttcttttcgc
    11101 cctgcaccgc gtttggaatc agataccgtc ggaaaggcgg aacgaacgca tcgcctcccc
    11161 gtccgtgtcc gacaggatta tgaccaacgg ctgcatcaac gtcagcgatg cggtgtacga
    11221 aaaactgcgt cattattttg tgttggaagt gatttgaaac agacggatac cgcacgcgcc
    11281 ggtatcggtt ttcacattgc cccgatgcct gaaacagact gtccgccacg tcatgccgtc
    11341 tgaaaccggc gcagatgccg ccaagccttc agacggcatt gcctgcccgc tccgaccgaa
    11401 caacaaccat ctttgggaga accttatgcc cgaacaaaac cgcatcctct gccgcgaact
    11461 aagcctgctg gcattcaacc gccgcgtgtt ggcacaggcg gaagacaaaa acgtccccct
    11521 tttggaacgc ctgcgcttcc tgtgcatcgt ttcatccaac ctcgacgagt ttttcgaagt
    11581 ccgtatggcg tggctgaagc gcgaacacaa acgctgcccg cagcgcaggc tggacaacgg
    11641 caaaatgccg tctgaaacca tcgccgacgt taccgaagcg gcgcgctccc tgatacggca
    11701 ccagtacgac ctgttcaaca acgtccttca gcccgagctg gcacaagaag gcatccattt
    11761 ttaccgccgc cgcaactgga cgggtgcgca gaaaaagtgg atcgaagact atttcgaccg
    11821 cgaattgttg ccgattctga cccccatcgg actcgaccct tcccacccct tcccgcgccc
    11881 gctgaacaaa tcgctcaact tcgccgtcga actcgacggc acagacgcgt tcggcaggcc
    11941 ttcgggtatg gcgattgtcc aagcaccgcg catcctgccg cgcgttgttc ccctgccgtc
    12001 cgaactgtgt ggcggcggac acggcttcgt cttcctctcc tccatcctgc acgcccacgt
    12061 cggcaaactc ttcccgggca tgaacgtcaa aggctgccac cagttccgcc tgacgcgcga
    12121 cagcgacttg accgttgacg aagaagacct gcaaaacctc cgcgccgcca ttcaaaacga
    12181 actgcacgac cgcgaatacg gcgacggcgt gcggctcgaa gtcgccgaca cctgtcccgc
    12241 ctacatccgc gactttctgc tcgcgcaatt caaactgacc gccgccgaac tctatcaggt
    12301 caaaggcccg gtcaacctcg tgcgcctcaa cgccgtcccc gacctagtca accgccccga
    12361 tttgaaattt cccacacaca cgccgggcag actgaaagcc ttgggcaaaa ccgcgtccat
    12421 attcgatttg gtgcgcaaat cgcccatcct gctgcaccac ccctaccaat cgttcgaccc
    12481 cgttgtcgaa atgatgcgcg aagctgccgc cgactccgac gtgcttgccg tcaaaatgac
    12541 gatttaccgc accggcacgc gttccgaact cgtccgcgcc ctgatgaagg cggcactcgc
    12601 cggcaaacaa gtaaccgtcg tcgtcgaact gatggcgcgt tttgacgaag ccaacaacgt
    12661 caactgggcg aagcagctcg aagaggcggg cgcgcacgtc gtgtacggcg tgttcggcta
    12721 caaagtccac gccaagatgg cactggtcat ccgccgcgaa gacggcgtac tcaagcgtta
    12781 cgcccacctc ggcacgggta actaccacca aggcacatcg cgcatctaca ccgacttcgg
    12841 catcattacc gccgacgaac aaatcaccgc cgatgtgaac acattgttta tggaaatcac
    12901 aggcttgggc aaacccgggc ggctgaacaa actctaccaa agcccgttta ccctgcacaa
    12961 aatggttatc gaccgcatcg cacgcgaaac cgaacacgca aaagccggca aaccggcgcg
    13021 gattaccgcc aagatgaatt cgctcatcga accgaccgtc atcgaagccc tgtatcgggc
    13081 aagcgcggca ggcgtacaaa tcgatttgat tgtgcgcggt atgtgcacct tgcgcccggg
    13141 tgtaaaaggc ttgtccgaaa acatccgcgt ccgctccatc atcggcaggc agctcgaaca
    13201 cgcgcgcgtg tattgcttcc acaacaacgg cgcggacgat acctttatct ccagcgcgga
    13261 ttggatgggg cgcaacttct tccgccgcat cgaaaccgcc gcgccgatta ccgcgcccga
    13321 actcaaaaaa cgcgtcatcc gcgaaggttt ggaaatggca ttggcagaca atacgcacgc
    13381 gtggctgatg cagcccgacg gcggctatat ccgcgccgca cctgccgagg gcgaatccga
    13441 agccgacctg caaaacgatt tgtggacact gctcggaggc tgacccgcac cgccccaatc
    13501 aaaaaccatg ccgtctgaaa cctttccgtt tcagacggca tggttttaca gcaatctaaa
    13561 cagggcggac cggagtcaaa aacacacctt cgccatttcc gcacaagcac ttcccctata
    13621 cgctcccaac cccaatccgc cgcattccag acggcattat agtggattaa attttagggg
    13681 ctgtactaga ttagcagata tgttaccctc gaaatatgaa gataacgcac tgcaaattaa
    13741 agaaaaaagt acaaaaagaa ctgctccgtt tttttgtgct ggaagttacc gtccgttctg
    13801 ccgccgatat tttgggtatt catcccaatt cggcagcact gttttaccgt aaaatccgca
//
LOCUS       DAWFJR010000046        13488 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWFJR010000046 DAWFJR010000000
VERSION     DAWFJR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13488
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.046"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(255..806)
                     /locus_tag="WGR04_001254"
     CDS             complement(255..806)
                     /locus_tag="WGR04_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0794805.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEAARVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKVSELACKTVETEARLEELH
                     NRKKALIDEMAREADKKELPKRL"
     gene            complement(820..2466)
                     /gene="pgi"
                     /locus_tag="WGR04_001255"
     CDS             complement(820..2466)
                     /gene="pgi"
                     /locus_tag="WGR04_001255"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0794806.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            complement(2587..3435)
                     /gene="hexR"
                     /locus_tag="WGR04_001256"
     CDS             complement(2587..3435)
                     /gene="hexR"
                     /locus_tag="WGR04_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ0794807.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            complement(3485..4471)
                     /locus_tag="WGR04_001257"
     CDS             complement(3485..4471)
                     /locus_tag="WGR04_001257"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ0794808.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGTSPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            complement(4452..5147)
                     /gene="pgl"
                     /locus_tag="WGR04_001258"
     CDS             complement(4452..5147)
                     /gene="pgl"
                     /locus_tag="WGR04_001258"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ0794809.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            complement(5427..6872)
                     /gene="zwf"
                     /locus_tag="WGR04_001259"
     CDS             complement(5427..6872)
                     /gene="zwf"
                     /locus_tag="WGR04_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ0794810.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTAGFLAKAETNSKIHIKQNFSDEAWASFVKRIEYLKVDVTEAADFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQACENLAAIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
     gene            7388..9223
                     /gene="edd"
                     /locus_tag="WGR04_001260"
     CDS             7388..9223
                     /gene="edd"
                     /locus_tag="WGR04_001260"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ0794811.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVSGELNVLIDEAE
                     WNAREIERIDLSANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
     gene            9404..10042
                     /locus_tag="WGR04_001261"
     CDS             9404..10042
                     /locus_tag="WGR04_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ0794812.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAS
                     HNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            10057..10275
                     /locus_tag="WGR04_001262"
     CDS             10057..10275
                     /locus_tag="WGR04_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794813.1"
                     /translation="MPSEALSSLRRHFAAWRRSRHTRQHHSDILPLKCPSAFRIIKIT
                     FSSFDSSRTAHTTRLKKCRLKRIQTASA"
     gene            complement(10299..11244)
                     /locus_tag="WGR04_001263"
                     /pseudo
     CDS             complement(10299..11244)
                     /locus_tag="WGR04_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            11407..12447
                     /gene="mutY"
                     /locus_tag="WGR04_001264"
     CDS             11407..12447
                     /gene="mutY"
                     /locus_tag="WGR04_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ0794814.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFAQDGNPQDKKF
                     ENALWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLLITPFEAQMPSESPSDGIWIKPTHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            12864..13424
                     /locus_tag="WGR04_001265"
     CDS             12864..13424
                     /locus_tag="WGR04_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ0794815.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     SHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
BASE COUNT         3136 a         3659 c         3415 g         3278 t
ORIGIN      
        1 cggtttaaat ttaattcact ataaaaacga caaaaacgca aaagccgccg acattcccgc
       61 atccaagttt cagtcaatca gataaccttg gatttctttg gttttcgcat tgatttctct
      121 ggtacggcag tcagattgtg ccacgccgta ttcgtcgccg tcggcgcatt tggcattcaa
      181 accgttttgt cagttgcggt actcgggcat gacggtttcg gcaatatcgg cgggcagtgc
      241 ctgaaccgcg ctgttcagag ccgctttggc agttctttct tgtccgcttc cctggccatt
      301 tcgtcgataa gggctttttt acggttgtgc agctcttcca agcgtgcttc ggtttccacc
      361 gttttgcatg ccagttcgct gacttttatg ccgtttggcg tttcaccttc ggctttcgca
      421 ttgttggcac agattttgtc cataccgctt ttccatgttt tctgcgaagc ttgcagttta
      481 ttctgtacag tttgcggcaa tcctttccag aactgctcga actcgctgcg cgacaacttg
      541 tagcgttctt cccattcgga tacgcgggcg gcttcctgct cccgacccaa tgcctcctgc
      601 gccgccgctt cttctgccgc ttcaagttct tcgttccttt gtttcgcttt gtccaacggc
      661 tctttaatca gtgccatagc tgcggaacat atatgtttaa atttatgcaa accatcatat
      721 cgggattgca cacgctctgc aagtttaccg acggttttcc tgttcgataa aaatgccgtc
      781 tgaaacggtc ggcgttcaga cggcattttt ccgcaggttt tatttgcggt tggtctgcag
      841 gtagaggttg atcaggcgtt cggtcgaact gtcgtgcttt tgcggcccgg tttcgccggt
      901 cagttcgccc aaaatggttt tagccagttg tttgccgagt tccacgcccc actggtcgaa
      961 gctgttgatg ccccaaatga tgccttgtac gaaggttttg tgttcgtaca tggcaatcag
     1021 gctgcccata ttgcgcgggt tgaccttgtc catgagaatg aggttggtcg ggcggttgcc
     1081 ggagaaggtt ttgtgcggaa ccagctcttc gatacgcgct tcgtccatac cctgcgcttt
     1141 gagttcggcg cggacttcgt cgggggtttt gccgcgcata aaggcttctg cttgggcgaa
     1201 gacgttggca agcaggattt cgtggtgtcc gggcaggttg ctgcgttttt caagcgaggc
     1261 aatcaggtcg atgggggtaa tgtgcgtgcc ttggtgcagc agttggaaaa aggcgtgctg
     1321 gccgttaatg cccgtttcgc cccagataat cggcgaggtt tcgtgtccga ctgctttgcc
     1381 gtccaacgta acctgtttgc cgttgctttc catatcgagc tgctggatga atttgggcag
     1441 gcggtgcaga tgttggtcgt aaggcgcgat gacgtggctg ccgccgccgt agtagttgat
     1501 ataccagatg ccgatgaggg cgagaatgac gggcaggttg cgctcgagcg gtgtgttgat
     1561 gaagtgttgg tccatcaggt gcgcgccgtt gagcatttcg atgaagtttt cttcgccgag
     1621 atacagcata atcggcaatc cgatggcgga ccacaggctg taccgaccgc cgacccaatc
     1681 ccaaaattca aacatattgg cggtgtcgat gccgaattcg gcgacggctt tttgattggt
     1741 ggaaacggcg gcgaagtgtt tggcaacggc ttcttcgtcg cccgcatgat tcaaaaacca
     1801 ttcgcgcgcg gtcagcgcgt tggtcagcgt ttcctgcgtg gtaaatgttt tggaggcgat
     1861 gatgaacaac gtggtttcgg ggtggacttt ggacaatacg tcgcgcagtt gcgagccgtc
     1921 cacgttggag acgaagtgca tattgaggcg cggatgaccg aaaggtttga gcgcggtaca
     1981 catcatcagc gggcccaaat ccgatccgcc gatgccgatg ttgacaacgt cggtaatgac
     2041 ttggttggta tagcccagcc agcttccgct gcggacttcg tgtgcaaatt cgcccatacg
     2101 ttgcaaaacg cggttgactt tgggcatcac atcttcaccg tcaaccacaa tcggcgaatt
     2161 ggtgcggttg cgaagggcga catgcaggac ggcgcggttt tcggtggtat tgattttttc
     2221 gccgtggaac atctgccgca tccgctccgg cacgcctgct tctcgggcaa gctcgaacaa
     2281 aagcgacatg gtttcgtcgt tgatgcggtt tttggagtag tccagcgtca gtccgccgac
     2341 ttgcagccag tagcgttccg cacgctgcgg gtcttgctcg aacatttcgc gcatatgcaa
     2401 tgttttgctg tcgtcaaagt ggttccacaa tttcgaccat gcgggtaagt cgtgaaggtg
     2461 tttcatctat atgctcctga atgaggtttt ttgttgtggg atgaaaaggc tgccggaaac
     2521 tgccgcaagc cgccgacggc cgttgttcgg catttcagac ggcatttgta ggatgccgtc
     2581 tgaaggtcaa tctttgtcgt aatcgatgtg cttgttgtgt atgctttttt tgcttttctg
     2641 caattgcagg ctggcagcat cgcccaagcg cagggcaagt ccgatggcga gaatgtcgat
     2701 gacggcaagc tgcaagaggc gggaaaccat gggcgtgtag agttcggcat tttcctgtgt
     2761 ggcaacgctc aacacgcagt cggcaagttg cgccagaggc gaatcgttgc gggtcagtgc
     2821 gatgacggac gcgccgtttt ctttggcgat gctgaccgca tccaaaagtt cgatagacga
     2881 acccgtgttg gaaatggcaa ccaaaacatc ctgatcgctc aaaacggatg ccgccatcag
     2941 ctgcgtgtgc gtatcgacat aggcgaccgt ggacattccg aaacggaaaa acttgtgttg
     3001 cgcgtcctgt gccacaatgc cggaattgcc gacaccgtaa aactcgacgc gacgggcgtg
     3061 catcagcgtg gcaatggcgt tttccagctc cgactctttc aggaagcggc gttcgcccaa
     3121 cagcgaggcg gcggcattgc ccaacacttt ctcgaccacg cttgccatat cgtcgtcggc
     3181 gttgagttct tcgtggacat agggcatacc ctcatgaccg atgctggcgg acaaggcgag
     3241 cttgaactcg ggcagccctt tataacccaa gctgcggcag aatcggatga cggtcggctg
     3301 gctgacggac gcacgttcgg caatttcggc aacggcggca tggacgaacc atttgggttc
     3361 cgccaatgca cattcggcga ctttgcgttc cgcaccggaa aggtttgcca gtgattcgct
     3421 gattttgctt aacataatga tatgcccttc gataatgcag ccccgctgca aggagccgct
     3481 gtggttaaac gtttctcaaa tggttgtcaa gagccgcagc cgcaccggaa attccgggaa
     3541 actcgctcaa gacgacatac acgggaatcg cggcaagata tgcttcaaac ctgcccttgt
     3601 tctcgaaacg gctgcggaac ggggaagttt tgaagtattc caacacgcgg ggaataatgc
     3661 cgccgcacag gtacacgccg ccgcgcgcgc ccagcgtcag ggcgaggttg gaagcaaccg
     3721 tgccgagcat ggcgcagaag atgtccaaag tctgacggca caaaggcgac gtgccgctca
     3781 aagccttttc cgtgatttca gacggcatca gtttggcggg tttggctttc tgttttgcag
     3841 ccaaagcctc gtaaaccaag ctcaagcccg cgccgctcaa aaagcgttcg gcggaaacat
     3901 ggccgtattt gtttttggcg tactgccaaa tcagcacttc catatcgtca aacggcggga
     3961 aactggtatg cccgccctcg cccgccaaag ccacccagcc tgcgtggctg tgcaccaatc
     4021 cgctcacgcc caggccggta ccggggccga taacggcttt gggggcaaat tcgacaggct
     4081 tttgcccgcc tacctgcatc aggtctttgc ttgaagtctg cgttaccgcc aatgcctgcg
     4141 cggtaaagtc gttcaaaagg atgagggtgt ccagccccaa agtctgacgg gtggtttcga
     4201 tggaaaacgc ccaatggtgg ttggtcatct gcacccagtc gcccaaaatc gggttggcga
     4261 tggcaaatgc cgcgtgccgt acggctgttg caccgctttg attcagatag gcacgcaccg
     4321 catcggtaac cgtatcgtag tctttacacg gaagcacggc ggctttttca atgacgcgcg
     4381 gcgcggtttc cagcgcaaag cgtgcattcg tcccgccgat atcggcgacc agtcggggat
     4441 atccggcttg tttattcggc gtagaagaca tggcagttca ctccttgatg gttcaaaacg
     4501 aggttgatcg gatattcgcg gttttcgcct tgtgcggctt ggtcgaacac ggcttttttc
     4561 tcttcgcccc gtatcgccaa aaacacatgc cccgtatggg caatcgcatc caaggtcata
     4621 ctgacgcgct cgtgcggcgc ggtaacgggc gtggtatgca ccagcgcgac acctgccgaa
     4681 ccgtcgattg ccgtctgaaa ctgcggagct ttcgggaaaa tcgaagccgt atgcccgtcg
     4741 tttcccatac ccaaaaccaa aacatcgggc tgtttgtaat gtttcagtgc ataatcgaca
     4801 acagcatcgg gatgtaattc ggtttcagtt tttccgtctt ccaccatagg aatccacatt
     4861 gccgcttccg ctttgttctt caacaggtat tcgcgcacca aaccggtatt gctgtcggcg
     4921 tggacggtcg gcacgatgcg ttcatctgcc aaggtgatgc cgacgttttt ccaatccaaa
     4981 tctttttgcg acagggcgtt gaaaaatgca atcggcgaac gtccgccgga aactgccaac
     5041 accgcaccgc ccttctcgtc cagtgcgccc tgcaaagcat ccgccactgc gtcagccaaa
     5101 gactgcgccg cttctgccgc attttcgtat tcgtgccaaa caaacatatt tgtgtccttt
     5161 ttttatttca gacggcatat tccgttatgg aaatgggttg agcaatatgc cggccgaaca
     5221 gttgtttatg cttttgatac caaatatcgg gactgctttt tatagtggat taaatttaaa
     5281 ccagtacagc gttgcctcgc cttgccgtac tatttgtact gtctgcggct tcgtcgcctt
     5341 gtcctgattt ttgttaatcc actatactgc tttacttacg ttcagacggc atttcaaacc
     5401 ccatgccgtc tgaacgcatt attgtattac tgctcttcgt gccacttgtg tccgtcgcgc
     5461 gccaatagtt cgcgcgcggc ttcaggcccc cacgagtgtg cgccgtagcc gtgcggcggc
     5521 gtggtgttat ttgtccagtt ttccaaaatc ggcatcacat attcccacgc ggcttcaagt
     5581 tcgtcgcggc ggttaaacaa agcgagtttg ccgttaatca catccagcag caggcgctcg
     5641 taagcttccg cgcggcggct ttccaatgct ttgcccaaat cggttgccag cggcacggtt
     5701 tcgaccttat ttcctgcccc cggggttttc atctgcgtat agaggcgcac ggattcatat
     5761 ggttgcaact cgataacgag ccggttgggc gcggtgcgac tgccttcaaa aatatggctg
     5821 ttcaaatctt tgaagttcaa aacgatttcc gccactttgc ccgccatgcg cttgccggta
     5881 cgcaggtaga agggaacgcc cttccagcgt tcgttttcga tttcggcttt aatggcgacg
     5941 taggtttcgg taaagctgtc ttgcggaacg ttgatttctt caagatagcc gttcatgcct
     6001 ttggcggcgg tatattgtcc gcgcacgaca ttttcattga cagattcgac ggtcagcggc
     6061 ttcaatgact tgatgacttt gactttttca tcgcgcaccg cgtcggcatc caagctggcg
     6121 ggggcttcca tcgcagtcat gcacagcatt tgcatcaagt ggttttgcac catatcgcgc
     6181 aacgcgccgg taatgtcgta aaactcgccg cgttcttcca cgcccaactg ctcggcgatg
     6241 gtcagctgca cgctttcgat gtatttattg ttccaaagcg gctcgaacat cacattggca
     6301 aaacgcaagg ccagcaggtt ttgcaggctt tctttaccta aataatggtc gatacggtag
     6361 atttgccctt ctttgaaata acgcgcaaca tcggtattga tttgttggga agatgccaaa
     6421 tccgtaccca acggtttttc caaaactacg cgcacattgt cggcattcaa accgatcgca
     6481 gcaaggtttt cacaggcttg cgcgaagaat ttgggcgcgg tggacagata gatgacgacg
     6541 ttgtcggttt ctttgcgcgc tttgaccaaa tcgcccaaag cggcaaaatc ggctgcttcg
     6601 gtaacatcaa ccttcaggta ttcgatgcgt ttgacgaacg aagcccacgc ttcatctgaa
     6661 aaattttgtt tgatatggat tttagaattc gtttccgctt ttgccaaaaa accggcggta
     6721 tccaattcgc tgcggctcac acccaaaata cgaccttcgg gatgaagcaa accggccacg
     6781 tgcgcctgat acaggcaggg caaaagtttg cgcattgcca aatcgccggt cgcaccgaac
     6841 aacaccaaat caaaatttgt ttgtgtactc atcgcattat ctcgtcagga aagaattttt
     6901 cgatgccgtc tgaaacctgt tttccccatc acgctgcatc gcaatatcgg aaacaaaggc
     6961 aggcggcata atgagtagta atactacaca ccgctacact ttttgtctat tcccattttt
     7021 acaatttatt tgacctagtc caaaaatcgg gcaggtttcc cctattccgt tacaacaatc
     7081 gaaagattct gcgatttaaa tcaaatttct tttcgatgtc tgattttttt gtaacaaaat
     7141 tacaaatttt gtactataat aacacccgct tcccactttc agacggtata ccttttaaaa
     7201 tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta cagataatac
     7261 ggcaaggcga ggcaacgccg tactggtttt tgttaatcca ctatactcac cgtctgaata
     7321 cccgatacaa aaatcagaaa cgcacaaaca aatccccaat accccccgtt ccgacaggag
     7381 accgaccgtg aacactactc ctatccactc caaactcgcc gaaatcaccg ggcgcattat
     7441 tgaacgcagc cgtccgacgc gtgaaaaata tctggcgaag atccgcagtg ccaaacaaat
     7501 gggacgctta gagcgcaacc agctcggttg ctccaacttg gcgcacggct atgctgccat
     7561 gcctaaaagt atcaaaatcg aaatgcttca ggaaaccgtc cccaacttag gcatcatcac
     7621 cgcctacaac gacatggttt ccgcacacca gccgtttaaa gacttccctg accaaatcaa
     7681 agacgaagcg cagaaaaacg gcgcgaccgc ccaagtcgcc ggcggcacac ccgccatgtg
     7741 cgacggcatc acgcaaggct acgccggtat ggaactgtcg ctgttctccc gcgacgtgat
     7801 tgccatgagt accgccgtcg ggctgtcgca tcaaatgttt gacggcagcc tgtttatggg
     7861 cgtatgcgac aaaatcgttc caggtttgat gataggcgcg ctttcgttcg gtcatattcc
     7921 gggtatcttc gtccccgcag gcccgatgtc cagcggtatc ggcaacaaag aaaaagcccg
     7981 cacccgccag cttttcgccg aaggcaaggt cggacgcaac gaacttttga aaagcgaaat
     8041 gggttcttac cacagcccgg gcacctgcac tttctacggc acggcaaact ccaaccaaat
     8101 gatgatggaa atgatgggcg tgcacctgcc tgccgccgcc ttcgtccacc cttacaccga
     8161 cctgcgcgaa gcgctgaccc gctacgccgc cggacacctc gcgcgcggca tcaaaaacgg
     8221 cacgattaaa cctttgggcg aaatgttgac cgaaaaatcc tttatcaacg ccttgattgg
     8281 cctgatggca accggcggtt cgaccaacca caccatgcac ctcgtcgcta tggcgcgtgc
     8341 ggccggcgtg attttgaact gggacgactt cgacgaaatt tcctccatca tcccgctgct
     8401 catccgcgtt tatccgaacg gcaaggccga cgtgaatcac tttaccgcag cgggcgggct
     8461 gcctttcgtt atccgcgaat tgctgaatgc aggcctgttg cacgacgatg tcgataccgt
     8521 cgtcggacac ggtatgcgcc actacaccaa agagcctttc ctcatcgacg gcaaactcga
     8581 atggcgcgaa gcccccgaaa ccagcggcaa cgacgacatc ctgcgcaaag ctgacaaccc
     8641 gttctccccc gacggcggtc tgcgcctgat gaaaggcaac atcggacgcg gcgtgattaa
     8701 agtgtccgcc gtgcgcgaag gttgccgcat cattgaagcg cctgccatcg tgttcaacga
     8761 ccaacgcgaa gtgttggctg cgtttgaacg cggcgagttg gaacgcgatt ttgtgtgcgt
     8821 cgtccgctac caaggcccgc gtgccaacgg tatgcccgaa ttgcacaaac tgaccccgcc
     8881 tttgggcatc ctgcaagacc gcggcttcaa agtggcgctg ctgaccgacg gtcgtatgtc
     8941 cggcgcatcc ggcaaagttc cggcctccat ccacatgacg cccgaagccc tgatgggcgg
     9001 caacatcgcc aaaatccgta ccggcgacct gatccgcttc gactccgtta gcggcgaact
     9061 caacgtcctg attgacgaag ccgaatggaa tgcccgcgaa atcgaacgta tcgacttgag
     9121 cgcgaaccaa caaggttgcg gccgcgaact cttcgccaac ttccgcagca tgaccagtag
     9181 cgcagaaacc ggtgccatga gcttcggcgg cgaatttgcc tgatgcgcgt ttcagacagc
     9241 cttttcagac cgaaggtcgt ctgaaaaatt attcaagcgt tttaagatag acgcaggtcg
     9301 gattctcgaa tccgacacag ccgtccaaga tgtcggtttc ttgaatccga cctacaacct
     9361 gtcccatctt aataaaacat cccaccccac ccggagaacc gaaatgtcca aactgacccc
     9421 ccgcgaaatt ttgaccgccg gcgcagttgt gccggtaatg gcgattgacg acttaagcac
     9481 cgccgtcgat ttgtcccacg cccttgtcga aggcggcatc cctaccctcg aaattaccct
     9541 gcgtacaccc gtcggactcg atgccatccg cctgattgcc aaagaagtac ccaacgccat
     9601 cgtcggcgcg ggtacggtaa ccaatcccga acagcttaaa gccgtcgaag acgcaggcgc
     9661 ggttttcgcc atcagcccgg ggctgcacga atccctcgcc aaagccagcc acaacagcgg
     9721 catccccctg attcccggcg ttgccacccc gggcgaaatc caactggctt tggaacacgg
     9781 catcgacacc ctcaaacttt tccccgccga agtcgtcggc ggcaaagcca tgctcaaagc
     9841 cctgtacggc ccttacgccg atgttcgctt ctgcccgaca ggcggcatca gcctcgccac
     9901 cgcgcccgat tacttggcac tgcccaacgt cctgtgcgtc ggcggctcat ggctgacgcc
     9961 gaaagaagcc gtgaaaaaca aagactggga caccatcacc cgtctcgcca aagaagcggc
    10021 ggcattgaaa cccaaagcct gaaccgcatc gtaaaaatgc cgtctgaagc cctttccagt
    10081 ctcagacggc attttgccgc ttggcggcgc agtcggcata cacggcagca ccactccgac
    10141 atactgcccc taaaatgccc atccgccttc cgcataataa aaataacgtt cagttcattc
    10201 gacagcagcc ggacagccca tactacgcgg ctgaaaaaat gccgtctgaa acgcattcag
    10261 acggcatccg cttaaaaaaa caactgattc aaggccgatt aatccgcttc caaaaccact
    10321 ttcatcgctt gattttcggc ggcgtgtttg aacacgtcgt aggctttttc caattcgctg
    10381 aatttgaaac tgtgggtcaa cattttggtg taatcgacgg agctgctgga aattgccttc
    10441 atcagcattt cggtggtatt ggcgtttacc aggccggtag tgatggcaag ctttttaatc
    10501 cacagttttt ccagtttgaa atcaacggat tgaccgtgta cgccgacaac ggcgatatgg
    10561 ccgccgggtt tcacaatgtc ttggcacata ttccaagttg cagggatacc gacggcttcg
    10621 atcgcgcagt ccacgccgtc ttcgccgacg atagcggcaa cttgcttgct tacatcgccg
    10681 gaagcagggt taatggtatg ggttgcgccc aactctttcg ccagtttcaa acggttttcg
    10741 tccatatcgc aaacaatgat ggcggcgggg ctgtacagtt gggcggtcgg caaagcgtcg
    10801 ctcaacaaca gggcgacttc ttcgttgacg ttgtcgggca gcggcacgag gctgttgtcg
    10861 gcataaggcg tgcggacgta ttctgcctgc gtgccgtcaa tcatgtagcc caaaatccag
    10921 ccgccgttgc ggcaatgcga ataaagttgg attttgcagt tgtcgcaagt acagcatttg
    10981 ctgacgcatg aaataatgac tttatcgccg actttgatgt tttttacagc atcgccgact
    11041 tcttctacaa taccgatgcc ttcatgaccg agaatacggc cgtcggcgac ttcgggtttt
    11101 tttgcctttc caaataccca aatcggtacc gcaaatcgtg gttttgacga ttttcaccac
    11161 cgcatcggtc ggatcgataa tctgcggacg gggtttttct tcaaaacgga tgtcgtttgc
    11221 gccgtgataa accattgctt tcatgctgat actccttgct tgttgataaa taatttcaat
    11281 accgcaataa agtttcttta tatgagttat atgcccctac aaaaaataag tcaataagaa
    11341 ttattttcac aatgttatac aataacatac tgttttaaat ataaataaaa ccaccgattg
    11401 atattaatga acacacccat ccccttctcc gaacggctca tccgctggca aaaacaacac
    11461 ggtcgccacc acctcccttg gcaggtcaaa aacccttatt gcgtctggct ttccgaaatc
    11521 atgctccagc aaacgcaggt cgccaccgtg ttggactact atccgcgttt cttagaaaaa
    11581 ttcccgaccg ttcagacgct tgccgccgcg ccgcaagacg aagtgttgtc gctgtgggcg
    11641 ggcttgggct attacagccg cgcgcgcaac ctgcacaaag ccgcgcaaca agtcgtcagg
    11701 caattcggcg gcacgtttcc gtcggagcgc aaagacttgg aaacgctctg cggcgtaggc
    11761 agaagcaccg ccgccgccat ttgcgccttc tccttcaacc gccgcgaaac cattttggac
    11821 ggcaacgtca aacgcgtact ctgccgtgtc tttgcccaag acggcaatcc gcaagacaaa
    11881 aaatttgaaa acgcgctctg gacacttgcc gaaagcctgc tgccgtctga aaacgccgat
    11941 atgcctgcct atacacaagg tttgatggat ttgggcgcga ccgtgtgcaa acggacgaaa
    12001 cccttgtgcc accaatgccc gatggcggac atctgcgaag cgaaaaagca aaaccgcacc
    12061 gccgagctgc cgcgcaaaaa aaccgccgcc gaagtaccga ccctgccgct ttactggctg
    12121 attgtccgca accgggacgg cgcgattttg ctggaaaaac gccccgccaa aggcatttgg
    12181 ggcgggctgt attgcgtgcc gtgttttgaa agtttgaacg ggctttccga ctttgccgcc
    12241 aaattctccc tgaccatggc agatatggac gaacaaaccg ccctgaccca ccgcctgacg
    12301 caccggctgc tattgattac gccctttgaa gcacaaatgc cgtctgaaag cccttcagac
    12361 ggcatttgga taaagccgac gcatttgaaa gattacggcc tgcccaagcc tttggaaatt
    12421 tatttaaacg gtaataggtt agaataaaca aaataaaccc attgaactgt tgtttgcagg
    12481 tatcgcagca agaacaaccg atgaatttgg gtcgtatttt aggcggcggg ataatgttca
    12541 aatgggacat ttggaacgga agaagtcggc aatttaaaaa ggatttaaaa agcaaagaag
    12601 gtcaaaaaca tgaacacaaa cttaaatgac aaagacaaag ccatggatac cgcaatcagg
    12661 tttcagaaaa ggatgaggat tccgaaattt ttctttttaa ttctcggaat cacaatggtt
    12721 ttggcattta tccaagacgt gataacgggt tctaattttc tgcaaataac aattaatgta
    12781 aaattttcgt aaaaatttat cggcttttaa aacaaaattg actaaaatag tcgcgagttt
    12841 ttactgcaat aaaggagatt gcaatgaata tgaaaacctt attagcacta gcggttagtg
    12901 cagtatgttc agttggtgtt gcgcaagcac acgagcataa tacgatacct aaaggtgctt
    12961 ctattgaagt gaaagtgcaa caacttgatc cagtaaacgg taacaaagat gtgggtacag
    13021 tgactattac tgaatctaac tatggtcttg tgtttacccc tgatttacaa ggattaagcg
    13081 aaggcttaca tggtttccac atccatgaaa acccaagctg tgagccaaaa gaaaaagaag
    13141 gtaaattgac agctggttta ggcgcaggca gtcactggga tcctaaaggt gcaaaacaac
    13201 atggttaccc atggcaagat gatgcacact taggtgattt acctgcatta actgtattgc
    13261 atgatggcac agcaacaaat cctgttttag caccacgtct taaacattta gatgatgttc
    13321 gcggtcactc tattatgatc cacacgggtg gtgataatca ctccgatcat ccagctccac
    13381 ttggcggtgg cggcccacgt atggcatgtg gcgtgattaa ataattcgat tgttcgaaac
    13441 gaaaagtgcg gtaaattttg accgcacttt tttgctagat atttagca
//
LOCUS       DAWFJR010000047        13452 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWFJR010000047 DAWFJR010000000
VERSION     DAWFJR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13452
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.047"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(173..601)
                     /locus_tag="WGR04_001266"
     CDS             complement(173..601)
                     /locus_tag="WGR04_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ0794816.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(666..2414)
                     /locus_tag="WGR04_001267"
     CDS             complement(666..2414)
                     /locus_tag="WGR04_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ0794817.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHADSTALLADRHSLPPFGMTDADDAYTTLLGLFSHEYFHAWNV
                     KSIKPAAFAPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     IQQTRGCLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTIMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFAAQWARYPVGAKINIHFFRAGILRQTVLTVQATAADTA
                     ILHITDRNLLDNWLFG"
     gene            2741..4174
                     /gene="ccoN"
                     /locus_tag="WGR04_001268"
     CDS             2741..4174
                     /gene="ccoN"
                     /locus_tag="WGR04_001268"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ0794818.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4201..4812
                     /gene="ccoO"
                     /locus_tag="WGR04_001269"
     CDS             4201..4812
                     /gene="ccoO"
                     /locus_tag="WGR04_001269"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ0794819.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4817..4987
                     /locus_tag="WGR04_001270"
     CDS             4817..4987
                     /locus_tag="WGR04_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ0794820.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            5011..6108
                     /gene="ccoP"
                     /locus_tag="WGR04_001271"
     CDS             5011..6108
                     /gene="ccoP"
                     /locus_tag="WGR04_001271"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ0794821.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6148..6342
                     /locus_tag="WGR04_001272"
     CDS             6148..6342
                     /locus_tag="WGR04_001272"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ0794822.1"
                     /translation="MPGIPHVGIKADWADRIKKGKDTLHKHAIEGFNTMPAKGGRGDL
                     SDDEVKAAVDYMVNQSGGKF"
     gene            6544..7953
                     /locus_tag="WGR04_001273"
     CDS             6544..7953
                     /locus_tag="WGR04_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0794823.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGQEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRAEDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYHTMDEIHSDMMLDSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIQAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPSGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            8054..11263
                     /gene="recC"
                     /locus_tag="WGR04_001274"
     CDS             8054..11263
                     /gene="recC"
                     /locus_tag="WGR04_001274"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ0794824.1"
                     /translation="MFYLYQSNRLETLAALFAHIQKVKPLKSALQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLAFSLPASLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMHWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPFFYTLECLSDLLESRFEVDKVLALLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAAFLETLTDIVRIWRQPATVDEWVGRCRGLLKTLFQTEADDQKAVQNLE
                     NEWVKWQAETELAQFSGQLPPHTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNLIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAEIYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPLPYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILVEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLATAEAFAQKQDWEAALKKAQTAYHGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTLAAAEKKEEAGEA"
     gene            complement(11333..12901)
                     /gene="mtrF"
                     /locus_tag="WGR04_001275"
     CDS             complement(11333..12901)
                     /gene="mtrF"
                     /locus_tag="WGR04_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ0794825.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13235..13447
                     /locus_tag="WGR04_001276"
     CDS             13235..13447
                     /locus_tag="WGR04_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794826.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYENIARDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
BASE COUNT         3372 a         3247 c         3566 g         3267 t
ORIGIN      
        1 tgcattttga aaatgagttt gagcataaaa ttttagtaac ctatgttatt gcaaaggtct
       61 cccactgtaa aaacggagaa ggacagagga cggcagtaag gtttgccgtc ctctttttca
      121 agttgcggtg gaatgccgct tgaaggtgtg gtttaaacgt ttcggcttgt aatcagacgc
      181 gggcgttgtc cagccgctct ctgaagtttt ccaaatttac gcctgccgct tgggcgcggc
      241 ggatgatttc gtcaaccggc ataccttctg ccgcccggcg gaagccccac aggagggagc
      301 agcgcgtacc ggtccggcaa taggcaagga cgggatattc ggcttgtccg atgagttggc
      361 ggaaggtttc gacatcgtgt ttttggatgt cgcgtgcggt aacgggttgg tgatggaatc
      421 cagtaacgcc tgcttgttcc agccactgtt tgatttgggc gaagtcgggt tgcgattctt
      481 cttcgcggtc ggggcggttg cagatgacgg ttttgatgcc cagttgcgcg atttgttccg
      541 catcggcttt ggtcagttgc ggagaaatat agaggtgttc gtcaagtttc agaatggcca
      601 ttttgtttcc tttactttgc ggttgttttt cagacggcat tttgtgtgca atgccgtctg
      661 aaagtttaac cgaacaacca gttgtccaac aggttccggt ctgtgatatg taggatggca
      721 gtatccgctg ccgttgcctg aaccgtcaag acggtttgac gcaatatgcc ggcacggaag
      781 aagtggatat tgatttttgc accgacaggg tatcgggccc attgtgcggc aaagtcggtg
      841 caggcataac cgtctaaagc aatgattttg tcttgcgggc atagtgccgc agattccgcg
      901 ctgccgccgt tgaagacatg ggtcaggacg atgtggtcgg tgttttgttt gaaacgtgcg
      961 ccaaaatcgc ctgccgacgg gacggggcag atgtgttctg cgtatccgcc gccgtgttgt
     1021 cggggaagcg gcaggaaggt cagtcccacg cctgcggttg ccaggcattc ggcaagcggc
     1081 aaatcttcgg tactgtataa cgctttttgg aaaaaatctt ccaaatccaa gccggtaatt
     1141 tcctgacagc ggatttgcca gtgtttttcc ggaatacccg agtgtgtgtc cctccactcc
     1201 cgatagagtt tgtccattat cgtatcgaga gaatgtctgc cgttgcttcg gttgcgtatt
     1261 atcagatcaa ggcacaatgc ggcaagcgcg cctttctggt agtagctgac gatggcgttg
     1321 gggctgtttt catcaggttt gtaaaatttg ttccacgcgg taaaactcga ttccgccaag
     1381 gtctgcctca aacagccgcg ggtttgttgt atgcgcgtaa tgccttgtgc cagcaggttt
     1441 aaataagatt cgggcgagat ggtgcggctg cgtgccaaaa acaaatcgtc gtaataggat
     1501 gtaatgcctt cgaatgccca tagttgttcg gtatagtttt ctttgtcgag gtcataaggg
     1561 gcgaacgcgg caggtttgat ggatttgacg ttccacgcgt gaaaatattc atgggagaaa
     1621 agtccgagca atgtggtgta ggcatcgtcg gcatcggtca taccgaacgg cggaaggctg
     1681 tggcggtcgg cgagcagggc ggtgctgtcg gcgtgttcca aaccgccgta aatatggtcg
     1741 ccgacgtgga gcaggaacaa atatttttga aacggggcag gggaggaaaa caccgccagt
     1801 tctgtttcgc agattttttt gatatccgaa accagcctgt tgcggtcgaa atcgggatag
     1861 atgccgctta aggcaattgt gtgcggaatg cctgccgcct caaaatctaa aaattcaatc
     1921 aagcccatct cgacaggtcg gtcaatcaat tcggcataag atgccgcctg aaagacaaac
     1981 ctccctgttt ccggcagcgt tgtggcaata cgccattcgg acggaatacc cgtcaattcc
     2041 aagcggtgcg gcagcgtttc cgttccttcg actttcaaaa acaggcacga tccgtcaaaa
     2101 aaaccgcgtt ctgtcgtcag gaaagaacct cgaaccgaca aatcgaatgc atatacggtg
     2161 tagcggattt gccactcgcc gcgtacggcg gcagcgtgcc agcggttttt tgcaatttgt
     2221 tcgagcggca tggacgtgcc gttacaggat gcatggatag aagtgatgtg gcgggaaaaa
     2281 tcccgaatca gatagctgcc cggaacccag tttggcaggc ttatttctat aggaagatca
     2341 tcatcttgtg tgaatgtcag caggatgtgc cactcgtggt cgagagggga cggggttatt
     2401 ttataatgaa tcataactat caaatatatc gttaaacggg atggattaaa tacctttaaa
     2461 atgcagctta acctacatca ataaagtgcc acaaaaaggg aaaattggaa acaaaagcaa
     2521 tggcggtcgg gttgtaattt attataacaa tatgatttat atgtaataaa aaacagatta
     2581 agcaaagggc tcgccggcgg cgcgaatgta attccgcttg gcgtaataag gcagatatgt
     2641 taaatttaga aaaaattacg cttgcccgga ttgcacctta aacaggctgc ggaagcggca
     2701 ggataaaaat ctttattccc ataaattcat ggagactctc atggacacac aaacttacaa
     2761 ctacaaagtg gtgcgccagt tcgccatcat gactgtagtt tgggggattg tgggtatgtt
     2821 ggtcggcgtt atcgtcgccg cccagctttt tgctcctgcc ctcgacttgt ctaatatcgg
     2881 accttggttc cacttcggcc gcctgcgtcc gctgcacacc aatgcggtta ttttcgcatt
     2941 tggcggttgc ggcctgatcg gcacatcata ctacgttgtc caacgtactt gtaatacccg
     3001 tcttttcggc ggttggctgc cggcatttac cttttggggc tggcaggcgg taatcgttgc
     3061 cgccgtcgtc agcttcccta tgggttggac ccaaggtaag gaatatgccg aactggaatg
     3121 gccgatcgat attctgatta ctttggtttg ggtggcttac gccatcgtat tcttcggtac
     3181 gattgccaaa cgtaagatta aacatattta cgttgccaac tggttctacg gcggctttat
     3241 tttggcggtc gcacttttac acatcgtcaa caatatcagc atcccggcag ggttgatgaa
     3301 gtcatacccc gtctattccg gtgcgattga tgctatggtt caatggtggt acgggcataa
     3361 cgccgtgggc ttcttcctga ctgccggctt cttgggtatg atgtactatt tcgtacccaa
     3421 acaagcagcc cgccccgttt actcctaccg cctgtccgtc gttcacttct gggcgttgat
     3481 tttcacctat atgtgggcgg gtccgcacca tcttcactac actgcgctgc ctgactggac
     3541 gcaatctttg ggtatggttc tgtctttgat tctgttcgca ccctcttggg gcggtatgat
     3601 taacggcatc atgaccttgt ccggcgcgtg ggacaaactg cgtacagacc cgattcttaa
     3661 attcctgatt gtatccttgt ccttctacgg tatgtctacc tttgaaggcc cgatgatgtc
     3721 gattaaaacg gtcaatgcat tgagccacta tacggactgg accgtcgcgc acgttcatgc
     3781 gggtgcgttg ggctgggtag gctttgtaac catcggttcc gtctattaca tgattccccg
     3841 tctgttcggc aaagaacaga tgcacagcac caagctggta gaagcacatt tttggattgc
     3901 gaccatcggc gtggttctgt atatcgctgc catgtggatt gccggtgtga tgcagggttt
     3961 gatgtggagt tctttgaacg atgacggtac gctgacctac tcgtttgtcg aatccgtaaa
     4021 acgcaccatg ccttactacg tgatccgttt tgcaggtggt ttattgtatc tgagtggtat
     4081 gtgcattatg gcgtacaacg tgtaccgcac agccatcggt ggtaaagcag tcgatgccga
     4141 aatccctgcg gtttcccaaa cacagcacca ctaaaatact aagaaagata ggctaccaaa
     4201 atgaaattac aacaattggc tgaagaaaaa atcggcgttc tgattgtgtt cacgctgctt
     4261 gtagtcagtg tcggtctgtt gattgaagtt gtgcccttgg cctttaccaa ggcggcaaca
     4321 cagccggcgc cgggcgtgaa gccttacaat gccctgcagg ttgccggacg cgatatttac
     4381 atccgtgagg gctgttacaa ctgccactcg caaatgattc gtccgttccg tgcggaaacc
     4441 gagcgttacg gtcattactc tgttgccgga gagtcggttt acgaccatcc gttccaatgg
     4501 ggttccaaac gtaccggtcc tgatttggca cgtgtgggcg gccgctattc cgacgaatgg
     4561 caccgtatcc acctgctgaa tccccgtgat gtcgtgcctg agtccaatat gccggcattc
     4621 ccgtggcttg cacgcaataa agtcgatgtc gatgcaaccg ttgccaacat gaaggctttg
     4681 cgtaaagtag gtactcctta cagtgatgag gaaattgcga aagcgcctga ggctttggca
     4741 aacaaatccg agctggatgc tgtagtcgcc tatctgcaag gattgggtct ggctttgaaa
     4801 aacgtaaggt aacatcatgg atattaacgg tattcgtgct ctcttcacgg tatggatctt
     4861 tatctgtttc ctgttggtac tctatatcgt cttcaacagg cggaataaga aaaactatga
     4921 taatgccgca aacagcattt ttgatgaaaa ccaagatgcg caagataaga aaagcgaaaa
     4981 ccgttaatat tgtgataacg gagcaaaaca atgaacacaa catcccaatt taccagtaat
     5041 ttctggaata tatatattgc agttattgtc ttactgagct ttatcgcttt ggcttggctg
     5101 ctgctgtctc aaaatgttgt caaacgtccg aagaagggcg aagaagtaca aactacgggt
     5161 catgagtggg acggcattgc cgaatacgac aatccgctgc cccgctggtg gttttggctg
     5221 tgtgttttga cgtggctgtt cggtatcggt tatttggtta tgtatccggg tgtcggcgac
     5281 tacaaaggtc tgctgaaatg gaccagccat aaccaatatg aaaaagaggt caaaaaagcc
     5341 gatgagcaat acggcaaact gtatgccaag tttgcggata tgccgattga aaaagtggca
     5401 aaagaccctc aagccaagca aatcgcccaa aacctgttta acacttattg tatccagtgc
     5461 cacggctctg atgctaaagg ctctaaaggt tttccgaatc tgaccgatag cgattggttg
     5521 tggggcggtg atcccgataa aatccacgag accatcgaaa aaggccgtgt tgcgactatg
     5581 cctgcctggg gccctgcttt gggcgaagaa ggcgtgaaag acgttgccca ttatgtgatg
     5641 tccctttcaa aacccgaagg tcagtatgat gaggaacgtg ccgcgcgcgg acaagccttg
     5701 ttcagcggtc cgcctgccaa ctgtttcact tgccacggcg ataagggaca aggtatccaa
     5761 ggtttgggtc cgaacctgac tgatgacgtg tggttgtggg gcggtacgca aaaatccatt
     5821 atcgaaacca ttaccaacgg tcgcagcagc caaatgcccg cttggggaca tttcttggat
     5881 aaagacaaac tgcatattat gactgcttat gtatggggtc tttccgataa agacggtaaa
     5941 gctccggtga aaaaagccga gcctgcaccg actcccgcac cggcggcaga acccgcagcc
     6001 tctgctcctg cagaagcagc acaagccgcg tccgaagcca aacctgccgc agcagaacct
     6061 aaagccgagg aaaaagctgc acctgctgcc aaagcggacg gcaaataggt ttatgaaacc
     6121 gtttgtgccg cctgccatgg caatgcgatt ccgggtattc cccatgtagg catcaaagcc
     6181 gattgggccg accgcatcaa aaaaggcaag gacacgttgc acaaacacgc gatcgaaggt
     6241 ttcaatacga tgcccgcaaa aggcggtcgc ggcgatttga gcgatgatga ggttaaagct
     6301 gcggttgact atatggtcaa ccagtcaggc ggcaaattct aacttgactg agtttccgat
     6361 attatcggtt atgaaaatcc agattcagta ttgaatctgg attttttgtt ttaacgggca
     6421 tccggtttta aacgcagact gcctatttgt tgtaatgaat tttgacttta gtcataaaga
     6481 attagtcttt attgtttata atcgcgcatc atcacgatag ccctattttc aaaaggacga
     6541 tttatggata cacaaatcaa gactgaagcc gacaatcaga gcaaccggcg ttatctgacc
     6601 gtttggcggt ggcattttta tgccggtctg ctggttatgc cttttcttac cctgcttgcc
     6661 gttacgggtt tgggtatgct gctgtttgcc aatattaccg gtcaggaggg cgagcggatt
     6721 catgttgtgc cgcaggcaac ggtacaacct ctgtctgttc aggcggaagc ggcacgcagt
     6781 gccgttaatc cggaaacttc gtccgtcgtt cagtatattg cgccgcgcgc cgaggacatg
     6841 gttgccgtgt tccgtgtcaa caatgagggt aaggcaacga tggtcgcggt cgatccttat
     6901 acggcaaaag tagtcagcac tatgccgcgc aatcagggtt ggtatcacac gatggatgaa
     6961 atccacagcg atatgatgct cgattcgaca ggcgattatc ttttggaaac ggctgcatcg
     7021 ctgacgatta tcatgataat cagcggtttg tatctttggt gggtgaaacg gcgcggcatc
     7081 caggcgatgc tgctgccgcc aaaaggcagg gcgcgttctt ggtggcggaa tctgcacggc
     7141 gcgtttggaa cttgggtgtc gttgatttta ctgttgttct gcctgtcggg tattgcttgg
     7201 gcaggtattt ggggcggcaa gttcgtgcag gcttggagtc agttcccggc aggcaaatgg
     7261 ggtgtcgaac cgaaccctgt ttcagtcgtg ccgacccacg gcgaggtatt gaatgacggc
     7321 aaggttaagg aagtgccgtg ggttttggag cttacgccta tgcctgtttc agggacgact
     7381 gtgggcaaag acggtattaa ccctgacgag ccgatgacat tggaaaccgt cgaccgtttt
     7441 gcgcgggaaa tcggtttcaa agggcgttat cagctgaatt tgcccaaagg cgaggacggc
     7501 gtatggactt tgtcgcagga ttctatgagt tacgacatga tcagcccgtt tgctgaccgc
     7561 acggtgcata tcgaccagta cagcggcaag attcttgccg acatccgttt tgatgattac
     7621 aacccgttcg gcaaatttat ggcggcgagc attgcgctgc atatggggac tttgggctgg
     7681 tggagcgtgt tggcgaacgt tttgttctgc cttgccgtca tttttatcgg tatcagcggt
     7741 tgcgtgatgt ggtggaaacg ccgtccgtcc ggcgcggtgg gcatcgttcc gccggcgcag
     7801 aaagtcaagc tgccggtttg gtggatgatg gcattgccgc tattggcaat cgcactgctc
     7861 ttcccgaccg cactgcttgc cattgccgtg atttggctgt tggatacctt gctgctgtcg
     7921 cggattcctg ttttgaggag atggtttaaa tgacccgatg ccgtctgaaa ggttcagacg
     7981 gcattttgtt tgaaggcagg cgggggaaag gctatataat cccgaatact tttccgcaac
     8041 ttttgtcgaa atcatgtttt atctgtatca atccaaccgt cttgaaacgc tggcggcact
     8101 gtttgcccac attcaaaaag tcaaaccgct gaaatcggct ttacagcccg aacagattat
     8161 tgtgcagagt caggggatgc gccgctacct caacacctgc ctcgcccgcg atttgggcgt
     8221 ggcggcgaat ttggcgttca gcctgcccgc cagcctgacg tggaagctga tgaaaaaact
     8281 gattcccggc attccggaac tcagcccgtt tgcacccgaa gtcatgcgct ggcggctgct
     8341 ggatttgttc cgcagcgaag cattccggaa tacggcagaa tttgaagatg tgaggaatgt
     8401 gctgcaagac tatctaggca gcggcgaatc ggcagattac cagcttgcgg gacagcttgc
     8461 ggacatattc gaccaatacc ttgtctatcg tccgcagtgg atagacgcgt ggcagcaggg
     8521 caggatactc ggtttgggcg acgatgagat ctggcagtcc aaactgtggc gttacctcga
     8581 cgacggcagg cagagcgcgc cgcaccgtgt cgcgttgtgg gaaaaactgc ttgccgcgtt
     8641 ggacaaggat aagctgcccg agcgttattt cgtgttcggc atttccacga tggcgccgat
     8701 gtatcttcag cttttgcaca agctgtccga acattgcgat gtgttcgtgt tcgcactcaa
     8761 tccgagcggg atgcattggg gcaatgtcat cgaggcggcg caaatcctca aaggtggcgg
     8821 cgatcccgat ttaactcagg cagggcatcc gctgctcgct tcattgggca agcaggggcg
     8881 ggactttttc gactttttga acgaaatgga aatagaaggc gaaacgccgg tatttgaaga
     8941 aggcggacgc gatacgcttt tgcacgccct gcaaaccgat attcaaaacc tgaaaatgcc
     9001 gtctgaaaat gtgggaagcg tcaacacggg cgacggctcg atacgcatcg tctccgcaca
     9061 cagccccttg cgcgaattgc agatactcaa agacaagctg ttgaaaattc tgcatgaaca
     9121 tcccgattgg cagccgcatg atatcgccgt attaaccccg aacatcgaat cctatacgcc
     9181 ttttatcgaa gccgtgttcg gacaggcgca gcccggtgcg caggcattgc cgtattccgt
     9241 ttcagacgtg aaaatcagcc gccgacaacc gtttttttat acattggaat gcctgtccga
     9301 cttgttggaa agccgatttg aagtcgataa agtgctcgcg cttttggaaa ccgcgcccgt
     9361 attgcgccgt ttcggactga ctgaggacga tttgccgctt ttgcacgata tggttgccga
     9421 tttgaacgtt cactggggtt tggacggaga aatgcgcggc ggcacggatc agctgttcac
     9481 ctggaagcag gcggtagaac gcatgatatt gggctggatg ctgcccgaag gcggcaaccc
     9541 gatgtggcag gatgtcagcg cgtggtatgc cgacgtgaac caaaccgccc tgttcggacg
     9601 ttttgccgcc ttcctcgaaa ccctgacgga tattgtacgg atatggcggc agcccgcaac
     9661 ggttgatgaa tgggtaggac gttgccgagg tttgcttaaa acattgttcc aaaccgaagc
     9721 cgatgaccaa aaggcagtcc aaaacctcga aaacgaatgg gtcaaatggc aggcggaaac
     9781 cgaattggcg caattttccg gacagttgcc gccgcacacc gtcatccgcc acatccgccg
     9841 ctttctcgac agcgaaagcg aggcaggctt tttacgcggc ggcatcacct tttgcagtat
     9901 ggtgccgatg cggagcctgc cctttaaagt catctgtctg ttgggtttga acgacggaga
     9961 ttttccccgc aataccaaag ccgccgtatt cgacctgatt gccaaacatc ccgccaaagg
    10021 cgaccgcgcc cgccgcgacg acgaccgcta cctcttcctc gaagccctca tcagcgcgcg
    10081 tgaaatcctg tacctgtcct acatcgggcg cgacatccgc aaagacgaag aactggctcc
    10141 gtcttctttg ttgggcgaac tcatcgatac cgttgccgct atggcgggca tcggtagccg
    10201 ccaacttgca caaaacctga tagaacagca tccgctgcaa gccttctcgc gccgatattt
    10261 tcaagaaggc ggacgttcag acggcatatt cggcacgcgt accgactacg ccgccgcgct
    10321 cggacaaacg cccgaaccgc cgcaaccctt tttcgatcaa cccgtagaaa acgccgaacc
    10381 tgttgccgaa atcggacagg acgaatttat ccgcttctgg cgcaaccctg tcaaagtatg
    10441 gcttcagcag cagcttgcgt ggagcgaacc ccatatcggc gaagcctggg agcctgccga
    10501 acccttcgag ccgcaacacg ccgatcaaat cgccgaaatc tacatcgaag cacggcgcga
    10561 aggacgggat tttgcccaaa ccgccgcccg catcggggcg gaaagcctcc tgccgtcggg
    10621 agagttgggc agactttggc agcaggactt ccaaaccgct gccaaacaaa tcgacacggc
    10681 ggttttaaac agtcccaaac ttcctccgct gccctacgcc ataccgtcgg acgggcaaat
    10741 cctgaaaggc agcttgggca atctgtaccg ctgcggacaa gtgttttacg cctacggcaa
    10801 acccaacgcg ccgcaacgta tcgctttttt gttggaacac ctgatatttt gcgccgttat
    10861 gccgtctgaa gccgaaacgc ggcaaacctt tatcgtccaa tccggagaaa ccgaaatatt
    10921 ggtggaaatc gcgcaagaca gggcattgca gctattgtcg gaatggatgg cgtttttcaa
    10981 tatcgggcaa aaccgcccgc tgccgttttt tgccaaaacc tcgcttgcca ccgccgaagc
    11041 gtttgcccaa aaacaagatt gggaagccgc cctgaaaaaa gcccaaaccg cctaccacgg
    11101 caacaaagtc agcaaagggc agaaagacta taccgaagtc gccctcgtgt tcggcaacgc
    11161 aagccaaaac ccgctcgaac agcccctgtt tgaaaacctc gtccgtctgc ttgccgacac
    11221 gcttgccgca gcggaaaaaa aggaagaggc cggagaagcc tgacgggatg gaaaggcaaa
    11281 tgccgtctga aaacagcaaa aaggttttca gacggcattt tgtttatcgt gtttaaggtg
    11341 cgggatagaa tgtgggcgcg ccgggaccga cgggcaggcc caaaacaaat acccaaatgc
    11401 agaataaggc aatccacgca atcaagaaga aagcggaata cggcaacatc atagaaatca
    11461 gcgtacccac gcccgcatct tttttgtatt tgatcaccgt cgccataatc agcccgaaat
    11521 aactcatcat cggcgtaata atattggtaa cggaatcacc gatgcggtaa gcggcttgaa
    11581 tgacttcggg cgcgtagccg gccaacatca gcatagggac gaaaatcggc gcagttaccg
    11641 cccattgcgc ggaggcggag cctatcatca gattgataaa agcacaaatt aaaataaaac
    11701 cgataaacaa cacgctgccg cccaagccga cttcttttaa gaacgtcgcc cctttaacgg
    11761 caatatattg cccaatattc gtccaattaa aaaatgcgac aaactgtgcg gcaaaaaaga
    11821 tgatgaccaa ataaagcccc agagtactca tcgattcggc catcgcatta acgacttcct
    11881 gttcgccgcg caaacttcgg gttacccggc cataaacaat gcccggcagt gcaaacaaca
    11941 agaaaataaa aacaacaatc gattttaaaa acggcgaacc ggaaaccaat cctgtttcag
    12001 gatgacgcaa aataccgtcg gcagggacga tgctccaagc caatagggcg gataaggcaa
    12061 caaacaccac gccagcccaa attaatcctt tatattccaa aggcgtgatt tcattggaat
    12121 gccgaatgtc tttttcttct tgtgacaaat ctgattgata agggcccaat tgcggttcga
    12181 cgattttttc agtaacaaaa taaccaatca aagcaatcac aaacgtactg gctaccataa
    12241 aaaaccagtt ggcttcaggg cctacgacgt agtcgggatg gatgatttgc gccgcctgtt
    12301 gggtgatgcc tgccaagagc ggatcgattg tgcctaagaa cagattggcc gaataaccgc
    12361 ccgaaacgcc ggcgaaagcc gcagccagac cggcaagcgg atggcggccg agggaatgaa
    12421 agatgatggc ggacaaaggg attaggacga catagcccaa ttcagaagcg gtattagata
    12481 aaatccctgt aaaaacaacc ataaaagtag tgagtttgcg tggagatttt gtgagcaata
    12541 agcgcattaa tgcggaaatc aagcccgatt tttccgcaat ccccacgccc aataaagaaa
    12601 ccaacaccgt tcccaacggc gcgaaaccgg tgaaattttt aacggtatgc gtcaggattt
    12661 tgatcaaacc gtcagcatcg agcaggctga caacgtgaat caaaccgtca tcggcacgtc
    12721 ctttcgcacc aacagggcgc ggatcgggga cggatagtcc gaaatacgca ccggcggcag
    12781 aggcaatcag caataacaca atgaaaataa taaaaagcgt aaccgggtgc ggcaacatat
    12841 tgcccagcca ttcgactgtg cgtaaaaatc gtccgtcccg ttgcgtatcg gtttgactca
    12901 tctgcttctc cttaaaaaaa atatattgag tgcattaaat ttttgaatgt aacatgaagt
    12961 gttttgtaag caaactaaaa taatcgtata tgtatataac cgtaatagat taaaacagaa
    13021 aatgcccaca tcgttattct cataaaggcg gtaatccgaa aacttgacgt taaagcctta
    13081 tcaggaatga ctggaactca acaaagcggt ttcccgcttt cgcggaaatg gcgggatttt
    13141 cggtttttgt ttgcaaaaat gccggagaaa aatgcagttt acattttgaa tggtattgtt
    13201 attaattgta aataaatatt ggaaggtaaa tgatatgaaa aatgaaattc aaaaaattat
    13261 ggacaaatat gatccatggc atgaagatga ttttgagtcg tatgaaaaca ttgccagaga
    13321 tgtatcgctg atgacagata aaacgttcat tgaacattat ttgttagaag tttattcaga
    13381 agaaaacgga catttcgacc aagagaatgt ccatgcaatg atagaagaaa ttaaaaatgc
    13441 aatttagtga at
//
LOCUS       DAWFJR010000048        13420 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWFJR010000048 DAWFJR010000000
VERSION     DAWFJR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13420
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.048"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(1..194)
                     /locus_tag="WGR04_001277"
                     /pseudo
     CDS             complement(1..194)
                     /locus_tag="WGR04_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(678..784)
                     /locus_tag="WGR04_001278"
                     /pseudo
     CDS             complement(678..784)
                     /locus_tag="WGR04_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1202..1972)
                     /gene="xth"
                     /locus_tag="WGR04_001279"
     CDS             complement(1202..1972)
                     /gene="xth"
                     /locus_tag="WGR04_001279"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0794827.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAEALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(2026..2313)
                     /locus_tag="WGR04_001280"
     CDS             complement(2026..2313)
                     /locus_tag="WGR04_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0794828.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLNRLRAGGLVDFTRYHRIIEYRLISEDAATILRTIRDLENKRAVWC"
     gene            2415..2648
                     /locus_tag="WGR04_001281"
     CDS             2415..2648
                     /locus_tag="WGR04_001281"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794829.1"
                     /translation="MDDCTGLYVPFPTFFANTRKGGLSDGIKLPPLPCLTLLPNGIIR
                     IVQKMPYNRRTSCPADASVPSGKQNTCLKRCFF"
     gene            complement(2781..3662)
                     /gene="nadC"
                     /locus_tag="WGR04_001282"
     CDS             complement(2781..3662)
                     /gene="nadC"
                     /locus_tag="WGR04_001282"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ0794830.1"
                     /translation="MPSENTLFSLPDTLLRPIVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLACLAFQTMDPSVRFQAEIQDGQAVRAGQTLAVVEGNACAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
     gene            complement(3738..4676)
                     /locus_tag="WGR04_001283"
     CDS             complement(3738..4676)
                     /locus_tag="WGR04_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794831.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            complement(4804..4941)
                     /locus_tag="WGR04_001284"
     CDS             complement(4804..4941)
                     /locus_tag="WGR04_001284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ0794832.1"
                     /translation="MPSAFQTVLYYAQLEYNKQGRLKTGLRYRKTDPLRSDKPLWFIV
                     D"
     gene            4981..6093
                     /gene="nadA"
                     /locus_tag="WGR04_001285"
     CDS             4981..6093
                     /gene="nadA"
                     /locus_tag="WGR04_001285"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ0794833.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDHETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNSGSCKSCAFCPWMAMNSLGGIKYALTSGHNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            6145..7653
                     /gene="nadB"
                     /locus_tag="WGR04_001286"
     CDS             6145..7653
                     /gene="nadB"
                     /locus_tag="WGR04_001286"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ0794834.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRTILSQGKPAIEWLLSQGVAFDQ
                     NNNGLHLTREGGHTCRRIAHVADYTGEAVMQSLIAQIRRCPNIRVYERQMALDIQTES
                     GAAYGLTVLDCRTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQSGERFMPHYDLRAELAPR
                     DIVARDIAAEIAKQTQDFVSLDISHQPAAFVRQHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQTVPSQRSSEHPSAEAGIFSDGIQSAFSRPVLQAFNQRHLGILRNDTGLHR
                     AIAQLRLWKQNQTAPHTASEYENRNLIECSLAVAQAAYARRQNIGAHFNSDC"
     gene            complement(7829..8494)
                     /gene="pgmB"
                     /locus_tag="WGR04_001287"
     CDS             complement(7829..8494)
                     /gene="pgmB"
                     /locus_tag="WGR04_001287"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ0794835.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYVEMIQAVKPEDVYPGVLPLLEA
                     LRANGKKIALASASKNGPFLLERMGLTHFFDAIADPAAVAHSKPAPDIFLAAAEGVDA
                     DIRQCIGIEDAAAGIAAIKAAGALPIGVGKAEDLGNDIALVSGTAGLTYAYLQNVWAQ
                     SGR"
     gene            complement(8506..10764)
                     /locus_tag="WGR04_001288"
     CDS             complement(8506..10764)
                     /locus_tag="WGR04_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ0794836.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFDVCKFLSVAQKELALIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGRQTDWQVSNSFEAEVGGTPETFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKIAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDESYLAKEGLE
                     VLVEVSRFWADRVHFSKRRGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNMDEKRRNFDFYEPMTVHESSLSPC
                     IHAILAAELGKEEKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGTLSFAPFLPSAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLELQVYGKDI
                     TLNGSHTVTLEK"
     gene            complement(10997..12016)
                     /locus_tag="WGR04_001289"
     CDS             complement(10997..12016)
                     /locus_tag="WGR04_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ0794837.1"
                     /translation="MSDTPTTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQ
                     EFSVLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEAN
                     EGRNALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDR
                     LTVSYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDL
                     EVFDFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDR
                     NGLVIFTAAPQDFARHDAGVYDALATEAQTLPDSLHWPEFGNIRLNKGDIREATIAYG
                     IESLS"
     gene            complement(12020..13375)
                     /locus_tag="WGR04_001290"
     CDS             complement(12020..13375)
                     /locus_tag="WGR04_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ0794838.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHSLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWIELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIHNQYALILSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFILFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
BASE COUNT         3000 a         3659 c         3710 g         3051 t
ORIGIN      
        1 tcgggcagca tcgccatcag cgtagccgtc gttatcgaac cgatgccttt gatttgctcc
       61 gccacttggg ctttgccgtc aaaatgcgtg tgggtgtggt cgtcgatttg tttgtccaat
      121 tcgtcaatca gccggtcaaa atgggcaatc agttgtttga cgcttccgac ttgcgtttca
      181 tgaaactgat gcagatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
      241 cagtacaaat agtacggcaa cgctgtactg gtttaaattt aatccactat attttgtaat
      301 agctgccatt atgcctacta ggatatatac cttctatata atccttattt aaataaaaac
      361 ccttataaaa atctgtgtta tctgtcggga ttgaaaaaaa gattttatct ttctcatcag
      421 gtttatattt gctatcaata tcaaaaagag aataaggatt acaatgagtt cgacatacta
      481 ggtaaaaatt tatatcttgg atcatgaaat attcgatatt tatcaggatc gaaagatgct
      541 aaaaattcat caatattggt actggatatt ttgtcatcac taataaacat cgctagagat
      601 gggcgacttt gattttccat ttgttgatta gtaaaggata taaatactaa cttgccattg
      661 tcaaaccaca tagatttata tagtggatta aatttaaacc agtacgtcgt tgcctcgcct
      721 tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
      781 tatagttatt gcctttggga gaatcaattc tgacagcaca taacatagat ttatctttac
      841 agtcgttata ttgagtttca tcaaactcaa ctgcatagca caataaaggc aatagaaaaa
      901 aaaggagatt aatttattac tatacataag gcagcctgac tattcattct aaaataaatc
      961 cggtttttaa tatttgattc tgctccggat tatatacata ataaatttta ctgctccaaa
     1021 atctgatatt aaccctatgt atttcataaa tttttcagaa aaataggctg tgcttgaaca
     1081 atccgccgac tccggctgtt tgtcagggaa aaggcaatta ttttcggtta gtcagcagcc
     1141 tatcctattg aatcacttaa agatatggat gtttgtcaac gccatatcaa aacacggtct
     1201 tttaccaatc gaattctgcc gtcaccggcg cgtggtcgct cggacgctcc agcgcgcgcg
     1261 tctccaaatc gacacggaca tccttcaacg cctccgccat cgcaggcgac accaaaatat
     1321 ggtcgatacg caggcccagt ttgcgttgga acatcgcgcc gcgatagtcg aaccatgtgt
     1381 aaaacgcgcc ttcgggatgg acttggcgca ggctgtcggt cagtcccaaa tccagcaggt
     1441 tttgaaacca ctgccgttcg acggacgaac agtggatttt ttcgtgccat ttttcagggt
     1501 cgtaacagtc cgcatcggca ggcgcgatat tgaaatcgcc cagtagcacc agtttgccgt
     1561 agcgggtcat ttcatcgcgg acaaactccg tcagtgcggc aaaccattgt tctttatatt
     1621 tgaatttcgg gctgtcaagg gcttcgccgt tgacgcaata gacattgatg acgcgcacac
     1681 cgccgacggt tgccgcaatc acgcgccgtt gcggatcgtc cggcagcgcg ggcagcccga
     1741 tatgcacgtc ttccggcgcg ttgcggctga cgattgccac gccgttgtag gttttctgcc
     1801 cgctccaaac acagtgccag cccatcattt gcaaagcggc ggccggaaat ttgtcctgat
     1861 cgagtttgag ttcctgcaaa accaaaatat cgggcggatt gtcggcaagc aggttttgca
     1921 cctgcggcag ccgcacattg agcgaattga cgttccaagt ggtgattttc ataacatttc
     1981 cgaaaaatgc cgtctgaaac gttcagacgg caaaaggaaa ggattttaac accataccgc
     2041 gcgtttgttt tccaaatcgc ggattgtccg cagaatcgtt gccgcgtctt cggaaatcag
     2101 gcggtattcg ataatgcggt ggtaacgcgt aaagtccacc agaccgcccg ccctcaggcg
     2161 gttcaaatgg ccggaaacgg cggtggccgg cagggataag gattttgcca gttcggcgat
     2221 attgcgttcg ctgtccaaca gttggatcag tatcgccata cgttcgggat gcgccataag
     2281 tttgagtatg gtgtggagcg gtattgtgtt catcggtatt cctaaaacct ttgccgcccg
     2341 ccaagcggat tgacgggcga cagatatgga tgattgtgat tatctttata aatcgtatta
     2401 taaatcaaat atttatggat gattgtacag gattgtatgt tccttttccc actttttttg
     2461 caaacacgcg taaaggcggg ctttcagacg gcataaaact gccgccccta ccctgcctca
     2521 ccctgttgcc gaacggaatc atccgcattg ttcaaaagat gccttacaac cgccgcacgt
     2581 cttgccctgc cgatgcctcc gtgccttcgg gcaaacaaaa tacctgcctg aaacgctgtt
     2641 ttttctaaag catcacgacc ttcacgaaaa atgccgtctg aagaatattt cagacggcat
     2701 ttcctccttg aggcaataac cggattacgg ttaggcggtt ccttgtctga catatcagac
     2761 aactgccgac accctaaaac tcatgccaag aaatccaaac ctatgtccaa cgagcggctg
     2821 ctgtgggtca gatagccgag ggcgatgccg tccacgccgg tttgcgccac gcgcttcagg
     2881 cggtcgaagc cgatgccgcc cgatgcttcg caatagacgg tgtgggggtg ggcggtttgc
     2941 gtgtggcagc ggtttgccgc ttctttcagg gtttcgtcgt ccatgttgtc cagcaaaatc
     3001 cgttccgcgc ccgctgcgat ggcttcgtcc agttgtgcca acgtgtccac ttcgatttcc
     3061 acgcaggtca atgctccgac cgcctgtttt gcctgccgaa cggcttgggc gatgctgccg
     3121 caataggcga ggtggttgtc tttgatgagc acggcgtcgt ccaagcccat gcggtggttc
     3181 acgccgccgc ctgccctgac ggcgtatttt tgcaggacgc gcagcagggg gatggttttg
     3241 cggctgcaca cgatgtctgt accgtattcg gcgacttcgg caacggcacg cgcggtggcg
     3301 gtggcgatgc cgcttaagtg cgtgaggtag ttgagtgcgg tgcgttcggc ggcgagcagc
     3361 gcgcaggcgt tgccttcgac gacggccagc gtctgacctg cgcggacggc ttgtccgtct
     3421 tggatttcgg cttggaaacg gacggacgga tccatcgtct gaaaggcgag acacgccaag
     3481 tccataccgg cgataacacc gtcttcgcgg ctgacgagga agagtttggc ggttttgtcg
     3541 ggggcgataa cggcggcgga cgtaatgtcg ccgcgcctgc ccaagtcttc gctcaaggct
     3601 tgttcgacta tggggcgcaa cagggtgtcg ggcagggaaa agagggtgtt ttcagacggc
     3661 atgaggtttc ctttgtcgaa aaagattgat gttgcaccga tggggcaaaa cttcatttga
     3721 tgccgtctga aaacgggcta acggctgccc agcggcagtc cgatgtccga aatcagcctg
     3781 tcgggcagga cgtcgtcgcg gaagcggcaa agctgcgcgg gacggccttt gctgcccgat
     3841 acgccggtat ccgacggctc gatgaggttt tgctgctgaa tctggcggcg gaagttttgc
     3901 ttgtgcagca atcttccgct gatggcttcg acactgtttt gcagttgcag cagcgtgaat
     3961 tcgggcggca tcagctcaaa aatcacgggg cggtatttga ttttggcgcg caggcgggac
     4021 agggcggtcg ccaatacgcg gcggtggtcg tggcgcatgg gctgccctgt gagcgcgaaa
     4081 tcgaagtttg cctgcggctc ggcggcttcc actatcaggc cgctttcata cagcatttca
     4141 tagcgttgca aaacgtattc ttccgaccag ttttccggtt cgacccccca acacaaatga
     4201 atgcgcttga gccgcatttg acgcacttcc tccgtgtccg ccgagtttgc ccaaatgcgc
     4261 agtcggctga cgacggcgtc gcgctgtccg ccgtcggtgc gcaagtcttc ccacgggaaa
     4321 tagccgtagc agtcctgcca tttcgcatcg ggatgcagga tgctgtcggc tgcctcgcgc
     4381 accagcccca aatagctgac gtacaagacg ggcatgccgt gttcgttgcg gcggtgggta
     4441 tcgacaaagg tgtaaagctg ttccacatag cccataggct gcgaagtctg cttggcaacc
     4501 cacagcttca cgcccgcctg caaggaattg cgcagagggg agagcgggcc gttgggcagg
     4561 agtgtgcctt gggcgacggt taatacccgc aggccgccgt cggtaacggc aatcaatacg
     4621 ggaaccagct cgacgatgct ttgcggcggg gcttcggctt cggggtaggc atccatgctc
     4681 ggctttctgc gggtgggaaa ggctttattg tatagtggat taaatttaaa ccagtacagc
     4741 gttgcctcgc cttgccgtac tatttgtact gtctgcggct tcgtcgcctt gtcctgattt
     4801 ttgttaatcc actataaacc aaagcggttt gtcggaacga agcgggtcgg ttttacggta
     4861 tcgcaaacct gttttcagac gaccttgctt attatactca agttgagcat aatataaaac
     4921 agtttgaaaa gcagacggca aaccttcatg ccgtctgaaa accagctaca aggaaacatc
     4981 atgcaaaccg ccgcccgccg ctcgttcgac tacgatatgc ccctcatcca aacgccgact
     5041 tccgcctgcc aaatccgtca ggcgtgggcg aaggttgccg atacgcccga ccacgagacg
     5101 gcaggccgtc tgaaagacga aatcaaggct ttgctgaagg agaaaaacgc ggtcttggtg
     5161 gcgcattatt acgtcgatcc gctgattcag gatttggctt tggaaacggg cggatgcgtg
     5221 ggcgattcgc tggaaatggc gcgcttcggt gcggaacacg aagccggtac gctggtggtg
     5281 gctggtgtgc gcttcatggg cgagagcgcg aaaatcctct gccctgaaaa aacggtgctg
     5341 atgcctgatt tggaggcgga atgttctttg gatttgggtt gtcccgaaga agcgttttca
     5401 gcgttttgcg accaacaccc cgaccgcacg gtggtggtgt acgccaacac ttccgccgcc
     5461 gtgaaagcgc gtgccgactg ggtggtaacg tcttcggtgg cgttggaaat cgtgtcgtat
     5521 ttgaaatcgc gcggcgagaa actgatttgg ggacccgacc gccacctcgg cgactacatc
     5581 cgccgcgaaa cgggcgcgga tatgctgttg tggcagggtt cgtgcatcgt ccataacgaa
     5641 ttcaaagggc aagagctggc ggcgttgaag gcggaacatc ccgacgcggt ggtgctggtt
     5701 catcccgaat cgccgcaaag cgtcatcgaa ctgggcgacg tggtcggctc gaccagcaaa
     5761 ctgctcaaag ccgccgtatc gcgtcctgaa aaaaaattca tcgtggcgac cgatttaggc
     5821 atcctgcacg aaatgcaaaa gcaggcgccc gacaaagaat ttatcgccgc accgacggcg
     5881 ggcaacagcg gaagctgcaa aagctgcgcg ttctgcccgt ggatggcgat gaattcgctg
     5941 ggcggcatca aatacgccct gacaagcggg cacaacgaaa tcctgttgga cagaaagctg
     6001 ggcgaagccg ccaaactgcc tttacagcgt atgctcgact tcgcggcagg actcaaacgc
     6061 ggggatgtgt tcaacggcat gggccccgcc tgatttgccg tcaatattgc cgtttcagac
     6121 gacctctcaa acaaggacaa caccatgcaa accgattgcg acgtattgat tgccggaaac
     6181 gggctggcgg cactgacgct cgccctgtcg ctgcctgaat cgttccgcat cgtcattttg
     6241 tgcaaaaacc ggctggacga caccgccagc cgtcatgcgc aaggcgggat tgcggcggcg
     6301 tggtcgggag aggacgacat cgaaaaacac gttgccgata ccttggaagc tggcgcgggt
     6361 ttgtgcgacg aagccgccgt ccgcaccatc ctgtcgcagg gcaaaccggc aatcgaatgg
     6421 ctgctgtcgc agggcgtggc gttcgaccaa aataataacg gcctgcacct gacacgcgaa
     6481 ggcgggcata cctgccgccg aatcgcccac gtcgccgatt acacgggcga agccgtcatg
     6541 cagagcctga ttgcccaaat acgccgctgc ccgaacatcc gcgtttacga gcggcagatg
     6601 gcgttggaca tccaaaccga atcaggcgca gcgtacggac tgaccgtcct cgactgccga
     6661 acgcaagaaa cctaccgcat ccgcgcccgc cataccgtac tcgcaggcgg cggcttggga
     6721 cagatttacg ccgccaccac cacgccgccc gaatgcacgg gcgacgccat cgccatggca
     6781 atacgcgcag gctgcgcagt cgaaaacctc gaatttatcc aattccaccc cacaggcttg
     6841 gcaaggccgt ctgaaaacgg acgcaccttc ctgatttccg aagccgtgcg cggcgaaggc
     6901 ggcattctga ccaaccaatc gggcgaacgg tttatgccgc attacgacct ccgtgccgaa
     6961 ctcgcaccgc gcgacatcgt tgcccgcgac atcgccgccg aaattgccaa acaaacgcaa
     7021 gacttcgtct cgctcgatat cagccatcaa cccgcagcgt tcgtccgtca gcatttcccg
     7081 tccatccatc ggcactgcct gtcccaatgc ggcttggaca tcacgcgcca agccatcccc
     7141 gtccgccccg tgcaacacta cacctgcggc ggcatccaaa ccgacccttg cggcagaacc
     7201 tccctgccgc agctctacgc cttaggcgaa accgcctgca cagggctgca cggagccaac
     7261 cgcctcgcca gcaattccct gctcgaatgc gtcgttaccg ccagacttgc cgcccaaacc
     7321 atcgcggacg gacaagcatt ccaaaccgta ccgtcccaaa ggtcgtctga acacccctcc
     7381 gccgaagcag gcatcttttc agacggcatc caaagcgcat tcagccgccc cgtcctgcaa
     7441 gccttcaacc aacgccatct gggcatcctc cgcaacgata ccggcctgca ccgcgccatc
     7501 gcccaactgc ggctttggaa gcaaaaccaa accgcgccgc acaccgcgtc cgaatacgaa
     7561 aaccgcaacc tgatagaatg cagcctcgcc gtcgcccaag ccgcatacgc aaggcggcaa
     7621 aacatcggcg cgcattttaa tagtgattgt taaggttggc aggtcagaga aaatcgttca
     7681 aataaatttg aatagcatgg ttaagaaatt ggcattcggt ttttagggtt gcgtaagttt
     7741 ggttatcatt ctgttctaaa agcaaaaagg ccgtctgaaa cactgttcag acggtctttt
     7801 gcttccttga cactttatct gacgcgtttt acctgcccga ctgtgcccac acgttttgca
     7861 ggtaggcgta ggtcagcccg gcggtgccgg acaccagcgc gatgtcgttg cccaaatctt
     7921 cggctttgcc cacgccgatg ggcagggcac cggcggcttt gatggcggcg atacctgcgg
     7981 cggcgtcttc aatgccgatg cattggcgga tgtctgcgtc cacaccctcg gctgcggcga
     8041 ggaagatgtc gggggcaggt ttggaatgcg cgacggcggc agggtcggca atggcgtcga
     8101 agaagtgggt cagccccatg cgttcgagca ggaacgggcc gtttttactg gcggacgcga
     8161 gggcgatttt tttgccgttt gccctcaatg cttccagcag cggcaaaacg ccggggtaca
     8221 cgtcttcggg tttgaccgcc tgaatcatct cgacgtagtt gtcgtttttg cggcgggtca
     8281 gttcggcaaa ctcggcttcg ccgacggttt tgccgccgtg cgcgaggatg cgtttgagcg
     8341 aatcgtcgcg cgacacgcct ttgagctgct cgttaaactt gcggtcgatg ccgatgccca
     8401 gttcttcggc gagctttttc catgcgcggt agtggtattc ggcggtgtcg gtgatgacgc
     8461 cgtcgagatc gaagagcact gcggtaaacg tcattttgcg cccccttatt tttccaacgt
     8521 gacggtatgg ctgccgttga gcgtgatgtc tttgccgtac acctgcaatt cgagcggttc
     8581 gcctttgagc agggtgaaga cgacgttttc tttgccgacg gcgactttaa tcagacggcc
     8641 tcggtagttg atgtggaagg cgtagcctgt ccacgcactc ggcaggaacg gtgcgaagct
     8701 gagtgtgccg ccccaagttt tcatttgggc gaaaccttgg acgatggcga gccacgagcc
     8761 ggtcatggag gtaatgtgca ggccgtcttc ggtgtcgtta ttgtagttgt ccaagtccag
     8821 acgggcggtg cgctggtaca tttccacggc tttttcttct ttgcccagtt cggcggcaag
     8881 gatggcatga atacacggcg acaacgagct ttcatgcacg gtcatcggtt cgtagaagtc
     8941 gaagttgcga cgtttttcgt ccatattgaa acggtcgccg aagaagtaga tgccttgcaa
     9001 gacgtccgcc tgtttgatga agggcgaacg caggattttg tcccacgacc atttctggtt
     9061 gagcggcaaa tcgtcgggcg aaagcgcgga cactgggcgg atgtctttat caaggaagcc
     9121 gtcgtgttgg acgaatacgc cgagttcttc gtcatgagga cggtacatat tcgcgctgat
     9181 gtccgcccat ttttccaact cggcggcacg cacgttcaaa tccggacgcg ggtatttcgc
     9241 caaggcttcg cgggtgtagt ccaataccca agcggcaagg gtgttggtgt accagttgtt
     9301 gttgatgttg ttttcgtatt cgttcgggcc ggttacgccg tgaatcatgt atttacccct
     9361 gcgtttggag aagtggacgc ggtccgccca gaagcgggac acttcgacca agacttccaa
     9421 gccttctttg gcaagatagc tttcgtcgcc ggtgtagttg gtgtagttgt agatggcgta
     9481 aggaatcgcg ccgttgcggt ggatttcctc gaaggtgatt tcccattcgt tgtggcactc
     9541 gatgcccgta aacgttacca tcggatagag tgcgcccgcc aagccctgtt cgcgcgcgtt
     9601 gtgctgcgcc tgcggcagtt ggttgcggcg gtattgcagc aggttgcggg taacttcggg
     9661 ttccgccagt gcgaggtaaa gcggcacggc gtaggcttcg gtgtcccaat aggtcgcgcc
     9721 gccgtatttt tcgccggtaa agcctttcgg gccgatgttc aggcgcgcgt cttcgccgta
     9781 gtaggtggag aacagttgga acaggttgaa gcggatgccc tgctgcgctt cgtcgctgcc
     9841 ttcgatgacc acgtcggcga tttcccaacg gtgcagccag cctgctttgt gcgcgtccag
     9901 caaggtttca aacgcaacgc ctgcgatttt ttccgacaag gcgcggcctg cggctttcac
     9961 tgcttccaag ccctgataat cgcggctggt ggtaacaatc acgcgttttt caaaggtttc
    10021 gggcgtgcca ccgacttcgg cttcaaaaga attggagacc tgccagtcgg tttgtctgcc
    10081 gccgagggct ttaaagctgc cggcgaaggt ttgctcggcg ttgacgataa attgttccac
    10141 gccgaaaggg ttggcaacgg tttgggcggc aatgtaggag agactgtctg aaacgccttt
    10201 gtccaatacc tgccagaatt tttcttcgta gttggagtct tcgtttttca cgtcggcatc
    10261 gatgatggaa tcgatgcgga cttggtgggt tttgccgtca acggatacgg cttcccagcg
    10321 gatgagagcc agctcttttt gtgcgacgga caggaatttg cacacatcga aacgcacgcc
    10381 gaatacggtg aacgagcggc gcaacacgcc gtgctgcata tcgagttcga cggagaagcc
    10441 ggcaacatcg tttttcgcca agtccacctc ttgaccatcg acaaagattt tgactttgct
    10501 gaaattgagc gcgttgatgg ctttgccgaa atatttgggg tagccgtttt tccaccagcc
    10561 gacgcgggtt ttgtcgggaa accacacacc ggcgatgtag gtacccaagt ggctgtcggc
    10621 ggaataggtt tcctcaaagc tgccgcgcat acccatatag ccgttgccca aactggtcag
    10681 gctctcttgc agccgtttgt gttctttttc cagctttgcc gaacgcagcg tccaagggct
    10741 gatttccata attcttgtgt acatttatga agctcctgtt tggattgatt tgagggaatg
    10801 gtgaaatctt atagtggatt aacaaaaatc aggacaaggc gacgaaccgc agacagtaca
    10861 aatagtacgg aaccgattca cttggtgctt cagcacctta gagaatcgtt ctctttgagt
    10921 taaggcgagg caacgccgta ctggtttttg ttaatccact ataaaaaggt cgtctgcaac
    10981 cggtgttagg aagctcctaa gaaagggatt cgatgccgta agcaatcgtc gcctccctga
    11041 tatcaccctt gttcagacga atattgccga actcgggcca atgcaggctg tcgggcagcg
    11101 tctgcgcctc ggtcgccagc gcgtcgtaaa cgcccgcatc gtgccgcgcg aaatcctgcg
    11161 gggcggcggt aaagatgacc aagccgttgc ggtcgctgta tatgctgata cgacggcggc
    11221 gtccggcttg caacacagcg gcgggacggc ctatatcgga cggcacgcgg taagcgtcgt
    11281 caaaaccggc ccgacccgtt tcgcggcgca gggcggcaac ggcggcatcc agcggcttgg
    11341 gccggctgaa atcaaatact tcgaggtcgt ctgaaaccgt tgagacgggc agtttttcgg
    11401 catcggccgg aatatgtccg ccctgcggaa tatgcagaac cgcatcgtgc aggcccgcgt
    11461 ccagccgcca gtaaatgtgc agcgtcgggt cgaacaccgt gtcgccgagc gcggtggcgc
    11521 gatagctaac ggtaagccgg tcgtcctcgt ccaagcggta ggaaatatcc aaatccaaat
    11581 cgttgggata accgtcggcc gactgttgca ggcggctgcg cagcaccacc gaacggccgt
    11641 ctgccgccac cgcgttgaaa cgggtaacgg ccagcccgtg cgaaccgccg tgcagcgcgt
    11701 tcctgccttc gttggcctcc acgcggtaag tcctgccgtt gatgtcgaac gccgcaccgc
    11761 ggatgcgtcc ggccacgcgc cctatctgtt tgttaatctg aaacggattg tccgcatagg
    11821 aagccgcatc gtcgaacgac accacgaggt tttcgcgcac gccgtctgcc aaaacggaaa
    11881 attcctgcac aatcccgccc aagtccagca cgcagacgcg cgtaccacgc cgattggaca
    11941 gcacatagcc ggttacggca cgcccgtcga tcaggccgaa atcgcgggta gcgggggtag
    12001 tgggggtatc gctcatcgct caaaccccgc cgtgtgtttc tttaatcagg aacacggaaa
    12061 acgcgcccag cagcaggacg acgcccccta ccaagaacat agtggcctgc aagccgccca
    12121 gcatcgggaa caatatgaag ctcaacagcg aagcgacgat ttgcggcata cagatagagc
    12181 cgttaaacag gcccaaataa gtacccatgt gtttgccgga gagggcattg gttacaatcg
    12241 tcaaaggata ggtaataatg cctgcccacg cgatgccgat taaggtataa gacaggatta
    12301 atgcgtattg attgtggatg aagaaaacgg agaaaaagcc gagcgcgccc aaagccaaac
    12361 agccgaaata acccgcctta tggtatttat tcggcacttt cgccaataca aacgaacaaa
    12421 tcaccgccgc aaccgactgc accgccgcca aaacgccgta ccagttaccc gcctcctgat
    12481 aacctacgga agacgcatcg gtggtgtgcc agacgttttc cgcaatcgcg cctgccgagt
    12541 aagtccacat atattggaag gcgaaccagc agaagaattg caccaaagta accgtccaaa
    12601 acgccttagg cgcggttttc aagagttcga tccagttggc tttttcctga ttcgcggcga
    12661 catcgatgcc gtggtaacgg gcgtaggttt ccggatcgta ttccttcact ttgaaaatcg
    12721 tgaacgcgct ggtaatcacc agcaacgccg cacccacata aaacgccacg accacggtct
    12781 gcggcacaac gcctttctcg gcggtgttcg ccaaaccgat atacgcaaac acaaacggca
    12841 gaatcgccgc cacgaccgcg cccgtattcg ctaagaaact ttgaatcccg taggcgtagc
    12901 ctttctgctc ctcgttgacc atgtcgccga ccatcatctt aaacggctgc atcgccatat
    12961 ttgacgacac gtctaacagc gcaatcatca gcgcgccgaa cgacaaagcc gccagcgacg
    13021 catagccgaa accgaagctg cccgagttcg gcatcaaaat catcacaata accgcaatca
    13081 acgtgccata aagcagatac ggcagacggc ggccgcccaa acgcggcttc caagtgcggt
    13141 cggagtaatg gccgacaatc ggctgcacca gcatccccgc cagcggcggc aggataaaga
    13201 accagccgag gctgtgcggg tctgcgccta gcgtttgaaa aatgcggctc atttgcgagc
    13261 tttgcagggt aaaggccgtc tgaacgccga gaaagccgaa actgagcatc caaatcgtgc
    13321 tttttgccag cgcgggcaaa ccttgttttg ctgtttgagg cgtatattcc gacataaggt
    13381 aaatcctttc tttgatttga aaagtatagt agattaaatt
//
LOCUS       DAWFJR010000049        13331 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWFJR010000049 DAWFJR010000000
VERSION     DAWFJR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13331
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.049"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(305..1201)
                     /gene="argB"
                     /locus_tag="WGR04_001291"
     CDS             complement(305..1201)
                     /gene="argB"
                     /locus_tag="WGR04_001291"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ0794839.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKAGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRLPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1371..2501
                     /locus_tag="WGR04_001292"
     CDS             1371..2501
                     /locus_tag="WGR04_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ0794840.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPCQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2572..3423
                     /locus_tag="WGR04_001293"
     CDS             2572..3423
                     /locus_tag="WGR04_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ0794841.1"
                     /translation="MIIHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDLEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     LGMKKQH"
     gene            complement(3506..4168)
                     /locus_tag="WGR04_001294"
     CDS             complement(3506..4168)
                     /locus_tag="WGR04_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ0794842.1"
                     /translation="MDSQKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAADDEEFEELFYPIMALGGIYDEEENGAIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            4202..4309
                     /locus_tag="WGR04_001295"
                     /pseudo
     CDS             4202..4309
                     /locus_tag="WGR04_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4368..6161)
                     /locus_tag="WGR04_001296"
     CDS             complement(4368..6161)
                     /locus_tag="WGR04_001296"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ0794843.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            6406..7515
                     /gene="proB"
                     /locus_tag="WGR04_001297"
     CDS             6406..7515
                     /gene="proB"
                     /locus_tag="WGR04_001297"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ0794844.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDALAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            7528..8790
                     /locus_tag="WGR04_001298"
     CDS             7528..8790
                     /locus_tag="WGR04_001298"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ0794845.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDGKRICAMADGIRAVTALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGRESSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIETHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
     gene            complement(9015..12047)
                     /locus_tag="WGR04_001299"
     CDS             complement(9015..12047)
                     /locus_tag="WGR04_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEZ0794846.1"
                     /translation="MFWIVMIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPNASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNGAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIYIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPTGFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSNGQYLSETEAFGHDSQAICPFEDVPSERPSCRVSDTEADE
                     GAFPSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGGGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            12211..12891
                     /locus_tag="WGR04_001300"
     CDS             12211..12891
                     /locus_tag="WGR04_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ0794847.1"
                     /translation="MIFQTVWFSDVVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLSYCLLFA
                     GSAGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            12920..13279
                     /locus_tag="WGR04_001301"
     CDS             12920..13279
                     /locus_tag="WGR04_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ0794848.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPASLSPATGNLFANWTGA
                     LLIGIFAETVNHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGTAVK"
BASE COUNT         2921 a         3809 c         3450 g         3151 t
ORIGIN      
        1 aaagaaacct tttttcccga taagtttccg tgccgacagg tctagattcc cgcctgcgcg
       61 ggaatgacga aatttcaaag ttatggcgtt atcggaaaaa caaaaaatca aagccggaga
      121 atttatccca aacaaccgga tttcaaaaaa accagattcc cgccttcgcg ggaatgacgg
      181 attttaggtt tctgttttgg tttctgtttt cgcgggaata acaacttaga aattgcccga
      241 aaccccaaaa acagaaaccg gacaaacggg ttttccgcca aagccggcat tttccgactt
      301 tgcttcaggc atcttcccca ccgcccaaaa tcatcgaacc gataccggca tcggtaaaga
      361 tttccagcaa aagcgcgttg ggcaacctgc cgtcgatgat atgcgtggct ttcacaccgt
      421 tgacggcggc ttcgaccgca gaagcgattt tcggcagcat accgccatac agcgtgccgt
      481 cggcaatcag ttcatcaatc cgtttcggcg tgagtttggt cagcagattg cccgttttgt
      541 ccatcacacc ggcgatattc gtcatcatca agagtttttc agcgttcaat tcttccgcca
      601 atttgcctgc caccaaatcg gcgttgatgt tgaacgcttc gcctttttca cctacgccga
      661 cgggggcgac gacgggaatg cagccgcgtt ctatcagccc tttaaccaaa ccggtatcga
      721 tgctttccac cgtaccgacc tgtccgatgt ccacgccatt ctgttcgggc gtatcgatca
      781 aaagtttctt cgccttaatg aaatgatcgt cgcgtccgct cacgccgacc gcgtgtccgc
      841 catatgtgtt aatcatcgac acgatttctt tattgacatg cccgcccaac accatttcga
      901 caatatccat cgcctctttg tcggtaacgc gcattccttg gacaaactca ccctttttgc
      961 cgactttttc aagcatcgca ttgatctgcg gcccgccgcc gtgaacgatg acgggatgaa
     1021 tgccgaccag cttcagcagc acgacatcgc gggcaaaccc tgctttcaag gcaggttcgg
     1081 tcatcgcgtt gccgccgtac ttgatgacgg cgaccgaacc ggaaaaccgg cggatgtaag
     1141 gcagcgcttc ggcaaggata cgcgctttgt cggcggcgga aataatgttt tcagactcca
     1201 taacgactcc ctattgttga caatttcggc ttatgttaac gccaaacgcc gtctgaaaca
     1261 atcttcagac ggcatccggc agaccttcgg gcaggttgaa gccttgcgat acgggcggta
     1321 aaatcgcggt ttatccggtt cgagaaaccg gcgacagcaa ggactttccc atgattaaaa
     1381 tcagcacccg cttcgatgcc ggctcggtcg tcgtcaaaga cctgaccgat ccttccaaca
     1441 tccgcctcgc cctgcgtccc gacaacgcct ccgattttgc acaatggttc tacttccggc
     1501 tgcaaggcgc ggcatatcaa aactgcatca tgcattttga aaacgcggca gaagccgcct
     1561 acccgaaagg ctgggaaggc tatcaggcgt gtgcctcata cgaccgccgc aactggttcc
     1621 gcgtaccgac ttcctacgaa aacggcgtac tgaccgtcaa tcatacgccg ctgtccaaca
     1681 gcgtgtatta cgcctatttc gaaccttact ccgaagaaca gcatttaaac ctcctcggcg
     1741 acgcgcaagg cagcggcctg tgccgcatcg acgacttggg cagcaccgtg caagggcgcg
     1801 acatcaatct gctgaccatc ggcaatcagg tcgaaagcga tctgaaaatc tggattaccg
     1861 cccgccagca tccgggcgaa accatggcgg aatggtttgt cgaggggctg ctcggcaggc
     1921 tgctcgatcc gcaagacccg acagcgcgca ccctgctcga ccgcgccacg ttctatatcg
     1981 tccccaatat gaaccccgac ggttcggcac tgggcaacct gcgtaccaac gccgccggtg
     2041 cgaacctcaa ccgcgaatgg gaaaatccga ctttggaaaa aagccccgaa gtgttcttcg
     2101 tgcgcgaaaa aatgctggaa accggcgtgg atttgttctt ggacatccac ggcgacgaag
     2161 gactgccctt tgtctttgtt gcaggtacgg aaggcgtgcc aaactacaat ccgcgcattt
     2221 ccgcgttgga aacacagttc aaaaccgccc ttttgaacgc cagccccgat ttccaagacg
     2281 aatacggtta cgaaaaagat gcgccctgtc aggcaaacat gaccttggca acaaactggg
     2341 tcggcaaccg tttcaactgc cttgcctata cgttggaaat gccgttcaaa gacaatgcca
     2401 acctgcccga cgacgatttc ggatggaacg gtcaacggtc gttgcgcttg ggcgaagccg
     2461 cgttgtccgc catcctcaac atcatcggcg atttgcgctg aaacccttat tgcagaaatg
     2521 ccgtctgaag catcatgcct ttcagacggc atttgaaaac gaaagcctgt tatgattatc
     2581 catccccaat tcgatcccgt cctcatcagt atcggcccgc ttgccgtccg ctggtatgcc
     2641 ctaagctaca tcctcggatt tattcttttt acctttctcg gcagaaggcg catcgcgcaa
     2701 ggcttgtccg tttttaccaa agaatcgctc gacgacttcc tgacatgggg cattttgggc
     2761 gtgattttgg gcgggcgttt gggttacgtc ctgttttaca agttttccga ctacctcgcc
     2821 catccgcttg atattttcaa ggtatgggaa ggcggaatgt cgttccacgg cggctttttg
     2881 ggtgtagtta ttgccatatg gttgttcggc cgcaaacacg gcatcggctt cctcaaactg
     2941 atggacacgg tcgcaccgct cgttccgctg ggtctcgctt cgggacgtat cggcaacttc
     3001 atcaacggcg aactttgggg acgcgttacc gacatcaacg cattttgggc aatgggcttc
     3061 ccgcaggcgc gttacgaaga cctcgaagcc gccgcgcaca atccgctttg ggcagaatgg
     3121 ctgcaacaat acggtatgct gccgcgtcat ccctcgcagc tttatcagtt tgcacttgaa
     3181 ggcatctgcc tgttcgccgt cgtttggctg ttctctaaaa aacagcggcc gaccggacaa
     3241 gtcgcctcgc tcttcctcgg cggctacggc atattccgct tcattgccga attcgcacgc
     3301 caacccgacg actatctcgg gctgctgacc ttggggctgt cgatggggca atggttgagc
     3361 gtcccgatga ttgttttggg tatcgtcggc tttgtccggc tcggcatgaa aaaacagcac
     3421 tgaccgcctg tgaaaaatac cgtctgaaac atggaaatcc ggtttcagac ggtattttta
     3481 cgtttggttc atggaaacaa acgcttcaat tcttaccgca acacgccttg tattttctgc
     3541 cgctgccgca cggacaggga tcgttcctgc cggttttttc cccttccctg cggacggttt
     3601 gcggcttgtt gatgaccgcc tgccagtagc ggtaaatgtc cgccagcgcg taaggcaact
     3661 cggattccag ctccgccagc tcgccttctg tgaattgcag ccggatagcg ccgttttcct
     3721 cttcgtcgta aataccgccc aatgccatga tgggataaaa caactcttca aattcttcat
     3781 catcggcggc ttcaaaccaa tcggtcggca caatatccaa gccgtaaaga taagcattgc
     3841 accatgtgta aaaatcgctg ccgccgtctt cgttttcata cagccacaaa tcgggcagtt
     3901 ttttatccga catcgcggcg gttgtttcca tcgccattgc caaaaccagc cgttcgattt
     3961 cggaacgttc ggcggcggta aattgcgatt cgtcgcccaa cacttcgggc agccagtcga
     4021 gcggtgtcaa tttgtccggc ccgctcaaca gcgccgtcat aaaaccttga acctcgtcgc
     4081 aacgcatcgt gttgccttgt tcgcttttgg catccaacaa ttcgctcaac cgccgtttgg
     4141 atgcttcggt aaatttttgg gaatccatca ttttcctttt caaatgggtt ttgcgcccta
     4201 ttatagtgga ttaaatttaa atcaggacaa ggcgacgaag ccgcagacag tacaaatagt
     4261 acggcaaggc gaggcaacgc cgtactggtt taaatttaat ccactatatc gccgcaatgc
     4321 cgtctgaagc ctttttatgc ttcagacggc atcgtctgtt ttcacgctca aatcgtaccg
     4381 ctgccttgcg ctttcggttt ggcggcacta aattccagtt tgctcaaggc ggaaaggtag
     4441 gctttggcgg tagcgaccaa aacgtcggta tccgcgccct gaccgttgac gacgcggttg
     4501 ccgcgcgcca gacggacgct ggtttcgccc tggctttccg taccttgcgt gacggcgttg
     4561 acggaataaa tctgcaaagc cgcgccgctt tgcgccacgc tttcaatcgc tttgaaaatc
     4621 gcatccacgg ggccggagcc tgtggcggaa gcgcgttttt cttcgccttt gatgctgaac
     4681 acgatgtcgg cgcgcggctc ttcgccggtt tcggtgctga ttttttggga gatgaatttg
     4741 tagctctcgg cattcatgct gcccatttcg tcggatacca gtgcgtgcag gtcttcatcg
     4801 aagatttcgc gttttttgtc ggcgagttct ttgaagcggg caaacgcggc gttcagtgcc
     4861 tcttcgcttt ccaactcgat gcccaaatcc gccagcttgg ttttgaaggc gttgcggccg
     4921 gacaatttgc ccaagctcaa acggtttgtt gcccagccga ccgattcggc ggacataatc
     4981 tcgtaagttt cgcggtgttt cagcacccca tcctgatgga tgcccgattc atgcgaaaag
     5041 gcattggcac cgacaatggc tttgttgggc tgcacgggat agcccgtaat ggtggacacc
     5101 agtttggacg aaggcacgat ttgcgtggta tcgatgccgg tttccaagcc gaacaagtcg
     5161 tggcgcactt tcaacgccat cacgatttct tcaaccgaag cattgcctgc acgttcgccc
     5221 aagccgttga cagtacattc cacctgacgc gcaccgcctt ttaatgcggc aagcgaattg
     5281 gcaaccgcca agcccaaatc gttgtggcag tgtgccgacc aaacgacttt gccgccgttg
     5341 ggcgttttgg caatcagttc gcggaaaaat tcttcggttt tgtacgggat ggaatagccg
     5401 acggtatcgg gaatattgat ggtggtcgcg cccgcttcaa tcaccgcgcc gcagatttcg
     5461 gcaaggaaat cgatttccga acgcaacgcg tcttcgcagg aaaattccac atcgtcggtg
     5521 tattcacgag cgattttcac cgctttgacc gccgcctcaa tcacctgctt cggcttcatc
     5581 ttcaatttgt actccatatg gatggggctg gtggcgatga aggtgtggat gcgttttttc
     5641 ggcgcgggcg caacggcctc acccgcctga cggatgtccc gctcgatggc gcgggacaat
     5701 gaacagaccg ttgatttggt aatggttttc gcaatcgcat tgaccgcctc gaaatcgccc
     5761 gggctggcag cggcaaaacc cgcttcgatg atgtccacac ccaatttttc cagctggcgg
     5821 gcgacgcgga ttttttcctc tttggtcata gcggcgccgg gcgactgctc gccgtcgcgc
     5881 agggtggtgt cgaaaatgat aacgcggttg gcctgtgtca tttctgttct ctccagagat
     5941 tcgttttttt gtaaaaaagc attcaaatcc agcggcctgc cttgcgattc ggcaagcgcg
     6001 gtcagctttt gcagttgcgc caaaggcagc gacccgcgcg tgcgccattt atagatggcg
     6061 gcggtcgtcg cggcattttc gggatcgtgc tgtttcaacg cttcggcaag cgcgttcacg
     6121 ccgccgaaat aagcaaccaa gcggtctata tctaattgca tggtagtcct tgtctaaaat
     6181 aaatcagcct acagcagaaa tatggacagg attatacgca ttttggacaa aacggcaata
     6241 taaatttgaa aaatctcaat aagccgccat agattttatt aattttatac attttgtcca
     6301 ttttattgca gacgcggcgt gattgttgac aattgcggca gaacagggca aattcggcgg
     6361 cacaaggtgg caacgagaca ggacaaacca acaaggacgg aaacaatgaa atacaaaaga
     6421 atcgtattta aagtcggcac atcttcgatt acccattcgg acggcagtct ctcgcgcggc
     6481 aaaatccaaa ccatcacccg ccagcttgcc gcattgcatc atgcgggaca cgagctggtc
     6541 ttggtgtctt ccggcgcggt tgcggcaggg ttcggtgcgc tgggtttcaa aaaacgtccg
     6601 gtcaaaatcg ccgacaaaca ggcttccgcc gccgtcgggc aggggctgct gatggaagaa
     6661 tatacggcaa acctgtcttc agacggcatc gtgtccgcac aaatcctgct cagccgcgcc
     6721 gactttgccg acaaacgccg ctaccaaaat gccggcggcg cactttccgt gctgctgcaa
     6781 cgccgcgccg tccccatcat caatgaaaac gatacggttt cggttgagga attgaaaatc
     6841 ggcgacaacg acacattgag tgcgcaagtg gcggcgatga tacaggcaga cctcttggtg
     6901 ctgctgaccg acatagacgg tctttacacc ggcaacccga acagcaatcc cgatgccgta
     6961 cggctggaca aaatcgaaca catcaaccat gaaatcatcg aaatggcggg cggatcgggt
     7021 tcggcaaacg gcacgggcgg tatgctgacc aaaatcaaag cggcaaccat cgccgccgaa
     7081 tccggcgtac cggtgtatat ctgttcctcg ctcaaacccg atgcacttgc cgaagctgcc
     7141 gaacatcagg cggacggctc gtttttcgtc ccccgtgcca aaggtttgcg gacgcagaag
     7201 caatggctgg cgttctattc cgaaagccgg ggcagcgttt atgtggacga aggtgcggaa
     7261 cacgctttgt ccgaacaggg gaaaagcctg ctgatgtcgg gcattgccgg aatcgaaggg
     7321 catttttccc gtatggacac cgtaaccgtg tacagcaagg caaccaaaca gcccctgggc
     7381 aaagggcgcg tcctgttcgg ttctgccgcc gccgaagacc tgctcaaatc gcgtaaggcg
     7441 aaaggcgtgt tcatccatcg ggacgactgg atttccatca cgcccgaaat acgcctgctt
     7501 ctgaccgaat tttagaaaag gaaacccatg tcaaacacac aaaaacagct tgcccttgcc
     7561 aaagcggcaa aaaaatccgt caacacggcg gatacggaag aaaaaaaccg cgcgctgctt
     7621 gctatggcgg acagtctgga agcggcggcg gcagatattt tggcggcaaa ccgtcaagac
     7681 ttggaagccg cggcaggcaa cattcccgaa agcatgaccg accgcctttt gttggacggc
     7741 aaacgcattt gcgcgatggc ggacggcatt agggcggtta ccgcactgcc cgaccccgtg
     7801 ggcgaaatac tggaaacctc gactctgccc aacggcttgg aaatcgtcaa aaagcgtgtg
     7861 gcgatgggcg ttatcggcat tatttacgaa agccgcccga acgttacgtc cgatgcggcg
     7921 gctttggcac tgaaaagcgg cagcgcggtc gtactccgca gcggcaaaga tgcattccaa
     7981 tccgcacgcg ccatcgttgc cgccctgaaa acggggttgg cacaaacccg catcgacccc
     8041 gacgcgctcc agttgattga agacaccggg cgcgaaagca gttacgaaat gatgagggcg
     8101 aaagattatc tagacctgct gattccgcgc ggcggggcgg ggctgatacg ggcggtggtt
     8161 gaaaatgccg tcgtgccggt cattgaaacg ggaacgggca tcgtccacat ttatatcgac
     8221 aaggacgcgg attgggacaa ggcgctccgt atcgtgtaca acgccaaaac cagccgcccg
     8281 tccgtgtgca actcgatgga agtgctgctg gtgcatgaag acattgctgc cgacttcctg
     8341 cccaagctcg aacggctgtt ggttcgcgac cgcatagaag ccggactgcc gcccgtccgc
     8401 ttccgtttgg atccgcaggc ggcgcggcat atcggcggcg aagcggcggg tgcagacgat
     8461 ttcgataccg agtttttaga ctacatcctc gccgtgaaaa ccgtcgcttc ggtcgaagag
     8521 gcggtcgggc acatcgaaac ccacggcacg caccattcag acggcatcgt taccgaaaac
     8581 cgccacgctg cggactattt cacgacccat atcgattctg ccgccgtgta tgtcaacgcg
     8641 tccacgcgct ttaccgacgg cggcgaattc ggcttgggtt gcgaaatggg catctccacg
     8701 caaaaactcc acgcccgggg cccgatgggt ttgaaagagc tgacgagtta caaatacatc
     8761 gtacaaggca cgggacaggt tagggaataa tccgaattca aacgtctgat atagtggatt
     8821 aactttaaac cggtacggca ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg
     8881 ctgaagcacc aagtgaatcg gttccctact atctgtactg tctgcggctt cgtcgccttg
     8941 tcctgatttt tgttaatcca ctataaaaat gccgtctgaa acagtcttca gacggcattt
     9001 ccatttgcaa aaaatcaagc attgtccaag gggacgagaa tcgtacggtt gccgttgtgt
     9061 tccggtgcgg acacaatgcc ttccgcctcc atctggtcaa tcagccgcgc ggcgcgattg
     9121 tagccgatac gcaaggcgcg ctgtacgccc gaaatactgg ctttgcgcgt tttcaggaca
     9181 acggatacgg cttcatcata catcggatcg gtttcgtcgt cgccgctgcg cccgatgccg
     9241 ggcagctctt cgctgccgcc gccgctcaaa atatcgtcaa catagtccgg ttcgccaaac
     9301 tgcttcagat attcgaccac gcggtgcacc tcttcatccg aggcaaacgc gccgtgaacg
     9361 cgctgcggat aggccgtacc cggcggcagg aacagcatat cgccctgacc gagcaggttt
     9421 tccgcgccca tttggtcgag aatcgtgcgg ctgtcgattt tgctggatac ttgaaacgcg
     9481 atacgcgtcg ggatgtttgc cttaatcaga cccgtgatga catcgacgct ggggcgttgc
     9541 gtggcaagaa tcaaatggat gcctgccgcg cgggcttttt gggcgaggcg ggcaatcagt
     9601 tcttcgattt tcttgcctgc cgtcatcatc aggtcggcaa actcatcgac cacgaccacg
     9661 ataaacggca gtttttccaa aggttcggga tcgtcgggcg tgaggctgaa cggattgccg
     9721 attttttctc cccttgctgc ggcttcggcg attttttgat tgaagcccgc aagattacgc
     9781 acgcccataa agctcatcag gcggtagcgt ttttccattt cgttaacaca ccagttcagc
     9841 gcgtttgccg ccagcttcat atcggtaacg acaggggcga gcaggtgcgg gatgccttcg
     9901 taaatgctca attccagcat tttcggatcg atcataatca tacgcacgtc ttccggcgcg
     9961 gctttgaaaa gcatagacag aatcatcgcg ttgacaccca ccgatttgcc cgaaccggtc
    10021 gtgccggcaa ccaacaaatg cggtgctttt cccaagtcgg ttacgacggg ctgtccggtg
    10081 atgtcctgac cgagcgcgag cgtcagcttg gatttggatt cggcaaactc gggcgaattg
    10141 aagatttcgc tcaggcgtat catttggcgt ttcgggttcg gaagttccaa acccatgcag
    10201 gttttgccgg ggatggtttc gacaacgcgg atggaagcca cgccgagcga acgcgccaaa
    10261 tctttttcca gattcagaac ggaattgccg cgcacgccga catcgggttc gatttcataa
    10321 cgcgtaatta cggggccgga ataagaatcg acaaccttga ccttgacttt gaactccgcc
    10381 aatttttctt cgatggtgat gctgttttcc aacagttctt cttcggtttg cgtcgcctcg
    10441 ggattgaaca gcggaggcag aagcaggtcg gttgtcggca ggtgttcgga taccgcaccg
    10501 gtttcttcag atgggaacgc cccttcatcc gcttccgtat ccgatacccg gcaggacggg
    10561 cgttcagacg gcacatcttc aaacggacaa atcgcctgac tgtcatgccc gaacgcttcg
    10621 gtttccgaca gatattgccc gtttgaccgc tctgccgcac cttcactgcc gtcatctgca
    10681 atagcggcgg tttcctcctg ccaacctcca ttcaaaacat catcggcaag atggtcggtt
    10741 tcagcaatgc ggctgcgttg cgcctgctcg aatcctgtcg gcggctcata ggtacggttg
    10801 tagatttccg atacgggagg cggcggcgga atatcgattg cgggcatggg aactttcggc
    10861 acttcgggtg gttcgaccac ccaagcatcg ggcgcagcag gttcttcaat atagatatcg
    10921 gcagcttcat cataaaccgg accgccgtat cccacagacg aaacggtttc cgtgaaaaca
    10981 ttttcggttt cagacggcat ttcgacatcc cgtttcccga caaccgtccg actttccgga
    11041 atcaggcgcg aaatccgcgc ctccgcagaa accgttgcct tgttcggttc tttcaaattg
    11101 acggaaacgc ggcggcaggc atcgcggacg gctgtgccgt ctgaaacgga ctgtccttgg
    11161 gaaacatccg gggattttgc ctccgcctgc cctgcatgac ggcggaacgg cgcattccgt
    11221 gcattttcgg ctttgactgc agaaaaggca gacggatgct gcttctcaaa atacggctca
    11281 aatccgtaat ccgcagactc ggaaaacgct tctttgtccg catcgaacat acgggaatac
    11341 gtgcgttgaa gcacagggtc gtccaaaccg ataatctgca gcccttccat cggggtatcg
    11401 gacacgcgga ctttggggac cgctgtttcg tttttctcaa atgcatcgat atagcgtttt
    11461 ttcgtttccc ttaaagccgc gccgtttaag gcacgcgttg cttcttccaa agtgatttcc
    11521 ttaaaaaccg gacggacggg cgaaatttcg ctttcagacg gcatcaaccc ttcagcaata
    11581 ctccggtcga atgggatgcg gcggttgtcg attacggcag ttgcaatgtc ttccgtatcg
    11641 gcagcctctt cctccgcagc ttctgctgct tccgtttccg cttcttccgt cccgtttcca
    11701 ctgtctgaat atccgtcgga tgcgttcgga acatccgcac ggtttgcaga agcaggcgga
    11761 acaggcctat ccggttcgga ttcgtgcgcc agatagtttc ggcagaaacg gacgacaccg
    11821 acgaacagcc aatacactgc cgttttgacg gcatggaaaa gcatcagggc aagttcggga
    11881 aaatcgggca taccgtcttt gatttcaggc agctccgcct gtttttcccc tttcttttcc
    11941 tgccacgcag aaacctcgcg catccactcg cgttcggatt gtgcgcggac aaaaaacaag
    12001 ccggcaagcg caagcaacaa aataacgatc ataactatcc aaaacatatg ccgtttcccg
    12061 caagacaaac gattaaacgg gtttatttta acgtgtttcc atcccaaaaa aaacaaccgg
    12121 ccggcaattc tgccgaaacc gcttcggctt tcggtttacc ccaccgacag tataatagcc
    12181 gtcctttccc ttaatctttg cggcattgcc atgattttcc aaacagtctg gttttcagat
    12241 gtggtactgt cggtttcgtg gattgtcctt atcctgatac tggcagcttc cgctccgtcg
    12301 gcattccgct cgcttgcccg ctaccgttct gcccttcccc tgtgtaccgt aattttctcc
    12361 gcggcatggt gtctgaacgc ctctgccggc ggcggacagc ttgcccagat gaactaccac
    12421 ctgctcgccg tcaatctggt tgcactgatg gtgggcactt ccgccgcctt ttggcttgcc
    12481 gcgctgctga tgctgtccta ctgcctgctg tttgccggtt ctgccggcgc atatccgccg
    12541 aacgctttag tgctgattct gcccgcgctt gccgtcaacc gcctgtcgcg tatgctggtc
    12601 aaccgcctgc cgcccaatat tttcatcttt attttcgtca acggctttct cgcttccgcc
    12661 gccggcattc tgttgaccgg gctggtgctg accggtattt tggatgctgc aaacgctttt
    12721 ccgtccgaaa tattgtggac gaccgccctg cccgtcttta ttctgctggc gtgggcggaa
    12781 gccttcctca gcggcatttc aactgccata tttgttgcac tgaagcccca ttggattaac
    12841 actttcgacg acaaccgcta tcttaagtcc gaccgcggca tatggcggta gccttcagac
    12901 ggcatagaca ggcaaaacca tgctttccaa catcatcccc ctttccatcg gtgcggcact
    12961 cggtgcaacg gcgcgttggc tgctcaacct cgccgttccc gcatcgttgt ctcccgccac
    13021 gggcaacctg tttgccaact ggacgggcgc gctgctcatc ggcatctttg ccgaaaccgt
    13081 caaccatccg caatggaagc tgctgctgat taccggtttt ctcggcagcc tgaccacgct
    13141 ttccggattt tcactggaaa ccgtaaccct gctccaatcg aatcgtcctg cttcagcact
    13201 tgccaatatt ttcctgcaca cggcaggttc gctgctgctg acttggctcg ggctgaagat
    13261 agggacggca gtcaaataat caaaaaaatt gcttcctttg gttttgacac aaactataca
    13321 agtcctaaca t
//
LOCUS       DAWFJR010000050        12928 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWFJR010000050 DAWFJR010000000
VERSION     DAWFJR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12928
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.050"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            203..1564
                     /locus_tag="WGR04_001302"
     CDS             203..1564
                     /locus_tag="WGR04_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ0794849.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTIIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSSKLGFDLSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPVPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
     gene            1737..2060
                     /locus_tag="WGR04_001303"
     CDS             1737..2060
                     /locus_tag="WGR04_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794850.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(2171..3124)
                     /locus_tag="WGR04_001304"
     CDS             complement(2171..3124)
                     /locus_tag="WGR04_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ0794851.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHNVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(3263..4508)
                     /locus_tag="WGR04_001305"
                     /pseudo
     CDS             complement(3263..4508)
                     /locus_tag="WGR04_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            4654..5358
                     /locus_tag="WGR04_001306"
     CDS             4654..5358
                     /locus_tag="WGR04_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ0794852.1"
                     /translation="MLRSILAASLLAVSFPTAAEALNYNIVEFSESAGVEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNSFNRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQADAALEYTDFYVSRERRNEVIDQVSKDAVLRFK
                     ARADKLSGILGTSGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASYGVEGTDSAA
                     PGVEEISISVNGTVQF"
     gene            complement(5422..5988)
                     /locus_tag="WGR04_001307"
     CDS             complement(5422..5988)
                     /locus_tag="WGR04_001307"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ0794853.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(6057..6473)
                     /locus_tag="WGR04_001308"
     CDS             complement(6057..6473)
                     /locus_tag="WGR04_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794854.1"
                     /translation="MLKHLAFLLPAMMFALPTSAAVLTSYQEPGCTYDGDVGKDGKPA
                     GKGTWRCQDGRNYTGSFKNGKFDGQGVYTVASGREIFLEPFNSDSTKFRNMALSGTFK
                     QGLAHGRFAASQNGETLFIMKCEHGMIKEVKLPKNK"
     gene            complement(6525..7424)
                     /gene="rdgC"
                     /locus_tag="WGR04_001309"
     CDS             complement(6525..7424)
                     /gene="rdgC"
                     /locus_tag="WGR04_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ0794855.1"
                     /translation="MWFKQISFYPLNKEKLPEADALADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNIGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLIWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDATSLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            complement(7455..8912)
                     /gene="der"
                     /locus_tag="WGR04_001310"
     CDS             complement(7455..8912)
                     /gene="der"
                     /locus_tag="WGR04_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ0794856.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEKEEEEAKHPVFAVIGRPNVGKSTLVNAILGEERVITFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(9066..9695)
                     /locus_tag="WGR04_001311"
     CDS             complement(9066..9695)
                     /locus_tag="WGR04_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ0794857.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAILAEIVEKAQSKAPQSEINAELTKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(9696..10991)
                     /gene="hisS"
                     /locus_tag="WGR04_001312"
     CDS             complement(9696..10991)
                     /gene="hisS"
                     /locus_tag="WGR04_001312"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ0794858.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVGRWTRTYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVGEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTHTLQQWKNA"
     gene            complement(11085..11627)
                     /locus_tag="WGR04_001313"
     CDS             complement(11085..11627)
                     /locus_tag="WGR04_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0794859.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVL
                     KKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFT
                     HHPKRQTEFAVEQIRQARAE"
     gene            complement(11799..11990)
                     /locus_tag="WGR04_001314"
     CDS             complement(11799..11990)
                     /locus_tag="WGR04_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794860.1"
                     /translation="MLPTRSVSSLLLGEYLEFEENGTKIAAEIDSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
     gene            complement(12023..12559)
                     /locus_tag="WGR04_001315"
     CDS             complement(12023..12559)
                     /locus_tag="WGR04_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794861.1"
                     /translation="MEKIIDSKVRETNFNQAWRVIRAMQVDNISDEQVVIVRMSCYLE
                     KDEIFKNVERYGYQSSDIKKISPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKSGETLYSNFKSIKNQCKNYFFCGFDFDAPYFKSGV
                     SQVADYNFVPDAFSLFFI"
     gene            complement(12727..12927)
                     /locus_tag="WGR04_001316"
     CDS             complement(12727..12927)
                     /locus_tag="WGR04_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794862.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
BASE COUNT         3033 a         3108 c         3391 g         3396 t
ORIGIN      
        1 ccgccacaaa agcagggtct gattttttta aagggcaatc tgttataatg gcgcgttgcc
       61 gccgccgcga gggcggcgtg attcggacgg cgtagtttct acgccttttg tttatggtta
      121 cggcatcttg cggaatcgta ccgcctgatg ccgtctgaac acggttgccc gtggagatgc
      181 cgctcttcgg gttagaatat ttatgctgaa aaaatggttg aataagatgc tgccttccgg
      241 tcggagcagt aaaaaagcgg aaagtaaaac aatcattcct gccgaaagac acaacatccg
      301 tgccgaaatg ttgagctttg ccgccgaaaa cgtcatacgc cgcctgaaag gggcggggtt
      361 tcaggcttat gtggtcggcg gcgcggtcag ggacctgctg ctcggcatcg aacccaagga
      421 tttcgatgtc gcaaccgatg ccacgcccga acaggtgcac aaactcttcc gccgcagccg
      481 catcatcggc aggcgttttc agattgtcca tgtgatgaac ggtgcggaga ttatcgaagt
      541 aacgacgttt cgcggcggtg cgaaggttca tcagaacgcg cacggcagaa tcatgaagga
      601 caacacctac ggcagcatcg aagaagatgc gatgcggcgc gattttacct gcaatgcctt
      661 gtattacgat cctgaaaaag aagagatttt ggatttccac aacgggattg ccgatgttgc
      721 cgcccgcagg ctggttatga ttggtgatgc cgccgaacgc tatcaggaag accctgtcag
      781 gattttgcgc gccatccgcc tgtcgagcaa attgggcttt gatctgtcgg aagaaaccgc
      841 cgcaccgatt gccgaatcga tatgccgtct gaagcacgaa ccggtagcgc ggctgttcga
      901 cgaaattatg aaactgctgt tttcagggca cgcgcgcgag tgtctgaaac gtttgaacgg
      961 atttgacata ccggacgaca tccatccgct gctcaatgcc ttgcgcgttt cagacggcat
     1021 cgccggaaaa atgacggtgc ttgccctgaa aaataccgat gagcggctgc gtgccgacaa
     1081 atcggtttcg gtcggtttcg tactggcggc cctgatgtgg cccgagttgg aacgccattg
     1141 gaaaagcaat ctgcaacagg gtttgaaacc cgtgcccgcc ctgtccgatg caatcaatac
     1201 gatgcgcgaa accgtcgaac gcggttgggg cgtgccgcaa cgcttttccg ccacgatgcg
     1261 cgaaatttgg atgttccagc cgcagtttga aaatcgcaaa ggcgcacgtc cgcacaaact
     1321 gtttgcacag gcgcgtttcc gtgccgccta tgatttcctg ctcttgcgcg ccgaaaccgg
     1381 caatgcggac cgcgcccttg ccgagtggtg gacggcgttt cagacggcat cgacggaaca
     1441 gcggtcggag atgaccaaaa acgaagccgc cgcccgacac gaaaaaaacg aaggacaggc
     1501 gaaaaaacgc cgccgtcgca ggcgcaaacc caagccgaag gttgtgggaa cggattggga
     1561 ataagggtca acagacaagg agcaatgaag tttcaacaca tgggatgaag cataaagtgc
     1621 cgttctatgt attatcctga tttgtaaggg gattcatccc cgtaaataaa gtctaaccct
     1681 gcctctcgga aaaaggatgt ccgggtgggc agggttcaag aaacaaggaa aaattgatga
     1741 aaaaatgtat tttgggcatt ttgaccgcgt gtgccgccat gcctgcattt gccgacagaa
     1801 tcggcgattt ggaagcacgt ctggcgcagt tggaacaccg tgtcgccgta ttggaaagcg
     1861 gcagcaatac cgtcaaaatc gaccttttcg gttcaaattc caccatgtat gtatgcagcg
     1921 ttacgccttt tcagaagacg tttgaggcaa gcgatcggaa tgaaggcgtg gcgcggcaga
     1981 aagtgcgtca ggcgtgcaac cgcgaaactt cggcaatgtt ttgcgaagat gaggcaatcc
     2041 gatgcagaaa attcgattga tgtatcggtt ggacggataa agaaacggat acggatacgg
     2101 agcttggctt ccgtatctgt ttttctctgc ccgattttcc atgcatcggg tttcagacgg
     2161 cattggaatg tcagtcgtgt tctgccgatt cgtaggcttc gacgattttt tgcaccagag
     2221 gatgccggac aacgtcttcg ccggtaaagg tgtggaaata cagcccttcc acgttgtgca
     2281 gtttctcacg cgcatctttt aatcccgatt tgatgttttt gggcaggtcg atttggctgg
     2341 tgtcgccggt aatgacggct ttcgcgccga agccgatgcg ggtcaggaac attttcattt
     2401 gttcgggcgt ggtgttttgc gcttcgtcga ggatgatgta tgcgccgttg agcgtcctgc
     2461 cgcgcatata ggcgagcggg gcgatttcaa tcaggccttt ttcaatcagc ttggttacac
     2521 ggtcaaagcc catcaggtca tagagggcat cataaagcgg acgaaggtag ggatcgactt
     2581 tctgggtcag gtcaccgggc agaaaaccca gtttctcgcc ggcttcgacg gcaggccgca
     2641 ctaaaatgat gcgttcgact tggtgttttt ccatcgcatc gacggcggcg gcgacggcga
     2701 gataggtttt gcccgtacct gccggcccga gaccgaatac gatgtcgtgg ttgagtaggg
     2761 cgcggatata gccgttctgc cgtggcgttc tgccgccgat gctgccgcgc ttggtgcgga
     2821 aataataggc gtggtcatgg tttttttctt gatgaccggc atcttcggtt tgggcttcga
     2881 cggcggcaag cctgatgtcg ccgtcgttta ggtcgcgcgt ctgcgccgtt tccaagagtt
     2941 tgagcagtgc gcgtttgccg gcgtgtgcaa atgcgccgtt gaaagtgaaa tgttcaaaac
     3001 ggcggctgat gtggatatcg agtgctttag caagtaaatc aaggttgttg tcaaaagaac
     3061 cgcacagacg ctgcaacgcc aagttgtcgg tttcttctaa atgcaggtgg acggtatgtg
     3121 tcatatgaag gtccgaatag ttggatattg tgtgatttta atctatagtg gattagattt
     3181 aaaccagtac agcgttgcct cgccttagct caaagagaac gattctctaa tgtgctgaag
     3241 caccaagtga atcggttccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga
     3301 tttttgttaa tccactatat ttcaccggta ttttcttacc gtattctgcg attgcctgtc
     3361 ggaaaatgcc gatcaacctg cctataacgg cattttcgcc aaattcgttc agacagtttt
     3421 ccctaagtcg ggcaggttca aaatcagagt ggtgttcata cattttgatg agtgcgtcgg
     3481 caagggcatc gtcgtcgtca acaggaacca aatatccgtt gccgtctgaa acaatagatt
     3541 ccgcaccgcc gcagcgtgtt gcaatgacgg gcaatccttg ggacagtgct tcgatataga
     3601 ccacgccgaa ggtttcggtg cggctggcaa ggacgaatgc gtcgctgttc ctcatcaaat
     3661 ccaagactgc ttcgggctgc aatgcgccca aaaatgtaac ggcatgggta atgcccaagt
     3721 ctgccgcctg ctgtttcagc cgctgttctt cctgtccgct gccgccgatg tttagacgca
     3781 gttgcgggca ttgtgccaac gcccgggcaa aggcagtgag caggacatcg tgtcctttga
     3841 gacggcgaag gtgcgagacg gtgcagaaca cgaaatgcgg tttgttgttt ttttctcttt
     3901 gttcaaacgg tcggttgaat attccgccca gtatgttggg gaggtactgc cattcgcagc
     3961 cgtatttgtg ttgcaggacg tgtgcgaaat ggcggctgat ggcgagacgt gcggcggcgt
     4021 gcgccgccgc atttttcata ggctgccatt ggtggggacg gatcagaccg cgcgtgatgg
     4081 tgctgctgtg tttcgtgacg acatagggga tgccgtattt ttgggaaatc ttgaaggcaa
     4141 gtatgccggc atagttcata cagtgggcgt gaatcaggtc gggcagcccg ttttcgcgga
     4201 tgtagtgttt gaaagctttc aaacccgcac acacccagcg gatgcagtcg atgtcgatga
     4261 acggaaagcg ggggaagaaa tatatgccgt gccatgcata gatgtccaaa ccgctttgcc
     4321 gatattttgc aaaaccgtaa ggccggtcag gatgcttgct gtttctttcc gcaagtagcg
     4381 gaacatcggt gcaagcacgg cggttttgat gcctttcctc tgcaatgcca gtgcctgatt
     4441 ttgaaaaaaa tcccgtccac atcctgttcg gattgcggat accatgaggg gatgacgagg
     4501 acgtgcaacg gttcggacat aatatgattc cgtatcggaa agacggttat tataagacag
     4561 acgcagaccg aatatttaaa ttgttgcctt acgctaatgc aatttagcgc gccgatatgc
     4621 tagattggcg gttttatgag tgaggatacg gatatgttgc gttctatttt ggcggcttcc
     4681 ctattggcgg tatcttttcc gacggcggct gaagcattga attacaatat tgtcgaattt
     4741 tccgaatcgg cgggtgtcga ggtggctcag gatacaatgt ccgcgcgttt ccaggtggcg
     4801 gcagaaggac gggacaaaaa tgccgtcaat gctgagtttg ttaaaaaatt caacagtttt
     4861 aacagaaaat cgaaaaacgg cagctttaaa accgaattgg tatcgcgcag tgcgatgccg
     4921 cgctaccagt acaccaacgg cagacgcatt caaacaggtt gggaggagcg tgcggaattt
     4981 aaggtcgaag gtagggattt tgatgcttta aaccgtttta ttgccgatgt tcaggcagat
     5041 gccgcgttgg aatatacgga tttttatgtg tcgcgcgaac gccgaaacga ggtcatcgat
     5101 caggtcagca aggatgccgt tttgcgtttc aaggcgcgtg ccgacaagtt gtcgggcata
     5161 ttgggtacgt ccggttataa aatcgtcaaa ttaaatttgg gacacatcgg cagccatatc
     5221 gcgggtgggg gagctgctca ggcaaaaatg cttcgtgcca tgccgatggc ggcaagttac
     5281 ggcgtggagg gtacggattc cgccgcgccg ggtgtggagg aaatcagcat cagtgtcaat
     5341 ggaacggttc agttctaacc gcggatcaac aggtagatgc cgtctgaaac cggatgatac
     5401 ggttcagacg gcatttatat tttaggcttt aggcagggta acgccggttt gccccatata
     5461 tttgccgttg cggtctttgt aggaagtttc gcagatttcg tcgctctcga agaagaggac
     5521 ttgcgccacg ccttcgccgg cgtagatttt ggcgggcagg ggggtggtgt tggaaaactc
     5581 gagggtaacg tagccttccc attccggctc gaacggggta acgttgacga taatgccgca
     5641 gcgggcgtag gtggatttgc ccaggcagac ggtcaggacg ttgcgcggga tgcggaaata
     5701 ttcgaccgtg cgcgccagtg cgaaggaatt gggtgggatg atgcagcagt cgtcttcgac
     5761 ggtaacgaag tttttgggat cgaagttttt gggatcgacg atggtgctgt tgatgttggt
     5821 aaaaatttta aattcgtttg cgcagcggat atcgtagccg tagctggacg tgccgtagga
     5881 gatgatgcgt ttgccgtcgg cttctttgat ttggttcggc tcgaagggat caatcatgcc
     5941 gaattcttcg ctcattcggc gtatccattt gtcggacttg atgctcatag tgttttcctt
     6001 gtttcttgca gtgttcggac aaagcattgg gggatgccgt ttgaaagatg gggggcttat
     6061 ttgtttttgg gcagtttcac ttctttaatc atgccgtgtt cgcatttcat aatgaagagg
     6121 gtttcgccgt tttgcgaggc ggcgaacctg ccgtgcgcca agccttgttt gaacgtgcca
     6181 gacaatgcca tattgcggaa tttggtactg tcggaattga acggctcgag aaatatttcg
     6241 cggccggagg caacggtata aacgccttgt ccgtcgaact tgccgttttt aaacgaaccg
     6301 gtatagttgc gcccgtcttg gcagcgccat gtgcctttgc cggcgggttt accgtctttg
     6361 ccgacatcgc cgtcgtaggt gcagcctggt tcttgatagg aagtcaggac ggcggccgaa
     6421 gtggggaggg cgaacatcat ggcgggcagt aggaatgcga gatgtttaag cataagggtt
     6481 attccattgg attttggctg acggtataaa agctgtctga aaaatcaatc ttgccagccg
     6541 cctaaatagg aaaccagctc ttccaacatg gtgctgacgg attccgccat caggatttgc
     6601 gatgcgaagg caaggctggt ggcatcgtcg ccgttgcttt cggcttcttc ctgcaatacg
     6661 tcgaggtatt ggatgcgctt gagtgtaaag tcttgagtga ggataaaggc gatttgttcg
     6721 cgccaaatca aaccgagctg ggttacggtt ttaccgtttt tgacgtgttg aaccacttcg
     6781 tcggcggtta aatcttgttt ggatactttg acgacgggaa caatatcgcc cgtacctttg
     6841 agttcgcaat cgctgtctaa ttcaaaaccg ccttcgcaat gcccttgcaa cagccagccg
     6901 gtcatcaggg aagagggcga ttgtttggta ttcggcagcg aggcttccaa accgcccaaa
     6961 gcttcgcgca gcttggtcag gatgttttct gctttggcgg aagccgcgtt attgacgagc
     7021 aggtagccgt ggcgggtgtt aaacaccgct tctgtacggc tgctgcgggt aaacgctcgg
     7081 ggcagcaggt cgtcggtaat ttgctcttta agctcttgtt tttctttgcg accgatattg
     7141 cgggcttcat tgttttggat ttccgctacc ttctcttcca aaatatcgcg gatgacgccg
     7201 gcaggcagga ctttttcctc ttttttcagg gcgacgcgca aggtaaagtc ggcagggaaa
     7261 acgagttccg gggagaatga aaccggtgcg gtaaagcctt cgctgaacca gtctaagcct
     7321 tggcaatggg taaattcagc ttcagcaagt ttatcggcaa gtgcgtccgc ctcaggcagc
     7381 ttttctttgt tgagcggata aaaactaatc tgcttgaacc acataatgtt tcctattgtt
     7441 tgaaatgtcg ggaattattt gctgaattgt tttttcacgc tgactttggt tttcttcttg
     7501 aagcggtttt tctcttcctt tcggccttcg cgtttctcaa tacggttgct caggctgacg
     7561 cggcgcagcg gttttttctt cggtttgtct tccgcatttt catacgggtt ttccgaaaca
     7621 ttgtattgaa ttctgagcgg cgtgccttgc agattgaagg ctttgcggaa cgtttgggtc
     7681 agatagcgcg tatagctgtc ggaaatcgcg tgcagcgaat tgccgtgtac cacaattacg
     7741 ggagggttca tgccgccttg gtgggcataa cgcattttcg gacgcaccaa gccggcacgc
     7801 ggcggttgct gacgctcgat cgcgctttgt aatacgcgcg tgattttcgg cgtcggcatc
     7861 ttaatcatcg ccgcgttgta ggcagcctga atgctgtcaa acaaaccgtc tataccgcgc
     7921 tctttcaatg cggaaataaa gtggaacttg gcaaaatcga ggaaatacag tttgcggttg
     7981 atatcgcgtt tcacttgctc gcgacgttct tcgctgatgc cgtcccattt attgaccgcc
     8041 accaccaaag cacggcctgc ctccaaagcg aaacctgcaa tcgtcgcatc ttggtcggcg
     8101 atgtcctgct gcgcgtccaa taccaaaaca gcgacgtttg ccgcttcaac cgcctgcatc
     8161 gctttgataa cggagaattt ttccactgct tcatccactt tgccgcgacg gcgcacacct
     8221 gcggtatcga tgatggtaaa cggtttgcct tcgcgctcga aatcgatatg gatactgtcg
     8281 cgcgtcgtac ctgccatatc gaaggtgatg acgcgctctt cgccgagaat ggcgtttacc
     8341 agcgtggatt tgccgacgtt cggacggccg atgacggcaa aaacgggatg tttcgcctct
     8401 tcctcttctt tttcaggctc agggaaggtt tccaaaatat cttcaatcag ataatacacg
     8461 ccgtcaccgt gcgcacctga aataacataa gggtcgccca aagcaagttc gtagaactcg
     8521 gcggcaagca cggctctatt gccgccttct cctttattca cggccaaata aacggggcgc
     8581 gggctttggc gcaaacggtc ggcaatgatt ttatcttgcg gcgtcagacc ggtgcggccg
     8641 tccaccaaaa atacgacggc atcggcttca tcgacagcct gcaaggtttg ttttgccatt
     8701 tcgtgcaaaa tgccgctgtc cacaaccggc tcgaaacctc cggtatcgat gaccaaataa
     8761 ggtttgctgc cgactttgcc gtgtccgtaa tggcggtcgc gggtcaggcc gggcaggtcg
     8821 tgtacgagtg cgtctttggt gcgcgtcaaa cggttgaata aagtagattt gccgacgttg
     8881 gggcggccga caagcgcgat ggtgggtttc attattgggt ctttctgtgt caagtgccgt
     8941 tcgggagaac tgaacacgag caggtgtccg ttggacacgg taaataggtt cttcacggca
     9001 agaggccata aagagggagg gtaaaatgcc gtctgataaa taatggacgg catttcagac
     9061 ggcatttatt tcagcgaatc aagtttcatt tgaaccaatt cgcgaccgac agaatcttga
     9121 ggcatttttt ctaaagcctg tccgtagttt tttaaggctt cctggctttt tccctgtgcg
     9181 gcatagacat cgcctttggt ttccatcagc aggggggcga agtccgcttc aaccggcgtg
     9241 tcgagcgcgg caagtgcggc atcgtatttt ttttgttgca acaacacaac gcccagacgc
     9301 tgcgccgcca acgcttgaat caggctgtct ttttggttgg acaacaccca tttcaaatgg
     9361 ccttcggcaa catcgtaacg ctgtgcgtca aattcggttg ccgccgccat cagcgtggct
     9421 tgggcggcgg aaatggaatg cgggtagctt tgttggagtt tggtcaattc ggcattgatt
     9481 tcgctttgcg gggctttgct ttgcgccttt tctacgattt ctgccaatat ggcagcggct
     9541 tcctgatttt gggaagccgc acggttttgg taaaccgtgt atcccaagta gccgagtgct
     9601 gccaaaatca gcagggcaaa cagccatttg cccgtggttt tccaaaaata tttaaaattg
     9661 tctaactctt gttgttcttc gagatgggct gccatttatg cgttcttcca ttgttgtaaa
     9721 gtatgggtta aatcctcggc ggcgacagtt tgctgaccgt gtgcgccgtt catgtctttg
     9781 agcgtaaccg taccgttcgc cagttcgtct tgcgccacaa tcagggcaaa gcgcgcgccg
     9841 ctgttgtcgg cttttttcat ttgtgctttc aggctttgat agccggaatg ctgcattaca
     9901 ttgaaacctt gcgcgcgcaa ggcttgcgcg tatttcatca cctgcaagtc cgccccttcg
     9961 ccttggtgca ttgcatagac atcaggcgcg gcgtttactt ccagcgagcc gtattcgccc
    10021 accaaaagca gcagccgttc gatgcccatt gcaaagccga tggacggcgc aggcttaccg
    10081 ccaagttcct caatcaaacc atcgtaacgg ccgccgccgc acacagtcgc ctgcgcgccg
    10141 agtttgtcgg tcgtccactc aaaaaccgtc tgattgtaat aatccaaacc gcgaaccaag
    10201 cgcggatttt caatatattg aatacccaaa ccatccaaca tcgccttgaa gcgtgcatag
    10261 tggttttgcg aagcctcgcc caagtaatcc accaaacgcg gcgcggcatt gcagatttcc
    10321 tgcaaatcag ggtttttcgt atctaaaacg cgcaaaggat tggttttcag acggcgtttg
    10381 ctgtcttcat ccaatttatc ttcataacgg gtcagatatt caaccaatgc cgcacggtgt
    10441 gccgcgcgtt cctcacggtt gcccaagctg ttgatttcca aagtcaggta ttcgcgaata
    10501 cccaattttt cccacaagtc ggcagacatc gcgatgattt ccgcatcaat atccggacct
    10561 tcaaaaccca aagcttcgat accgacctga tggaactgac gataacggcc tttttgcgga
    10621 cgctcgcggc ggaacatcgg ccccatatac cacagctttt gcgggctgtt gtagagaagg
    10681 ttgtgttcga ccaccgcacg caggcaggaa gccgtacctt caggacgcaa gctcaaactc
    10741 aaagaatcgt ttgaatcgga gaaggtgtac atttccttgc cgaccacatc ggtttcctcg
    10801 ccgatggagc ggacaaacaa accggtttgc tcgacaatcg gcgtacggat ttgctgataa
    10861 ccgtaagtgc gtgtccagcg gccgaccgta tcttcaaacg cctgccaaaa cgcagccgtc
    10921 agtttgaaat ctttttgctc gacaggcaga aggtcgttca tgcctttgac ggattggatt
    10981 ttttgtgcca tttcaagtaa gaatgcttaa atcaaattgc gggcgattat agcggatttt
    11041 taagggtttg tgaggttggg ggtggtttgc ggacggcatt tgacttactc tgcacgtgct
    11101 tgcctgattt gttcgactgc aaactccgtc tgccgcttgg gatgatgtgt gaaaaacaat
    11161 ttatttgaaa ttgtctcggc ttttttcggc acgaccgcca aaatcttacc tgccaaattt
    11221 ccctcacggg tttgccaagc atccaaaaac tgcgccctgc tcatcgaaac atgccccagc
    11281 gacgggtcgg caagcaaaac cgtattgccg tctatcccgc gcaataccga gaaatgatca
    11341 tccttgcggt atttcagata cacgatgacg gggattttca actgcgcgag ctgctcgaaa
    11401 gacagggcat agcctttcgc ttcaaaaccc aaatcaggca taatgcgccg catatcctca
    11461 aacgacgcgc gcatctgctc cttatccagc tttttcaaca cttcttcttc cgtcagcgtt
    11521 tgcccgtaaa aattgttcaa aagcgtcgcc accgaagccg ccccgcagga aaaatccaaa
    11581 tcctgcttta caatattgaa atcgcgcctt tctttccaac tctgcacttt gatttttccg
    11641 taaacaacag gattatcgtt aaacatcggt gcagcattca aacgataaga taaagaaacg
    11701 acaacacacg ccaacagaaa aacatatttg aacttcatca tattttccac ataaagagca
    11761 gcctgaaaat ctttcaggct gcccttgtca aattattcct agctttcggc ttttttggca
    11821 aaccaaacaa tccgattacc cgcataatac tttccattta ttgaaatccg acaagccgcg
    11881 cccaaaaaat gccatgcact gtcgatttcc gcagcaatct ttgtaccgtt ttcttcaaat
    11941 tccaaatatt cacccaataa taaacttgaa acagaacgcg tgggcagcaa gtgcccaccc
    12001 tacgcttatt caaataattt gattaaataa agaataaaga aaatgcatca ggaacaaaat
    12061 tataatctgc cacctgactc acaccgcttt taaagtaagg ggcatcaaaa tcaaaaccgc
    12121 aaaaaaaata atttttgcat tgatttttaa tagatttaaa attcgaatat agtgtttctc
    12181 cagatttaaa aagtttatct ttatctatat atttgatgct ttccctatcc aaaataaata
    12241 tttcaaacat taaaaaatca ttacatgacc aagccaaatc ataaatttga ttcaaatggg
    12301 tatcataaag ataaaaataa taaggtttgg gaacaggtaa aatatttaat ggaaaaggag
    12361 gactaatttt cttaatatct gatgactgat atccatatct ttctacattc ttaaaaattt
    12421 catctttctc aagataacaa gacattctga caatcacaac ctgttcatcg gatatattat
    12481 ctacttgcat ggcgcgtata acacgccatg cctgattaaa attagtctcc cttaccttag
    12541 aatctattat cttttccaaa gatgaaaatg ccccttatct tgaactctcc agttagaacc
    12601 tagaagtttc gtacctctta aatgtgtatt aatatttctt atagtttcat taaaatgcca
    12661 cgcgctgccg atttcaacgg taattttcgt accgctttct tcaaattcca ggtattcccc
    12721 cattagctaa cgcaaagaag cagacgccat ttcggcttcg ttatgataaa cccgccttcc
    12781 gttgatatag acttccgccc ctgtccgctc caaattccaa aaattccgta ctgaaatttc
    12841 catatcccga tattgtgcag accatgtttt ttcgaaggtt ttcataaaat ttcctatacc
    12901 tgtccaatcg gcacatatca attgcatt
//
LOCUS       DAWFJR010000051        12886 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWFJR010000051 DAWFJR010000000
VERSION     DAWFJR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12886
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.051"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(53..361)
                     /locus_tag="WGR04_001317"
     CDS             complement(53..361)
                     /locus_tag="WGR04_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794863.1"
                     /translation="MTNNVDLKVPVKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKELLADLDKELC
                     "
     gene            complement(345..647)
                     /locus_tag="WGR04_001318"
     CDS             complement(345..647)
                     /locus_tag="WGR04_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ0794864.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(721..3084)
                     /locus_tag="WGR04_001319"
     CDS             complement(721..3084)
                     /locus_tag="WGR04_001319"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ0794865.1"
                     /translation="MQFSYSWLKTQADTELSADKLEHLLTMAGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLDRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     CVFEIARVFSKGSDGQFVQNERIGGLWYGAVMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRVVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3120..3638)
                     /locus_tag="WGR04_001320"
     CDS             complement(3120..3638)
                     /locus_tag="WGR04_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794866.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(3645..4709)
                     /locus_tag="WGR04_001321"
     CDS             complement(3645..4709)
                     /locus_tag="WGR04_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0794867.1"
                     /translation="MIKSFELEKFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="WGR04_001322"
     CDS             complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="WGR04_001322"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0794868.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEVKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDSEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            complement(6224..6583)
                     /gene="rplT"
                     /locus_tag="WGR04_001323"
     CDS             complement(6224..6583)
                     /gene="rplT"
                     /locus_tag="WGR04_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ0794869.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(6596..6793)
                     /gene="rpmI"
                     /locus_tag="WGR04_001324"
     CDS             complement(6596..6793)
                     /gene="rpmI"
                     /locus_tag="WGR04_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ0794870.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(6940..7461)
                     /gene="infC"
                     /locus_tag="WGR04_001325"
     CDS             complement(6940..7461)
                     /gene="infC"
                     /locus_tag="WGR04_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ0794871.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(7479..9392)
                     /gene="thrS"
                     /locus_tag="WGR04_001326"
     CDS             complement(7479..9392)
                     /gene="thrS"
                     /locus_tag="WGR04_001326"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ0794872.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKAAVAGRVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMSEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDRNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHSKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLNAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQLVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(9446..9522)
                     /locus_tag="WGR04_001327"
     tRNA            complement(9446..9522)
                     /locus_tag="WGR04_001327"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9486..9488),aa:Val,seq:gac)
     gene            9701..10816
                     /gene="tgt"
                     /locus_tag="WGR04_001328"
     CDS             9701..10816
                     /gene="tgt"
                     /locus_tag="WGR04_001328"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ0794873.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLKGLEELDFPGLAIGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGVDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMRDAIEQGKFADWQARFHENRARGTD"
     gene            10898..11908
                     /locus_tag="WGR04_001329"
     CDS             10898..11908
                     /locus_tag="WGR04_001329"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ0794874.1"
                     /translation="MLPFFPEPSLSYTQQNRTAVLLLNLGTPDAPTAQAVRPYLKSFL
                     TDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYEKIWFKEGSPLEVYTARQAAALAKR
                     MPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLVIPMYPQYAASSSGAAVDKVCEQLL
                     LQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAEHGRGKKLMLSFHGVPQKHYDLGDP
                     YPDECRHTAKLLAEALELTEDQYVVSFQSQFGRAKWVTPSTQDLFGKLPKQGVTELDV
                     FCPGFLADCLETMEEIALMGREQFYEAGGKSYRYIPCLNDNPDWIDALVALAEENLGG
                     WR"
     gene            12099..12567
                     /locus_tag="WGR04_001330"
                     /pseudo
     CDS             12099..12567
                     /locus_tag="WGR04_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692723.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
     gene            complement(12564..12854)
                     /locus_tag="WGR04_001331"
     CDS             complement(12564..12854)
                     /locus_tag="WGR04_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794875.1"
                     /translation="MSKYFTETDIGVREHFDFFGEFVVSPAARSDDLALAYGLKLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGDGNIGSMGLKVPR"
BASE COUNT         3277 a         3392 c         2857 g         3360 t
ORIGIN      
        1 attttttagc gtgtaaaaca aaaccggctg catacctgca accggcctca aatcagcaca
       61 attccttatc caaatccgcc aacagctctt tcagaccgtc tccgatttcc tgaagcgtaa
      121 ccggacggtt gccgtccgaa cccgactccg caccttctgc atacggtgca aacgtgctct
      181 gcaatttcaa cagtttcacc acatccaaac gggtgtagcg ttcgccgccg taaccgacaa
      241 ccacaccgtg atcatgctgc cattgcgcaa aaccataggg gctgatttgc aacagtccgc
      301 acaactcgtc cagcgtgaaa tagcgtttta cgggaacctt caaatcaacg ttgtttgtca
      361 tagtagtgtt ccaccatgct tttgagtttc tggctggcat ggaaagttac cacgcggcgg
      421 gcggtaatcg gcacttcctc gccggtttta ggattacgac ccgggcgttg cggcttgtcg
      481 cgcaactgga aatttccgaa accggaaatt ttgatttctt cgccgcttgc caaagtgctg
      541 cggatttctt caaaaaagag ttcgacgatt tctttggcat cgtttttggt gacgttgctg
      601 actttgtcta ccaaaatatc ggccagttct gctttagtga gagtcatatg attaccttct
      661 gttgtaacag ttgtggatta ttaccaattt ttatttaaaa ttcaagcgaa tatttatttt
      721 ttaactgcga agccgcgccc ctgccgccgt tgccgcgcca atcagttttc cgataagcgg
      781 ctcgactgcc tcatccgtca gcgtgttttc catatcctgc aaaatcactt tgaccgccac
      841 gctcttcatc ccttcgggca gtcccgtgcc gcgatacacg tcaaacacgc tgatttcctg
      901 taccaacttg tttgccgcgc ctttcaagac aagcagcaaa tcatcatggc tcatagcttc
      961 cggcatcaca aacgccaaat cgcggcgcac cggctggaat ttcgatacga cccgatagcg
     1021 cgttttcccg cattccaaca cggccgccat atcgatttca aataccagcg gcgcttgcgg
     1081 caggtcgtat ttttgcagcc atttcggatg cagttcgccg acaaagccga tgactttgcc
     1141 gtctgaaacg atattggcgg cacgtccggg atgcagggcg ggatgtccgg ttttaacgaa
     1201 ctcgactgct ttgtttttca acagattttc cacgtccgcc ttgatgtcgt aaaaatccgc
     1261 attgcgcgtt ttcccgcccc attgttccgg catgaccgcg ccgtaccaca atccgccgat
     1321 gcgttcgttt tggacaaact ggccgtctga acctttgctg aacacgcggg cgatttcaaa
     1381 cacgcacacg cggttttgtt tgcggttcag attgttttgc agaatttcca ccaagccgcc
     1441 gatgagcgtg gaacgcatca cggcatactg cgccgccagc gggttttgca ggcggatggg
     1501 gtcggcgttg gcggcaaaat cttgttccca ctgctcgtca acgaaggcat agctgaccac
     1561 ttcgcggtaa ccgcgagccg ccatttcgtt gtaaacggca aaacgcgggc ggcgtgtttc
     1621 gggcagttcc agcattttca gacggcctga cgtgtaatcg tcggggatgt tttcatagcc
     1681 gtaaacgcgt ccgatttctt caatcaaatc agcctcaatt tcgatgtcaa aacggaagct
     1741 cggcgcggta acgcggaagc cttccgccgt tttctcgggc tgcaggccca agtgttgcaa
     1801 aatggtttcc acctgttcgg caggaatgtc cacgcccaac acggttttca gacggtccaa
     1861 acgcaatcca acctgcttcg cttcaggcaa ttcgccttgc gcttccacca tctcgcctgc
     1921 cgcaccaccg caaatctgca acaccaattc ggtagcacgt tcaatggcat ccgcctgcaa
     1981 acggtaatcc acgccgcgct cgaagcggaa cgacgaatcc gaaccgaaac cgtattggcg
     2041 cgatttgccg gcgatgattt cgggcgcaaa ccaagccgct tccagcacga tattttgcgt
     2101 gccgtctgaa accgcgctcg ccgcgccgcc cattaagccc gccaaactca acacgccttt
     2161 ttcgtccgcc acgaccagcg tgttttcaga cagggaaacg gttttctcgt tcaggcattc
     2221 cagcgtttcc ccttcgcgcg cgcggcggat gtgcaggctg ccggaaagtt tgtcggcatc
     2281 aaaaacgtgc atcggctgac cgatttccag catcacataa ttgccgatgt ccaccagcgc
     2341 ggaaatactg cggatgccgc tgcgctccaa acgttgtttc atccaatcgg gagtagccgc
     2401 tttcgcattg acattttcaa taacgcggct gataaaacgg ccgcaatcgg caggcgcgtt
     2461 aatctgcacg ggctgttttc gactgcccgt gatcggcgcg gtatggattt cgggctgcct
     2521 gaacgcgcac cccgtcaatg cggacacttc gcgcgcaatg cctttgatgc tcaagcagtc
     2581 ggcgcggtta ggcgtaattt tcaacgtaaa cagcgtatcg tccaaatcca agtattcgcg
     2641 gatattggta ccgacgggcg catcttcagg cagaatgtgc aggccgttca caccgtcgtc
     2701 gggcagaccg agttcgtcgg tggaacacaa catcccgtcc gacaccacgc cgcgcatttt
     2761 ggtcggcttg attttgaaat tacccggcaa aacggcaccc ggcagcgaac acggcacttt
     2821 gatgcccgct ttcacattcg gcgcaccgca cacaatctgc accaacccgc ccgtacccgc
     2881 atcaactcgg gtaacgttca aacggtctgc atccggatgt ttttcaacgg atttcacttc
     2941 ggcaatcacc acgcccgcaa acgcaggcgc ggcagtttcg gcctcttcca cttccaagcc
     3001 ggccatggtc aaaagatgtt ccagtttatc ggcagaaagt tcggtatcgg cttgggtttt
     3061 cagccatgag taggagaatt gcatgttatg ttctcaatta actaattaac tttagatttt
     3121 caagagctag ctaaaccttt ttgaatggct aagtccctat taattacttc tacagaaatg
     3181 ttccataaag aaatcaaccg caaagtatct ttcttattaa attcttgctt tattaattca
     3241 ttcaattgcc caagataatt aagcggcaca tccgggataa ccagtaataa tttgatttct
     3301 atgctacccc attgcaaatt tgaaaaagca ctaggcataa ttgtttcttt gatatcttct
     3361 ctgcctaata ttcccaaaat ttcaacattt aatgcattac accatttttg ataaatctct
     3421 gaaatatatt catcaaaacg ctcaggagat ctttcttgat ttggaatact ttgttttgcc
     3481 tcaacaaata acaaattatc ttcttgcaga tagataaact ctactgtctt aataccttct
     3541 tgccttctct tccggatata tggttctata taaaacaggt tattttttac tagattaaaa
     3601 gttatacctg attcagtgag atttattatt tcactcataa aaacctaacc aatccatgct
     3661 gtttccggag ataaaacacc tatttcttct ctgaacaatc gaatagactc accaataatt
     3721 tcattatccg gcatgctatt ttgtaaattt tcctgcaccc aagtagtctg ttccttttct
     3781 ttgccaggca taaaaacagg aatactttca ctatgctttc tagctaataa acacagcttc
     3841 ttgataacaa aatatgaatg cgtactcata aaaatctgta ttcccaattt ggatttagat
     3901 aaggaaaaca gaatttctag aaattgatta attgctttcg gatgtagatt agcttctggc
     3961 tcatcaataa ataagattga tccaggactc aaaaaccgat ttcctaatag agtgtctaat
     4021 atcccaatct tttttacccc ctctgctgta gaattgatag agaaagtttg tctatctttt
     4081 tcatataccc atgccttctt tttatcattg tagctaacat aaccgccaaa caatgcttct
     4141 agatcctttc tagcttccgc tgcctcaggg taattcctcc cttttgttgg tttaatatcc
     4201 aatgctttta ccaaatcaat gtaagtagca tcaaaaccaa atctcttttc ttcctctgat
     4261 tttttaatta catcaaatag tgatagaact tctttgggtg gtaaaaaaat agagttagcg
     4321 tttattctgg gagaaagttc gtatagctcc ggtttaatta gctttttggt agttctacca
     4381 aaagaaaaca aaactgaaga actatcttct aaagtcatac ttaacttaag aggccgttcg
     4441 tttggatttt cttttcctct tgtaactaaa tcaccaattt cctctacttg gaacgtccaa
     4501 tatagcttat ctcgcaattc ctcagaaaaa tcacgaatat tctttccttt atgagcttcc
     4561 tcatgacttt ttaaaacact atataatgcc tttaaaataa aagttttccc tttgctattt
     4621 tctgcaataa ttaaatttat tttccctaaa ttatcagcct tcaataagct aatcggtccg
     4681 aatttttcaa gctcaaatga tttaatcata taatccccct aatatatatc aacaatattt
     4741 gtacaagcca cattgtaaaa agacagcaac cacagcacca tagtaaacgc atagcaggcg
     4801 gataatggag cgacatagcg tttcatgatt tagctttctg aggtaggcac aggctgcttt
     4861 tttcattttt aggcagccta cacaatcatt ttacaaactg cttcaaaaaa ttcaaatcgt
     4921 tatcaaaaaa caagcgcaaa tcgttcacgt tgtaacgcag catcgcgaaa cggtcgagac
     4981 cgataccgaa agcgaaaccg gtgtattttt cagaatcgat attcacattt ttcaacacgt
     5041 tgggatgcac catgccgcag ccgccgactt ccagccattt gccgttttcg cccataatgt
     5101 cgatttcggc agacggttcg gtgaacggga agaaagacgg gcggaaacgt acttgcaaat
     5161 catcgcgttc aaagaagcga cggataaaat ccgtgaacac tgcttttaag tcggcaaaag
     5221 ttacgccctc ttctacccac aaaccttccg cctgatggaa cataggcgag tgcgtggcat
     5281 cgctgtccac acggtaaacg cggccggggg cgataatgcg gatgggcggc tcttttttat
     5341 cgagcatata gcggatttga atcggggaag tgtgcgtacg caaaacatcg ccgttttcaa
     5401 cgtaaaacgt atcctgcatc gcacgggcag gatggtttgc agggatgttc agggcttgga
     5461 aattgtgaaa atcgtcttcg atttcaggcc cgtccgccac ttcgaaaccc attccgtgaa
     5521 agagttcgac cacacgttgc aaggtcaggg ttacgggatg caggctgccg ccttcctgag
     5581 cgcgtccggg cagggtaata tcgagggctt cggcggcaag tcgggcttgc agcttgactt
     5641 cgttgagggc atcgcgtttg gcattaaaag ccgtctgaaa ccggtttttg cattcattga
     5701 tatgcgcacc tatggttttg cgctcttcag gcgacatttg ccccaaagtt ttcagaagtc
     5761 cggtcaactc gccggttttg ccaagataac gggctttgat ttgttctaga gcgttgaagt
     5821 cttgcgcagc ttctactgcg gcaatgcctt ctgcaacgat gcggtttaca ttttccataa
     5881 tatcaagtct gtcaattaat gatatgccac ctgaaacaac gaggccgcct gtatagcctg
     5941 acatatcaga ctcttcagac gaccttattt tcttcacggt caatccattg gataaatttt
     6001 ctgtatagtg gattaaattt aaaccagtac ggcgttgcct cgccttgccg tactatctgt
     6061 actgtctgcg gcttcgtcgc cttgtcctga tttaaattta atccactata ttttaacgca
     6121 aatttattta aaaaaataga ctttaaagaa agaaaatcaa tcacatagaa tttctgcaaa
     6181 caaagaaaaa aggaagccgc agcttccttt tcaatttttt ggattaagca gccaaagcag
     6241 ctttggcttt ttcaaccaat tgtgcaaaag cggctttatc gaacacggcc aaatcagcca
     6301 atactttgcg gtcgatttcg atagaggcgc gtttcagacc attcataaat ttgctgtaag
     6361 acaacccgtt ttcacgcgca cctgcattga tacggacaat ccacaattga cggaattggc
     6421 gtttgcgttg gcggcggtcg cggtatgcgt attggccggc tttcattacc gcctgcttgg
     6481 caacgcggta aacgttttta cgacggccgc gatagccttt ggctaacgcg aagatttttt
     6541 ggtgacgggc acgagcggta acaccgcgtt ttacgcgtgg catattctaa actccttaag
     6601 cgtagggtaa cattttagca acagaagcca aatcgcgatc atttaccata gaggtaccgc
     6661 gcagttggcg tttgtttttg gtggtctttt tagtcaagat gtggcgtttg aacgcatgag
     6721 cgcgtttcac accgccgtta cccagtactt taaagcgttt tttcgcgcta gacttggttt
     6781 tcattttagg catgggaaaa ctccattcgt tatcggataa ggcattaggg ggttttaaaa
     6841 catcggtttc aaacacttga acccacaatg ccacggtttt ttgccgcaat tgaaaactta
     6901 ctccgaagcg gcaatcggag taagcggaga attatagctt tattttttct tcggcgcaat
     6961 catcatcacc atttggcgac cttccatttt gggaaaggac tcgatttgcg ccacttcagc
     7021 caaatcttct tttacacgtt ccaaaagttg cgcgccgagt tgctggtgag ccatttcacg
     7081 gccgcggaaa cgcaatgtca ctttgacttt atcgccgtcg gcaaggaagc ggttaatgtt
     7141 gcgcatcttg atttgataat cgccctcatc cgtacccgga cggaatttaa tttccttgat
     7201 ttgtacctgc ttttgatttt tcttggcttc gtcgcgcttc ttggcttgtt ggtatttgta
     7261 tttaccgtaa tccatcagtt tgcatacagg cggtttagca gttggggaaa tctctaccaa
     7321 atcgacatcc tgcccttcgg ccatagccaa agcttcacga actgaaacga caccaagctg
     7381 ttcgcctgac tcactgatta aacgcacttc tttggcggta atttcgccgt tgattcgtgc
     7441 ttcgcgttct tgagcgatga tgaatactcc tataaaaatt aatgattgac gagggcatca
     7501 gtgatttctt gctgcaattg cgcaatgaaa tcatccaaat ccaaagaacc caaatcttct
     7561 gctttgcggc gtaccgccac tttgttttcc tgcttctcct tatcgccgac aacgatttga
     7621 taagggaaac ggtattggct gttgtcgcgg attttgtaac cgattttttc gttacgcaaa
     7681 tccaactcgg cgcggaatcc tgccgcctgc aatttggcag ccacttcccg acaataatct
     7741 gcctgatttt cggtaatatt cataattacc aattgaaccg gagccaacca taacgggaat
     7801 gagcctgcat ggttctcaat cagaatgccg ataaaccgct ccaaagaacc taaaatggcg
     7861 cgatgcaaca taacaggacg cgcacggtcg ttgttttcag ttacatattc ggcattcaaa
     7921 cgctccggca atacgaagtc caattgcagg gtaccgcact gccaagagcg acccaaggca
     7981 tctttgacat gatactcaat cttaggacca tagaatgcac cttcgccagg caattcgccc
     8041 cattccacgc cgcaggcagt caatgcctcg cgcaaaccct gctctgcctt atcccacacg
     8101 tcatctgaac ctgcgcgttt ttcagggcga agagaaagct tgacggatac atcatggaaa
     8161 ccgaactgtt tgtagatgcg aaccaacaat tcattgaacg cacgggcctc gctgacgatt
     8221 tggtcttcgg tacagaaaat atgcgcatca tcctgcacaa atccacggac ccgcatcaga
     8281 ccatgcaatg caccgcttgg ttcattgcgg tggcaagaac cgaattccgc caaacgcatc
     8341 ggcaaatcgc gatatgagcg caaaccgtta ttaaaaattt gcacatgacc cggacagttc
     8401 atcggtttaa ccgcatattc gcgtttttcc gaactggtta cgaacatatt gtctttgtag
     8461 ttgtcccaat ggccggattt ttcccaaaag gttttatcca tgatttgagg cgttttgacc
     8521 tctttataac cggcggcgtt cagctcttta cgcatatgct gttcaatcac ttgccacaaa
     8581 gcccagcctt tagaatgcca aaacaccatg cccggcgctt cgtcttgcag gtggaacaaa
     8641 tccaattgct tgcccaattt gcggtggtcg cgcttttcgg cttcttcgat acgttgaata
     8701 taggctttca actcgtcttt tgtcgcccaa gccgtaccgt atatgcgttg cagcatttca
     8761 ttgttgcggt cgccgcgcca gtatgcgccc gccaacttgg tcagtttgaa gtttttcagg
     8821 aaacgggtat tcggaacgtg cggaccgcgg cacatatcga catattcctg atgatgatac
     8881 atccccatcg cttccacttc ggacatatcg tcaatcaggc gcagtttgta ttcttcgccg
     8941 cgctcttgaa aaattttaat cgcctccgca cgcggagtca tgattttgac cacatcatag
     9001 tcttgggcaa tcaattcttt catacgcgct tcaatggcgg caacatcttc cggtgtaaac
     9061 ggtttttccg tggcaatgtc gtaataaaag ccctcttcaa tgacggggcc gataaccatt
     9121 tttgcattag gatagagttg cttgacggca tgcccgacaa gatgggcgca ggaatggcgg
     9181 atgatttcga tgccttcctg atctttcgga gtaatgattt gaacagcaga atcttcaaca
     9241 atcgggtcgc acgcatcgac caatttgccg tttaccctgc ctgccaccgc cgccttcgcc
     9301 aaaccggcac cgatagacgc agcaatttga gccacggtaa cgggggattc gtattgtcgg
     9361 actgagccgt ccggcaaggt aatgttcaac atcaaaaagc tccaaaacat taaaaaataa
     9421 cggcataaat gccgttatgg gaatttggta ggcacgattg gattcgaacc aacgaccccc
     9481 accatgtcaa ggtggtgctc taaccaactg agctacgtgc cttcaaagaa tttttgcatt
     9541 ttatcgggtc gttttaattt ttgcaagagg tatgtgcgtt tctttatgca acattgcggc
     9601 aagatttggg gatgtcgtct gaaaaaccgt gggaaatcat ttataatgcc gtcttttaaa
     9661 acatatttcg ccccctgtcg cccaactgac attcaatctt atgcttaaat ttaccttaca
     9721 caaaaaagac ggatacgccc gacgcggcac gctggagttg aaccacggca aaatcgaaac
     9781 gccggtattt atgcctgtcg gtacttacgg ttcggttaag gcgatgaacc cgcaaaacct
     9841 gcacgacatc aaggcgcaaa ttattttggg caacacttat catttgtggc tgcgtccggg
     9901 cttggaggtc gtcgaacagt ttggagggct tcacggcttt atcggctggg acaaaccgat
     9961 tttgaccgac tcgggcggtt ttcaggtttt ttctttgtcg gatatgcgca agctgacgga
    10021 agaaggctgt acgtttaaaa gcccgattaa cggcgacaag ctgtttttat cgcctgaaat
    10081 ttctatgaaa atccaaacgg ttttgaactc ggacatcgcg atgcagttgg acgaatgcac
    10141 gccgggcgag gcgacgcgcg aacaggcgcg aaaatcgttg caaatgagcc tgcgctgggc
    10201 ggaacggagc aaaaaagcct ttgaagattt gaaaaacccg aacgcactgt tcggcatcgt
    10261 gcaaggcgcg atgtatgaag atttgcgcga agagtcgctg aaaggcttgg aagagctcga
    10321 tttccccggc ttggccatcg gcggcttgtc cgttggcgaa cctaagcccg aaatgtaccg
    10381 catgttgcgc gccgtcggcc ctatcctgcc ggaacacaaa cctcattatt taatgggcgt
    10441 cggtacgcct gaagacttag tgtacggcgt ggcacacggc gtggatatgt tcgactgcgt
    10501 catgcctact cgcaatgcgc gtaacggctg gctgtttacc cgttttggcg atttgaagat
    10561 caaaaacgcc aaacacaagc tcgacaagcg tccgatagac gaaagctgca cctgctacgc
    10621 ctgccaaaac ttcagccgcg cctacctgca ccatctgcac cgcgccggcg aaatcttggg
    10681 cgcacagctc aacaccatcc acaacctgca tttctaccaa gtcattatgg cggaaatgcg
    10741 cgatgccata gaacaaggca aatttgccga ctggcaggcg cgattccacg aaaaccgtgc
    10801 tcgcggcaca gactgactcc cggcaatgcc gtctgaagcc gcttcagacg gcattttgcc
    10861 gctttaccct acccgcacta tttttcagaa accgatcatg cttccattct ttccagaacc
    10921 ctccctttcc tatacgcaac aaaaccgtac cgccgttttg ctgttgaacc tcggcacgcc
    10981 cgacgcgccg accgcgcagg cggtacgacc ttatctgaag tcctttctga ccgaccggcg
    11041 cgtcgtcgaa ctgcccaaat ggctgtggta tcccattctg catggtctgg tactgacgct
    11101 ccgccccaaa aaaagcgcgc acgcctatga aaaaatctgg tttaaggaag gttcgccctt
    11161 agaagtttac acagcacgcc aagctgccgc gcttgccaaa cgtatgcccg acctcatcgt
    11221 ccgccacgcc atgacctacg gcaatccttc ggtggcagac gtattgtcgg aactgaaggc
    11281 gcaaggcgcg ggcagactgc tggtcatccc gatgtacccg caatatgccg cttccagcag
    11341 cggagcggca gtagataagg tgtgcgaaca attactgctg caacggaacc aaatgagcgt
    11401 ccgcaccgtt tcgcgctttt atgacgatac cggctacatt gatgcgatga aaaaccacat
    11461 cctccgatat tgggcggaac acggacgtgg gaaaaaactg atgttgagtt ttcacggcgt
    11521 accgcagaag cactacgacc tcggcgaccc ctatcccgac gagtgccgcc acaccgccaa
    11581 actgcttgcc gaagcactgg aactgaccga agaccaatat gtcgtatcgt tccaaagcca
    11641 attcggcagg gcaaaatggg ttacaccgag cacgcaggat ttgttcggca aactgcccaa
    11701 acagggggta accgagctgg acgtattctg cccgggcttt ttggcagact gtttggaaac
    11761 catggaagaa atcgccctga tggggcggga acagttttat gaagcaggcg gcaaaagcta
    11821 ccgctatatc ccctgcctca acgacaaccc cgactggata gacgcactcg tcgcacttgc
    11881 cgaagaaaac cttggcggct ggcgttgaaa ctgtttgacc catgtctaaa atgccgtctg
    11941 aacgccgtgt tcagacggca ttttcccgct actttagcgt atgatggcat ctatgctgga
    12001 caacacatca agtgcaacct tactaccgat tcggcaagaa aatctttcac ccgctacgca
    12061 aaatcaacaa caaggtttca aacattaagg aaacaccaat gaacacaacc cgactgccga
    12121 ccgccctcgt cttgggctgc ctctgcgccg ccgcttctgc cgccgacaac agcatcatga
    12181 caaaagggca aaaagtgtac gaatccaact gcgtcgcctg ccacggcaaa aagggcgaag
    12241 gccgcggaac catgtttccg ccgctctacc gctccgactt catcatgaaa aaaccgcagg
    12301 tgctgctgca cagcatggtc aaaggcatca acggtacaat caaagtcaac ggcaaaacct
    12361 acaacggatt catgcccgcc actgccatca gcgatgcgga cattgccgcc gtcgccactt
    12421 atatcatgaa cgcctttgac aacggcggcg gaagcgttac cgaaaaagac gtaaaacagg
    12481 caaaaaacaa aaaaaaacta aacagacaaa atgccgtctg aaaccggcaa tccggcttca
    12541 gacggcattc aaatcaaacc tttctaacgc ggtactttca gccccataga cccgatattg
    12601 ccgtccccgt caacctcctt cgcggtcagc ataaagccgc cgatgtcaat acggccgcac
    12661 tcgaccggct cctgactgtc ggaacgctta tcgaaaagct cggcaaggct caaaccctct
    12721 tcgcccgctt ccagcttcaa accgtaagca agtgccaaat cgtccgaacg tgctgccggc
    12781 gaaacgacaa actcgccgaa gaaatcaaaa tgctcacgga caccgatatc cgtctcggta
    12841 aagtatttcg acattttata gtgaactaaa tttaaaccag tacggc
//
LOCUS       DAWFJR010000052        12858 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWFJR010000052 DAWFJR010000000
VERSION     DAWFJR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12858
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.052"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(76..1503)
                     /locus_tag="WGR04_001332"
     CDS             complement(76..1503)
                     /locus_tag="WGR04_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ0794876.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPG
                     FEILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFIT
                     IFPVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNM
                     AGGDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGY
                     MMWSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(1861..2220)
                     /locus_tag="WGR04_001333"
     CDS             complement(1861..2220)
                     /locus_tag="WGR04_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ0794877.1"
                     /translation="MIRYLLIACGGISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2217..2822)
                     /locus_tag="WGR04_001334"
     CDS             complement(2217..2822)
                     /locus_tag="WGR04_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ0794878.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDTSAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2869..3351)
                     /locus_tag="WGR04_001335"
     CDS             complement(2869..3351)
                     /locus_tag="WGR04_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ0794879.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3555..3652
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3863..4963
                     /gene="gcvT"
                     /locus_tag="WGR04_001336"
     CDS             3863..4963
                     /gene="gcvT"
                     /locus_tag="WGR04_001336"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ0794880.1"
                     /translation="MITLKTTPFHQAHQDAGAKLVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYCIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVNNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLDKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEVDVRVLKLPFVRNGQKQFD"
     gene            5125..5511
                     /gene="gcvH"
                     /locus_tag="WGR04_001337"
     CDS             5125..5511
                     /gene="gcvH"
                     /locus_tag="WGR04_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ0794881.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDPYGAGW
                     FFKIKPANPADYDGLLTAEQYAGEVD"
     gene            5663..6910
                     /locus_tag="WGR04_001338"
     CDS             5663..6910
                     /locus_tag="WGR04_001338"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ0794882.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPKAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYALDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEIRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIRALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            7187..7612
                     /locus_tag="WGR04_001339"
                     /pseudo
     CDS             7187..7612
                     /locus_tag="WGR04_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            7618..8847
                     /locus_tag="WGR04_001340"
                     /pseudo
     CDS             7618..8847
                     /locus_tag="WGR04_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            8905..9525
                     /locus_tag="WGR04_001341"
     CDS             8905..9525
                     /locus_tag="WGR04_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0794883.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVNQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            9536..9667
                     /locus_tag="WGR04_001342"
                     /pseudo
     CDS             9536..9667
                     /locus_tag="WGR04_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            9664..10158
                     /locus_tag="WGR04_001343"
     CDS             9664..10158
                     /locus_tag="WGR04_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ0794884.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(10246..11907)
                     /locus_tag="WGR04_001344"
     CDS             complement(10246..11907)
                     /locus_tag="WGR04_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ0794885.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEKNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGILVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(12037..12579)
                     /locus_tag="WGR04_001345"
     CDS             complement(12037..12579)
                     /locus_tag="WGR04_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0794886.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTEEDVL
                     KKLDKEQMPASFEDMRRIMPDLGFEAKGYALSFEQLAQLQIPVIVYLKYRKDDHFSVL
                     RGIDDNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKGRDAGGDKAFFT
                     RSPKRQTEFAVGQVKWWRAY"
     gene            complement(12608..12720)
                     /locus_tag="WGR04_001346"
                     /pseudo
     CDS             complement(12608..12720)
                     /locus_tag="WGR04_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         3162 a         3259 c         3470 g         2967 t
ORIGIN      
        1 tgtcggaagg gaaaaatatt gatttgatta tgccggaatt taaaatgccg tctgaatgtt
       61 cagacggcat agcgtttaca gcagtttgaa aacgaaaaag ataagggtat gtacgatgaa
      121 gacgggtgtc aggaaggcga ccgaccacat catatagccg aagaaagtcg gcatcggtac
      181 gccgcgctgt tcggcaatgg ccttgaccat gaagttcggt gcgttgccga tgtaggtcag
      241 tgcgcccatg aataccgaac ccatagaaac cgccagcagc gaatgaaaca gggtacccgt
      301 catcaaggct tgggcatcgc cgcccgccat attgaaaaaa acgagataag tgggcgcgtt
      361 atccaagaat gccgacaata tgccgctcat ccaaaaatac atcacattaa tcggatgacc
      421 tgccgtatcg tgaaccagcg ataccacccc gcccagcgcg cctgcctcgc ctgctttcag
      481 aatgctcagg acgggaaaga tggtgatgaa gatgccgagg aagagtttgc ccacttcggc
      541 gatgggttca aagttgaatt cgttgcctgc gcggacttgt ttgggcgtga ttgccataga
      601 tacggcggtc aatgcaatca ggatgacatc gcggacgagg ttttgcaggg cgtaacggct
      661 gccgaggatt tcaaatcccg ggtgttcggg tttccaaagg ccggacatta gaaccgcgcc
      721 gaccacgccc gaaagcagga ggaagttcca tttgccgaag atggcgattt tttcgggttt
      781 ttcctgttgt tgttgtgccg gcgtatcttg tgcaatgctt tcctgtttga agaaacggtt
      841 gtcgatgaaa tagaaggcgg tcaacaggac agcggtgctg atcaggacgg gggcgaacat
      901 atgtttgacc gtccacatga aatctacgcc tttgaggaag ccgaggaaga gtggggggtc
      961 gcccaaaggg gtcagaccgc cgccgatgtt tgcaaccagg aaaatgaaga agatgacgat
     1021 gtgcacgcgg cgggtacggt tttggttggc tttcagcagc ggacgaatca tcagcattgc
     1081 tgcgccggtc gttcccatga tagaggcaag tgccgtaccg acggcaagca gggcggtgtt
     1141 gagcttgggt gtgccgttca agtcgcccca aaccaaaatg ccgcctgaaa tggtgtacag
     1201 ggcaagcagc agcaggatga aggggatgta ttcttcaacg agtgcgtgtg cgacggtatg
     1261 gataccggcg gacgcgccaa aaaccaaact gaacgggatg aggaagagca atgtccaaaa
     1321 ggcggtgatt ttgccgtaat ggtgatgcca ggtatgcgaa aaaaacaagg gacccaatgc
     1381 gatagacagc aaaatcaggg caaagggcag gctccacagc aggtttaggt ttgcgccgtc
     1441 caaatctgcg gcgtaaaccg atgcggggaa aagcattagt gaaaacaggg gtaggtggcg
     1501 catcgtgttt cctcgattca agcactgcct tgcgcggcgc gtgggagtga tacaggcacc
     1561 gtgccgcccg gacataggcg gactgtgctg cctgtttgtt tttgaaaaaa gttttccgat
     1621 tgattgtaaa gtaaataatc aggataaacc agtttcccaa accggaaggc ggcgggaagg
     1681 cggattgctg tgcttgggaa tatatgttct ttttgataaa taattttatt taaaacaaat
     1741 aaatatatgt ctttaataaa taatctatcg aaaacgaaaa atgaatttat tttaacatat
     1801 atttgcaatg aaacaggttt gccccccgtt tgtttgccct tatccctttc agtacggcat
     1861 tcaagattcg ggcctgcgcc acatccatat ggcgacaagg gaacaaaaaa ccgatgaaac
     1921 cgccccgacc caccagcgtt ggggaaactg ccaaaacatt atcaggcagg atgcggtcat
     1981 catactgatg gcgaatattt tggctttgcg cggcactgcg ccgttttgtt cccagttatg
     2041 aaccatcgga ccgaaatagc ggtgccggtg cagccagcgg tgaaagcgcg gggatgcctt
     2101 tgcccagcag gcggcggaga gcagtacgaa cggcgtggtc ggcaacagcg gcaaaaaaat
     2161 gccgatgata cccaacagca gggaaatgcc gccgcaggca attaaaagat aacgtatcat
     2221 tttgaaatat ttttcttatt gtgcggataa gggcaggatg tgataccgag ttttgcccag
     2281 ccttcatgtc ccattttttc cagcagggcg atattgcgtt cgaatattgc cgaagtgtcg
     2341 ggaaaggctt gtgcggcttt ggcaatgctg tcttcgcgga tgaggtgcag cgtcggatag
     2401 ggagaacggt tggtgtagtt gccgatgtcg tcgggttcgg tattctgaaa gcggaattgg
     2461 ggatggaacg gtgcgatttg gacgatgcct tccaagccgt tttcgataac ggcggcatcg
     2521 gcaatatcga gcatatcgtt gaatacgtcg aaatcgggga atagggtcgg gtgaaccagc
     2581 agggtggttt ccagttcggt ggcgggtgta ttgcccagtc gctgcagttc ttcgtccaag
     2641 tcttccaaaa aactgtcaag gtgtttggct tcgctgattg cgatgcggac aaggttttta
     2701 acgtgggggg ctttggcaaa gggacacagg ttcagaccga tgacggcttt ttccaaccat
     2761 tgtccggtgt gttcggcaac agcatcttta ttttcggaag tattgatatt cattattgtc
     2821 atgtaaatgt gtttgcagat tgcacgtgcg ggaaaatcgg gaagggcact attccttcag
     2881 caggtggttg agcggcaggg aggtggtgtg tttgatttct tttaaaacaa agctcgattg
     2941 cgcatcttgt acgccgtggt gggacaggag cgtatccaaa acaaaatggg aaaacgcgtt
     3001 catatcggta aaaaacgcct gaagcaggta gtcggtttcc cctgtcaggg cgaagcagct
     3061 caagacttca ggccattttc gaaccgatgc ggcaaagtct tcccgcgcgt cttttgcttt
     3121 gcggatggaa acgcggataa atgcctgaag ccccaggttg acggattccg gagacagcag
     3181 cgcggcatat tggcggacga tgccggcatc ttccaactgc ttcagacggc gcaggcacgg
     3241 agaaggcgaa agtgcgacac gttcggacag ttcgacattg gtcagcctgc cgttttcctg
     3301 gagaacctgt aagattttaa tatcggtttt gtctaaagtg agttggggca tatttgcgtt
     3361 ccgttttaag gaattcggat tgtctgtccg tatgtttgcg gcaatccgca cagatggaga
     3421 ccatattaac atataaaaag ttataccgtc atccgggaca aattttgttt tcggaaaatc
     3481 atgtgaaaac agaggcggtc ggtttgcatc tcttttaaga cggcttgccc aaaccgccga
     3541 ttcaagacat aatcgggaaa tgtgcaggag agtgttacac ccaactacaa tgtaaccacc
     3601 gaaggcgcag acacccttaa atcgctcagg tatcagggac tgcacattga aacaaacaat
     3661 ctggagagcg gcgttggaat aacgtccacc gaaggggaga aggccgtctg aaccaccatt
     3721 cagacaaccg cgcaaagcag tgagcagact ggtttgccat catgcggata cggccgaaaa
     3781 tctcaggttc aaggacagat agggtcatcc gcgcacaggt gcgcgggtgg catctgaaca
     3841 aaaaatccgg agaaacttga gaatgattac tctgaaaacc accccatttc atcaagccca
     3901 tcaagatgca ggcgcgaagc tggtagattt tgccggctgg gagctgccca tccattatgg
     3961 ttcgcaaatc gccgaacacg aagccgtgcg caccgacgca ggtatgtttg acgtatccca
     4021 tatgcttgtt accgacgtag caggcgcaaa tgccaaagcc tttttccgca aattgattgc
     4081 caacgatgtc gccaagctcg cttttgtcgg caaagccctt tattccgctt tgctcaacga
     4141 caacggcggc gtgattgacg acttgatcgt ttaccgcacc aatgaagccg aaacccaata
     4201 ctgcatcgtg tccaacggcg cgactcgcga aaaagatacg gcacaattcc acaaagtcgg
     4261 acaagagttc ggcgtttctt tcaatccgcg ctacgacctc ggcatgctcg ccgtacaagg
     4321 cccgaaagcc atcgagaaac tcctgaccgt caaacccgaa tgggcagatg tcgtcaacaa
     4381 cctcaaaccg ttccaaggcg cggatttggg caacgactgg tttgtcgccc gcaccggcta
     4441 caccggcgaa gacggcgtcg aagtcatcct gcccggcacc gaagccgtcg cattcttcaa
     4501 agccctgcaa gcagcaggcg tacaaccttg cggtctcggc gcgcgcgaca ccctgcgcat
     4561 ggaagccggc atgaacctct acggcaacga tatggacgac gataccagcc cgctcgaagc
     4621 aggcatgggt tggaccgttg atttgaaaga cgaaagccgc gattttgtcg gcaaagctgc
     4681 cttgttggca ttgaaagaaa aaggcgttgc cgtcaaacag gtcggtctgt tgctcgacaa
     4741 aggcggcatc ctgcgcgcgc atatggaagt gttgaccgac aaaggcaaag gcgaaaccac
     4801 cagcggcgta ttctctccaa gcctgaaaca atccatcgcc atcgcccgcg tgccgaaaga
     4861 ctttgacggc gataccgcca aagtgctgat tcgcggcaaa gaagtggacg tacgcgtact
     4921 gaagctgccg tttgtgcgca acggacagaa acagtttgat tgatgcggtt tcagacggca
     4981 ttttcatttc atatgctgtc tgaaagcagg ttttaattgt tgtccgatac ggacgtttgt
     5041 agaaagcatt gaacaaggca tctgtggata ttgattcatg cagatgccgt ctgaaaataa
     5101 cccctatcaa tggagtatca aaccatgagc aacaacatcc ccgccgaact gaaatacgtt
     5161 gccagccacg aatggctgcg ccttgaagaa gacggtacga ttaccgtcgg cattacccac
     5221 cacgcgcaag agctgttggg cgacatcgtg ttcgtcgagc tgcccgaagt cggcgcaaat
     5281 ttggctgccg aagaacaggc cggtgtggtt gagtctgtaa aagccgcgtc cgacgtgtac
     5341 gcgccgattg cgggcgaagt cgttgccgtc aacgaagatt tgccaagcgc tccggaaact
     5401 gccaacagcg acccttacgg cgcaggctgg ttcttcaaaa tcaaacctgc caatcctgcc
     5461 gattacgacg gtctgctgac tgccgaacaa tacgcgggcg aagtggatta aaccgcccgg
     5521 ctgcccgacg gcaaccgccg gacaaacgga aactgcacct tcagacggca tttttgcggt
     5581 cggaggtgca gttttttgtc cgtgttttaa ggaagcagtt aggctataat aacggtctat
     5641 attcatcttt accgattttt tcatgcaact taccgctgtc ggactcaatc atcaaaccgc
     5701 acctttaagc atacgggaaa agctggcgtt tgccgccgcc gccctgccta aagccgtccg
     5761 caatcttgcc cgaagcaatg cggcaacgga ggcggtaatc ctttctacct gcaaccgcac
     5821 cgagctttac tgcgtcggtg attcggaaga aatcatccga tggcttgccg attaccacag
     5881 tttgccgatt gaagaaatcc gtccgtatct gtacgcgctg gatatgcagg agactgtgcg
     5941 ccatgctttc cgcgtcgcct gcggcttgga ttcgatggtg ttgggcgagc cgcagatttt
     6001 aggacagatt aaggatgcgg tcagggttgc tcaagagcag gaaagtatgg gtaagaaact
     6061 caatgccctg ttccaaaaaa ccttttccgt tgctaaagag atccgtaccg ataccgctgt
     6121 cggcgagaac tcggtttcca tggcttccgc ttccgtcaag ttggcagagc agattttccc
     6181 cgacatcggc gatttgaatg tcttgtttat cggtgcgggt gagatgattg agctggttgc
     6241 cacttatttt gccgccaaaa gtccccggct gatgacggtt gccaaccgga ctctggcgcg
     6301 tgcacaggag ttgtgcgaca agctcggtgt caacgccgaa ccgtgcctgc tgtccgatct
     6361 gcctgccatt ctgcacgatt acgatgtggt ggtttcttca acggcgagcc agcttccgat
     6421 agtcggcaaa ggcatggtgg agcgtgcatt gaaacaaagg cagagtatgc cgttgtttat
     6481 gcttgacttg gccgtgccgc gtgacattga ggcggaagtc ggcgatttga acgatgccta
     6541 tctttatacg gtggacgata tggtcaacat cgtccaaagc ggcaaggagg caaggcagaa
     6601 ggccgccgcc gccgccgaaa cgctggtgtc cgagaaggtt gccgaatttg tcaggcagca
     6661 gcagggcagg cagagcgtcc ccttgattag agccttgcgg gacgagggcg agaaagcgcg
     6721 caagcaggtg ttggaaaatg cgatgaaaca gcttgccaaa ggcgcaacgg cagaagaggt
     6781 tttggaacgg ctgtccgtcc aactgaccaa caagctgctg cattcgccga cccaaacttt
     6841 gaataaggcg ggggaagaag ataaggattt ggttcacgcc gtcgcgcaga tttatcattt
     6901 ggacaaataa cggtgcgccg gaaaaggatg ccgtctgaag gggtttcaga cggcatattt
     6961 ttgtgctgtc tgacaacatc gtgaaatccc acattatatc gatgtaatca caaagtatag
     7021 tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacagat agtacggcaa
     7081 ggcgaggcaa cgctgtactg gtttaaattt aatccactat attatcccgt atgcggattg
     7141 gttttaagat ttgtaaattt gatttgcatc aaaaaatcgc cgatagatga ttcatataat
     7201 atcaatatta aagagtatcg gtatatcggg gatagtcatg tcctgttttt caatcaaacg
     7261 tatgtccgcg tttcgggcgc ggataacggc gttttttgcc gcctttgtct ttttgacggc
     7321 ggcactgccc gcttatgcgg agcgtctgcc tgattttctg gcgaaaatac agccttcgga
     7381 aatttttccg ggtgcggacc gttacggcaa gccggaaggt aagcctatgg ttgcccgcgt
     7441 ttacaaaggc gatgagcagt tgggcttggt ctatatcacg accgatgcgg tcaatacgcg
     7501 cggttattcg agcaagccga ttgatacgct gatggcgctg gcaaacgacg gcacgatagc
     7561 cggggcgaaa ttggttgatc accatgagtc gattatgctg atcggtatcc cgcatttgcc
     7621 cgcgcccggg cgggcgatac gctcaaactg gcttccggcg tatatcaaac caaacttcac
     7681 atcgacaaac cgattacgat tgaagggcct gccgaccgtt ctgcaaccat cgaaggggac
     7741 aggagcggtc gtacgatagc tgtgcatgcg ccggacgtaa cgctccgcaa cctgaccatt
     7801 acacgttccg gtatgagcct gcccgcaatg gatgccggta tttatctcga agaaactgcc
     7861 ccgcgcgccc tgattgaaca caacaatatt ttggataatt cggtcggcgt ctatctgcac
     7921 ggttctgccg atgcgatggt gcgcgagaat aaaatcgtcg gcgacgcgac tttgcgcgtg
     7981 aacgagcgcg gcaacggcgt taccgtttgg aacgcacccg gtgcgcaggt cgtcggcaac
     8041 gacatttcca aagggcggga cggcattttt tccaacacca gcacgcacaa cacctataaa
     8101 aacaaccgct tcagcgattt gcgtttcgcc gtccactata tgtacaccaa cgacagcgaa
     8161 gtcagcggca atatttccgt gggcaacaat atgggctatg tgctgatgtt ttccgaacgg
     8221 ctcaaagtat tcgacaatat tgccgtcggt agtcgcgact agggcatcat gctcaactat
     8281 gtcaactatt ccgatattca cgacaatatt atcaacaaag cgggcaagtg cgtttttgcc
     8341 tacaatgcca actacgataa actgtccgcc aatcattttg aaaactgcca aatcggcata
     8401 cactttaccg ccgccatcga aggcacgtct ctgcacgaca attcctttat caacaacgaa
     8461 agccaggtca aatacgtcag cacgcgcttt ctcgactgga gcgagggcgg acacggcaac
     8521 tattggagcg acaacagtgc gttcgatttg aacggcgacg gcttcggcga cagcgcgtac
     8581 cggcccaacg gcatcatcga ccaaatcatc tggcgcgcgc ccgtattgcg cctcttgatg
     8641 aacagtctcg caatcagcat cgtcaaatgg gcgcaggcgc agtttcccgc cgttctgcct
     8701 ggcggcgtgg tggacagcaa accgctgatg aagccttatg cccccaaaat tcaaacccgt
     8761 tatcaggcga tgaaggacga gctgctcaaa gaagccgaaa cgcggcagtc ggaatggggc
     8821 agggcggaaa acggttcttt gaactagtct gcttcagacg gcatccggat tcaaatgccg
     8881 tctgaaaaca caaaaggaac aaccatgacc acacatcatg tcgaattgag gaaggtaacc
     8941 aaacgcttcg gcgcgcaaaa agccgtcaac caagtcgatt tggttttgaa ggcaggagaa
     9001 agcgtcgggc ttgccggaca caacggtgcg ggcaagtcca ccatcatgaa gctgatactc
     9061 gggctgatta ccccgaccga aggcgaagtg atgcttttgg gcgaacgtac cggtagcaaa
     9121 gcgggggcgc ggcttcgcag ccaaatcggc tacctgcccg aaactgttgc gctgcaccct
     9181 tcgctgaccg gcatcgaaac gctggatttt tacgccaaac ttaaaaaaca gccgctcacg
     9241 cagaaccggg ggctgctcga gcgcgtcggt atttcacagg cggcacaccg ccgcgtcggc
     9301 acttattcta aagggatgcg ccaacgcctt gccttggcac aagccctgct gggcgagccc
     9361 aaagtcctgc tgtttgacga accgacaacc ggtcttgacc ctgcatcacg acaaatgttt
     9421 tacgaagtcg tgcgcgaact caacgggcgc ggcgcgaccg tattgctcag cacccacgcc
     9481 cttgccgagt tggacgggca cgccgaccgc attatcgtgg attaaattta atccactata
     9541 tgcgggtatg gcgggtttga gcggacaaat cagcctgacc gtccccgttt tgctgaccgc
     9601 tcaggtttta tgggttatca ttccgcttgt tttggcagcc ggaattttta gaaagcgaca
     9661 aatatgaaaa aaaccctgtt ggcaattgtt gccgtttccg ccttaagtgc ctgccggcag
     9721 gcggaagagg ggccgccgcc tttaccccgg cagattagcg accgttcggt cggacactat
     9781 tgcagtatga acctgaccga acacaacggc cccaaagccc agattttctt gaacggcaaa
     9841 cccgatcagc ccgtttggtt ctccaccatc aagcagatgt tcggctatac caagctgccc
     9901 gaagagccta aaggcatccg cgtgatttac gttaccgata tgggcaatgt taccgattgg
     9961 acgaatccca atgccgacac ggagtggatg gatgcgaaaa aagcctttta cgtcatcgac
    10021 agcggcttta tcggcggtat gggtgcggaa gacgcgctgc cgttcggcaa caaagagcag
    10081 gctgagaaat ttgcaaagga taaaggcggt aaggttgtcg gtttcgacga tatgcctgat
    10141 acctatattt tcaaataata ttgaaaaaac cggccgaaac tttaaaaacg agtttcggtc
    10201 ggtttttctt ttcttgttcg gtatagtgtc ggcaggaaag aaccttcaca tcccgccgta
    10261 attcggcccg ctcgcgcctt cgggacaaat ccaagtgatg ttttgcgtcg ggtctttgat
    10321 gtcgcaggtt ttgcagtgta cgcagttggc ggcgttgatt tgcaggcgcg gattgccgtt
    10381 ttcttcaaca atttcgtaca cgccggccgg acaatagcgc gtttcgggcg aggcgtattc
    10441 tttgtagttc acgtctatca tcgtttgcgg attgttcagc accaaatggt cgggctggtt
    10501 ttcttcgtgc gcgagattgg caaggaagac gctgctcaag cggtcgaagg tcaacacgcc
    10561 gtcgggtttc ggataatcaa tcggcttgca cgcggcggct tttttaagct gctcgttgtc
    10621 tttgccgtga tgtttcaagg tccacggggc tttgcctctg aaaatcatct gatcgatgcc
    10681 ggtgtagatt gagccgaggt aaacgcccca tttgaatgac ggacggacat tgcgcgcggc
    10741 gtaaagctct tgatacagcc agctttgttc aaaacgttgc tgataatccg ccgcctcttt
    10801 gccgctgtcg aaaccctcca cttcttcaag gttttccaac aaggggaaca cggcttcggc
    10861 ggcgagcatg gcggatttca tcgcggtatg aatgcctttg atgcgcggca tattgaggaa
    10921 acccgccgca tcgccgacca aaatgccgcc tttgaacgag agcttcggca aactttgcaa
    10981 accgccttca atcagcgaac gcgcgccgta agcaatgcgg cggccgcctt caaaggtttt
    11041 gcggatttcg ggatgggttt tgaaacgttg gaactcttca aacggcgaca gataaggatt
    11101 ttgatagtcc aaaccgacca cgaagccgac ggcgactttg ttgtcgtcga aatggtaaac
    11161 aaacgcgccg ccgtaggttt tgctgtccag cggccagcct gcgctgtgca ccaccaaacc
    11221 gggctgatgc tgttcggacg gcacttccca aacttcttta atgcccaagc cgtaagtttg
    11281 cggctggctg ttttggtcga gttggaaacg ttcgatgatt tgtttggaaa gcgaaccgcg
    11341 acaaccttcg gcaaacaggg tttgctgcgc ccaaagctcc atgccgggtt ggaatgaatc
    11401 ggtcggctcg ccgtctttgc caatgcccat attgccggtt gcaatgcctt tgaccgaacc
    11461 gtcttcgtga tacagcactt cggcggcggc aaagcccgga tagatttcca cgcccatatt
    11521 ttccgcctgc tccgccaacc agcgcacgac ttcgcccaag ctgacgatgt agttgccgtg
    11581 attgtcgaaa ttcggggtaa tcggcaggtt gaacgctttt ttctcggtca ggaacaacac
    11641 tttgtcctgt gttaccgtac gcgtcagcgg cgcgcctttc tctttccaat cgggaatcag
    11701 ctcgttcagc gcaatcggat cgataaccgc gcccgccagc gaatgcgcgc ccgcctccga
    11761 acctttctcc accacgcaaa cgctgatttc gcgcccgttt ttttcggcaa gctgcttgag
    11821 tttgatggcg gcggacaaac ccgacgggcc tgcgccgaca atcacgacat cgtattgcat
    11881 actgtcgcgg gtgatggatt ctgtcatggc ggttcctgtg tatttattat tgaattgcaa
    11941 atccgtaatt atacaacggg aacatatagt taccaaatac aacaaaggtc gtctgaaaac
    12001 catattttcg gttttcagac gacctttgtc gaaatttcaa taagcacgcc accattttac
    12061 ctgtccgacc gcaaactccg tctgacgttt cggactgcgt gtgaaaaacg ccttatcccc
    12121 gccggcatcc ctccctttcg gcacaaccgc caaaatctta cctgccaaat ttccctcacg
    12181 ggtttgccaa gcatccaaaa actgcgccct gctcatcgaa acatgaccca gcgacgggtc
    12241 ggcaagcaaa accgtattgt cgtctatacc gcgcaatacc gagaaatgat catccttgcg
    12301 gtatttcaga tacacgatga cggggatttg caactgtgca agctgctcga aagacagggc
    12361 atagcctttc gcttcaaaac ccaaatcagg cataatgcgc cgcatatcct caaacgacgc
    12421 gggcatctgc tccttatcca gtttttttaa cacgtcctct tccgtcagct tttgcccgta
    12481 aaaattgttc aaaagcgtcg ccaccgaagc cgccccgcag gaaaaatcca aatcctgctt
    12541 tacaatattg aaatcgcgcc tctctttcca actctgcact ttgatttttc cataagcaac
    12601 aggattatag tggattaaat ttaaaccagt acggcgttgc ctcgccttgc cgtactattt
    12661 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat aggtttccgt
    12721 gcggacgtgt tcagattccc gccttcgctg gaataacggc ggagcagttt ctgcttttcc
    12781 gataaatgac cgtaacttaa aatcccgtca tccccacgaa agcaaaaatc ctgcctgtcg
    12841 gatttcggtt tttttggg
//
LOCUS       DAWFJR010000053        12180 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWFJR010000053 DAWFJR010000000
VERSION     DAWFJR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12180
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.053"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(87..509)
                     /locus_tag="WGR04_001347"
     CDS             complement(87..509)
                     /locus_tag="WGR04_001347"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ0794887.1"
                     /translation="MSIMEVEVVSSEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            complement(520..1917)
                     /gene="atpD"
                     /locus_tag="WGR04_001348"
     CDS             complement(520..1917)
                     /gene="atpD"
                     /locus_tag="WGR04_001348"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ0794888.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            complement(1955..2830)
                     /gene="atpG"
                     /locus_tag="WGR04_001349"
     CDS             complement(1955..2830)
                     /gene="atpG"
                     /locus_tag="WGR04_001349"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ0794889.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            complement(2855..4402)
                     /locus_tag="WGR04_001350"
     CDS             complement(2855..4402)
                     /locus_tag="WGR04_001350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ0794890.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            complement(4413..4946)
                     /locus_tag="WGR04_001351"
     CDS             complement(4413..4946)
                     /locus_tag="WGR04_001351"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ0794891.1"
                     /translation="MAEFATIARPYAKALFGLAQEKSQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            complement(4951..5421)
                     /locus_tag="WGR04_001352"
     CDS             complement(4951..5421)
                     /locus_tag="WGR04_001352"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ0794892.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            complement(5492..5728)
                     /gene="atpE"
                     /locus_tag="WGR04_001353"
     CDS             complement(5492..5728)
                     /gene="atpE"
                     /locus_tag="WGR04_001353"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ0794893.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            complement(5785..6651)
                     /gene="atpB"
                     /locus_tag="WGR04_001354"
     CDS             complement(5785..6651)
                     /gene="atpB"
                     /locus_tag="WGR04_001354"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ0794894.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            complement(6641..6994)
                     /locus_tag="WGR04_001355"
     CDS             complement(6641..6994)
                     /locus_tag="WGR04_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ0794895.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAVWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            complement(7362..7700)
                     /locus_tag="WGR04_001356"
     CDS             complement(7362..7700)
                     /locus_tag="WGR04_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794896.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRSNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            complement(7836..8696)
                     /locus_tag="WGR04_001357"
     CDS             complement(7836..8696)
                     /locus_tag="WGR04_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ0794897.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEVKTANHKKGRIVLYFDTPETFGHL
                     LEQLGIDYRP"
     gene            8854..9423
                     /locus_tag="WGR04_001358"
     CDS             8854..9423
                     /locus_tag="WGR04_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ0794898.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            complement(9485..10348)
                     /locus_tag="WGR04_001359"
     CDS             complement(9485..10348)
                     /locus_tag="WGR04_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794899.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAEKKE
                     IVFGTTVGDFGDMVKEHIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(10507..11193)
                     /locus_tag="WGR04_001360"
     CDS             complement(10507..11193)
                     /locus_tag="WGR04_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0794900.1"
                     /translation="MAGLTFEQTVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPVAIVCKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            complement(11196..11933)
                     /locus_tag="WGR04_001361"
     CDS             complement(11196..11933)
                     /locus_tag="WGR04_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0794901.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDTGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALASKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
BASE COUNT         2937 a         3151 c         3107 g         2985 t
ORIGIN      
        1 tgatttttgt taatccacta tattttcaaa acttggaaaa ataaaaagca cgaccaaacg
       61 gtcgtgcttt tccaaaccaa acaagtttat ttcttgtgcg aacggatata gtccaaagtt
      121 ttgagctgtg caatcgcagc agccaatgct ttatgcgcct ccgccaaagc cttatcgtct
      181 ttagcttggg aaatgcccgc ttctgcggct tttttcgcct cttccgcacg tgcctgatcc
      241 atctccgcac tgcggacggc aacatccgcc aagacagtta ctttatcagg ctgtacttcc
      301 aaaacaccgc cggaaacagc aaccaaaacc tctttatcct cgcccggaac ggtcaaacgc
      361 aaagcccccg gccgcaccaa actcataatc ggctcgtgtc gcggataaat accgagttcg
      421 ccctgtacag tcggaacaac gataaatgtt gcctcgcctg aatagatttt ctgctcgcta
      481 cttaccacct caacttccat gatgctcatg ccgacctcct cagtttaagg ttttcgcttt
      541 ctcaaccgct tcttcgatgc tgccgaccat atagaatgcc tgctcgggca gatggtcgta
      601 ttcgccgttc aagatggctt tgaagccggc aatggtatcg cgcagggcga catatttgcc
      661 cggagaacct gtaaacactt cggcaacgtg gaacggttgg gacaggaagc gttggatttt
      721 acgcgcacgc attacggtca gtttgtcttc gtcagacaat tcgtccatac ccaagatggc
      781 gatgatgtcg cgcaattctt tgtatttctg cagggtggac tgtacgccgc gcgccacgtc
      841 gtagtgttct tgacccaaga ccatcggatc cagttggcgt gaagtagagt caagagggtc
      901 aactgccggg taaataccca aagaggcaat atcacggctc aatacgacgg ttgcgtccaa
      961 gtgggcgaac gttgttgccg gagacgggtc ggtcaagtcg tccgcaggta catatacggc
     1021 ttggatggaa gtaatagaac cggtttgggt agaggtaata cgctcctgca aacgacccat
     1081 ttcttcagcc aatgtcggtt ggtagcccac tgcagaaggc atacggccca acaatgcgga
     1141 tacttcggta ccagccaggg tgtaacggta gatgttgtcc acgaagaaca atacgtcgcg
     1201 gcctttgccg ttttcgtctt tttcgtcacg gaagtattcc gccatggtca aaccggtcaa
     1261 tgcgacgcgc aaacggttgc ccggaggttc gttcatctga ccgtaaacca ttgccacttt
     1321 atccaatacg ttggaatctt tcatctcgtg gtagaagtcg ttaccttcgc gggtacgctc
     1381 acccacgcct gcgaacacgg acaagccgct gtgcgctttg gcgatgttgt tgatcaattc
     1441 catcatgttc acggttttac ccacaccggc accgccgaac agacctactt taccgccttt
     1501 ggcaaacgga cacagcaagt caatcacttt aatgcccgtt tcgagcaatt cggttgtgga
     1561 agacagttcg tcaaacttag gggcggcttg gtggatggca cggctcttgt cggtatcaat
     1621 cggacctgct tcgtcaacag gcgttcccaa tacatcgaca atgcgtccca acgtaccttt
     1681 acctaccggc acagtaatgg gcgcaccggt attgctcaca gtcatgccgc gtttcaaacc
     1741 gtccgagctg cccatcgcaa tggtacggac tacgccgtcg cccaaaagct gttggacttc
     1801 caaagtcaga ccgttttcgt ctaatttcaa agcgtcgtaa acgcgcggaa tcatgtcgcg
     1861 tggaaattcc acgtcaacaa ccgcaccgat aatttgtacg attttgcctt ggctcattat
     1921 cgtatcctaa tttctgtaca ggattcagac ggcatcagac agccgccgca cctgctacaa
     1981 tttctgacaa ttccgtggta atcgcagctt gacgcgattt gttatatacc agacgcaact
     2041 ctttgatggc attgcctgca ttgtctgttg cagctttcat ggcaaccatg cgggctgcct
     2101 gttcggatgc catattgtcg ctcaacgcct gataaaccac agactctaaa tagcggcgaa
     2161 ccagatattc caacactgca agtgcagtcg gttcgtagcg gtattcccag ctgaacggtg
     2221 atttgggagc tgaatcgcca atcacgttct caccgatagg cagcaatact tccattctcg
     2281 gttcttgacg catggtattg acaaaacccg aatacaccag atggattctg tcaatttcat
     2341 gtttctcata ccgttggaag agttctgtca aaggtccgag cagcatttcc atttttgggg
     2401 tatcgcccaa atttacggca ctggcaacca cattcagacc aatgctctga cacgccatca
     2461 gacctttact gccaaggcat acgatttcct cttcaatacc ttgattccga tactcttgaa
     2521 cttgtgccaa aaactttttc agcacgttgg cgttcaaacc gccacacaaa cccttatcag
     2581 acgtaatcaa aataaaaccg acacgtctaa tctcccgatg agattccagt aacggaatac
     2641 catgatcggt attggtttgc gcaagatggc tcatcaccat acgcactttt tcggcatacg
     2701 gacgcgccaa acgcatccgt tcctgagtct tccgcatttt agaggttgac accatctgca
     2761 tcgctttagt gatcttttgg gtattctgaa cactgcggat tttggtgaga atctcttttc
     2821 ctactgccat ttcagactcc tttcacttca agccttatgc ctgataggcg taagaagatt
     2881 tgaaggattt catggctgct tcaagcgttt tctcgctctc gtcggacatt gcacctgaag
     2941 cattgacggc ttccaaaact tccggatgtt gggtacggac aaagctcaaa aattcagatt
     3001 caaaagccag agctttggca accggaacat cagaatacga accgttgttg attgcccaaa
     3061 gggtcaaagc catttcagcc gtattcaacg tactgaactg tttctgtttc atcagttcgg
     3121 ttacgacttc gccatgctcc aattgtttgc gcgtagcttc atccaaatcg gatgcaaatt
     3181 gcgagaaagc cgccaattca cgatattgcg ccaacgccaa acggataccg ccacccagct
     3241 ttttaatcac tttggtttgt gcagcaccgc ctacgcggga tacggaaata ccggcattga
     3301 ttgcaggacg gataccggcg ttgaagaggt cggtttccaa gaaaatctga ccgtcggtaa
     3361 tcgaaatgac gttagtcgga acgaaagcag atacgtcgcc cgcttgggtt tcgataattg
     3421 gcaaagcagt cagagaaccg gttttgcctt ttacttcgcc gttggtcaat ttctccactt
     3481 cgtgttcatt gacacgtgcc gcacgttcca acagacggga gtgcaggtag aacacatcgc
     3541 cgggataggc ttcgcggccg ggcggacggc gcaaaagcag ggaaatttga cggtaagcca
     3601 cagcctgttt ggacaaatcg tcataaacaa tcaaggcatc ttcgccgcgg tcgcggaaga
     3661 attcacccat cgtacaaccg gagtaaggtg cgatatattg caatgccgcc gcttcagatg
     3721 cagttgcagc aaccacaatc gtgtgttcca tcgcgccatg ctcttccaat ttgcggacca
     3781 cgttggcaat agaagatgct ttttgaccga tagcgacata gatacagata acacccgtac
     3841 ctttttggtt gacgatggca tccaatgcta cggccgtttt accggtttga cggtcgccaa
     3901 taatcaactc acgctgaccg cgaccgacag gaaccataga gtcaatcgcc ttcagaccgg
     3961 tttgcatcgg ctggtcaacc gatttgcgcg caatcacgcc cggtgcgatt ttttcgatag
     4021 gggcggtcaa agttgtatta atcgggcctt tgccgtcgat aggccgaccc aatgcatcaa
     4081 cgacgcgtcc gaccagttcg cgtccgaccg gcacttccaa gatacgaccg gtacaggtaa
     4141 ccgtgtcgcc ttctttaata tgttcgtact cgcccaacac tacggcgccg acggagtcgc
     4201 gctccaggtt catcgccaag ccgaaagtgt tacccgggaa ttcgagcatc tcaccttgca
     4261 ttgcatctga caaaccatgg atgcgaacga taccgtcagt taccgaaatc accgtaccac
     4321 gggtacgcac ttcggcattt acagacagat tttcgatctt ggctttaatc aaatcgctaa
     4381 tttcagcagg attaagctgc atgaaaactc tcctaattcg tcatagtcgt gtacaaggca
     4441 ctcaatttgc cttgtacaga caaatccaaa acctgatcac ccacttcaac ttttatgccg
     4501 ccaatcagct ccggttcgat ttcgacagag attttcagct cgctgtcgaa acgcttattc
     4561 agcatttgcg ccaactcgcc gacctgtttg tcggtcaacg gataggcact gtaaatgacg
     4621 gcagatttga tatggttgaa tgataaggtc aagtcttgat attgagcata tacttccggc
     4681 aatatcgaca aacgtttctg cccggccaag acgataacaa agtttttcaa ctccttgtct
     4741 ttcaaaccga ccaaatcgat gaggatatct gctttttctg aagcattcgt ttcaggacgg
     4801 tcaatcaatg aagccacctt cccttcctga acaaccgccg caagtttttc cagtccgccc
     4861 aaccaagact caatttggct tttttcctga gccagaccga acaatgcctt tgcataaggt
     4921 ctggcaatcg ttgcgaactc tgccataaga ttacagctcc tgtttcaggg tatcgagcag
     4981 ttttgcgtgt ttggaagcat cgacttcgct gcgcaaaata gattcggcac ctttgacagc
     5041 caacacggca acctgctcgc gcagggattc gcgtgcgcgg aacaattcct gctccacatc
     5101 ggcctttgcc tgagctgcaa tgcgcgccgc ctcggaagaa gcctgttctt tggcttcttc
     5161 gacaattttg gcggcacgtt tttcggcgtt ggcaaccatt tcggaaacct gattacgccc
     5221 ttctgccaag agttctgcaa cctttttttc agcctgctcg aaatcgcttt taccacgctc
     5281 ggcggcagcc aagccttcgg cgacttttgc ggcacgctca tccaaagctt ttgcaatcgg
     5341 cggccacacg aatttcatgg taaaccatac caaaccgaaa aagacgatga tttgagcgaa
     5401 taatgttgca ttgatattca cgttacttaa ccttcgtact ggggttaatc aaacaggctg
     5461 cgcctgtacg gaacggacga atccgtcctg attatgcacc tgcaaacggg ttaacgaagg
     5521 cgaacagcag tgcaatggcg acaccaatca agaatgcggc atcaatcaaa ccggcaatca
     5581 ggaacagttt ggtttgcagc ggaccgatca gttcgggctg acgggcagaa gactccaaat
     5641 atttagaacc gaccattgcg ataccgatag aggcacccaa tgcacccaat gcaacgatca
     5701 aaccacatgc gatagcaatc aaacccattt taaactcctt aaagaaacaa aggttaaact
     5761 acaaaaacaa actacttagg aaaatcagtg cgcatcatgt gcctgtccga tatagacgaa
     5821 cgccaacgcc atgaaaataa acgcctgcag ggtaatcacc aaaatatgga aaatcgccca
     5881 tgccaaaccg gcaataatgt ggaatacaaa cagaatcgga tccatgactt cgacgctgcc
     5941 ggaagccgcc caagcaccgc caagcaaggc tatcagcaag aataccaatt cgcccgcata
     6001 catattgccg aacaaccgca taccgtggga tacggtttta gaaagaaact cgaccaaatt
     6061 caacagaaag ttcgcaggtg cgagttttgc accgaacggc gcgctgaaca actcgtgaaa
     6121 ccagccaccc aatcctttga ttttgatgtt gtaatagata caaatcagca acacgccgac
     6181 agcgagtgcc aaagtggtgt tcaaatcggc agtcggtacg acgcgcagca gggcgtgatg
     6241 gttgccggta atgccctgcc ataccatcgg cagcaaatcg accggcagca tatccatcgc
     6301 gttcatcaga aaaatccaga caaacagcgt cagacccaac ggcgcgacgg cttttctaga
     6361 cttttcgttg tgaatgatgc tcttacacat atcgtccaca aactcaaaca agatttccac
     6421 ggcagcctgg aaacgtccgg gaacgcctgc cgttgctttt ttcgcaccgc gccacaacag
     6481 gaagctgccg attacgccca acaggacggc aaaaaagacg gcatcaaggt taataaacga
     6541 aaaatcagca atgtttttca gtccctgacc ctgagtaaca tccgacaaac tggtcaagct
     6601 ctgcaagtgg tgcttgatgt agtcggcagc ggtaatggtt tcacctgcca taatctttca
     6661 ctctcaacaa tactaaaaaa accaaatggc tgacaccgag cagccccatc agaaacgggg
     6721 cgaacaccaa cgattgatgc catacagcaa atacggcgag catggacaac agcgacagca
     6781 ctacttttaa aatctctccg aagacgaaca tcctgctttg caggaagggg tttcccctga
     6841 aaagttttaa aagtaaaact gcaacaaacg tgggaagcag gtaggacaaa ccgccaccga
     6901 ccgccgaaag gaatccggca aaaccccata cagcaaaggc aactgcggcg catatggaca
     6961 atacggcgga ttgtaggatg ataatctgct tcataaaggg aatgtttccg cctcggattt
     7021 ggggcgcggc taatataatt tagaagcctt attacgtcaa gcgacagtta atctttgtga
     7081 aacaacgtat cccaatccgc cgcgctcgcc gcctgaataa cggcgacagg tgtcattcta
     7141 acacacatta catataatta caggatatta aggagtttgt ccgcaatttc tttacacttt
     7201 taatgttctt acgtgattgg tttgctttac gtggaaataa taaaaaatca acgcgaaatt
     7261 gtagcagttt atcggtcgga ttgtcggcag tttggggaat ttgctcaata aataaaaggt
     7321 cgtctgaaaa tattttcaga cgaccttttc cgaataaagg attagcaacg gcctgccgct
     7381 ttaagcaaag cattgcattg acttttgcct ttgtgcgttc cgcctcccaa acaaattgca
     7441 tcggaagtgg taacgccgat tgtgctgatt acactggtaa catagcattg gctcacgcgc
     7501 ttacccacag ttgcggtaaa gttgatgcgt atgccttcat tgctgcggtt gctgattttt
     7561 acggcatttg ggctgacgcc caaggcaaac gcggcacgtt cctgaagttt ctggtcggaa
     7621 acggttacat tattgattga gccgcaacct gctaatgcca acgcaacgaa cgcagccgaa
     7681 acgatgatgc gtgtgttcat aatttcctcg aaaattaaaa atgaaaacag gaaaacgatt
     7741 cttacgtgaa gcagaaaaaa tgtcaataga attatatttt ccacttacaa tctggaaagc
     7801 tattctctat atttcagacg gtatatcccg caaaattaag gtcggtaatc tatgcccaac
     7861 tgctccagca ggtggccgaa cgtttcaggc gtatcgaaat acaggacaat cctgcctttt
     7921 ttgtggttgg cggttttgac ttcagcgttg acacccagtt tttcagtcag caaatcattc
     7981 aggcggccga tgtcggcggc ggcagtcttt ttgggctcgg gacgtttgtt ttgaagggcg
     8041 gcttggctgc ggcgctcgac ttcgcgcacc gaccagccgt ttttgacggc cttttgcgcc
     8101 aactcgagct gttcgacgac gggcagggtc agcaatgcgc gggcgtgtcc catttcgagg
     8161 cggcgttggt aaagcatttc ctgcacgggt tcgggcaggc ttaaaaggcg caggctgttg
     8221 gaaatcgcgc ttcggctttt accgacggct tgggcgatgg tttcgtgggt cagtccgaac
     8281 tcgtcggcaa ggcgtttcaa gccttgtgct tcttcgatgg ggttgaggtt ttcgcgctgg
     8341 aggttttcga tcaaacccat tgccaatgcg gtttcgtcgc tgatggtttt gataacggcg
     8401 gggatttcgg tcaggccggc aatctgtgcg gcacgccaac gccgctcccc cgcaatcagt
     8461 tcgtatcggg acagtccgtg ttcgcgcacg atgacgggct gtatcacgcc ttgcgcctta
     8521 atcgaatctg ccagctcctg caaggcttcg tcatcgattt gaacacgcgc ctgatagcgg
     8581 ccgggccgga tatctttaac cgcaaccgtg gtcaatcggt cgccgctgct gttgtctgcg
     8641 ccgttggcga gcagcgaatc caaaccgcgc cccaatccgc cttttacttt tgccataccg
     8701 ccctcccgtg cctattcaga taggatgtta aatcggatat tttatcggat attgggtgtt
     8761 gccgacaatt tgtatccgta tttatcggat ttctgttttt tcactataat agccggtttg
     8821 ccgttgcagg cggttttatg ggaaaggcgg atgatggtac ggcgtttgat aatcggcatc
     8881 agtggggcga gcggtttcca atacggcgtg aaggctttgg aacttttacg cgcgcaagat
     8941 atcgaaacgc accttgtggt atcgaaaggt gcggagatgg cgcgcgcttc ggaaacggct
     9001 tatgcgagag acgaagtgta tgccttggcg gacttcgtgc atccgatcgg caatatcggg
     9061 gcgtgcattg ccagcggtac gtttaaaacg gacgggatgc tggtcgcccc ctgttcgatg
     9121 cggacgcttg cctcggtcgc gcacggcttc ggcgacaacc tcttgacgcg tgcggcggat
     9181 gtggttttga aggaaaggcg gcggctggtg ctgatggtgc gcgaaacgcc gctgaacctt
     9241 gcccatttgg acaatatgaa gcgggtaacg gaaatgggcg gcgtggtgtt tccccctgtt
     9301 cctgcgatgt accgcaaacc gcagacggcg gacgacatag tggcgcacag tgttgcacac
     9361 gctttgtcgc tgttcggaat cgatacgccg gattcggcgg aatggcaggg aatggcggat
     9421 taaaggacaa aaatgccgtc tgaacacgga tacggttcag acggcatcat tttatacgac
     9481 tgccttattt ggctgcgcct tcattccatg cggcagggga tttgtagccc tcgaagcgtt
     9541 tgtgcgcgta ggctttgaac gcgtcggagt tataggcctc ggttacgtct ttaagccatt
     9601 ggctgtcttt gtcggcggtt ttgacggcag accagttgac ataggcaaag ctcggttctt
     9661 ggaacagggc ttcggtcagc ttcatgccgc tgcttatggc gtagttgccg ttgacgacgg
     9721 caaaatccac gtcggcgcgg ctacgcggca gttgcgcggc ttcaagctcg acgattttga
     9781 tgtttttcag gttttcggca atgtccgctt tggatgcggt cagcggattg atgccgtctt
     9841 tgagtttgat ccaacccagt tcgtcgagca tcaccaagac gcgggcgaag ttggacgggt
     9901 cgttgggcgc ggacacggtg ctgccgtctt tgacttcttc cagcgatttc agcttgcccg
     9961 ggtacagtcc caaaggcgcg gtcggcactt ggaagacttc ggtgatgtcc agattgtgtt
    10021 cttttttgaa gtcgtcaaga tagggtttgt gttggaagac gttgatgtcc aactcgccct
    10081 cagccaatgc cagattcggg cgcacatagt cggtaaactc gaccagtttg acggtgtagc
    10141 cttttttctc cagctcgggt tggatgtgtt ctttgaccat atcgccgaag tcgccgacgg
    10201 tcgtgccgaa gacgatttct tttttctccg cgccgttgtc ggcggcggca gaagcggatg
    10261 cggcgggcgc gctgtctttt tgaccgccgc aggcggcgag gatgagcgcg agtgcggcgg
    10321 cggaaagggt tttgaagaag gttttcatat tttctcctga tgttgtggca gtttcaaaca
    10381 aaaatgacgg gcagggagtc ctgccgtcca gattcggcgt tcagacggca tttgccgcga
    10441 acagggggat tttatagcat ttttcggata gcggtggggg ttttggcgtt cagacggcat
    10501 tcgggtttaa cgtttatcca atttccgcgc caatgcgttg ccggtgcttt gaatcaggat
    10561 gaccagcagc acgaggaggg cgacgatgaa gacgatgact tcggtttggt agcggtagta
    10621 gccgtagcgg atggcgaggt cgcccaagcc gccgccgcct atcatccctg ccgccgcgct
    10681 gtatgacaaa agcccgatgg caagcacggt aatgctggaa accatgcccg cgcgcgcttc
    10741 gttcaagagg actttgcaga cgatggcaac cggcggcgca cccatcgcgg cggcggcttc
    10801 aatcacgcct ttggggactt cgcgcaggtt ttgttccacc agtcgggcaa aataaaacaa
    10861 tcccgacacg ctcaacacca gcgaggcggc aaccgggccg atggtgctgc cgacgatggc
    10921 gcgtgtggcg ggtatcatcg caatcatcaa aatgacgaag gggaaggcgc gcatgaggtt
    10981 gacgaggttg tcgagcagga agttcaccgg cttgttgtaa tgcagttggc ggctggaagt
    11041 tacgaagagc aacacgccca gcaacgtgcc gaaaacgacg gcgaatgtgg tggacaaacc
    11101 gaccattacg aaggtttcgc ccaatgcgcg gacgatttcg cctttcatac cgatgatgtt
    11161 ggaaacggtt tgttcaaatg ttaagcctgc catattcagt cctcccgaat cagttcgcgc
    11221 ccgatgtcgg atcgggcgtg gatttggttg ccgcgtactt cgacgatttc gacgactttt
    11281 cccttatcca acagggcggc tcggtcgcac agtcgtctga tgacgctcat ttcgtgggtt
    11341 acgatgacga tggttacgtt gaagcgtttg ttgatgtctt ccaaacattc caagacgctg
    11401 cgcgtggtgg cggggtcgag ggcggaagtg ggttcgtcgg cgaggatgac ttgcggcttg
    11461 gatgcgagcg cgcgggcgat gccgacgcgc tgtttttgtc cgccggaaag ctgggcggga
    11521 tagtgaccgg cgcgttcggt cagtccgacg atttcgagac attcggcaac gcgcgctttg
    11581 attttttcag acggccatcc ggcgatttcc aatgggaagg cgacgttgcc ggcgacggtg
    11641 cggttgttca acaggttgaa ctgctgaaac accatgccga tattctgccg agcctgacgc
    11701 aatgcggcgg catcaagcgc ggtcagctct tgtccacaga cgttgacctt gcccgtgtcg
    11761 ggacgttcca acaggttaat cagtcgcaac agggtggatt tgcctgcacc cgaataaccc
    11821 atcagcccga agatttcacc gtcgcggatt tcgaggctgg tcggctcgac agcggcaaaa
    11881 cgggtcttgt cgcgcgtttg gtaatgcttg gaaaccttgt ccaaaataat cattgtcttt
    11941 cccatacaac aaagcccgat gtcggacaca acgggcgcgg aagataaagc tgaaattgtc
    12001 ggaacgcttt agctgttatg cccgcaagct gtgtcaaatc ggcaggttaa ttttcgtagg
    12061 atattatcgg gaaagcgttt ttttgtcaat aaggcaggaa gcgggcaacc atttcggaca
    12121 atgccgtctg aaacaggcaa aggcagcggt tcgcactaaa acggcaaata attgaaaaat
//
LOCUS       DAWFJR010000054        12175 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWFJR010000054 DAWFJR010000000
VERSION     DAWFJR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12175
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.054"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(1..104)
                     /locus_tag="WGR04_001362"
                     /pseudo
     CDS             complement(1..104)
                     /locus_tag="WGR04_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(477..1145)
                     /locus_tag="WGR04_001363"
     CDS             complement(477..1145)
                     /locus_tag="WGR04_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0794902.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGITNIIGTQLESGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            complement(1142..1810)
                     /gene="hda"
                     /locus_tag="WGR04_001364"
     CDS             complement(1142..1810)
                     /gene="hda"
                     /locus_tag="WGR04_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ0794903.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            complement(1915..2562)
                     /locus_tag="WGR04_001365"
     CDS             complement(1915..2562)
                     /locus_tag="WGR04_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEZ0794904.1"
                     /translation="MSAFFTLFVPCAKTNSPPQENVALFRHNGEILLSGINIKLKSGD
                     SLLIRGPSGCGKTSLLRALAGLWPFGSSGKVSRPPHQDILFLPQRPYTAQGSLRDAVC
                     YPDIDKQHPELIEAMNTCRLGYLVDKLDKTDDWQHKLSPGELQRVAFVRALLSKPKIV
                     LLDEATAALDEPAEAALYRALKQKLPDSIIISIGHRSTLNAFHNMRLDVGNVACG"
     gene            complement(2714..3154)
                     /locus_tag="WGR04_001366"
     CDS             complement(2714..3154)
                     /locus_tag="WGR04_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0794905.1"
                     /translation="MGNPVDLSEFVFVPRYNVAAAAGYGAPVFGEEPLFCLAFRKYWI
                     DNYVTKQPEKLSVITVKGDSMEGVLNHGDSILINHAKTDPRDGLYVLRIGNDLFVKRV
                     QRIPGKLLVTSENPRYAPFEIDFSNAQDDIAIIGRVEWYGRSID"
     gene            complement(3430..3792)
                     /locus_tag="WGR04_001367"
     CDS             complement(3430..3792)
                     /locus_tag="WGR04_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003741237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEZ0794906.1"
                     /translation="MDNSFELSSKLLGERLKKIRTDNGYSREQLSELFGISRASIQNY
                     ENGERSPNADYLVQFYKHFGINLHWLLTGNKAAKFQDFIDSVSSPREEVLLHLARQMD
                     SRTLNHLIDFLMDVYAKK"
     gene            3912..4100
                     /locus_tag="WGR04_001368"
     CDS             3912..4100
                     /locus_tag="WGR04_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0794907.1"
                     /translation="MGIKTVNEVREWLKDNNLTVAQWARENGHNSVDVTRVLTGKSKC
                     RYGQGRAIAIKLGLRIDE"
     gene            4111..6102
                     /locus_tag="WGR04_001369"
     CDS             4111..6102
                     /locus_tag="WGR04_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0794908.1"
                     /translation="MGITIGELLKLNIQGLPTTARGLRERAKTQNWPYIEEVGKARGG
                     RLKKYLIASLPAEIRAAIMKRQSDELTEKMPKTLPQVRPGTAMSAQALAEAAKLLNEK
                     QRSVADARCAVVAAVLGIKYQYGCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSR
                     SAKVGERTLDGWISAYLKAENATERLVALAPKTTKVVKPIESYGWLPMFMQFHNIPSA
                     PKLAHSYRRFVQWAEAENMPVNDVPSLSMVRRVWGKLPLIMQERGRKTGAAYKSLLPY
                     VKRDWGALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAE
                     SCVAVSDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIAGNP
                     QGRGIIERWWKDNLIEMARQYETFASAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQ
                     QKYLKKLPSWSRFIADVVKCIDEYNNRPHGELPRHPDGGHYSPKAYREMRLEQDGIAP
                     DMLSAEELATMFMPQEVRKVQRGWLDLFNNSYFSVELAEYHKDEVRVSYDLSDASVVN
                     VFDMDGKFITKAQANGNTREAFPTARIDQLAENRRKGKIKRAENAIKLANAEVNPALE
                     QAAVWDELGHLGGNVIEAEYAVLPRTGTDDFVLFEADRS"
     gene            6138..7052
                     /locus_tag="WGR04_001370"
     CDS             6138..7052
                     /locus_tag="WGR04_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0794909.1"
                     /translation="MKQINQALQQKLAEFKAKSGMNQTQLARGIGTSPASISMYLNGT
                     YADKGGNYETIEPKIEAFLEMQDSKAQREELVLGFVSTKTTRRIAEVMRDAHEGGETV
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIVRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSVALNLGESLPDDELFEIAKAALPDADEETLLELVKHSNGNTRRMSKLM
                     RGAVRTANKNGIKMQVGIVKKYSSLIIR"
     gene            7143..7409
                     /locus_tag="WGR04_001371"
     CDS             7143..7409
                     /locus_tag="WGR04_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794910.1"
                     /translation="MQVLKKVDWKMFVARSFWRCVPVGLTAGVWCFVAGMALHSCAKE
                     PEPVAKELTKVEKMERQADLEVKVWEEQYESMSVEEKMKGIVYE"
     gene            7518..7691
                     /locus_tag="WGR04_001372"
     CDS             7518..7691
                     /locus_tag="WGR04_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794911.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATARMAQKSREREIEEYQAQFEDKD
                     GKGRLKTGGNKDE"
     gene            7684..7851
                     /locus_tag="WGR04_001373"
     CDS             7684..7851
                     /locus_tag="WGR04_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794912.1"
                     /translation="MNEKDLIEWLEDRGELMVMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            7915..8142
                     /locus_tag="WGR04_001374"
     CDS             7915..8142
                     /locus_tag="WGR04_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794913.1"
                     /translation="MKVYVFKISNENGKLKIELPEIPMGKQIDEVDLIAGLTTEFIAS
                     MLRDAQKDRRKFVIDASNQLAAIQTYQKIFN"
     gene            8157..8678
                     /locus_tag="WGR04_001375"
     CDS             8157..8678
                     /locus_tag="WGR04_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEZ0794914.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAVIEEEYAAKAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFSEFVRTKKEIDKDAILNQKERFSDGQVPGIKIVSGL
                     EDFVIEPTEQELA"
     gene            8678..8923
                     /locus_tag="WGR04_001376"
     CDS             8678..8923
                     /locus_tag="WGR04_001376"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794915.1"
                     /translation="MENGNLNTDELEVLRTAARDSFYIHAQVENANRKLETAFFVWEK
                     VKEGEKEAMRARKKAFIYYCFGVVWFFLALILFFFVV"
     gene            complement(8994..9209)
                     /locus_tag="WGR04_001377"
     CDS             complement(8994..9209)
                     /locus_tag="WGR04_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794916.1"
                     /translation="MNTTYTLTFDQDSLKALNLLVFTLNHLKVLEMDMEGIEDGLSAV
                     IETASEKADKLSAAFYNAVYEQEEKAA"
     gene            complement(9213..9821)
                     /locus_tag="WGR04_001378"
     CDS             complement(9213..9821)
                     /locus_tag="WGR04_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HEZ0794917.1"
                     /translation="MKDNFTMNTQLIPTVSGQLDNQTQALVDAHDLHKFLGVETPFSK
                     WIQRRIEEYGFTQALDFIGVDKIVRTEAGFFGQRDKTVQGYYLSLDMAKELCMVERND
                     KGRQARRYFIEMEKQAKALPDAVLYRIDALEDAYFQAAPEMLALLRYRSMGLNLTEIG
                     KLLDMNPGAVSYRLKKLNDLGFLEYVPKPKMVVQQQSLGLEG"
     gene            complement(9953..10153)
                     /locus_tag="WGR04_001379"
     CDS             complement(9953..10153)
                     /locus_tag="WGR04_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794918.1"
                     /translation="MDSIKKAQEKYALKRVVKTVSFNSEKELDLIKFSENLDFSNWVK
                     SKIRGDMESEKFKKKAKKNIDK"
     gene            complement(10354..10539)
                     /locus_tag="WGR04_001380"
     CDS             complement(10354..10539)
                     /locus_tag="WGR04_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794919.1"
                     /translation="MPDEKTLKAKQAEYQRKYDQKRLMKTVSFHLEREKELVDFMQKE
                     IPDFSNWVKSVMKEKMK"
     gene            complement(10813..10980)
                     /locus_tag="WGR04_001381"
     CDS             complement(10813..10980)
                     /locus_tag="WGR04_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794920.1"
                     /translation="MVDEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNIDFSQW
                     VKSIIKEKIKK"
     gene            11503..11694
                     /locus_tag="WGR04_001382"
     CDS             11503..11694
                     /locus_tag="WGR04_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794921.1"
                     /translation="MNTVKEYTAVRERLLNAADYLEEVRKDRKTGNIASVEFVPPKIG
                     ARGYGKFKVRYKTLVAVDL"
     gene            11691..12106
                     /locus_tag="WGR04_001383"
                     /pseudo
     CDS             11691..12106
                     /locus_tag="WGR04_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3157 a         2536 c         3267 g         3215 t
ORIGIN      
        1 agtggattaa atttaaacca gtacagcgtt gcctcgcctt gccgtactat ctgtactgtc
       61 tgcggctttg tcgccttgtc ctgatttaaa tttaatccac tataaatttt aatccactat
      121 aaatgcagag tgggtggaaa cactcacttt atggtttgct acgctctgct caattagcaa
      181 cccgataacc caatatggat aatagggtaa ttaatccaat ctaatttgtc agcatccgtt
      241 aatttattgc aaaataaagt attgaattat gtcgggtgca aatgacgaaa tataagtttc
      301 cgtgcggacg gatcaagatt cccactttcg tgggaatgac ggtggaaaga ttgttgtttt
      361 tcccgatgaa ttcctgtgtt ttttgttttt ccggataaat tcctgtggct ttgagttttt
      421 tggatttcag cctcaatgcc gtctgaacgc cgaatcgggc ttcagacggc attgcgtcat
      481 ttgaaattca aaaccggcca gcctttttct ttggcttctt tttccagctc ggcatcgggg
      541 ttgacggcga cgggttcgtc gacgaggcgc agcagcggca ggtcgttttt ggagtcgctg
      601 taaaaatagg ttttgccgta gctttcgagc gtttcgccgc gttcggcgag ccattggttc
      661 aggcgggtga ttttaccttc tttgaggctg ggcgtgccga tgtaattgcc ggtgtagcgg
      721 ccgtcggaac cggattcgag ttgtgtgccg atgatgttgg taatgccgaa aaggcggcag
      781 acgggggtga tgatgaactc gttggtcgat gagatgacga gggtttcgtc gcctgccatt
      841 tggtggctct gcaccagcat acgctgcata ggcgagatgt gggggatgat gtattccgcc
      901 ataaattcgc ggtgaaattc cgccagctct tctttgctgt aacgggcgag cggggcgagg
      961 tggaatttga ggaatgcgtc gatgtcgagg cagccgtttt ggtagtcgcg gtagaatttt
     1021 tcgttttgcg cttcggtttc ggcagcgtca accaagcctt ttttgatgag gtattgcggc
     1081 caggcgtggt cggaatcggt gttgatgagg gtgttgtcga ggtcgaagat ggcgaggttt
     1141 ttcattgggt ttcctgttgt ttcaaaagct ggcgcaaaag cggcagggtg atgcgtttgc
     1201 ccattgtgac ggcgtagttg tccagcgtgt cgagcatcat catcaggctg tccatatcgc
     1261 gccgccagtg tttgagcagg tattcgaaaa tttcggaatc gacggttact tggcgtgccg
     1321 ccgccatact ggcgagcgcg tcgatttttt cttggtcggt taagggtttg acttcgtaaa
     1381 cgaggcagta cgccatacgc gtccgcaaat cttcgcggat gacaagctgc tggggcgtgt
     1441 attccgaacc gagcagcaaa aagcctttgc cgctgttgcg gaagcggttg aagatggaaa
     1501 aaagcagggc ttgttcttcg ttgcccagtt tttcgacttg atcgacggcg aggtattccg
     1561 cctcgaacgc ggcatcggtc agcggcatgg aggcggcatc gatataggcg gcgtttttgc
     1621 cggcttcgag cgcctgtgcg acccacgcct gcaaaagatg gcttttgccc gcgccttctt
     1681 caccccagac atagataaac tgtccgtgtt tgtgtcggag gacatagacc agttccgcgt
     1741 tttccgtgcc gaggaatttg tcgaaactcg gatagtcgtg tgcggcaaag tcgaaaataa
     1801 gctggttcac ggttcggcat tccgaggggt ggtaaacggg tttattgtac gttgttttcg
     1861 cgcgcctttc caatttgaac gatgccgtct gaaaacggct tcagacggca tcgttcaacc
     1921 gcaggcaacg ttgccgacat cgaggcgcat attgtggaac gcgttgagcg tgcttcggtg
     1981 tccgatgctg atgatgatgc tgtcgggcag tttttgcttt aaggcgcggt agagcgcggc
     2041 ttcggccggt tcgtccaagg cggcggtggc ttcgtcgagc aggacgattt tgggcttgga
     2101 aagcagggcg cggacgaagg cgacgcgttg cagttcgccc ggggagagtt tgtgttgcca
     2161 gtcgtcggtt ttatctaatt tatcaaccag ataacccaag cggcaggtgt tcatggcttc
     2221 gattagctcc ggatgctgtt tgtcgatgtc gggataacaa accgcgtcgc gcaggctgcc
     2281 ctgtgccgtg tacgggcgtt gcggcaggaa gaggatgtct tgatgcggcg gacggctgac
     2341 tttgccgctg ctgccgaacg gccaaagccc agccagcgcg cgcagcaacg aagttttgcc
     2401 gcaaccgctc gggccgcgta tcagcaggga atcgccgctt ttgagtttga tgttgatgcc
     2461 gctcaacagg atttcgccgt tgtggcggaa cagagcgacg ttttcctgtg ggggactgtt
     2521 agtttttgca caaggaacaa atagagtaaa aaacgctgaa atctcggaaa gacgtggatt
     2581 tcggcgtttt tttgtatccg gaaaagttac gccagctttt tcacaaaacc gcgccggaat
     2641 gcgcggtttt ctgtttaaag ctgacgagat tagggaattt ttaaaactgt tttaagaggt
     2701 ttttaaaatg gatttaatca atactccggc cataccactc aacacggcct atgatggcga
     2761 tgtcgtcttg ggcattgcta aaatctattt caaacggtgc gtaacgtgga ttttcagacg
     2821 ttacaagcag tttgcccggt atgcgttgca cacgtttgac aaagaggtca ttgcctatac
     2881 gcaagacata taggccgtca cgcgggtcgg ttttggcgtg attgatgaga atggaatccc
     2941 catgattgag cacgccctcc attgaatcgc ctttaacggt aattacagac agtttttccg
     3001 gctgtttggt cacatagttg tcaatccaat atttccggaa agccaagcag aacaaaggct
     3061 cttcgccgaa gaccggtgcg ccataccctg ctgctgcggc tacgttgtag cgcggcacaa
     3121 atacaaactc tgacaagtcg acagggttgc ccatagtgtc agtaattcca tcagaatttc
     3181 tgcttacaga gaatgctcca gcgttttcca gcctggcttt atcaagatac ggtaagcctt
     3241 tgccggtcag cagccagttt aaatctcagc ctgtagattc ttgcaagaca aaggaaaata
     3301 caaaggaaga ggtttagaag ccttcctttg tatttttcat ttcaaaatca aaatttaaaa
     3361 aaaatttctc tatttaggcg actgttcact aaggaaggaa cttaaaagct gagttttcct
     3421 tagtgctgtt tattttttag cataaacatc cattagaaag tcaatcaagt gattcaaggt
     3481 gcgactatcc atttggcgag ccaagtgtag tagtacttct tctctcggag aagaaacaga
     3541 atcaatgaaa tcttggaatt tggcagcctt atttccagtt agcagccaat gcaaattaat
     3601 tccaaagtgc ttataaaact gaactaagta atcggcattc ggacttcttt ccccattctc
     3661 ataattttga atacttgccc tactgatgcc aaataactca ctcaactgct ctcttgagta
     3721 tccattatct gttctgattt tttttagtcg ctcacccaat aacttagagc ttaattcaaa
     3781 agaattatcc ataaaagttc cttttttctt gacagctaca tttgtagcta ataatattgc
     3841 tacaaatgta gcaaaacagc tagttttgta gtaattatac aggatttgaa caaaccaaga
     3901 aaggtaaaaa aatgggaatt aaaacagtaa atgaagttag agaatggtta aaagacaata
     3961 atttaacagt tgcgcaatgg gcgagagaaa acggacacaa ctccgtagat gttactcgtg
     4021 tgttgacggg gaaatcaaag tgtcgatacg gtcaggggcg cgctatcgcg attaagctgg
     4081 ggttaagaat tgacgagtga ggaaacgaag atggggataa cgattggtga gctgcttaaa
     4141 cttaatattc aagggcttcc tacaacagca agaggattaa gggagagagc aaaaacccag
     4201 aactggccgt acatcgaaga ggttggcaaa gcgcgtggag gccgtctgaa aaaatactta
     4261 atcgcttccc tccctgccga aatccgagca gccatcatga aacggcagtc ggacgagctg
     4321 acggagaaga tgccgaaaac cttgccccaa gtcagaccgg ggacggcgat gtcggctcag
     4381 gcactggctg aagcggccaa gctgttgaat gagaaacaac ggtcggtggc ggatgcgcga
     4441 tgtgcggtgg tggcggcggt gttggggatt aaatatcaat acggttgctc tgccaaggct
     4501 gcggtggctc agtttttggg cttgctggca gaaggtaaat tggacgcggt cacgcttggg
     4561 aacttggaaa aggccaatga ccgcagccgg tcggcgaagg ttggcgaacg tactttagac
     4621 ggctggatat ctgcttattt gaaagcggaa aacgcgacgg agcggttggt tgctttagct
     4681 ccgaagacga cgaaggtggt taagccgatt gagagctacg gttggttgcc gatgtttatg
     4741 cagtttcaca atatcccgtc cgcgccgaaa ctggcacaca gctaccgccg gtttgtgcag
     4801 tgggcggaag cggaaaatat gccggtcaat gatgtgccta gcttgagtat ggtgcggcgc
     4861 gtttggggca agctgccgct gattatgcag gagcgcggca ggaaaacggg ggcggcttat
     4921 aaatcgctgc tgccttatgt gaaacgtgat tggggggctt tgaagccgaa cgatgtttgg
     4981 atcggcgacg gccacagctt taaggcgaag gtggcgcatc cggtacacgg caggccgttt
     5041 aagccggaag tgacggtgat tattgatggt tgtacgcggt ttgtggtcgg tttttcggtg
     5101 tcgttggctg aaagttgtgt ggcggtatcg gatgctctgc gtatcggggt caagcatttt
     5161 ggtttgccga ttatctatta ctcggataac ggcggcggcc aaaccggcaa gacgattgac
     5221 catgaaatca cgggtattac gtcccgactg ggtatccgcc atgaaacggg tattgcgggc
     5281 aacccgcaag gtcgaggcat catcgaacga tggtggaaag acaatctgat tgagatggcg
     5341 cgacagtatg agacgtttgc gagcgcgggg atggacagca gcacgaagaa cctgatgtac
     5401 cgcaagatgg aaagtgcttt taatgctttg gaaaaaggca aggatttgac ggaggaacaa
     5461 cagaaatatt tgaaaaaact gccgagctgg tcgcgcttta tcgcggatgt ggtcaagtgt
     5521 atcgatgaat acaacaaccg cccgcacggc gagctgcccc gacatcctga cggcgggcat
     5581 tattcgccga aggcttatcg ggaaatgagg ctggaacagg acggtatcgc gccggatatg
     5641 ttgtcggcag aagagctggc gacgatgttt atgccgcaag aggtgcgaaa ggtacagcgc
     5701 ggttggctgg atttgttcaa caactcttat ttttcagtcg agctggcgga gtatcacaaa
     5761 gacgaggtac gggtcagcta cgatttgagc gatgcgtcgg tggtcaatgt gtttgatatg
     5821 gacggcaagt ttatcactaa ggcgcaggcc aacggcaata cccgcgaggc tttcccgacg
     5881 gctcgtatcg accaactggc ggaaaaccgc cgaaaaggca aaatcaagcg ggcggaaaat
     5941 gcaatcaagc tcgcaaatgc ggaagtcaat ccggctttgg aacaggcggc agtttgggac
     6001 gagctgggac atttgggcgg aaacgtcatc gaggcggagt atgcggtatt gccgagaacg
     6061 ggcacggacg attttgtgct atttgaggcg gatagaagtt aaaacggttt taaacctctt
     6121 ttaaaaggac taaaaaaatg aaacaaatta atcaagcatt gcaacaaaaa ctagctgaat
     6181 ttaaagccaa atcaggtatg aaccaaaccc aactggcacg cggtatcggt acttcgccgg
     6241 catccatcag tatgtatctg aacggcactt atgccgataa aggcggtaat tatgaaacca
     6301 tcgagccgaa aatcgaggcg tttttggaga tgcaggacag taaagcgcaa cgtgaagagc
     6361 tggtgttggg atttgtatcg actaagacga cacgccgtat tgcagaagtg atgcgcgatg
     6421 cgcacgaagg cggcgaaacg gtggtgatct acggtcaggc tggcttgggc aagacgcagg
     6481 cggtcaaaaa ctactgcgag aaaaaccctg cggccatcct gattgaggct aatccgagct
     6541 ttacggcttt ggtcttgatg cgcaagttgg cgacagcggc gaaggtatcg gcgatgggca
     6601 gcctgaatga tttgtttgag tctgtatctg accgcctgcg cgattcgggc cgtctgattg
     6661 tggtcgatga agcggaaaac ctgccgttac gcgcccttga aattgtacgc cgtctgcacg
     6721 atgagactgg ctgcggcttg gtgttgagcg gtatgccccg actggttgcg aatttgcgcg
     6781 gtaagcatgg cgagctggtg caactttaca gccgcgtgtc ggttgcgctg aatttgggcg
     6841 aatctttgcc ggatgacgaa ctctttgaga ttgcgaaagc ggctttgcct gatgcggacg
     6901 aagaaacgct cttggaactg gttaaacata gcaacggcaa tacgcgccgg atgagcaaat
     6961 tgatgcgcgg cgcggtacgc acggcgaaca agaacggtat caagatgcag gtcggtatcg
     7021 ttaagaaata cagctccctg attatccgat agggccgtct gaaacggtaa gtctctgaca
     7081 gggctatata ttttttaccc tatgatttta ataacttatt gttttctaaa ggaaaacgca
     7141 aaatgcaagt tttgaagaaa gttgattgga agatgtttgt ggcacgctct ttttggcggt
     7201 gtgtgccggt tggtttgacg gcgggcgtgt ggtgttttgt ggcggggatg gcgttgcatt
     7261 cctgcgcgaa agaacccgaa ccggttgcga aagagctgac gaaggtcgag aagatggaaa
     7321 gacaggcgga tttggaggtc aaggtatggg aagagcaata cgagtcaatg agcgtcgagg
     7381 aaaaaatgaa agggattgtt tatgagtgat aagccaattt tattgagccc tgcggcgaaa
     7441 aaagaggctt tggatcgggc ggtaaaggaa atccgtgcaa aatatggcga taaggcgatt
     7501 gtgaaaggat gtgtgaaatg agtttcggac gacgtaatac ggattggcag gcttggggac
     7561 aacaccgcag gcgtgcgacg gcgcgaatgg cgcaaaaaag ccgggagcgc gaaatcgagg
     7621 aatatcaggc gcagtttgaa gataaggacg gcaaaggtcg tctgaaaacg ggaggaaaca
     7681 aagatgaatg aaaaagattt gatcgaatgg ctcgaagacc gtggcgagct gatggtcatg
     7741 aagaaggacg gcgaaggctt tgtgattgca gcgcgtgcgc cggacgggat gtggaagact
     7801 gccgaggcgg aaactttggc tcaggcgata actttatggg aggaagtgtg atgtttaagt
     7861 ttttgagaaa ccgcgcgaaa gttgaagcaa ttttaaacaa gaaaggaaag tgaaatgaaa
     7921 gtgtatgttt ttaaaattag taatgaaaac ggtaagttaa agatagaact ccccgaaatc
     7981 ccaatgggta agcaaattga cgaagttgat ttgattgctg ggctaaccac ggaatttatt
     8041 gcaagtatgt tacgtgatgc ccaaaaagat cgtcgcaaat tcgtaatcga cgcatcaaat
     8101 caattggctg caatccagac atatcaaaaa atcttcaatt aaggaaggaa gagaaaatgg
     8161 ctaaaacccg aatcaaacag cccgccatcg aagcggcaca agacaaaacg gaagttactg
     8221 catttatccg ccaaatcggc gacttgcagc gcgaagtcaa acgcctggaa accgaagccg
     8281 gagacaaaaa agcggtcatc gaagaagaat atgccgccaa agccgcgccg atgtgtgccg
     8341 aaatcatgag cctgaccgaa cgtgtggccg catactgcga ggcacataag gacgagctga
     8401 cggaaaacgg taaaaccaaa accgtggact ttactacagg cctgattaaa tggcgcatcc
     8461 gtccgccatc cgtcaaggta acgggcgtgg ccgccgtctt ggcgtggctc tcggagaaat
     8521 ccgccttttc cgagtttgtc cgcacaaaaa aggaaatcga caaagatgcc atcctgaatc
     8581 aaaaagagcg tttttcagac ggtcaagtgc cgggaattaa gattgtgtcg gggcttgagg
     8641 attttgtgat tgagccgact gagcaggagt tggcgtgatg gaaaacggaa atttaaatac
     8701 cgatgaactg gaagttttaa gaactgccgc acgcgattcc ttctacatcc acgctcaagt
     8761 cgaaaatgcc aaccgaaaat tggaaaccgc ttttttcgtg tgggaaaaag tgaaagaagg
     8821 agaaaaagag gctatgcgcg cccgaaaaaa agcctttatt tattactgct tcggggtggt
     8881 ttggtttttt cttgcgttga ttttattttt ctttgtcgtt taaagctgga ttaaaggccg
     8941 tctgaaacgg ggtttaaaac ctgtttcaga cggccttttt tcatgcccta gattcaggct
     9001 gctttctctt cctgctcgta cacggcgttg tagaaagcgg cggacagctt gtcggctttc
     9061 tcggaggcgg tttcaatcac ggcagacaaa ccgtcttcga ttccttccat gtccatctcc
     9121 aaaactttca gatggttgag cgtgaacacc agcagattca gggctttgag gctgtcttgg
     9181 tcgaaagtca gagtataggt ggtattcatg gcttaaccct ccaatcccaa agactgttgt
     9241 tgcaccacca ttttgggctt ggggacatat tccaagaaac ccaaatcgtt gagttttttg
     9301 agacggtagg aaaccgcgcc ggggttcatg tccaagagtt tgcctatttc ggtcaggttc
     9361 aagcccatac tgcggtagcg cagcagcgcg agcatttcgg gcgcggcttg gaaataggcg
     9421 tcttccaatg catcgatgcg gtatagcacg gcatcaggca gggcttttgc ctgtttctcc
     9481 atttcgataa aatatcggcg ggcttggcgt cctttgtcgt tgcgctccac catgcacagc
     9541 tcttttgcca tgtcgaggga taaataatac ccctgaacag ttttatcacg ctgtccaaag
     9601 aaacctgctt cggtgcggac aattttgtcc acgccgataa aatcaagggc ttgtgtaaat
     9661 ccgtattctt caatgcgacg ctgaatccat ttagaaaatg gtgtttccac acctaagaac
     9721 ttatgcaggt catgagcatc aaccagtgct tgtgtttgat tgtccaattg acctgaaaca
     9781 gttgggatga gttgggtatt catggtaaaa ttatctttca tttcaaggtt gagatgacaa
     9841 ggaaaagaac gctctaacgt tgcttttcca ctatccccga taagctgtca cttatcgggg
     9901 atttccatat cttgtgttgc acaagatatg gaaattttaa attatggtaa ttctatttgt
     9961 caatattctt ttttgctttc tttttgaatt tttccgattc catatctcct ctaattttcg
    10021 attttaccca gtttgaaaaa tctaaatttt cagaaaattt aattaaatca agttcttttt
    10081 cggaattgaa cgatactgtt ttcactactc gcttgagtgc gtatttttct tgagcctttt
    10141 taatcgagtc cataatcttc ctttaaatag gttttaaaac acgattatgg taatactaaa
    10201 aaccatttta atcaagatgt attacaacaa aaagccgtct gaaatggggt ttaaaacctg
    10261 tttcagacgg ccttttttca tgccctacat aaagagagtt gctgctctgc tttccacctg
    10321 ttccgccgat gttttcccat cggcggaatt ttcttatttc attttttcct tcatgactga
    10381 tttaacccag tttgaaaagt cgggaatttc tttttgcata aaatccacca actctttctc
    10441 tctttccaaa tggaaagaaa ctgttttcat cagccgcttt tggtcatact ttcgctgata
    10501 ttcagcttgt tttgctttta gcgttttttc atcaggcata ggcaatcctt gatttttaaa
    10561 atcgttgtag taaaatggct tgcagaaagg ttggcgaaag gggcgcggtg ttctgccacc
    10621 ccttgtgttt aatcaacttg cttagtaagc cggactacag cttaaaagca gaatgatgaa
    10681 aaaaaacaac tttcgccagt tttgtcattt ccacaagcct cctttctatc cgataagccc
    10741 ccgaagttgc cgcttcgggg ctttgcttta tcagataggt gtattatcta gtatttctag
    10801 aatttagtca agctattttt tgattttttc tttgatgatt gatttaaccc attgggaaaa
    10861 atcgatattt tgggcatatt ccaaaagttc tttttccgtt tcggcattga acgagacttt
    10921 cttaatcacc cgtttctgct cgtaacgctt tcggttttct gccagttttt catcaaccat
    10981 aggcaaactc cttgattttt taaaccgtat tttgtaagat tgggactggg gacagcggcg
    11041 accgccccca gctggtcagt ctttagtagg cattagcact tgccaacatg aaaactgcga
    11101 ccaagatgag aatttgaata gtgcgtttat tcatcttctt tctcccagtt gagccccgct
    11161 tcggtggggc ttttcccgta tcggccctca ctacgccgat gaattgaatt ataaacgtgg
    11221 acacgttaaa agtcaaccct ttccccaaca aaatcaaaga aaaatgccgt ctgaaaacag
    11281 ctcgaatgct gtttcagacg gcattttttg ttgtcgggca tcaatgccct gccatttgtg
    11341 cggcgttgcg gttatcaaga tatggtattt tagtgtgtat ttttagaaga aatttaccat
    11401 atatagtatt aaaatattat acggttgttt tatatggatt tgattttctt gaaaaataaa
    11461 tcccatatgc taatatttgc actatttggg cgaggttgaa aaatgaatac cgtcaaagaa
    11521 tatacggcag tccgcgaacg gctgctcaat gctgccgatt atttggaaga agtccgtaaa
    11581 gaccgtaaga cgggcaatat tgcgtccgtg gaatttgtgc cgccgaaaat aggggctcgg
    11641 ggttacggca aattcaaagt ccgttacaaa accttagtgg cggttgattt atgagcgggg
    11701 aaaaatccga gaatctgccg gccgatgagc agcagaaaat gcttcaggag tttttagaac
    11761 ttcaaaaaca agatattgca gtcaaacaga aggagttgga aagccgtaag gacgaaatcc
    11821 aatctcacga acgcattgca ctggctacca tcgaagcgca aaaacagggt gaacagcaac
    11881 atggtgagat tttaaagagg tacacaaacg ccgtctgaat gccgtgattg ctatttcttt
    11941 tctgattgcc gccgtgctga ttactacgtt atggcgcgat aaggacagcg tcgctattga
    12001 aattgtcaaa atcggcgggg cggtagcttt gggatatttt gcaggaatca acagaggtaa
    12061 agcgcaaacc ttggaaaagc aacggcgcga aaaagacagt gattagattg tagtaaaaag
    12121 gtcgtctgaa acgttttggc gaaactcgca aagctcgttt tcagacggcc ttttt
//
LOCUS       DAWFJR010000055        12086 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWFJR010000055 DAWFJR010000000
VERSION     DAWFJR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12086
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.055"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(242..3094)
                     /gene="gcvP"
                     /locus_tag="WGR04_001384"
     CDS             complement(242..3094)
                     /gene="gcvP"
                     /locus_tag="WGR04_001384"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ0794922.1"
                     /translation="MKLSELFNPDEFAARHLSFGDEAALLAAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPDALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDERVYPQTLDVMKTRAKYFGFELVVSDFAQADEGEYFGALFQYVGKDG
                     DVQDLQSVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFTDALVSDGLNVVHKVFFDTVTVDFGSK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSAYENLVDLYRAFTGKDTFTFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLADMENSLKAITGFDAISLQPNSGAQGE
                     YSGMLSIRRYQETNGEGHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIVHENGGQVYMDGANLNAQIGIM
                     QPAKVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHALTDTHSTSAGQT
                     AVAAAAYGSASILPITWMYIAMMGKQGMEQATRWALLNANYVAKRLGEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVGRGEWPKDDNPLVNAPHTAADITGNWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3125..3483)
                     /locus_tag="WGR04_001385"
                     /pseudo
     CDS             complement(3125..3483)
                     /locus_tag="WGR04_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3431..3661
                     /locus_tag="WGR04_001386"
     CDS             3431..3661
                     /locus_tag="WGR04_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0794923.1"
                     /translation="MISACPGFIHSRFGRSVMPSEPADRLFQKTPYRCNFMRLYGVGF
                     ALQTAYASRSSLVSQSVHDYINNLGADKPLFI"
     gene            complement(3654..4295)
                     /locus_tag="WGR04_001387"
     CDS             complement(3654..4295)
                     /locus_tag="WGR04_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ0794924.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4285..4836)
                     /locus_tag="WGR04_001388"
     CDS             complement(4285..4836)
                     /locus_tag="WGR04_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ0794925.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIDSDNGLFERLVLEI
                     NQAGLNWTLMLKKQQAFQTAFEGFDIDTVAAFDDTDRERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDTHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            complement(4840..5358)
                     /locus_tag="WGR04_001389"
     CDS             complement(4840..5358)
                     /locus_tag="WGR04_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ0794926.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDNTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5358..7019)
                     /gene="pqiB"
                     /locus_tag="WGR04_001390"
     CDS             complement(5358..7019)
                     /gene="pqiB"
                     /locus_tag="WGR04_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ0794927.1"
                     /translation="MTDNSPPPNGHAQARVRKNNILLSAVWLVPLIALIAGGWLWIKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQIESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQLQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATQGGGLDDLQAKVVDLLDKFDKLPLDKTVAELN
                     GSLAELKSALKSANAALSSIDKLAGNPQTQNIPNELNQTLKELRTTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(7012..8472)
                     /locus_tag="WGR04_001391"
     CDS             complement(7012..8472)
                     /locus_tag="WGR04_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ0794928.1"
                     /translation="MGSAVGGQPLEKIFLPDTKGLFILPNLPVLHPDWVYRLFSFIMP
                     PLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRADVPRLDRGEAAFCPR
                     CGYKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPGAASVLSLPEMMRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKRVYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGDN
                     AVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLSISSAFLTAAVILYFPA
                     NILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIA
                     SARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKHD"
     gene            8810..9439
                     /locus_tag="WGR04_001392"
     CDS             8810..9439
                     /locus_tag="WGR04_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ0794929.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLADLGDKPYEYGKPLPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPNGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            9614..11983
                     /gene="hmbR"
                     /locus_tag="WGR04_001393"
     CDS             9614..11983
                     /gene="hmbR"
                     /locus_tag="WGR04_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent hemoglobin receptor HmbR"
                     /protein_id="HEZ0794930.1"
                     /translation="MKPLQMLPIAALVGSIFGNPVFAADEAATETAPVKAEIKAVRVK
                     GQRNAPAAVERVNLNRIKQEMIRDNKDLVRYSTDVGLSDSGRHQKGFAVRGVEGNRVG
                     VSIDGVNLPDSEENSLYARYGNFNSSRLSIDPELVRNIEIVKGADSFNTGSGALGGGV
                     NYQTLQGRDLLLDDRQFGVMMKNGYSTRNREWTNTLGFGVSNDRVDAALLYSQRRGHE
                     TESAGNRGYAVEGEGSGANIRGSARGIPDPSKHKYHSFLGKIAYQINDNHRIGASLNG
                     QQGHNYTVEESYNLLASYWREADDVNRRRNTNLFYEWTPESDRLSMVKADVDYQKTKV
                     SAVNYKGSFPIEDSSTLTRNYNQKDLDEIYNRSMDTRFKRITLRLDSHPLQLGGGGRH
                     RLSFKTFASRRDFENLNRDDYYFSGRILRTSSTIQHPVKTNNYGFSLSDQIQWNDVFS
                     SRAGIRYDYTKMTPQQLNAECHACDKTPPAANTYKGWSGFVGLAAQLNQAWRVGYDIT
                     SGYRVPNASEVYFTYNHGSGNWLPNPNLKAERSTTHTLSLQGRSEKGTLDANLYQSNY
                     RNFLSEEQKLTTSGTPGCTEENAYYGICSDPYKEKLDWQMKNIDKARIRGIELTGRLN
                     VDKVASFVPEGWKLFGSLGYAKSKLSGDNSLLSTQPLKVIAGIDYESPSEKWGVFSRL
                     TYLGAKKVKDAQYTEYEGYRFIKKVKDYPWLNKSAYVFDMYGFYKPVKNLTLRAGVYN
                     LFNRKYTTWDSLRGLYSYSTTNAVDRDGKGLDRYRAPGRNYAVSLEWKF"
BASE COUNT         2850 a         3105 c         3305 g         2826 t
ORIGIN      
        1 ttgtttgtcc gtgtggaaat gacatcaaag ggtgtgaata ggcttttcat ctttgaaatc
       61 tgtttgcacg atcggatgaa taaaaagtag gatgataaaa aagctgatgt atggatactg
      121 gtttaactca tttgttttaa agttatagtc gagatacgtg tattggtgca accagtctgc
      181 cgtcgacgaa aatgccgtct gaaagtgttt cagacggcat ttgtcttcgg ggtttcaacg
      241 gtcagtcttc ataattttcc atcggtgggc agctgcacac gagattgcgg tcgccgtaca
      301 cgtcgtccac gcggttgacg aagggccaaa acttgtgttc gcggacgaac ggcaacggga
      361 agacggcttc ttcgcgggaa tacggatgcg cccagttgcc ggttatatct gcggcggtgt
      421 gcggcgcgtt gaccagtggg ttgtcgtctt tcggccattc gccgcgcccg actttcagca
      481 cttcctgttt gatttgtttc agggcggcga tgaagcggtc gagttcggct ttgctttcgc
      541 tctcggtcgg ctcgatcatc agcgtaccgg caacggggaa ggaaaccgtc ggggcgtgga
      601 agccgtagtc catcaggcgt ttggcgatgt cggtttcggt aatgccgctt tcggctttga
      661 gcggacgcaa gtcgacgata cattcgtgcg cgacgcgtcc gtttttgcct gtgtacagaa
      721 tcggatagtc ttcgcccaga cgtttggcga cgtagttggc gttgagcaat gcccagcgcg
      781 ttgcctgttc catgccttgt ttgcccatca tggcgatata catccaggta atcggcagga
      841 tggatgcaga accatatgct gcggcagcca cggcggtttg cccggcactt gtgctgtgcg
      901 tgtcggtcaa agcgtgtccc ggtgcaaacg gggcaaggtg ggctttcaag ccaatcggac
      961 ccatgcccgg gccgccgccg ccgtggggga tgcagaaggt tttgtgcagg ttcatgtgca
     1021 acacgtctgc gccgactttg gcaggctgca tgatgccgat ttgggcgttg aggttggcgc
     1081 cgtccatgta aacctgtccg ccgttttcgt ggacgatgcg gcagatgtcg cggatgcctt
     1141 cttcgtacac gccgtgggtg gacggatagg taatcatgat ggcggacaaa gcgtcgcggt
     1201 gttgctcggc tttggctttc aaatcgtcga tgttgacgtt gccgtgttcg tcggtatcga
     1261 caacgacgac tttcaagccc agcatggccg cagtcgcagg gttggtaccg tgggcggatt
     1321 tgggaatcag gcagatgttg cggtgtccct cgccgttggt ttcttggtaa cggcggatgg
     1381 acagcatgcc gctgtattcg ccttgtgcgc cggagtttgg ttgcagggaa atcgcatcaa
     1441 aaccggtgat ggctttcagg ctgttttcca tatcggcgag caattcgcgg tagccggcgg
     1501 tttgcgcttc gggagcgtaa gggtggatgt cggagaactc agcccaagta atcggcaaca
     1561 tttccgcagt cgcgttgagt ttcatggtgc agctgcccaa tgaaatcatg ctgcggttca
     1621 tcgccaagtc gcggtcttcg agttttttca ggtagcgcag catttcgtgt tcggtgtggt
     1681 aacggttgaa cacaggatgt tgcagaatgt cgtcttgacg cagtaattcg gcattcagac
     1741 ggcctttgac atcatcggca aatgtgaacg tatctttgcc ggtaaatgcg cggtacaaat
     1801 cgaccaaatt ttcgtatgcc gacgtttcat ggaatgcagc cgcaacttga gtatcgttaa
     1861 cgcggcgcag gttgtaaccc gattccaaag cagcagcaaa cacttggtct gctttctctt
     1921 tgctgccgaa atcgacggta acggtatcga agaagacttt gtgaaccaca ttcaggccgt
     1981 ctgaaaccag cgcatcggta aaggcagaag ccaacgcatg aatgcggttg gcaatgcgtt
     2041 tcacaccttc agggccgtgg taaacggcat acataccggc caagttcgcc agcaatgcct
     2101 gtgccgtaca aatattggat gtcgcttttt cgcggcggat gtgttgctca cgggtggaca
     2161 acgccatgcg caaggcaggt ttgcccgatg cgtctttgga tacgccgatg atgcggcccg
     2221 gggcggagcg tttgaactcg tctttaaacg cgaaataagc ggcgtgcggc ccgccgaagc
     2281 ccatcggtac gccgaagcgt tgggtgttgc ccaaagcaat atctgcaccc aattcagccg
     2341 gagacttcag caaaaccaag ctcatgatat cggcggcaac ggcaacaatc gtgcctttgg
     2401 ctttcagacg gcctatgacg ctttgcaggt cttgcacatc gccgtctttg ccgacgtatt
     2461 ggaacagcgc gccgaagtat tcgccttcat ccgcttgggc aaaatcgctg accaccagct
     2521 cgaagccgaa atatttggcg cgggttttca tcacgtccaa agtttgcgga tacacgcgct
     2581 cgtccacaaa gaaacgctcg gatttcacct tgcccacgcg gtgcgccatc gccattgctt
     2641 cggcggcggc agttgcctcg tccagcaaag acgcgcccgc cacgggaaaa ccggtcaaat
     2701 cgatacacac ttgttggaag ttcaacaacg cttccaaacg accctgggca atttccgcct
     2761 gatacggcgt gtaggcggta taccagcccg gattttccaa tacgttgcgc aaaatcacat
     2821 tcggcacgcg ggtcgggtaa tagcctaaac cgatatagga cttgttgatc acgtttttcg
     2881 acgcaatgcc tttcaatttt gccaacgcgt ccgcttcggt cagggcatcg ggcaaatcga
     2941 gttcagacgg catacggatg ctttgcggca cggtgttgcc gacaaaatcg tccatacttt
     3001 tctcgccgac agcggcaagc aacgccgctt cgtcgccgaa actcaaatgc cgcgcggcaa
     3061 attcgtcggg gttgaacagt tcggacagtt tcatttctac tccttcgggt ttgtagggct
     3121 gccatcaggc agaattttta gacatcggat gattcgggca agggtagcac aaaagacggg
     3181 ataatggcgg acaacgcgcc agaatgcagc aggtcaggac aagcagcgcc acaagcggca
     3241 gcgataaggg aaagccgccg accaaaacca tgaaacctgc aggtaccagc caagccaatg
     3301 gatacgccgg aagcggaaaa caatattgag cagaaccaaa atcacgacaa acagcggcag
     3361 cctcgccatc aggttggcac gcctgtatgt tgcgtacggg tcggacgctt cttcaggttc
     3421 gcaatcttcc atgatttcag cctgtccggg ctttatccac agcaggttcg gacggtcggt
     3481 catgccgtct gaaccggcag accgtctttt tcaaaaaacg ccataccgct gcaacttcat
     3541 gcggctgtat ggcgtaggtt ttgcacttca gacggcatat gcgtcccgaa gcagtttggt
     3601 gtcccagtcg gttcatgatt atataaacaa cttgggtgcg gataaacctt tgtttatcta
     3661 aacccctgtt ttctcaaaaa ttgattcaaa tcttcggcaa atttccgata gcctttgccg
     3721 ttggcgtgga tttggtcgga tttcagatta ttatcgccca aaatttccgc ccacgcgccg
     3781 ccgaacagcg gaatgccgta ttcctcggac aaatcctcat acagcggatg atcgctcaaa
     3841 tgcccgaaca acgcgcccag tgtgatgtgc ggcacgccga cgaggacggc ggggatgttt
     3901 tccttctgca cggtttcgat gattttcgcg atattggcgc gggtctgctc cttgggaact
     3961 ttgcgcagaa agtcgttgcc gcctatgccg acaatcacaa gcttgggttt gcgtgccaac
     4021 agcgcgggca ggcgcgacag ggcttgggcg gatgtatcgc ccgatacgcc gccgttgaca
     4081 atattccaac ccgtcagttt ttgcagttgc gcggggtagg attcgccagg gtttgcgccg
     4141 tagccgaagg taagcgaatc gcccaaggca agtacggtgc ttccttcggg aattttggcg
     4201 tgggttcggg cggattttct gccgcaggcg gtaaggagca acgcgcctgc gccgaggagg
     4261 aaggttcttc tgttcattgg tttttcagat ggcatcgagc catttcgggt ggtatttcag
     4321 ggtttcacgg taaaccggac agctttcggc gtgcgcgcct ttgaggtagc cggtactcat
     4381 cagaaattcg ccgacgattt cgccgccgac gaatttgaaa tgttttttaa agagtttaac
     4441 ccattcgtct ttgcttcgcg gatggtgcgt gtcgagccag ttcttgaacg agccgtattc
     4501 ttgttgcaac gcttggattt gccgtgcatt gaaaatggcg gcatcgattt tcaggcggtt
     4561 gcggacaatg cccgcgtcgg caagcaggcg ttcgcggtcg gtgtcgtcga aggcggcaac
     4621 cgtatcgatg tcgaaacctt caaatgccgt ctgaaatgcc tgctgcttct tcagcatcag
     4681 cgtccagttt aatcctgcct gattgatttc caacaccagc cgttcaaaca gtccgttgtc
     4741 gctgtcaatc ggaaaaccgt attgcgtgtc gtggtaatgt ttgttcgggt tatcggtgtt
     4801 ttcgggaagt gaggcggcaa attcgcaata gttcacgttt tactcgacca tctgttgcgc
     4861 cgcctgtttc agtccctgtt cgagtgcggc ggtcatcgcg gcgtagccgt caccctgctg
     4921 ttcggtttcg atatggaagg gtctgttcgt accgtcgggt aggacggcgt agccgctgat
     4981 gagggttttg cccgtgtagc tgccttggaa tgcgtcgata tagaccgtcc atttttcggt
     5041 actgccgctg cgtgaggcag gaacaaagat gcgtgtgttg tccaaacggt tgaatgcatt
     5101 gctcaacgcc gcttcgagca tatcgtccaa ggtgtctgcc caaacgtggt tttgtgcggt
     5161 gttgaggcgg taggggtcgg tttgatagac cagtccgccg cgtttgagcg gttcggcaag
     5221 acggacttcg acggcggttt cgccgccttg tgttgcagga cggatgtagc ggctgtcggg
     5281 caacacgaaa tattgtgtgc tttgcacagt accgcaggcg gcaagcgtca gggcggcggc
     5341 aatcgggaag aggcgcatta tcggcttcct ttcgggatgg ggtctttgct gctgctgttg
     5401 aaaatcagcg cgttgggttt ttctttcaaa gtattaatta cgggttgaac gtcttttaag
     5461 gttttgtcca aactttgcaa cgtattttgt acgtcgccgt agataggcga ttgaggcgat
     5521 acgccttgca gggttgtgcg caactctttc agggtttggt tcaactcgtt cggaatgttt
     5581 tgcgtctgcg gattgccggc cagtttgtca atggagctta gggcggcatt ggcagatttg
     5641 agtgcggact tgagctcggc aagcgaaccg ttcaattcgg caaccgtctt atccaaaggc
     5701 agtttgtcga acttgtccag caaatccacc actttcgcct gcaaatcgtc caaaccgccg
     5761 ccttgtgtag cgatgacggt atcgcctgca taaacggtat gcggtcgcag cttgggcgaa
     5821 gacgaaggct gatcgttcaa ctcaatcatt ttgctgccgg tcagcaggtt gttgctggag
     5881 atggtggcgg tcaggccttt gtttaaagcc gtctgcaatt gttgtttcca atgctctttg
     5941 ctttgttcgt cggcattgat ttccaaacgg gaaggctcaa tgcggatgcg cacgggaatc
     6001 cagccgtttt caaacaggtg caggctgtcg ttgcggtcaa aataagggac atcggaaacc
     6061 acgccgacat tcagcccttt gtattcaaca ggcgaaccga cggtcagtcc gcgcacggat
     6121 tgtttgaaaa acgcggtgta gtacagcgag cggtcgtcag gcaggttggc gacttcgctg
     6181 cggctgtcgt aaagcgtgaa gctgtcttcg cttttgacgt ttttactgtt tttggttttc
     6241 ggcgaatcaa atgaaatcgc gcccgacagc agggcaggca gaggggcgga attgagtttg
     6301 atgccgctgc ctgtggtttc gatattaatg ccgctttcca gccaaaaacg gctggcggaa
     6361 tgaatcagtt tgtcgttggg gctttggatg aagatggtgt aatgcacgct ttggtcggac
     6421 gggtcgaaat gcgcgctttc gatttgcccg accataaagt tttcatacaa aacagggctg
     6481 ttgacgttga ggatacggtc gtttttacca atcaaattca agcgcagacc gctttgcccg
     6541 atggcggtaa cgggcggaat gtcctgcact tggaacacgt cttttgcctc gtcgcttttg
     6601 ccgggtgtaa aggcgatgta cgaacccgaa agcagcgtac ccaaaccggt tacgccgctt
     6661 tggtcgatac gcggcttgac cacccaaaac tgtgtgtcgc tgcggatgag gccggatacg
     6721 tccgcattga gttgggcggt tacttccacg cctttctggt cgtcgcgcag tttgattcgg
     6781 gtaacgcgtc cgacatcgat gctcaatacc ttaatgaccg tattgttgac ctcaatgcct
     6841 tccgcgctgt ccatcaagag cgtaaccaca ggccccctgt tgcggatttc cttaatccac
     6901 agccagccac cggcaatcag cgcaatcagc gggaccagcc agacggcgga gagcaagatg
     6961 ttgtttttgc ggacgcgtgc ttgggcgtgt ccgtttggag gagggctgtt gtcagtcatg
     7021 tttttccgtt tcgttgaaag caatgccgtc tgaagcgcgt ttgtcccaaa gcaggcgcgg
     7081 gtcgaaataa taggcggaca gcatcgtcag aatcacgacc aggcagaaat agactgccgc
     7141 actgcccgga atgacgcgcg cggcataagt gtggaacgaa cacatcaaaa taataatcac
     7201 aaaaatatca atcatcgacc agcggccgac cgcttcggtg atgcggtaga ggtgcgacaa
     7261 tttctttgca cccgttggca aagcgaagcg ggcggacgca atcaaaaccg acattgccgc
     7321 aatcttcagt accggcacca aaatactcgc gctgaaaata accgccgcaa tcagcctgtc
     7381 gccctcgtcc cacatataag cgatgccgtt aaggatggta ttgacctccg tggcggcagg
     7441 attggacgaa atcataatcg gcaggatatt ggcagggaaa tacaaaataa ccgccgccgt
     7501 cagaaacgcc gacgaaatac tcagactttt cggccgtcgg cggtacagtt ccgcgccgca
     7561 cacaccgcag ggggattcgg cactgtcgcg gaaatacagg cagcggctgc aacaggtttt
     7621 gccttccgat gccgtctgaa ccgcattatc ccccgtcagc cgcccgattt gaaaatacac
     7681 ccaatgctgg ggaacggata ccgaagtccg aatcagcata accgacagcg cgaacatcag
     7741 ataaaacgcc gacccgaagc gaacctctgc cacagacgag agcttgatat acgccaccag
     7801 cgtggaaaca aaaaacacat ccaccatcat cgcctgcctc aagcgcacca tcacacgcgt
     7861 tgccaaacgc agcgcaggat acacccgttt ccgtatcagc gcggcataga catacaggca
     7921 cagcagcaga aacagaaccg gcgcgccgaa ggtcagcaca aacatcactt cggccaaaaa
     7981 accataatcc tgaaacacca tcaggcgcat catctcgggc agcgaaagga cggatgccgc
     8041 acccggtatc ccgacctcga tatacgtcat accgtaagca aacgccatca aaatcagcga
     8101 agccgccgca taagcgggcg gggcggaaaa aggatgcctg cccaccctga agagcttata
     8161 gccgcagcgc gggcagaacg ccgcctcccc cctgtccaac cgaggcacat ccgcgcggca
     8221 gccgcattcc gggcaatcta ccgtatgcgg cggtagggct tcgtcgcggc agttgccgcg
     8281 aaggctgtgg gggatatttt cggcaaacgg cttcatcggg aaagtggcaa atataagtgg
     8341 cggcattata aaggaaaaaa ggcgatacac ccaatcagga tgcaaaaccg gcaggttcgg
     8401 cagtatgaac aaacccttgg tgtccggcaa aaatattttt tcgagcggct gtccgccgac
     8461 tgccgaaccc atttgataaa atcccgattc tgtaaagaat agtaaaatac ctgcttaaaa
     8521 acatcacgca aatgatattt tttccaaaaa tatgtgataa taaatttcgt ttatgattct
     8581 ttattatttt tatagtggat taacaaaaat caggacaagg cggcgaagcc gcagacagta
     8641 caaatagtac ggaaccgact cacttggtgc ttcagcacct tagagaatcg ttctctttga
     8701 gctaaggcga ggcaacgccg tactggtttt tgttaatcca ctatatatgc gggcaagtgc
     8761 ggattttgcc cgccccgcat ccttatccct taccgacaaa ggaaaactta tgagtgaaac
     8821 cgaaaatcaa gccctgacgt ttgccaaacg tctgaaggcg gataccacgg cggttcacga
     8881 cagcgtggat aacctcgtta tgtctgtcca accgtttgtc agcaaagaaa actacatcaa
     8941 atttttgaaa ctccaatccg ttttccacaa ggccgtcgac cacatctata aagatgccga
     9001 attaaacaaa gccattcctg agctggaata catggcgcga tacgatgccg taacgcaaga
     9061 ccttgcagat ttgggcgaca aaccttacga atacggcaaa ccgctgccgc atgaaaccgg
     9121 caacaaagca atcggctggc tttattgcgc cgaaggatcc aatttgggcg cggcgttttt
     9181 gttcaaacat gcgcaaaaac tcgattacaa cggcgaacac ggcgcgcgcc acctcgctcc
     9241 ccatccgaac ggtcgcggca aacactggcg tgcctttgtc gagcatctga acgccttgaa
     9301 cttaactccg gaagccgaag cagaagccat tcaaggtgca cgcgaagcct ttgcattcta
     9361 caaagtcgtg ttgcgcgaaa ccttcggctt ggcagccgat gccgaagccc ccgaaggtat
     9421 gatgccgcac aggcactaaa aaataatcga atcaaataaa caaggtctcg gcatagctgt
     9481 ttgcaggggc ctttaattac acggcgcggc tttgtttgca tggattactg tcttattaaa
     9541 tattaatgat tatcagaaaa tctattatgc gctaaccgac ggatgaacaa tccatacatc
     9601 ttgagttgat aatatgaaac cattacaaat gctccctatc gccgcgctgg tcggcagtat
     9661 tttcggcaat ccggtctttg cggcagatga agctgcaact gaaaccgcac ccgttaaggc
     9721 agagataaaa gcagtgcgcg ttaaaggcca gcgcaatgcg cctgcggctg tggaacgcgt
     9781 caaccttaac cgtatcaaac aagaaatgat acgcgacaac aaagacttgg tgcgctattc
     9841 caccgatgtc ggcttgagcg acagcggccg ccatcaaaaa ggctttgccg ttcgcggcgt
     9901 ggaaggcaac cgtgtcggcg tgagcataga cggtgtaaac ctgcctgatt ccgaagaaaa
     9961 ctcgctgtac gcccgttatg gcaacttcaa cagctcgcgt ttgtctatcg accccgaact
    10021 cgtgcgcaac atcgaaatcg tgaagggcgc agactctttc aataccggca gtggtgcatt
    10081 gggcggcggt gtgaattacc aaacgctgca aggccgtgat ttgctgttgg acgacaggca
    10141 attcggcgtg atgatgaaaa acggttacag cacgcgtaac cgtgaatgga caaataccct
    10201 cggtttcggt gtgagtaacg accgtgtgga tgctgctttg ttgtattcgc aacgtcgcgg
    10261 tcatgaaacc gaaagtgcgg gaaaccgagg ctatgctgtg gaaggagaag gcagtggcgc
    10321 gaatatccgt ggttcggcac gcggtatccc tgatccgtcc aaacacaaat accacagctt
    10381 cttgggtaag attgcttatc aaatcaacga caaccaccgc atcggcgcat cgctcaacgg
    10441 tcagcagggg cataattaca cggttgaaga gtcttacaac ctgcttgctt cttattggcg
    10501 tgaagctgac gatgtcaaca gacggcgtaa caccaacctc ttttacgaat ggacgccgga
    10561 atccgaccgg ttgtctatgg taaaagcgga tgtcgattat caaaaaacca aagtatctgc
    10621 ggtcaactac aaaggttcgt tcccgataga ggattcttcc accttgacac gtaactacaa
    10681 tcaaaaggac ttggatgaaa tctacaaccg cagtatggat acccgcttca aacgcattac
    10741 cctgcgtttg gacagccatc cgttgcaact cggggggggg gggcgacacc gcctgtcgtt
    10801 taaaactttc gccagccgcc gtgattttga aaacctaaac cgcgacgatt attacttcag
    10861 cggccgtatt ttacgcacgt ccagcacgat tcagcatcca gtgaaaacca acaactacgg
    10921 tttctcgctg tctgaccaaa tccaatggaa cgacgtgttc agcagccgcg caggtatccg
    10981 ttacgactac accaaaatga cgccgcagca actgaatgcc gagtgtcatg cttgtgacaa
    11041 aacaccacct gcagccaaca cttataaagg ctggagcggt tttgtcggct tggcggcgca
    11101 actgaatcag gcttggcgtg tcggttacga cattacttcc ggctaccgtg tccccaatgc
    11161 gtccgaagtg tatttcactt acaaccacgg ttcgggtaat tggctgccca atcccaacct
    11221 gaaagccgag cgcagcacca cccacaccct ctctctgcaa ggccgcagcg aaaaaggtac
    11281 tttggatgcc aacctgtatc aaagcaatta ccgcaatttc ctgtctgaag agcagaagct
    11341 gaccaccagc ggcactcccg gctgtactga ggaaaatgct tactacggta tatgcagcga
    11401 cccctacaaa gaaaaactgg attggcagat gaaaaatatc gacaaggcca gaatccgcgg
    11461 tatcgagctg acaggccgtc tgaatgtgga caaagtagcg tcttttgttc ctgagggctg
    11521 gaaactgttc ggctcgctgg gttatgcgaa aagcaaactg tcgggcgaca acagcctgct
    11581 gtccacacag ccgctgaaag tgattgccgg tatcgactat gaaagtccga gcgaaaaatg
    11641 gggcgtgttc tcccgcctga cctatctggg cgcgaaaaag gtcaaagacg cgcaatatac
    11701 tgagtatgag ggctaccgat ttatcaaaaa agtcaaagat tatccgtggt tgaataagtc
    11761 ggcttatgtg ttcgatatgt acggcttcta caaaccggtg aaaaacctga ccctgcgtgc
    11821 gggcgtgtac aacctgttca accgcaaata caccacttgg gattccctgc gcggtttgta
    11881 tagctacagc accaccaatg cggtcgaccg cgatggcaaa ggcttagacc gctaccgcgc
    11941 cccaggccgc aattacgccg tatcgctgga atggaagttt taatctagta ttattggatt
    12001 aattgtctcg ttggaaatta aagccgtccg aattgtgttc aagaactcat tcggacggct
    12061 ttttaccgta aatctgtgtg atgggg
//
LOCUS       DAWFJR010000056        11893 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWFJR010000056 DAWFJR010000000
VERSION     DAWFJR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11893
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.056"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..412)
                     /locus_tag="WGR04_001394"
     CDS             complement(<1..412)
                     /locus_tag="WGR04_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2,9-polysialyltransferase"
                     /protein_id="HEZ0794931.1"
                     /translation="MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQ
                     LNQVQSLIKIQKLTNNLLVILYTSKNLKMPKLVHQSANKNLFESIYLFELPRSPNNIT
                     PKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYL"
     gene            complement(496..1545)
                     /gene="siaC"
                     /locus_tag="WGR04_001395"
     CDS             complement(496..1545)
                     /gene="siaC"
                     /locus_tag="WGR04_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HEZ0794932.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIE"
     gene            complement(1546..2232)
                     /gene="neuA"
                     /locus_tag="WGR04_001396"
     CDS             complement(1546..2232)
                     /gene="neuA"
                     /locus_tag="WGR04_001396"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEZ0794933.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            complement(2236..3369)
                     /gene="neuC"
                     /locus_tag="WGR04_001397"
     CDS             complement(2236..3369)
                     /gene="neuC"
                     /locus_tag="WGR04_001397"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEZ0794934.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTYKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGATIGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKYQSDKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHLGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            3496..4671
                     /gene="ctrA"
                     /locus_tag="WGR04_001398"
     CDS             3496..4671
                     /gene="ctrA"
                     /locus_tag="WGR04_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ0794935.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            4686..5849
                     /gene="ctrB"
                     /locus_tag="WGR04_001399"
     CDS             4686..5849
                     /gene="ctrB"
                     /locus_tag="WGR04_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HEZ0794936.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            5849..6646
                     /locus_tag="WGR04_001400"
     CDS             5849..6646
                     /locus_tag="WGR04_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0794937.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLKADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGDSVTTYENPWYILLCNLVLLLLGLAVVARFSKGVEPQ"
     gene            6643..7293
                     /locus_tag="WGR04_001401"
     CDS             6643..7293
                     /locus_tag="WGR04_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0794938.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            7357..9630
                     /locus_tag="WGR04_001402"
     CDS             7357..9630
                     /locus_tag="WGR04_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ0794939.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIACRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALGRLKEAADTDAITVFARNLKDLLLVAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGITAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIREISDLQIGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNRFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEAGGTAKGNRSSETRHQERRDRKPQRND
                     RAPTNSAMADAFAKLKR"
     gene            9661..10663
                     /gene="dcm"
                     /locus_tag="WGR04_001403"
                     /pseudo
     CDS             9661..10663
                     /gene="dcm"
                     /locus_tag="WGR04_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            10656..11699
                     /gene="galE"
                     /locus_tag="WGR04_001404"
     CDS             10656..11699
                     /gene="galE"
                     /locus_tag="WGR04_001404"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ0794940.1"
                     /translation="MNKQYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYAEK
                     FYFHDECQQLDLYRKNIGKLDNQYKRALAFTLMRRAMIRKMPYTEDMRPGDTANPYGA
                     SKAMVERMLTDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAS
                     GRLPQLSVFGGDYPTPDGTGMRDYIHVMDLAEGHIAAMKAKGGVAGVHLFNLGSGRAY
                     SVLEIIRAFEAASGLNIPYQIQPRRAGDLACSFADPSHTKQQTGWETKRGLQQMMEDS
                     WRWVSRNPNGYGD"
     gene            11700..>11893
                     /locus_tag="WGR04_001405"
     CDS             11700..>11893
                     /locus_tag="WGR04_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEZ0794941.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHIIRNTQDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVD"
BASE COUNT         3148 a         2525 c         2917 g         3303 t
ORIGIN      
        1 tcagatagga gtagtggcct gtaaaagaca gcatatagag atgagcaggc tgtataatat
       61 taaggatttt tttgtaactt ctataaatat aaagtaattt tttaggagtt atattattag
      121 ggcttctagg aagctcaaat agataaatag attcaaatag attcttgtta gctgattgat
      181 gaactaactt aggcattttt aagtttttag aagtatataa aattactagt aaattattgg
      241 ttaatttttg tattttaatt aggctttgga cttggttaag ctgacctaaa ttagatatga
      301 caaataaatt gttacgtggg ggggtaagat aaaatggaga tgttgtcaac cacattgaat
      361 cttgaaaaaa ctttttaggc tgaaaaagag ctttttttat tttctttagc attattgtat
      421 ctcttaaaaa ttaatgagaa ttagctatat gtaatagcca atcctctgtt aataaagtaa
      481 ctaagttaat aagcattatt caatatcagt ttttttgatt tgagcacctt tgcgaatatt
      541 gcaagcagcg accttaccaa ataatgtttc atattcgttg acgctgaagt ctccattgcc
      601 tgggcgttta acccataggt tatctccgga caacagttct ccttttttaa tgtctttatc
      661 tgctacgaca gatgcaaagg cgaaatcttt agttggcttt tctcccgcga taatcgtgtc
      721 ttttttgccg ccgcgtgcca attttaaagc atgagcgcct tgcttgagct ctttaaaagt
      781 atccggattc atagagcata caatatccgg acctgggcga tccatgcggt cagtaaagtg
      841 acgctctaaa atcgaaccgc ctaaagctac tgctcctaag caagcatagt tatctaaggt
      901 atggtcagac aggccaatga ttgcgtctgg aaaggcttca gataaatcgt tcataccacc
      961 caatcgaaca tcttcgtaag gggttgggta gatgttggta cagtgaagca aagcataagg
     1021 tacccctgct tctcgaataa tttctaccga ctttttgatg ctttcaatag aattcatgcc
     1081 ggtagagaga ataataggct taccaaaaga ggccaccagt ttaattaatg ggtagttatt
     1141 acattcgcca gagccgattt tatatgctgg aatatccata cgttgtaatc gtaaagcagc
     1201 tgcacgagag aaaggagtac tgataaaaat cataccctta ctctctacgt attcttttaa
     1261 tttaatctca tcttcttcat tcagggcgca acgttccata atttcataaa tagagacatc
     1321 tgcattgcct ggaatgactt gtttggcctc atcagacatt tcgtcttcaa cgatgtgtgt
     1381 ttgatgttta acaacttcag cgcctgcatt ataggcagca tcaaccattt caaaagctgt
     1441 ttttaaagag ccttcatgat tgatgccgat ttcacagata atcaatggtt cgtggttgta
     1501 acctactgaa cgattaccaa ttttaaattc gttgttgttt tgcatttagc tttccttgtg
     1561 attaagaatg ttttctgcct gttgtaaatc aagctcagta tcaatatcga tagagtcttg
     1621 atgagacata atataaagtt tggttggggc gataaaaaaa caattatttg caattagtga
     1681 agcagtatca ttaatgtaaa ttgcaccatt aggcctaaat gcctgaggta attgttggcg
     1741 aggctgctcc aaatcgctta gatggcgcat gggggcatat tcgccattat tgatttgaag
     1801 cagggttttt agtggatgat gctccattgg gcatgcagag acaacggatc cttttatttt
     1861 ctcatcaaat agagaaaaag cttcacgaat atgagcccct gtgcgtaatg gactggttgg
     1921 ttgtaatagg gttactgtgc cggaattact gccaattgtt tctaaagcat gtattacacc
     1981 tgaaatagag ctggctgtat cggaggccag ctctgcaggg cgtaggacga cttcgacacc
     2041 gaaattttta gcttcttctg caattaaccc gccatcagtc gaaacaatta tgcggtcaaa
     2101 acactttgat gatatagcag cattaattgt atgaccaagt aatgatatgc cattcatttt
     2161 ccggagattt tttaatggca atcctttgga gttttggcgc gcaagtataa ccgcaatatt
     2221 ttgtttttcc ataatttaaa gattcaaatc gataaaacgt ttttgagcag aaacattcca
     2281 cgtttcagga ttgttgatta cttcagcaaa tctttctgtg ctggtgcgag tatctccgcc
     2341 attaaaggta tcatctgctt caaatttgcc taaactgcat gcttgttgaa tcgcatcaaa
     2401 gatatttttc gtttcataat ctgtatgaat aatagacttt cctagatggc ggttactttg
     2461 gcgtgtacca acatcaattg aagggacacc gtagagagga gcttctctaa tacctgcact
     2521 tgagttgccg accataaatt tagcatgttt caataagact aaaaaatatt caaatcgaat
     2581 ggaaggaaat gcaataaatt tatcagattg atattttaat aattcttgca gaatactttc
     2641 agtgccagtg tcattattag ggtagatgct aatgatattt tggccactta attctaatgc
     2701 tttgaaatat tgggccgcat attgtggcat taaatgtgct tctgtagtca cggggtgaaa
     2761 catagaaata ccataatttt cgtatggtaa accgtaatat tctttgactt cttctaagga
     2821 tgggagggtg gaagaggcca taacatctaa atcgggggag ccgatgatgt gaatatgctt
     2881 tcttttttct cccatttgca ctaggcgagt gacagcttgt tcatttgcta ccaagtggat
     2941 atgagaaagt ttactaatag aatgacgaat ggagtcatct actgtaccag atagttcacc
     3001 accttcgata tggcaaacta aacggctgct taatgcacct atagttgcgc ctgctagtgc
     3061 ttctaaacgg tcgccgtgaa tcatgaccat atcaggttca atttcatcag atagacgaga
     3121 gataaacgta atggtattgc ctaaaacggc acccattggt tcaccttgga tttgatttga
     3181 aaacagatat gtatgttgat agttttctcg agttacttcc ttgtaggttc tgccatatgt
     3241 tttcatcata tgcataccag ttacaatcaa atgcaattca aggtctgggt gattttcaat
     3301 ataggctaat aaaggtttta gcttgccgaa gtcggctcta gtacctgtaa tgcaaagaat
     3361 tcttttcatg attttagaat ctataagcat aaggaagttg gaaagaagaa tactaattat
     3421 actctacgta ctcataaatt tatttcgatt aagtgctata attaggccat ttataattat
     3481 attaggattt ggcttgtgtt taaagtgaaa ttttatattc gtcacgcagt attattattg
     3541 tgtggaagtt taattgtagg atgctctgcg attccttcat caggccccag cgcaaaaaaa
     3601 attgtctctt tagggcaaca atctgaagtt caaattcctg aagtggagct gattgatgtg
     3661 aatcatacgg ttgctcagtt attatataag gctcagataa atcagtcatt cactcagttt
     3721 ggcgatggtt atgcttcggc tggtacgcta aatattggtg atgtattgga tattatgatt
     3781 tgggaagcgc cgccggcagt attgtttggt ggtggccttt cttcgatggg ctcgggtagt
     3841 gcgcatcaaa ctaagttgcc agagcagttg gtcacggcac gtggtacggt ttctgtgccg
     3901 tttgttggcg atatttcggt ggtcggtaaa acgcctggtc aggttcagga aattattaaa
     3961 ggccgcctga aaaaaatggc caatcagcca caagtgatgg tgcgtttggt gcagaataat
     4021 gcggcgaatg tgtcggtgat tcgtgctggg aatagtgtgc gtatgccgct gacggcagcc
     4081 ggtgagcgtg tgttggatgc ggtggctgcg gtaggtggtt caacggcaaa tgtgcaggat
     4141 acgaatgtgc agctgacacg tggcaatgta gtacgaactg ttgccttgga agatttagtt
     4201 gcaaatccgc gacaaaatat tttgctgcgt cgcggtgatg tggttaccat gattaccaat
     4261 ccctatacct ttacgtctat gggtgcggtg gggagaacac aagaaatcgg tttttcagcc
     4321 agaggcttat cgctttctga agccattggc cgtatgggcg gtttgcaaga tcgccgttct
     4381 gatgcgcgtg gtgtgtttgt gttccgctat acgccattgg tggaattgcc ggcagaacgt
     4441 caggataaat ggattgctca aggttatggc agtgaggcag agattccaac ggtatatcgt
     4501 gtgaatatgg ctgatgcgca ttcgctattt tctatgcagc gctttcctgt gaagaataaa
     4561 gatgtattgt atgtgtcgaa tgcgccgttg gctgaagtgc agaaattctt gtcgtttgtg
     4621 ttctcgccgg ttaccagtgg cgcgaacagt attaataatt taactaatta atgtgagtaa
     4681 ttaagatgtc tgagcaactt cctgtggcag ttgccactga aaccaaagcc gagcgtaaaa
     4741 agccgaaaaa gaaaagttgg attaaaaagc taagcccttt attttgggta acggtgatta
     4801 tccctacggt aatttcgttg gtgtatttcg gcttcttcgc ttccgatcgt tttacgtcgc
     4861 aatcgagctt tgtggtgcgc tcgcctaaaa gccaatcttc tctcaatggc ctgggtgcca
     4921 ttttgcaggg cacaggtttt gcccgtgcgc aagatgatat ttacacggtt ggggagtata
     4981 tgcgttcgcg ctcgtctttg gatgaactgc gtaaaatctt gccggtgcgt gagttttatg
     5041 aaaccaaagg tgatgcgttc agccgcttta atgggtttgg gttccgtggc gaggaagagg
     5101 ctttttatca atactataaa aatcaggtga tgatcaattt tgatacggtt tcgggtattt
     5161 ccacgttgaa tgtaacttcc tttgatgcgc tggaatctaa gaaaatcaat gaggctttgt
     5221 taaaacaagg tgaagcattg attaaccagt tgaacgatcg tgcacgtgct gatacggtgc
     5281 gctatgcgga agaagtagtg aaaacggcgg cagagcgggt aaaggaagcc tctcagaatc
     5341 tgacggatta ccggattgcc aatggcgttt ttgatttgaa agcgcaatcg gaagtgcaaa
     5401 tggggttggt ttccaagctg caagatgaat tgattgtgat tcaaacccag ctggatcagg
     5461 tgaaagcagt cactccggag aatccgcaga ttccgggttt gcaggcgcgt gagcagagct
     5521 tgcgtaaaga aattgaccaa cagttacgtg ccatttcggg cggtgggcat tcttcgttgt
     5581 ctaatcaggc tgccgaatat cagcgtgtgt atttggaaaa ccagttggca gagcagcagt
     5641 tggcagccgc catgacttct ttggaaagtg ccaaggttga agcagaccgt cagcagcttt
     5701 atttggaagt gatctcgcaa ccgagcctgc cggatttggc acatgagcct aaacggttat
     5761 acaacattgt tgccactctg attatcggct tgatggttta tggtattttg agcctgttga
     5821 ctgccagcat tcgtgagcat aaaaactgat gaaagccttg cataaaacat cattttggga
     5881 atctttagcc attcaaaggc gcgtaatcgg tgcgcttttg atgcgggaaa ttatcacccg
     5941 ttacggtcgc aataatattg gctttttatg gctgtttgtt gagccgttgc tgatgacatt
     6001 cgttatcgtc ttgatgtgga aatttttaaa ggcagaccga tattcaactt tgaatattgt
     6061 cgcatttgcg attactggct atccgatgtt gatgatgtgg cgtaatgcct caaaacgggc
     6121 agttgggtcg atttcttcaa atgccagctt gctttatcac cgcaatgtaa gagttttgga
     6181 taccatcttg gcgcgcatga ttttggaaat tgctggtgca accattgcgc agattgtgat
     6241 tatggcggta ttgattgcga ttggctggat tgaaatgccg gcagatatgt tttatatgct
     6301 gatggcttgg cttttgatgg ctttttttgc gattggtttg ggtttggtga tttgttcgat
     6361 tgcctttaat ttcgagccgt ttggcaagat ttggggcaca ttgacttttg tgatgatgcc
     6421 gttatcgggt gcattctttt ttgtacataa tttgcctccg aaagtgcaag agtatgcatt
     6481 gatgatcccg atggtgcatg gcacagaaat gttccgtgcc ggatattttg gcgacagcgt
     6541 cacgacttac gaaaatcctt ggtatatttt gttgtgtaac ttggttttgc tgctgcttgg
     6601 tttggcggtg gtggcgagat tcagcaaagg ggttgagccg caatgatttc agttgaacac
     6661 gtttccaaac gctatctgac ccgccaaggt tggcggacag tcttgcacga tattagcttc
     6721 aaaatggaga agggcgagaa aatcggtatt ctcggccgca acggtgcagg taaatcgacg
     6781 ctcatccgtt tgatcagtgg cgttgagccg ccgaccacgg gtgaaatcaa gcggacaatg
     6841 agtatttctt ggcctttggc attctccggt gcgtttcaag gcagtctgac cggtatggac
     6901 aatttgcgtt tcatctgccg gatttacaat gtcgatatcg attatgtgaa gaactttacc
     6961 gaagagtttt ccgagttggg gcaatatctt tacgagccgg tcaagcgtta ttcttcaggt
     7021 atgaaggcgc gtttggcatt tgctttgtcg ttggcggtag agtttgattg ctatttgatt
     7081 gacgaagtga ttgcagttgg tgactcgcgt tttgccgata aatgtaagta cgagttgttt
     7141 gaaaagcgca aagaccgttc catcatcttg gtgtcgcaca gccacagcgc catgaagcaa
     7201 tattgcgata atgcgatggt gctggaaaaa gggcatatgt accagtttga agatatggac
     7261 aaagcctacg aatattataa ttcgctgcct taaagcgatt gtttttaaat caggccgtct
     7321 gaaatttcag acggcctgtc cgttggaatt ctattgatga acattactca aattctttcc
     7381 caagaactct ccgcgactgc cgcgcaaatc accgccgccg tcgagctttt ggacgacggc
     7441 gcgaccgtgc cgtttatcgc ccgctaccgc aaggaagcga cgggcgggtt ggacgatacg
     7501 cagttgcgcc ggcttgccga gcggctgcaa tatctgcgcg agttggaaga gcgcaaagcc
     7561 gttgttttaa aaagcattga agagcaaggc aagctttcag acgacctcag ggcgcaaatc
     7621 gaagccgccg ataacaaaac cgcgctggaa gacctgtatc tgccctacaa acccaaacgc
     7681 cgcaccaaag cgcaaatcgc gcgcgaacac ggtttgcagc cgctggcgga cgtgttgctt
     7741 gccgagcagt cgcaggacgt ggaagccgcc gcacaaggct acctgaacga aaacgtcccc
     7801 gatgccaaag ccgcgttgga cggcgcgcgt gcgattctga tggagcagtt tgccgaagac
     7861 gcggaactca tcggcacgct gcgcgacaag ctgtggaacg aagccgaaat ccacgcgcaa
     7921 gttgttggag gcaaagaaac cgaaggcgaa aaattcagcg attattttga ccaccgcgaa
     7981 cctattcgtg cgatgcccag ccatcgtgcg ctagcggttt tgcgcggacg caacgaaggc
     8041 gtgttgaaca tcgcgctcaa atatcagccc gacgacacgc cgattacgca gcaaagcgaa
     8101 tacgagcaaa tcatcgcctg ccgcttcaag gtttcagacg gccacaaatg gctgcgcgat
     8161 accgtgcgtc tgacttggcg cgcgaaaatc tttttgtcgt tggaacttga agccctaggc
     8221 cgtctgaaag aagccgccga caccgacgcg attaccgtgt tcgcccgcaa tctcaaagac
     8281 ttgctgctcg tcgcgcccgc cggacggctg accacgctgg gtctcgaccc cggctaccgc
     8341 aacggcgtga aatgcgccgt ggtggatgat acgggcaagc tgctggatac cgtcatcgtc
     8401 tatttgcatc aagaaaacaa tatgttggca acgctgtcgc gcctgattaa acagcacggc
     8461 gtgaagctca tcgccatcgg taacggcacc gccagccgtg aaaccgacaa aatcgcgggc
     8521 gaactggtgc gtgaaatgtc gaaaatgggg ctgcataaaa tcgtcgtgtc cgaagccggc
     8581 gcgtcgattt attccgcgtc cgaactggcg gcgcgcgagt tccccgactt ggacgtttcc
     8641 ctgcgcggcg cggtgtccat cgcccgcagg ctgcaagacc cgcttgccga gttggtcaaa
     8701 atcgacccca aatccatcgg cgtggggcag tatcagcacg acgtaaacca aagccagctc
     8761 gccaaatcac tggacgcagt ggtcgaagac tgcgtgaacg ccgtcggcgt ggacgtaaac
     8821 accgcctccg ccccactctt ggcgcggatt tccggtttaa atcaaaccct tgcccaaaac
     8881 atcgtcgcct accgcgatga aaacggcgcg ttcgacagcc gcaaaaaatt gctgaaagtg
     8941 ccgcgtttgg gcgaaaaaac cttcgagcag gcggcgggct ttttgcggat taacggcggc
     9001 aaagagccgc tggacgcgag cgccgtccac cccgaagcct atcccgtcgt cgccaaaatg
     9061 ctggcgcaac aaggcattac cgccgccgaa ctcatcggca accgcgagcg tgtgaagcaa
     9121 atcaaagcat ccgacttcac cgacgaacgc ttcggcctgc cgaccattct ggacatcctg
     9181 tccgagctgg aaaaacccgg ccgcgacccg cgcggcgagt ttcagacggc atcgtttgcc
     9241 gaaggcatcc gcgaaatcag cgacttgcaa atcggtatga ttttagaggg cgtggtttcc
     9301 aacgtcgcca acttcggcgc gttcgtggac atcggcgtcc atcaggacgg cttggtgcac
     9361 atctccgccc tgtccaaccg tttcgtccaa gatccgcgcg aagtggtgaa agccggcgac
     9421 gtggtgaaag tgaaagtgct ggaagtcgat gccgcacgca aacgcattgc gttgaccatg
     9481 cgcttggatg acgaggcggg cggcacagcc aaaggcaaca ggtcgtctga aacccgacat
     9541 caggaacgcc gcgaccgcaa accccaacgt aacgaccgcg ccccgaccaa ttcggcgatg
     9601 gcggatgcgt ttgcgaagtt gaagcggtaa aataatcgaa gagtttatgg attttgactt
     9661 atgcacacac cacttaccta tattgacctt ttctcgggag caggaggcct atccttaggt
     9721 tttgaacaag ccggattcca acaattgctt tctgtagaaa tggagcctga ttattgtcag
     9781 acttaccgtg ccaacttctc ccatcatcaa ttactgcaaa aagatttgac cacactaacc
     9841 gaacaagatt taatcaattg tctcaacgga caatcagtcg atttggttat tggggggcca
     9901 ccttgtcaag gttttagtat ggcaggaaag attggacgga catttacaga tgacccacgc
     9961 aatcatttat ttaaagagtt tgttcgaata gttaaaattg tccgaccatg tttttttgtt
    10021 atggaaaacg tagcgcgact atatacgcac aactcaggta aaactcgtac tgagatcatt
    10081 caagcatttc agaatatcgg ttattcggta gaatgtaagg tgctgaatgc agccgatttc
    10141 ggtgttcctc agatacgtag ccgagtgata tttatcggga ggagggataa aggcaaaatt
    10201 tcctttcccg aacctttgca gatttcccat cagactgttg gatcagcaat aggacatttt
    10261 ccaaaactgg ctgctggcga aagcaatcca cacgttgcaa atcatgaagc tatgaatcat
    10321 tcggcacaaa tgttagaaaa aatggcattt gttaaaaatg gaggtaaccg taacgatatt
    10381 cctgaaccat tacgtccgaa aacaggtgat atccgtaaat acatccgtta caacagcaac
    10441 aaaaccagcc gtttgtatta caggagatat gcgcaaagtt tttcactatg aacagaatcg
    10501 ggcgttaacc gttcgtgaat tagctgcctt acaatctttc cctgataatt ttattttttg
    10561 cggcagcaaa attgcccagc agcagcaggt tggtaacgcc gtaccgcctt tattggcaaa
    10621 agctattgct gaaagtattt taaaaatgag tgaaaatgaa taagcaatat ccgaaaatta
    10681 actatatcgg taataaagag aaaatagctt cctggatttg tgaccagctt ccgtctgatg
    10741 tagatacagt tgcagatgta tttagtggag gctgttcctt tgcctatgaa gccaaaaaac
    10801 gcggctatcg tgtgattact aacgatattt tggcaattaa ttaccaaatt gctttagcat
    10861 taatagaaaa caaccatgaa acattaaatg acgatgatgt cgcaatgatt ttttcaggca
    10921 gcccgcatgc cggttttatg agtcagcgtt atgccgaaaa attctatttt cacgatgaat
    10981 gccaacaact tgatttgtac cgtaaaaata tagggaaact ggataaccag tataaacgcg
    11041 ctttggcgtt tactttaatg cgtcgcgcca tgatacgtaa aatgccctat acggaagata
    11101 tgcgcccggg cgataccgct aatccttatg gcgcatccaa agcgatggtg gaacggatgt
    11161 taaccgacat ccaaaaggcc gatccgcgtt ggagcgtgat tttgttgcgc tatttcaacc
    11221 cgattggggc gcacgaaagc ggcttgatcg gcgaacagcc caacggtatt cccaacaacc
    11281 ttttgcccta tatctgccaa gtggcttcgg gcaggctgcc gcaactgtcg gtattcggcg
    11341 gcgattatcc gacccccgac ggtacgggaa tgcgcgacta cattcatgtg atggatttgg
    11401 cagaagggca tatcgcggca atgaaggcaa aaggcggcgt tgccggcgta catttgttca
    11461 acttgggttc ggggcgcgcc tattccgttt tggaaatcat ccgtgccttt gaagccgcat
    11521 ccggcttaaa tattccctat caaatccaac cccgacgcgc cggagacttg gcgtgttcct
    11581 ttgccgaccc gtcccatacc aaacaacaaa ccggctggga aaccaaacgc ggcttgcagc
    11641 aaatgatgga agattcgtgg cgttgggtca gccgcaaccc caacggatat ggggattaaa
    11701 tgcaaaccgc aaacaaaaaa accatcctcg ttaccggcgg cgcgggcttt atcggttctg
    11761 ccgttgtccg tcatattatc cgaaacaccc aagattccgt cgtcaacctc gacaaactga
    11821 cctacgcggg caatctcgaa tcgctgaccg acattgccga taatccccgc tacgcttttg
    11881 agcaagtgga tat
//
LOCUS       DAWFJR010000057        11838 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWFJR010000057 DAWFJR010000000
VERSION     DAWFJR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11838
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.057"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(146..1438)
                     /locus_tag="WGR04_001406"
     CDS             complement(146..1438)
                     /locus_tag="WGR04_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ0794942.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQNLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLIYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            1516..2484
                     /locus_tag="WGR04_001407"
     CDS             1516..2484
                     /locus_tag="WGR04_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794943.1"
                     /translation="MKLTLAIPSLNLDKDETRIPLCLHAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSETVALATPVWQKMGMHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYASVLQQWEEHWFAP
                     IYEAVRTGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            complement(2685..4151)
                     /locus_tag="WGR04_001408"
     CDS             complement(2685..4151)
                     /locus_tag="WGR04_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0794944.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETWSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(4393..6567)
                     /locus_tag="WGR04_001409"
                     /pseudo
     CDS             complement(4393..6567)
                     /locus_tag="WGR04_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(7491..7736)
                     /locus_tag="WGR04_001410"
     CDS             complement(7491..7736)
                     /locus_tag="WGR04_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794945.1"
                     /translation="MLKSIEFNSHIRNRLAAYLKGRGLDFQTAMREEKGNKEIASIIH
                     GGLPVLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            7848..8276
                     /locus_tag="WGR04_001411"
     CDS             7848..8276
                     /locus_tag="WGR04_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ0794946.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDAPRGNPEYV
                     RLSDMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            8288..8764
                     /locus_tag="WGR04_001412"
     CDS             8288..8764
                     /locus_tag="WGR04_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ0794947.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GQRAWLTLKGYISELSRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(9114..9602)
                     /locus_tag="WGR04_001413"
     CDS             complement(9114..9602)
                     /locus_tag="WGR04_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ0794948.1"
                     /translation="MLKLTLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTSYAFVHYL
                     RY"
     gene            complement(9670..10725)
                     /gene="aroG"
                     /locus_tag="WGR04_001414"
     CDS             complement(9670..10725)
                     /gene="aroG"
                     /locus_tag="WGR04_001414"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ0794949.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGSNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAS"
     gene            11077..11282
                     /locus_tag="WGR04_001415"
                     /pseudo
     CDS             11077..11282
                     /locus_tag="WGR04_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697603.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11371..11649
                     /locus_tag="WGR04_001416"
     CDS             11371..11649
                     /locus_tag="WGR04_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794950.1"
                     /translation="MRGKTKVMTTQMCASRDVSKSAKGWSVGIGLNVGKQLTDSVGLE
                     FDPYYRHKTICKPREIVLDGDKTKMGRSKSNEYGFRVTATFYSQLKSK"
     gene            complement(11631..>11838)
                     /locus_tag="WGR04_001417"
     CDS             complement(11631..>11838)
                     /locus_tag="WGR04_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0794951.1"
                     /translation="LDNARFHRMGVLREMAEKLGHKVLPLAPYSPELNPIEKVWANIK
                     RYLQTVLSDYARFDDALLSYFDFN"
BASE COUNT         2676 a         3284 c         2904 g         2974 t
ORIGIN      
        1 cgtgccgtgg tgtccggatt cccgccggta caggaatgac agcagtgctt ccttggctta
       61 attttatttt aatcaactgc aaatgccgtc tgaaaaccgg tttgcagatt ttcagacggc
      121 attgttttcg tcgccggttc aaacttcaat ccgattgcga cacggtaacc ggtatgccgc
      181 gcaagcgcgc aagcagcgcg tccgccttct gtttctgcgc ggcaaacgcc gccttgtccg
      241 cctgcgtcaa ttcgggtgtc ggcaatgcga ctgaaacagg attgacgggc tgcccgttga
      301 tgcgtgcctc gtaatgcagg tgcggcccgg tcgaacgccc ggtcgaaccg acaaaaccga
      361 tgacctcgcc gccgcgcaca ttgccttgtg cctgcgaaaa cgcgctcaag tgcgcgtaca
      421 gcgtttccac gccgttggcg tggcgtatca tcaccgcgtt gccgtatccg cccttccggc
      481 ctttaaaggt aatcacgccg tcggcggaag ccctgaccgg cgttccctgc ggtgcggcat
      541 aatcgatgcc cgtgtgcagc cgccaagtgt gcaggatggg gtgcatgcgg tagccgaacg
      601 gcgaagaaat gcgcgtatag accagcggct cgatgttgaa gccgcctttt tcctgcaaca
      661 ccctgccgtc ttcatcgtaa taattgccgc cccctccgcc ttccttgtcc gaacggtaat
      721 agaacgcctg atgccttgtg ccgcccttaa cgacttcagc cgccaaaata tcgcccgccg
      781 ccacctgctg cccgtggaaa tacaggctgt cgtaaatcag gcgcacggca tcgccttcct
      841 tcaaaccgtc aaggctgaag cggccggcga aaatcccgct taaggattcg cggatttcga
      901 cgggcacttc cgcccgcgcc agcgaaccgc gcgccgacgt tttgaccacg accgaacgca
      961 gcgtcggcaa aaccttcata tccgcctcag aagccgaccg ccgccatatg ccgcctttct
     1021 tttccaaagc gaccagattg cgctcgccgt cttcgtcggt aaaaaactgc acttcgcgcg
     1081 cgccgccgtc gccgccgacc aaaacatgaa ccgactggtc agcacgcaaa tgccgcaaat
     1141 cggcttcgcc gccatatttt tccgtgattc gggcaatctc gtcccgcgcc atacccgaac
     1201 gcgccagcac gtccgccagc gagtcgcccg gctgcaccgc ctcctgcacc caatatgccg
     1261 tctgaacacc gctgccgccc caagacagcg gcggcagatt ttgttccacg cgctgcggtc
     1321 tgacgcgctc cgtcctctct gtcgaagcaa tgtatgccgc tgacaccaaa ataatcgaaa
     1381 cggcaagcgc gcgcagcgcg tatttccgat gttttgccga aagtgggaag acagccatat
     1441 tgttcctttt tgacggcgtt tgcgccgaat cctctataat gttgcgcctg acctgcattt
     1501 ctgaaacgtc aaacgatgaa actcaccctc gccattccct cgctcaattt ggataaagac
     1561 gaaacccgaa tacctctctg cctgcacgcc ttcaacaaaa tcctgcaata cggctcgctg
     1621 caccgccaaa gctgcacggc ttcggcattt tacgcgcgtt atttgtggtg cggcagactg
     1681 gcggaacgcg cggcgcaaag cctgaatatg ccgtctgaaa ccgtcgcgct cgccacgccc
     1741 gtctggcaaa agatggggat gcatcaggca aatgtgctga cggcggaata tttggatgtc
     1801 ggtacggatg aagcggaacg gctctgccgc gatctgtcgg cgttttacgg cgatattccg
     1861 tggcgtttcg ttcccgtcct gcccgaatta tggctggttt cccttccccg cgcgtaccgc
     1921 tggggcgcga agcccgtttt ggacttgggc ggactgttgg gtgcggacga tcaaccggac
     1981 ggggaagacg cgttggaatg gctgcgcgtc caaaccgaaa tccaaatgtg gctggcggcg
     2041 caccccgtca accataaccg caagaaacgc gggctgcccg agttgaacgg cttatggctt
     2101 tgggacagtc tgcacggaag cagtcaaggc ggcacgcttt tcgccgatac ggtttggagc
     2161 cgtttccacc ccgaccgccg cgcattgccc gacaacttcc gcgcctatga agaaacggcg
     2221 gcacacctgc ccgacacgca ccatatcctg tttatggacg atttgcgcct gaccgccctg
     2281 acaggggaca gggagcggta tgcatcggtt ttacagcagt gggaagaaca ctggttcgca
     2341 ccgatctacg aagccgtccg taccggcaaa atcaaacggc tggacatcac cacggacgga
     2401 caacacggcg gtacgctgac cttcaaaccc gccgacaggt ggaaattttg gcgcggcgcg
     2461 aaaacctttg acggaatctg gtgaacccgc ccgccgcaac cgcgacaatg ccgtctgaag
     2521 cccgaatcgg gtttcagacg gcattttacc tcgcatcgcc gttatgccgg aaaaaccgga
     2581 atgcgtttcc gcacacgcgg cggcacgcaa agcaccgccc gaaagccgcc gatgcccgcc
     2641 gtgataccgc cgtcggcagg gtttcctccg aaaaggaaca gcaatcagaa cgttttatta
     2701 aactcgacaa acgcccgatt tttctcgtat tcgttgaaca catcgttact ccacgtttcg
     2761 cggtgcgaca acgtcaggcg cggcgtgatg cctttgaaat gcaatgcccg gtgccaaagg
     2821 ctcaaggatg tgttcaattc tttatccctg cgcctttccc ctttaaaacc gctgaaaaag
     2881 ccgggttttt cataatgccg tttcgccgcg ccgaggcgca acagcgaaga caggccgctg
     2941 ccgccccatt cctgccccca ggcaaagcgc aggccgtaac ggttgaaatt gtcgccccgg
     3001 tcggcggggt tgcgctcgcg gtaaaaatcc aaaccgccca tccaatattg gcgcgcattc
     3061 cggtaaaaca ccagcgaatt ggaaatttgc aaatgggtat tgtcggaacg cgcccggcgc
     3121 gtattcttca aacgccccca ctccgccgaa gacaacgttt gccatttcgg ggtttgccaa
     3181 cggttgaaat aaaggcgtgc gccgttggtg taagaataag cgtcgttgcc gtaggtgcgg
     3241 cgttcgtgga acactgccag cccggcatct ttgcgccggt cggcaaaacc gatgccgccg
     3301 gaaacgcctg ccgtcatatc gttgaatttc ttattccccg gataaaccct gccggacacg
     3361 tcgccgcccg ccgtcgtgta ccagccgttt ttcagcgacc attttttctc cgcgccgagc
     3421 cggtaattga ccgccgtgcc gtccacctgt ttcgggaaag tccatttgcc gtactgctgc
     3481 cgtttcgggg cttggttgat attgtgttcg cgggtaacgc tgaagccgcc atttaccttc
     3541 cacgcatcgc gttcgcgcaa tgccttgcgg tacagctcga cctgctccat cagctgcggc
     3601 ggcaggtttt ccgccttcag gcggtcgaac tggtctgccg ccgcctcgtt ctgcctgttt
     3661 tcaaacaatg ccgccgccaa acgcatacgg acggcgggcg cgtcgggttg ggcggcaatc
     3721 aattcccggt aatgggaaat cgcctccttc accctaccgt ctgcctgcgc caaaatccct
     3781 tgtgcataaa gtgccaacat cttatcctgc tgcgcctgtt gtaggtaaat tggcaaaata
     3841 acgcggatac cggcaatatt gtttgaaacc actgcggaat acatcgcgcg ggacagtaat
     3901 tcgggatttt tcagcagggt ttctccgtca atctgcaaaa cttttccttt ttcccgcacc
     3961 tgccccggta ccttctccct gtcgattggt ttgacctccg cttcatgaag cctgaactcg
     4021 ggacggcttc tcaaatccgg ttcgcgcggt gtttcttcgg catatgccgc gcttgccaat
     4081 aaaggcagca gcagcatcca tctgtttcgt gcaggcataa atgtcttccc acaaaaataa
     4141 aaaataacca ttatcaatcg gcggattgta tcagtttttt atgccgctgt tccaaaccct
     4201 gccgcccgat gccgcgacaa tgccgtctga agccagaatc gggtttcaaa cggcggcaaa
     4261 aaaccggcgg ttttccgccg gtttcatcat gatgccccgc ccttcggggc ggcagggttc
     4321 ggattggttt gcggggcggc gcgcggggcc gtgccggtgt gccgcctgtg cgtcaaaaca
     4381 tttcccgacg catcaaaaca gccatttgag cgacaatgcg gcttctttgt cgccgtccgt
     4441 ccttttgccg tatccgatgc gtgcggacat atggcctttc caaccggctt caatgccgaa
     4501 ccgcccttcg agcgccgtcc tgcctgccag cgtctgtttt tcgccgtcca tttccacgcc
     4561 gaaagatttt gacctgtgca aaacattaaa agcggcaaaa ggctgaagat tgacaccatt
     4621 acgcaaagca aaacgggttt ttgcccgaat gccggcgcgg ctttgccact gaccgctgcc
     4681 gagcagtccg accgccgtcc cctcgctgtc ggtaaagccg ccgtttacgc ccaagtaggt
     4741 aaactgagcc tgcggttgca ggtaaaaccg cacattattg ccttttccga caacgccttc
     4801 cgccacaagc gcgttgtagc cgccttcgac agaagccgtc caacctttgg ttttgtagcg
     4861 ttccgcacgg ttttcatcat tgatgcggtg tttgaaacgt tggtattgca accagccgtc
     4921 caaatacgca ccagtttgtt tatcgcgcaa ctgatgccac gcagcataaa cacccccgcc
     4981 ataaccatgc aaataactgc ctgccgcacc gcctttgctg ttgactgatg cgtgctggtc
     5041 agccctgccg cccatcacac cgattgccag ccggctgcct tcattttgcc gtacaaacac
     5101 ctcgccgccg atttgcacgc ctttgcgccg cccgtccgca gccgcgccgc cccgtatatt
     5161 ttgatgcgac cggccgccga tgaagcgcag ccacaatttt tgacgggacg gatcggcggc
     5221 atacacgtcg tcgcccctgt cggcggcacg cagcccaaac aaagtatttg ccgcataagc
     5281 ctgttgggca tataaaaccg cttccgcctc agggatagcc aaacgctgca cgcccaagca
     5341 aaatccgcct tcgcattgct tgagcaataa ttcgtacagt ccctgccggt aattttgttt
     5401 aaacgcaaac gaattagggt ctgccttttc cgccgcaatc agtttgcgcc cgtcgagcgt
     5461 tttcagattt tccggccggc gttccaaaac cacattggat tttccgctga cgttttgttc
     5521 aaaggtcagg gcgggttcgt ccgactcccg cacgtcgtag ccgaacagga aatccgcgcc
     5581 gccctgatac gctttatgga cggtcagttt ttcatctttc gtgttcaggc ggatgcgggc
     5641 attatcggaa gtttccagcg tatggatgtc ggaatggcgg cgcggttccc aaagggaatc
     5701 tttcagcgtc atcttggtca gttccaacac ctgtccggcg acgtgggatt tgttttcgat
     5761 tttgacgtgt aattttttat tgaagatgtt gttgagacta gtctgtgatg tattactatg
     5821 aggagtgagt acggaatctt ctaccaattt attaattaaa tcggggtttt tctgtttgag
     5881 tatactaaat tttggtccaa gctgttctat atacgcctcc tcagtccgtt ttttattttc
     5941 ttcccaagct tcgggtcttg ttttgtataa atcctgtaat tgcttcttaa ccgcatcctt
     6001 taaattatcc ccgtaagtat ctgtaatata tttcttaaca acgggcatat tatcgacggc
     6061 gagtatgccg ctattgtagt ttttaccttg gatattgatt tcgtcatacc catattcgga
     6121 tgaattttga tcggttttac cgctggaaaa ataatgggaa gtgccgggtt tcggcttgtc
     6181 tgaaaatgaa aaatcggcat cgtcgccgat tttggtagaa cgagtatttg aaccaagctg
     6241 agctctgaac ataccgaagc aatcaaaaga tatgctgcac gggccgcgcc aaacgtggga
     6301 atttctgtct tccgccgctt ctaaaataaa tttgttttta atctcggctt ctttgacggg
     6361 atcgtacgca tccgaacctc aaacatatgg cgtgcaaaac agcgataaaa cagataatgt
     6421 taatagatta acccgaccgc ttgcttgctt gcttgcttgc ttgcttgctt gcttgcttgc
     6481 ttgcttgctt gcttgcttgc ttgcttgctt gcttgcttgc ttgcttgctt gcttgcttgc
     6541 ttgcttgctt tcagtgtcat gagaaatttt cctttgtcaa gtgttaaaat tataatgatt
     6601 atatactaca ttatactaca tcgcaatgaa aaatcaggaa aagcaaaaaa ccctccgccg
     6661 tcattcccgc gaaagcggga atctagaatt ttaatgccgc aagaatttat caaggatggt
     6721 cggggtttaa aggtctggat tcccgcctgc gcgggaatga cggtttagaa atcacccgaa
     6781 acaagaaaaa gaaccgaaac cgaacaagcc ggattaccgc ctgcgcggga atgacgggat
     6841 ttcaggttac tgtttttgat tttctgtttt gtggaaataa cgggattttg gatgatgtgc
     6901 atttatcgag aaaactaaaa accctccgcc gtcattcccg cgaaagcggg aatctagacc
     6961 tttcggtgcg gaaacttatc ggataaaacg gttttccctg catcctggat tcccactttc
     7021 gtgcgaatga cgggatatag gtttcactac ggacgtgttc agattaccgc ctgcgcggga
     7081 aggacgggat ttcaggttgc tgtttttgat tttctgtttt tgtgggaata acgggacttt
     7141 ggatgatgtg catttatcgg gaaaacaaaa aaccctccgc cgtcattccc gcgaaagcgg
     7201 gaatctagaa ttttaatgcg gcaagaattt atcggaaaaa actgaagttt aaagacctag
     7261 attcccgcct gcgcgggaat gacggtttag aaatcacctg aaacgagaaa aagaaccgaa
     7321 accgaacaag ccggattccc acctgcgcgg gaatgacggc tcaaaagtta cgaaacgaaa
     7381 aataaccgaa accagacaag tcggattccc gcgcgggcgg gaatgacgat aagcgtaatt
     7441 tcaatctgcc atattgtctt ttcacttgcc tgaaaacaga tttcagccgc ctatccctgc
     7501 atccggcggc tgatgtagtc ggcaatcaaa tcccgctttt cccaaaaaaa tttctgcatt
     7561 ttttgttcgg aatacagttt gcgaaccagt acgggcaggc cgccatgaat aattgaggca
     7621 atttctttgt tgcctttttc ctcccgcatc gccgtctgaa aatccaaacc cctgcctttc
     7681 agatatgctg caaggcggtt gcggatgtgg gaattgaatt cgatggattt gagcattttg
     7741 cctccgtttt gcggtaagga tttattcgat ttgtttatcc gcctgatttt atcacgtttc
     7801 ccaagcggtt tgcctgtaca ataccgcttt tcggcagggt gtgggcgatg atgttttctt
     7861 ggttcaagct gtttcacttg ttttttgtca tttcgtggtt tgcagggctg ttttacctgc
     7921 cgaggatttt cgtcaatatg gcgatgattg atgcgccgcg cggcaatccc gagtatgtgc
     7981 gcctgtcgga tatggcggtg cggctgtacc gttttatgtc ccctttaggt ttcggtgcgg
     8041 tcgtgttcgg cgcggcgata ccgtttgccg ccggctggtg gggcagcggc tgggtacacg
     8101 tcaaactgtg tttgggcttg atgcttttgg cttaccagtt gtattgcggc gtgctgctgc
     8161 gccgttttca ggattacagc aatgcttttt cacaccgctg gtaccgcgtg ttcaacgaaa
     8221 tccccgtgct gctgatggtt gccgcgctgt atctggtcgt gttcaaaccg ttttgacgga
     8281 gcatcttatg ccgatagaaa tcgaacgccg ttttttgatt ggaaacgaca actggcggca
     8341 atacgccgac gaaccgctgc tgttgaggca gggttatctg tctgttgaaa aagaacgcac
     8401 cgtccgtgtc cgtattgccg gacaacgggc gtggctgacg ctgaaaggct atatttcgga
     8461 actcagccgc agtgagtttg aatacgaaat cccgcttgcc gatgcggaaa agatgatgga
     8521 aacgatgtgt ccgtttaaga tggaaaaatg gcgttatccg gtcagatggg gcggcagcct
     8581 gtttgaaatc gatgtttttc ttggcgataa tgcgcctttg gtcgtcgcgg aaatcgagtt
     8641 gcccgacgag aatgccgatt ttgaccgtcc ggactggctg ggacgggaaa tcactgccga
     8701 cggtatgttt accaatgcct atttgagcag gcatccgttc tcaagctgga aaaatgccgt
     8761 ctgaagcggc ttcagacggc attctccgac aaccatcgca aatccaaaga cagcctctat
     8821 atccttattt ctgctttctt tgttccatta ttcccaataa atccccataa cctaaaatct
     8881 cgtcattccc gctcaggcgg gaatccggtt ctttaaactt cggttttttc cgataaattg
     8941 ctttagcttt tcgtttccgg attcccgctt ttgcgggaat gacgatggaa gattgttgtt
     9001 atttcggata aattacccta actttaaaat cccggattcc catcttcggg ggaataacaa
     9061 tattttctat ttcagcccat catttgcggg ggattttata gagagtttat atttcaatat
     9121 ctcaaataat gcacaaaagc atagctcgtg cctttgctgc tgacgcggcg ttccgtccgc
     9181 tctgcttctt tccaatgcgt ccggtctatt gcggggaaaa atgcatctcc ttccacagac
     9241 aaatccactt cggttatccg caaatcggtc gccaatggca tcgcttgtcc gtatatctgc
     9301 gcgccgccca taatgacggc ttcttccgcg cctgcgcaca atgccaatgc cgcctccaaa
     9361 cttgccgccg tttccgcgcc tgccgcgcaa taatccgcct gccggctgat gacgatgttc
     9421 ctccgtccgg gcaggggttt gacgggcagg gattcccacg ttttccgccc cataatgacg
     9481 ggtttgccca aggtataggc tttgaaaaat gcgaaatctt cggggatgtg ccaaggcata
     9541 gcattgcccg ccccgatgca caggttttcc gcacacgccg caattagggt taatttgagc
     9601 atatttcctc cacaaaaatg ccgtctgaac gcttcagacg gcattttatg acattctgcg
     9661 tcaaaaatct caactggcgc gcgccatacg ttttttgttt gcacctgcca acaatgccaa
     9721 cagttcttca gtcgcgcccc aaccgataca cgcatcggta atgcttttgc cgtacacttc
     9781 cggcttatcc tgtctgcctt cgaccaaatg gctttccacc atcacgccca taatattgct
     9841 cccgtcctgt tccaattggg cagcaatatc ttgtgccact tccatctgcc gggtgtaatc
     9901 cttgcggctg ttggcgtggc tgcaatctat catcagctta tcggttaccc ctgccgcacg
     9961 cagttgttcc gccgcctcgc tgacgtgtcc cgcatcataa ttcggctctt ttccgccgcg
    10021 caaaatgaca tgacagtcgg gattgccgcc ggtatggaca atggcggaat gtccggcctt
    10081 ggttacagac aagaaatgat gcgaatggct cgccgcaccg attgcgtcga tggcaatctt
    10141 caaattgccg tccgtaccgt ttttaaagcc gacggggcag gacagcccgc ttgccaattc
    10201 gcggtgaact tggctttcgg tcgtccgcgc accgattgcc ccccaagaaa tcaagtccgc
    10261 ataatattgc ggcgtaatca tatccaaaaa ctcggtagag gcaggcatac ccatattgtt
    10321 cagcgacaac aacaggctgc gcgcctgacg caaaccgaaa ttgatatcga acgtaccgtc
    10381 caaatgcggg tcgttaatca aacctttcca acccaccgtc gtcctcggct tctcgaaata
    10441 aacgcgcatc acaatcaaaa gctcgttttc atactgcttg cggagtttca acaaacgctc
    10501 cgcatattcc aacgccgctt tcggatcgtg aatcgaacac ggcccgataa taaccaacag
    10561 ccgcttgtcc ctgccgtgaa ccaaatcgga aatttcctga cgggtgcggt gaaccaagcc
    10621 cgaagcctct ttggaaatcg gcagctcgta aagatgggca atcggcggca acaactcttt
    10681 aacttcttta atcttaatat cgtctgtggg gtaatggtgt gtcatcttgc atctttcctg
    10741 atggtttttt atacgcggct taggttacat cgtttacctt gcacaaacaa gtgcaacata
    10801 ttactacaaa gctattttat ttcaaaataa ctgatatatc ttttttcata gattttaaat
    10861 ttcatatttt taatatttta ggaattttat tccaaagaaa aagaaacaga ttgataaaaa
    10921 caatacccgc cattcattcg gacaaaaccg cacggaacat ccgttttccc atttccacga
    10981 caatccggta cagaacaccc cgctgccgat tccgcctgca catccggcaa aatttcatac
    11041 acaaggaaaa tgttgaaaac aaaaagaact cattgcttga aaaaagattt aatgggaatg
    11101 cgggtatttc ccacactttt tccatttatt tatcctcatt ataggaagaa aaacatgaaa
    11161 aaaccctgct tgcactctca cttgcagccg tgtccgccgt tacggtagcc gatattcccg
    11221 agtctaacgg caaaggcaaa gccatcatcg gcgataagga aaaacaccgt taccaagcat
    11281 gaaaaccttg ctttgaaagt ccatggcgaa atccgccaag atttggggga cggatgatcc
    11341 ctaacccctt gggcggatgc atatgcagat ttgcgcggca aaaccaaagt gatgacgacc
    11401 cagatgtgtg cttcccgcga tgtcagcaaa agcgccaaag gttggagtgt cggtatcggt
    11461 ctgaatgtag gcaaacagtt gaccgacagc gtcggtctcg agtttgatcc atactaccgt
    11521 cacaaaacaa tctgcaaacc ccgtgagatt gttttggacg gcgacaaaac caaaatgggc
    11581 cgctccaaat ccaacgagta cggcttccgc gtaaccgcaa cgttctatag tcaattaaaa
    11641 tcaaagtagg acagtagtgc atcgtcaaat cgggcgtaat cagacaaaac ggtttgcaga
    11701 taccgcttaa tattcgccca cactttctca atcgggttga gttcaggtga ataaggtgca
    11761 agaggcaata ccttatgtcc caatttttcc gccatttccc gtaagacacc catacggtga
    11821 aatcgcgcat tatctaaa
//
LOCUS       DAWFJR010000058        11528 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWFJR010000058 DAWFJR010000000
VERSION     DAWFJR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11528
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.058"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(241..933)
                     /locus_tag="WGR04_001418"
     CDS             complement(241..933)
                     /locus_tag="WGR04_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0794952.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(1041..2234)
                     /locus_tag="WGR04_001419"
     CDS             complement(1041..2234)
                     /locus_tag="WGR04_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0794953.1"
                     /translation="MSDQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2363..3100)
                     /gene="pxpA"
                     /locus_tag="WGR04_001420"
     CDS             complement(2363..3100)
                     /gene="pxpA"
                     /locus_tag="WGR04_001420"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ0794954.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGQVKAVDGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3087..4016)
                     /locus_tag="WGR04_001421"
     CDS             complement(3087..4016)
                     /locus_tag="WGR04_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ0794955.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKL
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDYLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(4013..4660)
                     /gene="pxpB"
                     /locus_tag="WGR04_001422"
     CDS             complement(4013..4660)
                     /gene="pxpB"
                     /locus_tag="WGR04_001422"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ0794956.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4844..7051
                     /gene="uvrD"
                     /locus_tag="WGR04_001423"
     CDS             4844..7051
                     /gene="uvrD"
                     /locus_tag="WGR04_001423"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ0794957.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDSGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVVAFVRLIEALRNQVGQMPLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEEM"
     gene            7284..7796
                     /locus_tag="WGR04_001424"
     CDS             7284..7796
                     /locus_tag="WGR04_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0794958.1"
                     /translation="MSQVFKDFDLSSVWKTNSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIALIAVQNGGMFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWLEEWEYPPIGVYFANDPSGGHALFALDYRECGKNGEPKVVFVELAPDFETFIRSLR
                     HEDEFIDEEI"
     gene            complement(7986..8201)
                     /locus_tag="WGR04_001425"
     CDS             complement(7986..8201)
                     /locus_tag="WGR04_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794959.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNPQLVQELEQHLIEAYNLMDTTG"
     gene            complement(8209..8412)
                     /locus_tag="WGR04_001426"
     CDS             complement(8209..8412)
                     /locus_tag="WGR04_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794960.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(8325..8684)
                     /locus_tag="WGR04_001427"
     CDS             complement(8325..8684)
                     /locus_tag="WGR04_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0794961.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            8737..9114
                     /locus_tag="WGR04_001428"
     CDS             8737..9114
                     /locus_tag="WGR04_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0794962.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWNQAKRHLRKFNGIPKAHFELYLKECEW
                     RFNNSEIKVQISILKQLVKSSLS"
     gene            9313..9933
                     /locus_tag="WGR04_001429"
     CDS             9313..9933
                     /locus_tag="WGR04_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ0794963.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            9956..11407
                     /locus_tag="WGR04_001430"
     CDS             9956..11407
                     /locus_tag="WGR04_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ0794964.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
BASE COUNT         2829 a         3068 c         2818 g         2813 t
ORIGIN      
        1 cgtatgctac cgcatactgg cctttttctg ttagggaaag ttgcacttca aatgcgaatc
       61 cgccgacctc tttcagttac agcagcttga tccctttccc ttatccaacg ggggaaggct
      121 aggatagggt ggcttgcaaa tatacagaac aagggacaag agccaccctc tctccaaccc
      181 tctccctccg tacgggaggg ggtggattct cgcgggcgaa gcccacgcta cggttagcct
      241 ttaccccagc acaaacaatt cccgcccgtg cgccttcagc caacttttag cattgtcggt
      301 atgcggcgtc agcgtgttca ccaaatgcca aaagcgcgga ctgtggtcgg ggtggcggag
      361 gtggcagagt tcgtggatgc agacatagtc ggcgacgtat tcgggcgtgc cgatcagccg
      421 ccagttgagg cggatgccgg tgtgcgggcg gcatacgccc caaaaggttt tggcgttgct
      481 caggtctgtg gcggtgggcg tcagtcctgt ttcggctgcg tgtttttcaa ggcggggcag
      541 caggtattcg cgggcgcgtt cgttcaacag gcggcgcagg tggtcgattt gtgcggcggt
      601 ttcttttcgg ggaagcagga tttcagacga cgtgatacgg atatggcttt ggctgtgggt
      661 atccagcttg gtctttattc cccgatacca aatccactcg ggtaagtttg ggtgggaaac
      721 aggatgcacg ggcgttttgg caagcgtgtt ccgcaaaatc gtttcgtttg ccgccagcca
      781 gtttgctaac gcgtggtctt gaaaaaaggg tgggacgttg atgctgaccg tctgcatatt
      841 gacggggcgc agaatcagat ttttcttggc actgcgtttg agttcgattt cgatgcacaa
      901 accgtcggaa agagtatagg tgaagcgttt catagttgtg aataggtttc agaccggata
      961 catcgtctga aacaggaatt ttccatatca ggcggcaaac ttcggataat atacaaaatc
     1021 aaacatctgc gctacaaggt tcagccgaac aagccgccga tatatttgct gatggtgatg
     1081 gcgctgagta ctgccatcaa accgaccaca atcacgccgg aaacggtgag ccacagcggg
     1141 tgtttgtagt cgccgacaat tttggttttg taggcggcaa tcagaatcag accgagggaa
     1201 atcggtaaaa tcaggccgtt taatgcgcct acgaacacca gcacctgcgc cggtttgccg
     1261 atggtggaaa atacggcggt ggacacggcg ataaaggcaa taatccattt gtttttattg
     1321 cgttcgatag acgggctgag accggagaag aacgacaccg aagtataagc cgcaccaatc
     1381 accgaagtaa tcgaagccgc ccaaatcacc acgccgaaaa tcagcaggcc gatgtatccc
     1441 gccgcatatt caaacggtgt ggaagcaggg ttgtcgggat tgagctgtac gccttggctg
     1501 accacgccca aaaccgccaa aaacaataca atccgcataa tcgaggcaat caggatcgcc
     1561 cgcaccgagc tttggctcac ttccggcaac gccgatttgc ctttgatacc tgcgtccagc
     1621 agacggtgcg caccggcgaa ggtgatgtag ccgccgaccg tgccgcccac cagtgtaaca
     1681 atcgccattg catcgagttt ttccggcata aaggtatgca cggcggcatc tgccagcggc
     1741 ggattcgcct gccatgccac ataaaccgtc agcgcaatca ttacgaaacc catcacttgg
     1801 gcgaatttgt ccatcacttt gcctgcttct ttaaacagaa acacaccgat ggcaatcacg
     1861 ccgctgatca cggcaccggt ttccggtgac agtccggtca gcaggttcag acccaagcct
     1921 gcgccgccga cgttgccaat attgaacgcc aaaccgccca tcacaatcag cacagccaag
     1981 aaatagcctg cgccgggcaa gacctgattg gcaatatcct gcgcctgttt ttcggaaacg
     2041 gcgacaatcc gccaaatatt gagctgcgcc ccgatgtcga gcagaatcga gagcagaatc
     2101 acaaagccga aacttgccgc cagtgcttgg gtgaaggtgg cggtttgggt cagaaagccc
     2161 gggccgatgg cggaagtcgc catcaggaat gcagcgccga ttaaggcatt tctgcggttt
     2221 ttttgatcag acataatcgc ttatcctcta taaaattggt tgttgctgtg tttgggcgaa
     2281 acctgcggtt ttagctacgc agaaactcgc tttgctcgtt ttggcgaaac ctgcggtttt
     2341 cagacggcct atgaactgtt tttcaagcag aaactttgat gcctgccgcc agtagttcct
     2401 gccggatttt ttcggcaaac accacggcgt gcggcccgtc tccgtgcaga cagatgctgt
     2461 cggcttgcac ggcaaccagg ctgccgtcca ctgctttgac ctgcccgtcc cgcaccatct
     2521 gcaatacttg ggcgatggct tcttcgtcgc tgtccacctg cgcatcgggg cggctgcggg
     2581 gaaccagcgt accgtcgggc atatagcggc ggtcggcgaa tacttcagaa atcacaccca
     2641 agcctgcggc ttttccggct tccaagagca ggctgccgga aagtgccatc aatttcaatt
     2701 tcgggtcgaa atccgccaca attcgggcaa cggtatccgc cagcgcacgg tttttcgccg
     2761 cttgattgta cattgcgccg tgcggtttga cataagccat ttccaaaccc tgatcacggc
     2821 acaaggcctg caatgcgccc aactggtaat tcagacacgc ccgcaaatcg gcttcggaca
     2881 gattcatttc ggtacggccg aagttttccc gatcgggata gccggggtgt gctccgatgc
     2941 gcacgccgtt ttgttgggca tacgccaatg ccgcccgaat atcggcaatg ctgccggcgt
     3001 gttgggcgca ggcgatgttg gccgaagtaa tcagctgcaa caaggcttcg tcgctgccgc
     3061 agccttcggc gagatcggcg tttaaatcaa cctgcttcat gggtgattct ccgtatttgg
     3121 ttcagatagg cttggttttt gcgccgcagg gcggtggctt ctttcaagcc gattattttg
     3181 aatttgactt tgctgccgaa gcgcacctgt gccagcctgc ccaaatcggc ggcggcaacg
     3241 gtagcgattt tcggataacc gccggtggtt tgcgcatcgg ccagcaggat aatcggtttg
     3301 ccgccgggcg gcacctgcac ggttcctgcc tgaacagcgt gggacagcat ttccaaaggt
     3361 tgcgacaggg tcagcggctg tccgtcgaag cggtagccca tgcggttgct atcgctttgc
     3421 agcgtccacg tttcccgttc cagattcaga cgcccttttt cactgaaagc ggcatattcc
     3481 gacgaaggaa caaggtggac ggtatcggta aacggtatcg gggcaatgcc gactttggac
     3541 aattcctgcg cacctttgcc gatggggaga taatcgcctt tttgcagcat tctgccctga
     3601 tggccgccga aaccggcttt caggtcggtg cttctcgaac ccatcacttc cggcacatca
     3661 aatccgcccg ccacgcacac atagccgtac atgccctgca cggcacgcac cagtttcaag
     3721 gtctgccctt tgcgggcggt ataacgccaa tacgaataga ccggttcgcc gtccaattcc
     3781 gcctgataca cggcaccggt gagacaaaac ggcgtatccc gttcaaacac cagcattatc
     3841 ccgcccaaag cgatttcgat tgcggccgtg ccttcgtcgt tgcccaataa aatattgccc
     3901 gccgccaaag caaccgtgtc catcgcaccg gcatgaccga tgccgtaacg ccggtgtccg
     3961 tagcgtccgg tatcctgaat atgcgccggt gcctgcactg ccgaaacgtg aatcatggct
     4021 caatcctttc tgcaacaaag cggacttggt cacccgccgc cagcagggtc ggcggattca
     4081 aatcggctcg gaacaagggt aattcggttc tgccgataat ctgccagccg ccgggcgaag
     4141 cgaacggata cacaccggtc tgactgccgc cgataccgac cgaaccggca ggaacggacg
     4201 ttctcggcac ggcacggcgg ggcgtgtgca atgcttcggg caagccgccc agataaggga
     4261 aaccgggctg gaagcccatc ataaatacgg tataagtttg cgccgtatgg cggcggacga
     4321 tttcggaaat aaccgtctga tggaaagcag cgacttccgc caaatccggg ccgtattcgc
     4381 cgccgtagca gacgggaatt tccaccagtt tgccctgatg gtctgtaacg gcggtgtgtt
     4441 cccacacata ttgcaattca tcggcaagcg tcgccaaatc ggtatcgaaa cgggtaaaca
     4501 cggtcagatt gttcatgccg accaccactt cctcaatcct gtcgtgctgc ccgagcgcag
     4561 cggcaaacgc ccacaacttt tgctgtttgc ccagttcgga aggcgcattc agtcggcaga
     4621 ccaaagcgga ttcgctgatt ggtgtgatct ctattctcat ttgttgttca ttttggttat
     4681 gttttaatga atctatatgc aggggcggcg gtttgtcaat atcttctgtg ctgcatcatc
     4741 aaaccgtcga ttggaaaagt gctgccctgc cgctgcactt tttcagacga ccttaaaccg
     4801 tttctattaa aatagcgcat tccacttttc agacggcatc cttatgtttc ccgaccaatc
     4861 cgcccccaac ctgctgcaag gcttgaatcc cgaacaactc tccgccgtaa cctggccgcc
     4921 gcaatccgca cttgtgctgg cgggcgcggg cagcggcaaa acgcgcgtgc tgaccacgcg
     4981 catcgcatgg ctgttgcaaa gcggacaagc cagcgtgcac agcattatgg cggtaacgtt
     5041 taccaacaaa gccgccaaag aaatgcaaac ccgtttgggc gcgatgattc ccatcaacgt
     5101 ccgcgccatg tggctcggca cgttccacgg cctctgccac cgctttttgc gcctgcacca
     5161 ccgcgatgcc ggtctgccgt catcctttca aatcctcgac agcggcgacc aactttccct
     5221 catcaaacgc ctgctcaaaa gcctcaacat cgccgaagaa atcatcgcgc cgcgttcgct
     5281 gcaaggcttt atcaacgcgc aaaaagaatc cggtttgcgc gcttccgtgt tgagcgcgcc
     5341 cgatccgcac acaagccgca tgatcgaatg ctacgccgaa tacgacaaaa tctgccaacg
     5401 cgaaggcgtg gtcgatttcg ccgagcttat gctccgcagc tacgaaatgc tgcaaagcaa
     5461 cgaaatcctg cgccagcatt accaaaaccg cttcaaccac attctcgtcg acgagttcca
     5521 agacaccaac aaactgcaat acgcctggct caaactcatg gcgggcggaa acgcagcggt
     5581 atttgccgtc ggcgacgacg accaaagcat ttaccgtttc cgtggcgcaa acgtcggcaa
     5641 catgaccgcg ctgatggaag aattccacat cgacgcgccc gtcaaactcg aacaaaacta
     5701 ccgctccgtc ggcaacatcc ttgccgccgc caatgccgtt atcgaaaaca acgacgaacg
     5761 actcggcaaa aacctgcgca ccgacgccga agcaggcgac aaaattcgct actactccgc
     5821 ctttaccgac ctcgaagaag cccggttcat cgtggacgaa accaaagccc tcgaacgcga
     5881 aggctgggat ttggacgaaa tcgccgtcct ctaccgtagc aacgcccaat cccgcgttat
     5941 cgaacaaagc ctgttccgca gcggcatccc ctacaaaatc tacggcggct tgcgctttta
     6001 cgaacgccaa gaaatcaaac acgccctcgc ctacctgcgc ctcgccgtca atcccgacga
     6061 cgacaacgcc ctcttgcgtg tcatcaactt cccaccgcgc ggcatcggtg cacgtaccgt
     6121 cgaaaatctt cagacggcct caaacgaaca aggcatcacc ctctggcaag ccgcctgcaa
     6181 cgccggcgcg aaagccgcca aagtcgtcgc cttcgtccgc ctgattgaag ccctgcgcaa
     6241 ccaagtcgga caaatgcccc tgtccgaaat catcgtcggc atcctcaaag acagtggctt
     6301 gaccgaacac taccgcaccc aaaaaggcga caaccaagac cgcctcgaca acctagacga
     6361 actcgtcaac gccgccatcg agttcaaacc cgaagacagc aactttgaaa ccctgcctga
     6421 aaacatttca gacgaccccg ccttccccat cctcgctttc ttgagcaacg ccgccctcga
     6481 atccggcgaa aaccaagcag gcgcaggcga aaaagccgtc caactcatga ccgtccacgc
     6541 cgccaaaggc ttggaattca acgccgtctt cctcaccggc atggaagaag gccgcttccc
     6601 cagtgaaatg agccttgccg aacgcggcgg cctcgaagaa gaacgccgcc tgatgtacgt
     6661 cgccatcacc cgcgcccgca aacgcctcta cattacgatg gcgcaacaac gaatgctgca
     6721 cggacaaacc caattcggca tcgtctcccg cttcgtcgaa gaaatcccac ccgaagtatt
     6781 gcactacctg tccgtcaaaa agaccgccta cgacagctac ggcagcccgc gccaaaccgc
     6841 cgcgtccaaa gacaaaatca tcgacgacta caaacagccc caaacctacg caggcttccg
     6901 tatcggacaa aacgtccgcc acgccaaatt cggcaccggc gtgattatcg atgccgcaga
     6961 caaaggcgaa tccgcccgac tgaccatcaa tttcggcaaa cagggcgtga aagagttgga
     7021 taccaagttt gcgaaactgg aagagatgta gaagggagat ggcagagacc gtctgaaaac
     7081 gggaacaaca atttttgccc aaacctccgc cgtcattccc gcgcaggcgg gaatccggac
     7141 ctttcggcac ggaaacttat cggataaaag gtttctttag attccacgtc ctagattccc
     7201 gccggaacat aaatgacgga cggtaaaagc cgggtatgaa tacccaccct ctgttatcac
     7261 tgagatcaat aaggaagaac attatgtccc aagtttttaa agattttgac ttgtcctccg
     7321 tatggaaaac taatagttgg gcagatgaaa actacaaaga agccccgttt acccctgaaa
     7381 ttttggctgc cgtagaaagt gagctaggct ataaattgcc gcaaagtttt attgcattga
     7441 tagcagtaca aaacggcggg atgtttgtca aaaactgctt tccgaccaca cagagaaatt
     7501 cgtgggcgaa agatcatgtg caaatttgcg aggtatcggg aatcggtttt gaaaaagaag
     7561 gaagcctgtg cggcgaaaca gggcaaaaac tttggctgga agaatgggag tatccgccca
     7621 tcggcgtgta ttttgccaac gacccgtcag gcggtcatgc cctgtttgcc ttggactatc
     7681 gggagtgcgg caaaaacgga gagccgaaag tggtgtttgt cgaactcgcc cccgattttg
     7741 aaacctttat ccgcagcttg cggcatgaag atgagtttat tgacgaagaa atataaaacg
     7801 gcggttgaaa aactgaaatc attaagtgaa aacgggcgaa ataacgggta accgtttgaa
     7861 tccgtaaggg aaacagttcg gcggaacgtg ccatccaaga cctttgcaaa gaaaaactgt
     7921 ccccgacagc attgacatta ttaacagaac ttatcaaatt tggagctatc ccaaatataa
     7981 ttcggttatc ctgttgtatc cattagatta tatgcttcaa ttaaatgttg ttctagctct
     8041 tgcactaatt gaggattgcg aattcctgat acaatctcaa attcttctgc atctatgcaa
     8101 acacctgcat aaatttcaat aacaagggaa cgcaataatt gaagctcttc tcttgttaaa
     8161 gaaataataa tgtcatcacc tgtgtaattg attatattca taataatttt atttttgctt
     8221 gtcaaagtaa gttttaccta aggttggtct aaatgcagtc ccaccatctt ttgaatttgg
     8281 gtctctaatt acaattgctc cagacttatc atcccaaatt gctcttatgt gtttggattg
     8341 taatcttcga attcccaaga aaaaaatcgt aataagtttg aaagtgtcaa atcccaagtt
     8401 tcttttgagc aatattctaa tattttatca atttcacttt taataatctt atgatcaaac
     8461 tgttctaata ttaatgcatt agaccaaaaa aaaccttctt tattacaatg atgggaaatc
     8521 catttaggag aacaaatgca aagtgaaaaa atagatgagc cttgttctcc ttcgattccg
     8581 atatccaaat ctatccatct atggaaatta tctggaattt cgggggtaaa tttttcaaaa
     8641 tcaatatcat ataaatttat gctttttaaa tccaatttaa tcattagggc tgtcctagat
     8701 aaatagggaa attcaaatta agttagaatt atccctatga gaaaaagtcg tctaagccgg
     8761 tataaacaaa ataaactcat tgagctattt gtcgcaggtg taactgcaag aacagcaaca
     8821 gagcctgaca gcattgttta tacggattgt tatcgtagct atgatgtatt agatgtgagc
     8881 gaatttagcc atttccgtat caatcacagc acacattttg ctgaacgaca aaaccatatt
     8941 aatggaattg agaacttttg gaaccaggca aaacgtcatt tacgcaagtt taacggcatt
     9001 cccaaagcgc attttgagct gtatttaaag gagtgcgaat ggcgttttaa caacagtgag
     9061 ataaaagttc aaatttccat tttaaaacaa ttagtaaaat cgagtttatc ttagttgtcc
     9121 aggacagccc cattattttt ataacaccgt gaagccgcac agcagtttga acagtgatac
     9181 gccgtttgcg ggcttacgag tttattttcc cggcctgcag tttgagcaat acggtgattt
     9241 cctacggtta atacaaatgt ttacacattg atacatttca tttatagttc cgcctatttg
     9301 aaaatagaaa atatgaattc gaccgcaagt aaaaccctga aaggattgtc gctggtgttt
     9361 ttcgcctctg gattctgcgc cctgatttac caggtcagct ggcagaggct tctattcagt
     9421 cacataggta tcgatttgag ttcgattact gtcattattt ctgtatttat ggtcggcttg
     9481 ggtgtaggtg cgtatttcgg tggacgcatt gctgaccgtt ttccttcaag tatcatcccc
     9541 ctgttttgca tcgctgaagt atccatcggt ctgttcggtt tggtaagcag gggtctgatt
     9601 tccggcttgg ggcatctttt agttgaggct gatttgccca tcatcgctgc tgccaatttc
     9661 ctcttattgc tgcttcctac ctttatgatg ggcgcgacct tgcccttgct gacctgtttt
     9721 tttaaccgga aaatacataa tgttggcgag tctatcggta ccttatattt tttcaacact
     9781 ttgggtgcgg cactcggatc gcttgccgcc gccgaatttt tctacgtctt ttttaccctc
     9841 tcccaaacca ttgcgctgac agcctgcttt aaccttctga ttgctgcttc agtatggctg
     9901 cgttacagaa aggatggata tagtgaacac taaaccgaat actagtttga tttatatgct
     9961 ttctttcctt agcggcttat tgagcttggg tatagaagtc ttgtgggtaa ggatgttttc
    10021 gttcgcagca cagtccgtgc ctcaggcatt ttcatttact cttgcctgtt ttctgaccgg
    10081 tatcgccgtc ggcgcgtatt ttggcaaacg gatttgccgc agccgctttg ttgatattcc
    10141 ctttatcggg cagtgcttct tgtgggcggg tattgccgac tttttgattt tgggtgctgc
    10201 gtggttgttg acgggttttt ccggcttcgt ccaccacgcc ggtatcttca ttaccctgtc
    10261 tgccgtcgtc agagggttga ttttcccgct cgtacaccat gtgggtacgg atggcaacaa
    10321 atccggacga caggtttcca atgtttattt cgccaacgtt gccggcagtg cattgggtcc
    10381 ggtccttatc ggctttgtga tacttgattt cttgtccacc caacagattt acctgctcat
    10441 ctgtttgatt tctgctgctg tccctttgtt ttgtacactg ttccaaaaaa gtctccgact
    10501 gaatgcagtg tcggtagcag tttccctaat gttcggcatc ctcatgttcc tactgccgga
    10561 ttctgtcttt caaaatattg ctgaccgtcc ggataggctg attgaaaaca aacacggcat
    10621 tgttgcggtt taccatagag atggtgataa ggttgtttat ggggcgaatg tatacgacgg
    10681 cgcatacaat accgatgtat tcaatagtgt caacggcatc gaacgtgcct atctgctacc
    10741 ctccctgaag tctggcatac gccgcatttt cgtcgttgga ttgagtacag gttcgtgggc
    10801 gcgcgtcttg tctgccattc cggaaatgca gtcgatgatc gttgcggaaa tcaatccggc
    10861 ataccgtagc cttatcgcgg acgagccgca aatcgccccg cttttgcagg acaaacgtgt
    10921 tgaaattgta ttggatgacg gtaggaaatg gctgcgtcgc catcctgatg aaaaattcga
    10981 cctgattttg atgaatacga cttggtactg gcgtgcctat tccaccaacc tgttgagtgc
    11041 ggaattttta aaacaggtgc aaagccacct taccccggat ggtattgtaa tgtttaatac
    11101 cacgcacagc ccgcatgctt ttgctaccgc cgtacacagt attccctatg cataccgcta
    11161 tgggcatatg gtagtcggct cggcaacccc ggtagttttc cctaataaag aactgctcaa
    11221 gcaacgtctc tcccggttga tttggccgga aagcggcagg cacgtatttg acagcagcac
    11281 cgtggatgct gcagcacaaa aggttgtctc tcgtatgctg attcagatga cggaaccttc
    11341 ggctggggcg gaagtcatta ccgacgataa tatgattgta gaatacaaat acggcagagg
    11401 gatttaaccg tcttaaaggg tttcaggcaa cgcaggtttt aggtaacgtc ctgctagttc
    11461 aaaaaaaacc gcatcacagc agtcgggaca aaatggttta aacattttgt cccgaattct
    11521 tattccta
//
LOCUS       DAWFJR010000059        11306 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWFJR010000059 DAWFJR010000000
VERSION     DAWFJR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11306
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.059"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(491..652)
                     /locus_tag="WGR04_001431"
     CDS             complement(491..652)
                     /locus_tag="WGR04_001431"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794965.1"
                     /translation="MPSELQTAFPASQLQRERTQTVIPAKNRKSKKKPKIRHSRADGN
                     PIRPVSVFF"
     gene            complement(665..1249)
                     /gene="rnhB"
                     /locus_tag="WGR04_001432"
     CDS             complement(665..1249)
                     /gene="rnhB"
                     /locus_tag="WGR04_001432"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ0794966.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAAAWHVAAATPEEIASLNILHATMLAMKRAVDGLAVRPERIFIDG
                     NRIPEHLGIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQTLF"
     gene            complement(1318..3213)
                     /gene="mnmG"
                     /locus_tag="WGR04_001433"
     CDS             complement(1318..3213)
                     /gene="mnmG"
                     /locus_tag="WGR04_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ0794967.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLSHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGERISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNADMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIAGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGCKIGLVGEAQWRMFNEKREAIEREIQRLKTTWYTPQKLAEGEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGITPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(3538..4965)
                     /locus_tag="WGR04_001434"
     CDS             complement(3538..4965)
                     /locus_tag="WGR04_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0794968.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            5125..6117
                     /gene="pdxA"
                     /locus_tag="WGR04_001435"
     CDS             5125..6117
                     /gene="pdxA"
                     /locus_tag="WGR04_001435"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ0794969.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGASEEGVLEVLHIPAAEAVEAGKLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLNKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            complement(7021..9780)
                     /locus_tag="WGR04_001436"
     CDS             complement(7021..9780)
                     /locus_tag="WGR04_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ0794970.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAGRADEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETATVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSENATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVLAGRGLRRSEQPKPSEAATVPAEEMIQV
                     ETRQG"
     gene            10274..11266
                     /locus_tag="WGR04_001437"
     CDS             10274..11266
                     /locus_tag="WGR04_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0794971.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAERKMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVRLPLSKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKVGLPPDLAQFAVMLENGTKM"
BASE COUNT         2209 a         2878 c         3433 g         2786 t
ORIGIN      
        1 tctgtcggta cggaaactta tcgggaaaaa cggtttttcc aaccctgaga ctccggattc
       61 ctgttttcgc gggaatccgg ttttttgagt ttcagtcatt tttgataaat tcttgcagct
      121 ttgagtttct agattcccgc ttttgcggga atgacgcgga aaagttgctg tgatttcgga
      181 taaattttcg tcacgcttaa tttctgtttt atccgataaa tgcctgcaat ctaaaatttc
      241 gtcattcccg caaaaacaaa aaatcaaaac agaagcctaa aatttcgtca ttcccgcgaa
      301 ggcgggaatc taggtctgtc ggtacggaaa cttatcgggt aaaacggttt tgccagccct
      361 gagactccgg attcctgttt tcgtaggaat ccggtttttt gagcttcagt catttttgat
      421 aaattcttgc agctttgagt ttctagattc ccgctttcgc gggaatgacg gtttggaagt
      481 tacctgaaat tcaaaaaaaa acggaaaccg gacggattgg attcccgtct gctcgggaat
      541 gacggatttt aggtttcttt tttgattttc tgtttttcgc aggaatgacg gtttgggttc
      601 tttctctttg aagttgcgat gctggaaatg ccgtctgaag ttcagacggc atttttgtgc
      661 cggtttaaaa cagcgtctgc tgcgcgagca ggtttctgac gggggcgaag tcgcggcgat
      721 gttcgggcag cacgccgtat tgtttgaggg cttccagatg ctgcttcgtg ccgtaacctt
      781 tgtgtttgtc gaaaccgtat tggggatggc gttgcgccag tgcgtacatt tccgcatcgc
      841 gtgcggtctt tgccaaaacg gatgcggcgg agatttcgat gattttgctg tcgcctttga
      901 cgacggcttc ggcagggata cctaaatgtt ctggaatgcg gttgccgtcg atgaatattc
      961 tttcgggacg cacagccaaa ccgtcaacgg cgcgtttcat ggcgagcatg gtagcgtgca
     1021 ggatgttgag gctggcgatt tcttcgggcg tggcggcggc gacgtgccac gcggccgcct
     1081 gattttttat catttcggca agcgcgtcgc gttttttctc gctgagtttt ttggagtcgg
     1141 tcagtccggg caggtcgaat gtttccggaa ggatgacggc ggcggcaaac acgctgccga
     1201 ctaaaggccc gcgtcctgcc tcgtccacgc cggcggtcag tatgtgcatg atgtttcctg
     1261 tcgggatagt aggaatgccg tctgaaaagg gtttcagacg gcatcgtacc gatgtgttta
     1321 tttcgcgtct ttaaacccgc gcttcaaatg caccatcagc aatgccactg ccgcaggggt
     1381 tatgccggaa atgcggctgg cttgtccgac ggtttcgggt ttgtgctgat tgagcttttg
     1441 ctgcacttct gccgacaagc ctttgacttt gccgtaatcg atgccgtcgg gcagttttaa
     1501 ggtttcgatg tcgcggcggc tgtcgatttc ttcgttttgg cggtcgatat agccttggta
     1561 tttgacttgg atttcgactt gctcgatgac ttcggcggag aggttttcag acggcatcgc
     1621 gccttcgagc gtcatcagcg cggcgtagtc gaggtttggg cggcgcagga ggtcgtgcag
     1681 gttggcttcg cggctgagtt tttgtccgaa cacacggatt tgttcgcctt cggcgagttt
     1741 ttgcggcgtg taccacgttg ttttcaaacg ttggatttcg cgttcgatgg cttcgcgttt
     1801 ttcgttgaac atacgccatt gcgcttcgcc caccaagccg attttgcagc cgtcttcggt
     1861 caggcgcata tcggcgttgt cttccctgag ttgcaggcgg tattcggcgc ggctggtgaa
     1921 cattcggtaa ggttcgttca cgcctttggt gatgaggtcg tccaccaata cgccgaggta
     1981 ggcttgttcg cggcgcagca ggagagggtc ttgttcgcgg acgtattgca cggcgttcgc
     2041 gcctgcgagc agtccttgcg cggcggcttc ttcataacct gtcgtaccgt tgatttgccc
     2101 ggcgaaaaac aatccggcaa tggttttggt ttcgaggctg gctttgaggt tgcgcgggtc
     2161 gaagtagtcg tattcgatgg cgtaaccggg gcgcaggata tgggcgtttt ccaaaccttt
     2221 catactgcgg acgagcgcga tttgaatgtc gaacggcagg ctggtggaga taccgttggg
     2281 gtagtattcg tgcgtggtca gaccttcggg ttcgaggaaa atctggtggc tgtctttatc
     2341 ggcgaaacgg ttgattttgt cttcgataga cggacaataa cgcgggccca cgccttcgat
     2401 tttgccggta aacatcgggc tgcggtcgaa gcctgagcgg atgatgtcgt gggtttgcgt
     2461 gttggtatgc gtaatccagc aggacacttg gcgcgggtgc atatcggcgt tgccgcgcac
     2521 ggacatgacg ggaacgggcg tgtcgccggg ctgttcggtc agttgggaaa aatcaatcgt
     2581 gcgtccgtcg atgcgcggcg gcgtgccggt tttcagacgg ccttgcggca gcttcaattc
     2641 gcgcaaacgt ccgcccagcg atttggcggc ggggtcgccg gcgcggccgc cttcgtagtt
     2701 ttccaaaccg atatggattt tgccggacaa aaatgtgcct gcggtcagca cgacggcgcg
     2761 ggctttgaat tccacgccca tcgcggtgat tacgccgctg atgcgttcgc cgtcgagcgt
     2821 tacgtcttcg acggcttgtt ggaaaaggtc gaggttttct tggttttcca acatctcacg
     2881 gatggcggct ttatacaaaa tgcggtcggc ctgtgcgcgc gtggctcgta ctgccgcgcc
     2941 tttgctggcg ttcaggcggc ggaactggat gccggatttg tcggttgcca acgccatcgc
     3001 gccgccgagc gcgtcgagtt cgcgtactaa atgccctttg ccgatgccgc cgatggaagg
     3061 gttgcacgac atttgtccga gcgtttcaat attgtgtgag agcaaaagcg tctgcgcgcc
     3121 catacgggcg gcggcgagcg cggcttctgt gccggcgtgt ccgccgccga cgacgataac
     3181 gtcgtaggtt ttggggtaaa tcatgtgggt catgtgtgta ttgcctgtcg gtgtttcaga
     3241 cggcatttat agtggattaa caaaaaccag tacagcgttg cctcgcctta gctcaaagag
     3301 aacgattctc taaggtgctg aagtaccaag tgaatcggtt tcgtactgct tgtactgtct
     3361 gcggcttcgt cgccttgtcc tgatttttgt taaaccacta tattcaatat gccgtctgaa
     3421 aaacgaaatg gattcaaaag taaagggttg ggattgtacg cttgttcgcc ctgtttttac
     3481 agtgtgcgga aagggaaaag ccgcttcgcg gggaagcggc tctggtaagg gcgggattta
     3541 ccaaacgtcg gatttgatac ggcgtttcag gcccggatgt tcggaaagtt tgaactcggg
     3601 gtctttgccc attttcagct tggcggtgta atcgcgcagc agcataaacg ccaagggcga
     3661 gagcagcagg atggcgacaa ggttgatcca cgccataatg cccatcgcca tatccgccat
     3721 atcccagacc aaaggcacat tggcaaccgc gccaaaatag acccacgcca aaaccagcat
     3781 acggaaaacg gcggtaatca gccaatggct tttgatgaat tggacgttgg actcggcata
     3841 ggcatagttg ccgataacgg tggaaaaggc aaacataaac aggatgacgg cgaggaagcc
     3901 cgcgccccat tgccccactt ggctgacaat cgccgcctgc gtcagcgccg caccgctcaa
     3961 atcgccgtaa ggctgttggt aaatcaagat gatgaaggcg gtgcaagaac aaacgatgat
     4021 ggtatcgaca aacacgccca gcatttgaat cataccttgc gaaacagggt gtttcacttc
     4081 ggcggcggcg gcggcgttcg gcgcggaacc catacccgcc tcgttggaat acaggccgcg
     4141 tttgatgccc atcatcatcg tttgcgaaat cagaccgccg agtaagccgc ctgctgccgc
     4201 gtcgaatttg aacgcgcccg aaaaaatctg accgaacacg tccggaatca tcggaatatt
     4261 ggtcaaaatg atgaaaagcg cgataaagag gtacaaaacc gccatcaggg ggacgacgat
     4321 ttccgccgct ttagatatgc gcctgatgcc gccgaagata atcggcgcgg ttaaaatcac
     4381 cagggcgacg ccgacataat gaggctccca accccatgcc gctttgacgg tatcggcgat
     4441 ggtattggtc tgaaccgctt caaacacaaa gccgaaacag aaaatcaggc tcagggcgaa
     4501 caacacgccc agccatttct gccccagccc ttgagtgatg tagtaggcag ggccgccccg
     4561 gaaatggtgg ttgtcgtagt cgcggacttt aaagagctgc gccagcgaag attcgacaaa
     4621 cgccgaactc ataccgatta aggcggttac ccacatccaa aacaccgcgc ccggtccgcc
     4681 gactttgatg gcgatggcca cgcccgcgat attgcccacg cccacgcggc tggcaaggcc
     4741 ggttacaaat gcctgaaacg gcgtgatgcc gtgagggtcg tccccctgtt tgcggccgcc
     4801 gagcatttct ttgatgctgc gcccgaacag gcggaattgg acaaagcccg tggttacggt
     4861 gaagaaaagc cccgtaccca aaagcatata aaccaagtat gaccacatcg gatcgttgat
     4921 ggcgccgacc cagccgtgca gccattcggt aaagttctcg ttcatatcgc ttccttaaag
     4981 ttgaaactcg cacatattgg cggtatgcaa gcagggttta aattttgtaa acgcccattc
     5041 tagcagattg tcaacaaaat cagaaaaatt tacatcgccg cgcggctgcg gcgttagaat
     5101 cgcattttgt ttggagcaaa cacgatgaaa cagcctgttt ttgccgttac ttccggcgag
     5161 cctgccggca tcggccccga tatttgtttg gacttggcat ttgcacgcct gccctgccgc
     5221 tgcgcggtat tgggcgacaa aaacctgttg cgcgcgcgcg ccgaagcctt gggcaaaagc
     5281 gtcgtcctgc gcgacttcga tccggaatca ggcggcgcgt ccgaagaagg cgtgctggaa
     5341 gtgctgcaca tccctgccgc cgaagcggtt gaggcgggca aactcaatcc cgccaacgcc
     5401 gcctatgtgc tgcaacttct agacaccgcg ctcgcaggca tttcagacgg cattttcgac
     5461 ggcatcgtta ccgcgccgct gaacaaaggc atcatcaacg acgcgcgcgc aagcacaggt
     5521 tttttcagcg gacacaccga atatctggcg gaaaaaagcg gcacggggca ggttgtgatg
     5581 atgcttgccg gcaaaggcct gcgcgtcgcc ctcgtaacga cccacctgcc gctgaaagac
     5641 gttgccgccg ccatcacgca accgctgatt gaaagcgtcg cacgcatttt gcatcacgac
     5701 ttaaaacaca aattcggcat caaaaatccc aaaatcctag tcgccggact caacccccac
     5761 gccggcgaag gcggacacct cggacacgaa gaaaccgaca ccattatccc cgcgctcgaa
     5821 aacctgcgcc gcgaagggat aaacctcgcc ggcccgtatc cggcggatac cttgttccaa
     5881 ccgtttatgc tcgaaggtgc ggatgccgta ttggcgatgt accacgacca agggctgccc
     5941 gtgttgaaat accacagctt cggacagggc gtgaacatca cgctcggcct gccctttatc
     6001 cgcacctccg tcgatcacgg caccgcgctt gatttggcgg caaccggcag ggcggattcc
     6061 ggcagcctga taactgccgt ggagaccgcc gtcgagatgg cgcgcggcag cctttaaaga
     6121 tgataaaaga cccgtcattt ccgcgcaggc gggaatccgg tctgttcggt ttcagttgtt
     6181 tttgggtttc gggtaatttc caaatcgtca ttcccgcgca ggcgggaatc cagaccttcg
     6241 ggcgacggca atattcaaag attatctgaa agtttgaggt tctagattcc cgttttcacg
     6301 ggaatgacga aaggttgcgg gaatgacggg aatggcgaag tttcagacgg catcgtccac
     6361 cctccgccgt cattcccgcg caggcgggaa tccaggcctt tgggcgacgg caatattcaa
     6421 agattatctg aaagtttaga ggttctagat tcccgttttc acgggaatga cggaatgttg
     6481 tgggaatcca gacctttggg cggcggcaat attcaaagat tatctgaaag tttgaggttc
     6541 tagattcccg ttttcacggg aatgacggaa tgttgcggga atccagacct tcgggcggcg
     6601 gcaatattca aagattatct gaaagtttag aggttctaga ttcccgtttt cacgggaatg
     6661 acggaatgtt gtgggaatcc agacctttgg gcggcggcaa tattcaaaga ttatccgaaa
     6721 gtttgaggtt ctagattccc gttttcacgg gaatgacgaa aggttgtggg aatccagacc
     6781 ttcgggcagc ggcaatattc aaagattatc cgaaagtttg aggttctaga ttcccgtttt
     6841 cacgggaatg acggaatgtt gcgggaatca tgggaatgac ggaatgttgc gggaatcatg
     6901 ggaatggcgg aatgtttcgg taatcacggg aatggcgaag tttcagacgg cattgcaggt
     6961 atccgaaccc atgtaaaaaa gaggttctgc ggaacagaac ctctttttgc cgccgtcggt
     7021 tcagccttgc cgggtttcga cttggatcat ttcttcggca gggacggttg cggcttcaga
     7081 cggcttgggc tgttcggaac ggcgcaaacc gcgtccggct aggacttcgg gttgtgccgc
     7141 ccatgccttc aatgcggcag ggtcggtttc gatcaggacg agtccgccgg tttgtgcggt
     7201 ttcccgtgcc tgttccgccg cagccgtaaa ggttgcgttt tcagacggca tttcctgtgc
     7261 ttcggctttc ggtgtcgcgc cttcgggcag gatggcggcg gtggcacggc ggattttttc
     7321 cgccgcatca taaaccggtg cgtcgccgtt tgaaacggcg ggagatgctg tcggaagatc
     7381 cctttctgca accggatcgg caatgctgac ggtaatcggc gcgtttgcgt cggtttcgcc
     7441 gaaaacgtgc gcggcggcgg aacggacttt gtcggcggtg tcgtgaatat tcaggtactg
     7501 ctcgattttg gcggcagacg gaatattgcg ttttttgccg ttttgacggc ggtcgcgctg
     7561 attgttgcgc tcgcggcgtt ctttggcatc tcggctgtcg cgttcgcggc ggttgcgttc
     7621 ggatttgggc ttgctgcctt tgtcttctgc ggtatgcggt tcggacggcg tgttttccgc
     7681 tgtctgaacg gttgtttcgg caacggtggc ggtttccggc gcggtttggc cgcgttcgct
     7741 gcggctgccg ttgcggcggc gttttccggt ttgcacttcg gtttcggacg gtgcggcatc
     7801 tgcaacggtt gcggcaggct gtatgttgcg gtcttggatt tccgcttcgt cggcgcgtcc
     7861 ggcggcacgg tcgccgcgtt cgttgcggcg gcggttgccg ttgttgcgcg tttcggcttt
     7921 gtcggcacgc gcttcctgtc cggcagtttt gcctgccact tcgcggactt ctactttgct
     7981 gccttcgcgt ttgctgcggc gcgggttttg gcggcggttg ttggcgcggc tgccgctgcg
     8041 gtttgccgtg ctgcgttttt cggaggtttc ggcagcgggc gcggcttggg tttcgctgcc
     8101 gccgaaaatg cgtttgagcc aagctttgaa gctgtcccac caggaggttt ttttctcggg
     8161 ggcggcagtc ggggcggggc tggtgtggcg cacgcctttg acggcgggtt cgggacgggc
     8221 ggctttggct ttttcgccgc cgaacggttt ggcggattcg tcttcttcag gctcggcgac
     8281 gcgtttgtag ctcggttcgc cgtcttcttc tacgtcgtcg gtgcggatgc ggttgatttc
     8341 gtagtgcgga ttttcgaggt ggatgttcgg aatcaggacg acgttgacat ccaaacgctc
     8401 ttccatcgca aacagctcgg cgcgtttttc gttcagcagg aaggtggcga catcgacggg
     8461 cacttgtgcg cgcacttctc cggtgttgtc cttcatcgct tcttcttgaa tgatgcgtaa
     8521 aacgtgcagg gcggtggatt cgatgccccg aatcacgccg gtgccggcgc agcgcggaca
     8581 ggcgacgtgg ctgctttcgc ccaaagccgg tttcaaacgt tggcggctca attctaaaag
     8641 tccgaaacgg gagagtttgc ccatctgtac gcgggcgcgg tcttttttga gcgcgtcgcg
     8701 caggacgttt tccacatcgc gctggtgttt ggggttttcc atgtcgatga agtcgatgac
     8761 gaccaagccg cccaagtcgc gcaggcgcat ttgtcgggcg acttcttcgg cggcttccat
     8821 attggttttg aacgcggtgt cttcaatgtc tgcgccgcga gtggcgcgtg cggagttcac
     8881 gtcgatggag acgagggctt cggtatggtc gatgacgatc gcgccgccgg agggcaggct
     8941 gacgctgcgc gaaaacgcgc tttcgatttg gtgttcgatt tggaagcggg aaaacagcgg
     9001 cgtgtggtct tcgtagagtt tcagacggcc tatattgccc ggcatgacgt agctcatgaa
     9061 ctcggcaact tggtcgtaaa cttcttgatt gtccaccaga atttcgccga tgtcggggcg
     9121 gaaatagtcg cggatggcgc ggatgagcag cgagctttcc ataaagagca ggtaagggtc
     9181 gtgatgcgct tttcctgctt cttcaatcgc ctgccagagt tgtttgagat agttcaaatc
     9241 ccattccaac tcttccgcgc tgcggccgat gccggcggta cgggcgatga tgctcatgcc
     9301 gttcggaatg tcgagttgcg ccatagcggc tttgagttct tgacgttctt cgccttcgat
     9361 acggcgggat acgccgccgc cgcgcgggtt gttcggcatc aataccagat agcgtccggc
     9421 gaggctgatg aaggtggtca gcgccgcgcc tttgttgccg cgctcgtctt tttcgacttg
     9481 gacgatgact tccatgcctt ctttgagcac gtcttggatg cgcgcgcgtc cgccttcgta
     9541 gtcttggaag tatgagcggg agacttcttt aaacggcaag aagccgtggc ggtcggttcc
     9601 gtaatcgacg aaacacgctt ccagcgacgg ctcgatgcgg gtaatgatgc ctttgtagat
     9661 attgcctttg cgctgttctt tgcccagcgt ttcgatatcc aaatccagca ggttttgtcc
     9721 gtcgacgatg gcaacgcgca gctcttcggc ctgcgttgcg ttgaataaca ttcttttcat
     9781 gatcacctcg tggacgggcg gcattcgggc gcacctgtcc ggttcggcat tccgtaaggc
     9841 tgggttttcc gatgttttcg gataaaaccg gtaatcagtt tttgagttga aaatccgcag
     9901 ggatgcacgt tccggagaac cgtgtgcgga agggtcggat acagaaggct ataaagatcg
     9961 atgcggcggt ttgtctgccg cgttccgaac gctgcggtcg gaaaaatggg ggccggcttc
    10021 ttcttgttat cgtgatgcct gtgttttggg cggtttgcgt ttgggacttg gggcccggct
    10081 gccgtcttac ttccgcgccg aaacggcaaa atcaattcaa acttgactac gttctgcgcc
    10141 tgccggctgg gaacaggcgc agggaaaatg ctttgcggag tgcgttttta atataaaatt
    10201 ccgttttaaa gtaaaccgtt tcaggggggc gcggcgggcg cgctttttgc tgaaacggat
    10261 gttcggatta tagatgaaaa cgcacgaaat aagcaaagat tcggtcagct tgataggggt
    10321 tgccgaacat gaggcgggtc aacgccttga taactatctg ataaaaatcc tcaagggtgt
    10381 tcccaagagc catatccacc gcattatccg cgccggcgag gtgcggttga acaagaaacg
    10441 ctgcaaaccc gacagccgta ttgcggaggg ggatacggtg cggattccgc ctgtgcgcgt
    10501 ggcggagcgc aagatgccgt ctgaaaggcg tgccgccgta ccggcgcgtg cgtttgacgt
    10561 tgtttacgaa gacgatgcgc ttttggtcat cgacaaaccg tccggcgttg ccgtccacgg
    10621 cggcagcggc gtgagtttcg gcgttatcga acagttgcgc cgcgcccgtc cggaggcgaa
    10681 gtatttggag ttggttcatc gtttggacaa ggatacgagc ggcttgttga tggtggcgaa
    10741 gaaacgcagc gcgctcgtca aacttcacga agccatccgt aacgaccacc ccaaaaaaat
    10801 ctaccttgcg ctgggggtgg gcaaactgcc ggacgataat ttccacgtca ggctgccgct
    10861 gtccaaatat acgggcgcac agggcgaaaa gatggtgcgc gtcagtgcgg acgggcagtc
    10921 ggcgcatacg gtgttccgtg tgttaagccg tttttcagac ggcattttgc acggtgtcgg
    10981 gctgtcgcac ctgactttgg tgcgggcgac gttgaaaacg gggcgcacgc accaaatccg
    11041 cgtccacctg caatctcaag gctgtccgat tgcgggcgac gaacgctacg gcgattatca
    11101 ggcgaaccgg cgtttgcaga agttgggttt gaagcggatg tttttgcacg cgtccgagct
    11161 gcacctgaac catccgctca cgggcgagcc gctggtgtta aaggtggggc tgccgccgga
    11221 tttggcacag tttgcggtga tgttggaaaa cgggacgaaa atgtgaaccc cgatgccgtc
    11281 tgaagccttc agacggcatc gggacg
//
LOCUS       DAWFJR010000060        11182 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWFJR010000060 DAWFJR010000000
VERSION     DAWFJR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11182
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.060"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            78..359
                     /locus_tag="WGR04_001438"
     CDS             78..359
                     /locus_tag="WGR04_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0794972.1"
                     /translation="MIHEEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDAATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            complement(380..1606)
                     /locus_tag="WGR04_001439"
     CDS             complement(380..1606)
                     /locus_tag="WGR04_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ0794973.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(1621..2046)
                     /locus_tag="WGR04_001440"
     CDS             complement(1621..2046)
                     /locus_tag="WGR04_001440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794974.1"
                     /translation="MPSEHAAYADLMASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSAGGIAGAAVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            2151..2810
                     /gene="queC"
                     /locus_tag="WGR04_001441"
     CDS             2151..2810
                     /gene="queC"
                     /locus_tag="WGR04_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ0794975.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            2838..3356
                     /locus_tag="WGR04_001442"
     CDS             2838..3356
                     /locus_tag="WGR04_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ0794976.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTKMPSENAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            3363..3785
                     /gene="queD"
                     /locus_tag="WGR04_001443"
     CDS             3363..3785
                     /gene="queD"
                     /locus_tag="WGR04_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ0794977.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDDPVRGG
                     PKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            4054..4425
                     /locus_tag="WGR04_001444"
     CDS             4054..4425
                     /locus_tag="WGR04_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ0794978.1"
                     /translation="MKLLFIPLVLFVAAEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QNRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            4422..5060
                     /locus_tag="WGR04_001445"
     CDS             4422..5060
                     /locus_tag="WGR04_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ0794979.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGIPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVVAFCENMERK
                     IRAHHYYLSPCEQNGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(5395..5471)
                     /locus_tag="WGR04_001446"
     tRNA            complement(5395..5471)
                     /locus_tag="WGR04_001446"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5435..5437),aa:Pro,seq:cgg)
     gene            complement(5552..6637)
                     /gene="nagZ"
                     /locus_tag="WGR04_001447"
     CDS             complement(5552..6637)
                     /gene="nagZ"
                     /locus_tag="WGR04_001447"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ0794980.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAVGGVILFRRNFQ
                     NIVQLKELTAEIKALRTPGLIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDSESASA
                     AETAARQIGWVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHRNPEAVTRLALAL
                     QKGLEKGGMKSCGKHFPGHGFVEGDSHLVLPEDRRSLSELETADLIPFRGMSREGMAA
                     VMPAHVVYPQVDAKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     LSFEAGCDIILVCNRPDLVDELRDGFTIPDNPDLAGRWQYMANTLGSAAAQAVMQTTD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            complement(6693..8213)
                     /locus_tag="WGR04_001448"
     CDS             complement(6693..8213)
                     /locus_tag="WGR04_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ0794981.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWADGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNETHDETAVSDGSRGRVYALIIPVLVLIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTFGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVDPTLIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAGAAAAGYLVLGITKSALLGFGTTGIVLAVLIFMLKDKKRTIA"
     gene            complement(8414..9913)
                     /locus_tag="WGR04_001449"
     CDS             complement(8414..9913)
                     /locus_tag="WGR04_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ0794982.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFAQLVQSEGPAVVNIQAAPAPRTQNGSGNAENDSDPIADNDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEDLPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTSGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKASGALITKILPSSP
                     AERAGLQAGDIIRSLNGEEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHTGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEDGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            complement(10051..10680)
                     /gene="nth"
                     /locus_tag="WGR04_001450"
     CDS             complement(10051..10680)
                     /gene="nth"
                     /locus_tag="WGR04_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ0794983.1"
                     /translation="MNRQIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPIADTPQAMLDLGLDDVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKS"
     gene            complement(10726..11139)
                     /locus_tag="WGR04_001451"
     CDS             complement(10726..11139)
                     /locus_tag="WGR04_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0794984.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
BASE COUNT         2658 a         2990 c         3070 g         2464 t
ORIGIN      
        1 gctaatctag tacagcccct tgttttttcg atacggaacc ggatagagga aaaatcgaac
       61 attgcgcctg ccttgctatg attcacgaag aaatctccgc catgcctatg ggctatgaaa
      121 ccttgatcgg cgatatgggc agcgcactgt caggcggaca aaaacaacgc atcgtattgg
      181 cgcgggcctt atattgcgaa ccgaaaatcc tatttttaga tgcagcgacc agccatttgg
      241 atattgccaa tgaaaaagca gtcaatgcaa acttgaatgg cttgtctatc ataaaaatta
      301 tggcggcaca cagaaaggaa acggtggaat cagcagatag gaaaatgtct ttaggataaa
      361 aatacagttt caaaaatact caagattgct gctgtttttt cgcctgagcg tcaaactctg
      421 ccagcgtcat gttcaaagtc tgcaaacacg gcatcattac tgcatcgaca gcttggttca
      481 catgatccct ttccacaggc aacggacggt aaacgaagag cttgaagagt tcgttcaact
      541 caatcgaatc cgcccccgtt ttcaacaccc aaccctgtct gccggaatag atgtagccgt
      601 gccgcgccag cttttccaaa agctcgccca actcgtcgta gcccatattg atatgccgtc
      661 tgaactcctg aacaggcaag gctttgcctt ctttttgcgc cgcatccaga agcagcagga
      721 ttttcaacac gtcgtcaaac cgtccgcgcg agtcgaagcc cctgcggaac gcttctccct
      781 gccagtagga gagtgaagaa gtcagcaccg cgccgcccaa gaccagcgtc cacaacaggt
      841 tcagccacaa cagaaaaaac ggcacggcgg caaacgcgcc gtaaatcgag cggtagccgt
      901 cgaaattgcc catataccaa gtgaagaggg agcgcgcggt ttccagacaa aacgctgttg
      961 ccaaagcccc gacaaacgcc tgccgcgcgg gaacgaagcg gtttggcacg aagcggtaca
     1021 gcccccacag caaaagcgtc atgaaggtca gcgtcgccgc cgttcgcaac gcgcccgacc
     1081 actgcggcgc acctgaggca agcgcggcat cctgtaccga gccgaccata aaggaaatgc
     1141 ccacgcccaa agacagcggc ccgaacgtca gtaaagccca atagacgaga aactgcatca
     1201 tccacggacg ctgggaattg acccgccaga tgcggttgaa cgtattgtct atcgtccgaa
     1261 tcagcatcag cgaggtaacg accagcatca cgctgccgat tgccgtcagc cggttcgcct
     1321 gctcgcggaa cgcattgata tagtcgaaca ccatgtccgc gccctgcggc acaatggttt
     1381 ggttgacgaa ggagacgaac gaatccgacc agcggtcgaa cacggggaaa atcgaagcga
     1441 ccgccaccat cacggtcagc acggggacga gtgccagcag cgtcgtaaac gtcatgcttg
     1501 ccgccgcctg cggtacgcgt tcttcatcaa agcggcggac gacgaaccat gcaaacgcac
     1561 agattttatt gtctgccaaa ccttgcaaac gttgtaaaaa ggtcataatt tcttgcccgg
     1621 tcagtaagtt gggcattgat gcccgatgtt atagccaatt ttgccgtcag gaacaaatgc
     1681 ctgaactgcg gctgtttcag acggcatcgg aacaactgtt atgccgtctg aagaccgaac
     1741 cattttaacg gaatccgccc atgaacccaa atccccctca aaatcctcgt cctctactat
     1801 ttccaaaacg gcagcacccg caatcccgcc cgccgaatcg ctcgcggcat cgacagcgtt
     1861 gaaggttgcg aagccgtatt gcgcaccgtc cccaaagtct ccgccgtctg cgaagccgtc
     1921 aaaaaagata ttcccgacag cggctcccgt cctgaccgcc gaagaaaaca atatcgcctt
     1981 cgcacaaagc aaacgcttgg cggaactcgc catcaagtcg gcataagccg cgtgttcaga
     2041 cggcatggcg ttcagatgcc gtctgaacgc gtttgcctgt ataatccgcg tctttactat
     2101 ccaacttcgc ggttcgaaaa cctcccgcgt caccaaaact aggattcgat atgtcaaacc
     2161 aaaaagcctt ggtcatcttt tcgggcggtc aggattcgac cacctgcctg attcaggcaa
     2221 tccaaaccta cgggcgcgaa aacgtccaag ccattacttt ccaatacggg caacgccatg
     2281 ccgtcgagct ggaacgtgcc cgctggattg cgcaggattt gggcgtcaaa caaaccgtac
     2341 tcgacttgag cctgatgcgg cagattacgc acaatgccct gatggacgac accgccgcca
     2401 tcgaaactgc cgaaaacggc gttccgaata cctttgtaga cggccgcaac gcgcttttcc
     2461 tgctctatgc cgcgatttac gccaaagggc aggggatacg gcacatcatc gcgggcgtgt
     2521 gcgaaaccga cttctccggc tatcccgact gccgcgacgt gtttgtcaaa tcgatgaacg
     2581 ttacccttaa tttggcgatg gactatgatt ttcaaatcca cacgccgctg atgtatctga
     2641 ccaaggcgca aacttgggcg ttggcggacg aaatgggcgt gctggactat atccgcgagc
     2701 aaacccacac ctgctataac ggcatcgtcg gcggctgccg cgaatgcccg agctgtatct
     2761 tgcgcgaacg cgggctggcg gaatatctgg aaagtaaaaa ggccgtctga acacgcgcaa
     2821 accataagga atacgatatg cccaagctcc atatgtttta cctcggcggc aatgccggca
     2881 ggtcgaatat cgaagtgcac gacatccaat ttgctgtgtg cgacgactac cgtgaagccg
     2941 tccccgcact caaagccgcg tggttcggcg atgcggacaa aatccacatc gacggctggc
     3001 aggttgtcga atgggcggac ggttacgaca tcgccgtatc cgaaacgccc aaaacgaaaa
     3061 tgccgtctga aaacgccccg cgcttgtatt ttgccaatgt cggcggctat cgcgcagggc
     3121 agctcgccga agcgcacgct tttgggctgt tcgccgccgc cacgcctgcc gaagccaaac
     3181 aaaaagccct gcaaaccctg ttgaccgaca gctatgttca gcaacataaa gacaacttaa
     3241 aagacgtgga caacctgctt gcgctcgacc gcatcggcaa tttccatatc cgcctgaccc
     3301 cgaatccgca cggcaagcct gccgaaatcg gctttcaggg ctatttgccc atttgagaac
     3361 ccatgaaaat caccaaaatc ttcaccttcg actcctcgca tatgctcgac gggcatgacg
     3421 gcaaatgcca aaacctgcac gggcatacct acaaactcga aatcaccgtt tcagacgacc
     3481 ccgtcagagg cgggccgaaa gacggtatgg tgatggactt taccgacttg aaagccattg
     3541 tcaaacaaca cattaccgac cccttcgacc acgccttcat ctaccacggc ggcaacgggc
     3601 gcgaatctca aatcgccgca ctcttggaag gctggaacat gaaaaccctg cgcctgccct
     3661 gccgcaccac tgccgaaaat atggcggtcg aaatgtacga ccgtctgaaa aacgcggggc
     3721 tgaacgtgtg ccgcgtcaaa ttgtgggaaa cgccgacatc gtgtgcggag tatgaagggg
     3781 agtagggaat atcttgaacg tatcgatata gtaaattcca ataagacatg cccaaccgcg
     3841 tcattcccgc gcaggcggga atccagacct tgatttatca ggaatattta aaaattgcag
     3901 caattccaac tctctggatt cccgcctgcg cggaaaggac ggtttagagc gtccttattt
     3961 gaatttaccg taaaacggtt ttttctcctg tacggattcc ccgttttttc agacgacctt
     4021 ccatatcaaa tacacccatt aaaaggaata cccatgaaac tcctcttcat ccccctagtc
     4081 ctcttcgtcg ccgccgaaca tttctacatc gcctggcttg aaatgacgca gattcccagc
     4141 gaaaaagcgg cggaaacgtt caagctgcct tatgaattta tggaacaaaa tcgcgtgcag
     4201 accctgttcg gcaaccaagg gctgtataac ggctttctcg gcatcgggct ggtgtggtcg
     4261 cggtttgccg cgccggataa cgcggtgtac ggcgcgacgg tactgtttct cggcttcgtc
     4321 ctgattgccg ccgcgtgggg cgcgttctct tccggcaaca aaggcatact cgtcaaacaa
     4381 ggtttgcccg catttttggc agcggcggcg gtgttggcgg tatgaaaaaa atcaatgttg
     4441 cccccgaaaa tccccaatac cgcatcgtcg aaatctttga aagcctgcaa ggcgaaggct
     4501 ggaacacggg catacccgcc gttttcgtcc gcttgggcaa atgcaacctc gcctgcggct
     4561 ggtgtgatac cgattatttg acattcggca tgatgagcct gtccgacatc ttaggctgtc
     4621 tgaaaaccta tgccgcccgc aacatcatca tcaccggcgg cgaaccgacc atacagccgc
     4681 atctcgatat gctgctggac atgctcaagg cggaaggtta tttcctctgt atcgaaacca
     4741 acggactcaa tcccgcgccg ccgcaaatcg actatgtcgc caccagcccc aaagcctgct
     4801 atgccgccaa atacgaaaaa agctgtatcg aaacagccga cgaagtgcgg attgttgccg
     4861 acggcgatgt cgttgcgttc tgtgaaaata tggaacgcaa aatccgtgcg catcattact
     4921 acctttcgcc ctgcgaacaa aacggtgcaa tgaacatcta cgacaccatc cgccaaatcg
     4981 gtattctgaa cagccgcccc gacgcgccgg tgcattggca gttgagcgtg cagacgcaca
     5041 aatgggcggg aatagagtag tttaagcagt gtaactcaaa gggacggcgt acggttttac
     5101 cgatgtttga catacgggga aagtgtgccg cttctgcgtg gaaatgccgg catttccacc
     5161 gcccaatcag gacggagcct tactgaataa gatgctgccg ttgggtacaa gctcggcttc
     5221 ctaaattccg atggtctttt gaaccttgcc gatacccttt gtcggcgcac gcaaatggca
     5281 gggttgggga aaacgaaatg ccgtctgaaa cagaatgctg tttcagacgg catttttctg
     5341 ttgccgccaa aaggaaaaac cgccccggca atggataccg aggcggtttg gatttggtcg
     5401 gaatgagagg attcgaacct ccgacccctt cgtcccgaac gaagtgcgct accgggctgc
     5461 gctacattcc gaatgaattg gaacgggatt ataacgtaaa aagagcggca tgcctatacc
     5521 gttttgcctt tttgccgcgt gtcgggagga tttaaaaggc ctctccgact ttcacgccgc
     5581 ccgccgtgtc ttgcggcgag gccagaccgg caacaaaggc ttgcgccgct tggaaatccg
     5641 ttgtctgcat cacggcttgc gcggcggcac tgccgagcgt gttggccatg tattgccaac
     5701 gtccggccaa atccgggttg tcgggaatgg tgaaaccgtc gcgcaattca tcgaccaagt
     5761 cgggacggtt gcagaccaat atgatgtcgc aacctgcttc aaacgacagt cgggcgcgtt
     5821 ctttgatacc gccagccccg cacgcgcctt ccatggtcaa atcgtcagag aaaattacgc
     5881 ctttgaatcc gatatcgcgg cgcaaaattt gtttgagcca gatttcggaa aaccctgcgg
     5941 gcttggcatc cacttgcgga taaacgacgt gggcaggcat gaccgctgcc ataccttccc
     6001 ggctcatgcc gcggaagggg ataaggtcgg cggtttcgag ttcggacagg ctgcgcctgt
     6061 cttccggcaa gaccagatgg ctgtctcctt cgacaaatcc gtgtccggga aaatgtttgc
     6121 cgcatgattt catgccgcct ttttccaaac ctttttgaag ggcgagggca aggcgggtta
     6181 cggcttcggg gttacggtgg aaactgcggt tgccgatgac ggggcagttt ccccagtcca
     6241 aatccaagac gggagtgaag gacaagtcga tgccgcaggc ggaaagttcg gttgccaaaa
     6301 cccagcctat ctgccgggct gcggtttcgg cggcagatgc gctttcgctg tcccaaattt
     6361 gccctaagac gttcattgcg ggcaagcggg taaagccttc gataaaccgt tgaaccctgc
     6421 cgccttcgtg atcgacggca ataataagcc cgggtgtgcg cagggctttg atttcggcgg
     6481 taagttcttt gagctgtacg atattttgga agttgcggcg aaacaggatg actccgccga
     6541 cggcgggatc gagaaggcgt tgcttttcct cttcggtcag acggaaggcg gcaatgtcgg
     6601 ccattacggg gccgcgcgga atatggggaa cagtcatttc ggtttgctcc aaaaagcttc
     6661 agacggcata tgccgtctga aaagggggga aatcaggcga tggtgcgttt tttatctttc
     6721 aacatgaaaa tcagcaccgc caatacaatg ccggtcgtgc caaagcccaa cagcgcggat
     6781 ttggttatgc ccaaaacaag ataaccggcc gctgctgcac ccgctacggt cagggcatag
     6841 ggcagttgcg aggtaacgtg gtcgatatgg ttgcagcgcg cgccggtgga agacaggatg
     6901 gtcgtgtcgg agatgggcga acagtggtcg ccgcataccg ccccagccat tactgcggac
     6961 atgcacggga taatcagcgt gggatcgact ttgaccgcca ttgccgcggc aatcggcagc
     7021 atgatgccga atgttcccca acttgtgccg gtggcaaacg ccattacgct ggcgagcagg
     7081 aagaggatga cgggcaggaa gccgggatgg atgttgcccg caaccagtgt ggagaggtaa
     7141 tcgccggtgt gcatttcgcc gacaaccgta ctgatgagcc aagcgaggat taaaatggcg
     7201 attgcgccga acatagattt cgcaccctgc caaacggctt tgggataatc ggcggtttta
     7261 atcgtgccga acgtgcagag gacgacggca agcacgccgc aagtgccgcc gaataccagc
     7321 gaagtgttta cgtccgtatt ttcaaatgcc cccaaaatgc tgaaggtttc gcttgcctgt
     7381 gcgccggtat agatcatggc ggatacggtg gaggcgatta agaccaaaac gggaataatc
     7441 aatgcgtaaa ccctgcccct gctgccgtct gaaacggcgg tttcatcatg ggtttcgttc
     7501 aaagcggctt gttcgaaacg tgccatcgag ccgatgtcga aggaaaacca tgcgacgacg
     7561 aacaccataa tcagggcaaa cagcgcgtaa tagttcatca ggctcatggc gacaaatgtc
     7621 cccatcggcg tgtattcggt aattttgtag gtaacgagca atccggcaag tgtcgcgata
     7681 atcgacgcgc cccaacttga aacgggcatc agcacgcaca taggcgcggc agtggagtcg
     7741 aggatgtagg cgagtttggc gcgggaaact ttaaacttgt cggtaacggg gcgggcaatc
     7801 gcaccgacgg cgagactatg gaaatagtcg tcgatgaagg taacgaatac gaggcaggcg
     7861 gtcagcattt ttgcgccgcg tcggttttta atgtgccgtt ttgcccagtc ggcaaacgcc
     7921 tgattgctgc cggagtaggt cagcagggaa gtgaaaatgc ccaaaagtat caggaaaact
     7981 aagatttttg gtttgcccag cgaccaatcg ccgtctgccc aagccaagcc gacgaccatg
     8041 tctttcaggt gtgtcagacc gtcgacgggg ttgccgccga ccaaaaaggc aacgccgacc
     8101 aaaattccga tgcctaaaga cagcagtacg cggcgggtaa tgacggcaag tgccagtgcc
     8161 aaaaagggtg gcacaaccga gaaaaatgaa tgtgaatagt cgatcagctg catggtttat
     8221 gggggtgtta agcgtccgga tgggagggta tctgtccgcc tccggtttgg gttttgttgg
     8281 caaaatgggc ggaaatattt ttgtcgtaaa aaatatttgt ttaaaatcaa acaaccgatt
     8341 ttttaaaatg cccattaatc gatattgatg ggcattttat catttaaaaa atattttggt
     8401 taaattatgt gtgttattgc aggtttaatg cgataaacag cgtgttgcca cggcgcatga
     8461 ccagcagggg gacgtttttg cccgccttgt ccatagcttt gcggaaaccg tcttcgtcat
     8521 tgacggggac ttgcccgacg gcaagaattt cgtcgccgcg cctcaagcct gcgcgttctg
     8581 ccgcgtcgga aacccgtacg acgacgaggt gtccgccgct gctgtcggta tgtgtctgaa
     8641 gggtaatgcc tgcggattca accgagaacg taccggattg ctgttcggtg tagggggctt
     8701 catctgtttt ggatgatgcg ccggtatgct cggcagcgtt gcccagcttg actttgattg
     8761 taatttcttc gcctttgcgc catacgccga ggctgacttc ttttcctggt gtaatggcac
     8821 cgaccataac gggaaggtct ccggaagaac gtatttcttc tccgttgaga ctgcgtatga
     8881 tgtcgcctgc ctgcaagcct gcacgttctg cggggctgct gggaaggatt ttggtaatca
     8941 atgcgccgct ggctttatcc agaccgaagg actgtgccaa accgtaggat acttcttgaa
     9001 taatcacgcc cagttgcccg cgttggactt tgccggtgtt tttcagctgt tcggcaacat
     9061 tcatggcaac gtcaatcggg atggcaaagg aaatgcccat aaatccgccg ctgcggctgt
     9121 atatttgcga gttgatgccg acgacctgtc cttttaagtt gaacagcgga ccgccggagt
     9181 tgcccggatt gatggcgaca tcggtttgga tgaagggggt gtagctttcg ttgggcaggc
     9241 ttctgccttt ggcggacaca atgccggagg taacgctgtt gtcgaagccg aagggtgcgc
     9301 cgatggcggc tacccattcg ccgggtttca aatctttggg attgccgatt ttgacgacag
     9361 ggaggtcttc cgttgcgtct atttttaaaa gcgccacgtc ggattggaca tccgagccga
     9421 tgagtttggc ggtatattcg cgcttgtcgt tgagcaggac tttgatactg cccatgccgg
     9481 taacgacgtg cgtattggtc aggatgtagc cgtctttgct gatgatgaag ccggagccga
     9541 agttcaatcc accgtcatct gcttcttctt gggggatttc gggcatattc gggacgaggc
     9601 gtttgaaaaa ttcgtagaat gggtcgttgt cggcaatcgg gtcggaatcg ttttcggcat
     9661 tgccgctgcc gttttgggtg cgcggggcgg gggctgcctg aatattgacg actgccggac
     9721 cttcactttg aaccagttgg gcaaagtcgg gcagcagcat actgacgctg ccgtcgtctt
     9781 tggtgtgttc gatgcgttct acgaaggatg cttctttttt gtccgcaccg aaaaagctgc
     9841 ctgccttgtc gcagcctgcc agcgaggcgg cacacagtgc tgccaaagcg aggtattggt
     9901 attttttgaa cacgtttcgt cctttgtttc gtaccgatat tgacttcaat gccgtctgaa
     9961 acgcacgttg tcggtttcag acggcatgga gtgaaatttt acaacgtccg tccgaatttt
    10021 caagcatttc attttgaagg gcggcggcgg tcaggatttg gcgggatatt cgcacaaatc
    10081 gttgatgatg caggtttggc attgcggttt gagtgccttg caggtgtagc gtccgtgcaa
    10141 aatcagccag tggtgcgcgt ccatcaggaa ctctttagga atgaagcgca tcagtttgtc
    10201 ttcgacttcg cgcacatctt tcccgggagc gattttggtt cggttggaca cgcggaaaat
    10261 atgcgtatcg accgccataa cggggtgtcc gaacgctgtg ttcaatacca cgtttgccgt
    10321 tttgcgcccc acgccaggca atgattccaa agcctcgcgg tcttccggca cttcgccgtt
    10381 gtatttttcc agcaggatgc ggcaggtctg catgatgtgt ttggacttgg ttttatacaa
    10441 cccgatggtt ttcgtgtatt ccatcacgtc gtccaagccc aaatccagca tcgcttgcgg
    10501 cgtatcggca atcggaaaca gcttcgccgt cgccttgttt acgccgacat cggtcgcctg
    10561 cgccgacagt aaaacggcga ttaaaagctc gaaaggggag ttgaaattca gctcggtggt
    10621 cggatggggg ttggcggcgc ggaaacgctc gaagatttct tggcggattt gtctgttcat
    10681 ttttttgtgc agtcggtttg tgcggtcggc attataacgc acggttcagg cggcgtaata
    10741 ttgcattccc cacagaatga aggcgtaacg cgccgttttg ccgataacca gcatcagccc
    10801 gcttgtccac ggattcaacc gcagccagcc ggcggcaagc ggcagtgcgt cgccgacgac
    10861 gggcagccag gcaaacgcaa gcagccaaat accgaaacgc cgcatcagat tcagtgtttt
    10921 ttcagacggc atttttcggg agggcagcaa acgccccatc caataggaaa ccatactgcc
    10981 caatccgttg gcaaggccgg cgcacagcaa cgcgccgtat gcgtgttcgg gaaagcggtg
    11041 gacgaacagg gcaaaggcgg cttcggatgt gccgggcagg agggtggcgg aagtgaaggc
    11101 ggaaaaggcg agggcggcgt aggtgtagga gggtatcatt gcaaacagtc tcaaacaggc
    11161 aaaaatcggc gacggattgt ac
//
LOCUS       DAWFJR010000061        11167 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWFJR010000061 DAWFJR010000000
VERSION     DAWFJR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11167
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.061"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            141..1538
                     /gene="aspA"
                     /locus_tag="WGR04_001452"
     CDS             141..1538
                     /gene="aspA"
                     /locus_tag="WGR04_001452"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ0794985.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVISNLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLAVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTTGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLAEKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1735..2610)
                     /locus_tag="WGR04_001453"
     CDS             complement(1735..2610)
                     /locus_tag="WGR04_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0794986.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRAESVVGGILLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWAEVKALFGI"
     gene            complement(2612..3334)
                     /locus_tag="WGR04_001454"
     CDS             complement(2612..3334)
                     /locus_tag="WGR04_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ0794987.1"
                     /translation="MDKDLYAVLGVSPQAGANEIKRAYRKLAMKYHPDRNPGNPQAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSRHAYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYATHTEYLIKTFWRTFWLYLLGALTALLGIGVLIIIATNVWYF
                     YRIIVGFIRFNSGRAVAPEKWI"
     gene            complement(3356..3835)
                     /gene="ybaK"
                     /locus_tag="WGR04_001455"
     CDS             complement(3356..3835)
                     /gene="ybaK"
                     /locus_tag="WGR04_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ0794988.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLDIIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3910..4272
                     /locus_tag="WGR04_001456"
     CDS             3910..4272
                     /locus_tag="WGR04_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0794989.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAMKKDAATA"
     gene            4307..5170
                     /locus_tag="WGR04_001457"
     CDS             4307..5170
                     /locus_tag="WGR04_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ0794990.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKVVFEILEDYAVDAEL
                     EASAEPLAAQEPNLAFAAQQDSDGICSIALPHGGILRIAPETALPPYDAAAESAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAAEAGILLTADGEEAGIVLDSVKDSENFTALAVIKFSAAQKTLSAPNGGSFKA
                     VHLFFKTENAE"
     gene            5263..6222
                     /gene="ttcA"
                     /locus_tag="WGR04_001458"
     CDS             5263..6222
                     /gene="ttcA"
                     /locus_tag="WGR04_001458"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ0794991.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIELGDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(6316..9255)
                     /locus_tag="WGR04_001459"
     CDS             complement(6316..9255)
                     /locus_tag="WGR04_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HEZ0794992.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRAADENPELLFAA
                     KQYANNILKVQTQNGIDGRFPDGSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTRQFLQSKALAEHFEQDFGGDYEGVRLNTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSPSMTDTGDKALKDLFDNPVDALAAVRPFV
                     IVDEPHKFPTRDSAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAKIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFTK
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGIRTAVNEAYINNGLMLSRRIDSFEDEDFIR
                     FNTMTYREFLEKLAQTAKIRMQTLHQAFYCIRNELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFDEIF
                     YDSDIEHENVANNQIEGVTVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAENLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(9268..11073)
                     /locus_tag="WGR04_001460"
     CDS             complement(9268..11073)
                     /locus_tag="WGR04_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0794993.1"
                     /translation="MFPACFDADGNFLIDRLQAEIAPQTDIGREFYEMNWLGKSYARL
                     LRNLPPETLISEDKTHNAKPENAGSQNLLIRGDNLEVLKHLKNAYTNSVKMIYIDPPY
                     NTGSDGFVYQDDRKFTPAELARLANIDEDEAARILDFTDKGSNSHSAWLTFMYPRLYV
                     ARELLKDDGVIFISIDDNEAAQLKLLCDEVFGEGNFVEQIIWEKKFSPQNDAKYFSEN
                     HDYLICYAKNITELEIKLLPRTEEMNARYKNYDNDPRGAWTSDNLLRKDVQQSGIYTI
                     TTPNGTQYNPPSGRSWRVSQTKFTEMVADNRIWFGEDGGNVPRIKRFLSEVQDGTKAI
                     SIWKFNEVGHNQEASQELRKLFDGDSYFDTPKPIRLIVQTLRLTTNSDSLILDFFAGS
                     GTTAHAVMQLNAEEQNGSRRYICVQLPEKTAEKSEAHKAGYPTIFDITKARIEKAAAK
                     IRAEYPDYTGDSGFKIFQTADNFRQHPDKDFSPEQPDLPLNDELSEEQLQTLLTTWTL
                     YDGAALTTPVEPVGLGDYTAYLCEKRLYLMNAGFTSADLLAFIRKLDDDADFNPNRVI
                     VFGSNMASAMQHELDQAVRGYTNKKEIELNVIIRV"
BASE COUNT         2660 a         2998 c         2682 g         2827 t
ORIGIN      
        1 ccgaaaacaa cgtttcccgt tttcagacgg catacgatat ggacaatttc atccggccgg
       61 tatagtgttc gtcctgcggc ggcatctttc cgatggcgca ttttttcaat attaattatc
      121 cgacttacaa aggtaaaaac atgactgtcc gtatcgaaca cgatttattg ggcgaccgcg
      181 agattcccgc cgaagtgtat tggggcatcc acactctgcg cgccattgaa aactttaaaa
      241 tctccacaca aaaaatttcc gacgtgccgc agtttgtccg cagtatggtg atggtgaaaa
      301 aagcaaccgc gcaggcaaac ggcgaattgg gtgcagtaaa acccgaaatc gccgccgcca
      361 ttgaaaaggc ttgcgacgaa gttctgctga acaaccgctg cctcgaccaa ttcccctccg
      421 acgtgtatca gggcggtgcg ggaacttcgg tcaatatgaa caccaacgaa gtcatctcca
      481 acctcgcatt ggaagccttg ggatacgaaa aaggccgcta cgacatcgtc aatccgatgg
      541 accatgtcaa cgccagccaa tcgaccaacg atgcctatcc cacgggcttc cgccttgccg
      601 tgtattacag catcggcgaa ttgctcgaca aactggccgt cctgaaaaac gcctttgccg
      661 ccaaagccga agagtttaaa gacgttttga aaatgggccg cacccagctt caagatgccg
      721 taccgatgac tacaggacaa gaattccaat ctttccaagt gttgttggaa gaagaaatcc
      781 tcaacctcga ccgcacgcgc caactgctgt tggaagtcaa tttgggcgca acggcaatcg
      841 gcacgggcgt gaacacgccc aaaggctacg ccgaactggt ggtcaaaaaa ctctccgaag
      901 tcagcggctt gccttgcaaa ctgaccgaaa acctgattga ggcgacctcc gactgcggcg
      961 catatgtgat ggtacacggc gcattgaagc gcacggcggt caaactctcc aaaatctgca
     1021 acgacttgcg cctgctctct tccggcccgc gtgccggctt gaaagaaatc aacctgcccg
     1081 aattacaggc cggttcttcc attatgcctg ccaaagtcaa tcccgtgatt cccgaagtgg
     1141 tcaaccaagt ctgcttcaaa gtcatcggca acgacaccac catcactttc gccgccgaag
     1201 caggtcagtt gcaattaaac gtaatggagc cggtcatcgc ccaatgtatg tttgaaacga
     1261 tttccctctt gggcaatgct gcggtcaacc tcgccgaaaa atgcgtcaaa ggcattacgg
     1321 tcaaccgcga aatctgcgag cgttacgttt tcaactccat cggtttggtc acttatctga
     1381 atccgtacat cggccaccac aacggcgact tggtcggcaa aatctgcgcc cagaccggca
     1441 aaggcgtacg cgaggttgtt ttggaacgcg gcctgttgag cgaagaagaa ctcaaccgca
     1501 tcctctctcc tgaaaacctg atgaaccccc atctgtaacg ttgcaggcaa atgccgtctg
     1561 aagcagggat gctgcttcag acggcatttt cggcattcaa ggctgtttta gccgccgccc
     1621 gattccggcg gtttgtcgga aaaagccagt tatttccgtt cgggaaaaat ccagtttatt
     1681 gacaaaaaaa caattactta aaacgcgttt gcgcggtatg cggacacggc gggattatat
     1741 gccgaacagt gccttgacct ccgcccaata aaacaggcag gcgatggaac acagtgccaa
     1801 tacgattccc gccgtattga ttgtgttgat tttttcctta aaaaacagtg cgcccgaaag
     1861 cgtacccaaa acaatcacac cgatattcat accggcaaaa accaaggtcg gattgtcctt
     1921 catcatttgg tgcgcggtga tgtaggttac gatattcata aaattcaaac cgcccaagag
     1981 tatgccgccg accacactct ctgcgcgcca tctgaccgat ttggcaaaca ggcaggcaaa
     2041 catcagcaca cccgccagca caaatgcaac cagcaggttg cccgcaaatg ccgttccgct
     2101 tttggcaagc tgtttgaaca ggatatcgat aatgccgtaa cctgcccaca cgcccagcag
     2161 caatgccgcc tgccgccacg cgcttcctga ttttttgcca ccgctgtgtt tccaaagcag
     2221 gcagaacagt gcggcaaagg cgaggcacag cccgattagt ttgccttcgc tgagtttttc
     2281 gccaaacagc gttaaagcgg caacaatcgg caaaaacagc gacaaacgct gcgccgcgtc
     2341 ggatttgacg ataccggcgg attctacgga tttgcccatt atcacgaata cggacggcag
     2401 cagcacgcct aatgcggcaa acagcggcca cgtcggcaaa aatgcgccga tattgccgac
     2461 atccggcttc aataccagca gggtcagtat gaccgcgacc acataattga cggcgaccgc
     2521 ctgcgcgatg tcgatttttt tcttccttgc cattttcagc aaaatggaaa ccgacacgct
     2581 gaacacgatg ctgattaaca ggtaagccat actatatcca tttctcgggt gcaaccgccc
     2641 tgccgctgtt gaagcggata aagccgacga tgatgcggta gaaataccag acgttcgttg
     2701 caataataat cagcacgccg atacccaaaa gggcagtcag cgcgcccaaa agataaagcc
     2761 aaaatgtgcg ccaaaaggtt ttaatcaggt attcggtatg tgtggcatag acaatgctgt
     2821 ccaaactgtt ccgtttcata taggcgagaa tcacgccgac tatgggcata taggcgaaaa
     2881 tcacgccaac tatgggcatg aacagcatga ttgcacccaa accgaacagg atgtaggcgg
     2941 cgaggacata gttgcgcccg cttccgccgc cggacggttc gtaagcatgg cgcgaacgtg
     3001 aggcctgccg ttcaaacgcc tgtctgaacg cctgttcgcg gcgcatctgt tcgcggtaaa
     3061 actgctccct gcgcgcctgt tcgcggcgga atgcctcttc ctgccgcccg cgttcctcat
     3121 gccgtctgaa ggacgcgtcg tattgcatcc gtttcgacag gtcggaaagc gtatcgtaag
     3181 cccgttggat ttctttgaac ttttcttccg cctgcggatt gcccggattg cggtcgggat
     3241 ggtatttcat cgccagcttg cggtaggcgc gtttgatttc gttcgcaccc gcctgcggcg
     3301 acacgcccaa tacggcatac aaatctttgt ccatattgct ccttatactt cccggtcaaa
     3361 ccgccgcctg tattgttttc gggttcaaaa tatttaaatc tccgggacgg atgccgataa
     3421 tgaacccgcg ttttccgccg ttgatataga tgatgtccaa atccatcacc gactgttcga
     3481 cataaatatc caattttgtc cggatgccga acggcgttgt gccgccgacc agatagcccg
     3541 tccacttgtt tgcctgtgct ggcgtggcgg gttcgatgtg tttcgcaccc aaatgccgcg
     3601 ccagattgcg ggttgaaatc tgcttgtcgc cgtgcatcaa gacaatcagc cccttaccgt
     3661 tttcatcttg caaaacaatg gttttaatga ccaagtgttc gtctttgccg aacagtcggg
     3721 caaactgcgc cgtgccgccg tgttcctcat aggcataggt aaaaggttca aattcgatgc
     3781 cgttttcacg caaaacgcgc acggcaggtg ttatcggata agtgtgttta ctcatttttc
     3841 gtcatgcaaa acatggaaat cggtcaagcg caatgttacc atatatcccc tttatcgaag
     3901 gaaaccgata tggatatccg atatttcggc acaacgcccc gctattccga agccgtcggc
     3961 gcaaacggct tgattttcct ctccggcatg gttcccgaaa acggtgaaac ggctgccgaa
     4021 cagactgccg acgtgcttgc ccaaatcgac cgctggctgg cggaatgcgg ctcggacaaa
     4081 gcgcacgttt tggatgcggt catctatctg cgcgatatgg gcgactatgc ggaaatgaac
     4141 ggcgtgtggg acgcttgggt tgccgccggc agaacgcccg cccgcgcctg cgtggaagct
     4201 cggctcgccc gtccggagtg gcgcgtcgaa atcaaaataa ccgccatgaa gaaagatgcg
     4261 gcaaccgcat aaacagattt cctttatatt tcagagtacg caaatgatga aaaccttact
     4321 gccctttttc ggcgtggtgc gcgtcagcgg cgaagaccgc caaaccttcc tgcacggaca
     4381 gctttccaac gacatcaata atcttcagac cgggcaggcg tgttatgcga cttacaacac
     4441 gcccaaaggc cgcgtcatcg ccaatatgat tgtcgtcaac cgaggcggcg atttgctgct
     4501 gattatggcg caagacctgc ttgaagcaac cgtcaaacgc ctgcggatgt ttgtgttgcg
     4561 tgccaaagtc gtttttgaaa tccttgaaga ttacgccgtc gacgcggaat tggaagcatc
     4621 cgccgaaccg cttgccgcgc aagaacccaa ccttgccttt gccgcacaac aagattcaga
     4681 cggcatctgc tctatcgctc tgcctcatgg cggcatcctt cgcatcgcac ccgaaaccgc
     4741 cctgccccct tatgatgccg ctgccgaaag cgcgtggcgg ctgcacgaaa tccgcagcgg
     4801 ttatccgtgg atatgcgcgg ctaccaaaga aaccgccgtc gcccaaatgc tcaaccagca
     4861 catcatcggc ggcgtacact tcaaaaaagg ctgctatccg ggacaggaaa tcattgcccg
     4921 tgcccagtat cgggggcagg tcaaacgcgg cttggcggta ttgtcgggaa attcggcagc
     4981 cgaagcgggc atcctgctga cggcggacgg cgaagaggcc ggcatcgtac tcgacagcgt
     5041 taaagattcg gaaaacttta ccgcccttgc cgtgattaaa ttctccgccg cacaaaaaac
     5101 actgtccgcc ccaaacggcg gcagcttcaa agcggttcat ctcttcttta aaaccgaaaa
     5161 tgcagaataa gccgccgatg ccgtctgaag cccttcagac ggcattgccg ccccgtcaat
     5221 cggttacaat gccgcctttc caattttcaa acccgaaccg ttatgtctaa aaaaaccaag
     5281 caagaactcg aaaacaacaa actcagcaaa cgcctgcgcc acgccgtcgg cgacgcgatt
     5341 aacgatttca acatgatcga gctgggcgac aaaatcatgg tctgcctctc cggcggcaaa
     5401 gacagctatg ccctgttaga catcctgcgc cagctccaag ccagcgcgcc gattgatttc
     5461 caactggtcg ccgtcaatct cgaccaaaag cagccgggct tccccgaaga agtattgccg
     5521 acctatctcg aaagcatcgg cgttccctac aaaatcgttg aagaagacac ctactccacc
     5581 gtcaaacgcg tattagacga aggcaaaacg acttgttcgc tgtgcagccg cctgcgccgc
     5641 ggcatcctct accgcactgc aaaagaattg ggctgcacca aaatcgcctt gggacaccac
     5701 cgcgacgaca tcctcgccac cttgttttta aatatgtttt acggcggcaa actcaaagcc
     5761 atgccgccca agctggtgag cgacaacggc gaacacatcg tcatccgtcc gctggcgtat
     5821 gtaaaagaaa aagacttaat caaatacgcc gaattaaaac aattccccat catcccgtgc
     5881 aacctctgcg gctcgcaacc caacctgcaa cgccaagtca tcggcgatat gctgcgcgat
     5941 tgggacaaac gcttccccgg ccgcatcgaa tcgatgttct ccgccctgca aaacgtcgtc
     6001 ccttcccacc ttgccgatac cgaactcttt gactttgtcg gattggaacg cggtcaaacc
     6061 ctgaaacacg gcggcgattt ggcgtttgac agcgaaaaaa tgcccgaacg tttttcagac
     6121 ggcagcgaag aagatgagag cgaaatcaaa atcgagccgc aaaaagccga acgcaaagtc
     6181 atcaacattc tggctaacaa gccgaaaacc tgcggcgcgt aaccccccgc cgtcaaaaat
     6241 gccgtctgaa gcccgtttgg cgtttcagac ggcattttaa aatcccgaag cagacggctt
     6301 ccaaacattt gtttctcagt gtccgttttc agaatgcccg cctgcgggca tattttgccc
     6361 gatcagttcg gctatcctct cgccgtcaaa ctgcgttttg aacaccacct tgatttcttt
     6421 ggaaatctcg gcaaacagct tttcggcatg tttgattttc cgctcttcgc tttttcgcaa
     6481 attttccgcc ccgtcagtcc ctttggcttc aatcacaaag ttcaggatct cgccgctttt
     6541 ggttttcacg atataggcaa aatcgggcga atacgtgccg ccgccggcaa cagggatttt
     6601 gatggagttt ctcggtattt tggtaaatac ggttacgcct tcaatttggt tgttggcgac
     6661 attttcatgt tctatatccg aatcgtagaa aatctcgtcg aagagatagc cggcggcagg
     6721 ccggtgctcc gtatcttcaa atctgcccaa atctgctttt ttcaccgcgc gcggtttgcc
     6781 gtctttattg gtaaatttgg tcggatggat tttgctgccg acaagccggt aatccagttc
     6841 gaatttatgg aaggaatgat gaagcaaaaa ccggttgaag ccgtttttga tttgggcgat
     6901 ggtttgcata ttcaaaaaat cgccaatgtt cagttcgttg cggatgcagt aaaacgcctg
     6961 atgcaaagtc tgcatacgga tttttgccgt ttgtgccagt ttttccagaa actctcggta
     7021 agtcattgtg ttgaaacgga taaaatcttc atcttcaaaa ctgtctatgc ggcgggaaag
     7081 cataagcccg ttgttgatat aagcttcgtt taccgccgtg cgtatgcctg cctgcgggaa
     7141 tttggcggcg ttttcacgca aataggcggt aaataaatcg acaaattcgg cttcatcctt
     7201 gattttgtac tgcaaaacgg ctttatggtg aatcagctcc cacaaggcct tgagttcttc
     7261 atatttgcct tcgcgcatga tgatggtgtc tttgccctcg tctttggcgt tgctgacttt
     7321 gcctttgtcc aaacctttgg ggaaggcttc gggataggcg gcttttaatt tgtcatagcc
     7381 gtctttggta aagttttcat tgtcgtcaat gatgccgtct gaaaacagtt ggtttaccaa
     7441 taccagcggt ttgatatcgg ggtatttttg caatattttt tgtttcagct cttcggtaaa
     7501 ctttttggag atttcttcct gaaaagaatt atcgttgatt tcgccgacaa gctgcttcac
     7561 aaagtctttt tcgctgctat cgacaaaata attcagtttg tacggcacat cgcgcacccg
     7621 cgccatcagc tcgtttaccg gcaggcgcag gccgcgtccg acttcttgca gcttggaagt
     7681 cgtgctgccg ctggaacgca gtttgcaaat ctggaaaacg ttgggattgt cccagccttc
     7741 gcgcagcgtc catttggaaa aaataaagcg gcgcgggttg tccaaagaca acagtttttc
     7801 cttatcgtgc aggatttcat tgatttcctg ctcgattcta tcgtcgctgt ctgtattgtc
     7861 tttggaaaaa taaccgccgt ggcaggcgga tacatcgtcc aacgtctttt gcaggtaatc
     7921 gcggtaaaac gggtcgcttt ccgttttcag gcggcgtgcc gcttccgcgc gaatccagct
     7981 ttcaaattta tctttcaggc tgcctgaaag ctcgttgccg ctgcggtagc ccgcaatatc
     8041 atcaataaaa aacagcgtca gcggcttgat tttgggctgt ggcgcgcgtt ctgccaaaag
     8101 cgcgcgttcc agcttgaaat gttcggcaac cgcccgctgc atcatcgcat cctgcaccgt
     8161 ttgcgaatag gaataagggt tgatgacggc ccccgttttc aactccaagc cgttgcttaa
     8221 caccaccacg gttttattca ttttgtcgat tttcaaatcc gaaatagcgg gatggatttt
     8281 cgccaaatct tcgccttttg ccagtttgaa cgtctgcttt ttgtcctttt cgtttaattc
     8341 aaatttcgct tctttgccgt ccgacgacac cagttttacc gctgcgtcca tgccgccctg
     8401 catttcttcc tgaaacacgc gcacgccttt gaccagcccg tcgttaaacg cgtccaccgc
     8461 cgtcagacgg taaagcaggt tgtaatattc atcgttaaat gtcgcaccgt agcgcaaaat
     8521 atattgcggt tttaagcgtt tgatattgcc ccacgttttc gcgctatctc gggtcgggaa
     8581 tttatgcggt tcgtccacaa tcacaaacgg gcgcacggca gccaatgcat caacgggatt
     8641 gtcaaacaaa tccttcaatg ccttgtcgcc cgtatcggtc atggacggcg aattaaccat
     8701 gcccgcgtta atcagcagca catgaatttc ctttttgttt tccgctttga caaattgctc
     8761 gatcgttatg ggcgcattgg actttttgcc cttattcttt ttcgcgcttt ccaccacata
     8821 agtgttcagg cgtacgcctt cataatcgcc gccgaaatcc tgttcaaaat gctccgccaa
     8881 agccttgctt tgcaaaaact gccgcgttcc cgccttaatg gacaaagtcg gcacgaccac
     8941 gataaatttg aacacgccca gccaacggtg cagctcgaac atggtttgtg tgtaggtata
     9001 ggttttgccc gtgcccgttt ccatggaaat atcaaggata ttttggtcgt ccgaaccgtc
     9061 ggggaatcgg ccgtctatgc cgttttgggt ttgcactttc aggatattgt ttgcgtattg
     9121 ttttgcagca aacaaaagtt cgggattttc gtctgccgcc cgatatttgg gcgttgcccc
     9181 gtcaaacacg cccaaaaccg ccgaaaccgc ccgcatttgg tgcggctggt ttttctcgta
     9241 attaaaaccg ctcatgaatt tcctccgtca aaccctgata atcacgttta actcaatctc
     9301 ttttttattg gtataaccgc gaaccgcctg gtcaagttcg tgctgcatgg cgcttgccat
     9361 attgctgccg aacacaatca cgcggttggg attgaaatcc gcatcgtcgt ccagcttgcg
     9421 gataaacgcc aacaaatcgg cggaagtaaa accggcattc atcaggtaca gccgtttttc
     9481 gcacagatac gccgtgtaat cccctaaccc cacaggctca accggcgtag ttagtgccgc
     9541 cccgtcatac agcgtccagg tggtcagaag cgtttgcagc tgttcttcgc ttaattcatc
     9601 gttaagcggc aaatccggtt gttcgggcga aaaatccttg tccggatgct gcctgaaatt
     9661 gtctgccgtt tgaaagattt tgaagcccga atcgcccgtg taatcgggat attcggcgcg
     9721 gattttggcg gcggcttttt ctatgcgggc tttggtgatg tcgaagatgg ttgggtagcc
     9781 cgctttatgg gcttcggatt tttcagcggt tttttcggga agctgcacgc agatatagcg
     9841 tcggctgccg ttttgttctt cggcgttaag ctgcatcacg gcatgggcgg ttgtgccgga
     9901 accggcgaag aagtctagga ttaggctgtc tgaatttgtg gttaatcgca atgtttgtac
     9961 aatcaaacga attggcttcg gtgtatcaaa ataactatcg ccatcaaata atttacgcaa
    10021 ttcttggctt gcttcttgat tgtgtccaac ttcattaaat ttccaaattg aaatggcttt
    10081 cgtaccatct tgaacttcgc ttaaaaagcg tttgatacga ggaacatttc caccatcttc
    10141 accaaaccaa atgcgattat cggcaaccat ttcggtaaat ttagtttgcg aaacacgcca
    10201 gcttctaccg ctgggtggat tgtattgtgt accgtttggc gttgtaattg tataaattcc
    10261 gctttgttgc acatctttac gcaataaatt atcagaagtc caagcaccgc gtgggtcatt
    10321 atcataattt ttataccgtg cattcatttc ttctgttcgc ggtaataatt tgatttccaa
    10381 ttcagtaata ttttttgcgt aacaaattaa ataatcgtga ttttctgaaa aatattttgc
    10441 atcattttgg gggctgaatt tcttttccca aataatctgt tctacaaaat tcccttcccc
    10501 aaacacttca tcacacaaca acttcaactg cgccgcttcg ttatcgtcaa tcgagataaa
    10561 aatcacgccg tcgtccttta acagttcgcg ggcgacatac aggcgcggat acataaaggt
    10621 gagccatgcg ctgtgcgagt ttgagccttt gtcggtgaaa tctaaaatcc gcgcggcttc
    10681 gtcttcgtca atattcgcca ggcgggcaag ttcggctggg gtgaatttgc ggtcgtcctg
    10741 atagacaaag ccgtccgagc cagtgttgta gggcgggtcg atgtaaatca tcttcacgct
    10801 gtttgtatag gcgtttttta agtgtttcaa cacttctaga ttgtcgccac gaatcagcag
    10861 gttttggctg cctgcatttt cgggcttggc gttgtgcgtc ttatcttcac ttatcagggt
    10921 ttcgggcggc aggttgcgaa gcaggcgggc atatgatttg cccagccagt tcatttcgta
    10981 aaattcgcgt ccgatgtcgg tctgtggcgc aatttcggct tgtaatctgt cgataaggaa
    11041 atttccgtct gcgtcaaaac aggcgggaaa cagttttttg agctgttcga gttgggtaga
    11101 gttggcggta atgccgtctg aagtgtagat tgcctcggtg ttcgccccgg ctgtgtcgct
    11161 tagatca
//
LOCUS       DAWFJR010000062        10764 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWFJR010000062 DAWFJR010000000
VERSION     DAWFJR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10764
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.062"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            154..1038
                     /locus_tag="WGR04_001461"
     CDS             154..1038
                     /locus_tag="WGR04_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ0794994.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRHLESGIREKISSHIARGKVECKIQIQDAENGSQSLELNRDLVGQLAEINKDLR
                     KHHDLAKLGVADILRFPGVLASQRENTEELAKSITELTEKALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIDALSELFPTLLETHKEKIRARLVEAVGDINNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(1122..1637)
                     /locus_tag="WGR04_001462"
     CDS             complement(1122..1637)
                     /locus_tag="WGR04_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0794995.1"
                     /translation="MMILKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGAWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(1712..2131)
                     /locus_tag="WGR04_001463"
     CDS             complement(1712..2131)
                     /locus_tag="WGR04_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794996.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAIIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(2252..3250)
                     /locus_tag="WGR04_001464"
     CDS             complement(2252..3250)
                     /locus_tag="WGR04_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ0794997.1"
                     /translation="MAAYIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVRLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHTVNIDEAQTAVANVARFDVFQLAGA
                     WMKGDVPRVCRLLDGLQEEGEEPVLLLWAVAEDVRTLIRLAAVLKQGQGVQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEEGDAWTVFKRLVVSLAE"
     gene            complement(3252..3731)
                     /locus_tag="WGR04_001465"
     CDS             complement(3252..3731)
                     /locus_tag="WGR04_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0794998.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            complement(3783..4562)
                     /gene="pgeF"
                     /locus_tag="WGR04_001466"
     CDS             complement(3783..4562)
                     /gene="pgeF"
                     /locus_tag="WGR04_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ0794999.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALDGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTTVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            4852..5043
                     /locus_tag="WGR04_001467"
     CDS             4852..5043
                     /locus_tag="WGR04_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795000.1"
                     /translation="MFQQGMAHQAPHCVLPERDYPPIRFYRYKQMGFNRKGMGIKSIS
                     DISRAMPSENQSPLSDGIV"
     gene            5120..6067
                     /locus_tag="WGR04_001468"
     CDS             5120..6067
                     /locus_tag="WGR04_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ0795001.1"
                     /translation="MNILSKISSFIGKTFSLWVALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMAYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(6220..7344)
                     /gene="rluD"
                     /locus_tag="WGR04_001469"
     CDS             complement(6220..7344)
                     /gene="rluD"
                     /locus_tag="WGR04_001469"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ0795002.1"
                     /translation="MQNTSFDNESDYSDDSDFVSASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVQQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAGDPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            7343..8146
                     /locus_tag="WGR04_001470"
     CDS             7343..8146
                     /locus_tag="WGR04_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ0795003.1"
                     /translation="MKKILLTVSLGLALSACATKGTVDKDAQITQDWSVEKLYTEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYKKLDKPLLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(8216..10435)
                     /locus_tag="WGR04_001471"
     CDS             complement(8216..10435)
                     /locus_tag="WGR04_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ0795004.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRVWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPLAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLVSPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNASGIRYLDYLVLSHHDSDHDGGFQAVGKIPN
                     SGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         2592 a         3210 c         2674 g         2288 t
ORIGIN      
        1 gttaatccac tatacatttc ccttccttgc agcaacaaaa gcgcgtgatt ccaatctgcc
       61 gctgcaaaat cttcaatttt tcacacccga aaacgcttgc cgccatacag atgtgatata
      121 ttgattccat tttaacgggc ttcggagaac gacatgagca gcggaaatat ccacatccac
      181 agcatgaccg gatttgccaa cgcggcggca gagtgcggca gcaaacgcat caacctcgac
      241 atccgcgccg tcaaccaccg ttttctagat attcaaatca ggatgccgga tgatttgcgc
      301 catttggaaa gcggtatccg cgagaaaatt tcatcccata tcgcgcgcgg caaagtcgaa
      361 tgcaaaatcc aaattcaaga tgcggaaaac ggcagtcagt ccttggaact gaatcgcgat
      421 ttggtcggac aactggcaga aatcaacaaa gacctccgca agcatcacga tttggcaaaa
      481 ctgggcgttg ccgacatcct gcgttttccc ggcgtattgg caagccaaag ggaaaatacg
      541 gaagaattgg caaaaagcat taccgaactg accgaaaaag cgttaaaaga ctttactgcc
      601 gcacgccggc gcgaagggaa aaaattgggc gagcacctgt tacaacgcct cgaaagcatg
      661 gaagaaatca tagatgcatt gagcgaactc tttcctaccc tgctggaaac acataaagaa
      721 aaaatccgcg cccgcctcgt cgaagcagtc ggcgacatca ataatgacag gctgcaacag
      781 gaatttgccc tctttattca aaaatccgac atcgacgaag aattcagccg cctgcgcacc
      841 cacattgccg aagtgcgccg tatcgttacc gaacacaaag gcagcagcgg caaacggctg
      901 gacttcctga tgcaggaatt aaaccgcgaa gccaacactt tgggcagcaa atccattgcc
      961 gccgaatgca cccaagcctc ggtcgaactg aaagtcttaa tcgagcagat gcgggaacaa
     1021 gtgcagaata tcgaataatc ggtttccccg ataagcaaat gccgtctgaa agccctttca
     1081 gacggcattt tttcaggttg aaacctttgc ggtataaagg cttaaggctt tcctttgacc
     1141 gccaccaatc ttgttcctgc aagaaaatca tacagaaact gccgatcggg attcagcagt
     1201 gcgaaccccc acggtaaaat cagccaaatc aatgccgcgc cggccgcgcc cttgggcgga
     1261 atgccgagga agtggcgtaa tccggcatag gctaacatag ggataaatac gataaatatg
     1321 cacgcccaaa taaagcgcag gcgcagcagg tgcaaagacg gctgtatgcc gttaaggtcg
     1381 cacaagccga ttttccatgt cctcatcgcc aaggtctgcc cctgaccatg ccagttggcg
     1441 cggaaataaa gccaccatgc tcccattatc aggatacttg ttaccaatgc agaaaccgcg
     1501 atagaaacgg gattcagaaa aatggcggca ataccggcaa acaatgctgc caaacaggtt
     1561 gccgcaccga ccagcagcat ttcgtacagc atggcggcaa agcggcgttt gagcggggcg
     1621 gttttcagta tcatcatctt ttcaatcgga atatgccgtc tgaaacgcca acaggcttca
     1681 gacggcattc atgcagatat gtctcatgtg gttaacggtg cggacgtttc tgaccacgcg
     1741 actgttttgc cgccatttga ttgatttcct gacgtgtcgg gcgttttttg aacatagcgt
     1801 gcgcctcgtc caccgaacat ttttgcgaca ggcagatcag ggtaatcgca tctttctggt
     1861 gttgtccgcc gctgtggtac atacggcgca ggtttgcctc gttgattctg cctttttcgg
     1921 gatcgatgtc gaattttttc aggcgttcga taaactggcg ttgctgtcgg cgggatgtcc
     1981 aaacaaaacc gcccgcagcg gcagcgacaa tgatggcggc aagcaggatg gcaagcggcc
     2041 aggaatcgac aatcaatacg ataatgaggc ttaaaacggc ggtggtcagt aaaatcaggc
     2101 cgcccaagcg cagtttggtc ttattgtcca tagggttaat gttcctttat tgaggtggtc
     2161 gtgccgtctg aaatgttttc ggacggattg gtgaatactt ttcaggatta acggtatttt
     2221 acagtatttt cggattttgg ggattaccgc tttattccgc cagcgacacg acaagccgtt
     2281 tgaataccgt ccatgcatcg ccctcttccg cgcctttgat gattcggtcg atttgggcgc
     2341 aggttttgag cgcgtcaagc aggcggacga cggaaatccg cttgaccgca agcggcgcga
     2401 gcgtctgctt gtcgccccaa agtctgagac tgttgcgtac ggattggaca ccctgcccct
     2461 gtttcaggac agcggcaagc cgaatcagcg tccgcacgtc ttcggcaacc gcccacagaa
     2521 gcagtaccgg ttcttcgcct tcctcctgca atccgtccaa caacctgcat acgcgcggga
     2581 catcgccctt catccatgcg ccggcaagct gaaaaacgtc aaagcgggca acattggcaa
     2641 cggcggtttg cgcctcatcg atattgacgg tatgcccttt cggatacagc agcgcgagct
     2701 tgtcgatttc ctgacgcgcc gccaacaaat tgccttccac gcgctcggca aacagtgcca
     2761 atgcgtctgc ctcgataccc aaaccgattt tgtccagccg tccgcgtatc cattggggca
     2821 aggccgccgc gccgaccggt ttggcttccc acacttcccc ctttgccgcc aatgcggcaa
     2881 accatttgga ctggagctgg gttttctcca gtttgggcag caaaaccagc cttaccgtat
     2941 cttccggcag gcgggcggca aaatcctgca acgcctcgcc gccggttttg ccgggttttc
     3001 cgttggggat atgcagttcc aacagcttca aatcggcaaa cagacccgca ctgcctgcgg
     3061 tttgcaacag ttcgttccaa tcgaaggcat tgtctgccgt ataaacttcc cgattaaggt
     3121 aaccttgttt cttcgccgcc gccctcaatg cgtccaatgc ctcgatacgc aacagttctt
     3181 cctcgccgtg gatgacgtac aggggtttca aaggcgcgtc cgtatcaatg cgtccgatat
     3241 atgccgccac gtcattccgc cttcagaaag gtcaggcggc ggacaatctg ttcggcggca
     3301 tcctgccgca tttccgccca caatgccgct tcctcttcct gtttgcccaa gatctcgttg
     3361 tcggcataag caaggacgcg gcggacggac acggtcatcg gtttgccgac cggctcgccg
     3421 cgtttcaata cctgcgcttc aaccgtcaat atcaaaagat attcgttgat gactgccgca
     3481 cgggtaacgg tgtaggtttc cttgttttgg gaaacgctgt ctatacgcag ggtcatctgc
     3541 gcgccggcag catcgtccac cctgcccgaa gcctgataca gcgcggtttc caaaggaaac
     3601 tgcaatgcct gtccgccttc gatgtgccag ctccggtagg tcagcggcgg agaaatgccg
     3661 tctgcacctt tcaggtggaa accgcacgcg cccagcatca gcactgcggc agtaagaaac
     3721 agtttgttca tggcttgcct ttctcaaaag cacaaagtcg gtagattata tcagcggcgt
     3781 gttcagacgg cattgccgtc cagccaaatc aggctcgcca tacgccctgt cgctccgtcg
     3841 cggcggtagg aaaagaaagt gtcccgttcc aaaaccgtac aatgcgtgcc gccatatacg
     3901 ccgcccacgc cttcacgctt cagaatcagg cgggcgagcg cgtaaaggtc ggcaaggaat
     3961 ttgccgccgc ctatgccttc aaatgcggcg gcggcttcgg gcatgggcgt gcagaacgca
     4021 tcaaacacat cctgtccgac ttcaaacgca tccgcactga tggcagggcc gagatacgcc
     4081 atcatttcga cgggcggaac cttcatcgcg gcaatggtgt tttgcaatac gccgcccgcc
     4141 aaaccgcgcc agcccgcgtg tgcggcggca accgtcgtac ccgccctgtc gcaaaacaaa
     4201 acaggcaggc agtctgcggt catcacggca caggcaacct tgcccgtgtc gtccacggaa
     4261 gcgtccgcat cgggcgtgcc gtccaatgct tcggcagcat tgacgacaat ggtgctgtgg
     4321 atttgattga ggtaggcaac gggcaatccg acctgctgtt gcacgatttc gcggttgcgg
     4381 cgcacggctt cgggattgtc gccgacgtgc gtaccgaggt tcaaactctg atacgcacct
     4441 tggctcacgc cgccgttgcg cgtggtaatc agggttttca cattggcggg cgcgggccaa
     4501 tcggcggtca ggaagttttt gcctttcggg gcgagattta gggtttctgt gatggttttc
     4561 attggatatt ggaataggtg tgtggcggaa agggttgatt aactgccttc ggggcaaatg
     4621 aatgcttgtt ttttcgtcat tcccacacag gcgggaattc ggagattcgg gattgttttc
     4681 aaacgttcgg gttcggattc ttgccggtgc aggaatgatg tccttatata ccttctccga
     4741 gctttatata gtggattaaa tttaaaccag tacagcgttg cctcgccttg ccgtactatt
     4801 tgtactgtct acggcttcgt cgccttgtcc tgatttttgt taatccacta tatgtttcaa
     4861 caggggatgg cacatcaagc accgcactgc gtgttgcccg aacgagacta ccctccgatt
     4921 agattctatc gctataaaca gatgggtttc aaccgaaaag gaatggggat aaagtccatt
     4981 tccgacatct ctcgggcgat gccgtctgaa aaccaatctc cactttcaga cggcattgtt
     5041 taggcacggt cattttgcaa attaaggtta aggcgtgtat gcttgctttc ccttaaaaac
     5101 aaacacgcaa cgcattatta tgaatatcct cagtaaaatc agcagcttta tcggaaaaac
     5161 attttccctc tgggtcgcgc tctttgccgc cgccgctttt ttcgcgcccg acaccttcaa
     5221 atgggcgggg ccttatattc cttggctgtt gggcattatt atgttcggta tgggtttgac
     5281 gctcaaacct tccgacttcg atattttgtt caaacatccc aaagccgtca tcatcggcgt
     5341 aatcgcacaa ttcgccatta tgccggcaac cgcctggctg ctgtccaaac tgttgaacct
     5401 gcctgccgaa atcgcggtcg gcgtgatttt ggtcggctgc tgcccgggcg gtacggcttc
     5461 caatgtgatg gcctatctgg cgcgcggcaa tgtggctttg tcggttgccg ttacgtctgt
     5521 ttccaccctg atttccccat tgctgactcc cgccatcttc ctgatgcttg ccggcgaaat
     5581 gctggaaatc caagcggccg gtatgttgat gtccatcgtc aaaatggttt tgctccccat
     5641 tgttttgggt ttgattgtcc ataaggtttt gggcagtaaa accgaaaagc tgaccgatgc
     5701 gctaccgctg gtttccgttg ccgccatcgt gctgattatc ggcgcggttg tcggggcaag
     5761 caaaggcaag attatggaaa gcggcctgct gatttttgcg gttgtcgtac tccacaacgg
     5821 catcggctac ctgctcggct tctttgccgc caaatggacc ggcctgcctt atgatgcaca
     5881 aaaaacgctg gccatcgaag tcggtatgca aaactcgggg ctggccgccg cgctggccgc
     5941 cgcacacttt gccgctgcgc cggtcgttgc cgttccgggc gcattgttca gcgtgtggca
     6001 caatatctcc ggctcgctgc tggcaactta ttgggcggcc aaagccggta aacataaaaa
     6061 accctaaacg cctttcatca taatgccgtc tgaacggggc aaacgaaacc ggcggtttcc
     6121 gcccgacccc ttcagacggc ctttgtttga aatcggcggg aacgattgcc cggctgctcc
     6181 gattcggctg cctgcgttcg ccccgccttt caagccgcct cactccctta cataaaccac
     6241 ttcgacatcg tagtcgtctt cgttccaatc gtcatcgtcg tccgcgccga atttgtcctg
     6301 ccattcctct tcattgctca aagacgaatc caaaccggct tcaagacgga ggacagacaa
     6361 taaatgatat atgtcgtccg gaatcggtgc ctcaaacgaa acggtttcgc cgctttccgg
     6421 atgggtaaaa ctcaagcggt aggcgtgcaa tgcctgacgc gcacccaaac ttttaacggc
     6481 ttctttcacc gtgtcgccgc acggatggcg cgggttgccg taaaccgggt cgccggcaag
     6541 cggatggttg gcctcacgca tatggacgcg gatttggtgc gtcctgcctg tttcgagcga
     6601 gcattcgata tagctgtggg caagatagcg ttccaacact ttgacgtggg taacggctgg
     6661 tttgccgccg aatttgacga ctgccatttt caggcggttg tgcggatcgc gtccgatttg
     6721 ggtttcgatt ttaccgtcaa agggaacgat gccgttggcg acggcgcggt agatgcgttt
     6781 gaccgtgcgc tcttgaagct gttgcacgag ggaattttgc gccggcaggg ttttggcaac
     6841 caccatcagc ccgctggttt ctttgtccaa acggtgtacg atgcctgcac gaggtacttg
     6901 gctcaattcg ggacaatgcg ccaagaggcc gttgagcagc gttcccgtcc agttgccagc
     6961 cgccggatgc accaccagtc cggccggttt gttgacgacg atgacggtat cgtcttcgta
     7021 aacaatatcc aaagccatag gctctggaac aaacgccaga ttttcctcac tcggacggac
     7081 ggttacacaa atttgctcgc cgcctatcat tttgtctttg ggttgcgaag gtttatcgtt
     7141 tacaataacc gcgccttctt taatccatga tgtcaggcgg ctgcgcgagt agtcgggcag
     7201 aagtttcgcc aacaccgcat ccaaccgccc gccggcaagc tcgagcggaa cggtcagacc
     7261 gatacggttt tcagtttcgg aagctgacac aaagtctgaa tcatcgctat aatcggattc
     7321 attatcaaag gaagtattct gcatgaaaaa aattctttta acggtttcat taggtttggc
     7381 actgagtgcc tgtgccacca aaggtacggt agataaagat gcccaaatta cccaagactg
     7441 gagtgtggaa aaactctata ccgaagccca ggacgaattg aacagcagca attatacgcg
     7501 ggctgtcaag ttatacgaaa tcttggaatc gcgcttcccc accagccgcc atgcccggca
     7561 gtcgcaactg gataccgcat acgcctatta taaagacgat gaaaaagaca aagctctggc
     7621 ggcaatcgaa cgcttccgcc gccttcaccc gcaacacccg aatatggatt acgcgctgta
     7681 tctgcgcggc ttggtgctgt tcaacgaaga ccagtccttc ttgaacaaac tggcctcgca
     7741 agactggtcc gaccgcgacc cgaaagccaa ccgcgaagcg taccaggcgt ttgcggaact
     7801 cgtccaacgc ttcccgaaca gcaaatacgc cgccgatgca accgcacgca tggtcaaact
     7861 ggtcgatgca ctgggcggca atgaaatgtc ggtggcgcgc tactacatga aacgcggtgc
     7921 atacatcgcc gccgccaacc gcgcccaaaa aattatcggc agctaccaga atacgcgcta
     7981 tgtcgaagaa tcgctcgcca tattggaact tgcctacaag aagttggaca agcccctgct
     8041 tgccgccgat acgcgccgcg tgttggaaac caacttcccg aaaagcccgt ttttgacgca
     8101 cgcttggcag cccgacgata tgccctggtg gcgttactgg cactaagccc atcctcgaaa
     8161 tgccgtctga agccctttca gacggcattt tccgttccgg caccagcctt tccgttcact
     8221 caaacggttt tttctgccaa tagaatttat accctctcaa acgttgggcc ttcacgccgc
     8281 cgcgccccaa gccgaattgc agcgcgcccg acaaatcggt acgcagcagt ttgatgccgt
     8341 gtgcgcggac acggttctgc accgcttcgg tcggatgttt gtaggcgttg gcataaccgc
     8401 ttgaagcaac ggcatattcg ggcgagacgg cattgaggaa cacgcccgac gaggacgtat
     8461 tgctgccgtg atgtcccaaa accaaaacct ggctgtacag gttgcctcca tacttgccga
     8521 ccaggctttc ctcgcccttc gtatccaaat cgcccgttac cagcagtgcc gcaccgcccg
     8581 ccacaacacg caaaacacaa cttttcccat tatcatcgat gtttttgcgt tcagacggca
     8641 tcaaaaactc gaaatctacg ccgtcccatt gccaacgctg ttccgcacaa tgccgcgccc
     8701 cctcataaaa ttccggctgt ccggcataaa tcccgctgtt cggtatcttg ccgactgcct
     8761 gaaaaccgcc gtcgtggtcg ctgtcgtgat gcgacaaaac caaataatcc aaatacctga
     8821 ttcccgacgc attcaagctc ggcacaatcc ccatctgcgc cgccgctgcc gttccggtgt
     8881 caaacaaaag atgatgattt gccgtctgaa ccgacaccga caaaccctgc cccgcatccc
     8941 aaaccgtaac cgcaacctca ttttccggca cgccgggtga acggtaaaac acaaaccctg
     9001 ccaacagcaa caccgcccac ggacgcaaac ccaagccgcg cggcagcagc aacagcaaag
     9061 cggcacacac cgccaacacc aacagcggca aaggtgcgga ggcaacggca aactcgggcg
     9121 acacatcggc aagccacacc aaaaaccgca aagtatattc cgccaaaaat gcccccaact
     9181 gttgcaaagg cgcaaacggc acgaccgaac ccagcaacgc cagcggcgtc aatacccaag
     9241 aaaaccacgg aatcgccacc gcattgacca aaggactgac caaaggcagc gaagcaaaca
     9301 gataaccgag caaaaccaat gacaacaccg aagccgccca ctgcccgcgc aaagcagttt
     9361 gccgtttccc ctcgtgcaaa cgccccgaac acgcccatat cagggccgcc accaaaccaa
     9421 aagacagcca agttcccaca cccaagaccg ccaaagggtc gaacagcagc accgccgcca
     9481 acgcctgcca ccacgttgcc cacgccgaca atcttcccct gcgccaagcc cacgcaaacg
     9541 ccgccaacat caaaacactg cgctgcgtcg gcacggaaaa accggcaagc agcgcgtaaa
     9601 acagcgcgcc tgcacacccc gccgccaaaa cccacacgcg cggccgggca ggaaggcgcg
     9661 gggaacaggc aagcagccgc ttcgccagcc acgcaaacat caccgccacc atcgtaacgt
     9721 gcaaacccga aatactgacc aaatgcgtca gccccaacgg tcgaaacgcc tgccacaatt
     9781 cggggcgcaa tgccgactgt tcgcccacgc tcaacgcgcg catcagcccg atgccgtctg
     9841 aaagcccgcc gtccgcatcc gcctgctgcc aattacggct gatgcggctg cgccaaaccg
     9901 caatccccca accgcttccg ccatgcagca aaaccctgtc cgcaccgacc gtccccgcgc
     9961 cgcctattcc gttggataat gcccacgcct cacggttcaa accgcgcagg ttcaactccc
    10021 cgacgacagg gtgcacacgt gccgttatcc gccatctgct cccgaccgcc cattcgcgcc
    10081 gtttgtagtc cgacagcagc aaatcaaacg tccgaccacc gctgtccaca gcctttgccg
    10141 caaactgcac acgccgcccg tccgacctcg gcatatccgc cacttccacc gtcaaaggca
    10201 cgcctgaaac ggcttctgcc cgccattgcg aagacagtgc cgcttccgtt ctgaatacgc
    10261 cgtaagccgc gcccgccaac acgcacagca tcagaccggc aaacgcaaaa cgccttgcaa
    10321 acacagcaaa aaccgcaaaa gccgccagcc aaaacagcca atgcggcacg accggcagcg
    10381 caaacgaagc cgccgcgccg acaacccaac acggcaaaaa ccaacgacgg aacacgtctc
    10441 cccaccccag atatttcaaa aataaacgga taataaagca aatccgaaaa accgtcataa
    10501 caagatttga aaatgatata gtggtttaaa tttaaatcag gacaaggcga cgaagccgca
    10561 gacagtacaa gcagtacgac aaggcgaggc aacgctgtac tggtttttgt taaaccacta
    10621 taagaatgca aacgaaaagc ctcagtatta tccgagacaa ccgcaaactt tccatcgtca
    10681 ttcccacgaa cccacatccc gttattacca cgaaagcggg aatctaggac tcagggttaa
    10741 gaaaacctac atcccgtcat tccc
//
LOCUS       DAWFJR010000063        10608 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWFJR010000063 DAWFJR010000000
VERSION     DAWFJR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10608
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.063"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..124)
                     /locus_tag="WGR04_001472"
     CDS             complement(<1..124)
                     /locus_tag="WGR04_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0795005.1"
                     /translation="MMKIKALMIAAALLAAADVHAAPQKAKTASAKAAKAAKAAK"
     gene            complement(226..2634)
                     /locus_tag="WGR04_001473"
     CDS             complement(226..2634)
                     /locus_tag="WGR04_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ0795006.1"
                     /translation="MARLFSLKPLVLALGLCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVQGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GMTFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKNDAVMLDGSVGKKPRSRS
                     DWVAFASSGIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            2704..3708
                     /locus_tag="WGR04_001474"
     CDS             2704..3708
                     /locus_tag="WGR04_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ0795007.1"
                     /translation="MQRQIKLKNWLQTVYPERDFDLTFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDFGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(3765..4463)
                     /locus_tag="WGR04_001475"
     CDS             complement(3765..4463)
                     /locus_tag="WGR04_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0795008.1"
                     /translation="MKSKHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAH"
     gene            4490..4681
                     /locus_tag="WGR04_001476"
     CDS             4490..4681
                     /locus_tag="WGR04_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795009.1"
                     /translation="MLEINRNPAILLHFRVLIQFAAGKGSFRIGKPLQTASNPMPSEA
                     VSVVQYAPLPQTGTTVEEQ"
     gene            4681..6219
                     /gene="murJ"
                     /locus_tag="WGR04_001477"
     CDS             4681..6219
                     /gene="murJ"
                     /locus_tag="WGR04_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ0795010.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEAAEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFGIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPS
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLYAAQIWLPFDWAHAGGMQKAARLFILIAVGGGLYFASLAALGFRPRHFK
                     RVEN"
     gene            6351..7307
                     /locus_tag="WGR04_001478"
     CDS             6351..7307
                     /locus_tag="WGR04_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HEZ0795011.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPTYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSRSHYAVELMLAVRSRGWHGVVVHFRSCGGVA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGERGENALPQAAA
                     AVSAPVDAEAAGSRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPTPDEVSEAV
                     TLFQPAHGGHVGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            7356..8138
                     /gene="trpC"
                     /locus_tag="WGR04_001479"
     CDS             7356..8138
                     /gene="trpC"
                     /locus_tag="WGR04_001479"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ0795012.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPPEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHDETELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRADDIEAEVGKLF"
     gene            8204..10504
                     /gene="recQ"
                     /locus_tag="WGR04_001480"
     CDS             8204..10504
                     /gene="recQ"
                     /locus_tag="WGR04_001480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ0795013.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGKQEAIVNALAVGESLMVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADGAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQRLGI
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLDFIRKEMTGQSGIVYCLSRKKVEDVARFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLAVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QNLNGLHDIYGLGEAKTERFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLADTPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDETAKHKRRLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
BASE COUNT         2174 a         2997 c         3057 g         2380 t
ORIGIN      
        1 ctttggcagc tttggcagct ttggcagctt tggcggatgc ggtttttgcc ttttgcggtg
       61 cggcgtggac atcggctgct gccagcaatg cggcggcaat catcagggct ttgattttca
      121 tcattctttc cttatcgttt gaatcctgcc tttgatggcg ggtttggggt tgtcggtgga
      181 atgccgtctt gtgcgggaac ggtgctcctg ccgggtttcc gacggtcagg gccgtttgtt
      241 gcgtccggcg gaaagagagt gggcggggat atagccggga acggcgacat ccatcctgtc
      301 tgcggggttt ctgccgacac tgctgaggtc tttcaactga agtgagaaaa agacagcgtt
      361 tttgtaggtg ttttcgccgg taacgtagcg ttgggcgtac acgcccgcgc cccagcagcc
      421 gcaactgctt ttgtattccg cacccgccag cacctctatc ggttttttgg cttcaaaacc
      481 gtagttgtaa cggacgacgg ccgacaggtt gcgcgtcagc ggccattgtg cggacaggtc
      541 gagctggctg agtttgtcgt aaaaatagga accgtcggac ttcaggtaga ttttttcgtt
      601 gcgcccgtat ttgtagcggg cgttcagcac tttgccctgt gcgggacggt agcttgcacc
      661 gacggcgtag ttctcggcgc gtttgtcgtt ttggttgtag tggatgctgc tgtcgaggat
      721 gaagcggctg ccgatgccgc tggaggcgaa tgccacccag tcggaacggc tgcgcggttt
      781 tttgccgaca ctgccgtcaa gcatgactgc gtcgtttttg aagtagaatt tctgcccgat
      841 gccggcgcgg aaacgctctt cccccgtcgc gccgtccaaa atacggcttt gcacggcggc
      901 ggaaaggctg tttgcggtgt taatcctgtc gttgccgtaa tagaggtttt cacgaaaaag
      961 ctgcccgtag ccgaagctgc tttccgacga atcgaaattg ggcaggtcgt tttgggattt
     1021 ggcaggaata tagttgtaga acaggcgcgg ctcgagggtt tgcaggactc cgccgccgaa
     1081 catccgcgta ttgcgttcga aggtcatgcc gctgtcgatg ttgacgatgg gcagagtgcg
     1141 gctgacgcgt cgggcttctt ggctgccgaa gcggttgagg ctgtaatagg tggcgtgcag
     1201 tccgagtttg ggacggacgt aaccccagct gttgctgaaa tcccatttga tgtcgggata
     1261 gacgacgagg cggctgccgt cttggcggct gtcgtggctg aagcgggtaa attgggcgga
     1321 cacgccgatt tgcgccctgc cggtgttttt gcgccaatcg gcggaaaggc gcggcatcag
     1381 ggcatacggt ttgtctttgt agccgctttg gtttgccagc gtctggtatt tcagaaccga
     1441 aaggccggca ttcaggctgc cgcccgccgc cctgccgcca taatccagcc atacacggcg
     1501 gttgaggttg acgttgccgg cgatttcttt gttgccgtaa aagtcgcggt agtagccgct
     1561 gtcggagact tggttgaaat cgacacccgc ctgaagcgtg tcggaaatgt cgtgccggtg
     1621 ctgccatttc gcctgatagc ggttattcct gccgcttttc ttgtcgtgcg gcagccaggt
     1681 caggtcggac tggccggcat aatccggccg caggtagcgt acctgcccgt caaagaccgc
     1741 gccgcgttcg ccgatcacgc cgggcgcgaa cgtggcatcg agattggggg caaggttgaa
     1801 ataataggga acggaaaggg aaacgccgtc cgaaccggcg gacagtgagg gaacgagcag
     1861 gccgcttttg cggttgccgt caagcgggaa gtccgcccaa ggggtgtaga aaatgggaac
     1921 gccgccgaac acgaaggcgg cgtgtttggc aacgcctatg cctttttccc gatcggcttc
     1981 gacggaagcg gccttgacat accagccggc atcgccggcg gaacaggtgt tgaattgggt
     2041 ttccgtcagt ttgtaatgcc cttcgcccaa catttcggcg gtgcggctga cgctttgcag
     2101 ccgccgtccg ccgtgttcgg tttccatacg gacgttgtgc gcttccccgg tctgctgctc
     2161 gagattgtag gtcagggttt cgccccgaat cagcgtaccg tcctgttgga gggcgaaccg
     2221 gtcgcctgcg gtaacggtgt cgcccgactg gtcgtaatcc gcccaatcgg tattgagggt
     2281 cgtccggttg cgttcgacga cgacgttgcc ttcggcacgc acctgcacct gcgactgtcc
     2341 ttccaccttg tcggcaacaa tgcgcgtata gtcttcgggg atggatgctt cgccgctgcc
     2401 ttggacggcg gcttcggttc tctcggggct gccgctttcg ttactgcaaa acaggcaggt
     2461 cgaaccgagg ctcaggctgg taggctgtat gggttcggac acgctccgaa cgcttcctcc
     2521 ggcggtcgga ttgtccgttt cctccgccgc aacggcatcg gcggcggcgc aatgcgtgcc
     2581 gaagcagagg cccaatgcca gcaccagtgg tttgagtgaa aataaacgag ccaaaatcgc
     2641 ccctcaagtc ggtttgccag ttagaatagc gtttattgta acctgaaatg ctttagtact
     2701 gttatgcaac gacaaatcaa actgaaaaat tggcttcaga ccgtttatcc cgaacgggac
     2761 ttcgatctga cttttgcggc ggcggatgct gatttccgcc gctatttccg tgcaacgttt
     2821 tcagacggca gcagtgtcgt ctgcatggat gcaccgcccg acaagatgag tgtcgcacct
     2881 tatttgaaag tgcagaaact gtttgacatg gtcaatgtgc cgcaggtatt gcacgcggac
     2941 acggatctgg ggtttgtggt attgaacgac tttggcaata cgacgttttt gaccgcaatg
     3001 cttcaggaac agggcgaaac ggcgcacaaa gccctgcttt tggaggcaat cggcgagttg
     3061 gtcgaattgc agaaggcgag ccgtgaaggg gttttgcccg aatatgaccg tgaaacgatg
     3121 ttgcgcgaaa tcaacctgtt cccggaatgg tttgtcgcaa aagaattggg gcgcgaatta
     3181 acattcaaac aacgccaact ttggcagcaa accgtcgata cgctgctgcc gcccctgttg
     3241 gcgcagccca aagtctatgt gcaccgcgac tttatcgtcc gcaacctgat gctgacgcgc
     3301 ggcaggccgg gcgttttaga cttccaagac gcgctttacg gcccgatttc ctacgatttg
     3361 gtgtcgctgt tgcgcgatgc ctttatcgaa tgggaagaag aatttgtctt ggacttggtt
     3421 atccgctact gggaaaaggc gcgggctgcc ggcttgcccg tccccgaagc gtttgacgag
     3481 ttttaccgct ggttcgaatg gatgggcgtg cagcggcact tgaaggttgc aggcatcttc
     3541 gcacgcctgt actaccgcga cggcaaagac aaataccgtc cggaaatccc gcgtttctta
     3601 aactatctgc gccgcgtatc gcgccgttat gccgaactcg ccccgctcta cgcgctcttg
     3661 gtcgaactgg tcggcgatga agaactggaa acgggcttta cgttttaaac ccaatcaaaa
     3721 tgccgtctga aaaccaagtt tcagacggca ttttccaaac gggcttagtg cgcggctttt
     3781 tgttcttcgc gtactttgtc cgccaaaagg tcgatggtgt tcataccgga ctcccagtcg
     3841 gcaaattcga ctttatattt gccgccgacg ataaccgtgg gcgtaccgtc gatttggaag
     3901 gtttcggtca gctcctgcat tttgtcggcg cgcgcctggc tttcgggaga ttcgtaagcg
     3961 gcaaggactt ttttgccgtc aaaggcggtt tgttcgccca gccatttttt gaggacttcc
     4021 ggctcttgca gcttgatttt ttggttgacc atcgcatcga aaatatggct gttcgccaca
     4081 tctttgctgt cggcggcagc catatcgacg gcggcggcga ggcgtgccag cgtcagcatt
     4141 tctttctgcc agacgacgtg ttcggtacgc aggtacatat cgtctttaaa agacttggcg
     4201 tgtttgctta aaacaggttc gaggtgggcg caatgcggac agaaatagcc gaaaaactca
     4261 aggacttcaa ccttgcctgc ctgctgttgg ggaatcgggt tggcaaggac ggtatagttt
     4321 tgcccttcga ccagccctgc cggggcggcg gctgccgaag cggcaggtgc gctgtcggcg
     4381 gggacgctgg tttggacttt gctgtcgcac gcggcaaggg cgaacagggc ggcaacgccg
     4441 agggcgaggt gtttggattt catacggctc tccgtgatgt tggaaacaga tgttggaaat
     4501 caatcggaat ccggctattt tattgcattt ccgcgtgttg atacagtttg ccgccggaaa
     4561 aggaagcttt cgtatcggga aaccgcttca gacggcatca aacccgatgc cgtctgaagc
     4621 ggtttctgtc gtacaatacg cgccgttgcc ccaaacgggt acgactgttg aggaacaatg
     4681 atgaatatgc ttggagcttt ggcaaaagtc ggcagcctga cgatggtgtc gcgcgttttg
     4741 ggatttgtgc gcgatacggt cattgcgcgc gcattcggcg cgggcatggc gacggatgcg
     4801 ttttttgtcg cgttcaaact gcccaacctg cttcgccgcg tgtttgcgga gggggcgttt
     4861 gcccaagcgt ttgtgccgat tttggcggaa tataaggaaa cgcgttctaa agaggcggcg
     4921 gaggctttta tccgccatgt ggcggggatg ctgtcgtttg tactggtcat cgttaccgcg
     4981 ctgggcatac ttgccgcgcc ttgggtgatt tatgtttccg cacccggttt tgccaaagac
     5041 gcggacaagt tccaactttc catcgatttg ctgcggatta cgtttcctta tatcctattg
     5101 atttctttgt cttcatttgt cggctcggta ctcaattcct atcataagtt cggcattccg
     5161 gcgtttacgc ccacattcct gaatgtgtcg tttatcgtat tcgcgctgtt tttcgtgccg
     5221 tatttcgatc cgcccgttac cgctctggcg tgggcggtct ttgtcggcgg cattttgcaa
     5281 ctcggcttcc aactgccctg gctggcgaaa ctgggctttt tgaaactgcc caaactgaat
     5341 ttcaaagatg cggcggtcaa ccgcgtgatg aaacagatgg cgccggcgat tttgggcgtg
     5401 agcgtggcgc agatttcttt ggtgattaac acgattttcg cgtcttatct gcaatcgggc
     5461 agcgtttcat ggatgtatta cgccgaccgc atgatggagc tgcccagcgg cgtgctgggg
     5521 gcggcactcg gtacgatttt gctgccgact ttgtccaaac actcggcaaa ccaagatacg
     5581 gaacagtttt ccgccctgct cgactggggt ttgcgcctgt gcatgctgct gacgctgccg
     5641 gcggcggtcg gactggcggt gttgtcgttc ccgctggtgg caaccttgtt tatgtaccga
     5701 gaattcacgc tgtttgacgc gcagatgacg cagcacgcgc tgattgccta ttctttcggc
     5761 ttaatcggtt tgatcatgat taaagtgttg gcacccggct tctatgcgcg gcaaaacatc
     5821 aaaacgcccg tcaaaatcgc catctttacg ctcatctgca cgcagttgat gaaccttgcc
     5881 tttatcggcc cactgaaaca cgtcggactt tcgcttgcca tcggtctggg cgcgtgtatc
     5941 aatgccggat tgttgtttta cctgttgcgc agacacggta tttaccaacc tggcaagggt
     6001 tgggcagcgt tcttagcaaa aatgctgctc tcgctcgccg tgatgggagg cggcctgtat
     6061 gccgcccaaa tctggctgcc gttcgactgg gcacacgccg gtggaatgca aaaggccgcc
     6121 cggctcttca tcctaattgc cgtcggcggc ggactgtatt tcgcatcact ggcggctttg
     6181 ggcttccgtc cgcgccattt caaacgcgtg gaaaactgac cgatgccgat atcctttttc
     6241 ggcaaacgct tttccacgcc tatattcggg cgcgcatccc ccacgtccgc accagccgga
     6301 cgggctgccg catcatcaat gccgtctgaa aacagaaaaa ccgatacctt atgattttga
     6361 caccgccgga cacaccgttt ttcctccgca acggcaatgc cgacacgatt gccgccaaat
     6421 tcctgcaacg ccccgcaccc acataccgcc gcgagctgct tcccgacagc acgggtaaaa
     6481 ccaaagtcgc ctacgacttt tcagacggca tttcgcccga tgcgccgctg gtcgtgctgt
     6541 ttcacggttt ggaaggaagc agccgcagcc attacgcggt cgaactgatg ctcgcggtac
     6601 gcagccgcgg ttggcacggc gtagtcgtcc atttccgcag ctgcggcggc gtagcgaaca
     6661 ccgccccggt gttctaccac ttgggcgata ccgccgaaat cgcctttact ttagacacgc
     6721 ttgccgcgcg ttaccgtgaa atatacgccg tcggcgtatc gctgggcggc aacgcgctgg
     6781 caaaatattt gggcgaacgg ggcgaaaacg cgctgccgca agccgctgcc gccgtatccg
     6841 cccccgtcga tgcagaggcg gcaggcagcc gcttcgacag cggcatcacg cggctgctct
     6901 acacgcgcta cttcctccgc accctgatac ccaaagcacg ttcgctccaa ggctttcaga
     6961 cggcatttgc cgcagggtgc aaaacactgg gtgagtttga cgaccgtttc accgcaccgc
     7021 tgcacggctt tgccgaccgg cacgactact accgccaaac ttcctgcaaa ccgctgctca
     7081 aacacgttgc caaaccgctg ctcctgctca atgccgccaa cgaccccttc ctgccgcccg
     7141 aagcgctgcc gacaccggac gaagtgtccg aagccgttac cctgttccag ccggcacacg
     7201 gtggtcatgt cggctttgtc agcagcaccg gcggcaggct gcacctgcaa tggttgccgc
     7261 agaccgtcct gtcctatttc gacagcttcc gtacaaacag gcgttaacgg tttgatgcta
     7321 atattcccct ttcccagacg aatacggaac ccgacatgac cgacatcctc aataaaatcc
     7381 ttgccaccaa agcacaggaa gttgccgccc aaaaagccgc cgtcaacgcc gaacacatcc
     7441 gcgcacttgc cgcagaagcc gcgcccgtcc gcagcttcat cgattcgata cgcggcaaac
     7501 accgcctaaa cctgcccgcc gtcattgccg aaatcaaaaa ggcaagcccg agcaaaggcc
     7561 tgatccgtcc ggacttccga ccgcccgaga ttgcccgcgc ctatgaaaac gccggggcgg
     7621 cgtgtttgtc cgtactgacc gacgaaccct atttccaagg ttcgcccgaa tacctcaaac
     7681 aggcgcgcga agccgtatcg ctgcccgtgc tgcgcaaaga cttcatcatc gacgaatatc
     7741 aggtttatca ggcgcgcgca tggggggcgg atgccgtcct gctgattgcc gcagcactgg
     7801 aacaggaaca attggaacgc tttgaagcgg tggcgcacga attgggcatg accgtcctgc
     7861 tcgagctgca cgacgaaacc gaattggaaa aatgccgcaa cctgaccacg ccgttgtggg
     7921 gcgtaaacaa ccgcaacctg aggacttttg aagtctccct cgaccaaacc ctgtcgctgc
     7981 tgcccgcact ggaaggcaaa accgtcgtta ccgaaagcgg cattacgggc aaggcggatg
     8041 tggaatttat gcaatcgcgc ggcgtgcaca ccttcctgat tggcgaaacg ttcatgcgtg
     8101 ccgacgatat tgaggcggaa gtgggcaaac tcttctaaat ctcgatttca gacggcatat
     8161 tgccgccgac caatcgacac ccattaccgg acacggaacc atcatgcacc gacccaccgc
     8221 caaacagatt ctgcacgaag tattcggtta tcccgaattt cgcggcaagc aggaggctat
     8281 cgtcaatgct ttggcagtcg gcgagagttt gatggtgctg atgccgacgg gcgggggcaa
     8341 gtctttgtgt taccagattc cggcgctgat gcgcgaaggc gtggcggttg tcgtatcgcc
     8401 gctgattgcg ctgatgaacg accaagtggc cagcctgcat gtggccggca tcgaagcggc
     8461 ggcagtcaac agcggcacat cggcagatgg ggcgcgcgag attgccgaca agcttgccca
     8521 aggccgtctg aaactgcttt atgtcgcacc ggaacgccta gttaccgacc gctttttgcg
     8581 ttttctcgac caacaaaccg tcagcctgtt cgccattgac gaggcgcact gcgtcagcca
     8641 gtggggacac gatttccgcc ccgaatatca acggctcggt atacttgccg aacgctatcc
     8701 gaacgttccg cgcatcgccc tgaccgccac cgccgatgcc gccacgcgcg ccgacatcaa
     8761 gcattatctg cacttggacg atgcgcccga atttgtctcc agctttgacc gcccgaatat
     8821 ttattatcag gttatcgaaa aaaacaacgg caaaaaacaa ttgctggatt tcatccgcaa
     8881 agaaatgacg gggcaaagcg gcattgtgta ttgcctaagc cgcaaaaagg ttgaagatgt
     8941 ggcgcggttt ttgcgtgaaa acggattaaa cgcgattccg tatcatgccg gtctgagtat
     9001 ggacgtgcgc gaggaaaacc aacgccgctt tacgcatgaa gacaatatta tcgtggtggc
     9061 gaccgtggcg ttcggcatgg gcatagacaa acccgacgtg cgctttgtcg cccatctcga
     9121 tatgccccag agtgtcgaac atttctatca ggagtcgggg cgcgccggtc gggacgggct
     9181 gcctgccgcg agctggctgt gttacggttt gaacgattgg gtgttgctgc gcgaacggat
     9241 tgccgaaggc aacagcgacg aggtgcaaaa gcaaatcgaa atgcaaaaac tcgatgccat
     9301 gcttgccgtc tgcgaaaccg ccgcctgccg ccgcgtactg ctgctcaaac atttcggcga
     9361 agcatccgaa ccctgcggcc attgcgacaa ctgcctgcat ccgcccgtac ggtttgacgg
     9421 cacggtgttg gtgcaaaaat tactcagctg cgtgtaccgc gccggacaac gttttgccgc
     9481 cggttacatc accaaccttt tgcgaggtaa aagcgacgat tggatacgcg gcaaccggca
     9541 cgaacaactg tccacattcg gcatcggtac ggagttgtcc gacaaagaat ggcgcagcgt
     9601 catccgccag tgtatcagcc tcggctacct caccgtcaac attacccgat atcaggcatt
     9661 gcaactgacc gaagccgcca aaaaagtcct caaaggcgaa accgaagtga tgctgcgtcc
     9721 gctcaagcgc gacaagcccg ccgcccgcac cctcaaagac aactggctgc gtaccgaacg
     9781 cgaagaacgc ctgtggcagg cattgcgcgt ttggcgtatg aaacaggcag aagccgaagg
     9841 catccccgcc tatatgattt tcggcgacaa aaccctgcgc gaccttgtcg aaaaaatgcc
     9901 gcaaaacctc aacgggctgc acgacatcta cggcttgggc gaagccaaaa ccgaacgttt
     9961 cggacacggc atactcgaag tctgccgcaa cgccgccggc ttcagccgcg acgcggtcat
    10021 ccgtccgcaa accgaacgcg aacaacaact gcgtcaaaaa ctcgaggcct ggcggtatga
    10081 acaggcaagg gcggaaaact gcgccctgca tgccgtcctc tccgacgaaa gccttgccga
    10141 tatgcttgcc gatacgcccg aaaccgaaac cgacctcgaa ggcgtacacg gcttgggcag
    10201 cgtacgcgcc gccaaatacg gacgggacat cctcgccgtc tgccgtccgt tttcagacgg
    10261 catcgatgaa accgccaaac acaaacgccg cctgatgcgc gccctgatcc aatggtgcaa
    10321 cgaaacggca aaacacgaac agtccgaacc ctaccgcatt ctcagcaaag ccgccctacg
    10381 cgccattgcc gccaaacagc cggaaggttt ggcggagctt gccgccgtat acggcgtagg
    10441 cgaagaaaaa gccgcacgtt acggtgcggc ggtgttggcg gtattggaac gaaatgccgt
    10501 ctgaagcccg ttaccaagtt tcagacggca ttgcctctat ttaaaaattc ctgtttttat
    10561 agtggattaa caaaaatcag gacaaggcgg cgagccgcag acagtacg
//
LOCUS       DAWFJR010000064        10543 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWFJR010000064 DAWFJR010000000
VERSION     DAWFJR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10543
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.064"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(63..1205)
                     /locus_tag="WGR04_001481"
     CDS             complement(63..1205)
                     /locus_tag="WGR04_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ0795014.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQAFDGGVSCRGGL
                     EQVYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLNERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIATGRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQTRPVRAPIAGSDSDRRIVQTALDNARSA
                     EVDDIVSFGVADAQSVRPNGENGIMVSNPPYGVRLEEVHALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1254..1676)
                     /locus_tag="WGR04_001482"
     CDS             complement(1254..1676)
                     /locus_tag="WGR04_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795015.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1669..2478)
                     /locus_tag="WGR04_001483"
     CDS             complement(1669..2478)
                     /locus_tag="WGR04_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ0795016.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWTGKPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMVVQTREQWRRAQEKGADAVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2560..2937)
                     /locus_tag="WGR04_001484"
     CDS             complement(2560..2937)
                     /locus_tag="WGR04_001484"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ0795017.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3137..3904)
                     /locus_tag="WGR04_001485"
     CDS             complement(3137..3904)
                     /locus_tag="WGR04_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0795018.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFNMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSIFGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3914..4453)
                     /locus_tag="WGR04_001486"
                     /pseudo
     CDS             complement(3914..4453)
                     /locus_tag="WGR04_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
     gene            complement(4845..4952)
                     /locus_tag="WGR04_001487"
                     /pseudo
     CDS             complement(4845..4952)
                     /locus_tag="WGR04_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4998..5726)
                     /gene="pdxJ"
                     /locus_tag="WGR04_001488"
     CDS             complement(4998..5726)
                     /gene="pdxJ"
                     /locus_tag="WGR04_001488"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ0795019.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(5750..6541)
                     /gene="recO"
                     /locus_tag="WGR04_001489"
     CDS             complement(5750..6541)
                     /gene="recO"
                     /locus_tag="WGR04_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ0795020.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWIGGWPQPQGRALFSGLYVNELMLK
                     LTVREDPLPELYDALAEVMEAVCCKAAYIDDLRRFEWRLLNLLGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(6575..7702)
                     /gene="pheA"
                     /locus_tag="WGR04_001490"
     CDS             complement(6575..7702)
                     /gene="pheA"
                     /locus_tag="WGR04_001490"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ0795021.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(7741..8973)
                     /locus_tag="WGR04_001491"
     CDS             complement(7741..8973)
                     /locus_tag="WGR04_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ0795022.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLG
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLIVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQHFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRITAWRL
                     KTGAHPQSILRWGIVVQFAANLSQFAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMAASTSCGIAL
                     LWLCSHRAWKENEQSEYL"
     gene            complement(9492..10346)
                     /locus_tag="WGR04_001492"
     CDS             complement(9492..10346)
                     /locus_tag="WGR04_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0795023.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT         2530 a         2866 c         2717 g         2430 t
ORIGIN      
        1 tcggccggat attaaaaaaa gccgtctgaa catttttcag acggcatttt tgtacacttt
       61 cctcaacggt tcgatccctg caccatatca atcaggaaca ggcggcaatc gaggttgccg
      121 ttataaagcg gaattttgcg ttttggcgac aggcgcatga atttgggcat ttccctgtcg
      181 ccggtaaaca tgccgacccg ccagccggca tagtgttttt tcaaccacgt acccaactgc
      241 ggataaagcg cctgcaaagc gtggacttcc tcaaggcgca cgccgtaggg cggattggac
      301 accataatgc cgttttcgcc gttcggtcgg acggactgcg cgtcggcaac gccgaaggaa
      361 acgatgtcgt ccacttcggc actgcgtgcg ttgtccaatg ccgtctgaac gatgcggcgg
      421 tcgctgtcgc tgcctgcaat cggggcgcgg acggggcggg tttgcgcttc ggcgcggcgg
      481 cgcaaatccg cccacagcgt tttatcgaaa ttttgcagtt tttcaaaacc gaagcggcgc
      541 atcatgcccg gtgcgcggcc tgtggcaatc caagcggctt cgatagcaat cgtgccgctg
      601 ccgcaaaacg ggtcttgaaa cggctgcgtg ccgtcgtagc ctgccgagag cagcagtccg
      661 gcggcaaggt tttcgcgcag cggggcttcg ccggtatcca ggcggtagcc gcgtttgaac
      721 agggcttcgc ccgaagtgtc gataaagatt tcgacattgc gttcgttcaa aaaggcgtgg
      781 atgcggacat cgggcgcggc tttgtccacg ctcggacgtg cgtcgtaaat gtcgcggaag
      841 gcatcgcaga cggcatcttt gacggtcagt ccgacaaatt ggatgctctt aacgttggca
      901 cgctttgcct cgactttgac tttgaacgtc tgctgcaagg taaaccagtt gaaccagttg
      961 atgtttttgg caagtttgta aatgtcgcgc tcggttcggt atgtgccttt ggtcaggcgt
     1021 agcaggatgc ggccggcggt acgcgagtgc aggttggcgg cgtaaacctg ttccaatccg
     1081 ccccggcagg aaacgccgcc gtcaaacgct tgaacatcgg tacagccgag gctttcgagt
     1141 tcttgcgata aaacggtttc caagccgcgc gggcaggtgg cgaaaagtgt atataaagta
     1201 ttcatagcgt gtcctttccg gacggatgtt ccggcaatgg cggattgaaa ggtttattcg
     1261 gcggtaaatg ccgtctgaaa tgtccggctg ccgattgtaa tgtccgccgt aaaatcgcgc
     1321 ctgccttcga cacagatggg caggcggatg cgtactgcct gccaagtccc cgacggttgc
     1381 cgctcgaaca tatagcggtt gaaacccata tccatatttt tcatactgaa gctgatgctg
     1441 acctgttccg tgccggcggg cgcgtgctcg atataaatat caaacggttt tttggttgaa
     1501 acggcggcgg cgcggacgcg gcttccgtcc ggcagcgtgc aaccctcggt caaatcgcat
     1561 tgcgccgcca cagcttgcgg ctgctgcgcc tgccaccatt gcaacaaaac gagcttgacg
     1621 gcggcaaagg caatcagcag cagtgcggca agcagtaatt tacggttttt attcatgatt
     1681 cttccctatt gtttcatccc ttaccaccac gtgcgccccc aatccgagcc atagttttcc
     1741 ataacgtgca accgtctgtc cgtttgctgc aagtacgagc ggcacggata cgccctgccg
     1801 cagggcttct gccgcctctt gtgcctgaac atcatcgcac acgcgccaaa cgaccgcatc
     1861 cgcgcccttt tcctgcgccc gccgccattg ttcgcgggtc tgcaccacca tccacacgct
     1921 gtcggcgtgt tcgggtctgt cctgccattg cgccgcctcc atcaaaacgt tcgcaccgct
     1981 tccctctgcc gaacccaaag gcaggacgcg gtacgcgccc atactgtttt ctccgtgcaa
     2041 acccgttttc aggctgccgt acaaacggcg cgggacggac agcgaacgca aaagcgcgcc
     2101 gttggcgggc agcatggggg caacggtaaa atcacccgcc ttctgccaag accattcctg
     2161 cccttcgcgg gattgcggtt tgcccgtcca ttggtcgggg ttgacccata ggaatttcag
     2221 gcagacgcgg gcgtgttcgt aggaatggat tttggtcagc caaggcgtgg cggcgaggat
     2281 gcggatgccg agttcttctt caaactcgcg ttgcaaggct tggaagtcgg tttcgcccgc
     2341 ttcgaccttg ccgccggcaa attcccaata tccggcatag ggtttgcctt cggggcgcga
     2401 gctgagcagg tagttgccgt ctgaatcgag caggatgccg gcaacgacgc ggataagggg
     2461 tcgggtgtct tgaatcatgg cgggtacgcg gttggtcggt cggagtgcaa atgagggacg
     2521 ggcaatgccg tcaacgggtt tgccgcattt cagacggcat tatttttcgg caacgacaaa
     2581 cgccaatacg gtgtcttctt cgtcgctcat gctgaggctg acgcggctga tgccttgttc
     2641 ctccagccat ttggacaggg cggggccgta gaaaaattcg ggcttgccca atgcgtcatg
     2701 cccgatgccg atgttgcgga aggaaaccgc gccgcgtatg cccgtgccga cggctttggc
     2761 aaaggcttct ttggcggcaa agcgtttggc gaggtagttg acgggtttgc ccgcttgcgg
     2821 aaattcaagc agctcttccg gagtgaggat gcgcccggca aacgcctgtc cgaatttttt
     2881 gtttaagcgg atgatgcgct tgagggaaac aatgtctgtg ccgatgccgt aaatcatatt
     2941 tgcgctcctt cgcccttggt tgcgggtagt gatgaatgtg atgatgaatg ccgtcaattt
     3001 cttggggcgt taaaatggcg tgaatatcca cttttggggt tttgtcggta acaattttca
     3061 ctgtaacctt ttgcatttga aattctaaaa catcgtgcaa attatttaat aaggagttaa
     3121 ggcatttaat aatgaattaa agtttgatta aacccgaatg cccgattttc agagccaata
     3181 ccaataaaac aataaaaatt aacattttaa aaaaatttgg gctaatctta gtccttaacc
     3241 gaattccaac atacaatcca ataacagaca atacggacag taaaaatatt aaaccgtatt
     3301 cactcttatt taataaccaa tactggtctc ttagcatata tatttgaaca attttcgcca
     3361 aaagatagca tagattgctt gattttacga tacgattttt attttctgtt tcgctaagca
     3421 aaaatattaa caatatggga gacatggcat tggttgaacc gccgatgatg cctgccaaaa
     3481 acccaaaaag aaccatattc ttattattgg caactacttg aatatttttt gcttttgcac
     3541 atacatttaa aataccattg acagaataat acaatgtaat gattgccatc agtaaaagca
     3601 gccaagacac tggaagtatc aaaagcaact tcaccccaaa aatgctgcca acgacgctgc
     3661 cgatagcaag caatttatag gtttttaaat aataaacaat ctcttgccaa aaaccttttt
     3721 tgttattgct gcatagaacc aacaagctca ttaacaggct tggtaatgcc accaaggcaa
     3781 caaccttaga caatggcatg ttaaaagcca atgcggttgt accgagcatc ggaaatccca
     3841 tgcctgtaat tccgtgcagt attgcggcag caacaaaaac gatagattgc attatttctt
     3901 gcataaccgt cctttataca gtaattcttg ctctaatata atgcctgttt ttatccgtat
     3961 aatcagttcc ggcatgcaag ccattgacat tgtcaataat aatcaaacca tcatcttgtg
     4021 cagaaaattc ttgaatatca ctcgcatttt ttataatctt tacaagataa tcaagtgcaa
     4081 gtaccatttc ctttgatact ttatttctat tttcttcaat acctttatag atacaatcac
     4141 ttctaaatct aatcatttga gtattgaccg ataagatatt gccccatctc acaccttgtt
     4201 ttttatcaaa tgatgttggt gttttaaatg gatataaatt gcccgttaat gtttttaagt
     4261 gttttttacc agtttctgtt ttagataact gattgacaat atctgatgaa cttaaaaata
     4321 gggaattacc tccaccattg gctgatttta ctacatacat aaccaaataa ctttccgggt
     4381 tttcactaaa agatgaatca ctatgaagtg gacattctcc aacatcttcc gagaaagtta
     4441 tatcattatt tatagtggat taacaaaaac cagtacggcg ttgcctcgcc ttagctcaaa
     4501 gagaacgatt ctctaaggtg ctgaagcacc aagtgaatcg gttccgtact atctgtactg
     4561 tctgcagctt cgtcgccttg tcctgatttt tgttaatcca ctatattaca taatcatcgc
     4621 ctcaatattt aatatcccaa aatatagatt taaaaccatc gcctttgtgg tttgtcagca
     4681 ttcctaattg agatagaaag tcaaataatt tcttgtttct ccggctgcca tcaagatggt
     4741 ttagattaag acctcgaaca atacaatacc caatactctg tactgtattg tgaatttctt
     4801 ctatcaaact attattgttt gcagataaaa tatacttttg attcatatag tgaattaaat
     4861 ttaaaccagt acggcgttgg cttgccttgc cgtactattt gtactgtctg cggcttcgcc
     4921 gccttgtcct gatttaaatt taatccacta taacatatcc ctgtcgtgaa atgctgtctg
     4981 aaagggtttg cctgccctta cggcagcagc cttgccctga acatcgcctc cttcatttgg
     5041 cgcacggctt cgggcagtcc gaggaagagg gcttgggcaa tcagcgaatg cccgatgttc
     5101 agttcgcgga tggcgaggat ttgggcgatg ggggtaacgt tgtgtatggt cagtccgtgt
     5161 ccggcgttga cgaccaagcc caaatcgccg gcaaaatgcg cgccgttttg cagacgctcg
     5221 aattgcttga gttgttcggc gtggctgcgc gcgtcggcat acgcgcctgt gtgcagctcg
     5281 acaacgggcg cgccgacatc ataggcggct tggatttgcc tgtcgtcggc atcgataaag
     5341 agagagactc ggatgcctgc gtcggtcagg attttggtga acccggcgat tttttcctgt
     5401 tgcgccaata cgtccaagcc gccttcggtc gtgatttcct gacgtttttc aggcacgatg
     5461 cacacgtctt ccggcatcac tttaagcgcg ttttcgagca tttcttccgt caacgccatt
     5521 tcaaggttca ggcgcgtgcg gatggcgttt ttgacggcaa acacatccgc gtctttgatg
     5581 tggcggcggt cttcgcgcag gtgcatggta atcaaatccg caccgtgcgt ttcggcaacc
     5641 agtgccgcct ccacggggct gggataagtc gtgccgcgcg cattgcggac ggtagcgatg
     5701 tggtcgatgt tgacacctaa aagcataatc tttcctttta tttctgcctt cagacggcat
     5761 ttgaagccgt gccgtccgaa gtcgggacgg tttcccgggc ggtttctttg cggtcaaact
     5821 gccgtatctg ttccaacacc tgccgcgatt tcagcccctc gggcaacagg tggcggataa
     5881 aaagccgtgt gattttcaat gcctgttgca ggctttcggc ggtgcggaaa ctgccttcgc
     5941 gcaaatcgat caggctttgc ccttcgacaa ttgcgccggc ttcgtgcgaa agcgcgtctg
     6001 cgtcctcgca tacgggcatg accgcctctt cgggcatcag ccggtaggtt ttgtccgcca
     6061 aaatatcgcc gcccgtgccg tcggcgtgca aatcgggggc aacgcccaac aggttcagca
     6121 gccgccactc gaaacggcgc aagtcgtcga tataagcggc tttgcagcac accgcctcca
     6181 tcacttccgc caacgcgtcg tataactcgg gcaacggatc ttcgcggacg gtcaacttca
     6241 gcatcaactc gttcacatac aatccgctga acaaagccct accctgcggc tgcggccacc
     6301 cgcctatcca ttcggcgcga tgtagggtct tcaactcttg cgaaccatac caagacgcac
     6361 tcaccggcac aaacggcacc aacacgccgc gcaattcgct ctgccgtttg cgcgcgcttc
     6421 ttgccaaaag tgccacacgc ccgtaacggc ggctgaacgc ttcgacccgc aggctgcttt
     6481 cgcgccaggg cgaggaagcc aacaggaaga cgggttcgtg gttgactcgg tgttcggaca
     6541 tcatgcctga aatgccgtct gaacgctgcc gccgctacaa aacggcggtc gggtacgaac
     6601 cgatgacttt gacgaacgaa gcgcgttcgc ccaagcgttc caatgccgtc tgaatctgcg
     6661 cgtcccggcg gtgtccttcg atgtcgatga agaacaggta ttcccacaaa acggatttac
     6721 tcggacggct ctcaaacttg gtcatggaaa tacccgactc agtcagcggt tgcagcagcg
     6781 aggcaaccgc gcctgcccgg ttgggcgcgg aaacggccag cgaagtcttg tcgctgccgc
     6841 ttgcaccggt ttcgtgatgt cccatcacca agaagcgcgt ggtgttgttc ggttcgtctt
     6901 cgatgcactc ggcaaccata tcgagtccgt agatttccgc cgccgtgcgt ccggcgatgg
     6961 cggcaaccgt accgtcgtcc gattcggcaa ccagccttgc ggcttcggca ttgctggaca
     7021 cggcaatccg ttcggcgttg ggcaggtgtc tgcccaacca gtcgttgcac tgcgccaacg
     7081 cctgcgcgtg ggaaaagact ttggcaatgc cttcggtgct gccgttgttt ttacgcaaaa
     7141 ggttgtggtg gatgcgcaaa acgacttcgc cgcacgcctg caacgcggta acggcaagca
     7201 aatccaacgt gcgcccgacc gagccttcgg tcgagttttc cacgggcgcg actagataat
     7261 ccgcctgacg ggtttcaacc tgcttgaagc agttgtctat ggtcggacac gccatggtgt
     7321 gcgcggcgtg tccgaaatgc ttgatcgccg cctgctgggt aaacgtgccc tgcggcccca
     7381 gataggcgat ggtcagcggg cgttctacgg cgaggcactc gctcatcact tcccgaaaca
     7441 ggcgtgctac cgattcgtcg ggcagcgggc ctttgttcaa atcctgaatg cggcgcaaca
     7501 cggcgacttc gcgttcggga cggtacactg cgcccgtgcc tttcagttcg ccgatggcgt
     7561 gtgcgtgttg cgcgcgttcg ttgagcaggc gcaggatttc cgcatcaatg gtgtcgatgg
     7621 cgttgcggtg gggaaggagg agttcgtcga tagtttggga cataatttcg cctgaacagt
     7681 aatttatcgt gcattctaac atcaaatgcc gtctgaagcg gtttcagacg gcattttccg
     7741 ttaaaggtat tcgctctgtt cgttttcctt ccacgcccgg tgcgaacaca gccacagaag
     7801 cgcaatgccg caagaggtgg acgccgccat cgttgccgcc atcacggttg ccgaaccgtc
     7861 gtgcaagaag gtcgccgcca tgcccacgcc cgcgccgatt aaagattgga atacacccaa
     7921 cacggcgttc gcgctgccgc cctcttcttt gaaataggac ataaaacacg cctgcgtgtt
     7981 tgcaccgacc aagccctgcg taccgacgga aaacatcacg cacgcaacca gcagccaaaa
     8041 cgggggcaac ccgaaaaaca gcacggcggc gaattgggac aggttggcgg caaactggac
     8101 gacaatcccc caacgcagga tgctttgcgg atgcgcgccg gttttaagcc gccacgcggt
     8161 aatacggttg aaaaacatca tcgtgatgat gttgagcgca aacgcccaag cgtattgatg
     8221 aggcgtaacg cggtaaagct gctggtacac gaaggaagat tcggtcagaa aggcgaacat
     8281 cgaaccgaag ctgaatgcct gaaaaaacag ataacccatc gcggcacggg ttttcaatac
     8341 gcgcttgaac cgccccgcca ccagcccgaa cacgtcccgt ccaattttgc cgccgacagc
     8401 aggcttgggc aggaaatgct gcaccaaacc gagcagcacc agcgaatacg ccgccagaaa
     8461 aacaaaaatc gcctgccagc cacccaagcc ctgcaacaat gcgccgacca tgggtgcgac
     8521 cagcggcaca accatcaaaa tgatgccgat aagggcaaac atctgggcgg ctttgcgtcc
     8581 ggaataataa tcgcgcacca ttgcgccgac aatgacgaca gtcatgcccg caccgaatgc
     8641 ctgcacgacg cgcagattga gaagctgttc ggcactcgaa gcaaatacga tggcggcaac
     8701 ggcaaggcaa tatacaatca gaccggtcag ggcgacgggt ttgcgcccct tgatgtcgga
     8761 caccgaaccg ccgaccacct gtccgaacgc cgtgccgaac ataaacaaac tcaggctctg
     8821 ttcgatgcgg tggacatccg cacccagcga ctgcgccatt tcgggaatcg cgggcaggta
     8881 ggcatcgatg gaaaacggca tcagcgtaac cagcatcgcc atcaaaaccg ccatcagttt
     8941 ttcgctcatt tcaggataat gggcagaagg cataaattct cgtccgattc gcaaacaaac
     9001 cgcccggcgg cggaaaacac aatacgcgga aacggaaaat tttaacacac cggcacaaac
     9061 aggcggcaac aaaagccccg gcaaaaccgg acgaaccgga acgatatagt ggattaacaa
     9121 aaaccagtac agcgttgcct tgccttagct caaagagaac gattctctaa ggtgcttaag
     9181 caccaagtga atcggttccg tactatctgt actgtctgcg gctcgccgcc ttgtcctgat
     9241 ttttgttaat ccactataaa atcccgtcat tcccgcgcag gcgggaatcc ggacatccaa
     9301 cgctgcagca atttgtcgga aataaccgaa actcaaaaaa ccggatgccc gcctgcgcgg
     9361 gaatgacgac gcaaagatgg tcgttatttc ggataaatat ccgtcttttc gttccggata
     9421 cccgctttcg caggaatgga gatggaaaga tttcagccta ttcggcaatc atcaaatacc
     9481 gcttcccaac tttatttttg atgcgacagc cggccggcaa ccaaggtggc ggcgatgacg
     9541 acaaacacgc ccaaggcgga cacgggcgac aggtgttcgc ccaaaatcaa aaccgccagc
     9601 agcacgccga cgacgggttc gagcgaaatc aacagtcccg aaacgttggc aggaacacgg
     9661 ctcatcccct tgttccacag ccaataggcg taccagccgc accccaaacc caaatacagc
     9721 agcgacaata ccatcccgac gctccagtcc acggtataac tttgcgccaa ggcaagcgaa
     9781 aacggcaggc acatcaacga tgcggcggca atggaaacag atgtgaatgc cggtgcgccg
     9841 atgcgtgcaa tcagcctttg cgtcggacgc atagcggcac aaaagcccgc gcccgccaac
     9901 aacaccagca ggcagccgaa ccagccgact tcgccgccct cttccgcacc gcccgccatc
     9961 agcagcgcga caccggcaaa tgccgccgcg ccgcatatcc agtggtaggc acgcgctttg
    10021 tcgttgaaga aaaagtgtcc gacaaacacc atcagcagcg gctcgagtcc gacaatgacc
    10081 gatgcgctgg cggcggaagt gtatttcaac ccgacaaact gaagcagcag ggtcagcaca
    10141 tagttgacga acgacacaat cagcaacggc ttccattcct cacgcggaat cttgccgaca
    10201 tgacggcggc aggcgggcag tgcaggcagc gcagcaatca gcaggcgcac gccgaccatc
    10261 aatgcgggat cgatgccgcc atagacatat ttggcggcaa taaacgagct gctccagata
    10321 atcagggcaa ggatttggta aaacatagtg agtgataaag tcagatggtc atacaaaagt
    10381 atagcggatg aacaaaaatc aggacaaggc gacgaagctg cagacagtac aaatcgtacg
    10441 gaaccgattc acttggtgct tcagcacctt agagaatcgt tctctttgag ctaaggcgag
    10501 gcaacgccgt actggttttt gttcatccac tataacagca acc
//
LOCUS       DAWFJR010000065        10501 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWFJR010000065 DAWFJR010000000
VERSION     DAWFJR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10501
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.065"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(106..420)
                     /locus_tag="WGR04_001493"
     CDS             complement(106..420)
                     /locus_tag="WGR04_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ0795024.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(431..2116)
                     /gene="rpsA"
                     /locus_tag="WGR04_001494"
     CDS             complement(431..2116)
                     /gene="rpsA"
                     /locus_tag="WGR04_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ0795025.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGTVKSVDAKGAVVALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            complement(2272..2928)
                     /locus_tag="WGR04_001495"
     CDS             complement(2272..2928)
                     /locus_tag="WGR04_001495"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ0795026.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVEWHDEENVSALAEKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYREV
                     "
     gene            complement(3172..3981)
                     /locus_tag="WGR04_001496"
     CDS             complement(3172..3981)
                     /locus_tag="WGR04_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0795027.1"
                     /translation="MPLLFLFLIAMVVRNVTLPGAMEGVAFYLKPDFSKITAELFVFV
                     LGQVFFALSLGFGVMITLSSYLDKNENLVQTAVITAITNTIIAVLAGFMIFPSLFSFG
                     VAPDSGPTLVFQSLPIVFSHMWAGPVFAVIFFSLLLIAALTTSLTIYEVLITTIQEKT
                     KIRRTAAITIVLAAIYVFGNIPSILSYGPWKDVSVFGKNIFDAFDYISGNILFMLTAL
                     GSALFVGFVMKDEAKEELLYKGNHTTVNIWFAYVKYLVPLVILLIFVSNLF"
     gene            complement(4041..4541)
                     /locus_tag="WGR04_001497"
     CDS             complement(4041..4541)
                     /locus_tag="WGR04_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0795028.1"
                     /translation="MSSPSNTNRQTWSSRLTYILTVAGATVGFGATWRFPYLVGENGG
                     GAYVFLFCIAMLVIGIPMILVENVIGRRKGVNALDAFGGPMNGKPIAKIWKLVGWMGL
                     LGTFGIMAYYMALGGWVISYIVNIIGGNLNISSPIDGVVTKGFFAEHIETALGKLRFI
                     RCFLSP"
     gene            4810..4916
                     /locus_tag="WGR04_001498"
                     /pseudo
     CDS             4810..4916
                     /locus_tag="WGR04_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(5118..5810)
                     /locus_tag="WGR04_001499"
     CDS             complement(5118..5810)
                     /locus_tag="WGR04_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEZ0795029.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIRGGYVSINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMIAAAGNRVLHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            complement(5878..7854)
                     /locus_tag="WGR04_001500"
     CDS             complement(5878..7854)
                     /locus_tag="WGR04_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0795030.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            8032..8697
                     /locus_tag="WGR04_001501"
     CDS             8032..8697
                     /locus_tag="WGR04_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ0795031.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGIPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRQLADVGFDIEFGKFMVYLPPVS
                     SIGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPEWDGYLGGKALAAGAAR
                     VAD"
     gene            8749..9576
                     /gene="mutM"
                     /locus_tag="WGR04_001502"
     CDS             8749..9576
                     /gene="mutM"
                     /locus_tag="WGR04_001502"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ0795032.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCTDYLYVRLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPYRPANRLKKKECALLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            9664..10431
                     /locus_tag="WGR04_001503"
     CDS             9664..10431
                     /locus_tag="WGR04_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0795033.1"
                     /translation="MSSNKASFFTRLRRLCRLAVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
BASE COUNT         2315 a         2380 c         3057 g         2749 t
ORIGIN      
        1 tccacgctct aatccgttgc ttgcttggct caaggtattg aagcagcgga tgaagttaaa
       61 ccacagaaaa aacgccgcgt aacagcggcg ttttgatgct aaggtttaat tggcattttc
      121 ttttaaagcc aagtcgaccc gctcgcgcaa ttctttaccg ggcttgaagt ggggtacatg
      181 tttttcaggt acttccacac gctcgccggt tttgggattg cgaccgatgc gggcaggacg
      241 atggttcaaa tcgaagctgc cgaaaccgcg gatttcgatg cgttgacctc gggcaagcga
      301 tctagtcatg gtgtcaacca agacttttac gctgtactct acgtcttttg ccagaagatg
      361 cgtgccgttt ttggcggcaa acacttctgc caaacgaacc attaactcag actttgtcat
      421 gtctgcaacc ttattcttgt tcgccggaga gtttggcttt cagcaggtca cccaagctgg
      481 tggtgccggc attcgcattg gcggcggcat tgacggagtt cagtgcttcg cggctttctt
      541 tggcatcttt ggctttaacg gaaagtttga tgctgcggtt tttgcggtca acggtaacga
      601 tgacggcttc aacttcgtcg ccttctttca gtttggtggt taaatcttca acgcggtcgg
      661 ctgcaaattc ggaagcaggc aggtagcctt ctacttcgtc agacagggcg acaacggcgc
      721 ctttcgcatc gacagatttc acagtgcctt taaccaaaga gcctttgtcg ttcacgctga
      781 tgaagttgcc gaacggatcg ccttccagtt gtttgatacc caaggagatg cgttcttttt
      841 ccacgtcgat tgccaatacg acggcttcga cttcttcgcc ttttttgtat ttgcgtacgg
      901 cttcttcgcc ggattcggtc caggacaggt cggacaggtg aaccaaaccg tcgatgccgc
      961 cgggcaggcc gacgaatacg ccgaaatcgg taatggattt aaccgcgccg gagattttgt
     1021 cgcctttgtt gtggttggcg gcaaattctt cccaaggatt ggcttggcat tgtttcatac
     1081 ccaaagagat acggcggcga ccttcgtcga tttccaaaat catgacttcg acttcgtcgc
     1141 ccagttgtac gactttgctc gggtgtacgt ttttgttggt ccagtccatt tcggagacgt
     1201 gtaccaaacc ttcgatgcct tgttcgattt cgacaaatgc gccgtagtcg gtcaggttgg
     1261 atactttgcc gaacaggcgg gtgccttgag gataacggcg ggtcagaccg ctccaaggat
     1321 cttcgcccag ttgtttcata cccaaggaaa cgcgttgttt ttcttggtcg aatttcaata
     1381 ctttggcttc aacttcctga ccgacttcca agacttcgct cgggtgtttc acgcgccgcc
     1441 atgccaaatc ggtgatgtgc aacagaccgt cgatgccgcc caagtcaacg aatgcaccgt
     1501 aatcggtaat gtttttaacg atgcctttga tgacggagcc ttcttgcagg ttttccagca
     1561 gggctttgcg ttcttcaccc aaagtagctt ccagaacagc gcggcgggac acaacaacgt
     1621 tgttgcgttt tttgtccagt ttgatcactt tgaattcgat ctctttgcct tcaaagtggg
     1681 aagtgtcttt aacgggacgt acgtcgacca aagaacccgg caggaatgcg cggatgctgc
     1741 tgatcataac ggtcaggccg cctttgactt tgccgttgat gatgccggac aggatgttgc
     1801 cgttttccat ggcctcttcc agggcaatcc aatcggcagc gcgtttggct ttttcgcggg
     1861 acagtttggt ttcgccgaag ccgttttcga cggattcgat ggtaacggta acgaagtcgc
     1921 cgactttaac ttcaatttcg ccttgagcgt ttttgaattc agctacatca atcagggatt
     1981 ctgatttcag acctgcgttt acggtaacga agttttggtc gattgccact acttcagcgg
     2041 taatcacctc acccgggttc atttcttgca gggtaaagct ttcttccaac agctgagcaa
     2101 aattttccat agacatatat aactcttttc ggtacaccgc caaggggtgc ggggtgggtt
     2161 ggtggatatc tgccgtcctt ggcagggcag acgggtacat aaggtttcag acggcatatg
     2221 ggggattttt atgccgtctg aaacgtaatt tgtgcgatta tacctgaaaa tttaaacttc
     2281 acgataccaa tcaagcactt tttttacagt ttcttctata gtcaggcggc ttgtgtccaa
     2341 aagcagggca tcgggctgtt gtttcagggg ggcaactttg cggtttcggt ctgcctcgtc
     2401 tctggcttcg atgtcggaca ggatgcgctc gaatgccaaa ccttcgcagg ggatgccgat
     2461 ttgtttggcg cggcgttcgg ccctgatttt ggattctgct gtcagaaaaa ttttgagttc
     2521 ggcctggggg aagacgaccg atccggtatc ccgtccgtcg gcaaccagtc ctttttcggt
     2581 cagaaaatcg cgttggcgtt gcagcagtgc ggcgcggact ttaggcaact gtgcgactgc
     2641 ggatgcgccc atgccgatgg cttctgtccg gatgccgtct gaaacgtctt cgccgccgag
     2701 caggatgcgg ctgcctgaaa atacggcggg cagtttttcg gcaagtgcgg aaacattttc
     2761 ttcatcgtgc cattccactc cttgtttttg tgcatatagg gcggtcaggc ggtagagcgc
     2821 gccggtatcg agataatcgt atcccaatgc ggcggcgacg cgggcggcga ccgtgccttt
     2881 gcccgatgcg cccgggccgt cgatggcgat gactttttgt ctgttcataa tggggattcc
     2941 tgatggtttg gggtatgggt tttgccgtct gaaggatgtg tttcccgttg gggcggattc
     3001 tacctgtttt aaaggacgat tgtctaagca gacggaacgc cgcctgcccg gaaatatccg
     3061 acagaagccg gcaagccggt tcggattccg gcgttgcggg tattgtggcg gacatcaatg
     3121 caatgccgtc tgaaagaggt atgaccttca gacggcatcg attgctgcgg attaaaacag
     3181 gttgctgacg aaaatcagca aaatcaccag cggcacgagg tatttcacat aggcaaacca
     3241 aatattgacc gtcgtatggt tgcctttata aagcaattct tccttcgctt catccttcat
     3301 cacgaaaccg acaaacaatg ccgagccaag cgcggtcagc ataaacaaga tgttgccgct
     3361 gatgtagtcg aaggcatcga aaatattttt gccgaacacg gaaacgtctt tccacgggcc
     3421 atagctcaaa atagacggga tgttgccgaa aacgtagatg gcggccaata caatcgtgat
     3481 tgcggcggta cggcggattt tggttttttc ctgaatggtc gtaatcaaca cttcataaat
     3541 cgtcagcgaa gttgtcagag cggcaatcag gagcagagag aagaaaatca cggcgaacac
     3601 aggtcccgcc cacatatgtg agaacacaat cggcaagctt tggaacacca aagtcggacc
     3661 ggaatcgggg gcaacgccga agctgaagag cgacgggaaa atcataaagc ccgcaagtac
     3721 ggcgatgatg gtattggtaa ttgccgtgat aactgcggtt tgcaccagat tttcgttttt
     3781 atccaaatag ctggacaagg taatcatcac gccgaaaccc aggctcaggg caaaaaatac
     3841 ctgccccaaa acgaagacga acagttcggc ggtaatcttg ctgaaatcag gtttcagata
     3901 gaaagcaacc ccttccattg cgcccggaag ggtaacgttg cggacgacca tcgcgattag
     3961 gaacaaaaat agcagcggca tcagctattt tgccgctttt tcaatgccgc cgataacgcc
     4021 tttgaccaaa atccattggt tcacggcgac aaaaagcagc gtataaaacg caatttccca
     4081 agggctgttt caatgtgttc ggcaaagaag ccttttgtaa ccacaccgtc gatggggctg
     4141 gaaatattca aatttcctcc aataatatta acgatatagc tgattaccca gccgccgagt
     4201 gccatgtaat aagccatgat gccgaacgtg ccgagcagcc ccatccagcc gaccagtttc
     4261 caaattttag caatgggttt gccgttcatc ggaccgccga acgcatccag cgcgttcacg
     4321 cctttgcgcc gtccgatgac attttccacc aaaatcatcg gaatgccgat aaccagcatc
     4381 gcgatacaga ataaaaacac atacgcgcca ccaccgtttt caccgaccaa atacgggaaa
     4441 cgccacgtcg cgccgaaacc gacagtcgcg ccggcaacgg tcaggatata ggttaatcgg
     4501 ctggaccaag tttgacgatt ggtatttgaa ggggaagaca tattgaaacc gtgtccgatt
     4561 gagataaagc ggaaattcta cacgcgtttt ttaacaggaa ctgactgatt gcttttcaac
     4621 tgtctagtga ttttccatgt aaaaggcata tatttcagtg agtatttctg atgagtataa
     4681 cccgatgagg aagaatcgga gtttataaat agattaattt gttattcttc tacatcggtg
     4741 tatggaaatg aaattttgtt aattatatta atgatagcat ttaaataatg atgaaagaga
     4801 gggaatttga aatatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag
     4861 tacaaatagt acggcaaggc gaggcaacgc tgtactggtt taaatttaat ccactataaa
     4921 aaaaccatct atacaagggg agaagtatag atggcaaaac acattacggg gaaaacgtct
     4981 tactcataag cctgcttgaa caggcgttac tcagacaaat ggattatatc gtaaattgat
     5041 tttttgcgtt aaattgggtt aaaccatata tttaatgagt atgtggatga atataaatgg
     5101 aattcttgga tgttatttca gaattttgga cattcgataa atttccattc cccgggtttg
     5161 aggttttctg tttccaaatg tgcgaatcgc cgacgatgaa ggtgtagcac gcggttgccg
     5221 gcggcggcga tcatgcgttt gacttggtgg tattttcctt cggtaatggt cagcagcagg
     5281 gtggtcgggt tttccaaaac ggcatcggcg gcacaaacgg tttcgttttc gtcgtggagc
     5341 agcacgccgt ttttcaaggt ttcgcagagc gtttctcctg tggggtgttt gagcgttact
     5401 tcgtatagct tgggaatttt tctgctcggc gaagtcaggt tgtggttcag tttgccgtcg
     5461 ttggtaatca gcaatacgcc ggtcgtatct gcatccagcc tgccgaccgc ctgcatatcg
     5521 atgtttcgca tattgtcggg gaacaggctg aatacgctgc ggtagtgctt gggtttgtgc
     5581 gaagtttcgt aatcttcagg cttgttgagc atgatgtaga aatagggttc gggaacgacg
     5641 gttactgctt ccccgtcaat atccaacgtt tcgacggatg aggaatcgat gtctgcatcg
     5701 gtgtcgtcca tacaggttcc gttgatggag acgtagccgc ccctaatcag ccattggcac
     5761 tgctttcggc ttcctatgcc ttgatattgc aggtatttga taagtttcat gatggtatgg
     5821 gaaatgtggg atgaaaaaga caggactgag aaagtcctgt catgtgccgt atccgaatca
     5881 gcttccaatc agtttgaccc tctgtcccgg attgatggtg ttcaggttgg gattgagccg
     5941 gcggatgtcg tcgatatgga tattgaagcg cgcggcgata cttttgaagg tgtcgccttt
     6001 gcgcgcggtg taggatactt tttcaatacg ggtttgggca ggggctgcct gcgccaggcg
     6061 gagtacctgt cctttttgga tggtgttgcc tttgatgttg ttggcgacaa tcaggtcggc
     6121 tacgctgacg ttgtagcgtt tggcaatgtt gaacagggtg tcgccttcta caacgcggtg
     6181 gatgctggca tggagcgggg agatttcggc ttcgccgtcg gcagctttgg ctacgcgcgt
     6241 ttccaaacgt gcccggcgtt tgccgtcggc ggcaacggta tgttgcggag atgaggaaat
     6301 gttgtgttcg gcaactgtgt caggcgtgcc gatgacggcg gagatggttt cttcagcctg
     6361 tcggcgcagg ttgtttcggg caacaagctg catgagttcg tctgccgcct gcatgtcgtt
     6421 ttgggggatg atctgcgcga gtgttgcggt ttgggtttca gacggcatgg cggtctgttt
     6481 ttcggtttga ggttgcgctg cggctgtcgc aagggcaggt tcggcaatac ggacaagggg
     6541 atcgggttcc gtacggacgg ttttctgcgg caaaggtgcg acggtaatgt ccgctgtctg
     6601 tgcggcggcg ggtcggattc gggcaatgcc gacgttcacc gtgcctgccg gcatattgga
     6661 acggtaggtg tcgggcgtat tgtcgatgtc gatggaaacg acggattccg atgccgtctg
     6721 aagggttttg ccgttcttgg cgacaaggat gctgcgtcct gcgttgacaa ggttgccgtt
     6781 caggttgttg aggcgtttga tgtcggcaat gctcatgccg gttgccgtcg agatgtcgga
     6841 caggctggtt ttggcggcag gcgtatagac ttcccatgaa aacaggctgt cgggtgcggc
     6901 gttgaggtag ttgctttgga aggtttgtac ggacgcgaca ggaagcagca gtttgcgttt
     6961 gcttttgggg ataaacgcgg ggacgttgaa tgcggggttt agggcgagca gctcgctttg
     7021 cgtgatgccg gcaagccggg cgatggcttc gttgtcgagc ggacgatccg gttcgactgc
     7081 ctgaaaatag ggtttgttgt ctatgtcgct gatattcatg ccgaaagatt ggggagtggc
     7141 aatgatgttg cgcacggcga gcagcttggg gacatagttg cgcgtttcgt tgggcatacg
     7201 caggttttcg taggtcggtt cgagcccttg ggcgcgggcg cggttgatgg cgcgtccgac
     7261 gttgccttca ccccagttgt aggcggcaaa ggcaagcggc cagtcgccga acagtccata
     7321 gaggtattgc agatagttga gtgcggcatc ggtggcggcg taaacgtcgt gcctgccgtc
     7381 gtaaaccggt gttttttcca ggccgtaatg cctgccggta gcgggcataa actgccataa
     7441 tcctgatgcg ccgacgtgtg atttggcttt ggtgacgaac gcgctttcga tgaagggaag
     7501 cagggcggct tcggcgggca tattgcgttt tttgacttcg ttggcgatat ggtacatata
     7561 gggtctactc cggttgatga ccctgttgaa atagctgtgg cttgcgatga atttgctttc
     7621 gtggcggcgt accagttcgg gattgacttc gcccatccgg aagccttggc gcagctcgct
     7681 ccacaggctg ccggattgga aatattgttt tgtcggtggc aggtcgagta ttgaagagtt
     7741 taagcgcata atcgccaaac cgatttggtg tgatgaggtg ttttgggcgt ataggaaacc
     7801 cggacaaacg gacagacctg acgcggtcag ggcgatggtt ttgagtttgg acatgattta
     7861 tatcggcgga ataaacatat tttatcgtcc gatggtactg tttgactgaa aaagcgtcaa
     7921 gttttacagg aactttatgc ggattcggaa atgggcggcc gatgtgtttt ttgacagaaa
     7981 tcctgtttgg aatagaatat cggcaggcaa tctgtttgaa acggacgcaa tatggatgca
     8041 tggtttgaag atacggcgat ggggcggtat gttgcaaaat tggaacagga tttcttcggg
     8101 cggtatctag attcataccg tttttcggga atgtgtgcgg ttcaggtggg cggtccgtgg
     8161 ctgagcctgt ctgaagatgt tgtctgtgtg ccgcgagata tgtcgatgtc ggcggagaat
     8221 atggctttgg cggatgtttc tgcggatatg ctgctttttc cgcatacgct tgaaggcggt
     8281 atcccttcgc aaatcctgtc cgaggcgcac cggatattga aaccgtgcgg acgcttgatg
     8341 ctgacgggtt tcaatccgta ttcgctctgg ggattcagcc gttggtttga cggcgaacgc
     8401 ctgccggaaa aacggttttg tctgccgctg cccgagttga aaaggcagct tgcggatgtc
     8461 ggtttcgata ttgaatttgg gaaatttatg gtgtatctgc cgcccgtttc gtcgatcggg
     8521 caaatacgct tttggcggtt tatggaaaag gcgggcgacc gttggtggcc gcaatgtgcc
     8581 gcagtgtacg gtttggtttt ggtcaaaagg gcggcgggcg taacgcccct gcccgagtgg
     8641 gacgggtatt tgggcggcaa agcccttgcg gcgggtgcgg caagggttgc ggattaggga
     8701 cagccgtctt tcagacggca tctgtcagaa gaaatgtcgg gaggggttat gccggaattg
     8761 ccggaagtgg aaacgacgtt gcgcggcatc gcgccgcata ttgaagggaa aacggtggaa
     8821 gccgtggtat tgcgccaatt gaagctgcgc tggcagatca atcccgattt gggggagatt
     8881 ttgtccggtc ggcaggtttt gtcctgcggc aggcgtgcca aatatctgat tgtccgtttc
     8941 caaacgggga ttttgctgat tcacttgggg atgtcgggca gcttgcggat ttttacgccg
     9001 tcggacggac gtatcggcag gccggacaga cacgatcacg tcgatattgt gttttcagac
     9061 ggcacggtca tgcgttaccg cgatccgaga aagttcggcg cgatactttg gtatgaggga
     9121 atcgaagaac atcatccgct gttggaaaaa ctggggccgg agcctttgtc ggaggcattt
     9181 tgcacggatt atctgtatgt gaggctgaag gcgcagaagc gcgcggtcaa acttgccctg
     9241 atggacaatg cggtcgtggt cggtgtgggc aacatttatg ccaacgagag cctgtttagg
     9301 gcgggcattt cgccctaccg tcctgccaac cgcctgaaaa agaaagagtg cgcacttttg
     9361 gttgaaaccg tcaaagcggt gttgcagcgc gccattgaaa cgggcggcag taccttgagg
     9421 gattttgtgg acagcgacgg caaaagcggc tacttccaac aggaatatac agtgtacggg
     9481 cggcacaatc agccgtgccc ccagtgcggc ggtttggttg tgaaagaaac tttggggcag
     9541 cgcggcacgt tttattgccc gaactgtcag aaataggact gaaaacggtt tcagacggca
     9601 ttttatcggt atgccgtccg aacgtttcaa caacaaacac cgattatcgg gaaagaattg
     9661 ctcatgtctt caaataaagc ttcatttttt acacgtctgc gccgcttgtg ccgtttggcg
     9721 gtctggctgt tcaaaaccgg gaaaaacctg cgcggtattg acggcggttg ccccgagtcg
     9781 cgcaatcggg cggtaatcga gttgggcagg ggggttttgg cggctttgga tatcggattg
     9841 gaggtgggca gacccgcacc cgaacatccg aacggtgtct tggttgccgc caaccacgtg
     9901 tcctggctgg atattttcgc gatgagcgcg gtttatccga gcagctttat cgccaagcag
     9961 gaaatcaaaa gctggccggt attgggcaag atggggcaga acgcgggaac ggtgttcatc
    10021 aaccgcaatt cgcggcgcga catcgaaccg attaaccgcg ccgtctgcga aaccttgcaa
    10081 cgcggtcaaa acgtcagttt tttccccgaa gcgcggactt cctccggatt ggggcttttg
    10141 ccgttcaaag ccgcgctgtt ccaatccgcc atcgatgcgg gggcaaaggt tttggcggtc
    10201 gcgctgcgtt attatgacga aacgggaaaa aggacggctc gtccctcata tgccgatgtc
    10261 ggtttgccga cctgcctgtg gcgcatcgtg tctatgaaaa aattgacgat aaaagtcgat
    10321 ttcgtttgcg tggcggatgc ggcggaaagc gaagaccgtt atgctttaaa agataaaatc
    10381 gaagaaagca tccgtgccgt tgtcgccgac gatgcggata tcgctgtctg aaaccggttg
    10441 tcggaatgtg gcagtatgat tcgcttttgt ggatgtatag tggattaaca aaaaccagta
    10501 c
//
LOCUS       DAWFJR010000066        10331 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWFJR010000066 DAWFJR010000000
VERSION     DAWFJR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10331
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.066"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(75..149)
                     /locus_tag="WGR04_001504"
     tRNA            complement(75..149)
                     /locus_tag="WGR04_001504"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Glu,seq:ttc)
     gene            complement(167..243)
                     /locus_tag="WGR04_001505"
     tRNA            complement(167..243)
                     /locus_tag="WGR04_001505"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(207..209),aa:Arg,seq:acg)
     gene            complement(353..2026)
                     /gene="recN"
                     /locus_tag="WGR04_001506"
     CDS             complement(353..2026)
                     /gene="recN"
                     /locus_tag="WGR04_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ0795034.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKSVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGWSDINPNELAAQEQRMGELMGMARKYQIEPEELPAKLAEIEECLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(2210..2812)
                     /locus_tag="WGR04_001507"
     CDS             complement(2210..2812)
                     /locus_tag="WGR04_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ0795035.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVIQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLIEMPLTDEEEPEAVPSDTLPN"
     gene            complement(2913..3854)
                     /gene="ylqF"
                     /locus_tag="WGR04_001508"
     CDS             complement(2913..3854)
                     /gene="ylqF"
                     /locus_tag="WGR04_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ0795036.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGSVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3936..4790)
                     /gene="rapZ"
                     /locus_tag="WGR04_001509"
     CDS             complement(3936..4790)
                     /gene="rapZ"
                     /locus_tag="WGR04_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ0795037.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIAYLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLPSR"
     gene            complement(4771..5733)
                     /locus_tag="WGR04_001510"
     CDS             complement(4771..5733)
                     /locus_tag="WGR04_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ0795038.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            complement(5737..6186)
                     /gene="ptsN"
                     /locus_tag="WGR04_001511"
     CDS             complement(5737..6186)
                     /gene="ptsN"
                     /locus_tag="WGR04_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ0795039.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            complement(6371..7261)
                     /gene="ubiA"
                     /locus_tag="WGR04_001512"
     CDS             complement(6371..7261)
                     /gene="ubiA"
                     /locus_tag="WGR04_001512"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ0795040.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVGNSVPVEAWILFTANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            complement(7291..7767)
                     /locus_tag="WGR04_001513"
     CDS             complement(7291..7767)
                     /locus_tag="WGR04_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ0795041.1"
                     /translation="MEHLFGKWLPDLPADVSDGISLPMVRLLHTRSLTAALRPLPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7782..8429)
                     /gene="bioD"
                     /locus_tag="WGR04_001514"
     CDS             complement(7782..8429)
                     /gene="bioD"
                     /locus_tag="WGR04_001514"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ0795042.1"
                     /translation="MKGVYFVSGIDTGIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QDIAEDIAVHRKIMGIPMQEADEQRLTMPEIFSYPASPHLAARLDGRDLDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLIFNHIHDSSDECVAQDSLNYLQCRLKDSFPEAEWLELDKTDAV"
     gene            complement(8426..9727)
                     /gene="bioA"
                     /locus_tag="WGR04_001515"
     CDS             complement(8426..9727)
                     /gene="bioA"
                     /locus_tag="WGR04_001515"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ0795043.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVEAQIKQMSHVMFGGLTHDPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKSRFDDEWDEADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGLLTELADKTVQILKEHSK"
     gene            9944..10059
                     /locus_tag="WGR04_001516"
                     /pseudo
     CDS             9944..10059
                     /locus_tag="WGR04_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         2429 a         2807 c         2607 g         2488 t
ORIGIN      
        1 atgggaaatg ggaaatggga aatgggaaat gggaaatggg aaatgcctcc gatcatatcg
       61 gaggcatttt aaactggcac gcccacgggg aatcgaaccc cggttaccgc cgtgaaaggg
      121 cgatgtccta accgctagac gatgggcgca tattgtattt tcttactggc gcacccggag
      181 cgattcgaac gcccgaccct ctggttcgta gccagatact ctatccaact gagctacggg
      241 tgcagcacaa tcacttgttc aagtgattaa gaaagcgtga taatacggat ttatctgagt
      301 tctgtctatt gttttagact tttattttta gttatttttt aactgattga ttttaaaata
      361 aactattttt cgacgcaagt tgcagcaatt ctgccgcatg ttgccgcgtc gtatcggtaa
      421 tgacttctcc gcccaacata cgggcaacct cttcgatccg ttggatttca tccaatatac
      481 tgatttcgct gacggtttgc tctccctcgc tgtgcttgcg cacccgccag tggttttctc
      541 cgcaggatgc gacttgggga aggtgggtaa cggcaagcac ctgatgtttt ctgcccaacg
      601 cacgtaatgc cttgccgacc atttcagcca ctccccctcc aataccggta tcgacctcat
      661 caaaaatcag ggtgggaact tgggtatatt gactggcaac aacctgtaag gcaaggctga
      721 tacgcgccaa ttcgccgccg gaggcaactt tattcagcgg acggggcgga ttgcctttgt
      781 tggcggcaac ttgaaattga acctgctcta aaccgtgtgc cgtcggcgac gaaggcaaca
      841 ggacgatgtc gaaacgtgca cctttcatgg caaggtgttg catatgctcg gtcgtttcgc
      901 cgctcaaacg ctctgccgcc tgatggcgca tggcagaaag gatgtgggca gcttcctgat
      961 attcggcaaa attgtgggca acattatgct cgagcgcgtc caaatcggcg gcagcttgca
     1021 ggctttgcag gcactcttcg atttctgcca acttggcagg caactcttca ggttcgatct
     1081 ggtatttccg cgccatcccc atcagttcgc ccatacgctg ttcctgtgcc gccaattcat
     1141 tgggattgat gtcgctccaa cctgccacat cgcgcatatt ggcactgatt tcgcccaatt
     1201 cggcttcgat gcttgccaac atattcaggc tctcggcaaa gcgcggctcg atgttttgca
     1261 gattggccaa tagcttttga cattgataga tatggcgttg gatgccgttg tcgccgtcaa
     1321 tcttgcttcc gacttcttcg gcagcctgca acagctcggc agaatgggca aggctgtcgt
     1381 ggctttggct gagggcttcc cattcgcctt gtttaatgtc caactgattc agttcgttaa
     1441 actgccattc cagacgctcc cgctcgataa tgacggcatc ggcgtgttcc tgcgcctctt
     1501 ggagggcttt tttcgcattg gcccaatttt gataaagctg cctgacggtt tccgcctgcg
     1561 ccctgctacc cgcaaatgcg tccaacaatt cgcgctgggc ggcttcctga ttaagcgaat
     1621 gatgggcgtt ttgcccgtgg atgtcgataa gctggctacc gacggatttg agttgcgcca
     1681 aggtagcggc ctgattgttg ataaagctgc ggcttttgcc tttggcatcg ataatgcggc
     1741 ggatactgag ttcttcttct ccgtcgttta aaagcccctg ttcatacaat tctgctttta
     1801 aaacaggcaa atgggaaata tcgaacaatg ccgacaactg cgcttctttt gcaccgctgc
     1861 ggacttggct gtaatcggct ttgtcgccca acagcagacc aatcgcatcc aaagtaatgg
     1921 acttgcccgc gccggtttct ccggtcaata cggtaaagcc gctttgaaaa tccagattca
     1981 gattttcaac aatgacaaaa tcacgcaaag aaagtgttag aagcattgtg aggattcctt
     2041 agcaattcta ccgacaaaag caatatcaac tttagatata cgaattataa ctaagatttt
     2101 tttaaattaa aaataagttt tccttaattt tgcttagtac ttgtttttat ctgcttattt
     2161 gttgcaatgc cgtctgaagc aatgtgcgtt tcagacggca tttggaattt cagttgggca
     2221 gggtatcgga cggtacggct tccggctctt cttcatccgt aagcggcatt tctatcaaat
     2281 cgggcaaaac cagttcgccc agttcggtca gcggaggaag ttcttccaga ccgtccaagc
     2341 gcaaatcgct gagaaacgtt gccgttgttg cccacaatgc gggttttccc aatgtgtccc
     2401 gatgtccgat gacttcaatc cacccccgat cctgcaaggt ctggatcacg ttctgcgaca
     2461 ccgccacacc gcgtatgcct tcgatgtcgc cgcgcgttac gggctgctgg taggcgataa
     2521 tcgccagtgt ttccatcacg gcgcgggagt agcgcggcgc acgctgttct tgcaggctgc
     2581 ccagccgctc gaatgccgtc tgaacaatct gaaaacgcca gccctcttgc gtatgcacca
     2641 gttgcaacgc cctatcctgc caacgcgttt tcaactgcgc caacacatca atcagtttgt
     2701 cctgcgacag tggcggcaca cacaattcgc gcatagattt ttcggtcagc ggttcggttt
     2761 gggtcagcag tgcggcttca attagtgcgt cgggagaaat tttgtcggtc atacgggtat
     2821 ccgtgctgaa acggcatggg tttggatatg ccgtctgaaa tcggttggag tagagagaag
     2881 ctgcctgaaa aatatttttc agacggcatt ctttatgctt ccgaaggctt ctgcccttta
     2941 cgctctgctt ttctggcttc gcgtatggct ttgagttcgg cttctttctg acgggctttt
     3001 ttgagccaag tttcccattg gttcggcgtt tcgagggtga ttctgccgat tttgccttca
     3061 cggaagtcgg taaggatgtt ttcggcggct ttttggtagt tgatccgtcc gccgctgagg
     3121 actgcgccgc gttttttggc tatccattcg agccaaacgt tttcgtccca gtggctgctg
     3181 gggtctttgt cggcttggta gcgttcttgc aacataggga ggtagtggcg gcggaggtag
     3241 tctaaaagtt cgagggcgac ttcttcttcg tccaatgcgt tgcgtccgac tgaaccgccg
     3301 gcggcgaggt tgtagccgct ttcttcgaca atgattttcg gccacagcat tccgggggtg
     3361 tcgtacagcc agaagtcgtc ggcaaggaaa aggcgttgtt cggctttggt gatgccgggt
     3421 tcgttgccgg ttttggcgga ttttttgcct atcatgccgt tgatgagggt ggatttgccg
     3481 acgttgggaa tgccgcaaat caagacgcgc aagggtttgt ctatgccttg ccggtgggga
     3541 atcatcgcgc ggcaggcttg ggtgattttg ccgtgtgcgc cggtttcgga ggaatcgagg
     3601 gcaatggcgc acgtgtcggg gcggctgttg tagtggtcaa gccagacctt tgtgcgttcg
     3661 gggtcggcaa gatcttgttt gtttaggatt ttaagtttgg gtttgccttt ggaaagctgg
     3721 gcaagcaggg ggttttcgct ggaggcgggc atacgcgcgt ccagcatttc aatcaccata
     3781 tcaacgcttt ttgcacgctc ggcgatggct tttttcgcct tgttcatatg accgggaaac
     3841 cattggattg ccatatcttt cctttcttct caatatttga aatgccgtct gaaacagaaa
     3901 agcggggttt cagacggcat aattgtttaa tgggattaac ggctcggcag gttttgcgcc
     3961 tgtctgtgcc gtatcagcaa ttcataacgc cctttcaacc ttcgggcgag tttttcgaca
     4021 atatagaccg aacggtgctg tcctcccgtg caaccgatgg cgacggtaac gtagctcctg
     4081 ctttcatcct ccaaacgcgg taaccaatgc gtaacaaacc tttcgatgtc gtcaaccatt
     4141 tcctgcacaa gcggctgtcc gtccaaataa tcccaaacgg gcttgtccat accggtgtaa
     4201 ggcctcaact cgggatcgta atacgggttg ggcagactgc gcatatcgaa cataaaatcc
     4261 gcgttgttcg gcacaccgta tttgaacccg aaggactcca aaattaccag cagcccggta
     4321 cgttcgacct tcagccactg ccggactgca tggcggagct gttgggcatt catcttggaa
     4381 gtgtcgatac agtaggcgat ttccttaagc ggaaacagcc attcccgttc tttctttaag
     4441 ctttccaaca gggtcatatc ctgattgctc agaggatgtc ctcgcctggt ctcggaaaac
     4501 cggcggacca acacgctttc ttccgcctcg acaaacaaaa cttcaaccct gtgccccagt
     4561 ccgcgcagat aggcaatctg ttcccgcgcc tgtccgatgt caatgccgga acgcacatcg
     4621 acgctgaccg ccaattcggt ttcgtccgca cgctcgatat gatacgacac cagcgcgggc
     4681 aacatttcca aaggcaaatt gtccacgcag aaataacccg aatcttccat ttggcgcagt
     4741 gcgacggatt tgcccgaacc ggacaacccg ctaatcagga cgattttcat gattctgttc
     4801 gttttcttta agttgcgtct gatggcgttc caaaaattcg cgcgtactgt ccttaccgcg
     4861 caactgcaaa atgtaattgc gtaccgccgc ttcaaccaat acggcaaggt tgcgtccgac
     4921 cgcgacaggc agcgttaccg aacggacgtt gacgttgagg atggattcgg tttcggtgcg
     4981 gatactcaac cggtcgagct gcttcatata ctcgtcgtct gcctcgacca aattgataat
     5041 gagttgcagg atttttttgg ggcggatgga agtttcgccg aaaatatggc ggatattgag
     5101 tatccccaag ccgcgcactt ccaaaaaatc gcgcagcata ggcgaacacc gcccttccag
     5161 cgtttccggg ccgatgcgga acagctcgac cgcatcgtcg gcaatcaggc tgtggccgcg
     5221 cgaaatcagc tccaacgcca attcgctctt acccaaaccc gaatgtccgg taatcagcac
     5281 gccgatttca aacacatcga gaaatacgcc gtgtttgacg gacgatgccg ccagggtgcg
     5341 ttgcaggtaa atccgcaaca cgtccatcag ataggggctt tcgagtttgg aagtcagcag
     5401 tggaatatcg tttttatggc agtagtcgcg cagtcccggg gaaaccggca agccgtttgc
     5461 cacaataacc aaagacatag aaatgtcgaa caggtcgccg aactgataac ccgtttcccc
     5521 cgattcgaga cggttcagat attccgactc cgccaaaccg accacttgga tttggttggg
     5581 gtggatgaaa ttcaggtgtc cgaccaaggc gaggacgggc ttgtccgcct ctacgccgat
     5641 acggttgtcc gcacccgaat tgccggcggc ccaggcgagt tgcaacttgt attggttgtc
     5701 gtcaaacagt cggcggacag agatactggg catattttat tcttcggtca ggatggcgcg
     5761 ggcttcttcc gcagaagaaa ccgtcatcag cgattcgcgg atgctttttt gggaaaactt
     5821 gccggccagt ttggacaata cctccaaatg ctcgcccgtt gcattctcag gaaccagcaa
     5881 gataaaaacc agggaaaccg gtttgccgtc cggtgcgtca aatccgacgg gttcgcgcgt
     5941 gcggatgaac gcgcccgtcg cctgcttcac gccggcatga cggccgtgcg ggatggcaac
     6001 gccctgcccc aaaccggtcg aaccgagttt ttcacgggca aaaagacatt cgaaaacatc
     6061 agcatgggac aatgaggatt cgcgttccaa aagcaggcct gcttcctcaa acagcctttt
     6121 tttactgccc acctccatat ccaaaacaat atgggacaaa ggcaaaattt cgccgataag
     6181 gctcataagc ttctcttttc agacatcgca aaacagaaag attgtaccga ctgccggggc
     6241 aaatctcaat cccgcatacg gtacgggctg acataacaca gcgttttaaa aacatatttt
     6301 aacgcttttc ggcacagata gaaatgccgt ccaaagcagt ttacggctct tcagacggca
     6361 ttgccctgcc ttatttcgcg aaaaacgtat gggcaaaaat ggcggtaaac cacacccaac
     6421 caattctgtt gtttgccaaa aacgtttcaa aacagatttg ccggacgcgg cttttgatgg
     6481 cggcatattg gcggtattgc agcagcagga cgatggggat tgccgtccaa tatgcccaag
     6541 ccgcgccgat aaccgcaccc aatactgcca tcagcagggt aaagccgcca tgacacagca
     6601 taacagcggc gatgtcgtaa cgcccgaatg tgacggcgga ggttttgatg ccgattttca
     6661 aatcgtcttc tttgtccgcc atggcgtaaa cagtgtcgta agccagtgtc cataaaacat
     6721 tggcggtaaa aagtatccat gcttcaacgg ggacgctgtt gcccactgcg gcaaacgcca
     6781 tcgggatacc gaaggaaaag gcaagcccga gatagagttg gggaatcgga aaaaaacgtt
     6841 tggtaaacgg gtaagtcagc gcaagaaaca gcgcgggcag gctcatcagc caagtcagat
     6901 gattcagcgg aatcaggcac aatgcggcaa gcaggcacaa aaatgccgtc agcagcaggg
     6961 cttctttttt cttgaccctg ccctgtgcga acggacggtt tttagtgcgc tcgacagccc
     7021 cgtcaaaatc gcggtcggca aaatcgttga tgacgcagcc ggcactgcgc attaaaaacg
     7081 taccgattgt aaacgccgcc aataccgcca aatcgggaat gccgtctgaa gccagccaca
     7141 acgcccagta ggtcggccac agcaaaagca gcgtcccgat gggcttgtcc gcccgcatca
     7201 ggcgcaggta cacatccaaa cgatcggaca gtcgtaaaaa taaaggggat ttaggattca
     7261 tattgccgca cagcttgaaa aaacggtatt ttatccgata aagcgtttca gttcgggcag
     7321 aaaatactcg gtcagcagca tttcctcgcc gtgacgggaa aaccgagaac gccgcgcggc
     7381 aaagtaccgt ccgcatcctt cgccggaaac ggcaaactca aacgccgaac gcgccccttc
     7441 caaatcggct tgaaacagac gctcgcccaa agggcgcgtg ccgcagtcca aaatgttttg
     7501 ccaaaacgcc gaacctatcc ggcactcgct ccttgccgcg acaacaggga taccgtccag
     7561 cttcaacaaa acttcgcgca ccagcctccc tccgcattcc gtctccaatt cgcccagttt
     7621 cagcagttcc accgaaaatg tatgcggcaa ggggcgcaat gcggcggtca gcgaccgggt
     7681 gtgcagcagc cgcaccatcg gcaggctgat gccgtctgaa acgtcggcgg gcaggtcggg
     7741 cagccatttc ccaaataggt gttccatatt tttcccaatc tttataccgc gtctgtttta
     7801 tccaactcca accattccgc ctcggggaaa ctgtctttca gacggcattg cagatagttc
     7861 aggctgtctt gcgcgacaca ttcgtcggaa ctgtcgtgaa tatggttgaa aatcaggctg
     7921 tgcaggcgga tgccgtattg tttgagcgcg gcgaaactga gtaaagtgtg gttgatactg
     7981 ccaagccgtc cgctggtaac gaggatgacg ggatagcctt gctgacggat ataatcaatg
     8041 gttaacaggt tttccgtcag cggaaccatc aatccgcccg cgccttcgac caaaacgact
     8101 tcgtactgcg ccgccaattc ttgtgtggcg gtacggattt tgtccaagtc caaatccctg
     8161 ccatccagac gggcggcaag gtggggcgag gcgggatagc tgaagatttc gggcatggtc
     8221 agacgctgtt catcggcttc ctgcatgggt atgcccatga ttttgcggtg aacggcgatg
     8281 tcttcggcga tgtcttggca accggtttgc acgggctttt gcgtgatcac gcttttgccc
     8341 tgctgcaaca attgttttgc caacataccg gtggcgacag ttttgccgat gcccgtatca
     8401 atgccgctga cgaagtaaac gcctttcatt tgctgtgttc cttcaaaatt tgtacggttt
     8461 tgtcggcaag ttcggtcaag aggccgtctg aaatgatgta aggcggcatg aggtacacca
     8521 gcctgccgaa tgggcgcacc caaatgccct gcgccacgca gtccgcttga aaacgcgcca
     8581 tatccacgcc tttatccagc tcgatcacgc cgattgcacc caaaacgcgt acgtctttca
     8641 cgccgcgaat gtcccacgcg gctttcagac ggccttttaa gatgctttca atgcggcgga
     8701 tatttgcctg ccagtcttga gacaaaagca gtttgaccga agcgcaggca acggcacacg
     8761 ccagtgggtt tgccataaat gtcggtccgt gcataaacac gcccgcctcg ccgcgcgaaa
     8821 tcgtttcggt aactttttgc gaagtgattg ccgccgccag cgtcatatag ccgccgctca
     8881 aacccttgcc gatgcacata atatccggca cgacctccgc gtgttcgcag gcaaacatct
     8941 tgcccgtgcg cccgaatccg gtggcgattt catcaaaaat caaaacgata tcaaattcgt
     9001 cgcacaaatc gcgcaatccg cgaagatact gcggatgata aaaatacatg ccgcccgcgc
     9061 cctgcacgac cggctctaaa ataaaggcgg caatatccgc atgatgcact tcaaataagg
     9121 cgcggacagg ctgcaaatcc gcctcgtccc attcatcgtc gaaacggctt ttcggattat
     9181 cgacaaaata acgctgcggc aacgcgctgc cgaaaatatg gtgcatcccc gtttccggat
     9241 cgcagacgga catcgcgttc caagtatcgc cgtgataccc gcgccgtacc gtcgcaatat
     9301 tctgcttcgc cgtcaacccc cgcgcctgct ggtattgcac cgccattttc aacgcgactt
     9361 ctaccgaaac cgaaccagaa tccgcataaa aaatacggtc caacccctgc ggcaaaattc
     9421 cgaccaacaa cttgcccagt tccaccgcag gatcgtgcgt caaaccgcca aacattacat
     9481 gcgacatttg cttaatctgc gcctcaaccg cctgattcaa aacaggatga ttgtaaccat
     9541 gtatcgcaca ccaccaagac gacatcccgt caatcagccg tgtgccgtcc gccaattcga
     9601 taaacacccc ttctgcacgt ttgacaggat aaacgggaag cggatcggtc atggaagtat
     9661 agggatgaag cagatgggta cggtcgaaat caagcaatga tgatgtgtgt tgatgttcag
     9721 acggcataag tttctctctt ttcttcttac tgtattcaaa cgcaaacgcg tattctactc
     9781 cgacagaccg cttcccacac ctttccatcc gtttcgggcg caaaaccgcg aaacaaatcg
     9841 ttccgcaaca taagcgcaca ccgtttcgca ttcctcaagc ccgattggaa tcagacggcc
     9901 caacgcccaa taccgtttca cgcagcctcc gcccaacgct tcaatcagtc atagatatag
     9961 tggattaaca aaaatcagga caaggagacg aagccgcaga cagtacaaat agtacggcaa
    10021 ggcgaggtaa cgccgtactg gtttaaattt aatccactat aaaacggcaa tccatacgat
    10081 acagatcata gcaacagcca tcgcaacagc gttagcaaaa tcaggggact ccgacatagg
    10141 cgcatagcac ctaccgatgc acggctcctc attcggctct atgaatacca tacccatcac
    10201 aaaatccacc gccaaaacca ggcacggctt cttatactta tgatagattt ccaccatcct
    10261 gctccatata tcccaaacat tcataccgta tatcccgcag gcaacaaatt ccgattgaag
    10321 gttacagccc t
//
LOCUS       DAWFJR010000067        10249 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWFJR010000067 DAWFJR010000000
VERSION     DAWFJR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10249
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.067"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..297)
                     /locus_tag="WGR04_001517"
     CDS             complement(<1..297)
                     /locus_tag="WGR04_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0795044.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRH"
     gene            536..1435
                     /gene="crgA"
                     /locus_tag="WGR04_001518"
     CDS             536..1435
                     /gene="crgA"
                     /locus_tag="WGR04_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ0795045.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSNKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1662..4877)
                     /gene="carB"
                     /locus_tag="WGR04_001519"
     CDS             complement(1662..4877)
                     /gene="carB"
                     /locus_tag="WGR04_001519"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ0795046.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQVLGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4906..5547)
                     /locus_tag="WGR04_001520"
     CDS             complement(4906..5547)
                     /locus_tag="WGR04_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795047.1"
                     /translation="MSIPINFNNLKYLLNDMRNKNRIIEAFPFNYNQRQYAVILTRYK
                     PDEPRPDDYAQAKLEFFNLNSENSIFAYADFYEVHFKSATDFINFFKINVQAGAAKIR
                     EIFQSFSNLFADFIPTQTKKDLDIIYKKIVATRLEPNSPNTIYCYDVRRNGKDKAGKP
                     NRRSVENSEKAKILRPELYEKFKADSNYSFFFSDNPSDEKTDAEIIREVTNRQ"
     gene            complement(6226..6615)
                     /locus_tag="WGR04_001521"
     CDS             complement(6226..6615)
                     /locus_tag="WGR04_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ0795048.1"
                     /translation="MSTHEKLLTADNPVLRQRAKAMRQEMSEAEAKLWQHLRASRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHAEQAVYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLAEILRVLQELEKQYAQ"
     gene            complement(7058..7435)
                     /locus_tag="WGR04_001522"
     CDS             complement(7058..7435)
                     /locus_tag="WGR04_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEZ0795049.1"
                     /translation="MKISALDHLILTVADIDRTIAFYTQVLGMEEVSFGNNRKALLFG
                     SQKINLHARGAEIQPNAQHAACGTADLCLLTDTPLETVLQELSAHGIKPLSGIAARTG
                     AMGKIQSVYLRDPDGNLLEISSY"
     gene            complement(7489..8622)
                     /gene="carA"
                     /locus_tag="WGR04_001523"
     CDS             complement(7489..8622)
                     /gene="carA"
                     /locus_tag="WGR04_001523"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ0795050.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
     gene            complement(8808..9131)
                     /locus_tag="WGR04_001524"
     CDS             complement(8808..9131)
                     /locus_tag="WGR04_001524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795051.1"
                     /translation="MPTGLDSRLRGNDGSGIPDDSGIPDDSGIPDDSGIPDDSGIPDD
                     SGIPDDSGIPDDSGIPDDSGIPIVSPDGRGEMRNGHSGRGAGGFTPRISVFARTYQPA
                     CRVCA"
BASE COUNT         2288 a         2639 c         2694 g         2628 t
ORIGIN      
        1 gtgtctgccg tcgctttggt agagtttgcc gtgtccagcg gttaatactt cgtctatgta
       61 ttttttcact gtccaaggct cgtgcatcca ccagcccggc atctgccaaa ttacagcatc
      121 catccaaacg aatttttcga tttctgcctc aacatcatag ccggcatcaa tcacggtttc
      181 ttgaacattg tgtccgagcg cggtcaaaac ttctttcgct tttttgtgaa gcgtgcggtt
      241 taacccgccg tgagaatgtc cgaacgcctt gccgccgtct aataataaaa tattcatttg
      301 ttacctcgtt tgtgaattga tgaatgtatt gtgccgtttt actttataat ttaaaagtgc
      361 aaaaataaga aaacactttt gcgtcaaatg aaataatcag atgttccaac acacgggatg
      421 gcacataaag cgtctcccta tgtgccgtcc tgattcggaa ggggttacac ccctcacaaa
      481 taaagtctga tcctgccgcc ctaaagggcg gggttccaac cgaaaaggaa acacgatgaa
      541 aaccaattca gaagaactga ccgtatttgt tcaagtggtg gaaagcggca gcttcagccg
      601 tgcggcggag cagttggcga tggcaaattc tgccgtaagc cgcatcgtca aacggctgga
      661 ggaaaagttg ggtgtgaacc tgcttaaccg caccacgcgg caactcagtc tgacggaaga
      721 aggcgcgcaa tatttccgcc gcgcgcagag aatcctgcaa gaaatggcag cggcggaaac
      781 cgaaatgctg gcagtgcacg aaataccgca aggcgtgttg cgcgtggatt ccgcgatgcc
      841 gatggtgctg catctgctgg cgccgctggc aacaaaattc aacgaacgct atccgcatat
      901 ccgactttcg ctcgtttctt ccgaaggcta tatcaatctg attgaacgca aagtcgatat
      961 tgccttacgg gccggagaat tggacgattc cgggctgcgt gcacgccatc tgtttgacag
     1021 ccgcttccgc gtaatcgcca gtcctgaata cctggcaaaa cacggcacgc cgcaatctac
     1081 agaagagctt gccggtcacc aatgtttagg cttcaccgaa cccggttctc taaatacatg
     1141 ggcggtttta gatgcgcagg gaaatcccta taagatttca ccgcacttta ccgccagcag
     1201 cggtgaaatc ttacgctcgt tgtgcctttc aggttgcggt attgcttgct tatcagattt
     1261 tttggttgac aacgacatcg ctgaaggaaa gttaattccc ctgctcgccg aacaaacctc
     1321 caataaaacg caccccttta atgctgttta ttacagcgat aaagccgtca acctccgctt
     1381 gcgcgtattt ttggattttt tagtggagga actgggaaac aatctctgtg gataattcta
     1441 taaaaacctg ccataaaatt gtttttactc acttaataac tttgagaaaa aataaataag
     1501 atgatgtata gccggcggca atggcagtct atatggggag cgtgataggg ctgttcctta
     1561 tgtacggctt gtgtgtggca acatcggctt tccaaatgac aaaatgccgt ctgacaccgt
     1621 aaaaaaaggt ttcagacggc attttcaacc tgattcaggc atcagttgcg gtttttcaaa
     1681 cgcccgtgca actcttgaac gctgtacacg cccagatggt ctcggctttt cgcgccttcg
     1741 ctcatcgctt cgccgccggc ggtggtggtg tattgcggca cgcgttgctg caatgcgctt
     1801 tggcggatga tgtggctgtc ggacacggat tgcggatcgc tggaaacggt gttcacgacc
     1861 agtgcgattt cgccgttttt cagcgcatcg ccgatgtgcg ggcggccttc ggggactttg
     1921 ttgatggtct gcacaatcag cccgtgttcg gtcaggtatt gcgccgtgcc gcgcgtggcg
     1981 cagatgccgt agcctaaaac ttggaagttt ttagcggttt taatgacgcg ttctttgtct
     2041 tcttcgcgca cggagaggaa gattttgccg gtcgggttga ggcgttcgcc tgcgccgagt
     2101 tgggctttgt agtaggcttc gccgaagctt gcgcccacgc ccatgacttc gccggtggag
     2161 cgcatttccg gtcccaaaat cgtgtccacg cccgggaatt tgatgaatgg gaacacggct
     2221 tctttaacgg catagaaatc ggggacaact tctttttcca cgccttgttc tttcagggaa
     2281 atacctgcca tgcagcgtgc gccgactttg gcgagcggca cgccggtggc tttggagacg
     2341 aagggcacgg tacggctggc gcgcgggttc acttccaaca cgaacaccac gccgtcctgc
     2401 acggcaaact gcacgttcat cagaccgacc acgcccagcg cgtacgccat cgctttggtt
     2461 tggcggcgga tttcgtcttg gatttcttcg cttaaggagt agggcggcag cgagcagcca
     2521 gagtcgccgg agtgaatgcc tgcctgttcg acgtgctgca taatgccgcc gataaccacg
     2581 tctttgccgt ctgaaacgca gtccacatcc acttcaatcg cgttgttgag gaagaaatcg
     2641 agcaacacgg ggctgtcttc ggaaacctgc acggcttcgc gcatgtattt ttgcaaggct
     2701 tcggcagagt ggacaatctg catcgcgcgt ccgcccaaaa cataagacgg gcgcacgacc
     2761 agcggatagc cgatttcttc ggctttgacg agtgcttctt cttcgttgtg ggcgatgcgg
     2821 ttgggcggtt ggcgcagacc taagtcgttc aacacttttt ggaagcgttc gcggtcttcg
     2881 gcggcgtcga tgctgtcggc ggacgtgccg atgatgttta cgccgttttc aaccaacgcg
     2941 ttggcgagtt tcagcggggt ttgaccgccg taatgcacaa tcacgcccca cgggttttcg
     3001 gtgcggacga tttccaacac gtcttccaat gtcagcggct cgaaatagag gcggtcgctg
     3061 gtgtcgaagt cggtggacac ggtttctggg ttgcagttga ccatgatggt ttcaaagccc
     3121 gattcgcgca gggcgagtgc ggcgtgaacg cagcagtagt caaactcgat gccctgaccg
     3181 atgcggttcg ggccgccgcc gagaatcatc acttttttac ggtcggaagg acgggattcg
     3241 cattcctctt cgtaagtgga gtagaggtag gcggtttcgg tggcaaactc ggcggcacag
     3301 gtgtcgacgc gtttgtaaac cggatgcagc ttcagcgcgt agcggtgttc gcgaacttct
     3361 ttttcgctta cgttcaacaa ttgtgccaaa cgtttgtcgg agaagccttt gcgtttcaga
     3421 cggcgtaggg cggcgaaatc caaatcactc aaaatgccgt ctgaaaccgc tttttcttcc
     3481 ttcatcaagt cttcgatttg cgccaggaac caagggtcga tggcgcagat ttcgtggatt
     3541 tcttccagcg tgaagcccgc gcggaacgcg tctgccacaa acagcatacg ttcggggccg
     3601 gggtttgcca gttcgcggcg gatttccgct ttgtcggagc ttctcggatt gaagccgcac
     3661 aagcctgttt ccaagccgcg cagggctttt tggaagcttt cctgaatcgt gcggcccatc
     3721 gccatcactt cgcccacgga tttcatctgc gtggtcaggc ggtcgtctgc ggcagggaat
     3781 ttttcaaatg cgaaacgcgg gattttggtt accacatagt cgatggaagg ctcgaacgac
     3841 gcgggggttt tgccgccggt gatgtcgttg cgcaactcgt ccagcgtaaa gccgaccgcc
     3901 agttttgccg ccaccttcgc aatcgggaag cccgtcgctt tggaagccag cgcggacgaa
     3961 cggctcacgc gcgggttcat ctcaatcaca atcatctcgc cgttttcagg gttcaccgca
     4021 aactgaacgt tcgagccgcc cgtgtccacg ccgatttcgc gcaataccgc cagcgaagcg
     4081 ttgcgcatga tttggtattc tttgtccgtc aacgtttgcg ccggcgcaac cgtaatcgag
     4141 tcgcccgtat gcacgcccat cgggtcgaag ttttcaatcg agcagatgat gatgcagttg
     4201 tcgtttttat cgcgtacgac ttccatctcg tactctttcc agccaaggac ggactgctca
     4261 atcagcagct catgcgtggg cgaggcatcg aaaccgcgtt cgcaaatcgc caaaaactcg
     4321 tctttattgt aggcaatacc gccgcccgaa ccgcccatcg tgaaagaagg acgaatcagc
     4381 gtcgggaagc cgacctgctc ctgcgccgcc aaagcttcgt tcatcgtgtg gcagacaaaa
     4441 gatttcgggc aagacaaacc gattttttcc atcgcttctt taaagcggcc gcggtcttcc
     4501 gccttgtcga tcgcgtcttc cgtcgcgcca atcagctcga cattgtattt tgccagcacg
     4561 ccgttgcgtg ccaaatccag cgcacagttc agcgcggtct gaccgcccat cgtgggcaga
     4621 atcgcatcag gccgctcctt ggcgataatc ttctccaccg tctgccacat aatcggctcg
     4681 atgtaggtaa catccgccat ttcagggtcg gtcataatcg tggcggggtt ggaattcacc
     4741 aaaatgactt tatagccttc ttcacgcaag gctttgcagg cctgtgcgcc cgaatagtca
     4801 aattcgcagg cctgaccgat aacgataggg ccggcgccga tgataaggat ggattttagg
     4861 tcggtacgtt tgggcatggt gggtctttct tttgaagaat ttggattatt gacgattggt
     4921 aacttctcta attatttctg catctgtttt ttcatcgctt ggattatctg aaaagaaaaa
     4981 actgtaatta ctatcggctt taaatttttc gtatagctct gggcgcaaaa tttttgcttt
     5041 ttcactattt tccacgctcc tgcgattagg cttgccagcc ttatctttcc catttctacg
     5101 gacatcatag caataaatag tgttaggaga attaggttct aaacgagtag ctacaatctt
     5161 tttataaatt atgtctaaat cttttttagt ttgtgttgga atgaaatctg caaaaagatt
     5221 actaaaactc tgaaaaattt ctctgatttt cgcagcacca gcctgaacat taattttaaa
     5281 aaaattaata aaatcagtag cacttttaaa atgaacttca taaaaatcag catacgcaaa
     5341 tattgaattt tcactattca aattaaaaaa ctctaatttt gcttgtgcat aatcatctgg
     5401 tctaggttca tcaggtttat acctagtcaa aataacggcg tattgccttt gattataatt
     5461 aaaaggaaat gcttcaatta ttctattttt gtttctcata tcattaagca aatactttaa
     5521 attattgaaa ttaataggta ttgacatatt taaactcctg cgaatatgtt gcaataaacg
     5581 tagcaatcgt aggttgggtt gaaaacccaa cacaaaccgc ttaattgtct aaaatgttgg
     5641 gtttgctaac ctaacttgta tatcgaaacc cccgtattgt agcaagccgt agcccgtatg
     5701 tagggtatgt gcggtacgta tgcacgcgtt ctccattttc cctgcaaccc caacccaccg
     5761 cgtgcgtgcc ttgcggcaca ctctctgccg atgggtttaa aggtcgtctg aaaaaacgct
     5821 cgcggcgttt gttccatctg ccttgcgata aaggctgcct gaaaaattcc cgctgttgat
     5881 atttcgggca ttctttctca aaccgcctgc aaccgcaaaa tccgctgcaa tctgccccct
     5941 cccccgtggg ggaggaccgg ggagagggca ttcttcgaat ttcggcaacc ttccccaaca
     6001 ccctaaccgc tcaaacacaa gccttgcggc ttgttgccct ctctctggct ctctcccacg
     6061 ggagagagga cgtggtggct gttggaatta agggttctgc aggaaaaaca ggttgtgcgg
     6121 gctgtttttt gtttttcaga cggtcttcta aacgctcaat tcaagcagcc tgtaccctac
     6181 ctgtatccta taattttcaa aactctaatc aaaattaacc gtttgttatt gcgcatactg
     6241 cttttccaat tcctgcaata cgcgcaggat ttccgccagt acatcgtttg tctgctgcaa
     6301 aatttcgtga ttccaaaaac gcagcacggt aaagcccagg ctgttgagat atgccgtccg
     6361 cgcgtggtcg tatacggctt gttccgcgtg ctgcccgccg tctgcttcga caatcagctt
     6421 gggcgttacg cacataaaat caacaatata gtttcccatc ggctgctggc ggcggaattt
     6481 gtagccattt agacggcttg cccgcaggtg ttgccacaat tttgcttctg cctcgctcat
     6541 ttcttggcgc atggctttgg cgcgttggcg caggacgggg ttgtcggcgg tcaagagttt
     6601 ctcgtgcgta ctcattttgt tgtcgggttg ttgtttgtac aagcctttcg gcttactgcc
     6661 ctctctctag ctctctccca cagggagaga ggacggggcg gcggttgggg ttaagggttc
     6721 tgtaaactaa acagcttgtg caggctgttt tttacttttc agacgacctg aaatcaacgg
     6781 ctgtaaatcc aagttgcttt tcaaatcgcc tgcaaccgtg aaacttgccg caaccggctc
     6841 cctcccccgt gggggagggt tggggagagg gcattcctcg aatttcggca atcttcccaa
     6901 ataccctaac cgccccaata caagcctttc ggcttactgc cctctctcta gctctctccc
     6961 acagggagag aggatggggc ggctgttggg gttaaagagg ctgcaaggaa aacagcttgt
     7021 gcaggctgct ttttattttg cataagccgt ctgaaaatca ataactgctg atttccagca
     7081 ggttgccatc gggatcgcgc aggtaaaccg attggatttt gcccattgcg cctgtgcgcg
     7141 ctgcgatgcc gcttaaaggt ttgatgccgt gtgcggataa ttcctgtaaa accgtttcca
     7201 gtggcgtatc ggtcagcagg cataaatccg ctgtgccgca ggcggcgtgt tgcgcgttag
     7261 gctgaatttc cgcaccgcgc gcgtgtaggt tgattttctg actgccaaac aacaaagcct
     7321 tacggttgtt gccaaatgaa acttcttcca tacccaaaac ttgcgtgtaa aacgcaatgg
     7381 ttcggtcaat gtcggcaacg gtcagtatca gatggtcgag tgcgctaatc ttcatttcca
     7441 gctttgtttt tcagacggca gcagcatact gttgccgtct gaaagccatt atgcccgttt
     7501 tgccgctttc atattgccaa tgaatttgtc aaacaaatag ccgacatctt gcggaccggg
     7561 gctggcttcg gggtggcctt ggaagcagaa cacaggtttg tcggtcagct cgatgccttg
     7621 caaagtgttg tcaaacaagg atttgtgggt aatgcgtgcg ttagcgggca gggtgtcggc
     7681 atcaacggca aaaccgtggt tttggctggt aatgacgact ttgccgctgt ccaaatcttg
     7741 cacagggtgg ttcgcaccgt ggtggctgaa gcgcattttc agggttttcg cgccgatagc
     7801 gaggctgatg agctggtgtc ccaagcagat gccgaaaatc ggtttgccgc tttccatcag
     7861 tttttgcacg gcttcgatgg cgtaggtgca aggctcgggg tcgccggggc cgttggacag
     7921 gaacacgccg tcgggattga gtgccaacac gtcttgtgcg ctcgtttggg cggggacgac
     7981 ggtcaggcgg cagccgcgcg aggcgagcat acgcaggatg ttggttttca cgccgaaatc
     8041 gtaggcgacg acgtggtaag gctgttcgtc aggggtaacg aaacccttgc ccaattccca
     8101 ttcgccttcc gtccattcgt aagtttccgt gcaggaaact tcttttgcca agtctttgcc
     8161 gaccatactg ccgaacgcgg cgatgagttc ttgcgctttt tcgattgtgg catccgcacc
     8221 ggtcagaatc gcgccgcctt gcgcgccttt ttcgcgcaac agcgtggtca ggcggcgggt
     8281 atcgatgtcg gcgatggcga cggttttgtt gcgtaccaaa tagtcgtgca ggctttcgga
     8341 ggcgcggaag ttgctgtgca agagcggcag gtcgcggata atcaggccgg cggcgtaaac
     8401 gctgcggctt tcttcatctt cggcgttggt gccggtgttg ccgatgtggg ggtaggtgag
     8461 ggtaacgatt tgtttgcagt aggacgggtc ggtcaggatt tcctgatagc cggtcatcga
     8521 agtattgaac acgacttcgc cggaagtcga accttcgtaa ccgattgatg tgccgtggaa
     8581 tacgctgccg tcagcgagga cgaggagggc gggggtgctc atgatgggaa tcctgttttt
     8641 aataaaattc ttgacaatgc cgtctgaagg gacttcggca gggggcatcc ggtcggaaaa
     8701 atccggtcaa aaaaaaacac gccgcgcaag atagacgcgt aacgtgcttt tcgggcaagt
     8761 gcctataccc tgaaagcagc atattttaag acgaaacata cccgatttca agcgcaaacg
     8821 cggcaggcgg gctgatatgt tcgcgcgaaa acggaaatcc gtggcgtaaa accgcccgct
     8881 ccgcgcccac tatgcccgtt acgcatttcc ccacgtccgt ccggcgaaac tatgggaata
     8941 cccgaatcgt caggaatacc tgaatcgtca ggaatacctg aatcgtcagg aatacctgaa
     9001 tcgtcaggaa tacctgaatc gtcaggaata cccgaatcgt caggaatacc cgaatcgtca
     9061 ggaatacccg aatcgtcagg aatacccgaa ccatcattcc cgcgcaggcg ggaatccaga
     9121 cctgtcggca cagaaactta tcgggtataa cggtttcttg agattccgca ttctagattc
     9181 ccgcctgcgc gggaatgacg gcgcataagt ttctgtgttg atttgagtct atgggaacgg
     9241 tgtaggattg gtggaatcgt tggaatcggt gggctgaagc ccaccctata actcaccttt
     9301 tacgaatcct cctacaccct atacaacacc ttgaaaatcc accttgtcgg gaatgcccga
     9361 accgtcagaa atacccgaat cgtcattccc gcgcaggcgg gaatccagac ctgtcggtgc
     9421 agaaacttat cgggcaaaac ggtttcttga gattccgcat cctagattcc cgcctgcgcg
     9481 ggaatgacgg ctgcagatgc ccaacggtct ttattcctcc atctctaaga tttacagcga
     9541 tacacgggta gtttaaggaa tgtccgaatc gtcattccca cgaaagtggg aatccagttt
     9601 tttgagtttc agtcatttcc gataaattgc cttagcattg aatgtctaga ttcccgcctg
     9661 cgcgggaatg acggcatcgg tttgtcggcg tttcatttgc ccccatcccg aaaacgcaaa
     9721 caataccgtc tgaaagctct gtagggaggg cttcagccca ccgattccat caatcccaca
     9781 tttccgccgt tttcaccgaa tccgcccctt ttcacaatcc agtcctacgc aactgaaccg
     9841 tcattcccgc gcaggcggga atccggactt gtcggcacgg aaacttatcg ggtacaacgg
     9901 tttcttgaga ttttacgtcc tagattcccg cctgcgcggg aatgacgcgt ttcatttgcc
     9961 gccccctccc gaaaaacgca aaaaaatgcc gtctgaagac ctttcagacg gcaatcgcaa
    10021 cagccgcacc gtcctctctc ccgcgggaga gagttagaga gggggcagca agccgcaagg
    10081 cttgtatttt agaaggctaa gggatttggg aaaggttgcc gcaacttgga aaatgcccct
    10141 gcccggccct cccccgcagg agaggggggc ggattgcggc agatttggcg gttgcaggcg
    10201 gtttgattgc agggcaaccg gccgcgcagg cgggtcagta gaagacttc
//
LOCUS       DAWFJR010000068        10085 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWFJR010000068 DAWFJR010000000
VERSION     DAWFJR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10085
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.068"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..111
                     /locus_tag="WGR04_001525"
     CDS             <1..111
                     /locus_tag="WGR04_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0795052.1"
                     /translation="RAAYFGLIKVSAQSHLKAMCLNLLKAANRLSTPAAA"
     gene            903..1721
                     /gene="opc"
                     /locus_tag="WGR04_001526"
                     /gene_synonym="opcA"
     CDS             903..1721
                     /gene="opc"
                     /locus_tag="WGR04_001526"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ0795053.1"
                     /translation="MKKTVFTYAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWSAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTSLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
     gene            1885..2529
                     /locus_tag="WGR04_001527"
     CDS             1885..2529
                     /locus_tag="WGR04_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0795054.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            2548..4455
                     /locus_tag="WGR04_001528"
     CDS             2548..4455
                     /locus_tag="WGR04_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0795055.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGLQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGKIRDLLRRYH
                     RVGHELENGSGEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELNPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFHPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     VFEGQGRLKEYIGGYQDYIDAKSREDKIQTASAPKAVAEPEKVKPKANRTVKLSYKEQ
                     RELDALPDEIAALETEQAEINTRLSDPEIFKDYEKAGALQNRAEEIEMLLLEKLERWE
                     LLETKRNGNAV"
     gene            4476..4841
                     /locus_tag="WGR04_001529"
     CDS             4476..4841
                     /locus_tag="WGR04_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795056.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            4952..6427
                     /locus_tag="WGR04_001530"
     CDS             4952..6427
                     /locus_tag="WGR04_001530"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ0795057.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIRAAQMVQEGLDK"
     gene            6424..6969
                     /locus_tag="WGR04_001531"
     CDS             6424..6969
                     /locus_tag="WGR04_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ0795058.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRSAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            6966..8102
                     /locus_tag="WGR04_001532"
     CDS             6966..8102
                     /locus_tag="WGR04_001532"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ0795059.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDA
                     PAAEFADCHLCAPFNDQAALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQVVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHGGVDCVLEKMVDLRSEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQMAQRLADELDYVGVLAVEMFVVDDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            8149..8631
                     /locus_tag="WGR04_001533"
     CDS             8149..8631
                     /locus_tag="WGR04_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0795060.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            8737..9792
                     /locus_tag="WGR04_001534"
     CDS             8737..9792
                     /locus_tag="WGR04_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0795061.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKATNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEKKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            9789..10067
                     /locus_tag="WGR04_001535"
     CDS             9789..10067
                     /locus_tag="WGR04_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795062.1"
                     /translation="MTAMPSEPPFRRHFTHQNRKTAPKIIITKHNGKPDTKPNTAAKM
                     PSEAVGATILIWGITFLNHPFDSQINSAFACQKQNQYFAVRIGTFIVD"
BASE COUNT         2794 a         2818 c         2250 g         2223 t
ORIGIN      
        1 cgggcagcct atttcggact gattaaagtg agtgcgcaaa gccacctgaa ggcgatgtgt
       61 ttgaacctgt tgaaagccgc caacaggcta agtacgcccg ctgccgccta aaaggcggcc
      121 cggatgcctg attatcgggt atccggtgag gcttaagggg gtatttgggt aaaattagga
      181 ggtatttggg gcggaaatag acgaaaacct gtgtttgggt ttcggctgtc gggagggaaa
      241 ggaattttgc aaaggtctca aacaaaaaca gaaacctaaa gtcccgtcat tcccgcgcag
      301 gcgggaatcc agacccccaa cgcggcagga atctatcgga aataaccgaa accggacgaa
      361 cctagattcc cgctttcgcg ggaatgacgg cagggtggtt tcagttgctc ccgataaatg
      421 ccgccatctc aagtctcgtc attcccttaa aacagaaaac cgaaatcaga aacctaaaat
      481 cccgtcattc ccgcaaaagc gggaatctag gtttgtcggc acggaaactt atcgggtaaa
      541 acggtttctt tagattttac gttctagatt cccgcctgcg cgggaatgac gatgaaaaga
      601 ttgttgtcgc ttcggataaa tttttgccgt gttgggttct agattcccgc tttcacggga
      661 atgacggcgg cgggtttctg ttttccgata aatacacaca aactaaaatt tcgtcattcc
      721 cataaaaaac agaaaaccaa gtgagaataa caattcgttg taaacaaata actatttgtt
      781 aatttttatt aatatatgta aaatcccccc cccgaaagct taagaatata attgtaagcg
      841 taacgattat ttacgttatg ttaccatatc cgactacaat tcaaatttta gaggttttaa
      901 ctatgaaaaa aacagttttt acatatgcca tgatcgccct gaccggtact gccgccgctg
      961 cacaagagct tcaaaccgct aatgagttta ccgtccacac cgacctctct tccatttctt
     1021 caactcgtgc tttcctgaaa gaaaaacaca aagctgccaa acacatcggc gtgcgtgctg
     1081 atattccttt tgatgccaac caaggcatcc gcttggaagc cggtttcggg cgcagcaaaa
     1141 aaaatattat taatttggaa acagatgaga acaagctggg taagactaaa aatgtaaaac
     1201 tgcccaccgg cgttcctgaa aaccgtatcg atctttacac aggctacacc tacacccaaa
     1261 cgttaagtga ttctttaaat ttccgtgtgg gtgccggctt gggttttgaa tcttcaaaag
     1321 acagcattaa aaccaccaag catacgcttc acagcagccg tcagtcgtgg tcagccaaag
     1381 ttcacgcgga tttgctttcc caactgggta acggctggta tatcaaccct tggtctgaag
     1441 tgaaatttga cctcaattcc cgctataaat taaacaccgg cgttaccagt ctcaaaaaag
     1501 acatcaatca aaaaaccaac ggctggggct ttggattggg tgcaaatatt ggtaaaaaac
     1561 tgggcgaatc cgccagcatc gaggcggggc cgttctacaa acaacgcact tacaaagaat
     1621 ccggcgagtt tagtgtaaca accaagagtg gcgacgtatc gctcaccatc ccgaaaacca
     1681 gtattcgtga atacggcttg cgcgtcggca taaaattctg atgatttgaa atcatcattg
     1741 tcactttaaa tgccaaaccg caaactattt tggtttgcgg tttttacgtg aaatgaattt
     1801 gaatagccga tgccgtctga aacttttcag acggcatttt tccaatcggt taaaatacgc
     1861 ggtttgtttt tttaggaacg cgccgtgctt gcctccgcca tcgacttcat cctccatatc
     1921 gaccaacacc tgctcgcgct gtcggcgcaa tacggtgtgt ggatttatgc gattctgttt
     1981 ttgattgttt tttgcgaaac cggcctgatt gttacgccct tactgcccgg cgattcgctg
     2041 ctgtttgccg ccggcgggat tgctgcgctg ggcggtatgg atattcattt gatggttgca
     2101 ttattaagcc ttgccgccat actcggcgac gcgctcaatt ttaccgtcgg caaatatttc
     2161 ggcggcaggc tgttcgccaa ccccgattct aaaatcttcc gacgtgaata tctcgataaa
     2221 acccaccgtt tttacgaaaa acacggcggc aaaaccatca tcattgcccg ttttatgccc
     2281 atcgtccgca ccttcgcccc cttcgtcgcc ggtatgggga aaatgcacta tgccaaattt
     2341 atccgctaca acatcatcgg tggcttgttg tgggttatcc tgttttctta cgcgggctat
     2401 ttctttgcca acttccccgt cgtaaaaaac aatctgggct tggtgatggg cggcatcatt
     2461 attgtttccg tcctgccggg tgcggttgaa attgcccgcg ccaaacttgc ggtaaaatcc
     2521 aaacggtaac tgttgagaat ttgcatcatg aatatattat ctgtagaaaa cgcttctttt
     2581 gccatcggtc acgttgcctt gctcgacaaa acttcttttc aactcgacag cggcgaaaaa
     2641 gtcggcttaa tcggacgcaa cggcgcaggc aagtcttcgt ttttaaaaat cctcgccggt
     2701 ttgcaaaagc tcgacgacgg gcagattatt gttcaaaaca acctcaaaat cgtttatgta
     2761 ccacaggaat ccttttttga taaggacgca accgtatttg ataccgttgc cgaaggtttg
     2821 ggtaaaattc gcgatttatt gcgccgttac caccgcgtcg gtcatgagtt ggaaaacggt
     2881 tcgggtgagg ctttgttgaa agaactcaac gaattacaac ttgaaatcga agcgaaggat
     2941 ggctggaagc tggatgcggc agtcaagcag actttgggcg aactcggttt gccggaaaac
     3001 gaaaaaatcg gcaacctttc cggcggtcag aaaaagcgtg tcgccttggc gcaggcttgg
     3061 gtgcagaagc ccgacgtatt gctgctggac gaaccgacca accatttgga tattgacgcg
     3121 attatctggt tggaaaacct gctcaaggcg tttgaaggca gcttggttgt gattacccac
     3181 gaccgccgtt ttttggacaa tatcgccacg cgcatcgtcg aactcgaccg aggtattttg
     3241 cgttcctatc ccggctcgtt ttccaaatac agcgagaaaa aagcgcaaga gttggcggtc
     3301 gaggcggaac ataaccgcct cttcgacaaa ttccacgcgc aggaagaagc gtggatacgc
     3361 aaaggcatcg aagcgcgccg tacccgcaac gaaggccgcg tgcgccgttt ggaagagctg
     3421 cgccgccaac gcgccgaacg ccgcaatgtg caaggtcagg tcaacttcaa gctcgacagc
     3481 ggcgagaaaa gtggcaaaat catcgccgaa ttggaacacg cttcgtttgc ctatggcgac
     3541 aaagtcatta tggacaaatt ctccgcgatt ttgcagcgcg gcgacaaaat cggcctaatc
     3601 ggccccaacg gtatcggcaa aaccacattt ttaaaactga ttttgggcga actcaacccg
     3661 acttacggca gaatccgtat cggcagcaag caggaagttg cctatttcga ccaattccgc
     3721 agcgcgttga acgaaaacga caccgtgttt tacacgctcg gacagggcaa tgattatgtc
     3781 gaagtcggcg gcaagaaaaa acacgtaatg agctatctgg aagatttcct gttccaccct
     3841 gcccgcgcgc aaagccccgt ttcatcactc tccggcggcg aacgcaaccg ccttttgctg
     3901 gcgaaactct ttacccgtcc cgccaatatt ttggttttgg acgaaccaac caacgacttg
     3961 gacatcgaca cccaagagtt gctcgaagac ctgttgcgcg actaccaagg cacggtattc
     4021 ctcgtctcgc acgaccgtat gttcttggat aatgtgatta cccaaagcat tgttttcgaa
     4081 ggacaaggtc gtctgaaaga atacattggc ggctatcagg actatatcga cgcaaaatca
     4141 cgggaagata aaattcagac agcctctgcg cctaaagcgg ttgccgagcc tgaaaaagtc
     4201 aaacccaaag ccaaccgcac ggtcaaactt tcctacaaag aacagcgcga actcgacgcc
     4261 cttcctgacg aaatcgcagc tttggaaacc gagcaggctg aaatcaatac ccggctttcc
     4321 gatcctgaaa ttttcaaaga ttacgaaaaa gcaggtgcgt tgcaaaaccg cgccgaagaa
     4381 atcgaaatgc tgcttttgga aaagctggaa cgctgggagc tgctggaaac caaacggaac
     4441 ggcaatgccg tctgaaatca aacaggaaga aaaaaatgtt caaacgacct gaagaaatca
     4501 tcgtcctgat acttgccgtc ctctggattg ccggtacgta ttttctcgcc gccctgtttg
     4561 gagcggacgc ttataccgtg ttaaaaatca ctgcccttac cctgctttgg tctgccgcat
     4621 cattcctcct ttggcaaaaa aacctcagcc ggcatatctg gccgctgctg ctcggctgcc
     4681 tgactgcctg ttggtggccg tatctgaatc aatcggtcgc acccggttct ttaccttggt
     4741 atgcatcatg gacgttcaaa accatcttag ccctgattcc cgtaaccgca ggttatctgt
     4801 ttaaatggaa acaataccgg cagcgcaaaa caaagccgta agcccgtctt tttcagacgg
     4861 catctccggt cagactgtca acaattccga taaaatgcta tactcgcgcc cgttgccgcg
     4921 ccgtccgaaa ccgccctttg ggaaagcaga aatgatcagc aaacaagaat accaagccca
     4981 agccgcccaa ggctacaacc gcatcccgct cgtgcaagaa ctccttgccg atttggatac
     5041 gccgctttcc ctctatctca aactcgccaa ccgcccctat acctacctgc tcgaatccgt
     5101 tgtcggtggc gaacgtttcg gccgctattc ttttatcggc ctgccttgca gccactatct
     5161 caaagccagc ggcaaacacg tcgatgttta tcaaaacggc gaaattgtcg agcaacacga
     5221 cggcaacccc ctgcccttta tcgaagcctt ccacaaccgc ttcaaaacac ccgaaatccc
     5281 aagcctgccg cgctttaccg gcggactggt cggctacttc ggttacgaaa ccatctacaa
     5341 ttttgaacac ttcgcccacc gcctgaaaaa caccaccaaa gccgacccgc tcggcacgcc
     5401 cgacatcttg ctgatgctgt cgcaagaatt ggcggtgatc gacaatttga gcggcaaaat
     5461 ccatctcgtc gtttacgccg atccgtcgca gcccgacggc tacgaacgcg cccgcgaacg
     5521 cctcgaagac atccgcaccc agttgcgcca aagctgcgcc atcccgctct cgctcggcag
     5581 caaacacacc gaagccgtca gcgaattcgg cgaagagccg ttcaaagcct gcgttaacaa
     5641 aatcaaagac tacatcttcg caggcgactg catgcaggtt gtccccagcc agcgcatgag
     5701 catggaattc accgacagcc cgctcgccct ctaccgcgcc ctgcgtacgc tcaacccttc
     5761 gccttatctc ttttactacg atttcggcga tttccacatc gtcggctcct cgcccgaaat
     5821 cctcgtccgc cgcgaacgcg acgatgtcat cgtccgcccc atcgccggca cgcgcctgcg
     5881 cggcaaaacc cccgccgaag accttgccaa cgaacaagat ttgttaagcg atgccaaaga
     5941 aatcgccgaa cacgtcatgt tgattgactt aggacgcaac gacgtcggcc gcatcagtaa
     6001 aaccggcgaa gtcaaagtta ccgacaaaat ggtgattgaa aaatactccc atgtaatgca
     6061 catcgtttcc aacgtcgaag gccgtctgaa agacggcatg accaacatgg acatcctcgc
     6121 cgctaccttc cctgccggta cactctccgg cgcacccaaa gtccgcgcca tggaaatcat
     6181 cgaagaagtc gaaccgagca aacgcggcat ctacggcggt gccgtcggcg tatggggttt
     6241 caacaacgac atggatttgg caatcgccat ccgcaccgcc gtagtaaaaa acaacacgct
     6301 atacgtccaa agcggcgcgg gcgtggttgc cgactccgat ccggcctccg aatggcagga
     6361 aacacaaaac aaagcccgag ccgttatacg cgccgcgcag atggtgcagg aagggttgga
     6421 taaatgactt atgaaaaact ttatgagctt cgccaaacac tcagaccgac taccaaagac
     6481 ggtctctata cagataatga aaaaagtcga gaaatcctaa cggttagatg gagcggcaac
     6541 acagaaaatc ctaaaaattt ctcagctgtt gctataggaa ttaatccaag taaagcaaat
     6601 gatgaaagaa gcgacaaaac ccttacccaa cttgcacgtt tcttggatat gtacggtttt
     6661 accaacttca aaatgttaaa tattttttcc agttactcta cacagcaaac aggaattagg
     6721 gcaaacaccc aaaccgattt ttccaaattt aaaggatgtt tggaagatgc tgatatgatt
     6781 attcttgcat ggggtacaga ccgcagtgct tataaggatg aaaaaaacag aatactggaa
     6841 tttctaaaag cagaaaaatt tatggaaaaa gtattctgta tttctgaaac tggtaattcc
     6901 agcgatacaa gacatccgtc aagaattagt tatagctatc aacttgtaca atttgaagag
     6961 tcagcatgaa aaacatatct ctttctccgc ccgccatgct cggcattctt ggcggcggac
     7021 aattaggcag aatgtttact gttgctgcca aaaccatggg ctacaaagtc accgttctcg
     7081 accccgatcc ggacgcgccg gcagcagaat ttgccgactg tcatttatgc gcgccgttta
     7141 acgaccaagc tgctttggac gaattggcaa aatgcgcggc ggtgaccact gaatttgaaa
     7201 acgtcaatgc cgatgcgatg cgttttctcg ccaaacacac caatgtttcc cccagcggcg
     7261 actgcgtggc gattgcacaa aaccgcattc aggaaaaggc atggatacgc aaagcaggat
     7321 tgcaaaccgc gccgtatcaa gtggtttgta aggctgaaga cattactgaa gcaagtgcgc
     7381 aattcttgcc cggcatcctg aaaacggcca ctttgggcta cgacggcaaa ggtcaaatcc
     7441 gcgtcaaaac ggtggatgaa ctcaaagctg cgtttgccga acacggcggc gtggattgcg
     7501 ttttggaaaa aatggtggat ttgcgcagtg aaatttccgt aatcgtatgc cgtttgaaca
     7561 atgacaacgt gcaaactttc gaccctgccg aaaacatcca cgaaaacggc atcttggctt
     7621 attccatcgt ccccgcgcga ctgagtgccg acgtgcagca acaggcgcgg cagatggcgc
     7681 aacgcttggc ggacgaattg gattatgtcg gcgtattggc ggtagaaatg tttgttgtcg
     7741 acgacacaca tgaattggtc gtcaacgaaa tcgcgccgcg tccgcacaac tccggccacc
     7801 ataccgtcga tgcctgcgcg gcagaccaat tccagcaaca ggtccgccta atgtgcaacc
     7861 tgccacctgc cgataccaag ttgctttctt catgctgtat ggcgaatatt ttgggcgacg
     7921 tttggcagga agacggcggc gaaccggatt ggtttcccct gcaaagccgg ccggacgcgc
     7981 acctgcacct ttacggcaaa aaaaccgcgc acaaagggcg gaaaatggga cactttaccg
     8041 ttttaaccac cgattcggac accgcatttc aagaagcaaa aaaactgcat cagtccctat
     8101 aatccgacca acggcacaga tgccgtctga agccgccgac atactgccat gaacctgctg
     8161 acactgttac gccccgccac cgtgcaagac tgcaaagaca tcttcaaagt ccacctgcac
     8221 tccgtccaat acacctgcat cctcagctac aacgaacacg ccctaaaagt atgggaaggc
     8281 ttgttaaaca cagaaagcta cctgccgacc atttccgatc ccgacaaagc actttgggtt
     8341 gccgaataca aaggcaacat acagggattt ttccaaatcg actgccaaga agcacaactc
     8401 gatgcacttt acgtccaccc cctgttccac aacctcggac tgggcactgc cctgcttcat
     8461 caggcggaaa ccatcgccca caaatccggt ttgagctttc tgaaactcta cgcatcgctc
     8521 aactccgtcc cgttttacct tctcaaccgc tacgaatcgc tcggttcggc agtattgcag
     8581 cttgacccca gcatcaaaat caaatgcgaa ctgatgcgga aacacctctg atccgtcctt
     8641 ataaccaaaa ctgataatct tcaaatcaaa cattatttcc acaagcgcag acagccgtat
     8701 aaagttgccc catctaccaa ccgacaggac aagcatatga aaacctacgc accggcactc
     8761 tataccgcag ccctgctcac cgcctgcagc cccgcagccg attcaaacca tccgtccgga
     8821 caaaatgccc cggccaatac cgaatccgac ggaaaaaaca ttaccctgct caacgcctca
     8881 tacgatgtgg cacgggattt ttacaaagaa tacaacccct tatttatcaa aacataccaa
     8941 tccgaacacc ccggcacatc cgtcagcatc caacagtccc acggcggctc cagcaaacag
     9001 gcattatccg tagccaacgg ccttcaagcc gatgtcgtaa ccatgaacca atcctccgac
     9061 atcgacctgc tcgaaaaaaa aggactggta gaaaaaggct ggcaacaagc cctccccgac
     9121 cacgccgcgc cctacaccag cactatggtt ttccttgtcc gaaaaaacaa ccccaaacag
     9181 atccgcgatt ggaacgacct tgccaaagac ggcgttaaca tcgtcatcgc caatcccaaa
     9241 acctcgggca acggacgcta cgccttcctc ggcgcatacg gttacggtct gaaagccacc
     9301 aacggcaacg aacaggaagc ccaaaaactc gtcgcatcca tcctcaaaaa cacccccgtt
     9361 tttgaaaacg gcggacgcgc cgccaccacc accttcacac aacgcaacat cggcgacgta
     9421 ctcatcactt ttgaaaacga agccaactac gtcagcaaaa aactgaccca aggacagttt
     9481 gaaatcgtct atccgagcta caccatttcc gccgaaagcc ccgtcgccgt cgtcaacagc
     9541 gtcgtcgcga aaaaaggcac gcaaaaaacc gcccgcgcct atctcgaata cctttggagc
     9601 gaaccggcgc aagaactcgc cgcctcactc tacctgcgcc cccgcaaccc cgaagtattg
     9661 gcaagacaca aagccgactt cccagactta gacaccttct ctcccgaaaa aaaattcggc
     9721 ggatgggaca acatcatgaa aacctacttc gccgacggcg gcatattcga ccgcctgacc
     9781 gcacaaaaat gaccgctatg ccgtctgaac cgcctttcag acggcatttc acacatcaaa
     9841 acagaaaaac cgcgcccaaa atcatcatca caaaacacaa cggcaaaccg gatacaaaac
     9901 ccaacaccgc cgccaaaatg ccgtctgaag ccgtcggcgc aaccatcctt atctggggca
     9961 taactttcct taaccatcct tttgacagcc aaataaacag tgcttttgcc tgtcagaaac
    10021 aaaaccaata ttttgcggtt cgtatcggaa catttatagt ggattaacaa aaaccagtac
    10081 agcgt
//
LOCUS       DAWFJR010000069        10070 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWFJR010000069 DAWFJR010000000
VERSION     DAWFJR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10070
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.069"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(137..1354)
                     /locus_tag="WGR04_001536"
     CDS             complement(137..1354)
                     /locus_tag="WGR04_001536"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ0795063.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLTAGKVVIVAGFQGISSEGNISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1556..2284)
                     /gene="rph"
                     /locus_tag="WGR04_001537"
     CDS             complement(1556..2284)
                     /gene="rph"
                     /locus_tag="WGR04_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ0795064.1"
                     /translation="MPDYIRTSRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2366..2830)
                     /locus_tag="WGR04_001538"
     CDS             complement(2366..2830)
                     /locus_tag="WGR04_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ0795065.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3047..3823
                     /locus_tag="WGR04_001539"
     CDS             3047..3823
                     /locus_tag="WGR04_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ0795066.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNIAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVECKQPFVLSGGAGGQKDP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKAKMRVPCVFSTENITPPQSGAAC
                     STDAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            4068..4922
                     /locus_tag="WGR04_001540"
     CDS             4068..4922
                     /locus_tag="WGR04_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ0795067.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            4963..6171
                     /gene="pncB"
                     /locus_tag="WGR04_001541"
     CDS             4963..6171
                     /gene="pncB"
                     /locus_tag="WGR04_001541"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ0795068.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEVELDALCQLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAVRLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6263..7981
                     /gene="argS"
                     /locus_tag="WGR04_001542"
     CDS             6263..7981
                     /gene="argS"
                     /locus_tag="WGR04_001542"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ0795069.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIHAALNDA
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVSEFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGSIKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATAPTVLTEPLEKQLAAELLKFENVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAESASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            8552..9970
                     /locus_tag="WGR04_001543"
     CDS             8552..9970
                     /locus_tag="WGR04_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ0795070.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT         2322 a         2971 c         2535 g         2242 t
ORIGIN      
        1 atttaatcca ctataaattt aatccactat attttttgtt ccaaagtcaa aatatgccgt
       61 ccgaacattc gggcggcaga caaaacggca ctgcccgata aaggcagtgc cgttgtccgt
      121 ttcaaaccgt gaaacatcag cccaaattaa aggctttatg caataccctg gttgccagtt
      181 ccatgtattt ttcatcaatc aatacggaaa ctttgatttc ggaggtggaa atcatttgga
      241 tgttgatacc ctcttcggcg agcgtgcgga agattttggc ggctacaccg acgtgcgaac
      301 gcatacccaa accgactgcg gagactttgc acacggtgtc gtcgccatca atagaagccg
      361 cgccgatact gtcttggcgt tccgacagga tttccaaagt ctgcttgtaa tcgccgcgcg
      421 gtacggtaaa ggaaaaatcg gttgtgcctt cgctgccgac attttggata atcatatcga
      481 cttcgatgtt ggcatcggcg accgcgccca aaatctgata ggcgacaccg ggtttgtcgg
      541 gtacgccgcg cacgttgatg cgggcttggt ttttatcgaa tgcgataccg gttacggcag
      601 ctctttccat gttgtcgtcc tcttcaaagg taattaaggt gccgttgccg ccgtcttgca
      661 ggctgctcag tacgcgcagg cgcactttgt actttccggc gaattctact gaacggattt
      721 gcaagacttt cgagccgagg cttgccaatt cgatcatttc ttcaaatgta accgtatcca
      781 tgcggcgcgc ttcgggtacg acgcgcggat cggtggtgta cacgccgtcc acgtcggtat
      841 agatttggca ttcgtccgct ttgagggctg cggcgagcgc aacggcggaa gtgtcggaac
      901 cgccgcgtcc gagcgtggaa atattacctt cactgctgat gccttggaag ccggcaacga
      961 tgacgacttt gccggcggta aggtcggcac gcattttttc gtcatcaatg ctttcgatgc
     1021 gggctttggt gtgggcggta tcggttttga gggcgacctg ccagcctgtg tagcttttgg
     1081 catccacgcc gatgtctttc aatgccatcg ccaaaaggcc gatggttact tgttcgccgg
     1141 tggacaaaac cacgtccaat tcgcgcggat cgggatgctc ctgcatttcg tgcgccaacg
     1201 cgaccaggcg gttggtttca ccgctcatgg cggatacgac gacgacaatg tcgtgtcctt
     1261 cggcgcgggc tttggcgaca cgtttggcca cgtttttaat gcgttcgggc gagcctacgg
     1321 atgtgccgcc gtatttgtgt acgattaacg ccatgtttcg tgctttcttg tgtgggattg
     1381 gtcgggcagc atggtttgct ggaaaagcgg ctattattac tattttttac atgaagttca
     1441 agaacggact gcgctttccc gcctgccgtt tgacagcggt cagcgaaaaa cctgttcttt
     1501 cagattgttg acaaaatgcc gtctgaacgg ttttcagacg gcatccggac gacaatcagg
     1561 cggcggacaa cgcattttgc tggtgttgca gcagttcgcc tatgcctttt tgcgccagtg
     1621 caaccagttt gcccaattcg tccaaactga acggcgcgtc ttccgccgta ccttggattt
     1681 cgatgatttt tcccgatgcg gtcatgacga tattcacatc actgtcgcaa ccggagtctt
     1741 cgggataatc caaatccaaa agcggcacgc cgttcactac gcctactgac acagcggcaa
     1801 cggcttcgcg gatggggttt tcactcaaaa tgccgtctga aaccagtttg ccgacggcga
     1861 tttgcagcgc gacaaacgca ccggtaatcg aagccgtacg cgtaccgccg tctgcctgaa
     1921 tcacgtcgca gtcaatcaag atttgtcgtt cgccgagttt ttccatatcc acgaccgcgc
     1981 gcagcgaacg cccgataagg cgttggattt cttgtgtgcg cccggactgt ttgcccgccg
     2041 aagcttcgcg gcgcatccgg gaagcagttg aggcaggcag catcccgtat tccgccgtta
     2101 cccagccttg gtttttaccg cgcagaaacg gcgggacgtt ttcatctatg gaagcggtac
     2161 aaatcacttt ggtattgccg cattcaataa ggcacgaacc gtccgtatgc ggcaggaaat
     2221 gaggggtgat tttgatatcg cgcaggctgt cggcggcgcg tgaggtgcgg atgtaatcag
     2281 gcatactgcc ctcccgttaa aaacagataa attaaaaagc cttaaatatg aaaaatcaca
     2341 tttaaggcct tcaaactgaa aatttctacg cctcttcggc tttgctgcgg ataatcaaaa
     2401 gcggcaggtg gctttggcgc attaccgttt cggcaaaact gcccattaaa aggtgcatca
     2461 gcccggtacg tccgtgcgta cccaacacca gcaggtcggc accgttttca tcggcataat
     2521 caaccaaatc ctgcgccatt tcacgcgcac ccttattggc aaccagcagg tgtttgacgg
     2581 tattttccac acccagttcc cgggcggtgc gctcggcggc atccaaaact tcgttgcctt
     2641 gcgcgacggc ggcggcttcg tagctttcgt gttgcaaaaa ttcgggggcg agcgccatat
     2701 attcggcagg attggcaacg tgtaccaaag tcagacgcgc accgttgacc ccggcaagct
     2761 cggcggcatg tttcagggca ttgatggacg tttcactgcc gtcaacggca acaaccagat
     2821 gtttgtacat atcgtattct ccttttgcac cgcctcgcgg tgccctcttg tcggatgggc
     2881 gcagggacag tttgcgccgt ttcattatag acccgccgtc gggctttata caacagccga
     2941 acagcccgac cgctttccag tataatatgc cgcttccgtg cagtcaggca ttttttgccg
     3001 gctttcgttc actttttgat ttgacgcaat cttgcaggat tcgaccatgt ccgacaacgc
     3061 tttgacctct tcgcgacgct tcggcggcat cgccagactc tacggagact ctgccttggc
     3121 gcacttttca caggcacacg tctgcgtggt cggcgtgggc ggtgtcggct cgtgggcggt
     3181 cgaggctttg gcgcggacgg gcatcgggcg tttgactttg attgatttgg acaacattgc
     3241 cgaatcgaat gtcaaccgcc agctgcacgc cctgaccggt gacttcggca aagcaaaagt
     3301 taccgccttg cgcgaacgta ttacacaaat caatccgcaa tgcgaagtat ttgaaattga
     3361 agatttcgtt accgaagaca atttgccgga atacttcgga aaaggttttg atttcgtcat
     3421 cgacgcgatc gaccaagtgc gcgtcaaagc ggcaatggcg gcttattttg tggaatgcaa
     3481 acaaccgttt gtcctcagcg gcggcgcggg cggacaaaaa gatccggcgt tgatccaaac
     3541 cgccgatttg agccgcgtaa cccacgaccc gctgcttgcc aacctgcgct acaccttgcg
     3601 gaaacgctac ggcttcagcc gcgatacgaa agccaaaatg cgcgtgccgt gcgtgttctc
     3661 aaccgaaaac atcacgccgc cgcaatcggg cgcggcgtgt tctaccgatg ccgctccgca
     3721 aggcttgtcg tgcgccggct acggtgcaag catgctcgtt accgcttcgt tcgggctata
     3781 ttgcgcacag gcggcggtgg aacacatcgc agacaaaaaa taagcaatgc cgtctgaaac
     3841 aggattcaga cggcatttga acaaacgcgg caatcaagct gtcggttcag aaaaaccaac
     3901 atcggcacgc ctaaaaccgt attcacaacc tgctcctttt caaaacattt gcatttaaaa
     3961 gccgttataa tgccgtctga acatctgccc gaccacatta tacgtgaatg tcggcagatt
     4021 gttttctttt gtaaacttat attaaaatcc acttaccgat tcacgccatg ccgcccatcc
     4081 ctgccccatc cgcaccatcc gagcacactg tcgcatgggt attcggccaa cccgttaccg
     4141 atttgccgca ggatttgttt atcccacccg acgcgctgaa agtcgtattg ggcagttttc
     4201 aaggcccttt ggatctactg ctgtatctga tccgcaagca gaacatcgat gttctcgata
     4261 ttccgatggt gaagattacc gagcagtatc tgcactacat cgcccaaata gaaacctatc
     4321 agtttgattt ggcggcggaa tatcttttga tggcagcaat gctgattgaa atcaaatcgc
     4381 gcctgctgct gccgcgtacc gaagccgtcg aagacgaaga agccgacccg cgtgccgagt
     4441 tggtgcgccg cctgttggct tatgaacaga tgaagctggc ggcacaaggg ttggatgcgc
     4501 ttcctcgtgc gggccgggat ttcgcgtggg cttacctgcc gctggaaatt gccgtcgaag
     4561 ccaagctgcc cgaagtctat attaccgact tgacgcaagc gtggctgggt attttgtctc
     4621 gggcaaaaca cacgcgcagc cacgaagtaa tcaaagaaac catctccgtg cgcgcgcaaa
     4681 tgacggcaat cctgcgccgt ttgaacggac acgggatatg caggtttcac gacctgttca
     4741 atcccgaaca gggcgcggct tacgtggtcg tcaacttcat cgcactgttg gagcttgcca
     4801 aagaaggttt ggtcggaatc gtacaggaag tcggtttcgg agaaatccga atcagcctca
     4861 atcatgaggg ggcacattca gacggcattt ccggcacacg gggcgggcgc gatgtgttct
     4921 aatacgcccc aagccgccac caaaaatcgg gagacacgcc atatgaccgg catcatacat
     4981 tcgctgcttg acaccgacct ctacaaattc actatgctgc aagtggttct gcaccagttt
     5041 ccgcagacgc acagccttta cgaattccgc tgccgcaacg cctcgaccgt ctatccgctt
     5101 gccgacatca aagaagactt ggaagtcgaa ctcgacgcgc tctgccaact gcgctttacc
     5161 catgacgaac tcggctacct ccgcagcctg cgtttcatca aaagcgactt tgtcgattat
     5221 ctcgaactct tccagctcca acgccgcttt gtcgaaatcg gcacagacga taaagaccgt
     5281 ctgaacatcc gcatcgaagg tccgatgata caggcgatgt tttttgaaat cttcatcctc
     5341 gccattgtca acgaacttta cttccgccgc ctggaaaccc ctgcagtcat agaagaaggc
     5401 gaacgccggc ttcaagccaa agccgtgcgc ctcaaagaaa tcgccgccgc acaaaacccc
     5461 gacgaaccgc ccttcctgat ttccgacttc ggcacgcgcc gccgctacaa gctcgcgtgg
     5521 caggaacacg tcatccgcac cctgctcgaa gccgccccaa gcatcgtgcg cggcaccagc
     5581 aatgtctttc tcgccaaaaa actcggcatc acccccatcg gcaccatggc gcacgagttc
     5641 ctgcaggcat tccaggccct cgacgtacgc ctgcggaatt tccaaaaggc cgcgctcgaa
     5701 agctgggtgc acgaataccg gggcgatttg ggcgttgccc tgaccgacgt ggtcggtatg
     5761 gatgccttcc tgcgcgattt cgacctctat ttcgccaaac ttttcgacgg gctgcgccac
     5821 gacagcggcg acccttacgt ttggggcgac aaagcctacg cccactatca aaagctcaaa
     5881 atcgacagcc gcaccaaaat gctgaccttc tccgacgggc tggacatcga acgctcttgg
     5941 gcattacacc aatatttcaa agaccgcttc aaaaccggct tcggcatcgg caccaacctg
     6001 accaacgata tggggcatac gcctttgaat atcgtcttga aactggtcga atgcaacggg
     6061 cagtccgtcg ccaagctgtc cgactctccg ggcaaaacca tgaccaacaa cagcaccttc
     6121 ctcgcctacc tgcgccaagt gttcgacgta cccgaacccg aaacgccgta aaccggcaga
     6181 aaaagcgcac aattcctgtt tctgccgcat aaaatctttt aaaataccgc ctgatttgaa
     6241 tttaaccgaa agaccgaact tcatgaacct acatcaaacc gtcgaacacg aagccgccgc
     6301 cgcctttgcc gccgcaggca tcgccggcag ccccgttgtc ttgcagccga ccaaaaatgc
     6361 cgaacacggc gatttccaaa tcaacggcgt gatgggtgcg gcgaaaaaag ccaaacaaaa
     6421 cccgcgcgaa ctggcgcaaa aggtcgccga cgcattggca ggcaacgccg tcatcgaaag
     6481 cgcggaagtc gccggtcccg gttttatcaa cctgcgcctg cgccccgaat ttctcgcgca
     6541 aaacattcat gcggccttga acgacgcgcg tttcggcgtg gcaaaaaccg acaaaccgca
     6601 aaccgtcgtt atcgactatt cctcgcccaa tctggccaaa gaaatgcacg tcggccatct
     6661 gcgttccagc atcatcggcg acagcatttc gcgcgtgtca gaatttatgg gcaataccgt
     6721 tatccgtcaa aaccacgtcg gcgactgggg tacgcaattt ggcatgttgg tcgcttattt
     6781 ggtcgagcag caaaaagaca atgccgcgtt cgagctggcg gatttggagc agttttaccg
     6841 cgccgccaaa gtgcgctttg acgaagaccc tgcctttgcc gacaccgcac gcgaatacgt
     6901 tgtgaagctg caaggcggcg atgaaaccgt tttggcattg tggaaacagt ttgtcgatat
     6961 ttcgctctcg cacgcccaag ccgtttacga cacgctgggc ttgaagctgc gccccgaaga
     7021 cgtggcgggc gaatcgaaat acaacgacga tttgcagccc gtagtcgatg atttggttca
     7081 aaaaggtctg gcagtcgaag acgacggcgc aaaagtcgtg ttcttggacg agtttaaaaa
     7141 caaagagggc gaacctgccg catttatcgt gcaaaaacaa ggcggcggct tcctttatgc
     7201 ttccaccgac ttggcgtgtt tacgctaccg tgtcggcacg cttcatgccg accgcctgct
     7261 gtacgtcgtc gaccaccgcc aagccctgca ctttgagcaa ctctttacca cttcacgcaa
     7321 agccggctat ctgccggaaa acgtcggcgc ggcatttgtc ggcttcggca cgatgatggg
     7381 caaagacggc aaaccgttca aaacacgcag cggcgacacc gtgaaactgg tcgatctgct
     7441 gaccgaagcc gtcgaacgcg ccaccgcttt ggtgaaagaa aaaaatcccg aattgggtgc
     7501 ggacgaagcc gctaaaatcg gcaaaaccgt cggcatcggc tcaatcaaat acgccgattt
     7561 gagcaaaaac cgcaccagcg attatgtgtt cgactgggac gctatgcttt cgtttgaagg
     7621 caacaccgcc ccctacctgc aatacgccta cacccgcgtg caaagcgtgt tccgcaaagc
     7681 aggcgaatgg gacgcgaccg cgccaaccgt tttgaccgaa cctttggaaa aacaactggc
     7741 cgctgagctg ctgaaatttg aaaacgtgct gcaaagcgtg gcggacacgg cgtatccgca
     7801 ctacctcgcc gcctacctct atcaaattgc gaccctgttc agccgcttct acgaagcctg
     7861 tccgatactc aaagccgaaa gcgcaagccg caacagccgc ctgcaactgg cgaagctgac
     7921 cggcgacacg ctgaaacaag gcttggattt gctgggcatc gatgtgttgg acgtaatgta
     7981 aaaccgcacc gcccgattgc ggacaacagc cccaccatcc ttatccgaat ctgaaaaaag
     8041 cggcgcgata caccgtatcc gccgcccctc ccaaaatgcg aaacaaacaa acgccaagca
     8101 agcaagcaag caagcaagca agcaagcaag caagcaagca agcaagcaag caagcaagca
     8161 aaaaattata acccccctta ctgccgacgc acgcactttc cgcgcggcgc attccccttt
     8221 tcccgcccct caaacccgcc ttttcttcag gcagggtttc agcccacctc ctttccctgt
     8281 tttcctttcc ccgacacgca tgcgcgcccc ctgccgcacc gtgccgcatt ttcgcgcccg
     8341 gacggcatcg ttccgccatc cggttctctg ttttaaatac ccctgtttca gaaagaaatg
     8401 cagatgtttc aacacacagg acgacacata aagcaccgcc ctatgtgttg ccctgatttg
     8461 gaaggggtta cgcctcccaa ataaagtctg atcctgccgc cccgaaggac agatgtccga
     8521 gtggcgaagt ttcaaccgaa aaggaaatac gatgaatatt cacaccctgc tctccaaaca
     8581 atggacgctg ccgccattcc tgccgaaacg gctgctgctg tccctgctga tactgcttgc
     8641 ccccaatgcg gtgttttggg ttttggcact gctgaccgcc accgcccgcc cgattgtcaa
     8701 tttggactat cttcccgccg cgctgctgat cgccctgcct tggcgtttcg tcaaaattgc
     8761 cggcgtattg gcgttttggc tggcggtttt gtttgacggg ctgatgatgg tgatccaact
     8821 cttccctttt atggatctca tcggcgccat caacctcgtc cccttcatcc tgaccgcccc
     8881 cgccccttat cagataatga ccgggctgtt gctgctgtat atgctggcga tgccgtttgt
     8941 gttgcagaaa gccgccgcca aaaccgactt ccggcacatt gccgtctgcg ccgccgttgt
     9001 ggcggcagcc ggctatttca ccggccattt gagttactac gaccggggtc ggatggccaa
     9061 tatcttcggc gcaaacaact tctactacgc caaaagtcag gcgatgctct acaccgtcag
     9121 ccagaatgcc gactttatta ccgccggcct ggtcgatccc gtcttcctcc ccttgggcaa
     9181 tcaacagcgt gccgccacgc atctgaacga gccgaaatct caaaaaatcc tctttatcgt
     9241 cgccgaatct tgggggctgc cggccaatcc cgaacttcaa aacgccactt ttgccaaact
     9301 gctggcgcaa aaagaccgtt tttcggtttg ggaaagcggc agttttccct ttatcggcgc
     9361 gacggtcgaa ggcgaaatgc gcgaactgtg cgcctacggc ggtttgcgcg ggttcgcact
     9421 gcgccgcgcg cccgacgaaa aatttgcccg ctgcctcccc aaccgtttga aacaagaagg
     9481 ttacgccacc tttgcgatgc acggcgcggg cagttcgctt tacgaccgct tcagctggta
     9541 tccgagggcg ggctttcaag aaatcaaaac cgccgaaaac ctgatcggta aaaaaacctg
     9601 cgccattttc ggcggcgtgt gcgacagcga gctgttcggc gaagtgtcgg catttttcaa
     9661 aaaacacgac aagggactgt tttactggat gacgctgacc agccacgccg actatcccga
     9721 atccgacatt ttcaaccaca ggctcaaatg caccgaatac ggcctgcccg ccgaaaccga
     9781 cctctgccgc aacttcagcc tgcacaccca attcttcgac caactggcgg atttgatcca
     9841 acgccccgaa atgaaaggca cggaagtcat catcgtcggc gaccatccgc cgcccgtcgg
     9901 taacctcaat gaaaccttcc gctacctcaa acaggggcac gtcgcctggc tgcacttcaa
     9961 aatcaaataa caacaatgcc gtctgaacgc accaacagcc ttcagacggc attttgcaga
    10021 cagaccgacc cttcaagccc acttttttca tcatctccga taaattgctt
//
LOCUS       DAWFJR010000070         9845 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWFJR010000070 DAWFJR010000000
VERSION     DAWFJR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9845
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.070"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(123..818)
                     /locus_tag="WGR04_001544"
     CDS             complement(123..818)
                     /locus_tag="WGR04_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0795071.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(933..3041)
                     /locus_tag="WGR04_001545"
                     /pseudo
     CDS             complement(933..3041)
                     /locus_tag="WGR04_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(3227..3832)
                     /locus_tag="WGR04_001546"
     CDS             complement(3227..3832)
                     /locus_tag="WGR04_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0795072.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVVGGIGAPAGEKTLQPVAERLKVFGA"
     gene            complement(3880..4278)
                     /locus_tag="WGR04_001547"
     CDS             complement(3880..4278)
                     /locus_tag="WGR04_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0795073.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            4386..5297
                     /locus_tag="WGR04_001548"
     CDS             4386..5297
                     /locus_tag="WGR04_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0795074.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            5287..6588
                     /locus_tag="WGR04_001549"
     CDS             5287..6588
                     /locus_tag="WGR04_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ0795075.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCAAVAEGLPI
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRTV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPPPAALSDGIFLPQNGGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGQTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWWLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAYPVAGIP"
     gene            complement(6656..6880)
                     /locus_tag="WGR04_001550"
     CDS             complement(6656..6880)
                     /locus_tag="WGR04_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795076.1"
                     /translation="MVFWLQRLKYPLLLWIADMLLYRLLGGAEIECGRCPVPPMTDWQ
                     HFLPAMGTVSAWVAVIWAYLMIESEKNGRY"
     gene            6983..9133
                     /gene="dinG"
                     /locus_tag="WGR04_001551"
     CDS             6983..9133
                     /gene="dinG"
                     /locus_tag="WGR04_001551"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ0795077.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALLDRLTGEFGIFIVRIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAIIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHQAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            9170..9783
                     /locus_tag="WGR04_001552"
                     /pseudo
     CDS             9170..9783
                     /locus_tag="WGR04_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
BASE COUNT         2291 a         2894 c         2544 g         2116 t
ORIGIN      
        1 ccgctatata ttctgattta aaaccataag gctttaagca atcatctctt ctataaagcc
       61 taaatacaaa aggccgtctg aaatcctatt ttcagatggc ctttactcat tcaatcctca
      121 acttattgcg gctttttctg ctcttcgcgt actttatcca ccaactggtc aatcgtggtc
      181 ataccggact gccagtcttt aaattcaact tggtatttgc cgccgacaat cacagtcggt
      241 gtgccgctga tttggaattt attggtcaac tcttccattt gagccgcacg cgcttggctt
      301 tcaggagcct caaatgcagc caatactttt ttgccgtcaa acgctgtttg ctcggacagc
      361 cattttttca gggtatcggt atcggccaga ttgatttttt gattaaccat cgcatcgaaa
      421 atatggctgt tggctttatc tgattcaccg gccatttcca ctgcggccgc caaacgtgcc
      481 aaaggtttca tttcatcacc ccacacgaca tgctcccggc gcatataggt atcgtcttta
      541 aacgttttga tgtgctcgct caagaccggc tcaagatggg cgcaatgcgg gcagaagtag
      601 ccgaaaaatt ccaatacttc gattttaccg gcctgctgtt gcggaatagg cgtagacaat
      661 acagtgtagt tcacaccttc gttcaactca gcaggggctg ccggagcaga tgagctgctt
      721 tgggcgctgt ctgccggaac actggtttca gcctgtttgc tacaagcggc caatgccaac
      781 agggtcaatg aagtcaaagc taaggttttc agtttcatag gtatctcttg tgtgtcagat
      841 taatgtgcgg attttatggc attttattga aggcgtgttc gatttgagtg aaaaaatgtg
      901 tttagtttta tttcagcctt tgcctgattt catcagaaac ggtaatttac ccgggcagta
      961 tagccgcgcg gattacccgg catagagtcc gaacgccaat atttttgatt gaacagattg
     1021 gctgcggcaa aggtaacgtt aacattttta tggttccagc cgagcatggc atcggtacgg
     1081 gcgaagccgg gaagcgtggt ggttgctttt tggctggagt tgtagccgta gcgtttgccc
     1141 gtaccggtta cgccgatttc gccgtagagg ttttcggtcg gggtataacg gaaaaacagg
     1201 ttgccggtaa cgttgctggt attattcaaa tggatgccca ctcggtcggg attttctttg
     1261 tcttcaacga ctttcgcctg catcacgccc aacgaaccgc gcagatagag ttttttaggg
     1321 atgatttgcc cgatggcgga caattccacg ccgcgcgaac ggtgtttgcc gctaaccgca
     1381 taaatataag ggttgttttt tggatcgggg cggtagcgga tattgaagcg ttcgatttgg
     1441 taggcagaca acgtagtgct gaggcggtcg tccagccaac tgcttttcac accggtttcg
     1501 tattggcggg tgtactcggg gtcggcgttg aacacggcgg aagacgacgt gttgatgctc
     1561 aaatagccgc gtccgccata aggcgcgaag cctttgttat acgaggcgta aagtgtgtgg
     1621 actgggttga tgttccacac tgcgccgatg ttggggctga acgagtggcc gctgtattgg
     1681 cggctgttgc cggtgagttt gttttcggaa ttaaaggtgt atttgtcgta acggccgccg
     1741 aggacgaatt tcaaatcggg cgtagcggag aagatgtttt gcacaaagat gccgtaggag
     1801 tcggctttgt ggcggttttg ggtcagaata ggctgcaatc tgcccgaagc cggccagctt
     1861 gcgcggtcgt aggggttgat gggcacggtg aaactgccgc ggtagcccaa tgtcgggttg
     1921 cggtgttcgc ggctgtaatc catgcctacg gtcaagtggt tttcaaaacg gccgatggcg
     1981 tagtcgccgt ttagcgtgaa attggacgac agggttttgt tgtcggtctg ctgccaggcg
     2041 tagttacgtt tgattaagtt gccattttcg ctgcctgcat agaaatgatc aaaatcctgc
     2101 gccgccgtgc ggtgggcgag ctgccattgg gcacgccatt tgtcgttgaa ggcgtattca
     2161 aggtcggaac gccaaacttg cagcttgtct ttgacaaaat cgttccggtg ggcgaacccc
     2221 atgcggtaag gcagtccgaa gcggtcgtac acggacttgg tcggactgcg gtcgggcgtg
     2281 cgctccacat tgtcgtaggt gtattgcccc gtccacttca agccgttgtc gagtttgacg
     2341 gtaatgctgg gcgaaaccat gacatttttg ctgtctatgc cgctgcggaa cgaattggcg
     2401 cgcccgactt cgccggtgag acggatggcg acgtttttgt tcagcacttc gttaatgtcc
     2461 atattcaggc tgcggtttgc ccacgaaccg taaaccgcac cgatgttgcg gctttgtttg
     2521 aagttggcgt atttgctgac catgttgatg acgccgccgc cgttggtgcg gccgtaaagc
     2581 acggaagacg ggcctttcag gatttccacg cgctcgatgt tggcagtact gcggcgcact
     2641 tgtccgcttt cgcgcacgcc gtcgcggtaa atatcggatg cgtcggcttg aaaaccgcgc
     2701 aggaaaatgc tttcgccgcg catatcgtag gcagcgtcga tgccggcatt gccttagagg
     2761 atggaactca aatcgttcgt accgtaattt ttgtttttct ggatattgag cgtatcgatg
     2821 gtttgcggcg tttctttgat gagctgtccg ttgcgggtaa cggcggcttc gtcgtagttg
     2881 atgtagcctt tgagtacgct ggtgtcggac tgtccgacca cggaaacggt gggcagagtg
     2941 gcggtgtaat gttcaccatt gtcctgcgta tcggcggcag caacagggaa ggaagcaata
     3001 atcagcgtgg gtaataaagc taaatgaaat gatattttca tttttatact caatttaaca
     3061 aaacaaccga attatattgc ctcacggagg aaatgagaat aatttctttt aactatattg
     3121 aacatgatat ttgtaaacaa aggtctcaga atgcggaaaa ctcgccgcct gatactgaaa
     3181 aatgccgtct gaacagggtt cagacggcat ttttttgacc gcgaaattat gcgccgaaca
     3241 ctttcaaacg ttctgcaacg ggttggagcg ttttttctcc ggcgggcgca ccgattcccc
     3301 cgacaaccat ttgtgcgcgc aacaaccagt tttcggggat attccacgct ttggcaatcg
     3361 ccgcatcggg caagggattg taatgttgca ggtttgcacc tacgccgacc gcggcaagtg
     3421 tcgtccaaac ggcatactgc accatcgcgt ttgcctgatc cgcccaaacg gggaagttag
     3481 cggcataagc agggaactgc tcctgcaaac ctttgacgac attttgatct tcataaaaca
     3541 aaatggttgc cgcacccgcc ttaaacaggt tcaatttttg cgcggtcggt tcaaaactgt
     3601 cggcaggcac gacggcacgc agcgcgtctt cgacaaattg ccacacctta tcatgctctt
     3661 cgccaaacag cacgaccacg cgggcagatt gggaattgaa cgaagaaggt gtgtgcaaaa
     3721 cggcgtgttc gacgatttgg acaacttcat ctttgccgac gggcagattt ttatttaacg
     3781 aataaatgga acggcggctt tcggcagcct gttgcagaga ttgacgggtc atttcttttc
     3841 ctcaaaaaca ttcaacaaaa tacactttag gcagtccgat caggctttac gcttgaaaaa
     3901 cagcctgtcc agcgaccatg ctccgccgcc cgctgccgcg atatagagga atacgaagca
     3961 gaacagcact gcggactcgc cgccgttggc aatcgggaac aaagcatttc cggaagcgtg
     4021 cgccataaaa taggcaaccg ccatctggcc ggacaaaaca aacgcggcag ggcgcgcaaa
     4081 caggcccaac accagcaaaa tgccgccgac aatttctaaa ataccggcaa gcagcaacag
     4141 cccgccgggc gaaccgctgc ccatttcaat ggggaaggcg aagattttcg acgtaccgtg
     4201 caacaaaaac aggtaggcgg ttacgatacg caaaacagaa agcaaaaccg gttggatacg
     4261 gttgcagcaa tcggacataa aagctccttt gatatttgtc ttcatttcag gcggtagagt
     4321 atagatgttt ccccttttca aatatatact gcttttattg atacaacttt tccacacaag
     4381 aaaatatgga caccctgttc agcctcaagg ttttccgcca agtcgtccaa agcggcggct
     4441 tcacccgcgc cgccgacgcg ctcggcatct ccaccgcaat ggcaagcaaa cacgtcagcc
     4501 acttggaaaa caccgtccaa gccaaactcc tgcaccgcaa cagccgcaac ctcagcctga
     4561 ccgaagccgg ggaagaatac taccggcaat gcagttacgc gctcgacacg ctcgacgatg
     4621 ccgcccaaaa agccgccggg gggacggaaa aaccgcaggg gctgctgcgc gtaacgatgc
     4681 cgctgtggtt tgccggcagc cagatatgca actggctggc ggaataccgc gaacgttatc
     4741 ccgaagtaac attagaactg attttggaca accgccacgt cgatttgatt gccgaaggcg
     4801 tggatttggc gttgcgcgtt tcccaaaccc tgtccccgtc gctgattgcg cgcccactgg
     4861 cggaaatcga atttgccctg ctcgcctcgc ccgatttcct gaggcgcaac ggcgtgccgg
     4921 aaacgccgga agaagtggcg gggctgcccg ccgtcctgcc gacctacacc aaccagcaga
     4981 aactcgacct cacccgcaaa tcggacggca aaaaataccg gcttgaactg acccccgtca
     5041 tccgtaccga caacacgctg atgatgcgcg aaatgattaa ggcgggcgcg tgcatcggtt
     5101 atcagccgct ttgggcggcg gaacacgatt tgcgctgcgg cacgctggta aggctgctgc
     5161 ccggatacgc cgtcccgacc gaccggctga atgccgttta tgcagacagg gcgttcttaa
     5221 gcgcgaaagt ccgtagcttc atcgattttc tgaacgaaaa aatcgccagc aggaaaggct
     5281 gccgaaatgc cgtctgaaac cgcccgcccc ctttatgcgg acacgtgcgc cgcacacacg
     5341 ctcgtctggt tccgccaaaa cctccgcatc cgcgacaacg ccgccttatg cgccgccgtt
     5401 gccgaaggtt tgcccattat cggcatttgg attgacgatg ccgaaacaga caaccctcgc
     5461 cgcgccgcgt tctaccgcca atccgccgcc gaactcgccc aagggcttgc agggcgcggc
     5521 atcccgctct acacggcggc atcgcctgcc gagctcgtcc ggctcgccgt ccgcctcaat
     5581 atccgcaccg tcatcgccga cgaatcccat acttttgccg acaaactcgc cgacaacgcc
     5641 ctttggcatg aattggacaa acacggcatc gcgttaacct tcgtcaatga ccgcgccgtt
     5701 ttcggcaaaa ccgacctgat acccgacggc ggcacggcac acaccgattt cgaccgctac
     5761 cgcgaagtat ggctcgaccg cttttccaag cagccccccg ccggtccgga cctattcgcg
     5821 gcataccgcc aacccttccc cgaaaacctt tccgccccgc cgcctgccgc cctttcagac
     5881 ggcatcttcc tgccgcaaaa cggcggcgaa acggcggctt ggcggcagtg gcggcggttt
     5941 ctcgaacagg cggattccta ctccgtttta aaggatttcc cctcacgcaa acacacttcg
     6001 ctgatgggcg cgtatttgag tgccggctgc atctcgccgc gcctgctcgc acgggaaagc
     6061 ctcgaacgcc gtctgaatgc gtgggcggac aacatcatcc gccgcgattt tttccttcaa
     6121 cttgccttgc agcacacgga tgacgaccct tcagacggca atcctgaaca caccctgcgc
     6181 ctgacgcttt ggcagcaggg ccagaccggc attccgatta tcgatgccgc gatgcgctgt
     6241 ttgcacaaaa ccggcagcct ccaccccgcc ctgagacgct tgagcgcgga ttttttctgc
     6301 cacgttttaa acctcccccg ccgcgaaggc gagatatggt ttgcccggca gctgaccgat
     6361 ttcgatgcgg caatcaacca aggcaactgg tggcttgccg cctcacggca cacctgcccc
     6421 gacattgccg ccgcctcata ccgtaccgac ccggacggta cttttatcaa acggcacatt
     6481 cccgaactcg cccacctgtc cgccgacacc gtccacacgc cttggcggtt tgcctgttcg
     6541 gtcgataccc acggctatcc cgcctatcct gtcgccggta ttccctaagc ggtacggcaa
     6601 cctaaaacaa atgccgtctg aacttccgtt tcagacggca ttgcgtccga atgactcaat
     6661 atcttccgtt tttttcactt tcaatcatca ggtatgccca aatcaccgcc acccaagccg
     6721 acaccgttcc catcgccggc aaaaaatgct gccaatccgt catcggcggc acagggcaac
     6781 ggccgcattc gatttccgcg ccgcccaaca accggtacag cagcatatcc gcaatccaaa
     6841 gcaacaacgg gtatttcaaa cgctgcaacc aaaaaaccaa ccacgtccgc acgattcacc
     6901 tcacccccca ttccgacaac gatattgtac cgcttccgtg caatgttaaa ataagcccac
     6961 ttctaccgcc gtacaaaacg ccatgctcac cgatttagaa aaaaacgcca tccgcgacca
     7021 ttaccaaaac atcggcaaaa acctgcccgg tttccgtccg cgcgcttcgc agcgggaaat
     7081 gattgcggcg gttgccaacg ctttttcgcg gacgttggcg cgtgaagaag gcggcgagcc
     7141 gcccaagcgc gagggcgaga gcatcgccgt aatcgaaggg ccgaccggcg tgggcaaatc
     7201 gcttgcctac ctgctggcgg gcggcatcat ggcgcaaaca cgcggcaagc ggctgattgt
     7261 gagcagcgcg acggttgcct tgcaggagca gttggtagac cgcgacctgc cgtttctggt
     7321 cgaaaaaagc ggtttggaac tgaccttcgc acttgccaaa gggcgcggcc gctatctctg
     7381 cccctacaaa ctctatcaac tgacgcaaag caatgcccag caaaacctgc tcggctttga
     7441 agcccccgcc gtcttgtggg acagcaaacc caagcccgaa gaattgaagc tgctgcgcga
     7501 catcgccgac gaattttccg cccgacggtt caacggcgac cgcgacactt ggccggaaaa
     7561 aatcgatgac gcgatttggc tcaaagtgac caacgaccgc cacggctgcc tgaaaaccgc
     7621 ctgtcccaac cgtccggaat gtccgtttta cctagcacgc gatgtcttgg aaaccgtcga
     7681 tgtcgtcgtt gccaaccacg atcttctgct tgccgacatc agtatgggcg gcggcgtgat
     7741 tctgcctgcg cccgaaaaca gtttctattg catagacgaa gcgcaccacc tgcccaaaaa
     7801 agccctcagc cgttttgccg ccgaacattc atggaatatt gccgtttgga cgctggaaaa
     7861 actgccgcag ctgaccggca aaattgccgc gcttaccgat aaagccgaac ttgccaacct
     7921 ggccgacgaa gccgccgcat ccttgctcga cagcctgcac gaatggcagt tccatttggc
     7981 ggaagagccg tctttaagta tggggccgtc tgaaaacgac agacgaacca acagcgaacc
     8041 gacttggctg tgggaagacg gcaaaatccc cgaaggcctc gaaaccaccg tttccaatac
     8101 ggccattgct gcgcgcagcc tgctcaaaca cgttgtcggg ctgaacgatg cgctttccgc
     8161 cgcgcgccgc gaaaaagaac aggacggcgc gctcctcgac cgcctgaccg gcgagttcgg
     8221 catcttcatc gtccgtatcg aacaaatcag cgcggtttgg gatttgctct ccaccgtccc
     8281 cctcgaaggc gaagagccgc tggcaaaatg gataacccgc cgtgccgacg acaaaaacga
     8341 ctacattttc aacgccagcc ccatcagcag cgcatcccac cttgccaaca gcctgtggcg
     8401 gcgtgcggca ggcgcggtat tgacttccgc caccctgcaa tccttgggca acttcaacct
     8461 gatgctgcgc caaaccgggc tgcaatggct gcccgaaacc accaccctcg ccctcaaaag
     8521 cccctttgac tttgaaaaac agggcgaact ctacatcccc cccatatacg ccagccccaa
     8581 agaccccgaa gcccacaccg ccgccatcat cgaatggctg cccaagctta tttcgcccac
     8641 cgaagccatc ggcacgctcg tcctgttttc ctcgcgcaaa caaatgcagg atgtcgccct
     8701 gcgcctgccc ggagactacc tgccgctctt gctcgtacaa ggcgaattac ccaaagccgt
     8761 cctcctgcaa aaacaccacc aggccataga agaaggcaaa gccagcatca tcttcggact
     8821 cgacagcttc gccgaaggac tcgacctgcc cggcaccgcc tgcgtgcaag tcatcatcgc
     8881 caaacttccc ttcgccatgc ccgacaaccc catcgaaaaa acccaaaacc gctggataga
     8941 acagcgcggc ggcaacccct tcatcgaaat caccgtcccc gaagccggca tcaaactcat
     9001 ccaggccgtc ggccgcctca tccgcaccga acaagactac ggccgcgtaa ccatcctcga
     9061 caaccgcatc aaaacacagc ggtacggcca acaattattg gccggcctgc cgccgtttaa
     9121 aaggataggg taaacatacc gcccttcccg caacagaaag gaaaaattca tgaactttac
     9181 ccgactgctc aaccaagtct taagcacggt tcaaaaaaag gcaacacatt ctccgacagc
     9241 ccgctcaatt cattcggcgg aggcgcgctg gttgccggtg tcgcctccat gctgctgaac
     9301 ggtaaaaacc gcaaaaccat caccaaaatc ggttcgaccg ccgctttggg ctacctcgcc
     9361 taccggggct atcagatgtg gcagcaaaac aaagggcggg caaccgtaac acaaagcgat
     9421 ttccaacctg ccggagaaac cgaagaaaca tacagccgca ccgtattgcg caccatgata
     9481 gccaccgccg cttcagacgg catgatagac gaagccgaac gccggactat cgaacaggaa
     9541 agcggcacag accccgaaac tgctgcatgg ctcgccgccg aataccgcct gcccgcaagc
     9601 atcgaagaca tcgccgccgc cgtcggcaac gatgaggcgt tggcggcgga ggcctatctg
     9661 gcggcaagac tggtatgtgc cgatttgtcg cggaaagaaa ccgtcttcct cgcccgcctg
     9721 tcgcaggctt tggaactgga tgacaatctg gtggagagtt tggaaaggca attggggttt
     9781 tgatgacaca gccggggagg gaaaatcatc aaaaaacggt tattccgacc ccggcctcaa
     9841 aacgg
//
LOCUS       DAWFJR010000071         9821 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWFJR010000071 DAWFJR010000000
VERSION     DAWFJR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9821
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.071"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(379..4548)
                     /locus_tag="WGR04_001553"
     CDS             complement(379..4548)
                     /locus_tag="WGR04_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ0795078.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGAAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRINPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIANAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNSKGK"
     gene            complement(4603..6432)
                     /locus_tag="WGR04_001554"
     CDS             complement(4603..6432)
                     /locus_tag="WGR04_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ0795079.1"
                     /translation="MMIKPTALLLPALFFFPHAYASAADLSENKAAGFALFKNKSPNT
                     ESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAPDNVKTM
                     LRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEYYRNALE
                     NWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATADLNVVVDS
                     GRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHYSGASVQ
                     ADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKGYIGSVV
                     WDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVRDRAGID
                     ARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDGKIGTTL
                     GTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTLARDNADVPSGLMFRSGGA
                     SSVRCYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDAAANFKR
                     MKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6826..8055
                     /gene="sstT"
                     /locus_tag="WGR04_001555"
     CDS             6826..8055
                     /gene="sstT"
                     /locus_tag="WGR04_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ0795080.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8167..9450
                     /locus_tag="WGR04_001556"
     CDS             8167..9450
                     /locus_tag="WGR04_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0795081.1"
                     /translation="MFERSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPVVAE
                     KETEVKEDAPQAGSQGQGAPSTQGSQDMAAVSAENTGNGGAATTDKPKNEDEGPQNDM
                     PQNSAESANQTGNNQPADSSDSAPASNPAPANGGSNFGRVDLANGVLIDGPSQNITLT
                     HCKGDSCNGDNLLDEEAPSKSEFENLNESERIEKYKKDGKSDKFTNLVATAVQANGTN
                     KYVIIYKDKSASSSSARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGN
                     IFAPEGNYRYLTYGAEKLPGGSYALRVQGEPSKGEMLAGTAVYNGEVLHFHTENGRPS
                     PSRGRFAAKVDFGSKSVDGIIDSGDGLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGK
                     FYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9525..9740)
                     /locus_tag="WGR04_001557"
     CDS             complement(9525..9740)
                     /locus_tag="WGR04_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795082.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLKAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(9733..>9821)
                     /locus_tag="WGR04_001558"
     CDS             complement(9733..>9821)
                     /locus_tag="WGR04_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ0795083.1"
                     /translation="VTMEKDLRRMLLFFKREAYVVILERDRV"
BASE COUNT         1958 a         2515 c         3038 g         2310 t
ORIGIN      
        1 atgtaggttt tcttaaccct gcgtcctaga ttcccgcttt tgcgggaatg acggaaagtg
       61 gcgggaatga cggttcgggc attccttaaa ttacctgtgt atcgttgtaa atcttagaga
      121 tggcggaata tagcggatta acaaaaacca gtacagcgtt gccttgcctt ggctcaaaga
      181 gaacgattct ctaaggtgct caagcaccga gtgaatcggt tccgtactat ttgtactgtc
      241 tgcggcttcg tcgccttatc ctgatttttg ttaatttact atatcgattc tgttcccttg
      301 caggcggacg gcggcggcgg acggtggaaa agcaggtttt caaatgtccg gtttggcggc
      361 gcgccgtctg aaaaccgctt attttccttt gctgtttccg gcggagtctt ttttgtccga
      421 accggagaag cggtcgaaac gtatggtgta tgtcagctcg ccgcccgacg aacggctgcc
      481 gatacgggca accgcctgta tggcgcgggt cagccggtaa atcagtttga cggactgttc
      541 cgcgctggag atgctgtatt cgtagccgat gtagagtttg ccggtcagtt gtttgccgac
      601 ggtcagcacc tgttcggcgg ggttgagttc gccggtttgc gcgttgcggc tgcgcttgct
      661 ggtaaagccc aaatcatcca ccagcccgat gcggtcgttg atttgcccgg caagcagcgc
      721 gccggcggct gcggacaggg cggcattgtc gccgctgctg ccactgccgg cgcggttgag
      781 gatgagccag gagagcttgt ctttttcact catcggttcg tttgccgtca gcgtaatgcg
      841 cgggctgttg aggctgccca atatttccac gcccgcaccg acgggggaaa ggcggcgttc
      901 ggcgcggatg ttgaggttgg gatcgttgag cgggccgaca aaggagaccg tgcctttggt
      961 aatgtccaaa tcctgcccgt atgccttata acgccctttg atgacgcgga ccgtgcccac
     1021 gccccgtacg cttccgcccg attgggcggt cagggtcagt ttgccgccta tggtaacgtc
     1081 cgcgccgtag ccggcgaagc ggatgccgtc attgaggtct aaagtcaggt tcatattgac
     1141 ggggagcggt gccgccgcct cttttttgac ttcgcctaat acgacgacat cgtcgccgac
     1201 ggacggcatc gaggattttt gcgaaccgaa cagcccctga tccgttttaa tcatcccggt
     1261 aacggatatg cctttttgcg gcgaatagcg caggcgggtg ttgccggaaa ccgtcaggcg
     1321 gcggttgggg cgggacagga tgcggtattt gtcgaacacc gcgccgatat cgacatcggg
     1381 tccgctgttt tccataccga ccgtaccgga gagttccgcc gtcccttcgt gccggaattt
     1441 caggctgtcg attacccatt tcctgcccgc gatatgcgaa cgcagcgagc cgttgtccaa
     1501 gatgatgcct tgggtttggt tgcggtaata gagtttgtcg ccgttgatgc tgccgcccaa
     1561 gtgcggatcg gcgatgctgc cgccgagggt tacggcggca ttcaggctgc ctttgacggt
     1621 ttgtccgacc ggtaggaagt tgcggaatac ttcggcatcg gcaacggtca ggttgagcct
     1681 gccgcccaaa ggcgcggtat cgaaagagcg gctttgcccg acggtgatgt tgccgttgat
     1741 tttcccgtag ttgctgctgc cgttgacggc ggcattgacg gacggagagc ctacccgtcc
     1801 gccgatttgc gcggcggcat tcaggctgcc ggtaatgttt tgcgcggcgg cgggcagaaa
     1861 gggcttcaat gcgcccaagt cgggaaggga ggcggtaatc ctgccgccga gcggtgcatt
     1921 tgccatattg ccgccgaagg cgttggcgat gcccaaatcg gcgttaatcc gcccgaaacg
     1981 cgcgccgccg tcaagcagga ttccgatgcg gtcgttttga aagcgcgttt tcagggaaaa
     2041 tgcgttcaaa cccaaagcct gcccgccggg caatacggca tcgccgcttt gccggctgat
     2101 attgaggtag ccgcgcgcgt tgcgcccgta ggcgacatcc cagtcgccgt ttaaaaccag
     2161 attgtgttcg aagggcggtt tgaagaaatt gtgcaactcg gcgatatgca gaccgtgtgc
     2221 gccgcctttt gccgatatgc cggttttttt atcccaagaa aagtgttgca ggttgaggct
     2281 gccgcccatt gcctgccaat ttgccgcact tgccgccacg cgttccgcac cggcttcgag
     2341 cgtcatacgg ttttgcagct tgaggttgaa tgcgccgccg atgtcgagga tgccgatgct
     2401 gcctttccat cgggtaagtt ccctgttgat gccgcctgaa gcgtccaaat cgaatttgaa
     2461 cggtttgcca tccagcgtca tggcggcgtg tgtgcggatg cggtgctgca cgcccgtgcc
     2521 gtccagcatc aggtcggcgg tatcgacaac cgccgctccg cccgacagcg aaaggcggct
     2581 gcctttgatg tcggcgcgta tcgggcggct tgtgtcgggc gaacctttga gcgtgaaatc
     2641 gagcgaacgg atgtctgccg ccttgccgat gtgcaggttg cgcgccgcgc cggaaaggtc
     2701 ggtttcaaag gttcggatgc cgccgtccaa atcgccggaa aggtgtccgc gtacatttaa
     2761 agaccccgcg agtccgaaac cgaaacggga taaatcgggt gcggtgatgt tgaggttgag
     2821 ccggtcgcct tttttgccga agccgccgtc tgttttaata atgttccgcc ccagccgcaa
     2881 atcgacggcg gcacgcggaa ggtggcggga ctcgtaaaca atgtcggcac tgccggcaat
     2941 cggtacgccg ttgaacgtgc cgggtaaaaa ccgcattttg cctgtgaatt tctcttttgc
     3001 cagttcgccg gcaaggttta ttgagccgtt gatattgcct gccggaagtt gcggattgat
     3061 gcgcgaaggg tcgaatgcgc gggaacggat gtccagcttg agcaggcggt ctttaaacag
     3121 ctcgagatag ccttgcgcgg tcaggctgcc ttgcccggcg gcgatgttga cggtgtcgag
     3181 caccagtttc cgctgtccgt ttgcggggtc gcttgcaatg gcgaggctgc cgtccgtgcg
     3241 cgccgtgccg atgccgagtt gccaagagat tttgggcgag gcggtcgtgc caccgatgcc
     3301 gatgctgccg tccaacctgc ctttgaacgc ggtttgcagt acgtcttccg cgccgacgga
     3361 gttgatgcct atatttaaat cgaggatgtc tttttcggtg tcgattttgc ccgacagcct
     3421 gatgccgccc cgtccgagca gggcggcgga cgtattgccg atatgcaccg tgccgtcctg
     3481 ccggatgaca aagccgccta aaacctgacg gacggggatg ccgttgcggt cggcaaagcc
     3541 ggctttggtg ttttccaaat cgagcgaacc ttccagcgcg atgccgtctg aaaacgacgg
     3601 gatggcggtc aggtcgaaat tcagcccggc atcgggcagg gagggcacga aggcggccgg
     3661 gttgatgttg aaccctttga ccagcacttc ttccaatgtt ttatccaatg attcggcaaa
     3721 cgggtggatg acggattttc ccgagaggcg gatattgccg ccgtcgatcg ccagttcggc
     3781 gcgcacatcc ttcaggctgc cgctcagccg agccgtactg tgtatggttt tgccttcgag
     3841 tccgcctttg gtgtaaatgg cggtatcgag ggcaaacggt tttttcaagc cgaccgaggc
     3901 cgcccccgac gaactgctcc acggtgtgtc ggcggccttc aggtcaaggc ggtgtccttt
     3961 gcggtcgtaa cggtatgaag catccagccg ttcgagatag acggtttgtt tgtcaaaggc
     4021 tttgcccatg ctgattttgc ccgtctcgaa gcggtcgaga tagacggcgg caggcaggtc
     4081 tatgctgtcg ggaaggctga gcggcgggcg ttcttcttta ggcggagtcg gtttggtaac
     4141 gatggcgatg tcgccggcgg aaatttcggt aatgtgcagg ctgcggcgca tcagttcgga
     4201 cggtttccac gcgaagcgga agcggctgat tttaaggtct gccccctcgg tttctatcga
     4261 ccagttgtcg ccgtcgaagc cgtcgagcag cgtgcctttg aggttttggg aggaaatgtt
     4321 tacgccgaac caagacggga tttggtacag cccgaagcgc aaacccgctt ccgtgccggc
     4381 gagccagccg aggaaacata ctgccaaaat cagaacagac agcagtgccg ccgacagctt
     4441 cagcagcggg cggcgttttt ttgccggcgg ggtagggcgg tgttcagacg gcattttgcg
     4501 cgtgccgttt tcggtcggat cggtatctgt cggtgcggta tcggtcataa tgtctgtttc
     4561 aaaatggttt gctgcaatgc cgtctgaagc ggccgtatcg gtttagaagc gtgttcccaa
     4621 gctgatgtgc cagcggattt tcttatcgct gtgcccgtag gcgatgtcga aggaaaacgg
     4681 cgcaagcggg ctgaaccagc gcacgcccag tcccgaaccg tgtttcagct tcatacgttt
     4741 gaaattggcg gcggcatcgc ccatatcgtg gaataccgcg ccggaaaggg tgcgcgtaaa
     4801 cggcagttgg tattccaggc tgcccaccaa gagggcgcgt tcgggcagga ccgatccgtt
     4861 cgggccggca agtccgatgc tgtcgagttc gtaacagcgc acggaagacg cgccgccgct
     4921 gcggaacatc agccccgagg ggacatcggc attgtcgcgg gcaagggtgt aacccgcttg
     4981 tccgcgtatg atgaacgtgc cgagtttctt gttttcgggc gtgaagaaat aacctgcgcg
     5041 ggcagaggtg cggatcagcg cggtggagga caggaatgtg cccaaagtcg taccgatttt
     5101 gccgtcgagg taatggccgt tttcgggatg cagcacgttg ttgagcagct ggcgtttcca
     5161 agaggcggtc agcatcgtgg cgtggctgtt gcccaaatcg atatccgagc cggggatttt
     5221 ccggccttct gcgagaaact ctgcccccag cctggcatcg atgcccgcgc ggtcgcgcac
     5281 ataccagatg ccgccggaga aggcgcgttt ttcgaggttt tgggtggtcg aacggttgta
     5341 ggaaacgttg cttgtccagt agttgccccg atagttgcgc ggctggctga tgccggcggc
     5401 aagcgtggtt tcgtatttgt ccatatccca gacgaccgag ccgatatagc ctttgttgaa
     5461 gaggttgtaa tagtcgtagg cgattttgcc gcccaaaccg tattccgaat cgaggcggat
     5521 gccggtttcg agtttgtggc gtttgacctc ggttacgctg actttgacgg gaacgcggtc
     5581 gccttggaga cggtcgaagt cggcttgtac ggacgcgccg gaataatgcc cgttttgttc
     5641 gagcgcctgt tggaagtcga gcagcaggtc gaggtcgtag ggcgtgcccg gttggaagcg
     5701 cgccaggccg gagacgattt gttcggggta acgctgcgtg ccggtgattt caaagtcgcc
     5761 gaaggcgatg gggcggccgc tgtccacgac gacattcaaa tcggcggtgg cggtatcggg
     5821 gttgacggcc gcccgggtgt tgccgagttt ggcaagcggg tagcctttgc gcgttaccgc
     5881 gccgaggacg gaagttttgc tgttttccca actgtcctga tcgaaatcgc tgcctaccgg
     5941 ctgctgccag ttttccagcg cgttgcggta gtattcggcg aggttgccgt ctgaaaggat
     6001 gtcgccgagg atggcgacgc cgacgttggc gattttggtg cgcgggcccg gtgtgatgtg
     6061 caccgtataa gctccgtctt tttccgtcag gctgaccttg ctgctgaaat agcctttgct
     6121 gcggagcatt gttttaacgt tgtccggcgc ttcttcggcg aggaagcccg tctgttcctt
     6181 gtccaatact tcttcctgct gctgcgtgat gagcggcagg tgttcttcga ccatatcttt
     6241 gatttcactg tcctgcgtgt cgatgcggac gggaaatttg ggttttaatt tgactgattc
     6301 ggtgttgggg cttttgtttt tgaacaatgc gaaacccgcc gccttgtttt cggaaaggtc
     6361 ggcggcagac gcgtatgcgt gcggaaagaa aaataaagcc ggcaggagca gggcggtcgg
     6421 tttgatcatc atggtacggg tgtcgggtat cggtgtggcg gctttcgatt ttaacactgt
     6481 tcggacgggg cgggacgctt cagacggcat accgccgccg gtatttcgga ggttgcggcc
     6541 cacctggaat gtatctgctt gttttaaggt attttgtttc caatccgcat atggaaaaca
     6601 ggctgcgttt cggaatagtg tcgaaaaata aacgggatta aataaattta ttttcgttca
     6661 ataaattaaa ccgttgcgcc gaatgataaa gcaaagcctg tccggcactg catagggtaa
     6721 atcaacgcaa ccttaaaaca cagccgaaaa ataaaaaaaa cacagagaat gagttttctt
     6781 ttaaaataca acatttttta acatatcaca acataaggaa gaattatggc gttcggcaaa
     6841 tccttatttc atgcgatcgg ccgcgtcagc ctggtcaggc agattgccgc cggtttggcg
     6901 ttgggtatcg taatcggttc ggtttccccg caactgggct tggcggcagg cttgttcggc
     6961 agcctgtttg tcggcgcact caaagcggtc gcgccggttt tggtatttat tttggtggcg
     7021 gccacaatcg cgcagcacca aaaaggcaac aaggcgcata tcaggccgat tatcgtcctt
     7081 tacctcatcg gtacgttttc cgcagccctg accgccgtca tcgccggtat ggttttcccg
     7141 acgcacattg ttttggcggg cgcgggcgat gtgtccgccg cgccgccttc cggcattgtg
     7201 gaagtattga aatcgctgct gatgaacctg gtcgccaacc cgattaacgc gattgccaat
     7261 gccaactata tcggcatttt ggcttgggct ttggttttgg gcgcggcgtt gcggaatcac
     7321 ggttcggacg ttacgcggca ggtcgttgcc gatttggcgg aagcggtttc caccgtcgtg
     7381 aaatggatta tccgttttgc ccctttgggc attttcgggc tggtgtcgtc cacaatcgcg
     7441 gagacgggtt tcggcgcgct ggcgggttat gcgaagctgc ttgcggtact gttgggctgt
     7501 atggcgttta tcgcgctggt ggtcaatccc gccatcgtgt ggtggaaaat ccgccgcaac
     7561 ccttatccgc tggtgttcac ctgcctgcgc gaaagcggcg tgtatgcctt ctttacccgt
     7621 tcttccgccg ccaatatccc cgtgaatatg gctttggcga aaaaactggg gctgcacgaa
     7681 gacacttatt cgatttccat cccgttgggg gcgaccgtca atatgggcgg cgcggcgatt
     7741 acgattaccg ttttggctat ggcggcggcg cacacccaag gcatacaggt tgattttgcc
     7801 accgcgctgc tgttgagcct ggtggcgacg gtcagcgcgt gcggcgcgtc cggcgtggcg
     7861 ggcggttcgc tgctgctgat tccgctggcg tgcagcctgt tcggcatcag caacgatgtc
     7921 gccatgcagg tggttgccgt cggctttatc atcggcgtga ttcaggattc ggcggaaacg
     7981 gcgttgaatt cttcaaccga tgttttgttt accgctgccg ccgatttggg ccgacagaga
     8041 aaccgggcgg aataaaccaa gctatgccgt ctgaagcccg tttggcgttc agacggcata
     8101 tttttacaat tttatgggca tatggcggaa acaataccgg ctgccttgaa tatgaagggg
     8161 atgatgatgt ttgaacgcag tgtgattgca atggcttgta tttttgccct ttcagcctgt
     8221 gggggcggcg gtggcggatc gcccgatgtt aaatcggcgg acacgctgtc aaaaccggcc
     8281 gctcctgttg ttgctgaaaa agagacagag gtaaaagaag atgcgccaca ggcaggttct
     8341 caaggacagg gcgcgccatc cacacaaggc agccaagata tggcggcagt ttcggcagaa
     8401 aatacaggca atggcggtgc ggcaacaacg gacaaaccca aaaatgaaga cgagggaccg
     8461 caaaatgata tgccgcaaaa ttccgccgaa tccgcaaatc aaacagggaa caaccaaccc
     8521 gccgattctt cagattccgc ccccgcgtca aaccctgcac ctgcgaatgg cggtagcaat
     8581 tttggaaggg ttgatttggc taatggcgtt ttgattgatg ggccgtcgca aaatataacg
     8641 ttgacccact gtaaaggcga ttcttgtaat ggtgataatt tattggatga agaagcaccg
     8701 tcaaaatcag aatttgaaaa tttaaatgag tctgaacgaa ttgagaaata taagaaagat
     8761 gggaaaagcg ataaatttac taatttggtt gcgacagcag ttcaagctaa tggaactaac
     8821 aaatatgtca tcatttataa agacaagtcc gcttcatctt catctgcgcg attcaggcgt
     8881 tctgcacggt cgaggaggtc gcttcctgcc gagatgccgc taatccccgt caatcaggcg
     8941 gatacgctga ttgtcgatgg ggaagcggtc agcctgacgg ggcattccgg caatatcttc
     9001 gcgcccgaag ggaattaccg gtatctgact tacggggcgg aaaaattgcc cggcggatcg
     9061 tatgccctcc gtgttcaagg cgaaccttca aaaggcgaaa tgctcgcggg cacggcagtg
     9121 tacaacggcg aagtgctgca ttttcatacg gaaaacggcc gtccgtcccc gtccagaggc
     9181 aggtttgccg caaaagtcga tttcggcagc aaatctgtgg acggcattat cgacagcggc
     9241 gatggtttgc atatgggtac gcaaaaattc aaagccgcca tcgatggaaa cggctttaag
     9301 gggacttgga cggaaaatgg cggcggggat gtttccggaa agttttacgg cccggccggc
     9361 gaggaagtgg cgggaaaata cagctatcgc ccaacagatg cggaaaaggg cggattcggc
     9421 gtgtttgccg gcaaaaaaga gcaggattga tgccgatgcc gcctgaagcg ggggcgttca
     9481 gacggcattg tgatgtttgg ggtggggtcc ggttcaagtc cgcatcagtt gataaacccg
     9541 tcgtctttca ttttcttttc ggaaaaatac tggccgaata ccagcagggc agcgaggatg
     9601 atgacaaaca gtcccgccat agtcagagtg attttgaaaa acaattccgc tttgagaaaa
     9661 gaaaaccaga gttgcacaat cagcagcagc acccagccgg caaacagtcc gagacaggca
     9721 taaacgccga gcttaaacac gatcccgctc caaaatgacg acgtaggcct cgcgtttgaa
     9781 aaacagcagc atacggcgca aatctttttc catcgttacg a
//
LOCUS       DAWFJR010000072         9785 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWFJR010000072 DAWFJR010000000
VERSION     DAWFJR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9785
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.072"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(210..938)
                     /gene="rmpM"
                     /locus_tag="WGR04_001559"
     CDS             complement(210..938)
                     /gene="rmpM"
                     /locus_tag="WGR04_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ0795084.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1302..2252
                     /locus_tag="WGR04_001560"
     CDS             1302..2252
                     /locus_tag="WGR04_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ0795085.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2365..3099)
                     /gene="fnr"
                     /locus_tag="WGR04_001561"
     CDS             complement(2365..3099)
                     /gene="fnr"
                     /locus_tag="WGR04_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ0795086.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3302..4723
                     /gene="hemN"
                     /locus_tag="WGR04_001562"
     CDS             3302..4723
                     /gene="hemN"
                     /locus_tag="WGR04_001562"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ0795087.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTVAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(5016..6590)
                     /locus_tag="WGR04_001563"
     CDS             complement(5016..6590)
                     /locus_tag="WGR04_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ0795088.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            6915..8015
                     /locus_tag="WGR04_001564"
     CDS             6915..8015
                     /locus_tag="WGR04_001564"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ0795089.1"
                     /translation="METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGR
                     LRRKLLDLQDTGADELHRSRMLSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHSYSVQLADLPLLAERTQIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRE
                     TRAVLNIGGIANISVLPPDAPAFGFDTGPGNMLMDAWMQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAADARQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WMAACWVNRIPGSPHKATGASKPCILGAGYYY"
     gene            8134..9075
                     /gene="mafA"
                     /locus_tag="WGR04_001565"
     CDS             8134..9075
                     /gene="mafA"
                     /locus_tag="WGR04_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0795090.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPA
                     YDTTATTKSDALSSVTTSTSVLNAPAAALTKNSGRKGERSAGLSVNGTGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGIVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            9079..>9785
                     /gene="mafB"
                     /locus_tag="WGR04_001566"
     CDS             9079..>9785
                     /gene="mafB"
                     /locus_tag="WGR04_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0795091.1"
                     /translation="MKPLRRLTNLLAACAVAAAALIQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQAAIQGNLGYTVRFSGHG
                     YEEHAPFDNHAADSASKEQGNVNEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKL
                     DRWGNSMEFINGVAAGAL"
BASE COUNT         2494 a         2903 c         2351 g         2037 t
ORIGIN      
        1 gcggcaatgg gacacaaacc tgaatctgaa tccgaacctg cacctcgatt ctgacgatag
       61 ccgtttcaga aaaccaccca aaaaaacaaa ccccgcaaaa gcgggggttt gtttcacatc
      121 atcgatgccg accgacgttt cacgaatatt gcaacagtac ctggtacaaa atccactaac
      181 ctcatgcatc ggcaagatat tgcctagcct tagtgttggt gatgattgtg tgccggcaca
      241 acctgacggg ttacgatgct acggattttc acatccacac ggcggtcagg ttcgatacat
      301 gcaatcagag cctcacgttt tttggcttta gagactttcg cacccagttt ggcaacttcg
      361 gcttcacaaa cttgagtcat ttgcgcttga gattcgccca agccgacagc agaaattcta
      421 gaaacaggta cgccgttgct gaccaggttg tttgccacta cgtatgcgcg gcgttcggac
      481 agggcctgat tgtatttgtc agaacccata aagtcggtat ggccttcgac gcggacagat
      541 tggatattgg tttgacccag gcgttgcgcc aatactttca ggttgtcttg agcttcggcg
      601 cgcaatgaat ccttatcgaa accgaacagg gttttggcag acagggaaat ggtttcatca
      661 acatattgcg gagcctgctc cacaacgacg acaggcgcgg gtgcgggttc gggttctggc
      721 tcgggttcgg gggcagcaac cgcatcgccg cattctacgc gaccttggct tgctttatca
      781 aagtaggcgt ttttccagca ttcgccatag ttgttgcgta cgatttcgtt cgactggccg
      841 cttacggtgt aaccctgaac ggacgcctcg cccgcaacag cagtgccgga agcgagcaat
      901 gcaacgaata atgcgcttaa tttcagctgt ttggtcattt tattccctca ttaaatttgt
      961 acagcagata cagaagcaac tgctgaattg ccaacataca ggccgctgta gaaacatggc
     1021 ggcaaatcgg atatttcagt ctccactata aagaagcagg gggcggactg tcaatactca
     1081 acgcctgaaa acaaccgtaa aacagcctgt ttcctgatag tctatcatgc cgaaaaatca
     1141 attccgtgtc attactttag ggggattttt tccatatcgc acgattgccg ttgcacaaaa
     1201 acaacaaaca caacggcaaa gccccatacc gtaccggcaa gaaaatatga taaattataa
     1261 acaatgttca gccacccgac acagacccac accgacccgc catgaaatta caacaattga
     1321 aatacgcctt agaagtttac cagcacaacc tgaacgtttc cgaagcggcc gaagccttgt
     1381 ttacttcgca acccggcatc tccaaacaaa tcaaattgct ggaagaagaa atcggcattc
     1441 agatttttat ccgcagcggc aagcgcgtgg tttcggtctc gcagccgggc aaggtggttt
     1501 tggatattgc ggaacgtatt ttacgcgatg tacagaacat taaaaatatc ggcagcgagt
     1561 ttaccggaca ggacagcggt tcgctgacgg ttgccacgac gcatactcag gcacgctatg
     1621 ccctaccctt gattgttgcc gattttgtga aacgctatcc gaaagtcaac ctgaccatca
     1681 aacaggggag ccctgccgcc atcgcacgta tggttacctc aggcgaggcg gacttggcga
     1741 ttgttacgga acggatagac gaccatcccg aactgggaaa acttccctgc tatgactgga
     1801 ctcatgcggt tatcgtaccg aacgaccacc ccttgctcga atgcagaaac cccctccgca
     1861 ttgaagattt ggcgaggttt ccgctgatta cttatgaatt tgcattcaat gcgggcagca
     1921 gcatcgcgcg ggcattttcc aaagcccgtt tggaacagcc ggatgtcgca ttggcggcgg
     1981 cggatacgga cgtattgaag acttatgtgc gcttgggttt gggcgtggga ctgatggcga
     2041 aaatggcgta caacccggat acggacggcg atttgcagct tgtggatgcg gcacacctgt
     2101 tcgagccgtc gccgacgtgg attgctttgc gcagcgatac ttatttgcgc ggatatgcct
     2161 acgactttat ccaagcgttt gcgccgcacc tgacacgcga gaaggtggat aggattctgt
     2221 acacgcccat cagcgaggat ttttcgattt aggcggctgc cggttttcag acggcacttt
     2281 gcggcagata caacaaacag gacagatgtt ttcgtctgcc ctgtgtttat tgagaatgcc
     2341 gtctgaaatg ttcgtacggg ttaatcaaat ggcgtgcgaa caaccggata ccatcttctt
     2401 caacacctgc agattgagga tcttgatgtg tttgtgttcg acggaaatca atccttcctg
     2461 atgaaattta gataatgtgc ggctgacggt ttcaagtttc agcccgagat aactgccgat
     2521 ttcttcgcgg gacattctta agatgaagtc gttggcagca aaacctcggg aataaaggcg
     2581 ttgggaaagg ttcaacagaa aggcggcaat ccgctcttcg gcgcgcatat tgcccaacag
     2641 cagcataaca ccttggtcgc gcacgatttc acgactcatc atgcggaaaa agtgggtacg
     2701 caggctgggg atattctgcc ccagttcttc gatgtgggta aacggcagtt cgcacacttc
     2761 gctgtcttcc aaggcgaccg cgtcgcaact gtgcacatgg gaacagatgc cgtccatgcc
     2821 gatgagttcg cccgacataa agaaacccgt tacctgatcg cggccgtcct gactggcgac
     2881 ggttgttttg aagaagcccg aacggatggc aaagagcgag gtaaaggctt cgccgacaca
     2941 gaacaggtat tcgccctttt tcaggcggcg gctttgacgg atgacggcat cgagttggct
     3001 gagctcgttg ggcagcagcc cgacaggcag gcagagttcc cgcaaagaac aggaagaaca
     3061 cagcgttttc atctgatgtg tagtattatg cgaagccata ccgtaccttt ttgtgcgctt
     3121 tgccccatca tgattatagt ggattaaatt taaaccagta cggcgttgcc tcgccttgtc
     3181 cttatttaaa tttaatccac tatatgtgct tattgacaca tatcaagaca ggtttatcat
     3241 actgtggcat tctaccaaac tatccaaaat ttgactaaca tcataaaatc gcacacacac
     3301 catgaaaatc attcagatac agaacaatca caatgtaaac gatgaccgcc ccgagtttga
     3361 ccgcgcgctg attgccagcc tgcccgccag cggcccgcgc tacacttcct accctaccgc
     3421 cgaccgtttc catgacggtt tccgcgaagg cgaatatatc aaagctttac atttgcgcgg
     3481 tatgggcgcg ttaaacaaac cgctttccct ttacattcac attccgttct gcaacaccat
     3541 ctgctactac tgcggctgca acaaaatcat caccaaagac aaaagccgcg ccgatgccta
     3601 catcgaatat cttgaaaaag aaatggaact gctcgctcca catctgaacg gacggcacca
     3661 gcttgcccaa ctgcacttcg gcggcggcac gccgaccttt ttgagcgacg aacagatcga
     3721 acgtgtcttc cgcatgatac gcaaacattt cgagttaatc cccaccggcg aatactccat
     3781 cgaaatcgac ccgcgcaaag tcagccgcga caccgtcctc atgctcggca ggctcggctt
     3841 caaccgcatg agcatcggca ttcaggattt cgaccccaaa gtgcaggcgg cggtcaaccg
     3901 catccaaagt tacgaagaaa ccaaagaagt catcgatgcg gcgcgcgaag cggggttcaa
     3961 atccgtcagc gtcgatttga tttacggcct gccgcaccag acttcggaaa gcatcaaaac
     4021 caccatcgat accgttttgt cgctcgatcc cgaccgcctc gccctttatc actacgccca
     4081 cctgccgcac gtgttcaaac cgcaacgccg catcgatacc gtcgccgttc ccgacagcga
     4141 agagaagctc gatatgctgc aatactgcgt ccaaaccctg acagagcgcg gctacgtctt
     4201 catcggcatg gatcatttcg ccaaacctga cgacgaactc tccatcgccc tcaaagaagg
     4261 cttcctccag cgcaacttcc aaggctattc gacctacgcg gattgcgatt tggtcgccat
     4321 cggcgtgtcg tccatcggca aaatcggcag cacctattcc caaaacgaac gcgacatcga
     4381 tgcctactat gccgccatcg acgaaggcag actgcccatc atgcgcggtt atcagctcaa
     4441 tcaggacgac atcctgcgcc gcaacatcat tcaggatttg atgtgccgtt tcgcgctcga
     4501 ctatcggatt tacgaaagta tgttcggcat cccgttcgac cgctacttca aagacgaact
     4561 ggcggatttg gaaaaactcg ccggcttggg attggtgcgc ctgaacagcc acgggctgac
     4621 cgttacccca aaaggacgct tcctcatccg caacatcgcc atggtcttcg attaccacct
     4681 gcgccataaa gaaaccaagg cgaaatattc gcaaacggtg tgattgcgtc taacgtacaa
     4741 atgccgtcgg caggatatca taacgttccg cacctttgtg tccgaccgtt ccgaaaccaa
     4801 gatatagtgg attaacaaaa accagtacag cgttgcctcg ccttgccgta ctatctgtac
     4861 tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc actataccaa acggcatatc
     4921 ccgacagaac agattgtgca taaggcacaa gcccgcacat tccatcaaca aaatgccgtc
     4981 tgaacacggg ttcagacggc atcagtattt tacaatcaga atactgcctg taaaaccaag
     5041 tagcagacaa ccgacaatac ggcggcggca ggcagggtaa tgacccacgc caaaccgatg
     5101 ggcttcatca gtttccagtt ggcattgcgg ttgaccagac cgatgccgag taccgcgccg
     5161 accaagatat gcgtactgga cacgggcagc cccatcagcg acgcgcccat cacgacggat
     5221 gcggcggaca gttcggcggt aaaacccgaa gcaggatgca tttccgccaa actcgtaccg
     5281 acggttttaa tcacctcttt accgacaaac cacaaaccga caatcagcgc gataccgaaa
     5341 gtcagcatcg caatcggggg gacgacattt tgcgcggcaa cgctgttggt acgcaaaaca
     5401 tccataatcg cggcaaacgg accgatggcg ttggcgatat cgttcgcccc gtggctgaat
     5461 gcgaagccgc aggcggtaaa gacctgcatc catgaaaaca tctgaaaggt tgatttgccc
     5521 aagtctttac gcttgaggct tttggcaaac acaaacgtcc ccatccacac cgccgcgcct
     5581 atcataaaga tggtcaggaa gctgttgacg ttgctcatcc ccaaatgcag gtttttcaag
     5641 cccttgaaaa tcagcatagc ggaaatcatc atcgcgccga acgaagcgat aaagggaatc
     5701 caagaatgca gcgctttgta ggaatcgaca ttgtttttac ggttgtcgaa cgcataaaga
     5761 ccgcggtaat actccgattg cagctcttgc ggatcgaatt cgggttcgtc gtaaatttgc
     5821 gcgtcgtgcg ccattttggt ggcgtactcg actttttcgg cttcggacaa accctcgaaa
     5881 aacaggcggt gccgttcttt ataggccttt ttttcctgct tgatgccctt gagcgtgcct
     5941 tccgcccaag cgttgtaatc taagacgttt ttcttgacgc gcgaaaacag gaaataggac
     6001 accgcgccgc ccaacacggg cgacaatacc caagaaacac caataccgcc cagcttgccc
     6061 caacgtatca aatcgcccga tgcggcatcg ttcattaccg ccatacatac cgcgctgccg
     6121 acaatgccgc cgataatgga atgggtggta gataccggaa gccctttttt cgaggcaaac
     6181 aacagccaca acgccgccgc caaaagcgcg gacatcataa taaacacaaa ctgtatgggt
     6241 tcgaaatcaa cacccttcaa atcgacgatg cctttgcgta tggtattggt tacctcaccg
     6301 cccgcgatga ccgcgccgct gacctcaaat accgccgcaa tcagcaaagc ctgcgggatg
     6361 gtcagcgtac ccgcaccgac gctggtgccg aaagaattgg caacatcgtt gccgccgatg
     6421 ttgaacgcca taaacacgcc gaacatcgtg gcaataatga ataaggcggc gtaattgtaa
     6481 tgggtataac ccaagcccca ataaataaaa tagccgactg tacctaccag catggcggca
     6541 aagacggcgt tgatggtttt aacatttgcg ctcatttgga tttgagccat ggcaatcttc
     6601 ctttaatgtg gtggaaatca tgagtgttgc cgacactgta ctgctgcctc cggtcgaact
     6661 gccgtttcgg gatatggcag acgcatcctt ccttctgaac cgcatccaaa tacccccaaa
     6721 cctgaccgaa tgcaaaactg ctgccggaaa cggtttgtat gacacttcta ctactggatg
     6781 ttgcgggcgc attataatat ttttccatcc gtcttgaaac atttatttac actttattta
     6841 cactgcggcg gcaaatcggt atacgagcgt caatacacgt taaaatggcg ttttgcacca
     6901 gtttgggagt gatgatggaa acacagcttt acatcggcat catgtcggga accagcatgg
     6961 acggggcgga tgccgtactg atacggatgg acggcggcaa atggctgggc gcggaagggc
     7021 acgcctttac cccctacccc ggcaggttac gccgcaaatt gctggatttg caggacacag
     7081 gcgcggacga actgcaccgc agcaggatgt tgtcgcaaga actcagccgc ctgtacgcgc
     7141 aaaccgccgc cgaactgctg tgcagtcaaa acctcgcgcc gtccgacatt accgccctcg
     7201 gctgccacgg gcaaaccgtc cgacacgcgc cggaacacag ttacagcgta cagcttgccg
     7261 atttgccgct gctggcggaa cggactcaga tttttaccgt cggcgacttc cgcagccgcg
     7321 accttgcggc cggcggacaa ggcgcgccgc tcgtccccgc ctttcacgaa gccctgttcc
     7381 gcgacgacag ggaaacacgc gcggtactga acatcggcgg gattgccaac atcagcgtac
     7441 tcccccccga cgcacccgcc ttcggcttcg acacaggacc gggcaatatg ctgatggacg
     7501 cgtggatgca ggcacactgg cagcttcctt acgacaaaaa cggtgcaaag gcggcacaag
     7561 gcaacatatt gccgcaactg ctcgacaggc tgctcgccca cccgtatttc gcacaacccc
     7621 accctaaaag cacggggcgc gaactgtttg ccctaaattg gctcgaaacc taccttgacg
     7681 gcggcgaaaa ccgatacgac gtattgcgga cgctttcccg attcaccgcg caaaccgttt
     7741 tcgacgccgt ctcacacgca gcggcagatg cccgtcaaat gtacatttgc ggcggcggca
     7801 tccgcaatcc tgttttaatg gcggatttgg cagaatgttt cggcacacgc gtttccctgc
     7861 acagcaccgc cgaactgaac ctcgatccgc aatgggtaga agccgccgcg ttcgcatgga
     7921 tggcggcgtg ttgggtcaac cgcattcccg gtagtccgca caaagcaacc ggcgcatcca
     7981 aaccgtgtat tctgggcgcg ggatattatt attgagcaca aatgccgtct gaaagaccgt
     8041 tccgccgttc agacggcatt ttcgcccctt ttgtttacaa acccttaaaa tccatttaca
     8101 ctcaaactcg ttcaaaacaa aacaaaccct gccatgaaaa ccctgctcct cctcatcccc
     8161 ctcgtcctaa ccgcctgcgg cacactgacc ggcatacccg cccacggcgg cggcaaacgc
     8221 tttgccgtcg aacaagaact cgtcgccgca tcgtcccgcg ccgccgtcaa agaaatggat
     8281 ttgtccgccc tgaaaggacg caaagccgcc ctttacgtct ccgttatggg cgaccaaggt
     8341 tcgggcaaca taagcggcgg acgctactct atcgacgcac tgatacgcgg cggctaccac
     8401 aacaaccccg aaagtgccac ccaatacagc taccccgcct acgacactac cgccaccacc
     8461 aaatccgacg cgctctccag cgttaccact tccacatcgg tcttaaacgc ccccgccgcc
     8521 gccctgacga aaaacagcgg acgcaaaggc gaacgctccg ccggactgtc cgtcaacggc
     8581 acgggcgact accgcaacga aaccctgctc gccaaccccc gcgacgtttc cttcctgacc
     8641 aacctcatcc aaaccgtctt ctacctgcgc ggcatcgaag tcgtaccgcc cgaatacgcc
     8701 gacaccgacg tattcgtaac cgtcgacgta ttcggcatcg tccgcagccg taccgaactg
     8761 cacctctaca acgccgaaac ccttaaagcc caaaccaagc tcgaatattt cgccgtcgac
     8821 cgcgacagcc ggaaactgct gattgcccct aaaaccgccg cctacgaatc ccaataccaa
     8881 gaacaatacg ccctctggat gggaccttac agcgtcggca aaaccgtcaa agcctcagac
     8941 cgcctgatgg tcgatttctc cgacatcacc ccctacggcg acacaaccgc ccaaaaccgt
     9001 cccgacttca aacaaaacaa cggtaaaaac cccgatgtcg gcaacgaagt catccgccgc
     9061 cgcaaaggag gataaaccgt gaaaccgctg cgcagactga caaacctcct tgccgcctgc
     9121 gccgtagcgg cggccgcact catacagccc gccctcgcgg cggacttggc gcaagacccg
     9181 ttcattaccg ataacgccca acggcagcac tacgaacccg gaggcaaata ccacctcttc
     9241 ggcgacccgc gcggcagcgt ttccgaccgc accggcaaaa tcaacgtcat ccaagactat
     9301 acccaccaga tgggcaacct gctcatccaa caggcggcaa tccaaggcaa tcttggttac
     9361 accgtccgct tttcaggaca cggatacgaa gaacacgccc ccttcgacaa ccatgccgcc
     9421 gacagcgcaa gcaaagaaca aggcaacgtc aacgaaggct ttaccgtata ccggctcaac
     9481 tgggaaggac acgaacatca tcccgccgat gcctacgacg gcccgaaggg cggcaattac
     9541 cccaaaccta cgggcgcacg agacgaatac acctatcacg tcaacggcac agcccgcagt
     9601 atcaaactca atccgaccga cacccgcagc atccggcaac gcatatccga caactacaac
     9661 aacctcggca gcaatttctc cgaccgcgcc gatgaagcca acagaaaaat gttcgagcac
     9721 aatgccaagc tcgaccgctg gggcaacagc atggagttta tcaacggcgt cgccgccggc
     9781 gcgct
//
LOCUS       DAWFJR010000073         9750 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWFJR010000073 DAWFJR010000000
VERSION     DAWFJR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9750
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.073"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(53..427)
                     /locus_tag="WGR04_001567"
     CDS             complement(53..427)
                     /locus_tag="WGR04_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795092.1"
                     /translation="MIIQNEFNLYPSNMLPERFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVTYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEEWLELAIKDTW"
     gene            complement(600..956)
                     /locus_tag="WGR04_001568"
     CDS             complement(600..956)
                     /locus_tag="WGR04_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ0795093.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITENQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(1099..1443)
                     /locus_tag="WGR04_001569"
     CDS             complement(1099..1443)
                     /locus_tag="WGR04_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795094.1"
                     /translation="MNLENYENILIKLLFYHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDLLPD
                     NMIINNFFEIND"
     gene            complement(1602..2003)
                     /locus_tag="WGR04_001570"
     CDS             complement(1602..2003)
                     /locus_tag="WGR04_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795095.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            complement(2007..2747)
                     /locus_tag="WGR04_001571"
                     /pseudo
     CDS             complement(2007..2747)
                     /locus_tag="WGR04_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(2817..3134)
                     /locus_tag="WGR04_001572"
     CDS             complement(2817..3134)
                     /locus_tag="WGR04_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795096.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(3131..3733)
                     /locus_tag="WGR04_001573"
                     /pseudo
     CDS             complement(3131..3733)
                     /locus_tag="WGR04_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253822.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(4159..4503)
                     /locus_tag="WGR04_001574"
     CDS             complement(4159..4503)
                     /locus_tag="WGR04_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795097.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(4541..4864)
                     /locus_tag="WGR04_001575"
     CDS             complement(4541..4864)
                     /locus_tag="WGR04_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795098.1"
                     /translation="MFLDDVNIFLDDLNTNPITDEWYMSNFADKHIKILESYEAFDIL
                     KQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
     gene            complement(4867..5055)
                     /locus_tag="WGR04_001576"
     CDS             complement(4867..5055)
                     /locus_tag="WGR04_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ0795099.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENTRTGEKTDFKFKD"
     gene            complement(5125..5469)
                     /locus_tag="WGR04_001577"
                     /pseudo
     CDS             complement(5125..5469)
                     /locus_tag="WGR04_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(5553..5855)
                     /locus_tag="WGR04_001578"
     CDS             complement(5553..5855)
                     /locus_tag="WGR04_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795100.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPIAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSIDKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(5878..6147)
                     /locus_tag="WGR04_001579"
     CDS             complement(5878..6147)
                     /locus_tag="WGR04_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795101.1"
                     /translation="MMTESHGYLSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYCWLGFDELPQSEKIKFLSYLNILSIHKEIQDETVNRVYTD"
     gene            complement(6168..6374)
                     /locus_tag="WGR04_001580"
     CDS             complement(6168..6374)
                     /locus_tag="WGR04_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795102.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            complement(6340..7089)
                     /gene="mafB"
                     /locus_tag="WGR04_001581"
                     /pseudo
     CDS             complement(6340..7089)
                     /gene="mafB"
                     /locus_tag="WGR04_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(7195..7566)
                     /locus_tag="WGR04_001582"
     CDS             complement(7195..7566)
                     /locus_tag="WGR04_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0795103.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(7563..7814)
                     /locus_tag="WGR04_001583"
     CDS             complement(7563..7814)
                     /locus_tag="WGR04_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795104.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            complement(7946..8152)
                     /locus_tag="WGR04_001584"
     CDS             complement(7946..8152)
                     /locus_tag="WGR04_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795105.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(8162..8839)
                     /gene="mafB"
                     /locus_tag="WGR04_001585"
                     /pseudo
     CDS             complement(8162..8839)
                     /gene="mafB"
                     /locus_tag="WGR04_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(8923..9225)
                     /locus_tag="WGR04_001586"
     CDS             complement(8923..9225)
                     /locus_tag="WGR04_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795106.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPTAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSINKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(9241..>9750)
                     /gene="mafB"
                     /locus_tag="WGR04_001587"
     CDS             complement(9241..>9750)
                     /gene="mafB"
                     /locus_tag="WGR04_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ0795107.1"
                     /translation="SAKQWADAHPNITATAQTALSAAEAAGTVWRGKKVELNPTKWDW
                     VKNTGYKTPAARPMQTLDGEMAGGNKPLESKNTVTTNNFFENTGYTEKVLRQASNGDY
                     HGFPQSVDAFSENGTVIQIVGGDNIVRHKLYIPRSYKGKDGNFEYIREADGKINHRLF
                     APNQQLPEK"
BASE COUNT         2746 a         1900 c         1562 g         3542 t
ORIGIN      
        1 cgagtttatc ctagttatcc aggatggtcc catctctttt atagatagct gcttaccaag
       61 tatccttaat tgctaattca agccactctt caaaactatt aaaaaattca tggttttcta
      121 tattatctaa attaatgaca ataaccctag gatttcctgt tacatcatta ccatcaaaat
      181 aagcttccca ttctccatta ctagcgaatg ggataagatt taaatcaggc aggatagaat
      241 ttctaaatat ataagttact tctgcccctt ctgttccata attctcaaac caccagtgaa
      301 aattatgtgg ctgaatataa ggtattaaag atgtatcgtt agagatacga acatactttt
      361 caggataaca aaacctttca ggaagcatat tactaggata taaattaaat tcattttgaa
      421 taatcatttc gttaccttag gatcataaat actaccatta ggaaactggg tagaccaatt
      481 tggtggtgct agttcatttt caatttgttt tttaattcta aattgaatag cttctcccca
      541 tgtttgcggc atctaatgct gcttgctctg catcaatatt actaaaaatt ggattagtct
      601 caaaaatgaa aaattactgt tttttcattt tttatagcat attcaagtac atgaataata
      661 tttttcacta agggctctgt agaaaaatct gaattttgta atttttcttt tacagccaaa
      721 atactttcct tatctattag ccaatccccc tcacttggtc caaataacca attatcggta
      781 atttcagaaa caagtctcca taatccacga ttttgatatt caataatttg attttccgta
      841 atttctacaa ctgtttcaga ttgaatgttt ttataagccc aatcaatcat ttcagtatat
      901 tcactaattg aaacattgtc ttttaaacag aaaattaaca ggttgtgttc attcatttgt
      961 ctaagttcct gatatgagtt tgttgttgta aataatcttt tgcttgctta ccttgtaaat
     1021 taaaggatta accgcacctt ggatgatgcc gcgaatatca tccatacggt taccccacca
     1081 atttttattg gtgtatcgtt aatcatttat ttcaaaaaag ttatttatta tcatattatc
     1141 agggagcagg tcgttaaaaa aaaccccctc aaattccatt aaattagtat ttatccagca
     1201 cataaacgaa ccaaagcgtt ctttccctga gtgtaaatat ttaaaattaa actcaaaata
     1261 agtttttttg cttagaaaat ttaatggttt ttctatagtg aaaaaaaagc ccttactcgt
     1321 tttcgttcga gatactattt taaataaatt tttattttca ataaaataag aatattcatt
     1381 tactaagttg ttatgataaa aaagtaattt tattaaaatg ttttcataat tttctaggtt
     1441 catttctagc tcaatttaaa atgatttcat tgttgccgta ccaatgcgaa atttaccatc
     1501 aacaaaattt cccttcactg atacaggtac accatcaact tgggtagttt tattttcagg
     1561 ctgcctgaaa gaaagatagg caacttatta ctcatcttat gctagccctc aataattgaa
     1621 tacgttgcac tagaaccaat tatttctatg aatgtttgct caaacataaa aatatagtat
     1681 ttaaagaatt ctctattcaa aggaaaataa ctttctttca atatccattc cgcataacac
     1741 tcatcaagaa gttcaaaaaa agattgacca ccattttcat gaagcaaagc tctatctgca
     1801 tcttccgaaa ttaaatccat aaaagtatct ataaaatacg attcatcaac aaccctaaaa
     1861 tgaataacat cttcaataaa aatttttatg gttttatccg tatttctata accaactgct
     1921 ataacttcta ctttatcaga gtttattgct gcattatcaa aatagaaata gccagttggg
     1981 aaatctaagc tttttaattt cataatttat cgtgtatttc catatctgat ttttattttt
     2041 cggttattac taattgttat ttcaagtgtt ggttctccac cactactatc attacgagca
     2101 ttaaccgcac gcccatcagg taaattgccc gttatcgttc cattaggacg tgtttgtaca
     2161 tttcgaacat tcagactatt aaaatcattt agtgcgtcat tgtaatcccc cactttatca
     2221 tactgaacag cgggaccttt tgttttactc ctttgagtag tattgctcaa aatgtctttg
     2281 atttgtaaat cactgcgcgc cttaccggct tctatgcgag ctgccacact actcgggcta
     2341 taggggtcgc tatcaatcgc aggattacca ccgctggttt tttgtccgct agcctgtttc
     2401 ccaacctgct gtactgtatc gccaatttca tctaccgtac ctaacgcctt agtctgcata
     2461 tggcgaacag caggtttttt atagccggta tttttaaccc aatcccattt ggtcgggtta
     2521 agttctactt ttttacctct ccaaaccgta cctgcggcct ctgctacggc aagggcagtt
     2581 tgggctgttg ctgttatatt cggatgggca tcagcccatt gtctggcgga attgatgccg
     2641 tcttttaccg caaaagcact gtcctgtaat atgctcgcgg cagcaagttg tgcttccggg
     2701 cttaattttc ctaaattatt aataccttgt agagtctgct gcgcagagat gtagcaactg
     2761 tatttttacc ccgtcgggta aaaatacagt tgctacggct gctgggaaag cagttactat
     2821 tttcccagtg gctcaaaatt aattgtttca gcagatattt ttataataat ccctacatca
     2881 ataataattt cactataaaa ttggtattta tatagctcta tttcgggtat aaacaaggga
     2941 aacaagctat cttccttatc ctcataatct acgaaattat ttgtatctaa tttaaaattc
     3001 aaaatattac tgcatttgat atatccgaaa taattacctt caattgtatc tgtaaaagat
     3061 aatgtaaggg tatttccatt atcggaaaag attatttcat aaaggcttac attgtgaaaa
     3121 atattttttt tcatttgata aaatatccag aatgatgacg attatttggc aatggattgc
     3181 cttcataatt ttgtcgttgc ggatattttc cctgtacacc atcggttcgc ggcactcttt
     3241 gggttcttat aacctctcca tttggacgaa tatctatttt cacgccatca ggacctttcc
     3301 atgtttcata ttgagcctca tctgaaccag tacgtctaaa accttgttct tgcaatgctt
     3361 gacgtaattc atctcgcgta gttggtctga tagatttaac aactggcttg tttcctcctg
     3421 ccatctcacc gtccaacgtc tgcatagggc gggcagcagg tgttttatag ccgttatttt
     3481 taacccaatc ccatttagtc gggttaagtt ctactttttt acctctccaa accgtacctg
     3541 cggcctctgc tacggcaagg gcagtttggg cagtagctgt aatgttcgga tgggcatcag
     3601 cccattgtct ggcggaattg atgccgtctt ttaccgcaaa agcactgtcc tgtaataggc
     3661 tcgcggcagc aagttgtgct tccggactta attttcctaa atcattaata ccttgtaaag
     3721 tttgctgcgc agagatgtag gtcggatatt tgtatccgac aaaaatattt gacgcatcta
     3781 tcgtttccga aatgccgctg ttggaaaatg tcggattcga gaatccgacc tacggctatt
     3841 tcgtttaagt cataaatagc cattcatgtt ccttctgaat ttgcttttgc tatccttaga
     3901 accaatcata taaacattat catcattaat taatgttcta agtttatgaa gcggtcaaat
     3961 ctgtcaaact accagagatg aagcatatcc tcctggaagt aattctgttc gtgctgtttc
     4021 catatcttct ttgccatcat cagttacttc tctgtctaaa taaaatctta cgataagttt
     4081 ttgtctaatt tcatcaaatt ctgccgtaat taatcttata ttgatatgaa tacacggttc
     4141 atacctgcta ttagccagtc aggtgtagtt ggatggggat tcatttttaa taatatcttc
     4201 aagcaagtga aatgtaagaa aagcgtagat acttgaaggc cggctatctg attggcaaaa
     4261 agaaatttcg gagataaaga atttccgttt atatttttca taaattaaat caacaccttg
     4321 ttttacataa aaactaactt cctcaggaag gatatttata acattctctc cttttacttt
     4381 tatagttaat ggtttgattt ttatgttttc atgaatatct gataggatta tttctaaaaa
     4441 attattttta tttccagtca ttctggctat tttatgtata tttccattgt gttcgattat
     4501 cattttattt tttccaagga tttccttcta tattgatagt ttatctaaag atttcattta
     4561 aaatatcctg actaatttct tgtttatata attctacaat tttctgtttc tgtgtatttg
     4621 tataaaattt ttcgttggta tctgcttgat attttaattg tctcaatatt tccatgattt
     4681 catattctga tttttcatca tattcttcaa tcatgtaatc aacaaattgt tttagaatat
     4741 caaaggcttc gtaactttcc aaaattttaa tatgtttatc ggcaaaattg gacatatacc
     4801 attcgtcagt gattggatta gtatttaaat catctaaaaa aatatttaca tcatctaaaa
     4861 acatgactaa tccttaaact taaaatctgt tttctcaccg gttcttgtgt tttcaatgta
     4921 atgtatttca attttgttta cgttttgaac acgcttaacc caaccgtctt ttgctaacca
     4981 accattttta gtatcggaca taggaggtat tctcgcagga gttttgccct gtgggtttgc
     5041 cttaacttct tccattgcta attgttcctt taaattatta ggtattgtac gaccggttga
     5101 tttttcaaca gattgtgtcc ctatttttaa tgatttgttt cccccagcca tttccccgtc
     5161 tacagtctgc atagggcggg cagcaggttt tttatagctg gtatttttaa cccaatccca
     5221 tttagtcggg ttaagttcta cttttttacc gcgccaaacc gtacctgcgg cctctgctac
     5281 ggcaagggca gtttgggcag tagctgtaat gttcggatgg gcatcagccc attgtctggc
     5341 ggaattgatg ccgtctttta ccgcaaaagc actgtcctgt aataggctcg cggcagcaag
     5401 ttgtgcttcc ggacttaatt ttcctaaatc attaatacct tgtagagtct gctgagcggc
     5461 tgtatctgag ccggtcagtg taggttggat taaaagccag cattttcaac atcttgatgt
     5521 tttactataa atctattctt gtgcttcctt tgttagataa atttcaacac aaaagatacg
     5581 tatgcattct ctaattttat ttgagataaa taatgcattg ctttgtcgat actgttaaat
     5641 aaattacttc cctcgtaata ccaattatca tatgtttgat aaaaataatt atctttcttg
     5701 atataaatat ctccccccaa tacaagtatt ctctcattga taaataaatt taatatatct
     5761 attgcaatag ggacgggtaa tgcggcttct ctaattccaa ttcctatcaa agattcccct
     5821 tgtctcgaat aaatttcaaa aatttcattt aacatgttaa tctctatcta ctatttttca
     5881 atcggtataa accctattca cagtttcatc ttgtatttct ttatgaatac ttaatatatt
     5941 aagatagctt aggaatttta ttttctcaga ttgaggcaac tcatcaaaac caagccaaca
     6001 ataaaaattg ttatatgatt ttaaaaaatc aacaattttt atttttccta aaaccaaatc
     6061 ttcgactaac tgggttgcat aaataatatc atttatgatt ttaacaggta aattatttga
     6121 taggtaacca tgggattccg tcatcattga tttctctagg tgcttattta tctgattggt
     6181 aagccttttg cgtaagcttc tttcgcctcc gcaagtgtca tttcattagg gccaccatct
     6241 atattagttt ctcctgtatt ttgccactga caaacgtcac agatatcata gtccataact
     6301 tcagttccgc aaacaggaca atgtagccat atttctccgt caatttccca tgtctttttt
     6361 gaattctcca tcataatact tctttcctaa gtttggttta aacattgttt tgattttgaa
     6421 tgtttttgga ttggccaaaa catagatatt gttattgata tcatagcgca ctcgtctatg
     6481 ttccgtctga taacctaata tcttgtccga tgtctgtttt aataacaaat cagacgccat
     6541 tttttggtag tcttcaatgg ttatatcgcc aaattctttt ccatgagctt tgaaatgtcc
     6601 atttaaagat ttctcagtag gaaacttgga ctttgtccat ctgatgcggt cttgaattgt
     6661 tcctgtcgga gaaatagatg tatcaggttt aacatatgag gcaggtaagc cgatattgtt
     6721 atcagagtgt gtagggtgtt gctgcgtact tggttttggt ggcttattcc cacctgccat
     6781 ctccccatct aaagtctgca tagggcgggc agtaggtgtt ttatagccgg tatttttaac
     6841 ccaatcccat ttggtcgggt taagttctac ttttttaccg ccccaaaccg tacctgcggc
     6901 ctctgctgcg gaaagggcag tttgggctgt tgcagttata ttcggatggg catcagccca
     6961 ttgtctggcg gaattaatgc cgtcttttac cgcaaaagca ctgtcctgta ataggctcac
     7021 ggcggcaagt tgtgcttccg gacttaaatt tcctaaatca ttaatacctt gtagagtctg
     7081 ctgcgcagag atgtaggtcg gatatttgca tccgacaaaa atatttgacg catctatcgt
     7141 ttccgaaatg ccgctgttgg aaaatgtcgg attcgagaat ccgacctacg gctgctacca
     7201 accatcattt tcagcttctt ttagccaagt atcaaaattt tcaaaagtct cgtaatattt
     7261 tgtattatct aaattaacta ctataatttt aggattacct gatttatctg aaatatcaaa
     7321 acaggcagcc cactcttggt ttctagcaaa aggtaatagg tttggaatgc cagttatttc
     7381 aaaataaatg tcaattactt ctgatatatt gctttctgca ttttcaaacc accaaggaaa
     7441 agaatattgt tcatcgtagt taatggcatg agtggattga gctaatttta aataactttg
     7501 cggatattta aatcctttag gaagcattgc ttcaggatac ggaacaaaac tattttgttt
     7561 tatcatttcc taagtacctt tacatcataa atacttccgt taggaaatct tttgctccac
     7621 ccttctggaa ctcctttttg tgtttcttgc tttctaattc gtagctggat agcttctccc
     7681 caagtttgag gctcaatacc atcatgctta aacctgattc tctcttttga tgataatcgg
     7741 tcattaaaat tatgaacatt cgcttccact tttactcctg cttcacgtgc tgccataata
     7801 cgcgtgttat ccatactagt aggtgctcca tcaggcatat taacaacatc aaccctgtcg
     7861 ccaacccatg atttttttct ttcatttcgg ctacaataga atcatagttg taatttgcac
     7921 taggctctga caaaaatttg ggttgttaag ttgtcatttt tttataaatt ccagcaaact
     7981 gttgttgtaa attatctact tcatgttttc taacaccaga tacattttca aattctcttg
     8041 aatctacaca tacgcccgca tatatctcat tcaagatagc tcgtaaaaca aaaacttcat
     8101 cttgagttag tgaaatagtt gagttttgat tatttatgga tagtatattc atggcttttt
     8161 cctataaatc atcataatat tttttacctg atgttggtct aaatgctgta cctccatcgt
     8221 cagaattttt atctcggata actattgtcc ctgttttatc atcccaatac gcagttctac
     8281 cgcgaggtaa ctctttcata tttgttggat ggctaacaat attttcaata tgccgagcaa
     8341 aatctgctgg tgaattgata tttaaatccg taaattcttg ttgtcttatg acatgcttgt
     8401 tataagcatg cccactacta atttgttctc caattagttg cgcttgtaaa gacggttgtg
     8461 tggaagcatc tgctttgctg ttggacgtta tagatttagg cggtctattc ccaccggcca
     8521 tttccccatc caaagtatgc atggtgcgaa cagcaggtgt tttatagccg gtatttttaa
     8581 cccaatccca tttggtcggg ttaagttcta cttttttacc gccccaaacc gtagttgcgg
     8641 cctctgctac ggcaagggca gtttgggctg ttgcagttat attcggatgg gcatcagccc
     8701 attgtctggc ggaattaatg ccgtctttta ccgcaaaagc actgtcctgt aataggctcg
     8761 cggcagcaag ttgtgcttcc ggacttaaat ttcctaaatt attaatacct tgtagagtct
     8821 gctgcgcggc tgtatctgag ccggtcagtg taggttggat taaaacccag cattttcaac
     8881 atcttgatgt tttactataa atctattctt gtgcttcctt tgttagataa atttcaaaac
     8941 aaaagacacg tatgcattct ctaattttat ttgagataaa taatgcattg ctttgttgat
     9001 actgttaaat aaattacttc cctcgtaata ccaattatca tatgtttgat aaaaataatt
     9061 atctttcttg atataaatat ctccccccaa tacaagtatt ctttcattta taaataaatt
     9121 taatatatct attgcagtgg ggacgggtaa tgcggcttct ctaattccaa ttcctatcaa
     9181 agattccccc tgtctcgaat aaatttcaaa aatttcattt aacatattaa tctctatcta
     9241 ctatttttca ggaagttgtt ggttaggggc aaataatcta tgattaattt ttccatctgc
     9301 ttctctgata tattcaaaat ttccatcttt tcctttataa gatctaggga tataaagttt
     9361 atgccttaca atattatctc cgccaactat ttgtattact gttccatttt cagaaaaagc
     9421 atctactgat tgcggaaaac catgataatc accattcgat gcttgcctta atactttctc
     9481 tgtataaccg gtattttcaa aaaaattatt cgttgttaca gtattttttg attccagcgg
     9541 tttattccca ccagccatct ccccatctaa agtctgcata ggtcgggcag caggtgtttt
     9601 atagccggta tttttaaccc aatcccattt ggtcgggtta agttctactt ttttacctct
     9661 ccaaaccgta cctgcggcct ctgctgcgga aagggcagtt tgggctgttg cagttatatt
     9721 cggatgggca tcagcccatt gtttggcaga
//
LOCUS       DAWFJR010000074         9745 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWFJR010000074 DAWFJR010000000
VERSION     DAWFJR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9745
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.074"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            171..1355
                     /gene="coaBC"
                     /locus_tag="WGR04_001588"
     CDS             171..1355
                     /gene="coaBC"
                     /locus_tag="WGR04_001588"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ0795108.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAEDMHRAVHRLIDKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDEELSFPETSKDEAAMQIVERLAVYL
                     NK"
     gene            complement(1422..3578)
                     /locus_tag="WGR04_001589"
     CDS             complement(1422..3578)
                     /locus_tag="WGR04_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0795109.1"
                     /translation="MPVPQPTAPYDHLTAEARTLLFRTASYLKPEEQVELEKAVAYAF
                     HAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKMVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3671..3877)
                     /locus_tag="WGR04_001590"
     CDS             complement(3671..3877)
                     /locus_tag="WGR04_001590"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ0795110.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(3936..4553)
                     /gene="gmk"
                     /locus_tag="WGR04_001591"
     CDS             complement(3936..4553)
                     /gene="gmk"
                     /locus_tag="WGR04_001591"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ0795111.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSQEVIQRRLAKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4681..5280
                     /locus_tag="WGR04_001592"
     CDS             4681..5280
                     /locus_tag="WGR04_001592"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ0795112.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYHLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5326..6114)
                     /locus_tag="WGR04_001593"
     CDS             complement(5326..6114)
                     /locus_tag="WGR04_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ0795113.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPP
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAVKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            6439..7794
                     /gene="yegQ"
                     /locus_tag="WGR04_001594"
     CDS             6439..7794
                     /gene="yegQ"
                     /locus_tag="WGR04_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ0795114.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDILEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHHTQDYQNYLTGHSTAKQSQYVGHVTEIDENGWATVEVKNR
                     FAVGDSLEIIHPNGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPDMQGKEKALIARVLN
                     P"
     gene            7928..8404
                     /locus_tag="WGR04_001595"
     CDS             7928..8404
                     /locus_tag="WGR04_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HEZ0795115.1"
                     /translation="MLAACQSGSAPKNAHTASADTPAGESCKVIRDDTAGSGKNIIYR
                     CCNGQAAINAAIADGVLESEIPVSFGGGGVPIANGRNLVTRQAANSVGKSDSEACEHA
                     LINAARKFQQTAGKLGGRSVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
     gene            complement(9009..9395)
                     /locus_tag="WGR04_001596"
     CDS             complement(9009..9395)
                     /locus_tag="WGR04_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEZ0795116.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGISARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(9392..9640)
                     /locus_tag="WGR04_001597"
     CDS             complement(9392..9640)
                     /locus_tag="WGR04_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEZ0795117.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLIGIEKP
                     ARNAGRLKKFSHMENTDISRILEDDNETAALFFEESAL"
BASE COUNT         2500 a         2620 c         2229 g         2396 t
ORIGIN      
        1 ccgcctccaa caatcccttc ctccccctcc tctgactggt gcgcctttgt gaatatactg
       61 tctgaaactt agggcttcag acggcatttt gtatccaaac ggtaactaat gtatccgcac
      121 tttgttatag aatggctgct gttttttctt cgtaattaga aattgtcaaa atgggtaaac
      181 atattctttt aggtgtaacg ggcagtattg cggcgtataa gtcttgcgag ttggttcgtc
      241 tgctgaaaaa acaggggcat tcggttacgg tggttatgag ccgctcggca actgaatttg
      301 tttctccgct gacttttcag gctttaagcg gcaatcctgt cctgaccgac acgcacggcg
      361 gcaacggttc aaacggtatg gaacatatca acctgacccg gaatgcggat gtttttctga
      421 ttgcgccggc aagtatgaat accgtggcaa aaatctgtaa cggcgtggca gataacctac
      481 tgaccagtct ggcagccgca cggaaatgtc cgctcgccat cgctcccgcg atgaatgtgg
      541 aaatgtggct caaccctgcc aaccaacgga atatcgcaca actggtttca gacggcatta
      601 ctgtctatat gccgggcttg ggcgaacagg cttgcggaga aaatggcatg ggaaggatgc
      661 cggaacctgc cgaattgctg gatttgcttc cggacttgtg gacaccgaaa attttaaggg
      721 gcaagaaaat cttgattacc gcaggtgcga catttgaagc cattgaccct gtcaggggca
      781 tcacaaatac ctccagcgga aaaatggggg tagctttggc gcgggcgtgc cgtgccgccg
      841 gtgcggaagt cagcctgatt cacggacagc ttcaaaccgc gctgcctttc ggcatatccg
      901 atacggttca agccgtcagc gctgaagata tgcatcgcgc agtgcatcgt ttaatcgaca
      961 aacaagatgc ttttatttct gttgccgccg tctcagacta taaggttaaa aacaggagta
     1021 cgcaaaaatt caaaaaagat aaaaatgcca aaccattatc catcgaattg gatgagaacc
     1081 ccgatatttt ggcttctatt gcctcattgc cgaacccgcc gttctgcatc ggttttgccg
     1141 ctgaaacgga gaatgtaatg gcatatgcgc gagaaaaacg tattaagaaa aaaataccag
     1201 tgatcgttgc caatgatgtt tcaatcgcga tgggcaaacc gaccaaccgg attaccatta
     1261 tcggggacga cgaggaactg tcttttcccg aaacaagtaa agatgaagcg gcaatgcaga
     1321 ttgttgaaag gcttgccgta tatttgaaca aataagcaat tgaacggata aaccataaag
     1381 cgggttgcct gttaatcaaa aggcaacccg ttttacctgc ttcaacttct gatgactttg
     1441 cggatatatg gaatactatg cagattttga ataatctgat tcaattgatt cagattcttg
     1501 actttcaata agaatttgaa ttcgacaaaa ccttccgttc ccgactggga tttagacggt
     1561 gtttcgaccg actcaatgtc cgcaccggaa tcggaaatcg cttgcgccat taatgccagc
     1621 aagccgtggc tgtcttccga ttggacttga agcccgacac ggtagttctg cccgttcata
     1681 ttttcccagt ctgcatccag ctgctgttcg ggatcggact ttaacaacgt cgggcaggta
     1741 tccctatgga taatcatgcc ttttccctta accaacagca aacggatgga atcgccggga
     1801 accggatggc agcactctgc aaaatgaata tgcccgcttt cctgaccatc aactttaatg
     1861 gaactgagcc tgacctcgct gccgaaatgc tcccctgcca actcggcaat gtgcatggcg
     1921 acataaacag gcagggtatg ccccataccg acgttgtaca gcacttcctc aaatgaagtc
     1981 tgtttgtcgt tgagatcggc aaggtatttt tctttaatgc cgtctgaaag caggacatct
     2041 ttgggcagca aactggacag ggctttttgt aagaggctct ctcccaaaac gaccgcatcg
     2101 tgccggttaa ggtttttaat atattggcgt atggcactgc gcgccctgcc tgacacggcg
     2161 aaattcaacc atgcaggatt gggtttggca tgttcggatg taataatttc gacggaatcg
     2221 ccggttttga gcttcgtacg caacggcatc atgatattgt tgatacgtgc ggcaacggtt
     2281 ttgtgcccga tatcggtatg caccgcataa gcaaaatcga caggcgttgc ccctttgggc
     2341 aaagttagga tttttccttt tggcgtaagg atgtagattt cgttcggaaa caaatcgact
     2401 ttgacgtgtt cgagaaactc aatggcattg gcactgcttg cctgcaaatc taagatattt
     2461 ttcagccacc ggtttgtgtg aagcaccgcc tgatcgacca tcttagaata tgatttatag
     2521 ctccaatgtc cggcgattcc accttcggca acagcatcca tttccttggt acgtatctga
     2581 acttcaatcg gcaagccgta agggccgacc aaagtcgtat gcagactttg atacccgttg
     2641 cttttcggaa tggcgatata gtctttgaac cgcccgggct tgggctgata gagggtgtgc
     2701 aatgcgccga gtgcggcata acaggctgga atgctgttga caatgacgcg gaaaccgtaa
     2761 atatccataa cctcggcaaa gcgcagcttt ttcgccatca ttttctgatg gatgccgtac
     2821 aggttttttt ccctgccttt gattttggcc tctatattcg cgcctaccag ccgctggccg
     2881 aatgcgcgca agactttgcc gacaacgtcc tgccggttct tccggctctt gtccatcgct
     2941 ttttttaaag tctcgtagcg gttgggatgc aggttttgga acgataaatc ctgaagctct
     3001 tgatatgcgt tattcaaacc tatacggttg gcaatctgtg catagatttc aagggtttcc
     3061 cttgcaatcc ggcggcgttt gtccgggcgc atcgaaccga gtgtccgcat attatgcaga
     3121 cggtcggcaa gtttaacgac aatcacgcgc acatctttgg tcattgccaa aatcagtttg
     3181 cggaaactct ccgcctgatg ttccgcatga tcttcaaatt tgagtttttc gagtttggac
     3241 agaccgtcca ccatctcggc aatcgtattg ccgaacaccg ccgccatttc cccttttgtc
     3301 acgcccgtat cttccaatac gtcgtgcatc acgcctgcac acagaccctg tatgtccata
     3361 tgccaaaggg cgagctgcgt cgcaacggca attggatgcg taatgtatgg ttcgccactt
     3421 ttccgggttt gtccgtcatg agcgtgaaat gcataggcaa cggccttctc gagctcaacc
     3481 tgttcttcag gcttgagata ggaagcggta cggaaaagca aggtgcgtgc ttcggcagtt
     3541 aaatggtcgt aaggtgcggt gggttgaggg acaggcattt cagacggctt tcggaacata
     3601 ttttattttt cgtttcaaac cgtcggactg cacggcggca gaatgtgccg acgtgcgttt
     3661 ccggcagaat ttatttattg cgcgtcaaca gttccgtacc gatatgcccg gcggcgattt
     3721 cccttaaggc ggtaacggtc ggtttgttat tgcggacatc gtccacaagc ggcgtattgc
     3781 cattttcaag ctggcgggcc cgacgggccg ctaccaatgt caggtcaaaa tggttggaaa
     3841 tttttccggt acagtcttcg gtggtaatac gtgccatatt atttgctttc tttcaaaaat
     3901 atttaaaata ggaaactggg tattttcgcg gttttttagg aattttccaa caaatctgca
     3961 ataaatccca gttgccgcga ccttttcaga cggcaggcat tcacaatatg gcgcaaatcc
     4021 tcctccgccc gcgccaagtc gtcattgacc acgacaaagt cgaacaatac ggactgttcg
     4081 atttcatgcc ttgccttcgc aagcctcctt tggataactt cctgactgtc cgtcccgcgt
     4141 ccgttgagac gcgcggcaag cacatcgaaa gacggcggca ggataaagat gccgacagct
     4201 tccggcagcg cgtcgcgaac ctgcgccgcg ccctgaacgt cgatttccaa aatcacgtca
     4261 taacctgccg ccgccaacgc attcacgccc tccgcgcttg tgccgtaata gttgccgaat
     4321 acgtctgcgt attccaaaaa agcctcctgt gcgataagcg actcaaactc ttctttggaa
     4381 acaaagtgat aatgtacgcc gtttgcttcg ccttcacgcg gcgggcgcgt cgtgtgcgac
     4441 acggaaacgc gcaaaccgtt atggtttgcc aacagccgcg acaccagcgt ggttttgccc
     4501 gtgccggaag cggccgaaat gataaagatg ttgccttttc gataagcgga catatttttt
     4561 acctgtatat tttccagccg attgtatcac aatggacacc cagtttccta tttgccgatg
     4621 cccatatttt gccgctattg ttttgattga tttggcaagc ggcaggctga cggctacaat
     4681 atggcgttaa aaacatcaaa cttggaacac gcaatgctgg ttcatcccga agctatgagt
     4741 gtcggcgcgc ttgccgacaa aatccgcaaa atcgaaaact ggccgcaaaa aggcatctta
     4801 ttccacgaca tcacgcccgt cctgcaaagt gcggaatact tccgcctttt agttgattta
     4861 ttggtttacc gctatatgga tcagaaaatc gacatcgttg ccggtttgga cgcgcgcggc
     4921 ttcattatcg gcgcggcact cgcctaccat ctcaacgtcg gttttgtccc catccgcaaa
     4981 aaaggcaagc tgccttttga aaccgtatcg caaagctacg cgctcgaata cggggaagct
     5041 gcggtggaaa tccacaccga tgccgtcaaa cccggttcgc gcgtgctgct ggtcgatgat
     5101 ttggttgcca cgggcggcac aatgcttgcc gggctggaac tgatccgcaa actcggcgga
     5161 gaaattgtcg aagccgccgc cattttggaa tttaccgacc ttcaaggcgg caagaatatc
     5221 cgtgcaagcg gcgcgccctt atttaccctg cttcaaaacg aaggctgtat gaagggctga
     5281 aaaccgaccc tgccgtctga aaccggcagg gttgttatga tgcgttcaaa tcacgcccaa
     5341 atcttgcaag cccctcaaca cgccgtcttc atcaacgccg gggcaaacat atttcgccgc
     5401 ctctttcact gcctgttccc cgttacccat tgccacaccg aacccgactt cagacagcat
     5461 ttccacatcg ttcaaaccgt cgccgaacgc catcacgtcc gccatttcca aacccaatgc
     5521 ttcaaccacg cttctgatgc cgtctgtttt cgacattccc gcaggcagca gatcgaccgc
     5581 ttcctcgtgc cagcgcaccg ttttcaagcc ttcccgttcc acaatatccg accagggcgg
     5641 catttcgttt tcctccgcaa acaccagcat ctgatacacc ggtttgcttg aaaaataatc
     5701 cttatcggca aaaaaatcgc tggcgatatg ctgcaaggcg cggcacacgc attccgacag
     5761 cgcggacacg gcgatcccct ctccgccgac aaacgcataa tccatgccca agccatccaa
     5821 atgcgcgcaa accctgccca tcaaaccggc atccatcggt acttcgcgca cagtttttcc
     5881 gtgcagcagc gcaaactgtc cgtttatcgt taccacggca tccatgcccg tttccgccat
     5941 catgtccctg accttttcgg gaatcgtcgc caaagaccgc cccgttgcca acgccgtcaa
     6001 tatgcctttg ccgcgcaaag ccgccaccgc cgttttcacg gaaggacgca aggtatccgt
     6061 atatttgcgg tacagcgtat cgtcaatatc gaaaaacacg attttaggat tcatcacatt
     6121 ctctctcgca ttcaaactgc cgcattgtat cccaatcagg caaatacttg ataaatcctt
     6181 ataaatttcc cggcaaaatt gaccgcgaac acgaaaaggc ggataatccg cccatcctca
     6241 aacccttttc agacggcatt tgcagcaatg ccgtctgaaa catttttaca aagcacacaa
     6301 atcatgtttc aacacacagg acgacacata aagcgtcgcc ctatatgttg ccctgattcg
     6361 aaaggggtta cacccctccc aaataaagtc tgatcctgcc gccctaaagg gcggggtttc
     6421 aaccgaaaag gaaacacgat gaaagcaccc gaactcttat tgcccgccgg cggattggaa
     6481 agaatgcgcg ccgcctacga ttacggcgca gatgccgttt atgccggcag cccgcgttac
     6541 tccctgcgcg cccgcaacaa cgaatttgcc aaactcgaca tcctcgagca aggcattaaa
     6601 gaagcgcacg aacgcaacaa aaaattcttc cttaccgtca atacgctgcc gcacaattcc
     6661 aaactcaaaa cctttgtttc cgatatggag cccttgattg cgatgaagcc cgacgcgctg
     6721 attatggcgg atccgggttt gattatgacc gtgcgcgaaa aatggccgga aatgccgatc
     6781 cacttgtccg tacaggcaaa caccaccaac tactggggcg taaaattctg gcaaaacatc
     6841 ggtgtcgaac gcattattct gtcacgcgaa ttgagcatgg aagaaattgc cgaaatccgc
     6901 caagaatgcc ccgacatcga actcgaagtc ttcatccacg gcgcattgtg catcgcctac
     6961 tccggccgct gcctattgtc gggttatttc aaccaccgcg accccaacca aggcacttgc
     7021 accaactcct gccgttggga ctacaaagtc cacaacgcta cggaaagcga tgcgggcgat
     7081 gcccagcttc tgcaaggttt caactttgaa aaagcccaag aagaagccaa ccaaaacttt
     7141 gaaggcatca acggtcaaaa acgccatccc tacgccgaca aggttttcct gattgaagaa
     7201 tccaaccgtc cgggcgagat gatgccgatt atggaagacg aacacggcac ctacatcatg
     7261 aactccaaag accttcgtgg cattgaagtg gttgagaaac tcgccaaaat cggcgtggac
     7321 agcctcaaag tcgaaggccg caccaagtcg ctctattatg tcgcacgcgt cgcccaatcc
     7381 taccgcaaag cgattgacga tgccgtcgca ggccgtccgt ttgattacag cctgttgagc
     7441 gaactcgaag gccttgccaa ccgcggctac accagcggct tcctcgaacg ccaccacact
     7501 caggattatc aaaactacct gaccggccat tccaccgcca aacaaagcca atacgtcgga
     7561 cacgttaccg aaatcgatga aaacggctgg gcaacagtgg aagtcaaaaa ccgctttgcc
     7621 gtcggcgatt cgctcgaaat catccacccg aacggcaacc aaaccatcaa attggaacaa
     7681 atgacccgca aaggccaacc tgtcgatgtc gcacccggca acggcattca ggtcaaaatc
     7741 cccgatatgc agggcaaaga aaaagccctc atcgcacgcg tattgaaccc ctgagccaca
     7801 atgccgtccg aaccccgttt tcagacggca ttttgttgaa tcttacggta caatggcaat
     7861 gatttcccac agcaaacaag gacatccgac atgaaaaaaa atcctcatac tctccgccgc
     7921 cgccctgatg ctggcagcct gccaatccgg cagcgcacct aaaaacgcgc acacggcatc
     7981 cgccgacaca ccggccggag aatcatgcaa agtcatccgg gacgacaccg caggaagcgg
     8041 caaaaacatc atttaccgtt gctgcaacgg tcaggcggca ataaatgcag ccattgcaga
     8101 cggcgtattg gaatcagaga ttcccgtcag cttcggcggc gggggcgtac cgattgcaaa
     8161 cggcaggaat ctggttacac ggcaagcagc aaacagcgtt ggcaaatccg actccgaggc
     8221 ctgcgaacac gccctaatca atgccgcccg caaattccag cagaccgccg gcaaactcgg
     8281 cgggcgcagt gttaccggct tccacagcta cttcgacaag caaccgctgc aaggaggaca
     8341 atacgactgt caggcaggct cgttccacgt ccgcgtggtc atgcgcgcca atgtcgtccg
     8401 ttaacatatc ggcgaccaaa aaattaggct gtgttttagt aatctgttga tttcaattat
     8461 ttgcaaagga aaagacaatt attttttcgg ttaggaagag acctatccta ttgaatacat
     8521 ttaagttaaa tacgtctatc aacactatat tatagtggat taacaaaaat caggacaagg
     8581 cgacgaagtc gcagacagta cagatagtac ggaaccgatt cactcggtgc ttcagcacct
     8641 tagagaatcg ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttaatcca
     8701 ctataaaaca cagccatata tattaagtac ctgctcttta tgcgcttttt taggaaagga
     8761 agatcaaacg gttcaatagc gaagtcttgc ccaaatttga tggacaggat atagaataca
     8821 tgcgtgcata ccacaccctg ccaaagttgg gagatgcact taaggttgca cttgtggtcg
     8881 ccaactatgc ctatacacgc ggatttcccg cgtttatagg ccgcgacgga agtctggctt
     8941 ttaagcgaag acaataaccg aaaagaccgc ctcggcggtc tttcttgtgt acacagtttt
     9001 tatatttgtc agcttgatgc attgacaact ctaattccat attgcggaat atattcatcg
     9061 acagtcatca gttcaaagcc ttccgcttgg gtttgtgcaa tcaacatcct atcgaaaggg
     9121 tctttgtgta tttccggaag gcttccagcc tgttttgcat gaaacagacc tataggcagc
     9181 atttcaaaat cctcttcttg aagcacatca aaaaactctt ccggtaattt caacaacccc
     9241 ttgttctgct tgatggaaat ttcccaaata cttgctgcac tgacaaagat cgcatttctc
     9301 ggattttcta tcagtttgcg tgcagatatc cccagtttct tgtcatccaa caaccaccac
     9361 agcaacgcat gggtatcaag cagaatcttt ctcacagagc cgactcctca aaaaataaag
     9421 ctgccgtttc attgtcatcc tcaagaatac gtgaaatatc cgtattttcc atatgactga
     9481 attttttcaa ccttcctgca tttcgtgccg gtttttcaat accgattagt tggacgcaag
     9541 gcttacctgc cttcgcaata ataacaattt cccctgcttc tgctctttga atcaattgac
     9601 tcaaattggt ttttgcctga tgaatatttg cttgaaacat aacacttctc atgattagct
     9661 aacttaacta atatacatca ttaccaagat tttgggaatc tcattacata tatttgatta
     9721 tatccgccgt tttattcaca ccttg
//
LOCUS       DAWFJR010000075         9395 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWFJR010000075 DAWFJR010000000
VERSION     DAWFJR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9395
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.075"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..408
                     /gene="rfbC"
                     /locus_tag="WGR04_001598"
     CDS             <1..408
                     /gene="rfbC"
                     /locus_tag="WGR04_001598"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ0795118.1"
                     /translation="VQENHSKSGKGVLRGLHYQTENTQGKLVRVITGEVFDVAVDMRK
                     DSPTFGKWVGEILSAENKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYNPKAEHSLIW
                     NDPTVGIDWPLQGEPNLSPKDLAGKVLSEAVTF"
     gene            433..2547
                     /locus_tag="WGR04_001599"
     CDS             433..2547
                     /locus_tag="WGR04_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ0795119.1"
                     /translation="MFLFSDGLQSINNNNRRKRIVKNAYIPSRGIRKIPHLSTLLPEF
                     HICKDGKEAEAVVGWGLRPTTHKARAFAAEHQLPFIALEDGFLRSLGLGVAGYPPYSI
                     VYDDIGIYYDTTRPSRLEQLILAADTMPSETLAQAQQAMDFILQHHLSKYNHAPELSD
                     DHPLRSPSKPETVLIIDQTFGDMAIQYGGADASTFELMFQTALNENPQADIWVKTHPD
                     VLCGKKQGYLTQLAQQHRVHLLAEDINPISLLQNVDKVYCVTSQMGFEALLCGKPLTT
                     FGLPWYAGWGVSDDRHPKIDSLVQTQRRAPRDLLQLFAAAYLQYSRYLNPNTGEAGSL
                     FDVIDYLATGKRKNDKLRGELYCVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVK
                     LSDDARILAWGNGKEAIVRFAEQHHIPLLRIEDGFIRSVGLGSNLVPPLSLVTDDMGI
                     YFNAETPSRLEHILQNQNFDDQDFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTV
                     ILVPGQVEDDASIRYGSPQIYRNLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISP
                     EDAARYADQTAEQTDILTCLQYADEVHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLT
                     QDLLPIPRRSRRLELWQLIAGTLIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNN
                     GLHRGYFAKKLGKIKQLYRSFK"
     gene            2684..3943
                     /locus_tag="WGR04_001600"
     CDS             2684..3943
                     /locus_tag="WGR04_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HEZ0795120.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELQGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSDSSTPAT"
     gene            4310..5716
                     /locus_tag="WGR04_001601"
     CDS             4310..5716
                     /locus_tag="WGR04_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ0795121.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYTEAAFVLG
                     NHLLKNGHPEQALPWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEQAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAANKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKDHPEALKIMGD
                     LYRYGLGIKADNHIAQDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            5934..7148
                     /gene="gltS"
                     /locus_tag="WGR04_001602"
     CDS             5934..7148
                     /gene="gltS"
                     /locus_tag="WGR04_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ0795122.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            7247..8263
                     /locus_tag="WGR04_001603"
     CDS             7247..8263
                     /locus_tag="WGR04_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0795123.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSVALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDADVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            8274..8471
                     /locus_tag="WGR04_001604"
     CDS             8274..8471
                     /locus_tag="WGR04_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795124.1"
                     /translation="MNTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAV
                     RLGENGFTEEQMENIANLFGR"
     gene            8489..8638
                     /locus_tag="WGR04_001605"
     CDS             8489..8638
                     /locus_tag="WGR04_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795125.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVAYADVSTHRTIQKTS
                     PYVPS"
     gene            8994..9287
                     /locus_tag="WGR04_001606"
     CDS             8994..9287
                     /locus_tag="WGR04_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795126.1"
                     /translation="MKTNDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSVHN
                     RKADYAEKGKEQIALFPRGLNGITKLPAAVLLPERPYHFDMKEVLHIFSWIPR"
BASE COUNT         2420 a         2706 c         2193 g         2076 t
ORIGIN      
        1 gtgcaggaaa accactccaa atccggcaaa ggcgtattgc gcggcctgca ctaccaaacc
       61 gaaaacacac aaggcaaact cgtgcgcgtg attaccggcg aagtgttcga cgtggccgtc
      121 gatatgcgta aagactcccc cactttcggc aaatgggtag gcgaaattct gtccgcagaa
      181 aacaaacgcc aactgtgggt acccgaaggt ttcgctcacg gcttctatgt actgagcgat
      241 gaagccgagt tcgtctataa atgcacagac tattacaacc ccaaagccga acactcgctg
      301 atttggaatg atccgaccgt cggcatcgac tggccgttgc aaggcgagcc gaacctgtcg
      361 cctaaagact tggcaggcaa agtattgtct gaagcggtaa cgttttaaaa ataattcagg
      421 ccgtctgaaa gaatgttcct cttttcagac ggcctacaat ccattaataa caataatcga
      481 cgaaaacgca ttgtgaaaaa cgcctacatc ccctctcgcg gcatccgcaa aatcccccat
      541 ctctccaccc tattgcctga atttcatatc tgcaaagacg ggaaagaagc agaggctgtt
      601 gtcggctggg gtttgcgccc gacgacacac aaagcgcgtg cttttgccgc tgaacaccag
      661 cttcccttta ttgctttgga agacggcttt ttacgatcgc tcggactggg tgtcgccggt
      721 tatccgccct actctatcgt ctatgacgac atcggcatct actacgacac cacacgtcct
      781 tcgcgtttgg aacaactgat tcttgccgcc gataccatgc cgtctgaaac cttggctcag
      841 gcgcagcagg cgatggattt catcctgcaa caccacctgt ccaaatacaa ccacgcgccc
      901 gaactttcag acgaccatcc tttacgttcc ccatccaaac ccgaaaccgt cctcatcatc
      961 gaccaaacct tcggcgatat ggccatccaa tatggcggcg cagacgcctc tacgtttgaa
     1021 ctgatgtttc agacggcctt aaatgaaaac ccgcaagccg atatctgggt aaaaacccat
     1081 cccgatgttt tgtgcggcaa aaaacaaggc tatctgaccc aactggcgca gcaacaccgc
     1141 gtccatcttt tggcagaaga catcaatccg atttctttgt tgcaaaacgt tgataaagtt
     1201 tattgcgtta cctcgcaaat gggttttgag gcgcttttgt gcggcaaacc gctgaccact
     1261 ttcggcctgc cgtggtatgc cggatggggc gtaagcgacg accgccatcc aaaaatcgac
     1321 agccttgttc aaacccaacg ccgcgccccc cgcgacttgc tgcaactctt cgccgcagcc
     1381 tatctgcaat acagtcgcta cctcaatccc aataccggcg aagcgggcag cctctttgat
     1441 gtcatcgact atctggcgac gggcaaacgt aaaaacgaca aattgcgtgg cgagttgtat
     1501 tgcgtcggca tgtctttgtg gaaacgcgcg gttgccaaac cgttctttaa cgtaccctca
     1561 tgccgtctga aatttatctc ctccacacaa aaactggcta gggtcaaact gtccgacgat
     1621 gcacgcatcc tggcctgggg caacggtaaa gaggccatcg tccgctttgc cgaacaacac
     1681 cacatccccc tgctgcgcat agaagacggc tttatccgct cggtcggact cggctccaac
     1741 ttagtgccgc cgctgtcgct cgttaccgac gatatgggta tttacttcaa tgccgaaacc
     1801 ccgtcccgtc ttgaacacat cttgcaaaac caaaacttcg acgatcaaga ctttcagacg
     1861 gccttgaagc tgcaaaaaat gctgaccgaa aaccacatca gtaaatacaa cgtcggcagc
     1921 tcagacttca ccgccccgtc aaccgacaaa accgtgatcc tcgttcccgg ccaggttgaa
     1981 gatgatgcgt ctatccgcta cggttcgccc caaatctacc gcaatctgga tttgctccgt
     2041 accgtacgcg aacgaaaccc caatgcctat atcatctaca aaccgcatcc cgatgtagtc
     2101 agcggtaacc gcatcggcca tatttcccct gaagatgctg cacgatatgc cgaccaaacc
     2161 gccgagcaaa ccgacatcct gacctgtctc caatacgcag acgaagtaca taccatgact
     2221 tcgctgaccg gttttgaagc cttgttgcgc ggcaaaaaag tcagctgcta cggcctgcct
     2281 ttttacgcag gctggggact tacccaagat ctgctcccca ttccgcgccg tagccgcaga
     2341 cttgagcttt ggcagctgat tgccggcacg ctcatacact atcccgacta tatccatccc
     2401 gaaacccatc aggccatcaa tgcggaaacc gctgcccaaa tcctgatacg acaaaaaaat
     2461 atgcaaaaaa acaacaacgg attacatcgc gggtactttg ccaaaaaatt aggtaaaatc
     2521 aaacaactat atcgatcttt caaataaata ccatcaaagt taacgatgcg tcataaactt
     2581 gcctctattg cggcatcatt gcctttgcat cgttaattct cttggcgtat gcttgaaagt
     2641 tcaacctaaa actattacat aaaaaacaaa accacattgc aacatgaaac agaccgtcct
     2701 caaaaataac ctgcaaaacc tgcttgaaag cgcagaaaat atcctgctgc ttcaaggccc
     2761 tgtcggcgat ttttttctgc gccttgccga ctggctgact gcaaacggca aaaccgtaca
     2821 taaattcaac tttaatgcag gcgacgacta tttttatccg cccactcaag cgcataccgt
     2881 tgtttttaac gacaactacg atgcctttcc tgagtttttg caagaataca tcactcaaca
     2941 tcacatccag gccgttgtct gctttggcga cacacgccct tatcacgtca ttgcaaaacg
     3001 cattgcaaac gaaaaccaag ccagtttctg ggcgtttgaa gaaggctatt tccgccccta
     3061 ctacatcacc ttagaaaaag acggcgtcaa cgcattttcc ccgttgccgc gccgtgccga
     3121 cttttttctt gaacaattcc ctaagcttgc ccagcaagaa tataaagcgc caacgccggt
     3181 acacggcggt tttacgccca tggcaaaaaa cgctatccgt tactatatcg agttgttccg
     3241 caatccacgc aaataccccg actacatcca ccaccgcgca cccaatgccg gccattacct
     3301 caaaccgtgg tcgctctcca tcctcaagcg tttgaactac tatattgaag acatccaaat
     3361 cgccaaacgt gtggaagcag gcaaatacgg caagtttttt attgttccct tacaggtatt
     3421 caacgacagc caagtccgta tccattgcga ctttcccagc gtccgcagct tcctgctcca
     3481 tgttttgagt tcatttgccg agcacgcgcc tgccgatacc aacatcatca tcaagcatca
     3541 tccgatggac cgcggtttta tcgactactg gcgcgacatt aaacgcttta tcaaagaaca
     3601 ccccgaactc caaggccgtg tgatttatgt ccatgatgtc cccctgcccg ttttcctgcg
     3661 ccacggtctc ggcatggtta ccatcaacag caccagcggc ctgtccggcc tgattcacaa
     3721 tatgccagtt aaggttctcg gccgtgccta ttatgatatt cccggcatta ctgaccaaaa
     3781 taccttggca gaattttgga atcatccgac accgcctgac aaagagctgt tccatgccta
     3841 ccgaatgtac cacctcaacg tgacccaaat taacggcaac ttctacagtc aggtcttttt
     3901 tcctaacaaa aaaacctccg actcttccac tccagcaacc tgacttagca aaaataattc
     3961 aggccgtctg aaaacatttc agacggcctg aaacaatcaa taccttagct actgccatgt
     4021 aaataaaaca caaaaacctg catttatcat taacaataaa ttacaaaaac agtataatga
     4081 ccgagctgtc atgagcgcat accgactcaa cctgagccct ttgtaacaca caaaatatgg
     4141 atgtatccct aggcaaaaca atataacaag ccaaacatcc taaagataag ccggcaaggc
     4201 aacacactct acaaaaccgt gccaagcaat atttttacaa agccctcaac cggtatcgcc
     4261 gcccatatgc cgcagcatcc gtcttccact ttatatccgc ccgcaaacca tgaccgccgc
     4321 tcctgatatc ctctaccggc aagccgccgc ccttttggaa caatccaata ccgcccaagc
     4381 cctgcccctg ctgcaacagg ccgcagagca gggatatacg gaagctgctt ttgtattggg
     4441 caaccatctg ctgaaaaacg gtcatccgga gcaggcactt ccatggttgg aagccgccgc
     4501 ggcccaacgc catcccaaag cactcttctc cctgctgcaa caacgcgaac acaacggcac
     4561 cccgaccgga cagcttctca acgactatgc ctggctgggt gagcaggggc actcagaagc
     4621 ccaattaatc ctcatgcgtt accacgcgca acgcaacgat ccacaatcgc tctactgggc
     4681 ggaacttgct gccgcccgat atgccgcacc tgcgtattac catctggcac gccatcatca
     4741 acgccaaggc gacgttgaaa cagccatcga acaatacgaa aaagcggcag cactcggcgt
     4801 aactgccgcc tgctggcaac tcggtcaaat ctacttctac ggtacaggtg tcagccccaa
     4861 ccacgcacaa gccgaacact atctcgaaca agcggcgcaa gccggccaca tcgccgcaca
     4921 aaccctgctg gccgaccttc ttgccgccca acgcaaaccc gaagccttag aatggtatcg
     4981 tcgtgccgcc aataaggagc aagcggaagc acaagccaag ctggcccaat acgccctgac
     5041 cggcgaactt tccgaacgcg atccgttcca agcggcacga tatgccaaag ccgctgccga
     5101 gaaagaccat cctgaagccc tgaaaatcat gggcgacctc taccgctacg gtctcggtat
     5161 caaagccgac aaccatatcg cgcaagatta ctaccaccgt gccgccgcgc tgggttctgc
     5221 cgccgcagca caaaaactca tcagcgacgc cgcgctgtac catccgcaac aatacgaaca
     5281 aatcaaaact gccgccctgc aacaacaaca aaccgaaacc atctaccgtt tggcggaagc
     5341 acaagcctgc gccatcggcc gtcccgccga ctacaatgcc gcgcgaaaaa attacatgga
     5401 agctgccggg ttccaccata aaaacgcagc ggcagcctta ggccgcatct accattacgg
     5461 cctcggtacg gcgcaagatc ctcgggcggc tgcacactgg tacgccattg ctgccgaaca
     5521 aaaccaccct tccgcccaat accacctcgc ctgtttttac tatcacgggc aaggtgtcgg
     5581 ctgtcatgtt ccgaccgcct gctactggct gcaggccgcc atcggcaacg gccacacttc
     5641 ggccgaatca ttaatatccc tattagaaca atggcgacgc gaagcacacc atgccatcgg
     5701 acaaaaggcc gtctgaaaag atttacactc gcattttttg acaatcttta actattcccc
     5761 taatatttgc cagttatttt tcacggacac gccattgttt tcatttcttt ctgaaaacac
     5821 cttgtccgcg catcaatacc atgacactcg gcggataacg ccaagcgttg aaacacacta
     5881 catccggaac aaaaacggat gctcggaaaa atatttctag gaggtgaaac aacatggaat
     5941 gggaattcaa cagttattac acactgattg ccgccacgct cgtgttgctg gttggtaaat
     6001 ttctggttca aaaaatcaaa ttcttacgag acttcaatat tcccgagccg gtcgctggcg
     6061 gtttggttgc cgcgattatc ctgtttgcgc tgcacgaggc gtacggcgtg agcttcaaat
     6121 ttgagaaacc gctgcaaaat gcgtttatgc tgattttctt cacgtccatc ggcttgagcg
     6181 cggatttttc ccgtttgaag gcgggcggtt tgccgctggt ggtttttacc gcgattgtgg
     6241 gcggatttat cttggtgcaa aactttgtcg gggtcggact ggctacggct ttgggtttgg
     6301 acccgctcat cggtctgatt accggttcgg tgtcgctgac gggcggacac ggtacgtcag
     6361 gtgcgtgggg acctaatttt gaaacgcaat acggcttggt cggcgcaacc ggtttgggta
     6421 ttgcatcggc tactttcggg ctggtgttcg gcggcctgat cggtgggccg gttgcgcgcc
     6481 gcctgatcaa caaaatgggc cgcaaaccgg ttgaaaacaa aaaacaggat caggacgaca
     6541 acgcggacga cgtgttcgag caggcaaaac gcacccgcct gattacggcg gaatctgccg
     6601 ttgaaacgct tgccatgttt gccgcgtgtt tggcgtttgc cgagattatg gacggcttcg
     6661 acaaagaata cctgttcgac ctgcccaaat tcgtgtggtg tctgtttggc ggcgtggtca
     6721 tccgcaacat cctcactgcc gcattcaagg tcaatatgtt cgaccgcgcc atcgatgtgt
     6781 tcggcaatgc ttcgctttcg cttttcttgg caatggcgtt gctgaatttg aaactgtggg
     6841 agctgaccgg tttggcgggg cctgtaaccg tgattcttgc cgtacaaacc gtggtgatgg
     6901 ttttgtacgc gacttttgtt acctatgtct ttatggggcg cgactatgat gcggcagtat
     6961 tggctgccgg ccattgcggt ttcggcttgg gtgcaacgcc gacggcggtg gcaaatatgc
     7021 agtccgtcac gcatactttc ggcgcgtcgc ataaggcgtt tttgattgtg cctatggtcg
     7081 gcgcgttctt cgtcgatttg attaatgccg cgattctcac cggttttgtg aatttcttta
     7141 aaggctgatt ttccgccttt ccgacaaagc acctgcaagg tttaccgcct gcaggtgctt
     7201 ttgctatgat agccgctatc ggtctgcacc gtttggaagg aacatcatgt atcggaaact
     7261 cattgcgctg ccgtttgccc tgctgcttgc cgcttgcggc agggaagaac cgcccaaggc
     7321 attggaatgc gccaaccccg ccgtgttgca aggcatacgc ggcaatattc aggaaacgct
     7381 cacgcaggaa gcgcgttctt tcgcgcgcga agacggcagg cagtttgtcg atgccgacaa
     7441 aattatcgcc gccgcctacg gtttggcgtt ttctttggaa cacgcttcgg aaacgcagga
     7501 aggcgggcgc acgttctgta tcgccgattt gaacattacc gtgccgtctg aaacgcttgc
     7561 cgatgccaag gcaaacagcc ccctgctgta cggggaaacc gctttgtcgg atattgtgcg
     7621 gcagaagacg ggcggcaatg tcgagtttaa agacggcgta ttgacggcag ccgtccgctt
     7681 cctgcccgtc aaagacggtc agacggcatt tgtcgacaac acggtcggta tggcggcgca
     7741 aacgctgtct gtcgcgttgc tgccttacgg cgtgaagagc atcgtgatga tagacggcaa
     7801 ggcggtaaaa aaagaagacg cggtcaggat tttgagcgga aaagcccgtg aagaagaacc
     7861 gtccaaaccc acgcccgaag acattttgga acataatgcc gccggagggg atgcagacgt
     7921 accccaagcc gcagaaggcg cgcccgaacc ggaaatcctg catcctgacg acggcgagcg
     7981 tgccgatacc gttaccgtat cacggggcga agtggaagag gcgcgcgtac aaaaccagcg
     8041 tgcggaatcc gaaattacca aactttgggg aggactcgat accgacgtgc aaaaagagtt
     8101 ggtcggcgaa caacgcaagt gggcgcagga aaaaatcagc aactgccgac aagccgccgc
     8161 gcaggcagac cggcaggaat acgccgaata cctcaagctg caatgcgaca cgcggatgac
     8221 gcgcgaacgg atacagtatc ttcgcggcta ttccatcgat taggggcaaa ccgatgaata
     8281 ccgtcccaaa aagcaggatt cccgtcaaac cgctgcccga aaaaaccaca gacgaagcca
     8341 aagtcgaaaa atggcggcag ctcggtgcgg aacacggttt gtcgggcgaa tgggcagttg
     8401 ccgtcagatt gggcgaaaac ggttttaccg aagaacagat ggaaaatatc gccaacctgt
     8461 tcggcagata aagagaaaat tgacggaaat gccgtctgaa accctgttat cggtttcaga
     8521 cggcattttg accaatacgg tacgcaggcg caaaacagcc ggcttttcct gtgttgccta
     8581 tgctgatgtt tcaacacaca ggacgataca aaaaacgtcg ccctatgtgc cgtcctgatt
     8641 cggaagggtt acgctccttc caaatatagt ggattaacaa aaaccggtac ggcgttgtct
     8701 cgccttagct caaagagaac gattctctaa ggtgctgaag caccaagtga atcggttccg
     8761 tactatctgt actgtctgcg gcttcatcgc cttgtcctga tttttgttaa tccactatat
     8821 tgtccattcg gcagggaaag cctgaagaag aattttcccg caagatggcc ggctgcctaa
     8881 ccgacttgga aaactgtttg gaaaaatacc tcgaacagtt cgggccggta tcggaaagta
     8941 tcgaagcctg taccgtcaaa ctgcaggagt aaccgtcgtt cttcaacagg ctgatgaaaa
     9001 ccaacgacaa gctcaatagg cagattgacg tgttgcagaa acagtcggca gccatccaca
     9061 acgaagccta tatcgaaatg aacacgctgc tttaccgcca ccgcgaagtg gtttccgtcc
     9121 acaaccgcaa agcagattat gcggaaaagg gcaaagagca gattgcgctg ttcccgcgcg
     9181 gattgaacgg catcaccaag ctgcccgccg ccgtcctgtt gcccgagcgt ccctaccatt
     9241 ttgatatgaa ggaggttctg catatattct cctggatacc gaggtaggcg tatcggctgc
     9301 cggataaaaa tgcaccccgt cattcctgtc tgtacggggg tagcggcagt gttttcctgc
     9361 ttcgttttat agtggattaa caaaaaccag tacgg
//
LOCUS       DAWFJR010000076         9268 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWFJR010000076 DAWFJR010000000
VERSION     DAWFJR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9268
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.076"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            165..1508
                     /locus_tag="WGR04_001607"
     CDS             165..1508
                     /locus_tag="WGR04_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ0795127.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPVITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPVSGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAITQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1511..2743
                     /locus_tag="WGR04_001608"
     CDS             1511..2743
                     /locus_tag="WGR04_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ0795128.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2736..3512
                     /locus_tag="WGR04_001609"
     CDS             2736..3512
                     /locus_tag="WGR04_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ0795129.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            3512..4138
                     /locus_tag="WGR04_001610"
     CDS             3512..4138
                     /locus_tag="WGR04_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ0795130.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGIMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4142..4735
                     /gene="nqrE"
                     /locus_tag="WGR04_001611"
     CDS             4142..4735
                     /gene="nqrE"
                     /locus_tag="WGR04_001611"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ0795131.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4749..5966
                     /gene="nqrF"
                     /locus_tag="WGR04_001612"
     CDS             4749..5966
                     /gene="nqrF"
                     /locus_tag="WGR04_001612"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ0795132.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGSGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPEEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6118..7173
                     /locus_tag="WGR04_001613"
     CDS             6118..7173
                     /locus_tag="WGR04_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ0795133.1"
                     /translation="MPSETRLPNFIRTLIFALSFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPNSEISRFNQTTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTTMSSEFEKLLR"
     gene            7199..7414
                     /gene="nqrM"
                     /locus_tag="WGR04_001614"
     CDS             7199..7414
                     /gene="nqrM"
                     /locus_tag="WGR04_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ0795134.1"
                     /translation="MKTLLLTFSIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7538..8116)
                     /gene="grpE"
                     /locus_tag="WGR04_001615"
     CDS             complement(7538..8116)
                     /gene="grpE"
                     /locus_tag="WGR04_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ0795135.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            8300..9118
                     /gene="cysE"
                     /locus_tag="WGR04_001616"
     CDS             8300..9118
                     /gene="cysE"
                     /locus_tag="WGR04_001616"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ0795136.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
BASE COUNT         2237 a         2583 c         2297 g         2151 t
ORIGIN      
        1 aaacagcttc cgcaaggaaa aaagcagcaa atctgacaaa caggtacaat tcctgataga
       61 atctaacact ttgctacatt aatttattca atgcttttga tgccacgcga cacggtatca
      121 aaacacgccg tttttacctt aaccatcaca agtaatgcaa atatatgatt aaaatcaaaa
      181 aaggtctaaa cctgcccatc gcgggcagac cggagcaagt catttatgac gggcccgtca
      241 ttaccgaagt cgcgttgctt ggcgaagaat atgccggtat gcgcccctcg atgaaagtca
      301 aggaaggcga tgccgtcaaa aaaggccaag tgctgtttga agacaaaaag aatccgggcg
      361 tggtgtttac cgcgccggtt tcaggcaaaa tcgccgccat ccatcgcggc gaaaagcgcg
      421 tgcttcagtc ggttgtgatt gccgttgaag gcaacgacga aatcgagttc gaacgctacg
      481 cgcccgaagc gttggcaaac ttaagcggcg aagaagtgcg ccgcaatctg attcaatccg
      541 gtttgtggac tgcgctgcgt acccgtccgt tcagcaaaat ccctgccgtc gatgccgagc
      601 cgttcgccat cttcgtcaat gcgatggaca ccaatccgct ggcggcagac cctgtggttg
      661 tgatcaaaga agccgccgag gatttcagac gaggtttgct ggtattgagc cgtttgaccg
      721 agcgtaaaat ccatgtgtgt aaggcagctg gcgcagacgt gccgtctgaa aatgctgcca
      781 acatcgaaac acatgaattc ggcggcccgc atccggccgg tttgagtggc acgcacattc
      841 atttcattga gccggtcggt gcaaacaaaa ccgtttggac catcaattat caagatgtaa
      901 ttgccatcgg acgtttgttt gcaacaggcc gtctgaacac cgagcgcgtg attgctttgg
      961 gtggttctca agtcaacaaa ccacgcctct tgcgtaccgt tttgggtgcg aaagtatcgc
     1021 aaattactgc gggcgaattg gttgacgcag acaaccgcgt gatttccggt tcggtattga
     1081 acggcgcgat tacacaaggc gcgcacgatt atttgggacg ctaccacaat cagatttccg
     1141 ttatcgaaga aggccgcagc aaagagctgt tcggctgggt tgcgccgcag ccggacaaat
     1201 actccatcac gcgtacgacc ctcggccatt tcctgaaaaa caaactcttc aagttcacga
     1261 cagccgtcaa cggtggcgac cgcgccatgg tgccgattgg tacttacgag cgcgtaatgc
     1321 cgctagacat cctgcctacc ctgcttttgc gcgatttaat cgtcggcgat accgacagcg
     1381 cgcaggcatt gggttgcttg gaattggacg aagaagacct cgctttgtgc agcttcgtct
     1441 gcccgggcaa atacgaatac ggcccgctgt tgcgtaaggt gctggaaacc attgagaagg
     1501 aaggctgatt atgggcttga aacattttct ggaaaaaatc gaaccgcact tcctgccggg
     1561 cggcaaacat gaaaaatggt atgccctcta cgaagctgcg gcgacgattt tctatacatc
     1621 cggtgcggta acgcgcaaag cggcacacgt ccgcgatgcg ctcgactcca aacgcatgat
     1681 gattttggtg tggctggctt tgttccctgc catgttctac ggtatgtaca acgtcggcgc
     1741 acaggcattc ggtgcgttaa cgcccgattt gctgcaacaa agcatcgcca acgactggca
     1801 ttacgccctt gccaacgctt tgggcatcaa tatgtcgtct gaagcgggcg tgttgggcaa
     1861 aatgctgttc ggcgcgattt acttcctgcc gatttacgcg accgtattta ttgtcggcgg
     1921 tttctgggaa gttttgttcg ccaccgtgcg caaacatgaa atcaacgaag gtttctttgt
     1981 tacctcgatt ctgtttgcct taatcgttcc gcccacgctg ccgctgtggc aggcagcttt
     2041 gggtatttct ttcggcgtgg tggttgcgaa agaagtattc ggcggtacag gtaaaaactt
     2101 catgaaccct gcgctggcag gccgtgcctt cctgttcttc gcctaccctg ccaacttgag
     2161 cggcgatgcg gtttggacgg cggttgacgg ctattccggc gcaaccgcgc tggcgcaatg
     2221 ggcggcacac ggtgcagacg gcctgaaaaa cgccgtaacc ggtcaaacca tcacttggat
     2281 ggatgcgttt atcggcaaac tgcccggctc catcggcgaa gtatccactt tggcactctt
     2341 aatcggcggc gcgtttatcg tgtttgcccg catcgcttct tggcgcatta ttgccggcgt
     2401 gatgatcggt atgattgcca tgtcttcgct gttcaacttc atcggttcgg acaccaacgc
     2461 tatgtttgct atgccttggt actggcattt ggtcgtcggc ggcttcgcca tcggtatgct
     2521 gtttatggcg accgaccccg tttccgcttc ctttaccaat gtcggcaaat ggtggtacgg
     2581 cgcactgatc ggtgtgatgt gcgtattaat ccgcgtggtc aatccggctt accccgaagg
     2641 catgatgttg gcgattctgt ttgccaacct gtttgccccg attttcgact atttcgtcgc
     2701 acaagcgaac atcaaacgca gaaaggcgcg cagcaatggc taagaaattc gataaagaca
     2761 gcttcagcgg cacgctgatt gtcgtgttgg cggtcagcct gatttgctcg gtcatcgttg
     2821 ccggtgcggt cgtcggcttg aaacccatcc aagagaaaca aaaactccaa gacaaacaag
     2881 gctatatctt gagcgtagcc ggtttgatgg ataaggacac cgacatcggt aaaacctttg
     2941 ccgagcgtat cgagcaacgt gttgtcgatt tggcgaccgg cgaatacgtc gccgatgcgc
     3001 ctaaagactt cagcgcgcgc atcgcaggca aagaccctgc ccaaagcatc cgcatcaaac
     3061 ccgaagacga tttggcaggc atcaaaagcc gtgccaaata caccgaggtt tatttggtaa
     3121 aaggcgaaga cggcaaaatc gggcaaatca tcctgcctat gcacggtaac ggtttgtggt
     3181 cggtcatgta cggctttgtc gccatccagc ccgacggcaa caccatcaac ggcattacct
     3241 actacgaaca aggcgaaacc ccgggcttgg gcggcgaaat cggcaatccg ttgtggcagc
     3301 aaaaattcgt cggcaaaaaa ctgtttgacg gacaaggcaa actcgccctg cacgtcggca
     3361 aaggcgcggg ttcggacaaa gaacacggcg tagatgccct ctccggcgca tcgctgacat
     3421 ccaaaggcgt gcaaggttcg ttcgcctact ggttcggcga aaacggctat atcccctacc
     3481 tgaacaaatt gaaatcagca ggagcgcaat aatggctgat atgaaacgct tgaaacattt
     3541 gatgttttca ccctttatcg acaacaaccc gattgccttg caggttttgg gtatttgttc
     3601 ggctttggcg gttaccacca aacttcagac ggccatcgtg atgggtattt ccgtagcttt
     3661 ggtaaccggt ttttccagct tcttcatttc gctggtacgc aactacatcc ccaacagcat
     3721 ccgtattatc gtgcaaatgg cgattatcgc gtcgctggtt acgctggtcg accaactctt
     3781 gcaggcattt gcctacgaat tgtccaaaca gctttccgta ttcgtcggtc tgattattac
     3841 caactgtatc gtgatgggcc gcgccgaagc atttgcgatg aaagagccgc cgctggaaag
     3901 cctgatcgac ggcatcggca acggcgcggg ctacgggata atgctgcttg tcgtcgccac
     3961 cgtccgcgaa ctgattggct cgggcaaact cttgggctac accgttttcc aaaccgtgca
     4021 ggacggcggc tggtatcaga ccaacggctt gttcctgctc gcccccagcg cgttcttcat
     4081 catcggcttt ttgatttggg gactgcgtac ctggaaaccc gaacaggcgg aggaataagc
     4141 tatggaacac tatttgagcc tcttcatcaa atccgtcttc attgaaaata tggcactatc
     4201 cttctttttg ggtatgtgca cgtttttggc ggtatccaaa aaagtatcca ccgcattcgg
     4261 tttgggtgtg gcggtaattt tcgtactcgg gctgtccgtc cctgtcaacc aactcgttta
     4321 ctcgctgctc aaagacggcg cgattgtcga aggcgtggat ttgacctttt tgaaattcat
     4381 caccttcatc ggcgtgattg ccgctttggt gcagattttg gaaatgttct tggataaatt
     4441 cgtccccgcc ctctataacg cattgggcat ctacctgccg ctgattaccg tgaactgcgc
     4501 gattttcggc gccgtatcgt ttatggcgca acgcgaatac aacttcggcg agtccgttgt
     4561 atacggcttc ggcgcgggct tgggctggat gttggcgatt gtcgctttgg cgggcattac
     4621 cgaaaaaatg aaatattcgg acgctcccaa aggcctcaaa ggcttgggca ttacctttat
     4681 cgccgccggc ctgatggcga tggcgtttat gtcgttctct ggcatccagt tataagaaag
     4741 ggatcggtat ggagattatt ttaggtatcg tgatgtttac cgtcatcgtt ttggttttgg
     4801 cactgatgat tctgtttgcc aaatccaaac tggtcagcga aggcgacatc accatcaaag
     4861 tcaacggcga aaaagagctg acgatgcccg ccggcggcaa actcttgggc gcgcttgcca
     4921 acgaaggcat ctttatcccc tccgcctgcg gcgggggcgg ttcgtgcgga caatgccgcg
     4981 tcgtcgtaaa aagcggcagc ggcgacatcc tgccgaccga gctgtcccac atcagcaaac
     5041 gcgaagcacg cgaaggctgc cgtctgtcgt gccaagtcaa cgtcaaaacc gacatggaca
     5101 tcgaagtccc cgaagaagtg ttcggcgtga aaaaatggga atgcaccgtc atctccaacg
     5161 ataacaaagc cacgttcatt aaagaactca agcttgccat tcccgaaggc gaagaagtcc
     5221 ccttccgcgc cggcggctac attcaaattg aagccccgcc gcacaccgtt gcctacaaag
     5281 acttcgacat tcccgaggaa taccacgaag actgggacaa atacaatttg tggcaatacg
     5341 tttccaaagt ggacgagccg attttgcgtg cctactctat ggcttcgtat cctgaagaaa
     5401 aaggcatcat tatgctgaac gtgcgtatcg ccacgccgcc cccgcgcgta cctgatgcgc
     5461 ctccgggaca aatgtcgtcc tacatctggt cgctcaaacc cggcgacaaa gttacgattt
     5521 ccggcccgtt cggcgaattt ttcgccaaag acaccgatgc cgaaatggta tttatcggcg
     5581 gcggtgcggg tatggctccg atgcgttccc acattttcga ccagttgaaa cgtttgaact
     5641 ccaaacgtaa gattaccttc tggtacggcg cacgttccaa acgcgagatg ttctatgtcg
     5701 aagactttga ccaactcgcg gcagagttcc cgaacttcac gtggcacgtc gccctgtccg
     5761 acccattgcc tgaagacaac tgggacggct acacaggctt cattcacaac gtggtttacg
     5821 aaaaccatct gaaaaaccac gaagcgccgg aagactgcga attttatatg tgtggtcctc
     5881 cgatcatgaa ccagtccgtc atcaaaatgc ttaaagactt aggcgtggaa gacgaaaaca
     5941 tcctcttgga cgatttcggc ggttaaatat tcggcttcaa acacaaaagg aagaaacctc
     6001 ggtttcttcc tttttttatc cgataccgtc cgaacacctt tcagacggca tcggcataga
     6061 aacataccgg ccgttttgcg atataattca aaccatttgc caaaaccgtc aaacactatg
     6121 ccgtctgaaa cacgcctgcc gaactttatc cgcaccttga tatttgccct gagttttatc
     6181 ttcctgaacg cctgttcgga acaaaccgcg caaaccgtta ccctgcaagg tgaaacgatg
     6241 ggcacgacct ataccgtcaa atacctttca aacaatcggg acaaactccc ctcccctgcc
     6301 gaaatacaaa agcgcatcga tgacgcgctt aaagaagtca accggcagat gtccacctat
     6361 cagcctaatt ccgaaatcag ccggttcaac caaaccacgg ccggcaaacc cctccgcatt
     6421 tcaagcgact tcgcacacgt tactgccgaa gctgtccgcc tgaaccgcct gacacacggc
     6481 gcgctggacg taaccgtcgg ccctttggtc aacctttggg gattcgggcc cgacaaatcc
     6541 gttacccgtg aaccgtcgcc ggaacaaatc aaacaggcgg catcttatac gggcatagac
     6601 aaaatcattt tgaaacaagg caaagattac gcttccttga gcaaaaccca ccccaaggcc
     6661 tatttggatt tatcttcgat tgccaaaggc ttcggcgttg ataaagttgc gggcgaactg
     6721 gaaaaatacg gcattcaaaa ttatctggtc gaaatcggcg gcgagttgca cggcaaaggc
     6781 aaaaacgcgc gcggcgaacc gtggcgcatc ggtatcgagc agcccaatat cgtccaaggc
     6841 ggcaatacgc agattatcgt cccgctgaac aaccgttcgc ttgccacttc cggcgattac
     6901 cgtattttcc acgtcgataa aaacggcaaa cgcctctccc atatcatcaa cccgaacaac
     6961 aaacgaccca tcagccacaa cctcgcctcc atcagcgtgg tcgcagacag tgcgatgacg
     7021 gcggacggct tgtccacagg attattcgta ttgggagaaa ccgaagcctt aaagctggca
     7081 gagcgcgaaa aactcgctgt tttcctgatt gtcagggata aaggcggcta ccgcaccacc
     7141 atgtcttccg aatttgaaaa actgctccgc taaacataca aaccgattag gaaacaccat
     7201 gaaaaccctg ctccttacct tcagcatctt cctgaccgtc atcatcggta tggcggtcgg
     7261 ctatattttc tccaaacgca gcatcaaagg cagttgcggc ggcattactg ccttgggtat
     7321 gaaaaaaatg tgcgactgcg acacaccttg cgacaccctg caaaagaaac tggatgaaga
     7381 aaaacaggca ggcggcatac gggttgaccg ttaacgccga aacaaaatgc cgtctgaagc
     7441 ccgacaggcg ggttcagacg gcattgccgc tttacttcga tcggtttata cgcaaaacgc
     7501 gcttcaggaa ataaatcaga ttattcccca gacgccttca ggcttccttc tgcgccaccg
     7561 taaccatagc cgggcgcaac acgcggtcgg acagcgtata acccttcttc atcacaccca
     7621 ccacggtatt cggctcctgt tcgcttgcca ccgcctgcat cgcctgatgg atattcggat
     7681 cgagcttatc gcccgcttta gggttgattt ccttgatttg cgtagcatca aatgctttct
     7741 gcaactcgtt caaagtcatc tgcacgccca ttttcagcgc atcgaaattg ccgctctgat
     7801 ccaaaagcgc catttccaga taatccttga ccggcagcat ttccacggca aacttctgtc
     7861 cggcgaactt gtgcgtatcc gcaatttcct gctggtggcg gcggcgcagg ttttgctcgt
     7921 ttgccaaagc gcgcagctgc tcgtctttca actgcgcttc cagctcggca atccgcgcct
     7981 gcaaatcctc ataagccggc tctgcggcag cctgttcctg cacaccgtcc gcatttccta
     8041 ctgtctcgac ggtttccacc gcctccacat tttcaaccgc ttcttcactg ttttgctgct
     8101 gtgtctgttc gctcatatcg tatcccttaa aacaaattgg aaatcaaaat ccagttatta
     8161 cccgcgcaag ataaggacat tttcaagaaa cttcaagcaa aagcagagaa tttcaaattc
     8221 attttcaaat cccctaccga aaaaataata tagacggtaa gatactgccg accatatcga
     8281 ttttcaaacg tcatcactca tgaaaaaaga ccatttaaac acaaccggtt tcgacctctg
     8341 gcacaccatc cgcgaagaaa ccgcggccgc ggtctccgcc gaaccgatgc tggcaagttt
     8401 tttgcaccaa accgtgttgc gccacgagtc cctcggctcc gtccttgcct accacctttc
     8461 cagcaaactc ggcagcccga ttatggacgt gcgcgcgctg tttgaaatct accagcaggc
     8521 gttgggcagc gacacccaaa tcggcaaatg cgtcgaagca gacttaaaag ccatctacga
     8581 acgcgatccc gcctgcgacg aatattcgct gccgctttta tatttcaaag gcttccacgc
     8641 gattcaggca caccgcatca accatcggct gtatctcgac gggcgcaaaa cgctggcgta
     8701 tttcctgcaa aaccgtatgt ccgaagtatt cggcgtggac atccatcccg ccgcccgttt
     8761 cggatacggg ctgatgctcg accacgccac cggctttgtt gccggcgaaa ccgccgtgtt
     8821 gggcaacaat atttcgattt tgcacggcgt aacgctcggc ggttcgggca aagaaggcgg
     8881 cgaccgccac cctaaaatcg gcgacggcgt gatgatcggc gcaaacgcct cgatattggg
     8941 caatatccgc atcggcagca atgccaaaat cggggcgggc agcgtcgtgg tttcagacgt
     9001 gccgccgtcc atcacggttg tcggcgtacc cgccaaaccc gtggcgcgat cgctcaaaac
     9061 accgtcggcg gatatggatc aaaatatcca gtttaccgaa atcgacttta tgatttgaat
     9121 atccgcacca atgccgtctg aaacgccaaa cgggcttcag acgacattgc ccgcttcaag
     9181 cctgcgcctt gatatgccgc cgatgtcggc acttgccagt cggacaaaat gccttttatt
     9241 caatacaccc gattcccacg caaccgga
//
LOCUS       DAWFJR010000077         9205 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWFJR010000077 DAWFJR010000000
VERSION     DAWFJR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9205
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.077"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(134..1576)
                     /gene="aldA"
                     /locus_tag="WGR04_001617"
     CDS             complement(134..1576)
                     /gene="aldA"
                     /locus_tag="WGR04_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ0795137.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEEAIAREPKGGRADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRKRSDELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLNQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1684..2601)
                     /locus_tag="WGR04_001618"
     CDS             complement(1684..2601)
                     /locus_tag="WGR04_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0795138.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFADGA
                     HQVNRLTDALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            2774..3574
                     /locus_tag="WGR04_001619"
     CDS             2774..3574
                     /locus_tag="WGR04_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0795139.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLHQDLLG"
     gene            3622..4398
                     /gene="mlaE"
                     /locus_tag="WGR04_001620"
     CDS             3622..4398
                     /gene="mlaE"
                     /locus_tag="WGR04_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ0795140.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            4449..4943
                     /gene="mlaD"
                     /locus_tag="WGR04_001621"
     CDS             4449..4943
                     /gene="mlaD"
                     /locus_tag="WGR04_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ0795141.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVGFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            4980..5570
                     /locus_tag="WGR04_001622"
     CDS             4980..5570
                     /locus_tag="WGR04_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ0795142.1"
                     /translation="MKKSSFISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALTKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            5627..5905
                     /locus_tag="WGR04_001623"
     CDS             5627..5905
                     /locus_tag="WGR04_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ0795143.1"
                     /translation="MHTELNNGTLHISGDVTVKTLTAAAFTRFRQQCRLKDTRAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            5902..6729
                     /locus_tag="WGR04_001624"
     CDS             5902..6729
                     /locus_tag="WGR04_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ0795144.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            6854..7216
                     /locus_tag="WGR04_001625"
     CDS             6854..7216
                     /locus_tag="WGR04_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ0795145.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7213..7599
                     /locus_tag="WGR04_001626"
     CDS             7213..7599
                     /locus_tag="WGR04_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0795146.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFLEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            7596..8084
                     /locus_tag="WGR04_001627"
     CDS             7596..8084
                     /locus_tag="WGR04_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0795147.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8166..8333)
                     /locus_tag="WGR04_001628"
     CDS             complement(8166..8333)
                     /locus_tag="WGR04_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ0795148.1"
                     /translation="MEDDVWPGRRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(8300..8674)
                     /locus_tag="WGR04_001629"
     CDS             complement(8300..8674)
                     /locus_tag="WGR04_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ0795149.1"
                     /translation="MGSKFFFLLLRFAGSVLPPSHMRGIVGRRVRGFLARRVSPHIGR
                     GVNIERGAYVFPDTVLGDGSGIGANCEICRGPVVGKNVMMEPECLFYSNNHKFDRSKN
                     ALRATRKSVRLRWRTMSGRGAG"
     gene            8684..8787
                     /locus_tag="WGR04_001630"
                     /pseudo
     CDS             8684..8787
                     /locus_tag="WGR04_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            8912..9127
                     /gene="rpmE"
                     /locus_tag="WGR04_001631"
     CDS             8912..9127
                     /gene="rpmE"
                     /locus_tag="WGR04_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEZ0795150.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT         2106 a         2637 c         2378 g         2084 t
ORIGIN      
        1 taaatttaat ccgctataca aagacaaaaa catcgacctc ggtcatgact gattgccgat
       61 gaagcaataa aaatgccgtc tgaacctgaa aacgggttca gacggcattt tctatcgggg
      121 ttttaaagcg gcattaaatg tcggtttcca aataaacgac ttgggtttgc agatattctt
      181 ccaaaccgtg tttgccgtcc gcgccgccga taccggattt tttccaaccg gcgtggaaac
      241 cctgcatggc ttcgaagttt tcgcggttga tgtaggtttc gccgaattgc aggcggcggg
      301 taacgtagaa ggcttcgttt aaattagtcg tataaacaga actggtcaga ccaaactcgc
      361 aatcgttagc cagtgcgatg acttggttga gcgtgtcgaa agcggaaacg ggcagcacgg
      421 ggccgaaggt ttcttctttc ataatgtcca tactgttgtc ggtgtcggtc agcagggtcg
      481 gctcgaagaa atagccgcgc ccttcggcgc gtttgccgcc gcagaccagt ttcgcacctt
      541 gtttgactgc ccgttccact ttttcggcaa cggctttgac ggcgcgctct tcaatcagcg
      601 gacccatttc cagcgcgcct gcttcggctt cggcagggtt gccgtagcgc acgcctttca
      661 tggcggcggt cattttttca atgaacgcgt ctttcaggct gctgtggaca tagacgcgct
      721 cggcgcagtt gcagatttga ccggtgttgc cgacgcgcga agccaagatg gatttcaccg
      781 ccaaatccaa atccgcgtct ttcaaaacga tggcgggggc tttgccgccg agttccagcg
      841 aaactttggt gatgttggcg gaagcggctt ccatcacttg gcggcctgct tcgacggagc
      901 cggtcaggct gaccatatcg acttgcggat gggcagacaa ggcattgccg atttccgcac
      961 ccggaccgtt caccacgttg aacacgcctg cgggcaatcc gaccgcatcg acgatttcgg
     1021 cgaagatgtg gcagttgatc ggggttacgc tgctgggttt gacaacgatg gtgttgcccg
     1081 ttactaaggc gggacccatt ttgcgggcaa tcaggaagaa agggaagttc cacggcaaaa
     1141 tgccggcaat tacgcccagc ggacgtttga acaataaaat attttcacgc gggcggtcgc
     1201 tttggatgat ttcgccttcg taacggcgcg cccattcggc ttggtaatcg agatagtcgg
     1261 cggtgaacat cacttccacg cgcgccaagt ctttggtttt gccgccttcg gcaacaatgg
     1321 tgtcggtcag ctcgtcggag cgtttgcgta tgccttgggc gattttgcgc aaatacgcgc
     1381 cgcgttcgac cgcaggcaga cgttcccaag ccggttgcgc cgcacgcgcc gccgctacgg
     1441 cgcggtcaac gtccgccctg ccgcctttgg gttcgcgggc gatggcctct tcggtggacg
     1501 ggttcaatac gtcgcgccat tcgccgttga aatcgttttc aaagcgtccg ttgatgtaca
     1561 ttgccaattg tttcatttcg ggttctccgg ttttgtagtc agatgtagtt ttagtttatt
     1621 cccaaataaa ttgccgtaca agtttcttta cacgcggatt tcgggtttga aatcaaatgc
     1681 ggcttaccgg ttttcttttt tggtttccgc aggcgtgctg ccgaaatatt gcctgaatgc
     1741 ctgagtaaag ctggaaacgt ggcggtagcc gcacaaatac gcggtttcgc ctatgctttt
     1801 gcccccgttt tgcaacaggt agagcgcgtg ctgcatttgt ttgtggtgca gccactcgct
     1861 tgccgtaatg ccgaaatggt cgcgcatacg gcgttgcagc gtcctttcac tgatgttcag
     1921 cgcgtctgtc agccggttga cttggtgtgc gccgtcggca aacgcggcat ttagggtgcg
     1981 gctgaagtct tcagacggca tagcgtctgc ttccgccgtt tgccccgccg ccggcccgat
     2041 gccgtctgaa accgtgtccc acaagtccga cagcagccgc aacacgtccg cctcgcggcg
     2101 caatgtttcg cccaaatgcc cctttgggac ggctttcagg caggatgccg ccaagccgcg
     2161 caggttcggg ggcaaatccc atatcctgac cggttcgcgg taaagcaggg gtgcgaaacg
     2221 cgcgtattcc ggacgcagca gccattgttc cataccttta attgtcattt tgaccgtttt
     2281 gccgcctcgg taaagatagc ggctgaacag gacttcttcc ccgacagcaa ttaggacaat
     2341 cttgccgccg tccgcatcga tttggaagcg gcagcggttg atgccgaagt ccaaactgcc
     2401 ttccagcaag agcacgaacg acacataagg ttcggcgagg cggctgctgc aaaaatcaca
     2461 ccgtgccgtt actgtgccgc cgtggatgga aatgccgttg gacaaagtat cgaaacggta
     2521 gctgccctcc acatagctgc cgccgccgct tttgactaga ttgacgaacc ggctgctgtt
     2581 caaatgttct gctttttcca tagttttctg ataaaggtta tcatttgaaa gataacattt
     2641 ttcgccacag caaacaatcc gccgccgaca acggcatttt cacaacattt accgtccgta
     2701 tgccgtctga aaattgcccg tcttcccgat aagccttaaa atacacgccg tcaaacctac
     2761 cgtccgtccg tacatgagcc catccccctt tatcgaaatg aaagacgtcg ccttcgcgta
     2821 tggcgaccgc ccgattctga agaacatcaa tttcagcatt ccgcaaggca attttgctgc
     2881 cgtaatgggc ggttcgggca gcggcaaaac cacgctgatg cgactgatta caggacagat
     2941 tcgtccgcag tccgggcagg ttttgattga aggacgggat ttggcgggct tttcggctga
     3001 cgagttgtat gaacaccgcc gccgtatggg cgtattgttc caacacggcg cgctgtttac
     3061 tgatttgtcg gtattcgaca atatcgcttt tccgatgcgc gaactgacgc gcctgccgga
     3121 agcggtgatt cgggatttgg ttttgttgaa attgaacgcg gtcggtctgc gcggtgtgga
     3181 aaacctgatg ccgtccgagt tgtccggcgg gatgtcgcgc cgcgtcgcgc ttgcccgcac
     3241 gattgcgctc gaccctgaaa ttatgttgta cgacgagccg tttaccggcc tcgatccgat
     3301 ttccttgggc gtgattgccc acttgatcag ccgcgtcaac aaggctttgc gttcgaccag
     3361 tattatggta acgcacgaca ttgaaaaatc tttggaaatc gtcgatcagg tgattttctt
     3421 ggcgcacggc gaaattatgt tctccggctc gccgcaggaa atgcgcgaac tggattcgcc
     3481 ttgggtgcgc cagtttgtcg gcgggctggc agacggtccc gtagcatacc gctatccggc
     3541 gcaaacgtcg ttgcatcagg atttgctcgg gtaaatgagc cggtgccgtc tgaaaccgtt
     3601 gaacttcaca tatagaaatt tatgaatttt atccgttccg tcggggcgaa aaccctcggc
     3661 cttattcaat ccctcggcag tattacgctg tttctgctga acattctggc gaagtccggt
     3721 acggctttcg tccgtccgcg cctgagcgtg cgccaagtgt atttcgccgg cgtgctgtcg
     3781 gtgctgattg ttgccgtttc ggggctgttc gtcggtatgg ttttgggttt gcagggctat
     3841 acgcagttgt cgaaattcaa atccgccgat attttgggct atatggttgc ggcttcgctg
     3901 ttgcgcgagc tgggtccggt attggcggct attctgtttg ccagcagcgc gggtggtgcg
     3961 atgaccagcg aaatcggtct gatgaaaacg accgaacagc tcgaagcgat gaacgtgatg
     4021 gcggtaaacc ctgtcgcccg cgtggttgcg ccgcgctttt gggcgggcgt gttttccatg
     4081 ccgcttttgg cttcgatttt caacgtggcg ggtattttcg gcgcgtattt ggtcggtgta
     4141 acctggctgg gcttggacag cggcattttc tggtcgcaaa tgcagaacaa catcacgata
     4201 cattacgatg taatcaacgg tctgatcaaa tccgccgcgt tcggcgtggc ggtaacgctg
     4261 attgccgtgc atcagggctt ccactgcgtc ccgacctcgg aaggcatttt gcgcgccagc
     4321 acgcgcacgg tggtttcgtc cgccctgacg attttggcgg tcgattttat attgaccgcg
     4381 tggatgttta cagattgaca gggcgtctga agacgaaacg cggaacatcg gatattcaag
     4441 gaactttaat gaaaaagaac atattggaat tttgggtcgg actgttcgtc ctgattggcg
     4501 cggcggcggt cggctttctc gctttccgcg tggccggcgg tgcggcgttc ggcggttcgg
     4561 acaaaactta cgccgtttat gccgatttcg gcgacatcgg cggtttgaag gtcaatgccc
     4621 ccgtcaaatc cgcaggcgta ttggtcgggc gcgtcggcgc tatcggactt gacccgaaat
     4681 cctatcaggc gagggtgcgc ctcgatttgg acggcaagta tcagttcagc agcgacgttt
     4741 ccgcgcaaat cctgacttcg ggacttttgg gcgagcagta catcgggctg cagcagggag
     4801 gcgacacgga aaaccttgct gccggcgaca ccatctccgt aaccagttct gcaatggttc
     4861 tggaaaacct tatcggcaaa ttcatgacga gttttgccga gaaaaatgcc gacggcggca
     4921 atgcggaaaa agccgccgaa taaacgtatg ccatcatatt caaactagga cacttaatca
     4981 tgaaaaaatc ctccttcatc agcgcattgg gcatcggtat tttgagcatc ggcatggcat
     5041 ttgccgcccc tgccgacgcg gtaaaccaaa tccgtcaaaa cgccactcaa gtattgagca
     5101 tcttaaaaag cggtgatgcc aacaccgccc gccaaaaagc cgaagcctat gcgattccct
     5161 atttcgattt ccaacgtatg accgcattgg cggtcggcaa cccttggcgc accgcgtccg
     5221 acgcgcaaaa acaagcgttg accaaagaat ttcaaaccct gctgatccgc acctattccg
     5281 gcacgatgct gaaattaaaa aacgccaacg tcaacgtcaa agacaatccc atcgtcaata
     5341 aaggcggcaa agaaatcatc gtccgcgccg aagtcggcgt acccgggcaa aaacccgtca
     5401 acatggactt caccacctac caaagcggtg gtaaataccg tacctacaac gtcgccatcg
     5461 aaggcgcgag cctggttacc gtgtaccgca accaattcgg cgaaatcatc aaagcgaaag
     5521 gagtggacgg actgattgcc gagttgaagg ctaaaaacgg cagcaagtaa tccataagcc
     5581 atgccgccag actgaagatg gcatggattt ttccctaccg aacactatgc atacagaact
     5641 caacaacgga acactgcaca tcagcggcga cgtaaccgtc aaaaccctga ccgccgccgc
     5701 ctttacccgc ttcagacagc aatgtcgtct gaaagacacc cgcgcggttg atttgagcgg
     5761 cgtcggacgc gccgattccg cctgcgtgtc gctgctgctc gaagtgctgc gcggttgcaa
     5821 aggcagcgtc aggctgaccg gcattcccga atccgtgcgc gcgctgtccg aactgtacga
     5881 aatcaaagac tggctgaaat catgaaaaaa accgcctatg ccttcctcct gctgatcggg
     5941 ttcgcttccg cccctgcatt tgcagaaacc cgccccgccg acccttatga aggctacaac
     6001 cgcgccgttt tcaaattcaa cgaccaagcc gaccgctaca ttttcgcccc tgccgcgcgc
     6061 ggctaccgca aagttgcgcc gaaacccgtc cgcgccggcg tgtccaattt ttttaacaac
     6121 ctgtgcgacg tggtcagctt cggcagcaat atcttgcgct tggacatcaa acgcgcaagc
     6181 gaagaccttg tccgcgtcgg catcaacacc actttcggtt tgggcgggct tatcgacatc
     6241 gccggcgcgg gcggcattcc cgacaataaa aacaccttgg gcgacacgtt tgcttcgtgg
     6301 ggatggaaaa acagcaatta tttcgtgttg cccgtcttag ggccgtccac cgtccgcgac
     6361 gcgctcggca cgggtattac ctccgtttat tcgcccaaga atatcgtctt ccgcacccct
     6421 gtcggacgct ggggcacgac tgccgtatcc gccgtcagta cgcgcgaagg tctgctcgat
     6481 ttgaccgaca gtctggacga agccgccatc gacaaataca gctacacgcg cgacctctat
     6541 atgaaagtcc gtgcgcggca gaccggtgca acacctgccg aaggtacgga agataacatc
     6601 gacatcgacg aattggtcga aagtgccgaa accggcgcgg cggaaactgc cgttcaagaa
     6661 gattccgtat ccgaaacaca ggcagaagca gcaggggaag ccgaaacgca acctggaaca
     6721 caaccttaaa gccatgccgt ctgaaaatcc gtgttcggac ggcattccct tctgtcggaa
     6781 cggacagtcc gaaaccacag attgggctat aatctctttt ttgcgcatac cgcgcgacag
     6841 caaaggaaaa accatgtacg aagtcaaccg cagcgtattc gtcctcatcc ctttagaacc
     6901 tttttggaac tggctgcaaa ccctgccggg caaccacctc gacggactga ctcttgaaga
     6961 catccaagcc gacgccaatt cctaccttgt ccgcccgtgc gaaaccgccg acgaagtatg
     7021 ggacgaaatc gaagcccgtt ttgaagacat tttcgccgcc gaacttgccg actggtgcga
     7081 agacgaacgc gagtggcctg cgctcgatgc cgacattttc aacgaatggt tcgacatcca
     7141 gctttctacc gtcatcaccg acctcgaaca cgaaccgctt gcccgcgaag ccttccaacc
     7201 catcaacctg aactgatgaa gctgaccgtc cgcaactacc atctcgacgg ctacggccat
     7261 gtcaacaatg cgcgctacct cgaatttctc gaagaagcgc gttgggcgtt ttttgaagaa
     7321 cgcggactgc tgcacgagtt ggcaggcctc atactgattg tcgcccgcat cgacatccga
     7381 tacagccgcc ccgccgtcga aggcgacgta ttgcagtttt catgccgcct gaaaaccccc
     7441 ggcacgcgcc gtattgtgct gacccagacg attacactgc caaacggcaa aaccgccgca
     7501 gaagccgaca tcaccctgat gcccgtccac gccgccacac aacgcaccgt cagcctgccc
     7561 gccacccttg cccgcgcatt ggaagccttg tccgaatgaa aaaaatactc accgccgccg
     7621 tcgtcgcact gatcggcatc ctccttgcca tcgtcctcat ccccgacagc aaaaccgcgc
     7681 ccgccttctc cctgcccgac ctgcacggaa aaaccgtttc caacgccgac ctgcaaggca
     7741 aagtaaccct gattaatttt tggtttccct cctgtccggg ttgtgtgagc gaaatgccca
     7801 aaatcattaa aacggcaaat gactataaaa acaaaaactt ccaagtcctt gccgtcgccc
     7861 agcccatcga tccgatagaa agcgtccgcc aatatgtcaa agactacggt ttgccgttta
     7921 ccgtcatgta tgatgcggac aaagctgtcg gacaggcgtt cggcacacag gtttatccga
     7981 cttccgtcct tatcggcaaa aaaggcgaaa tcctcaaaac ctacgtcggc gaacccgatt
     8041 tcggcaaact ctaccaagaa atcgataccg cgctggcaca atagcgatgc cgtctgaaac
     8101 gcaaaaaaac agaaacgatg ttcctgtttt ttcagatcat ccgccccgac acgttcagac
     8161 ggcattcaac cttccggcag attctttttc accactgccg gattgcctgc cgccaaagag
     8221 tagggcggaa tgtcttttgt aaccaccgcg cctgcgccca cgaccgaacc gcgtccgacg
     8281 gttacgcccg ccattacaat caccctgcgc cccggccaga catcgtcctc caacgtaatc
     8341 ggacggattt ccgtgtagcc ctcaaagcgt tttttgaacg gtcaaacttg tggttatttg
     8401 aataaaacag acattccggc tccatcatca catttttgcc gaccaccggc ccacggcaga
     8461 tttcacagtt tgccccgatg cccgagccgt cgcccaaaac cgtatccgga aacacatacg
     8521 ccccgcgttc gatattgacc ccgcgtccaa tatgcggaga aacccgccgc gccaaaaaac
     8581 cgcgtacccg tctgccgacg atgccgcgca tatgcgacgg cggcaacacc gaaccggcaa
     8641 aacgcagcag caggaaaaag aattttgatc ccatcaaacc tccctcaaaa gaacttttta
     8701 tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtaca gatagtacgg
     8761 caaggcgagg caacgctgta ctggtttttg ttaatccact atacatttta tcgaatatcc
     8821 tcttgacgaa acaacagaaa atagcgagaa tgccgagctt gtctgaagca taggtacatc
     8881 ccctatgccc gcttttttga aaggaaatca aatgaaacaa ggtattcacc cgaactacca
     8941 cgaagtcaac gttacctgct cttgcggcaa caaattcgca accaaatccg cgatggaaaa
     9001 agaaaacttc aatatcgagg tttgctccct gtgccacccg ttctataccg gcacccaaaa
     9061 aatcgtcgat accaccggcc gcgtggacaa attcaacaac aaattcggca acctgttcaa
     9121 acgctgattg ccgctttgca agaattaagc cctgccatca tttcgatggc agggcttttt
     9181 aatatttggc acactgactt cttat
//
LOCUS       DAWFJR010000078         8892 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWFJR010000078 DAWFJR010000000
VERSION     DAWFJR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8892
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.078"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(76..270)
                     /locus_tag="WGR04_001632"
     CDS             complement(76..270)
                     /locus_tag="WGR04_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ0795151.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(260..2341)
                     /locus_tag="WGR04_001633"
     CDS             complement(260..2341)
                     /locus_tag="WGR04_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ0795152.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEILAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            2817..3197
                     /locus_tag="WGR04_001634"
     CDS             2817..3197
                     /locus_tag="WGR04_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ0795153.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEVYDDGGRGHMSSVQNQARPADF
                     RAFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            3665..3829
                     /locus_tag="WGR04_001635"
     CDS             3665..3829
                     /locus_tag="WGR04_001635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795154.1"
                     /translation="MYKKVFFLNDRDCARAIFRHRPDVALPDRMLQTASEGFAVLEYE
                     DDNVREIDGI"
     gene            3862..5295
                     /locus_tag="WGR04_001636"
     CDS             3862..5295
                     /locus_tag="WGR04_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ0795155.1"
                     /translation="MNEYYQLIKHPDISLSPISDGIGVDNPATGEILAYVRKTDSDKL
                     KNLIQKAAAAQKLWAAKTALERADVLWRWYFQIKENKEALARLMTMEQGKSLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALAVLAYEAGVPQDLLPVVSGRASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDAFCRKLSEKAAALKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALSKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEVEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            5405..6193
                     /locus_tag="WGR04_001637"
     CDS             5405..6193
                     /locus_tag="WGR04_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ0795156.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVVEAFEKDRINVVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            6561..8117
                     /locus_tag="WGR04_001638"
     CDS             6561..8117
                     /locus_tag="WGR04_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0795157.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            8134..8880
                     /gene="surE"
                     /locus_tag="WGR04_001639"
     CDS             8134..8880
                     /gene="surE"
                     /locus_tag="WGR04_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ0795158.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEQALWTLLAHFFKNPPQSPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHTD"
BASE COUNT         2080 a         2243 c         2559 g         2010 t
ORIGIN      
        1 tgatttttgt taatccacta tattataaaa attccgtctg aagcggcgtt cagacggaat
       61 ttgtttcaag caggcttaac agcagcgtcc gccgtttgcg ccgcagcggc gtttgcggca
      121 atagtcttga aactgcagct tggtcatcac gggggcgttg ggattatgtt tgcgctgctg
      181 tgcaacgtag ttttcataat cgggcacgcc tgccatcaag tttgccgtca gcttgatggt
      241 tttccaccaa gacgcgagct tatgcttcac tttgtgcctc cggctgtttg ccgtcgcggt
      301 acaccgccgg gatttctttg gcggtcggcc agccgacttt gcgtgctttg agggcggtac
      361 gcaaaccgta cgcggcgaca atcacgacaa ccgacaagaa gaggatggtc agaccggcat
      421 taatcttgtc gttgaagatg atttgcgcca tttcgccgat gtctttggca ggcgcaagga
      481 tttcgttttt agccaatgcg tcgctgtatt tgccggcgtg ggcaaggaag ctgatgcgcg
      541 ggtcgctgtg gaacagtttt tgcaggccgg cgtagcaggt tacgaacagt acgccgacgg
      601 cgggaacgag taccacccag acataacggt cgcgtttcat cttaatcagc accacggcgc
      661 acataatcaa ggctacgcct gccagcattt ggttggcgat gccgaacaaa ggccagagcg
      721 agttgatgcc gcccaacggg tcggttacgc cggtgtagag aaagtagccc cacaatgcca
      781 cggcgaagaa ggtcgcaatc aggttggcgg ggatggagtc ggtgttgccg aaaggtttgt
      841 agaagatgct gcccaagtct tgaatcataa aacgtgcgac gcgcgtaccg gcatcgacgg
      901 cggtcaggat gaacaaggct tcaaacaaca gggcgaagtg ataccagaac gccatcatcg
      961 cctcgcccgg aatcaggcgg ctcataatgt gcgccatacc gactgcgagg gtgggcgcac
     1021 cgcctgcacg ggaaaggatg gtgttttcgc ccacttcctt agcggtgtgc aacagggttg
     1081 ccgcatcgac agggaattgc agcttggtgg taatcacttc ggcggcggta ttggcatccg
     1141 taccgatcag ggcggcaggg ctgttcatgg cgaagtacac gccgggatca agcgatgcgg
     1201 cggcggcaag cgccataatg gctacgaaac tttccatcaa cataccgccg taaccaatca
     1261 tacggacgtg ggtttcgttt tccagcattt tcggcgtggt gccggaggaa atcagcgcgt
     1321 ggaagcccga aaccgcaccg caggcgatgg tgatgaagag gaacgggaac aatgcgcctg
     1381 agaataccgg acccgaaccg tcgataaagt gggttacggc aggcatttgc aaagcgggat
     1441 tgacgatgac gatgcccaaa gccaaggccg caatcgtacc gattttcagg aaggtggaga
     1501 gatagtcgcg cggagtgagc agcaaccata cgggcaatac ggaggcgaca aagccgtaaa
     1561 tcataatcgc ccaagtgagc tggatgccgt caaggtcgaa ccaatgcccg atggaacttt
     1621 tcgccacatc ttcgccgtaa attaccgcca gcatcagcaa aataaagccg acgatggaaa
     1681 tctcgccgat tttgcccgga cggatatagc gcgtgtaaat acccataaac agggcaatcg
     1741 gcatcgttgc ggcaatggtg aacgtacccc aagggctgtg aaccaatgct tttacgacaa
     1801 tcaacgccaa caccgccata atgatgacca taatcatcaa aataccgatg gaggcaatca
     1861 cgccggggac agtgccgagt tcctgtttca caatatcgcc caaagactta ccgtcgcggc
     1921 gcatagagac gaacaagacc atcatatcct gtaccgcgcc ggcaaacacc acgccgaaga
     1981 taatccacaa agtaccgggc aggtaaccca tttgcgccgc caaaaccggg ccgaccaagg
     2041 ggcccgcgcc ggcaatcgcg gcaaagtggt gtccgaacaa tacgcccttg tgcgtcggaa
     2101 cgtagtccaa gccgtcgttg tggcgttctg ccggagtcag gcggttagga tcgagccgca
     2161 ttacgcggtt ggcgatgtag aggctgtaaa aacggtaggc gatgcagtaa acggatatgg
     2221 cggcggtaac catccatacc gcgctgacct gttcgcctcg gctgagagcc agagtggtaa
     2281 aggatgctaa gccgaccagt accactatgc cccaaatgag gaaggttttg agtgatttca
     2341 tcgaataaat ccttatctca cactgtcgga atatgcctga acggcgggaa acggatgacc
     2401 gcctgccggc acgggcacaa accggagtct gaatgattga cgaagtatga aacagtgcgc
     2461 ttgtcgggat ttgtgcctta tgcccgcatc aaacagtgca gggatgcggg cgcacaaaac
     2521 gttaaacgcc gaataggatt ttaacgcaaa ttagcacact gatagcggtt tttacttgga
     2581 atttggaaaa aatttacatt ccttcgggcg gacaggcggg tttggacggc atcgtcaggc
     2641 aaaaggcggc gccggaagac cggtaaagaa ggggcgcgca atccggatta ttgattcatc
     2701 gcgggtaaat tccggttatc gggcttgtgt gtttgcgcgt ccgtttatag tatggcgttg
     2761 ccgcagcttg gaatcagggc ggttgtttca tatcttattt ttattgggga gcttttatga
     2821 atatcaggtt tttcgcgctg accgtaccgg ttttgtcttt ggcggcctgt gccgtgccgg
     2881 aggtgtatga tgacggcgga cgagggcata tgtcgtccgt tcaaaaccaa gcccgcccgg
     2941 cagattttcg ggcgttttca tgcgagaacg gtttgtctgt gcgcgtccgc catttggaca
     3001 gcggcaaagt cgcgttgcgg ctggacggca ggcgtgccgt cctctcttcc gacgttgccg
     3061 catccggcga acgctatacc gccgaacacg gtttgttcgg aaacgcaacc gagtggcacc
     3121 agaaaggcgg cgaagccttt ttcggcttta ccgatgccta cggcaattcg gtcgaaactt
     3181 cctgccgcgc ccgttaaacg gggcagcctg tttgaaccgc cgtccggatt tttcgggcgg
     3241 ttttttgtgt cggatttgtt ttggctgtcg gctggtttcg gcggttttcg ggcgtggcgg
     3301 attatttttc gtttcgttcc gggtttatcc tatcggcagc ggctcaatgc caactttaaa
     3361 cctgctccga tttcttcagg gctgttatcc aatgataaaa tatagtggat taacaaaaat
     3421 caggacaagg cgacgaagcc gcagacagta cagatagtac ggaaccgatt cacttggtgc
     3481 ttcagcacct tagagaatcg ttctctttga gctaaggcga ggcaacgctg tactggtttt
     3541 tgttaatcca ctataatgtt tggatttttg ccatcctcta ccacacgttg caactgcaat
     3601 ctttgctcct tatatattgc accccatcaa agggctgcat tacttttctt tctaaagatt
     3661 accattgtat aaaaaagtat ttttcttaaa cgacagggat tgcgcccgcg ccatattcag
     3721 acaccgccca gatgttgcgc tgcccgatcg gatgcttcag acggcatcgg aagggtttgc
     3781 agttttggaa tatgaagatg ataatgtccg cgagattgac ggcatttgaa agcggttatt
     3841 ccgcataccg gaggacacga aatgaacgaa tattaccaat taatcaagca tcccgatatt
     3901 tccctttccc cgatttcaga cggcatcggg gtcgacaatc cggcaacggg cgaaattttg
     3961 gcgtatgtcc gcaagacgga ttcggacaag ctgaaaaacc tgattcaaaa agcagctgca
     4021 gcacaaaaat tatgggcggc aaaaactgcg ttggaacgcg ccgatgtgtt gtggcgttgg
     4081 tattttcaga ttaaagaaaa caaagaagca ttagcgcgcc tgatgacgat ggagcagggc
     4141 aaaagcctga ccgaggcgcg tggcgaaatc gattatgcgg cttcgtttgt gcgctggttt
     4201 gccgaagagg cgcggcggat tgacggcgat gtgttaacga gtgtgaaagc gtcgcaaaaa
     4261 ctggttgtgt tgaaacagcc tgtcggtgtt accgccgcga ttacgccgtg gaacttcccg
     4321 tctgcgatga ttgcgcgcaa ggctgcgcct gctttggcgg tgggttgcgc gatgatcgtc
     4381 aaacctgcat cgctcacgcc tttgagtgcg tatgcgttgg ccgtgctggc ttatgaagcg
     4441 ggcgtaccac aggatttgtt gcccgtcgtc agcggtcgcg catcggaaat cggccgtgaa
     4501 tttgccacga acccgattat ccgcaaaatc agcttcaccg gctctaccga agtcggtgcg
     4561 aaaatttttg ccgacagcgc ggcggacatt aaaaaactca gtttggaact gggcggcaac
     4621 gcgccgttta tcgtgtttga cgatgccgat ttggacaaag ccgtcgaagg tgcgctcgcc
     4681 agcaagttcc gcaacagcgg tcagacctgc gtctgcacca accgcgttta cgcccaatcg
     4741 ggcatttacg acgcattttg ccgcaaattg agtgaaaaag cagccgcgct caaattgggc
     4801 aacggcttgg aggatggcgt caaccaaggg ccgctgattg aggaaaaagc ggtagaaaaa
     4861 gtcgagcagc acatcgccga cgcgctctca aaaggcgcgg tctgtctgac cggcggcaaa
     4921 cgcagcgcat tgggcggaac gttttttgag ccgaccgtct taagcggcgt aacggcgcaa
     4981 atggcggtgg ctcgcgaaga aaccttcggg cctttgtgtc cggtattccg ttttgaaacc
     5041 gaagtcgagg tcatcgaggc tgcgaacaat acggaatacg gtttggcggc ctaccttttc
     5101 accgccgaca ccgcccgcca atggcgcgtc ggcgaagcct tggaatacgg tatggtcggc
     5161 atcaatacgg gcttaatcag caatgaagcg gcaccgttcg gcggcgtgaa acgcagcggt
     5221 ttgggacgtg aaggcagcaa atacggtgcg gacgaatatc tggaattgaa atatctgtgt
     5281 atggatgtcg ggtgacggat gccgtctgaa cggcagggtt tcagacggtg ccgcatttta
     5341 agcagtctct atctgttgta caatgcgccc tgtttttacg gttatttttg atgtgaatta
     5401 agatatgatg gaaaacggaa aaacagtccc gagatggcgt tttgccttga aaagtgcggg
     5461 ctggcacctc ttaatcagcc tgtcggttgc agggctggcg gcattgctgg tttttaaggt
     5521 ttggtatcct tatccttatg ccgagctgac gggagggctg tcgctttatc agctggtggt
     5581 ggctgtcgat attgtatgtg gtccgctgct gactttaatt ttggcaagcc cgaagaaaaa
     5641 gacaaaggca cgcatggtcg atttttccat ggtcggcatc atccagctgg cggctttggt
     5701 gtacggtctg cacagcgttt ctttggcgcg tcccgtggtg gaggcgtttg aaaaagacag
     5761 gattaatgtt gtgaccgccg ccgaagtggc ggttgaagac ctgcacaaag cgccggaagg
     5821 gctgcaaagc ctgtcgtggt tcggcatccg ccgcattgca ttgaaagaac ctgaggatgc
     5881 ggatgagaag aacaagacgc tggatttgtc cctgaaaggt atcgagccga gtatgcgtcc
     5941 cgaccggtgg ctgccgtatt ccgacaagga agcagaagaa atccgcaaac atctgaaacc
     6001 gctgaaagtc ttggcggatg cgagaaaaac gacggttgcg gacattctga aacaggcagg
     6061 tctcgccgaa ggggaggagc tgtattacct gccgtttacc agcagcaggc agaaagagtg
     6121 gatagtcatt accgataaag agggcaacac caaaggctac gcgccgatag acggcttcat
     6181 catcacccct taagcgttgg gactccgtcc gcactcgaac atccgttctt cgcggcggta
     6241 gaatcagact gtatttgaga ggggcgcaac ccctcaatca gggcaacaca taggggaagg
     6301 ctttatgtgc cgtccgtgtg ttgaaacatt tcaaaataag ctcatgccgt ccgaacatcc
     6361 tttcagacgg tatggcattt tgccattgcc ccgataagct gttaaactat ttaaattatt
     6421 tcgaccgaag gtacacgcac atgaaagacc ccgagcagag cagcaagccc gcccgccgtt
     6481 ttttgtgcgt accgtctgtt caggcagctt gagcgcttca ggctgccttt tcgcgtttga
     6541 acttgaggaa tacgaaaatc atggatttca gttggttggc agaaccgcat acctggatag
     6601 gttttgccac gcttttggtg ttggaagtcg tattggggat agacaacctt gtctttgtgg
     6661 cgattttggc aaacaaggtc cagcccgcac ggcgcgaccg cgcacggatt atcgggctgg
     6721 ggctggcagt cgtcatccgc atcattatgc ttgcttttat ggcgcacatc atcacgctga
     6781 ccgagccgct gttccaaatc ggcggcctcg ccgtttccgg caaggacatg attatgctcg
     6841 cgggcggtat tttcctgctt tacaaagcca ccaccgaact gcatgaacgc ctcgaagggc
     6901 acaaccgttt tactgttgcc gacaaccaaa aaaaacacgc gccgttttgg ggcgtggtcg
     6961 cgcaaatcct gatactggat gccgtgtttt ccatcgattc ggtcattact gcggtggcga
     7021 tggttgatca tatcgtcgtg gcgatgggtg cggtcgtcgt cgcaatggct gtaatgattt
     7081 ctgccagcaa actcttgacc gaatttgtcg acagacaccc taccgtcgtg atgctctgcc
     7141 ttggtttttt gttgatgatc ggtttcagcc tgattgccga agccttccat ttccacattc
     7201 ccaaaggcta cctctacgcc gccatcggct tctcgatttt aatcgaactg tttaaccaga
     7261 tttcccagcg caacagccgc aaaaacgact acatcggcag ctcgtggcgc aagcgcaccg
     7321 ccgaaaacgt tttgggtatg atgggcatac gcgaaagcgt gcttgccgat gcgggcggcg
     7381 aatccgggga cgacgcgcat tttgaagaaa acgaaaaatc gatgatacgc agcgtgctga
     7441 cgcttgccga acgcccgatt atgggggtga tgatcccacg ccgcgacatc gaacggctgg
     7501 acatttccca aagccgcgaa gaacagtgtg cccaactgca aaatacgcct tacagccgcc
     7561 tgctcgttgt cggtaaggcg ggcgtggacg aacctttggg ctacatcaac aaaaaagacc
     7621 tgctgtccca actgctggaa acaggcggtc tcgacattca gacggcattg cgccagccac
     7681 tcgtcctgcc cgacagcacc accgcgctgg gcgcaatcga actcttccgc caaagcagcg
     7741 cggattatgc tttggtggtg gacgagttcg gcgcggtatt gggcatggta accatgaaag
     7801 acctgctcga aaccatcgca ggcgagttcc ccgaagaatt tgagcgcgaa gaagaaccag
     7861 ccgttcaggg gaatcccgat gaaagcctga cggtggaagg cgcgttggaa tatgtggagc
     7921 tggcatccca actcaatctg ccgcagcagg aggaagatgc cgatttccat acggttgccg
     7981 ggctgattat ggaagaattg caaaccatcc ccgatgtcgg cgattttgcc gatttccacg
     8041 gctggcggtt tgaagtggtc gaaaaagaag ggcagcgcat cgagcgggtc aaaatcacca
     8101 aattgcccga agaataagca ttcaggatag aaaatgaacg ttttgatttc caacgacgac
     8161 ggctacctct ccgaaggtat tgccgttttg gcgcgcgtta cggcggaatt tgccaacgtc
     8221 agggtggttg cgcccgaacg cgacaggagc ggggtcagca attcgctgac gctggaacgc
     8281 cctttgcagt tgaaacaggc gcaaaacggg ttctactatg tcaacggcac gccgaccgac
     8341 tgtatccaca tcgggcagtc tgtattttcg gattttcagg ccgattttgt cttttcgggc
     8401 atcaaccggg gcgcgaatat gggggacgac acgctttatt cggggacggt tgcggcggca
     8461 accgaagcct accttatggg catacccgct gtggcgtttt ccttaaacga cgcttccgga
     8521 cgctattggg cgaccgcaga acaggcactg tggacattgt tggcgcattt tttcaaaaac
     8581 cccccgcagt cccctatttt gtggaacatc aatatccccg ccgttgcgcc ggaagatgtg
     8641 cggggcatta aaatcgcccg tttgggcagg aggcatcacg gtcagaacgt catccccgcg
     8701 cgcaatccgc gcggcgaaca gatttattgg atagggccgg tcggcgaagt ttccgatcgg
     8761 gaagagggaa cggatttcgg tgaatgcggc gcaggtttca ttaccgtaac gccgttgcaa
     8821 atcgacctga ccgcctatcc ggacatggcg gaaacggcgg cgttctggca tacggactga
     8881 ccgtttcatc aa
//
LOCUS       DAWFJR010000079         8718 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWFJR010000079 DAWFJR010000000
VERSION     DAWFJR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.079"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(77..595)
                     /locus_tag="WGR04_001640"
     CDS             complement(77..595)
                     /locus_tag="WGR04_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ0795159.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTKQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(615..2000)
                     /locus_tag="WGR04_001641"
     CDS             complement(615..2000)
                     /locus_tag="WGR04_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ0795160.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            2393..3934
                     /locus_tag="WGR04_001642"
     CDS             2393..3934
                     /locus_tag="WGR04_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ0795161.1"
                     /translation="MDGRRWVVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLAAFALGIFLLLDGGEGGK
                     QTETL"
     gene            3963..4562
                     /locus_tag="WGR04_001643"
     CDS             3963..4562
                     /locus_tag="WGR04_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ0795162.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            4559..5164
                     /gene="nadD"
                     /locus_tag="WGR04_001644"
     CDS             4559..5164
                     /gene="nadD"
                     /locus_tag="WGR04_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ0795163.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            5223..5609
                     /gene="rsfS"
                     /locus_tag="WGR04_001645"
     CDS             5223..5609
                     /gene="rsfS"
                     /locus_tag="WGR04_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ0795164.1"
                     /translation="MNEQELQDLQKMVGVAVNALGDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            5663..6133
                     /gene="rlmH"
                     /locus_tag="WGR04_001646"
     CDS             5663..6133
                     /gene="rlmH"
                     /locus_tag="WGR04_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ0795165.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWQQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE"
     gene            6208..6474
                     /locus_tag="WGR04_001647"
     CDS             6208..6474
                     /locus_tag="WGR04_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ0795166.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            6631..7314
                     /locus_tag="WGR04_001648"
     CDS             6631..7314
                     /locus_tag="WGR04_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ0795167.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            7438..7950
                     /gene="coaD"
                     /locus_tag="WGR04_001649"
     CDS             7438..7950
                     /gene="coaD"
                     /locus_tag="WGR04_001649"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ0795168.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            7982..8635
                     /locus_tag="WGR04_001650"
     CDS             7982..8635
                     /locus_tag="WGR04_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ0795169.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNRESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
BASE COUNT         1896 a         2308 c         2540 g         1974 t
ORIGIN      
        1 aatgtaatcc attctgttcc cgatgtttat gccgtctgaa acccatcctg tgtcgggctt
       61 cagacggcat attgcttcaa agcaggtttt ccgaggcaac ccagttcaga atatcggctt
      121 cgaccgcttc gggcgtgccg gggttggcga ttttgactcc gtattcgcct tcgcccagtt
      181 cctcgaggat ttccagttgc gaatcgagca tccctgcttt catgtaatgt cctttgcgcg
      241 acatcatgcg ctcgaggttg atgtcttgcg gcggactgag gtggatgaag gcagctttgc
      301 cttcggctcc gcgcagaatg tcgcggtagc cgcgtttgag ggcggaacag gttacgatgg
      361 tgtggttcgc accgttttgc gcctgtttcg tcatccagtc gcgcagattg cccaaccacg
      421 gatagcggtc ttcatcggtc agcggaatac ccgcgcccat cttgtcgcgg ttggcttggg
      481 tgtggaactc gtcgccttcg gcatagggac attgaccgag gtgtttctgc agggacagcg
      541 cggcggtggt cttgccgcag ccgcatacgc ccataacgac aaaatgcgta gtcatttact
      601 atcctttccg tctgtcagac gatggcgaac agcagtgcgg acaaggcaaa gccgatgagt
      661 gcgatgaggg tttggttgac cgtccaggtt ttcagcgtgg tcggtacgtc catgtccaag
      721 agacggccga ccagccagaa gccggagtcg ttgaagtggc tgcaaccgac cgaacctgcc
      781 gccgttgcca atacgataca ggcgagctgc cagtcggtaa agccggcggc ggcaacggca
      841 ggagccatca gcgcggcggc ggtggtcagg gcgacggttg ccgaaccttg cgcgatacgc
      901 agtgccaagg cgacaaggaa acagcccaaa aggacgggaa tgcccaaatc cgccatgctg
      961 tcggcgagtg ccttgccgat gccggaagcg cgcaaaacgc cgccgaacat accgcccgcg
     1021 ccggtaatca gaatcacgga acagacgggg gcgagtgcgc cgtccacggt tttttccaac
     1081 gcgctgccgc tttcgccgcg tttgcgtccc aagacaaaca gtgcgaccaa tacggaaatc
     1141 agaagggcga tcggtgtcga accgattatt tttgccgtct gaacccaggt ttcgtccgca
     1201 cttacgagtt tttcgctgat gagggccgat acgccggtat tcaggaaaat cagcagcatg
     1261 ggaatcagca tgatggcgac gaccgttcct gctttggcag gttctttcgg caggtcgttg
     1321 tcttgcgtgc cgccgctgag cagttcggga acgggaacat ggatggtgcg ccccaacact
     1381 ttgccgagca tatagccgct gaaataccat gtgatgaagg cggtcggcag acccaaaatc
     1441 aaaacttggc cgatgttcgc gccgtaaaat tcggaagcgg caatcgggcc cggatggggc
     1501 ggcaggaaga cgtgcatgac ggaaaatgcg ccgatggagg caagcgcgaa gggcagtacg
     1561 tcctgtttca tgcgccgtgc ggtggcgaac acgatgggca gcatgacgat tagtccggca
     1621 tcgaagaaaa tcgggaagcc gaaaatcagc gaggcaacgc ccagcgcgaa cggtgcgcgt
     1681 ttttcgccga acatccggat cagcgcgtcc gccagcgact gtgcgccgcc ggatgtttcg
     1741 accagacgtc cgagcatcgc gcccaggccg accagaagcg ccacgccgcc gagcgtgccg
     1801 ccgaagtttt tgaccagtac gtcgttgaca atgctgcctg tgggcaaacc ggttgccaaa
     1861 gccgtcagca ggctgacgat gaccagtgtc agcagcgcgt ggatgcggaa tttgacgatt
     1921 aaaatcagaa tgaggatgat tgccgccgcc gaaatgccca acagggtttg cgcggacagc
     1981 gtctgtgtcc agccgtccat ttgtaaagcc tttctctgta ataaaagtac aaaattgtca
     2041 ggttttttta agttgtgcga aaacgatagc acaaatcggg cggtaagttg tttgtctgaa
     2101 gttatattcc tgtttatttg aacgatttat agtggattaa caaaaaccag tacggcgttg
     2161 cctcgcctta gctcaaagag aacgattctc taaggtgctg aagcaccgag tgaatcggtt
     2221 ccgtactatt tgtactgtct gcagcttcgt cgccttgtcc tgatttttgt taatccacta
     2281 tatttcagac ggcgggagga aggcggatac ggtatgtagt cggattgaaa tatacggtat
     2341 gtgggggctt ttgggtacaa tcgcaacatc agtgtttcaa gtggggaagg cgatggatgg
     2401 acggcgttgg gtggtatggg gtgcttttgc cctgctgcct tcggcttttt tggcggtaat
     2461 ggtcgttgcg cctttgtggg cggtggcggc gtatgacggt ttggcgtggc gcgcggtgct
     2521 gtcggatgcc tatatgctca aacgtttggc gtggacggta tttcaggcag cggcaacctg
     2581 tgtgctggtg ctgcctttgg gcgtgcctgt cgcgtgggtg ctggcgcggc tggcgtttcc
     2641 ggggcgggct ttggtgctgc gcctgctgat gctgcctttt gtgatgccca cgttggtggc
     2701 gggcgtgggc gtgctggccc tgttcggggc ggacgggctg ttgtggcgcg gcaggcagga
     2761 tacgccgtat ctgttgttgt acggcaatgt gtttttcaac cttcctgtgt tggtcagggc
     2821 ggcgtatcag gggtttgtgc aagtgcctgc ggcacggctt cagacggcac ggacgttggg
     2881 cgcgggggcg tggcggcggt tttgggacat tgaaatgccc gttttgcgcc cgtggcttgc
     2941 cggcggcgtg tgccttgtct ttctgtattg tttttccggg ttcgggctgg cgctgctgct
     3001 gggcggcagc cgttatgcca cggtcgaagt ggaaatttac cagttggtca tgttcgaact
     3061 cgatatggcg gttgcttcgg tgctggtgtg gctggtgttg ggggtaacgg cggcggcagg
     3121 gttgctgtat gcgtggttcg gcaggcgcgc ggtttcggat aaggcggttt cccctgtgat
     3181 gccgtcgccg ccgcagtcgg tcggggaata tgtgctgctg gcgtttgcgg cggcggtgtt
     3241 gtctgtgtgc tgcctgtttc ctttgttggc aattgttgtg aaagcgtggt cggccggcga
     3301 atcgtggcgt gtgttaatgg aaagtgaaac gtggcaggcg gtgtggaata ctttgcgctt
     3361 ctcggcggcg gcggtgtttg cggcggcggt tttgggtgtg gtgtatgcgg cggcggcgcg
     3421 gcggtcggcg tggatgcgcg ggctgatgtt tttgccgttt atggtgtcgc cggtttgtgt
     3481 ttcggcgggc gtgctgctgc tttatccgca gtggacggct tcgttgccgt tgctgctggc
     3541 gatgtatgcg ctgctggcgt atccgtttgt ggcaaaagat gttttatcag cctgggatgc
     3601 actgccgccg gattacggca gggcggcggc gggtttgggt gcaaacggct ttcagacggc
     3661 atgccgcatc acgttccccc tcttgaaacc ggcgttgcgg cgcggtctga ctttggcggc
     3721 ggcaacctgc gtgggcgaat ttgcggcgac attgtttctg tcgcgtccgg aatggcagac
     3781 gctgacgact ttgatttatg cctatttggg acgcgcgggt gaggataatt acgcgcgggc
     3841 gatggtgctg acattgctgt tggcggcgtt cgcgctgggt attttcctgc tgttggacgg
     3901 cggcgaaggc ggaaaacaga cggaaacgtt ataatgtgaa ccctttttca gaggacggca
     3961 aaatgagcga gttggataac atccttgccc ataaccggca gtttgtcgag tcgggcgaat
     4021 atgaaaaata ctttaccgac aaataccccg aacgcgggct ggcagttttg tcctgtatgg
     4081 atgcgcggat tatcgggctg ctgcccgacg cgttgggttt gaaaaacggc gatgccaagc
     4141 tgattaaaaa tgccggcgcg ctggttacgc acccgtgggg ttcggtgatg cggagccttt
     4201 tggttgccgt gtttgaactg aaggtcagag agattatggt catcgcccat cacgattgcg
     4261 gtatgcaggg gctgaatgcc gaagaattcc tcgggcgcgt ccgggaaagc cggattcccg
     4321 aagaccgtat cgaaaccctg cgttatgccg gtatcgacct cgacggctgg ctgaccggtt
     4381 tcgacaacgt cgaagacagc gtgcgccaca cggtggacct tatccgtaac catccgctga
     4441 tgccgcgcca tatcgccgtt cacggactgg tcatccatcc cgttaccggc aaactgacgc
     4501 tggttgtgga cggcagtgtt tcagacggca tggacttatc ggaaggaatg gaaacatcat
     4561 gaagaaaatc ggattgttcg gcggtacttt cgacccgata cacaacggac atcttcatat
     4621 cgcccgtgcc tttgccgacg aaatcggctt ggacgcggtt gttttcctgc cgacaggcgg
     4681 cccgtatcac aaagacgcag cctccgcttc cgccgccgac cgccttgcca tggtcgaatt
     4741 ggcgacggca gaagacgcgc gttttgccgt cagcgattgc gacatcgtcc gagaaggtgc
     4801 aacctatact tttgataccg tccaaatctt ccgccagcag ttcccatccg cgcaactctg
     4861 gtggctgatg ggcagcgaca gcctgatgaa gctgcacaca tggaaaaaat ggcagatgct
     4921 cgtgcgcgaa accaatatcg ccgtcgccat gaggcagggc gacagcctgc accaaacccc
     4981 gcgcgaactg cacgcgtggc tgggcaagtc ccttcaggac ggcagcgtcc gcatcttgtc
     5041 cgccccgatg cataatgtgt cgtcaacgga aatccgccgc aaccttgccg gccaaggcgt
     5101 ttcagacggc atcccgcctg ccgccgcacg ctacatccgc gaacacggtt tgtatgaaaa
     5161 ataaagtcaa atcagtaaga aacggctata atgccgtctg aaaacatccc gttaggaaaa
     5221 taatgaacga acaagaactg caagacctgc aaaaaatggt cggggtcgcc gtcaacgccc
     5281 tcggagacat caaagccaaa gacatttccg ttctcgaaac ccaagacaaa acttcgctgt
     5341 ttgccagaat gattatcgcc agcggcgaca gtacgcgcca agtcaaagca ctggccaaca
     5401 acgttgccgt cgatttgaaa gaagccggtt ttgaaatcct cagcaccgaa ggcgacagcg
     5461 gcgaatggac gctcgttgat gcaggagacc tcgtcgtcca cgttatgctc cccgccgtgc
     5521 gcgacttcta tgatatcgac accctttggg gcggcgagaa accgagtttt cacgccggaa
     5581 tgcagaagcc gtggcacgcg gcagactgat tcccgatgcc gtctgaatgt tcagacggca
     5641 ttttcctgta agggagaaag cattgaacat caccgttttg gcagtcggca ccaaaatgcc
     5701 gcgctgggtt gatgaggccg tcgccgaata cgccaaacgc ttcggacgcg acgtcgccta
     5761 cgcactcaaa gaaatcaaac ccgaaaaacg cggcgcgggc gtgaatgccg cccaaggtat
     5821 ggcggcggaa gaaaaacgca tccttgaagc cattccgcaa ggcgcgttcc tcgtcgttct
     5881 tgacgaacgc ggcaaagcac cgacctccgt cgagctggcg gaacacctca aaagctggca
     5941 gcaaaacggc gaacacgtct gcttcgtcat cggcggcgcg gacggtatga ccgaccgcct
     6001 caaacagcag gcccgcatga tgatgcgcct gtccagcctg accctgccgc acgggatggt
     6061 gcgcgtcctt ctgaccgaac aactctaccg cgccgcctcc atcctgcaca accaccccta
     6121 ccatcgggaa taagaaggct tttgtccgca ctcccaatcg gttaaaatcc ccccgttatt
     6181 tctgaacaca cagcaaagga atccgctatg gcacgcatgg tattctgcgt caagctcaac
     6241 aaagaagccg aaggcatgaa atttccgccg cttcccaacg aattgggcaa gcgcattttt
     6301 gaaaacgtat cgcaagaagc gtgggcggcg tggacgcgcc accaaacgat gctgattaac
     6361 gaaaaccgtt taagcctcgc cgatccgcgc gcgcgcgaat acctggctca gcagatggag
     6421 cagtatttct tcggcgacgg cgcggatgcc gttcagggat acgttccgca ataacggttt
     6481 tccgttttga acacaggctg tccgaaactg cttcagacgg cctttaaaat acgccggaca
     6541 actttatctt cacgcaaccg gacaaatttt gacattgggc aatgttaaaa tcccccatta
     6601 ttcccaaccg cgctttcagg agcagatgat atgcaacacg acgtttacga ctacaccgcg
     6661 catacggttt ctaaaaacac cgtcctgcag aaaacctacc gcctgctcgg attttcattc
     6721 attccggctg ccgcaggcgc ggcacttgcc gccaatgccg gtttcaattt ttacgccgcc
     6781 ttcggttcgc gctggatagg gtttgccgtc gtgttggcgt ttttctacgg tatgatccac
     6841 ttcatcgaga aaaaccgtta cagcaatacc ggcgttaccc tgctgatggt attcacattc
     6901 ggtatgggcg tattgatcgg ccccgtgctg caatacgcgc tccatattgc cgacggtgcg
     6961 aaaatcgtcg gcattgccgc cgcgatgacc gccgccgtct ttttaacgat gtccgccttg
     7021 gcgcgccgaa cccggctcga tatgaacgcg ctcggacgct tcctgaccgt aggtgcggtc
     7081 attctgatgg tcgccgtggt tgccaatctg tttttgggta ttcccgcact cgccctgacc
     7141 atttccgccg gttttgtctt gttcagttcc ttaatgatta tgtggcaggt acgcaccgtc
     7201 atcgacggcg gcgaagacag ccatatcagc gcggcactga cactgtttat ctcgctttac
     7261 aacatcttca gcagcctgct gaacatcctg ttgtctttaa acggcgagga ttgatgtcgg
     7321 taaaacgccg tccgactatg ccgtctgaaa atgcttcaga cggcatttta ctttgggcat
     7381 acaatacgga tgcacacgca ccaacacaca gccggctatg aagccgggag gaatcaaatg
     7441 ttaccgaata caccgcgccg cgccgtttat gccggcagtt tcgatccgcc cacattgggg
     7501 catctgtgga tgatacggca ggcgcaatct atgtttgacg aactcatcgt cgccatcggc
     7561 attaaccccg acaaacgcag cacctatacc gtcgctgaaa ggcaggatat gttgtgcgat
     7621 attactaaaa tgtttcccaa cgtcagaacc gatgtatttg aaaaccgatt tctggtgcat
     7681 tatgcccgtg aggtagatgc aggattcatc gtgcgcggca tccgttctgc ttcggattac
     7741 gaatacgaac gttccatgcg ccacatcaac agcgacctcg cccccgaaat atccaccgta
     7801 ttcctcatgc cgccgcgcga aatcgccgaa gtgtcgtcca ctatggtcaa aggactggtc
     7861 gggccggaag gctggacgga aacgatccac cgctacgtgc cgcaagctgt gtacgaaaaa
     7921 atccttgccg aacatcaaca cgaaaattga acccttctat ttttgcaagg catatatgga
     7981 tatgttggaa attttgttcc gccatcaaga cttcgtcgcc atcaacaaac cgggcggcat
     8041 atccgtccac caagacagcg gcgagaccgg actggcccga acactcgcca tacagttggg
     8101 cgtagagcgt gtgtggctgc tgcaccgatt ggacaaacag accagcggca ttttactgtt
     8161 tgccctcaac agagaaagcg cgtccgccct tagcggacag tttgccggca aaagcatcaa
     8221 aaaaacctat ttggcactat ccgaccgcaa accgtccaaa aagcagggtt ggattaaagg
     8281 cggaatggaa aaatccagac gcggaatgtg gaagttgacg cgcaatacag aaaatatcgc
     8341 cgttacccga ttccacagca tcagtatcgc cgaaaaactg cggctgttca tcctggaacc
     8401 gcatacgggc aaaacgcacc aattgagagt ggcgatgaaa agtttgggca gtccgattct
     8461 gggcgacagc ttatacggcg gaacagaatc cgaaaccatg tttctgtatg catggaaaat
     8521 acaatttgcc taccaaaacc gacaaatcga aattgttgca cccttaaaaa acgaatggca
     8581 gactgaaaat atctctcatg cgctggaaga attttatatg gcaaaagcag gttaataaat
     8641 ataaattaat ttataaacaa tatgataaat atttttataa aaatttttca agaaaacggt
     8701 tgactgcaaa tctcttca
//
LOCUS       DAWFJR010000080         8546 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWFJR010000080 DAWFJR010000000
VERSION     DAWFJR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.080"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            120..1289
                     /gene="prpF"
                     /locus_tag="WGR04_001651"
     CDS             120..1289
                     /gene="prpF"
                     /locus_tag="WGR04_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ0795170.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAMILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKSKIPSDGICTVKIWQKNIGKTIIAHVPMQHGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLVNAGIPTIFLN
                     AVDLGYTGKELQDDINNDAAALEKFETIRAYGALKMGLISDVSEAATRAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            1704..2903
                     /locus_tag="WGR04_001652"
     CDS             1704..2903
                     /locus_tag="WGR04_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0795171.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSIVLSCLGERLTTPEAVI
                     TFSKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMDKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            3010..3630
                     /locus_tag="WGR04_001653"
     CDS             3010..3630
                     /locus_tag="WGR04_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ0795172.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKEVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRRGCGDTAVFGL"
     gene            complement(3757..4200)
                     /locus_tag="WGR04_001654"
     CDS             complement(3757..4200)
                     /locus_tag="WGR04_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ0795173.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            4374..4742
                     /locus_tag="WGR04_001655"
     CDS             4374..4742
                     /locus_tag="WGR04_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0795174.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEETKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            4787..5941
                     /locus_tag="WGR04_001656"
     CDS             4787..5941
                     /locus_tag="WGR04_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0795175.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(5998..6870)
                     /locus_tag="WGR04_001657"
     CDS             complement(5998..6870)
                     /locus_tag="WGR04_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ0795176.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHHCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVEELWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            6955..7767
                     /locus_tag="WGR04_001658"
     CDS             6955..7767
                     /locus_tag="WGR04_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ0795177.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGVDWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGDVPHLSAEGVSVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKYRVLDAV"
     gene            complement(7796..>8546)
                     /locus_tag="WGR04_001659"
     CDS             complement(7796..>8546)
                     /locus_tag="WGR04_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0795178.1"
                     /translation="LLFSSAAQAASEDGSRSPYYVQADLAYAAERITHNYPEPTGADK
                     DKISTVSDYFRNIRTHSIHPRVSVGYDFGGWRIAADYASYRKWKESNSSTKKVTEEIN
                     NNYKETQTKHQGNGSFHAASSLGLSAIYDFKLNDKFDKFKPYIGARVAYGHVKHQVHS
                     VESKNTIVTSKPTQGAAQGAPVPTGFVPKSAYHESHSISSLGLGVIAGVGFDITPKLT
                     LDTGYRYHNWGRLENTRFKTHEVSLGMRYHF"
BASE COUNT         1829 a         2317 c         2484 g         1916 t
ORIGIN      
        1 gccgtagatt gtggcgaacc ccgacagttt gcggaatcaa acggctttgg tcggagtggc
       61 agcctaatcc actataaaaa tcgtgggcag agcccacgct acataaggag aatctagaaa
      121 tgccgcaaat taaaattccc gccgtttact accgtggcgg tacatcaaaa ggcgtgtttt
      181 tcaaacgttc cgacctgccc gaggcggcgc gggaagcggg cagcgcacgc gacaaaatcc
      241 tcttgcgcgt actcggcagc ccggacccct acggcaagca gatagacggt ttgggcaacg
      301 ccagttcgtc caccagcaaa gccatgattt tggacaagtc cgaacgcgcc gatcacgatg
      361 tcgattacct tttcgggcaa gtttccatcg acaaaccttt tgtcgattgg agcggcaact
      421 gcggcaacct caccgccgcc gtaggcgcat ttgccatcga acaaggtttg gtcgataaat
      481 ccaaaatccc ttcagacggt atctgcacgg tgaaaatctg gcagaaaaac attggcaaaa
      541 ccattattgc ccatgttccg atgcaacacg gcgcagtttt ggaaacgggc gattttgagc
      601 tcgacggcgt aacgttcccg gcagccgaag tacaaatcga atttttagat ccggccgacg
      661 gcgaaggcag tatgttccca accggcaatt tggtcgatga aattgatgtg ccgaatatag
      721 gccgtctgaa agccacgctt gtcaatgccg gtattccaac cattttcctg aatgccgtag
      781 atttgggcta cacaggcaaa gagttgcaag acgacatcaa caacgatgcc gcggctctgg
      841 aaaaatttga aaccatccgc gcgtatggcg cgctgaaaat gggtttgatc agcgacgttt
      901 ccgaagccgc cacccgcgcc catactccga aagtcgcttt tgtcgcgccc gccgccgatt
      961 acaccgcctc cagtggcaaa accgtgaacg ccgccgacat cgatttgctg gtacgagccc
     1021 tgagcatggg caaactgcac cacgcgatga tgggtaccgc ctctgttgcc attgcgaccg
     1081 ccgccgccgt gcccggtacg ctggtcaacc ttgccgcagg cggcggaacg cgtaaagaag
     1141 tgcgcttcgg gcatccttcc ggcacattgc gcgtcggtgc agccgccgaa tgtcaggacg
     1201 gacaatggac ggccaccaaa gcggttatga gccgcagcgc acgcgtgatg atggaaggtt
     1261 gggtcagggt gccggaagat tgtttttaaa ttgacgtagc atgggtttgc ccgcgagcca
     1321 taaacaggtc gtctgaaaaa caagtaaaca tcaaatcact gaccattcct ttcccttgcc
     1381 ctgtggcgga aggcggcaaa tcacaaggaa gaacacggaa accccgataa aagacagctt
     1441 cccgtattac cgtcattccc gcgcaggcgg gaatccagac ctgtcaatat ggaggattgg
     1501 caggggaaaa caggtttcgt gagttctaca ttctggattc ccgccacagc ctgtcctcgc
     1561 gtaggcgggg acggaataac gatagaaaat gcggcatatg ctttgcccaa agaggccgtc
     1621 tgaaacacac tccgcccaac gcccatcctt tttcagacga ccccacacca aaaaaacaac
     1681 cacaaactac aaggagaaac atcatgtccg accaactcat tcttgttctg aactgcggca
     1741 gttcatcgct caaaggcgcc gttatcgacc gcaaaagcgg cagcatcgtc ctaagctgcc
     1801 tcggcgaacg cctgaccacg cccgaagccg tcattacgtt cagcaaagac ggcaacaaac
     1861 gccaagttcc cctgagcggc cgaaattgcc acgccggcgc ggtgggtatg cttttgaacg
     1921 aactggaaaa acacggtctg cacgaccgca tcaaagccat cggccaccgc atcgcccacg
     1981 gcggcgaaaa atacagcgag tctgttttga tcgaccaggc cgtaatggac gaactcaatg
     2041 cctgcattcc gcttgcgccg ctgcacaacc ccgccaacat cagcggcatc ctcgccgcgc
     2101 aggaacattt ccccggtctg cccaatgtcg gcgtgatgga tacttcgttc caccaaacca
     2161 tgccggagcg tgcctacact tatgccgtgc cgcgcgagtt gcgtaaaaaa tacgctttcc
     2221 gccgctacgg tttccacggc accagtatgc gttacgttgc ccctgaagcc gcacgcatct
     2281 tgggcaaacc tctggaagac atccgcatga ttattgccca cttaggcaac ggcgcatcca
     2341 ttaccgccat caaaaacggc aaatccgtcg ataccagtat gggtttcacg ccgatcgaag
     2401 gtttggtaat gggtacacgt tgcggcgaca tcgatccggg cgtatacagc tatctgactt
     2461 cccacgccgg gatggatgtt gcccaagtgg atgaaatgct gaacaaaaaa tcaggtttgc
     2521 tcggtatttc cgaactttcc aacgactgcc gcaccctcga aatcgccgcc gacgaaggcc
     2581 acgaaggcgc gcgcctcgcc ctcgaagtca tgacctaccg cctcgccaaa tacatcgctt
     2641 cgatggctgt ggcctgcggc ggcgttgacg cactcgtgtt caccggcggt attggcgaaa
     2701 actcacgtaa tatccgagcc aaaaccgttt cctatcttga tttcttgggt ctgcacatcg
     2761 acactaaagc caatatggac aaacgctacg gcaattcggg tattatcagc ccgaccgatt
     2821 cttctccggc tgttttggtt gtcccgacca atgaagaact gatgattgcc tgcgacactg
     2881 ccgaacttgc cggcatcttg tagccaaaaa agggacgagt ccgcacaatg ccgtctgaaa
     2941 ttttcagacg gcattttgtt tggattttgg gcgtacaata cggaaaacat gacgataagg
     3001 acacaaacca tggcacagtt tttcgctatt catcccgaca atccccaaga acgcctcatc
     3061 aagcaggcgg ttgaaatcgt caatcaaggc ggcgtggtgg tttatccgac cgattcgtgt
     3121 tacgccttgg gttgcaaact cggcgataag gcggcgatgg aacgcatact ctccatccgc
     3181 aaaatcgatt tgaaacacca cctgaccctg atgtgcgccg atttgagcga gttgggcacg
     3241 tacgccaaaa ttgacaacgt acagtttcgt cagcttaaag ccgccacgcc cgggccttat
     3301 acctttatct tgcaggcgac gaaggaagtg ccggcgcgca cgctgcaccc gaaacgcaaa
     3361 accatcgggc tgcgtattcc cgataatgcc attgcacaag ccctgctggg ggaattgggc
     3421 gagccgcttt taagctgcac cctgatgctg cccgaagacg gcgaaccatt gaccgatcct
     3481 tatgaaatcc gcgagcgttt ggaacacgcc gtcgatttgg tgattgacgg cggctggtgc
     3541 ggaaccgagc cgaccaccgt cgtcgatatg accgacggca cggaactggt gcgccggggt
     3601 tgtggcgata cggcggtgtt cggtttgtag ggaaaccgat gccgtctgaa gcatcggctg
     3661 ttcagacggc attgcgcgcc ttgccggcgg cagtccgaaa tgccggcgcg tatcgcgctc
     3721 ggtcggaata tccgtttgaa acggcatttt gatgcattac tgcaccgcaa tcggaattct
     3781 cggttcgtag agcaggtcgt aggtcggctt gttgagcagg tcttggagcg tgaaaccgtc
     3841 cagatacgtg aaaaacgact tcatcgcgcc gccgagtatg cccgtcagcc ggcaggacgg
     3901 tgtaatcagg cattcgttgt tctcgcccat gcactcgacc agctgcatcg gttcgaggtg
     3961 gcggacaacc gagccgatgt tgatgcggtc gggcggtgcg gcaagccgca gaccgccgcc
     4021 ttttccgcgc acactgtgga ggaagccgcc tttgaccagc gcggtaacga ccttcatcag
     4081 atggcttttg gaaatgccgt aggttacggc gatggtactg atgttgacca gcgcatcgtc
     4141 gttgatggca gtgtagataa ggacgcgcag cccgtagtcc gtatgttgtg tcaaatacat
     4201 gattttctcg gtatggattg ttattcttat cggtacggtt taaggttcac ggacaatacc
     4261 ttaatggttg aaaccctgtc cgtcggggcg gtagaatgca gcctgtctgc ggcggtatgc
     4321 cgtctgaaac atccgcgcta ccgtttgaga atttgttatt gtaactcaaa atcatgaaac
     4381 cgttgaaacg acatcccgcc cttatcgggc tttcgcgtga ccaccaccat tcgctttccc
     4441 tgtgcgtgcg tctgttgcgg acgccggaag aaaggcatcg ggacgaactc gaaccgcatt
     4501 tttccgaatt ggaaacccat tttcgcgaag aagaaaccaa gtttgcccca atttggcaga
     4561 atgtcgcccc cgaattgaaa caacgtttcg agaaagacca cgcccgactg cggcagatga
     4621 tggcaagccc cgaatacggt aacgcggcgt ggaataccgc ttttgccaca accctgcgcg
     4681 accacgcgcg ctttgaagaa cgcgagctgt ttcccgccgc cgaaccgttt ttgccggcat
     4741 gattccgttt tgcggcaaat atattaatga taaacaagga acacacatga aatttaccaa
     4801 gcaccccgtc tgggcaatgg cgttccgccc gttttattcg ctggcggctc tgtacggcgc
     4861 attgtccgta ttgctgtggg gtttcggcta cacgggaacg cacgagctgt ccggtttcta
     4921 ttggcacgcg catgagatga tttggggtta tgccggactg gtcgtcgtcg ccttcctgct
     4981 gaccgccgtc gccacttgga cggggcagcc gcccacgcgg ggcggcgttc tggtcggctt
     5041 gactatcttt tggctggctg cgcggattgc cgcctttatc ccgggttggg gtgcgtcggc
     5101 aagcggcata ctcggtacgc tgtttttctg gtacggcgcg gtgtgcatgg ctttgcccgt
     5161 tatccgttca cagaatcaac gcaactatgt tgccgtgttc gcgctgttcg tcttgggcgg
     5221 cacgcatgcg gcgttccacg tccagctgca caacggcaac ctaggcggac tcttgagcgg
     5281 attacagtcg ggcttggtga tggtgtcggg ttttatcggt ctgattggta cgcggattat
     5341 ttcgtttttt acgtccaaac ggttgaatgt gccgcagatt cccagtccga aatgggtggc
     5401 gcaggcttcg ctgtggctgc ccatgctgac cgccatgctg atggcgcacg gcgtgatgcc
     5461 ttggctgtcg gcggctttcg cgtttgcggc aggcgtgatc tttaccgtgc aggtgtaccg
     5521 ctggtggtat aagcctgtgt tgaaagagcc gatgctgtgg attctgtttg ccggctatct
     5581 gtttaccgga ttggggctga ttgcggtcgg cgcgtcttat ttcaaacccg ctttcctcaa
     5641 tctgggtgtg catctgatcg gggtcggcgg tatcggcgtg ctgactttgg gcatgatggc
     5701 gcgtaccgcg ctcggtcata cgggcaatcc gatttatccg ccgcccaaag ccgttcccgt
     5761 tgcgttttgg ctgatgatgg cggcaaccgc cgtccgtatg gttgccgtat tttcttccgg
     5821 cactgcctac acgcacagca tacgcacctc ttcggttttg tttgcactcg cgcttttggt
     5881 gtatgcgtgg aagtatattc cttggctgat tcgtccgcgt tcggacggca ggcccggttg
     5941 agacaaaccg ccgcagattt cgggtctggg cggtttgctt ttcagacggc agggcggtca
     6001 gttgccgtcc agccagcggt cgcgtgtggt tttggcttct tcaaaatagc ggtacagggc
     6061 ttcgcggtcg tcggtggtca ggatgtttgc caaaacgtcc aactgtttgc ccaagccttg
     6121 aaccagttgc agcaggctgt ctttgttggc aaggcagatg tccgcccaca cggcgggatg
     6181 gccggaggcg atgcgggtga agtcccgaaa gcccgtggcg gcgaatttca gatattcctg
     6241 tccgtcgggg tggtcgagaa tctggtggac ataggcgaag gcggtcaggt ggggcatatg
     6301 ggagacggcg gcgaaaaccg cgtcgtggcg ttgcgcgtcc atcgtataaa tttccgcacc
     6361 gaccgcgtgc cacagttctt ctaccaaggc aatgccgtct gaatgttcgc cgccgtgtgg
     6421 cgtgatgatg agttttctgt ggcggaacag cccgaactgc gcggcttgcg caccgcttct
     6481 gtccgaaccg gcaatcgggt gggcggcgat gcagtggtgc agacggtcgg gcagacagcg
     6541 gcggaaggct tcgatgaccg aagatttggt gctgccgaca tcggaaatcc aagtgtgttc
     6601 cggcaaaacg gggcgcagcg cggtcaaaat ggcgggaacg gtggcgacgg gcgtggcaat
     6661 cagtaccaag tccgcaccgc cgatgctgtc cgcgtcgatg gcaacggaag cccggtcaat
     6721 cacgccgcgt tccaatgcac gttcgaggtt gtcgcggtcg gtgtcgatac cggtaacggt
     6781 gcggacgagt ccctgccttt tgaggtcgag aacgaacgaa ccgccgatca gccctacacc
     6841 gatgagggca atatggttca aaatgggcat ttgtgtaaac ggttttcgca aagtaccgtc
     6901 atggtagcct atcggcggaa tatgccgcaa gttcggcagg aaaaaggaga agaaatggac
     6961 aaaatcagag ttgccgccgt gcagatggtg tcgggcgtgt cgccggaaac caacgtcgct
     7021 gccatgaaac gcctggtcgc acgggcggcg gagcagggtg tggattgggt gctgctgccc
     7081 gaatattggg tgctgatggg cgcaaacgat accgacaaac tcgcgcttgc cgagcctttg
     7141 ggcggcggac gctttcagac ggcattgagc gaaacggcga aagaatgcgg cgtggtgctg
     7201 ttcggcggaa ccgtgccgct gcaaagcccc gaggcgggca aggtcatgaa tactttattg
     7261 gtgtacgggc gcgacggcaa acggacgggg ctgtaccaca aaatgcacct cttcggcttt
     7321 tccggtttgg gcgaacgcta tgccgaagcc gataccatcc gcgcgggcgg ggatgtgccg
     7381 cacttgtcgg cagaaggcgt gtcggtggcg gcgggcattt gttacgatgt ccgctttccc
     7441 gaatttttcc gccgccagct gccgtttgac gtattgatgc tgcccgctgc gtttacgcac
     7501 acgacgggca aggcgcattg ggagctgctg ctgcgcgcgc gtgccgtcga aaaccaatgt
     7561 tacgtcgtgg cggcggcaca gggcggtttg cacgaaaacg gacggcgcac gttcggacac
     7621 agcatgattg tcgatccgtg gggcgacgta ttggacgtat tacccgaagg cgaaggcatc
     7681 gttacggcag acatcgatgc caaccgcctg aacagcgtcc gcaaccgcct gcccgccttg
     7741 aaataccggg ttttggatgc cgtctgaagg ttcagacggc atcggtgccg gggaatcaga
     7801 agtggtagcg catgcccaat gagacttcgt gggttttgaa gcgggtgttt tccaagcgtc
     7861 cccagttgtg gtagcggtat ccggtgtcca gggtcagctt gggcgtgatg tcgaaaccaa
     7921 caccggcgat gacaccaaga cccaagctgc tgatgctgtg gctttcgtga taggcagatt
     7981 tggggacaaa acctgttggg acaggagctc cctgtgcagc gccttgcgtt ggtttagagg
     8041 taacaatcgt gtttttgctt tccactgaat gaacctgatg tttaacgtgt ccgtaggcga
     8101 cgcgcgcgcc gatatagggt ttgaatttat cgaatttatc gttgagtttg aaatcgtaaa
     8161 tggcggataa gccgagagaa gaagcggcgt ggaagctgcc gtttccctga tgttttgttt
     8221 gggtttcttt gtagttgttg tttatctctt cagtaacttt tttagtagaa gaattacttt
     8281 ctttccattt tctgtaactg gcataatctg ccgctattct ccagccgccg aaatcgtagc
     8341 cgaccgacac tcgggggtgg atggaatgcg tacggatgtt tctgaaataa tcgcttactg
     8401 tgcttatttt gtctttgtct gcaccggttg gttccggata attgtgggta atgcgttcgg
     8461 cggcgtaggc taaatccgcc tgcacataat acgggctgcg gctgccgtct tcacttgccg
     8521 cctgcgctgc ggaagagaag agaaga
//
LOCUS       DAWFJR010000081         8296 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWFJR010000081 DAWFJR010000000
VERSION     DAWFJR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.081"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(54..1310)
                     /locus_tag="WGR04_001660"
     CDS             complement(54..1310)
                     /locus_tag="WGR04_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ0795179.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTSISQIKDLLDEEETAFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQPAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDTRITRLAGKADHVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSVLPEENELQVERFLSEHPEFEPVNCAELLQSLKVDL
                     DTGKYLRLDSAKHQTDGFFAAVLQRK"
     gene            complement(1345..2199)
                     /locus_tag="WGR04_001661"
     CDS             complement(1345..2199)
                     /locus_tag="WGR04_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ0795180.1"
                     /translation="MNYALDALWWKLTDPTVRDLASLLTAPPLWQSGCELGVRELLGK
                     RGFRYLLALDADPAPLTEHLARHAPFGHRLGIYAEELSAFWFANAPHAELLAHNLTVS
                     GSDGSTQGAADFVARLNGKPCHIELTCKYYGGTGKPEDMRGLDPKDTLLGKAAKLSAQ
                     LALFRTSDGIRTLRRHGLPLDVKPVSIVRGIGFFPQGFNAFEPPLNPYGWRGIYIQDW
                     AEYGFERREARYHLLDRMAYLAPARVAETETLNETEIRRIDNGLIAVLECRPDGFWHE
                     IERIMKAV"
     gene            complement(2270..3088)
                     /locus_tag="WGR04_001662"
     CDS             complement(2270..3088)
                     /locus_tag="WGR04_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0795181.1"
                     /translation="MTAPKPELPDYLGNIRIVLTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFNPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVPELLQAANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTDSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTLQQRLKDGQS"
     gene            3354..4139
                     /locus_tag="WGR04_001663"
     CDS             3354..4139
                     /locus_tag="WGR04_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ0795182.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDGWLESGDVVAANPKVLAQMLKIISAHV"
     gene            4409..6528
                     /locus_tag="WGR04_001664"
                     /pseudo
     CDS             4409..6528
                     /locus_tag="WGR04_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            6652..8202
                     /locus_tag="WGR04_001665"
     CDS             6652..8202
                     /locus_tag="WGR04_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ0795183.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQEAGFL
                     TVESHQYERGWFTSTETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAYIETEFKYAPETEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNGYDAPLFKIKLADKGDAAFEKVHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFIDSEGQFRFDTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTLPSGKIDVGGKIMFK
                     DLKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT         2013 a         2049 c         2310 g         1924 t
ORIGIN      
        1 ataattgatg aaatgccgtc tgaacgcttt gaaaagggtt cagacggcca gggttattta
       61 cgttgcaata cggcggcgaa gaagccgtcg gtttggtgct ttgccgaatc gaggcgcagg
      121 tatttgccag tatccaaatc gaccttcagg ctttgcagca gttcggcgca gttgacgggt
      181 tcaaattcgg gatgttcgga caggaagcgt tcgacttgca gttcgttttc ttccggcaac
      241 acgctgcaag tggcgtaaac caagcggcct tgaggtttta ccagtttgga ggcggcatcg
      301 aggatgctgt gttgctgttc caaaaggttg gcgacggtct cagcagattg gcggtatttg
      361 aggtcgggat tgcggcgcag tgtgcctaag ccggagcagg gcgcatccac taaaacgtgg
      421 tcggctttgc ctgccagtcg ggtgatacgg gtatcatgtt cgctgctgat gcgttcgggg
      481 tggatattgg tcagtccggc gcgggtcata cgcggtttga ggttggcaag gcgtttttcg
      541 gcgatgtcga aggcgtagat tctgccttta ttggccattt gcgcaccgac agccaaggtt
      601 ttgccgccgg caccggcaca gaaatcgaca atgatttcgc cgcgttttgc gcccaccaat
      661 aaagcaagca gctggctgcc ttcgtcttgg acttccagtg tgccgtctaa aaacagttcg
      721 tgtttgttaa gcgcgatttt gtttttcagg cggatgcccc aaggcgaata aggcgtggcc
      781 tctgcatcgg cactttcggc ttgcaacagc ggcagcactt tatcgcgttt gcctttcaaa
      841 gtgttgacgc ggatgtcgag cggggcaggc tggttgatgc tgcggccgaa ggcgaggatt
      901 tcttcttcgc gccaatgctg ttgcagctgt tccaccagcc attgcggcaa ttctgcggcg
      961 gtattcaggc cgtctgaaaa ctcggtttta cgggctttca aattgccgag gaacgctgtt
     1021 tcttcttcat caagcaggtc tttgatttgg ctgatgctgg tgcttctgcc gagaaccagt
     1081 gcggcgagtg cggctttgcg tggctgcgcg tgcggacggc gcagggcggt actgattttt
     1141 tgatagtggc gcagcgcggc aaaggcggtt tcggcgattt cgtggcggtc ttggcggccg
     1201 agttttttgt gttcgcggaa ataggcggag aggacggcat cggcaggctg tttgaaagtc
     1261 agcatttcag ccaaaacttt ggcggtatgg tcgagttgtg cggcgttcat cattaatggg
     1321 ttctcttaaa gttagtaaat ggtttcagac ggccttcata atgcgttcga tttcgtgcca
     1381 aaaaccgtcc ggccgacatt ccaaaacggc aatcaaacca ttgtcgatgc ggcggatttc
     1441 ggtttcgttc aatgtttcgg tttcggcaac acgcgcaggc gcgagatagg ccatgcggtc
     1501 gagcaggtga tagcgcgctt ctcggcgttc aaacccgtat tccgcccaat cttgaatata
     1561 gatgccgcgc cagccgtatg ggttaagcgg cggctcaaag gcgttgaaac cttgcggaaa
     1621 aaatccgatg ccgcgcacga tggaaacggg ttttacgtca agcggcaaac cgtgccgccg
     1681 caaggttcgg atgccgtctg aagtgcggaa cagggcaagt tgggcagaca gcttggcggc
     1741 ttttcccaac agcgtgtctt tggggtcgag tccgcgcatg tcttcgggtt tgcccgtgcc
     1801 gccgtaatat ttgcaggtca gttcgatatg gcagggcttg ccgttaagtc ttgccacaaa
     1861 atccgccgcg ccttgcgtgc tgccgtccga accggaaacc gtgaggttgt gcgcaagcag
     1921 ttcggcgtgc ggcgcgttgg caaaccaaaa tgccgacagt tcttcggcgt aaatgccgag
     1981 gcggtgtccg aatggggcgt gtcgggcgag gtgttccgtc agcggcgcgg gatcggcatc
     2041 caatgccaaa agatagcgga aaccgcgttt ccccaatagt tcgcgcacgc ccaattcgca
     2101 accgctttgc cacaaaggcg gcgcagtcag cagcgaggct aaatcccgca cggtagggtc
     2161 ggtcagtttc caccacaggg catcgagggc gtagttcatg gttttcctgt ttctgtattc
     2221 tgatttttcc tgaaatgccg tctgaaaaac ggattcagac ggcatgcgct taggattgcc
     2281 cgtccttcag acgctgttgc agcgtattga aaaaaccgcg caggatgtcg atgtcttcgg
     2341 tttgcgtgtt ggcgcgtccg aacaggctct gcatacggcg catcaggcgc tcgccgttgc
     2401 ggcggttgaa aaagccgatg tcgttcatca cgctttccat gtgggcgacc atgcctttga
     2461 tttgctcgtg ggtcgcagcg tggtcttctt gttgaagatg ggtcatgggc gaatcggttt
     2521 gactgaagat ttcgtagcac acgacctgca cggcttgggc gaggttgagc gagaaatagt
     2581 cgggattgcc gttgatggtc atcagtcggt tgcaggcttg gacttcttcg atgctcaagc
     2641 cgaaagtctc gttgccgaaa accagtgcca ctttctcgcc tcggttggcg gcctgcagta
     2701 attcgggcac caaatcgcgc ggggtttgca gcggcgcggt aatttcgcgg cggcggctgg
     2761 tcagggcgca ggcgatggtg gtgtcggcaa gggcttcgtc caaagaggcg gcaatggtgg
     2821 cattttccaa aacatctgcc gcaccggaag cgaggatgaa gctttcttcc ggtaatttaa
     2881 acgattgcgg atgctccgga ttgaacacgg gcggattctc cgtcatcggc gtagccatca
     2941 gattgggggc gacgatggtc agtttgtgca ggcccattgt tttcatcgcg cgcgcggcag
     3001 agccgatatt ggcgggatgg ctggtgcggg tcaggacgat gcggatgttg ccgagatagt
     3061 cgggcaattc gggtttaggg gcagtcatgt tcggttttcg gtcggaatgg aatagggtat
     3121 aatgcgccgt ctttttcgtt cggacggtca aatgccgtct gaaatgttct ttaacaacgt
     3181 ctgaaatgtt ctttaacaac gtcggaaatt taacgttgcg cctgaagcag acacgcgtca
     3241 ggcggtattt tacccgattt ggcaggattt accccaacca aataaactcc gattttgccg
     3301 ctccgaagga ctgtacgtcc gaaccggcgg tatgccaccc ttaaggaaat ctgatgaatc
     3361 cgtttttgaa tacagccttt aaagccgccc gccgtgccgg tcagatgatg atacgcgccg
     3421 cgggcaatct cgatgccgtc aaaaccgaca gcaaagcctt caacgatttt gtttccgatg
     3481 ttgaccgcaa ttccgaaatc atcctggttg aggctttgaa agaagcctat ccgcaccaca
     3541 aaatcacttg tgaagaaagc ggctcccacg gcaaagccgc agccgagtac gaatggatta
     3601 tcgatccgct cgacggcacg accaatttcc ttcacggtca tccccaatac gccatctcta
     3661 tggcgctgct gcacaaaggc gtgttgcaag aagctttggt ttacgccccc gaacgcaacg
     3721 acgtatatat ggcttcgcgt ggcaaaggcg cgttgctcaa cgaccgccgc atccgcgttt
     3781 ccaaccgcat cgaactgaac cgctgcctga ttggtaccgg cttccctgtt gtcgatcaaa
     3841 gcatgatgga caagtatctg gcgattttga aagatttctt ggccaaaacc gccggcggcc
     3901 gtcgtgaagg tgcggcttct ttggatttgt gcgccgtagc aacaggacgt tttgacggct
     3961 ttttcgagtt taacctcaaa ccgtgggaca ttgccgccgg tgcattgatt gttcaggaag
     4021 cgggcggtat cgtaaccgat atgtcgggtg aagacggctg gctggaaagc ggcgatgttg
     4081 tcgctgccaa tcccaaagtg ctggcgcaaa tgttgaaaat catttccgca cacgtttaaa
     4141 tttcagcccg ctcccgggaa tgcgggaaaa cgggcgggta tgccgtctga agtatcgcgc
     4201 cgatatttca gacggcattt ttactggcag cgggggcagg aaggtggaaa cggctaaatg
     4261 cccgaaactg ccgaagatgg aaacccatat ctgattaaac ggagaaccct gtacgatttg
     4321 tttatagtgg attaacaaaa accagtacgg tgttgcctcg ccttagctca aagagaacga
     4381 ttctctaagg tgctgaagca ccaagtgaat gggcaaagcc aacgccttta ccgcaccgat
     4441 taccgtcgtc aattacgacg aacaagccct caataacacc gaagcgcgta ctttggtgga
     4501 tgccgtagcg aaaaaagacg cttccgtctg gcagttcggc ggcgaaagca acacgctgac
     4561 cggactgtat ttcagaggtt atcagcttga ctcgcgccaa ttcagcgtca acggtttggc
     4621 aggtatgtac ggcacgcaag gcacggccag cgtgcaagtt ggatcggcac aactgattaa
     4681 aggcgcatcc actgccgtaa acggcatgga ccctgaaggt gcggtatccg gttccgtcaa
     4741 tatcgaaacc aaaaaagccg ccgatgaagg caaccgcaaa atcggtttgg gctggttcag
     4801 taacaaccgc gcccaaggta cgttcgactt gggccaacgt ttcggcgaaa acaaagcatt
     4861 cggcgtgcgc gccaacggca aactgcgcca cggcgacacc ccgcgccacg gttacagcga
     4921 agacaacaaa gaatttgcgg taaatgccga ttatcgcggc gaaacactgc gcgtagcgtt
     4981 cgattccatc tacgcaaaac gcaaaaccaa cggcggccgc gcgcgtatgc aggatattca
     5041 aaacgccggc ggacgcttgt ttggcgcgcc cgatggtaaa accaacctgc tgccttcttg
     5101 gaactggcaa aataccgccg gccaaaccaa tatgcttacg tttgaatggg atgcgtttga
     5161 taatgcacag attacaggcg gtatcggcta caacaaggca cgctattacg gcacgctgat
     5221 ttccccgacc gtatgtttga atgctaccag cacttgtacc gatatgcaaa acacaaacgc
     5281 cagcggcaaa gtaacggcac gcaactatga ctacaatacc ggtactgcgc gcctgaccga
     5341 ccaatatttc cgcacattga gtatgaacct gaccgcacgc ggcgaatttg aaaccggccc
     5401 ggtaacacat aactggagta tggctttcga ccgcgttatc cgccagcgga aaaccataaa
     5461 cggcagcggg aacggcaaca gcaagataga agtgaaggca aacgaaaata ttgcgaatca
     5521 attggcatcg tttacagccg actatcccaa ttcttgggag aactctgcca acttggatgc
     5581 caatatcaaa gtcaacagcc tggctttgtc cgacaccttg ggcttcgtgg ataacaaata
     5641 ccgcctgact ttgggcggac gtttccaaac agtcgaatat accgacaaaa aatcacagag
     5701 cggcgatgcc aaacgtttca gcccgatgct gatggcggcg tgggtgccgc aacctgattt
     5761 ggttgtgtac ggcaactata tggaagactt ggagcctgcc gacatcaaaa ccgatgattc
     5821 cggcgaaacg acaatggcga aaccgcgtgt cagccgccag tttgaagtgg gcgtgcgtaa
     5881 aaactggggc aatttcgtaa ccacgttaaa cgcgttccaa atcaaacgcc cggctactgg
     5941 cgcggtcata caactaccaa gacaacaaaa ggggtaacaa caacgctgac ctatggcaac
     6001 aattccgatt tcgcccgcta taaggcgcaa ggcggcgtag ccggcgacga gcaaggtatg
     6061 gaacgcaatc gcggtatcga gttcaatgcc tatgctaacc tgttaaacaa aaccctgcgt
     6121 ccgactttgg gcctgatgta tctgcaatcg accgtgaaag agtatccaaa ttcacgcgat
     6181 atgctggtta acggcgtaca agtcgccaat ccgcgcgtaa ttgccaaagc aggtttggaa
     6241 tgggatacac cgtttgccaa aggattgact ttaaacggta atgtttcgta tttcggcaag
     6301 tcttaccaag acacgcaaaa acaatacgcc ttcccgtcct ataccttgat tgacgtaggc
     6361 gcgcgctaca aaaccaagct cggcaaaaat actttgaccg tcagcagctc ggtggaaaac
     6421 ctgttcaaca aaaactattg gcaggtacag cgcggccaat acgaccgcag cttcgccgtc
     6481 gtcggtatgc cgcgcactta ctggctgaag gcggaattgg atttctgatt tccctatatc
     6541 cggaaaatgc cgtccgaaca ggtttcagac ggcatttctt ttaaagagat atggcgggat
     6601 acgcttaaat aatgctattc tagcgtcttt gaaacctgct tgtttgaagt gatgaaaaaa
     6661 cctttgattt cggttgcggc agcattgctc ggcgttgctt tgggcacgcc ttattatttg
     6721 ggtgtcaaag ccgaagaaag cttgacgcag cagcaaaaaa tattgcagga agcgggcttc
     6781 ttgaccgtcg aatcgcacca atatgagcgc ggctggttta cctctacgga aacgacggtc
     6841 atccgcttga aaccggagtt gctgaataat gcccggaaat acctgccgga caacctgaaa
     6901 acagtgttgg aacagccggt tacgctggta aaccatatca cgcacggccc tttcgccggc
     6961 ggattcggca cgcaggcgta cattgaaacc gagttcaaat acgcgcctga aacggaaaaa
     7021 gttctggaac gcttttttgg aaaacaagtc ccggtttccc ttgccaatac cgtttatttt
     7081 aacggcagcg gtaaaatgga agtcagtgtt cccgccttcg attatgaaga gctgtcgggc
     7141 atcaggctgc actgggaagg attgacggga aaaacggttt atcaaaaagg tttcaaaagc
     7201 taccggaacg gctatgatgc ccccttgttt aaaatcaagc tggcagacaa aggtgatgcc
     7261 gcgtttgaaa aagtgcattt cgattcggaa acttcagacg gcatcaaccc gcttgctttg
     7321 ggcagcagca atctgacctt ggaaaaattt tccttagaat ggaaagaggg tgtcgattac
     7381 aacgtcaaat tgaacgaact ggtcaatctt gttaccgatt tgcagattgg cgcgtttatc
     7441 aatcccaacg gcagcatcgc accttccaaa atcgaagtcg gcaaactggc tttttcaacc
     7501 aagaccgggg aatcaggcgc gtttatcgac agcgaagggc agttccgttt tgacacgctg
     7561 gtttacggcg atgaaaaata cggcccgctg gacatccata tcgccgccga acatctcgat
     7621 gcttctgcct taaccgtatt gaaacgtaag tttgcacaaa tttccgccaa aaaaatgacc
     7681 gaggaacaaa tccgcaatga tttgattgcc gccgtcaaag gcgaggcttc cggactgttc
     7741 accaacaatc ccgtattgga cattaaaact tttcgtttca cgctgccgtc aggaaaaatc
     7801 gatgtgggcg gaaaaatcat gtttaaagat ttgaagaagg aagatttgaa ccaattgggt
     7861 ttgatgctga agaaaaccga agccgacatc agaatgagta ttcctcaaaa aatgttggaa
     7921 gacttggcgg tcagtcaggc aggcaatatt ttcagcgtca atgccgaaga tgaggcggaa
     7981 ggcagggcaa gtcttgacga catcaacgag accttgcgcc tgatggtgga cagtacggtt
     8041 cagagtatgg caagggaaaa atatctgact ttgaacggcg accagattga tactgccatt
     8101 tctctgaaaa acaatcagtt gaaattgaac ggtaaaacgt tgcaaaacga accggagccg
     8161 gattttgatg aaggcggtat ggtttcagag ccgcagcagt aatgggtcaa atgccgtctg
     8221 aaatgttcag gcggcatttt gttgtattgg aaactgccgt tttcaaccat tcctttagat
     8281 aagccgtgtc ggattc
//
LOCUS       DAWFJR010000082         8266 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWFJR010000082 DAWFJR010000000
VERSION     DAWFJR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8266
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.082"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            323..1255
                     /gene="pip"
                     /locus_tag="WGR04_001666"
     CDS             323..1255
                     /gene="pip"
                     /locus_tag="WGR04_001666"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ0795184.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGNRAILNNIGKIRHIPTVIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLVDALVQAVEDILPHLL"
     gene            complement(1312..2508)
                     /gene="bamC"
                     /locus_tag="WGR04_001667"
     CDS             complement(1312..2508)
                     /gene="bamC"
                     /locus_tag="WGR04_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ0795185.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTLIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGASDIFFAHK
                     AMKEVYGDKNKDTTMWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(2517..3392)
                     /locus_tag="WGR04_001668"
     CDS             complement(2517..3392)
                     /locus_tag="WGR04_001668"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ0795186.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQVDIALARELNNRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            3698..5089
                     /locus_tag="WGR04_001669"
     CDS             3698..5089
                     /locus_tag="WGR04_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ0795187.1"
                     /translation="MSGQLGKGADAPDLVYGLEDNPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLIFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGKEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            complement(5151..5903)
                     /gene="rlmB"
                     /locus_tag="WGR04_001670"
     CDS             complement(5151..5903)
                     /gene="rlmB"
                     /locus_tag="WGR04_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ0795188.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYIQEGKSDARTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGDADLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(5970..6458)
                     /locus_tag="WGR04_001671"
     CDS             complement(5970..6458)
                     /locus_tag="WGR04_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ0795189.1"
                     /translation="MTALLVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIERRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            complement(6463..7182)
                     /locus_tag="WGR04_001672"
     CDS             complement(6463..7182)
                     /locus_tag="WGR04_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEZ0795190.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLDGTPPQEMAQAHKARLLAELKNHPPVAAFPPQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIKRVIYGAVEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRSVLSCFFQNKRKG"
     gene            complement(7186..8127)
                     /gene="miaA"
                     /locus_tag="WGR04_001673"
     CDS             complement(7186..8127)
                     /gene="miaA"
                     /locus_tag="WGR04_001673"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ0795191.1"
                     /translation="MPIPKAFALLGPTACGKTALALKIAETLPVEIISLDSALVYTGM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKCPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIERALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
BASE COUNT         1525 a         1933 c         2689 g         2119 t
ORIGIN      
        1 cgggaatgac ggttcgggca ttccttaaat cacccgtgta tcgctgtaaa tcttagagat
       61 ggcggaatat agcggattaa caaaaaccgg tacagcgttg cctcgcctta gctcaaagag
      121 aacgattctc taaggtgctg aagcaccaag tgagtcggtt ccgtactatc cgtactgtct
      181 gcggctcgcc gccttgtcct gatttttgtt aattcactat atcgcgattt ttcggcattt
      241 gcctttcggg gcggcttgtg tctcgtgcgt gatgttgcgt gtgggaatgt tcggattgtc
      301 agaagcaata tgggagaaga tgatgtatga gataaaacag ccttttcata gcggatactt
      361 gcaggtgtct gaaattcatc aaatttattg ggaagagtcg ggtaatcccg acggtgtgcc
      421 ggtcattttt ttacatggtg ggccgggcgc gggggcttcg cctgaatgtc ggggtttttt
      481 caatcccgat gtgttccgca tcgtcatcat cgaccagcgc ggttgcggac gttcgcgccc
      541 gtatgcttgt gcggaagaca atacgacttg ggatttggtg gcagatattg aaaaagtccg
      601 tgaaatgctg ggtatcggga aatggctggt gttcggcggt tcgtggggca gcactttgtc
      661 gctggcttat gcccaaaccc atccggaacg ggtaaaggga ttggtgttgc gcgggatatt
      721 tttgtgcagg ccgtctgaaa cggcgtggct gaacgaggcg ggcggtgtga gccggattta
      781 tccggaacaa tggcaaaaat ttgtcgcgcc gattgctgaa aatcggcgga accggctgat
      841 tgaggcgtat cacggattgc tgtttcatca agatgaagaa gtgtgcctgt ctgccgcaaa
      901 ggcttgggcg gattgggaaa gctatctgat ccgtttcgag ccggaggaag tggatgaaga
      961 tgcttatgcc tcgctggcaa tcgcgcgttt ggaaaaccat tattttgtca acggcggttg
     1021 gttgcagggc aatagggcga ttttgaacaa tatcggcaaa atacggcata tcccgaccgt
     1081 tatcgtacag gggcggtatg atttgtgtac gccgatgcag agtgcgtggg cgctgtcgaa
     1141 agcctttccc gaagcggaat tgagggtggt tcaggcaggg catcgtgcgt tcgatccgcc
     1201 tttggtggat gcgttggttc aggcggttga ggatattttg ccccatttgt tgtaaaaagt
     1261 tccgcataaa aaagcagctt ctgtttggaa gctgcttttg ttttgaatgg tttaacgcag
     1321 ttcggaatgg agtttgccca ataatgcgga tgcgtctttg ccggcatatg cgctgccgtc
     1381 tttgttgagc aggacgatgc gcgagccgtt ggcgacaggc tcggcataga caatcagttc
     1441 cggctgttcg gcaggtttct ccgctttgcc tttgcccagc aggcgtttga acaggccggg
     1501 tttttgttcg gtaactgcat tgctttcgtt cggggctttt tgaaccagga aagcgtggcg
     1561 ttcggtgttt tgaccgacga cggtcagccc gatgcggtcg agggcgagcg cggtgcgccg
     1621 ccagtttctg ccgtagtcgc caaagacaat caggcttttg ccttcgatac gcgccatttc
     1681 gttggcggcg ggaagggtag gtttttttgc cgatgcgttt tccgcctgct gtccgtcaac
     1741 gcccaaatat tgcataaagc gcgtcaggaa agcggcttca agattgggat cggacgggga
     1801 gggctgccac atggtcgtgt ctttgttttt gtcgccatac acttctttca tcgctttgtg
     1861 ggcgaagaag atgtcggaag cgccgttttt gccctgttcg atacggacga tgaatttgtc
     1921 gcgctcgccg gtggagtaga tgccgcccaa accgactgtg tcgaataggc ggcgcaagct
     1981 gtcttggggg attttggcgc ggttttccgc ccactcggtt tccatttgtc cgatggcagg
     2041 ttcttcggac ttgatgtcga agccgttttc ctgccagaag gctttcagga gcggccatat
     2101 ttccgccggt gatttgccat cgacaacgag ccagcgttgg ctgccgtcgc gctcgaggcg
     2161 gacacctttg acgcttttca atacctcggc atcggcaggc tgttggacgg cgggtgtgcg
     2221 gcgtttttcc aaatcgctgg cgcggacggc gcccgaaccg gcaggcaggc ggtagaggtt
     2281 gccttggtcg ggattgttca aatcgggcgg gacttcgagt ttgatcaggc ggtgcgaccg
     2341 gctttggtag tcgagcttgg gctgttcggt tttgctgccg gagcaggcgg caagcccgat
     2401 gagtgcgagc gcggcaatga cgggtttgat aagggtcatc gtgtcatcct gtgtgatgga
     2461 tattaaagtg tttgttgcgt tatgccgtcc gaacggttca gacggcatag ttatatttag
     2521 agttgtcctg aggctttcag ggcggcgcgg acttttgcct gtccgccttc cgtcagcgga
     2581 acgagcggca ggcggacgtg cggttcgcat ctgcccaagg cggataccgc ccatttcggc
     2641 gcggcggggc tgggttcgca gaacatggtg tcgtaaatcg gaatcagccg gttgttgagt
     2701 tcgcgtgcga gggcaatgtc gacttgcagc gcggcgcggc acatatcggc aaagagcttg
     2761 ggcgcggcgt tggcggctac ggtaatcacg ccgtgtccgc cgcacagcat gaacggcagg
     2821 gcggtatggt cgtcgccgga aaggacgacg aagccttcgg gcgcgcggtt gatgagttcg
     2881 atgttgctgc cgatgttgcc gctggcttct ttcacgccga cgatgttggg gatttcggca
     2941 aggcgcagga tggtttcgtt gttcatgctg acgacggttc tgccgggtac gttgtagagt
     3001 atcatcggaa tcgcggcggc ttcggcaacg gctttgaaat ggcggtacat gccttcttgc
     3061 gaaggtttgt tgtaataggg gacgacggag agggtgtagt ccgccccggc tttttcggcg
     3121 gcttgggaaa gggcgatggc ttcgacggtg ttgtttgccc ctgtgccggc gatgacgggg
     3181 acgcgtttgg caacgtgttt gacgacggct tcgatgacag cggtgtgttc ttcgacggag
     3241 agggtggcgg attcgcctgt cgtgccgacg gcaacgatgc cgtccgtgcc gttttcaatg
     3301 tgccagtcga ttaagtcgcg gagttgttcg taatggatgc tgccgtcttg attcatcggg
     3361 gtaatcaggg caaccaagct accttgtaac atacagaacc ttttatcagt tgtggtgtag
     3421 ggggcggtaa tgcttctgat tgtagcctac tttaccgcag gtgtgaaatc cggcgggtcc
     3481 gggatgagcg gggcggcttg acggcggatg cgtcgggttt gtatttttgt gtttgaaatc
     3541 aggttattgc gttttgtaaa cgatattccg gcggcgacgg ttcggaatct gtttgccgga
     3601 cgggcgcgta tgccgtccga aatcgaaatt gaagcggtat ggcggattcg ctacaatccg
     3661 cgcttggatt tttcaacctt taaaataagg aaatacaatg agtggtcagt tgggcaaagg
     3721 ggcggatgcg cctgatttgg tgtacggttt ggaagataat ccgccgtttg ggaatgcgct
     3781 cttgagtgcc gttacccatc ttttggcgat ttttgtgccg atgattacgc ccgcgctgat
     3841 tgtgggcggc gcgctggaac tgccggtgga aatgacggcg tatctcgtgt cgatggcgat
     3901 ggttgcgtcg ggtgtcggca cttatttgca ggtcaaccgc ttcgggccgg tcggttcggg
     3961 gatgctgtcc atccagtcgg tgaatttctc gttcgttacc gtcatgattg cgctcggcgc
     4021 ggggatgaag gagggcggtt tgactaagga tgcgatgatt tcgacgctgt tgggcgtatc
     4081 gtttgtcggc gcgtttttgg tgtgtttctc ggcgtggctt ctgccgtatt tgaaaaaagt
     4141 gattacgccg acggtcagtg gcgtagtggt gatgctgatc ggcttgagtt tggtacacgt
     4201 cggtattacc gatttcggcg gcggcttcgg cgcgaaggcg gacggcacgt tcggttcgat
     4261 ggaaaacctg gggctggcgt cgctggtgtt gctgattgtg ttgatattca actgcatgaa
     4321 aaacccgctg ttgcggatga gcggcattgc ggtcggtctg attgccggct atatcgtcgc
     4381 actgtttttg ggcaaggtgg atttttcggc actgcaaaac ctgccgctgg ttacgctgcc
     4441 cgtaccgttt aaatacggtt ttgctttcga ctggcacgca tttattgtgg cgggcgcgat
     4501 tttcctgttg agcgtgtttg aggcggtcgg cgatttgacc gcgacggcaa tggtgtccga
     4561 ccagccgatt gaaggcaagg aatacaccaa acgcctgcgc ggcggcgtgt tggctgacgg
     4621 cttggtgtcg gtgattgcga cggctttggg ttcgctgccg ctgacgacgt ttgcgcaaaa
     4681 caacggcgtg attcagatga ccggcgtggc ttcgcgccat gtgggcaaat atattgccgt
     4741 gattttggtg ctgttgggtt tgttccccgt tgtcggacgc gcgtttacga cgattccgag
     4801 tccggtgttg ggcggcgcga tggttttgat gttcggcttg attgcgattg cgggcgtgcg
     4861 gattttagtc agccatggca tccgcaggcg cgaggcggtg attgcggcaa cgtcggtcgg
     4921 cttgggcttg ggtgtcgcgt ttgagccgga agtgtttaaa aacctgcccg tcttgttcca
     4981 aaactctatt tccgccggcg gcattacggc agtcttgctg aatttggtct tgcccgaaga
     5041 taaaaccgag gcggcggtca agtttgatac cgaccacttg gaacactgat tttgaaaatg
     5101 aatgccgtct gaaacggaat ccctgtttca gacggcattg tttttgaggc ttacgctttt
     5161 tcgtttttta atacgcgttg tcggcgtgtt tcacttaata ccattccggc agacacggag
     5221 acgttcatgc tttcgaccgt gccgaacatg ggtatggaca ccagcatgtc gcaatgttcg
     5281 cgtgtcaggc ggcgcatacc gtcgccttcg ttgcccatca cccatgccgc gctgtcgggc
     5341 agatcgcaat ggtaaaggtc ggcatcgccg cccatatcgg tgccgatgat ccaaatgccg
     5401 tattctttca gctcgcgcag ggtgcgggcg aggttggtta cagtgatgta ggggacggtt
     5461 tccgccgcac cgcaggcgac tttgctgacg gtggcgttca gccccgcgct tttgtctttc
     5521 ggtgcgatga cggcgtgtac gcccattgcg tcggcggtac gcaggcacgc gccgaggttg
     5581 tgcggatcgg tgatgccgtc gagtatcagc agcagcggcg gttcgctgag gttttccaat
     5641 acgtcttcga ggtggacgtg gtttttggag gcatcgataa atccgaccac gccctgatgg
     5701 cgcgcgcctt tgctgatggc gttgaggcgg tcggcatcgg caaaatatac gcggatgttt
     5761 tcgtttgccg ccttttccaa cacttcgcgc gtgcgtgcgt ctgatttgcc ttcttggatg
     5821 tagagttcga cgatggattt ggggttttgc cacaatcggg cgttgacggc gtggaagccg
     5881 tagatgggtc tttggtttgc catgatggtg ctttgtaaaa agggttcaga cagcattata
     5941 gcaatttgcc ggtatgccgt ctgaaagggt taaaacaggt aggcgatgta tttcaccaac
     6001 aggataaaca agatggatac ggcgcagccg attttgaacg ccgtgccgac gacaagcccc
     6061 aacagcgtac ccaagcccgc tttacctgcc tgaagcatat tgcgccgttc gatcagttcg
     6121 cctgccgccg cgccgataaa gggaccgagt attagtccgg gaagggagaa aaatatgccg
     6181 atgatgctgc cggccaatgc gccgcgaacg gcgagcttgc ccgctccggt atattttgtc
     6241 ccccatatgc ctgccacata gtccgccagt atgccggcaa ggctgatgag tccgaccgtc
     6301 cacaaaacgc ccgcgccgta gatttggtag ccgccggcat aggcaagcag ccatgttccg
     6361 gcaaacatca atgccaatcc gggcagggcg gggtaaacga tgcccgccgt accgacggct
     6421 atcagggcga gggcgaggat gacgagcaag gcggtcatag gttcaacctt ttcttttgtt
     6481 ctgaaaaaaa cagcttaata cggagcggca ttcttcttgc aggattccgc cccgtatggc
     6541 ggtgtgcgta ttgaggcgtt tgtcggcaaa caggttgacg atgctgcctg ctgcgccagt
     6601 tttgggttct accgcaccgt atatcacgcg cttgattcgt gcctgtatca gtgcggacgc
     6661 gcacatggcg cagggttcga gggtgatata tatgtcgcat ccgtcaaggc ggtagttttg
     6721 catttcgctg cctgcctgtg ccaaggcgtt gatttcggcg tgtcggctga cattgcagtc
     6781 ggcaatgcag gtgttgtgtg ccgatgcgat gattttgccg tcggaaacga tgactgcccc
     6841 gacgggtatt tcgccgtcgg cggcggattg ttctgcttgg cgcagtgctt cgcgcatgaa
     6901 gtgttccatt tcttcctgcg gcggaaaggc ggcgacgggc ggatggtttt tcaattcggc
     6961 aagaaggcgg gctttatgcg cttgcgccat ttcttgcggc ggtgtgccgt ccagcagcga
     7021 ctcgagttgc cacagcgtgc ttttcgtgag ggtcaaaccc gatgctttga gcagcagaaa
     7081 ggctttgacc gaaccgtttt gccgcagttc ttcgagcgta cggataccga gcctgtgcag
     7141 ggcggcgacg gttttggggg cgagcggcgg tgtggtcagc atggtttatg cgccgaaaaa
     7201 ccgttttgcc gcctcaatca ggcgtgtgca tgaagtgccg tctgaaaacg ggtcggcaac
     7261 gcagtctaaa ggtgttttgc gcaaccaagt cagttggcgt ttggcaagtt ggcgggtggc
     7321 ggcaatgcct ttctcgacaa atgccgggaa atcggttttt ccgtccagat attcccatgc
     7381 ctgacggtag ccgacgcagc ggatggcggg ggagtcggcg gtcaggccgg gatagcggcg
     7441 gcgcaggttt tctacttcgc cgataaagcc ctgttcaagc atcaggtgga aacgcagggc
     7501 gatgttttca tgcaggcggg cgcggttttc gggaatcagg gcggcggtat gcaaatcgaa
     7561 ggggagcgta tgggggcttt gtctgccgag gtgttcgctc atcggtttgc cggtcagata
     7621 ataaacttcc aaagcgcgtt cgatacgctg gctgtcgttc ggtttcagac ggcatgcggt
     7681 ttcagggtcg actttttgca gggtgcggta gaggaaatcc aagccgtaca tctgtttttg
     7741 ttcgtccaag tcggcacgca ggcaggcatc ggcctcgggc aaatcgttca aaccttgggt
     7801 cagggcgcgg aaatacatca ttgtgccgcc gacaatcagc ggacatttcc cacgcgccgt
     7861 gatttcgccg atcagacgcg tgcagtcttc gacaaaacgg gcggcactgt atgtctgaac
     7921 aggcgtaatg atgtcgataa ggtggtgcgg gacaaaggcg cgttcggagg cggacggttt
     7981 tgccgtgccg atgtccatac cggtatagac tagcgcggaa tcgaggctga tgatttcgac
     8041 aggcagggtt tcggcaattt tgagggcgag cgcggttttg ccgcaggcgg tcgggcctaa
     8101 gagggcaaag gctttgggaa tcggcatgat gtttcagggt tgcgaaaatg cggattatag
     8161 cggaaagcgt gccgacgtta tattttggtt tgcggaagca cgccgacggc aagggggcgt
     8221 gtttaccgta tgcctttata tagtggatta acaaaaacca gtacgg
//
LOCUS       DAWFJR010000083         8223 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWFJR010000083 DAWFJR010000000
VERSION     DAWFJR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8223
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.083"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(208..1593)
                     /gene="pntB"
                     /locus_tag="WGR04_001674"
     CDS             complement(208..1593)
                     /gene="pntB"
                     /locus_tag="WGR04_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ0795192.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIAPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALGVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TSSSGSQEIGEYREVKAADVAEMLKSANSVIITPGYGMAVAQAQYPVAEITELLHKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1659..2042)
                     /locus_tag="WGR04_001675"
     CDS             complement(1659..2042)
                     /locus_tag="WGR04_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ0795193.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLARTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(2046..3587)
                     /locus_tag="WGR04_001676"
     CDS             complement(2046..3587)
                     /locus_tag="WGR04_001676"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ0795194.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKATVWACPLIYKVNAPSEDELPLLKEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVSLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFFVTRRMLN
                     MFRKG"
     gene            3877..4710
                     /gene="serB"
                     /locus_tag="WGR04_001677"
     CDS             3877..4710
                     /gene="serB"
                     /locus_tag="WGR04_001677"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ0795195.1"
                     /translation="MPHALVLQFPSAEALPSDFPSRLPEPDCADEKRMRFIVEEGFSL
                     SGADAALLGSRQIDHAVLPDMNFDELGLIVSDMDSTLITIECVDEIAEGVGLKNKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPSAEFLLDECKRHNVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANILEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGVGVAYRAKPKAQAAADACINFGGLERVRGLF
                     R"
     gene            4774..5997
                     /locus_tag="WGR04_001678"
     CDS             4774..5997
                     /locus_tag="WGR04_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ0795196.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGADGVY
                     TSFREGVAQASGMRRVAVLTLCGAVAGVGWCLLKALGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEDEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGAAAVFFQRTAQKFPFIKRDNIKIIPLAVCMFVLIGAIAVWFP
                     EILGNGKAGNQLTFSGLTGWQYALELAAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPAMSSESAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            6158..7738
                     /gene="purH"
                     /locus_tag="WGR04_001679"
     CDS             6158..7738
                     /gene="purH"
                     /locus_tag="WGR04_001679"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ0795197.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQTLTKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLGEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGEPQIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDVYPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTDVRHFRH"
BASE COUNT         1818 a         2227 c         2339 g         1839 t
ORIGIN      
        1 taaaaatcca tcttcaacac ggagatggat tttgtttgtt tcgttgattt tgtgtcagtt
       61 tcagatgtag gtgcttattc ggacgggccg tctgaaaatg tttgccccaa tgcaaaaaaa
      121 atcactgcaa accttcataa acggggtttg cagtgatttt ttcaaatcaa acagattgaa
      181 aacctgcgcc gaattgttca gacggcatta ttttttcagt tcggacagaa tgtcatctac
      241 ggttttcttc gcatctccga aacacattac gctgttttcg ttgaagaaca gtgggttttg
      301 tacacctgcg tagccggtat tcatcgagcg tttgaagacg acgacttctt ttgccttcca
      361 tacttccaac acgggcatac ccgcaatcgg gctgttcggg tcggtttggg cggcaggatt
      421 gacggtatcg ttcgcaccga tgaccaagac cacatcggtt tcggggaaat cgtcgttgat
      481 ttcgtccatt tccaacacga tgtcgtaggg gactttggct tcggcaagca gtacgttcat
      541 atgaccgggc aggcggccgg cgacggggtg gatgccgaag cgtacttctg tgccgttttt
      601 atgcaaaagc tcggtgattt cggcaacggg gtattgcgcc tgtgcgactg ccatgccgta
      661 gcccggggtg atgatgacgc tgtttgcgct tttcagcatt tcggcaacat cggcagcttt
      721 gacttctcgg tattccccta tctcttggct gccggaagat gatgtgccgc tgtcgctgcc
      781 gaaaccgccg gcaattaccg agacaaacga gcggttcatg gctttgcaca taatgtagga
      841 cagaatcgcg ccgcttgagc caaccagcgc gccggtaacg atgagcaggt cgttggagag
      901 cataaagcct gccgctgcgg ccgcccagcc ggagtaggag ttgagcatgg acacgaccac
      961 gggcatatct gcgccgccga tggaggcaac caagtgccag ccgaatgcga gggcaatcag
     1021 ggtcataatc agcaggatga agccgctgcc gtcaatgccg acgaatacga gcagcaacac
     1081 aaacgatacg ccaagtgcca gtgcgttgag cttgtgtttg gcgggcagtt gcagcgggct
     1141 gctgctgatt ttgccgttga gcttgccgaa tgcgaccagc gagccggtaa aggttaccgc
     1201 gccgatgaaa atgcccaaat agacttcgac cagatggatg gtgtgcatat cgtgcgaaac
     1261 gttgcccggc gcgatatagc tgttgaagcc gaccaaaacc gccgctaggc cgacgaagct
     1321 gtgcagcagg gcaatcagtt cgggcatttc ggtcatttcc acctttttgg ctttgtagat
     1381 gccgattgcc gcgccgatga gcatggcgat gatgatccag cccagtccgt gggtattgtc
     1441 ggaaaaaaca gttacaaaaa gggcgaccgc cataccggcg ataccggaat agcagccctg
     1501 tttggcggtt tcctgtttgg acagccccgc cagtgagaag atgaataaaa ttgcggcaac
     1561 gatatacgcc gctgttacga gtcctgaaga catggaaatt ctccgatttt cgatgatttg
     1621 tttttaatgc cgtctgaact gttcagacgg catctgtttt aagcagccgc gacaaacagc
     1681 ccgacggtgc aggcaaatcc gcccgcccac atcagcgagc gcaatgctgc tttgtctgcg
     1741 atgtagcacc agataaaggc gaggcggaac aggatgaaca ggccggcaag cgtgttgacg
     1801 gttgcttgtc cggcattgcc ggttgcgtgt gccgtcaaaa cggcggcggc aaacggtgca
     1861 aaggcttcaa aaccgttttg ctgcgcggcg tgggcacggg cggctgtgcc ttgcgtgcgc
     1921 gccagaaaat cgcgcggatt gtggttgtct ttaaaccgga atccgcccgc ttttttggca
     1981 tatgccgcac aaaaaagcgg caataagcag gcaatcagga tgcaccaata ggcaaaagtc
     2041 atgctttatc ctttcctaaa catattcaac atgcggcgcg ttacgaagaa gccgccgaag
     2101 atattgatgc tggcaatcag gatggcgatg aaggcaagca gggtaacgaa accgttgcct
     2161 tggctgattt gcagcagcgc gccgacgacg atgatgccgg agatggcgtt ggttaccgac
     2221 atcagcggtg tgtgcagcga gtggctgacg ttccaaacga catagtagcc gatgacgcag
     2281 gcaaggacga acacgataaa gtggttcagg aatgctgcgg gtgcgaccgc gccgacccac
     2341 agcaccaata cggcggcgat gacggcgggc gcgagttttt tccacaggga aacaggtttc
     2401 ggctcgggct tggcggcagg cgcggctttt tcagacggcg tttgctgcgg ctgggcggaa
     2461 acttgaatcg gcggaggcgg gaaggtgatt tcgccgtcgt gggtaacggt catgttgcgg
     2521 ataatcacgt cttcgaagtc caacgtgatt tcgccgtctt tgttcgggct taacagcttg
     2581 gtcaggttga ccaagttggt ggcgtaaagc tgggaagact gtccggcaag gcggtttgcc
     2641 atgtcggtgt agccgatgat tttcacgccg ttgccggtta cggacaattc gcccgggcgg
     2701 gtgagttcgc agttgccgcc cgtcgccgcc gccaaatcga cgatgacgga gccggatttc
     2761 atgctttcca ccatttcttt ggtaatcagc ttgggcgcgg gtttgcccgg aatggcggcg
     2821 gtggtgatga tgatgtccac ttctttcgcc tgctcggcaa agagcttcat ctcggctgcg
     2881 ataaattcgt cgctcatcac tttggcgtag ccgtctccgc tgccgcccga ttcttgtggg
     2941 aagtcgagtt tcaggaactt gccgcccatc gattcgattt gttccgccac ttccaagcgg
     3001 gtatcgaacg cgcgtaccac tgcgccgagc gagtttgccg taccgatcgc cgccaaacct
     3061 gccacacctg caccaatcac caaaacctgc gcgggcggca ctttgccggc ggcggtaatt
     3121 tgaccggtga agaaacggcc gaaggcgttg gcggcttcaa ttacggcgcg gtagccgctg
     3181 atgtttgcca tcgaagacaa agcgtccaaa gcctgcgcgc gcgaaatacg gggcaccatg
     3241 tccattgcca gcgcgttgac tttcttggcg cgcaaggctt cgaccaaagc ctcgttttgg
     3301 cgcggccaca ggaagctgac gatggtctgt ccttctttga gcagcggcag ctcgtcttcg
     3361 gacggcgcgt taaccttata aatcaaaggg catgcccaaa ccgttgcttt gtcggcaacg
     3421 gtcgcgcctg ctgtttggta agcggcatcg tccaaacttg ccgccaaacc tgcaccgctt
     3481 tcgacaacgg tttcaaagcc cagtttgccc agcagggcaa cggtggcggg cgtgcaggcg
     3541 acgcgggttt cgccggataa tgactcacgt gggataccga ttttcatctc tgaatccttt
     3601 ttcgggttgt ttatatgtat cgtgggttaa atttaaatcg tggcggggcg gagcaacgcc
     3661 gtaccggttt aaaaccgatt cacttcaaat gttaatatat tttagataat ccccttataa
     3721 cgaattttca tcaggctggc aatagttgcg gcattttccc gtgttgtccg acacatactg
     3781 ccgtttgcgt ttgaagtcat tgtccgattg cagtccctcc cgccgtaaca ttctttgccg
     3841 tttcactata taatccgcat tttttgagcc gccgttatgc cgcacgccct cgtcctccaa
     3901 tttccctccg ccgaagccct gccttccgac ttcccctccc gcctgcccga acctgattgc
     3961 gccgatgaaa agcgtatgcg ttttatcgtt gaagaagggt tttctttaag cggggcggac
     4021 gcggcgttgc ttggcagccg tcaaatcgac cacgccgtgt tgccagatat gaattttgac
     4081 gaactcggtt taattgtcag cgatatggat tcgacactga ttaccatcga atgcgtcgat
     4141 gaaattgcgg aaggcgtggg tttaaaaaac aaagtagcgg aaattaccga gcgttcgatg
     4201 cgcggcgaac tcgatttcga acagtcttta cgcagccgcg tcgcgctgtt ggcgggattg
     4261 gacgaacaga ttttggcgga cgtttatgaa aacgttttga agctctcgcc cagtgcggaa
     4321 tttttgttgg acgaatgcaa aaggcacaat gtgaaattcc tgctggtgtc cggcgggttc
     4381 acgtttttta ccgaaaggct gcaacaacgc ctcggcttcg aataccaaca cgccaatatt
     4441 ttggaaattg aaaacggcag gctgaccgga cgtttgaaag gcagaattat cgacgcgcag
     4501 gcaaaggcgg atttattgcg cgaataccgc agccgcctcg gattgcagcc gcatcaggtg
     4561 ttggcgatgg gcgacggtgc gaacgatatt ccgatgctca aagaagcggg cgtgggcgtg
     4621 gcttaccgcg ccaaaccgaa agcgcaggca gccgccgatg cctgtatcaa tttcggcggt
     4681 ttggagcgtg tacgcggcct gttcaggtag gcggatagga aacggatgcc gtctgaaagg
     4741 tgttcagacg gcatttgaac ggcaggaacg acagtgggac gcagaaaact ttggtttgcc
     4801 ctggcagcag cgggcgttat cggcggtttg gtcggcattg tgctgacgga actgatgcac
     4861 ttcatacagc atacggcata cggttatggc gcggacggcg tgtacacttc gttccgcgaa
     4921 ggtgtggcac aggcttccgg tatgcggcgc gttgccgtgc tgacgctgtg cggcgcggtc
     4981 gcaggagtcg gctggtgctt gctgaaagcc ttgggcaagc cgcaaatcga aatcaaagcc
     5041 gccttgaagc agccgttgca ggggctgccg tttctgacga cggtttttca tgttctgctg
     5101 caaatcataa cggtcggact cggttcgccg ctcggacggg aagtcgcgcc gcgtgaaatg
     5161 actgccgcgt ttgcttttgc cggcggcagg cggttgggtt tggatgaaga cgaaatgcgc
     5221 ctcttgattg cctgtgcgtc cggcgcgggt ttggcagccg tgtataatgt gccgctcgcc
     5281 tccacacttt tcatcctcga agccatgctg ggcgtgtgga cgcagcaggc ggttgccgcc
     5341 gcgttgctga cttccgtcat tgccaccgcc gtggcgcgta tcggcttagg cgacgtacag
     5401 cagtatcatc ccgccaacct tgccgtaaat acttcattgc tttggttttc cgccgtcatc
     5461 ggcccgatat tgggcgcagc cgccgtcttt ttccagcgta ccgcccaaaa gttccccttt
     5521 atcaagcgcg acaacatcaa aattattccc ttggcagtct gcatgtttgt gctcatcggc
     5581 gcgattgccg tgtggtttcc cgaaattttg ggcaacggca aagcaggcaa ccaactgact
     5641 tttagtggtt tgaccggttg gcaatacgct ttggaacttg cagccgtcaa atggctggtc
     5701 gtcttgatgg cgctggcggc aggggcatac ggcggcctga ttacgccgtc tatgatgctc
     5761 ggcagcacca tttcttttgc agccgccgcc gcgtggaaca gcgttttccc cgcaatgtcg
     5821 tctgaaagcg cggcagtcgt cggagcggcg gtgtttctcg gcgtgtcgct caaaatgccg
     5881 ctgaccgcca ttgtgttcgt attggaactg acctacgcgc cgctgtccct gctgctgcct
     5941 ttgtgcatcg gcatggcggg ggcattggga acatcgggaa aaatgggctt caaatgaatg
     6001 ccgacaaatg ccgtctgaaa ggttttcaga cggcatttct ttgtaaattg tcagcaatat
     6061 ctgcaaaaac ctactgccaa aaaactagaa atagcggata ataccgcccc gaaaaagttc
     6121 ccctttcccc atccaactgt cagaaaagga agccccaatg tccgtcatca aacgcgccct
     6181 gatcagcctg tccgacaagg caggcgcggt cgaatttgcc caaaccctga ccaagctcgg
     6241 tgttgaaatc ctctctaccg gcggtacagc aaagctcttg gctgatgcag gcgttcccgt
     6301 tatcgaagtt gccgactata ccggcttccc cgaaatgctc gatggccgcg tgaaaaccct
     6361 gcatccgaaa atccacggcg gcatcctcgg tcgtcgcgat ttgggcgaac acgtcgccaa
     6421 gatggaagaa cacggtatcg gcaacatcga cctcgtgtgc gtcaaccttt accccttcgc
     6481 cgccaccatc gccaaaccaa actgcacact ggaagacgcg attgaaaaca tcgacatcgg
     6541 cggcccgact atggtgcgtt ctgccgcgaa aaactggaaa cacgtcgcca tcgttaccga
     6601 taccgccgat ttccctgcca tcgccgccga actcgaagcc aacaacggcg cattgagcga
     6661 caaaacccgt ttcaacctct cacgcaaagc attcagccat accgcccaat acgacggcat
     6721 gatttccaac tacctgacct cgctttcaga cgacgtcttg agcggagagc cccaaatcgg
     6781 cgaattcccg agccagttca accaaagctg gattaaagtg caagatatgc gctacggcga
     6841 gaatccgcac caacgcgccg cgttctaccg cgatgtgtac cccgccgcag gcagcctttc
     6901 cgcctacaaa cagctccaag gtaaagaatt gtcgtacaac aacatcgccg atgccgatgc
     6961 cgcatgggaa gccgtcaaat cctttgacgc gcccgcctgc gtgattgtga agcacgccaa
     7021 tccgtgcggc gtcgccgttg cagccgatac cttgaccgcc tacaaactcg cctacgccac
     7081 cgacaccacc agcgcgttcg gcggcatcat cgccttcaac cgcgaagtgg acggcgaaac
     7141 cgtcaaacag attacagaca accagtttat ggaagtcctg atggcgccga agttcaccgc
     7201 cgaagccctc gaaatcgccg ccgccaagaa aaacgtgcgc gtgttggaag tgcctttgga
     7261 agcaggcgcg aaccgctttg aactcaaacg cgtcggcggc ggattgttgg tacaaacgcc
     7321 cgacatccac cgcatcagcc gcgccgattt gaaagtcgtc tccaaacgcc aaccgaccga
     7381 acaggaatgg aacgatttgc tgttcgtttg gaacgtcgca aaatacgtca aatccaacgc
     7441 catcgtcttc ggcaaaggcg gtcaaaccta cggcatcggc gcaggccaaa tgagccgcgt
     7501 ggacagtacc cgcatcgccg cccgcaaagc gcaggacgca ggcttggact taaacggcgc
     7561 atgtgccgcc tctgatgctt tcttcccctt ccgcgacggc gtggacgtga ttgccgagca
     7621 gggcatcaaa gccatcatcc atccggcagg ttcgatgcgc gatcaggaag tcttcgacgc
     7681 agcggacgaa cacggcatcg ccatggtcgt aaccgacgtg cgccacttcc gccattaacg
     7741 cagatgaaca aggcaatgcc gtctgaaggg ctttcagacg gcattttgcg ctattttgtg
     7801 aaggtaggga tgacggttcg ggtattcctg acagggtgga ttttcaaggt gttgtatagg
     7861 gtgtaggagg attcgtaaaa ggtgggatgc agggtgtgct tcagcccgct gcatcaaaaa
     7921 tttttggaga atcggcggga gtcggcggtt ttggtttcgg cggggacggt ggaaatgggt
     7981 aacattgacg gaatcgacgg aagcggtggg cttcagccca ccgcttgtat attggaatca
     8041 ccgtatcata gcgccacccg cgcccaccca aggcacacaa ccgttgcgta gctcagggag
     8101 cggcagggca acccatcgac acaaccggac agttgccgga caacacaacc gaatgcaagg
     8161 caggttgatg atgagtaccc gataccatta cgcaggtata gtgaattaac aaaaaccagt
     8221 acg
//
LOCUS       DAWFJR010000084         8160 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWFJR010000084 DAWFJR010000000
VERSION     DAWFJR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.084"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            235..447
                     /locus_tag="WGR04_001680"
     CDS             235..447
                     /locus_tag="WGR04_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ0795198.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILESVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            595..2178
                     /locus_tag="WGR04_001681"
     CDS             595..2178
                     /locus_tag="WGR04_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ0795199.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLKPAAAAKAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTIDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EQGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLGGGGKLPAIQKILNHNTYYVAHEYMEIFNDPIYVNGFIEWANRHRLAYIGDTDLH
                     VSFVSWMAEHTRERILALAGDDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEPFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKQFFEDHKTYVPARFTDYVAAFVEHGAEAFVQ
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2260..3357
                     /locus_tag="WGR04_001682"
     CDS             2260..3357
                     /locus_tag="WGR04_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ0795200.1"
                     /translation="MNHTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQDTQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKVGGQVLFGFNATDGAFEVVPQVFSWQLSFTDNIGVVQQNFLDLMAECRVEHV
                     YLLHNALGEQENIPSYAEALKREGHNPFFSA"
     gene            3471..4586
                     /locus_tag="WGR04_001683"
     CDS             3471..4586
                     /locus_tag="WGR04_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ0795201.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     ACGQALLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIIGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4711..5166
                     /locus_tag="WGR04_001684"
     CDS             4711..5166
                     /locus_tag="WGR04_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ0795202.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5237..5713
                     /locus_tag="WGR04_001685"
     CDS             5237..5713
                     /locus_tag="WGR04_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ0795203.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQQ"
     gene            5758..6051
                     /locus_tag="WGR04_001686"
     CDS             5758..6051
                     /locus_tag="WGR04_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEZ0795204.1"
                     /translation="MKPSLLLQSRKKEILAVFGKYPLICNPRVFGSVSRGDDTENSDI
                     DLLVDAKTGTTLLDLGGLQEELQKLLGIKVDLLTPDDISAHFRDKVLTEAVAL"
     gene            6048..6320
                     /locus_tag="WGR04_001687"
     CDS             6048..6320
                     /locus_tag="WGR04_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEZ0795205.1"
                     /translation="MKMQKELSVYLKQILQAAQYIRLYTDKMDYGQFSADTKTVQAVV
                     FNLFLIGENATHILKSYPEFAEETKYLNWIGMRGYCAIGLPMATLK"
     gene            6503..7420
                     /locus_tag="WGR04_001688"
     CDS             6503..7420
                     /locus_tag="WGR04_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ0795206.1"
                     /translation="MKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEFGEI
                     GIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAHAAS
                     VIDELKAKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLAASKTDLCA
                     PEQLAEFHDKAAKLFPPKAKVVEVRNAQLDIRWLDIPVIEKSRYRLKALPDNTMGFQS
                     QGFTFPARRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGANQV
                     SWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            7485..8042
                     /locus_tag="WGR04_001689"
     CDS             7485..8042
                     /locus_tag="WGR04_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0795207.1"
                     /translation="MKLKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDDTVFHRVIDGFVIQGGGFTEDLVQKATDKAVANESGNGLKN
                     TAGTIAMARTADPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
BASE COUNT         2174 a         1926 c         2112 g         1948 t
ORIGIN      
        1 caacgacgta ccggtttttg ttaatccact ataaatgcgc cgattgccaa ggcaacgatg
       61 ctcaaccagg gtttttgcga agcgttcatt gttgttgtcc tgatggaaat tgccgccatt
      121 atgcgaaagc gtccgtattt tttcaaaccg ccgcaccgtt tccgcgtttg cccaagttca
      181 cgctccgact tacaataacg cccgtcagaa tgcagtacca acgaaaggaa aatgatggaa
      241 acccttatcc tcgacatcgg cggtatgagc tgcggcggct gcgtcaaaag cgttacccgg
      301 atactggaaa gcgtcaaagg cgtggcaagc gtcgaagtca gccttgaaaa caaaagtgcg
      361 accgtcggtt acgaccccgc gcaaaccgat gcaggggcgt tgattgaagc cgttgaagac
      421 ggcggctatg atgccgcgtt gaaataaagc ggcaaaaatg ccgtctgaag ccttcgcgcc
      481 tcctcaatgc cgtctgaagt acgctccgcg ccttcagatg gcatttgatt tagttgaacg
      541 ggtaggatga attggttaaa cttgcgggca atagtgcctt cagacaaaca aaatatgccg
      601 aacaaaaaga aaaaagccgc ctttgcgcgt gctgcaacgg agaacggtgt gccggacgtg
      661 tccaacatca aaaattcata tgatgatttg atgtatgaat ccaaagcatt ttcccaaacc
      721 gccatcaacg ccttggaggc tcgcgcccgc atgatgggtc tgaagcctgc agcagcggca
      781 aaagccaagg tcttggagct gggctgttcg atgggcggca atatcatcac gcaggctctt
      841 tattacccag atgcggaatt tgtcggtatc gacttgtccg gcaggcaggt tgcgcagggc
      901 aacgccatca ttgaaaaaat gggcttgaaa aatgtgcgtc tggaagaaaa agatattttg
      961 accatcgatg agtcattcgg gaagttcgac tatatcatcg tccacggcat ttggtcgtgg
     1021 gtgcctgacg cagttaaaga caaaattttt tcgatttgcc ggaacaacct gaccgaacag
     1081 ggcattgcct atatttcata caatgtttac ccgggctgga agcggcagga gcagttgcgc
     1141 gaaattatgt actttgccgg cagggatgtg cttgaagaac ccttggaagc gcgaacgcgg
     1201 aaaggcttgg acgcgctcaa ggcgctggcg gaaattttgg aaaacgacaa gggcttgggc
     1261 ggcggcggca aacttccggc gattcaaaaa atattgaatc acaacactta ttatgtcgca
     1321 cacgaatata tggaaatatt taacgacccg atttacgtca acggcttcat cgaatgggca
     1381 aaccgccacc ggctggcgta tatcggggat accgatttgc acgtgtcttt tgtttcttgg
     1441 atggcggagc atacgcggga gcggattttg gcattggcag gggatgatta tattgccaaa
     1501 gaattttata gcgatatttt atccgaccgc caattccgcc gttcgctttt atgccgtgag
     1561 gaagtcgggg atactgtcag gcgtgacgag tcggttgccg tcgaagtgat agaaagtttg
     1621 aatttccgcc cggcaagagg ggaaacaatc aactttgatg aaaacgatac cctgctttcg
     1681 ggcatacgcg atgtgatgaa aaccggagag ccgtttaaaa cggaagatgt tgcggaaaat
     1741 ctcgcccgcc gatttcccgg tctggaattt gaccgcatga aaatcaattc ccagctttta
     1801 ttgcaaacca ttctcgggcg tttttctgtt tcatcagaca atgcgggcaa acaatttttt
     1861 gaagaccata aaacctatgt gccggcgcgt tttacagatt atgtcgccgc ctttgtggaa
     1921 cacggcgcgg aagcatttgt ccagcctgcc aaccgttaca acgaaagcac cccctcattc
     1981 gggtacgggc atttgtacat tatgcggcaa ttgtcgcggc cgacgagcaa acaggcattg
     2041 attgaaacgg ttgccgaaaa cttgaacatc gtcagcacca cgcccgacgg tttgacattc
     2101 catccgcctg ccgaagtgta tgtggaagaa atattggcag acttggcaga caggcatttt
     2161 ctcgtttcgg cggattgacg ggcaagtccc gacggcaatc cgtccgatat attttcagac
     2221 aggaaagaca cacactgcga aaagcggtac acgacacata tgaaccatac atttaccttg
     2281 cccgatacgc gcccgtatcc ccaaaatccg attaaaaacc acctgctgct caatgcctac
     2341 cagttggcgc acaattcttc ccaggcttcg cgcaaactct cgtccggcca acttcaaacc
     2401 gaaatcaggg ggatgcttga gcaaaaccac tatatcaacc tttccctcgc gctgacgatg
     2461 tcgcccgatg ccggaactta tgccgcgctg ctttccagtg tgaacgcggt actcgattgc
     2521 gagaaagaag gcgaagtgca gtggttcgcc ctgccggtcg tgctggtgtc cggctgcaag
     2581 aaagaacggg caatcgagat gaagctgccg acggaggcat tgtttgcctg tttgcaaaac
     2641 tatccgcacc tgcgcgcgtt gacccaggat acgcaatggc tgccttatct tgtgcattcg
     2701 tccgatttaa gtgcggtcgc gccggatgag tggtggcgtg ccaaacaaaa taccgaagcg
     2761 gcagcgcaac acttacgccg tttcgccccg cgccctttgc tgttgcccga agggcagtcc
     2821 gtccacgtcg tttacgcgct gggtttcggt agcggcaaag ttcagacggc attgggtcag
     2881 aaccttcttc aggcaggtct gcccttgatg caggtatggc aggaaaatct tgcatcggaa
     2941 ggcgttacgt tgtttgccaa cccgctttcc ccggattctc cggtacgcgc gctttcagac
     3001 ggcagccaca cgcgccagcg tatggcgatg gatgtgtttg cggcaaacgc gatacgcgcc
     3061 gtccgtatgc agagtccgcg cgtcggcgtg gtcgctgcgg caaaggtggg cgggcaggtt
     3121 ttatttgggt ttaatgcgac cgacggcgcg tttgaagtcg tgccgcaggt gttttcgtgg
     3181 cagctttcct ttaccgacaa tattggcgtt gttcagcaaa attttttgga tttaatggca
     3241 gaatgccgcg tggaacacgt ttacctgttg cataatgcct tgggcgaaca ggaaaacatc
     3301 ccgagctatg cggaagcgtt gaaacgggaa gggcacaatc cgtttttcag cgcatagtga
     3361 tttgtagtag tgataggctt tctcatttct ataataaatc aaaataaaga gcacaacact
     3421 ttttcattct gtgttgtgcc ttgagtgaaa cgaaaggatg aattataaat atgaagattg
     3481 taattgcacc ggattcgttt aaggaaagct tgacagctca acaggtagct gaagcaataa
     3541 aaagaggctt ccaacaatcg atagcagatg tggaatgtct cctctgtcct gtcggtgatg
     3601 ggggagaagg cactgtagat gctatccgac attctcttga cctagaagaa aaatgtctcc
     3661 aagtgacagg accttttgga caaaaagaag tcatgcgcta ttttcaaaaa gaacaactag
     3721 ccctattcga agttgctgat ttggttggtc ttggaaaaat cccgctagag aaacgaaatc
     3781 cattacaaat ccaaactcgt ggtattggag aattgattcg ccacctcatt agtcaagaaa
     3841 ttaaagaaat ctatattggc gttggcggta cggccagtaa tgatggaggt attgggattg
     3901 ctgctggttt aggttatcaa ttttatgatg aggatggaaa taccttgccc gcttgcggtc
     3961 aagccttact aaacctagct tctgtttcaa cagaaaatcg ctatgaaatt cctgaagatg
     4021 ttcacattcg tattttagca gatgttgtga gtcccttatg tggacaccaa ggtgcgacct
     4081 atacgtttgg caagcaaaaa gggttggatt ctactatgtt tgaggccgta gatcaggcaa
     4141 tacaagattt ttatgaaaaa gtctcccctg caacattaaa acttaaagga gcaggagctg
     4201 gtggaggcat cgctggtggt ttgtgcgcct ttgctcaggc aagtatcgta tctggaattg
     4261 acacctgcct ggacttgatt gactttgata agaaagtgtc agatgttgac ttggttatca
     4321 ttggtgaagg aagactagat cgtcaaagtt tagcagggaa agcgcctatt ggtgtagcaa
     4381 aaagaacccc tgtcggagtt cctgttgtcg ctatttgtgg cagtctcgtt gaagatttgc
     4441 cttccctgcc atttgaaaat atccaagctg ccttttctat tttggagaaa agtgaacctt
     4501 tagaagatag tttgaaaaat gccagtctct atttggagca tacggcttct aatattggac
     4561 acttattaaa tatgcctaag atttagccaa accatttctt ccagatggat gttttggctg
     4621 gttctgcctt atgcggttac gattaaccga ccgatgccgt ctgaaagggt ttccgcttca
     4681 gacggcatcg cgccggaaca gggaggggat atgaaaaaac cgaaaatcct ttttgtctgc
     4741 ctcggcaaca tctgccgttc gccgatggcg gaatacattt tgcgccgccg cgccgccgaa
     4801 gcgggcattc cccttgaagc ggacagcgcg gggacatcgg gctggcacga cggcgaagat
     4861 atgcaccgcg agacggcaaa gatattgaaa aaacacggta tcgatgcttc aggctttacc
     4921 agccgcaaaa tccgccaaag cgatgcggcg gcgtttgact gcatcatcgc gatggacggc
     4981 aagaatttgt ccgaattgga aagaaccttc ggcaggcggc cggaaaaaat attcaagctg
     5041 accgacctga tacccgaaag cggttacgac catgtccccg atccgtggta tacgggcgat
     5101 tttgaagaaa ccttcaggct tgcggatgcg ggctgtcggg cattgttgga aaagatttcc
     5161 aaataaagca attgaataca aatataaaac cgcctcctgc cgtatcggtt gttcagacgg
     5221 cataaacaga gaatttatga aaacaaattt caaacagaaa attatcgaac aggcacgcag
     5281 cgaaggcttg caggtaaccg ctttgcgcga gcaggtatta gatattgtct tgcagcaaag
     5341 cggcgtgatt aaagcctaca acgtcttgtc gcagatgcag cagcaaagcg agggcgtgct
     5401 tgcgccgccg accgcctacc gcgcccttga tttttgggcg gagcagggcg ttttgcacaa
     5461 agtggcggcg gtcaacggct atattttgtg cagccacgcg cagcacgagt gcgacgacca
     5521 ttgccacgac cacgaagaag ccgaagcgca ccacagcgcg tttattttgg tctgcaccga
     5581 atgcggcacg gcggacgagc aaaccctcag ccacgagtgg gcggcactgc gcgcaggcgt
     5641 tgccgaaagc ggctttgcgc tgaaagaaga acacgttgtt ttaaccggga tttgtaaaaa
     5701 atgtcagcag taatcggtgg tttgcattga taataagcct ttcgaaagga gcagataatg
     5761 aaaccgtctc ttttgttgca aagcagaaag aaagaaatat tggcagtttt cgggaaatat
     5821 ccattgattt gcaatccaag ggtattcggt tcggtttctc gcggagatga tacggaaaac
     5881 agcgacattg atttgttggt ggatgcaaaa acagggacaa cattattgga tttgggcggg
     5941 ctgcaagagg aattgcagaa actcttgggc ataaaggtcg atttgctgac acccgatgac
     6001 atctcggccc actttagaga taaggtgttg actgaggctg tcgcattatg aaaatgcaga
     6061 aagaattgtc ggtctatttg aaacagatat tgcaggcagc gcaatatatc cggctatata
     6121 ccgacaaaat ggattatggg caattttctg ccgacacaaa gactgtgcag gcagtcgttt
     6181 ttaatttgtt ccttatcggt gaaaacgcaa cccatatcct taaatcgtat ccggaatttg
     6241 ccgaggaaac caaatatttg aattggatag ggatgagggg gtattgcgca ataggattgc
     6301 ccatggctac tttgaaatga acctgtctgt cgtttgggag acggttttga acgtcatacc
     6361 ggcgatgtca gccgatatat cgaatttgct ggaacaattg tcaattgatg aagaacatga
     6421 taacagctgc catcactttt aacattattt aatcatttaa aacaccctat cttcaaattt
     6481 taaagtggga catcacatag aaatgaaaaa aaccaaagtc cacctgattt caggtttttt
     6541 gggaacaggc aaaaccactg cgctcaaaag cctgatggcg caaaaagacc cgaacgaaaa
     6601 atgggtcatc atcgtcaacg agttcggcga aatcggcatt gacggcgcgg tattgagcga
     6661 caacggcatc cctgtggcag aaatcgccgg cggctgtttg tgttgcaccg ccggcccgca
     6721 aatgggcgta accgtgcaga aaatgctgcg cgatgccaag cccgaccgcc tgatgattga
     6781 ggcaagcgga ctggcgcacg cagccagcgt catcgacgaa ctcaaagcca aaccgctgga
     6841 cagccttttg gaaatcggtg ccgtctttac cgttgtcgat ccgcgccagt tcatcaaccc
     6901 cgattacgcg caacaggcgt tgtataaaga ccaaatcggt atctgcgacg tattggccgc
     6961 cagcaaaacc gatttgtgcg cccccgaaca gcttgccgaa tttcacgaca aagcggcaaa
     7021 actgttcccg cccaaagcca aagtggtcga agtccgaaac gcgcaactcg acatccggtg
     7081 gctcgatatt cccgtcatcg aaaaatcacg ctaccgcctc aaagccctgc cggacaacac
     7141 catgggcttc cagtcgcaag gtttcacatt ccccgccaga cgcgatttcg acggcgaaaa
     7201 attgaccaac ttcttcaatg atttgcccaa gatgaccgaa ggactcgtcc gcgccaaagg
     7261 cgtgttccaa gtattgggaa cgtgggtgtg gctcaactgg gtagacgggc aatggggcgc
     7321 gaaccaagtg tcttggcggc gcgattcgcg ttttgaattg attgctaaat cgtttgatgc
     7381 ggatttaatc gaacaaaaac ttaaagatgc attggaatag cgttaacgcg ccttcagacg
     7441 gcatatgaaa tgaaaatgcc gtctgaaggt ctgaggagga tgggatgaaa ctcaaattta
     7501 aaaccgtttt aaccgcgctg cttttggcgg tttccctgcc gtctatggcg gcaacccatg
     7561 ttttgatgga aaccgatatg ggcaatatcc gtttggtttt ggacgagtcc aaagccccca
     7621 aaaccgttgc taatttcgtg cgctatgccc gaaaaggctt ttacgacgac accgtttttc
     7681 accgcgttat cgacggtttc gttatccagg gcggcggatt taccgaagac ttggtacaaa
     7741 aggcaaccga taaggccgtt gccaacgaat ccggtaacgg cttgaaaaac actgccggta
     7801 ccatcgccat ggcgcggacg gccgatccgg attccgccac cagccaattc tttatcaatc
     7861 tggcggacaa tgattcactc aactacaaaa acggacaata cggctatacc gttttcggca
     7921 gggtcgaaag cggcatgaac accgtttcca aaatcgcccg cgtcaaaacc gccacgcgcg
     7981 gcttttatca aaacgtaccc gtacagcccg tcaaaatccg tcgcgttgtt gtcgggcagt
     8041 aacacgcaga cagacgttca gacggcgtcg cccgtttccc aaaaaacgcc gtttaaatta
     8101 aaaaatattt taaaacagac agttgatatt gacaaattca aaccgaggat tttaaaatgc
//
LOCUS       DAWFJR010000085         8030 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWFJR010000085 DAWFJR010000000
VERSION     DAWFJR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.085"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(68..292)
                     /locus_tag="WGR04_001690"
     CDS             complement(68..292)
                     /locus_tag="WGR04_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ0795208.1"
                     /translation="MDAVQELERRIVELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(293..1681)
                     /locus_tag="WGR04_001691"
     CDS             complement(293..1681)
                     /locus_tag="WGR04_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ0795209.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGMMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(1767..2588)
                     /gene="prmC"
                     /locus_tag="WGR04_001692"
     CDS             complement(1767..2588)
                     /gene="prmC"
                     /locus_tag="WGR04_001692"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0795210.1"
                     /translation="MTFDKWLGLSKLPKNEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(2595..3038)
                     /locus_tag="WGR04_001693"
                     /pseudo
     CDS             complement(2595..3038)
                     /locus_tag="WGR04_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3103..4587)
                     /gene="tldD"
                     /locus_tag="WGR04_001694"
     CDS             complement(3103..4587)
                     /gene="tldD"
                     /locus_tag="WGR04_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ0795211.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVRERLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      4662..4765
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4832..6055
                     /gene="cytX"
                     /locus_tag="WGR04_001695"
     CDS             4832..6055
                     /gene="cytX"
                     /locus_tag="WGR04_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ0795212.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVLV
                     RLFFKKTQSLQRNPS"
     gene            6052..7152
                     /locus_tag="WGR04_001696"
     CDS             6052..7152
                     /locus_tag="WGR04_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ0795213.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVIKLGRQSIPLWRNIRGRLKTPAMMQENGSLIVWHGQDKPL
                     SNEFVRHLKRGGVADDEIVRWRADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEGLQAQYDWLIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSLARRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            7173..7790
                     /gene="thiE"
                     /locus_tag="WGR04_001697"
     CDS             7173..7790
                     /gene="thiE"
                     /locus_tag="WGR04_001697"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ0795214.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKALHGDE
                     LKREIARCVAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYIASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT         1732 a         2468 c         2168 g         1662 t
ORIGIN      
        1 cttaggtttg catcggcgga atattcaaac acagcctttt ttaaggaaat ccggatacgg
       61 cggcgcatca ataatgcggc ggaatctcgt cgcgcaggga atacggctct tgcgcgtcgg
      121 gattcctgtc ctgcattttt tgatacagca gcctcaactg agcctgctgc aaatccagcg
      181 tctgccgcaa ttccgccacc atcgcgttca ggccggcgat tacgtcctcc tgaagcgcgg
      241 attggatttc cagttcgaca atacggcgtt ccaactcttg aaccgcgtcc atttacagca
      301 ccatcgcggc aatccagccg gcaatcagca gcgggatgtt gtagtggatg aaggtcggga
      361 taacggaatc gcggatgtgg tcgtgctgcc cgtcggcgtt cagccccatc gtcggaccca
      421 gcgtggaatc ggacgcaggc gaaccggcat cgcccaacgc ccccgccgtg ccgacaatgg
      481 cgacggtggc aagcggcgaa aaacccaaac cgacacacaa aggcacataa atcgcggcaa
      541 taatcggcaa agtggaaaag gacgaaccga tgcccatcgt taccaaaagt cccaccacca
      601 gcatcgccaa cgccgccata cctttgctgt tgccgaatat cgccatactg ctttccacca
      661 gcggctggat atgcccggtc gcattcatca cggcggcaaa accctgcgcg gcaatcataa
      721 tgaagccgac catcgccatc atcttgatac cttcgccgaa tacgtcgttt gccttgtcgc
      781 ggttgatgac ccccaacatc ataaatacgg cgaaaccgag catcgcgccc aacaccagcg
      841 agtcttcata catcaactgg atggcaaagc atacggcaat ggcggcggcg gcggccaggc
      901 tgcggtaggc ggacggctgc ggacggtttg ccgcatcggc gttgcccgcc gtatcggcat
      961 tgttgctttg gtacaggcgc ggtttgcggt aatggacaaa cgccagcagg agtccggcca
     1021 gcattcccaa cgcgggaatc gccattgccg ccattacgtt gatgtttttc acatcaagct
     1081 gcggcgcggc ggaatggatg ttgcccaaca ggatttcatt caaaaaaatc gcgccgaagc
     1141 cgtaaggcag gaacatataa gtcgtaacca gcccgaaagt gatgacgcac gcaatcaggc
     1201 ggcggtcgat tttcaggcgg ttgaacacca aaagcagcgg cggaacaatc atcgggataa
     1261 aggcgatgtg gatggggatg atgttctgac tcatcatgcc catcacaagg atgatggaaa
     1321 gcagcagcca tttgaccgcg ccctcgcccg aacgcacgct gtcgggcata ccgccccggt
     1381 tcagcttgcg gacgaccgcg ccggcaagct gctgcggcag gccggaatgg gtaatcgcca
     1441 ttgcaaacgc gccgagcatc gcataagaaa gcgcaatctt cgcaccgcct tccaaacctt
     1501 tgttgaacac ggggataatc cccgcctggc tgacctgtcc cgccgcatcg gcaatgtttt
     1561 gcagcggcat acccgccacc gcgccgccga caaacgcgcc gaccgtcagg ctcaatacca
     1621 cgtgcacgcg cgacagcgac agcaccagca taacgattac ggcaactacg actgcattca
     1681 ttgtttatcg ctccaaacct ataaatgttt acatatcgaa acacatcata acccaataac
     1741 gggaaacccg ccaattttgc aaacaattat ttcaaatgct tcatatactt ccccagcgta
     1801 accctgtcca aacccgccaa gtccggcagg gtttccactc ccgaaaaacc attctccgcc
     1861 aacacgccgc gcaccgccgc gccctgatcg aaaccgtgtt ccagcaataa aaaaccgcct
     1921 tccgccaaac ggtcgggcgc gccttgcgcc aaggtgcgga tgcagcttag gccgtctgaa
     1981 aagtcggtca gcgcgatttg cggctcaaac cgcaaatcgc cttgcaacaa atgtttatcg
     2041 ccgttttcga tatagggcgg gttggacacg atgatgtccc atttcccttc agacggcata
     2101 tcggtgtcga accacgaacc gtgtgcaaat tcgacccgcg cgcccaaatc tgccgcattt
     2161 ttccgcgccg tttcaagggc gggcgtgctg atgtcggatg cgcgcacaaa cgcatcgggg
     2221 cgttcgagtg cgacggttac ggcaaccgcg ccgctgcccg tccccaaatc ccacacgcgc
     2281 ccgttttcgg gcaggcgcgc caatacggct tcgaccaaat gttcggtttc ggggcgcgga
     2341 atcagcacgc tcggattgac tgtaaagcgt ctgccataaa attcgcgcac acctaaaata
     2401 taggcaaccg gctcgccgtt cagacggcgt tgcgccagcc tgtccgcccg ctgtcggact
     2461 tcgtccggca tttcttcccc accccgcgtc aacaactgca cgcgcgtata ttccgaaaca
     2521 tattgcagca gcattcttgc ttcattttta ggcagttttg acaagcccaa ccatttatca
     2581 aacgtcattt ttatcccgtc tgccgctgat gcggcttttc tttccttatt ctttccggca
     2641 aacgtaccga tggtggcaac cgcaaatgcg gcataccaca aataaaatcc tgcaccgtag
     2701 cgcacaatat ccgatgtatt ccctgcttca tcgacgtata cggctttcac actgaaagcc
     2761 accaacgcca agccccaaaa tgccgcatgg acaggcacga ccttcttccg caacgccagc
     2821 aaaacaatgg ccgccaacca aacataattc gcatagaccg cacaatacct gatatccaaa
     2881 gaagcaaata tcgaccccaa aatcaaaacg gtcaaaccct ccatgcttcc atgattgccc
     2941 aaataaaatg caacattgga taaagacgct atccacaggg caaccgacac cagcaacatc
     3001 actatgggaa aacttgattt ccgattctgt tcctgcatgg ttttatccta atgtaaaagg
     3061 ccgcctgaaa acctttcaga cggcatcgtg ccggattccg cgtcagattg cgctgccgcc
     3121 gacggtcagt ccggcatcaa tccgcaaagt cggttgcccc acgccgacgg ggacgctctg
     3181 cccttctttg ccgcacacgc cgacaccgct gtcgagcgcg gtatcgttgc ctatcatcga
     3241 aacgtgtttc agcacttcgg gaccgttgcc gataatggtc gcgcctttga cgggatattg
     3301 cagcctgccg ccttccaccc accatgcttc ggacgcgctg aacacaaatt tgccgctggt
     3361 aatgtccacc tgtccgccgc cgaagttgac ggcgtaaatg cctttgtcga tggacgcgat
     3421 gatttcttcc ggctcatagc tgccgttttc cataaaggta ttggtcatgc gcggcatagg
     3481 ggcggaggcg tagctttcgc ggcggccgtt gccggtcgat tgcgtacccg tcaggcgggc
     3541 gttggtttcg tcctgcatat agccgactaa aatgccgtct tcaatcaata cggtgcggcg
     3601 ggtttcgttg ccttcgtcgt cgatgttgag cgaaccgcgc cggccggcaa tatcaccctg
     3661 atccacaacg gtaacgcctt tggcggcgac gcgctcgcct attctgccgg aaaagacgct
     3721 ggtccccttg cggttgaaat cgccttccaa accgtgtccg accgcttcgt gcagcaacac
     3781 gcccggccag ccgttgccca aaacgacggt catttcgcca gcgggcgcgg ggcgggattc
     3841 gaggttggtg agtgcctgtt tgacggcggc atcgacaaac tgccgcacca agttttcatc
     3901 gaaataagcc aagtcgtagc gtccgccgcc gcccgcgctg ccctgttcgc ggcgttcgcc
     3961 ctgtttggcg ataacggtaa cgttcaggcg caccatcggg cggatgtcgg cggcgtgttt
     4021 gccgtccaga cgggcgaggt aaaccatatc gtattcgcag gtcaaaccgg ccatcacttg
     4081 cacgatgcgc ggatcggcgg ctttggcgat tgcttccact ttgttcaaca gcgcgacttt
     4141 ggcggcggaa tcgaggccgg caatggggtc ggacgcggaa caaaccggct tgccgcgcgt
     4201 ttcagacggc attttggcgg acaccttgcc gcctgccgcc ccaatcgcgc ggacggcgcg
     4261 ggcggaacgg tttatcgaat cgatgcacag gctgtcggcg taggcaaagg cggttttatc
     4321 gcccgaaacg gcgcgcacgc ccacgccctg attgatttgg aagctgcccg atttgacgat
     4381 gccctcttcc aaatgccagc tttcataagc ggtgcgctgg cagtagatgt cggcataatc
     4441 gacgtggtgc gcgccgatga tgcacaggct tttggcgagc agttcggggg aaaggcggtt
     4501 ggcttcgagc agccgctctc gtacggcgga ataggtagga tgcatagtgt cggcgcataa
     4561 aaaatcaggg gcttgattat acggcatttg ttatagaaat gcgccgtgcc gccgaaaatg
     4621 taagaatttt gccaacgcac gttgctttgt gtaaacttaa agctttcttg tcggagtgcc
     4681 gccgccgggc ggctgagatt gcgaaagcag aatccgtaga acctgtcggg gtaatgcctg
     4741 cgtaggaaac aaaccgtcaa atgccttatc gggcttccgt tcccttttcc gcacttcccc
     4801 gcccaccttc cttcttttta aaggacgtca tatgtcgggc aatgcctcct ctccttcatc
     4861 ttccgccgcc atcgggctga tttggttcgg cgcggcggta tcgattgccg aaatcagcac
     4921 gggtacactg cttgcgcctt tgggctggca gcgcggtctg gcggctctgc ttttgggtca
     4981 tgccgtcggc ggcgcgctgt tttttgcggc ggcgtatatc ggcgcactga ccggacgcag
     5041 ctcgatggaa agcgtgcgcc tgtcgttcgg caaacgcggt tcagtgctgt tttccgtggc
     5101 gaatatgctg caactggccg gctggacggc ggtgatgatt tacgccggcg caacggtcag
     5161 ctccgctttg ggcaaagtgt tgtgggacgg cgaatctttt gtctggtggg cattggcaaa
     5221 cggcgcgctg attgtgctgt ggctggtttt cggcgcacgc aaaacaggcg ggctgaaaac
     5281 cgtttcgatg ctgctgatgc tgttggcggt tctgtggctg agtgccgaag tcttttccac
     5341 ggcaggcagc accgccgcac aggtttcaga cggcatgagt ttcggaacgg cagtcgaatt
     5401 gtccgccgtc atgccgcttt cctggctgcc gcttgccgcc gactacacgc gccacgcgcg
     5461 ccgcccgttt gcggcaaccc tgacggcaac gctcgcctac acgctgaccg gctgctggat
     5521 gtatgccttg ggtttggcag cggcgttgtt caccggagaa accgacgtgg caaaaatcct
     5581 gctgggcgca ggtttgggtg cggcaggcat tttggcggtc gtcctctcca ccgttaccac
     5641 aacgtttctc gatgcgtact ccgccggcgc gagtgcgaac aatatttccg cgcgttttgc
     5701 ggaaacaccc gtcgctgtcg gcgttaccct gatcggcacg gtacttgccg tcatgctgcc
     5761 cgttaccgaa tatgaaaact tcctgctgct tatcggctcg gtatttgcgc cgatggcggc
     5821 ggttttgatt gccgactttt tcgtcttgaa acggcgtgag gagattgaag gctttgactt
     5881 tgctggactg gttctgtggc ttgcgggctt catcctctac cgcttcctgc tctcgtccgg
     5941 ctgggaaagc agcatcggtc tgaccgcccc cgtaatgtct gccgttgcca ttgccaccgt
     6001 actggtgcgc cttttcttta aaaaaaccca atctttacaa aggaacccgt catgacccgt
     6061 atcgccatcc tcggcggcgg cctctcggga aggctgaccg cactgcagct tgcagaacaa
     6121 ggttatcaga ttgcactttt cgataaaggc tgccgccggg gcgaacacgc cgccgcctat
     6181 gttgccgccg ccatgctcgc gcctgcggcg gaagcggtcg aagccacgcc tgaagtcatc
     6241 aaactgggca ggcagagcat cccgctttgg cgcaacatca gaggccgtct gaaaacgcct
     6301 gccatgatgc aggaaaacgg cagcctgatt gtgtggcacg ggcaggacaa acctttatcc
     6361 aacgagttcg tccgccatct caaacgcggc ggcgtagcgg atgacgaaat cgtccgttgg
     6421 cgcgccgacg acatcgccga acgcgaaccg caactcggcg gacgtttttc agacggcatc
     6481 tacctgccga ccgaaggcca gctcgacggg cggcaaatat tgtctgcact tgccgacgct
     6541 ttggacgaac tgaacgtccc ctgccattgg gaacacgaat gtgcccccga aggcctgcaa
     6601 gcccaatacg actggctgat cgactgccgc ggctacggcg caaaaaccgc gtggaaccaa
     6661 tcccccgagc acaccagcac cctgcgcggc atacgcggcg aagtggcgcg ggtttacaca
     6721 cccgaaatca cgctcaaccg ccccgtgcgt ctgctccatc cgcgttatcc gctctacatc
     6781 gccccgaaag aaaaccacgt cttcgtcatc ggcgcgaccc aaatcgaaag cgaaagccaa
     6841 gcccctgcca gcgtgcgttc agggttggaa cttttatccg cgctttatgc cgtccatcct
     6901 gccttcggcg aagccgacat cctcgaaatc gctaccggcc tgcgccccac gctcaaccac
     6961 cacaaccccg aaatccgtta cagcctcgcc cgacgcctga ttgaaatcaa cggccttttc
     7021 cgccacggct tcatgatttc ccccgccgta accgccgccg ccgtcagatt ggcagtggca
     7081 ctgtttgacg gaaaagacgc gcccgaacgt gatgaagaaa gcggtttggc gtatatccga
     7141 agacaagatt aaagccgtct gaaaggaacc ccatgacctt cccgccactc aaatccccgc
     7201 tcaaattcta cgccgtcgtc cccaccgccg attgggtggg gcgcatggtc aaagcaggtg
     7261 ccgacacggt gcaactgcgc tgcaaggccc tgcacggcga tgaattgaaa cgcgaaatcg
     7321 cccgctgtgt cgccgcctgt cagggcagcc gtacgcagct tttcatcaat gaccactggc
     7381 gcgaggcaat cgaagcaggc gcgtacggcg tgcatctcgg acaagaagac atggacaccg
     7441 ccgaccttgc cgccatcgcc gccgccggtt tgcgcttggg tttgagtacg cactccgttg
     7501 ccgaactcga ccgcgccctg tccgtacacc ctagctacat cgccagcggc gcgattttcc
     7561 cgaccacgac caagcaaatg cccaccgccc cgcaaggctt ggacaaactg tgcgagtatg
     7621 tgaaacaggc aggcggcacg cccgtcgtcg ccatcggcgg tatcgacttg aacaacgccc
     7681 gagccgtact cgccaccggc gtttcctcac tcgccgccgt ccgcgccgta accgaagcgg
     7741 caaatcccga agcggtggtt aaagcgtttc aggctttgtg ggatggataa aaccgaaaga
     7801 agaaaattca attgccgtgt aggcaaaact tagcccgtta tcgcaaacat acttaacttt
     7861 aaatgtggca tatcatcaaa ttccgtcatt cccgcgcaag cgggaatccg ccttaaaact
     7921 tgagaaacca tcatttgaaa aacagtttcc gaatttcaaa aatggattcc cgcctgcgcg
     7981 ggaatgacgg caaccggccg gttgcgtatc aaaaaataaa gtaattcggc
//
LOCUS       DAWFJR010000086         8015 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWFJR010000086 DAWFJR010000000
VERSION     DAWFJR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8015
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.086"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(100..459)
                     /gene="rnpB"
                     /locus_tag="WGR04_001698"
     ncRNA           complement(100..459)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WGR04_001698"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(538..1641)
                     /gene="prfB"
                     /locus_tag="WGR04_001699"
     CDS             complement(538..1641)
                     /gene="prfB"
                     /locus_tag="WGR04_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ0795215.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVVGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1744..2637)
                     /locus_tag="WGR04_001700"
     CDS             complement(1744..2637)
                     /locus_tag="WGR04_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ0795216.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKLVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIQL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2979..3428
                     /locus_tag="WGR04_001701"
     CDS             2979..3428
                     /locus_tag="WGR04_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0795217.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3502..3810
                     /locus_tag="WGR04_001702"
     CDS             3502..3810
                     /locus_tag="WGR04_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795218.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3926..4768
                     /locus_tag="WGR04_001703"
     CDS             3926..4768
                     /locus_tag="WGR04_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ0795219.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEC
                     RRAA"
     gene            4758..5504
                     /locus_tag="WGR04_001704"
     CDS             4758..5504
                     /locus_tag="WGR04_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ0795220.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETLQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLTQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5526..6113
                     /locus_tag="WGR04_001705"
     CDS             5526..6113
                     /locus_tag="WGR04_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ0795221.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6119..6298
                     /locus_tag="WGR04_001706"
     CDS             6119..6298
                     /locus_tag="WGR04_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ0795222.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6530..6970
                     /locus_tag="WGR04_001707"
     CDS             6530..6970
                     /locus_tag="WGR04_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795223.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQSYTASSLRPMSILSGTLTEKQYETVCGKKL"
     gene            complement(7499..7856)
                     /locus_tag="WGR04_001708"
                     /pseudo
     CDS             complement(7499..7856)
                     /locus_tag="WGR04_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467771.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7871..8015
                     /locus_tag="WGR04_001709"
                     /pseudo
     CDS             7871..8015
                     /locus_tag="WGR04_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         2128 a         2094 c         1770 g         2023 t
ORIGIN      
        1 ccgtaccggt ttttgttaat ccactataaa aacacaacct aaataaaaat gccgtctgaa
       61 ccatatttca ggtttcagac ggcatttgcg tgtcggatgc acaccggacg ggcggtaagc
      121 cgggttctgt ctcggacagt cattcctcta ggcataccgt taccggtatg ctcaagcaac
      181 ctacccgaac gctcggcggg cagcgtcatt gcgttctgtt tggtcttgct ccgaatgggg
      241 tttggcctgc cgcatattgt taccaaatgc gcggtgcgcc cttaccgcac cttttcaccc
      301 ttgcctgtgc tgccaaagca gccatcggcg gttttgcttt ctgttccact ttccgtcgcg
      361 ttaccgcgcc cggccgttaa ccggcattct accctgcgga gcccggactt tcctccccgt
      421 atgccttacg cgatacgcgg cgactgtctg cccgtcccgt gtgcggcgcg gattataaca
      481 cgaaacgcaa aaatgccgtc tgaaacggta caggtttcag acggcataca gcctaaacta
      541 cacgccctgt ttcaggctgg cttcgataaa gccgtccaaa tcgccatcca atacggcttt
      601 ggtgttgccg acttcgtagc ctgtacgcaa gtctttaatg cgtgaggaat ccaaaacata
      661 cgaacggatt tggctacccc aacctacatc ggatttacct tcttccaacg cctgtttctc
      721 ttcgttgcgt ttgcgcattt ccaattcata cagtttggat ttcaacattt ccatcgcggc
      781 cgctttgttg gcgtgttgcg aacggtcgtt ttgacattgc accacaatcc ccgtcggctc
      841 gtgggtaata cgcacggcgg agtcggtttt attgatgtgc tgaccgcccg cgcccgatgc
      901 gcgataggtg tcgatacgca aatcggcggg gttgatttcg atttcaatgg aatcatcgat
      961 ttcagggtaa acgaacacgg aggcaaacga agtatggcgt ttgttgttcg agtcaaacgg
     1021 tgaatagcgc accaagcggt gaacgccggt ttcggtacgc agcaaaccat aggcatattc
     1081 gccttccact cggatagtcg cgcggttgat gcccgcgatt tcgccgtcgt cttcttcgag
     1141 gatttcgatt ttgaagcctt tgcgctcggc gtagcggctg tacatacgga acagcatacc
     1201 cgcccagtct tccgcttccg taccgcctgc gcctgcggta atgtcgataa agcagttgtt
     1261 cggatcggcg ggctggttga acatccgttt gaactccaaa tccgccatct gtttttccag
     1321 ccccgccaca tcttcctgca cggcggcaaa accttcttcg tcgttttctt cgacggtcat
     1381 ttcaatcagc atgcggttgt cttcgatgcc cgaagcgatg ttgtcgagcg tcaacacgat
     1441 gccttcgagg attttgcgct ctttgccgat ttcttgggcg cgtttcgggt cgttccaaag
     1501 ttcggggtct tcggaaagac cgacaacttc ttccaatcgg tctttcttac cctgataatc
     1561 catataaact cggatgtctt cgctgcgctt ttccaaatca ttcagggtat tgttgagctg
     1621 gttgattact tcggcttcca tgattctttt gttctttcaa aattttaggg gcgtattgta
     1681 cgggattcgg gtattttttt ctatggataa agccttctgg aaacacgttc ggacggcata
     1741 gcgtcaataa cggtatgccg ccagtttgcg tttgatttca ggcaatgcgg cacgtgctgc
     1801 ctcctcaccc aactggatgg cgcgtttttt ctgatcgaat ccgccgactg cacccaaatc
     1861 caaaacctgt ggtttgataa ctacatccgc ctgccccaac tcattttgca acgcagaaac
     1921 gctcattacg ttcagcgtct gatcgagata agagaagaaa ccttggctga tgtttttgcc
     1981 cggacgggcg gaaatatcga cggcaatcac gaaattcgcc ccctgccgcc gggcggcact
     2041 gacgggcacg ggctgcgaca gaccgccgtc aacatatgta tgcctgccga tgataacggg
     2101 ttggaacaca ttgggaatgg cggcggaagc gcgcacagcc tgcccggcat tcccctgatt
     2161 gaaagcgacg gccttgccgg tttcaaaatc agtagcaacg gcggcaaatt tgatgggaaa
     2221 ctgctgaatc tgcctgccgc cgacttttcg gttgatgtaa ttttgcagct tttcgccttt
     2281 gataaaacca ctggtggaca aggttaaatc gaccaaatcg gttttgccta aaatttcggc
     2341 ttccaattcg aggcggtcgg gcgacatacc cgatgcaaaa aggctgccga caatcgaacc
     2401 tgccgatgta ccggtaacca ccttcacagg aataccgttt tctttcaaaa ccttaataat
     2461 acctacatgg gcaaatcctt tagatgcgcc gccaccgagt gccaaaccga ccactgcggc
     2521 gggtttggcg gtttgcacca gcttgcgggc agcattattt cccgccgtgc cgcaggcggc
     2581 aagcaacgcg gcggcggcga ttgccaaaag cggtctgatt tttgaaaacg ttaccatatt
     2641 ttccattcct ttatatatca caccccgtca aaaagaggga ttgcttttct taacaacccc
     2701 ctttgacagc caagcaaatg gggactttgt taagtcatca tcaaaattaa tatttctttt
     2761 tttcctttac ggaaattata tttgaaggca tactatccaa ggtgggaatt atctcacaac
     2821 accgccgtta tccaaatatc ccgccttttt ccctttcttt ccatcaaaat actttctttt
     2881 tatattcatt aacttgttaa atcattggct gccgggtgtc agtttttccg acaaaatccg
     2941 tctaatgggg tatcaacaga accaaaacag gaacacttat gaaaatcgga acaacttggc
     3001 agacggcatc cgctatgctg gttttgcgtc tgtttgccgc atatgaattt ttggaatcgg
     3061 gtttgcaaaa atggaacggg gagaattggt tttccgaaat caacgatcag tttccattcc
     3121 cgttcaactt gctgccggac gcgttaaact ggaatctcgc catgtatgcg gagcttttac
     3181 tgcccgtact gttgcttttg ggtttggcaa cgcgtctgtc ggcattaggg ctgatggtcg
     3241 ttaccgccgt cgcttgggct gcggttcacg ccggttcggg ttacaatgtc tgcgacaacg
     3301 gttataaaat ggctttaatt tatatcgtgg tattaatccc gctgcttttc cagggtgcgg
     3361 gcggatggtc gctggatacg ctgctgaaaa aacggttttg cccccaatgc cgtctgaagc
     3421 aagattgatt cagtcgtgga atctgacttt aaacattcca accttatctc gttaacttga
     3481 tattctgaaa aggaaataga aatgaacaaa aacattgctg ccgcactcgc cggtgcttta
     3541 tccctgtctt tggccgccgg tgcagttgct gccaacaaac cggcaagcaa cgcaacaggc
     3601 gttcataaat ccgcccatgg ctcttgcggc gcgtccaaat ctgccgaagg ttcgtgcggc
     3661 gcggctggtt ctaaagcagg cgaaggcaaa tgcggcgagg gcaaatgcgg tgcgaccgta
     3721 aaaaaaaccc acaaacacac caaagcatct aaagccaagg ccaaatctgc cgaaggcaaa
     3781 tgcggcgaag gcaaatgcgg ttctaaataa tcccacccct tcaaaccaag ccgcgttttt
     3841 cagtaaaatg cggctttttt aacggcaaac aaagattttt taacaagcac atcattcttt
     3901 tgtgccatcc gaaccgggta aaaatatgat tcaacacgca ggcttgggct accgccgcga
     3961 cttggcggaa gactttctct cgctttccga aaacagcccg atatgcttta tcgaagccgc
     4021 accggaaaac tggctgaaaa tgggcggctg ggcgcgcaaa cagtttgacc gtgtggcgga
     4081 acggctgccg ctggcgttgc acggattgtc tatgtcgctg ggcgggcaag caccgctgga
     4141 tactgatttg atagacggca tcaaagaaat gatgcgccgt tacgattgca cgtttttctc
     4201 cgaccatttg agctactgcc acgacggcgg tcatctttac gatttgttgc cgctgccctt
     4261 taccgaggaa atggtgcatc atacggcgcg gcgtatccgc gaagtgcaag accgtttggg
     4321 ctgccgcatc gccgtggaaa acacgtccta ctatctgcat tccccgcttg ccgagatgaa
     4381 cgaggttgag ttcctcaacg cggttgcacg tgaggccgat tgcggcattc atctggacgt
     4441 gaacaatatc tacgtcaacg ccgtcaatca cggcctgttg tcgccggagg cttttttgaa
     4501 aaacgttgac gcagatcgcg tgtgctatat ccatattgcc ggacatgacg tggaaacgcc
     4561 ggaattgttg attgatacac atggcgcggc agttttgccg actgtttggg acttgctcga
     4621 acttgcctat gccaagctgc cgacgattcc gcccaccctg ttggaacgcg attttaattt
     4681 cccgcctttt gccgaactcg aagccgaagt cgccaaaatc gccgattatc aaacgcgtgc
     4741 cggaaaggaa tgccgccgtg cagcctgaaa cctccgccca ataccagcac cgtttcgccc
     4801 aagccatacg cgggggcgaa gccgcagacg gtctgccgca agaccgactg aacgtctata
     4861 tccgcctgat acgcaacaat atccacagct ttatcgaccg ctgctatacc gaaacgctgc
     4921 aatactttga cagcgaagaa tggagccgtc tgaaagaagg tttcgtccgc gacgcgcacg
     4981 cccaaacgcc ctattttcaa gaaatccccg gcgagttcct acaatattgc caaagcctgc
     5041 cgctttcaga cggcattttg gcactgatgg attttgaata tacccaattg ctggcagaag
     5101 ttgctcaaat tccggatatt cccgacattc attattcaaa tgacagcaaa tacacaccct
     5161 cccctgcggc ctttatccgg caatatcgat atgatgttac cgatgatttg catgaagcgg
     5221 aaacagcctt gttaatatgg cgaaacgccg aagatgatgt gatgtaccaa acattggacg
     5281 gcttcgatat gatgctgctg gagataatgg gttcctccgc gctttcgttt gacaccctca
     5341 cccaaaccct tgtcgaattt atgcctgagg acgataattg gaaaaatatt ttgcttggga
     5401 aatggtcagg ctggactgaa caaaggatta tcatcccctc cttgtccgcc atatccgaaa
     5461 atatggaagg caattccccg ggccaaaacc atctatccgc ataaaattac cttgttcccg
     5521 atactatgcc gctacccgac ctgaccgatg ccgaattaat agagtcgcgt aaactgcttc
     5581 tgcattttgc gcggcttcag ttgcccgacc accctgattt ggctgaagat ttagtgcagg
     5641 aaacattgct gtccgcatac agcgcaggcg acagttttca aggcagggca cttgtcaaca
     5701 gctggctttt tgccatattg aaaaacaaaa ttattgacgc attacgtcaa atcggaaggc
     5761 agaggaaagt ctttaccaca ctggatgacg agctactgga tgaagcattt gaaagccatt
     5821 tttcccaaaa cgggcattgg acgccggaag ggcagccgca acattggaac actccggaaa
     5881 aatcattaaa caacaacgaa ttccaaaaaa ttctgcaaag ctgcctatac aacttgcctg
     5941 aaaacaccgc acgggtattt accctgaagg aaatactcgg tttttcatcc gacgaaatac
     6001 aacaaatgtg cggtatcagc acgtccaact accacaccat tatgcaccgc gcccgagaat
     6061 cattgcgcca atgcctgcaa atcaaatggt tcaaccaaga aaacccgaag taaacgttat
     6121 gaaaaaatgc cgcgatatcg ccctgcttct ttccaaacat caggaccggg aaaccacccc
     6181 gggcgagaag atttccatat acacacacct gctgttctgt ccgtattgcc gtgaatataa
     6241 aagacaactt caaaccatca aaagatcact ggcaaaaaca accagaactt caaaataaat
     6301 gccgtctgaa aaggcttcag acggcataag ctgacggaaa caaatcaaac cgatttactg
     6361 ttatcggcag ttcatccata atacacgctt caaaagcagc atattcctcc atacggaata
     6421 tataaatacg caaaacacga aggctgcatc aatttgcttt atttgcctta tttcacagac
     6481 ggcgctaccc ctcccgccca acccgttctt tctgaatgag cagatttcaa tgattaagga
     6541 aaccctaatg cgcccaatct tcctatcttt cgttttactc cctattttga taaccgcctg
     6601 cagcacaccg gacaagtctg cccgatggga aaatatcggc acaatctcaa acggcaatat
     6661 tcatacatat atcaataaag acagcgtgag aaaaaacgga aatctgatga ttttccaaga
     6721 taaaaaagtt gttaccaatc taaaacaaga acgttttgcc aacacccccg catacaagac
     6781 tgccattgcc gagtgggaaa tccactgcaa caacaaaaca taccgcttaa gttcgctaca
     6841 gttgtttgat acaaaaaaca cggaaatttc cacacaaagc tacacagcct cttccctccg
     6901 cccgatgagc atcctgtccg ggacattaac cgaaaaacaa tatgaaaccg tatgcggaaa
     6961 aaaactctga ttgcaactta tatacaaact tacccacaaa ccttatcata aaaatgccgt
     7021 ctgaaatact gaaatatcag catttcagac ggcattttgc cattccctga aaattatcca
     7081 caaagttatc cacattattt tttaaaaccg gcttccatcc gaaatatagt ggattaacaa
     7141 aaatcaggac aaggcgacga agccgcagac agtacagata gtacggcaag gcgaggcaac
     7201 gccgtactgg tttaaagtta atccactata taaactcgct atacaatttc actatccaaa
     7261 cgtaaattgt tccattgata cacaaaactt cttaccccca taattttgat aaagcatttc
     7321 ttacattccc ggctccgtcc cgtaaccaac acagcctcta aaaaataatc gcttgttttg
     7381 acaccgatac actcatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
     7441 cagtacagat agtacggcaa ggcgaggcaa cgctgtactg gtttaaattt aatccactat
     7501 acaaatacag aaactcaaga aaataacctt gtgtattgac catctcaagc aattcagaaa
     7561 aatcaagaaa ttttctgacc gtaaacaaac gtttccctaa aaaaaacgat gtcttcaaaa
     7621 atatcgacca aataatcatc ttcgccgttt tcatttttat gggttaaaac aacacgggaa
     7681 aaatctgttt tcagatgctt gcccgcttga ttgttcggat tattgtccgg aacgacaaaa
     7741 ccgtcctcaa aatcaaagta gacgttgcgt ccttctacct ttatctctgt gcaataacaa
     7801 tcatgcagag aaataccatc cgaaaaattt aggggctgta ctagattagc agatatgtta
     7861 ccctcgaaat atgaagataa cgcactgcaa atgaaagaaa aaagtacaga aagaactgct
     7921 ccgttttttt gtactcgaag ttaccgcccg ttctgccgcc gatattttgg gtatccatcc
     7981 caattcggca gcactgttct accgtaaaat ccgca
//
LOCUS       DAWFJR010000087         7978 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWFJR010000087 DAWFJR010000000
VERSION     DAWFJR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.087"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            22..861
                     /locus_tag="WGR04_001710"
     CDS             22..861
                     /locus_tag="WGR04_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0795224.1"
                     /translation="MQPQKTFSSLLFSSLLFSSLLFSSLLFSSLLFSSLLFSSAAQAA
                     SENSSRSPYYVQADLAYAAERITHDYPQATGANNTSTVSDYFRNIRAHSIHPRVSVGY
                     DFGGWRIAADYASYRKWNNNKYSVNTKVLKETQDNWQELKTENQENGTFHAASSLGLS
                     AIYDFKLNDKFKPYIGARVAYGHVKHQVHSVETKTTTVTSKPNGKNLKGGPVSSREPS
                     KPPYHESHSTSSLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMR
                     YHF"
     gene            875..1045
                     /locus_tag="WGR04_001711"
     CDS             875..1045
                     /locus_tag="WGR04_001711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795225.1"
                     /translation="MPSEPSDGIFDAPAVYRRGAGVGKCPNRHSRQHRNLEIRHSRAG
                     GNPDLSAQKLIG"
     gene            1975..2421
                     /gene="yciA"
                     /locus_tag="WGR04_001712"
     CDS             1975..2421
                     /gene="yciA"
                     /locus_tag="WGR04_001712"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ0795226.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPILAGLLPTP"
     gene            complement(2544..3365)
                     /locus_tag="WGR04_001713"
     CDS             complement(2544..3365)
                     /locus_tag="WGR04_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0795227.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFGDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDEKAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFTGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            3484..3942
                     /locus_tag="WGR04_001714"
     CDS             3484..3942
                     /locus_tag="WGR04_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ0795228.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PEFTRMGKMVKDEEPYDVEKFKQAAAAFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(4086..5222)
                     /gene="lst"
                     /locus_tag="WGR04_001715"
     CDS             complement(4086..5222)
                     /gene="lst"
                     /locus_tag="WGR04_001715"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ0795229.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNHGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFKQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYPPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKNQSHSKSK"
     gene            5323..5427
                     /locus_tag="WGR04_001716"
                     /pseudo
     CDS             5323..5427
                     /locus_tag="WGR04_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(5425..5586)
                     /locus_tag="WGR04_001717"
     CDS             complement(5425..5586)
                     /locus_tag="WGR04_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795230.1"
                     /translation="MAYFGGFVRALFDLGVFERGSVKGRGKWFSRVWWRSEVLCLMCG
                     GIFSVKLIL"
     gene            5655..7880
                     /locus_tag="WGR04_001718"
     CDS             5655..7880
                     /locus_tag="WGR04_001718"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ0795231.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLLANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKDGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTDGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT         2146 a         2338 c         1765 g         1729 t
ORIGIN      
        1 cgaatacgga gtcgaaaatg aatccagccc caaaaaacct tctcttctct tctcttctct
       61 tctcttctct tctcttctct tctcttctct tctcttctct tctcttctct tctcttctct
      121 tctcttctct tctcttccgc agcgcaggcg gcaagtgaaa acagcagccg cagcccgtat
      181 tatgtgcagg cggatttagc ctacgccgcc gaacgcatta cccacgatta tccgcaagca
      241 accggtgcaa acaacacaag cacagtaagc gattatttca gaaacatccg tgcgcattcc
      301 atccaccccc gggtgtcggt cggctatgat ttcggcggct ggaggatagc ggcagattat
      361 gccagttaca gaaaatggaa caacaataaa tattccgtca acacaaaagt gttgaaagaa
      421 acccaggaca actggcaaga actgaagacg gaaaatcagg aaaacggtac attccacgcc
      481 gcttcttctc tcggcttatc cgccatttac gatttcaaac tcaacgataa attcaaaccc
      541 tatatcggtg cgcgcgtcgc ctacggacac gttaaacatc aggttcattc ggtggaaaca
      601 aaaaccacga ctgttacctc taaaccaaac ggaaaaaatc taaagggagg acctgtctca
      661 tcaagagagc ccagcaaacc tccctatcac gaaagccaca gcaccagcag cttgggtctt
      721 ggtgtcatcg ccggtgtcgg tttcgacatc acgcccaagc tgaccttgga caccggatac
      781 cgttaccaca actggggacg cttggaaaac acccgcttca aaacccacga agtctcattg
      841 ggcatgcgct accacttctg attccccgat accgatgccg tctgaacctt cagacggcat
      901 ttttgatgca cctgccgttt acaggcgcgg ggcgggcgta gggaaatgcc cgaaccgtca
      961 ttcccgacaa caccgcaatc tcgaaattcg tcattcccgc gcaggcggga atccagacct
     1021 gtccgcacag aaacttatcg gataaaacgg tttctttaga ttttacgtcc tagattccca
     1081 cttccgtggg aatgacggtt cagttgcatt cccgacaaca acgcaatctc gaaacccgtc
     1141 attcctgcgc aggcgggaat ccagacccct gacgcggcgg gaatctatcg gaaatgactg
     1201 aaaccccgag attctagatt cccactttcg tgggaatgac gtggtgcagg tttccgtatg
     1261 gatggattcg tcattcccga caacaccgca atctcgaaac ccgtcattcc cgcgcaggcg
     1321 ggaatccagc cccccgacgc ggcgggaatc tatcggaaat gactgaaacc ccgagatgct
     1381 agattcccac tttcgtggga atgacggttc agttgcgttc cgacaacacc gtaatctcaa
     1441 aacccgtccg acaacaccgc aatcttaaaa cccgtcattc ccgcacaggc gggaatccag
     1501 acctgtccgc acagaaactt atcggataaa aacagttgct caaaccccgc gttctagatt
     1561 cccactttcg tgggaatgac ggttcagttg cgtaggactg ggttgtcgaa agggacggat
     1621 tcgatggatt cgatgaaaac ggtagaaatg ctggattgat ggaaatggcg ggctgaagcc
     1681 cactccacag ccccgcccta caatacgcct tgcgaatctg tcccgcaccc gcccttgtat
     1741 gcctcaaatg ggttaaacta cgcccttccc gacttcccgg gtacaggtcc gatttatagt
     1801 ggattaacaa aaatcaggac aaggcgacga agcctcagac agtacaaata gtacggaacc
     1861 gattcactcg gtgcttcagc accttagaga atcgttctct ttgagctaag gcgaggtaac
     1921 gccgtactgg tttttgttaa tccactatat atttcaataa aacaaggaaa ctttatgcag
     1981 cacgaagaag gcaaccgcca acgccctcaa ggcgaactgc tcctgcgtac cgtcgctatg
     2041 ccgcgcgata ccaatcccaa ccaagacatt ttcggcggct ggattatgtc gcaaatggat
     2101 ttgggcggcg gcatattggc ggcggaaatc gcgcggggac gcatcgttac cgtcgccgtt
     2161 caggaaatga acttcatccg cccggtcaag gtcggcaacg tcgtctgctg ctacgggcat
     2221 tgcgtccgcg tgggcaatac ttccctccag cttaaagtcg aagtctgggt gaaaactttg
     2281 atgaacgatt gcgttaccga agaccgccac ctcgtaaccg aagccgtgtt cacttatgtc
     2341 gccatcgatg cggaaggcaa tccgcgcccg attccgaaag aaggcaaccc catattggcg
     2401 ggcttattgc ctactccgta aaaatacccg taaaaatacc cgtaaaaata cccgtaaaaa
     2461 tacccgtaaa aatacccgta aaaatacccg taaaaatacc cgtaaaaatg ccgtctgaaa
     2521 ctgtcttcag acggcattcc gtatcatttg aaccaggacc tgatgcggga aaacagcgac
     2581 atactctgtt tttcaaagtc ttcccgcact tccggcacgg ctttttttat cactttcatt
     2641 cctgtaaaag tctgcgccac gggcataatt tcaatcgtgt tcacattcat atgcgccggc
     2701 cggcggtaca gccacaatgc ggtttccgca atatcttcgg ggcggataaa ttccacaccc
     2761 tcatacacgc ctgctgcctt ctcgtcatcg cctttgaagc gcacgttgga aaactccgta
     2821 ttgccgcaca aacccggctc gatattggta acgcggatgt tcttatccgc caactccgcg
     2881 cgcaaattca ggctgaactg gcgcacaaac gccttggtcg ccccgtaaac gttgctgccg
     2941 gcataagcat aattacccgc aatcgaaccc aaattcatca catagccgcc gccacgttcc
     3001 accatttgcg gcaaaatctt gcgcgtcagg aacgtcaaac ccaaaacatt ggtttgaatc
     3061 attgtttccc aatctccaaa atccgccttg tccgccgtgt ccaaacccaa agccagcccg
     3121 gcattgttga tgaggcagtc gatgtcggaa aattcatcgg ggatgccgtt taaggcgttt
     3181 tccaccgact cgcggcgcga cacgtccatt tccaaagggt aaaacaaagc acccaattca
     3241 tccgccaagg cctgaagcct gtccgcacgg cgcgccgcac cgataacgcg gtatcccgcc
     3301 ccgacaaacg cacggcacat cgcttcgccg aatcctgccg aagcaccggt aattaaaaca
     3361 gccattgttt ttcctttctt tctcgtttcc caaacctgtt tcaggtatca taacgctctt
     3421 cagacggcat cgcgccggct gaaaatgaca acaaccatac caccacgaca ggacacattc
     3481 aatatgaaaa cccaaatcag ccttgctgct gccgccatca cactgctcct ttccgcctgc
     3541 ggaggcagcg gaataccttc ccaacccaaa ggcgaaattt ccgaaaaccg caccgccgcg
     3601 ttcaaatcca tgatgcccga attcacacgc atgggcaaaa tggtcaaaga cgaagaacct
     3661 tacgatgtcg aaaaattcaa acaggctgcc gcggcgtttg ccgaaagcag caagaaaccg
     3721 ttcacacttt ttgagtccga tccgcaaggc aacggccgcg ccctgcccgc cgtttggtcg
     3781 gatggtgcaa aatttgaagc cgaaaaaaca aaattcgccg ccgccgtcga aaaactcaac
     3841 gccgcagcac aaaccggcaa actggatgaa atcaaagccg cctacggcga aaccggcgca
     3901 agctgcaaat cctgccacga cagtttccgc gcgcccgaat aacatccaaa acaaaacaat
     3961 gccgtctgaa gccttcagac ggcattttct ttgccatttc agcacacgca aagcaatcag
     4021 aaattatcgt caattcaaat gaaaaaacgc tatccttatt tttaccccac gcactccgcc
     4081 attctctatt ttgatttgct atgcgattga tttttatcgt caaatgtcaa aatcgggatg
     4141 ccggattggg taaacagggc atacaccggc ttgagccaat aatcttccgg aagggaagcc
     4201 ggtttcaatg cgtaaacgtg cacattggga aaatccttca tcgtcaacgc cgcgccgctg
     4261 aaaaaggtat aaatttcata cctcgtatgc gggtttttct taatctcgcg caaaatatag
     4321 tcttcgatga catagggcga atttaatgtg gttacgccgg aaagcccgta ggtttggcgg
     4381 gggtgcggtg cgacatattg tatgttgaag ttttttgccg ccttttccga aatttccttc
     4441 atctccttgt cgggcgaacc caaaagtatc cgcaccgtgc cgcccgtttc gtcccccgcc
     4501 ttcagttcgg acgcatcgaa cagcggcggg taagtcatct tgcggcggcc gtcgtccata
     4561 atgtttttca aacccttgaa tatcgtgtaa tgctcgtcgg aagcattacg ggttttggcg
     4621 atgctccaat ctccgatcat catccgggca aaattccgct tgatcgtccc gtttacagaa
     4681 aactcatcgc ccaaatagct gctgctttga attaaattgc ctgtcccgtc gtcaaaggtt
     4741 ttgatttccg catccgggta agtgctcaaa aaggcggcaa tgctgacttt ttccaaactt
     4801 gccaaataaa tccgcttgac tttcggcagc agcatcgact ttaccttcag ctccgccatc
     4861 gtcggaatga aattaaacga tttgttcaaa ccgtagggca ggtggaaaaa atacgcccgc
     4921 tccgccttat cctttatctg cttgaaataa taatcgtatt tttcattcct gttttcagac
     4981 atcagcacca cataaaaccg ctcccccgga tgctgcgcca taatcctttc cgccaccttc
     5041 atctgcaata tggtatagca gaaaatcaga ttgaccggtt ccccctcttc attgaagagt
     5101 ttgtccttca gcagggaaac cgcattcctt tccccatgat ttacccggtc aaatgtataa
     5161 aatatcccga aacaaaaaac aatcaaacac aacacggtca aacaagcctt tttcaagccc
     5221 atatccctaa aactccattc cgacaaattg aacatacgcc ccgagtgtcc cgggacggaa
     5281 aaatgcggaa ttttagcaga tattccgaca gttaccgaat attatagtgg tttaaattta
     5341 aatcaggaca aggcgacgaa gccgcagaca gtacaagcag tacgacaagg caaggcaacg
     5401 ctgtactggt ttaaatttaa accactataa aatcaatttc accgaaaata tgccgccgca
     5461 catcaggcac agcacctccg accgccacca aacgcgggaa aaccatttcc cccttccttt
     5521 cacgctgccg cgttcaaaca cgccaagatc gaacaaagcg cgaacaaagc cgccaaaata
     5581 tgccataata gccgcgcaca ttccccgcgc aatgtgttcc aaaagaaaaa accaacctac
     5641 aagagaataa tcctatgact caaaaatcca ccattgttta tacccatacc gacgaagccc
     5701 ccgcgctggc gacccaatcg ctgctgccga ttgtgcaggc ttttgcccgc cacgccgata
     5761 ttgatgtcaa aactgccgac atttccctgt ccggccgtat tttggcggca ttccccgaat
     5821 atctgaccga agcgcaacgc gtacccgacg cgcttgccga attgggcgaa ctggtaaaac
     5881 aacccgatgc aaacgtaatc aaactgccga acatcagcgc atccgtaccg caactgaccg
     5941 ccgcgattaa agaattgcag tctaaaggct ttgccgttcc cgattatcct gccgacccgc
     6001 aaaccgatga agaaaaagcc gtgcgcgaac gctacgaccg catcaaaggc agcgcggtaa
     6061 accctgtcct gcgtgaaggc aactccgacc gtcgcgcccc caaagcggtg aaaaactttg
     6121 ccaaaaaaaa tccgcacagc atgggcgcat ggacaaaaga ctccaaaacc cacgttgcca
     6181 ccatgcaaag cggcgacttt ttccataacg aacaatccgt taccgtacct gaagcgactt
     6241 ccgtatccat cgtgttcacc gacaaacaag gcaataaaaa agagctgcgc gcgcctgttg
     6301 ccctgaaagc cggcgaaatc atcgacgcaa ccgtgatgag caaaaaagcc ctgctcgcct
     6361 tccttgccga acaagtgaaa gacgcgaagg caaaaggcgt attgttctca ctgcatatga
     6421 aagccacgat gatgaaagtg tccgacccga ttatcttcgg acacgccgtc aaagtgttct
     6481 tcgcgcctgt atttgaaaaa ttcggcggca aactggctgc cgcaggtgtc aacgtcaaca
     6541 acggcttcgg caacctgctg gccaatctgg acaaactgga tgcggacacc cgcgccgccg
     6601 tcgaagctga aatcgccgcc gtttacgccg ccaaccctga tttggcgatg gtcgattccg
     6661 ataaaggcat taccaacctg cacgttccca gcgacgtcat cgtcgatgcc tctatgcctg
     6721 cgatgattcg caattccggc cggatgtggg acaaagacgg caaagcgcaa gacaccaaag
     6781 ccgtcattcc cgacagcagc tatgccggtg tttaccaagc aaccatcgat ttctgccgcg
     6841 aacacggcgc attcgatccg acaactatgg gtaccgtgcc caacgtcggt ctgatggcgc
     6901 aggcggccga agaatacggt tcgcacaata aaacttttga aatcgaagcc gacggtcaag
     6961 tccaagtcat tgatgcggca ggaaaagtcc taatgcagca cgacgttgaa gccggcgaca
     7021 tctggcgtat gtgccaaacc aaagacgctc cggttaaaga ctgggtacaa cttgccgtca
     7081 accgcgcccg tctgagcaac acgcctgccg tgttctggct cgacgaaaac cgtccgcacg
     7141 acaagagcct gctcgccaag gttaaagctt accttgccga actggatacc gacggcctcg
     7201 acatccgcgt cctcgcgcct gaagaagccg ccaagttcag cttgggccgt ctgaaaaacg
     7261 gcgaagacac catctccgta accggtaatg tcttgcgcga ctacctgacc gacctgttcc
     7321 cgattttgga actcggcaca agcgcgaaaa tgctgtctat cgttccattg atgaatggcg
     7381 gcggtatgtt tgaaaccggt gcgggcggtt ctgcaccgaa acatgttcaa caattcctcg
     7441 aagaaaacca tttgcgctgg gactcgctgg gcgaattcct cgcactcgcc gtatcgtttg
     7501 aacatttggc gcaaaaaacc ggcaatgcca aagcccaagt cctcgccgac actttggatg
     7561 cagccaccga aaaactgctg ttgaacgaca aatcgcctaa acgtaaagcc ggcgaactcg
     7621 acaaccgcgg cagccatttc tacctcaccc tctactgggc gcaagaattg gcggcgcaag
     7681 acaaagatgc cgaactgaaa gccgcattta cgccattggc agccgctttg accgccgacg
     7741 aagcgaaaat cgttgccgag ctttccgccg tacaaggcaa agcggccgac atcggcggct
     7801 actacgccgc caatcctgaa aaagccgcac aagtgatgcg tccgagcgta acctttaatc
     7861 aggcactaaa cgccttataa gcacaaaggt aaaaatgccg tctgaacatt cgtcgttcag
     7921 acggcattag ttatccctat ctgcctgatt atgattaaac gccgcaccca ttggatat
//
LOCUS       DAWFJR010000088         7963 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWFJR010000088 DAWFJR010000000
VERSION     DAWFJR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.088"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            366..665
                     /locus_tag="WGR04_001719"
     CDS             366..665
                     /locus_tag="WGR04_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0795232.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFLFAVLTVLKLKGYQAGLYTLLIALAVAAFGFGMPAGMAVSSLPPQPH"
     gene            605..841
                     /locus_tag="WGR04_001720"
     CDS             605..841
                     /locus_tag="WGR04_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0795233.1"
                     /translation="MRLRDAGGYGGFFPAAAAALTQRPYATLDLTAHYKIGKSTRIGL
                     DFENVFNKRYRPMPDIHVYGTPRSLTATVKHIVD"
     gene            complement(1033..2436)
                     /gene="mtrE"
                     /locus_tag="WGR04_001721"
     CDS             complement(1033..2436)
                     /gene="mtrE"
                     /locus_tag="WGR04_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ0795234.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVDTHSAELGGLFKSGTGVWL
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALTAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(2491..5694)
                     /gene="mtrD"
                     /locus_tag="WGR04_001722"
     CDS             complement(2491..5694)
                     /gene="mtrD"
                     /locus_tag="WGR04_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ0795235.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(5706..6944)
                     /gene="mtrC"
                     /locus_tag="WGR04_001723"
     CDS             complement(5706..6944)
                     /gene="mtrC"
                     /locus_tag="WGR04_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ0795236.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIASITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
     gene            7195..7827
                     /gene="mtrR"
                     /locus_tag="WGR04_001724"
     CDS             7195..7827
                     /gene="mtrR"
                     /locus_tag="WGR04_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ0795237.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDTEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT         1908 a         2204 c         2046 g         1805 t
ORIGIN      
        1 ccgtactggt ttttgttcat ccactataca tcaaacatca aattggactg accaatcagg
       61 gcggattcta atgacacgcc gttcccgccg tcaacggcat ttacctcgca ccgcccccga
      121 aacacaggaa aaaactacac caaactacaa tttttgttta tgcaaatatt tgttttgaca
      181 ggatttaaac gaaagctccg attcaaatct gccgaaccgc ccaaaaatat atagtggatt
      241 aacaaaaatc aggacaaggc gacgaagccg cagacagtac agatagtacg gtaaggcgag
      301 gcaacgccgt actggtttaa atttaatcca ctctattgac ctgaatatta aagtttcgca
      361 aagtaatgca acgttgcttt aattggtttg accactattg ccgacgatta gaaaaatatt
      421 ttcggagatg ttcaattatg gaaacttggg ttcaaaacta cacggcaatc ggcggcagcc
      481 tgtatctgac tgccgccgcc gcactcttac ccatcgtctt tttatttgcc gtgctgaccg
      541 tcctgaagct gaaaggctat caggcggggc tttatacgct gctgattgcg cttgccgttg
      601 ccgcattcgg cttcgggatg ccggcgggta tggcggtttc ttccctgccg ccgcagccgc
      661 actgacccaa cgcccctacg ccacgctgga tttgaccgcg cattacaaaa tcggcaaatc
      721 cacccgcatc ggtttggact ttgaaaacgt gttcaacaaa cgctaccgcc ctatgcccga
      781 cattcacgtt tacggcacgc cgcgcagcct gaccgcaacc gtcaaacata tagtggatta
      841 acaaaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacaa cttggtgctt
      901 cagcacctta gagaatcgtt ctctttgagc taaggcgagc caacgctgta ctggtttttg
      961 ttaatccact ataaagaaag aaatgccgtc tgaaacctta tcgtttcaga cggcatttta
     1021 ttgcccgacc ggttatttgt cggtttgggt atcccgtttc aatccgccgc cgagtgcctt
     1081 gtacaaatcg gcaaggtttt cggcgcgggt cagttgtgcc gacaaagccg caccttccgc
     1141 cgaatagctg ctgcgttccg catcgagcaa atcaagcgcg ccggatacgc cgtgtttgta
     1201 acgcagaccg accaaacgca acgcttcttt agaggcgcgg ctttgtttgc ttaaagcatc
     1261 ataggcttta tccaactgct cgcgcgcggt caatgcgttt gccacgtctt gaaatgcgga
     1321 ttggacggcg gattcatagg caacgatttg tgcctgttgg cgcagttttg ccacatcgag
     1381 gttcgccttg ttcgtacccc aggtaaaaat cggcagggta atggaaggtg cgaacaacca
     1441 aacgccggtg ccgcttttga acagcccgcc caattcggca gaatgcgtat cgacgcttcc
     1501 ggtcaggcgg atggatggga aaaaggcggc gcgtgccgca ccgatattgg cgtttgcctg
     1561 tttgagcgcg tgttcggcag cacggatatc gggacggtcg agcaatactt cggaactcaa
     1621 accggccggc agcttctcaa caaaaaactg cttgtccaac ggcaaaccgg cgggcaggtc
     1681 gtcgggtatc ggttggttaa tcagggttgc caaggcattg cgcgcctgtt cgcggctgcg
     1741 cgcggcatgg gcataatcgg ctttggcaga ttcgattagg gcttcctgct gacgtagggc
     1801 gacggcggaa atcacgcctg ccttgtaacg taattcggac agcttgtagg tttcctcgcg
     1861 cgttttcaaa acacgttgcg ccaaagacat cgcttcttcg gcataacgtt cgttgaaata
     1921 ggctttggca acggtggcaa tcaggctcaa atgtgccgca tcgcggttgg cggtgctggc
     1981 gaaatagcct tgcagtgccg cctcgctgct gctgcgtaca cgcccgaaca gatcgagttc
     2041 gtaagatgcc gcacccagtc cgactttgta gctgctgctt acattgccgc cgctcaagct
     2101 gccttggcgc gagtcgttcg cattggcggc aagcgtgggc aggaggttgt tgcgctcaat
     2161 catgtattgt ttgcggtaga tttcgctgtt caatacggcg gtacgcaaac tggtattgcg
     2221 ctcgagtgcg atgtcgatca gcttttgcag gcgcgggtcg gcaaaatagt cgtgccaacc
     2281 caaatcgacg gcgcggatgc cgctgtcggc ggtatcgttt ttgaacgttt cggcaacttc
     2341 gactttgggc tgctcgtatt ggggaatcat ggtgcaggca gacaatgcga aggctgctgc
     2401 aacagaagtc aaggtggttt tcaatgtagt atccataaaa aagtcctgat gccgtctgaa
     2461 aacccgtggg cgttcagacg gcatggttgc ttaatgttgg ctgtcgtccg aaccggtgat
     2521 gcccgcttcg gcggcgtgtt ttactgccat ttcgtgttcg tgcgcggttt ctttgaagaa
     2581 tttgcgcacc accacataga aaagcggaac aaggaacacg gacaagagcg tgccgatgag
     2641 catcccccag aatacggttg taccaatggc gcgctggctg gcagaacttg caccgccggc
     2701 aatatacagg ggaaccacgc ccaaaataaa ggcgaacgag gtcatgataa tcggacggaa
     2761 acgcaggcgg gcggcttcca aagcagcttc gaccgcgctt ttcccttgcg cttgaaggtc
     2821 tttggcaaat tcgataatca aaatcgcgtt tttcgcactc aaacccatca cggtaacgaa
     2881 accgacttga aagtagatgt cgttggcgaa cgagggaacg ctgcccaaca gtccttcaaa
     2941 caggttgcgc ccggttacgc ccgcagccgc accgatcaaa cccaacggaa tcacaaggat
     3001 gaccgccagc ggaatcgacc agctttcata aagcgcggca agtaccaaaa atacggctgc
     3061 aaccgccaaa ccgtacaaaa tcagggtttg cgagccgcct tttgcctctt cgcgcgactg
     3121 tccgccccat tcgaagctgt aaccgccgcc caattcgtca accatttttt gaaccgccgc
     3181 catagcctgc ccggtggaaa cgccggttgc aggcgaagcg gacagcttca tcgaaggata
     3241 accgttgaag cgtacgctct gttccgtacc gttttcccaa gaaacagtag caatggtgga
     3301 aagcggtacg gcgacgccgg atttgttcgg cacggtcagg ttcaaaatat cggcaggctg
     3361 catacgggca tcctcgtcgg cctgcaccat cacgcgttgc agacggcctt ggttcgggaa
     3421 gtcgctgaca taagacgaac tcagcgcgct tgccaatgcg gtgcggatgt cggcaaacga
     3481 aatgccttgc gccgccgcgg cggcacggtt gatgtcgatt ttcaactgcg gcgagtcttc
     3541 caaaccgccg gcacggacgg tgctggggtc aaacaaaccg ctggcacgca ttttctgaat
     3601 caactcgttg cgcttcgcca gcaatgcggt atggccggta ttgttgcggt cttgcaggtt
     3661 gatgctcagg cccgaaccat tgcccaactc cagaatcgga ggcgggacga cggcgatgcc
     3721 aaaaccgtct ttaagcgtcc ccatcatcat acccgtcagc ttgccggcaa tcgcaacggc
     3781 atcgctgccg ggcgcggtac gctcgttcca atctttcaat atggcaaaac ccatcgccat
     3841 attctgaccg ctgcccgaaa agctgaagcc ggaaacggta atgatgtttt ctatttcagg
     3901 aatgcttttc gccagttggg taacttgcgc caaagtcgca ttggtgcgct cttgggtcgc
     3961 tcctgcaggc agttgcacgc tgaccatgac gaagccttgg tcttcggtcg gcaggaatga
     4021 agtcggcagg cgcataaaca ggaacacgcc cacaaccgcc aagccgatat agacaaccat
     4081 catgcggaaa gtcttacgca gcactttggc aacccggcct tcgtaaccgt gcgtccaact
     4141 gttgaatttc ttgttaaacc agccgaagaa accttttttc tcttcgtgat gccctttcgg
     4201 gattgtcttc aacattgtgg cacacaaagc aggggtaagg gtcagcgcaa ggaaggcgga
     4261 gaatgcgatt gatgacgcca tcgtcagggc aaactgtttg taaatattgc ccgtcgcccc
     4321 gctgaacatc gccaacggta cgaacacgga aatcagaacg gcggtaatac cgatgaccgc
     4381 gcccgaaatc tgacccatcg cttttttggt cgcttctttg ggcggcaagc cttcacccgc
     4441 cataatgcgc tcgacgtttt caaccaccac aatcgcgtca tcgaccacga tgccgatgac
     4501 caaaaccatc gcaaacatgg tcagtacgtt aatcgacatg cccatataag agatgaaggc
     4561 gaaaccgccc aacagcgaaa tcggtacgac gatggtcgga atcagcgtat aacggatgtt
     4621 ttgcaggaag agatacatta cgacaaacac cagcaccatc gcttcgatta aagtatgaat
     4681 cactttttca atcgaaattt cgacgaattt ggaagtatcg taaggggttt tccagctcat
     4741 accctgagga aagtattttt ccaacgtcgc catgcgttct ttaaccgcct ttgccgtcgc
     4801 catcgcattg ccgctgttgg acagcatcac cgccataccg gtggtattta caccgttcag
     4861 acgggttgag gaagaatagt cttccatacc cagtccgacc cttgccacat ccttcaggta
     4921 aacattagaa ccgtcggtat tggcgcggag gatgacgttg ccgaattctt ctgccgtacc
     4981 caactgccct tgcgccgtta cggtagccgt aaccgtctgt ccgcgaacgg cgggaagcga
     5041 accgatagaa cccgctgaaa tctggacgtt ctgggcggac agcgcgctgc cgatatcggc
     5101 aaacgacaaa ttgtagtttt gcagtttctt aggatcaacc caaatccgca tcgcgcgttg
     5161 cgcgccgaac aggcgtacct gccccacgcc ttcgatacgc tgcaactcgg gaacgatatt
     5221 acgctgcgcg tagtcgttca tctcttcggt tgactgcaca tccgacgaaa gcatcacaat
     5281 catcaggaaa ttggaacgcg ccttggatac ggttacgccg tattgctgga cggttgccgg
     5341 cagcgtgctc aatacttcgg aaagcttgtt ctgcacttcc acctgcgcca gattctcgtc
     5401 ggtatcgggc gtaaaggtca ggctcacgct gccgctgccg ctcgaatcgg cggaagtgga
     5461 catataatcc aaaccttcca cgccgttcat attccgctcg atcacggaaa gcacgctgtc
     5521 ttccattacc tgcgcggacg cacccggata agtggcgtgc aaagtaatgg tcggggcggc
     5581 gacagacgga tattgcgaaa caggcaggct tttgatgccg aaaatacccg ccgcaataat
     5641 gaaaatcgaa ataacccacg caaaaatagg gcggtcgata aaaaatttag ccatcgatgc
     5701 cttccttatt ccgcttcaga agcagttttg gcttcagatg ccgtctgaac gccggattga
     5761 ggcgcggcgg cttggttttc agacggcgcc cattctttgg gcgttacctt tttcgcaccc
     5821 gttatactgg cgatactgat gccttccaca accaccttgt ccccgtcctt cagacccgac
     5881 gtaacaatcc aattcgtacc ctgctgttgc gcaaccgtta cctcgcgggg ttccataccg
     5941 ccttgggcat tcacaatcat cacggtatct ttcgcaccgc gcgttaccgc ctgctgcggc
     6001 acaacaaatg cgttatccac cgccacttgg tccatcagca cgcgcacata caggccgggc
     6061 atcaggatat tctgatcgtt cggtacggcg gcgcgcaggg taatctgacc ggtcgattcg
     6121 ttgacggtcg gatcggcaaa cagcaggcgg cctttttcag ggtaaactgt gccgtcgtca
     6181 aatttgatgc cgaccgcaat cacaccatcc gccgccagca gtttgccttc ggctatctga
     6241 cggcgcaatt tcatcacttc ggatgcagac tgggtaacgt tcacatacat cggattggtt
     6301 tggcggatgg ttgccagcac ggtcgtatcg cccgcattca acagcgtacc ttcggaaact
     6361 ttggactgac cgataaagcc ggaaatcggc gcggtaatgc gcgaacggtt cagattgatg
     6421 ccggcagatt tgatcgccgc ctgtgctgct ttgacacctg cctcggcaga acgtttcgcc
     6481 gttaccgcag catcgtattc ctgccggctg acggcttcgg cggcaaccaa aggcttgtat
     6541 cgcgccaaat ccgcatccgc tttggcaagc gttgcctgag ccgttgccag ttgcgcgcgc
     6601 gcgctttcca gacctgcttc ataagtggaa ctgtcgatct gatacagcgg ctgtccggca
     6661 cggacataac tgccttcttg gaacaggcgt ttttggatga tgccgccgac ttgggcgcgg
     6721 acatcggcgg tacgcagcga ttccaaacgc cccggcaact cgacggtcaa tgcgacggtt
     6781 tgcggatgga cggttacgac accgacgacg ggcgcagggg cttcccgacc agcaggctgc
     6841 ccgccctgcg ccgcgtctcc gcctttaccg caagacgaca gtaccaatgc aacggcggca
     6901 gccaacgcgg ccgcacgcat cgccttaaaa gcataaaaag ccattattta tcctatctgt
     6961 ctggtttgat gtaaagggtt ttgccaatca acaggcattc ttatttcagg atataaaaac
     7021 cgcctgcttc gccaacccga tgttcaaacg gattgcgaag caggtttcat gggttttcaa
     7081 agttgagatg tagtctcaat ttcaggggtg ttattataca tacacgattg catggttaca
     7141 aagtcttttt tataatccgc cctcatcaaa ccgacccgaa acgaaaacgc cattatgaga
     7201 aaaaccaaaa ccgaagcctt gaaaaccaaa gaacacctga tgcttgccgc cttggaaacc
     7261 ttttaccgca aagggattgc gcgcacctcg ctcaacgaaa tcgcccaagc cgccggcgta
     7321 acgcgcggcg cgctctattg gcatttcaaa aataaggaag acttgttcga cgcgctgttc
     7381 caacgtatct gcgacgacat cgaaaactgc atcgcgcaag ataccgaaga tgccgaagga
     7441 gggtcttggg cggtattccg ccacacgctg ctgcactttt tcgagcggct gcaaagcaac
     7501 gacatctact acaaattcca caacatcctg tttttaaaat gcgaacacac ggagcaaaac
     7561 gccgccgtta tcgccattgc ccgcaagcat caggcaatct ggcgcgagaa aattaccgcc
     7621 gttttgaccg aagcggtgga aaatcaggat ttggctgacg atttggacaa ggaaacggca
     7681 gtcatcttca tcaaatcaac cttggacggg ctgatttggc ggtggttctc ttcctgcgaa
     7741 cgtttcgatt tgggcaaaac cgccccgcgc atcatcggga taatgatgga caacttggaa
     7801 aaccatcccg acctgcgccg gaaataatca agccttggta acaatgccgt ctgaaacgaa
     7861 caaacccttt cagacggcat caaaatgaca caaagccttc ttctaaaaat acatattgag
     7921 acctttgcaa taacataggt tactaaaatt ttatgctcaa tct
//
LOCUS       DAWFJR010000089         7962 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWFJR010000089 DAWFJR010000000
VERSION     DAWFJR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7962
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.089"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(14..430)
                     /gene="dksA"
                     /locus_tag="WGR04_001725"
     CDS             complement(14..430)
                     /gene="dksA"
                     /locus_tag="WGR04_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ0795238.1"
                     /translation="MAKLTEQDILNWNGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(575..1366)
                     /locus_tag="WGR04_001726"
     CDS             complement(575..1366)
                     /locus_tag="WGR04_001726"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ0795239.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRERLGKEL
                     GVETSATLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVDTLSRYLGG
                     TRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDCKIADRIMKSVGLTIWLEDEAQMHGI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAINEGVCACVRRSQEMERQYQ"
     gene            complement(1415..1816)
                     /locus_tag="WGR04_001727"
     CDS             complement(1415..1816)
                     /locus_tag="WGR04_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ0795240.1"
                     /translation="MRAIKHLAMALLLMFTVAGCQLAGWYKCSSLSGWCKPRKPAAID
                     FWDIVGESPPSLEDYEIPLSDGNRSVRVNEYESAQQSYFYRKIGKFEACGLDWRRRDG
                     KPLIETFKQEGFDCLKKQGLRRNGLSERVRW"
     gene            complement(1821..2507)
                     /locus_tag="WGR04_001728"
     CDS             complement(1821..2507)
                     /locus_tag="WGR04_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ0795241.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQAGREPHSVSLIAVSKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAAKHGVAPEEAVVLAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNAAGVKADVLSMGMSDDMPAAIACGATHVR
                     IGSAIFGKRG"
     gene            2627..3670
                     /locus_tag="WGR04_001729"
     CDS             2627..3670
                     /locus_tag="WGR04_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ0795242.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNIGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3833..5059
                     /locus_tag="WGR04_001730"
     CDS             3833..5059
                     /locus_tag="WGR04_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0795243.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5345..7495
                     /gene="yccS"
                     /locus_tag="WGR04_001731"
     CDS             5345..7495
                     /gene="yccS"
                     /locus_tag="WGR04_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ0795244.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDNPDIRHLRRLLDNLGS
                     VDQQFRQLRHSDSPAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVRSSGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1970 a         2622 c         1723 g         1647 t
ORIGIN      
        1 ttgtttgccg ccatcagtcg gcaaactgtt ttttcatcct ctctcggcgt tcttgggact
       61 caacagataa agtggctgtc gggcgtgcca gcagccgctt cagaccgata ggctctcccg
      121 tatcggcaca gaatccataa tccccttcat caatattgcg gatggtcgcc tgtattttac
      181 tgagaagttt tcgttcccga tcgcgggtac ggagttccaa tgcgtactct tcttcctgtg
      241 tggcacggtc ggcaggatcg ggggctgatt cgtgttcttg gagatgccct gtcgtagcgg
      301 aagcattttc gatgagttca tcttgcattt taaccagcaa ttcacgaaaa aaagccaaat
      361 ggtcgtcatt catataatcg tcttccggcc cattccaatt caaaatatct tgttctgtca
      421 gctttgccat aatgtatgtc ctctcagttc accgaaaaaa aagcaccaca aagtggcttt
      481 attcctttga tttttataag gcttattgcc taaggcgttt ttgaaaacgc gcatcataac
      541 acgttttgga ttggtttttt ttattttctc tacattattg atattgccgt tccatttcct
      601 gcgaacggcg cacacaggca caaacgcctt catttatggc ttcggcgaca cgatgccgtc
      661 tgaaggcttc cacggcttcg tgagttgtcc cgccttttga cgttacattt ttttgaagct
      721 tctcgaaatc ttcaccagtc tgctcggcaa gggcaaccgc tcctttaaat gttgccagac
      781 tgagcgcgcg tgcttctgcc atatcaaacc cttgtcggat ggcggcattt tgcaatgcgt
      841 ccagcagata aaacacataa gccggcccgc tgccgctgat gccggtaatg ccgtgcattt
      901 gcgcttcatc ttccaaccaa atggtcaaac cgacagattt catgattcga tcggcaatct
      961 tgcagtctgt ttccgatact tccgcttcgg catacatacc ggatacgccc agcccgattt
     1021 ttccgggtgt attcggcata acccggacaa tgcggcgtgt tcccccgagg taacggctga
     1081 gcgtatcaac cgacaatcca gctgcgacag aaagcaccaa tgcaccattg gtgcggatat
     1141 ttttgcacgc ggcttccata tcctgcggct tgacggcaag gattaaaaca tcgtcggaat
     1201 gaagctccgg cagggttgcc gaagtttcga cccccaactc ttttcccaaa cgttcgcgtt
     1261 tttccgcacc ccgattggca atatggatgc ggtaaccgcc ttgtttgacc aatccgcccg
     1321 caacggcagc cgccatattg ccgccgccga gaaaataaac attcattatt tctaccctat
     1381 tcaaaattac ctgactaaat tcattcccaa ttttttacca tcggacgcgc tcggacagac
     1441 cgttgcgccg caacccctgc tttttcaaac aatcaaaacc ttcctgtttg aacgtctcaa
     1501 tcaaaggttt gccgtcacgc ctacgccaat ccaacccgca ggcttcaaac ttccctattt
     1561 tcctgtaaaa gtaagattgt tgtgcggatt catattcgtt taccctgacg gaacgattgc
     1621 cgtctgaaag cggtatctcg tagtcctcta aagacggcgg actctcgccg acaatatccc
     1681 aaaaatcgat ggcggcaggt tttctcggct tacaccagcc ggacagggac gaacacttat
     1741 accaacctgc caactgacag ccggctaccg taaacataag caataacgcc attgcaagat
     1801 gttttattgc gcgcattcca tcagcccctt ttcccgaaaa tcgcgctgcc gatacggaca
     1861 tgtgttgcac cgcacgcaat ggcggcaggc atatcgtccg acatccccat agacagcacg
     1921 tccgccttaa cgccagccgc attgaggtcg gcaagcagtt tccgcatcgt ttgaaattgc
     1981 accttcaact ccgtttcact gctgttggct ttggcaacac acatcaagcc gcgtacgacg
     2041 atattcggca gcttcgccac ttcgaccgca agcacgaccg cttcttcggg cgcgacaccg
     2101 tgttttgccg cctcgcccgc aatgttcacc tcgatacaca cctgcaaagg cggcattgag
     2161 gaaggacgtt gcccgctcag ccggacggcg gttttcagac ggcatacggt atgcacccaa
     2221 tgcgcgcgtt cggcgacaaa tttggttttg ttggactgca catcgccgat gacgtgccac
     2281 acgatgtcgg tcaaatccgc caactcttcc gttttgccgt accactcctg aatatagttc
     2341 tcgccgaaat cacgctgtcc ggcggcgtaa acttcgcgga tgccgtctga agggaaagtc
     2401 ttactgacgg caatcaggct gacggaatgc ggctccctgc ccgcctgcag aaccaatttt
     2461 ccgatacggt cggacacctc acaataacgt tcttgcaaca ccgtcataga ttatccccta
     2521 attaaaaatg attaaacagt tgaaacccct ccagtcaggg cggtacaatc aaggttgtta
     2581 gaaccattcc aaccaatcga aacattatac taaacagagc cgcattatgc agattaccga
     2641 cttactcgcc ttcggcgcta aaaacaaagc atccgacctt cacctgagtt cgggcatatc
     2701 ccctatgatt cgggttcacg gcgatatgcg gcgcatcaac cttcccgaaa tgagcgcgga
     2761 agaggtcggt aatatggtaa cttcggtgat gaacgaccac cagcggaaaa tctaccagca
     2821 aaacttggaa gtcgacttct cgtttgaact gcccaacgtc gcccgattcc gcgtcaacgc
     2881 cttcaacatc ggccgcggtc ccgccgccgt attccgcacc attcccagca ccgtcttatc
     2941 gctggaagaa ttgaaagccc cgagcatttt ccaaaaaatc gcagaatcgc cgcgcggcat
     3001 ggtattggtt accggcccta ccggttcggg caaatcgacc acgcttgccg cgatgatcaa
     3061 ctacatcaac gaaacccagc cggcacacat cctgaccatc gaagacccga tcgaattcgt
     3121 ccaccaaagc aaaaaatccc tgattaacca gcgcgagctg caccagcaca ccctcagttt
     3181 cgccaacgcg ctgcgttccg cattgcgcga agaccccgac gttatccttg tcggcgagat
     3241 gcgcgacccc gaaaccatcg gcttggcgct gaccgccgcc gaaaccggac acttggtttt
     3301 cggcacgctg cacacgaccg gcgcagcaaa aaccgtcgac cgtatcgtgg acgtattccc
     3361 ggcgggagaa aaagaaatgg tgcgctctat gctgtccgaa tcgctgaccg ccgtcatctc
     3421 ccaaaacctg ctgaaaacgc acgacggcaa cggccgtgtc gcctcgcacg aaatcctgat
     3481 tgccaacccc gccgtccgca acctcatccg cgaaaacaaa atcacgcaga ttaactccgt
     3541 cctgcaaacc gggcaggcga gcggtatgca gacaatggac caatcgctgc aatcgctggt
     3601 gcgccaaggg ctgatcgcac cggaagtcgc acgcagacgc gcgcaaaaca gcgaaagtat
     3661 gagtttctga cacacaaccg ccttccggcc ataccggcgg gaaaacaagg cgcaaacacg
     3721 cgaggcggga cgcagcatcc cgcccggcta cctttccgaa caagaagcgt ccgccttcct
     3781 gttgaaacct gccgccgcaa actgcaaggc ttaaaccgaa aagaagctaa cgatgaatac
     3841 cgataacctg cacgacatct tggacgaaat ggttcaagtg tattctcaaa aaaaacaaag
     3901 ccgatccgaa accccggccg aaatcggcgc acacttccac ccgctgctcg accgcctgtg
     3961 cgaaaccgca gaagcacaaa acgcgtccga catccttatc agcaaaggat tcccgccctc
     4021 gttgaaaatc aacagcgcat taaccccgca gccgcaaaag gcgctgacag gcgaggaaac
     4081 cgccgccatc gccgcatcga cgatgaacgc cgaacaatcg gaaatattcc ggcgcgacgg
     4141 cgaaatcaac tactccgtcc agtcgcgcag cggcacgcgc taccgcgcca acgcctacca
     4201 cagccaaggc agcgcaggtt tggttttgcg gcgcatcaac cacgtcatcc cgcaaatgca
     4261 ggaattgggc ctgcccgaaa aactcaaaga cctcgccgtc gcaccgcgcg ggctgctgat
     4321 tatcgtcggg cctaccggtt cgggcaaatc caccacgatg gcgactatgc tcgaacaccg
     4381 caacaaaacc ctgcccagcc atatcgttac catcgaagac ccgattgagt ttatctacaa
     4441 accgcgccgc tgcattttta cccagcgcga aatcggcgtc gacaccataa actggcagac
     4501 ggcggtacaa aacgctatgc gccaatcccc cgacgtggtc tgcatcggcg aagtccgcag
     4561 cagggaaagt atggaatacg cgatgcagct cgcccaaacc ggccacctgt gcatttttac
     4621 gctccacgcc aacaccgcgc cgcagtcgct cgaacgcata ctcaacttct accccaaaga
     4681 acagcacaac caaatactga tcgacatcgc cctcaacctg accggcatca tctgccaacg
     4741 cctcgccctc aaacaagaca aaacgggcag gacggcggtt gtcgacttgc tcatcaacac
     4801 gcccgccatc caagacttca tcctgaaggg cgacctgatg aacatcagta aaatcatgga
     4861 aaccgccaaa accgacggaa tgcagacgat ggatcaaaac cttttcgaac tgtaccgtca
     4921 cggcatcatc agttacgaag aagccctgcg ccagtccgtt tccgccaaca acctgcgatt
     4981 gcacatccaa ctgcacaaag aaggcaaaac gcccgaactc ctttacgaca gggtcaacgg
     5041 tctcaacctc atttcctgat ccgcaaaacc caatgccgtc tgaaaaccgc atccccgttt
     5101 tcagacggca tgattttatc cgccccattc atgtgctaca ctttatagtg gattaaattt
     5161 atagtggatt aacaaaaatc aggacaagac gacgaagccg cagacagcac aaatagtacg
     5221 gaaccgattc acttggtgct tcagcacctt agagaatcgt tctctttcag ctaaggcgag
     5281 gcaacgccgt actggttttt gttaatccac tatattcact tcataacaat aaaccggtaa
     5341 aaccatgaaa accccactcc tcaagcctct gctcattacc tcgcttcccg ttttcgccag
     5401 tgtttttacc gccgcctcca tcgtctggca gctaggcgaa cccaagctcg ccatgccctt
     5461 cgtactcggc atcatcgccg gcggccttgt cgatttggac aaccgcctga ccggacggct
     5521 gaaaaacatc atcgccaccg tcgccctgtt taccctctcc tcgctcacgg cacaaagtac
     5581 cctcggcaca gggctgccct tcatcctcgc catgaccctg atgaccttcg gctttaccat
     5641 tttaggcgcg gtcgggctga aataccgcac cttcgccttc ggcgcactcg ccgtcgccac
     5701 ctacaccaca cttacctaca cccccgaaac ctactggctg accaacccct tcatgatttt
     5761 gtgcggcacc gtactgtaca gcaccgccat catcctgttc caaatcatcc tgccccaccg
     5821 ccccgtccaa gaaagcgtcg ccaacgccta cgacgcactc ggtggctacc tcgaagccaa
     5881 agccgacttc ttcgaccccg atgaggcagc ctggataggc aaccgccaca tcgacctcgc
     5941 catgagcaac accggcgtca tcaccgcctt caaccaatgc cgttccgccc tgttttaccg
     6001 tttgcgcggc aaacaccgcc acccgcgcac cgccaaaatg ctgcgctact acttcgccgc
     6061 ccaagacata cacgaacgta tcagctccgc ccacgtcgac taccaagaga tgtccgaaaa
     6121 attcaaaaac accgacatca tcttccgcat ccaccgcctg ctcgaaatgc agggacaagc
     6181 ctgccgcaac accgcccaag ccctgcgcgc aagcaaagac tacgtttaca gcaaacgcct
     6241 cggccgcgcc atcgaaggct gccgccagtc gctgcgcctc ctttcagaca gcaacgacaa
     6301 tcccgacatc cgccacctgc gccgccttct cgacaacctc ggcagcgtcg accagcagtt
     6361 ccgccaactc cgacacagcg actcccccgc cgaaaacgac cgcatgggcg acacccgcat
     6421 cgccgccctc gaaaccggca gcctcaaaaa cacctggcag gcaatccgtc cgcagctaaa
     6481 cctcgaatca ggcgtattcc gccatgccgt ccgcctgtcc cttgtcgttg ccgccgcctg
     6541 caccatcgtc gaagccctca acctcaacct cggctactgg atactgctga ccgccctttt
     6601 cgtctgccag cctaactaca ccgccaccaa aagccgcgtc cgccagcgca ttgccggtac
     6661 cgtactcggc gtaatcgtcg gctcgctcgt cccctacttc accccgtctg tcgaaaccaa
     6721 actctggatt gtcatcgcca gtaccaccct ctttttcatg acccgcacct acaaatacag
     6781 tttctccacc ttcttcatta ccattcaagc cctgaccagc ctctccctcg caggtttgga
     6841 cgtatacgcc gccatgcccg tacgcatcat cgacaccatt atcggcgcat cccttgcctg
     6901 ggcggcggtc agctacctgt ggcccgactg gaaatacctc acgctcgaac gcaccgccgc
     6961 ccttgccgta cgcagcagcg gcgcctatct cgaaaaaatc accgaacgcc tcaaaagcgg
     7021 cgaaacaggt gacgacgtcg aataccgcgc cacccgccgc cgcgcccacg aacacaccgc
     7081 cgccctcagc agtacccttt ccgacatgag cagcgaaccc gcaaaattcg ccgacagcct
     7141 gcaacccggc tttaccctgc tcaaaaccgg ctacgccctg accggctaca tctccgccct
     7201 cggcgcatac cgcagcgaaa tgcacgaaga atgcagcccc gactttaccg cacagttcca
     7261 cctcgccgcc gaacacaccg cccacatctt ccaacacctg cccgaaaccg aacccgacga
     7321 ctttcagaca gcactggata cactgcgcgg cgaactcgac accctccgca cccacagcag
     7381 cggaacacaa agccacatcc tcctccaaca gctccaactc atcgcccgac agctcgaacc
     7441 ctactaccgc gcctaccgcc aaattccgca caggcagccc caaaatgcag cctgaaaaag
     7501 tttcggcatt ttgtaagaga ggacagattg tcagacaggt tacaagatag tggataaagc
     7561 tttgcgcagg gtaaatgcgt agcaactgaa ccgtcattcc cacgaaccta catcccgtca
     7621 ttcccacgaa aacagaaaac caaaaacaga aacctaaaat cccgtcattt ccacgacaat
     7681 gggaatccag ttcgttcggt ttcgcttgtt ttaagtttcg ggtaacttcc acttcgtcat
     7741 tcccgcgcag gcgggaatcc agtgcgttga gcttcagcta tttagaataa attttgaaac
     7801 tctaatcccg tcattcccac gaacctacat tccgtcattc cctcaaaagt gggaatccaa
     7861 aatcccggac tttcagataa tctttgaata ttactgttgt tctaaggtct agattcccgc
     7921 ctgcgcggga atgacgaatc catccgcacg gaaacctgca cc
//
LOCUS       DAWFJR010000090         7879 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWFJR010000090 DAWFJR010000000
VERSION     DAWFJR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.090"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(130..1047)
                     /gene="thrB"
                     /locus_tag="WGR04_001732"
     CDS             complement(130..1047)
                     /gene="thrB"
                     /locus_tag="WGR04_001732"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ0795245.1"
                     /translation="MSVYTSVSDDEMRGFLDDYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACTRLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(1083..1811)
                     /gene="ubiG"
                     /locus_tag="WGR04_001733"
     CDS             complement(1083..1811)
                     /gene="ubiG"
                     /locus_tag="WGR04_001733"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ0795246.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1963..3204
                     /locus_tag="WGR04_001734"
     CDS             1963..3204
                     /locus_tag="WGR04_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ0795247.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSALGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKMPVLFDTQAPVGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNAFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            3354..4411
                     /locus_tag="WGR04_001735"
                     /pseudo
     CDS             3354..4411
                     /locus_tag="WGR04_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            4687..5250
                     /gene="gmhB"
                     /locus_tag="WGR04_001736"
     CDS             4687..5250
                     /gene="gmhB"
                     /locus_tag="WGR04_001736"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ0795248.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            5279..6022
                     /locus_tag="WGR04_001737"
     CDS             5279..6022
                     /locus_tag="WGR04_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0795249.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     GKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            6019..6711
                     /locus_tag="WGR04_001738"
     CDS             6019..6711
                     /locus_tag="WGR04_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0795250.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPAPSSLTSAKTFWGV
                     CRKTTGIRFNWRLVGAPEYVADYVCIHELCHLAHADHSRAFWALTRRFAPYTPEAKQW
                     LKIHGRELFALG"
BASE COUNT         1885 a         2536 c         1827 g         1631 t
ORIGIN      
        1 gtcctgattt ttgttaatcc actataaaat ggaaaatacc cggctatcgt ctcattttcg
       61 ttttaatcag ccataaaaat gccgtctgaa accctttcag acggcatttc tgtcaaacgc
      121 cggacgcact caatccaaac tcaacagcag gttgcggaac gcgttcgggt ctttgataaa
      181 cgtcatctcg cccgcctgcg gaaaatgaaa gtccaacagg cgcgacaccc aaaaacggat
      241 gcagccggca cgttgggcgg tcgggaaata cgccttttct tcggcactca aggggcgcac
      301 gccctcataa ccaccgataa acgccttttt caacgcctca tccaacttat tgtccgctgt
      361 ccttgcccaa tcgttgaccg caatcgccaa gtcatacata aaattgcccc ggcaggcata
      421 atagaaatcg atgaaacccg atacctgacc gccgtcaagc aacacattgt ctttaaacaa
      481 atcggcgtgg atgatgcccg aaggcagatg attgccgaga ttgtccttca gcgcatcgat
      541 ttcggaacac agcagcgcgg catcgtcttg cgacaggacg ggcagcagcc gggtgcacgc
      601 ctccgtccac cacgcatcgt aacgcgggtt ttccatttcc aaagggaaat cggcggcggc
      661 aaggtgcatt ttggccagca tcgcaccggt atggaaacac tgctcagccg tcggcagcgc
      721 ggtatccgaa cctttcaggc aggcaaccaa acaagccggc ttgcccgcca aaacggaatc
      781 aagccggccg tctttgcgcg caaccggcgc ggcaaccgcc acgcccttca tactcaaatg
      841 ccggttaagc tccagaaaaa acggcagctc ttcctgtttc aacacttcaa acacggtcag
      901 cacataacgt cccgaagtcg tcgtcagaaa ataattgctg ttggtaatcc cctgcgcgat
      961 gccctgcagg gaaacaaatt ctcccaaatc gtaatcatcc aaaaagccgc gcatttcatc
     1021 atcggaaaca ctggtataaa cagacatatc cattcacccg ttcaaaacaa tcaaacccgc
     1081 cgtcagaacg ccggacggca ggcaaacata taattcacat cggtcgaatc gcacagggca
     1141 taagtttgcg acaacacatg gtaagtcata cccttcgtat ccgccacatc caagcctgcc
     1201 tgacggcaca ttcgcgccag ctcggcaggt gcgatgaatt ttttccagtc gtgcgtgcct
     1261 ttggggacaa acttcaacag atattccgcc gccacaatca gatgcaggta cgatttcggg
     1321 tttttattga tggtagaaaa aaacaccatg ccgtccggtt tgaccagatt ggcacaggcg
     1381 cgcacgatgg cggcgggatc ggggacgtgt tccatcattt ccatgcacgt taccacatcg
     1441 aacgagtgcg gttccgcctc ggcaaggtct tccacgcgga tacattcgta ttcgatatcg
     1501 gcgacattgt tcaaagccgc gtgcaggcgg gcggtttcca acgactgctc cgccatgtcg
     1561 atgcccttta caaacgccgc gccgcgccgc gccatacttt ccgccaagat gccgccgccg
     1621 cagcccacgt ccaaaacccg tttgccgcac aaatccgcgt gtccgtcgat ataatccagc
     1681 cgcagcggat tgatgtcgtg caaggttttg aactcgcccg acttgtccca ccatttgtcg
     1741 gcaatccggc tgaatttggc gatttccccc tcatcgacat tatatttttt gtcggacatt
     1801 ttccctccca tctgacgaac cgcccactcc aaaacccaag attatactat ggaacacaat
     1861 gccgtctgaa agccttttcg ggcggcgcgg cgcaaatgcc ttacaaatcc ttacacttta
     1921 cggcataatg gcggctcgct ttttctggca gaaagacaaa atatgcccac caaaacccct
     1981 tcactgttcg gcggcgcgat gattatcgcc ggcacggtca tcggcgcagg tatgctcgcc
     2041 aacccgaccg ccacatccgg cgtatggttt accggctcgc tggccgtgtt gctgtacacc
     2101 tggttttcta tgctttccag cggcctgatg attttggaag tcaacaccca ttatccgcac
     2161 ggcgcaagtt tcgacacgat ggtcaaagac ctgctcggac gcggctggaa catcatcaac
     2221 ggtatcgccg tcgccttcgt tttatacctg ctcacctacg cctacatctt cgttggcggc
     2281 gacctgaccg ccaaaggctt aggcagcgcc ttgggcggca atgtttcact caccgtcgga
     2341 caactcgtct tcttcggcat cctcgccttt tgcgtatggg catccgcacg cttggtcgac
     2401 cgcttcacca gcgtcctcat cggcggcatg gtattaacct ttatttgggc aaccggcggc
     2461 ctgattgccg atgccaaaat gcccgtcctg ttcgacaccc aagcccccgt cggcaccaac
     2521 tactggattt acgccgccac cgccctgccc gtctgccttg cgtcattcgg tttccacggc
     2581 aacgtctcca gcctgctcaa atactttaaa ggcgacgcgc ccaaagtggc taaatccatc
     2641 tggacgggca cactgattgc gctggtgatt tacgtcctct ggcaaaccgc catccaaggc
     2701 aacctgccgc gcaacgcgtt cgcccccgtg attgccgccg aagggcaagt ctccgtcctc
     2761 atcgaaaccc tgtccaaatt cgcccaaacc ggcaatatgg ataaaatatt gtccctgttt
     2821 tcctatatgg cgatcgccac ctcgttttta ggcgtaacct taggtctgtt cgactacatc
     2881 gccgacatct tcaaatggaa cgacagcgtg tccggccgca ccaaaaccgc cgcgctgacc
     2941 ttcctgccgc ctctaatttc ctgcctgctc ttccccaccg gctttgttac cgccatcggc
     3001 tacgtcggcc tggcggcaac cgtctggaca ggcatcatcc ccgccatgct gctctaccgt
     3061 tcgcgcaaaa aattcggcgc aggcaaaacc tataaagttt acggcggctt gtggctgatg
     3121 gtttgggtct tccttttcgg catcgtcaac atcgccgcac aggtattgag ccaaatggaa
     3181 ctcgtccccg tatttaaagg ataaaggcaa aatgccgtct gaagcccgcc ggcggcttca
     3241 gacggcattg ccgcaacaaa cggcaaccgt attccggcac acagcgcatt accctgcccc
     3301 tcacgcacaa atcccgcccc gacaaaccgg gacgcaacca taaaggaaca atgatgaagc
     3361 tcaaaataga cattgcaacc aacaacttca aacacggcgg cggcacggaa cgctacacat
     3421 tggatttggt aaagggtctg aacagacaaa acatcacacc ggccgtttat gcgacgaaat
     3481 ttgatcacgg cattcctgaa tacgccatga tcgaacccca tcttgtcgat caacaccgga
     3541 cgctgaaaaa actacgctca ttcctctttt caagccggct cgctcaaacc agaaaaaaca
     3601 gtgccgccaa actgattgcc tgccaccacg ccgattacgc cgacctcctc atctgcggcg
     3661 gcacacactt gggctacctg caccatatgg cgcaaaaacc gaacctgctc gaccgtctcg
     3721 ccatacgccg caaccgcagc aactacgcca ccgccaaact gattatggcg cattcccata
     3781 tgatgcggcg cgaactggtc ggactgtacg gcgttccccc tgaaagaatc caagtcgccc
     3841 cccccccccc gcagatacgg aacgcttctt cccccaaccc ggagaaactg ccgacctgcg
     3901 cgccaaatac ggctttgccg accatgaaac cgttttcctg ttcccatcga ccggccacac
     3961 gcgcaaaggt ctggaactgc ttgccgactt tttcgaacat accggcctgc ccgtcaagct
     4021 tgccgttgtc ggctccccgc tcccccgccc tatgaaaaac gtcgtcggac tgggcttctg
     4081 caccgatatg cccgaactct accgcgccgc cgactttacc attatggcct ccctgtacga
     4141 acccttcggg ctggtcggtg tcgaatccgt cctatgcggc acacgcgtcg tcctctccga
     4201 aaacatggca tgtgcagagg tcatgaacga agaagccgga ttctttttct cacgccaaaa
     4261 cccggaaacc ctggcgcaag ccgttgccca agccgtcagc cttaaaaaac agggcggaca
     4321 ccgcctgtcc gacccgatgc gtgcactgaa ctacaacccg tccctgtcgc accacatcga
     4381 ccgactgacc gacatgctgg catccgtata acaaaatgcc gtctgaacga gttttcagac
     4441 ggcattgctg cgttatagtg gattaacaaa aatcaggaca aggcaacgaa gccgcagaca
     4501 gtacagatag tacggaaccg attcacttgg tgcttcagca ccttagagaa tcgttctctt
     4561 tgagctaagg cgaggcaata ccgtactggt ttttgttaat ccactatatc acgcggcaca
     4621 ggcgcatcac tgccgatatt tcagtataat gccacccccg acctgcccca acccaaagga
     4681 aacgcgatga aactcatcat tctcgaccgc gacggcgtca tcaatcagga ccgcgatgac
     4741 ttcgtcaaat ccgttgacga gtggatacct gtcgaaggca gcatggatgc ggtggcattt
     4801 ctgacgcagg caggctacac cgtcgccgtt gccaccaacc aatccggcat cgggcgcaaa
     4861 tattttaccg ttcaaaacct taccgaaatg cacgccaaaa tgcaccgcct cgtccgtcag
     4921 gcaggcggcg aaatcaacgg catctggttc tgcccgcaca ccgatgccga caactgcaac
     4981 tgccgcaagc ccaaaccggg tatgattgaa gacatcatcg gacgcttcaa cgcccaagcc
     5041 tcggaaacct ggctggtcgg cgacagcctg cgcgatttgc aggcaatcga tgccgtcggc
     5101 ggaaaacccg cgctggttct gaccggaaaa ggcaaaaaaa cgctctccca acacggacac
     5161 gaattacccg aacacacaca ggttttcgat accttgctcg atttctcaca atacatcatg
     5221 caggaaaacg ccgcaccgca agccgactga acataccgca ttccgacaag gcaaaaccat
     5281 gctcatcatc cgcaacctga tttactggct gatactctgt tccaccctga ttttcctctt
     5341 tccctttatg ctgctcgcct cgcctttccg agacggggcg cacaagatgg cgcgggtctg
     5401 ggtcaaaatc ctcaacctct cgctcaaaca catcgtcggg ctcaaatacc gcatcatcgg
     5461 cgcggaaaac atccccgacc gccccgccgt catctgcgcc aaacaccaaa gcggctggga
     5521 aacgctcgcc cttcaggaca tttttccgcc gcaggtttac gttgccaaac gcgagttgtt
     5581 caaaatcccc tttttcggct ggggcttgaa actggtcaaa accataggca tagaccgcaa
     5641 caaccgccgc gaagccaacg agcagctcat aaaacagggg ttggcgcgca aaaacgaagg
     5701 ctattggatt accattttcc ccgaaggcac acgccttgcg cccggaaaac gcggcaaata
     5761 caaactcggc ggcgcgcgca tggcgaaaat gtttgagatg gacatcgtcc ccgtcgccct
     5821 caacagcggc gaattttggc cgaaaaactc ctttctgaaa tatccggggg aaatcaccgt
     5881 cgtcatctgt ccgaccatcc cgcacgcaag cggcagcgaa gccgaattga tgggaaaatg
     5941 cgaacacctc atcgaaacgc agcagccgct catttccggc gcaggcccgt ttgccgccaa
     6001 aatgccgtct gaaaccgcat gaccgccttt gtccacaccc tttcagacgg catggaactg
     6061 accgtcgaaa tcaagcgccg tgccaagaaa aacctgatta tccgccccgc cggcacacat
     6121 accgtccgca tcagcgtccc accctgcttc tccgtctccg ctctaaaccg ctggctgtat
     6181 gaaaacgaag ccgtcctgcg gcaaacactg gcgaaaacac cgccgccgca aactgccgaa
     6241 aaccggctgc ccgaatccat cctcttccac ggcagacagc ttgccctcac cgcccatcaa
     6301 gacacgcaaa tcctgctgat gccgtctgaa atccgtgttc ccgaaggcgc acccgaaaaa
     6361 cagcttgcgc tgctgcggga ctttttggaa cggcaggcgc acagttacct gattccccgc
     6421 ctcgaacgcc acgcccgcac cacacaactg ttccccgccc cctcctcgct gacctctgcc
     6481 aaaaccttct ggggcgtgtg ccgcaaaacc acaggcatac gcttcaactg gcggctggtc
     6541 ggcgcaccgg aatacgttgc cgactatgtc tgcatacacg aactctgcca cctcgcccac
     6601 gccgaccaca gccgcgcgtt ctgggcactg acccgccgct tcgcacccta cacgcccgaa
     6661 gcgaaacagt ggctcaaaat ccacggcagg gaacttttcg ccttaggctg acgcggcccg
     6721 ggcaggcatc cgccaattca gacctcctcc caaacggaca tccggaaagc agcatcacag
     6781 ccggcgacac ataccgctgc cgccgctttc agacggcatc cgtgccggaa caggcacgcg
     6841 cccgcccgat tcaaaccgcg atgacgcttt gccgccggtt cggggcagga tggcggcaca
     6901 cacgccgtct gccgcgtttc atttcacacc actcttccga aacccgaaac ccgcccggtc
     6961 cgacgtgcgg tatgaaacgc ttaagctgac gcgaagtctt ttactgattt gcccgcgaaa
     7021 atgccgtctg aaaggttttc ggacggcatt tttttgcgtt tcccaggatg gcggcggatt
     7081 cgtaaaaggc ggtcagggtg gattgtagga tgggttgaga cctgccgaat ccgccgcatc
     7141 tgccaaatct accgccgtca ttcctacgaa agtgggaatc tagaacgcgg ggttaagaaa
     7201 acctgcatcc cgtcattccc acgaaagtgg ggatccagtt ttttgagttt cagtcatttc
     7261 cgataaattg ccttagcatt gaatgtctag attcccgcct gcgcgggaat gacgaatcca
     7321 tccatacgga aacctgcacc gcgtcatgcc tacgaacctg cattccgtca ttcccacgaa
     7381 agtgggaatc cagttttttg agtttcagtc atttccgata aattgcctta gcattgaatg
     7441 tctagattcc cgcctgcgcg ggaatgacga atccatccgt acggaaacct gcaccgcgtc
     7501 attcctacga acctacatcc cgtcattccc acgaaagtgg gaatccagga cgcaaaatct
     7561 caagaaaccg ttttacccga taagtttccg caccgacaga cctagattcc cgcctgcgcg
     7621 ggaatgacga atccatccat acggaaacct gcatcccgtc attcctacga acctacattc
     7681 cgtcattccc acgaaagtgg gaatccagaa tcccagactt tcagataatc tttgaatatt
     7741 gctgttgttc taaggtctag attcccgcct gcgcgggaat gacgggattt gaggtttctg
     7801 ttcgcgtcat tcccacgaac ctgcatcccg tcattcccac gaaagtggga atctagtttt
     7861 gtcggtgcgg aaacttatc
//
LOCUS       DAWFJR010000091         7747 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWFJR010000091 DAWFJR010000000
VERSION     DAWFJR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.091"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(799..2145)
                     /gene="mnmE"
                     /locus_tag="WGR04_001739"
     CDS             complement(799..2145)
                     /gene="mnmE"
                     /locus_tag="WGR04_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ0795251.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPPELKRIEIHSKSD
                     LHTSAAAAFETGADTVIPLSAKTGDGLDALKRTLLREAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELLAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            2276..2431
                     /locus_tag="WGR04_001740"
     CDS             2276..2431
                     /locus_tag="WGR04_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795252.1"
                     /translation="MEKRNTYALLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            2428..2859
                     /locus_tag="WGR04_001741"
     CDS             2428..2859
                     /locus_tag="WGR04_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ0795253.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2993..7330
                     /gene="app"
                     /locus_tag="WGR04_001742"
     CDS             2993..7330
                     /gene="app"
                     /locus_tag="WGR04_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ0795254.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGRNPDQHRFSYQIVKRNNYKPDNSHPYNGDYHM
                     PRLHKFVTDAEPVEMTSDMRGNTYSDKEKYPERVRIGSGHHYWRYDDDKHGDLSYSGA
                     WLIGGNTHMQGWGNNGVVSLSGDVRHANDYGPMPIAGAAGDSGSPMFIYDKTNNKWLL
                     NGVLQTGYPYSGRENGFQLIRKDWFYDDIYRGDTHTVFFEPRSNGHFSFTSNNNGTGT
                     VTETNEKVSNPKLKVQTVRLFDESLNETDKEPVYAAGGVNQYRPRLNNGENLSFIDYG
                     NGKLILSNNINQGAGGLYFEGDFTVSPENNETWQGAGVHISEDSTVTWKVNGVANDRL
                     SKIGKGTLHVQAKGENQGSISVGDGKVILDQQADENNKKQAFSEIGLVSGRGTVQLNA
                     DNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKESTVTITGNKDI
                     ATTGNNNSLDSKKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLSGGTNLNGNI
                     TQTNGKLFFSGRPTPHAYNHLNDHWSQKEGIPRGEIVWDNDWINRTFKAENFQIKGGQ
                     AVVSRNVAKVKGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEKTITDDKVIA
                     SLTNTDISGNVSLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNGDLSLVGNAQ
                     ATFNQATLNGNTSASGNASFNLSDHAVQNGSLTLSGNAKANVSHSALNGNVSLADKAV
                     FHFESSRFTGQISGSKDTALHLKDSEWTLPSGTELGNLNLDNATITLNSAYRHDAAGA
                     QTGSATDAPRRRSRRSLLSVTPPASVESRFNTLTVNGKLNGQGTFRFMSELFGYRSDK
                     LKLAESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLNFTLQNEHVDAGAWR
                     YQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALIAAGRDAVEKTES
                     VAEPARQAGGENVGIMQAEEEKKRVQADKDTALAKQREAETRPATTAFPRARRARRDL
                     PQPQPQPQPQPQRDLISRYTNSGLSEFSATLNSVFAVQDELDRVFAEDRRNAVWTSGI
                     RDTKHYRSQDFRAYSQQTNLRQIGMQKNLGSGRVGILFSHNRTENTFDDGIGNSARLA
                     HGAVFGQYGIGRFDIGISAGAGFSSGSLSDGIGSKIRRRVLHYGIQARYRAGFGGFGI
                     EPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHISITPYLSLS
                     YTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAKGPQLEAQHS
                     AGIKLGYRW"
BASE COUNT         2069 a         2202 c         1855 g         1621 t
ORIGIN      
        1 ggtttctgtt ttttccgata aattcctaaa actcaaaatt tcatcattcc cgcaaggaca
       61 gaaaaccaaa aacagaaacc taaaattcgt cattcccacg aaagtgggaa tccagaatcc
      121 cggactttta gataatcttt gaatattgct gttgttctaa ggtctagatt cccgcctgcg
      181 cgggaatgac gatatttctg tttttgattt tttgtttttg gggaatgacg ggatttgaga
      241 ttgcgggtat ttatcgggta aaacggaaat tatgcgttac gaaaatttat ccgaaatcac
      301 ggcaactttt ccaccgtcat tcccacgaaa gtgggaatcc aggtctgtcg gcacggaaac
      361 ttatcgagaa aaacggtttc tttatagtgg attaacaaaa atcaggacaa ggcgacgaag
      421 ccgcagacag tacaaatagt acggaaccga ctcactcggt gcttcagcac cttagagaat
      481 cgttctcttt gagctaagtc aaggcaacgc tgtactggtt tttgttaatc cactatagat
      541 tttacgtcct ggattcccgc ctgcgcggga atgacgatat ttcagttttc tgtttttgat
      601 ttttttgttt ttacaggaat aacggtcttt tcatatcgaa aaaagttgcc gtaccgcgcc
      661 gataatttcc gcctgcgcgg aatgaagatt taagcgttgc ccgaaattca aaaaaactaa
      721 aaccaaacgg atcggattcc cgtttttatg ggataacgga atgttcagcc ggacaccgcc
      781 ccggtgcttt cgatccgttt attttccgat acaaaacctc gaaaaaatca cgccgagcaa
      841 atcatctgcg gtaaattccc ccgtgatttc gccgcacgcg acctgagcca agcgcaaatg
      901 ttccgcaagc agctcgattt gatggttgcc gcacaatgcc gccagcgaca attcttcctg
      961 cgctgctttg agtgcgttga cgtgccgcgt ccgcgccaaa aacagccctt cgctttcgcc
     1021 ctgccaaccg gcctcgcgca acaacgtccg tttcagcgcg tccaagccgt cgccggtttt
     1081 cgccgacaac ggaatcaccg tatccgcacc cgtttcaaaa gccgccgccg cactcgtgtg
     1141 caaatcggat ttgctgtgga tttcgatgcg tttcaactcc ggcggcaacg cgtccaaaat
     1201 cgcccgtgtc ttttcattca aaccctcgcg cggatcgacc aacaccagcg cgacatcggc
     1261 ttcggatacg gctttgcggc tgcgttcgat gccgatgcgt tcgaccacgt cgtccgtctc
     1321 gcgcaaacct gccgtatcga caatatgcac cggcacgccg tcaatcagga tacgttccct
     1381 gaccgcgtcg cgcgtcgttc cggcaatatc ggtaacaatc gccacttcgt cgcccgccag
     1441 cgcgttcaac aggctggatt tgccgacatt cggcgcgccg accaatacga cattcagacc
     1501 ttcgcgcaaa atcgcgccct gctgcgcgtt ggcaagcaca tcatccacgg cgcggcgcaa
     1561 gccgtccagt ttgccgcgtg cgtctgccgc ttcgagaaaa tcaatgtctt cctcgggaaa
     1621 atctaacgtc gcttcgacca gcatccgcaa ggtaatcaag tcttcgacca gaccgtgtat
     1681 ccgccgcgaa aaatcgcctt tgagcgagcg caacgccaga cgcgccgccg aacggctgga
     1741 tgcgtcaatc aaatccgcca cgccttccgc ctgtgccaag tccagtttgt cgtttaaaaa
     1801 cgcacgcttc gtaaactcgc ccggttcggc aaggcgcgcg cccaattcca aacagcggtt
     1861 cagcagcata tccatcacta ccggcccgcc gtgtccctga agctcgataa catcttcgcc
     1921 cgtaaaactt gccggtgcgg caaaaaaaag caaaagcccg ctgtcgattg cctgaccgtc
     1981 cgcgtccgta aaatcagcat aggttgccac gcgcggcttg ggcgttttcc cacacaaagc
     2041 ctctgccatc ggcagcaggt ttttccccga tatgcgtatc acgcccacgc cgccgcgccc
     2101 gggtgcggta gcgactgccg caatcgttgg aacgttatcc gacataaaac ccccgaaaat
     2161 tcaaaacagc cgcgattata gcaaatgccg tctgaagtcc gacggtttgg ctttcagacg
     2221 gtataaaacc gcaaaaatgc ttgataaatc cgtccgcctg acctaatata accatatgga
     2281 aaaacgaaac acatacgccc tcctgctcgg tataggctcg ctgttgggcc tgttccatcc
     2341 cgcaaaaacc gccatccgcc ccaatcccgc cgacgatctc aaaaacatcg gcggcgattt
     2401 tcaacgcgcc atagagaaag cgcgaaaatg accgaaaacg cacaggacaa ggcgcggcag
     2461 gctgtcgaaa ccgtcgtcaa atccccggag cttgtcgagc aaatcctgtc cgacgagtac
     2521 gtgcaaataa tgatagcccg gcgtttccat tcgggaccgt tgccgccgcc gtccgacttg
     2581 gcgcaataca acgacattat cagcaacggg gcagaccgca ttatggcaat ggcggaaaaa
     2641 gaacaagccg tccggcacga aaccatacgg caagaccaaa ccttcaacag gcgcgggcaa
     2701 ctgtacggct tcatcagcgt catcctgata ctgctttttg ccgtcttcct cgtatggagc
     2761 ggctaccccg caaccgccgc ctcccttgcc ggcggcacag tggttgcctt ggcgggtgct
     2821 ttcgtgattg gaagaagccg agaccaaggc aaaaattaat gacaaatcct agggcgtgct
     2881 tcatatccgc ccgaacgccg aaccgcacat ataggcacat cccgcgcgcc gcccggaagc
     2941 ggaagccgcg ccctcccaaa caaacccgaa tcccgtcaga taaggaaaaa taatgaaaac
     3001 aaccgacaaa cggacaaccg aaacacaccg caaagccccg aaaaccggcc gcatccgctt
     3061 ctcgcctgct tacttagcca tatgcctgtc gttcggcatt cttccccaag cctgggcggg
     3121 acacacttat ttcggcatca actaccaata ctatcgcgac tttgccgaaa ataaaggcaa
     3181 gtttgcagtc ggggcgaaag atattgaggt ttacaacaaa aaaggggagt tggtcggcaa
     3241 atcaatgaca aaagccccga tgattgattt ttctgtggtg tcgcgtaacg gcgtggcggc
     3301 attggtgggc gatcaatata ttgtgagcgt ggcacataac ggcggctata acaacgttga
     3361 ttttggtgcg gaaggaagaa atcccgatca gcaccgtttt tcttaccaaa ttgtgaaaag
     3421 aaataattat aagcctgaca attcacaccc ttacaacggc gattaccata tgccgcgttt
     3481 gcataaattt gtcacagatg cagaacctgt cgaaatgacg agtgacatga gggggaatac
     3541 ctattccgat aaagaaaaat atcccgagcg tgtccgcatc ggctcaggac accactattg
     3601 gcgttatgat gatgacaaac acggcgattt atcctactcc ggcgcatggt taattggcgg
     3661 caatacacat atgcagggtt ggggaaataa tggcgtagtt agtttgagcg gcgatgtgcg
     3721 ccatgccaac gactatggcc ctatgccgat tgcaggtgcg gcaggcgaca gcggttcgcc
     3781 aatgtttatt tatgacaaaa caaacaataa atggctgctc aacggagttt tacaaaccgg
     3841 ctacccttat tccggcaggg aaaacggttt ccagctgata cgcaaagatt ggttctacga
     3901 tgacatttac agaggcgata cacataccgt cttttttgaa ccgcgcagta acggacattt
     3961 ttcctttaca tccaacaaca acggtacggg tacggtaaca gaaaccaacg aaaaggtttc
     4021 caatccaaag cttaaagtac agacagtccg actgtttgac gaatctttga atgaaactga
     4081 taaagaacca gtttacgcgg cagggggtgt taatcagtac cgtccaaggt taaacaacgg
     4141 tgaaaacctt tcttttatcg attacggcaa cggcaaactc atcttatcaa acaacatcaa
     4201 ccaaggcgcg ggcggtttgt attttgaagg tgattttacg gtctcgcctg aaaacaacga
     4261 aacgtggcaa ggcgcgggcg ttcatatcag tgaagacagt accgttactt ggaaagtcaa
     4321 tggcgtggca aacgaccgcc tgtccaaaat cggcaaaggc acgctgcacg ttcaagccaa
     4381 aggggaaaac caaggctcga tcagcgtggg cgacggtaaa gttattttag atcaacaagc
     4441 agatgaaaat aataaaaaac aagcctttag tgaaatcggc ttggtcagcg gcaggggtac
     4501 ggtgcaactg aatgccgata atcagttcaa ccccgacaaa ctctatttcg gctttcgcgg
     4561 cggacgtttg gatttgaacg ggcattcact ttcgttccac cgtattcaaa ataccgatga
     4621 aggggcgatg attgtcaacc acaatcaaga caaagaatcc accgttacca ttacaggcaa
     4681 taaagatatt gctacaaccg gcaataacaa cagcttggat agcaaaaaag aaattgccta
     4741 caacggttgg tttggcgaga aagatacgac caaaacgaac gggcggctca accttgttta
     4801 ccagcccgct gcagaagacc gcaccctgct gctttccggc ggaacaaatt taaacggcaa
     4861 catcacgcaa acaaacggca aactgttttt cagcggcaga ccaacaccgc acgcctacaa
     4921 tcatttaaac gaccattggt cgcaaaaaga gggcattcct cgcggggaaa tcgtgtggga
     4981 caacgactgg atcaaccgca catttaaagc ggaaaacttc caaattaaag gcggacaggc
     5041 ggtggtttcc cgcaatgttg ccaaagtgaa aggcgattgg catttgagca atcacgccca
     5101 agcagttttt ggtgtcgcac cgcatcaaag ccacacaatc tgtacacgtt cggactggac
     5161 gggtctgaca aattgtgtcg aaaaaaccat taccgacgat aaagtgattg cttcattgac
     5221 caataccgac atcagcggca atgtcagcct tgccgatcac gctcatttaa atctcacagg
     5281 gcttgccaca ctcaacggca atcttagtgc aaatggcgat acacgttata cagtcagcca
     5341 caacgccacc caaaacggcg accttagcct cgtgggtaat gcccaagcaa catttaatca
     5401 agccacatta aacggcaaca catcggcttc gggcaatgct tcatttaatc taagcgacca
     5461 cgccgtacaa aacggcagtc tgacgctttc cggcaacgct aaggcaaacg taagccattc
     5521 cgcactcaac ggtaatgtct ccctagccga taaggcagta ttccattttg aaagcagccg
     5581 ctttaccgga caaatcagcg gcagcaagga tacggcatta cacttaaaag acagcgaatg
     5641 gacgctgccg tcaggcacgg aattaggcaa tttaaacctt gacaacgcca ccattacact
     5701 caattccgcc tatcgccacg atgcggcagg ggcgcaaacc ggcagtgcga cagatgcgcc
     5761 gcgccgccgt tcgcgccgtt ccctattatc cgttacacct ccggcttcgg tagaatcccg
     5821 tttcaacacg ctgacggtaa acggcaaatt gaacggtcag ggaacattcc gctttatgtc
     5881 ggaactcttc ggctaccgca gcgacaaatt gaagctggcg gaaagttccg aaggcactta
     5941 caccttggcg gtcaacaata ccggcaacga acctgcaagc ctcgaacaat tgacggtagt
     6001 ggaaggaaaa gacaacaaac cgctgtccga aaaccttaat ttcaccctgc aaaacgaaca
     6061 cgtcgatgcc ggcgcgtggc gttaccaact catccgcaaa gacggcgagt tccgcctgca
     6121 taatccggtc aaagaacaag agctttccga caaactcggc aaggcagaag ccaaaaaaca
     6181 ggcggaaaaa gacaacgcgc aaagccttga cgcgctgatt gcggccgggc gcgatgccgt
     6241 cgaaaagaca gaaagcgttg ccgaaccggc ccggcaggca ggcggggaaa atgtcggcat
     6301 tatgcaggcg gaggaagaga aaaaacgggt gcaggcggat aaagacaccg ccttggcgaa
     6361 acagcgcgaa gcggaaaccc ggccggctac caccgccttc ccccgcgccc gccgcgcccg
     6421 ccgggatttg ccgcaacccc aaccgcaacc ccaaccccaa ccgcagcgcg acctgatcag
     6481 ccgttatacc aatagcggtt tgagtgaatt ttctgccacg ctcaacagcg ttttcgccgt
     6541 acaggacgaa ttggaccgcg tgtttgccga agaccgccgc aacgccgttt ggacaagcgg
     6601 catccgggac accaaacact accgttcgca agatttccgc gcctacagcc aacaaaccaa
     6661 cctgcgccaa atcggtatgc agaaaaacct cggcagcggg cgcgtcggca tcctgttttc
     6721 gcacaaccgg accgaaaaca ccttcgacga cggcatcggc aactcggcac ggcttgccca
     6781 cggcgccgtt ttcgggcaat acggcatcgg caggttcgac atcggcatca gcgcgggcgc
     6841 gggttttagc agcggcagcc tttcagacgg catcggaagc aaaatccgcc gccgcgtgct
     6901 gcattacggc attcaggcac gataccgcgc cggtttcggc ggattcggca tcgaaccgca
     6961 catcggcgca acgcgctatt tcgtccaaaa agcggattac cgctacgaaa acgtcaatat
     7021 cgccaccccc ggccttgcgt tcaaccgcta ccgcgcgggc attaaggcag attattcatt
     7081 caaaccggcg caacacattt ccatcacgcc ttatttgagc ctgtcctata ccgatgccgc
     7141 ttcgggcaaa gtccgaacgc gcgtcaatac cgccgtattg gctcaggatt tcggcaaaac
     7201 ccgcagtgcg gaatggggcg taaacgccga aatcaaaggt ttcacgctgt ccctccacgc
     7261 tgccgccgcc aaaggcccgc aactggaagc gcaacacagc gcgggcatca aattaggcta
     7321 ccgctggtaa caccggatat gccgaaaggg gtttgacgat gccaccgtgc gctgtcaaac
     7381 cctttttctg ccgccgcctt gtgcctgctc cattgtctga taaccgtcaa attgattttt
     7441 agcccatgtt tggtgggtcg gggaaatcta tatcttcgtc cgtgccgaaa tagtctgaga
     7501 cctttgcaaa attcctttcc ctcccgacag ccgaaacccc aacacaggtt ttcagctgtt
     7561 ttcagctgtt ttcgccccaa atacctccta attctaccca aataccccct taatcctcca
     7621 cggacacccg ataatcaggc atccgggctg ctttttaggc ggcagcgggc gcacttagcc
     7681 tgttggccgc tttcaaaagg ttcaaacaca tcgccttcag atggctttgt gcactcactt
     7741 tgctcag
//
LOCUS       DAWFJR010000092         7735 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWFJR010000092 DAWFJR010000000
VERSION     DAWFJR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7735
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.092"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            596..1471
                     /gene="prpB"
                     /locus_tag="WGR04_001743"
     CDS             596..1471
                     /gene="prpB"
                     /locus_tag="WGR04_001743"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ0795255.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVVGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIVRTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLKMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDTMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            1556..2710
                     /gene="prpC"
                     /locus_tag="WGR04_001744"
     CDS             1556..2710
                     /gene="prpC"
                     /locus_tag="WGR04_001744"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ0795256.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTELCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKAKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCIHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLHLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWAAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            3036..3980
                     /locus_tag="WGR04_001745"
     CDS             3036..3980
                     /locus_tag="WGR04_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HEZ0795257.1"
                     /translation="MYHKKFDKEILLKIKELYRYDNWHAVLAILYDYLIIIFSIFLSE
                     QSYWFLPLTILLIGSRQRALATILHEASHSALTKNKKFGQILGTYFSGYLIFQSWNSY
                     CVSHVKNHHPKLGTDSDPDYKYYKDSGIFQTYSRIEFFWRFCVSQILCLKLFRNFKYL
                     IKNRLFSDITRTELFKLLLSQLILFVIGTYFVGYKFYVIYWLLPYLTAFQMITWFIEL
                     SEHYPMIQTAKNNLEASRNRFSHAIEHFFTGIHGENYHLIHHLFPAIPFWNLEKAHKI
                     LMKDFDYQEVNKDFGGIFFSKNNNAPMWKVLWSKYEAA"
     gene            3967..4878
                     /locus_tag="WGR04_001746"
     CDS             3967..4878
                     /locus_tag="WGR04_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0795258.1"
                     /translation="MKLRDLLMGIFLAVSAALLNATIGIFSKILMEQGLSVQHIAFLK
                     TLTGFVFISILLCRTGFTRQIADISRKKEAILPLLLKVAICAFFGIYTLFFFETTAYQ
                     YGNAANVVVVLMASAAVSALILDSILLDERICISSVVGVGLAVLGIAMISWTGEGSLG
                     LILNAALAGSGYGCFSVLIKKFGLNGGIYLTRILMFFGSIFLFIPSLEGIEDIHWQWS
                     FIPPLLALSLLPTILGFYCTTKALDYLSAAKVQVTELAEPLFAAVLAWLFLNEIPEGR
                     FFVGAILIIAGIVSINGLYRPLLKRIE"
     gene            4969..7575
                     /gene="acnD"
                     /locus_tag="WGR04_001747"
     CDS             4969..7575
                     /gene="acnD"
                     /locus_tag="WGR04_001747"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ0795259.1"
                     /translation="MAANQRYRKPLPGTDLEYYNARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDSLGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELTGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAGKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRLGLQRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRIHPYAKQAFLASPPLVVAYALAGSIRFDIENDALGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILASSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVRNEDGSVRQGSLARVEPEGQTMRMWEAIETYMHRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPGTNRH
                     TLQLDGTETYDVVGERTPRGDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
BASE COUNT         2058 a         1768 c         1864 g         2045 t
ORIGIN      
        1 cttgtcctga tttaaattca atccactata tatttaaatt taattaaata atataaaaat
       61 caaatagata acaaactaca tcggattgct taaaaataat acattgtttt tatgtataaa
      121 atattttata agttttcagg attttgatta tcaaaaattt ttcttgattt cctgacaatt
      181 ttattgaaac aaataattaa aaattaatct agtttaatca tggaattaaa ataaaatatt
      241 aaaattatgt aatgagtctc cttaaaaatg tttgacattt tcagtcttgt gttttagatt
      301 agcgaaaaat aaaactacac aatactacaa aggaacatta ctatgaaacc aattcagatg
      361 ttttcccctt ttctgaataa tccccttgtt ttcttcttgt ctgcggtttt gccgcataat
      421 tccgaacggt ctgctgtttt tctttgattc gttttaaata tcaataagat aatttttccc
      481 atatattttt aatgattgga ttgggatgcc cgacgcgtcg gatggctgtg ttttgccgtc
      541 cgaatgtgat ggaagcctgt ccatactgaa aaaaagtcta taaaggagaa atatgatgag
      601 tcaacactct gccggagcac gtttccgcca agccgtgaaa gaatcgaatc cgcttgccgt
      661 cgtcggttgc gtcaatgctt actttgcacg attggccacc caaagtggtt tcaaagcaat
      721 ctatctgtct ggcggcggcg tggcagcctg ctcttgcggt atccctgatt tgggcattac
      781 cacaatggaa gacgtgctga tcgacgcacg acgcattacg gacaacgtgg atacgccttt
      841 gctggtggac atcgatgtgg gttggggcgg tgcattcaat attgtccgta ccattcgcaa
      901 ctttgaacgc gccggtgttg cagcggttca catcgaagat caggtagcgc aaaaacgctg
      961 cggccaccgt ccaaacaaag ccatcgtttc taaagatgaa atggtcgacc gtatcaaagc
     1021 tgccgtagat gcgcgtgttg atgagaactt tgtgattatg gcgcgtaccg atgcgctggc
     1081 ggtggaaggt ttagatgccg ctatcgaacg cgcccaagct tgtgtcgaag cgggtgcaga
     1141 catgattttc cctgaagcca tgaccgattt gaaaatgtac cgccaatttg cagatgcagt
     1201 gaaagtgccc gtgttggcga acattaccga gtttggttct actccgcttt atacccaaag
     1261 cgaactggct gaaaacggcg tgtcgctggt gctgtatccg ctgtcatcgt tccgcgcagc
     1321 aagcaaagcc gctctgaatg tttatgaagc gattatgcgc gatggcactc aggccgcagt
     1381 ggttgacact atgcaaaccc gtgccgagtt gtacgagcat ctgaactatc atgccttcga
     1441 gcaaaaactg gataaattgt ttcaaaaata agtaaccgct ttcagacggc ctttcaacaa
     1501 atccgcatcg accgtctgaa aaataaaacc cataaaaata caaaggagaa ataccatgac
     1561 tgaaactact caaaccccga ctttcaaacc taaaaaatcc gttgcgcttt ctggcgttgc
     1621 ggctggtaat accgaattgt gtaccgttgg ccgtaccggc aacgatttga gctatcgcgg
     1681 ttacgacatc ttggatttgg cacaaaaatg cgagtttgaa gaagtcgccc acctgctgat
     1741 tcacggtcat ttgcccaata aattcgagct ggccgcttat aaagccaagc tcaaatccat
     1801 gcgcggcctg cctatccgtg tgatcaaagt tttggaaagc ctgcctgcac atacccatcc
     1861 gatggacgtg atgcgtaccg gcgtatccat gctgggctgc attcatcctg aacgtgaaag
     1921 ccatccggaa agcgaagcac gtgacatcgc cgacaaactg atcgccagcc tcggcagcat
     1981 cctcttgtac tggtatcaat attcgcacaa cggcaaacgc attgaggttg aaagcgacga
     2041 agagaccatc ggcggtcatt tcctgcacct gttacacggc aaacgcccaa gcgaatcaca
     2101 catcaaagcc atgcacgttt cactgattct gtatgcagaa cacgagttca acgcttctac
     2161 ctttaccgcc cgcgtgattg ccggtacagg ctctgatatg tactccagca ttaccggtgc
     2221 aatcggcgcg ttgaaaggtc cgaaacacgg tggcgcgaac gaagtggctt acgatattca
     2281 aaaacgctac cgcaatgccg acgaagccga agccgacatc cgcgaacgca tcggccgcaa
     2341 agaaatcgtg atcggtttcg gtcatccggt gtacaccatt tccgaccctc gcaacgttgt
     2401 cattaaagaa gtggcacgtg gtttgagcaa agagaccggc gatatgcgcc tctttgacat
     2461 tgccgaacgt ttggagagcg tgatgtggga agagaaaaaa atgttcccga atctggactg
     2521 gttctctgcc gtttcctatc aaaaattggg cgtaccgacc gctatgttca caccgctgtt
     2581 cgtaatttcc cgtacaaccg gctgggcggc acacgttctc gagcaacgca aagacggcaa
     2641 aatcatccgt ccgagcgcga actacacagg ccctgaagat ttggcgtttg tggagattga
     2701 agaacgctga gacggaacac aaccgtagga gaccaccaat cggatttcag acgacgtgta
     2761 gcgcgggctt tgcctgcgga aaactgaaac ccgcctacgg ttccgctttt cagacggcct
     2821 gttgaaagat gcgagagaga aggttgtctg aaaaaccagc cgcaacgtct gccgtcattc
     2881 ccacccccgc ctaagcgagg acaggtcgcg gcgggaatgg cggaggcagc gcggcaactt
     2941 ttttcacggt tctgccaaaa tatgctatga aagaatgttc gtcagcataa tggagatgaa
     3001 gccgatatta taactaatac ggagaattgt gggatatgta ccataagaaa tttgataaag
     3061 aaattctttt aaaaattaaa gaattatata gatacgataa ttggcatgct gtacttgcta
     3121 tattatatga ttatttaata ataatttttt ctatattttt atctgagcag tcatattggt
     3181 ttctcccttt aacgatttta ttaataggaa gcaggcaaag ggcgttggca acaattttac
     3241 atgaagcctc tcattctgcc ttaactaaaa ataagaagtt tggacaaatt ttaggtacat
     3301 atttttctgg atatttaatt tttcaaagtt ggaatagtta ttgtgtttct catgtgaaga
     3361 atcatcatcc aaagttaggg actgattcgg atcctgatta taagtattat aaagactcag
     3421 gtatttttca gacatattca agaattgaat ttttctggag attttgtgtc tctcaaattt
     3481 tatgtttgaa attatttagg aattttaaat atttaattaa aaatagatta ttttcggata
     3541 ttacaagaac agagttgttt aagctacttc tttctcaatt aattctattt gttatcggta
     3601 catattttgt agggtataaa ttttatgtaa tttattggtt attaccatat ttaactgcat
     3661 tccagatgat aacttggttt attgagttgt ccgagcatta tccaatgata caaactgcca
     3721 aaaataattt agaagcgtca agaaataggt ttagtcatgc aatagagcat tttttcacgg
     3781 ggatacatgg agaaaattat catctaatac atcatttatt tccagccatt ccattttgga
     3841 atttggaaaa agcacataag atattaatga aagattttga ttatcaagag gtaaataaag
     3901 attttggagg gattttcttt tcgaaaaaca ataatgcacc tatgtggaaa gtattatgga
     3961 gtaaatatga agctgcgtga tttactgatg ggaatattct tggcagtttc tgcggccctt
     4021 ttgaatgcaa ccatcggcat attcagcaag atattgatgg agcagggctt gtctgttcag
     4081 catattgcat ttttgaaaac tttgacaggt ttcgtgttta tcagcatttt gctttgccgt
     4141 accggtttta ccagacagat tgcggatatt tcaagaaaga aagaggcaat tttgccgttg
     4201 ctgttaaaag tagcaatttg tgcttttttc ggaatttata cgttgttttt ctttgaaacc
     4261 acagcttatc aatatggcaa tgctgcgaat gtagtagttg tattaatggc atcggctgcc
     4321 gtatctgcct tgatattgga cagcatactg ttagatgaac gtatttgcat ttcttcagtc
     4381 gtcggtgtgg gtttggcagt attggggatc gcaatgattt cttggactgg agaaggaagt
     4441 ttagggttga ttctgaatgc cgcactggcg ggctcgggct acggttgttt ttccgttttg
     4501 attaagaaat tcggcctaaa cggcggtatt tatttgacac ggatattgat gttttttgga
     4561 agtatttttt tgtttatccc ttcattggaa ggtattgagg atatacattg gcaatggtct
     4621 tttattccgc cactcttggc attgtcttta ttgccgacga ttttaggatt ttattgtaca
     4681 actaaagcat tggattattt gagtgctgcg aaggtacagg taactgaatt ggccgagcca
     4741 ttgtttgctg ccgtactggc ttggttgttt ttgaatgaaa taccggaagg acgcttcttt
     4801 gtcggcgcca ttctgattat tgccggtatt gtgtctatca atgggctgta tcgaccattg
     4861 ttgaagcgaa ttgaataatt tttatttccc aaaatttcag acgacctctt caacaaacgt
     4921 cgtctgaaac cccaaaacca aaaaaataat agatttagga gaaccgacat ggctgccaac
     4981 caacgttacc gcaaaccgct gcccggtacg gatttggaat actacaacgc gcgtgcggcg
     5041 tgtgaggaca tcaagcccgg ctcttacgac aagctgcctt acacgagccg cattttggcg
     5101 gagaatttgg tcaaccgcgc ggacaaagtc gatttgccga cgctgcaaag ctggctgggt
     5161 cagctgattg agggaaaaca ggaaatcgac tttccttggt atccggcgcg ggtggtgtgc
     5221 cacgatattc tggggcagac cgcgttggtg gatttggcag gtctgcgcga tgcgattgcc
     5281 gaaaaaggcg gcgatcctgc caaagtgaat ccggtggtgc aaacccagct catcgtcgac
     5341 cactctctgg ccgttgaatg cggcggctac gatcctgatg ccttccgcaa aaaccgcgaa
     5401 atcgaagaca gacgcaacga agaccgtttc cacttcatca actggacaaa aaccgcattt
     5461 gaaaatgtgg acgtgattcc ggcgggcaac ggcattatgc accaaatcaa tctggaaaaa
     5521 atgtcgcccg tcgtccaagt caaaaacggc gtggcgttcc ccgatacctg cgtcggtact
     5581 gactcacata cgccgcacgt cgattcattg ggagtgattt ccgtgggcgt gggcggattg
     5641 gaagcggaaa ccgtaatgct gggacgcgcg tccatgatgc gcctgcccga tattgtcggt
     5701 gtggagctga ccggcaaacg gcaggcgggc attacggcga cggatattgt gttggcactg
     5761 accgagtttc tgcgcaaaga acgcgtggtc ggggcgtttg tcgaattctt cggcgagggc
     5821 gcgagaagcc tgtctatcgg cgaccgtgcg accatttcca acatgacgcc ggagttcggc
     5881 gcgactgccg cgatgttcgc tattgatgag caaaccattg attatttgaa actgaccgga
     5941 cgcgacgacg cgcaggtgaa attggtggaa acctacgcca aaaccgcagg cttatgggca
     6001 gatgccttga aaaccgccgt ttatccgcgc gttttgaaat ttgatttgag cagcgtaaca
     6061 cgcaacatgg caggcccaag taacccgcat gcccgttttg cgactgccga tttggccggc
     6121 aaaggcttgg caaaacctta tgaagagcct tcagacggac aaatgcccga cggtgcagtg
     6181 attattgccg cgattacttc gtgtaccaac acttccaatc cgcgcaacgt tgtcgctgcc
     6241 gcgctgttgg cacgcaatgc caaccgcctc ggcttgcaac gcaaaccttg ggtgaaatct
     6301 tcgtttgccc caggttcaaa agtagccgaa atctatttga aagaagcagg cctgctgcct
     6361 gaaatggaaa aactcggctt cggcatcgtc gccttcgcct gcaccacctg taacggcatg
     6421 agcggcgcgc tcgacccgaa aatccaaaaa gaaatcatcg accgcgattt gtacgccacc
     6481 gccgtattgt cgggcaaccg caacttcgac ggccgtatcc atccgtatgc gaaacaggct
     6541 ttcctcgctt cgcctccctt ggtcgttgcc tacgcgctgg caggtagcat ccgtttcgat
     6601 attgaaaacg acgcactcgg cgttgcagac ggcaaggaaa tccgcctgaa agacatttgg
     6661 cctgccgatg aagaaatcga tgccgtcgtt gccgaatatg tgaaaccgca gcagttccgc
     6721 gatgtgtatg taccgatgtt cgacaccggc acagcgcaaa aagcacctag tccgctgtac
     6781 gactggcgtc cgatgtccac ctatatccgc cgcccacctt actgggaagg cgcactggca
     6841 ggggaacgca cattaagagg tatgcgtccg ctggcgattt tgcccgacaa catcaccacc
     6901 gaccacctct caccgtccaa tgcaattttg gccagcagtg ccgcaggcga atatttggcg
     6961 aaaatgggtt tgcctgaaga agacttcaac tcttacgcaa cccaccgcgg cgaccacttg
     7021 accgcccaac gcgcaacctt cgctaatccg aaactgttta acgaaatggt gagaaacgaa
     7081 gatggcagcg tacgccaagg ttcgctggca cgcgtcgaac ccgaaggcca aaccatgcgt
     7141 atgtgggaag ccatcgaaac ctatatgcac cgcaaacagc cgctcatcat cattgccggt
     7201 gcggactacg gtcaaggctc aagccgcgac tgggctgcaa aaggcgtacg cctcgccggc
     7261 gtggaagcga ttgttgccga aggcttcgag cgcatccacc gtaccaactt gatcggtatg
     7321 ggcgtgttgc cgctgcaatt caaaccgggt accaaccgcc acaccctgca gctggacggt
     7381 acggaaacct acgacgttgt cggcgaacgc acaccgcgcg gcgacctgac cctcgtgatt
     7441 caccgtaaaa acggcgaaac cgtcgaagtt ccggttacct gccgcctcga taccgcagaa
     7501 gaagtattgg tatatgaagc cggcggcgtg ttgcaacggt ttgcacagga ttttttggaa
     7561 gggaacgcgg cttagaggtc gtctgaaaag caagacgtag cgtgggtcgg gttcaacatt
     7621 ttgctcattc acgtaattct cgatatggca ggcatctact gtaaatcgtc attcccgcgc
     7681 aggcgggaat ccagaaagtg gaattgagga aaccttttta tccgatgagt ttctg
//
LOCUS       DAWFJR010000093         7593 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWFJR010000093 DAWFJR010000000
VERSION     DAWFJR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7593
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.093"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(81..359)
                     /locus_tag="WGR04_001748"
     CDS             complement(81..359)
                     /locus_tag="WGR04_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795260.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
     gene            complement(366..650)
                     /locus_tag="WGR04_001749"
     CDS             complement(366..650)
                     /locus_tag="WGR04_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795261.1"
                     /translation="MFGGRLKNAPSITAKHFLKENIMKFINTCRKYGAKLAVVTAAPL
                     ALAAHANATLPDTAKNALEAAKADGMEAGWIVVGVFAALFVFSIVKRVMK"
     gene            complement(658..855)
                     /locus_tag="WGR04_001750"
     CDS             complement(658..855)
                     /locus_tag="WGR04_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795262.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            complement(860..1150)
                     /locus_tag="WGR04_001751"
     CDS             complement(860..1150)
                     /locus_tag="WGR04_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795263.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            complement(1195..2538)
                     /locus_tag="WGR04_001752"
     CDS             complement(1195..2538)
                     /locus_tag="WGR04_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ0795264.1"
                     /translation="MEDKQGMTKAVVGVMTDAPADGRKPATASNLPPLSNRGGTETET
                     AGRVQEVFECYETYITDGKGNLLGVPLRRGVSDSAFIDQISFSFHEKTFFDKYGVRVS
                     LLEDEDFIRAASMLAEEVFGFGLYKESKGSGGRFYERCWLMGSEDVLYGRVHFGGQNN
                     TILFELTGTGCGVAKEGWESRLFAFLTNAIRPKITRVDIAKDFFNGEYSPNQAREDRN
                     KGMFTCHHVKPKGECLGSDWEEDDEAKMTKGKTYGIGSRESSKYVRVYEKGKQLGDKT
                     STWTRFEIEFKAKDIVIPFEVLQNPGEYFGGAYPICERFVQKATRIHAVKEDKVISAD
                     RYLEWVKKQFGRAANGLKFIFPELDKAKLFELIEPSHHKLPKSLAPEAYDCAFLKAQA
                     IHEQPAFKPYKDPYYMYEYYENLEKQLEQQKHVNNEESYNNFIYDKFARLPISWA"
     gene            complement(2610..2855)
                     /locus_tag="WGR04_001753"
     CDS             complement(2610..2855)
                     /locus_tag="WGR04_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795265.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILRPLYIASLRRDD"
     gene            complement(3143..3485)
                     /locus_tag="WGR04_001754"
                     /pseudo
     CDS             complement(3143..3485)
                     /locus_tag="WGR04_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3721..5148
                     /locus_tag="WGR04_001755"
     CDS             3721..5148
                     /locus_tag="WGR04_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ0795266.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLYDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQSRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            5217..6620
                     /locus_tag="WGR04_001756"
     CDS             5217..6620
                     /locus_tag="WGR04_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ0795267.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTGLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(6784..>7593)
                     /locus_tag="WGR04_001757"
     CDS             complement(join(6784..7316,7316..>7593))
                     /locus_tag="WGR04_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEZ0795268.1"
                     /translation="YYEQCKNISQTAATFNLSRNTLYLWIRLKKQTGSLKHQVTGLNA
                     VKSDRQKPAQYIGQHQDAYLHEIAKHFDCTAATVCYALKQMGITRKKRPTTYKEQDPA
                     KVTHYLTQPAEFSDYQRVYLDETGFDRYLFRPYARSLKGQIVKAQISGKRYQRLSLVS
                     AQVGNRLIAPMVYQNTMTGVFFEAWFQQCLLPALTQKSVIILDNARFHRMGVLREMAE
                     KLGHKVLPLAPYSPELNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
BASE COUNT         1893 a         1810 c         1897 g         1993 t
ORIGIN      
        1 aacttcatgc gctcaattct taaacagaac cgccccgatt aatacgggta cggaaacgcc
       61 gagataaaaa taaaaatcca tcatttcaaa acctttttca gcagggaaac aaagtaaacg
      121 gacgcgagga tgccgaatac tatccagcct gtttcaagac cgctttgcag gttgtctttc
      181 ggactgcatt ccgccaataa aagccttagc ggctgaccgt ccgacatctt ccacaggctg
      241 ccgttatatt ccggcctgac aatctgtccg ttttctttga ttcttggtac taccaagctg
      301 aaataaaggt tttcagcctg gtgcttctca agacatttat ttccgacttg gtagtacatg
      361 ccgtcttact tcatcactct cttaacgatg gaaaagacaa aaagcgcggc gaaaacgccc
      421 actacaatcc aaccggcttc cataccgtcc gcttttgcgg cttccaaagc gttttttgcc
      481 gtatcgggca acgttgcatt tgcatgtgcg gccaaagcca ggggagcagc tgttacaaca
      541 gccagttttg cgccgtattt acggcaggtg ttaataaatt tcatgatatt ttccttcaaa
      601 aagtgtttgg cggtaatgga tggagcgttt ttcagacgac cgccgaacat ccgaaaatca
      661 gtctttcaaa aatccgaata cgacaaattc gtattggttg ccgatttctt ccaaacctgc
      721 gttaatcgct tcttcgaagt cgtagaaata atcggcattg gtgattaatt tggtatgtcc
      781 gatgtcgccc gtttcaggag agagatacag aaagtcccct gttgatacgg actggacaac
      841 atagactttc tgcattcaat cagcctttct tcacgagttg aaaaccgatg actttcagtt
      901 tttgggtttt gcccgtagtg acgatttcta cgttcaggtt tgcttcgatc ggaaattggg
      961 cgtttcggaa ctgctcgaaa ttggcagagc cgccgaaatc gtattcagta gtagagctgc
     1021 ccaatgcgtt gccttgggag ctgtctaagg gtgtggcgac aatcaggcgg caatagtcga
     1081 agctcttgcc ttcgatttgt ccgttgaatt ttttaacgcc gacgatgtga ccttgaagtt
     1141 ggatgttcat tttttggttt ccttgtgtga ttaaacgtct ttcgggcaga cactttaagc
     1201 ccatgaaatc ggtagtcttg cgaatttgtc gtaaatgaag ttgttatagc tttcttcatt
     1261 gttgacgtgt ttttgctgtt caagctgttt ttcaagattc tcgtaatatt cgtacatata
     1321 gtaagggtct ttgtacggtt tgaatgcggg ctgttcatga atggcttgag ctttcaaaaa
     1381 ggcgcagtcg taggcttcgg gagccaaaga cttgggcagc ttgtgatgac tcggctcaat
     1441 cagttcaaac agtttggctt tgtccaattc gggaaaaatg aatttcagac cgtttgccgc
     1501 acgtccgaac tgttttttta cccattcaag gtagcggtcg gctgaaatga ccttatcttc
     1561 cttaaccgcg tgtatgcgcg ttgccttttg gacgaatcgt tcgcaaatcg gatatgcgcc
     1621 gccgaaatat tcgcccggat tctgcaaaac ttcgaaaggg ataacgatgt cttttgcttt
     1681 gaattcaatt tcaaagcgcg tccatgtgct tgttttatcg cccaactgct tgcctttttc
     1741 atagacgcgg acatatttgg acgattcacg ggagccgata ccataggtct tgcctttggt
     1801 cattttggct tcatcgtctt cttcccaatc tgaccccaaa cattcgcctt ttggtttgac
     1861 gtgatgacag gtaaacatac ctttatttcg gtcttcacgg gcttggttcg ggctgtattc
     1921 gccgttgaaa aagtcttttg cgatgtcaac gcgtgtgatt tttgggcgga ttgcattagt
     1981 cagaaatgcg aaaagtcgtg attcccagcc ttcttttgcg acgccgcaac cggtgccggt
     2041 cagttcgaaa aggatggtat tgttttgtcc gccaaaatgg acgcgaccgt ataggacgtc
     2101 ttccgaaccc atcaaccaac agcgctcata aaaacgaccg cccgagcctt tggactcttt
     2161 gtagagaccg aaaccgaaaa cttcttcggc gagcatggac gcggcgcgaa taaaatcttc
     2221 gtcttccaaa agacttacac gaacgccgta tttatcgaaa aaggtttttt catgaaatga
     2281 aaagctaatt tgatcaatga aagccgaatc tgatacaccg cgccgaagag gaacgcctaa
     2341 caggtttcct ttaccgtccg ttatgtacgt ttcgtaacat tcgaagactt cctgaaccct
     2401 gcctgccgtt tcggtttctg taccccccct gttagataag gggggaaggt ttgaagcggt
     2461 tgccggcttc ctgccgtccg ccggtgcgtc cgtcatcacg ccgacaaccg cctttgtcat
     2521 cccttgctta tcttccatgg tgcgaatcct caaaaacggg caaaaaaaaa gccctgttac
     2581 ttgtagaaag taaaggacgt taatttttgt taatcgtccc ttcttaggga cgcaatatat
     2641 aagggacgca atatataagg ttttaaaacg ctaagaacaa aatccgataa cggctctgaa
     2701 gaagtaagtc tttctttatt gcacaagata gcaaacttcc acggcattct cgccccaccc
     2761 gtgccgtcca agcctttttc aaagacatag atggttttct tggcaatcac ttcgtcagtt
     2821 tggcggtcga taatccgtat ggtcgcacct gccacccagt gcctgcgcag cttggagtcg
     2881 acattgtttt cgaacgttac cgcataacgg gcggggtgta tatgattaag tgtttgattt
     2941 atcggaaaat ctttacctga ataccgaata atatcggaat ggttgggttg caggacatcc
     3001 acatagcggt attccccatc gctaaaaaat cctaggaaac gagcaataaa atttacgcct
     3061 tcgctggtct gtaaagccgc attgtcccac atcgggtctc tggtttttgc atctgccgaa
     3121 acggtacgct caggtactac cttcaacagc ataatccctt ccacattgtc cgccgtctgg
     3181 taaatctttc ccccgccgtt ttgcattgtt cgttaaacac ggcttcggct tctttgtatt
     3241 ttctgtccca ctcttcttgt gcctgtattt cttctttgct cgggccgaat tgtttgggaa
     3301 taatccaaac aaacagcatc aggatagcgg cggcggtcag gctgcctgaa aggattttgc
     3361 cgaggttttt tgtaggcaaa gcggacgaga aaccaaagca acagcagcat ggtgtcccaa
     3421 tagccgattg cgaataggat gaccaaacct tctaggaaat ggcgtaaatc gtttgtggta
     3481 aacatgggta gttcctgtgg ttaaatgtgc aggctgcttt ttgccgaacc ttgccgcatc
     3541 tcaaaagcag cctgcgcttc agcgttgcgt tacgcagtaa aataatgaat atttgtaacg
     3601 acttgggtat tttttgtcaa tattcccgcc tttcccttaa cagctgccgc gctttccgtt
     3661 aaaattcatt tacatattta tattgtttcc tgtttctata ttgccaaggt tatacccgtt
     3721 atgtttttct ccgccctgaa atcctttctt tctcgctaca ttactgtatg gcgcaatgtt
     3781 tgggcggtgc gcgaccagtt ggaaccgccc aaacgcacgg cggaagaaca ggcgtttttg
     3841 cccgcgcatt tggaactgac cgatacgccg gtctctgccg ctccgaaatg ggcggcgcgt
     3901 tttattatgg cgtttgcgct tttggctttg ttgtggtcct ggttcggcaa aatcgatatt
     3961 gtggcggcgg cttcgggcaa aacggtgtcg ggcgggcgca gcaaaaccat ccagccgctg
     4021 gaaacggcgg tggttaaggc ggtacatgtg cgcgacgggc agcatgtgaa acagggagaa
     4081 acgctggcgg aactggaggc tgtgggaaca gacagcgatg tggtgcagtc ggagcaggct
     4141 ttgcaggctg cccaattgtc caaactgcgt tatgaagcag tattggcggc attggaaagc
     4201 cgtaccgtgc cgcatatcga tatggcgcaa gcacggtctt taggtctcta cgatgccgat
     4261 gtgcaatcgg cgcaggtgtt ggcgcagcac cagtatcagg catgggcggc gcaggatgcg
     4321 caattgcagt cggctttgcg cggccatcag gcggaattgc agtcggccaa ggcgcaggag
     4381 cagaagctgg tttcggtggg ggcgatcgag cagcagaaaa cagcagacta ccgccgtttg
     4441 cgggccgaca attttatttc ggaacatgcg tttttggagc agcagagcaa atcggtcagc
     4501 aattggaacg atttggaaag tacgcgcggt cagatgaggc agattcaggc ggccattgca
     4561 caggcggagc agagtcgggt gctgaatacg cagaacctga aacgcgatac gctggatgcg
     4621 ctgcgccagg caaacgaaca gattgaccaa taccgcggcc aaacggataa ggcaaagcag
     4681 cggcagcagc tgatgacaat acagtcgcct gcggacggca cggtgcagga attggccacc
     4741 tatacggtgg gcggtgtggt gcaggctgcc caaaaaatga tggtggttgc gcccgatgac
     4801 gacaaaatgg acgtggaagt tttggtattg aacaaagaca tcggttttgt ggaacaggga
     4861 caggatgcgg tggtgaagat tgagagtttt ccctatacgc gctacggtta tctgacgggc
     4921 aaggtgaaaa gtgtcagcca tgatgcggta agccacgaac agttgggctt ggtttatacg
     4981 gcggtggtgt cgctggacaa acataccttg aatattgacg gcaaagcagt gaatctgacg
     5041 gcgggcatga atgtcacggc ggagattaaa acgggtaaac ggcgggtgct ggattatctg
     5101 ttaagcccgc tgcaaaccaa attggacgaa agctttaggg agcgataggc ggatccatac
     5161 tgggcatttg ttatccgccg gttcggacat gcagactgcc tgaaaccatt gcccggatga
     5221 cattgctcaa tctaatgata atgcaagatt acggtatttc cgtttgcctg acactgacgc
     5281 cctatttgca acatgaacta ttttcggcta tgaaatccta tttttccaaa tatatcctac
     5341 ccgtttcact ttttaccttg ccactatccc tttccccatc cgtttcggct tttacgctgc
     5401 ctgaagcatg gcgggcggcg cagcaacatt cggctgattt tcaagcgtcc cattaccagc
     5461 gtgatgcagt gcgcgcacgg caacaacaag ccaaggccgc attccttccc catgtatccg
     5521 ccaatgccag ctaccagcgc cagccgccat cgatttcttc cacccgcgaa acacagggat
     5581 ggagcgtgca ggtgggacaa accttatttg acgctgccaa atttgcacaa taccgccaaa
     5641 gcaggttcga tacgcaggct gcagaacagc gtttcgatgc ggcacgcgaa gaattgctgt
     5701 tgaaagttgc cgaaagttat ttcaacgttt tactcagccg agacaccgtt gccgcccatg
     5761 cggcggaaaa agaggcttat gcccagcagg taaggcaggc gcaggcttta ttcaataaag
     5821 gtgctgccac cgcgctggat attcacgaag ccaaagccgg ttacgacaat gccctggccc
     5881 aagaaatcgc cgtattggct gagaaacaaa cctatgaaaa ccagttgaac gactacaccg
     5941 gcctggacag caaacaaatc gaggccatag ataccgccaa cctgttggca cgctatctgc
     6001 ccaagctgga acgttacagt ctggatgaat ggcagcgcat tgccttatcc aacaatcatg
     6061 aataccggat gcagcagctt gccctgcaaa gcagcggaca ggcgcttcgg gcagcacaga
     6121 acagccgcta tcccaccgtt tctgcccatg tcggctatca gaataacctc tacacttcat
     6181 ctgcgcagaa taatgactac cactatcggg gcaaagggat gagcgtcggc gtacagttga
     6241 atttgccgct ttataccggc ggagaattgt cgggcaaaat ccatgaagcc gaagcgcaat
     6301 acggggccgc cgaagcacag ctgaccgcaa ccgagcggca catcaaactc gccgtacgcc
     6361 aggcttatac cgaaagcggt gcggcgcgtt accaaatcat ggcgcaagaa cgggttttgg
     6421 aaagcagccg tttgaaactg aaatcgaccg aaaccggcca acaatacggc atccgcaacc
     6481 ggctggaagt aatacgggcg cggcaggaag tcgcccaagc agaacagaaa ctggctcaag
     6541 cacggtataa attcatgctg gcttatttgc gcttggtgaa agagagcggg ttagggttgg
     6601 aaacggtatt tgcggaataa agcaggctga aacggttatg aaattcccaa agcagcctgc
     6661 accccgtttc gaaagtgcag gctgctttgg gattgatccg atactttcac attctcattg
     6721 cctttaaata aggcacagtt aaaaacacat ttaaattaag tgcgaattaa ttcaaatgta
     6781 tattcaatta aaatcaaaat aggacagtag tgcatcgtca aatcgggcgt aatcagacaa
     6841 aacggttcgc agataccgct taatattcgc ccacactttc tcaatcgggt tgagctcagg
     6901 tgaataaggt gcaagaggca ataccttatg tcccaatttt tccgccattt cccgtaagac
     6961 acccatacgg tgaaatcgcg cattatctaa aataatcacc gatttttgag tcaatgcggg
     7021 cagtaggcat tgctgaaacc acgcttcaaa aaagactccg gtcatcgtat tttgataaac
     7081 catcggagca atcagccggt tgccgacttg tgcggacacc aaagataagc gttggtatct
     7141 ttttccactt atctgcgctt tcactatttg ccctttcagg ctgcgggcat agggacggaa
     7201 caggtagcgg tcaaatcctg tttcatccaa ataaacacgt tggtagtcgg aaaattcggc
     7261 cggctgtgtc aaataatgcg ttactttggc cgggtcttgt tctttgtaag tggtggtctt
     7321 tttttgcgcg ttatccccat ctgtttgagt gcatagcaaa cggtggctgc cgtacaatca
     7381 aaatgtttgg cgatttcatg cagataggca tcctggtgtt gcccaatata ttgagccggt
     7441 ttttgcctat ccgatttgac ggcatttaga ccggtaactt gatgttttag gctgcctgtt
     7501 tgttttttaa ggcgaatcca caggtaaagc gtgtttcttg acaagttaaa cgttgctgcg
     7561 gtttggctga tgtttttgca ttgttcgtaa tat
//
LOCUS       DAWFJR010000094         7583 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWFJR010000094 DAWFJR010000000
VERSION     DAWFJR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7583
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.094"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            141..722
                     /locus_tag="WGR04_001758"
     CDS             141..722
                     /locus_tag="WGR04_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0795269.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(784..1638)
                     /gene="folD"
                     /locus_tag="WGR04_001759"
     CDS             complement(784..1638)
                     /gene="folD"
                     /locus_tag="WGR04_001759"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ0795270.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            1861..1938
                     /locus_tag="WGR04_001760"
     tRNA            1861..1938
                     /locus_tag="WGR04_001760"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1896..1898,aa:Pro,seq:tgg)
     gene            1963..2039
                     /locus_tag="WGR04_001761"
     tRNA            1963..2039
                     /locus_tag="WGR04_001761"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1997..1999,aa:Arg,seq:tct)
     gene            2067..2142
                     /locus_tag="WGR04_001762"
     tRNA            2067..2142
                     /locus_tag="WGR04_001762"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2100..2102,aa:His,seq:gtg)
     gene            2354..2860
                     /locus_tag="WGR04_001763"
     CDS             2354..2860
                     /locus_tag="WGR04_001763"
                     /inference="COORDINATES: protein motif:HMM:NF013621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HEZ0795271.1"
                     /translation="MVDAWSVPDFESKICPPESLAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPVNPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            2857..4635
                     /locus_tag="WGR04_001764"
     CDS             2857..4635
                     /locus_tag="WGR04_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ0795272.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            4748..5572
                     /locus_tag="WGR04_001765"
     CDS             4748..5572
                     /locus_tag="WGR04_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ0795273.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAQAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADAEEIRKITSLYGKLNLKSCK"
     gene            complement(6335..7123)
                     /locus_tag="WGR04_001766"
     CDS             complement(6335..7123)
                     /locus_tag="WGR04_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ0795274.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRIAQPAMITVSLRR
                     TGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDVGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(7337..7531)
                     /gene="thiS"
                     /locus_tag="WGR04_001767"
     CDS             complement(7337..7531)
                     /gene="thiS"
                     /locus_tag="WGR04_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ0795275.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQKPFAVAVNTVFIPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT         1620 a         1949 c         2265 g         1749 t
ORIGIN      
        1 tatgtatggc gtaaaggaag cgttttaacg ccgtattggg aaaatgtaaa gttctttatc
       61 gggatttctt taagtattgg cgttttcggt gtcgtggcgg tagaatcggg gtcagttaag
      121 aacaattatc aggaacggat atggaagaaa aaaacgacta caccgacgcg gcatccgaca
      181 accgcaacgg gcaggaaatt gaagtcggaa ttgccggcgc aggcgaccgg atacttgccg
      241 cgctgctgaa ccaattgttt acctttttgg ttttgttggt gccgtttgcc ggactgattg
      301 cttttgccat caagaatgaa ggcaggattg gcggcagtga ggaaatattc gggctgcttt
      361 tgggcatgac ttccttttgg gtaggactgg cgggcatttt ggtttatacc gtcgttcaaa
      421 tctactatat gagtcgggac gggcagtcat tgggtaagaa aatcatgaga atccgcgtgt
      481 tgaaaaccga cggccgcaat cccggttttg tcggcacggt tttggtacgc gaaatcgcat
      541 ggtcggtttt ggttgccatt attgccgccg ttatcggtct tgcagtaggt gacaacggag
      601 aaaacgccat caacctgctg gcattccttg ccaactttgt cctgctcttt atggtcaaac
      661 gcgaccgccg cacgctttac gacatactgg cggatacggt tgtcgtcaag ctgcccaaat
      721 aaacggacac ggcaaaatgc cgtctgaaag cctttcagac ggcattttta tcttcagaac
      781 cgctcaagca tcgtgcagtg aagccgcgtg cagggtgttt tccatcaatg tggcaatcgt
      841 catcggaccc acgccgccgg gaacgggcgt aatcatcgcc gcccgttctt ttgccgtttc
      901 aaattccacg tcgccgcaca ggctgccatc gtccaaacgg ttgatgccca catcaataac
      961 gaccgcgcca ggtttgatcc attcgccttt gacaaagttc ggaatgccta cgccgaccac
     1021 caaaatatcg gctccggcaa cctcgtctgt cagattttcg gttgcgctgt ggcagaccgt
     1081 taccgttgcg cgcgccagca gcagttccaa agcctgcggg cggccgacga tattcgacgc
     1141 gccgaccacg accgcttttt tccccttcgg atcaatgccg taagcttcca aaagcgtcat
     1201 cacgcccttg ggcgtacacg ggcgcatcag cggcattttg accgccagcc tgccgacatt
     1261 gtaaggatgg aagccgtcca cgtccttatc cggcgaaata cgttccaaaa ccgcctggct
     1321 gtcgaggtgc ttcggcagcg gtagctgaac cagaataccg tccacttcgg aatcggcatt
     1381 caggcggtcg accagtgcca gcagttcttc ctgcgatgtt gattcgggca gctcgtaaga
     1441 cagtgatttg atgccgcatt tttggcaggc agttttcttg ttgcggacat aaaccgcgct
     1501 ggcagggtcg cctccgacca aaaccacggc caggcaaggc atgtgcagat tgtcctgttg
     1561 gcgttgcgcc accgcttcgg caaccgcctg caggcgtttt tgcgaaactt ctttaccatt
     1621 gatcagttgt gccgacatat ccgtcctttt gttaaacagg tagaaaatgc tgaaaattat
     1681 cgcattttcc gtttgttttt acagcaggat gccgaaaata aatttgccct gaaataaaat
     1741 acggctgttt ggacggatta aatatttttt caaatcaaaa aactatattt ttcatcttgg
     1801 tgtcttgaaa ataaatattg acgcatcttt ccctgagttg tatagttcgc ttcttcaagt
     1861 cggggcgtag cgcagcccgg ttagcgcatc tgctttggga gcagagggtc gtgagttcga
     1921 atcccaccgc cccgaccaat caatcagcac gaatcggcat gagcgcccgt agctcaaccg
     1981 gatagagcac cgaccttcta agtcgggggt tacaggttcg attcctgtcg ggcgtgccaa
     2041 gaatttgatt gaaaaccgtt tttatggtgg ctgtagctca gttggtagag ccccggattg
     2101 tgattccggt tgtcgtgggt tcgagcccca tcagccaccc cagataaagg cccgtacttt
     2161 tcggtacggg tctttttccg tttgcacgta ctgtccgcac cgtcgtaccg gtgcggcggt
     2221 atgccgtctg aaagccgggc cggtcatacg gaaaccgctt cagacggcat ttgcgccgcc
     2281 ggctttgcct ttcgcatatg cttctgtatc atgtgggttt ttctgtcttt tatggttttt
     2341 gtcggaggct ttgatggttg acgcttggtc tgtccctgat tttgaatcga aaatctgtcc
     2401 gcccgagtcg ttggcggcgc gtttggcgtt gttgccgcgc ccgctggtgt ttaccaacgg
     2461 ctgtttcgac atcctccaca gggggcatgt tacttatctg gcgcaggcgc gttcgatggg
     2521 gggcgcgttg gtgctggcgt tgaataccga tgcttcggtg cggcgtctgg gcaagggcgg
     2581 tgaccgcccg gttaatcctt tggagaaccg tgccgccgtt gccgccgctt tggaaagtgt
     2641 ggatttggta acgtggtttg acggggatac gccggcggcg ttgattgagg cggtcaaacc
     2701 tgagattttg gtcaagggcg gcgattgggc tgcggataag attgtcggtg cggcagaaac
     2761 gttggcgcgc ggcggtcagg tgttttcaat tccgtttctg caccagactt cgacaacgaa
     2821 gactttggca aaaatccgtg cggcagaggg cggaaaatga cggttttgaa gccttcgcac
     2881 tggcgggtgt tggcggagct tgccgacggt ttgccgcaac acgtctcgca actggcgcgt
     2941 atggcggata tgaagccgca gcagctcaac ggtttttggc agcagatgcc ggcgcacata
     3001 cgcgggctgt tgcgccaaca cgacggctat tggcggctgg tgcgcccatt ggcggttttc
     3061 gatgccgaag gtttgcgcga gctgggggaa aggtcgggtt ttcagacggc attgaagcac
     3121 gagtgcgcgt ccagcaacga cgagatactg gaattggcgc ggattgcgcc ggacaaggcg
     3181 cacaaaacca tatgtgtgac ccacctgcaa agtaagggca gggggcggca ggggcggaag
     3241 tggtcgcacc gtttgggcga gtgtctgatg ttcagttttg gctgggtgtt tgaccggccg
     3301 cagtatgagt tgggttcgct gtcgcctgtt gcggcagtgg cgtgtcggcg cgccttgtcg
     3361 cgtttaggtt tggatgtgca gattaagtgg cccaatgatt tggttgtcgg acgcgacaaa
     3421 ttgggcggca ttctgattga aacggtcagg acgggcggca aaacggttgc cgtggtcggt
     3481 atcggcatca attttgtcct gcccaaggaa gtagaaaatg ccgcttccgt gcaatcgctg
     3541 tttcagacgg catcgcggcg gggcaatgcc gatgccgccg tgctgctgga aacgctgttg
     3601 gtggaactgg acgcggtgtt gttgcaatat gcgcgggacg gatttgcgcc ttttgtggcg
     3661 gaatatcagg ctgccaaccg cgaccacggc aaggcggtat tgctgttgcg cgacggcgaa
     3721 accgtgttcg aaggcacggt taaaggcgtg gacggacaag gcgttttgca cttggaaacg
     3781 gcagagggca aacagacggt cgtcagcggc gaaatcagcc tgcggtccga cgacaggccg
     3841 gtttccgtgc cgaagcggcg ggattcggaa cgttttctgc tgttggacgg cggcaacagc
     3901 cggctcaagt gggcgtgggt ggaaaacggc acgttcgcaa ccgtcggtag cgcgccgtac
     3961 cgcgatttgt cgcctttggg cgcggagtgg gcggaaaagg cggatggaaa tgtccgcatc
     4021 gtcggttgcg ccgtgtgcgg agaattcaaa aaggcacaag tgcaggaaca gctcgcccga
     4081 aaaatcgagt ggctgccgtc ttccgcacag gctttgggca tacgcaacca ctaccgccac
     4141 cccgaagaac acggttccga ccgctggttc aacgccttgg gcagccgccg cttcagccgc
     4201 aacgcctgcg tcgtcgtcag ttgcggcacg gcggtaacgg ttgacgcgct caccgatgac
     4261 ggacattatc tcgggggaac catcatgcct ggtttccacc tgatgaaaga atcgctcgcc
     4321 gtccgaaccg ccaacctcaa ccggcacgcc ggtaagcgtt atcctttccc gaccacaacg
     4381 ggcaacgccg tcgccagcgg catgatggat gcggtttgcg gctcggttat gatgatgcac
     4441 gggcgtttga aagaaaaaac cggggcgggc aagcctgtcg atgtcatcat taccggcggc
     4501 ggcgcggcaa aagttgccga agccctgccg cctgcatttt tggcggaaaa taccgtgcgc
     4561 gtggcggaca acctcgtcat tcacgggctg ctgaacctga ttgccgccga aggcggggaa
     4621 tcggaacata cttaaaaaat acaccctttt tcaacacggg gcggtatccg tccgaatccg
     4681 tccggattct gccgcccgac agactgccgc ccaggcgggc ggggtttcaa acattgagga
     4741 aattctaatg aaatggctct tcaccctttt ggcggtactc aatattgccg tattcggcgg
     4801 cacggtgggc tataaattgg ccgttaaagc gacgggcggc gtgccggaaa accgggccgt
     4861 cgaaaacacg ccccccgcaa cgcccgccgc cgacaatgcg gcggcatccg tggaggatac
     4921 ggcggcactg ctcaaaccgg gcgacatcct gaccgaagag caggcggagc agttgcgctt
     4981 gaagaaagaa gcggaacaga aaaaactgag ggagaaaaaa cagcgtgaag aaaaagcccg
     5041 ccgcgaaaaa ctcgccgccg aaaaggcgca ggcggaacgc gaaaacggcg cggcggatgc
     5101 cttatgcgcc gcgcaggcaa gcctcacgat ggacgaagat gactaccacc gcatcaaagg
     5161 acttttgggc aaatggtcgc acgttgccag caggagcgtc gaaaaacgca ccgcccaagc
     5221 caaacctgcc gacaaaacct accgcgtcgt cctgcccgtt tccgccgatg ccgaaaatca
     5281 ggcggcggag ctgtctgcca aaggtttcaa ccccataccg tttgacggcg cattgagttt
     5341 gggtgtcggc aacagccggg aaaacgccca agccctgcaa aaccggcttg ccggcgccgg
     5401 attcggcggg gcgcatattg tcgaacactt tgccgaagcc gacaggcagg acgattcttt
     5461 gagcgtgtcg cgtatgacgg ttttgtttac cggcgtgaat gccgccgatg cggaagaaat
     5521 tcgtaaaatc acgtccctat acggcaaact gaacctcaag tcttgcaaat aggcgcgaaa
     5581 gctggacgat aaacgccaaa cccaccgcga aggtgggttt ggcgtttcgg ttttgagtat
     5641 ctgatatata aaaagggaat gtatgtctcg tatatgccga ctccaagtgt gaaagtgaat
     5701 tgtgagggaa tacagtgttt aatggttcgg aatggacgac ttccgagtgg ttttgattat
     5761 acgctaataa taataattat caataaaaat ttaaaaataa atgcggattt tttgtaatac
     5821 gatgaaaaat aaacaaatta acctgtccgc cgatgtttgt gattcgggtc ggcaaacttt
     5881 cattgtgtcg gacaaatcgg ggtcggcagt catagctgag ggcttcgtgc ctgacggaaa
     5941 taagcctgcc gtgtttgaac atccgtccga tttggcgcgg acagggttgt cattcggggg
     6001 tgtttgagcg tggattcggg gatgcaaaag cggtaaaacc gttgcggatg aaggcttacg
     6061 gacggcttta ggtattttta agtgtattca aacaacaaca aaacgcttct tctattgccg
     6121 tatttagggc ttgcgcggca gcctgcacac cgtggaggcc gagtgtttcg ttggttgcgg
     6181 catcgggacg ggcggcaagg tttttcggcc ggtttttcca ttttttccga caaagccgtg
     6241 atgctgggga atggcggttt gtccgtttca ggccgccgcc gttttggacc gcgatgccgt
     6301 ctgaagcctt cagacggcat ttttgccgcc tttttcaata ttccgccgaa tgccaaaacg
     6361 gttgtccgac tgtcggcgtg ctggcttgcg ctttgtcgcg tgcttcgacc ggtccggctt
     6421 caaatgccag ccgtccggat tcgacggcga gtgcgaaggc gcgtgccata ttgaccggat
     6481 cgccgctgcg ggaaacggca gtattcaaaa gcacgccgtc aaagccccat tccatcactt
     6541 gtgccgcctg tgagggcaaa cccaagcccg cgtcgataat cagcggcgtg tcgggcaggc
     6601 gttcgcgcag gacgttcaac gcgtaggcgt gaaccgcgcc caaacccgtg ccgatcggag
     6661 ccgcccacgg cattaacgcc tgacagccca catcgagcag gcggcggcag gcaatcaggt
     6721 cttcggtgca ataaggcagc actttgaagc cgtctttaat caggatttcc gccgcttcga
     6781 caagttggaa cacatccggc tgcaaggtgt cgtcgtcgcc gatgagttcg agtttaatcc
     6841 aatcggtttc aaacacttcg cgcgccattt gcgccgtcgt taccgcttcc tgcacgcttt
     6901 ggcagcctgc cgtgttcggc aggacgggaa cgccggtttc ttgaagcagc gaccaaaacc
     6961 cctgaccgtg cgcctcgccg ccgcttcccg tgcggcgcag cgagacggta atcatcgcgg
     7021 gttgggcgat acggacggat tgtttgagga tttcaggggt cgggtaggca gccgtgccga
     7081 gcagcagccg cgaggggaaa gtttctccgt atagggtgag cataatgggt tcctttgtat
     7141 agtggatgaa caaaaaccag tacggcgttg cctcgcctta gctcaaagag aacgattctc
     7201 taaggtgctg aagcactaag tgaatcggtt ccgtactatt cgtactgtct gcggcttcgt
     7261 cgccttgtcc tgatttaaag ttaatccacc ataacggtgt tttggggaca ggggtcgtct
     7321 gaaaaggcaa aaccgcctag ccgccgacca ccggccgcac gatatcgatt ttgtcgtttt
     7381 cgtttaaaac cgtttccgca tacgcgcctt tggggatgaa aacggtgttg accgccacgg
     7441 caaagggctt ttgcggcgcg gtttgggcga tgaggtcggc aacgcttgtg ccgtgaagtt
     7501 cggcgggtcc gccgtttaag atgatgttca tggtttctcg atgtttctgt atttatagtg
     7561 gattaacaaa aaccagtaca gcg
//
LOCUS       DAWFJR010000095         7481 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWFJR010000095 DAWFJR010000000
VERSION     DAWFJR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.095"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(365..1120)
                     /locus_tag="WGR04_001768"
     CDS             complement(365..1120)
                     /locus_tag="WGR04_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ0795276.1"
                     /translation="MKFTRLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYRGVYDMHGLIWEWTEDFNSSLLSSGNADAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCASR"
     gene            complement(1247..2406)
                     /gene="nirK"
                     /locus_tag="WGR04_001769"
                     /pseudo
     CDS             complement(1247..2406)
                     /gene="nirK"
                     /locus_tag="WGR04_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
     gene            2777..5032
                     /locus_tag="WGR04_001770"
     CDS             2777..5032
                     /locus_tag="WGR04_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ0795277.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQTTGGMEVGSVLGHGAYQAPDWTADWLHRELVAWLDLTAQE
                     TYGKKFDEVSPEEQAVLKTRLADEYHNQSRIKEDGSVVVSETRVKAIESILPYYHGVY
                     SDDPKFQTTREHFAMKNNTLPSQEAREKLFNFFFWTSWSASTNRPDETFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFI
                     FYNMGFVRRSTATASTLAAGAIFMLGGIPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNVQFDDKLMTWGFWLLNGGLVGM
                     IVISLLPVGAIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLIFIGGAICVAI
                     QATKIVFGRDK"
     gene            5211..5744
                     /locus_tag="WGR04_001771"
     CDS             5211..5744
                     /locus_tag="WGR04_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ0795278.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5807..6991)
                     /locus_tag="WGR04_001772"
     CDS             complement(5807..6991)
                     /locus_tag="WGR04_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ0795279.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRF
                     QRFAGTDAQRAADFQDVASGRVAIPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGTLWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILGKQRAIIFGDFRMGNIRDVYDSSYDFSTVVNHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(7031..7435)
                     /locus_tag="WGR04_001773"
     CDS             complement(7031..7435)
                     /locus_tag="WGR04_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ0795280.1"
                     /translation="MTAQPCPICTAQNEDVLLQTPKLRVIAVHNDSGSPAFCRVIWHE
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFQPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         1702 a         1776 c         2074 g         1929 t
ORIGIN      
        1 gagattctag attcccactt tcgtgggaat gacgtggtgc aggtttccgt gcggatggat
       61 tcgtcattcc cgcgcaggcg ggaatctaga ctttagaaca acagcaatat tcaaagatta
      121 tctgaaagtt tgagattcta gattcccact ttcgtgggaa tgacgggatg taggttcgtg
      181 ggaatgacga aaagtggcgg gaatgacgaa aagttgcggg aatgacggtt cgggcattcc
      241 ttaaatcacc cgtgtatcgc tgtaaatctt agagatggcg gaatatagcg gattaacaaa
      301 aaccagtacg gcaaggcgag gcaacgccgt actggttttt gttaatccac tataattgaa
      361 ggggttatcg gcttgcgcaa cggaagccca agttgtgcaa gacatatttg gattgcaggc
      421 tggtgcggat gccgtagcgg aggaatgcgg catagttgga cgagtcgctc gatccgatag
      481 atgctccgct gcaaaacatt tgcgcatcgg cattgccgga agaaagcagg ctgctgttga
      541 aatcttccgt ccattcccaa atcagaccgt gcatatcata aacgcccctg tagttcgggc
      601 ggcctttgcc gacatcgtgc aggcctttcc ggccgccatc cgcataccag tcgagaatag
      661 tgcggttgta gccgggttcg tttgagccgt ttttctgcgt ggcggaagca agtccggcaa
      721 attcccattc gtcaatggtc ggcaggcgtt tgccttgtgc ggcgcaatag gcgttggcgg
      781 caaaccagga aacattggtt accggttgtt ttaattcgcc cgccttcggc gcatagctgc
      841 ggctgccgtt tttcatccaa tgcttcaggt aagcgggttc tgcctgtttg gaaccgatcc
      901 tgcctttttg ccattggggg tggctgttga caaattcggc aaactcggca ttggtaacgg
      961 gatatttatc cagtttgaac ggtttgactt taatcaggcc ggtatctttt ttcagataaa
     1021 gcgggcggta gctgccgcct tcgatttgaa ccatttcggc agctgccgct tgagtgccgg
     1081 cgagtgccgc acagagaaag agtaaccggg taaacttcat atcatgcctc ctgacaggca
     1141 gttaaaatca atcttccgaa aggaaagatt ggttgttaaa aaaccactgc cgtgcgtaat
     1201 gaagtacggc ggcagtggtt cgtttgccta aaaatgggtg ttcaatttag taatcgcttt
     1261 ttgcaggagc agaggctgca ggagcagaag ctgcgggagc ggaagcagca ggagctgcac
     1321 cgttaccggc gtaagcggta tcactcaatt tttgagtcat gatttcaggg ttttctgcac
     1381 cttctacttt caattgaccc agtgcgcctt tgttgaatgc gcggaagata gagtggtcaa
     1441 ccaaagtgta gctgcccggg atgtcgactt tgaattcgac gatggcagag ccgccggcag
     1501 gaacgatggt actttgtacg ttttcgttaa tcagtttgcc gccttcaaca taaactttgt
     1561 cgaagatttc accgattacg tggaaggaag acaccaagtt cgggccgccg ttaccaacgt
     1621 acatacgcac ggtttcgcct gctttggctt tcagggcgtt gtcgccggcg atagcaccta
     1681 cgtgaccgtt gaatacgacg tattcaggct gttcggcaac ggctttgtcc atatcgaacg
     1741 gttgcagacc ttgcgcgcct tttttgcctt tggtgtagaa gtcgccttgg acgatgtagg
     1801 actctttatc cactttcggc agaccttctt taggctcgac caaaatcaga ccgtacatac
     1861 cgttggcgat gtgcataccg accggcgcga cggcgcagtg gtagatgtac agacccggtt
     1921 gcagggcttt gaagctgaat gtggaagtgc ggcccggggc ggtaaaggtt gcggctgcac
     1981 cgccgccctg accggttgcg gcgtggaagt cgacgttatg gggaacggta gaagacgggt
     2041 tgttggaaaa ttccacttca accgtatcgc cttcgcgtac gcggatcatg cgacccggaa
     2101 cgtcgccgtc aaatgtccag tagcggtatt ccacaccgtc ttccatggtc atggtttttc
     2161 gacagtttcc atttttacgc ggactttggc aggatagtcg cggtcgattg cgggaggtac
     2221 ttcaggagcg tgggtggtaa ccgcatcgat aacgggcagt tcgcctgcag gcgtttcggc
     2281 ggcggtttgt gcggcggaac ttgcggcttc tgcggaagcg gcaggggctt gcgcggcagg
     2341 ttcgccgccg caggcggcta atgcgaataa ggaagcaatc attgcagcta aggcttggcg
     2401 tttcataata ttttcctttt gtaagaaaag tagagggatt ggttaagaac ttggtgtgat
     2461 tatgcgtttg ccccattggg ggcattttga tttaagtcaa ataattcata aaatatgaac
     2521 ttaaaatgat acttctaata ctttatagct actaaaaatt acaataaata ggctgatttg
     2581 cttaaggact tcggcaaatc aggcaaaggc agggtagttg ccgactaaag aataatgata
     2641 gttattatca tatatgtatt tgttttatat gtgagtttct ttgaacaaat gtttatttgc
     2701 gcggtaaacc gtgctacaat ctttaacatt catattttgt gaattttaat ccactatatt
     2761 aaaaggagct ctcaaaatgg gacagtacaa gaagctgtgg tacttgctgt ttgccgttct
     2821 ggcggtatgc tttaccattc ttggctatat gggcagcgag gtttacaaaa aagccccgcc
     2881 ttaccccgag caggtcgttt ccgcatccgg caaagtcctg atgacgaaag acgatatttt
     2941 ggcaggtcag tctgcatggc agactaccgg cggtatggaa gtcggctctg tattgggtca
     3001 cggtgcatac caagctccgg actggactgc cgactggctg caccgtgagc tggttgcatg
     3061 gttggatctg actgcgcaag aaacttacgg taaaaaattt gacgaagttt ctcctgaaga
     3121 acaagctgtt ctgaaaaccc gtttggctga cgaataccac aaccaaagcc gtattaagga
     3181 agatggttct gtcgttgtca gcgaaacccg cgtgaaagcg atcgaaagca tcctgcctta
     3241 ctaccacggt gtgtacagcg acgatcctaa gtttcaaacc actcgcgaac actttgcaat
     3301 gaaaaacaat acattgccaa gccaagaagc gcgtgaaaaa ctgttcaact tcttcttctg
     3361 gacttcttgg tctgcttcga ccaaccgtcc tgacgaaact tttacctaca ccaacaactg
     3421 gccgcatgag cctctgatca acaacgtacc gactactgaa aactacatgt ggtcgtttac
     3481 cagtgtcgta ctgctcttga tgggcatcgg tttgttgatg tggggttatt ccttcctgac
     3541 caaacacgag gaagtggaag tgccgtctga agacccgatt tccaaaatcc aactgacccc
     3601 ttcgcaaaaa gcattgggca aatacgtctt cctgacggtt gccctgtttg tggtacaagt
     3661 attgctgggc ggtctgactg cgcactacac cgttgaaggt cagggtttct acggcattga
     3721 cgaggcgttg ggctttgaaa tgtccgactg gtttccctat gccctgaccc gtacttggca
     3781 catccaatcc gctatcttct ggattgcaac cggctttttg acggcaggtt tgttcctcgc
     3841 gccgattgtc aacggcggca aagatcccaa gttccaacgt gcaggcgtga acttcctcta
     3901 tatcgccctc ttcatcgtgg tcggcggttc ttacgcgggc aacttcttcg cgttgacgca
     3961 catccttccc cccgaattta acttctggtt cggacaccaa ggttacgaat acctcgattt
     4021 gggccgtttc tggcaactcc ttttgatggt cggactgctg ttgtggctgt tcctgatgtt
     4081 gcgctgcacg gtttccgcct tcaaagaaaa aggcgtggac aaaaacctgc tggcaatctt
     4141 tgtcgcttct atggtcggtg tgggcgtatt ctacgcaccg ggcctgttct atggcgaaaa
     4201 atccccgatt gccgtaatgg aatactggcg ttggtgggtg gttcacctgt gggtggaagg
     4261 tttcttcgaa gtgttcgcta ccgctgcctt cgcatttatc ttctacaaca tgggctttgt
     4321 ccgccgtagt accgctactg cttctaccct ggctgcaggt gctatcttca tgctgggcgg
     4381 tatcccgggt acgttgcacc acctgtattt ctccggctct acttctgctt ctatggcaat
     4441 cggtgcctgc ttctccgctt tggaagtcgt gccgctggtg ttgctgggtc gtgaagcata
     4501 cgagcattgg tcttaccaac acctgtccga ctgggcgaaa cgcctgcgtt ggccgctgat
     4561 gtgcttcgtg gcagtcgcct tttggaatat gatcggtgcc ggcgtattcg gcttcctgat
     4621 caatccgccg atttccctgt tctatattca aggtttgaat acttctgctg ttcatgcgca
     4681 cgctgccttg ttcggtgtgt acggtttctt ggctctgggc ttcgtattgc tggttgcccg
     4741 ttatttgaaa ccgaacgtgc agtttgacga caagctgatg acttggggct tctggttgct
     4801 caatggcggt ttggtcggca tgattgttat cagcctgctg cctgtcggcg cgattcaggc
     4861 atacgcctcc atcacgcacg gtctgtggta tgcccgcagc gaagaattcc tgcaaatgga
     4921 aatcctcgac accctgcgct gggttcgtac tgccgctgac ttgatcttta tcggcggtgc
     4981 aatctgcgtt gccatccaag ccactaaaat cgtattcggc cgcgacaaat aagttttgcg
     5041 gtatgaaatg agcaactgca cccgttttcg ggtgcagttt tttattgcgg attgggacgg
     5101 gaatggaaaa ggatgtggat tttttatttt aatcggctat acaattgctg ctttcatgcc
     5161 gtctgaagca cgtttcagat ggcatcggtt tacatcagat aaggataaaa atgggtattt
     5221 acgattttca aatgaaagat gcagaaggca atgcggttga tttgtcaggc tatcgcggca
     5281 aggttctgct gattgtcaac acggcaacgc gttgcggtct gaccccgcaa tacgaggctt
     5341 tgcagaagct gtatgcacaa tataccgcag aaggcttgga gattttggat tttccgtgca
     5401 accagttccg cgaacaggct cccgaaagca gcggagaaat tgcccaagtg tgtatgatga
     5461 agtttggcac gaagtttaag attttcgaca aaatcgaagt caatggagca aataccgcgc
     5521 ccttgtatgc ctacctgaaa tccgtcaaac cgcaagataa aggcaatcat ctgtttaaag
     5581 atttcgtgtt gaagctcgca gctttgggag agaagcgcga cgaaggcgac atcaaatgga
     5641 actttaccaa attcctcgtc aaccgcgacg gcgaagtggt cgagcgtttt gcgcctagtg
     5701 ttacgcccga agaaatcgaa gccgatattc gggcattgct gtaatctgcc gccgtcttgg
     5761 gaaatgccgt ctgaaggctt cagacggcat ttgtccgttt gcgggtttat tccgaaatat
     5821 cggcaacacc gctttcgggg aagatgggca aatccggcgg tgggagcagg gtatccaaag
     5881 tcagggcgga cgcatcgagt gtggggtagc cttcaaacgc gaccgaataa ccgctgtttc
     5941 cgttcatacg gattcgggcg tgcgcgccca gcgggaaagt gattttgtcg gcaatgtgcc
     6001 cgaacgggaa acctgtcagc acaggaattt ttgccgtacg cgaaacatgg ttgaccacgg
     6061 tagaaaaatc ataagacgaa tcgtatacat cgcggatatt gcccatacgg aaatcgccga
     6121 aaataatggc gcgttgtttg cccaaaatac ccgaaagata cagcgtattg agcatacgtt
     6181 cgatgcggta gggctgttcg ccgacatctt cgagaaacaa aatgccaccg tcgatgtcgg
     6241 gcatataagg cgtaccggcg agcgaggcga ggacgcttaa gttgccaccc cacaacgtgc
     6301 cttcggtttc gacatcggcg cgttggatat aaggaacatc aacggtcagg cggttttggg
     6361 ttgccccctt gataaaggca tccatcgtaa acgcaccggg ggcaggtttg ccaaaatcgc
     6421 tataagccat cggaccggca aaactcatca tattgccttt tgccaataat gccagttgga
     6481 cggcgcaaac atcgctgaag ccgaaaaaca gcgtaccgtg ttcgcgcatt cttgcgccga
     6541 gcgaggcaaa atcgatatgc ggcagaatgc gtgctgcacc ataaccgccg cgcaaaccca
     6601 tcagaacttt cggtatggca acgcggccgg aagcgacatc ttggaaatcg gcggcacgtt
     6661 gtgcgtccgt accggcaaac cgttggaagc ggcggctgcc cgcctgttgg ttggttacgg
     6721 taaaaccggc attgtaaagg cgggttaagg ctgtgttgac gcggttggtg tcttcggcaa
     6781 atcctgacga agcgacaacg cgcagaaggt tgccgtccga actttgacgg cgtggcgttt
     6841 ggagaggcac ggttcgggct ttcacaacgg aatgggaaga gggaaggggc ggaacggatg
     6901 tggcggatgt gccgcaagcc tgaagcagcc ccgcgccggc agcggcggtg caggttttca
     6961 gaaaacggcg gcgggaggtc ggttcggtca taatttccct ttcatacggt ttcagacggc
     7021 atcggcgggc ttaaagcagc tttttaagct gttccgtcca atcttgcggc agggtcatac
     7081 cgtgtttccg gacggggttt gcccaaatcg gcgcggggaa ggacgcatcg ttttcaaagc
     7141 gggcgataat atgccaatgc aggtgcggca cgacattgcc caagctggcg aggttaatct
     7201 tggcaggttg aaacacttgg cgcatagcgg cttcgacttt gtacaccatt tccatcaatt
     7261 cgccgcgttc cgctgccgaa aggtcggtca tttcggcgat gtgttcatgc cagataacac
     7321 ggcagaatgc aggcgaaccg ctgtcgttat ggacggcgat gacgcgcagc ttgggggttt
     7381 gcagcaaaac gtcttcattt tgcgccgtgc agatggggca gggttgggcg gtcatttttc
     7441 cttattcctg aaagtgttta aaaaacggta ggataatttc t
//
LOCUS       DAWFJR010000096         7405 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWFJR010000096 DAWFJR010000000
VERSION     DAWFJR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7405
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.096"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(304..1716)
                     /gene="trkA"
                     /locus_tag="WGR04_001774"
     CDS             complement(304..1716)
                     /gene="trkA"
                     /locus_tag="WGR04_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ0795281.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGEVIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(1814..3337)
                     /locus_tag="WGR04_001775"
     CDS             complement(1814..3337)
                     /locus_tag="WGR04_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ0795282.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMNVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPRKWQAKIGIIPVES"
     gene            complement(3644..4450)
                     /locus_tag="WGR04_001776"
     CDS             complement(3644..4450)
                     /locus_tag="WGR04_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ0795283.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKVSSSEDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPAKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            complement(4502..4753)
                     /locus_tag="WGR04_001777"
     CDS             complement(4502..4753)
                     /locus_tag="WGR04_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795284.1"
                     /translation="MSSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEVA
                     VESIQLAFEDALAELNYEDSPHTDLFHDVTYAFFREKER"
     gene            4853..5614
                     /locus_tag="WGR04_001778"
     CDS             4853..5614
                     /locus_tag="WGR04_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795285.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRARVLKAYLQKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRILFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMALFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            5759..6928
                     /gene="metZ"
                     /locus_tag="WGR04_001779"
     CDS             5759..6928
                     /gene="metZ"
                     /locus_tag="WGR04_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ0795286.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VVSSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAGYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQEAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            7024..7230
                     /locus_tag="WGR04_001780"
     CDS             7024..7230
                     /locus_tag="WGR04_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ0795287.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT         1582 a         1843 c         2157 g         1823 t
ORIGIN      
        1 tttgaatatt gccgttgccc gacgttctgg attcccgcaa cttttcgtca ttcccgcgaa
       61 agcgggaatc tagaacctct aaattttcag ataacctttg aatattgccg ctatcccaag
      121 gtctggattc ccgcctgcgc gggaatgacg ggtcttttat aacctttgaa tattgccgct
      181 atcccaaggt ctggattccc gcttacgcgg gaatgacggg gagggcggat gccgtctgaa
      241 gcccacctac cattatttcc gcttccatac cgcttggcgg caatatgtcc ggaatggggc
      301 ggtttatccg aaaaagccca ttttgacttg gatgagtttt tccagttcgt tcaggatgcg
      361 ccggcgcgag acgaaaaaga tgatgtggtc gccgtcttgg atgacggttt cggtatggtg
      421 tcccataatg acttcgccgg ttccggcgcg gacgacggcg gcaatgtggc agccttcggg
      481 ccatttgatg ccgctgatgc gcctgccgat gatggcggaa gtttttttgt cgccgtgtgc
      541 gacgacttcg atggcttccg ccgtgccgcg ccggatgggg tggacggcaa cgatgtcgcc
      601 gcgccggatg tgggcgagta tcgagccgat ggtgatgagg tggggggaga cgacgatgtc
      661 gattttgttg ccttcgagca aatcgacgta gcttgagcgg ttgacgatgc cgatgacgcg
      721 cttcgcgccg aggtttttcg ccaaaagggc ggacataatg ttgctttcgt cgtcgttggt
      781 cagggcgcag aatacgtcga tttcgtcgat gtattcgttg tcgagcaggg tttcgtcggt
      841 tgccgaacct tgcaggacga gggtgttgtc gaggttttcg gctatccatt cggcacggcg
      901 cggccggcat tcgatgattt tgacgttgta tgcgtgttcg agctgcttgg cgagacggta
      961 gccgatgttg ccgccgccgg caatcatgat gcggcgggtg ctggtttctt tggggcgcaa
     1021 ttcgggtatg accgcgccga tgttttcggc ggcggcggca aacaggattt cgtcgccttc
     1081 gatgatgacg gtttgcggcg cggggacgat gaggcggttg ttgcggtaaa cggcgcagat
     1141 ttggcagtcg gccccgtcgg gcaaatcttg ggcgatgtcg gcaatgctgc gtccgacaag
     1201 cagtccgccg cgccgcgcct gtatgatgac catccgcacg cggtcgtctg caaaacgtaa
     1261 aacctgcaat gcgcccgggc agtctatcag gccggcaagc tgttcggtaa cgagctgttc
     1321 ggggctgatg gtttcggtta tgccgaatat ggaaaggctg ccgttttcgt tgttttcgag
     1381 cttggggctg aggtattcga ggtattcgct ggaacggacg cgcgcgatgc ggccggggat
     1441 gttgaacagg tcggcggcaa ctttgcaggc gacgatgttg gtttcgtcgc tgcgggagag
     1501 cgcgagcagc aagtcggcat cttccgcgcc ggcgcgttct aatgtgaagg gggatgcgcc
     1561 gttgccgaaa acggtttgga catcgaggcg gctgcctgtt tcctgcaatg ctttttcgtc
     1621 gatgtcgata acggttacgt cgttgttggg tatggcggca aggttttgtg cgacggtaga
     1681 acctacctgt ccgttgccta aaatgaggat tttcacggtg tgggtcgccg agtgatgaaa
     1741 tggtggattg taccgcaacc gggggcggaa atgccgtctg aaggcagatt ttgtgttcag
     1801 acggcatggc gcctcaagat tcgacgggga tgatgccgat tttcgcctgc catttgcgcg
     1861 gggcggtggc gtggatggat tcgcctttgc tatctacggc gacggttacg ggcatgtctt
     1921 tgacttcaaa ttcgtaaatg gcttccatgc ccaattcggg gaacgccaag actttggaag
     1981 atttgatggc ttttgccacg agatacgccg cgccgccgac tgccatgagg tacacggctt
     2041 tgttgtcggc gatggcttcg caggtggcca cgccgcgctc ggatttgccg atcatgccca
     2101 agaggtcggt ttgttcgagc atttggcggg tgaatttgtc catgcgggtg gctgtggtcg
     2161 gacctgccgg accgacgact tcatcgccga ccggatcgac gggaccgacg tagtaaatca
     2221 ggcggttggt gaaatcgacg ggcaattctt cgcctttgtt gagcatatcg acgaggcgtt
     2281 tgtgtgcggc atcgcggccg gtgaggattt tgccgttcaa cagcaatacg tcgccggttt
     2341 tccagcttgc cacttcttct ttggtcagct tgtcgacatc gacgcgtttg ccgttgtcgg
     2401 ggctgtaagt caaatcgggc cagtcttcga cgcgcggcgg cgtgagttcg acagggcctg
     2461 agccgtccaa ttcaaattcg acgtggcggg tggcggcgca gttcggaatc atggcaatcg
     2521 gtttggaggc ggcgtgggtc ggataatcga ggattttcac gtccaacacg gtggtcagtc
     2581 cgcccaagcc ttgtgcgccg atgcccagcg cgttgacttt ttcaaagagt tcgaggcgca
     2641 gggcttcggt ggtggacaat tccgcgccgg acgcggcctt ttcctgcaat tcttgaatgt
     2701 cgatgtggct catcagggac tcttttgcca tcagcacggc tttttcgggc gtgccgccga
     2761 tgccgatacc caagatgccg ggaggacacc agcccgcgcc catggtcggg atggttttca
     2821 atacccaatc gacgatgttg tcggaaggat tgagcatggc gagtttggat ttgttttcag
     2881 agccgccgcc ttttgccgcg caggttactt cgactttacc gcccggcacg atgctcatat
     2941 ggatgacggc gggggtgttg tctttggtgt tttggcgttt gccggccgga tcggcgagga
     3001 cggaagcgcg cagcgtattg ccttcccaag tgtaggcgcg gcgtacgcct tcgttaacca
     3061 tctcttccac gctcatgtcc gcatcccatt ggacgttcat accgactttg aggaagacgg
     3121 ttgcgatacc tgtgtcttgg cagatggggc ggttgttttc cgcacacata cggctgttga
     3181 ccaaaatctg cgtcatcgcg tctttggcgg caggattttc ttccttctgc cacgccttat
     3241 aaagcgcgtc gatgtagtct ttgggatgat agtagctgat gaattggaag gcatcgcaaa
     3301 tgctttggat aaagtcttcc tgtttgatga cggtcatgat tgtgttcctt ttcaaggttg
     3361 tggcggtaag tgtctgctgc tgttttaaac aacctttttg aaggctgttt tttatcaggt
     3421 tgaaatagtg ccagcgtttg tacggtaaag acaatacata attctaatct cttgattatc
     3481 aatttgtttt aataaaatac tgttttactc ggattttgat atttgtaatc tttgcctgtt
     3541 gctttgcggt agggagaaat cagcaggcag ttcattgtag gccgattgat gaacaatgcc
     3601 gtctgaaagg gcttcagacg gcattgtgtt cggacgggcg ggcttatttt gcggcctgtc
     3661 cgtcttcttt tgcaaaatat ttggcgtaaa tcttgtcgta ttcgccgctt tcgcgtactt
     3721 tttccaacgc atcgttcagc attttgacgg ttgcttcgtc gcctttgcgt accgcgatgc
     3781 cgtagtgttc ggtggtgaag tcgggcaggg taacgaagtc catccctttg gccggattgt
     3841 ttttcacata attggcgatg accgcgctgt cgctgaccac ggaatccaag ccgccgtttt
     3901 ccagttcttt gataatcagg ggaacgtttt caaagcgcgc gattttcgga ttgtcgttgc
     3961 ccaagagttt ggatacggag aaatcgcccg tgtagccggt taccacgccg actttgttca
     4021 tgtttttcaa atcttcggaa gaagatactt ttttgccttt cggaacgagg acgacttggg
     4081 tgatttcaaa atacgggtcg ctgaagtcca tagactgttt gcggtcgtcg gtaatggtta
     4141 cgcccgacat cacaacgtcc gcatcgccgt tgtttaaggc ggggaaaagg ctgtcccacg
     4201 gctggtgttt gaattcgatt ttaaaattgc ccgccttcgc catcgcgttc atcaaatcca
     4261 catcgaaacc ttcgacattg cctttcgagt ctaaagattc aaagggggca aactcggcgt
     4321 tggaagccac gcggtacact ttgtcggggt ttgcggcggg cgcggcggca tctttgccct
     4381 gaccgccgca ggcagacagc gcgagcgcgg aacaggcaag ggcggcggca atccattttt
     4441 tcatattcat caaaattcct ttcggtaatc aaaaagccta aaaaatcagt ttaacacaaa
     4501 attaacgttc tttttcacgg aaaaacgcat aagtaacgtc gtggaacaaa tccgtatgcg
     4561 ggctgtcttc gtagttgagt tccgccaaag cgtcttcaaa tgcaagctgg atggactcga
     4621 ccgcgacttc ggcggtgtcg gcgggaaggg cgcggatcag ggctttgaag gcggtggaca
     4681 ggacgtggtt ctgcatacgc agcacgtcgt ttgcctcttc cagatattcc aaacgctgtt
     4741 cgatgctgct catgatgtat cctcgcgaat ctgttaaaat gcatcattat atcaaaccgc
     4801 gctttgggcg cgggtttgtc tgcttgcaaa gattaagaac cggaagccga aaatggatgc
     4861 gaataaagag ccgaattggg aaaaacgttt ggaaaacctg cgtcagacgc gccgtatgaa
     4921 ggcagtgccg acttacgcgc cgctgtcccg cccctctgaa accggaacgg acgcgcctgt
     4981 gccgaaactg gacgatgaga gccgagcccg tgtgttaaag gcgtatctgc aaaaatggca
     5041 ggaggaacac aatgccgccg ttgccgattg cggcgaggaa gtcgaaaccg acccgatgat
     5101 tgtccttcag gaaaactggc tcaacgcgca ggcttccctg caaatgtcgg tttcggaaaa
     5161 acacatcgaa tcgcggcgga gaatcctgtt cgatgccaaa aaagacagtg ccgaatttga
     5221 aaccggacag attgaaggcg gggaaaacgc cgatgcggca gaatcgggta cggcggcaga
     5281 cggggttgcg gaaaacggag aaggcggtgc ggaagaggcg ttgatgcccg tccaaatcaa
     5341 tatcctgaat ccgaaagccg tcaaccggcg cgaagtgttc tgcctgtccg aacaggagct
     5401 gaccgaacgg ctgatcaaac gcctgcgtcc gcatctgacc gataccgtca acggcatcat
     5461 tcgaacggcg gtgcaaaagc agatggcact ctttacttat cagctccagc aaatgctgca
     5521 cgagcaggcg ggcgcggtgg tcgaagacgt gttggaacac gatgtccgca aaatcctcaa
     5581 cgacatcaag tacgagttaa aatacaaacg ctgattgcca tgccgtctga aagccgtttc
     5641 agacggcatt tgcattctca tccgacttga aagcatcata gaacaggcgc aaaatattaa
     5701 caaattaaac aacgggcgca cgccccgttt ttgaacggac aaaaccgagg aatcaccgat
     5761 gagcaaaaaa ctccatccgc aaacgctcgc catacgcggc ggcaaagaac aaaccgaata
     5821 ccgcgaacac aatcaggcat tgttcctgac cagcagcttc atgtgggaca acgcccaaca
     5881 cgctgccgat ttgttttcaa aaaaaatcaa aggcttcact tatacccgta ccgccaaccc
     5941 gaccacagcc gcctttgaaa aacgcatcgc cgctttggaa ggtgcggaac gcgcggtcgc
     6001 cacttcgaca ggtatgtccg cgattcaggc ggcgtttttt accttcctgc aggcgggcga
     6061 ccacgtggtt tccagccgca gcctgttcgg cacgaccgtc ggctttatca ataacatcgt
     6121 taccaaattc ggcatcgaag tgagccacgt ttcgccgact gatataaacg aatggaaagc
     6181 cgccgtcaaa gccaacacca aactgctgtt tttggaaacg ccgtccaacc ccttgggtga
     6241 agtggccgac ttggaagcct tggcggaatt ggcgcacggc atcggcgcgc tcttggtggt
     6301 ggacaacagc ctgctgtcgc ccgtcggctc gcagcctttg aaacacggcg cggatatttc
     6361 cgtttcctcc gcgaccaaag ccatagacgg acacggacgc gtgatgggcg gcgtgttggc
     6421 gggttcggaa gaactgatgg cgcaggtcgc cggttactgc aactcttgcg ggctggcaat
     6481 gtcgccgttt aacgcatggc agttgttgag cggcgtagaa accctgtcgc tgcgtatgga
     6541 aaaacagttt gacaacgccc tgaaaatcgc gcaatggctg caagcgcagc cgcaggttca
     6601 agccgtgtac tacaccggat tgcccgacca tccgcaggca gagcttatcc gcaaacagca
     6661 aaacggcggc ggcatcgtca tcggctttga agtcgccgac caagaagccg cgtggaaagt
     6721 cgtggacggc gtggaactct tttcccgtac cgccaacctc ggcgacgtgc gctcgaccat
     6781 cacccatccg tggacaacca cgcacggcag gatgcagccc gaagaaaaac tcgccgccaa
     6841 catccgcccc ggattggtgc gcctgtcggt cggtttggaa tatgttggcg atttgattga
     6901 cgatttgaaa caggcactcg cgcgctgatt gcgtgtttca gacggcatcg gcggaaaaac
     6961 accgcaaatg ccgtctgaaa cccttatctt atatggcata cacgtcaaca ataggaaaaa
     7021 aatatggatg aagcggtttt tgccgattgg gcgttgaaaa tctgcctgac cggcttgatt
     7081 atttttttgg gttttatcgt ttggaatttg ggcaaggagt ccaaagcagg caaattcggc
     7141 atcgccattt tgtttttggt tttgggcttg ggcgtgttcg gttttgtatt taaagaactg
     7201 ctaatcaagt ttttggtatt gcccaaatag gttgccgcct gtcaaaatgc tgtctgaaat
     7261 cataaaaagt aatctgcacc ctaaaagttt gatttaaccg ccaactgatt aaggtgcgga
     7321 tttttatgac aaaataaaaa tcatctttcc gcagtcattc ccacggacgc gaaagtctgt
     7381 ctttttgaat cccggataat tttac
//
LOCUS       DAWFJR010000097         7340 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWFJR010000097 DAWFJR010000000
VERSION     DAWFJR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7340
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.097"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(172..2775)
                     /gene="pepN"
                     /locus_tag="WGR04_001781"
     CDS             complement(172..2775)
                     /gene="pepN"
                     /locus_tag="WGR04_001781"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ0795288.1"
                     /translation="MSKIVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASAGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEYSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNSFELTIKQTVPPTPDMADKQPIMIPVKVGLLNRNGEAVAFDYQGKRATEAVLLLT
                     EAEQTFQFESVTEAVVPSLLRGFSAPVHLNYPYSNDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGT
                     ENIDPLRYHQAHEALLDTLAAHFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2911..3705)
                     /locus_tag="WGR04_001782"
     CDS             complement(2911..3705)
                     /locus_tag="WGR04_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0795289.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWDAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            complement(4455..5324)
                     /locus_tag="WGR04_001783"
     CDS             complement(4455..5324)
                     /locus_tag="WGR04_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ0795290.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIADLTGSLAYFLVKPRRRIGEIN
                     LAKCFSEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKMLDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5375..5911)
                     /gene="ruvC"
                     /locus_tag="WGR04_001784"
     CDS             complement(5375..5911)
                     /gene="ruvC"
                     /locus_tag="WGR04_001784"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ0795291.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADAS
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(5914..6153)
                     /locus_tag="WGR04_001785"
     CDS             complement(5914..6153)
                     /locus_tag="WGR04_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ0795292.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6184..7194)
                     /gene="dusB"
                     /locus_tag="WGR04_001786"
     CDS             complement(6184..7194)
                     /gene="dusB"
                     /locus_tag="WGR04_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ0795293.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFADEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVKAAGVPVTLKTRLGWHDDHKNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEAAYDLIAETKCRLNIPVWVNGDITSPQKAQAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNHVRAMHAFYGETAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAGKTDRWACAYRQN
                     AV"
BASE COUNT         1640 a         1944 c         1911 g         1845 t
ORIGIN      
        1 gtactatctg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata
       61 attaatatga ctttgcggcc gttttgccat tgcgtaataa aacgatgggg aagtgatgat
      121 aaaacgtgtg tgtaactata tcagacggca ttgtttttct gtttgacggc ctcaatccaa
      181 aattttgccg acgatttcgc ccacgtcttt cgacaatcct tcctgcgccc gaatgcgctg
      241 caatgcttgt ttcaccaagt ttttgcggtg cggttcgagc ttattgcaga ggttgaacgc
      301 ctgcactagg cgggcggcga cctgcgggtt aaagcggtcg atttcgatga ctttgtcggc
      361 gatgaagcgg tagccgctgc catcttctgc gtggaaatgc gggacgttgc ggctgaagct
      421 gccgatgagc gaacgggctt tgttggggtt ttcgaggctg aatttcggat gctgcaaggc
      481 ggtttggacc tgttgcaggg tgtcgctgcg gcggcttgag ccgacgaggg cgaaatattt
      541 gtccatcacc agcgcatcat ccgaaaactt gtcggcaaac tgcgccagca ggcggttgcg
      601 cgtatcgctt tcgttgccgt tgacggcgga caggatgccc cattcgtggg tcatgttttg
      661 cgccatttcg ccgtatttct cggcaacggt ttcgatgtgc gcggggtcgg cgcgcaggac
      721 gaaggcgcgg cagacgttgc gcagcgtgcg ccagccggcg gcttcggggc tgtattcgta
      781 actttggttt tcctgcttcg ccgcctgacg gttcaattcg tgccatttcg gcaggaagtg
      841 ggcggcaagc gtgtccaaca aggcttcgtg cgcctgatgg tagcgcaacg ggtcgatgtt
      901 ttccgtgccg tcccacagct cggcttcaga cggcacgccc aaaagcaggg ctttgaaggc
      961 gttgtccaag aggtcgtctg aaatgacttt ttcgacggcg gcaagcagtt tttcgtgttt
     1021 cggcaacccg atgccgtctg aaagtgcggc aaggttggcg gcgacggcac ggcggtagag
     1081 cgtttgggcg gcttcccagc gcgtgaaggc gtcgctgtcg tgggcgagca ggagcagcag
     1141 gtcgtcgttg ctgtacggat agttcagatg cactggcgcg ctgaacccgc gcagcagcga
     1201 gggaacgacg gcttcggtta cgctttcgaa ctggaaggtc tgttcggctt cggtcagcag
     1261 taacacggct tcggttgcgc gtttgccctg ataatcgaat gccaccgctt cgccgttgcg
     1321 gttcagcagc ccgactttga cgggaatcat tatcggctgc ttgtccgcca tatcgggcgt
     1381 gggcggcacg gtttgtttga tggttaactc gaaactattg tttttcagac gaccttccgc
     1441 ttccaagacg ggcgtacccg cctggctgta ccacaaggcg aactggtcga gattgatgcc
     1501 gttcgcgtcc gccatcgccg cgcggaaatc gtcgcaggtc acggcctgcc cgtcgtgtcg
     1561 ttggaaatag agcttcatgc ctttttggaa gccctcttcg ccgagcaagg tgtgatacat
     1621 ccgcaccact tccgcgcctt tttcataaac ggtcatggtg tagaaattgt tcatctcctc
     1681 atagctggcg gggcgcaccg gatgggcggt cgggcctgcg tcttcgggga actggtgctg
     1741 gcgcagcagg cggatgttct cgatgcggcg cacggcgcgg ctggcgcggt cgccggaaaa
     1801 ttcttggtcg cggaacacgg tcaacccttc tttcagcgaa agctggaacc aatcgcggca
     1861 ggtaacgcgg ttgcctgtcc agttgtggaa atactcgtgt ccgaccacgg attcgatgcc
     1921 ttcaaaatcg gtatcggtgg cggtacggct gtcggcaagg acgaacttgg tattaaaaat
     1981 gttcagcccc ttgttttcca tcgcgcccat attgaaatcg cccacggcga cgaccatgaa
     2041 aatatccaag tcgtattcca aaccgaagcg cgtttcgtcc cacttcattg cgtttttcag
     2101 cgattccacg gcaaagccga ccttgggctt gtccgcttcg gtggtgtaaa actcgatttt
     2161 gacgtttctg ccgctcatgg tggtgaaata gtcttccgtg accgccaaat cgcccgcgac
     2221 caaagcaaac agataactcg gtttggcaaa cgggtcttcc catttcaccc aatggcggcc
     2281 gtctgaatac tcgccgccgt cgattttgtt gccgttggag agcaaaacgg gatagcgttt
     2341 tttgtccgcg acgatggtgg tcgtgaactt ggacatgaca tccggacggt cgatatagaa
     2401 cgtgattttg cggaagccct ccggctcgca ctgggtaaac aggttaccgg cggacgcata
     2461 cagccccatc agcgatttgt tttccgccgg caggatttcg gtttccactt cgacggtgaa
     2521 gcgttcggac ggcacgtctg caatcgtcag cgtctcacct tccaacacat aatccgccgc
     2581 cgccccgttg attttgacgg acaagagttt cgccgaaccg tccaacacca gcggctcccc
     2641 taccctctgc ggctcaaccg tcaaacgcga cttcacgacg gtttgcggtt cggcaatatc
     2701 aaaatgcagg tcggtttcaa ggatgcggta ggcaggcgtt tggtaatctt tcagataacg
     2761 gacgattttg ctcatttttt tctttcaata ttgtttggtt tgactggaaa aggcttcaga
     2821 cggcataggc aaaccctgca ccgatatgcc gtatgaaacc gaaacatcag tgcggacgca
     2881 ccgtcaaaca accggtttta atttcaatct ttatttccac gcttggacaa attctgccca
     2941 atgcggtttg tcttctgttt gggcggaaag atagtttctg atccgcttga tttccatttc
     3001 gtattcgtcc gcgtcgaatc tgccgctgac cagacagaaa cgcagataca tcaaatacgt
     3061 attcacggca tcggtttcac aataattgcg gatttccttc agcctgcccg cgtggaacgc
     3121 atcccacacc ttgctgccgt ccatacccag cttgcctgga aaaccgcaca gtttcgccat
     3181 atcgtccagc ggcacgtttg ccctcggctg gtaaagcgcg agcaaatcca tcaaatcgca
     3241 gtggcgttgg tggtaacggc tgatgtagtt gttccacttg aaatcgcggc tgtcgccgaa
     3301 atctccgtcg cccatatccc aatagcgcgc ggcgttgatg ccatatatca gggagcggta
     3361 atgcagaacc ggcagatcga aaccgccgcc gttccaactg accagttgcg gcgtatgttt
     3421 ttcaatcaac tcgaaaaatt tagcaatgac cacttcctcg ccgtcatcca tctcgccgat
     3481 ggtgccgaca tgtactttat cctgccccca acgcatacag cacgaaatcg ccacaacctg
     3541 atgaagatga tgctgcataa aatctccgcc cgtctgagca cggcgttttt gctgggcaaa
     3601 cagcaccact tcgtcgtcgg gcagcgagga cggcagctcg tacaatgttc ggataccctg
     3661 gacatcaggt acggtttcaa tatcgaaagc caaaatcgta ttcatggcag taccttgcat
     3721 tcaaaaacag acttgcgcct attgtgtcat taaaaggcgg ataaaaaaag aggcaacccc
     3781 ccacaggatt gccccaatac ctcaaatcag agatttacgc ttcacaaaca atacaggctt
     3841 tcgcctgcgg ctttacccgc gtagctcaac tctacgccgg caaactttcg tttcaccgtt
     3901 tccaatgaaa ccccgaccaa tcgcaagact gaccggaaaa tcctttcaga cggcatttcc
     3961 tgcctgtcgt gtaattccat gtagcgaaat gtacgccatt ttctacgctt tgccaagcat
     4021 tttttacaat ataaatgtca aaacattaat tttataaaat tgctgaaaat attaaatata
     4081 tggattttta ttttttatat ttcaataaat ataaatttaa ttttgattta tatttaaatt
     4141 taagcataaa atgtcaaata ttaaagtaaa catgaaaggc atatattaaa tatttattta
     4201 taacgctatg tttttaaaga aaattaattt taatatatta actagattgt ctgcatatat
     4261 tcataggttt gcggtatttc ttccaaaacc tgcttcgaat ttcccgacca agtcttaaaa
     4321 atattgtttt tgagatactt aaatagcagc gattatcaaa tgaaatctgt tcatataatc
     4381 tgccattttg catttaaaaa aacaatcagg agtttcgact cgaaacgcct gatatgtttt
     4441 gtaattttac gtagtcagta aaaatcgggg ctgccttccg gacgggtttt aaaacgcttg
     4501 tgcagccaaa aatactgctc gggatgttcg cgcacgcgtt cctcgataaa acggttcatg
     4561 cgctgcgcgt cggcttgcgc atcttcactc ggaaaggatt tccaagcagg gtagaaatgc
     4621 aatgtaaccg tattgtctgc ctcgcggacg ggaatggcgg gtatcacttt tgcatttgca
     4681 agcgcggcaa tgcggctcaa tccggtaatc gttgccgtct gaataccgaa gaaatccaca
     4741 aaaaccgaat cgttgcgtcc gaaatcctga tcgggcagat acagaaacgg cgcgctgctt
     4801 ttgcggaact gtttgacgag ggcgcgcagc ccttcggtgc gcccgataag gaagacgttg
     4861 tgatagcggt tgcggccttt caaaatctgt tcgtccaaca tcttgttttt ttgatgagaa
     4921 tacatactga tcagcgggac atcctgatta agcgcgtaca ccgccatctc gaacgcggtg
     4981 aagtgcggat acagaatgat gactttttcc cccgccgcca gcgcgtcgtc caaataatgc
     5041 ttattgcggt agcgcaccag cgatttcaaa cgtccggcag gcgcgtacca atataaaccg
     5101 tattccaaca tcagtttcgc catgtgtttg aaatgctgtt tcaacacggt tttacgcttt
     5161 tcctcactcc attcggaaaa acattttgcc aaattgattt cgccgatacg gcggcgcggt
     5221 ttgaccagaa agtaggcaag cgaacccgtc aggtcggcaa tcttgtgcag cagcgcaaac
     5281 ggcagaaact gcaaaacata cagtacaaaa aatataaatt tcatctcgat acacattttc
     5341 ttttcagacg gcaaaataca aatgccgtct gaaactattg aaacctgccg cgcttgacct
     5401 gcatccccga aggattgagt ttggcggcaa gcccgtggtt gcgtaaggcg tgggtcagcg
     5461 cgacggcaag accgtccgcc gcatccgcct gcggcgttcc cgaaagcccc agcatctgca
     5521 ccaccatatg ctgcacctgt tctttcgccg ccttgccttt gccgactacc gcctgtttga
     5581 cctgcaaggc cgtgtattcc gaaacgggca gcttatggct gaccaatgcc gccaatgccg
     5641 cgcccctagc ctgacccagc atcagcgtcg atgcaggatt gacgttgacg aacacctgtt
     5701 ccactgccgc ctgttgaggc ttgtaaacgg caacgacttc gccgatatgc cgcacaatca
     5761 cggcaatcct gtctgccaga gacgcatcgg caggcgtttt gatgcagccg gaggcaacat
     5821 aaaaatgatc ccgccccctg acatcgatga tgccgaaacc cgttacgcgg ctgcccgggt
     5881 caatgcccaa gatacggacg gttgccgaca tattcacaac aaaccgtgtt gaatcagttt
     5941 tttacgcaag gtattgcggt tcagccccag catcacggag gctttggact ggttgccgcc
     6001 gcattgctcc atcacgcaca ccagcagcgg tttttccacc tgatgcaaga ccatatcgta
     6061 cacgccgcaa ggttcggtac cgttcaggtc tttgaaatat tgttccaaat tttgtctgat
     6121 acattgggaa atatcgggaa gggtatgggg cataatcgcg ctttcaaaag gataatcggg
     6181 tgttcagacg gcattttggc ggtaggcgca cgcccaacgg tcggtttttc cggcaagtct
     6241 ttcaagataa cctgcaagca tgtcgtattg cgccgccgca ctgtccaagc ggttgatttc
     6301 acgacgtgtc tgttcgccgt cgggcatttc gtcgatgtac cagcctatgt gtttgcgtgc
     6361 aatgcgcaca ccggcggtct caccataaaa cgcgtgcatg gcgcggacgt ggttcaaaat
     6421 ggcggcactg cattctgcca aactcaaggc aggcggtaaa acaccgtgtt cggcgtaatg
     6481 tttcaaatcg cggaagaacc acggtctgcc ttgcgcgccg cgccctatca tgatgccgtc
     6541 tgcggcggtt tgtttaagga cggcttgggc tttttgcggc gaagtaatgt cgccgttgac
     6601 ccaaaccggg atgttcagac ggcatttggt ttcggcaatc aggtcgtaag ccgcttcgcc
     6661 tttgtacatt tgcgtgcgcg tgcgtccgtg gacggcaagg gcggcaatgc cgcaatcttc
     6721 ggcgattttg gcgatgacgg gcaggttttt gtggtcgtcg tgccaaccca aacgggtttt
     6781 gagggtaacg ggtacgcccg ccgccttgac caccgcttcc aaaatggcgg caaccagcgg
     6841 ctcgttctgc atcagcgcgc taccggcttg gacattgcag acttttttag cgggacagcc
     6901 catgttgatg tcgataagct gcgccccaag gctgacgttg taacgcgcgg catccgccat
     6961 ctgctgcgga tcgcttccgg caatctgcac ggcaacaatg ccgccttcat cggcaaaatc
     7021 gctgcggtgc aaggtttttc tagtatttct gagcgtcggg tcgctggtca gcatttcgca
     7081 caccgcccaa cctgcgccaa aatctcggca aagtcggcgg aacggtttgt cggtaatgcc
     7141 cgccatcggc gcaagtgcga tggggttgtc gataaaatag ccgccgatgt gcataatggg
     7201 tccgcgtttc aaaaaagtgc gacattgtac attttttaag caggatttcc aatctccgga
     7261 cgcgcccgcg attggggcgg gcaccgtttt atggcataat ccgcacacag atttcctgcc
     7321 ccgccactca caggcgggca
//
LOCUS       DAWFJR010000098         7223 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWFJR010000098 DAWFJR010000000
VERSION     DAWFJR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.098"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(1..75)
                     /locus_tag="WGR04_001787"
                     /pseudo
     CDS             complement(1..75)
                     /locus_tag="WGR04_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(185..442)
                     /locus_tag="WGR04_001788"
     CDS             complement(185..442)
                     /locus_tag="WGR04_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0795294.1"
                     /translation="MASSNVNNEIKDKKLSLWAKRQDGSVKWFCGQPVTRAAVDTADD
                     AVAAAKTSTDNINTKHLPSTCRDDSSVVCIETPPTAFYKNT"
     gene            complement(471..800)
                     /locus_tag="WGR04_001789"
     CDS             complement(471..800)
                     /locus_tag="WGR04_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0795295.1"
                     /translation="MGSLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(878..1096)
                     /locus_tag="WGR04_001790"
     CDS             complement(878..1096)
                     /locus_tag="WGR04_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ0795296.1"
                     /translation="MLKTLTFAALHFSTAFSVTYVLTGSIGISGAVALVEPLINTVVF
                     YFHEKAWNLYEKNKTVKQTQPFQLHRCS"
     gene            1218..2171
                     /locus_tag="WGR04_001791"
     CDS             1218..2171
                     /locus_tag="WGR04_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0795297.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMRVVFAEHKHAHAVREGYVSFEDAVRTADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(2246..4303)
                     /gene="metG"
                     /locus_tag="WGR04_001792"
     CDS             complement(2246..4303)
                     /gene="metG"
                     /locus_tag="WGR04_001792"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ0795298.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFFIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGCLKDVADSALLAKLTAQSEAIAECYESREYAKALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPKVAEN
                     AAKFLNLEAITWANTRETLGKHSINKYEHLMQRVEQKQVDDLIEANKQSIQTTPAPAA
                     EESKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(4419..6257)
                     /gene="glmS"
                     /locus_tag="WGR04_001793"
     CDS             complement(4419..6257)
                     /gene="glmS"
                     /locus_tag="WGR04_001793"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ0795299.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGKLSPVVHTIPVQLLSYHVALARGTDVDKPRNLAKSVTVE"
     gene            6539..7057
                     /locus_tag="WGR04_001794"
     CDS             6539..7057
                     /locus_tag="WGR04_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795300.1"
                     /translation="MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGVIAVKKE
                     KGNYFLNKINVFTGKEESLLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYLDARKALPSNQTYQQHQAAIEQLKRRFEAEFDELEK
                     EIKCNGKPTLLF"
BASE COUNT         1504 a         1698 c         2180 g         1841 t
ORIGIN      
        1 ggcgggccat tcgccgtgat tcaggtaata ctcggtgacg gctgattttt gaccttcgac
       61 caaaaggata gcttcgtcat tcccgcgcag gcgggaatcc agccttgtcg gtacggaaac
      121 ttatcgggaa agcggtttct ttagattttg cgttctggat tcccactttc gtgggaatga
      181 cggtttaggt atttttatag aaagccgtag gtggtgtttc tatgcaaacg acagatgaat
      241 catcgcggca ggttgacggc aggtgcttgg tgttgatgtt gtcggtgctg gtcttggcgg
      301 cggcgacggc gtcgtcggcg gtgtcgacgg cggcgcgcgt aaccggctgt ccgcagaacc
      361 attttaccga accgtcttga cgcttggccc acagggagag ttttttgtct ttgatttcgt
      421 tgtttacgtt gcttgaagcc attatgtcag acggtattgc ccgggcagct ttattcgtac
      481 actttcagca gctcgacttc aaatatcaaa gtggcgtgcg ggggaatcac gccgcccgcg
      541 ccgtgtgcgc cgtagcccat ttccgaaggg atggtcagct tgcgtttgcc gccttccttc
      601 atgccgccga agccttcgtc ccagcctttg atgacttgtc cgacaccgag cgtgatggtc
      661 agcggctggc ggcggtcgag gctggagtcg aatttggttc cgttttccag ccaacctgtg
      721 taatgcacgg taatctcttt gcctttaact gcttcttttc cgaagccttc ctgcaaatct
      781 tcaataatca ggctgcccat atttgtcctt tcgttgcttg ttggtcaaaa cggaaagggt
      841 aacataccgt ccggcgaagt caaatgccgc tcaaacgtca gctgcatcgg tgcagctgaa
      901 acggctgtgt ttgtttgact gttttatttt tttcgtaaag gttccatgct ttttcatgga
      961 aatagaaaac gacggtgttg attaggggtt cgaccagcgc gaccgccccg gatatgccta
     1021 tactgcccgt cagtacatag gttacactga aggcggtgct gaaatgcagt gcggcaaaag
     1081 tcagggtttt aagcatcatc ctctcccgaa ttggacattg acggaggaat gataaaggtt
     1141 atcataaggt tgcgcggttt aaatttgcta tttgttgtta gtgtaaataa atcatttttc
     1201 aaatggggat agaaattatg aatcataaaa agatcgttgt tttggatgcg gatactttgc
     1261 ccggccgggt ttttcatttt gattttccgc acgagcttgc ggtttacggt acgacaggtg
     1321 cggatgaaac ggcggaacgg gtgcgcgacg cacatattgt cattaccaac aaagtgatga
     1381 tttctgccga tattattgcg gctaatccgc agttggagct gattgccgtc agtgcgaccg
     1441 gtgtgaacaa tgtcgatatt ggggcggcga aggcggcggg cgttgcggta tgcaatgtcc
     1501 gcgcctacgg aaacgaatcg gttgcggaac acgcctttat gctgatgatt gcgttaatgc
     1561 ggaatttgcc tgcctatcag cgtgatgttg cggccggttt gtgggaaaag tcgccgtttt
     1621 tctgccatta cggcgcgccg attcgggatt tgaacggcaa aacgctggcg gttttcggac
     1681 gcggcaatat cggacggacg ctggccggat acgcgcaggc gttcggtatg agggtggtgt
     1741 ttgccgaaca caaacacgcg cacgctgtgc gtgaaggcta tgtttccttt gaagatgctg
     1801 tacggactgc cgacgtgttg tcgttgcact gtccgctgaa tgcccaaact gaaaatatga
     1861 taggcgaaaa cgaattgcgg cagatgaagc ccggtgcggt tttgattaat tgcgggcgcg
     1921 gcgggctggt ggatgaaaac gcgctgcttg ccgcactcaa atacgggcag atcggcgggg
     1981 cgggggtcga tgttttgacg gaggagccgc caaggggcgg caatcccctg ctgaatgcac
     2041 gacttcccaa tctgattgtt acgccgcata ccgcgtgggc gagccgcgag gctttggaca
     2101 ggctgttcga tatattgttg gcgaacattc acgcctttgt gaaaggcgag gcgcaaaacc
     2161 gggtagtttg agcttgtcgg gataacgaaa aacaatgccg tctgaacgcc caaagggttc
     2221 agacggcatt ttttgagatt tctacttaac cgactttgtc gcccggttgc gcgcctgcat
     2281 ccacatccaa gagcttcagt ttcccgtctg ccgtggcagc ggagagaatc atgccttcgg
     2341 atacgccgaa ttttgccatt ttgcgcgggg cgaagttggc gacggcgatg accatgcggc
     2401 cgttcaattc ggcaggattt gggtaagacg cggcgatgcc ggagaagatg atgcgttttt
     2461 caaaaccgaa atcgaggtcg aattttaaga gtttggtgct gccttcgacg gcttcgcagt
     2521 tcaatacttt ggcgacgcgc atatcgattt tcataaagtc gtcgaagctc gcctgttcgg
     2581 cgactttttc gtatttgctc tcttcggcgg caggcgcggg ggtcgtctga atgctttgtt
     2641 tgttggcttc gattaaatcg tccacttgtt tttgctccac tcgttgcatt aaatgttcgt
     2701 atttgttgat ggagtgtttg cccaaggttt cgcgtgtatt tgcccaagtg atggcttcca
     2761 aattcaggaa tttggcggca ttttcggcca ctttcggcaa cacgggggcg aggtaggcgg
     2821 tcagcatggt aaaggcgttg atgagttcgc tgcatacttc gtgcaggcgt tcgtcttggc
     2881 cttcttgttt ggcgagttcc caaggtttgt tggcatcaac gtattcgttg acaatgtctg
     2941 ccaaggccat gatgtcgcgc agggctttgg cgtattcgcg gctttcgtag cattcggcaa
     3001 tggcttcgct ttgtgcggtc agttttgcca gcaatgcgct gtcggcaacg tctttcagac
     3061 agccttcaaa gcgtttggcg atgaaacctg aggcgcgggc ggcgatgttg acgtatttgc
     3121 cgacgaggtc gctgttgacg cggctgataa agtcttgcag gttcaaatcg atgtcttcga
     3181 ttttgctgtt gagtttggcg gcgatgtagt agcgcatcca ctcggggttt aaaccttgtt
     3241 ccagatagga tttggcggtg ataaacgtgc cgcgcgattt ggacattttt tgtccgtcta
     3301 cggtcaaaaa gccgtgtgcg tacacgccgg tcggggcgcg gtggccggag aaatgcagca
     3361 tggcaggcca gaacagggcg tggaaataga ggatgtcttt gccgatgaag tggtacatct
     3421 cggtttggct gtcggctttg aagtattcgt caaaatcgac gccgatgcgg tcgcacaggt
     3481 ttttaaacga cgccatgtag ccgacgggcg cgtccagcca gacgtagaag tatttgcccg
     3541 gcgcgtcggg gatttcaaaa ccgaaatacg gcgcgtcgcg ggaaatatcc cagtcggaca
     3601 gggtggtttc ttcaccttcg cccagccatt ctttcatttt gttgagggct tcggcttgca
     3661 gatggggctt gccgtcgtgc gggttgttgc cggaagtcca tgctttgagg aagtcggcgc
     3721 attctcccag tttgaagaag aagtgttcgg attcgcgcaa ttcgggtttg gctccggata
     3781 cggcggaata cgggttgatc agttcggtcg gggaataggt cgtgccgcag acttcgcagt
     3841 tgtcgccgta ttggtcttgg gcgtggcatt tcgggcattc gcctttgacg aagcggtcgg
     3901 gcaggaacat ttgtttttcg gggtcgaaaa gctgctcgat gacgcggctt tcgattttgc
     3961 cgttggcttt cagtgcgcgg taaatgtctt gggaaaactg tttgttttca ggggaatggg
     4021 tgctgtaata attgtcgtag ccgatgaaaa aaccggtaaa gtcggcgagg tgttcttcgc
     4081 gcactttggc aatcatgtct tcgggcgcaa tgccttgttt ttgcgcggca agcatcacgg
     4141 gcgtgccgtg ggtgtcgtcg gcgcagcagt agtggcactc gtggccgcgc agtttttgaa
     4201 agcgcaccca aacgtcggtt tggatgtgtt cgaccatgtg gccgaggtgg atgctgccgt
     4261 tggcataggg cagggcggag gtaacgagga ttttgcgtgt catattgtgc tttgcaaaca
     4321 aagggtaaag gcggattata ccgcaaatca aacggggaaa tgccgtctga agcctgaaaa
     4381 atcaggcttc agacggcatg ttcgcaaaca atccggactt attcgaccgt aacggatttc
     4441 gccaggttgc gcggtttgtc tacatcggtg ccgcgtgcga gggcgacatg gtaggaaagg
     4501 agctgcacgg ggatagtatg cacgacgggg gacagtttgc cgacgtggcg cggtgcgcgg
     4561 ataacgtgca caccttcggt ggcattaaaa ttgctgtcga ggtcggcaaa gacgaaaagt
     4621 tcgccgccgc gcgcgccgac ttcctgcata ttggctttga ctttgtccaa caggctgtcg
     4681 ttgggtgcga tgacgacgac gggcatgttt tcgtccacca aggcaagcgg cccgtgcttc
     4741 agttcgccgg caggataggc ttcggcgtgg atgtaggtga tttccttcag ttttaacgcg
     4801 ccttcgaggg caatcgggta atggatgccg cgccccaaaa acagcgcgct ggttttcttg
     4861 gcaaactgtt gcgcccaggc ggcaatttgc ggttcgaggt ttaaagcgtg ctgcacgctg
     4921 ccgggaagct ggcggagttc ttcggtgtaa cgtgcttcgt cttcttcgga aaccaaaccg
     4981 cgcactttcg ccagcgttac cgccaaaccg aacagcgcaa ccagctgcgt ggtaaatgct
     5041 ttggttgagg cgacgccgat ttccgcaccg gcacgggtat aaagcacgag gctgctttcg
     5101 cgcggcaggg cggattccat cacgttgcag atggagaggc tgtggcggtg tcccaaggat
     5161 tttgcgtatt tcaacgcctc catcgtgtcc agcgtttcgc cggattggga aatggtaatg
     5221 accagttgat ccggatcggc aatcacgctg cggtatcggt attcgctggc gatttcgaca
     5281 tcggctggga ttttggcgat ggattccaac caatatttgg cggtcagtgc ggcgtaatag
     5341 gacgtgccgc aggcaaggat tttgacgctg cggatgcttt caaacacgct tttggcgttt
     5401 ttgccgaagt tttcggggat gaagccgccg tcgaggaaaa cctccgccgt gtctgcaatc
     5461 gcgcgcggct gctcgtggat ttccttttgc ataaagtggc tgtacggtcc cagttccaaa
     5521 gaggcgagcg agagttcgga taccttgact ttgcgttcgg caggcaggcc gtttttatcg
     5581 gtcagccgtt tgatgccgtc tgaagccagc agcgcgatgt cgccgtcttc gaggtacgcc
     5641 acgcggcgcg taaaggcgat gacggcggat acgtccgaag cgataaaggt ttcatcgtcg
     5701 cccaaagcga ccaaaagcgg gcagcccata cgcgccacaa ctaattcatc aggcttgtct
     5761 tgggcaataa ccgcgatggc gtatgcgccg tggaaacgtt tgaccgcttc ttgtaccgct
     5821 tcaaacagcc tgccgccgtt ttgcgcgtat tcgtgattga tgctgtgtgc gatgacttcg
     5881 gtatccgttt gcgattcaaa acggtatccc aaaccttcca aacgtttgcg ttcgctttca
     5941 aagttttcga tgatgccgtt gtgtacgacc gcaatcatac cgccgctgat gtgcgggtgg
     6001 gcgttcggct cagtaacgcc gccgtgtgtc gcccaacgcg tatgtccgat gccgatgccg
     6061 ccgctgatgc ctttttcgcg tgccgcgtcc tccataagct gcacgcgtcc gacgcggcgc
     6121 acacgtttga ttttgccgtc ggtgttgacg gcaatgcctg atgagtcata accccggtat
     6181 tcgaggcgtt taaggccgtc ggtcagaaaa tcgacgacgt tgtgatgggc gcggatggcg
     6241 ccgacgatac cgcacataac tgttccttag tatccggttg aaaaaaaaca ggcgcggacg
     6301 gcttccgtgc cgcaccttcc tcttcggatt ataaaccgcc tcccgcgccg gaaaacagca
     6361 aaatgccgtc tgaaggcttg ggcttgctca aaaaagggag ggatttcccc gcctgaccgg
     6421 ggtgggcgtt cagacggcat tgcctgctgc tggtttatag tttttgcaaa tcaatattga
     6481 caagctgaaa aaaaaaaaca atatactcgc tcggtcttaa tgttaacgga gtatggaaat
     6541 gaaacaaatg cttttagccg tcggcgtggt ggcggtgttg gcgggctgcg gcaaggatgc
     6601 cggcggttac gagggttatt ggcgcgaaaa gtcggacaaa aaagagggcg tgattgccgt
     6661 caaaaaagaa aaaggcaatt acttccttaa taaaatcaac gtatttacag gcaaggagga
     6721 gtctttgctt ttgtctgaaa aagacggcgc gctttcgata aacacgggga taggggaaat
     6781 cccgatcaaa ctttccgacg acgggaaaga gctgtatgtc gaacgcaggc agtatgtcaa
     6841 aaccgatgcg gcgatgaagg acaaaatcat cgcccatcag aaaaagtgcg gacaaacggc
     6901 acaggcatac ctcgacgcgc gaaaggcgtt gccgtcaaac caaacgtatc agcagcatca
     6961 ggcggcgatc gagcagttga aacggcggtt tgaagccgag ttcgacgaat tggaaaaaga
     7021 aatcaaatgc aacggcaaac cgacattgtt gttttagcgt aggacaaccg ggaggatgcc
     7081 gccgtccgaa tcggatgtgt ggttttcgta ccggtacggg cgggcaggaa tgtccgcttt
     7141 ttttgttcgg acgcgtttga atatccgtcc gattccgtcc gtttgcaaag ggtatttttg
     7201 ttcgggcgga aattatagtg gat
//
LOCUS       DAWFJR010000099         7075 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWFJR010000099 DAWFJR010000000
VERSION     DAWFJR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7075
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.099"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            123..1973
                     /locus_tag="WGR04_001795"
     CDS             123..1973
                     /locus_tag="WGR04_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0795301.1"
                     /translation="MYLPSMKHSLPLLATLVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LGARKQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDADANSVLRMAEDGAIKRALVLFQNSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMANAAYYASLFGAPHIPLKQRMGIVPAR"
     gene            2262..2474
                     /locus_tag="WGR04_001796"
     CDS             2262..2474
                     /locus_tag="WGR04_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ0795302.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            2606..3052
                     /locus_tag="WGR04_001797"
     CDS             2606..3052
                     /locus_tag="WGR04_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ0795303.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(3130..3372)
                     /locus_tag="WGR04_001798"
     CDS             complement(3130..3372)
                     /locus_tag="WGR04_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795304.1"
                     /translation="MPSERNRVSDGILSDGAQTESVYRQNPFRCIETVLPLPIRYWDV
                     IASALECNYGAANQAIERQRQRAAAVAVHERVAKFL"
     gene            complement(3377..3769)
                     /locus_tag="WGR04_001799"
     CDS             complement(3377..3769)
                     /locus_tag="WGR04_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ0795305.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(3841..4446)
                     /locus_tag="WGR04_001800"
     CDS             complement(3841..4446)
                     /locus_tag="WGR04_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ0795306.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            complement(4548..6224)
                     /locus_tag="WGR04_001801"
     CDS             complement(4548..6224)
                     /locus_tag="WGR04_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ0795307.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGHADFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPTVHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADASQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
     gene            complement(6370..6999)
                     /locus_tag="WGR04_001802"
     CDS             complement(6370..6999)
                     /locus_tag="WGR04_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ0795308.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNSANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVSGQEKIL"
BASE COUNT         1833 a         1948 c         1893 g         1401 t
ORIGIN      
        1 atgtcggaaa cattccaaag gcggtgcagt ttcggcatat aattcgggca aacgcctgtt
       61 cagacggcat tttgtctttt ccaaccctga ccgttcaggg ttccgattct taaggaaatc
      121 cgatgtacct accctctatg aagcattccc tgccgctgct ggcgacccta gtgcttgccg
      181 cgtgttcttc gacaaacaca ctgccagccg gcaagacccc ggcagacaat atagaaactg
      241 ccgacctttc ggcaagcgtt cccacccgcc ctgccgaacc ggaaggaaaa acgctggcag
      301 attacggcgg ctacccgtcc gcactggatg cagtgaaaca gaaaaacgat gccgccgtcg
      361 ccgcctattt ggaaaacgcc ggcgacagtg cgatggcgga aaatgtccgc aacgagtggc
      421 tgaagtcttt gggcgcacgc aaacagtgga cgctgtttgc acaggaatat gccaaactcg
      481 aaccggcagg gcgcgcccaa gaagtcgaat gctacgccga ttcgagccgc aacgactata
      541 cgcgtgccgc cgaactggtc aaaaatacgg gcaaactgcc ttcgggctgc accaaactgt
      601 tggaacaggc agccgcatcc ggcttgttgg acggcaacga cgcctggagg cgcgtgcgcg
      661 gactgctggc cggccgccaa accacagacg cacgcaacct tgccgccgca ttgggcagcc
      721 cgtttgacgg cggtacacaa ggttcgcgcg aatatgccct gttgaacgtc atcggcaaag
      781 aagcacgcaa atcgccgaat gccgccgccc tgctgtccga aatggaaagc ggtttaagcc
      841 tcgaacaacg cagtttcgcg tggggcgtat tggggcatta tcagtcgcaa aacctcaatg
      901 tgcctgccgc cttggactat tacggcaagg ttgccgaccg ccgccaactg accgacgacc
      961 aaatcgagtg gtacgcccgc gccgccttgc gcgcccgacg ttgggacgag ctggcctccg
     1021 ttatctcgca tatgcccgaa aaactgcaaa aaagcccgac ctggctctac tggctggcac
     1081 gcagccgcgc cgcaacgggc aacacgcaag aggcggaaaa actttacaaa caggcggcag
     1141 cgacgggcag gaatttttat gcggtgctgg caggggaaga gttggggcgc aaaatcgata
     1201 cgcgcaacaa tgttcccgat gccgacgcaa acagcgtcct ccgtatggcg gaagacggcg
     1261 cgattaagcg tgcgctggta ctgttccaaa acagccggac cgccggcgat gcgaaaatgc
     1321 gccgtcaggc tcaggcggaa tggcgttttg ccacacgcgg gttcgacgaa gacaagctgc
     1381 tgaccgccgc acaaaccgcg ttcgaccacg gtttttacga tatggcggtc aacagcgcgg
     1441 aacgcaccga ccgcaaactc aactacacct tgcgctatat ttcgccgttt aaagacacgg
     1501 taatccgcca cgcgcaaaat gttaatgtcg atccggcttg ggtttacggc ctgattcgtc
     1561 aggaaagccg cttcgttata ggcgcgcaat cccgcgtagg cgcgcagggg ctgatgcagg
     1621 ttatgcctgc caccgcgcgc gaaatcgccg gcaaaatcgg tatggatgcc gcacaacttt
     1681 acaccgccga cggcaatatc cgtatgggga cgtggtatat ggcggacacc aaacgccgcc
     1741 tgcaaaacaa cgaagtcctc gccaccgcag gctataacgc cggtcccggc agggcgcgcc
     1801 gatggcaggc ggacacgccc ctcgaaggcg cggtatatgc cgaaaccatc ccgttttccg
     1861 aaacgcgcga ctatgtcaaa aaagtgatgg ccaatgccgc ctactacgcc tccctcttcg
     1921 gcgcgccgca catcccgctc aaacagcgta tgggcattgt ccccgcccgc tgacgtaccg
     1981 atgccgtctg aaacccgccc ggtctttcag acggcatttt tatcccgaac ggcattgacg
     2041 gcgaaccata aatataagac aatccgaaaa ttgtttttcc tgctttttca agcagcttga
     2101 cacggcacaa gccgacccgt taggaggtga tgtttccgtc acggcgcgta tcccgccgcc
     2161 gcaaggcaca gcgatacggt aaaccttcaa caccgtctgc cctacccttt ccaccgatat
     2221 gatgggcaga tgaaacaacc gaatttatta aaggaaataa aatgcctgca atccgcgtaa
     2281 aagagaatga accatttgaa gtcgctatgc gccgtttcaa acgcgccgta gaaaaaaccg
     2341 gcctgctgac cgagctgcgc gcccgcgaag cctacgaaaa accgactacc gaacgcaaac
     2401 gcaaaaaagc ggcagccgta aaacgcctgc aaaaacgcct gcgcagccaa caactgccgc
     2461 ccaaaatgta ctaaacgttc aagtacagat tacaggtcag ccctgtgata tgaggacaca
     2521 ccgcaagacc tgctctgcgg tgtgttttgc ttttcagacg gcatcgaaac ccgccgtttc
     2581 catccgacat cccagcgagg acatcatgag cctgaaaatc cgccttaccg aagacatgaa
     2641 aaccgcgatg cgcgccaaag accaagtttc cctcggcacc atccgcctca tcaacgccgc
     2701 cgtcaaacag tttgaagtgg acgaacgcac cgaagccgac gatgccaaaa tcaccgccat
     2761 cctgaccaaa atggtcaaac agcgaaaaga cagcgcgaaa atctacactg aagccggccg
     2821 tcaggatttg gcagacaaag aaaacgccga aatcgaggta ctgcaccgct accttcccca
     2881 aatgctctcc gccggggaaa tccgtactgc ggtcgatgcc gccgttgccg aaaccggcgc
     2941 ggcaggtatg gcggatatgg gcaaagtgat gggcgtattg aaaacccgcc tcgccggcaa
     3001 agccgatatg ggcgaagtca acaaaatcct gaaagccgtg ctgaccgcct gattgcccga
     3061 atatcggaca aaatgccgtc tgaagcccgt atcgcaggtt cagacggcat tttcatggtt
     3121 tgatgatatt tataaaaatt ttgcaacacg ctcatgcaca gcaactgccg ctgctcgttg
     3181 gcgttgccgc tctatcgctt ggttagctgc accataattg cactctaatg ctgatgcaat
     3241 aacgtcccaa tatcgaatcg gcaggggcaa cacggtttcg atacaccgaa acgggttttg
     3301 ccgataaacg gattccgttt gcgccccatc ggacaaaatg ccgtctgaaa cacgattccg
     3361 ttcagacggc atagatttat ttgaccaatt tcaagccttt tttggcgggc cggggcgcgg
     3421 tttcggcaga ggtgttttca ggcggcgtgt cggggcgata cgcttccaat tcaaacccca
     3481 taccttcgcc ggtctcccgt gcgaaaaggc tgaggacgtg tccgacaggt atccatatgt
     3541 cgtgcgcctg tccgccgaag cgggcggaaa agctgatcca atcgttgtcg atttgaaggt
     3601 tttgcgtggc ggtcgcgccg atgttgagca taatttcgtt gtcgcggacg tactgcatgg
     3661 ggacacgcgt gtgttcgttg acccagacaa gaatgtgcgg cgtgaggctg ttatcgctgc
     3721 accattcgca aagtgcgcgg aggatgtagg gtttggtgga agtgggcata atgggttccg
     3781 tgtggtgtgc ctgaatgtga aaatgcctac aaaacggcgg gttttgcaag cattccgggc
     3841 ttatttgcgc atggcttttt cggcgggtgt cagtgcttcg ataaaggctt cgcgctggaa
     3901 gatgcgctcg gcgtatttga gcagcggcgc ggcacttttg cccagtttga catcgtagtg
     3961 gtcgagccgc cacagcagcg gagcaagggc gacatcaatc atagaaaaat cttcgccgag
     4021 gatgtatttg cttttgctga acgaaggggc aagcatggtc agaccgttgc cgatggcttc
     4081 gcgcgctttt gcctgttcct tgttggtggc ggcggggttt tctaacactt ggacgtggtt
     4141 gaacaattct ttttccatac ggtacagcac cagccggccc cggccgcgca taacgggatc
     4201 gccgggcata agctgcggat gagggaagcg ttcgtcgatg tattcgttga tgatgttgga
     4261 ctcgtgcagc accaaatcgc gctcgaccag cacgggaact tggttatacg gattcatgac
     4321 ggcgaggtct tcgggtttgt tgtaaatatc gacgtctttg atttcaaaat ccataccttt
     4381 ttcgtacaaa acgaagcggc agcggtggct gaaggggcag gtaatgccgg aatagagggt
     4441 catcataata attgtcgctc ctgtgtgatg cctgcaaaac ggctgattta taaaggttta
     4501 atcgcgcaat tatacgcgat ttccggcact taatccagaa attcggctca atctgttgtt
     4561 tttaatatat tttccccgat tttccgtatc agtgcgaact tactgtcttt gttgcgcggg
     4621 cgcgcaccct gccagaccgt ctgccagcct tgcgacgcat ccgcattttg gggcaagcgg
     4681 acgatacggt agcggcattg tacatcgccg acgcggtgcg gcaatgtgcc gtactgcgtc
     4741 caaacaatcc gcgtgtgcag gtcgccgccg cctatgtcga tacactcgat gccgtctgaa
     4801 agctcccgtt ttaattccgg ggaaagcgat gcctccatac tccggacgac cggcgcgtgg
     4861 cttttcgccg cgtccagcca cggcaggaac agcgtcatca gcaaagccca ggtcagggta
     4921 acgcctgccg cccagttggt aaccgcctgc ctgccgcgta tgtttttgcg ggtaatcgcc
     4981 cacagccaca agggtgtgaa cagtacggca accgccatcg gaatgggatc gatatcagga
     5041 acataatacg ggctgaaata ggcggcgcgt tcggcaagct tggcgggcca gccgtaattc
     5101 atggcgaaaa agcccgtcca caggaacacg gcaaacagtc cgaacgccat aatgccgaac
     5161 cagttgacaa acgccgccgc gccgcgcctc aggctgtcca gttgcgccgc gccgaacagg
     5221 gcaagcggcg gaagcagcca gacgaggtta tcctgaaaac gctgcggatt gacggcaagc
     5281 agcaccaaaa cggcaagcat ccagacgacg cccaaaatcc cccagtcggt cgaaaacagg
     5341 cgcgtgcggc aaaccgtcca aaccgccagc ggcagcgcgg gcaatgcaaa ccaaagcagg
     5401 tttttcagat agtaaaacaa actgaatgcc gtctgaacgt gccgcacgcc gccgaacgta
     5461 ccgaaaacgt gatcgtcgag ccattgcgcg aacagcgcgg gctgcgtttt tgccaagagc
     5521 agcgggtaaa cggtcataag cggcagggca aaggcaagtg aggcgactgc cgtcaacatc
     5581 aaacgcctgc tttgccacgg acggaaaaac atcagtacgg gcaagggcag catcagggca
     5641 aatgctgccg gataagctgc tgccaacgac atcagcgtcc agcccgtacc gagcagaaaa
     5701 gaggcggcaa tcacgcgccg gcgagccaaa gaataaccgt gcagcaccag tccggcggcg
     5761 gcaaaggcgg cggcagcggg gttgagaaag tgtacggtcg gaatcagccc gatacagccg
     5821 atgagaatca ggacgacgct gcgcccgtgg tgtctgccca aaaagttgaa gccagcaaag
     5881 ccgcaggaag tcagcccgac aacggcgaaa aacacgcctg caaagcgtgc ggcatcatac
     5941 gggtcggctg cccacggcga cagcaaatgt ttgaacgcgg cggcaaccca aagatacacg
     6001 ggtggtatgc cgaaatcggc atgcccgaac agatgggcaa ccaagggggt ggggctgcct
     6061 gccagtgctt cgacggcggt atagacggca ggttcggcag gattccacaa atcgtgggaa
     6121 aacacgccgg gccacaacca ggcaaacgcc atcaacagca gcagccacgg cttttcgtgg
     6181 gttttggcgg gcgggcgggc atcgggcgga gtgtaggtca gcataagcgt gagaaaaaat
     6241 ggacggattg ccaagtgtag caaatattcg cacaaaggtc gtgcagagac tgcttcagac
     6301 ggcatcagac acaaaaagac cggcaacaaa aaagactgca catggcagtc tttgcagata
     6361 ctatcttttt cataatattt tttcctggcc cgacacagtc catagcatat caaaactgtt
     6421 gttttcaatc aggatatata cccccaatcc taaataaaca acagcaacaa accatctgct
     6481 atatttttcc aaagtttctc cgacagaagg gacttgtgcc aatttttggg cagaaaaaac
     6541 caagagataa atcatgacta gaaaggtaag taaagccact atcaaattcg ctgaatttaa
     6601 ggtagtaaaa tatgggacaa agacaccaat attgtcagca ccacaacttg caaaagtaat
     6661 catagcgact agaaaaatca ggtttttatt atctttgcgc aaaccctctt tggcaatagc
     6721 ctctccatca gaatctccta acagcaaaac tttgatgcct aggaaaattg gaatcaagcc
     6781 gagcaaacct aaaatctctt tactaggaat ataatctaag acaaatgcaa aaagcaaact
     6841 tagcaatatc agactaacag agcctagaaa ttgtcctaaa tagatgttaa tgatgtcttt
     6901 tctgcttttt cttttggcaa aaaataacat taggataata agtaagtcta cggctgtccc
     6961 agaatacagg attattgaag taacgacatt ttgaatcata aaacatctca ttcaaatata
     7021 tttttaaatg tattcaaaca ttaaaccttg tagatgtcaa cttcaacccc gtcaa
//
LOCUS       DAWFJR010000100         7053 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWFJR010000100 DAWFJR010000000
VERSION     DAWFJR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.100"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            102..1007
                     /gene="glyQ"
                     /locus_tag="WGR04_001803"
     CDS             102..1007
                     /gene="glyQ"
                     /locus_tag="WGR04_001803"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0795309.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1084..1494
                     /locus_tag="WGR04_001804"
     CDS             1084..1494
                     /locus_tag="WGR04_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEZ0795310.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQTLIFGVQGSLHQAE
                     DDAEAFGRFMDELIAYLVVNGFEMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     DREDVATLYATPLVDGFYGDDGWENYSETYKQAD"
     gene            1515..3578
                     /locus_tag="WGR04_001805"
     CDS             1515..3578
                     /locus_tag="WGR04_001805"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ0795311.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAASVAEGLEKAQLIAGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGELLEDIINAAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSDGEITIENADSYAEQMREQGKVVA
                     SFAERKAAIQTALEGQARRLNASVAADETLLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAVEQHYQPRFAGDKLPESKIA
                     TAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGEVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3595..4641
                     /locus_tag="WGR04_001806"
     CDS             3595..4641
                     /locus_tag="WGR04_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ0795312.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLEILIVDDGSTDGTLAIAKDFQKRDSRIKILAQAQNSGLIPSLNIGLDELAKSG
                     MGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHRHG
                     KIWKKPTRPEDIADFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSK
                     LGRLAYYPEALVKYRLHANQVSSKYSIRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQIKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTLPAGAWLDFAADGRMRRLFTL
                     RQYFGILHRLLKNR"
     gene            4683..5510
                     /locus_tag="WGR04_001807"
     CDS             4683..5510
                     /locus_tag="WGR04_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0795313.1"
                     /translation="MQNHVISLASAAERRAHITDTFGVRGIPFQFFDALMPSERLEQV
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYITVFEDDVLLGEGAEKFLA
                     EDAWLQERFDPDSAFIVRLETMFMHVLTSPSGVADYGGRAFPLLESEHWGTAGYIISK
                     KAIRFFLDRFAALPPEGLHPVDLMMFSDFFDREGVPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQWRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            5522..5617
                     /locus_tag="WGR04_001808"
     CDS             5522..5617
                     /locus_tag="WGR04_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ0795314.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILRRLLKNR"
     gene            5659..6501
                     /locus_tag="WGR04_001809"
     CDS             5659..6501
                     /locus_tag="WGR04_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0795315.1"
                     /translation="MQNHVISLASAAERRAHIAATFGARGIPFQFFDALMPSEELNRM
                     MAELVPGLAKQHLLSEVEKACFMSHAVLWKQALDEGLPYVAVFEDDVLLGKDAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQGRRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT         1865 a         1863 c         1810 g         1515 t
ORIGIN      
        1 atgtatgccg tgttttcaaa tcagtctgtc ttcgagataa acctatccaa acagcaatgg
       61 aaacagtaaa ataaaccctt tattttatcg aatcataagc catgcttacc ttccaacaaa
      121 tcatcttcaa actgcaaaca ttctgggcag acaaaggctg caccgtcatc caacccttcg
      181 acatggaagt cggtgcaggt acttcccatc ccgccacctg cctgcgtgcg ctcgggcccg
      241 agccttggtt tgccgcctac gtccaaccca gccgccgccc caaagacggc cgctacggcg
      301 acaaccccaa ccgcctgcaa cactattacc aattccaagt cgccctcaaa cccgcccccg
      361 ccaatatcca agacctttat ctcgactccc tgcgcgaatt gggcatcgac cccaaagtgc
      421 acgacatccg ctttgtcgaa gacgactggg aaaaccccac cctcggtgcg tggggtttgg
      481 gctgggaagt ctggctcaac ggtatggaag taacccagtt cacctatttc caacaagtcg
      541 gcggcatcga ctgcaccccc gtactcggcg aaatcaccta cggcatcgaa cgcttggcga
      601 tgtacctgca aggcgtggaa aacgtctatg acctcgtttg ggcaaaaacg ctggacggta
      661 atacagtaac ttacggcgac gtgtaccacc aaaacgaagt cgaacaatcc acctacaact
      721 tcgaatacag cgatgccgat tggctgctgc gccagttcaa cgactacgaa gcgcaagcca
      781 aacgcctgct tgccgaagaa aacgccgccc tcgccttgcc tgcctacgag ctggttctca
      841 aagcggggca tacgttcaat cttttagacg cacgcggcgc gatttccgta accgagcggg
      901 caacttatat tggacgtatt cgtgcattga gccgcgccgt ggcgcaaaaa tatgttgaaa
      961 gccgcgaaaa actgggcttc cctttaatga aggcaaatgc tgcctaaggg ggcgacgatt
     1021 ttttacgttc gggcggcacg ataaggcagc cgtccgtaaa ccattatata ttttaggaaa
     1081 atcatgcctg tctatttccg caccccaagc aggaaacgcc tgcacaaaat gaaccgccgc
     1141 cagcataaaa agctgcattt gggcgagttt caaacattga ttttcggcgt tcaaggcagt
     1201 ttgcaccaag ctgaggacga tgccgaagcg ttcggccgct ttatggacga gctgattgcg
     1261 tatcttgtgg taaacggctt tgaaatggcg ggcgggggcg attgcggcga tttttcgtgc
     1321 ctgtttcaaa aatccggtgc ccgaagcttg ggagaaaccg agaaaaaagc cattatctct
     1381 tggctggcag accgcgaaga tgttgccact ttgtacgcca ccccgctggt tgacggtttt
     1441 tatggggatg acggctggga aaattacagc gaaacctata aacaggcaga ttaaccaaat
     1501 tcaaagaaag ccgaatgatg acccaaaccc ttttaatcga actccttacc gaagaacttc
     1561 ccccaaaagc cctgaataat ttaggcaacc atttcgccgc ttccgttgcc gaaggcttgg
     1621 aaaaagcgca actgatagcc ggcgcggcag aatttaccgc ctacgcttct ccgcgccgtt
     1681 tggcggttca agtcaaaaac gtcaaagccg tgcaagccga tcagaaaatc gtgaaaaaag
     1741 gccctgccgt ggcgaatgcc gtaaaagacg gtgcgccgac caaggctttg gaaggtttcg
     1801 cacgcggtgc aggggcgaaa atcgaagact tgaccatcgt ccacgacggc aagcaggacg
     1861 tgtacgccta cgaatacgtc caaaccggca aaccgttggg cgaacttttg gaagacatta
     1921 tcaatgccgc agtgaaaaaa ctgccgattc caaaagtaat gcgttggggc agcagcacat
     1981 ttaccttcgt gcgccccgtc cacgggctga ttgtgctgca cggcggcgac atcgtaaacg
     2041 tcagcgtttt gggtctgcaa agcagcaatc aaaccttggg acaccgcttc ctctccgacg
     2101 gagaaatcac cattgaaaac gccgacagct acgccgaaca aatgcgcgag caaggcaaag
     2161 tcgtcgcttc gtttgccgag cgcaaagccg cgattcagac ggcattggaa gggcaggcac
     2221 gccgtctgaa cgcctccgtt gccgccgatg agaccctgtt ggacgaagta accgcgctgg
     2281 tcgaatggcc tgttgttttg gaagccggtt ttgaggaaca tttcctcgcc gtgccgcagg
     2341 aatgcttgat tttaacgatg cagcaaaacc aaaaatactt tccgctgctc gaccaaaacg
     2401 gcaagctgat gaaccgcttc ctgctggttt ccaacctgca aaccgaagac ccgtcgcaca
     2461 tcatccgagg caacgaacgc gttttgcgcg cgcgcctgtc tgatgccgag ttcttctaca
     2521 aacaagacca aaaagcgact ttggaaagcc gcctgccgaa attgtccagc gtggtgtacc
     2581 acaacaaaat cggctcgcaa gccgaacgca tcgaacgcct gcaaagcatt gccgcccaca
     2641 tcgccaaagc attgggcgcg gatgccgccg ctgccgaacg cgccgcgcgt cttgccaaag
     2701 ccgacttggt aaccgaaatg gtcggcgagt ttcccgaact gcaaggcacg atgggcaaat
     2761 actatgcccg cttggacggc gaaaccgaag aaatcgccga agccgtcgag caacattacc
     2821 aaccgcgttt tgccggcgac aagctgcccg aaagcaaaat tgccactgcc gttgcactgg
     2881 ccgacaagct ggaaaccctg gtcggcattt ggggcatcgg tctgattccg accggcgaca
     2941 aagaccccta cgccctgcgc cgcgctgcct tgggtatttt gcgtatgctg atgcagtatg
     3001 gtttggacgt gaacgaactg attcagacgg cattcgacag cttccccaaa ggtttgctca
     3061 acgaaaaaac gccgtctgaa accgccgact ttatgcaggc gcgtttggcc gtgttgctgc
     3121 aaaacgatta tccgcaagac atcgttgccg ccgtattggc gaaacagccg cgccgtttgg
     3181 acgatttgac cgccaaactg caagccgttg ccgcgttcaa acagctgccc gaagccgccg
     3241 cgctcgccgc cgccaacaaa cgcgtgcaaa acctgctgaa aaaagccgat gccgagttgg
     3301 gcgaagtcaa tgaaagcctg ctgcaacagg acgaagaaaa agccctcttt gccgccgcgc
     3361 aaggcttgca gccgaaaatc gccgccgccg tcgccgaagg caatttccaa accgccttgt
     3421 ccgaactggc ttccgtcaaa ccgcaagtcg atgcattctt tgacggcgtg atggtaatgg
     3481 cggaagatgc cgccgtaaaa caaaaccgcc tgaacctgct gaaccgcttg gcagagcaaa
     3541 tgaacgcggt agccgacatc gcgcttttgg gcgagtaacc gttgtacagt ccaaatgccg
     3601 tctgaagcct tcagacggca tcgtgcctat cgggagaata aattgcagcc tttagtcagc
     3661 gtattgattt gcgcctacaa cgtagaaaaa tatttcgccc aatcattagc cgccgtcgtg
     3721 aatcagactt ggcgcaactt ggagattttg attgtcgatg acggctcgac agacggtacg
     3781 cttgccattg ccaaggattt tcaaaagcgg gacagccgta tcaaaatcct tgcacaagct
     3841 caaaattccg gcctgattcc ctctttaaac atcgggctgg acgaattggc aaagtcagga
     3901 atgggggaat atattgcacg caccgatgcc gacgatattg ccgcccccga ctggattgag
     3961 aaaatcgtgg gcgagatgga aaaagaccgc agcatcatcg cgatgggcgc gtggctggaa
     4021 gttttgtcgg aagaaaagga cggcaaccgg ctggcgcggc atcacaggca cggcaaaatt
     4081 tggaaaaagc cgacccgacc cgaagatatt gccgactttt tccctttcgg caaccccata
     4141 cacaacaaca cgatgattat gaggcgcagc gtcattgacg gcggtttgcg ttacaacacc
     4201 gagcgggatt gggcggaaga ttaccaattt tggtacgatg tcagcaaatt gggcaggctg
     4261 gcttattatc ccgaagcctt ggtcaaatac cgccttcacg ccaatcaggt ttcatccaaa
     4321 tacagcatcc gccaacacga aatcgcgcaa ggcatccaaa aaaccgccag aaacgatttt
     4381 ttgcagtcta tgggttttaa aacccggttc gacagccttg aataccgcca aataaaagca
     4441 gtagcgtatg aattgctgga gaaacatttg ccggaagaag attttgaacg cgcccgccgg
     4501 tttttgtacc aatgcttcaa acggacggac acgctgcccg ccggcgcgtg gctggatttt
     4561 gcggcagacg gcaggatgcg gcggctgttt accttgaggc aatacttcgg cattttgcac
     4621 cgattgctga aaaaccgttg aaaaacgccg ctttatccaa cagacaaaaa acaggataaa
     4681 ttatgcaaaa ccacgttatc agcttagctt ccgccgcaga acgcagggcg cacattaccg
     4741 ataccttcgg cgtgcgcggc atcccgtttc agtttttcga cgcactgatg ccgtctgaaa
     4801 ggctggaaca ggtaatggcg gaactcgtac ccggtttgtc ggcgcacccc tatttgagcg
     4861 gagtggaaaa agcctgcttt atgagccacg ccgtattgtg gaagcaggca ttggacgaag
     4921 gtctgccgta tatcaccgta tttgaggacg acgttttact cggcgaaggc gcggagaaat
     4981 tccttgccga agacgcttgg ctgcaagaac gctttgaccc cgattccgcc tttatcgtcc
     5041 gcttggaaac gatgtttatg cacgtcctga cctcgccctc cggcgtggcg gactacggcg
     5101 ggcgtgcctt tccgctgttg gaaagcgaac actgggggac ggcgggctat atcatttcca
     5161 aaaaagcgat acggtttttc ctggacaggt ttgccgccct gccgcccgaa gggctgcacc
     5221 ccgtcgatct gatgatgttc agcgattttt tcgacaggga aggagtgccg gtttgccagc
     5281 tcaatcccgc cttgtgcgcc caagagctgc attatgccaa gtttcacgac caaaacagcg
     5341 cattgggcag cctgatcgaa cacgaccgcc tcctgaaccg caaacagcaa tggcgcgatt
     5401 cccccgccaa cacattcaaa cgccgcctga tccgcgcctt gaccaaaatc agcagggaaa
     5461 gggaaaaacg ccggcaaagg cgcgaacagt tcattgtgcc tttccaataa aaggaggaga
     5521 gatggacatc gtatttgcag cagacggcag gatgcggcgg ctgtttacct tgaggcaata
     5581 cttcggcatt ttgcgccgat tgctgaaaaa ccgttgaaaa acgccgcttt atccaacaga
     5641 caaaaaacag gataaattat gcaaaaccat gttatcagct tagcttccgc cgcagaacgc
     5701 agggcgcaca ttgccgcaac cttcggcgcg cgcggcatcc cgtttcagtt tttcgacgca
     5761 ctgatgccgt ctgaagaact gaatcggatg atggcggaat tggtgccggg attggcaaaa
     5821 cagcacctgt tgagcgaagt ggaaaaagcc tgctttatga gccacgccgt attgtggaaa
     5881 caggcattgg atgaaggtct gccctatgtt gccgtatttg aggatgatgt cctgcttggc
     5941 aaagacgcag aaaagttcct tgccgaagat acttggttgg aagagcgttt tgataaggat
     6001 tctgccttta tcgttcgttt ggaaacgatg tttgcgaaag ttattgtcag accggataaa
     6061 gtcctgaatt atgagaaccg gtcatttcct ttgctggaga gcgaacattg ggggacggct
     6121 ggctatatca tttcgcgtga ggcgatgcgg tttttcttgg aaaggtttgc cgttttgccg
     6181 gcagagtgga ttaaagcggt agattggatg atgtttactt atttctttga taaggagggg
     6241 atgcctgttt atcaggttaa tcccgcctta tgtacccaag aattgcatta tgccaagttc
     6301 ctcagtaaaa atagtatgtt gggtagcgat ttggaaaaag atagggaaca aggaagaaga
     6361 caccgccgtt cgttgaaggt gatgtttgac ttgaagcgtg ctttgggtaa attcggtagg
     6421 gaaaagaaga aaagaatgga gcgtcaaagg caggcggagc ttgagaaagc ttacggcagg
     6481 cgggtcatat cgttcaaata gtttgtgtaa aatatagcgg attaaaatta gaaatggata
     6541 cactgtcatt cccgcgaaag tgggaatcca gtccgttgag tttcagtcat ttccgataaa
     6601 tcaccgcaac gttaaatttc tagattcccg cttttgcggg aatgatggcg ggggggattt
     6661 ctgtttttcc gacaaattcc taaaacttaa aatttcatca tttctgcgaa gacagaaaac
     6721 caaaaacaga aacctgaaaa cagaaacctg aaaacagaaa cctgaaaaca gaaacctaaa
     6781 attcgtcatt cccgcgcagg cgggaatcca gtccgttgag gttcagtcat ttccgataaa
     6841 tcaccgcaac attaaatttc cagattcccg cttttgcggg aatgacggcg ggggggattt
     6901 ctgtttttcc gacaaattcc taaaacttaa aattccgtta ttcccgcgag aacagaaaac
     6961 caaaaacaga aacctaaaat ctcgtcattc ccgcaaaaac agaaaaccaa aaaacagcaa
     7021 cctaaaattc gtcattcccg cgcaggcggg aat
//
LOCUS       DAWFJR010000101         6691 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWFJR010000101 DAWFJR010000000
VERSION     DAWFJR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6691
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.101"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            964..1200
                     /locus_tag="WGR04_001810"
     CDS             964..1200
                     /locus_tag="WGR04_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ0795316.1"
                     /translation="MKELHTSELVEVSGGKFHISAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGISKKW"
     gene            2422..3687
                     /locus_tag="WGR04_001811"
     CDS             2422..3687
                     /locus_tag="WGR04_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ0795317.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVENGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLVEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKREQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            3690..5777
                     /locus_tag="WGR04_001812"
     CDS             3690..5777
                     /locus_tag="WGR04_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEZ0795318.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHVIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAVFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
     gene            complement(5859..6161)
                     /locus_tag="WGR04_001813"
     CDS             complement(5859..6161)
                     /locus_tag="WGR04_001813"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795319.1"
                     /translation="MKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAGFNGDI
                     GEKLHRCAVMEWAENETVKLAQKWEQEQKKQQIQQKKETEKSPKHKASRDDWEMER"
     gene            6228..>6691
                     /locus_tag="WGR04_001814"
     CDS             6228..>6691
                     /locus_tag="WGR04_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0795320.1"
                     /translation="MRKSRLSRYKQNKLVELFVAGVTARTAAELVGVNKNTAAYYFHR
                     LRLLIYQNSPHLEMFDGEVEADESYFGGQRKGKRGRGAAGKVAVFGLLKRNGKVYTVT
                     VPNTQTATLFPIIREQVKPDSIVYTDCYRSYDVLDVSEFSHFSFAETSFSYQS"
BASE COUNT         2193 a         1159 c         1202 g         2137 t
ORIGIN      
        1 aaaattccat gtttgtgtac tgaaatgctt caaaacacca aaccaagttt cgttttctaa
       61 aatacgaaac cattactgct gcctaaattt ttttggattg ctaaattatg gcagtatgat
      121 tttggatttt aaattgaaag gcaagaaaaa tgtcaaaaaa tgatgtagtt aaagtaattg
      181 gtatattccc cctattgtcc gaacaataga gcagacttcc cggcaggtca aattgtttta
      241 aaacacggcg aacacaaaaa ggttaatcac ggctttggtg tgattcacat tttggcagaa
      301 cacaaagcag atttaacgcg gtttggatta agccacgatg aaacaggtgt aaccctttta
      361 tgtggaaatg atttttcaat caggcgcaaa aatttttagt gaatttagtg atgtaagggg
      421 caatcatcgt cctatgattg tctattctcg gattgggaca gtcgttttag aacgcaaaga
      481 agttgagggg gaaagtgtgt attcagttgt taccgcgttt gggcgaacaa gccctcgtgg
      541 tacacaaatt ggtacaatgc cataaaaaga caaaagcacc caataattca ggtgctttgt
      601 gtagtagccg cagcctctcc ttagcttgat agggtcggat accaatctga cgattgagct
      661 actgaaattg gttagcgaca accaacctgc ccacatcaga acgcccgttc gctggtttgt
      721 acgtcctgaa aaagcttttg cattaagtta atcataatgg gaaatttaaa ttttttcaat
      781 gcttacttaa acaaaagccc cactccacca ttaggagttt ctttttcagt atacaagtaa
      841 atatttttaa aatattgatt taatttaaaa taaaatactt gcaaaaaaaa agtattaaat
      901 taaacttaag aaaggttaat tctgatttac atttccaacc atacttcttt acaggagaaa
      961 atcatgaaag agttacacac ctctgaatta gttgaagtgt caggtggcaa attccatatc
     1021 tctgcacagg gtggcggcaa cctaggtaaa aaagatatgg ttgctgttgg taaaattggt
     1081 gcttcctatt cccctaacaa tagtggagta gagttttctg ttagcaagca atttggatat
     1141 gtacaaggtc ttggtgtaca gttttcgaaa cctacttttg gtattagtaa aaaatggtaa
     1201 gattttttgt tttatccttt ctgacattaa taaatctatg ctcattaagc gcatgcaata
     1261 gccactttac aggaaatatc aatccattag gtactcacaa taaagttgct aatcccaatt
     1321 gtgccaatag tgccaatagt catatcagac aacccagtag gaaaaactat gatccaactg
     1381 aatatagtgc ttggttacag tatatgcatg attgcaaata atgagtaacg atgaaaattt
     1441 acttttttct caaccacact taacaaaggt gaatattatg caagttttga ctttgaatga
     1501 aatttaacaa gtttctggtg ctgcttgtaa ctggcgtgat ttctcaaaaa ataccattgg
     1561 tagtgcatta ggtggagcag ctggtggggc aattgttggt tcatttgcag gtggtattgg
     1621 tgctattcca ggtgcgaaat tcggagctat tggtggtgca atcactggtg ctgtacaata
     1681 tggaagcact tgttggtggt aatattcctt aataaaacta gggtattttg atattttcta
     1741 ttcaaaatac cctagttttt cataagaact taaatacaaa aaggaacaaa taatgaaaaa
     1801 atatagtgat tattttaaat atttaatctt ttttttgatt ttactcccaa caaattatct
     1861 cgtatctcat tatgtggtac aaacctcaat gagtatgtta agcattttaa gttcttctat
     1921 aataacaact tgcttttttt tgtttttaca aatttatccc aatcaaagaa acataaataa
     1981 gtacacatgt ctaacaatca ctcatttttc agaccagaag tctttgtagc tcaacggaac
     2041 aagtggacag gaccagtagg ctgggttggc gcaattggag ctggtatttt ctctgctgct
     2101 ggcggataca atatcggtcg tggcatgatg aagccataag ataattacat cattaaggaa
     2161 aaggtaattt cagttacagc aatatgtatt gaagttacct ttttctattt agattgaaca
     2221 attttgaaag agaaaaatta tgaatactga aaccatttac gccactgtct tttgcatttt
     2281 agctgcaacc atttctggat tattggttaa atttaatgta attaaaatag aaacatcaat
     2341 caatagcaaa ttcatgttat taggcataag tatttaatta ttggtgtttt tctatccatt
     2401 ttttttaaga aataataata aatgtcccac ttattccgaa aagaagtctt tgtagcccaa
     2461 caaaataagt ggacaggtca ggttatcttg acccgtccat tctctttttt atttctgact
     2521 ttttgcgctt ttctcattgc tctgtgtatc attatctttt tgatttttgg tagctatacc
     2581 aataaaacaa ccgttgaagg tcaattactt ccaactatgg gggtggttcg tgtttactct
     2641 tccgatatcg gcacgattac gcataaattt gttgaaaatg gtaactttgt caaagctggc
     2701 gaaccattgt tcaaactttc cacatcgcgt tttggcgaaa aaggaaacgt acaagccaaa
     2761 ttggcagcag aagccaacct taaaaaaact ttggcattac aagaattgga acgtttaaag
     2821 cgcattcatc aaaatgagca aaaaaatgtt cataacaaca ttcatcgttt aaacaatcaa
     2881 ttagagaata ttaaacagca aattacaggg caaaatcgtc aaattcgttt agtggaaaaa
     2941 acccttaaca agaacaagtt tttagccagt caaggcgcag tatcccaaca agataagatg
     3001 accgccgaaa gccatttatt ggaacaacgc tcacgtttgg agagcctaaa acgtgaacaa
     3061 aataatgcaa tcagggaact tgatgaacag aaaatcacat taagcagcct gcctgaacgc
     3121 cataaaaccg aattgagcca actcaaccgt gcgattacgg aaatgaacca agaaattttg
     3181 gattttgatt tgaaatccga acaaaccata cgagctagta aatcaggtta tatttcaaca
     3241 attaatgttg atatagggca acaagttgaa ccgtctaaat tgctgttaag cattgtccct
     3301 gaacaaactg aattggtcgc caatctttac atacccagta aagctgttgg ttttattaaa
     3361 ccgaaagata aagttgtttt acgttaccaa gcgtaccctt accaaaaatt tggacatgcc
     3421 acaggagaaa ttatttcagt tgccagaact gctctcggta aacaagagct atcaggttta
     3481 ggtatcattt tcactaaccc aaccttatta aatgaacctg cctatcttgt gaaagttaaa
     3541 ttggaaaaac aaacgattaa agcatacgga gaaaacaagc cgcttcaaat tggcatgatt
     3601 ttagaagcag atattctcca tgaacgaaaa aaattgtacg aatgggtact tgacccactt
     3661 tacagcattt caggaaaaat caattaaaaa tggattattt atcaagactg tcctttggat
     3721 ttaacaaaaa gctacctgtc attctgcaaa cagaagttgc tgaatgtggt ttagcatgcc
     3781 tgacatccat cttgtcctat tatggctttc acactgattt aagaacgtta cgccaaaaat
     3841 acaccctgtc attaaagggc gcaaatcttg cagacatcat gagatttggc aatgaaatga
     3901 atttaacgcc acgagctttg cgtttagagt tagatgagct gtcaaattta caactaccct
     3961 gcattctcca ttggaactta aaccattttg ttgtactttg ttccatttcc aaagacagta
     4021 tcgtcattat ggaccctgct gtcggtatgc gaaaaatcaa aatggacgaa gtttcacaaa
     4081 aattcacagg gattgcccta gaattattcc ccaataccca ttttgaagag aaaaaagaaa
     4141 caaagaaaat caaaatatta tctctattaa gggggatttc aggcttaaaa cgttctttaa
     4201 ttcaaatgct tatattagct atttctttgg aagtctttgc attggttagt ccattcttta
     4261 tgcaatgggt aatagaccat gtcattgtaa ctgctgataa aaatttatta ctgaccctta
     4321 ctttgggatt tggtttactg gctatcctgc aacagttaat tagcctgtta caagcatggg
     4381 taggtatgca cctatctaca actcttaatt tacaatggaa agccaatata tttaaaaggt
     4441 tacttgactt acctaatgac tatttcagta aacgacattt aggagatgtg atttcaagat
     4501 ttggttcaat agatcatatc caagaaacac taacttctac tttttttgtt ttagttttaa
     4561 atagcttaat ggctgttttt actttcgtgt taatgacaat ttacagcact caattatcgc
     4621 tgattgttct tttaacactt gttttgtaca tactaattcg ttggcttgca tattacccat
     4681 taagaaatgc aacagaagaa aatattgttc atgaagccaa acaaaactca tatttcatgg
     4741 aaaccattcg tggtatccaa tcagttaaat tatttgataa acattatcaa agacatggca
     4801 cttggatgag cctatttgtg aatacagtca ataccaagct gacaacagat aaactctctg
     4861 ctttatttga attttcaaat aaactgttgt ttagcatgga aaatattatc ataatttatc
     4921 ttggtgcaag cgcaatttta gatggttcat ttacagtcgg tgttctgatg gcttttttgg
     4981 cttataaagg gcaatttgaa agcagaacag cttctctcgt tgaccaatac atccaaatca
     5041 aaatgttagg gcttcatgct gaacgtttgg ctgacattac tttaaatgaa acagaaactg
     5101 aaattattaa gtataatcat atacctaaat tagataatga acaactggtt cttaaagttg
     5161 aaaacgtctc attcagatat gctgataatg agccatatct ttttgaaaac attaatttgg
     5221 aatttaaaga taatgaagca gttgttttaa caggacaatc tggtcggggg aagtccactt
     5281 tgttaaacat tttaacaggt agcctaaaac ctgaaactgg tacagttagt attaatgggc
     5341 atgatatata tcaagtttct ccatccttta ttaggggatt gagcgggatt gttcgccaag
     5401 atgatgtcct ttttgcaggt tctattgggg aaaatatttc attttttgat gaaagcccaa
     5461 atatggagct cattgaacaa tgtgcaaaaa tggcacaaat acatgacgat atacttaaaa
     5521 tgccaatggg ctatgagacc ttgattggcg atatgggaaa tatcttatca ggtggacaaa
     5581 agcagagagt tatcttggct cgtgcattgt ataaacgacc caaaattcta tttttagacg
     5641 aagcaagtag ccatttagat gtagaaaatg aacaaaaaat taaccataac ctaaaaagtc
     5701 ttggtattat gaaaataatg gttgcacacc gccaagaaac aattcaatcg gcagataaaa
     5761 ttctgaattt aggttgaaca gaacaagact tcatttttct ttaacaaaaa gtgaagtctt
     5821 ttttcaaata atttaataga atacatgaaa atagcggttt aacgttccat ttcccaatca
     5881 tcacgactgg ctttgtgttt tggcgatttt tcagtttcct ttttctgttg aatttgttgt
     5941 tttttctgct cttgttccca tttttgggct aatttcacgg tctcattttc agcccattcc
     6001 atcacggcac aacgatgtag cttttctccg atatcgccat taaagccagc tccacgaact
     6061 tcaccataaa ttcttgaata tttttgatta tattcaattt cttttccatt ttctttaaag
     6121 gatttctccc acttttcaca aacttcatca aaatctttca aagggatatt ttttaagggg
     6181 ctgtcctaga taactaggga aattcaaatt aagttagaat tatccctatg agaaaaagtc
     6241 gtctaagccg gtataaacaa aataaactcg ttgagctatt tgtcgcaggt gtaactgcaa
     6301 gaacggcagc agagttagta ggcgttaata aaaataccgc agcctattat tttcatcgtt
     6361 tacgattact tatttatcaa aacagtccgc atttggaaat gtttgatggc gaagtagaag
     6421 cagatgaaag ttattttggc ggacaacgca aaggcaaacg cggtcgcggc gctgccggta
     6481 aagtcgccgt attcggtctt ttgaagcgaa atggtaaggt ttatacggtt acagtaccga
     6541 atactcaaac cgctacttta tttcctatta tccgtgaaca agtgaaacct gacagcattg
     6601 tttatacgga ttgttatcgt agctatgatg tattagatgt gagcgaattt agccatttta
     6661 gcttcgctga aacttcgttt tcgtatcaat c
//
LOCUS       DAWFJR010000102         6645 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWFJR010000102 DAWFJR010000000
VERSION     DAWFJR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.102"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..294
                     /locus_tag="WGR04_001815"
     CDS             <1..294
                     /locus_tag="WGR04_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0795321.1"
                     /translation="YDSAENRQHLKEHQLLDGIMRKACRNRPLTETQTKRNRYLSKTR
                     YVVEQSFGTLHRKFRYVRAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            412..786
                     /locus_tag="WGR04_001816"
     CDS             412..786
                     /locus_tag="WGR04_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795322.1"
                     /translation="MSAVGRERNFAKVSYRELVERQLAVRHADLELGLSRAREQEPFV
                     IHVSDLLDKAGIEYAVRMDKDFQTTFNLEYPNTNYDTFKRVVRQMISAYYCVCNDGDE
                     LEISSNRPDGYAVRIVFGDVPV"
     gene            798..1064
                     /locus_tag="WGR04_001817"
     CDS             798..1064
                     /locus_tag="WGR04_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795323.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAQADRKNIAASLERIENKLEKVNEKLDRKADKS"
     gene            1061..1258
                     /locus_tag="WGR04_001818"
     CDS             1061..1258
                     /locus_tag="WGR04_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795324.1"
                     /translation="MKDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTALTF
                     GALGGGVITVGWELLKAKMGL"
     gene            1261..1767
                     /locus_tag="WGR04_001819"
     CDS             1261..1767
                     /locus_tag="WGR04_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ0795325.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQATARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     AEVEKKFG"
     gene            complement(1764..2276)
                     /locus_tag="WGR04_001820"
     CDS             complement(1764..2276)
                     /locus_tag="WGR04_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795326.1"
                     /translation="MTGFISNAFIGLKEAGSLLKGFNQLKTEAEVAQKTIELNQIIAG
                     IQTDLFEAQSSYTAAISRIDELEKELVAVKNWSTEKQRYQLYELALGSFVYRLKAEMA
                     NSEPIHDICPQCYEQGVKSILQFNGYEGAFHKYSCSRCQTIILGERLKSDVEVFSVGG
                     SRYGRLTDGY"
     gene            2288..3910
                     /gene="terL"
                     /locus_tag="WGR04_001821"
     CDS             2288..3910
                     /gene="terL"
                     /locus_tag="WGR04_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ0795327.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDASPKAITARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTVLDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWRGVKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYQANKDEMERGAVTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIVIGGYQRSMG
                     RLYVVEAQIKKRLPDLIIEDVICLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            3910..5478
                     /locus_tag="WGR04_001822"
     CDS             3910..5478
                     /locus_tag="WGR04_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ0795328.1"
                     /translation="MAKKDNKTKIQKPEAALQTDAAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     SGTMATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPNADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPINRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            5465..6418
                     /locus_tag="WGR04_001823"
                     /pseudo
     CDS             5465..6418
                     /locus_tag="WGR04_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            6479..>6645
                     /locus_tag="WGR04_001824"
     CDS             6479..>6645
                     /locus_tag="WGR04_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0795329.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWHPIEHYLNRQRTR
                     YLRDHRGRPAYP"
BASE COUNT         1735 a         1646 c         1806 g         1458 t
ORIGIN      
        1 tatgacagtg cggaaaaccg gcaacatctg aaagaacatc agttgttgga cggcattatg
       61 cgcaaagcct gccgcaaccg tccgctgacg gaaacgcaaa ccaaacgcaa ccggtatttg
      121 tcgaagaccc gttatgtggt tgaacagagc ttcggtacgc tgcaccgtaa gttccgctat
      181 gttcgggcag cctattttgg actgattaaa gtgagtgcgc aaagccacct gaaggcgatg
      241 tgtttgaacc tgttgaaagc cgccaacagg ctaagtgcgc ccgctgccgc ctaaaaggcg
      301 gtccggatgc ctgattatcg ggtatccggg caggattaag ggggtatttg ggtaaaatta
      361 ggcggtattt ggggcgaaaa cagctgaaaa cagccgaaaa cctgtgtttg gatttcggct
      421 gtcgggaggg aaaggaattt tgcaaaggtc tcctaccgtg aattagttga acgtcagttg
      481 gctgtgcgcc atgccgattt ggaattgggc ttaagccgcg cacgcgaaca agagccgttt
      541 gtcattcatg tttccgacct gttggataag gcaggcattg agtacgcggt acgcatggat
      601 aaggattttc agacgacctt taaccttgaa tatccaaata caaactacga cacctttaag
      661 cgtgtagtta ggcagatgat ttcggcgtat tactgcgttt gtaatgatgg agatgaactc
      721 gaaatttcca gcaatcgccc tgacggttac gccgtccgta tcgtattcgg cgatgtgccg
      781 gtttaaaggg gttttaaatg gactttgaat ttggtttcag aaccctgtgg ccgattgcga
      841 cggcggcatt ttggttttgg gtcaacggca tttcaggccg tctgaaagag gcggacaagc
      901 gtatcgatga ccttaaagag gagctgcacg cggtcaagct ctcgtaccac accaagcagg
      961 atgcccaagc cgaccgcaaa aatatcgcag cgtctttgga gcgcatcgaa aacaagttgg
     1021 aaaaagtaaa cgaaaaactg gacaggaaag cggacaaatc atgaaagacc cgattttgga
     1081 agccttggcg cgtattgaaa acaaaactga tcagacgctg aaaaatcaga aggaaatgca
     1141 ggcggaaatt gcacaaatcc gccaagacac gaaacgcacg gcccttacat tcggcgcact
     1201 gggcggcggc gtgattacgg tcggctggga attgcttaaa gcgaaaatgg gactgtaatt
     1261 atggctcacc cgcaagaaat ccgtgaaaag ttacgtcggc tctatgtgag cggcgagcaa
     1321 actttggaaa cggcggcctt gatgtgcgaa atcccgcagg ccactgcgcg tgcgtggaaa
     1381 cgtgcggata aggaaaaagg cgacgactgg gataagatgc gcgccgccta cactttggcc
     1441 ggcggcggta ttgaggattt gagccgcgcg atgttggccg gttttatggt gcagtacaac
     1501 agcacgatga cgatgctgca ggattcgagc accgaagatt tgccgccgtc cgaccgcgcc
     1561 aagctgttgg ccagcctggc cgatgcgttt accaaaaccg tatcagccaa tgcgcgtgtg
     1621 atgccggaaa cgtcaaaact ggcgacggct ttggaattga ttgagttctt gatggcattt
     1681 gtgcaagaaa aacaccccaa acatttgcct gcctttgtcg aggtattgga gccgtttggg
     1741 gcggaagtgg agaagaagtt tggttaatag ccgtctgtca atctcccata acgtgatcct
     1801 cctactgaaa atacttcaac atctgacttt aaacgctctc ccaatattat ggtttggcaa
     1861 cgggaacagg aatatttatg aaaagcacct tcatacccat tgaattgaag aatcgatttg
     1921 acaccctgct cgtaacattg gggacaaata tcatgaattg gctcgctgtt cgccatctcc
     1981 gctttcaatc gatagacgaa agaaccaaga gcaagttcat acaattggta gcgttgcttc
     2041 tctgtagacc aattttttac tgccaccagt tctttttcca actcatctat acggctaatt
     2101 gccgccgtat aagaagattg ggcttcaaat aaatcagttt gaataccagc aatgatttga
     2161 ttaagttcga tggttttttg cgctacctct gcctctgttt tgagctggtt aaaccctttt
     2221 aaaagtgatc ctgcttcttt taggcctata aatgcgttag aaatgaatcc agtcattata
     2281 aatactcatg aaaacaaaag aattcctcaa atcccttgcc gaactggccg ccagcctgcg
     2341 ccaagtcatc gaagcggaag tggacggctt cgatgcgtcg ccaaaggcta ttactgcacg
     2401 ccgtgccaag gtatttgacc cggtaggcgg ttacgaatat ttcgtgaata cctacttccc
     2461 ccattatatc cgctcgcctg aaaaatccga actgcatgcg tttttgttca gccgtctgcc
     2521 ggagattatc cgctccccca aaggggaaaa tgaggcggta ggtgcgccgc gcggcgaagg
     2581 taaatcgacg caggttaccc agttgtttac gctgtggtgt attgtgaccg gccaaaaaca
     2641 ttatgcggtc atcgtgatgg acagtatcga ccaggcgtat ccaatgctcg aagccattaa
     2701 ggcagaactt gagtttaacc cgcgcctgaa aaccgacttt ccggaagtat gcgggcaagg
     2761 ccgtgtatgg caggccggta cgattgtgac ggccaatgac gttaaagttc aagtggccgg
     2821 tagcggtaaa aagctgcgcg gtttgcgtca cggcccttac cgtcctgact taactgtttt
     2881 ggacgatatt gagaatgacg aacaagtccg caaccccgaa cagcgcgaca agctcaatgc
     2941 gtggctgact aagactgtat tgcctttggg cggtgtcggt cagaaatacg atgtgattta
     3001 tatcggcacg attttgcatt acgacagcgt acttaaccgc actttgaata acccgttttg
     3061 gcggggtgtt aagtttaagg cgatgaaacg ctggcccgac cgcatggact tgtgggacag
     3121 atgggaggaa cttttccgaa acgacggcga gacggtggcc gaggcgtttt atcaggcaaa
     3181 caaagacgag atggagcgcg gcgcagtcac ttcttgggcg gcgcgtggcg tactcgcgct
     3241 gatgaaaatc cgcgcccgtg acggtcatgc gacatttgac agcgaatatc aaaacgaccc
     3301 agtcagtggc gaagatgcgc cgtttgccaa gtcgatgaag ttttggaacg acctgccgtc
     3361 cgatttggtg tatttcggtg cgctcgaccc gtcgctgggt aaagcggggg cgagccgtga
     3421 cccgtcggca atcgtcatcg gcggctatca gcggtcaatg ggcaggcttt atgtcgtgga
     3481 ggcgcaaatc aaaaaacgtc tgcccgacct gattatcgaa gacgttatct gcctgcaccg
     3541 tcaatatcgt tgcaaactgt ggtttgttga gacggtgcag tttcaggagt ttttaaaaga
     3601 cgagctggtc aagcgcagcg cggcgcgtgg aatacctgtc ccggcgcggg cggtcaagcc
     3661 ggtatcggac aagctcttgc ggattgagac tttgcagcct cacatggcga acggtttgat
     3721 tctgttgaat gagagccaac aaacgctgat acagcagttc cgccattttc caaaggctga
     3781 tcatgatgat ggtcctgatg ccgtgcatat gctctggtcg ggggcggtgg cgaattgtgt
     3841 gccgatagaa tggcaaagcc ctaccgataa cgattttgat gacgagataa aaagtaaatg
     3901 gagccgataa tggcaaaaaa ggacaataaa actaaaatcc aaaagcccga ggctgcattg
     3961 cagacggatg cggctcaaat tacggcgacc ggtcgggtta tcgccgagca tccgtccaat
     4021 tttattacgc cgcaaaagat gcgggccctc ttcgaggacg cagaaagcgg cgacatccgc
     4081 gcccaacacg agcttttcgc ggacattgag gagcgcgaca gcgacatcgc ggcaaatatg
     4141 ggtacgcgca aacgcgcgct gctgacgctc aactggcgcg tcgccccgcc gcgaaatgcg
     4201 acgcccgaag aagaaaagct gtccgaccaa gcctacgaaa tgatggacag cctgcctacc
     4261 ctcgaagacc tgattatgga tttgatggac gcggtagggc acggattttc tgcgttggag
     4321 gtcgagtggg tattttcaga cggcctttac ctaccccgaa actttatcca ccgcccgcaa
     4381 agctggttca aatgggacaa agacaacggg ctgctgctgc gtacccgcga aaatccggaa
     4441 ggcgaagcgt tgtggccgct gggctgggtc gttcataccc aaaaatcgcg cagcgtccag
     4501 caggcgcgca acgggctttt ccgcacgctt tcctggctgt atatgttcaa acactacgcc
     4561 gtccacgatt ttgccgagtt tttggagctg tacggcatgc ccatccgtat cggcaaatac
     4621 ggcgcgggcg caaccaaaga ggaaaaaaac accctgcttc gagcggtggc ggaaatcggt
     4681 cacaacgcgg caggcatcat gccagaaggt atggaaatcg agctgcacaa cgctgcaagc
     4741 ggtacgatgg caaccagcaa tccgtttttg cagatggccg actggtgcga aaaatcggcg
     4801 gcgcggctga ttttggggca aacgctaacc agcggtgcgg acggaaaatc cagcaccaac
     4861 gcgctgggca atatccacaa cgaggtacgc cgcgatttgc tggtgtcgga cgcaaaacag
     4921 gtggcgcaaa ccatcacaag ccaaatcatc ggaccgttcc tgcaaatcaa ctatcccaat
     4981 gccgacccaa accgcgtgcc gaaatttgaa tttgacacgc gcgagccgaa agacatcgcg
     5041 gtctttgccg acgctatccc gaaactggtg gatgtcggcg tacaaatccc cgaaagctgg
     5101 gtgcgcgaca aactggtcat tccagatgtg caggagggtg aggctgtgtt ggtgcggcag
     5161 gtaccggaca atccgataaa cagaactgca ttggcggctt tatccgccca caccgtacca
     5221 tctaaggcta cgggcaggca tcaggaaata ttggacggcg cgttggatga cgcgctggtt
     5281 gagcccgatt tcaattctca gctcaacccg atggtgcgtc aggcggttgc cgcacttaat
     5341 gcttgcaaca gctacgagga ggcagatgcc gcactgaatg cgctttatcc gaatttggac
     5401 aacgcgaaac tgcgtaccta tatgcagcag gccttgttta tcagcgatat tttgggacaa
     5461 gaccatgccc gcgcctgatt tgggatttgc cttaagtctg ccgccaaaaa aggcaatcga
     5521 gtggctggaa agtaaaaagg ttacggcgga gagctaccgc aatctgacag cctccgaaat
     5581 tgccaaagtc tatacgattg cccgcatgac cgacttggat atgctcaacg acatcaaaac
     5641 ttcgatggtt gaatcggcaa aaagtggaca gtcgtttgac gattggcgaa aaggtatctt
     5701 gaatctgctc agcaacaagg gctggctgca tccgaacggg cataacggta aggatatcat
     5761 cgacccagcc accggcgagg tattcggttc gccgcggagg ttggagacga tttaccgtac
     5821 caacatgcaa actgcctaca acgccggtca atatcaagga tatatggcaa atattgatgc
     5881 acgaccttat tggatgtatg acgcggtagg cgacagccgc acccgtccgg cgcattcggc
     5941 aatagacggg ctggtgtacc gctacgacga cccgttttgg gcaacgtttt acccgcccaa
     6001 cggctacaac tgccgctgct cggtcatcgc tctgtcggag cgggatgtgg aacgccaggg
     6061 gcggattgtc gggcaaagca cggcggacaa tctggtcgag acccataaaa tctacaacaa
     6121 aaaaggcgat acttatctga cccttgccta taaagcaccg gatggcagtc tgtacacgac
     6181 cgatcgagga tttgattaca acgccggacg aatgaactac cgccccgatt tagacaagta
     6241 cgaccgtgcg ttggcgcatc aatttgccaa agcggaaatg ggtggtgcgg attttaaaac
     6301 cagctttaaa cagcttgaaa aagagtttta tgaagtcaag caacgtttga atattgatgg
     6361 caagcccgat aaagagcaga aaatcaaaat ccgaaacgcg ctatcaagac agcttgagac
     6421 ctttgcaaaa ttccccaaaa tcccctaaat tcccaccatg acatttagga gatttctcat
     6481 gagcaccttc ttccaacaaa ccgcccaagc catgattgcc aaacacatcg accgcttccc
     6541 actattgaag ttggaccagg tgattgattg gcacccgatc gaacactacc tgaaccgtca
     6601 aagaacccgt taccttagag accaccgcgg ccgtcccgcc taccc
//
LOCUS       DAWFJR010000103         6521 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWFJR010000103 DAWFJR010000000
VERSION     DAWFJR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.103"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(371..2299)
                     /gene="dnaK"
                     /locus_tag="WGR04_001825"
     CDS             complement(371..2299)
                     /gene="dnaK"
                     /locus_tag="WGR04_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ0795330.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDHIIAEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKAEALGTASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2507..2779)
                     /locus_tag="WGR04_001826"
     CDS             complement(2507..2779)
                     /locus_tag="WGR04_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795331.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEKFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2965..3651
                     /locus_tag="WGR04_001827"
     CDS             2965..3651
                     /locus_tag="WGR04_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0795332.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3783..4121
                     /gene="erpA"
                     /locus_tag="WGR04_001828"
     CDS             3783..4121
                     /gene="erpA"
                     /locus_tag="WGR04_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ0795333.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            4138..4284
                     /locus_tag="WGR04_001829"
     CDS             4138..4284
                     /locus_tag="WGR04_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795334.1"
                     /translation="MPSERSDGIFTFGKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4281..4532
                     /locus_tag="WGR04_001830"
                     /pseudo
     CDS             4281..4532
                     /locus_tag="WGR04_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            4686..4898
                     /locus_tag="WGR04_001831"
     CDS             4686..4898
                     /locus_tag="WGR04_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease III domain protein"
                     /protein_id="HEZ0795335.1"
                     /translation="MELETVVPPEEGEGEGEGADGEMPEYLELHGGREDDISAPSQPE
                     PPTRNIKPLLFGLLIAFLGYLLGKIF"
     gene            4933..6444
                     /gene="ubiB"
                     /locus_tag="WGR04_001832"
     CDS             4933..6444
                     /gene="ubiB"
                     /locus_tag="WGR04_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ0795336.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGRRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
BASE COUNT         1492 a         1451 c         1732 g         1846 t
ORIGIN      
        1 aaccgaacag actagattcc cgcctgcgcg ggaatgacgg ctgcagatgc ccaacggtct
       61 ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acagatagta
      121 cggaaccgat tcacttggtg cttcagcacc ttagagaatc gttctctttg agctaaggcg
      181 aggcaacgct gtactggttt aaatttaatc cactatataa aaaatttcca gaggaccgat
      241 acaacagttg gaacttgggt ttgggaatat tacggtagat gaacttggaa cctctgttat
      301 gctatggtct tttatctcaa ttgaaaaaag cgcgaatcaa acggttcgcg cttttttcag
      361 acggcatcaa ttattttttg tcgtctttta cttcttcaaa gtcggcatct acgacatcat
      421 cgtctttctt tgcagaagca ttggcttgtt cgctttcgcc tgcttgggct tcagcttgtg
      481 cttgagcgta aaccatttcg cccagttttt ggctggctgt gcccagcgcc tcggctttgg
      541 catcgatggc ggctttgtcg tcgcctttca cggcttcttc ggcttctttc agcgcggctt
      601 cgattttttc tttttcggca gcgtcgagtt tgtcgccgta gtccgccaaa gattttttca
      661 cggagtgaat cagggcttcg gcttggttgc gggaagcgac caattcagtc agctttttat
      721 cttcctcagc attggcttcg gcatctttca ccatgcgttc gatttcttct tcgctcaaac
      781 ctgaagaacc ttggatggta atgttggctg ctttaccggt gcctttgtct ttggcggaaa
      841 cgtgcaggat gccgttggcg tcgatgtcga aggttacttc gatttgcggc ataccgcgcg
      901 gtgcaggtgc gatgtcgccc aagttgaact gacccaaaga tttgttggca gaagcgcgtt
      961 cgcgttcgcc ttgcagtacg tggatggtta ctgcgctttg gttgtcttcg gcggtagaga
     1021 acacttgcga cgctttagtc gggatagttg tgttcttttg aatcagcttg gtcatcacgc
     1081 cgcccatggt ttcgataccc aaggacagag gggttacgtc cagcagcaat acgtcgctgc
     1141 ggccgccgct taacacttcg ccttgaatcg ctgcgcctac ggcgacagct tcgtcagggt
     1201 tcacgtcttt gcgcggctct ttgccgaaga agtctttaac ggcttcttgt actttcggca
     1261 tacggctttg accaccgacc aaaatcacgt cgtcgatgtc gccagtgctc agaccggcat
     1321 ctttcaatgc ggtgcggcaa ggctcgatag agcgggcaat caggtcttca accaggcttt
     1381 cgaatttggc gcgggtaatt ttcatcgcca agtgtttcgg gccggttgcg tccatggtga
     1441 tgtacggcag gttaatttcg gtttgctggc cgctggacaa ttcgattttg gctttttcgg
     1501 cagcttcttt caggcgttgt agagccatca cgtcttgttt caaatcaatg ccttgttctt
     1561 ttttgaactc ggcgatgatg tggtcgatga ggcgttggtc gaagtcttca ccgcccaaga
     1621 aggtatcgcc gttggttgcc aatacttcga attgtttgtc gccgtcgagg ttggcgattt
     1681 cgatgatgga aatatcaaaa gtaccgccgc ccaagtcata tacggctact ttgcggtctt
     1741 tgttgtcgcc tttgtccata ccgaatgcca aagcagctgc agtcggctcg ttgatgatgc
     1801 gtttcacgtc caaaccggcg atacggcctg cgtctttggt agcttgacgt tggctgtcgt
     1861 tgaagtaggc agggacggta atcacggctt cggttacttt ttcgcccaag taagcttcgg
     1921 cggcttcttt cattttacgc aggacttctg cggaaatttg aggaggagac agctctttgc
     1981 cttgtgcttt tacccatgcg tcgccgttgt tggctttgat gatttcgaaa ggcatagatt
     2041 cgatgtcgcg ttggacttct ttgtcttcaa atttgtggcc gatcaggcgt ttggcggcgt
     2101 aaatggtgtt tttagcgttg gttacggctt ggcgtttggc aggcgcaccg acgaggattt
     2161 cgccgccgtc caaataagcg ataacggacg gcgtggtgcg tgcgccttct gcgttttcga
     2221 tcactttggt ttgaccgttt tcggaaatgg ccaaacaaga gttggttgta cctaagtcga
     2281 taccgattac ttttgccatg tggataatcc tatttgattt tgcttatttt gagaaatatg
     2341 ttggaacatt ttgtcccgat gggctgtaaa tagggcgggc ggcgggctgt ttcaagctac
     2401 agcatggcta taagtatata actttatgaa tatattggtt ttatatttga tttaatacat
     2461 ttggctccaa tgcatttaag cataatattt caaatggcag gcaggtttat tcatagacga
     2521 tgccggcgag catttcctgt tcgttcaagt tgccgtactc tttttcccag tcgtgtgaag
     2581 actcgatgat gtcgcattct ttggaaaggg agacttgttc tgcatccata tctttggcgt
     2641 tcagtatgtt gaatttttcg cacagggatg cggataaagt gatgtcgggc tgtttggctt
     2701 cagaacggtt ttcttggaag gcaaagcaga atgcggtaaa tgccgcagca tagataagat
     2761 atttgccggt tttcttcatt ttctatcctt tttctgtcaa ctcaggatta aacctatgga
     2821 aaatcttaaa aattatgtat tgagtaagaa aaatcataat ttaaatttag tttatcatag
     2881 ttgttccgtt tttgggatag ctaaggtaaa atatatttca tgtttacttt agatgattga
     2941 atgaagggga gcggaaggat atttatggaa acctttaaag acagactggt ttttttatgg
     3001 aaaagcgaag cgaagcaggc aaaaatcgca tccgatattg aaatgacgat tgcgggcttc
     3061 agcaggatat ggaatgaagg cggtctgccg aagtctgaaa cattaaaaaa aatcaagcag
     3121 ttgaaggggt gtagtatcga ttggctgctg accggggagg gtaatccgtt tccggatgaa
     3181 gccccaaaaa aatcccttgc ttacgatact ttgggcaatg aagtcgatac ggacgagttt
     3241 gtcttcgtgc cgagatatga tattcgggcg gctgcgggat acgggcagtt tgtcgatcat
     3301 gaggaaccgg tatttacaat ggcgttcaga cggcattgga ttgagaatta tgttacccgc
     3361 gatacgaaaa acctgtctgt aatttccgtc aagggggatt cgatggaggg ggttttgaat
     3421 gacggcgatt cgattttggt caatcatggt gaaaatacgc cgagggacgg tctgtatgtg
     3481 ttgcggatta atgaaaatct gctggttaaa cgtttacaga ttgtaccggg cgggattatc
     3541 aatgtgattt ctgcaaacga ggcttatcct gcttttgaaa tcaatttgaa cgatttgacc
     3601 gatgatgtgg agattatcgg gcgtgtcgag tggttcggca ggacgatttg agtttggggc
     3661 ttgaaattgc aggcggtcaa acttatctat tggaacaatt cctttttcaa aggcgaagcc
     3721 tgcttgcctt taaagggggt ttgagagaga atgcagaaaa tattatatta aggaatcaca
     3781 ccatgtcgga tgaaagccct attattttta ctgacagctg ctgtgccaaa gttgccgatt
     3841 tgattgccga agaaaacaat cccgatttga aattgcgggt ttttgtcaat ggcggcggct
     3901 gttcgggttt ccagtacgga tttacttttg acgaaatcaa aaacgacgac gattttgaaa
     3961 ttgagaaaaa cggtttggtc tttttggtcg atccgatgag ctatcaatat ctggtcggtg
     4021 cggaaatcga ctatacggaa agtttgcagg gttcgcaatt cgtcatccgc aatccgaatg
     4081 cggaaacaac ctgcggttgc ggatcgtcgt tttccgtatg accgcttggt ttgtgtgatg
     4141 ccgtctgaac gttcagacgg catttttact tttggaaaat atattatcgg gatgaattca
     4201 catataatcc gattgtttga agatgaatcg ggtttcccga aaggaacggg cggaacggta
     4261 tcaggcgtat ttgttccctt atgattgaga tgagtaaaga ttaccgaaat gatttgtacg
     4321 atgtatatgt ttcctacccg ccccaagtgg atcgcgggct tatccgggag tgcctcaggg
     4381 agaatctcgg cgaggaaaag gcggaaggat tgatcgaatc gctcgattcc aaacctcaag
     4441 tgctggttga ggaaaaatgc acttgggcga aacgggaaga gttgcacgat tatttcagct
     4501 atttgggttt ggatattatt acccggagat atagtggatt aacaaaaacc agtacggcgt
     4561 tgtctcgcct tagctcaaag agaacgattc tctaaggtgc tgaagcacca agtgaatcgg
     4621 ttccgtacta tctgtactgt ctgcggcttc gtcgtcttgt cctgattttt gttaatccac
     4681 tacatatgga gttagaaacg gtcgtgccgc cggaggaagg ggagggcgaa ggagaaggcg
     4741 cggatgggga aatgcccgaa tatcttgaac ttcatggcgg gcgggaagat gatatttccg
     4801 caccttcgca acccgaaccg ccgacccgca atatcaaacc gctgcttttc gggctgctga
     4861 ttgccttttt gggctatctg ctcggtaaga ttttttgatt gtccgataaa tgctgtattc
     4921 gggattttat atatgaaatg gttgaaacgc ctgacggtca ttgtcgggac tttttaccgc
     4981 tatcggctgg caggtctgtg tgcttcgctg atgggtagcg gttggatatg cgctctgctg
     5041 aaaatgatgc cgcagtcgtc caaattgaaa aacgaaccgc ctgccgtccg tctgcgcctt
     5101 gccttggaaa gcctggggcc gattttcatc aagttcgggc aggttttgtc aacacgcccc
     5161 gatttgattc cgcacgatta cgccgtcgaa ctggcaaagc tgcaagacaa agtgccgcct
     5221 tttgacgcgc ggctttcgcg tgaacaaatc gaaaaatcgt tgggtcagtc catcgaaaag
     5281 ctgtatgcgg aatttgaaac cgagcccatc gccagcgcgt ccatcgccca agtacacaaa
     5341 gcccgcctgc attcgggcga acgagtggcg gttaaagttt tgcgccccaa ccttttgccc
     5401 cttatcgaac aggatttgtc gctgatgcgt ttcggcgcgg cctgggtcga acgcttgttt
     5461 tcagacggca ggcgtctgaa gccgctcgaa gtggtggcgg agttcgacaa atacctgcac
     5521 gacgagttgg atttgatgcg cgaagccgcc aatgccggac agctcgggcg caatttccaa
     5581 aacagcaata tgctgattgt gcccaaggtg ttttacgact actgcaccag cgacgtgctg
     5641 acaatcgaat ggatggacgg cacgccggtt gccgacatcg ccaaactcaa agcagacggc
     5701 atcgatttgc acaaactcgc cgattacggc gtggaaatct ttttcacgca ggtcttccgc
     5761 gacggctttt tccacgcgga tatgcacccc ggcaatattt tggttgccgc cgacaaccgc
     5821 tacatcgccc tcgatttcgg catcgtcggc acgctgaccg attacgacaa acgttatctc
     5881 gccatcaatt tcctcgcctt tttcaaccgc gattaccggc gcgtcgccac cgcccacatc
     5941 gaatcgggct gggtgcctgc cgacacgcgt gcggaagaat tggaagccgc cgtccgcgcc
     6001 gtgtgcgaac cggtgttcaa caaaccgatt tcgcagattt ccttcggctt ggtgctgatg
     6061 cgcctgtttg aagtcagccg ccgcttcaat gtcgaaatcc aaccgcagct tgtgctgctg
     6121 caaaaaacgc tgctcaacat cgaaggcttg ggccgccagc tcgatcccga tttggacttg
     6181 tggaaaaccg ccaaaccgtt tttggtgaaa tggatgaacg ggcaggtcgg ccctaaagcc
     6241 ctttggcgca acctcaaaaa cgaagccccc gactgggcgc aaatcatccc ttcattgccg
     6301 cgcaaaatca gtgcgttgat tgatgaaaac cgccagcagg aaatgcgtga tgcctatatc
     6361 catttggtca aagtgcagca gcggcaaagc ctgtggctgg ctgtgattgc ggttgttttg
     6421 ctgctgattt tgcttttgaa ataggctttg tccgaatcat cgcccgactc cgcccgttta
     6481 taaggaaatc ggttatagtg gattaacaaa aaccagtacg g
//
LOCUS       DAWFJR010000104         6291 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWFJR010000104 DAWFJR010000000
VERSION     DAWFJR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.104"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..998
                     /gene="hupA"
                     /locus_tag="WGR04_001833"
     CDS             <1..998
                     /gene="hupA"
                     /locus_tag="WGR04_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupA"
                     /protein_id="HEZ0795337.1"
                     /translation="HVPVSIPTATPLTGEVKLSSDNSKIENINTANTETHPPRRTRRS
                     LYASPQNTSSGISIQQREVEKIYFGVKSPEKSFIFQTPGGAQYALSSYADPIVPSYSS
                     PDFKIPDRHAGQRLADGSRIFICCSDSGATSYAEITKQDYMKFGAWIGPNGEIDLFAG
                     GFPIGKTPPPAFSWGSPTTETALSKGKITYQVWGIRVRNGQFVTSSYTPPKGSSWSGY
                     QNTPVLSFITANFNSNKLAGEIIGNSDYGPNVKIENATIDGLSFSGDATSNGKTGNLE
                     GKFFGKFNSTRSSDTGIGGKITFKDDRSLDTVFGGVIYEKKLDDKTSQDTNHLKK"
     gene            1029..3461
                     /gene="hupB"
                     /locus_tag="WGR04_001834"
     CDS             1029..3461
                     /gene="hupB"
                     /locus_tag="WGR04_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HEZ0795338.1"
                     /translation="MPIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDIEIKNDGYVYNPTDTGGPSKYLTYVATGVARSQPDPQEWVNKSTLFKL
                     GYNFNDQNRIGWIFEDSRTDRFTNELSNLWTGTTTSAATGDYRHRQDVSYRRRSGVEY
                     KNELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDLRGINGEVYHSFRHIRQNTAQWT
                     ADFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYAPKILASNQAKITMLIENR
                     SKYKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAH
                     AGFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPELKAEKAK
                     NWELGLAGSGKAGNFKLSGFKTKYRDFIELTYMGVSSDDPNKPTYAPLSDGTKLISSP
                     VWQNQNRTAAWVKGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINAL
                     SPWTAVYSLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLF
                     DLSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSP
                     GRSYNFTIEAKF"
     gene            complement(4088..5722)
                     /gene="groL"
                     /locus_tag="WGR04_001835"
     CDS             complement(4088..5722)
                     /gene="groL"
                     /locus_tag="WGR04_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ0795339.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGTVISEEVGLSLEKATLEDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAMPDMGGMGGMGGMM"
     gene            complement(5815..6105)
                     /locus_tag="WGR04_001836"
     CDS             complement(5815..6105)
                     /locus_tag="WGR04_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ0795340.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
BASE COUNT         1520 a         1872 c         1522 g         1377 t
ORIGIN      
        1 cgcacgtccc cgtgtccatc cccaccgcca cgccgctgac cggtgaggta aagttatcaa
       61 gcgataacag caaaatcgaa aacatcaaca ccgccaacac tgaaacacat ccgccgcgcc
      121 gcaccagacg ctccctctat gcctccccgc aaaacacatc ctccggtatt tccattcagc
      181 agcgggaagt agaaaaaatc tatttcggtg ttaaatcacc agaaaaatca ttcatcttcc
      241 aaacacccgg cggcgcgcaa tacgcccttt catcctacgc cgaccccatc gtccccagct
      301 actcctcccc cgacttcaaa attcccgacc gccacgcagg gcagcggctt gccgacggca
      361 gccgcatctt tatctgctgc agcgactccg gggctacttc gtatgcggaa attaccaaac
      421 aagattatat gaaattcggg gcgtggatag gccccaacgg cgaaatcgac ctcttcgccg
      481 gcggcttccc catcggcaaa acgccgcctc ctgcgttctc ctggggcagc cccacgaccg
      541 aaaccgcatt gtcaaaaggc aaaatcactt atcaggtttg gggcatccgc gtcagaaacg
      601 ggcaatttgt tacttcctct tatacgccgc ccaaaggcag ctcttggagt ggttatcaaa
      661 acacccccgt cctttccttt atcaccgcca atttcaacag caacaaactg gcaggcgaaa
      721 tcatcggcaa cagcgattac gggccaaatg tgaaaattga aaacgcaacg attgacggac
      781 tatccttttc cggcgatgcc acctccaacg gcaaaaccgg caacttggaa ggcaagtttt
      841 ttggcaagtt caacagcacc cgcagcagcg acaccggcat aggcggcaaa atcactttta
      901 aagacgaccg ctccctcgat accgtattcg gcggcgtgat ttatgagaaa aaacttgatg
      961 acaaaaccag tcaagatacc aaccatctca aaaaataata acccatccgc ttttaaaaag
     1021 gttttcttat gcccatcccc tttaaacccg tattggctgc cgccgccatc gcccaagcgt
     1081 ttcccgcctt tgcggcagac cccgcgccgc agtccgccca aacgctgaac gaaatcaccg
     1141 ttaccggcac gcacaaaacc caaaaactcg gcgaagaaaa aatccgccgc aaaactttag
     1201 acaagctctt ggtcaacgac gaacacgacc tggtgcgcta cgaccccggc atttccgtcg
     1261 tcgaaggcgg cagggcgggt tctaacggct ttaccatacg cggcgtggac aaagaccgcg
     1321 tcgccatcaa cgttgacggg ctggcgcagg cggaaagccg ctcttccgaa gccttccaag
     1381 aattgttcgg cgcgtacggc aacttcaacg ccaaccgcaa cacttccgag ccggaaaact
     1441 tttccgaagt aaccatcacc aaaggcgcgg actcgctcaa atccggcagc ggcgcattgg
     1501 gcggcgcagt caattaccaa accaaatccg caagcgatta tgtttccgaa gacaagccct
     1561 accatttggg gataaagggc ggcagcgtcg gcaaaaacag ccaaaaattc agcagcatca
     1621 ccgccgccgg caggctcttt ggtttggatg ccttattggt ttatacccgc cgcttcggca
     1681 aagaaaccaa aaaccgctcg accgagggcg atatcgaaat taaaaacgac ggatatgtct
     1741 ataacccgac cgatacaggc ggacccagca agtacctgac ctatgtagcc acaggggttg
     1801 cgcgctccca acccgacccg caagaatggg taaacaaaag caccctgttc aagctgggct
     1861 acaacttcaa cgatcaaaac cgtatcggct ggatttttga agactcgcgc accgaccgtt
     1921 ttaccaacga gctgtctaat ttgtggacgg gtacgaccac gtctgccgca acgggcgact
     1981 accgccaccg ccaagacgtg agctaccgcc gccgctccgg tgtcgaatac aaaaacgaat
     2041 tggaacacgg cccgtgggac agcctcaagc tgcgctacga caagcagcgc atcgatatga
     2101 acacttggac ttgggacatc ccgaaaaatt acgatttgcg cggcatcaac ggcgaggttt
     2161 accattcgtt ccggcatatc cgccaaaaca ccgcgcaatg gactgccgat tttgaaaaac
     2221 aactcgactt ttccaaagcc gtttgggcgg cgcaatacgg cttgggcggc ggcagagggg
     2281 acaatgccaa ctcggattac agctatttcg caaaactgta cgcccccaaa atcctcgctt
     2341 ccaaccaagc caaaatcaca atgctgatcg aaaaccggtc gaaatataaa tttgcctatt
     2401 ggaacaatgc gtttcacttg ggcggcaacg accgcttccg cctgaacgcg ggcatacgct
     2461 acgacaaaaa cagcagcagc gcgaaagacg atccgaaata caccaccgcc atccggggac
     2521 agattcccca tttgggttcg gaacgcgcgc acgccggctt cagctacggc acggggttcg
     2581 actggcggtt taccaagcat ctgcacttgt tggcaaaata cagcaccggc ttccgcgctc
     2641 cgacttcgga cgaaacctgg ctcttattcc cgcaccccga tttctacctg aaagccaatc
     2701 ccgaactgaa agccgaaaaa gccaaaaact gggaattggg cctggcgggc agcggcaagg
     2761 cgggcaactt caagctctcg ggctttaaaa ccaaataccg cgactttatc gaattgacgt
     2821 atatgggcgt ttcgtcagac gatccgaaca aacccaccta cgccccgctt tcagacggca
     2881 cgaaattaat cagctcgccc gtctggcaaa accaaaaccg caccgccgcc tgggtgaaag
     2941 gcatagagtt taacggcact tggaacctcg acagcatcgg gctgcccaaa ggtttgcata
     3001 ccggcctcaa cgtcagctac atcaaaggca aggcaacgca aaacaacggt aaagaaacgc
     3061 ccatcaacgc gctttcgccg tggacggcgg tttacagctt gggctatgac gcgccttcca
     3121 aacgctgggg catcaacgcc tacgccacgc gcaccgccgc caaaaagccg tccgacaccg
     3181 tccacagcaa cgacgatttg aacaacccgt ggccttatgc caaacacagc aaggcctata
     3241 cgctgttcga cctttccgcc tacctcaaca tcggcaaaca ggttacgctc cgcgccgccg
     3301 cgtacaacat taccaacaag cagtactaca cttgggaatc tttacgcagc atccgcgaat
     3361 tcggcacagt caaccgcgtt gacaacaaaa cccacgccgg catccaacgc tttacctcgc
     3421 cgggcaggag ctacaatttc accatcgaag cgaagttcta agctcctgat gtgccaatgc
     3481 cgtctgaaac ccgatgattt gggtttcaga cggcattttt tacggtcgca ccgccgcccc
     3541 ctttcaggct tgtaccaagc ccaatcccgc aactctccgt cattcccgca actctccgtc
     3601 attcccgcaa ctctccgtca ttcccgcaac tctccgtcat tcccacgaaa gtgggaatct
     3661 agaaacgcaa ggctgcaaga atttatcgga aatgactgaa actcaacgaa cctggattcc
     3721 cgcctgcgcg ggaatgacga agctatccgc acagaaacct gcaccacgtc attcccacga
     3781 aagtgggaat ccagaacgta aaatctgaag aaaccgtttt atccgatacg tttccgcacc
     3841 gacagaccta gattcccgct ttcgcgggaa tgacggcgga aaggttgctg tttttccgat
     3901 aaattcctgc cgctcttcgt ttttgggatg gcgggaaata aaacaaaagc gcgcgtatca
     3961 aaaaacaaca atgcaaagaa cggcgttggc aattttaagg tttgcgcctt agccgcactt
     4021 attcgcaaaa aaaaccgcac ggcgttgacc gtgcggcttg ttgtctgaag gcttcagacg
     4081 gcattgctta catcatgcca cccataccgc ccatgccgcc catatcgggc atagcaggtt
     4141 tttcttcagg gatttcggca atcatgcaat cggtcgtcag catcaggccg gcgatggacg
     4201 cggcgtgttg cagcgcggaa cgggttactt tggcggggtc gagtacgccc atttcgatca
     4261 tgtcgccgta ttcgccggag cctgcgttgt aaccgtagtt gcctttgcct tccaacactt
     4321 tgttcaccac cacgctaggt tcgccgcctg cgttggcaac gatttggcgc agcggagact
     4381 caacggcgcg caagacgatt tgtacgcctg cgtcttggtc ggcattgccg gtgtgcaggt
     4441 tttccaaagc agcacgggca cgcaacaggg ctacgccgcc gcctgcaacc acgccttctt
     4501 caacggctgc gcgggtagcg tgcagcgcgt cttccacgcg gtcttttttc tctttcattt
     4561 caacttcggt cgcggcaccg actttgatca ctgccacgcc gcctgccaat ttggcaacgc
     4621 gctcttgcag tttttctttg tcgtaatcgc tggttgcggt ttcgatttgt tggcggattt
     4681 cggcaacacg cgcttcgatt tgggctgcgt cgccgaagcc gtcgatgatg gtggtgtttt
     4741 ctttaccgat ttcgatgcgt ttggcttgtc ccaagtcttc caaagtcgct ttttccagag
     4801 acaggccgac ttcttcggaa atcaccgtgc cgccggtcag gatggcgatg tcttgcaaca
     4861 tggctttgcg gcggtcgccg aagcccgggg ccttaacggc aacggttttc aggatgccgc
     4921 ggatgttgtt cacgaccaaa gtcgccaagg cttcgccttc tacgtcttca gcgataatca
     4981 agagcggacg gctggctttg gccacttgtt ccaaaacagg caacaggtcg cggatgttgc
     5041 tgattttttt gtcgaacaac aatacaaacg gattgtccaa agcagcgatt tgtttttccg
     5101 cgtcgttgat gaagtaagga gacaggtagc cgcggtcgaa ctgcatacct tcaaccacgt
     5161 ccagctcgtt ttccaaagat ttgccgtctt caacggtaat cacgccttct ttgccgactt
     5221 tttccatcgc ctcggcgata atcgcgccga cttgttcgtc ggagttggcg gaaatcgagc
     5281 cgacttgggc gatttctttg gaagtgtcgc aaggtttggc gatgtttttt agctcttcaa
     5341 ccaaagcggc aacggctttg tcgataccgc gtttcaggtc ggtcgggttc ataccggcgg
     5401 taacgtattt cataccttcg gcaacgatgg attgcgccaa tacggtggcg gtagtcgtac
     5461 cgtcgcctgc cacgtcgttg gttttggacg cgacttcttt caccatttgc gcgcccatat
     5521 tttcaaactt gtctttcagt tcgatttctt tggcgacggt tacgccgtct ttggtgatgt
     5581 gcgggccgcc gaaagcgcgg tcaacgacta cgttgcggcc tttggggccc aaggttacgc
     5641 gcacggcgtt tgccaaaatg ttcacgccgt ttaccatttt ttggcggact tcattgccga
     5701 actgtacgtc ttttgctgcc atttcaattc tccaaaaatc attaaaactg tctgataaaa
     5761 ccgtttatgc cgtctgaagg cggtttgccg tttcagacgg catcgtgtcc gtatttattt
     5821 ttcaacgatg ccgaaaatat cttcttcacg cattaccaac agctcttcgc cgtcggcttt
     5881 gacggtttgg ccgctgtatt tgccgaaaat gattttgtcg ccgactttga catccagcgg
     5941 acggcgcgcg ccgtctttgc cgattttgcc cgcgcccacg gcgatgactt cgcccatatc
     6001 gggtttttcg gcggccgcac ccggcaaaac gatgcccgat gcggttttct cttcagcttc
     6061 caagcgtttg acgacaacgc ggtcgtgtaa aggacggatg gtcatattta tgctccgata
     6121 aaatgatttg aaaacaatca tctgcccgaa cggttcggac agattgaagt ggaaaccggg
     6181 ctgccgcctg ccggcccgcc cgtataagtc ggcaaaatta gggtgtgtgc gggcaaattc
     6241 aagtgaggcg gaaaaaattt atttccgccg ttttttatag tggactaaat t
//
LOCUS       DAWFJR010000105         6260 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWFJR010000105 DAWFJR010000000
VERSION     DAWFJR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.105"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(392..826)
                     /locus_tag="WGR04_001837"
     CDS             complement(392..826)
                     /locus_tag="WGR04_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ0795341.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(829..1491)
                     /gene="exbB"
                     /locus_tag="WGR04_001838"
     CDS             complement(829..1491)
                     /gene="exbB"
                     /locus_tag="WGR04_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ0795342.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1557..2399)
                     /locus_tag="WGR04_001839"
     CDS             complement(1557..2399)
                     /locus_tag="WGR04_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ0795343.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPEPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPSNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2654..3292
                     /locus_tag="WGR04_001840"
     CDS             2654..3292
                     /locus_tag="WGR04_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ0795344.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3744..4544)
                     /locus_tag="WGR04_001841"
     CDS             complement(3744..4544)
                     /locus_tag="WGR04_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ0795345.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(4680..5183)
                     /locus_tag="WGR04_001842"
     CDS             complement(4680..5183)
                     /locus_tag="WGR04_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795346.1"
                     /translation="MDAFDFFMQGNFKKIATEQGLDRKYFIFQYIVCNLSAISSFCLM
                     KIIHTYTILYILIAVIGIINGFLLRSKVLSIVKNKQKFLSDMFPLEMPFFFVEYKIPI
                     LARFLNRGMKWLYLPSFFCLVAICFSIYILNEEGGWMPYFACYWFGLCYPVSTYYVFL
                     SRDYKHI"
     gene            complement(5520..6161)
                     /locus_tag="WGR04_001843"
     CDS             complement(5520..6161)
                     /locus_tag="WGR04_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ0795347.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
BASE COUNT         1669 a         1567 c         1302 g         1722 t
ORIGIN      
        1 gggtatagcg gattaacaaa aaccagtacg gcgttgcctc gccttaactc aaagagaacg
       61 attctctaag gtgctgaagc accaagtgaa tcggttccgt actatttgta ctgtctacgg
      121 cttcgttgcc ttgtcctgat ttttgttaat ccactataaa aattagaaat gcacattttc
      181 attattctcg cgcaggcagg actccagact tacccatttc agtaatgttt gaaaataaaa
      241 gaaaaatcag atgtttgtat tcccgcctgc gcagaaatgg agacggcgct ctgtcgtctc
      301 atttttgttt taatcaacta tatatagctg attaaacata agaaatgccg tctgaaagac
      361 tttcagacgg cattcgttca agcgtcgaac tttattgcgc cttggtttcg gttacaaaac
      421 cgattttggt gattcctgcc tgacgggcgg cttctaaagc tttgtttaca taatcgtatt
      481 ccaccgcctt gtctgccgca atcgccacaa tcacgttttc attctgctcc ttggcggctt
      541 tcagacggct ttccacttcc ccgatttcca ctttgcttgc agaatccccg ccgacataat
      601 agccgccgtt cgcatcaatc gtcaggcgca gggggtcttt aggctgtttg tcctgcttgt
      661 ttgtctgctc ggacgcggtc ggcagttcca aagggatgga atgcgtcagc accggcatag
      721 taatcataaa cacaatcagc aacaccagca tcacgtccac caacggcgta acgttgatgt
      781 cggacatcgg agaatcgtcg ccggaattca tcgaaccaaa tgccataatc agctatcctt
      841 ttgattaagc aggcggacgt gcaaatcgtg cgccatcgca tccaaatcct gggtcagtat
      901 ttttgtgccg cgattgagga agttgtatgc caacaccgcc ggaatcgcca cgaacaaacc
      961 cgccgccgtc gccaccagtg cctcgccaat cgggccggca accgccgcaa tactcatctg
     1021 cccgctttgc ccgatattga tcagggcgtg gtaaatcccc caaaccgtgc cgaacagccc
     1081 gataaacggc gcggtcgcgc cgatggaggc aagcgcggtc atcccgtaat caaaccggcg
     1141 cataatctgc gccatactgt tgcggatttg aatgaccaaa tactcgttca acggcaaagc
     1201 ctgcgccagt tcggacgctt cgtttcggcg gtagttgcgg taagactgca atgcctcttg
     1261 cgccagtttg gacaaaggcg catcgacggc gcgcactttt tcgaccgcgt cgttcagcga
     1321 caaagtatcg cgcatatgcc gtttgacggc ggcattccct ttgcgcgccc gatacagctt
     1381 gatgcagcgc aagacaacca aacaccacgt tacgatactc atcaacagca tcaacacaaa
     1441 cacaccgatc aggacgggat cgcccgattc aaatactaat ttcaaattca taatgattcc
     1501 aacactgaaa aaaccaatca aacatccaag ctgccgcaaa ccgctgcggc aaccgcctaa
     1561 ttcaattcaa acttgacggg gactttaaac tccgtccagg cattggcttg aaaatgcccg
     1621 ttttgcgccg ccttgcgtgc cgcattgtcc aaccgagaaa aaccactgct tttcacgatt
     1681 ttaacggact cgacatgacc gcccggagaa accaaaacgc tcaaaacaac cgtaccctgc
     1741 tcgtcattct ccatagaaag cgtgggataa gccgggcgcg gaatgctgcc gttggcgcgt
     1801 aaaggattgc ctttgctgct gccggctcct tccccgtgtt cgcctttgac accgccgcta
     1861 cctttaccgc tgccttctcc gcgccccgtt ccgtctcctt tggtaccggt tcccttatct
     1921 tccccattac cctgctcgct gtctgctttg gcagaagcat tgctgggatg ttcggcaggt
     1981 ttttcagacg gcttctcgac cggtttttcc gccggtttcg ggacaggctt cgcttccggc
     2041 ttaggctctg gtttcggttt ttcttcgggt ttcggctttt cttcaggttt cggctcttcc
     2101 ttaggctgct gaatatccgc atccgccttt ttcgtaacca ccggcttcaa aaccggcttg
     2161 ggcggctcga caggtttggg cggctcaggc tcaggttgcg gctcaggcgc agcaggcgca
     2221 cctgcgcctt cgggtgcacc gtcccctccg ccaaaatcac ccaaatcgac aaattcgata
     2281 acatttcctg actctatcac gggcagcttg tgcgcctgcc agagcaatgc caccattgcc
     2341 aaatgcagca gtgcgacgga aaacacgact gcgggggtta aaattcgttc tttatccata
     2401 attcgggcat aataatagca acaattccta tttgcaacct atttttacaa tttttggtca
     2461 tatgaatgtc tgttccgttc acaggcaaac cgtgtttaaa cgctgtatta cagcaaatca
     2521 tcagataacg ggccggcaga aaaaatgatt ccgtctgatt tcttattcca ataaaatcag
     2581 gttagatgat atattgccgc ttctgtctgt cagccgtttc gggctgcaca ccacatctgt
     2641 tcaaaggaaa accatgtttc aaaattttga tttgggcgtg tttctgcttg ccgtcctgcc
     2701 cgtgctgctc gccattaccg tcagggaggt ggcgcgcggc tatacggcgc gctactgggg
     2761 agacaacact gccgaacaat acggcaggct gacactgaac cccctacccc atatcgattt
     2821 ggtcggcaca atcatcgtac cgctgcttac tttgatgttc acgcccttcc tgttcggctg
     2881 ggcgcgtccg attcctatcg attcgcgcaa cttccgcaac ccgcgccttg cctggcgttg
     2941 cgttgccgcg tccggcccgc tgtcgaatct ggcgatggct gttctgtggg gcgtggtttt
     3001 ggtgctgact ccgtatgtcg gcggggcgta tcagatgccg ttggctcaaa tggcaaacta
     3061 cggtattctg atcaatgcga ttctgttcgc gctcaacatc atccccatcc tgccttggga
     3121 cggcggcatt ttcatcgaca ccttcctgtc ggcgaaatat tcgcaagcgt tccgcaaaat
     3181 cgaaccttat gggacgtgga ttatcctact gctgatgctg accggggttt tgggtgcgtt
     3241 tattgcaccg attgtgcggc tggtgattgc gtttgtgcag atgttcgtct gactggcttt
     3301 cagacggcat aaacgctcca gaaaacgcgg caggacatat tgccctgccg cgttttcctg
     3361 tagtgtaatc ttattttttt catcattatt agaaccaggt tgcatgataa tacctttcat
     3421 taactgaaac actgattaag aaactccagt ctgtctaatg atgaggtttt cacatcgcca
     3481 aaacttgcca atcaaatgct ggatttattg ccgtctgaga tttggtcaaa tccaaaggcg
     3541 acattcttag accctgtgtg taaatcaggg gtatttttgc gtgaaatcgt caaacgcttg
     3601 gatgaaggct tgaccaatca aataccagat aaacaaactc gcattaacca cattttaaaa
     3661 aatcaagttt ttggaagtac tgccacgtat gtaggtagct ttgaccgata tttgcataaa
     3721 aactcctttg ctggtgaaag gaattatttt gccaatttta aaatatttct ggcaccaaat
     3781 agtacaatga caaagacaat catgccaatg attaaatcag gatagctaga atgagtcaat
     3841 aacgtcaatg ctcccgccgc tatcacaccg atattgatga taatgtcatt ggatgtaaaa
     3901 atcatgctgg ctttgatatg gatttcttta ttttgatttt tgctcagtag atataagcac
     3961 agccagtttg caatcaatgc caaaaatgcc gtgccaatca tcagttgata attgggcagc
     4021 tgctcagcac cgataaaacg cctaatcact tctatcaccc caaataacgc caatattatc
     4081 tgcgttatcc ccgccaaaaa tgccacacgt tttttatacg ccagcgtcat accaatggct
     4141 gatagcgcca atatatagac aaagctgtcc gccagcatat ctagactatc agcaatcagc
     4201 cccatagaat tagcaaaaat accaaccgaa cactctatga taaaaaacac aaagttaatc
     4261 atgagcactt gatataataa tcttttttct aagtgctcat caggcttgtt aaacactatc
     4321 ttatcaacaa tcacttcggt ggaaatgata tgactatcaa aattaagcgg ttcaagtact
     4381 tgtaaaatcg ttgtatcttg attatcgtga tagacggtta agcaccgccc agcaatatca
     4441 aactgtaatt cataaatatc agacacatct tttaaacgca tgcgaatgag ctgttcttcg
     4501 gacgggcagt ccattttggt aatgttaaaa atggtctttt tcatctattt agttccttgt
     4561 tttgatcagg ttggctcaaa taaatctgtg tttatattgc tgcttggtaa tttttggatg
     4621 gtttgagtaa attgattagg ttaaaattta cctttggaag taccgccacg cataatagtt
     4681 tagatatgtt tataatctct ggataaaaaa acgtaataag tgcttactgg ataacaaagt
     4741 ccaaaccaat agcaggcaaa ataaggcatc cacccccctt cttcattaag gatatatatt
     4801 gagaaacaaa tcgcaactaa acagaaaaaa cttgggagat aaagccattt cattccccta
     4861 ttcaagaatc tagccaagat aggtattttg tattctacaa aaaagaaagg catttccaag
     4921 ggaaacatgt cagataaaaa cttttgttta ttttttacta tagatagaac cttgcttctc
     4981 aagagaaagc cattaataat accgatgaca gctattaata tatagagaat agtataagta
     5041 tgaataatct tcattagaca aaaagaagaa atggcagata aattacatac gatatattgg
     5101 aatataaaat atttacggtc taaaccttgt tcagttgcaa tttttttaaa attgccttgc
     5161 ataaaaaaat caaaggcgtc cattaaacta tctttcacat tagaaattta aaagctaaat
     5221 aatacgacaa acaatgtgaa gtactattca tggtttattt aaaaaataat actattctga
     5281 acattattta gatacagaaa ttaacaaatt agaactaaac aagcttttaa atactttaat
     5341 tttattggaa agctataaaa ggaactataa ctttacacac tagtcacttc tttttaagag
     5401 gcaaaaggga ttgggaaggt cgtcttggag ataagcactg gtatttcggc caatggtaaa
     5461 tagagtttac ctcaaatagg gtagaacctc cttcatctgt cagttaataa cagccacttt
     5521 tacaatgccc tgtcgaaata aagtggcacg cccgattttt cactcatacc gattaaataa
     5581 tccatcgtct tccgagggaa cgcgatgccg cgcaccacgg tcagattcct caaaacgggg
     5641 aaaaccaaaa tatcctccat accgattccg ccgttgatgc cgtctgaagc accgtccatc
     5701 aaattttcca actcttgcaa atctgcgttt atccgttcga ggtattgggc ggttttattc
     5761 aaattggcgg aaaagctgcc gatgcttttc tcttttttgt ctgtaaaata tttcaccgct
     5821 tccggcgttg caaattcagg cagcccgatt ttgatcacgc gcggctgcac cagtttgtcg
     5881 ttgtatccgc ccaccttgtc cagccacgcc cgtatctcgg ggcggacttc gtctttcaga
     5941 cggtcttcgc ggtcgaaatg ccgcacaatg tccaaactct cgcccataaa cgaaccgtct
     6001 tctttttgca ggacgggcac ttgtttcgca ccgatcatac cgatcggcgt tgcctcgtcg
     6061 tcgtttgcca gcacggcttc ttcaacgtcc gcgccaaaca gcccggcagc catccgcgca
     6121 cgcacgcaaa acgggcaatg gtcgtaaata tacagtttca tcaaaatatt cctcgtcaac
     6181 ctgtcggtac cggctacctt aacaccccgc gccacccgaa acaagtttat cttcccgcct
     6241 atgcaccgta aataaataag
//
LOCUS       DAWFJR010000106         6232 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWFJR010000106 DAWFJR010000000
VERSION     DAWFJR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6232
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.106"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            152..1705
                     /locus_tag="WGR04_001844"
     CDS             152..1705
                     /locus_tag="WGR04_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ0795348.1"
                     /translation="MNRTYANFYEMLTAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAISAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPTGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEVKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(1795..3822)
                     /locus_tag="WGR04_001845"
     CDS             complement(1795..3822)
                     /locus_tag="WGR04_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ0795349.1"
                     /translation="MSLSEFIERRTSFNLMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHRDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            4111..6117
                     /locus_tag="WGR04_001846"
     CDS             4111..6117
                     /locus_tag="WGR04_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ0795350.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTTAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
BASE COUNT         1591 a         1887 c         1451 g         1303 t
ORIGIN      
        1 tagctcatgg caatctttct tgcaggaaag gtcgtatgct accgcatact ggcctttttc
       61 tgttagggaa agttgcactt caaatgcgaa tccgtcggca tattgttttc aaatgagggc
      121 gttctccgtc cgcaaccata aaggaagttt catgaaccgg acttatgcca atttctacga
      181 aatgctgacc gccgcctgcc gcaaaaacgg caacggcacg gcagtgttcg acggcaagga
      241 aaaaaccgcc taccgcgcgc tcaagcagga agccgaagcc gtcgcggcgt atctgcaaaa
      301 tatcggcgtg aagttcggcg acacggtcgc gctggcggtt tccaattcca cagaatttat
      361 taccgcctat ttcgccatct ccgccatcgg cgcggtcgcc gtaccgatga acacattttt
      421 gaaaaacagc gaatacgcgt atatcctgaa cgactgcaag gcgcgcttcc tgttcgcctc
      481 ggccggcctg tcaaaagaat tggcgggctt gaaggcgcaa acgcccgtcg aaaaaatcat
      541 ttggacggac aaaagccgtc cgaccggcga aatggcggaa ggcgatgcct tttttgaaga
      601 cgtgcgccgc ttccccgaaa aacccgactt gggccgccaa ccccggataa atgatttggc
      661 acacatcatc tacacctccg gcacgacggg gcatcccaaa ggcgcgctaa tcagttacgc
      721 caacctgttc gccaacctga acggcatcga acgcatcttt aaaatttcca aacgcgaccg
      781 ctttatcgtt ttcctgccga tgttccacag cttcacgctg acggctatgg tgctgctgcc
      841 gatttatatg gcgtgttcga ttattttggt caaatccgtt tttccgtttt ccaacgtttt
      901 gaaacaggcc ctgctcaaac gcgcgaccgt gtttttgggc gtacccgcga tttacaccgc
      961 gatgagcaag gcgaaaatcc cttggtattt cagatggttc aaccgcatcc gcctgtttat
     1021 cagcggcggt gcgcctttgg cggaacaaac catcctcgat tttaaagcca agttcccccg
     1081 cgccaaattg ctggaaggct acggactgag cgaagcctcg cccgttgtcg ccgtcaatac
     1141 gcccgagagg caaaaagccc gcagcgtcgg catccccctg cccggtttgg aagtcaaagc
     1201 cgtcgatgaa gaattggtcg aagtgccgcg cggcgaagtg ggcgaactga tcgtcagggg
     1261 cggttcggtg atgcggggct acctcaatat gcctgccgcc accgatgaaa ccatcgtcaa
     1321 cggctggttg aaaacgggcg atttcgttac catagacgaa gacggcttta tctttatcgt
     1381 cgaccgcaaa aaagatttga ttatttccaa aggtcaaaac gtctatccgc gcgaaatcga
     1441 agaagaaatc tacaaactcg atgccgtcga agccgccgcc gtcatcggcg tgaaagaccg
     1501 ttatgccgac gaggaaatcg tcgccttcgt ccaattgaag gaaggtatgg atttgggcga
     1561 gaacgaaatc cgccgccacc tgcgtaccgt gctggcaaat ttcaaaatcc ccaaacaaat
     1621 ccactttaaa gacgggctgc cgcgcaacgc tacgggcaag gtattgaaac gggtgttgaa
     1681 ggagcagttt gacggaaaca aatgaacgcc gtgccgtccg aagccccgtc cggcaaaaaa
     1741 atgcggtgaa tctgcttcac cgcatttttt tggcaaacgc cggagataag gcacttactc
     1801 tgccaattcc acctgctcgt gcgccatcag ttcctgaccg acatcatcca acaacatcaa
     1861 ataacgttcg tagtttttca aaatgtcggc aatcagctcg tccttgttca tatactgcac
     1921 atcgtacccg acgcgcccgt cgaaaaaata agcgtagggt ttgtaagttg tctggtgccg
     1981 gatatgcggc agcttgccgt cgttaatcaa ctggtcggat acatcctgcc cgacagattt
     2041 aatcccgtac ataaaatcgc gcatcgtctc tttccgaatg acgaactcga ttgcgggctc
     2101 gtcccgatga aacattttat cgacccggac gctcaagccg tattcttccg aaagctcccg
     2161 ttgcaactcg tgcatagcgg gcgatgcggt atgtttgagg aattttaaaa tatcctgctc
     2221 ctgcgtctgg ctcattatct gcaccagccg ttctttccac ttgccgcccg tccaaaatac
     2281 actggtaggg ttaacccggg tctcaaaata tttcttatcc gcactcaagc ctttccacag
     2341 gctgaaacac attatcagca tcagcagggc aaacggcagg gaaacaatca gggtcataga
     2401 ctgcaggttg ccgagtccgc ccgagcgcat cagcaaaacg gcaacggcag acatcagcac
     2461 gccccacata accgcctgcc accgtggcgc gctcaagcct ttgtcccgag aggtaatatt
     2521 gttcaggaca taaatcccgg aatcggcaga agttacaaaa aacagagaaa tgaccagcag
     2581 gctgacgatg ctcgtcaatt cgggcagggg gaggtaatta aagaatttaa aaagcagcgt
     2641 ttccggagag gaggtcatct tttcgagcat tccccccgca accccgtcat tcagccaaat
     2701 cgccgtattg ccgaagacgg taaaccacaa aacgccgaac aggccgggga tgagcaaaac
     2761 cccgaagaca aactcgcgga tggtgcgccc ctttgaaatg cgcgcgataa acaaacctac
     2821 aaacggcgcc caagaacacc accacgccca ataaagcacc gtccaagatt caaaccacgg
     2881 cttgtgttcc cgttcgtacg cataagtttt aaaactgagg cgcaccagat ttccgaggta
     2941 gttccctatg ttgtcgccga atgccgacaa caggtaaaca gtgggacccg ccgccaaaac
     3001 aaaaaacagc agcaaaaacg caaggctcag gttcaactcg ctcaacacct tcacgccctt
     3061 ccccacgccg gatattgccg aaacgacggc gagggacatg acggcggcga taatcaaaac
     3121 ctgcacgctg aagctgtttt cggcaatcca gcccatttcc tgcaatccgg cgcccagttg
     3181 cgaagccccg aaccccaatg tggtgatgat gccgaaaaaa gtagcaagca acgccataat
     3241 atcaatggca tcaccgaacc ttccggaaat tttttctttc aacagggggt aaaaacaaga
     3301 acgcagggca agcggcagct tgtagcggaa accgaaataa gccaaagcca atgcaatcgt
     3361 accgtacacc gaccaagcgt gaacgcccca atggaacacc gtgtgcagca atgcctgctg
     3421 ctgcctgtgt tccggcgtgc cggccgtaat gtccgaaaaa taatgcatca acggctctgc
     3481 cacgccgaaa aacatcagac ccacgcccat cccggccgca aacagcatcg ccagccacga
     3541 caggaagccg aattccggca catcttcatc ccgtccgagc ctgatgtttc ccaaactgct
     3601 gaccgagagt atcagcagga aacccagaaa aatggaaaac gttaaaacat aaaaccagct
     3661 gaactcggta aaaatgactt cttttgcccg atcgagccac atctgcacct gatccggcac
     3721 ggttaaaacc aataccacta aaacacacac aaaaaacaaa gtcgtcaaaa taaccatcag
     3781 attaaatgac gttcggcgtt ctataaattc agacagggac aaaccttctc actcctttgt
     3841 taaaaacaga caaacccggt catcgggcaa agcggtcaaa acctgccggt aaaataccgg
     3901 cttccggatg acgaaatgca caaaccggcc gaaattgtta taatcgaaaa aattaaaaat
     3961 caatacggat tattctatca gattgattta tcgatattta ttatatcatt aatatagttt
     4021 gatttcaaac cggcggaaaa tgcgccgcct tccgcaggcg cgccctcccc gctatcgggg
     4081 cgtttaacgc cggattgccg atgcggtaca atggctgata tgaagaaaat tacccctcaa
     4141 aacctgcgcc ccctgctttc ggaaagcttg ggacataccg attttgtcaa cgtcctcaac
     4201 gcactgatta aatttttgcg ccgtggcggc aaaaaatgtg cgggggaacg tttcgacctg
     4261 attatcgaca cattcaaaca agacagggaa ttactgtccc gcttcagccg gtgtttttac
     4321 atctggctcg cgcaaataca catttatccg gcactcatca aactcggcat cttctcgcgc
     4381 cacagctttg cccgggaaat gggcatacgc atctacgaac gcttcagccc gtcatataaa
     4441 gattttgcca acttgggcga agtcttcctt tatcttttcc attccgaaaa cgacgacaaa
     4501 tggctgcaaa cgctcaatat ccgccaatgg ctggttttat acgaactcat ccggagccac
     4561 gccgagccgt ccaaattgca gacggcgggc atccgccttg ccgatgcgcg tttgcgcgcc
     4621 atcgaaatgc tgtctgtctg gacggcatcc gaagccatcg aacccgacct catccgcatc
     4681 gccccgcgcc tgctggaagc cgattcttcc ttcgtcgccc tccaacgcga aaccgccaaa
     4741 ctggtcgaac actaccgcaa cggcaccacg ccttacgaca ccgcccacct cgaagtgatg
     4801 ttcgaccaat gtttcagcca gattgactat ttgcgccgca aagggacggg cgccggctcc
     4861 ggttcgtcgg tcaaagtcgc ccacctgctc gaacggctcc ggcagaccgt agaccgtcta
     4921 aagctgctca ccgacatcca aaccggtgcc ggcaacagca accgcctgac catcgccctg
     4981 atgaactccc tcatctacgc ggcggtcgaa caatacagca cccgccacct gcgccgcagc
     5041 agcatccgta tgctcgcccg cagcattacc gaaaacaaaa gccaccacgg cgaacactac
     5101 atcacccgca accgcaaaga atatttcaaa atgttctact cggcggcagg cggcggcatc
     5161 atcatcgccc tgatggcgct gctcaaaatc cgcatcggca cactcggcct cagccccttc
     5221 ctcacttcct tgtcggcggg gttcaactac ggcatcggct ttatgatcat ccatatgctg
     5281 cactgcaccg tcgccaccaa gcagcccgcg atgactgccg ccagctttgc cgaacaggtc
     5341 gatctcaacg aaggcggcaa agcggtggac aacaaactcg ccaagctcct catcgacgta
     5401 tgccgctccc aaagcgtcgc cgtcttcggc aacgtttcca tcgccatcct tttggcgtgc
     5461 gccatatcgt tcggctatgc ccatctgtac cggctgccca tactcgatgc ccacaccact
     5521 gcctaccagt tcaaatccat agacatcatc gcttacccga cgctgtggta tgccgccatt
     5581 gcaggtctgt ggctgttctg ctccggcatc atcgcaggtt ttttcgacaa ccgcgccgac
     5641 tacctcaacc tgcgccaacg cctgcccttc aaccccttgc tgcgtaaaat catgcgcccc
     5701 gggcctcgcc gcgtcctcgc cgcctacatc cacaaacact acggctcgct ggcaggcaac
     5761 ttcatcttcg ggatgctctt aggtatgacc ggctatttcg gacacctcct cgggctgccg
     5821 ctggacatcc gccacgtcgc cttttcctcc gccaacctcg gctatgccgc cgtcagcggc
     5881 aacgtcggtt tgggcacgtt cgtactcggc attttcagcg tcctcgccat cggcttggtc
     5941 aacctctgcg tcagtttcag cctcgccctc ttcgtcgccc tgcgctcgcg cggcacgaaa
     6001 atcggcagta tccgcaatct gattaaaagt ttttggaatc agattaaaag caatccctgc
     6061 atacttttcc tcccgcccgc caaagaacag ggacatcctc cttcggacaa gccttgaccg
     6121 gcaatgccgt ctgaagcggg attcgccccg aataccgccc tgatgcggga aatccccata
     6181 aaaggatgca aaaatgccgt ccgaaccgaa acgtggttca gacggcattt ta
//
LOCUS       DAWFJR010000107         6105 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWFJR010000107 DAWFJR010000000
VERSION     DAWFJR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.107"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            25..408
                     /locus_tag="WGR04_001847"
     CDS             25..408
                     /locus_tag="WGR04_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ0795351.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            522..995
                     /locus_tag="WGR04_001848"
     CDS             522..995
                     /locus_tag="WGR04_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ0795352.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKTAPMPAMNHGHHHGEAHQH"
     gene            1121..2224
                     /gene="mnmA"
                     /locus_tag="WGR04_001849"
     CDS             1121..2224
                     /gene="mnmA"
                     /locus_tag="WGR04_001849"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ0795353.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIVGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFKLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDDETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            2295..3965
                     /locus_tag="WGR04_001850"
     CDS             2295..3965
                     /locus_tag="WGR04_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ0795354.1"
                     /translation="MKKIWLDSYEKGVSAEIDITQYNSISDVFRQSVEKFARLPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGQLKKQRVNVCIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            4077..5711
                     /locus_tag="WGR04_001851"
     CDS             4077..5711
                     /locus_tag="WGR04_001851"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ0795355.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGVHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSGHIRERHRHRYEVNNNYVPQLEKAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
     gene            5939..6105
                     /locus_tag="WGR04_001852"
                     /pseudo
     CDS             5939..6105
                     /locus_tag="WGR04_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         1604 a         1675 c         1527 g         1299 t
ORIGIN      
        1 tgagaacatt ttgaaaggat accgatggaa ccttcctcct acgcggcaga aaaaaaaggc
       61 aaaggcggca tcaggcgcgt cattaacgca ttcggctatt cgatagacgg catcgccgcc
      121 gcctaccgtt acgaagcggc attccgtcag gttttgtggc tgaacgtact gctggtgtgc
      181 gcggcatttt tttgggtttc cgaaaccgcc gtccgcctgc cgttgattat cgcgtctttt
      241 gtgtcggtca ttgtcgaact gttcaacacc gccgtcgaag ctgccgtcga tcatacttcg
      301 accgaaaaac acgagctggc caaacgcgcc aaagacgcag gttctgccgc acaattgttc
      361 gcgatgctga tgttggcggc ggtttggctg tctgccctgt tcgggtaaaa cgcttgcagc
      421 aggatttgta atcctttagg attagtatgt ggtatttatt attcacttaa tccactataa
      481 gattaagcac attccccgtc tgatattcaa ggagcagaaa gatgaaaaaa ttattggcag
      541 ccgtgatgat ggcaggtttg gcaggcgcgg tttccgcggc cggagtccac gtcgaggacg
      601 gctgggcgcg caccaccgtc gaaggtatga aaataggcgg cgcgttcatg aaaatccaca
      661 acgacgaagc caaacaagac tttttgctcg gcggaagcag ccccgttgcc gaccgcgtcg
      721 aagtgcatac ccacatcaac gacaacggcg tgatgcggat gcgcgaagtc aaaggcggcg
      781 tgcctttgga ggcgaaatcc gttaccgaac tcaagcccgg cagctatcac gtgatgttta
      841 tgggtttgaa aaaacaatta aaagagggcg acaagattcc cgttaccctg aaatttaaaa
      901 acgccaaagc gcaaaccgtc caactggaag tcaaaaccgc gccgatgccg gcaatgaacc
      961 acggtcatca ccacggcgaa gcgcatcagc actaatctgc tgaaaatatt tgaaatgccg
     1021 tctgaaaaag cccgggcttt cagacggcat ttttatgacc gcctttaaaa tgtgttaaaa
     1081 tctgccttta aaaaccgccg tttccaacca tattctacgc atgaatacga cagcaaaccc
     1141 ttccaatatc atcgtcgggc tctccggcgg tgtcgattct tccgtaaccg ccgccctgct
     1201 caagcagcag ggttatcaag tgcgcggtgt gttcatgcag aactgggaag acgacgacaa
     1261 cgacgaatat tgcagcatca aacaggattc gtttgatgcc atcgccgttg ccgatattgt
     1321 cggcatcgac atcgacatcg ttaatttcgc cgcgcaatat aaagacaaag tctttgctta
     1381 ttttcttcag gaatacagtg cggggcgcac gccgaatccc gatgtgttgt gcaacgccga
     1441 aatcaaattc aaatgctttt tggattacgc cgtagggcag ggcgcggata ccatcgccac
     1501 cggacactat gcgcgcaaag aagtccgcaa cggcgtgcat tacctgctca aaggtttgga
     1561 tcgaaacaaa gaccaaagct atttcctcta ccgcctcaag cctttccaac tcgaacgcgc
     1621 gatttttccg ttgggcggtt tggaaaaacc cgaagtgcgc cgccttgccg ccgagttcaa
     1681 actgccgacc gccgccaaaa aagacagcac gggcatctgc ttcatcggtg aacgcccgtt
     1741 tcgcgagttt ctgcaaaaat acctgccgac cgacaacggt aaaatggtta cgcccgaagg
     1801 gaaaaccgtc ggcgaacacg tcgggctgat gttctacacg ctcggacagc gcaaaggttt
     1861 gggcatcggc ggcgcgggcg aaccgtggtt tgttgcggct aaggatttga cgaaaaacga
     1921 actgattgtc gtgcaaggac acgaccatcc gctgctctat acccgcagcc ttgtgatgaa
     1981 cgatttgagt ttcacgctgc ccgaacgccc gaaagcaggg cgttatacct gtaaaacgcg
     2041 ttaccgtatg gcggacgcgc cttgcgaatt gcgctatttg gatgatgaaa ccgccgagct
     2101 ggtgtttgac gaaccgcaat gggcggttac gccgggccag tccgccgtgc tgtacgacgg
     2161 cgacatctgt ttgggcggcg gcatcatcca aacgaccgac aagcctgtca tcatcacacg
     2221 ataaaactta tgccgtctga aacagttttc agacggcatt gttccgctca attccacttt
     2281 aaagaccgat acctatgaaa aaaatttggt tagacagcta cgagaagggc gtcagtgccg
     2341 aaatcgacat cacgcaatac aattccatca gcgacgtatt ccgccaaagc gtggaaaaat
     2401 ttgcccgtct gcccgctttt caaaatatgg gcaaaacgct gacttatgcc gaaaccggca
     2461 aactggcgac tgatttcgcc tcttatctgc aaaacgtcct caagctgccg cgcggcgaac
     2521 gtgttgccat tatgatgccg aacgtattgc aatacccgat agcccttttc ggcattttgc
     2581 aggcaggttt ggtggcggtg aacaccaatc cgctctatac gccgcgcgag ctggagcatc
     2641 agctgaaaga cagcggcgcg accgccatca tcgttttgga aaatttcgcc aacacgctgg
     2701 agctggtgct gccgcgcacg cagatcaaac acgtcatcgt cgcctccgtc ggcgaaatgt
     2761 tcgggctgct taaaggttcg ctgatcaatt tcatcatccg gaaaatcaag aaaatggtgc
     2821 ccgaataccg tattcgggaa accgtttcct ttcagacggc attgaaagag ggagcgaagc
     2881 acgttttcca acctgtcgca ttaaaccgcg aagataccgc gctgctgcaa tacacgggcg
     2941 gcacgacagg tgttgccaaa ggggcggtgc tgagtcacgg caacatctgc gccaatatgc
     3001 ttcaggcaaa agaatggatt aaaaaccagt tgcgtgaggg aaaagaaacc gttatcgccg
     3061 ccttgccgct gtaccatatt tttgccttaa ccgtgaatct gatgattttt gccaatgccg
     3121 gctcgaaaat catcctgatt accaacccgc gcgatatgaa aggctttatc ggacaactga
     3181 aaaaacagcg ggttaatgta tgtatcggcg tgaacacact gtttaacgca atggttaacc
     3241 gtcctgattt tgccgaagtc gatttttcag gattgcggct gactttgggc ggcggtatgg
     3301 cgacccaaaa agccgttgcc gaaaaatgga aaaaaatcac cggcacgccc atcgtcgaag
     3361 cctacggtct gaccgaagcc agccccggcg tgtgctgcaa ccccttaaac atcgaatcat
     3421 acagcggcag catcggtttg cccgtctcgt ccaccgaagt cgaactgcgc gacgcaaacg
     3481 gcaaagaagt ccccgtcggg cagccgggcg aattgtgggt aaaaggccct caagtgatgc
     3541 aaggctactg gaaccgcccc gaagaaaccg ccaaagccat agacgcgcgc ggcttcttgg
     3601 aaaccggcga tattgccgtc atggacgaaa aaggctggtt gaagctggtc gatcgtaaga
     3661 aagacctcgt cgttgtttcc ggattcaatg tttatccgaa cgaaatcgag gaagtcatcg
     3721 cgcatcacgg caaagtcatg gaagtcgcct gtatcggcgt tcccgacgaa aaaaccggag
     3781 aagccctaaa agtattcgtc gtcaaaaaag acccgtcatt gaccaaagaa gaacttatcg
     3841 ccttttgccg caccgaatta accgcatata aagtaccgaa aaatatcgaa ttccgcgacg
     3901 agttgcccaa gtccaacgtc ggcaaaattc tgcgtcgcga gttgcgccaa agtactggga
     3961 aataaagaaa agatgccgtc tgaaaacagc cgtccaccgt tcagacggca tccgcctgtt
     4021 tgccagaacc gcgcgcttca cgttaaaatc acgcattcca atacgggtat ttcattatga
     4081 ccaaattcat tttcgtcacc ggcggcgttg tctcctcact gggtaaaggt atcgccgccg
     4141 cttctattgc cgccatcctc gaatcgcgcg gcttgaacgt taccatgctc aagctcgatc
     4201 cttatatcaa cgtcgatccc ggcacgatga gcccgttcca acacggcgaa gtgttcgtaa
     4261 ccgacgacgg cgcggaaacc gacctcgact tgggacacta cgaacggttt atcgattcca
     4321 cgatgacccg ccgcaacagc ttcagcacgg ggcaggtgta cgaaaacgtc atcgccaaag
     4381 aacgacgggg cgactacctc ggcggcacgg ttcaagtcat cccgcacatt accgacgaaa
     4441 tcaaacgccg catccacgaa ggcgcggcag gttacgatgt ggcgattgtc gaaatcggcg
     4501 gcacggtcgg cgacatcgaa tcgctgccgt ttttggaagc catccgccag atgcgaagcc
     4561 agttgggacg caacaacacc ctgttcgccc acttgagcta cgtcccctac atcgccgctg
     4621 caggcgaaat caaaaccaag ccgacccagc acaccgttaa agaaatgttg agcatcggtt
     4681 tgcaacccga catcctgatt tgccgtatgg acaggacaat gcctgcggac gaacgccgca
     4741 aaatcgcctt gttctgcaac gtggaagaac gcgcgattgt cggcagctac gatgtggaca
     4801 gcatctacga atgccccgaa atgctgcacg accaaggcat cgacaacatc attaccgagc
     4861 agttgcagct taatgtgcaa caggcggatt tgaccgcgtg gaaaaaaatc gtccacgcca
     4921 tccaaaaccc gaaacacacc gtcaaaatcg ccatggtcgg caaatacgtt gatttgaccg
     4981 aatcctacaa atcactgatt gaagccttga aacacgcagg tgttcacacc gaaaccgacg
     5041 tacaaattac ctttgttgac agcgaaagca tcgagaaaaa caacggcgac gtttctatgc
     5101 tcaaagatat ggacgccatc ctcgttcccg gcggttttgg ttcgcgcggc gtggaaggca
     5161 aaatcgccgc tgtgcgctat gcccgcgaaa acaacgtgcc atacttgggc atctgcctcg
     5221 gtatgcagat tgcgctgatc gaatacgccc gcgacgtggc aggtttgaaa ggtgcgaatt
     5281 ccaccgagtt cgacctcaaa tgcgccgccc ccgtcgttgc cctgattgac gaatggcaaa
     5341 ctgccgacgg cagcgtcgaa acccgcgacg aatccgccga tttgggcggc accatgcgtt
     5401 tgggcgcgca agaagtcgaa ttgaaagcag gcagcctcgc cgccaaaatc tacggcagcg
     5461 gacacatccg cgaacgccac cgccaccgct acgaagtcaa caacaactat gttcctcagt
     5521 tggaaaaagc aggcttggtt atcggcggcg tatccgccgg acgcgaacgc ttggtcgaaa
     5581 ccatcgaact gccgaaccat ccttggttct tcgcctgcca gttccacccc gagtttacct
     5641 ccaatccgcg caaagggcat cctttgttca ccgcgtttgt caaagctgcg ttgaacaata
     5701 aaaaagcctg ataaagcgtt acttgatgat caaaatgccg tctgaaagcc tgaatgaaga
     5761 ctttcagacg gcatttttgc aaatcgggga tcataaaagc catcaattta atttttatgg
     5821 ttttatctaa tattgaccaa ctgaatattg ttaagcgggg cgtgaaactc aagttgagac
     5881 ctttgcaaaa ttccccaaaa tcccctaaat tcccaccaag acatttaggg gatttctcat
     5941 gagcaccttc tttcaacaaa ccgcccaagc catgattgcc aaacacatcg accgcttccc
     6001 actattgaag ttggactagg tgattgattg gcagccgatc gaacagtacc tgaatcgtca
     6061 aagaacccat ttaccttaga gaccaccgcg gccgtccgcc tatcc
//
LOCUS       DAWFJR010000108         6019 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWFJR010000108 DAWFJR010000000
VERSION     DAWFJR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.108"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(93..1889)
                     /locus_tag="WGR04_001853"
     CDS             complement(93..1889)
                     /locus_tag="WGR04_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ0795356.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPDDLLDQVWTSRPAIPAETVFIH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSFDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTEQCRLNAEAAAALQTAGIAVEPYAQVAGKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGTNPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            1945..2286
                     /locus_tag="WGR04_001854"
     CDS             1945..2286
                     /locus_tag="WGR04_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795357.1"
                     /translation="MPSEKHNHLIKSPACCKTDGLIGWCVFARKKRCKVVCCKILGIK
                     CRFMPFFGSNMENIVKFEHEVLIVCVGRYLDYVTIRVFIKISLPGGILFLKTVGFGFV
                     WGQSRPNWSNR"
     gene            2347..3600
                     /locus_tag="WGR04_001855"
     CDS             2347..3600
                     /locus_tag="WGR04_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0795358.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCIISATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDKRHKLTAGMNF
                     LAIVGLLYLTGFAVLFLLNLSGLLA"
     gene            3755..5266
                     /gene="lysS"
                     /locus_tag="WGR04_001856"
     CDS             3755..5266
                     /gene="lysS"
                     /locus_tag="WGR04_001856"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ0795359.1"
                     /translation="MSEQNHPQIEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQAVPVKVAGRMMLKRQMGKASFATIQDVTGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASCTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(5543..>6019)
                     /locus_tag="WGR04_001857"
     CDS             complement(5543..>6019)
                     /locus_tag="WGR04_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0795360.1"
                     /translation="TPSKDSDARWIKKNGLYKLGYKQHTRTDAEGYIEKLHITPANAH
                     ECNHLSPLLEGIADGTTVYADKGYDSAENRQHLEEHRLLDGIMRKACRNRPLSEVQTK
                     RNRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
BASE COUNT         1293 a         1555 c         1730 g         1441 t
ORIGIN      
        1 ttgcttgcgg caagatgaaa acggtgcggg atgttttggg aaaccaatgc cgtctgaagg
       61 gctttcagac ggcatttttt gcgccgtgct gtttaacgcg ccagcggttc ggtgcgtttg
      121 atcagccacg cttttgccgc gccttcggtc agcggctcga ggcggcggcg gacttcggcg
      181 tggtagcggt tgacccagtc gatttcgccg tcggtcatga gggcggtgtc catcaggcgg
      241 gtgtcgatgg ggcagagggt cagggtttca aaacagagga agctgccgaa ttcggtttct
      301 tgaggggcgg cgacggcttg gttggcggca aggttttcaa tgcggatgcc ccattttccc
      361 gggcggtaga gtccgggttc gatggaggta accatgcctt ttttcatggc ggtttcgggc
      421 gtggcggggg cggcaaaggc gatgcgttgc gggccttcgt ggacgttgag gaaatagcct
      481 acgccgtggc cggtgccgtg tccgtagtcg cattgcgcct gccacagggg tttgcgacaa
      541 atcgcgtcga tcagcggcga ggggatgttt tcggggaaca cggcttcggc aagcgcgata
      601 tgggctttga gaacgagcgt gttgtcgcgt ttttgttctg ctgtgggcgt accgacgggg
      661 acgacgcggg tgatgtcggt cgtgccgcct ttgtattgcg cgccggagtc gatgagcagc
      721 agcccgttgc cgctgatggt gctgtggctt tcgggtgttg cgctgtaatg cggcagggca
      781 ccgttggcgt tgaagcctgc gatggtgtcg aaactcaatg aaatgaagcc tgggcgcacg
      841 ctgcggtggc gataaagcat ggtgtccacg tcgatttcgg tcaggctgcc gccgttgccg
      901 atgatgtctt caaactcggc gaagaaaccg cacaacgccg cgccgtcgtg ttccatcgct
      961 tcgcggatgc gggcgatgtc ggcttcggat ttgcaggatt tgaacagcgt ggacgggttg
     1021 gtgccttcaa tcaggcgcac gctttcgggc aggcgcacga gcgtgctgac ggcggttttg
     1081 ttcggttcga taagcagcgc gccgccgatt tgcgcgagtt tgccggcaac ttgggcgtaa
     1141 ggctcgaccg cgatgccggc ggtttgcagc gcggcggcgg cttcggcgtt cagacggcat
     1201 tgttcggtaa acaggacggc gttgtctttg ccaatcagca ggaaggacac gaaaacaggg
     1261 ttgaaaggca cgtcgctgcc gcgcaggtta gtcagccagg cgatgtcgtc aaacgaggaa
     1321 accaagtggt aatccgcgcc tttttctgcc atcacggcgc gcacgcgggc gagtttttcg
     1381 gcggcggttt cggagacgta ggcggggtcg tggatgaaca cggtttcggc ggggatagcg
     1441 ggacggcttg tccacacttg gtcaagcaga tcgtccgggt gttcgatgcg gatgtttttg
     1501 gcggtcaggg attgcgccaa agtgcgtttg ccggtgagcg agaccatatc ggaagggatg
     1561 ccgacggcgg cgttttcggg caggtttgcc gcgagccatt cgttatacgg cggcacttgc
     1621 ccgctttttt gcagttcgat gccgctgccc gcaagctgct tggcggcttg ttcccaatag
     1681 cggctgtcca cccatacgcc cgcttcatcg gtggtcagga caaacgtgcc gaccgagccg
     1741 gtaaagcccg acaattcgcg ccgcgcctgc caatgttcgg gcaggtattc ggacaggtgg
     1801 gggtcggcgg aagggatgac gagtgcatcc aagccttgcg ccttcatggc ttcgcgcaat
     1861 gcggacaggt aattcgatac ggtattcata acagttcctc caatcgggtt ttgcggcttc
     1921 agacggcatg gacggaaatc tgaaatgccg tctgaaaaac ataaccatct tatcaaatcg
     1981 ccagcctgct gcaaaacgga cgggctgatc ggatggtgcg tgtttgcgcg aaaaaagcga
     2041 tgtaaggttg tttgttgtaa aattttaggc ataaaatgtc gttttatgcc tttttttgga
     2101 agtaatatgg aaaatatagt caagtttgag catgaagttc taatagtgtg tgttggcagg
     2161 tatttggatt acgttacaat acgtgttttc atcaaaattt ccctaccggg cggtatcctg
     2221 tttttgaaga cggtaggatt tgggtttgtt tgggggcaat cccgcccaaa ttggagcaat
     2281 agatagggct gtgtccgaat atgcggctct gtgtgttgaa acacagttaa aaatacggaa
     2341 gactttatgt ccgaacaaca tatttcgact tggaaaagta aaatcaacgc attgggtccg
     2401 gggatcatga tggcttcggc ggcggtcggc ggttcgcacc tgattgcctc gacgcaggcg
     2461 ggcgcgcttt acggctggca gatcgcgctc atcatcatcc tgaccaacct cttcaaatac
     2521 ccgtttttcc gcttcagcgc gcattacacg ctggacacgg gcaagagcct gattgaaggt
     2581 tatgccgaga aaagccgtgt ttatttgtgg gttttcctga ttttgtgcat catttccgcc
     2641 acgattaacg ctggcgcggt cgccatcgta accgccgcca tcgtcaaaat ggcgattccc
     2701 tcgctgatgt ttgatgccgg cacggttgcc gccttgatta tggcatcctg cctgattatt
     2761 ttggtgagcg gacgttaccg cgctttggat cgcgtttcca aaatcatcat cgttactttg
     2821 agtatcgcca cgcttgccgc cgccggcatc gctatgtcgc gcggtatgca gatgcagccc
     2881 gattttatcg agccgacacc gtggacgctt gccggtttgg gcttcctgat cgcgctgatg
     2941 ggctggatgc ccgcgccgat tgaaatttcc gccatcaatt ctttgtgggt aaccgaaaaa
     3001 caacgcatca atccttccga ataccgcgac gggatttttg atttcaacgt cggttatatc
     3061 gccagtgcgg ttttggcttt ggttttcctt gcactgggcg cgtttgtgca atacggcaac
     3121 ggcgaagcag tgcagatggc gggcggcaaa tatatcgggc aattgatcaa tatgtacgcc
     3181 gttaccatcg gcggctggtc gcgcccgctg gtggcgttta tcgcgtttgc ctgtatgtac
     3241 ggcacgacga ttaccgtcgt ggacggctat gcccgtgcca ttgccgaacc cgtgcgcctg
     3301 ctgcgcggaa aagacaaaac gggcaacgcc gaattctttg cctggaatat ttgggtggcg
     3361 ggcagcggtt tggcggtgat tttttggttt gacggcgtaa tggcgaatct gctcaaattt
     3421 gcgatgattg ccgcctttgt gtccgccccc gtgtttgcct ggctcaacta ccgcctggtc
     3481 aaaggagaca aacggcacaa gcttaccgct ggtatgaatt ttcttgccat tgtcggcttg
     3541 ctctacctga ccggatttgc cgttttgttc ctgttgaacc ttagcggact tttggcatag
     3601 gtttcaaaat ctgaatgccg tctgtaatat tttcagacgg cattgccctt tcctagaaaa
     3661 aaacaccgac atcgcgtaaa atatgcacaa attttgtgat tcaatcaggt cgtctgaaac
     3721 agattgcgcc tgatttattt tttcggaaaa taccatgagc gaacaaaacc atccgcaaat
     3781 cgagccgcag ttggacgaaa accaaattat cgccctgcgc cgtgaaaaac tgcacaacat
     3841 ccgccaacag cgcaacgcct accctaacga tttcaaacgc gacagctttg ccgccgattt
     3901 gcacgcccaa tacggcgaaa tcggcaaaga agaactcgac ccgcaggccg ttcccgtcaa
     3961 agtggccggc cgcatgatgc tgaagcgtca aatgggcaaa gccagcttcg ccaccattca
     4021 agacgttacc ggtcaaatcc agctttatct gaacaacaaa ggcgtgagtc aggaagtttt
     4081 ggacgatttc aaccattggg atttgggcga tatcgtcggc gcggaaggta ctttgttcaa
     4141 aaccaaccac ggcgaattga ccgtgcgcgt gtccgacatc cgcctgttgt ccaaatcctt
     4201 gcgtccgctg ccggacaaac acaaaggctt gagcgatcag gaaaccaaat accgccaacg
     4261 ctatgtcgat ttgattgcca acgaagaatc gcgcaatacc tttatcaaac gcagccaaat
     4321 catccaatcc gtgcgtaatt ttatggtggg cgagcattat ctcgaagtcg aaaccccgat
     4381 gatgcacccg attcccggcg gcgcgacggc aaaacccttc gttactcatc acaatgcctt
     4441 agatattccg ctttacctgc gtatcgcgcc tgagctgtat ttgaaacgcc tggttgtcgg
     4501 cggcttggaa cgcgtgtttg aaatcaaccg cagcttccgc aacgaaggca tgtccgtgcg
     4561 ccacaacccc gaatttacga tgattgaatt ttacgaagcc ttctccgact acgaacgtat
     4621 gatgcagatg gcagaagaca tcatcagaaa cgcatcgtgc acagtaaacg gtacggcaaa
     4681 catcacttac aacggcaaag aagtcgattt ggaaagcccg tttgagcgtc tgaccatcct
     4741 cgaagccatc aaaaaataca atccgcacta caccgacgag cagttgaacg atgcggaatg
     4801 gctgaaaaaa gaaatcgtca aacacggcga aagcctgccg ccgtccccgg gcatcggcag
     4861 cctgcaactc gcgctgtttg aaggttgcgc cgagggcaag ctgtggaacc cgactttcat
     4921 catcgattac ccggtcgaag tttccccatt ggcgcgcgct tcggatacca aacaaggtct
     4981 gaccgaacgt ttcgaattgt tcgttgtcgg ccgcgaactg gcaaacggct attccgagtt
     5041 gaacgacccc gaagaccaag ccgaacgctt caaagcgcaa gtggcgcaaa aagacgcggg
     5101 cgacgacgaa gcgatgcact acgatgccga ctacatccgc gcgatggagt tcggtctgcc
     5161 gccgacaggc ggttgcggca tcggtatcga ccgcttggta atgctgctga ccgattcgca
     5221 aaccatccgc gatgtgattc tgtttccgca gatgcgcccc gagtaatcat aaaaacagtt
     5281 gaaatggcaa tgccgtctga acccgattgg attcagacgg cattttgtat ggcaggattg
     5341 atagtgaatt aacaaaaatc aggacaaggc ggcgagccgc agacagtaca aatagagacc
     5401 tttgcaaaat tcctttccct cccgacagcc gaaaccccaa cacaggtttt cggctgtttt
     5461 cgccccaaat accgcctaat tttacccaaa taccccctga atcctccccg gatacccgat
     5521 aatcaggcat ccgagccgcc ttttaggcgg cagcaggcgc acttagcctg ttggcggctt
     5581 tcaacaggtt caaacacatc gccttcagat ggctttgcgc actcacttta atcagtccga
     5641 aataggctgc ccgggcatag cggaatttac ggtgcagcgt accgaagctt tgttcgacca
     5701 cataacggtt gcgtttggtt tgcacttccg acagcggacg gttgcggcag gctttgcgca
     5761 taatgccgtc cagcagccga tgctcttcca gatgttgccg gttttccgca ctgtcatagc
     5821 ctttgtcggc atagacggtc gtaccgtcgg ctatcccttc cagcaaaggc gacaggtggt
     5881 tgcactcatg ggcattggcg ggggtaatgt gcagtttctc gatatagcct tctgcatcgg
     5941 tacgtgtatg ttgtttgtaa ccgagtttgt agaggccgtt tttcttgatc caacgggcat
     6001 cgctgtcctt actcggtgt
//
LOCUS       DAWFJR010000109         5875 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWFJR010000109 DAWFJR010000000
VERSION     DAWFJR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.109"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(613..3351)
                     /gene="tbpA"
                     /locus_tag="WGR04_001858"
     CDS             complement(613..3351)
                     /gene="tbpA"
                     /locus_tag="WGR04_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ0795361.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDTNRYANFIVEEECENRNYETCKANP
                     KKEVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHT
                     QQTFDTRDMTVPAFLTKAVFDENRKYGPIRGNGKYAGNHKYGGLFTNGENGALVGAEY
                     GTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGS
                     DKNCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGSN
                     LRHQDYYYQNAKRAYSLKTPPQSGKNSPNGDKSKPYWVSIGGGNVVTGQICLFGNNTY
                     TDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTLSWN
                     AGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGIVFK
                     GDFGNLEASWFNNAYRDLIVRGYEAQIKNGKEEAKGDPAYLNAQSARITGINILGKID
                     WNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYDQPE
                     GKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTIKKH
                     FTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF"
     gene            complement(3437..5602)
                     /locus_tag="WGR04_001859"
     CDS             complement(3437..5602)
                     /locus_tag="WGR04_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0795362.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPQAQKDQGGYGFAMRLKRRNRYPTAMPKENEVKLKNDDWEATGLPTEPKKL
                     PLKQESVISNVQTDNGDNNIYISPYLTQSSHNSHNGSINGGANQPKNEATGYNNFKYV
                     YSGWFYKHAASERKFNEGKFKSGDDGYIFYHGKDPSRQLPASGKVTYKGVWHFVTDTK
                     LGQKFNDIIQPSKKQGDSYSGFSGDEGEEYSNKNESTLKDGHEGYGFTSNLEVDFGNK
                     KLTGKLIRNNASQSNTNNDKPTTQYYSLEAQVTGNRFNGKAIATDKPQENETKQHPFV
                     SDSSSLSGGFFGPQGEELGFRFLSNDQKVAVVGSAKTQDKPRNGAVASGGTGAAASDG
                     AAGTSSKNGKLTTVLDAVELTHGGTAIKNLDNFSNAAQLVVDGIMIPLLPEASESGKN
                     QANQGTNGGTAFTYKTTYTLESDKKDTKAQTGAGGAQAASGAAGVNGGQAGTKTYEVE
                     VCCSNLNYLKYGMLTRKNSKSAMQAGENGSQADAKTEQVEQSMFLQGERTDEKEIPKE
                     QQDIVYRGSWYGHIANNTSTSWSGNASNATSGNRADFTVNFGDKKITGTLTANDRTQP
                     TFTITANIKDNGFEGTAKTAELGFDLDQSNTTGTPKAYITNAKVQGGFYGPKAEELGG
                     WFAYSGDKQTENTTVASGNGNSASSATVVFGAKRQQPVQ"
BASE COUNT         1254 a         1480 c         1454 g         1687 t
ORIGIN      
        1 aggtttctgc ttttgatttt ctgttttcgc gggaataacg gtttagaagt tacccgaaac
       61 ctcaaaaaaa cctaaaccga acggactgga ttcccgcctg cgcgggaatg acgaatttca
      121 agattacggt attgtcgggg atgatggaaa acggtggaaa ttgtgtaaaa aatgccgtct
      181 gaagcctttc agacggcatt tgcggcattc ggacatttaa aaaccccatc attcccgcgc
      241 aggagggaat ccgaatacat ccgaacagaa acctgcatcc cgtcattccc gcgaaagcgg
      301 gaatctagaa cgtaaaatct aaagaaaccg ttttatccga taagtttccg caccgacagg
      361 tctagattcc cactttcgcg ggaatgacgg gattttagat ttctgttttt gattttccgc
      421 tttcgagaaa ataacggttt agaagttacc cgaaacctca aaaaaaccga aaccgaacgg
      481 actggattcc cgcctgcgcg ggaatgacgg gattcgagat tacggtattg tcggggacga
      541 tgggaaatgg tgggaattgt gtaaaaaatg ccgtctgaag cctttcagac ggcatttgcg
      601 gcgtttggac gtttaaaact tcatttccaa gctaaatgtg tagttgcggc cgggggcggc
      661 atatcggttg taaacgccga catttttgtg ttggttgact gcgccgccgg cagtttgccg
      721 cacattttcc caagtaacat agcggtagtt gaggaggttg tacacgcccg cacggagggt
      781 gaagtgtttt ttaatcgtgt aataaccgga cacatccaca atataccaag ggcgggtacg
      841 gcgcgcggtg gcttttgtat tgcggctgtt gccgttgagc aaagcccggc tgcccaacaa
      901 ctctgtgatt tccttggctt tggaataagt cagcataccg ttcacacccc atttgccttc
      961 cggttggtca tagcccaagc cgacgacata gcgcgagggt tggatggcat caaacaggtg
     1021 tgattgaata tcggtgcggt ctgcgcgttt tttgatgtcg cggacacgga cacgattata
     1081 ggcaaatgta gaataccaac cttcgggcaa tttatcccat acgccgttcc aatcgatttt
     1141 gcccaaaata ttgatgccgg taatccgcgc gctttgggca ttgaggtaag ccgggtcgcc
     1201 tttggcttct tctttgccgt ttttaatttg cgcttcataa ccccggacaa tcaaatcgcg
     1261 gtaggcattg ttgaaccaac ttgcctccaa gttgccgaaa tcgcctttaa acacgatgcc
     1321 ggcttctttg ttgaacgatt tttccggatc gattttgacc gccttgcttt gaacacccgc
     1381 ccgccagccg tacatttccg caaacgaagg caggcggaag ccggttgaag tgcggtaagt
     1441 caaatccagc cagtcggcag gtttgaggac gatgccggcg ttccaggaca gggtgcggtg
     1501 cgtgccggtg gaaacgctgc cgtcgtccga atgcgtgctg cggtagtcgt agcgcaaccc
     1561 cgcaccgaca tccgcccacc tgcccaaacg gacattgtcc cgaactgctg cgtaatagct
     1621 tttgccgttg atgctgcgcg gcgtgcagtc cgtataagta ttgttgccaa agaggcagat
     1681 ttgccccgta accacatttc ccccgcctat gctgacccaa tagggtttgc tcttgtcgcc
     1741 gttggggctg tttttgccgc tttgaggggg cgttttcaac gaataggcgc gtttggcatt
     1801 ttgataataa taatcctgat ggcggagatt agagccaaag cggtcaaacc cgagattcac
     1861 gctcaggttg tggcggattt tggcggtatc gaaggatttt ttgaatgccg cctgcaagag
     1921 cctgtggctt tccccgtaaa tcacgcgatc ggatttgtag taggaaaacg gcttgtcggc
     1981 actcggacgg caatttttgt ccgaaccgtc ggcagaacag tgcgtctgct gaaaatgatt
     2041 gtccaaaccg ataccctgcc ggtcataaga gaggcgggca taatccgccc aagtgtcttt
     2101 atcggcattg gtatagacat attccaaacc gtagcggctt ttggtgtgcg tctcgtcgta
     2161 aaacacgcct gtaccgtatt ccgcgcccac cagcgcaccg ttttcgccgt tggtaaacag
     2221 tccgccgtat ttgtggttgc ccgcatattt gccgttgcct cttatcggtc cgtattttct
     2281 attttcatca aaaaccgcct tggtcaggaa tgccggaacc gtcatatcgc gcgtgtcgaa
     2341 agtttgttgc gtgtgttcga gtatgccgcc gatgtagtgc cgtttgtttt caaaacgaaa
     2401 acccgggcgg aacaaccacg atcggctttc gtatgaaagc ggatcggcga ggaagcggtt
     2461 gggacccgtg tagtctcggg tggaaaccgt ttgccgttcg tctttgccga caacctcttt
     2521 tttcggattc gctttacacg tttcgtaatt tctgttttcg cattcttcct caacgatgaa
     2581 atttgcgtac cgattggtat cctcaaccgg caccagcctg ttaaagctct gaacgccgcg
     2641 tccggcatct tcgtgtgcgc ggatttcccc cgcgcgccgc ccggtgtgga tcagcaaagc
     2701 ctccgcaccg ccgatgcgcc ccgccagcgc gatggattgg gtaagccccc ggtttttgcc
     2761 ggaataggcg gttttactct gaatgcccca ctgcctgcct tccccgataa catcgtcggc
     2821 ggttttggtt tgaaatgcga ccgagcccgc caatgcgccg ctgccttgtt cgaccgagtt
     2881 tgagcctttg ctgatttcga cagctttgac gttttcatac tcgatttcat tgattgcgcc
     2941 gctgctgccc gccgtcctcg tcccgcccaa tgccgcctgc gcggtgtagg actgtatttg
     3001 cgccaagccg tccaccgtca aggaaacgcg gtttttgtcc ataccgcgta tcgagtagcc
     3061 agagcttgcg ccgcgcccct gttcgaccac ggcgatgccg gggtcgtaac gcgtcaggtc
     3121 gcggatatcg agtacctgtt ccttgctgag ggtgtcggcg gttttgacca atttgcccag
     3181 accggttact tcgttatcgc ggcgggtttt ctgttttttg gcttttacct gtatggtatc
     3241 caactgtttt tcctgtgctt gtccggcttg cacattttct gcataaacgg gcagcgcagt
     3301 catcagcgac aggcataaaa tatttaatcg gaacaaatgt tgctgttgca taatgtttcc
     3361 ctaatcttcg ctttcagacg gcatcggaag gagcgatgcc gtcgaagcct tattcttgat
     3421 tgttcggcaa ccgtgcttat tgcacaggct gttggcgttt cgcaccgaat acgacagttg
     3481 cactgcttgc tgaatttcca ttgccggatg caactgttgt attttccgtt tgtttatcgc
     3541 ccgaataggc aaaccatccg cccaactctt cggctttagg tccgtaaaaa ccgccctgca
     3601 ccttggcgtt tgtgatatat gccttaggcg tgccggtggt attgctttga tcgagatcaa
     3661 aacctaattc agcagttttt gccgtacctt caaagccgtt gtccttaata ttagccgtga
     3721 tagtaaaggt tggctgagtc ctatcattag cggttaacgt gccggtaatt tttttatcgc
     3781 caaaattcac ggtaaagtcc gccctgttgc cactcgttgc attggaagca ttgccgctcc
     3841 agcttgtgct tgtgttgttg gcaatatgcc cgtaccaaga cccccgataa acgatgtctt
     3901 gttgctcttt tggaatctct ttttcatcgg tgcgctcgcc ttggaggaac atactttgtt
     3961 caacttgttc cgttttagcg tcagcttgac taccgttttc gcctgcctgc atcgcggact
     4021 tgctgttttt acgcgtcaac attccgtatt tcagataatt gaggttggaa cagcagactt
     4081 caacttcata ggtttttgtt cctgcctgcc cgccgttaac gcccgccgca cccgaagcag
     4141 cttgcgcgcc gcccgcgcct gtttgggctt tggtgtcttt tttatcactt tccagcgtgt
     4201 aggttgtttt gtaggtaaag gctgttccgc cgtttgtacc ttgattggct tgatttttcc
     4261 cactttcgga agcctcgggc aggagcggaa tcataatgcc gtcgacaacc agttgggcgg
     4321 cattgctgaa gttgtcgaga tttttgattg ctgtgccgcc gtgcgtcagc tcgaccgcat
     4381 ccaaaaccgt ggtcagctta ccgtttttag acgacgtgcc tgccgcaccg tccgatgctg
     4441 ccgcacctgt gccgcctgaa gccactgcgc catttctcgg tttgtcttgg gttttcgcgc
     4501 tgccgacaac ggcaactttt tgatcgttgc tcaaaaagcg gaaacccaat tcctcaccct
     4561 gcgggccgaa aaagccgccg ctcaaagaag acgagtcgga aacaaaggga tgttgtttgg
     4621 tttcattctc ttgtggtttg tcggtcgcta ttgccttgcc attgaagcgg ttgcctgtta
     4681 cttgagcctc aaggctgtag tattgcgtgg taggtttgtc gttattagta ttactttggc
     4741 tcgcattatt gcgtatcaat ttacccgtca atttcttatt gccgaaatcc acttctaaat
     4801 tcgaggtaaa tccataaccc tcgtgaccat ctttcagcgt ggattcgttt ttgttggaat
     4861 attcttcgcc ctcatcacca gaaaatccgc tgtagctgtc gccttgcttt tttgaaggct
     4921 ggataatatc gttaaatttt tgtcccagtt tcgtatcggt tacaaaatgc catacgccct
     4981 tgtaggtaac ttttccagaa gcaggaagtt gtcgggaagg gtctttaccg tgataaaaga
     5041 tatagccgtc gtcgcctgac ttaaattttc cttcattgaa ttttctttca ctcgccgcat
     5101 gtttataaaa ccaaccggaa taaacatatt tgaaattgtt gtaacctgtt gcttcgtttt
     5161 ttggttggtt tgcaccgcca ttgatactgc cgttgtgaga attatggctt gattgcgtga
     5221 gataagggga aatgtaaata ttgttgtcgc cattatctgt ttgtacgttt gaaatgacgg
     5281 attcttgttt taatggcagt ttcttgggtt ctgtcggcaa tcctgtcgcc tcccaatcat
     5341 cattctttaa tttaacctca ttttctttag gcattgccgt aggataccga ttcctccgtt
     5401 tcaacctcat cgcaaaaccg tatccgcctt ggtctttttg ggcttgcggt ttttcggaag
     5461 aaacatcttg atactttggc gcgggacgcg gggcttcggt atcgacagaa tcaagatcga
     5521 agctgccgcc gccgcccaaa caagcactca acaaaaacac aggcagcacc atagcagcct
     5581 gatttaccaa tggattgttc atataaaccc aattcaatta aagaatgata aggattatta
     5641 ttttatttat ttttaacaaa tttgcaaata cttttttatt tttttaggga atacaccaaa
     5701 atcccgtcat tcccgcgcag gcgggaatcc gaacgcgtcc gcacggaaac ctatatcccg
     5761 tcattcccac gtatatcccg tcattcccgc gcaggcggga atccagtgcg ttgagtttca
     5821 gctatttaga ataaattttg aaactctaat cgcgtcattc ccacgaaagt gggaa
//
LOCUS       DAWFJR010000110         5714 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWFJR010000110 DAWFJR010000000
VERSION     DAWFJR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.110"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(231..1295)
                     /locus_tag="WGR04_001860"
     CDS             complement(231..1295)
                     /locus_tag="WGR04_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0795363.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKAKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPETKNALATAGH
                     QCVANRFTINHTADKLLDAI"
     gene            complement(1296..2054)
                     /locus_tag="WGR04_001861"
     CDS             complement(1296..2054)
                     /locus_tag="WGR04_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ0795364.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(2129..2204)
                     /locus_tag="WGR04_001862"
     tRNA            complement(2129..2204)
                     /locus_tag="WGR04_001862"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2169..2171),aa:Ile,seq:cat)
     gene            complement(2258..3394)
                     /locus_tag="WGR04_001863"
                     /pseudo
     CDS             complement(2258..3394)
                     /locus_tag="WGR04_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
     gene            complement(3339..3935)
                     /locus_tag="WGR04_001864"
     CDS             complement(3339..3935)
                     /locus_tag="WGR04_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0795365.1"
                     /translation="MKITHCKLKKKVQKELLHFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLVLAADEVFEDPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVNNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLDVSSFIHYRINHSKEFADRQ
                     NHINGIENFWNQAKRVLRKYSGLARTFPRIGSATGCAD"
     gene            3982..4551
                     /locus_tag="WGR04_001865"
                     /pseudo
     CDS             3982..4551
                     /locus_tag="WGR04_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691695.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            4783..5283
                     /locus_tag="WGR04_001866"
     CDS             4783..5283
                     /locus_tag="WGR04_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795366.1"
                     /translation="MVFVNPLYYGREAGWFVWLAAAMCGLWLLRALTAKLPQQRYAAL
                     FITLFFAAILVFGRHDSMYWYPVLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEK
                     AVRYTRRVTQIWCVFFIINGTLAALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYR
                     KLVLKV"
     gene            5290..5571
                     /locus_tag="WGR04_001867"
                     /pseudo
     CDS             5290..5571
                     /locus_tag="WGR04_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
BASE COUNT         1294 a         1352 c         1539 g         1529 t
ORIGIN      
        1 gtactggttt ttgttagtct actatacatt gtaacaaagc acttgatgcg gcagcacaag
       61 ccggggaaga cgctgcaaaa ctttgccaat aggctgcgta atatcggggg atattgggat
      121 tttcttattt ttaaggatgg attcggggtg tttctttcca atgaacgaat ataatttggc
      181 ggtttgttgc atcaaaggct ttcagacggc atatgccgtc tgaaagcagt ttatattgca
      241 tccaataatt tgtcggcggt atggttgatg gtaaaacggt tggcgacgca ttgatgtcct
      301 gccgttgcca gtgcgttttt cgtttcaggg ttcaagacga ggcgttcgat ttcgtccgcc
      361 aaagattcag gtgtcagcct gtccagcaga atccccgatt gatgatgttc gataatttcc
      421 ttttgcgccc ccaaagtatt ggaaatcacc gccgttcggc agtacatcgc ctcgcataat
      481 gacaaaccga atgcctccgg gacgaggctg ggcaaaacca cgacatcgct ttggcggtaa
      541 aacgaagcaa gtttttcggt aaacccttca aaagaaacaa atggttctgc tcctgaagca
      601 gatacgtccc gcttcaggcg gcacatataa tccggatgtc cgtcccctgc caatttgagc
      661 ctgatttgag gatatttccg atgcagtatc acacaggctt caatcaggtt ttccaatcct
      721 ttttctggac tgatcctgcc ggcgtaggcg acggtaaaaa aacggctgtc gggtttttct
      781 tgagagggag ggaaacggcc ggtatcgata ccgttatgaa caatccggta ttttgcttta
      841 aagggattgt cggcggtttg cacatcgtaa accagacggg aaacgcaaat aaagcggtct
      901 gtgtttttct gtatcaggcg gtggtaaaaa tcggttttgt tggcgacgac attatgtttg
      961 acaaatatca ggcgcacccc ggtcagtttt ttcaaaagta tggataaggc ggcaattttg
     1021 ccggtgtgta tcatcaggtg ggaaatgcgg tttttccgga taaagcgggt aagcgcgcag
     1081 agcgaaaaca gcccgttgag cgtgtgaagg cgcgttgtga aaacggaaga aacttcggaa
     1141 aaacgtctgt gcatcaactc attattttta ttgacggcgg taaacatcgt gcaaccccga
     1201 agccccaatg cttttgaaac atcatagaca tactgttcgc ccccgcccca aatcttggcc
     1261 gatacgatat ttaatatcct gaattctttt tccatttaaa attttccgcc agatttgtac
     1321 agataatata gtttgacata tttaatcatc gtgtaatagc tgtggttgac ggacataatc
     1381 caacccattt ttccatcgag aaaccctttg ttcaggatat aaattttgaa aaaaccccaa
     1441 atcgggcgga ggataatgtc cctaacgaaa cgcacgggct ttccctgctc tcggtatttc
     1501 tcggctgaga tggaagtgta tttgttgaat ttgttgaaat attgttccca gttgtcgtat
     1561 gtgtaatggt acataaaatg cttcagacgg cgtttggggt agggggtttg tacggtttcg
     1621 tgtactttgc cttgcaccga actgtctttt ttcggcatca gacggcatac gctgtcggga
     1681 cgcatcgcgc cgtgtgtggc gggatggttg gggaaaaggt tgcggcgttc gacaaaatag
     1741 gcggcataat cgccggtttg gacaattttt gagatttcat cagatagttc cggcgtgcag
     1801 cgttcgtctg catcaatcag gaaaacccat tctccgcctg cctgttcgat ggcaaatgtt
     1861 ttttgcgctc cgaaatcccc gttcaaatgc cgtctgaaga cttttgcacc caaaccctcg
     1921 gcaatttcgg cagtattgtc ggtgctgtgg tcgtcaataa cgataacttc tttatcgaaa
     1981 cggcaacttt caatacattc ccgaatgtgg tttgcttcgt ttttggcaac aatcaatacg
     2041 ctgacttttt tcattatcaa ggtttctcaa agcattttgg tttcacaggg cggcaaaaaa
     2101 gcccaaacat acgtccgggc ttgggaattg gtgggtccgg tggggttcga acccacgacc
     2161 aagggattat gagtcccctg ctctaacccc tgagctacag acccgtcaag agtaggcgcg
     2221 tattgtatag catagcggtg ggattgacaa acttatttca tccgatgatt aagacgcagt
     2281 tgcttcctcc gaaggcaaac gacgagctgg cggcaatgtg tttttcggtt tcccagcggc
     2341 tgccgctgcc ggtcaggttg atggcgggca ggtcggggtc gttctgcccg tcccaaagcc
     2401 ggggcggaag tttcccttcg ggattgcttt gccggtcggc aatgccccac gcgaacgcgg
     2461 cttcgattgc gcccgccgcg cccagcgtgt gtccggtttg cggcttggtg gacgtgcagg
     2521 gcgtattgtt gccgaaaacc gctgcaacgg cacggctttc catactgtcg ttgtggtgcg
     2581 tcccggtgcc gtgcagatta atccagccga tgtcttcggg cgcaagacct gcgtgttgca
     2641 atgccgtctg aaaggcaagg attgcgcctt gcgcgtcggg gcggggcgtg gaaatatggt
     2701 aggcatcgct gttggctccg tagccgacga gttcggcgta aattatcgca ccgcgccgtt
     2761 tggcgtgttc caattcttcc agcacgaaaa tccccgcgcc ttcgccgatg accagcccgt
     2821 cgcggttcgc gtcgtatggg cgcggggttt tttccggttc gccgttgcgg cggctggcgg
     2881 cataaagcga gtcgaaaaca tacacttcgg acgagaaaaa ttcttcgcct ccgcccgcca
     2941 gcatcatatc ggtcagaccg tatttgatgg cttcgtaggc ataacctatg ccttggctgc
     3001 cggacgaaca cgcgctcgat gtcgggatga tgcgtccttt cagcccgaaa aagatgccga
     3061 tattggcggc ggtggtgtgc ggcatcatac gcacataggt gttggcactg aagttgcgcg
     3121 acgtgccggt cagcaacaat tcgcccacat cgccgatgtc tttggtgctg ccgctggaag
     3181 agccgcaggc aacgcccatc cgtccgtcgg taatgctttc gtccccgagc aaaccggcat
     3241 ccgtcaaagc ctgctccgcc gcatcgacgc acaaatacga cacacgcccc atacttctga
     3301 gctgcttgcg cgtccaatgt ttcggcggcg cgtaatcctc aatcggcgca cccagttgcg
     3361 cttccaattc ggggaaacgt tcgtgccaat ccactatatt ttcgcaagac gcgttttgcc
     3421 tgattccaaa agttctcaat gccgttaatg tggttttgac ggtctgcaaa ttccttggaa
     3481 tggttgatgc ggtaatggat aaaactgctc acgtccaact tgtcgcagct gctcagacta
     3541 tcggtataaa caatactgtc cggcatgatt ttctttttga tgacagggag taacgtttca
     3601 gacttggcat tgtttacgac aacggtatag acccgtccgt tgcgtttcag aatgccgaaa
     3661 acaaccactt ttcctgccgc accgcgacca cgtctgcctt tacgccgtcc gccgaaatcg
     3721 ctttcgtccg gctcgacagg atcctcaaaa acctcatcgg cagccaagac caaatgatgg
     3781 ttgataaccg tgcggatttt acggtagaac agtgctgccg aattgggatg gatacccaaa
     3841 atatcggcgg cagaacgggc ggtaacttcc agtacaaaaa aatggagcag ttctttctgt
     3901 acttttttct ttaatttgca gtgtgttatc ttcatatttc gagggtaaca tatctgctaa
     3961 tctagtaaaa tttaatccac tatagtttat gcctcgcacg acggggaaat ggcgcgcagt
     4021 ttcgatttgt ggttggaact gttgaaatcg caaatcgtgt cgccgacttc gttcggtttg
     4081 tcggtgcaca atgcaacggc ggggtaatgg tcgatattga ggcgggatat gagcgagcag
     4141 acggcattgg ccgtgtgtgc cgacggtgtg gaaacggcat tggcggaagc ggcatcactg
     4201 ttggaagagg gatgcgggtc ggttttggtt ttggcggcgg acgatccgct gccggaagga
     4261 tatgccgtat cggcaacgcg cgcgccgttt gcttatgctt tggcgatggt tctgaccaaa
     4321 gggacgcgtt acagcctgac tttatctgct tcggacgata tgccgtctga agccggtatg
     4381 ctgcccgagg catattggag tggtttggaa tgggtgcgct ttctgctgaa cggcagtcgg
     4441 gaatgccgcc gggtatatcg caatcgtgaa tggctgtggc agcccgcaag ctgcaggctg
     4501 aagatttccg caatgtccgc accgtcaacg acgtggtaca ggcggtattg aagattcagg
     4561 cgggctaatc gggtatttct aattattttt cagacggcat gtttttatcg gatgccgtct
     4621 gaaaacattt gtgcggattc aaaatgaaac ccggtttttt cggacatatt tttctgaccg
     4681 tattaagcat tgcctatccc gctttgtggt atagtggatt aacaaaaatc aggacaaggc
     4741 gacgaagccg cagatagtac ggcaaggcga ggcaacgctg tactggtttt tgttaatcca
     4801 ctatattacg gcagggaggc gggctggttt gtctggctgg cggcggcgat gtgcgggttg
     4861 tggctgctgc gcgccctgac tgccaaactg ccccaacagc gttatgcggc tttgtttatt
     4921 acactgtttt ttgccgcaat attggtgttc gggcggcatg attcgatgta ttggtatccc
     4981 gtcctcgtca atctgatgat gttggcggtg ttcgggggaa gcctgtttgc cgggcagacg
     5041 gtgattgaaa aacttgcgcg gctgcaacag cccgacctgc ctgaaaaagc cgtgcgctat
     5101 acccgccggg taacgcagat ttggtgcgta ttttttataa tcaacgggac gcttgccgct
     5161 ttgttggcac ggttggggcg atatgattgg tgggcggttt atacaggcgt gattgcctat
     5221 gtattgatgg ggatgctgtt tgccggcgaa tggctttacc gcaaactcgt tttgaaagtc
     5281 tgaaacctta tgtacacttg cacttcctgc ggcgaaaaac atgaagatat gcccgcaatc
     5341 ggctttaccg ccccctatcc ctataatcaa ctcagtgaag aagaacgcat tgcttatcaa
     5401 gcggaatgcg attctgattt ctgcatcatc cgctatcccg accaaaccga ccgttttatc
     5461 cgcgcggtgc tgccgattcc gattatcggg catcaggaaa cgctggaatg cggggtgtgg
     5521 gtatctgtca gagaaaagag ttttaacgac tatcaaagcc gcttttacaa ttatcgtaat
     5581 ggatgaagga aatatggcag ccctcagaat ctctttactg atccacaaac tgatgttact
     5641 cgtcgcttag tcgaaagcta ttttggcaaa attttagatg aaactgcttg ggtaaaagac
     5701 aaaaacactc acta
//
LOCUS       DAWFJR010000111         5691 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWFJR010000111 DAWFJR010000000
VERSION     DAWFJR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.111"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            98..814
                     /locus_tag="WGR04_001868"
     CDS             98..814
                     /locus_tag="WGR04_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ0795367.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILAGLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            963..1865
                     /locus_tag="WGR04_001869"
     CDS             963..1865
                     /locus_tag="WGR04_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ0795368.1"
                     /translation="MDSLMTLLSVLVPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASTDGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIRGAGGLEAVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            1908..2681
                     /locus_tag="WGR04_001870"
     CDS             1908..2681
                     /locus_tag="WGR04_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ0795369.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EDAGSLKRHHGGASSGGNLPEGLPADRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVVSELVRRRGSLRVITNNIHAASIASARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2793..4481
                     /locus_tag="WGR04_001871"
     CDS             2793..4481
                     /locus_tag="WGR04_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ0795370.1"
                     /translation="MLNKDQFADNHFIRTIIEEDLESGKHTAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAYHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFEAGKTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4574..5530
                     /gene="gshB"
                     /locus_tag="WGR04_001872"
     CDS             4574..5530
                     /gene="gshB"
                     /locus_tag="WGR04_001872"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ0795371.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSSGLSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
BASE COUNT         1324 a         1547 c         1576 g         1244 t
ORIGIN      
        1 aatttgtgca atgctttaat tcctctgaca cgatttgaaa cacagccgcc ttaaaacatc
       61 ttaaaaaatt tgaaagcgta aaaatacgga aaacatcatg aactttgaaa acgacgacat
      121 tatccatgcg ccgaccacgt cttccctgat tctcgaagag cggcacgatt cggagctgtt
      181 ccgtgtttac gccctgattt tggacggcat taccgatcag gtgctgctgc ccggcaaaaa
      241 gctgaccgaa tccgaacttt gccgccagat ggtgtgttcg cgcaacaccg tccgcggcgc
      301 gctgtcgctt ttggcgcacg acaagattgt cgatttgcaa cccaacaggg gcgcgttcgt
      361 ccacgttccc gatttgaaag aaatgcagga tgtgttcaat gcgcgcatcg aaatggagac
      421 gatgattttg aacatcctcg ccggcctgcc ggatttggaa acgcgcctca agccgcttta
      481 tgcgatgata cggcgcgaag aagaggcttc cggcaggggc gaccgcgtcg gctggaaccg
      541 cctgtccaat gccttccacg tcgaactggc gcgcctggtg ggcaacgatg tgctgttcga
      601 cattatgaat acgctgtgcg cgcggtcatc cctgattgtc gccgtggcgg gcgtgcatcg
      661 cgaggaaaaa cacgccatca atacgcatac gcattccgaa caccgcgaaa tcctcgacct
      721 gctgctggcg ggcaggcgca accgcgtggt caaaatcctg cgccgccatt tgggcaactg
      781 tatggagcgt ttggaaaaga ctttggaaga ttgaatgccg gagcggataa gccgcctgaa
      841 ccttcaggcg gttttttaat ggcgaacctg atgccgtctg aaatatggat gcgggtatct
      901 gcaattttca gacggcaatt tttaaaccgc atatatcatg cggcaataaa ggagggcagt
      961 agatggacag cctgatgaca ttgctttcgg tattggtacc gatgttcgcc ggatttttta
     1021 tccgtgtgcc caagccttac ctgcccgctt tggacaaggt gctgtcggtt ttggtgtatg
     1081 ccgtgctgct gctgatcggc gtatcgttgt cgcgcgtgga ggatttgggt tcgcggttgg
     1141 acgatatggc gttgacggtt ctgtggctgt ttgtttgtac ggtcggggcg aacctgcttg
     1201 ctttggcagt gttgggaaag ttattcccgt ggcggataaa ggggaaaggg aagggcgttt
     1261 cggtcggcgt gtcgggcagt gtggggcagc tcggatgcgt gctgctcgga tttgcatccg
     1321 gcaaactgat gcgcgatatt tggatgccgt ctgaaagcgc gggcatgtat tgtctgatgc
     1381 tgctggtgtt cctcatcggc gtacagctca aaagcagcgg cgtatcgttg cggcaggttt
     1441 tggtcaaccg caggggtatt cggttgtcgg tctggtttat gctttcatct ctttcgggcg
     1501 ggctgctgtt tgccgcatcg acagacggtg tgtcgtggac gaaaggtttg gcgatggctt
     1561 ccggcttcgg ttggtattcc ctctcgggtt tggtcatgac cgaggcttac ggcgcggtat
     1621 ggggcagcat catgctgctg aacgatttgg cacgagagct gtttgcactg gcatttatcc
     1681 cgctgctgat gaagcgtttt cccgatgcgg cagtgggggt cggcggcgcg accagtatgg
     1741 atttcacatt gcccgtgatt cggggtgcgg gcggcttgga agccgtaccg gtagcggtca
     1801 gcttcggcgt ggtggtcaat atcgccgctc cgtttctgat ggtggtgttt tccgctttgg
     1861 gctgaacgcg gtaaaatcgg catcccgatg caaggaggca gaaaacgatg aaaccgaaaa
     1921 tccaaaggca cggagagatt ttaagccttg tccgccggca tcagtttatg tcggtggacg
     1981 agcttgccgc cgcattggac gttaccccgc agacgatacg ccgcgacatc cgcgaattgg
     2041 aggatgctgg cagcctgaaa cgccaccacg gcggcgcatc ctcgggcgga aacttgccgg
     2101 aggggctgcc cgccgaccgc caaacccggt gtcaaaacga aaaaaacgcc attgcccggc
     2161 tgattgcgga acacatcccc gacggttcgt cgctgtttgt cagtatcggc acgacgatgg
     2221 aggccgtggt gtcggagctg gtcaggcggc gcggcagcct gagggtgatt accaacaata
     2281 tccacgccgc ctcgattgct tcggcgcgga cggattatac ggtcatcatc acttccggag
     2341 tcgtccgccc tttggacggc ggtattaccg gcgtggcaac cgtcgatttt atcaaccagt
     2401 tcaaagtcga ttatgccgtg atgagtacgc acggcgtgga aagcgacggt tcgcttttgg
     2461 acgacgacta caaagaagtc agcgtgatgc aggcgatgat tgccaatgcg cgcgtccgct
     2521 tcctcggcgt ggaccacagt aaattccgca gcaacgcgct ggtcaggctc ggcgacatta
     2581 cggcgtttga caaagtattt accgaccggc tgcccgatac cgcgatgcag aagatgctga
     2641 aagaggcggg ggtggaatgc ttgattgccg atgccgtctg aacgctatgt caaagcgcgc
     2701 aagtcgggta caataaacac atcatcaaac cgcttcagac ggcataagga accgccccaa
     2761 tgccgtctga agccatctgt tcaaagaaaa ctatgctcaa taaagaccaa ttcgcggaca
     2821 accactttat ccgcaccatc atcgaagaag acctcgaaag cggcaaacat acagctgtcc
     2881 aaacccgttt tccgccggag cccaacggct acctgcacat cggacacgcc aaatccatct
     2941 gcctgaactt cggtttggct tatatttatg acggtttgtg caacctgcgt tttgacgata
     3001 ccaaccccga aaaagaaaac gacgaatacg tcaacgccat caaagaagac gttgagtggt
     3061 tgggcttcca ttgggcgggc gagccgcgtt tcgcttccaa ctatttcgac cagctttatg
     3121 actacgccgt cggtttggtt aaagacggca aagcgtatgt cgatgatttg acgccagaag
     3181 aaatgcgcga ataccgtggt acgctgaccg aagcgggcaa aaacagccct taccgcgacc
     3241 gcagtatcga agaaaacctc gacctgttca cgcgtatgaa aaacggcgag ttccccgacg
     3301 gcagcaaaac cctgcgcctg aaaatcgaca tggcttcggg caacatcaat atgcgcgatc
     3361 ccgtcatcta ccgcatccgc cgcgcctatc accacaacac cggcgacaaa tggtgcatct
     3421 atccgatgta cgactacaca cattgcattt ccgatgccat tgaaggcatc acgcattccc
     3481 tgtgtacgct cgaatttgaa gcacaccgtc cgttgtacga ctgggtgttg gacaacattc
     3541 ccgcgccgca cgccacccgt ccacgccaat acgaattttc ccgtttggag cttttgtaca
     3601 ccattacctc caaacggaaa ttgaatcagt tggttgtgga aaaacacgtt tccggctggg
     3661 acgatccgcg tatgccgacg atttccggta tgcgccgacg cggttacacg cccgaaggcc
     3721 tgcgcctgtt tgccaaacgc gccggtattt ccaaatctga aaacatcgtc gatatgagcg
     3781 tgttggaagg cgcgattcgc gaagagctgg aaaactccgc cccgcgcctg atggcggtgt
     3841 tgaacccgct caaagtaacc ctgaccaact ttgaagccgg caaaacccaa agccgccgtg
     3901 ccgcgttcca tccgaaccac gaagaaatgg gcgagcgcga agtacctatt tcacaaacca
     3961 tctacatcga agccgacgac tttgccgaaa atccgcccaa aggcttcaaa cgcctgattc
     4021 ccggcggtga agtacgcttg cgccacggct atgtcatcaa gtgcgatgaa gtcgtcaaag
     4081 acgaagcagg caatgtggtc gaactcaagt gcagcatcga ccacgacacc ttgggcaaaa
     4141 atccagaagg ccgtaaagtt aaaggcgtga ttcattgggt ttccgccgaa cacgccgccg
     4201 aaatcaaagt ccgcctgtac gaccgcctct ttaccgtcga gcgtcccgat gccgtgcgcg
     4261 gcgaagacgg cgaatacctg ccgtttaccg atttcctcaa tccggaatcc gttaaggaaa
     4321 tcactgctta cgccgaacct gccgccaaag atttgccggc agaaagccgt tggcagttcg
     4381 agcgtatcgg ctacttcgta accgaccgca aagaccacgg caaagacacg ccggtgttca
     4441 accgcacggt aacgctgaaa gattcttggc agcctaagta aaacctcatc tttgccgtct
     4501 gaatattgtt cggacggcat ttcccctttg cccgcgaaat gcggcacatt cggcacaccg
     4561 gaaaggaaat atgatgaaag tcctctttat cgccgacccg atggcaagtt tcaaaactta
     4621 caaagacacc acctacgcaa tgatgcggga aatggcaaaa cgcggctggc ggctgtttca
     4681 taccttgagc agcggattgt cggttaaaaa cggtctggtg gttgctgatg cgtctgcttt
     4741 tgagtttctc ggcgcacaag acgaccacga caaaaactgg tttgccgccg ccgacaaagt
     4801 tcagacggca ttgaaagcat ttgatgccgt gattatgcgt accgatccgc cgttcgatat
     4861 gcaatacctc tacgccaccc aattactgac gctggcggaa cagcagggcg cgaaagtctt
     4921 taacagcgga cagtcgatgc gtgacttcaa tgaaaaactg gcgattttga acttcagccg
     4981 ctttaccgcg cccacgctgg taacgacccg ttccgccgat gtccgcgcat ttttgaaaga
     5041 acacggcgac attatcatca aaccgctgga cggtatgggc ggtatgggta ttttccgcct
     5101 gaccgaaaaa gaccccaaca tcggcagcat cctcgaaacc ctgatgcggc ttgattcccg
     5161 caccattatg gcgcaacgct acattcccga aatcgtacac ggtgacaaac gcatcctgat
     5221 tatcggcggc gaagtcgtcc cctatgcttt ggcgcgtatc ccgcaaaacg gcgaaacacg
     5281 cggcaacctt gcggcaggcg gacgcggcgt ggcgcaggaa ttggacggac gcgaccgcga
     5341 aattgccgaa acgctcgccc ccgaactcaa acggcgcggc atcctgcttg ccggtttgga
     5401 cgttatcggc agcaacctga ccgaagtcaa cgtaaccagc ccgaccggat tccaagaaat
     5461 catgaaacaa aaaggtttcg acgtggcggc aatgtttgcc gatgccgttg ccgcgtggtc
     5521 ggtacgttaa accgatgccg tttgaaaggc ttttgcttca caaccgtttg gtttgttcgg
     5581 gcaggcaggg ttttccctca gccggcagag atacgcttaa tgccgtacgg acacaccgcc
     5641 gccccgtttt cagacggtat atatagtgga ttaacaaaaa tcaggacaag g
//
LOCUS       DAWFJR010000112         5453 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWFJR010000112 DAWFJR010000000
VERSION     DAWFJR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.112"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(117..272)
                     /gene="rpmG"
                     /locus_tag="WGR04_001873"
     CDS             complement(117..272)
                     /gene="rpmG"
                     /locus_tag="WGR04_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ0795372.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(304..537)
                     /gene="rpmB"
                     /locus_tag="WGR04_001874"
     CDS             complement(304..537)
                     /gene="rpmB"
                     /locus_tag="WGR04_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ0795373.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(787..2313)
                     /gene="farB"
                     /locus_tag="WGR04_001875"
     CDS             complement(787..2313)
                     /gene="farB"
                     /locus_tag="WGR04_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ0795374.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     ALVFFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKHRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGIIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHSSGGGGH
                     "
     gene            complement(2327..3490)
                     /gene="farA"
                     /locus_tag="WGR04_001876"
     CDS             complement(2327..3490)
                     /gene="farA"
                     /locus_tag="WGR04_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ0795375.1"
                     /translation="MDTHTDETKLQNTQAKRKRCLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGDVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGNPRPDAV"
     regulatory      3883..3927
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3931..4404
                     /gene="queF"
                     /locus_tag="WGR04_001877"
     CDS             3931..4404
                     /gene="queF"
                     /locus_tag="WGR04_001877"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ0795376.1"
                     /translation="MSRNTEELQGISLLGNQKTQYPAEYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            4481..5167
                     /locus_tag="WGR04_001878"
     CDS             4481..5167
                     /locus_tag="WGR04_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ0795377.1"
                     /translation="MYAFTDAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
BASE COUNT         1313 a         1551 c         1310 g         1279 t
ORIGIN      
        1 tccttggcag acggtttgca gcagattcgc atacggcaga tgtttcaagc ggaaaagaaa
       61 cacgataaaa ataaaaaagc ctccgaacag tcggaggctt tgctttcaaa ctgaaattat
      121 ttcagtttgg tttctttgta cactacgtgt ttgcgggcaa ccggatcgaa ttttttaatt
      181 tccagtttgc cgggcatagt gcgtttattt ttggtagtgg tgtagaagtg accagtacct
      241 gcactggatt ccagtttgat tttatcgcgc attgcagtaa tccttaatta aatagtgttt
      301 aaattaagct tcgccgcgag cacgcaaatc agccaatacg acatcaatgc ctactttgtc
      361 gatggtacgc agtgcagcgt tggaaacgcg caggcgaacc cagcggtttt cactttctac
      421 ccaaaaacga cgtgattgca agttgggcaa aaaacggcgt ttggttttgt tgttggcgtg
      481 cgatacgttg ttgccggaca tcgggcgttt gccggtcact ttgcaaactc gtgccataat
      541 cagtctccaa acttctaaaa tagtaaaacg cgatttatac cacgaaaaac agtgttagtt
      601 caagtgtttt aaagaaaaac ggctgttttg cgcggataat ccttccgatt gtcgcatctg
      661 cggaacgttt gcaggtgcga aacagatggg gcgcgattat agcggatttc gggaactgct
      721 gcattcaaaa tgccgtccga acatatttcc agtattttca gacggcattt caagttttca
      781 aatccctcaa tgtccaccgc cgccactgct gtggaacggc ggttttgcca gccatatgac
      841 gggtatcatg ataatgaata acaagctgcc tgccataaag atttcattcg atccgatgat
      901 gaagccctgc tgggtaatgg tattgttgat gatacctagg gtttgaatgt cggaaatgcc
      961 ttgttgggac aaatgcgccg ccgtttcgtg caatgttgcg gaatagggcg tgatgtgttc
     1021 ggcaaagcgc gtgtggtgca acgcttcgcg ccgttcccac agggtgctga cgacggatac
     1081 gccgacaccg cccatcagca cgcgcaggaa attcgacagg ctgccggcgg cggcaatctg
     1141 cccgcccttc atgtgcgaca gtgtgatggt ggtcagcggc aggaagaaca tggcaacgcc
     1201 tactccctgc caaaactgcg gccagatgac gttgccgata tccatgtcgg cataaaaatc
     1261 ggtgcgccaa tagaaagtga aggcaaaggt caggaagctg gcggttacga gcaggcgcat
     1321 atcgatttta ttgccgaacc tgccgattaa cggagacagg aagacgggca ggatgccgac
     1381 gggcgcggcg gcaagccccg cccacgtgga ggtatagccc aggttggtct gcaacactaa
     1441 cggcagcagg gtcagcgtcc ccatatacac cataaaaccc aatgatgtgg caatgatgcc
     1501 gacggtaaaa ttccgatctt taaacagcga taaatcgaca atcggatatt tttctcccaa
     1561 ttcccaaaca ataaaatacg acaagcacac cagtgcgact acgcccaagg taatgatttc
     1621 tccagaggcg aaccagtcga gttccttacc cctgtccagc atcatctgta acgcgccgat
     1681 accgactacc atcaatgtaa gcccgacata gtcggtcggc attttcacgg tttccgtttc
     1741 ccgatgtttc aaatgtttcc atgttatcca tgccgatatg ataccgatag ggatattaat
     1801 gaagaaaatc caaccccaat gccagtttcc ggaaatccag ccgccgagta tcggcccgag
     1861 aacaggggcg acaacgacgg tcattgccca caatgccagt gccagcgtcc gttttgcggg
     1921 cggataggat gccattaaca ggctttgcga caggggaatc agcggcccgg cgataaagcc
     1981 ctgcaagatg cggaaaaaca ccaaagcctg aaggttgggc gcgatgccgc acagccatga
     2041 tgtgatgacg aaaccgacag cggcggcggt aaacaatttg acctcgccga tacgttttgc
     2101 caaaaagccc gttaacggca cggaaacggc gtttgccaca gcaaaagaag taatgaccca
     2161 cgtcccctgc gtggttgccg caccgaggtt gccggcgatg acgggaacgg cgacattggc
     2221 gatagtcgta tctaaaactt ccataaatac ggcaagcccc aaagacagcg taacccacgc
     2281 caatgccgca cccttaagcg gtggataatc cattgtgtcc tccgtttcag acggcatcag
     2341 ggcgcggatt gccgaggatt tcatcgaccg tccgatcgac ttccgaccag tcggtacttt
     2401 ccatttccgg caatgccgca cccggcgttt ttgaaacagg cgcgcctgcg gcagaagtat
     2461 ccactttaac cgtcatcgac aaaccgatac gcaacggatg cctgtccaca tcttcgcggt
     2521 tcagcacgat acggacaggg acacgctgca ccactttaat ccagttgccc gtggcatttt
     2581 gcgccggaat cagcgaaaac gcgctgcccg tacctgccga aaaccctgcc accctgccgc
     2641 gataaacaat ttgtttgccg tacaaatcgg acaccagttc ggcaggctgt ccgattttca
     2701 tatgccgcaa ctgcgtctct ttaaaattgg catccaccca cacatccgac agcggcacca
     2761 ccgccatcag cggcgcgcct gccgccacct gctgcccgac ctgcaccgaa cgcttcgcca
     2821 cctgaccgtc cgccggcgcg cggatttgcg tccgctgaag gttcaaccac gcatctttca
     2881 acctgccgat tgccgtctga accgccggct gttcgcgcaa agaaacatct ccgcccaaag
     2941 ccgcacgtgc cgaagattct tccgccaaag ccgccttaac tgccgcctgc gcctgagaca
     3001 ctgccgcacg ggcgtgtgcc agctcttcgg cggacaccgc gcccgattcc gccaaagcag
     3061 accggcggcg caaatcatcc tgcgcgcgtg ccaaatccgc ccggcgcaag gcaacctgcg
     3121 cccccgcctg cgaagtggcg gcattttgcc ggcggttttg ccgcaccgcc tgaaccagct
     3181 cgttttttgc ccgctcgtaa gccagcacat cattatcgtc gtccaatacc gccagcacgt
     3241 cgcctttttt cacggcatcc gtatcgtcgt gcaaaacctt ccgcaccgta ccgccctttt
     3301 gcggcgtaac ctgaaccacg cgtccggcaa cataagcgtc ttccgtttcc tcttcgtgct
     3361 gccaccataa aaaaaacgcc gacccggcgg ctgcggcggc aagcgcgaac agcagcgtca
     3421 atgccgtcag gcagcgtttg cgtttggctt gcgtgttttg aagttttgtt tcgtccgtgt
     3481 gcgtatccat aagattgggt tccgaatttc ctgatttcat ataaacaatg ttctttcaat
     3541 aggaggcaga ttctatatcc gcgcaataac gcaatcaagt cagtatttta gatgcaatat
     3601 tcaaaaaaca acgcttcagg cggcaaaact gaaaatatat ttcgtttata tagtggatta
     3661 acaaaaatca ggacaaggcg gcgagccgca gacagtacgg atagtacgga accgattcac
     3721 ttggtgcttc agcaccttag agaatcgttc tcgttgagct aaggcgaggc aacgccgtac
     3781 tggtttttgt tcatccacta tatatgacac ggaaaacgcc gccgtccaaa ccatgccgtc
     3841 tgaagaaaac tacacagata ccgccgctta tattacaatc gccgccccgt ggttcgaaaa
     3901 cctcccacac taaaaaacta aggaaaccct atgtcccgca ataccgaaga gctgcaaggc
     3961 atctcgcttt tgggtaatca aaaaacccaa tatccggccg aatacgcgcc cgaaattttg
     4021 gaagcgttcg acaacaaaca tcccgacaac gactatttcg tcaaattcgt ctgcccagag
     4081 ttcaccagcc tctgccccat gaccgggcag cccgacttcg ccaccatcgt catccgctac
     4141 attccgcaca tcaaaatggt ggaaagcaaa tccctgaaac tctacctctt cagcttccgc
     4201 aaccacggcg attttcatga agactgcgtc aacatcatca tgaaagacct cattgccctg
     4261 atggatccga aatacatcga agtattcggc gagttcacac cgcgcggcgg catcgccatt
     4321 catcctttcg ccaattacgg caaagcaggc accgagtttg aagcattggc gcgtaaacgc
     4381 ctgtttgagc acgacgcaca ataaaccatc cttcccatat ttcagacggc attccttcga
     4441 tacgatatgc cgtctgaacg cctttccgaa aggttttttt atgtacgcat ttaccgacgc
     4501 acagcaacag aaggcactct tctggctggt gctttttcat atcctcatca tcgccgccag
     4561 caactatctg gtgcagttcc ccttccaaat tttcggcatc cacaccactt ggggcgcatt
     4621 ttcctttccc ttcatcttcc ttgccaccga cctgaccgtc cgcattttcg gttcgcactt
     4681 ggcacggcgg attatctttt gggtgatgtt ccccgccctt ttgctttcct acgtcttttc
     4741 cgttttgttc cacaacggca gttggacagg cttgggcgcg ctgtccgaat tcaacacctt
     4801 tgtcggacgc atcgccttag ccagctttgc cgcctatgcg ctcggacaaa tccttgatat
     4861 ttttgtattc aacaaattac gccgtctgaa agcgtggtgg attgccccaa ccgcatcaac
     4921 cgtcatcggc aacgccttgg atacgttggt atttttcgcc gttgccttct atgcaagcag
     4981 cgatgaattt atggcggcaa actggcaggg catcgctttt gtcgattacc tgttcaaact
     5041 caccgtctgc ggtctgtttt tcctgcccgc ctacggcgtg attctgaatc tgctgacgaa
     5101 aaaactgacg accctgcaaa ccaaacaggc gcaagaccgc cccgcgccct cgctgcaaaa
     5161 tccgtaaaac agccctgccg ggaatcggga cggttgggtt ttcttttatt tgcaaacact
     5221 gtcggaacgg ataaatccgg attcccgccc acttcgtcat tcccgcgaaa gcgggaatcc
     5281 ggtacgttgg gtttcagtca tttccgataa actcctgccg cgttgtattt ctagattccc
     5341 gctttcgcgg gaatgacggc ggatgggttt ccgtgccgtg gtgtccggat tcccgcccgt
     5401 acgggaatga cggcagtgct tccttggctt agttttattt taatcgactg tat
//
LOCUS       DAWFJR010000113         5432 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWFJR010000113 DAWFJR010000000
VERSION     DAWFJR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.113"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(234..3065)
                     /gene="lbpA"
                     /locus_tag="WGR04_001879"
                     /gene_synonym="tbpA"
     CDS             complement(234..3065)
                     /gene="lbpA"
                     /locus_tag="WGR04_001879"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ0795378.1"
                     /translation="MNKKHGFQLTLTALAVATAFPAYAAQAGGATPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTYDIKRKTREPFFSVEGERESK
                     PVAKLAGYGKYLNNQLNRWVKERIEQNQPLSAEEEAQVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDTNLVKGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEKYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISESTG
                     FDDKNQDKYLLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSLSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADH
                     KTQLPDSTGRLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(3078..5276)
                     /locus_tag="WGR04_001880"
     CDS             complement(3078..5276)
                     /locus_tag="WGR04_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0795379.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTPPPAGSSVETTPVNRPAVGAAMRLPRRNIASYKQDGTEIPDKHQAEEHLPLKEK
                     DILFLDGTLKEQADKLKKKINERYSDVRVITSKKEEEKYQYQFVRAGYVFTRAEGKDN
                     EKEKTSDGKEFVNRFSYDGFVYYSGERPSQSLPSAGTVQYSGNWQYMTDAKRHRTGKA
                     VSSTDLGYTTYYGNEIGATSYEARDADDREKHPAEYTVDFDNKTLNGKLIKNQYVQNK
                     SNPNEPKKPLTIYDITATLDGNRFTGSAKVSTEVKTQHADKEYLFFHTDADQRLEGGF
                     FGDNGEELAGRFISNDNSVFGVFAGKQKTETENAADTKPALSSGKHTKILDSLKISVD
                     EASDKNPREFAISSMPDFGHPDKLLVEGREIPLVNKEQTIELADGRKMIVSACCDFLT
                     YVKLGRIKTDRPASKPKAEDKGEDEEGAGVGTVEEDEIGDEESTGDEVVEDEDEEEPE
                     EEAEEEAEEEAEEEETKEKSPTEEGNGGSGSILPALEAPKGRDIDLFLKGIRTAEADI
                     PKTGTAHYTGTWEARIGEPIQWDNQADEKAAKAEFTVNFGEKSISGTLTEKNGVQPAF
                     HIENGKIEGNGFYATAHTRDNGINLSGTGSTNPQSFKASNLRVEGGFYGPKAEELGGI
                     IFNNDGKSLGITEGTENKVEAEVEADVGKQLEPDEVKHKFGVVFGAKKDN"
BASE COUNT         1158 a         1460 c         1250 g         1564 t
ORIGIN      
        1 cgtcattccc acgaacctgc atcccgtcat tcccacgaaa gcgggaatcc agttttttga
       61 gtttcagtca tttccgataa attgccttag cattgaatgt ctagattccc gcctgcgcgg
      121 gaatgacggc ggaaatcttg tttatattga atcaaaaaaa acctgcacct taatcagttg
      181 gcggtttagt ccgacttttg gggtgcagat caagctttca gacggtattt cctttaaaac
      241 ttcatttcga gcgcgagact gaagttcctg cccggtgcgg cataccttcc atagttgctg
      301 tcgccgccgt gccggtttgc cgtgctttcc gcagtctggc gcaaggattc ccaagtaacg
      361 tagcggtagt tgccgatatt gtagatagcc gccctcaagg tcagccgttt tttcagattc
      421 agataggcgg aaacgtctgc cgtcgaccaa gaagacgacg ctctttttgt cgaatatcgt
      481 ttttgatcgc ctgccagata agcaagctcg tcagggtttt tccctttgga ataggtcagc
      541 ataatgtttg cgccccattt cccctcgggc tggtcgtatc cgaaccccaa aacataacgc
      601 gacggctgta ccgcatccaa agcatagctg cggagggaca gtcccggccg gttggatacc
      661 gatttcggtt tgatacggtt gtacgccaat gtggtgtaca aaccttcggg cagtttgccg
      721 tacacgccgt tccagtcgat ttttcccaat atattaacgc cttggagcga catattttgg
      781 gcattgtaat aatcgcgtat atcaatctct gtcaatcgtc ctgttgaatc cggcaattgg
      841 gttttgtgat cggcaacggc aatcatatcg gtatagcggt tgcggaagct gctgatttcc
      901 aaaaagccga aatcgccctt ccactgcaaa ccgatttcgc ggttggctgc cttttccgat
      961 ttcagggcgg gacgctgcca gcctttcgga taatcgtgat aaatgtctat cccgaaaagc
     1021 tcttggaatg agggcgttct gaagccgctg gaggcgcggt aagacaaaga aaaatgccgg
     1081 ttcggtttga acacgatgcc gctgttccac gaacggtcaa cataccgccc gctgcggacg
     1141 agttcttccg acgtggtgaa gtttttccgg tcgtacctgc cgcccaagct gaaatcaaaa
     1201 tatttgccga ttgaaaaacg gtcgttcaaa gaaatgtgga tattgctgcc gttgattttt
     1261 cttggcacgc atttgcggga acgcaaagtt tcgatgtagc cgcagaccga cccttcgacg
     1321 acttcgggct tacccaaaag atacttatct tgatttttat catcgaatcc cgtggattcc
     1381 gaaatccttg ccgcattgtg ggaaagctgt tcggggcggg agatcgcttt ggaagcatcg
     1441 taaccgaagc ccaaagtcag atggtgtttc gtccatttgt ttttcagcga tttctcaaac
     1501 gaggcattca aaacattgtg ctgttcgcgg tagtggaaac ggtcgctgct gtcgtaggaa
     1561 tacggtttgt ccgccgacgc gcggcaggat ttgtccacgg caggatacac ggcgcaattc
     1621 agcttcagcg tgttgttgtc ggttgccacg ccctgtttgt caaacgacaa caccgcctta
     1681 tccgcccagt tgtccgagta tttctcattt tcataacgat acagcaaacc catacggcgg
     1741 cggcggtgat gttcgtcaat aaatttggtg cgggaatatt tcaaacctat gcccttgacc
     1801 aaatttgtat cgcccttcca ctcttctata ttcggcacaa aatacaagcc gtcgcggaaa
     1861 tcgtcgccgt cgtacacccc gctcttgtct ctaaactttt ccgcctcgtc cgtaccgtaa
     1921 tactgttttt ccgtcatatc gcggatatcg taacgctgtt tggtatcctc aaacacgccg
     1981 ccgacataat gcctgccgcc gaagcggtag cccagcttgg caagccaaga gccgctgcgg
     2041 taatccatcg gatcgggcaa tatcctgccg ccgcccgtgt aagcttgggc ggacagattt
     2101 tcgtggcgcg cctgcgcctc ccgcacctgc gcctcttctt cagcacttaa aggctgattt
     2161 tgttcaatac gttcttttac ccagcggttg agctggttgt tcaaatattt cccgtagccc
     2221 gccaattttg ccacgggctt ggattcacgc tcgccctcta ctgagaaaaa tggctctctt
     2281 gtcttgcgtt taatatcgta tgtctgacgg aacgcgtcca aacggtttat gccgtatgcc
     2341 accccgtccg caatatcgcc gtgcgggcgc gtttcccgcc cttggcgttc ggttcggatt
     2401 agcagccctt cccaaccgtc tttgctgaaa cccgcgccga gcgacttcat aaattggcgg
     2461 tttttactgc cgtaggcggt ttttgcctgt atcccccagc ttttgccgtc tgaaatcagg
     2521 tctgccgctt ctttggtgcg gaaagcgacc gcgccgccga gtgcgccgct gccgtgatcg
     2581 gacgaaccgg cacctttgtc gatttccacc gtgctgatgt tttcatattc gatttcgttg
     2641 attgcaccgc tgccgccgcg tccgccgtat ccgctcaacg atccctgcac ggtaaacgcc
     2701 tgtatttggg caacaccgtc gaccgaaacc gccacacggt ttttatccac gccgcgtatc
     2761 gagtagccgc cgctcgcgcc gttgccctgt tcgacaaccg ccacgcccgg atcgtagcgc
     2821 gtcaggtcgc ggataccgag tacctgttct ttgttcaacg tttccgacgt tttgacgatt
     2881 ttgcccaaac cggtcgcctc tttcgatcgc cgtcccactt tggcggcacg gacggtaacc
     2941 tctttcaggg attgggtctg cgccgcatca ggtgtcgctc cccccgcttg ggcagcataa
     3001 gccggaaaag cggttgcaac agccaaggca gtcagagtaa gctgaaaacc gtgtttctta
     3061 ttcatttttc cacctcttta attatctttc ttcgcaccga ataccacgcc gaatttgtgt
     3121 ttaacttcat caggttctaa ctgtttgcca acatcagctt caacttcagc ttcaacttta
     3181 ttttcagtac cttcagttat accaagagat ttcccatcat tattgaaaat aataccgccc
     3241 aattcctccg ccttcgggcc gtaaaatcct ccttctacac gaagattact ggctttgaaa
     3301 ctttgaggat tagtcgaacc agttcccgaa agattgatgc cgttatcccg agtgtgtgct
     3361 gtcgcgtaga aaccgttgcc ctcaatcttg ccgttttcaa tatggaaagc aggttgtaca
     3421 ccgtttttct ccgtcagcgt tccagaaatc gatttctcgc cgaaattaac ggtaaattct
     3481 gcttttgccg ctttttcatc cgcctgattg tcccattgaa tgggttcgcc gatacgcgct
     3541 tcccaagtgc ccgtataatg cgccgttccg gtttttggaa tgtcggcttc cgccgtgcgg
     3601 atacctttca ggaaaaggtc gatgtccctg cctttagggg cttctagggc aggcaggatg
     3661 ctgcctgaac cgccgttgcc ttcttctgtc ggcgattttt ctttggtttc ttcctcttca
     3721 gcttcttctt cagcttcttc ttcagcttct tcttcaggtt cttcttcgtc ttcatcttct
     3781 acgacttcgt ctccagtgct ttcttcatcg ccgatttcgt cttcttcgac ggtaccaacg
     3841 cctgcaccct cttcatcctc ccctttatct tccgcctttg gtttacttgc cgggcggtcg
     3901 gtttttatcc gtccgagttt cacataggtc agaaaatcgc aacaagcact gacgatcatt
     3961 ttcctgccgt cggcaagctc gatggtttgt tctttgttta ccaaaggaat ttcacgccct
     4021 tcgacaagaa gtttgtcggg atgaccaaaa tcgggcatag aggaaatggc aaactcacgg
     4081 ggatttttat cacttgcctc gtcaacggaa attttcagag aatccaagat tttggtgtgt
     4141 tttccagacg acagggcagg ttttgtatct gctgcgtttt ctgtctctgt tttttgtttg
     4201 cctgcgaata cgccgaatac gctgttgtcg ttgctgataa accgtccggc aagctcttct
     4261 ccgttatcgc cgaaaaaacc gccctcaagc cgctgatcgg catcggtatg gaaaaacaaa
     4321 tattctttat cagcgtgttg cgtcttcacc tcggtgctaa ctttggcact gccggtaaag
     4381 cggttgccgt ccaatgttgc ggtaatgtcg taaatggtca gcggtttttt gggctcattt
     4441 ggattacttt tattttgcac atactgattt ttaatcagct tgccattcag ggttttgtta
     4501 tcaaaatcaa ccgtatattc ggcaggatgc ttttccctgt cgtcggcatc cctagcctca
     4561 taagaagttg ccccaatttc attaccataa tatgtggtat aacccaaatc cgtactggaa
     4621 accgccttac ctgtccgatg acgtttggca tcggtcatat attgccagtt accggaatat
     4681 tgcaccgttc ccgcgctcgg taaagattgg gaaggacgtt ctccggaata atatacaaaa
     4741 ccgtcataac taaatcggtt aacaaactcc ttaccatcag aagtcttttc tttttcatta
     4801 tcctttcctt ccgccctggt aaacacatag cccgcacgga caaattgata ttgatatttt
     4861 tcttcttctt ttttcgatgt gataaccctc acatcagaat accgttcgtt gattttcttt
     4921 ttaagtttgt cagcctgttc tttcagcgta ccgtctaaaa acaggatatc cttctcttta
     4981 agcggcagat gctcctctgc ctgatgcttg tcgggaattt ccgtaccgtc ttgtttatag
     5041 gaagcaatat tccgccttgg cagccgcatt gccgcaccga cggcgggccg gttgaccggc
     5101 gtggtttcta ccgaagaccc ggcagggggc ggagtgggaa cgtccttaga tttgaaggtg
     5161 acggggtacg cggtcggcgt tgattcgaca acaggctgca cgccgaaatt gccgccgata
     5221 caagatgcta aaagtaaggg caacaagaca atgccgccat aattcggttt acacatccct
     5281 acttttcctc tatttgatta ataataatta tcattatatt aatatgtaca gataatatca
     5341 agccgttttt atagtgaatt aacaaaaatc cggacaaggc gacgagccgc agacagtaca
     5401 gatacattcc gtcattccca cgaacctgca cc
//
LOCUS       DAWFJR010000114         5247 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWFJR010000114 DAWFJR010000000
VERSION     DAWFJR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.114"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..479)
                     /locus_tag="WGR04_001881"
     CDS             complement(<1..479)
                     /locus_tag="WGR04_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0795380.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAVLFY
                     RKIRMVISHYLALAANEVFEGSVELDESYFGGRRKGKRGRGAAGKVVVFGILKRNGRV
                     YTVVVDNAKSDTLMPVIKQKIMPDSIVYTDSLSSCDKLDVSGFIYYRINHSKEFADR"
     gene            816..1250
                     /locus_tag="WGR04_001882"
     CDS             816..1250
                     /locus_tag="WGR04_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795381.1"
                     /translation="MDNKKEFINNLTNRYMWIYPLVLNILFLPFYQSYQSFFIALGCL
                     FALVRKMQSLDFKLQNHIVLLNIKSAWADKKVFLIRIVVSWLAVMEIWMCFISESSTW
                     VCGAFCLNSEILEKIFRGFGYSGSLYFLFILMIDLNKLRESI"
     gene            1526..2245
                     /locus_tag="WGR04_001883"
     CDS             1526..2245
                     /locus_tag="WGR04_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795382.1"
                     /translation="MKNKNYLLVFIVLHIALIVINIVFGYFVFLFDFFAFLFFANVFL
                     AVNLLFLEKNIKNKLLFLLPISIIIWMVIHISMINIKFYKFEHQIKEQNISSITGVIK
                     PHDSYNYVYDSNGYAKLKDNHRYGRVIRETPYIDVVASDVKNKSIRLSLVCGIHSYAP
                     CANFIKFAKKPVKIYFYNQPQGDFIDNVIFEINDGNKSLYLLDKYKTFFLIENSVCIV
                     LIILYLKFNLLLYRTYFNELE"
     gene            2463..3401
                     /locus_tag="WGR04_001884"
     CDS             2463..3401
                     /locus_tag="WGR04_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ0795383.1"
                     /translation="MIASRLSGNISSAIQEHKDGKVNINRFQNILADLYSLGGLGSTL
                     IEKNGNMQSWGIPLAIAGDIIAATAIATGDTGTISTEEFYNFDNWKGFGYELFEDWSR
                     WVYDWLPDGWNLWKELDRNRSGQYHIYDPLALDLDGDGIETVAAKGFSGSLFDHTNNG
                     IRTATGWVSADDGLLVRDLNGNGIIDNGAELFGDNTKLADGSFAKHGYAALAELDSNG
                     DNIINAADAAFQTLRVWQDLNQDGISQANELRTLEELGIQSLDLAYKDVNKNLGKESK
                     PQSAVLAIHTPFVCFLGNLKGIICTRHNPILYFTKN"
     gene            4691..5247
                     /locus_tag="WGR04_001885"
                     /pseudo
     CDS             4691..5247
                     /locus_tag="WGR04_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1667 a          894 c         1000 g         1686 t
ORIGIN      
        1 tgacggtctg caaattcctt ggaatggttg atgcggtaat aaatgaaacc gctcacgtcc
       61 aacttgtcgc agctgctcag actatcggta taaacaatgc tgtccggcat gattttctgt
      121 ttgataacag gcattaaagt atcggacttg gcattatcta cgacaacggt atagacccgt
      181 ccgttacgtt tcagaatgcc gaagacaacc acttttcctg ccgcaccgcg accacgtttg
      241 cctttacgcc gtccgccgaa atagctttcg tccaactcga cagagccctc gaaaacctca
      301 ttggcagcca aggccagata atggctgatg accatacgga ttttgcggta gaacaggact
      361 gccgaattgg gatggatacc caaaatatcg gcagcagaac gggcggtaac ttcgagtaca
      421 aaaaaacgga gcagctcttt ctgtactttt ttctttaatt tgcagtgtgt tatcttcata
      481 tttcgggggt aacatatctg ctaatctagt acagcctcaa acaaaaaaga gaaattttaa
      541 tttcgctaaa tcgcataaag attaatcaag agtatcatta aatgatatga gtgagcatct
      601 ataatgccaa gaagagttgt taagacataa cgattattga aatagattgt aaaatagata
      661 cttagatagt ctgaaaaacg gatttgtgaa actttttatt acgcgccatc atttgaaaat
      721 gaaacttaaa aaacacttat cataataaat attttcttta cgttgtttgc taataaactc
      781 agtgcaatat cagcgcaata ttttatggaa attttatgga taacaaaaaa gaatttatta
      841 ataatttaac aaataggtat atgtggatct atccattggt cttaaatatt ctatttctac
      901 ctttttacca gtcctaccaa tcttttttta ttgcgcttgg ttgtttgttt gcactggtta
      961 gaaaaatgca aagcttagat tttaaattac aaaatcatat tgtattgtta aatataaaaa
     1021 gtgcttgggc agataaaaaa gtatttttga ttaggatagt agtgtcatgg ttggcagtaa
     1081 tggaaatatg gatgtgtttt atttcggaat catcaacgtg ggtatgcggt gctttttgtt
     1141 taaatagtga aatattggaa aaaatttttc gtggctttgg ttattctggt agtttatatt
     1201 ttttatttat attgatgatt gatctcaata agttaagaga gagtatttga atttcatttt
     1261 ttgtttgact taaactcaag gagagtaaca atgattggta gtggtgatac taaacaatgc
     1321 aaaaaatttt ctgcgtgtga tggaaaatac cacgtctacg atcccctcgc cctagacttg
     1381 gacggcgacg gcatagaaac agtcaccgcc aaaggctttt caggcagcct gaagactgag
     1441 agagtgaata cgatgagtat acactctatg ccactaaatt gatattcact aaatcatacc
     1501 agctatattt tatttaatga gacatatgaa aaataaaaat tatttactag tatttatagt
     1561 tttacatata gccttgatag taattaatat agtgtttggt tattttgttt ttctatttga
     1621 tttttttgcg tttttgtttt ttgcaaacgt ctttcttgct gtaaatttat tatttttaga
     1681 aaaaaacata aaaaacaaat tattgttttt gttgccgatt tctattatta tatggatggt
     1741 aattcatatt agtatgataa atataaaatt ttataaattt gagcatcaaa taaaggaaca
     1801 aaatatatcc tcgattactg gggtgataaa accacatgat agttataatt atgtttatga
     1861 ctcaaatgga tatgctaaat taaaagataa tcatagatat ggtagggtaa ttagagaaac
     1921 accttatatt gatgtagttg catctgatgt taaaaataaa tccataagat taagcttggt
     1981 ttgtggtatt cattcatatg ctccatgtgc caattttata aaatttgcaa aaaaacctgt
     2041 taaaatttat ttttataatc aacctcaagg agattttata gataatgtaa tatttgaaat
     2101 taatgatgga aacaaaagtt tgtacttgtt agataagtat aaaacatttt ttcttattga
     2161 aaacagtgtt tgtatcgtat taattatttt atatttaaaa tttaatttgc ttttatatag
     2221 gacttacttc aatgagttgg aatagttttg gtaattttat gagcgcacgc tcatccgcgt
     2281 tagcagaatt tggaaatatg gttgctaatt tagtttctgc aaaaaatgag aaagatatct
     2341 cgaaacgtaa tgaatattac aaacaagctg gttatagtgc attattagca tttggtaatt
     2401 tggctagtaa tattgcacca ggtagtacgt catcgcatat tgtaaacgga acaaatgcct
     2461 ctgtgattgc aagccgtctc tctggaaata tatcttcagc tattcaggag cataaagatg
     2521 gtaaagttaa tatcaaccgt tttcaaaata ttttagcgga tttatattca ttgggagggt
     2581 taggaagtac attaatagag aagaatggaa atatgcagag ttgggggatt ccattagcaa
     2641 ttgctggaga tataattgca gcaacggcta ttgccacagg agatactggt acgatatcta
     2701 cagaggaatt ttataatttt gacaactgga aaggttttgg gtatgagcta tttgaagact
     2761 ggtctcgttg ggtatacgac tggctgcccg acggctggaa tctgtggaaa gaattggaca
     2821 gaaaccgttc aggccaatac cacatctacg accccctcgc cctagatttg gacggagacg
     2881 gcatagaaac tgtcgctgcc aaaggctttt caggcagctt atttgatcac accaacaacg
     2941 gtatccgcac cgccaccggt tgggtttctg ccgatgacgg tttactcgtc cgcgatttga
     3001 acggcaacgg catcatcgac aacggtgcgg aactcttcgg cgacaacacc aaactggcag
     3061 acggctcttt tgccaaacac ggctacgcag ctttggccga attggattca aacggcgaca
     3121 acatcatcaa cgcggcagac gccgcattcc aaaccctgcg tgtatggcag gatctcaacc
     3181 aggacggcat ttcccaagct aatgaattgc gtacccttga agaattgggt atccaatctt
     3241 tggatctcgc ctataaagat gtaaataaaa atctcggtaa agaaagtaag ccgcaaagtg
     3301 cagtacttgc tatacacaca ccgtttgttt gttttttagg caatctaaaa ggcatcattt
     3361 gcacccgaca taatccaata ctttatttta caaaaaatta acgtatttta acaaattaga
     3421 ttggattaat tgccctatta tctatattgg gttatcgggt tgctaattga gcagagcgta
     3481 gcaaaccata aagtgagtgt ttccacccac tctgcattta ctatgaagtg gttattaaat
     3541 atgataatga gacctattaa atttagtatg gtaaatacgt tattatttat tgttatatgt
     3601 agttcatttt ttgatctgct cgttcaatta tgtacaattt tatttcatag ccaaaaaata
     3661 tactttatta cattattttt attatttatt tttaattttg ctacaaaatc tatctatatg
     3721 gcaattattt atcctatttt atattttttt atcgtaaaaa aatattatcc ttactctagg
     3781 aaagtgataa ttctattatc attatcatta tctatatatt ttacttttat ggacttttac
     3841 tttttttcca tatattcaga taaccttagc tatgaaacag aacctttaca tttatacatc
     3901 cctattatta ttaacttttt ctcactttta gtttctaatt ttattttatc ttttatcaac
     3961 aagtaaaggc tcagttatgg aaatttttga ggtaaatgta tatacaaaat acaatccaaa
     4021 agatcctaag caaaatatta ttggtacagt taaagcttcg tatgtttcag atggagctgg
     4081 tggttatcta aaagataaga atggtaatcc ttatattgct cccgtcggat acaaccctca
     4141 tgaagcatgg gcttatggag cggagaatcc acttaatcta ctaaactatg gtacaggtaa
     4201 agaaaaagat ttgcagcgtt cattccatgg aaaatcgtat gggggatttg tgccagcgtt
     4261 tcagcccatt gcatcttttg atgttggagt agcttgtgct ggtgcaattg gtgtttctaa
     4321 ggatatttgc attgctggag gtggagcggt aaatttaagg aattcgttat ctaatagtaa
     4381 tatcaatact gaggggaaat actataacaa cccgcctaat gttccaaata tagaaaatgg
     4441 atatgattgg ggaaaaagcc ttggtacatt taatgattct ttagaaaatt cattaaaaga
     4501 aacattaaat aatatcagga ataaatttaa ggagattcag aattcatttc ataatttaac
     4561 agatagaaat atttcttttg attctgaaag cattggcagt ctcctgaagg agaaatttga
     4621 agactggtct catagggtat atgactggct acctgatgat tggaatctac ggaaagaatt
     4681 agacagaaat cgttcaggca aataccacgt ttacgacccc ctcgccctag atctagacgg
     4741 cgacggcata gaaacagtcg ccgccaaagg cttttcaggc agcctcttcg accataacgg
     4801 caacggcatc cgcaccgcca ctggctgggt tgctgccgat gatggtttac tcgtccgcga
     4861 tttgaacggc aacggcatca tcgacaacgg cgcggaactc ttcggcgaca acaccaaact
     4921 ggcagacggt tcttttgcca aacacggcta cgcagctttg gccgaattgg attcaaacgg
     4981 cgacaacatc atcaacgcgg cagacgccgc attccaatcc ctgcgtgtat ggcaggatct
     5041 caaccaggac ggcatttccc aagctaatga attgcgtacc cttgaagaat tgggtatcca
     5101 atctttggat ctcgcctata aagatgtaaa taaaaatctc ggtaacggta acactttggc
     5161 tcagcaaggc agctatacca aaacaaacgg tacaaccgca aaaatggggg atttactttt
     5221 agcagccgac aatctgcaca gccgctt
//
LOCUS       DAWFJR010000115         5209 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWFJR010000115 DAWFJR010000000
VERSION     DAWFJR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.115"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(90..1445)
                     /locus_tag="WGR04_001886"
     CDS             complement(90..1445)
                     /locus_tag="WGR04_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ0795384.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAASKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(1461..1733)
                     /locus_tag="WGR04_001887"
     CDS             complement(1461..1733)
                     /locus_tag="WGR04_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ0795385.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(1859..2596)
                     /locus_tag="WGR04_001888"
     CDS             complement(1859..2596)
                     /locus_tag="WGR04_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ0795386.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHQRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(2877..3776)
                     /gene="prmB"
                     /locus_tag="WGR04_001889"
     CDS             complement(2877..3776)
                     /gene="prmB"
                     /locus_tag="WGR04_001889"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0795387.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGNGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAHIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFDGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPKLALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            3921..4514
                     /locus_tag="WGR04_001890"
     CDS             3921..4514
                     /locus_tag="WGR04_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795388.1"
                     /translation="MADGIGNLIGWEKTGLVVGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHAASKANQAELSIITKLDDLLRYAKYEGSYSDKKGHPDIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDGHKHYDHFILKDE"
     gene            4626..>5209
                     /locus_tag="WGR04_001891"
     CDS             4626..>5209
                     /locus_tag="WGR04_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEZ0795389.1"
                     /translation="MTKLYAEIAKREAQDDGTVKVLGYASSEAVDSDGEVIATAAMKA
                     AIPDYMKFDAGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVSKVKTGVYKGFS
                     IGGSVTARDNLNKSQITGLKLTEISLVERPANPDAVSTCFKADKSAEAVNNDTEHNAT
                     YFSHFPSKQKSTGGSRCPFLYRFPYFFTAGSTGLA"
BASE COUNT         1421 a         1348 c         1157 g         1283 t
ORIGIN      
        1 attcttttct aaagacattt tacccgatgc ctataaacaa aaatgccgtc tgaagccttt
       61 cagacggcat ttccccatca aaaccgcaat cagtttttca tcgattgaac cggagccggg
      121 attctgccgc ctcggttaac aaacacttcg cacgaacctt ctttaaccgg catcacaggc
      181 gcgtagccca acaggccgcc gaactcgacg ctgtcgccga cggtttttcc ggttaccgga
      241 ataatgcgca cggcagtggt tttgctgttg atcatgccga tggcggcttc gtcggcaatg
      301 atgccggaaa tggtgtgcgc gggcgtgtcg ccgggaacgg caatcatatc caagccgacc
      361 gaacaaacgg cggtcatcgc ttcgagtttg tccagcgtca gcacgcctgc ttcggcggcg
      421 gcaatcatac cttcgtcttc ggaaacgggg ataaacgcgc cactcaaacc gccaaccgcg
      481 ctcgaagcca tcatgccgcc ctttttcaca gcatcgttca gcaatgccaa agcagccgtc
      541 gtgccgtgcg taccgcagac gctcaagccc atttcttcaa gaatgcgcgc caccgagtcg
      601 ccaacggcag gggtcggcgc cagcgacaag tcgagaatac caaacgggat attcagcatt
      661 tttgaggctt cgcggccgat gagttcgccc acgcgggtaa ttttgaaagc ggttttcttc
      721 actacttccg caacttcggt caatgtcgtt gcatctgaat tttccaacgc ggctttgacc
      781 acgcccggcc ccgatacgcc gacattaatc acagcatccg cttcgcctga gccgtgaaac
      841 gcgcccgcca taaacggatt gtcttccact gcattgcaaa acacgacgat tttcgcgcaa
      901 ccgaaacctt cgggcgtgat ttcagccgtg cgtttgatgg tttcgcctgc cagcttgacc
      961 gcatccatat tgataccggc acgcgtgctg ccgatattga tggagctgca cacgatatca
     1021 gtagttttca tcgcttcggg aatggaacgg attaacacct catctgaagg cgacatgcct
     1081 ttttgcacca gcgcggaaaa gccgccgata aaggacacgc cgatggcttt ggctgccttg
     1141 tccaaagtct gcgccacgct gacgtaagaa tcggctttgc tcgccgccgc gatttgggca
     1201 atcggcgtaa cggaaatgcg ctgattcaca atcggcacgc cgtatttggc agacagatat
     1261 tttgccgtcg ccaccaagtc tttgccgacc gtggtgattt tgttgtaaat gttttggttt
     1321 aacacgtcga tgtcggtgct gatgcagtcg tgcaaatcaa tgccgatggt aatggtgcgc
     1381 acgtcaaaat tctggtcggc caccattttg acggtttcta aaatctcgcc ggattgaata
     1441 ctcatggctc tccctccgac ttaaatacgg tgcatggctt ggaaaatttc ttcgttttgc
     1501 atacggatat caagcgcgag ttttttgctc tcttccgcaa acaaatccaa aacctcctgc
     1561 cgagatttgg ggcatttcga ggtatccacc aagataatca tggtaaaaaa atcgtccatc
     1621 agctgttggc tgatattaag gatgttgatt cggttttccg ccaaaatttt ggaaacatcg
     1681 tacacgatgc cgacgcggtc tttgccgatg acggtgatga ctgaattgtt cacaggctta
     1741 ctccttgcag atatccgtta aagtccgaaa ttataccacc gttggatttt gaagaaatat
     1801 tgtcaacaat atatacatac aaaatgccgt ctgaaactat ttcagacagc atcaagattc
     1861 agggttcgat taaataacca tccttatccc actgggtttt cctgaccaac ttgtcatcct
     1921 gataaacagc ctcgctcttt ttagaaccat cttcatacca ctccaaaacc accccgttgc
     1981 gttgatggtg gcggatagac agttccgaga gtaatcggcc gctttcatcc caagtcagaa
     2041 ttttggcagg ctcatcgttg accataacca tttccgtttt gatattgccg tcggcatacc
     2101 attgcttcca tacgccgttt gccttattct gtttaaactg gatttcgctt tccttgccgc
     2161 cgttacggta atagcggtac cccgtacctt cactcaaacc atttttataa ggcataacgg
     2221 cagatttttt accgttcgga taccagttga cccactcccc gtccggctta cccttgctga
     2281 agccccccgc catttttttc tgaccattaa aatgccacaa aatcaacata ccgttttgca
     2341 gggtaggcac aaaagatttg atttgcgttg aagcaacgat ataaggttcg gaatatttct
     2401 tcatcgacgg ataataaaaa tcctgcgcgt gcgcaatacc ctccgccaca ctatattgcc
     2461 tgatataagc ggcagaagac atcgtcgccg tcagtttccc gttctgatta aaataaacag
     2521 aataggtctg cgccggcaaa gcggccgaaa aacccaacag gacagttgaa aatacaatcc
     2581 gagataattt tttcattgca atagcgatat aaaaacaagg ctgtgtttta gtaatctttt
     2641 gatttcaatt atttgcaagg gaaaagacaa ttattttccg gttaggaata aacctattct
     2701 attgaatata ttgaagccaa gtacgcctat caacactata ttaaaacact gccaaaaaca
     2761 attaacttat aaacaatatg gtaaggattt ctctgccaag catcaaaccc gagacaacgt
     2821 atcgtaaaaa tgccgtctga aaacaaatcg tcttcagacg gcatttcccc ttcaactcac
     2881 tcttcaccca ataactgctc gcgcgtcaag aggaaaacaa aaccgtcgcc cccgctggtt
     2941 tccaaccaag taaaaggcaa ctccggatac gctgcttcca atacatccct gttatgcccg
     3001 atttccacca gcaatacacc tttgggattc agaaactttg ccgcattcag aagaatctgc
     3061 ctggtggcat ccaacccgtc cgccccgctg cccaatgcca atttcggttc gtgcaaatac
     3121 tcttcaggca ataactcaac cgattccgca tccacataag gcggattgga aacaatcaaa
     3181 tcataagtgc cttccaatcc gtcaaacaaa tccgtatgaa taagccggat gcgttcttcc
     3241 aaaccataat cttcgacatt aatccctgcc acttccaaag catccaagct cacatcaacc
     3301 gcatcaatgt gcgcatcggg atagtgatgc gccatctgaa tggcaaggca accgcttccg
     3361 gtgcaaagat ccaaagcatt atgcaccagc tcatcgtatt ctatccaagg acgaagtccg
     3421 ttacccaaca attcataaat aaaagaacga ggtatgatta cgcgctcatc cacatagaaa
     3481 tcaaactctc cctgccatgc ctggtgtgtc aaataagcgg ctggaatgtg ttcgacagca
     3541 cgacgctcaa taaccgccag cacttcctct ttttcagctt ccaagagttt tgcatcaaga
     3601 tatggggcaa gcatatccaa aggcaaattc aaagtatgca gaatcaaata agccgcttca
     3661 tcatgcgcat tatctgttcc atgaccaaaa aagagccctg cctcattaaa acggctgact
     3721 gcaaaacgta aaacatcgcg gatagtcgtc aattcttgtg ctgcctgatt aaacataata
     3781 tgaaccattc tgcgtataga tacttttaat tataacagaa acaacaagca aaccttttca
     3841 tatcgccaaa taaccaccca atctacccat acaactacat aaatgcccgc gcgaaaacca
     3901 tctcccgaac ggaaacggcg atggcggacg gtataggcaa tctgattggc tgggaaaaaa
     3961 cggggcttgt tgtcggtaag cagtggataa ccgcaaaaga cgacaaggtg tccgatgtct
     4021 gcaatgccaa cggcgagatg ggcgtaatcg ggctttacga gcctttctca cacggcgcat
     4081 tgacgatacc tggtcatccg aactgccgat gcgaggttgt ttccgtatcg ggtggcgaat
     4141 tgggggaatt tgccgaaaaa aaggagcttc gtaaagcggc tatgcagtat gcgcgggata
     4201 actttatcgg caaaagctat gtcaatgaaa acagcgggca tgaactgaag gtaacttggc
     4261 aaggtgtgaa acacgctgcg tcaaaggcaa atcaggcgga attatccatc ataacaaaac
     4321 ttgatgactt attgcgctac gcaaaatatg agggttctta ttcggataag aaaggtcatc
     4381 ctgatattat tgcagcacat aagtatcgtg ccgttgccaa ggttgggaat gagtctttaa
     4441 atatcggtgt gattgtaagg gaatttccag acggccataa acattacgac catttcatct
     4501 tgaaggatga gtaaagccct tttgcagtgt cgttctggag cggatagcgt taaggcaagt
     4561 acacttccag ccttacaaaa gggctttaaa ttcagcatgc catttataca ggcaggagta
     4621 aacccatgac aaagttatac gcagaaatcg ccaagaggga ggcgcaggac gacggcacgg
     4681 tcaaggtttt gggttatgcc tcaagcgagg cggtcgattc ggacggtgaa gtcatcgcga
     4741 cggcagctat gaaggcggcg attcccgatt atatgaagtt tgacgcgggg cgcgagatgc
     4801 acggctcaaa cgctgcggga acggcaattg aaatcaacgt tgaagacgac ggcataacct
     4861 ttttcggtgc tcatatcatc gaccccgttg tcgtgtcgaa agtcaaaaca ggcgtttaca
     4921 aaggcttttc catcggcggc agcgttaccg cccgcgataa tttgaacaag tcgcaaatca
     4981 cgggcttgaa gctgacggaa atcagccttg tcgagcgccc tgccaatccc gatgcggtgt
     5041 ctacctgctt taaggcggac aaaagtgcgg aagcggtaaa caacgataca gaacataatg
     5101 ctacatattt tagccatttc ccttccaaac aaaaaagcac cggcggcagc cgatgcccct
     5161 tcctttacag gttcccctat tttttcactg cgggcagcac tggtttagc
//
LOCUS       DAWFJR010000116         4895 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWFJR010000116 DAWFJR010000000
VERSION     DAWFJR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.116"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(612..1148)
                     /locus_tag="WGR04_001892"
     CDS             complement(612..1148)
                     /locus_tag="WGR04_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ0795390.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(1182..1538)
                     /gene="folB"
                     /locus_tag="WGR04_001893"
     CDS             complement(1182..1538)
                     /gene="folB"
                     /locus_tag="WGR04_001893"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ0795391.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            1594..2196
                     /gene="plsY"
                     /locus_tag="WGR04_001894"
     CDS             1594..2196
                     /gene="plsY"
                     /locus_tag="WGR04_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ0795392.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLIAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHLSWIWATLLIALLVLFRHKSNISNLLKGKEGKIGEKR"
     gene            2284..3129
                     /locus_tag="WGR04_001895"
     CDS             2284..3129
                     /locus_tag="WGR04_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ0795393.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNKKDKPYWYIDTHGG
                     AGLYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKELISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            3375..4349
                     /locus_tag="WGR04_001896"
     CDS             3375..4349
                     /locus_tag="WGR04_001896"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ0795394.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTLGHGVYVFTLNTENEFVLTKENPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPAKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVFMGSSEEVAYLDRLHAK"
     gene            complement(4492..4710)
                     /locus_tag="WGR04_001897"
     CDS             complement(4492..4710)
                     /locus_tag="WGR04_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ0795395.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         1273 a         1451 c         1004 g         1167 t
ORIGIN      
        1 aagtattcat atattctatc tttaattatt tcattatttt cttcatctaa aaatatacat
       61 ctttttaata gttccccatt aaattcataa acatcgtaat aagctttaaa aatagatatc
      121 atatcatttc cttatcttcc ataaatcggc ttcggttgtt taaataccgc ccgtgccaat
      181 taccgttctc aaattcaaat tgggctttta ggtctataac aaagaaaaag ccttggcagg
      241 ataaacctac caaggctttt tattaaaaaa aacgctctat tactgtgtct aaaaatgcaa
      301 aaaaagtaac catcataacc cttttgccaa aatcaatatc atcatccgat gaatgaattg
      361 acggtatata agaaagaatt aataagctca taaaccggta agaaaaatca gtaacaaaga
      421 tattcattat taactattct taaaaatgat cacgttcgtt tgggtaatta tcagcccata
      481 ccatacccta ctcctattac taaagcacga atgactttaa aggacgcgcc cagagacaag
      541 ccgaaaaggc atatctcatc cgccaaacaa aatgcccgcc gacttactcc ggcaggcagt
      601 tcaacaccct gtcaatcctt catcagccag tattgcaaac cgattaatgt cttgccgtct
      661 ttaatttcat cgtttgccaa tgcctgacgg acttcttctt tcgacatcaa tacggtttcc
      721 gtaatctcgt cttcgtcatt ggcaagcgta ctgcccaagc gcacgccttc cgcctcgaac
      781 agatacattt tttcattgca aaaaccgacc gccgtataaa agctgtacag caggcgcacg
      841 ctgtcggcaa cataaggtgt ttcctccgcc aattctcgca gcgcacacgc tgccatatcc
      901 tcgcctgcca catccagctt gcccgcagga agttccaagg tcgcctgatt tgccgcataa
      961 cgccactgcc gcaccaaaac gaccttttca tcatccgtta ccgccaacac gcacgccgcg
     1021 cccggatgac ggatgacgat acgctgccct tcattgccgt tgggcaacct gaccttatcc
     1081 ctgctgatac tgacgaaacc gccctcgtaa atggtttcgc cgcctaattt tacttccctc
     1141 aaatccactt catatctcct ttccgtttcc tattctgccg ttcaatcttc acgcttgccg
     1201 cgctcgattt ccacgccaac ctcgcgcacg ccttccaaaa tacccggctt gacgattttc
     1261 acgcgcaccc acaccgcgcc gaaatatccc aaaaccaaat cggcaatata ttccgccaac
     1321 gcttccaaaa gcaggaaatc ctgttctttc agatgtcggc gcaatgtttc gcatacctcg
     1381 gcataatgca ccgtattggc aatatcgtcg tccgaacccg ctttctcggg aacgccgatg
     1441 tccaaatcga caatcagggt ctgcaaccgt tcgcgttccc agccgtacac gccgataagc
     1501 gtatccgcct tgatgccgtg caaaaagatt ttatccataa tccgccccgt tttttgctaa
     1561 aataaccgca ccattctaag taaagtacca caaatgttca acataccggc tgttgccgtt
     1621 tcctatctga tcggctcact ttctttcgcc gtcatcgttt ccaagtatta cgggatggac
     1681 gacccgcgca cctacggatc gggcaatccc ggcgcgacca atgttttacg cagcggcaaa
     1741 aaaaaggcag ccgcgctgac gctcttgggc gatgccgcca aaggtttgat tgccgttttg
     1801 cttgcacgcg tgcttcaaga accgctcggt ttatccgaca gcgcaatcgc tgccgtcgca
     1861 ctcgccgcgc tggtcgggca tatgtggccg gtgtttttcg gatttaaggg cggcaaaggc
     1921 gtggcaacgg cattgggcgt gcttttggca ctctctcccg caactgcctt ggtctgcgcg
     1981 ttgatttggc ttgttatggc attcggcttc aaggtgtcct cccttgccgc attaaccgcc
     2041 acaatcgccg ccccccttgc tgcgctgttt tttatgcccc acctttcttg gatttgggcg
     2101 acgctcctta tcgccctgct ggtgttgttc cgccacaaaa gcaatatcag caacctgctt
     2161 aaaggcaaag aaggcaaaat cggcgaaaaa cgctgattcg gcatttgttt tccattacgt
     2221 catgccgtct gaaaccatat ttccgcgttc agacggcatt tccgcacaaa cggctttcat
     2281 accatgctca gttacagaca cgcattccac gccggcaacc acgccgatat gctcaaacat
     2341 ttcaccttgt ttttagtgct gcaatatttc aacaaaaaag acaagcccta ctggtacatc
     2401 gacacgcacg gcggcgcggg tttgtacaat ctcgaaggca gcgaggcaca aaaagtcggc
     2461 gaataccggc aaggcatcgc cctgctccga caggcgcaaa acctgcctgc cgaactctct
     2521 gactttgccg cgcacataca aaaaatcctg ccctcgcccg aactttactg cggttctccg
     2581 tggctggcgc aatcgctgac ccgcgtcggc gacaaattac gcctgtttga gctgcacccc
     2641 accgactttg tccatctgca aaacaatatg ggcgaagcag gattgggcaa acgcggacaa
     2701 gtgttgcgcg aagacggtta caaagagctg atttccctgc tgccgccccc gccgcgccgc
     2761 gccaccgtcc tcatcgaccc gccctatgag gaaaaacagg actaccggcg tgtaacggaa
     2821 acgctgaaag ccgctttaaa acggtttgag tccggctgtt atctcatttg gtatccctgc
     2881 ctcagccgcg aagaaagccg caagctgccc gaagaattga aaaaactcct gcccgacaac
     2941 taccttcacg ccgaacttca cgtccacgcg ccgaaagccg acggtttcgg tatgcacggc
     3001 agcggaatgt tcgtcatcaa tccgccctat ctgttggcgg aacaattggc ggcaaacctc
     3061 cccgccctga cgcggctgct ggcgcaagac gaaggcgcgc gctacctttt ggacagcaaa
     3121 atccgctgac cccgatgccg tctgaaaccc ttcagacaac atctcccgtc aaataaacaa
     3181 aaccggacga tataccggca gtcggcactt ttaagaataa atatcccctc atccgcctgc
     3241 cgcgttccga cttgccccat gccgtttttt tacactacaa tcccctagac tatcctgcaa
     3301 aacgcaccaa aggaatacgc cacaataacc tgccgccttt cagacggcac aaacctttcc
     3361 gaaacgagca aactatggac acactgaccc gattcctccc cgaacacctg caacaaaacc
     3421 agctgcccga agcactcggc ggcgttttac tgtccgttgt ttccgcctgc accgaaatca
     3481 acgccaaagt ccgcctcggc gcactggccg gcgtattggg tatggcgggc acgggcaata
     3541 ttcagggcga agaccaaaaa aaactggatg ttatcgccaa caacatcatg attgacacac
     3601 tcaaagccaa ccctgccgtt gccggtttgg caagcgagga agaagactct ttcgtaagtg
     3661 ccggtgaaaa cggacgctat ctcgtcctat tcgacccttt agacggatcg tccaatattg
     3721 atgtcaacat ttccgtcggt acgattttct ccatccttga aaaacccgaa ggcgcattgg
     3781 caaccgaatc attccttcaa acgggcagac agcagcttgc cgcaggctat gtcctctacg
     3841 ggcctcaaac ccagctcgta ttcacactcg gacacggcgt gtacgtgttt acactcaata
     3901 ccgaaaacga atttgtcctg accaaagaaa acccgaaagt acccgaaagt accaaagaat
     3961 tcgccatcaa tatgtccaac cgccgccact ggctgccccc cgtccaacaa tacatagacg
     4021 agctcttggc gggcgaaact ggcacgcgcg gcaaaaacta caatatgcgc tgggtagcca
     4081 gcatggttgc cgaaatccac cgcatcctga tgcgcggcgg cgtgttcatg tatccgcaag
     4141 acaaacgcga ccccgccaag cccggcaaac tgcgcctgat gtacgaagcc aaccctatga
     4201 gcctgatact cgaacaggcg ggcgcatcgg caagcaacgc atatcaagcc atgctcgaca
     4261 tacagcccga aaacctgcac caacgcgtcg ccgtcttcat gggcagcagc gaagaagtcg
     4321 cctatctcga ccgcctgcac gcaaaataga cagacagccg ttcacacaag tacacggcgg
     4381 cggcaatccg ataccgccgc cgttatttca aacagcacaa gccttccggt caaatgacaa
     4441 aaatgccgtc cgaacacatt tcagacggca tttcaaaaac tcaaacccga attattttcc
     4501 cgctgctttt tgcaggatgg cgtacaactc gtctttcagt ttcaatttgg cttttttcag
     4561 ctcatcgatg gtttccgcac cgctggtaac cggattgttg accagaccgg taattttatc
     4621 gtccagctcg ttgtgttcgt cgaacagacg ggcgaagcgg gaattttcct gtttcaattt
     4681 ggaaatcaaa tcacggtatt ctgggaacat atcgctttcc tctcttggtt tacggttaat
     4741 aaagtcaaac tgaatcttta cattaattat aacaaagata atatttttat ccagtatttt
     4801 atcggatacg gcaaaatttt cttttaacac cgccgtccgg ttgataaaaa cgttgacaat
     4861 atcacgcttt acgcataaat tttcagcctt tccta
//
LOCUS       DAWFJR010000117         4723 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWFJR010000117 DAWFJR010000000
VERSION     DAWFJR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.117"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..194
                     /locus_tag="WGR04_001898"
     CDS             <1..194
                     /locus_tag="WGR04_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0795396.1"
                     /translation="NNRINQTEEDNYSTGVGSCINGFPVRYYPFIREKQQLTQQELVG
                     YHQQVEQLVQSFVNNPSKK"
     gene            211..486
                     /locus_tag="WGR04_001899"
     CDS             211..486
                     /locus_tag="WGR04_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795397.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADNAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLMCISVFWIVVFNL"
     gene            complement(573..1706)
                     /locus_tag="WGR04_001900"
     CDS             complement(573..1706)
                     /locus_tag="WGR04_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795398.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNTFVAKLARLFRNADRAVVIVKESMRTEESLAETADDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYYIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTVHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLEDLEKEVRRYAEAAARRSGGRRDLSY"
     gene            complement(1977..2075)
                     /locus_tag="WGR04_001901"
                     /pseudo
     CDS             complement(1977..2075)
                     /locus_tag="WGR04_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2111..2488)
                     /locus_tag="WGR04_001902"
     CDS             complement(2111..2488)
                     /locus_tag="WGR04_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ0795399.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGTIIEFLMVPNWKLFEIKASPWLPDSVGINERYERLK
                     TMLRYIFTEKDIVNVQFDYYNKK"
     gene            complement(2519..2917)
                     /locus_tag="WGR04_001903"
     CDS             complement(2519..2917)
                     /locus_tag="WGR04_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ0795400.1"
                     /translation="MCEFKDFRRNIPCFEEYDENSFIGKWYDDGVWDDEEYWKLENAL
                     IEVRKKYPYPMDIPRDIVIGIGTIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFR
                     VMLRYIFTDLDVEDWKFFYFPIQHSKGRLR"
     gene            complement(2923..3600)
                     /locus_tag="WGR04_001904"
     CDS             complement(2923..3600)
                     /locus_tag="WGR04_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hint domain-/BECR-fold containing toxin"
                     /protein_id="HEZ0795401.1"
                     /translation="MVKTADGYKAIAHIQTGEHVFAKDETSGKTGYKPVTARYGNPYR
                     ETVYIEVSDGIGNSQTLISNRIHPFYSGGKWIKAEDLKAGSRLFAESGKTQTVRNIIV
                     KPKPLKAYNLTVADWHTYFVKGNQAETEGVWVHNACPPRKTPSTPVYGNDSEAYAAAK
                     KLGYRKIKERTRNDAAIFKKGKSYISRDVDSHNGGAWKEASSPKNLNRKETRNGTFDK
                     NLNRIGD"
     gene            complement(3736..4281)
                     /locus_tag="WGR04_001905"
     CDS             complement(3736..4281)
                     /locus_tag="WGR04_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ0795402.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAEWVEKKGWNAFE
                     QFLLDNEVGNLSDMNRKPFTW"
     gene            complement(4268..4420)
                     /locus_tag="WGR04_001906"
     CDS             complement(4268..4420)
                     /locus_tag="WGR04_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795403.1"
                     /translation="MDAKPLGGNIKMPLYFSDGISVNKKVGIGAQNSLHLAGIGMKSL
                     RKNDYS"
     gene            complement(4402..4674)
                     /locus_tag="WGR04_001907"
     CDS             complement(4402..4674)
                     /locus_tag="WGR04_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795404.1"
                     /translation="MMSVKELFKVILDENKDFPIRTIHRTPIGILPKPVALSIVQYED
                     DQGFYLFYLDETGQEQTDTYHDTLDSAFEQVEFEFGISKEEWMQSP"
BASE COUNT         1314 a         1091 c          849 g         1469 t
ORIGIN      
        1 ggaataatcg aataaaccaa actgaagaag ataattacag cactggcgta ggttcctgta
       61 ttaacggttt cccggtacgg tattacccgt ttattcggga aaagcagcag ctcacacagc
      121 aggagttggt aggttatcac caacaagtag agcaattggt acagagtttt gtaaacaatc
      181 caagtaaaaa ataatttaaa ggatcttatt atgaatgagg gtgaagttgt tttaacacca
      241 gaacaaatcc aaaccttgcg tggttatgct ttccgtggcg atacctatgg cggttggcgt
      301 tatttggcta atttgggtga ccgttatgcg gataatgctg ctgcaattgt cggtaaggat
      361 gcaaacttaa atggtttgaa tttatggatg aaaaaagggg tggaaaacct atgggatgat
      421 acggtcggta aaaagacccg tttaatgtgt atttccgttt tttggattgt ggttttcaat
      481 ttgtagtgaa tcggattcgg catatacggc attgcaaaaa gcgtttgact ctccaatgcc
      541 gtctgaaaac cggtttcaga cggcatttgc gttcagtaag aaaggtcgcg cctgccgccc
      601 gaacgtctcg ccgcagcctc tgcataacgg cgcacctctt tttccaagtc ttccaagttc
      661 aaaggaaaat caggcagtct gtctccctgt ttctcttcgc ggataatccg cccaccatcc
      721 aaataccacg tctgttgcgc atgataggtc tgtatatccg ccgttacgcc atccgctttc
      781 aatgctaccg tcgaagattg tgcaataaaa agatttccgt ttttcaaata atattcgaaa
      841 ctctggcgtt ttttcccatt gtcgaaactc caatagactt tttgcggcag accgtccgca
      901 tcatagccga ccacaagact gttcgccttc atcccccgtg gcatcaattc ccgcatattc
      961 tgataaaaca cagaatcgcg cgagtccgac gcaatccggt tactctcttc gcggaagtcc
     1021 caaaccttct gctcatcatt cgcgacatcc cgatatttcg ccaaatatac ctgggccatc
     1081 tgataacacc cgaggcaatg ctcataaaca tcttccccga ttttcccgcg ccccgacgca
     1141 tcaaataccg aaccgtccgg ttgccaaaca acccgatatt ctcctgtcgt ttcataattt
     1201 tccccgtgaa ccgttccgcc gtacacattt acagaaaacg gacgatcgtt ccgatacaga
     1261 tattcggcat taacaaacgc ttccggcgag cgttgcaaaa gcgaaaccgc aaccaaaccg
     1321 ccctcgccga tatagtaatc cagccaaacc tcttccccat gttcctgctc cgttacgtga
     1381 aaccatttcg ccttttcttt caaacgactg agccggatag cgagcgcaag ataatccttt
     1441 tccgactgca acggaccgtc atccgcagtt tcggcaagac tttcctccgt tctcatcgat
     1501 tccttcacga tgacaaccgc cctgtcggca tttcggaaca ggcgggcaag tttcgccaca
     1561 aaagtattcg gattttcagg tatttcagtt gccgtatcgc tcaaaaacca acgcggatta
     1621 atctcatagg caatacccgt ccccagccaa aaggcaaata caagtgcaaa aaatgacaac
     1681 aataccggtt tgaatttttt aaacatattt atttttcgtt taaaaggata tatcgattat
     1741 atcagactag ctttgattgt ttgattttta atctaatcta cgcttgctgc cgtactttaa
     1801 aaaaacctat gcaggctgcg gcttgttact cttgtttgct ttgattcaaa ttttcaacta
     1861 attcaaatgc ttaagatcga tattgccttt gccatttttc ccaatttaac ggtcttcccg
     1921 agcgttccca atcttctaaa actgatattt tgaacgtttc catttcccac caatattaag
     1981 tcttctgcct taatccattt gccgtccgaa taaaacggat ggatgcggtt ggaaatcagg
     2041 gtttggctgt taccgatgcc gtctgaaagc cgaataccgc ttcagacggc attttgattg
     2101 ccgggttttg ctattttttg ttgtaataat caaattgcac gttgactatg tctttctcgg
     2161 taaaaatata acggagcatt gttttaagcc tttcataacg ttcgttaatt cccacgctat
     2221 caggtaacca aggggaagct ttaatttcaa aaagtttcca atttggaacc attaaaaact
     2281 caataatggt accgattcca atcacgatat cccttggtat atccatcgga taaggatatt
     2341 ttctcctaac ctcgattaaa tcattctcca acttccaata ttcttcatca tcccacaccc
     2401 cgtcatcata ccatttgcca ataaatgaat tttcgtcata cccctcaaaa taaggaacgt
     2461 ttcttataat atccttgaac tcacacataa taatgtatct ccaatataat taaacttttc
     2521 atctcaatct acctttacta tgttgtattg gaaagtaaaa aaatttccag tcctctacat
     2581 ctagatcagt aaaaatataa cggagcatta ctctgaacct ttcataacgc tcattaattt
     2641 tgacactttt aggcaaccaa ggagaagctt taatttcaaa aagtttccaa ttttgaacca
     2701 ttaaaaaatc aataatggta ccgattccaa tcacgatgtc ccttggtata tccatcggat
     2761 aaggatattt ttttctaacc tcgattaaag cattctccaa tttccaatat tcttcatcat
     2821 cccacactcc gtcatcatac catttgccaa taaatgaatt ttcgtcatac tcttcaaaac
     2881 aagggatgtt tcttctaaaa tccttgaact cgcacataat aattaatctc caatacgatt
     2941 taggttttta tcaaatgtac cgtttcttgt ttcttttctg ttcagatttt tgggtgaaga
     3001 tgcttctttc caagcccctc cattatgtga atctacatcg cgtgatatat aactctttcc
     3061 ttttttaaaa atagcagcat catttctcgt tctttctttt atttttctat atcccaattt
     3121 ctttgctgct gcatatgctt ctgaatcatt cccatatacg ggggtagatg gtgtttttct
     3181 tggcggacac gcattatgaa cccaaacccc ttccgtttcc gcctgattac ccttgacgaa
     3241 gtaggtatgc cagtcggcaa cggtcagatt gtaggctttg agcggttttg gtttgacaat
     3301 gatgttgcgg acggtttggg ttttgccgct ttcggcaaac agcctgcttc ccgctttcaa
     3361 atcttccgct ttaatccatt tgccgcccga ataaaacggg tggatgcggt tggaaatcag
     3421 ggtttggctg ttgccgatgc cgtctgaaac ttcaatgtaa acggtttccc gatacggatt
     3481 gccgtatcgg gcggtaacag gtttgtatcc agtttttcca ctggtttcat ctttggcaaa
     3541 gacgtgctct ccagtttgga tatgtgcgat ggctttgtag ccgtctgccg ttttgaccaa
     3601 ggtgctgccg tggaaggagc aggtgtagga tttttgaaga acttagttcc caatatcctg
     3661 tttaaaataa ggggaaggtc gcctgaaaat taaacacggt ttcagacggc actttgatta
     3721 aatttaagaa aatccttacc aagtaaacgg tttccgattc atatccgaaa ggttgccaac
     3781 ttcattatcc agcaagaact gctcaaaagc attccaaccc tttttttcca cccattcggc
     3841 ttcctgttta tacaagggga caagcaaagg gaaaacgata ttgtagtgtt cgccataaca
     3901 gacctgaaaa tcatcgtcga aataaaatgg ggcggaaaca tacagtgcat ccattttgct
     3961 accttcgata attaccctgg gtaattgaat aatttcacct cttaaaatag gttgtctatt
     4021 gtcaatcaaa tattcggcta ctcctgatag aaaagctgca atattttctt tattccattc
     4081 ttcagaacag gtaaaaatta attcaaacct actcttatag ttcaagtcat atcgattcaa
     4141 gcctaaagta gaaaaagtag ttaccccttt atatggttgt gatgggaaga cggaaacgct
     4201 gagatgataa cgtcgatcca agcattttat accgctttcg atttcaccca aataatgttc
     4261 taaatgttca actataatca ttttttctta aagatttcat ccctatcccg gcaagatgta
     4321 ggctgttttg ggctccaata ccgacttttt tattgacgga aatgccgtct gaaaaataaa
     4381 gcggcatctt gatattgcca cctaagggct ttgcatccat tcttctttgc taattccaaa
     4441 ttcaaactcc acctgttcaa atgccgaatc taatgtgtca tgataagtat ccgtttgttc
     4501 ctgaccggtc tcatccaaat aaaacaaata aaatccttgg tcatcttcat attgaacaat
     4561 acttaaagca acaggtttgg gaagtatccc tattggcgtg cgatgaatcg ttctaatagg
     4621 aaaatcttta ttttcatcta agatgacttt gaataattct tttacactca tcattcttca
     4681 atcccgaata attgttaccg cactattttc tattacacgt cca
//
LOCUS       DAWFJR010000118         4675 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWFJR010000118 DAWFJR010000000
VERSION     DAWFJR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.118"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..690
                     /locus_tag="WGR04_001908"
     CDS             <1..690
                     /locus_tag="WGR04_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEZ0795405.1"
                     /translation="ALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAESVTKAESHKV
                     SVAIAIVVIFGTLAIFTYPLFYTWSQDLINAHQFGIYVGSSVHEVAQVYAIGENIDPI
                     VANTAVISKMIRVMMLAPFLLMLSWLLTRSDGVSENTSHKITIPWFAVLFIGVAIFNS
                     FDLLPKELVKLFVEIDSFLLISSMAALGLTTHASAIKKAGLKPFVLGILTYLWLVVGG
                     FLVNYGISKLI"
     gene            715..1134
                     /locus_tag="WGR04_001909"
     CDS             715..1134
                     /locus_tag="WGR04_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ0795406.1"
                     /translation="MAQLPLYLTSEIKDFTVGTPEVLQSFFEYVPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNYVLILWDDACTIAALCINDYIHAVYDFVAQ
                     AGYCRNGFPEAQGEWLKVADRVLDDELLDKILSRKPT"
     gene            1156..1662
                     /gene="luxS"
                     /locus_tag="WGR04_001910"
     CDS             1156..1662
                     /gene="luxS"
                     /locus_tag="WGR04_001910"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ0795407.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVANVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            1808..4603
                     /gene="polA"
                     /locus_tag="WGR04_001911"
     CDS             1808..4603
                     /gene="polA"
                     /locus_tag="WGR04_001911"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ0795408.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSSETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLAGVMEHAS
                     EIKGKVGENLRAALPRLPLSYDLVTIKTDVDLHAELSDGIESLRRTTPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEMPSEKQAEKATVPERLDYQA
                     VTTEAQFAALLDKLAQADTIGIDTETTSLDAMNAALVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQYVLGRLKPHLGNPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSGRWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QQAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMRRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSHWLDSAGLKTKLIMQVHDELVLEVPEAELDLVKEKLPQIMAKVDEGMLK
                     VPLVAEVGVGENWEEAH"
BASE COUNT         1198 a         1253 c         1182 g         1042 t
ORIGIN      
        1 gcacttttag gcattcgtta tctaaaaatg gataaacaat tggtttatct cactggggca
       61 ggttgcagca tttgcggtgc ggcagcggtt atggcggcag agtctgttac caaagcagaa
      121 tcccataaag tttcagtggc gattgccata gtggtcattt tcgggacgct tgctattttt
      181 acttacccct tgttctacac gtggtcacaa gatttaatta acgcccatca attcggtatt
      241 tatgttggtt ctagtgtaca cgaagtggct caagtgtatg cgattgggga aaatattgat
      301 cctatcgtgg cgaatactgc cgtcatttcc aaaatgatcc gagtgatgat gctcgccccc
      361 tttttattaa tgctttcttg gttattaaca cgtagtgatg gggtatcaga aaatacctca
      421 cacaaaatta caattccttg gtttgctgta ctttttattg gtgttgccat ttttaattct
      481 tttgatttat taccaaaaga actcgtgaaa ttattcgttg aaatcgattc tttcttatta
      541 atttcatcaa tggctgcgct tggcttaacg acgcacgcaa gcgcaatcaa aaaggcagga
      601 ttaaaaccgt ttgttttagg aatactaact tatttatggc tagtggttgg tggattttta
      661 gtgaactatg gaatatcaaa attaatataa aattcactaa agagagcgta cccaatggca
      721 caattaccgc tatatctgac ttctgaaatc aaagacttta ctgtcggcac gcccgaagtt
      781 ttacaatcat ttttcgaata tgtcccttat ggcgtcgtct ttgaagacga cggcgacaca
      841 ggctacttct atgccgcttc gcaagacggg attctagatg ccttgcacat ctacaatgtt
      901 gaagatgtat ccgacaaaca tatccccaat tatgtcttga ttttatggga tgatgcctgc
      961 accatagccg cattgtgtat caacgactac attcatgccg tctatgattt tgtcgcgcag
     1021 gcaggatatt gccgcaacgg cttccctgaa gcacaaggcg aatggctgaa agtcgcagac
     1081 cgcgtcttgg acgatgaatt gttggacaaa atcctatccc gaaaacctac ataacccact
     1141 caaaaggata acaaaatgcc cctactagac agtttcaaag tcgaccacac ccgtatgcac
     1201 gcccctgccg tacgcgtggc aaaaactatg accacgccca aaggcgacac cattaccgtg
     1261 tttgacctgc gtttctgcat tcccaacaaa gaaatcctgc ccgaaaaagg catccacacg
     1321 ctggagcatt tgttcgccgg ttttatgcgc gatcacttga acggcaacgg cgtggaaatc
     1381 atcgacattt ccccgatggg ctgccgcacc ggtttttata tgagtttgat tggtacgcct
     1441 tccgagcagc aggttgccga tgcgtggcta gcttcgatgc aggatgtggc caatgtcaaa
     1501 gaccaaagca aaatccccga gttgaacgaa taccaatgcg gcacttacca aatgcactcg
     1561 ctcgccgaag cgcaacaaat cgcgcaaaat gtgctggcgc gcaaagtggc ggtgaataaa
     1621 aacgaagagc tgacgctgga cgaagggctg ctgaacgcct aatccgccaa aaatgccgtc
     1681 tgaacaaggg tttcagacgg catttgcctt ttccgttata atccggggtt gtgcggggtc
     1741 gggttttaag ccggcatcgt ccttccctat ttttttctgt cccttatcgg ttttaagcgg
     1801 gttttttatg tccaacagac ctacactcct cctcgttgac ggctcgtcct acctctaccg
     1861 tgcgtatcac gcgatggggc aaaacctgac cgcccccgac ggcgcgccga cgggtgcgct
     1921 gtatggcgta ttgaatatgt tgcgccgttt gcggtcggaa tatccgcacg attattgcgc
     1981 ggtggttttt gatgcgaaag gcaaaaattt ccgccatcaa atgtttgaag aatacaaggc
     2041 gacgcgcccg ccgatgcccg acgatttgcg cccgcaggcg gaagcactgc cggatttagt
     2101 gcgcctgaca ggctggccgg tattggtgat tgggcaggtg gaggcggacg atgtgatcgg
     2161 cacgctggcg aaacaggggg cggaacatgg tttgcgagtc attgtttcga ccggcgataa
     2221 ggacatggcg cagttggtgg atgagcgcgt tacgctggtg aacacgatga gcagcgaaac
     2281 gctggatatt gaaggcgtga aggcaaaatt cggcgtgcgc cccgaccaaa tccgcgatta
     2341 tctcgcgctg atgggcgaca aggtggacaa cgtgccgggc gtggaaaaat gcggcccgaa
     2401 aacggcggtg aaatggctgg aagcctacgg ttcgctggct ggtgtgatgg aacacgcttc
     2461 ggaaatcaag ggcaaagtgg gcgagaatct gcgtgccgcc ctgccgcgcc tgccgctgtc
     2521 gtatgactta gtaacgatta aaaccgatgt ggacttgcac gccgagcttt cagacggcat
     2581 cgaaagcctg cgccgtacta cgccgaaatg ggcgcagctg gttgtcgatt tcaaacgctg
     2641 gggcttccgc acctggctga aagaagcgga atcaaacatg aataccggct cgaccgatga
     2701 tttgttcggc agcgacagca tcggcgagca ggcggctttg aatgcggaaa tgccgtctga
     2761 aaaacaagct gaaaaagcca ccgtccccga aaggctggat tatcaagccg ttaccaccga
     2821 agcgcagttt gccgctttgt tggacaaact ggctcaggcg gacacaatcg gcatcgatac
     2881 ggaaaccacg tcattagacg cgatgaacgc cgcgctggtc ggcatcagca ttgcgttcca
     2941 agcaggggag gcggtttaca tccccgtagg acacagcctg accgccgcgc ctgaacagct
     3001 tgatttacaa tacgtattag gccgtctgaa accgcatttg ggaaaccccg ccctgaaaaa
     3061 aatcgggcaa aacctcaaat acgaccaaca cgttttcgct aactacggca tcgccctgaa
     3121 cggcattgcc ggcgacgcca tgctcgcttc ctacatcatc gagagccatc tcggacacgg
     3181 attggacgaa ttgtccggac gctggctcgg cttggaaacc attacctacg aatcgctgtg
     3241 cggcaaaggc gcgaagcaaa tcggttttgc cgatgtcgcc atcgggcagg cgaccgaata
     3301 cgccgcccaa gacgccgatt tcgccctgcg cctcgaagcg cacttgcgcg cgcaaatgga
     3361 cgaaaaacag cttgaaatgt atgaaaaaat ggagctgccc gtcgcgcagg tattgtttga
     3421 aatggaacgc aacggcgtgc aaatcgaccg cgccgaactc gcccgccaaa gcgcggaact
     3481 cggcgccgag ctgatgaagc tcgaacaaca agcctatgcc gccgcaggcc agccgttcaa
     3541 cctcaattcg cccaaacagc tgcaagaaat cctgttcgac aaaatgggca tccccaccaa
     3601 aggcctgaaa aaaaccgcca aaggcggcat ttccaccaac gaagccgtgc tcgaacagct
     3661 cgcgcccgac taccccctgc ccaaaatcat cctgcaaaac cgcagcctgg cgaaactcaa
     3721 atccacctac accgacaaac tgcccgaaat gatttcgccc cgggataacc gcgtgcatac
     3781 cacctacgcc caagccgtcg ccattacagg acgcttggca agcaacaacc ccaacctgca
     3841 aaacatcccc atccgcaccg ccgaaggccg ccgtgtgcgc cgcgccttta ccgcaccgca
     3901 aggcagcgtc atcgtttccg ccgactattc ccaaatcgag ctgcgcatta tggcgcacct
     3961 ctccggcgac aaaaccctca tcgccgcgtt ccaaaacggc gaagacgtac accgccgcac
     4021 cgccgccgaa gtgttcggca cggcgcccga aaacgtctcg tccgagcaac gccgctacgc
     4081 caaaaccatc aacttcggct taatttacgg tatggggcaa tacggtttgg caaaatcatt
     4141 gggcatcgac aacctttccg ccaaaaactt tatcgaccgc tacttcgccc gctaccccgg
     4201 cgtcgccgaa tacatgcggc gcaccaaaga acaagccgcc gcccaaggct acgtcgaaac
     4261 cctgttcggc agaaggctct atttgcccga catccgcaac aaaaacgcca acgcccgcgc
     4321 aggagccgag cgcgccgcca tcaatgcccc catgcaaggc accgcgtccg acctcatcaa
     4381 acgcgccatg atagacgtgt cccattggtt ggattcagcc ggcctgaaaa ccaaattgat
     4441 tatgcaggtg catgacgaat tggtgttgga agtacccgaa gccgagctgg atttagtcaa
     4501 agaaaaactg ccgcagataa tggcgaaagt ggatgaaggg atgttgaaag tgccgctggt
     4561 ggctgaggtt ggcgtagggg agaattggga agaggcacat tgattgaaag gtgttatatg
     4621 ctatctttat ttaaataaaa tttaattttt ggtatatttt ttctaaatgt tccta
//
LOCUS       DAWFJR010000119         4588 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWFJR010000119 DAWFJR010000000
VERSION     DAWFJR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.119"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..127)
                     /locus_tag="WGR04_001912"
     CDS             complement(<1..127)
                     /locus_tag="WGR04_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ0795409.1"
                     /translation="MKEYKVVIYQESQLSSLFFGAAKVNPVNFSAFLNKQTPEGWR"
     gene            complement(202..1545)
                     /gene="argG"
                     /locus_tag="WGR04_001913"
     CDS             complement(202..1545)
                     /gene="argG"
                     /locus_tag="WGR04_001913"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ0795410.1"
                     /translation="MSQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEVKPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKK"
     gene            1872..2012
                     /locus_tag="WGR04_001914"
     CDS             1872..2012
                     /locus_tag="WGR04_001914"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795411.1"
                     /translation="MQTAALKQTLPEKRKLDPLPNPLPWEREQITAPSTFSDGIGKET
                     HL"
     gene            2009..2809
                     /locus_tag="WGR04_001915"
     CDS             2009..2809
                     /locus_tag="WGR04_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ0795412.1"
                     /translation="MNAFNLTIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALDLPVVRHPEAAKFIEG
                     VTRKRGEPLDSPEHAQRLKMADFPQGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLDTYYVGRFNQTKRGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            2849..3835
                     /locus_tag="WGR04_001916"
     CDS             2849..3835
                     /locus_tag="WGR04_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ0795413.1"
                     /translation="MQYRIRRENEAPEAKNTGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            4492..4588
                     /locus_tag="WGR04_001917"
                     /pseudo
     CDS             4492..4588
                     /locus_tag="WGR04_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         1063 a         1179 c         1278 g         1068 t
ORIGIN      
        1 cccgccagcc ttcgggggtt tgtttgttga ggaacgcgct gaaattgacg gggttgacct
       61 ttgccgcgcc gaaaaacagg ctggacaact ggctttcctg ataaatgacg actttgtatt
      121 ctttcatagg attcctttta acataaggga tcatcttttc agacgacccc ttaagttgct
      181 aatgatgcaa aacagcaaac cttatttctt attgcccaac tgcggtaata ccgagccttc
      241 gcccagcgac agcaaaccgc tttgcgagta gatgcccagt ttggcgcggg tgtcggtgat
      301 gtcgaggttg cgcatcgtga gctgtccgat gcggtcgagc ggggtaaacg ccgcaccttc
      361 gactttttcc atactcaggc gttcgggttg gtaggtcagg ttgggcgatt cggtgttcag
      421 aatcgagtag tcgttgccgc gccgcagttc gagggtgact tcgccggtaa cggctttggc
      481 gacccagcgt tgggcggttt cgcgcaacat caaggcttgg ctgtcgaacc agcggccttg
      541 gtagagcaaa cggccgaggc gcaggccgtt gatgcggtat tgttcgatgg tgtcttcgtt
      601 gtggatgccg gtcaccaagc gttcgtaggc gatgtggaac aacgccattc ccggggcttc
      661 gtagatgccg cgcgatttgg cttcgatgat gcggttttcg atttggtcgc tcatgcctaa
      721 gccgtggcgg ccgccgatgc ggttggcttc gaggaagagt tcgacgggat cggcgtattc
      781 tttgccgttc agtgcaaccg gcacgccttc ttcaaagcgc acgctgactt cttcgggttt
      841 gacttcgacg ttttcgtccc agaaggcaac gcccatgatg ggtttgacga ttttgatgcc
      901 gctgttgagg aattccaaat ctttggcttc atgcgtcgcg ccgagcatgt tggagtcggt
      961 ggagtaggct ttttcaaccg acattttgta gttgaagccg ttggcaatca gaaattcact
     1021 catttcgtgg cggccgccga gttcgtcgat aaattgttgg tcgagccaag gtttgtagat
     1081 tttcagcgcg ggattggtca acaggccgta gcggtagaaa cgctcgatgt cgttgccttt
     1141 gtaggtgctg ccgtcgcccc agatgtttac gtcgtcttct ttcattgcgg aaacgagcat
     1201 cgtgccggtt acggcgcggc ccagaggcgt ggtgttgaaa taggcgatgc cgccggtgga
     1261 aacgtggaac gcgccgcatt ggatggcggc gatgccttca tgtgccaact gcgcgcggca
     1321 gtcgattaag cgggcgtttt ccgcaccgta ttccatcgct tttttgggaa tggcgttgta
     1381 gtcgtcttca tcgggctgac cgaggttggc agtgtaggca taaggcagtg cgcctttgag
     1441 tttcatccac aacagcgcgg cggaggtatc aagtccgccg gagaaggcga tgccgacttt
     1501 ctgaccgacg gggaggtttt gtaaaatagt atggttttgg ctcataaggg tatccttcgg
     1561 acttggaaaa aggagttcgt cgtgtttcct tttcgattga aaccccgcca cttggacatc
     1621 tgtccttcgg ggcggtagaa tcagacttta tttgggaggg gcgtaatccc ttccaaatca
     1681 ggacggcaca tagggcggtg ctttatgtgt cgtcccgtgt gttgaaacat tatcttataa
     1741 atataaccca ttgtcagccg ttaaggctgt ctgaaaagaa gctttgcccg tttcgctgaa
     1801 aatcttttca gacggtcttt acgccatcct caacctgact ccctcccctg cgggggaggg
     1861 ttggggagag ggtgcagaca gcagccctta agcaaacgct gcctgaaaaa cgcaagctcg
     1921 accctctccc taaccctctc ccatgggaga gggaacaaat cacagcacca tcgacatttt
     1981 cagacggcat cggtaaagaa acccatctat gaacgcgttc aacctcacca tcatcggcga
     2041 cgaaatctta cacggcagcc gccaagacaa gcatttcgcc tttttcaaat ccctgctcga
     2101 atccaaaggg ctgaagctca atcaggtgca atatctgccc gacgaacccg atttgctggt
     2161 caggcaactg cgccgcagct tttcagacgg cataccgacg tttgttaccg gcggcatcgg
     2221 cgccacgccc gacgaccaca cgcgccaagc cgcagcggct gctttggatt tgcctgtcgt
     2281 ccgccatcct gaagccgcca agtttatcga aggcgtaacc cggaaacgcg gtgagccgct
     2341 cgattcgccc gaacacgccc aacgcctgaa gatggcggat tttccccaag gcgcggaatt
     2401 ggtgcctaat ccgtttaata atatcgccgg attttccatt cgcgagcatt atttcttccc
     2461 tggttttcct gtgatggcgc acccgatggc ggaatgggtt ttggatactt attacgtcgg
     2521 ccgcttcaac caaaccaaac gcggcagccg cagcgtgtat gtgtttgagc agcccgaatc
     2581 gcgcattacg ccgcttatgg agcatatcga gcaaacttat cccggtgtgc gttcgtacag
     2641 cctgcccagc gtcggttgga cgcattcaga cggcacgcag gtcaaaccgc acattgagtt
     2701 cggcatcaaa gccgaaggag aagccgtcaa ccttttggac gcggcgtggg cggaagtgtt
     2761 gcacagcttg gacggattgg gagcagagct gaaaaatcgg gtaaactgaa aaccgtttgc
     2821 ccaaatcgga cgatatttaa ggaaaaccat gcaataccga atcagaagag aaaacgaagc
     2881 acccgaagca aaaaatacgg gggaaacctt atgggagcgc gacatcatgc gcgaagtgct
     2941 gctgtccgcc tatcaggaca ggcgcaggga gcggatgtgg aaaaacatct ggcgtgccgt
     3001 cagcaccctg attctggttg ccctgattgc cggtattttc cgaaaagacg aagccgcatt
     3061 gcagttggcg ggcaacacgc cgcataccgc agtcgtcaac ctgtacggcg aaatcggcaa
     3121 cggtgtagaa gaccaggtca aaaaactcaa agacggtatg gaggccgcct acaaaaaccc
     3181 gcaggcaaaa gccatcgtca tccgcgccaa cagccccggc ggttcgcccg tcgtctccaa
     3241 caccgctttt gaagaaatac gccgtctgaa ggcgcagcac cccggcattc ccgtttatct
     3301 cgtggcggaa gatatgtgcg cgtccggctg ctactacatc gcggcggcgg cggacaaaat
     3361 ctatgccgac ccgtccagca tcgtcggcag catcggcgtt atcggcagca gtttcgacgc
     3421 gaccggcctg atggaaaaaa tcggcgtgaa acgcagggtt aaaattgcag gcagcaacaa
     3481 aggcatgggc gatccgtttt cgcccgaaac gcccgaacag tcgaaaatct gggaagaaat
     3541 gctgaccggc atacacggcg aattcatcaa agccgtcaaa accggacggg gcggccgcct
     3601 caaattccga caatatcccg acgtgttcag cggtcgcgtg tacacaggcg cggatgcgct
     3661 gaaagtcggg ctggtggacg gactcggcaa tatttacagc gtggcgcgag acgtggtcaa
     3721 agcccccgat gtggtggatt acactccgaa ggacgatttc ggcagaatct tgggcagacg
     3781 cttcggcgca gaactcaaag caagcgtgcg cgaagccctg caggccgtcc ggtagcgttg
     3841 tttacagtat ttctaaggga ataccaaaat aaaaatatac acaaaaacac tcacctcccc
     3901 agtatgctgc cactaagtca gccgcgtcac tatttaccgt gcacttaaag ccgtaaaagg
     3961 caggttgtcc ccgggaggtt ttgtgcatcc tgataccgat cggttgttgt tgctcaaagg
     4021 acagaaggcc gctgataaac gagattacct gtttgtcgct attgacgatt tttatactct
     4081 gccattttgc cagacaaaac cgcagacagt gctgccaagt ttctgaccga acatctggcc
     4141 gacccctgct tgtacctgat tgagtacgtt tactctgaca atgataggta atataaagag
     4201 ccgtccaaca tgctttcggt gcagcttgtt ataataatgg gattggttgg aggcttgccc
     4261 gatttgcttg tccgcagacc aacggtaagg cggagcgggt tatccgtacc ttgatggaga
     4321 tgtggcatga ggaacagtcg tttgacagtc cggaacatcg gcaaaaggag ttgtgctgct
     4381 ttattaattt aggggctgcc ctagatcact agggaaattc aaattaagtt agaattatcc
     4441 ctatgagaga aagtcgtcta agccggtata aacaaaataa actcattttg ttatcgtggc
     4501 tatgatgtat tggatgcggg cgaatttagc catttccgta tcaatcacag cacacatttt
     4561 gccgaacgac aaaaccatat taatggaa
//
LOCUS       DAWFJR010000120         4581 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWFJR010000120 DAWFJR010000000
VERSION     DAWFJR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.120"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(27..167)
                     /locus_tag="WGR04_001918"
     CDS             complement(27..167)
                     /locus_tag="WGR04_001918"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795414.1"
                     /translation="MPSENLSDGIFYQSSHIFYQGCKIYPKQQKPPCRHSRKSGNLEH
                     KI"
     gene            complement(185..1123)
                     /locus_tag="WGR04_001919"
     CDS             complement(185..1123)
                     /locus_tag="WGR04_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0795415.1"
                     /translation="MLELNGLCKRFGGKTVADDICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIIRPDGGEIRLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRNGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQNAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(1132..2007)
                     /locus_tag="WGR04_001920"
     CDS             complement(1132..2007)
                     /locus_tag="WGR04_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ0795416.1"
                     /translation="MPTVRFTESVSKHDLDALFEWAKASYGAESCWKTLYLNGLPLGN
                     LSSEWAERVKKDWEAGCSESSDGIFLNADGWSDMGSRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNILFSLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGRRSPHKAVDPDK
                     LDNTAAGGVSSGELPSEAVCRESSEEASLDKTLFPFIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAILPETFLPENQDGEVACFEKMDIGGLLDAMLSENMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2195..2271)
                     /locus_tag="WGR04_001921"
     tRNA            complement(2195..2271)
                     /locus_tag="WGR04_001921"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2235..2237),aa:Met,seq:cat)
     regulatory      2421..2520
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2611..4512
                     /gene="thiC"
                     /locus_tag="WGR04_001922"
     CDS             2611..4512
                     /gene="thiC"
                     /locus_tag="WGR04_001922"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ0795417.1"
                     /translation="MTTPKKTTKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPIYDTSGVYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIERAHDPQTAHLRFNQITRPRHAKSGSNVTQLHYARRGIITPE
                     MEFVAIRERMKLDELFRRPEYAKLLKQHAGQSFGANIPTHPDQITPEFVRREIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1108 a         1304 c         1202 g          967 t
ORIGIN      
        1 gaaacttgtc gggaaaaacg gtttctttag attttatgtt ctagattccc gcttttgcgg
       61 gaatgacggc agggtggttt ctgttgtttc ggataaattt tgcagccctg ataaaaaata
      121 tggctgcttt ggtaaaaaat gccgtctgaa aggttttcag acggcatttt gtttttaaga
      181 agcatcagcg gaagcggacg atttccctgt cttcgatatg gatgcgtacc gtatcgtttc
      241 ccgatatttc accggcgtgc ggcatatcga ggttcagcca caggatgccg tgttccggat
      301 ggaggacgga caggctgaac gattcgggca aacaggtacg ggataatacg cggcactcca
      361 tgccgtcttg gtcgaaacgc accgcatttt gcggaatatg gcggtcatcg tcggtattgg
      421 gcaaacccat cagtcgggcg acctgcacgc cggcaggcgt ttgaaccaag gtttcgggcg
      481 taccgcattg aaggattttc ccctcgtgca tgacggcgat ttcgtccgcc gccgtgcagg
      541 cctcttcggg cgaatgcgtt accaaaacgg cagggatgcc gccgtttcgg atacgttcgg
      601 cagtcatgcg gcgcagccgg tcgcgcaaat gcgtgtccaa actggaaaac gattcgtcca
      661 gcaacagcaa ggaagggcgg acaaccaaag cgcgcgccag tgccaaccgt tgcttctcgc
      721 ctccggaaag tttttcaggc ttgcggtgcg cctcattttc cagtccgact tcggcaagtg
      781 ccgccatggc aaggcgttcg gcttcggctt tcggcatttt ttgcattttc aaaccgaatg
      841 ccgcattttc cagcgcactc atatggggaa acagcgcgta atcttgaaac atcagcgaaa
      901 tacggcgttt ttcgggcggc atacgggtaa tgttttcccc gttcagccga atttcgccgc
      961 cgtccggacg gataatcccc gcaatcatat tcagcagtgt tgattttccg cagcccgacc
     1021 gccccaaaac ggcgagtatt ttgccgcgcc cgacagtcag gcagatatcg tcggcaaccg
     1081 ttttgccgcc gaagcgtttg cagagtccgt tcagttcaag catggcgcat cctataaacg
     1141 tatgccgtcc agccactcgg acagcggatg ggcggcatca atcagaccgt aacggcaaaa
     1201 cgcgtccagc gtaaccagtt gcgcgtcgtg catcatgttt tccgacaaca tggcatccaa
     1261 cagaccgccg atgtccatct tttcaaaaca cgcaacctcg ccgtcctgat tttcaggcag
     1321 gaaggtttcg ggcaggatgg catcgaatac atacaggatt tcattgtgca caccccggct
     1381 gacggggcgc aggctgtgca gccgggatac cggacggata aacggaaaca gcgttttatc
     1441 caaactcgct tcttcgctgc tttcgcggca cacggcttca gacggcaatt caccgctgga
     1501 aacaccgccg gcggcagtat tgtcgagttt gtcgggatcg actgctttgt gcggactgcg
     1561 cctgcctatc caaaaatgcc atctgccatc tgattcagtc agaccgttga gatggacggc
     1621 gcggctgagc agtccgaacg gacggaaagc ggcgcgttcg agtgagaaca agatattgcc
     1681 gccgccgtcg gtcaggtcga aacactcgtt gcgccatccg tgaagcagcc ccgccttgtt
     1741 ccatgtgcgg gcgaggtgct gcaagcggct gcccatatct gaccagccgt ccgcattcag
     1801 aaaaatgccg tctgaagact ccgagcagcc tgcctcccag tcttttttga cgcgctctgc
     1861 ccattccgat gacaggtttc ccaaaggcag gccgttcaga tacagtgttt tccagcaact
     1921 ttctgcaccg taacttgctt ttgcccactc gaacagggca tcaaggtcgt gtttgctgac
     1981 ggattcggta aaacggacgg tcggcatggt tggctttctt gaagacaatg cggaagattg
     2041 tagccaaact gcctgaattt gcaaccgcaa acagacggcg ggctgccgtt ccgatattgc
     2101 ggcaggaagg aaaatctggc aaaaaacgga ggcagcagga aacaggaaca ggcaaaacaa
     2161 aacccctcgg cattgaaccg aggggtttaa tatttggttg cgggagcagg attcgaacct
     2221 acgaccttcg ggttatgagc ccgacgagct accatgctgc tccatcccgc gtcagaagat
     2281 gaaactatac ggcagatttt ttatgttgtc aaacattact tccgcgaaaa atcataaaat
     2341 tttgccgtcc ggcggtttat gcctgatttg aaatattatt ttctttacaa aagttcatgt
     2401 tcgtgattta attttggtta acatcgaaac aggggtgctg cctgatgttc aggcggctga
     2461 gaaataccct ttacacccga tcgggataat acctgcgtgg ggagttttca cggattctgt
     2521 ctttcagacg gcattggttt tcaaatgccg tctgaaaacg caaaacgctc ctgtttcttt
     2581 attctaaacg agaaaacagg agcatttttt atgactacgc caaaaaaaac caccaaaact
     2641 tccggcaacg aagcgcgcga gcttgccgac ttgagcgaag acatcggcat ccgctttaaa
     2701 tatccgaact cggagcgcgt gtatctgcaa ggcagccgcg acgacatccg cgtgcctttg
     2761 cgtgaaatcc gtcaggacga cacttacacg gcgcaaggta cggaagccaa cccgccgatt
     2821 cccatctatg acaccagcgg cgtgtacggc gatccggcgg cacacatcga cctgaaacaa
     2881 ggcttgccgc acatccgcac cgcatggctg gatgaacgcg gcgataccga aatcctgccc
     2941 aagctttcta gcgaatacgg catcgaacgc gcgcacgacc cgcaaaccgc tcatctgcgt
     3001 ttcaatcaaa tcacccgtcc gcgccatgcg aaaagcggta gcaacgtaac ccagcttcac
     3061 tatgcgcgcc gcggcatcat tacgccggaa atggagtttg tcgccatacg cgaacgcatg
     3121 aagctggacg agcttttcag acggcctgaa tacgccaagc tcttgaaaca acacgcaggg
     3181 caaagtttcg gtgcgaacat cccgacccat cccgaccaaa tcacgcctga attcgtgcgc
     3241 cgagaaatcg ccgccggacg cgcgattatt cccgccaaca tcaaccaccc cgaactcgaa
     3301 ccgatgatta tcggccgcaa cttccgcgtc aaaatcaacg gcaacttggg caactccgcc
     3361 gtaacctcca gcctgaccga agaagtcgaa aaaatggtgt ggtcgctgcg ttggggtgcg
     3421 gacacgatta tggatttgtc caccggcgcg cacatccacg aaacacgcga atggattatc
     3481 cgcaacgcgc ccgtccccat cggcaccgtg ccgatttacc aagcgttgga aaaaaccggc
     3541 ggcatcgccg aagatttgac ttgggatttg ttccgcgaca ccctcatcga acaggcggag
     3601 caaggcgtgg actatttcac catacacgcg ggcgtgttgc tgcgttatgt gccgatgacc
     3661 gccaaccgcc tcaccggcat cgtctcgcgc ggcggttcga ttatggcgaa atggtgtttg
     3721 gcacatcatc gggaaaattt cctctacacg catttcgacg aaatctgcga aattatgaaa
     3781 gcgtatgacg tatcattcag cctcggcgac ggcctgcgcc ccggctgcat tgccgatgcc
     3841 aacgacgaat cccagttcgc cgaattgcac accttgggcg aattgaccga taaagcgtgg
     3901 aaacacgacg tacaagtcat gatcgaaggc cccgggcatg taccgctgca acgcgtcaaa
     3961 gaaaacatga ccgaagagct gcaacactgc tttgaagcac ctttttacac gctcggtccg
     4021 ctcgttaccg acatcgcacc cggctacgac cacatcacct cgggcatagg cgcggccaat
     4081 atcggctggt acggcaccgc catgctctgc tacgtgaccc cgaaagagca tctcggcctg
     4141 cccgacaaag aagacgtgcg caccggcatc attacctaca aactcgccgc tcacgccgcc
     4201 gacctcgcca aaggctggcc gggtgcgcaa ttacgcgaca acgccctgag caaggcgcgt
     4261 ttcgaattcc gctggcgcga ccaattccgc ttaagcctcg accccgaacg tgccgagagc
     4321 ttccacgacg aaaccctgcc tgccgaaggc gcgaaaatcg cccacttctg ctcgatgtgc
     4381 ggccccaaat tctgctcgat gaaaatcacg caggaagtgc gcgactacgc cgacaagcaa
     4441 aaagcccaac ggcagggaat ggaggaaaaa gcggtcgagt tcgtcaaaaa aggcgcgaag
     4501 atttacagtt gaaacgtcaa gcaaaaaatg ccgtctgaaa cccggaaaaa aggcttcaga
     4561 cggcattctt tcgcttgtga a
//
LOCUS       DAWFJR010000121         4580 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWFJR010000121 DAWFJR010000000
VERSION     DAWFJR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.121"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(40..153)
                     /gene="rrf"
                     /locus_tag="WGR04_001923"
     rRNA            complement(40..153)
                     /gene="rrf"
                     /locus_tag="WGR04_001923"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(250..3141)
                     /locus_tag="WGR04_001924"
     rRNA            complement(250..3141)
                     /locus_tag="WGR04_001924"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3549..3624)
                     /locus_tag="WGR04_001925"
     tRNA            complement(3549..3624)
                     /locus_tag="WGR04_001925"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3589..3591),aa:Ala,seq:tgc)
     gene            complement(3630..3706)
                     /locus_tag="WGR04_001926"
     tRNA            complement(3630..3706)
                     /locus_tag="WGR04_001926"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3670..3672),aa:Ile,seq:gat)
     gene            complement(3808..>4580)
                     /locus_tag="WGR04_001927"
     rRNA            complement(3808..>4580)
                     /locus_tag="WGR04_001927"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          988 a         1320 c          991 g         1281 t
ORIGIN      
        1 gcaaaaaccc ccgaataatc gggggttttc tgaatgggtg tttggcagtg acctactttc
       61 gcatggaaga accacactat catcggcgct gagtcgtttc acggtcctgt tcgggatggg
      121 aaggcgtggg accaactcgc tatggccgcc aaacttaaac tgttacaaat cggtaaagcc
      181 ttaatcaata tattcggtaa tgactgaatc agtcagtaag cttttatctc ttgaagttct
      241 tcaaatgata gagtcaagcc tcacgagcaa ttagtatggg ttagcttcac gcgttaccgc
      301 gcttccacac cccacctatc aacgtcctgg tctcgaacga ctctttagtg cggttaaacc
      361 gcaagggaag tctcatcttc aggcgagttt cgcgcttaga tgctttcagc gcttatctct
      421 tccgaactta gctacccggc tatgcaactg gcgttacaac cggtacacca gaggttcgtc
      481 cactccggtc ctctcgtact aggagcagcc cccgtcaaac ttccaacgcc cactgcagat
      541 agggaccaaa ctgtctcacg acgttttaaa cccagctcac gtaccacttt aaatggcgaa
      601 cagccatacc cttgggaccg actacagccc caggatgtga tgagccgaca tcgaggtgcc
      661 aaactccgcc gtcgatatga actcttgggc ggaatcagcc tgttatcccc ggagtacctt
      721 ttatccgttg agcgatggcc cttccataca gaaccaccgg atcactatgt cctgctttcg
      781 cacctgctcg acttgtcggt ctcgcagtta agctaccttt tgccattgca ctatcagtcc
      841 gatttccgac cggacctagg taaccttcga actcctccgt tacgctttgg gaggagaccg
      901 ccccagtcaa actgcctacc atgcacggtc cccgacccgg atgacgggtc tgggttagaa
      961 cctcaaagac accagggtgg tatttcaagg acggctccac agagactggc gtctctgctt
     1021 ctaagcctcc cacctatcct acacaagtga cttcaaagtc caatgcaaag ctacagtaaa
     1081 ggttcacggg gtctttccgt ctagcagcgg gtagattgca tcttcacaac cacttcaact
     1141 tcgctgagtc tcaggaggag acagtgtggc catcgttacg ccattcgtgc gggtcggaac
     1201 ttacccgaca aggaatttcg ctaccttagg accgttatag ttacggccgc cgtttactgg
     1261 ggcttcgatc cgatgctctc acatcttcaa ttaaccttcc agcaccgggc aggcgtcaca
     1321 ccctatacgt ccactttcgt gttagcagag tgctgtgttt ttaataaaca gtcgcagcca
     1381 cctattctct gcgaccctcc ggggcttacg gagcaagtcc ttaaccttag agggcatacc
     1441 ttctcccgaa gttacggtat caatttgccg agttccttct cctgagttct ctcaagcgcc
     1501 ttagaattct catcctgccc acctgtgtcg gtttgcggta cggttcgatt caaactgaag
     1561 cttagtggct tttcctggaa gcgtggtatc ggttgcttcg tgtccgtaga cactcgtcgt
     1621 cacttctcgg tgttaagaag acccggattt gcctaagtct tccacctacc ggcttaaaca
     1681 agctattcca acagcttgcc aacctaacct tctccgtccc cacatcgcat ttgaatcaag
     1741 tacaggaata ttaacctgtt tcccatcgac tacgcatttc tgcctcgcct taggggccga
     1801 ctcaccctac gccgatgaac gttgcgcagg aaaccttggg ctttcggcga gcgggctttt
     1861 cacccgcttt atcgctactc atgtcaacat tcgcacttct gatacctcca gcacacttta
     1921 caatgcacct tcatcggcct acagaacgct cccctaccat gccggtaaac cggcatccgc
     1981 agcttcggtt atagatttga gccccgttac atcttccgcg caggacgact cgaccagtga
     2041 gctattacgc tttctttaaa tgatggctgc ttctaagcca acatcctggc tgtctgggcc
     2101 ttcccacttc gtttaccact taatctatca ttggggacct tagctggcgg tctgggttgt
     2161 ttccctcttg acaacggacg ttagcacccg ctgtctgtct cccgaggaac cacttgatgg
     2221 tattctgagt ttgccatggg ttggtaagtt gcaataaccc cctagccata acagtgcttt
     2281 acccccatca gtgtcttgct cgaggcacta cctaaatagt tttcggggag aaccagctat
     2341 ctccgagttt gtttagcctt tcacccctat ccacagctca tccccgcatt ttgcaacatg
     2401 cgtgggttcg gtcctccagt acctgttacg gcaccttcaa cctggccatg gatagatcac
     2461 tcggtttcgg gtctacaccc agcaactcat cgccctatta agactcggtt tccctacgcc
     2521 tcccctattc ggttaagctc gctactgaat gtaagtcgtt gacccattat acaaaaggta
     2581 cgcagtcaca cctctagggc gctcccactg tttgtatgca tcaggtttca ggttctgttt
     2641 cactcccctc ccggggttct tttcgccttt ccctcacggt actggttcac tatcggtcga
     2701 tgatgagtat ttagccttgg aggatggtcc ccccatattc agacaggatt tcacgtgtcc
     2761 cgccctactt ttcgtacgct tagtaccgct gttgagattt cgaatacggg actgtcaccc
     2821 actatggtca agcttcccag cttgttcttc tatctcgaca gttattacgt acaggctcct
     2881 ccgcgttcgc tcgccactac ttgcggaatc tcggttgatt tcttttcctc cgggtactta
     2941 gatggttcag ttctccgggt tcgcttctct aagtctatgt attcaactta ggatactgca
     3001 cagaatgcag tgggtttccc cattcggaca tcgcggaatc attgctttat tgccagctcc
     3061 cccgcgcttt tcgcaggctt acacgtcctt cgtcgcctat catcgccaag gcatccacct
     3121 gatgcactta ttcacttgac tctatcattt caagaacttc tttgacttcg tttgcctacc
     3181 tgttgactaa gtaaaagcaa acttgaaatt cctactttga taaagcttac tgctttgttg
     3241 tgtcttaatc ctgccttttg tgtttcagga ttaagtcgat acaatcatca cccaaatact
     3301 gtgtttgttt tcttttctct cgcgagagat ttttatcctt tgcaaaaaat aaaaaatcaa
     3361 aacaaacgct ttgtctttgt ttgttgattt cggctttcca atttgttaaa gatcgatgcg
     3421 ttcgatatta ctatctactg tgcaagtcaa aacgagctga ttattatatc agcattttgt
     3481 tcttggtcaa gtgtgacgtc gccctgaatg gattctgttc cattcttccg ttttgatttg
     3541 tacagtattg gtggaggcaa acgggatcga accgatgacc ccctgcttgc aaagcaggtg
     3601 ctctaccaac tgagctatgc ccccgttctt ggtgggtctg ggaggacttg aacctccgac
     3661 cccacgctta tcaagcgtgt gctctaacca gctgagctac aaacccggat tctcttctta
     3721 agcgaatctt gccttcactc aagcttcttc cgcatctttt tcagtttacc gataagtgtg
     3781 aatgcctaaa gcctcttctt tctctagaaa ggaggtgatc cagccgcagg ttcccctacg
     3841 gctaccttgt tacgacttca ccccagtcat gaagcatacc gtggtaagcg ggctccttgc
     3901 ggttacccta cctacttctg gtatccccca ctcccatggt gtgacgggcg gtgtgtacaa
     3961 gacccgggaa cgtattcacc gcagtatgct gacctgcgat tactagcgat tccgacttca
     4021 tgcactcgag ttgcagagtg caatccggac tacgatcggt tttgtgagat tggctccgcc
     4081 tcgcggcttg gctaccctct gtaccgacca ttgtatgacg tgtgaagccc tggtcataag
     4141 ggccatgagg acttgacgtc atccccacct tcctccggct tgtcaccggc agtctcatta
     4201 gagtgcccaa ctgaatgatg gcaactaatg acaagggttg cgctcgttgc gggacttaac
     4261 ccaacatctc acgacacgag ctgacgacag ccatgcagca cctgtgttac ggctcccgaa
     4321 ggcactcctc cgtctccaga ggattccgta catgtcaaga ccaggtaagg ttcttcgcgt
     4381 tgcatcgaat taatccacat catccaccgc ttgtgcgggt ccccgtcaat tcctttgagt
     4441 tttaatcttg cgaccgtact ccccaggcgg tcaatttcac gcgttagcta cgctactaag
     4501 caatcaagtt gcccaacagc taattgacat cgtttagggc gtggactacc agggtatcta
     4561 atcctgtttg ctacccacgc
//
LOCUS       DAWFJR010000122         4417 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWFJR010000122 DAWFJR010000000
VERSION     DAWFJR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.122"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            298..750
                     /gene="dut"
                     /locus_tag="WGR04_001928"
     CDS             298..750
                     /gene="dut"
                     /locus_tag="WGR04_001928"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ0795418.1"
                     /translation="MNIEVEMKVLDERMADVVPVYATEGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVVVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            828..2015
                     /gene="dapC"
                     /locus_tag="WGR04_001929"
     CDS             828..2015
                     /gene="dapC"
                     /locus_tag="WGR04_001929"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ0795419.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTVDADNEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWNDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVVDVATCVKAAETIVS
                     LYR"
     gene            2178..2957
                     /gene="yaaA"
                     /locus_tag="WGR04_001930"
     CDS             2178..2957
                     /gene="yaaA"
                     /locus_tag="WGR04_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ0795420.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWHTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGQYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            3040..3130
                     /locus_tag="WGR04_001931"
     tRNA            3040..3130
                     /locus_tag="WGR04_001931"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3074..3076,aa:Ser,seq:tga)
     gene            3208..3297
                     /locus_tag="WGR04_001932"
     tRNA            3208..3297
                     /locus_tag="WGR04_001932"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3242..3244,aa:Ser,seq:cga)
     gene            complement(3491..>4417)
                     /locus_tag="WGR04_001933"
     CDS             complement(3491..>4417)
                     /locus_tag="WGR04_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0795421.1"
                     /translation="VIDWQPIEQYLNRQRTRYLRDHRGRPAYPLLSMFKAVLLGQWHS
                     LSDPELEHSLITRIDFNLFCRFDELSIPDYSTLCRYRNRLAQDDTLSELLELINCQLT
                     EKGLKVEKASAAVIDATIIQTAGSKQRQAIEVDEEGQVSGQTTPSKDSDARWTKKNGL
                     YKLGYKQHTRTDAEGYIEKLHITPANAHECKHLSPLLEGIAEGTTVYADKGYDSAENR
                     QHLKEHQLQDGIMRKACRNRPLSEAQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAY
                     FGLIKVSAQSHLKAMCLNLLKAPNRLSAPVAA"
BASE COUNT         1071 a         1062 c         1113 g         1171 t
ORIGIN      
        1 caaaaaaacc gaaaccgaac ggactagatt cccgcctgtg cgggaatgac gaatccatcc
       61 atacggaaac ctgcaccacg tcattcccac gaaagtggga atccagtttt ttgagtttca
      121 gtcattcccg ataaattgcc ttagtgtgtg cataatgttc gtcttcataa cgggaagggc
      181 gggcattata cactggcaac ggatttcaaa acaaaaccga tttgccgtgt ttcagcgtaa
      241 acacggcttg tgtataatct cccatctttg aaaccggccg tatgcaggag caagacgatg
      301 aatattgaag tagaaatgaa agtattggac gaacggatgg cggatgttgt ccctgtctat
      361 gcaacggagg gttctgcagg tttagatttg cgcgcctgtt tggatgagga agtcgttttg
      421 cagccgggtg aaacgtttct tgtgccgacg ggtttggcaa tttatttggc gaatcccgca
      481 tatgccgccg ttttgctgcc ccgttccggc ttggggcata aacacggcat tgttttgggc
      541 aatttggtcg gtttgattga ctccgattat caaggggaat tgaaggtgtc gttatggaac
      601 agaagcagcg aaccgtttac tgtcaaaccg tttgagcgta tcgcgcagat ggttgtcgtg
      661 ccaatcgtgc aggcgcgttt caaacgtgtc gaggaatttg tcggaagcag ccggggtgag
      721 ggcggcttcg gcagtacggg tttgcattga atataaaatg ccgtctgatg ggtgtcgtca
      781 ggttcggacg gcatatcttc ccaatatgcc ggtaatcgga gaacattatg aataccttac
      841 tcaaccaact caaaccctat ccctttgccc ggctgcgtga agcgatgcag ggcatttccg
      901 cccccgaagg tctggaagcc gtcccccttc acatcggcga accgaaacat ccaacaccga
      961 aagtcattac ggatgcgctg accgcctcat tgcacgagtt ggaaaaatat ccgctgactg
     1021 cgggtctgcc tgaactgcgt caggcgtgtg caaactggtt aaaacgccgt tatgacggct
     1081 taacagtgga tgcagataat gaaattctgc cggttttagg cagtagggag gcgttgtttt
     1141 cttttgttca aaccgtgttg aaccctgttt cagacggcat caaacccgca attgtcagcc
     1201 cgaatccctt ttatcagatt tacgaaggtg cgacactttt gggcggcggt gaaatccatt
     1261 ttgccaattg ccccgcgccg tctttcaacc ccgattggcg cagtatttcc gaagaggttt
     1321 ggaaacgcac caaactggtg ttcgtctgct cgcccaacaa ccccagcggc agcgtgctgg
     1381 atttggatgg atggaaagaa gtttttgatt tacaggataa atatggtttc attattgcct
     1441 cggatgaatg ctattccgaa atctatttcg acggcaacaa acctttgggc tgcctgcagg
     1501 ccgctgcaca gttggggcga agcaggcaaa aactgcttat gttcaccagt ttgtccaagc
     1561 gttccaacgt tccgggcctg cgttccggtt ttgtcgccgg cgatgccgaa ctgcttaaaa
     1621 actttctgct ttacagaacc tatcacggca gtgcaatgag cattcccgtg cagcgtgcca
     1681 gtattgccgc ctggaatgac gaacagcacg ttattgacaa ccgccgcctg tatcaggaaa
     1741 aatttgagcg cgttattccc attttgcaac aggtatttga cgttaaatta ccggatgcct
     1801 cgttttacat ctggttgaaa gtccccgatg gcgacgattt ggcatttgca cgcaatttat
     1861 ggcaaaaagc cgctatccaa gtattgcccg gacgtttttt ggcacgggat accgaacagg
     1921 gcaatcccgg ggaaggttat gtgcgtatcg ctttggttgt cgatgtcgca acttgtgtca
     1981 aagctgcgga aaccattgtt tccctatatc ggtaaagaat aaaaaatgcc gtctgaactt
     2041 ttgttcagac ggcatttttc aatattttac ggttgaattt gctgtaacgg tatttacttc
     2101 acttttaatc taatcggctt gcccgcaaac acgtttaaat ttaaaatccc cgtgtttgac
     2161 acaataccga gtggattatg ttttttgtcc tttcccctgc gaaaaacctt aacgaaaaag
     2221 accctgcccc tgtcagcgag tttacccaac ccgacctgct ggcagagtcc gacattctaa
     2281 tgcagcagtt gcgcgagctt gcgccgcaac agattgccga actgatgcac gtttccgaca
     2341 aaattgccct cttaaacgcg cagcgcaatg cagaatggca cacgccgttt acgccggaaa
     2401 acgccaaaca ggcggtcttt atgttcaacg gcgatgttta cgaaggtatg gatgcaaaca
     2461 cattggatat tggacagata cgctatctgc aaaaccatgt ccgcctgctg tccggtctgt
     2521 acggtcttct tcgcccgtta gacctgatac agccctatcg tttggaaatg gggacggcat
     2581 ttgccaattt gcgcggcaag aatttgtatg agttttgggg cgacatcatt accaaccttt
     2641 taaatgacac gcttgcccaa gcaggcagca atacgcttgt caaccttgcc tcacaggaat
     2701 atttcaagtc cgtcaacacg aaaaaacttc gggcgcggct gattaccccg atatttaaag
     2761 acgaaaaaaa cggtcaatat aaaatcatca gtttctatgc caagcgcgcg cgcggattaa
     2821 tggtgcgcta tgcggcagaa catcatatta ccgaccctga aatgctgaaa aattttaatt
     2881 acgaaggcta cgcattcaat gacgcggctt caaatgaaag cgaatgggtt tttatgcgtt
     2941 cggaacaaat aaagtgaaaa caataaatta agtattttcc gaaaaaagtg cttggcaaaa
     3001 tgtataaatt tcattattat tcctaatctt caagaagacg gaagcgtggc agagcggttt
     3061 aatgcaacgg tcttgaaaac cgtcgagggt tgatagccct ccgtgagttc gaatctcacc
     3121 gcttccgcca atccttgagg tcaaaaacca aataaaaatg aagtagcata aagcatcggc
     3181 atttttattt ggttttactt cattatcgga aacgtggcag agaggctgaa tgcagcggac
     3241 tcgaaatccg ctgagggtgc aaatcctccg tgggttcgaa tcccaccgtt tccgccagaa
     3301 aacataaccg ccctgattcg gggcggttct tttttgttca agattgagac ctttgcaaaa
     3361 ttcctttccc tcccgacagc cgaaacccaa acacaggttt tcggctgttt tcgccccaaa
     3421 tacctcctaa ttctacccaa ataccccctt aatccccgga tacccgataa tcaggcatcc
     3481 gggctgcttt ttaggcggca acaggcgcac ttaacctgtt gggcgctttc aacaggttca
     3541 aacacatcgc cttcaggtgg ctttgcgcac tcactttaat cagtccgaaa taggctgccc
     3601 gggcatagcg gaacttacgg tgcagcgtac cgaagctttg ttcaaccaca taacgggtct
     3661 tcgacaaata ccggttgcgt ttggtttgcg cttccgacag cggacggttg cggcaggctt
     3721 tgcgcataat gccgtcctgc aactgatgtt ctttcagatg ttgccggttt tccgcactgt
     3781 catagccttt gtcggcatag acggtcgtac cttcggctat cccttccagc aaaggcgaca
     3841 ggtgtttgca ctcatgggca ttggcggggg taatgtgcag tttctcgata tagccttctg
     3901 catcggtacg ggtatgctgt ttgtaaccga gtttgtagag gccgtttttc tttgtccaac
     3961 gggcatcgct gtctttactc ggtgtggttt ggccgctgac ttgtccttct tcgtcaactt
     4021 ctatggcctg acgctgtttg ctgccggcgg tctgaataat ggtggcgtca atgacggcgg
     4081 cggatgcttt ctctactttt aagccttttt cggtcagttg gcagttaatc agttccaaca
     4141 gttcggacag ggtgtcgtct tgcgccagcc ggttgcggta gcggcataag gtgctgtaat
     4201 cggggatgct cagttcgtca aaacggcaaa acaggttgaa gtcgatgcgg gtgatgaggc
     4261 tgtgttcgag ttcgggatcg gagaggctgt gccattgtcc gagcaggacg gctttgaaca
     4321 tggacaacag gggataggcg ggacggccgc ggtgatctct aaggtaacgg gttctttgac
     4381 gattcaggta ctgttcgatc ggctgccaat caatcac
//
LOCUS       DAWFJR010000123         4325 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWFJR010000123 DAWFJR010000000
VERSION     DAWFJR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.123"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            412..2298
                     /locus_tag="WGR04_001934"
     CDS             412..2298
                     /locus_tag="WGR04_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ0795422.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHAVLFADYGFGLPLTGVESERGGNLALHWFADCADTDAASWLARHSDGLPAGIST
                     PQSSVSEADYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDAEGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIQAQALPHTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLNPCKSGLGFEGIFNV
                     VIRTLLLKPVSNSVSDDLDSRLNLNQDKATKPQTRQDEATPYRFKVNPLYQGVYGVGS
                     GIVIDSDPAAEYRECGWKARFLNELRPDFGIFETLRAENGRCTLLDRHLCRLKTSAQA
                     LNLPLPDGCENQIKQYIADLPDGAFRVKALLASDGISLSRAVLNRLTDKQRVIISPTI
                     LPAQNYLRRFKTTCRTVFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKHRGQW
                     LTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETHITQKTLQEAEEIRLSNALRGVFA
                     AALA"
     gene            complement(2357..3874)
                     /locus_tag="WGR04_001935"
     CDS             complement(2357..3874)
                     /locus_tag="WGR04_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0795423.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAEKLDLPAPVLENVGRFRKKAEGLTGWRFSGGISPAVNQNANNAAPQYCRQDGGS
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGIAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
BASE COUNT         1068 a         1391 c          955 g          911 t
ORIGIN      
        1 ctgcagatgc ccgacggtct ttatagcgga ttaacaaaaa tcaggacaag acaacgaagc
       61 cgaagacagt acagatagta cggaaccgat tcacttggtg cttcagcacc tgagagaatc
      121 gttctctttg agctaaagcg aggcaacgct gtactggttt ttgttaatcc actatacttg
      181 gagctggtct tgcttttcgc ctaattctac gttttcaaac ggttgcagct ggtggtctgt
      241 cataaaggtc tccttattgt atttcaggtt ggaaatcgga atttgttttc acaattttac
      301 accttcgccc ccactttctc tacataaaat tacattttgc cgatatttgc cgaatcgtct
      361 gaaaatatgt gtaaaaagag cgtataatca aaacatttgc cccgaattgc catgccttat
      421 ttcgccctgt ttgacgatgc cgtaagcggc cgcgcaaaac gctatcaaaa tcatgtggaa
      481 agccgttttt tccgtcccga agaactcgat gctttggacg gcgcgctgca atcgggctgg
      541 caaaaagggc tgcatgccgt gttgtttgca gactacggat tcggtttgcc gctgacgggg
      601 gtcgagtccg aacgcggcgg caaccttgcc ctgcactggt ttgccgactg cgccgacacc
      661 gatgccgcaa gctggcttgc ccgacactca gacggcctcc ccgccggcat ttccacgccg
      721 caatcctccg tatccgaagc cgattacctc gaccatatcc gccaaatcca cgaagccatc
      781 cgacgcggcg acacctatca aatcaactac accacccgcc tgcacctgca agcctacggc
      841 aatcccgtca gcctctaccg ccgcctgcgc cagcccgtcc cctatgccgt tttgtcccac
      901 ctgcccgatg cggaagggca atccgcgtgg acgctttgtt tctcgcccga actcttcctc
      961 aacatcgctt cagacggcac catcagcacc gaaccgatga aaggcaccgc gccgattttg
     1021 ggcgacggac aagacgaacg ccgcgccgcc gaattgcaag ccgacccgaa aaaccgcgcc
     1081 gaaaacgtga tgattgtcga tttgctgcgc aacgacctcg gcaaaatcgc ccaaaccggc
     1141 aaagtatgcg tgcccgagcc gtttaaagta tcacgtttcg gcagcgtttg gcagatgacc
     1201 agcaccatcc aagcccaagc cctgccgcac acctcgttcg ccgacatcct ccgcgccgcc
     1261 ttcccctgcg gcagcatcac cggtgcgccc aaaaaaatga gtatgcagat tatcgaatcg
     1321 ctcgaaaccg aagcgcgcgg cctgtacacc ggcagcatcg gctacctgaa tccatgtaaa
     1381 agtgggctgg ggtttgaagg catattcaac gtcgttatcc gcaccttgtt gctcaaaccc
     1441 gtttctaact cagtttcaga cgacctagat agtcgattaa atttgaatca ggacaaggcg
     1501 acgaaaccgc agacacggca agacgaggca acgccgtacc ggtttaaagt taatccactc
     1561 tatcaagggg tgtacggtgt cggttccggc atcgtcatcg acagcgaccc cgccgccgaa
     1621 tatcgcgaat gcggctggaa agcccgtttc ctcaacgaat tgcgccccga cttcggcatt
     1681 tttgaaaccc tgcgcgcgga aaacggacgc tgcaccctgc tcgaccgcca cctatgccgt
     1741 ctgaaaacct ccgcccaagc cctcaacctg cccctgcccg acggctgcga aaatcaaatc
     1801 aaacaataca ttgccgactt gcccgatggc gcgttccgcg tcaaagccct gctcgcttca
     1861 gacggcatca gcctgtcccg cgccgtttta aaccgtctga ccgacaaaca acgcgtcatc
     1921 atttcgccga ccatcctgcc cgcacaaaac tacctgcgcc gcttcaaaac cacctgccgc
     1981 accgtcttcg accaagcgtg gcaaaccgcc gaaacacaag gcgcgttcga cagcctgttt
     2041 ttcaattcag acggcatcct gctcgaaggc ggcagaagca acgtgttcgt caaacatcgc
     2101 ggacaatggc tcacgccctc cttagattta gacattttaa acggcataat gcgccaagcc
     2161 gtattggacg aaccgcaaaa atatttgcaa acaaatcaag taatcgaaac acacatcaca
     2221 caaaaaacac tgcaagaagc cgaagaaatc cgcctctcca acgccttgcg cggcgtattt
     2281 gccgccgccc ttgcctgaac gcgcaaaaat gccgtccgaa cctgtttcca aagttcggac
     2341 ggcattatcc caccattcaa aaccgccaat ccgccgacac aaacacctcg ctgttgcggc
     2401 gtttcgcata cggcacatta ctttccgtcc tgccgaaacg ataattcaac gccggcacga
     2461 tacctttgta cgacaacttg tcgtggctca aagccagcga gacattccat tcgcggttgc
     2521 gttgcgcctc tgtcgagaaa gccgcaatgc ccttatagtt gcggcgggca taagacgcag
     2581 aaacccgact gttcaaaccg cccaactgcc gccactcctg cgcccagccg gcataaacac
     2641 cgttgcgccg gtaggcggca ttattgaccg cgccgcccac cgtttcgcgt ttcggcacaa
     2701 accgcacaaa ctgccagccg ccgaacacag ttgccgattc gcccaaacgt tttgccgacg
     2761 aaacataaaa cccgtcctgc ctgccgttat tgtattccgc cctatcctgt tcgcggtagc
     2821 gttggcggta atgttccagc gcgaccgaaa attgccatcc cgggtttggg cggtaagtat
     2881 gggacagctg cacgccgact ccgtgcgcca gcatatacgg cggcaggcgg cggttgttta
     2941 cccgttttgt tttcgcatca aagccgtcgc tgcccgacaa ctgcacctga taaaacggca
     3001 aaatccccgc cgtctgccgt gcatttttat actgccatcc caaatacgcc ctgccgaacc
     3061 cgtcatcata agccgatttt ttactgaaat aatagcttgt gccgccgata ttggaacgga
     3121 acaacaaata atgattgtcc gccaacgccg tcagcttttc cgcctcgatt tcataattca
     3181 agcctgccgc ccgctccgcc cggctgacac tgcatatctg actgcctccg tcttgccggc
     3241 aatattgcgg cgcggcatta ttggcgtttt gattgaccgc cgggctgatg ccgcccgaaa
     3301 aacgccagcc cgtcagcccc tccgcttttt tccgaaaacg ccccacattt tccaaaaccg
     3361 gcgccggcaa atccaatttt tccgcctcgg caaaatgcct ttctgccgac ttcagccgga
     3421 aatcgtcaaa ctccgccgcc gccaaatcca gcaaaatccg ctcgtctgcc gcattttccc
     3481 cgtgcagttc ccgataccgc gccaccgcct ccgccggcct tcccgccaat ttcgccagca
     3541 aagcccgcgc cctgccgtac aaaaccgcgt cataatccgg cagcttggca tacaaatccg
     3601 ccaacgaagc gattaaatcc gcctgattgc cgttgagcgc gtcgcgcaaa ctatgttcca
     3661 acattttcgg atgtgccaac aaaaaatccc cgtcaaccac gcgcggggca tcattttcaa
     3721 ctttccaatc tgattccgcc cacttatccg acaccgaccg ctgcacctgc aacaatgcct
     3781 tgtcatccaa aatcgcgggc gcatccgccc cataggcggc agaaacacct gccgcacacc
     3841 aaacaaccaa aaagccgtat ctgaaataca acataccctg tcatttacct ttccggcaaa
     3901 cacgccgccg aagcacgtca aaccatccga aaaacaggca gaaacccgtg aaaaccggct
     3961 ttgccgtctg aaagcgggca ggcaaaaaac cgccgcccga ttttcaaagg gcggatttca
     4021 catttatagt ggattaacaa aaatcaggac aagacgacga agccgcagac agtacagata
     4081 gtacggaacc gattcactcg gtgcttcagc accttagaga atcgttctct ttgagctaag
     4141 gcgaggcaac gccgtaccgg tttttgttaa tccactatac cgatttttgc accgacccac
     4201 aaaaccagtt gacatcatat aacatttaaa ctgataatca aaactattat taacacatca
     4261 tcaagattga gtatgcgatg aaatacaaag ccctctcctt actgccgctt gctgccgccc
     4321 ttgcc
//
LOCUS       DAWFJR010000124         4211 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWFJR010000124 DAWFJR010000000
VERSION     DAWFJR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.124"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..1066
                     /locus_tag="WGR04_001936"
     CDS             <1..1066
                     /locus_tag="WGR04_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2,9-polysialyltransferase"
                     /protein_id="HEZ0795424.1"
                     /translation="KKKNITTHLIDEGTGTYAPLLESFSYHPTKLERYLIGNNLNIKG
                     YIDHFDILHVPFPEYAKKIFNAKKYNRFFAHAGGISINNNIANLQKKYQISKNDYIFV
                     SQRYPISDDLYYKSIVEILNSISLQIKGKIFIKLHPKEMGNNYVMSLFLNMVEINPRL
                     VVINEPPFLIEPLIYLTNPKGIIGLASSSLIYTPLLSPSTQCLSIGELIINLIQKYSM
                     VENTEMIQEHLEIIKKFNFINILNDLNGVISNPLFKTEETFETLLKSAEFAYKSKNYF
                     QAIFYWQLASKNNITLLGHKALWYYNALYNVKQIYKMEYSDIFYIDNISVDFHSKDKL
                     TWEKIKHYYYSADNRIGRDR"
     gene            complement(1562..2515)
                     /locus_tag="WGR04_001937"
     CDS             complement(1562..2515)
                     /locus_tag="WGR04_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795425.1"
                     /translation="MHQYYDAVILSVGNGLLKRFFKQNAQLNIASRPLIITLFPGVVF
                     GDQASILSRMGADIVLYNNKHDFRIAEEYKKQYKLSCQNILYGYPIFRHASKGRHGEK
                     IYFIDQVKIPFKKEERIYTLKKLISLAEKYPEKEFTILLRVADKDITVHQDKHSYIEL
                     AKQFQLPSNLTIERKSTAQAFQEMGYCLSYSSTMLFEAECKGIPVGVVADLGFSKSYA
                     NQHFLGSGVLVYFDQIDFTSPKIADPDWLDCYATKKVITTDEFNKLLKQVVPLQHDYQ
                     EYLSAVNSIESTKTIFLRKFKKLIRDPKKFFYDSKWLRKVI"
     gene            2898..3917
                     /gene="galE"
                     /locus_tag="WGR04_001938"
     CDS             2898..3917
                     /gene="galE"
                     /locus_tag="WGR04_001938"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ0795426.1"
                     /translation="MKKILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPPGDTTSPYGASKSMVERI
                     LTDIQKADPRWSMILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAV
                     FGDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRA
                     FEAASGLTIPYEVKPRRAGDLACFYADPSYTKAQIGWQTQRDLTQMMEDSWRWVSNHP
                     NGYDD"
     gene            4033..>4211
                     /locus_tag="WGR04_001939"
     CDS             4033..>4211
                     /locus_tag="WGR04_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEZ0795427.1"
                     /translation="MRKILVTGGAGFIGSAVVRHIIQNTQDSVVNLDKLTYAGNLESL
                     TDIADNPRYAFEQVD"
BASE COUNT         1350 a          803 c          741 g         1317 t
ORIGIN      
        1 aaaaaaaaag aatattacga ctcatttaat tgatgaaggg actggaacat atgctccttt
       61 attagaatca ttttcatatc atccaacaaa attagaacgt tatttgattg gaaataatct
      121 taatattaaa ggatatatag atcattttga catattgcat gtcccctttc ctgaatatgc
      181 taaaaaaata tttaatgcaa aaaaatataa ccggtttttt gcgcatgctg gaggaataag
      241 cattaataat aacattgcaa acttacagaa aaaatatcaa atatctaaaa atgactatat
      301 ttttgttagt caacgctacc ccatttcaga tgatttgtat tataagagta tagtagaaat
      361 cttaaacagc ataagtttac aaattaaagg aaagatattt attaaactac acccaaaaga
      421 gatgggcaac aactatgtaa tgtctttatt tctaaatatg gtagaaataa accctcggct
      481 ggtagttatt aatgaacctc cttttctaat tgagccccta atatacttaa caaatcctaa
      541 aggaattata ggcctggcct ctagttcttt aatttataca ccattactct caccctcaac
      601 ccaatgtctt tctattggag agttaattat taacttaatt caaaaatatt caatggtgga
      661 aaacactgaa atgatccaag aacacttaga gattattaag aaatttaatt ttattaatat
      721 actaaatgat ttaaatgggg taataagtaa ccctctcttt aaaacagaag aaacatttga
      781 aacacttctt aaatctgcag aattcgcata taaatctaaa aactactttc aggctatttt
      841 ttactggcaa cttgccagca aaaacaatat taccttatta gggcataaag cattatggta
      901 ctacaatgca ctttataatg taaaacaaat ttataagatg gaatattcag atatttttta
      961 tatcgataat atctccgtag actttcatag taaagataaa ttgacatggg aaaaaattaa
     1021 acattattac tattccgccg acaatagaat tggtagagat agataaaaat actatgactt
     1081 ctgatctcca tacgtatgct tgttttcaaa aagcagcatt aagatagata agtgaagctt
     1141 gatgagagcc atttttgtgg tcatcgccaa ggtacacgcg gagagtaatc gacaacagag
     1201 ctgctgtatt ctggctttta aagcccagta gtaaagttta taaggctgca tttctcaatg
     1261 taaaacgcga tattttcacc cattattagg gataagtagc cagtggttta cactggctac
     1321 tttcatagtt atgaagtact gggtctgagt gaattcaatt atttccgtat aaaacacagt
     1381 agacatttta ctaatcggca aaattatatt agaagaattg agaactactg aagtcaggta
     1441 agataccatt ttgcacaaat ttaacggcat ccctaaaaag cattttgggt ggtatttaaa
     1501 ggagtacaaa atggttttaa ctacagtatg cttagaaatc tatagcctta cgcgctttat
     1561 attaaatcac ctttctcaac catttagaat cataaaaaaa ctttttagga tccctaatta
     1621 actttttaaa ttttcttaaa aaaatagttt tagttgattc tatactattc accgcagata
     1681 aatattcttg gtaatcatgt tgcaatggca caacctgctt taatagctta ttaaactcat
     1741 cagttgtaat caccttttta gtagcatagc aatcaagcca atccggatct gctatttttg
     1801 gggaagtgaa atctatttga tcaaaataaa ctaaaacccc actacctaaa aaatgctgat
     1861 ttgcatagga tttagaaaag cctaagtctg caacaacacc aacagggata cccttacatt
     1921 cagcttcaaa aagcatagta gatgaataag ataaacaata ccccatttct tggaaggctt
     1981 gcgcggtact ttttcgctct attgtcaaat tactcggcaa ctgaaactgc tttgccagct
     2041 ctatatacga atgtttatcc tgatgcacag taatatcttt atctgcaacc cttagcaaaa
     2101 tagtaaattc tttctcaggg tatttttcag ccaaggaaat caattttttt aatgtataaa
     2161 ttctttcttc ttttttaaat gggattttaa cttggtcaat aaagtaaatt ttctctccat
     2221 gacgaccttt tgatgcgtgg cgaaaaattg gataaccata aagtatattt tgacaactta
     2281 atttatattg tttcttatat tcctctgcaa ttctaaaatc atgcttatta ttatataaaa
     2341 caatatcagc ccccatacga gatagaatac ttgcctgatc accgaatact acacctggaa
     2401 acaaggtaat aatcaatggc cttgaagcaa tatttaattg cgcattctgc ttaaagaacc
     2461 tttttaacaa cccatttcca accgataaaa ttactgcgtc ataatattga tgcatattct
     2521 taataaagta atcatcaata tgaaaaaaaa aattgcttgg tctttatcta tccctgattc
     2581 taataactgt cgatttgaaa gaatgttttc tcgacttttg tggatataaa tatcaatttg
     2641 agcatctttt atctcttttg caacagcata gcccgagtta aggaacgagt cataactggc
     2701 aattagtaat acttttttca ctattttccc ttaattctac accatatgaa taaaattata
     2761 gtattttacc ttagatatca aatatactta accaaaaaaa tctgaagaaa tctactgcta
     2821 cttcagggat aattatttca ttaagatttt acaaccatta ttatcaatct atcaaagtat
     2881 ttttagtatt ttattctatg aaaaaaattc tcgttaccgg cggcaccggt tttatcggct
     2941 cgcataccgt tgtttctttg ctgaaaagcg gccatcaagt cgtgattttg gataacctat
     3001 gcaattccag catcaatatc ctgccacgct tgaaaacgat taccggccaa gagattccgt
     3061 tttatcaagg cgatatccgc gatcgtgaga ttttgcgccg tatttttgcg gaaaaccgca
     3121 ttgattcggt gattcatttt gccggcttga aagcggtggg tgaaagtgtg gccgagccga
     3181 tgaaatatta tgataataat gtttccggca gcttggtgtt ggcggaagaa atggcgcgtg
     3241 cgggcgtgtt tagcattgtg ttcagttctt cggcgacggt ttatggcgat ccgggcaaag
     3301 tgccttatac cgaggatatg ccaccgggcg acaccaccag cccttacggc gcatcgaaat
     3361 cgatggttga gcgcattctc actgacattc aaaaagccga tccgcgctgg agcatgattt
     3421 tgttgcgtta tttcaatccg attggcgcgc atgaaagcgg tttgattggc gagcagccaa
     3481 acggtatccc aaataatttg ttgccttata tctgccaagt ggcggcaggc aaactgccgc
     3541 aattggcggt atttggcgat gactacccta cccccgacgg cacggggatg cgtgactata
     3601 ttcatgtgat ggatttggca gaaggccatg tcgcggctat gcaggcaaaa agtaatgtag
     3661 caggcacgca tttgctgaac ttaggctccg gccgcgcttc ttcggtgttg gaaatcatcc
     3721 gcgcatttga agcagcttcg ggtttgacga ttccgtatga agtcaaaccg cgccgtgccg
     3781 gtgatttggc gtgcttctat gccgaccctt cctatacaaa ggcgcaaatc ggctggcaaa
     3841 cccagcgtga tttaacccaa atgatggaag actcatggcg ctgggtgagt aatcatccga
     3901 atggctacga cgattaagtt gacctgatac aggccgtctg aaagagatgt tttcagacgg
     3961 cctctttatc tgaaaaacac acattctgtc tgctataatc tgtttatatt ttttggctat
     4021 cctctgaaat ttatgagaaa aatccttgtt accggcggcg cgggctttat cggctctgcc
     4081 gttgtccgcc atatcattca aaacacccaa gattccgtcg tcaacctcga caaactgacc
     4141 tacgcgggca atctcgaatc gctgaccgac attgccgata atccccgcta tgctttcgag
     4201 caagtggaca t
//
LOCUS       DAWFJR010000125         4208 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWFJR010000125 DAWFJR010000000
VERSION     DAWFJR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.125"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..890
                     /locus_tag="WGR04_001940"
     CDS             <1..890
                     /locus_tag="WGR04_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0795428.1"
                     /translation="TAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKA
                     NLPKDAQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNPALKNLSP
                     AQRRDFADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQH
                     ILIKADSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVM
                     VPAFEEAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAE
                     QATVNLLRDLHSGAYVDIR"
     gene            complement(2193..4073)
                     /locus_tag="WGR04_001941"
     CDS             complement(2193..4073)
                     /locus_tag="WGR04_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEZ0795429.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTPHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKHGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKISAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLALPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLDNGTKRVGIHIAAPS
                     LAVKPGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAHRPAVSIYFDVDSE
                     FNTGEPTCKIEAVNISENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDEEGKVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
BASE COUNT          963 a         1077 c         1200 g          968 t
ORIGIN      
        1 ccacggcgca aaaagaagcc gcacccgcac aacagcaggg cggtatccgc ttttcagacg
       61 gcattgccgc cgttgccgac aacgaagtca tcacgcgccg ccggcttgcc gaagccgttg
      121 ccgaagccaa agccaacctg cccaaagacg cgcagataag cgaatccgag ctgtcccgac
      181 aggtgctgat gcagcttgtc aaccaatccc tgattgtaca ggcgggcaaa cgccgcaaca
      241 ttcaagcaag cgaagcggaa atcgatgccg tcgtcgcaaa aaatcccgcc ctcaaaaacc
      301 tcagccccgc ccaacgccgc gattttgccg acaacatcat tgccgaaaaa gtccgccagc
      361 aggcagtgat gcagaacagc cgagtgagcg aagctgaaat cgatgccttc ctcgagcagg
      421 cgcaaaaaca aggcatcacc ctgcccgaag gcgcaccgtt gcgccaatac cgcgcccaac
      481 acatcctgat taaagccgac agcgaaaacg ctgccgtcgg cgcggaaagc accatccgca
      541 aaatctacgg agaggcccgc agcggcacag acttttccag cctggcgcgc caatattcgc
      601 aagacgcgag cgcgggcaac ggcggagatt tgggctggtt tgccgacggc gtgatggttc
      661 ccgcctttga agaagccgtc cacgcgctca aacccggaca ggtcggcgcg cccgtccgca
      721 cccaattcgg ctggcatatc atcaaattga acgaagtgcg cgatgccggc acacctcagg
      781 aacgtatccg caattccgtg cggcaataca tcttccaaca aaaagccgaa caggcaaccg
      841 tcaacctgtt gcgtgacctg cattccggcg cgtatgtcga catccgctaa ggcggtttga
      901 agcaaaaagc cataccgatc ggcaaaaatc cgggcggtat ggctttttgg atttcgagtt
      961 acttttacac cgtcattcat cattcccgcg aaagcgggaa tctagaaacg aaaagtaaca
     1021 ggaatttatc gggaatggct ggagtttaaa ggactggatt cccgccgtcg cgggaatgac
     1081 gggattttgg gttgtggtaa tttatcggaa aaacaaaaaa acctatgccg tcattcccga
     1141 gcaggcggga atccggttat ttaaaactgc agaaatttat ccgaagcaac aacaatcttt
     1201 ccatcgtcat tcccgcgtag gcgggaatct agcaacgaaa agtaacagga atttatcggg
     1261 aaaacagaaa cctctatgcc gtcattcccg cgaaagcggg aatctaggac gtagaatcta
     1321 aagaaaccgt tttatccgat aagtttctgt gccgaagggt ctggatttcc gctttcgcgg
     1381 gaatgacggc gcataagttc ccgtgcggac agacctagat tcccacctgt gtgggaatga
     1441 cgattcagaa gttgcccgaa actcaaaaaa aacgaaaccg aacgaaccgg attcccgctt
     1501 tcgcgggaat gacggcgcat aagttcccgt gcggacagac ctagattccc acctgtgtgg
     1561 gaatgacgat gcagaagttg cccgaaacca aaaaactgaa gccgaacggt ctggattccc
     1621 gctttcgcgg gaatgacggc gcataagttc ccgtgcggac agacctagat tcccacctgc
     1681 gtgggaatga cgattcagaa gttgcccgaa accaaaaaac tgaagccgaa cggtctggat
     1741 tcccgctttc gcgggaatga cgggattttg ggttgtggta atttatcggg aaaacggaaa
     1801 cccctatgcc gtcattcccg cgcaggcggg aatctaggac gtagaatcta aagaaaccgt
     1861 tttatccgat aagtttctgt accgaagaat ctggatttcc gctttcgcgg gaatgacggc
     1921 gcataagttc ccgtgcggac agacctagat tcccacctgc gtgggaatga cgattcagaa
     1981 gttgcctgaa acctaaaaaa ctgaaaccga acgagccgga tttccgcttt cgcgggaatg
     2041 acgggatttt agattgcggg tatttatcgg gaacggcggc ttggaagttc attgaaacgg
     2101 aaaaacaacg gaaacccaaa aaaccggatt cccgactgtg ggaatgatga gattcaggtt
     2161 tctgtttttg ccggagtttg ccgtatcggg cttcagacgg cattgcctgc cgttgtaccc
     2221 gcgggtgcga ctgccttgat gtagttgagc gagacaaact gcttctcggc atccaattcg
     2281 gtgattttga acaatgcctg tgatttgggc agtgcgtcaa acggaatacc ggtcgcgcgc
     2341 gtgaccagcg gcaggccttc gatgcggacg aggtcttctt tgaggatggt cgcggtcagc
     2401 tcgcttgtac cttgctgttg caggtacaca aggctccagt aggcttccat ctgccgttgg
     2461 aaatcggcgt aggcggtata ggcggcatca aagtcgcgca gtgcggcgaa aagctcggca
     2521 tcgctgtttt gatacagcgg ctcggcagtg tcgtctatca ggctgatcag ctgcttttgg
     2581 ttgatgtagt cggcggcgcg gcgcagcggc gaggtaaacc agccgtaatg ctgcacgccc
     2641 atgccgatat gcggctcgga tttggtgctc atgcgtactt ttccggtggg ttggacgcgg
     2701 aagaggccgg gcaggtcgtt gtcatggagc atttgtgccc aagtgctgtt ggcaagaatc
     2761 atcatctcgc tgaccagcgt atcgatgggt gagccgcgtt cgcggcggac gacggatacc
     2821 ttgccttcct catccaattc gatgctgtaa tcgtattgcg gcgcgcggtc gggttcgtat
     2881 ttgccgcgcg ctttttgcag ggcggtggcg aattgataga accaaatcag gtcttgatgg
     2941 tgggcgaaca tcatttcgcc cgcttcgtcc aagcccgctt cggcgttgaa atgcggttcg
     3001 atggcttgga tacgcaggtt ttcggagatg ttgaccgctt cgattttgca ggtcggttcg
     3061 ccggtgttga actcgctgtc cacatcgaaa taaatgctga cggcagggcg gtgtgcgcct
     3121 gcatcaaggc tgaacgcggc aatccagttt tcgggcagca tcgtgatttt gccgccgggg
     3181 aaataaaccg tgctcaagcg ttccatgatg tttttttcca ttttgtcgcc cggtttaacg
     3241 gcaagtgacg gcgcggcgat gtggatgccg acacgcttcg tgccgttgtc caagtcggtc
     3301 aggcttaaag cgtcgtccac ttcggtggtc gattcgtcgt caatggaaaa ggcggtaacg
     3361 tcggccttgg gcaggtcggg catttcggga agggcaaggt cggggaagcc tgtgccttta
     3421 ggaaagtatt tgatttcaaa cccgtcttgc aggtattgcg gaatggacgt aatgccaccc
     3481 gtttttttcg ccaattcgta ggcggagatt tttagtgcgt cggcggcttt ggtaaaggct
     3541 ttgtaggtca gcgactgctt gtcgggcgcg tgcaggatgg ttttcaaatc cgccgcgatt
     3601 tcagacggca tctcgccgtg tttcaaggct tctgcccaag cgtcgatttg tgcgtcctgc
     3661 tgttttttgc gttcgatggc ggcaagcgct tgttttaagg tttcttcggg cgcggctttg
     3721 aacacgcctt tggctttttt gtagaaatac atcggcgcgg cgtaaagtgc aatcaaagtc
     3781 gccgccagct cggttttggt cggcgcgtgt ccgtaatatt cttcggcgat ggcttcggcg
     3841 gtaaattctt cttcgccgca tacctcccac aataaatcgg tgtcgatgtc cgccgcctgc
     3901 gcctgcgcgt tctccaaaaa cgccgccata tcgccgtcga actcggcaaa gacgttgttc
     3961 gccttcactt tggtgcgttt gccgtgtggg gtatcgactt ggtaggtggc atcgtttttt
     4021 tggatgatgg cggcgatttt gaattggccg gactcttcgt aaaaaatatt catttttcgg
     4081 atttttctgt ggaaactcaa gcgggcgatt ttagcagatt accgaaaatg ccgtctgaaa
     4141 aaaggttggg ggagggttgg cgcgtctttg cggtgcttgc gttatagtgg attaacaaaa
     4201 accagtac
//
LOCUS       DAWFJR010000126         4181 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWFJR010000126 DAWFJR010000000
VERSION     DAWFJR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.126"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..104)
                     /locus_tag="WGR04_001942"
     CDS             complement(<1..104)
                     /locus_tag="WGR04_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0795430.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPI"
     gene            complement(232..1482)
                     /locus_tag="WGR04_001943"
     CDS             complement(232..1482)
                     /locus_tag="WGR04_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ0795431.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     ETQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLIAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYPVYGA"
     gene            complement(1548..1760)
                     /locus_tag="WGR04_001944"
     CDS             complement(1548..1760)
                     /locus_tag="WGR04_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795432.1"
                     /translation="MADKDVVGAGYLIDFAQTAENLPFNVLLLISLVLNKGDETLKTS
                     MLNKLPMLNKLPDNAKENLRIMGYLP"
     gene            complement(1981..3756)
                     /gene="ggt"
                     /locus_tag="WGR04_001945"
     CDS             complement(1981..3756)
                     /gene="ggt"
                     /locus_tag="WGR04_001945"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ0795433.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKVLHAGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGNYELETGTTAVQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3924..4181)
                     /locus_tag="WGR04_001946"
                     /pseudo
     CDS             complement(3924..4181)
                     /locus_tag="WGR04_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin, Grx4 family"
BASE COUNT         1053 a          981 c          980 g         1167 t
ORIGIN      
        1 tcgatcggct gccaatcaat cacttgatcc aacttcaata gtgggaaacg gtcgatgtgt
       61 ttggcaatca tggcttgtgc ggtttgctga aagaaggtgc tcatggaaca tcccctaaat
      121 gtcttggtgg gaatttaggg gattttgggg aattttgcaa aggtttccgc ctgaaacatt
      181 atgagatttc aggcggcatt ggattgctta gcggaatatt tttaaaaagg cttacgcgcc
      241 gtaaacgggg tatttattgc acaaagcagt tacttgttcg cggactttgg cgaggttggc
      301 ttcgtcttcg gggttagaca atacgtcggc aaccaagttc gccaatacgc gcgcgtcggc
      361 ttcgttaaaa ccgcgtgtgg tcatggcggc ggaaccgatg cggatgccgg aggtaacgaa
      421 gggtttttcc ggatcattcg ggatggcgtt tttgttgaca gtgatgtgcg ctttgcccaa
      481 agcagcttcg gcggctttgc cggtaatttt catcggttgc aggtcaacga ggaaaacgtg
      541 gctttcggtg cggccggaaa cgatgcgcaa accgcgttta accaactctt ccgccatggc
      601 ggctgcattg attttcactt gttttgcgta ttgtttgaac tcgggttgca atgcttcttt
      661 aaacgccacg gctttggcgg cgataacgtg catcagcggg ccgccttgca ggcttgggaa
      721 gatggaagag ttcaacgctt tttcgtgggt attgtcgcgg cacaaaatta cgccgccgcg
      781 aggaccgcgc agggttttgt gggtggtggt ggtaacgaag tcgcagaatg gcaccgggtt
      841 gggatattcg ccaccggcaa tcagtccggc gtagtgcgcc atgtcgacaa agaggtatgc
      901 gccgacttta tcggcgattt cgcggaattt tgcccagtcg atttgcaacg cgtaggcaga
      961 cgcacccgcc acaatcattt tgggtttgtg ttcgagcgcg aggcgttcga cttcggcata
     1021 atcgagcact tcgttttcat ccaaaccata agtaacggca ttgtagagtt tgcctgagat
     1081 attaacgctc gcgccgtggg tcaggtggcc gccgtgcgcc agagacatac ccaaaatggt
     1141 gtcgcctggt tttaaaacgg aagcgtacac ggcttggttg gcttgcgagc cggagtgcgg
     1201 ttggacgttg gcgtattgcg cgccgaacag ttttttgacg cggtcaatcg ccaattgttc
     1261 gacaatatcg acgtattcgc agccgccgta gtagcgtttg ccggggtagc cttcggcgta
     1321 tttgttggtc agctgggaac cttgcgtttc cattacggcg cagctgacgt agttttcaga
     1381 ggcaatcagc tcgacgtggt cttgctggcg ttggtcttct tgggcaatgg ctgctgccaa
     1441 atcggggtcg tattgtgcga gggtaacgct ttttgaaaac atgttctcgg ctcctttgtg
     1501 taatcagggt atcatgagtg ttttttgtat aaaaaatact tcaataccta aggtagatag
     1561 cccataatgc gtaaattttc tttggcatta tcaggtaatt tatttaacat gggtaattta
     1621 tttaacatgc tggtttttag cgtctcatca cctttattta gtacaagact aatcaaaagc
     1681 aatacattaa atggtaaatt ttcggcagtt tgtgcaaaat caatcaaata accagcacca
     1741 accacatctt tatcagccat aacccgctga tacaccactt gctaagacaa ctctcctgcc
     1801 ccaccatgtc ttaaaatcag atgaatggct tgtaatgctg tgtttggtgt ctcacaaatt
     1861 gccaaaatct gatcagaagc ttgcttgatg gcttgtattt ttgtttgatt actcacaatt
     1921 tccccctatt ttaataatta acttaaatgc ggtcaaattc acaaaataca agcttcacct
     1981 ctaatcaccc atcactcgac cttctcggcg tggatcggca ccaccaacca gcctgcttgg
     2041 ctcgataata atggcttgaa cacctgaatt tagctcacgc acatcagtct tatagcccaa
     2101 atcatttaat gcttgttgcc actggacggc ggttgtaccc gtttctagtt catagttacc
     2161 aaagcgattt aataaattgg gtgcactgat ggcatcttgg atatccatat tccagtcact
     2221 atgtgccaca atcgtcttag cgacatagcc aatgatacgg ctaccacctg gggagccgat
     2281 tgccatataa ggcttgcctg ctttaaatac gatggttggt gccattgagg agcgtggtct
     2341 cttgccgggc tcgacacgat tggcgacctg tttgccctgc tttattggct caaaactaaa
     2401 gtctgtcagc tcattattca gcaggtagcc atttgccatc aaagttgagc caaacgcatt
     2461 ttcaatggaa gtcgtcattg atagcacatt gcccgcctta tccacaattg atatatgact
     2521 ggtagaaggt aactcaatcg cttgtgagga cacccactca tgaataaaat cgcctgcaga
     2581 tacgctaggc aatgccttat ccgactgctc aagcagctgg ctgcgatgtt ttaggtagtc
     2641 tttagaaatc aactggcgaa tgggtactgg tacaaaatca gggtcgccca aatatacatc
     2701 acgatccgca aacgcaagcc tagaagcgtc gcccaagaga cgtaaacctt cagcatcata
     2761 ccccacctga ttgggtgaaa attcatttaa aatccccaaa atctgaccca cagcaatccc
     2821 acctgagctt ggtgcaccca taccgcatac ttcataaata cgataagtca cacaaacagg
     2881 cgggcgttcc accacttgat aatcagataa atcttgtaag gataattgac cggggttatc
     2941 cttagcattt tggacaactg aaacgatatt ttgggcatat ttaccagcat gcagaacttt
     3001 tgcaccttga gctgctaacg cctgaacact gtcagcaaat tctaaatttt tcagcaagct
     3061 gcctgcttgt agcggcacac cattcggcaa aaaataagcg gctgtttttg gatagcgtgc
     3121 caaatgctgc tgattttgct caaccgagat ggcaagcctt ggcgacacct caaagccttg
     3181 ttttgccaag cggatcggcg tatcaaataa ttttccccaa ggcaatacac cgtatcgctg
     3241 atgtattgtc tccatcagtt tagggatagc aggcgtaccc accgagcgac caccgaccac
     3301 cgcttccata aatttcaatg gttgaccatc tttatccaaa aataattccg gcgtcgcacg
     3361 catcggtgcc gtctcacgcc catcaaatgt ggtcaatgtt ttggcggtat tatcccaata
     3421 caacacaaat gcaccaccgc ccaagcctga cgactgtggc tctaccaagc ttagtgtcgt
     3481 ctgcaccgcc accatcgcat ctgcagcgct accgccttgc tttaagatat catagccagc
     3541 ttgtgttgct aatggattgg ctgacgctac cataaaatca cttgcaatca cctgcttttg
     3601 ttcggtcagt cccgttgcat gttcaggcgt gtgagcgtct gcacctgtaa tgacagcaga
     3661 atgagtatta accttacctt gattggcatg gatgacttga catccagaga ttgtcataga
     3721 cattatcaat gcagtcaata aatatgtttt agccacaagc actccttcgc ctgagtttga
     3781 ttgatgattc atacaaggca tgctgattat tgtatttaat atgactaaat aattcaatcc
     3841 aaactatcaa tcttgaccat caaaaaaaga ccgctaatgt catcagcagt cttttttgat
     3901 atttatttta agatattaag taatcagacc tttgggctat gctcttcaat gagtggtttt
     3961 agctcacctg attggtacat ttgtgataat atcactacca ccgattaact caccattaac
     4021 ccaaagctgt ggaaaggttg gccgattggc gatgagtggt agagtactgc gaatttctgg
     4081 gttttctagg atattaacaa aagcaaaggg tctgccaatt gggtcagcac ctctactgca
     4141 cgcgctgaaa atccacattg gggaaactgg ggcgtgcctt t
//
LOCUS       DAWFJR010000127         4176 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWFJR010000127 DAWFJR010000000
VERSION     DAWFJR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.127"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..470
                     /locus_tag="WGR04_001947"
     CDS             <1..470
                     /locus_tag="WGR04_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0795434.1"
                     /translation="FEDPVEPDESDFGGWRKGRCGRGAAGKVVVFDILKRNGRVYTVV
                     VDNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLDVSGFIHYRINHSKEFADRQNHIN
                     GIENFWNQAKRVLRKYNGIDRKSFPLFLKECEFRFNFGTPSQQLKILRDWCGI"
     gene            683..2218
                     /locus_tag="WGR04_001948"
     CDS             683..2218
                     /locus_tag="WGR04_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0795435.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSSLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            2215..2307
                     /locus_tag="WGR04_001949"
     CDS             2215..2307
                     /locus_tag="WGR04_001949"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ0795436.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(2343..3587)
                     /gene="lysA"
                     /locus_tag="WGR04_001950"
     CDS             complement(2343..3587)
                     /gene="lysA"
                     /locus_tag="WGR04_001950"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ0795437.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQNEKVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKYGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKDIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVNGGGYKLIRRR
                     ETLEQQMANELACLQAEHQNAV"
     gene            complement(3598..3768)
                     /locus_tag="WGR04_001951"
     CDS             complement(3598..3768)
                     /locus_tag="WGR04_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0795438.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QGKPQSAPPTQK"
     gene            3839..4162
                     /gene="cyaY"
                     /locus_tag="WGR04_001952"
     CDS             3839..4162
                     /gene="cyaY"
                     /locus_tag="WGR04_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ0795439.1"
                     /translation="MMTESEFIRASEALFEHIEGQIDENGWDFDCQFAGNVLTIEAGN
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDNRDFYDVLNEALSAASGEAV
                     KIAEL"
BASE COUNT          891 a         1028 c         1174 g         1083 t
ORIGIN      
        1 tttttgagga tcctgtcgag ccggacgaaa gcgatttcgg cgggtggcgt aaaggcagat
       61 gtggtcgtgg tgcggcagga aaagtggttg tcttcgacat tctgaaacgc aacggacggg
      121 tctataccgt tgtggtggat aatgccaagt ctgaaacgtt actccctgtc atcaaaaaga
      181 aaatcatgcc ggacagtatt gtttataccg atagtctgag cagctgcgac aagttggacg
      241 tgagcggttt tatccattac cgcatcaacc attccaagga gtttgcagac cgtcagaacc
      301 acattaacgg cattgagaat ttttggaatc aggcaaaacg cgtcttgcga aaatacaatg
      361 gaatcgatcg caaatctttc ccgctgttct tgaaagaatg cgaatttcga tttaacttcg
      421 gcacaccgtc tcaacagctt aaaatcctgc gggattggtg tgggatttag ggctaatcta
      481 gtacagcccc taaaattttt cgttttcaag ccttcaccgc ttgccatcag cgttaaattt
      541 ttttacgata agcacataga ttgtaaacaa tcggccacaa gccggtttgt tttttcagaa
      601 gacattatcc ctgtcagacg ctatttctat atatttcgcc tataatggct tgtttttaat
      661 aaataattca agaggtatca acgtgtctga ttccaagacg aaagaacgcg ccacattcgg
      721 cacgcgccgc gcgtttatga ttgccgccat cgggtccgcc gtcggcttgg gcaatatttg
      781 gcgtttcccc tatattgctt ttgaaaacgg cggcggcgcg ttcatcctgc cctatctggt
      841 cgcgcttctg acggcgggca tcccgctgct gctgctcgat tatgccatcg gccaccgtta
      901 ccgtggttct gcgcccttgg ctttccgccg cctcggacga tggtttgagc cggtcggctg
      961 gtggaacgtg atgaccaata tcgtcatctg catctattac gcggtaatta tcggttgggc
     1021 ggcaagctat acctattatt cggtcaacgc cgcctggggt gcggatccgc agggtttttt
     1081 ctttaaggac ttcctgcaaa tggcgggccc ggaagccttg ggtttggatt ttgtcggcaa
     1141 agtcgccggt cctttggcgg gcgtgtgggt ttttaccgcc gccattatgg cgttgggcgt
     1201 gcaaaagggc gtggcgcgcg cctcgtcgtt ctttatgccg ctgcttttgg tgatgttttt
     1261 gattatggtc ggcatttcac taaccctgcc gggtgcggca aagggcttgg acgcattgtt
     1321 tacgcccgac tggtcgaaac tcgccgattc caaggtctgg gtggcggcat acgggcagat
     1381 tttcttttcg ctttccatct gcttcggcat tatggttacc tattcttctt atttgaagaa
     1441 aaaaaccgac ttgggcggaa cggggctggt ggtcggtttt gccaacagca gctttgaact
     1501 gctcgcgggc atcggcgtgt ttgccgcatt gggctttatg gcgcaggcgg gcggtaaggc
     1561 ggtcaacgag gttgcctcag gcggcatcgg tttggcgttt atcgcctttc cgaccattat
     1621 caaccaggca ccgatgggct ggctgatcgg catattgttt ttcggttcgc tggtattcgc
     1681 cggcgttacg tcgatgattt ccatccttga agtgattgtg gcggcgattc aggacaagct
     1741 gaacatcggg cgcgtcaacg ccacgctgct ggtctgcatt ccgatgggca ttgtttccac
     1801 gctgctgttc ggtacggcga cggggctgcc ggttttggac gtgatggaca aattcgtcaa
     1861 cacctacggc attgttgccg ccggctttgt ttatgttgcc gccatcatca tcagcggcag
     1921 gctgccggaa ttacgcaagc acctgaacgc tttgtcctcc atccgcatcg gcggcttgtg
     1981 gacggtctgc gtcgtggtta ccgtcgtgat gctcggctat atgctgttta aagataccag
     2041 cagcctgatg gagaaaaatt acgaaggtta tccggatggt ttcctcagta ttttcggctg
     2101 ggggatgtcg gcggcgttgg tcgtgttcgg gctgctgctg tcgttgctgc cttggaaaca
     2161 cggtcaggat ttcaacgtca aagacgaaca cgaacatgaa caaggagaag aaaaatgagt
     2221 acttccgcca ttgtgatgat gattgtctca atcgtgataa tctggggagg gctgctgctt
     2281 tccctgttaa ggctgccgaa cgagtaagcc tttagagcgt taaaaatgcc gtctgaaccg
     2341 cttcagacgg cattttgatg ttcggcttgc aggcaggcga gttcgtttgc catttgctgt
     2401 tctaaggttt cgcgtcggcg gatgagtttg tatccgccac cgttaaccaa cacttccgcc
     2461 gcacggttgc gcgcgttgta attgctcgcc atactggctc cgtatgcgcc cgcgctgcgg
     2521 ataagcagca aatcgccttc ttcgcaggcg atggtgcggt ctttgccgag gaagtcgccg
     2581 gtttcacaaa tcgggccgac gatgttggcg gtcagaggct caatgtcttt ggtttcgacc
     2641 gcctcgatgt gatgataggc atcatatagg gcggggcgca ttaaatcgtt catcgccgca
     2701 tcgaccatca caaagttttt ctcttcgccg tatttgacga attcgacacg cgtcaacaac
     2761 gcgcctgcgt tgccgaccaa gctgcggccg ggctcaagaa tgagtttcag acggcgtgtg
     2821 ccgatcagtt tttgaaccgc ttgggcatac gcgcccaaat caggcacttt ttcgttttgg
     2881 taaacaatgc cgacgccgcc gcctaagtcc aaatgttcca aaacaatgcc ttcggcggca
     2941 agcgcgtcaa ccaaaatcaa aatgcgttcg caggcttcga ccagcgggct taagtcggtt
     3001 agttgggagc cgatgtggca gtcgatgccg atgattttca aattgggctg ttgtgcggca
     3061 tggcggtagg cttcgagcgc gtcggcgtag gcgataccga atttgttggc tttcaaacct
     3121 gtggagatgt aaggatgggt tttcgcatcg acatcgggat tgacgcgcag ggagacgggc
     3181 gcggttttac ccaaacgcgc ggcaactttc tgaatacggt cgatttcggg gatgctttcc
     3241 atattgaagc atttcacgcc tgcattcagc gcgaactcga tttccgcctc gcttttgcct
     3301 acgcctgaaa atatggtttt tgccgcgtcg ccgcctgccg ccaaaacgcg tgccaattcg
     3361 ccgccggaca caatgtcaaa accgctcccc aaagaagcaa agtgtttgat gatgctcagg
     3421 ttgccattgg ctttaaccgc atagcagacg agcggggaga gctcggcaaa ggcagtttgg
     3481 tagtgttcaa atgcttcggt cagcgcggat tggctgtaca cataaagcgg tgtgccgaat
     3541 gcttcggcaa ggcgggggta ggggacttgt tcgcaaaata gggtcatatt ttcgttttca
     3601 tttttgggtt ggaggggcgg attgcggttt gccttgaagc tgcaaaccgg tttggattac
     3661 gccgaaacgc gccttgtcgc cttctttggg caggtagagg tcgcctttgt aaccgcaggc
     3721 cgagagcagg agggcggttg ccgccgcaaa aaatacgccg tatttcatcg gtaaacttcc
     3781 ttcataagcg cgaatgtggc aagattcggc atcttaaaca aaaaacacgc aaaaagccat
     3841 gatgaccgaa agcgagttta tccgcgcgag cgaagcatta tttgaacaca tcgaaggcca
     3901 aatcgacgaa aacggctggg atttcgactg ccagtttgcc ggaaacgtcc tgaccatcga
     3961 agccggaaac ggcacgcaaa ttattgtcaa ccgccacacg cccaaccaag aattgtggat
     4021 tgccgccaaa agcggcggct accatttcgc cgagcaaaac ggcaaatggc tggcaacgcg
     4081 cgacaaccgc gatttttatg acgttttaaa cgaagccctg agcgcggctt cgggcgaagc
     4141 ggtaaagatt gccgaattgt aatttgagtg ttatca
//
LOCUS       DAWFJR010000128         4161 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWFJR010000128 DAWFJR010000000
VERSION     DAWFJR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.128"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(82..318)
                     /locus_tag="WGR04_001953"
                     /pseudo
     CDS             complement(82..318)
                     /locus_tag="WGR04_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            514..972
                     /locus_tag="WGR04_001954"
                     /pseudo
     CDS             514..972
                     /locus_tag="WGR04_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            872..1240
                     /locus_tag="WGR04_001955"
     CDS             872..1240
                     /locus_tag="WGR04_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ0795440.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            1309..2847
                     /gene="lnt"
                     /locus_tag="WGR04_001956"
     CDS             1309..2847
                     /gene="lnt"
                     /locus_tag="WGR04_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ0795441.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSTLLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            3111..3974
                     /gene="rpoH"
                     /locus_tag="WGR04_001957"
     CDS             3111..3974
                     /gene="rpoH"
                     /locus_tag="WGR04_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ0795442.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
BASE COUNT         1011 a         1295 c          952 g          903 t
ORIGIN      
        1 tatcgacctt gccgtcgggc aggatgtacc acgccaaatc cccgcctgaa gcacggtgtc
       61 ggactgcatt tctattcggc tatacgcctt ttcacgcagg ctgtaattga atttccacaa
      121 accgccgaac actgccgacg gacgcgcgac cagcatcagg aacgccgcaa tcgccaaggc
      181 ttccgccgcc ctgtccaaca cgttggcagg agaaaccagc agcaaaggca ttcccaaacg
      241 tgcggacaaa gtggtcgaaa ccacgctcag aaacaacagt gcgccacccg gcaggaacag
      301 actgcccatc caatccacgc taacgattct cccttaaaat tcatgaaatt aaaatcgaaa
      361 gactgtaaca cggtattttc ttttcacgaa actttacttc acgtccgcca cagcgttcag
      421 acggcatttt cctgtttcat gccgcctgaa accgcccaaa tccgcgttct cctatataat
      481 gtcggacttt gcgccgcatt tcaggattct ttaatggcat ttgcctcact atttaccctg
      541 ccggacgaca tcaccgccgt tttggacgat gttgccctga tgaccaaagc cgccgcaaag
      601 aaaaccgccg gcatggtcgg cgacgatttg acacccaatg ccaaccgggt tacgggcgta
      661 tcggcagaac gcgagttgcc gattgtttgg tcgatggcga aaggttcttt ttccaacaag
      721 ccggttttgg tgcctgcgcc cctgctgctt tccgccttcc tgccgcaact cattacgccg
      781 cttttgacgg caggcggcat ttatctgtgc tttgaaggcg tagaaaagct gctgcacaag
      841 tttctgcacc gacatgacgc gcatgctcgt aatggcggca atcggcatcg gcatgactgt
      901 tttggtgtac ggaatcgttg ccgtcatcgt caaactcgac gacttaggca tgctgctgat
      961 gcgccgccca caaacctttt cgcgcagttt ggggcaaatg ctgactgcct ttatgccttg
     1021 ctttatgcgc ggtttgagcg tggtcggcac gcttgccatg tttcttatcg gcggcgtact
     1081 ggtcgcccac aacctcggcc ttctgcacga tttcctgcac gcgcaacact gggacgctgg
     1141 ctgggcggag tacttcgcca acctcgttgt cgggctgctt tccggttcga ttgcctgcgc
     1201 ccccgccttg ccgctgatga atcgtttcgg caggcattga tttctttttc aaataccgat
     1261 gccgtttgaa agatgttcag acggtatctt ccgaacagac agatgaatat ggtttccaaa
     1321 ctggacaaat actggcagca ccccgccctc tactggcctt tgctcatcct ttttgccgcc
     1381 gccaccccct ttaccttcgc accctactac cacttttggc tgatgccctt gattttcggt
     1441 gccttcgtcc gcctcatcga actgcgtccg cgttttgctg tctcttccgc ctacctgttc
     1501 ggcctgaccg catacacgac acagttctac tggatacaca ccgccctgca cgacgtttcc
     1561 ggcctgcccg acctctatgc cgtaccgctg accttcctac tccccgccta ccttgccctt
     1621 tatccggcac tgtgtttctg gctgtggaaa aaatttaccc tgcctcgggg cataaaaatc
     1681 ggtttggtac tgcccatcct gtggacgctg accgagtttg cccgcgagcg tttcctgacc
     1741 ggattcggct ggggcgcaat cggctactcc caaatcaccc cggacagccc gctcgccggc
     1801 tttgccccat tgggcggcat ccacatggtt acactggcaa ccgcctttct cggtgtctgg
     1861 ctggttttgg cgagtgataa caccgcacgt tcgggcaaac gcctgcttcc aattatcctg
     1921 attgccgccc tgcttgccgc aggctacacc gcccgacaaa ccgacttcac ccgccccgac
     1981 ggcagccgca gcaccgtcgc cctgcttcaa ggcaacatcg accaaaccct caaatggcgt
     2041 gaagaccaag tcatcccgac catacagaaa tattacgaac aggtcggcaa aaccactgcc
     2101 gacatcgtca tcctgcccga aaccgccatc cccgttatgc gccaaaacct gccggaaaac
     2161 atactggcaa aatttgccga acaggcgcaa aacaacggca gcgcgctcgc cgtcggcatc
     2221 agccaataca cttcggacgg caacggttac gaaaacgccg tcatcaacct gaccggttat
     2281 caggaaaaca atcaggacgg catcccctac tacgccaaaa accacctcgt ccccttcggc
     2341 gaatacaaac cgctgccctt cctgaccacg ccgctttaca aaatgatgaa tatgcccctt
     2401 tccgacttcc gcaaaggcgg cggtaagcaa tccaccctgc tgatgaaaaa ccaaaaaatc
     2461 gccttcaaca tctgttacga agacggattc ggcgacgaac tgattgccgc cgccaaagat
     2521 gccaccctgc ttgccaatgc cagcaatatg gcgtggtacg gaaaatccaa cgccatgtac
     2581 cagcacctcc aacaatcgca ggcacgggct atggaactcg gacgctatat ggtccgcgcc
     2641 accaacaccg gcgcaaccgc catcatctcc cccaaaggca acatcatcgc ccaagcccaa
     2701 cccgatacgg aaaccgtatt ggaaggacac atcaaaggct atgtcggcga aacgccctat
     2761 atgaaaaccg gcagttcatg gtggttgatg ggcatattga ccctagccgc actgattctt
     2821 ttcatcttcc gaaacaaaga acactgatac ctgcacagcc cgtccggacg ggctgtcttt
     2881 tttcaaaccg gccgtttaca tacaactacg tatgtaatat aattctgaaa tagaattgaa
     2941 ccatcaggtt tacaaaatca atcaaacttg ccttacaaaa ttttcctgtc tttcaaacct
     3001 tagcgcatat ctcctgccaa acatacaaaa tacaagccaa gcagtgatat actgcatccc
     3061 aacacagcaa accgccgata cgcagtttca gccgaaagga aatgccccaa atgaataacg
     3121 ctttcgcatt acccgccatc caaagcggca acggcagcct cgaacaatac atccacaccg
     3181 tcaacagcat cccgatgctg tcccaggaag aagaaacccg cctcgccgaa cgccggataa
     3241 agggcgacct caacgccgcc aaacaactca tcctgtccca cctgcgcgtc gtcgtttcca
     3301 tcgcgcgcgg ctatgacggc tacgggctga atcaggcaga cctgattcag gaaggcaata
     3361 tcggactgat gaaggcggtc aaacgctacg agccaggtcg aggtgcacgc ctgttttcat
     3421 ttgccgtgca ttggattaaa gcggaaattc acgaattcat cctgaggaac tggcgccttg
     3481 tccgcgttgc caccaccaaa ccgcaacgca agctgttttt caatttacgc agtatgcgta
     3541 aaaacctcaa cgccctgtct cccaaagagg cacaagacat cgccgacgat ttgggtgtca
     3601 aactttccga agttctggaa atggaacagc gcatgacggg acacgacatc gccatcatgg
     3661 cagacaacag cgacgacgag gacagtttcg cccccatcga ctggcttgcc gaccacgatt
     3721 ccgaaccgag ccgacaacta tcgaaacagg cgcattacgc cctgcaaaca gaaggtttac
     3781 aaaacgcttt ggcgcaactg gatgacagga gccgccgtat tgtggaaagc cgctggcttc
     3841 aagacgacgg cgggctgacg ctgcaccagc ttgctgcaga atacggcgtc tctgccgagc
     3901 gcatccgcca gattgaagca aaagccatgc agaaactgcg tggtttcctg accgaagaag
     3961 ccgaagcggt ttgaaatata cgcaataaaa tacccgacaa attgttttgt cgggtatttt
     4021 atttggtaaa catcaatgcg gtttgtgcgt cgtgctgtac acgattttat ccgtccacgc
     4081 cgttgcaatt gccgaaatca aaaagcagac atgcaccaga cactgccgca tcagctgctt
     4141 ttccgacaga ttggcggaat t
//
LOCUS       DAWFJR010000129         4120 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWFJR010000129 DAWFJR010000000
VERSION     DAWFJR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.129"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(227..1420)
                     /locus_tag="WGR04_001958"
     CDS             complement(227..1420)
                     /locus_tag="WGR04_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ0795443.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNSGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLTIHTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLSQWSKWRADRMNDLAQTGADLVILSYGTNEAFNNNID
                     IADTEQKWLDTVRQIHDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(1417..2400)
                     /locus_tag="WGR04_001959"
     CDS             complement(1417..2400)
                     /locus_tag="WGR04_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ0795444.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYALSDGIKAFLSGETLPTAQDSGLEDMPSEAAASEAVPQTGET
                     EWKQDTDTAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLQKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAARAHHVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGEDR
                     HTDSVNVNGKSVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            complement(2411..3847)
                     /locus_tag="WGR04_001960"
     CDS             complement(2411..3847)
                     /locus_tag="WGR04_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ0795445.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIIVLYSSCVYLLGELLRSDRENTRRFWLGCGTAVSLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSIAYLVYCFRAPHAARFSWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTHQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLGGVYGYTFQLFLDFSGYSDLVIGMAMLLGFSLPKNFSAPL
                     RALNIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRDALCRLKYLAPLSWFVTFHFVCLSFVVFNTANPD
                     DAGAVFSALFANAGGWNAPQRADMLLLASFAAVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPISIILLLIIVLAPSGIPGFIYANF"
     gene            complement(3977..>4120)
                     /locus_tag="WGR04_001961"
     CDS             complement(3977..>4120)
                     /locus_tag="WGR04_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEZ0795446.1"
                     /translation="NVGIGSGALLGHWVTQYVGISRIGIAGMLAAAAGLWLCLNLNRH
                     IQT"
BASE COUNT          844 a         1046 c         1347 g          883 t
ORIGIN      
        1 aaaacggaag gcggcagttt atcatctttg ccttcagacg gcatatcaaa cgccgccttt
       61 gcgccgtatg ccggaggttc gcccccgtcc ttccgatgct tgaggcgaat gccggttgcg
      121 tccggcaaac cgtgcgcggg gtggctatgc cgtctgaagc aatatgaagg tatgcgtttc
      181 agacggcatc cccctgttgc ggaaataccg tccgcctcct gtccgattat tgcctgattg
      241 cagcggcgcg gacgagttct tcgaggctgt cggcgagcat ttccgccgcg cgccggtagc
      301 ctttggcgga gaagtgtacg ccgtctttgg cggcccatcc ttggttgagc cagtttttca
      361 tgctgcatat gccgcccatg gcgttttgcc aagaccagaa catggtttgt ccctgccggg
      421 cgacgcgccg ctgcatctgt tggacttcgg tcaggcggac ggggcgtgtg ccgcatacgc
      481 cgagcgtgtt tttcagggat tcgggtgcgc cgatgatgag gatgccggcg gcaggcaggc
      541 tgtcgtggat ttggcggacg gtatccagcc atttttgttc ggtatcggca atgtcgatgt
      601 tgttgttgaa ggcttcgttg gtgccgtagg aaaggataac caaatcggcg ccggtttggg
      661 caaggtcgtt catacggtcg gcacgccatt tcgaccattg ggacaattgt gcgccgttga
      721 tgcccatcgc ggaaacggta atgccgccgg cggggttttc gatgttgatg aagccgatgt
      781 cccacggcat ttcggtgtgt atggtcaggg gcagtgccgc gcccgtatcc agtacctgcc
      841 agccgccgcc gttggcggag acggtgttgc cgttgacggt cagggtttgt tcggcgagca
      901 ggggtttggc aaacagggaa atgcgctgtt tgccggtctt gccgtccgat gcggtcaggg
      961 tcatgctgcc gccgctgccg gtgtgggcga ggatgccgcc gagcgggaag tctccgctat
     1021 tgttcctgct ggtgaggctt tgccagttac cgttgtgccg gacggccgcc atgcgctgcc
     1081 ctttgacgtt ggcggggtaa acccagccta tgccgccgtc gccccaagtt ttttgcaggc
     1141 gtttgcgcag gctgtcggta aagaagtcgc cggcggtatg cgagtcgccg atttgcagga
     1201 tgcggaaggt ctcgccgctg ccttgtgcga cggatcggag ttttttcatc caaggcgagg
     1261 cggcggcgtt gccgtagtcg gtcaagagcc cgtttgtatc ggtgtagggg gcggacgggg
     1321 aaacggtaac ggtgtcgagg ctgacggagg cgacgggcag ggcttctgcc tgcgtggcgg
     1381 cgaacagggc ggaaaatgcg atgaggtgtt tggggttcat ggctgtgtac ttgatggttg
     1441 cgtacttggt tcaaaaacga ttttttccat tatttttgcc gccagcagtt tttgtccttc
     1501 ggcggtaaag tgtatgccgt ccttgctgcg gtagcggacg gatttgccgt tgacgttgac
     1561 ggaatcggtg tggcggtctt ccccgccgct cagggtgtgc gcggtgggaa tcaggatgat
     1621 tttgccttcc aagtgttccg aaagcagttt gtcgaggtag cgcatctgcc cgtcgagctt
     1681 ggcttttttc atgtagggga tgccgagcca gacgacttgg acgtggtgcg cgcgggcggc
     1741 ttcgaggatg cggtcgacgc gtttcaggta ttcttgcgcc cattcgtcgg aagcgaattt
     1801 gaggtagcgt ttgccgacgg gaaaatccca cgggtcgttc ggaccgagga agacggcgag
     1861 cacgctgatt tcggggtgtt tttgcagggt ttcttcaatc gttttcggcc agtcgaagaa
     1921 tgaggggtag gacagccccg tgctttgttt gctgaggttg acggattcga tgccgtattg
     1981 ctgtttcagg cttttttgca cgaagggtgc aacgccctgc atcagcgaat cgccggcgaa
     2041 aaagactttg tcgccgctgc ggacggcggc ggtgtcggtg tcttgtttcc attctgtttc
     2101 accggtttga gggacggctt cggatgcggc ggcttcagac ggcatatctt ccaaaccgct
     2161 gtcttgggcc gtcggcagtg tttcgccaga caggaaggct ttgatgccgt ctgaaagggc
     2221 gtaggcgttt tcttgcaacg ccgcaccgct ccgccaccat ccgtaggcgg cgagcggttc
     2281 gagcggactg ttgcggtggt aggtctgctg ccagtaggcg ttgatggggt tttggctgaa
     2341 ccacacggca atcagggcag acatcaatat ggaggcgaaa agggaaagaa agtttttcat
     2401 gtccaaaccc ttaaaaattg gcataaataa agccgggtat ccccgagggg gcgaggacga
     2461 taatcagcag caggatgatg gaaatcggga taaaccacag ccacatcggg attttttcca
     2521 aacctttgac cgcgccgtcg aaagcgcgtt gcaggtaagg gtagagcagc atcacggctg
     2581 caaacgaggc aagcagcagc atgtctgccc gttgcggcgc gttccagccg ccggcattgg
     2641 caaagagcgc gctgaaaacc gcgccggcat cgtcgggatt ggcggtattg aagacgacaa
     2701 agctaaggca gacgaaatgg aaggtaacaa accacgagag cggcgcgagg tatttcagac
     2761 ggcatagcgc gtcgcgtccg aaatagcggt cgcccgtgtt gagcagcacc agtgccgtgc
     2821 cgtgcagcgc gccccaaatg aggaagttcc agccgtagcc gtgccatatg ccggagagta
     2881 ccattgccgc catcaggttg agctgagtcc gtaaaaagcc ttttttgctg ccgcccaagg
     2941 ggatgtagat gtagtcgcgt atccaggtgg aaaggctgat gtgccatttg tcccaaaatg
     3001 cgcggatgtt taaagcacga agcggtgcgg agaagttttt gggcagacta aagcccagca
     3061 gcattgccat gccgataacc aaatcggaat atccggaaaa gtccaggaag agttggaagg
     3121 tatagccgta cacgccgccc aatacgcccc agccgtcgaa ttgggcggga ttttcaaata
     3181 cgggcgatac ccaattttcc gccagcatcc ccgccagcca ccattttttg gcaataccca
     3241 gcagaatcag ggaaacggcg agtgcggggc ggacgggtga gcgtggttgg tgggtacgga
     3301 tttgcgccaa tgcgcccgcc tgctcgccgt ccgcgctttt gaatgcggcg gcgcggataa
     3361 tcgggccgga ggtaacggtg gggaaaaaac tcaggtgcag cagcagctcg tgccagctga
     3421 aacgcgcggc gtgcggcgca cggaagcagt aaaccaaata ggcgattgac tggaaggtgt
     3481 aatacgaaag ccccagcggc atcaggatgt cgattgcgcc gccttgtccg gcatattgtg
     3541 caatcagcgg gcggaaaaaa tcgaaatatt tgaaaaagcc caagacggtc agcgagacgg
     3601 cagtgccgca ccccagccaa aagcggcgcg tattttcgcg atcggaacgg agcagttcgc
     3661 ccaaaaggta cacgcaggag gaataaagga cgatgattgc cgcaaatacg gggccgatat
     3721 ggtagagcca gcccataccg gcagccaaaa gcagcaggtt ttggacggac gggtatttcg
     3781 ccaagcccca gtaaatcggc aggaaggcga gaaagaacag tgcgaactcg acagacagca
     3841 gcggcataaa ccatccttgg aataagaaaa taggcgttaa aacacggcaa agcccgttat
     3901 tttaagcagg ggcggatatt tcttcaatca aaatgccgtc tgaagggcgg gaggggcttc
     3961 agacggcatc gcgcggtcag gtttggatat ggcggttcaa gttcaggcac agccacagcc
     4021 cggccgccgc cgcaagcata ccggcgatgc cgatacggga aatgccgaca tattgcgtta
     4081 cccaatgtcc caacagtgcg ccgctgccga taccgacgtt
//
LOCUS       DAWFJR010000130         4120 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWFJR010000130 DAWFJR010000000
VERSION     DAWFJR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.130"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            43..675
                     /locus_tag="WGR04_001962"
     CDS             43..675
                     /locus_tag="WGR04_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ0795447.1"
                     /translation="MENTNRVSEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFSGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAVSLFAISDDEDEIVAYLSEHGLQTA"
     gene            747..2117
                     /gene="purB"
                     /locus_tag="WGR04_001963"
     CDS             747..2117
                     /gene="purB"
                     /locus_tag="WGR04_001963"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ0795448.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIVEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKER
                     FSGVPEVAAASEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMSNAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIESLEIPVEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            2198..4066
                     /locus_tag="WGR04_001964"
     CDS             2198..4066
                     /locus_tag="WGR04_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0795449.1"
                     /translation="MQTVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTASSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLVKDIPNV
                     HWVDAQKYLPKNTVEIHGCYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRDGALQ"
BASE COUNT          936 a         1117 c         1062 g         1005 t
ORIGIN      
        1 tttttgacgg tgtacaatcg ctgtttttga acggaggatg gaatggagaa tacaaaccgt
       61 gtgtcggagc aggcacgtta tgatgccgaa cgccggcagg cagacgaagc attggcgggc
      121 gtgtttccgg cagtcagtat cttcggcagc gcgcgcacgc cgcagaatca tgcggattat
      181 gcgttcgcct gccgtctggc gcggcggctg tcggattcgg gcattgccgt catttcgggc
      241 ggcgggccgg ggattatgga ggcggcaaac aagggcgcgt tttcagggaa gtcggtttcg
      301 gtggggctga acatcgtttt gccgcacgag cagaaaccga atccgtatca ggacatcgcc
      361 ttgcggtttt cccgttttgc cgaacgcaag gcggtgtttt tccgctattc ccaagcatat
      421 gtcgtgatgc cgggcggctt cgggacgctg gacgaattgt ttgaaatcct gaccttggtg
      481 cagacgggca aagtgccgcc gcgtccgatt gttttggtcg gaaaggcgtt ttggtcgggc
      541 ttggcggagt ggataaacgc gcagcttttg gcgcgcgggc tgatttccga aggggcggtc
      601 tctttgtttg ccatatcgga cgatgaagac gaaatcgttg cgtatctgtc ggaacacggg
      661 cttcagacgg catagcgtcc tgagagtgat gtataattgc aaacaattta acaatttttg
      721 atgtctttcc cgaacaggat gccgaaatga tcaaccccat cgcctcgctt tcccctttag
      781 acggccgtta tgcccaatcc gttgaagcat tgcgcccgat tttttccgaa tacggcctga
      841 tgaaagcgcg cgtcaaagtc gaattaaact ggctcaaagc cctcgccgcc gagccgaaga
      901 ttgtcgaagt gccgcccttc agtgccgaaa cgcttgccga aatcgacacg gtgattgaaa
      961 acttttcatt ggaagacgcg gccgccgtca aagccatcga agccaccacc aatcacgatg
     1021 tcaaagccat tgaatattgg cttaaagaac gcttctccgg cgtgcctgaa gtggcagccg
     1081 ccagcgagtt catccacttc gcctgcacca gcgaagacat caacaacctg tcccacgctt
     1141 taatgctgca agaagcgcgt gaggctgttt tgctgccgaa gctggccgaa atcatcgaaa
     1201 aactgaccgc tatggcgcac gaccttgccg ccgtcccgat gatgagccgc acccacggac
     1261 agcccgccac gccgaccact ttgggcaaag aaaccgccaa tgtcgtgtac cgcctgcaac
     1321 gccagtttaa aaacctgcaa gcgcaagagt tcctcggcaa aatcaacggc gcggtcggca
     1381 actacaacgc ccatatggtc gcctatcctg atgtagattg ggaaacccac tgccgcaact
     1441 tcgtcgaaat cagcctcggt ctgaccttca acccctacac catccaaatc gaaccgcacg
     1501 actatatggc ggaattcttc caaaccctca gccgcatcaa cacgattctg atcgacttta
     1561 accgcgacgt ttggggctac atttctttgg gttacttcaa acaaaaagtc aaagcaggcg
     1621 aagtcggttc ttccactatg ccgcacaaag tcaaccccat cgactttgaa aactccgagg
     1681 gcaacctcgg catgtccaac gccgtattgg gctttttgtc cgaaaaactg ccgatttccc
     1741 gttggcagcg cgacctgacc gacagcactg tcttacgcaa tatgggcgtg ggcgtgggct
     1801 atgctgtgtt gggtttcgcc gcccacctgc gcggcctgaa caagctcgaa cccaaccctg
     1861 ccgcccttgc cgccgatttg gatgccactt gggagctgct cgccgagccg attcaaaccg
     1921 taatgcgccg ttacggtgtc gccaatcctt acgaaaaact gaaagacctg acgcgcggca
     1981 aaggcggcat cacgcccgaa gtgctgaaag gctttatcga atcgttggaa attccggtag
     2041 aagccaaagc aaaattgctt gagctgactc ctgcactgta tgtgggcaag gctgaagcgt
     2101 tggcgaaacg gatttgagcg tttactgaaa ccgatgccgt ctgaacgcgc gttcagacgg
     2161 catttttaag ataacgggac atacgggggc gatattgatg caaactgtcc gatacaggcc
     2221 tgaaattgac ggattacggg ctgtcgccgt gctatccgtc attattttcc acctgaataa
     2281 ccgttggctg cccggaggat tcctgggggt ggacattttc tttgtcattt cgggattcct
     2341 cattaccggc atcattcttt ctgaaataca gaacggttct ttttctttcc gggattttta
     2401 tacccgcagg attaagcgga tttatcctgc ttttattgcg gccgtgtcgc tggcttcggt
     2461 gattgcctct caaatcttcc tttacgaaga tttcaaccaa atgcggaaaa ccgtggagct
     2521 ttctgcggtt ttcttgtcca atatttatct ggggtttcag caggggtatt tcgatttgag
     2581 tgccgacgag aaccccgtac tgcatatctg gtctttggcg gtagaggaac agtattacct
     2641 cctgtatccc cttttgctga tattttgctg caaaaaaacc aaatcgctac gggtgctgcg
     2701 taacatcagc atcatcctgt ttttgatttt gactgcctca tcgtttttgc caagcgggtt
     2761 ttataccgac atcctcaacc aacccaatac ttattacctt tcgacactga ggtttcccga
     2821 gctgttggca ggttcgctgc tggcggttta cgggcaaacg caaaacggca gacggcaaac
     2881 agcaaatgga aaacggcagt tgctttcatc actctgcttc ggcgcattgc ttgcctgcct
     2941 gttcgtgatt gacaaacaca atccgtttat cccgggaatg accctgctcc ttccctgcct
     3001 gctgacggca ctgcttatcc ggagtatgca atacgggaca cttccgaccc gcatcctgtc
     3061 ggcaagcccc atcgtatttg tcggcaaaat ctcttattcc ctatacctgt accattggat
     3121 ttttattgct ttcgcccatt acattacagg cgacaaacag ctcggactgc ctgccgtatc
     3181 ggcggttgcc gcgttgacgg ccggattttc cctgttgagt tattatttga ttgaacagcc
     3241 gcttagaaaa cggaagatga ccttcaaaaa ggcatttttc tgcctctatc tcgccccgtc
     3301 cctgatactt gtcggttaca acctgtacgc aagggggata ttgaaacagg aacacctccg
     3361 cccgttgccc ggcgcgcccc ttgctgcgga aaatcatttt ccggaaaccg tcctgaccct
     3421 cggcgactcg cacgccggac acctgcgggg gtttctggat tatgtcggca gccgggaagg
     3481 gtggaaagcc aaaatcctgt ccctcgattc ggagtgtttg gtttgggtag atgagaagct
     3541 ggcagacaac ccgttatgtc gaaaataccg ggatgaagtt gaaaaagccg aagccgtttt
     3601 cattgcccaa ttctatgatt tgaggatggg cggccagccc gtgccgagat ttgaagcgca
     3661 atccttccta atacccgggt tccaagcccg attcagggaa accgtcaaaa ggatagccgc
     3721 cgtcaaaccc gtctatgttt ttgcaaacaa cacatcaatc agccgttcgc ccctgaggga
     3781 ggaaaaattg aaaagatttg ccgcaaacca atatctccgc cccattcagg ctatgggcga
     3841 catcggcaag agcaatcagg cggtctttga tttggttaaa gatatcccca atgtgcattg
     3901 ggtggacgca cagaaatacc tgcctaaaaa cacggtcgaa atacacggct gctatcttta
     3961 cggcgaccaa gaccacctga cctatttcgg ttcttattat atggggcggg aatttcacaa
     4021 acacgaacgc ctgcttaaat cttctcgcga cggcgcattg cagtagcctg ccttgccgtc
     4081 cgatatcgtt tgtgccgccg tttgcctttc ggggcggcgg
//
LOCUS       DAWFJR010000131         4104 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWFJR010000131 DAWFJR010000000
VERSION     DAWFJR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.131"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(173..286)
                     /locus_tag="WGR04_001965"
                     /pseudo
     CDS             complement(173..286)
                     /locus_tag="WGR04_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            426..501
                     /locus_tag="WGR04_001966"
     tRNA            426..501
                     /locus_tag="WGR04_001966"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:460..462,aa:Glu,seq:ttc)
     gene            complement(1011..1208)
                     /locus_tag="WGR04_001967"
     CDS             complement(1011..1208)
                     /locus_tag="WGR04_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795450.1"
                     /translation="MPSENRSAGVCGALYAVLQDKAQGRAAGCIVATVFLPDGVKNTV
                     ATPTDNEKREQCRLNRVLRRA"
     gene            1269..2075
                     /gene="thiD"
                     /locus_tag="WGR04_001968"
     CDS             1269..2075
                     /gene="thiD"
                     /locus_tag="WGR04_001968"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ0795451.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITTVT
                     AQNTLGVSAVHLVPTETISAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGMHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKSLDVCEAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            complement(2125..2994)
                     /gene="tehB"
                     /locus_tag="WGR04_001969"
     CDS             complement(2125..2994)
                     /gene="tehB"
                     /locus_tag="WGR04_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ0795452.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKVENLPEVLLSGYSSEEGEWVCL
                     NVLQGDVEVRAPDGSAEVWSADGGDCVFAPQQVFSVKPKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAAQDTVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKRPE"
     gene            3108..3545
                     /gene="rnhA"
                     /locus_tag="WGR04_001970"
     CDS             3108..3545
                     /gene="rnhA"
                     /locus_tag="WGR04_001970"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ0795453.1"
                     /translation="MNQTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDVLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
BASE COUNT         1034 a         1176 c          981 g          913 t
ORIGIN      
        1 aatccgccgc cgtctgaaaa tttaaaagtt ctagattccc gctttcgcgg gaatgacgaa
       61 gtttcagacg gcatcgcccg cctgttttga tatggcggca ccccccccga caaaaaaaac
      121 aatccggaac gcatctgacc gttccggctt attttcaggc gaatccgccg catcagaaca
      181 tactgcgcac gcccatattg acctgccaag tctggcgcat cgtgtgcatc gaagaccttt
      241 gcgcctcaaa ataaagctgc cttccgttgt cggcattacc acgcaaaaaa atgaattgct
      301 tgatattcca atgtttttta tatgttttta tattgtgatg cgatcagaca agcgcccccc
      361 tgacatttgt ttagacggca tcgtattgct aaatttctat aagtatgtat aatgtccgtt
      421 tccacgcgcc catcgtctag aggcctagga cactgccctt tcacggcggc aaccggggtt
      481 cgaatccccg tgggcgtgcc aattcaaaaa cctgcttgtt tcaagcaggt ttttttatta
      541 tgaatcgtca ttcccgcacc cttcgtcatt cccgcgaaag cgggaatctg gagcgtaggg
      601 ttgaagaaac cgttttatcc gataagtttc cgtgccgaca ggtctggatt cccactttcg
      661 tgggaatgac gagtctttta taacctttaa ggctgctctg cccaatggtt tggattcccg
      721 cttatgcggg aatgacgggg cggggcgatg ccgtctgaaa cttcgtcatt cccacaaccc
      781 ttcgtcattc ccatgaaaac gggaatctag aacttctgaa ttttcagacg gctttataaa
      841 tattgccgct gccccaaggg ctagattccc gcctgcgcgg gaatgatgat ttggaaatca
      901 cccgaaaccc aaaaacaact gaaaccgaac acactggatt cccgctttcg taggaatgac
      961 ggaattttag gtttctgttt ttgtgggaat gacgaataaa gcgtgccggt ttatgctcgc
     1021 cgcaacacgc ggttcagacg gcattgctct cttttttcat tatcagtggg tgtagcaact
     1081 gtatttttca ccccgtcggg caaaaataca gttgctacga tgcaccccgc cgccctgccc
     1141 tgtgccttgt cctgcaatac ggcatataat gcaccacaaa cccccgcgct gcggttttca
     1201 gacggcatcg ccgtgctttt ttacaggcat tagccctttt tatcggacac aatattaagg
     1261 aggaacaaat gaaaagctct tttgtgcaaa cgcttaccat cgccggttcg gattcgggcg
     1321 gcggtgcggg cattcaggcg gatttgaaaa cgtttcagat gcgcggcgtg ttcggaacgt
     1381 gcgtcatcac caccgttacc gcgcaaaata ccttgggcgt gtcggcggtt catctcgtcc
     1441 cgaccgaaac catctccgca caaatccaag caatcaggga agactttgac atccgcgcct
     1501 acaaaatagg aatgctcggc acggcggaaa tcatcgaatg cgttgccgac aagctgaaac
     1561 actgcgactt cggcaggcgc gtacttgatc ctgtaatgat tgccaaaggc ggcgcgccgc
     1621 tgttgcagga ttccgccgtt gcggcactga cgcgcctgct gcttcccgat acggatgtat
     1681 tgacccccaa cctgcccgag gcggaagccc tgaccggcat gcatatcgaa aaccgtaaag
     1741 atgcggaacg tgcggcaaaa atcctgcttg attacggtgt caaaaatgtc gttatcaaag
     1801 gcggacattt gaacggcagc acaagcggac gctgcacgga ttggctgttt acgcaaaacg
     1861 aaacgctgga attggacagc ccgcgctttc cgaccgccca cacgcacggc acaggctgca
     1921 cgttttccgc ctgcattacc gccgagttgg caaaaagctt agacgtttgc gaagccgtac
     1981 agactgccaa ggcctacatc acggcggcaa tctcaaaccc tttggaaatc ggcgcgggac
     2041 acggtccggt caatcattgg gcgtatcggg actagacgta aaaatgccgt ctgaaacaaa
     2101 atgttcagac ggcatttttg aagattattc aggccttttc gccagcatgg ttacaaattt
     2161 aaaccgtatc ggattgccgt tttcgtcttt ggcgtgcatc gcgcccaatt cttctttata
     2221 ttcgaccagc tcccaatccc gataataatc cttcagttct ccctctttaa atttgaaggg
     2281 gaacggcatc gggcagggga aatccgccgt atccattgcc gatacaatca agttgtaccc
     2341 gcctgccgcc gtatgcgcct gcatatcggc aatcacgtca ggcacgcgct gcggcatcaa
     2401 gaacatcagc accactgttg ccacaatata atcaaactcg ccctgtaaag cggcggcgtt
     2461 caaatcatat tccagcgtgc ggacgttcaa gccctccgcc tctgccagct ccgccacgtt
     2521 tgccaaggcg gcgggattgt gatcgactgc agtaacttca aaccccttca aaccgagaaa
     2581 cagcgcgttg cgcccctgtc cgcagcccat atccaacgcc ctgcccgccg gtacggtatc
     2641 ctgtgccgcc gcgaccgcag aatgcgtggc actcatcccg tattttttgt gaaaatagtc
     2701 tgccgccgcg caatacagcg acaaacggat ttcggcatcg tccgttttcg gtttgacaga
     2761 aaacacctgc tgcggcgcaa acacacaatc gccgccgtct gccgaccaaa cttctgccga
     2821 cccgtccggt gcacgaactt cgacatcgcc ctgcaacaca ttcaggcaga cccactcccc
     2881 ttcctcagac gaatagcccg acaacaaaac ttccggcagg ttttccactt tccatacagg
     2941 catctgtccg aaacaaaaca actcgccact ttgacccact atccgctcct tcatattcaa
     3001 aaataaagtt gcacattata tgcctatttt aatccgccgc aatctttcag acggcacggc
     3061 gcgcaaaccg cttataatca cgccggacac cacacaaagg cacaataatg aaccaaaccg
     3121 tttaccttta caccgacggc gcgtgcaaag gcaatcccgg cgcgggcggc tggggcgtgt
     3181 tgatgcgcta cggcagccac gaaaaagaac ttttcggcgg cgaagcgcaa accaccaaca
     3241 accgcatgga gctgaccgcc gtcatcgaag gactgaaatc gctcaaacgc cgctgcaccg
     3301 tcatcatctg caccgactcg caatacgtca aaaacggcat ggaaaactgg atacacggtt
     3361 ggaagcgcaa cggctggaaa accgccgcca aacagcccgt caaaaacgac gacttgtgga
     3421 aagaactcga cgttctggtt gggcagcacc aagtcagctg gacttgggtt aaaggacacg
     3481 cgggacacgc ggaaaacgaa cgcgccgacg atttggcaaa ccgtggcgca gcgcagtttt
     3541 cctgactgcc gctccggcaa aaatgccgtc tgaaaccgct aatgggcttt agacggcatc
     3601 gtcctccacc gtcattcccg cgcaggcggg aatccaaacc gtcgggcaac ggcaatattc
     3661 aaaagccgtc tgaaaattta gaggctctag attcccgctt ttgcgggaat gacgaaaagt
     3721 tgcggacagc agaggaatga cgaggtttca gacggcattg ggggctttgt cgggcttcag
     3781 gcggcatccg accgccccgt cattcccgcg aaagcgggaa tctaaaaacc caacgctgca
     3841 aaaatttatt tagaaacaac tgaaaccgct ccaccgtcat tcccgcgcag gcgggaatcc
     3901 agtccgtcgg gtaacggcaa tattcaaaga ttatctgaaa gtttgaagtt ctagattccc
     3961 attttcacgg gaatgacgaa aagttgcagg aatgacggag tttcaggcgg catccgaccg
     4021 cccagtcatt cccgcgaaag caggaatcta aaaatccaac gctgcaaaaa tttatttaga
     4081 aacaactgaa accgctctgc cgtc
//
LOCUS       DAWFJR010000132         4086 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWFJR010000132 DAWFJR010000000
VERSION     DAWFJR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.132"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            282..1403
                     /gene="dnaJ"
                     /locus_tag="WGR04_001971"
     CDS             282..1403
                     /gene="dnaJ"
                     /locus_tag="WGR04_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ0795454.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1599..3617
                     /locus_tag="WGR04_001972"
     CDS             1599..3617
                     /locus_tag="WGR04_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ0795455.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDIEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSI
                     VVISLVLLLVGESGGLMADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIAADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNADRIGTLFAAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMIIFARRVLKAGR"
     gene            complement(3661..>4086)
                     /gene="rfbC"
                     /locus_tag="WGR04_001973"
     CDS             complement(3661..>4086)
                     /gene="rfbC"
                     /locus_tag="WGR04_001973"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ0795456.1"
                     /translation="VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAIDMRE
                     GSPTFGKWAGATLSAQNQCQLWIPEGFAHGFCVLGDAAEVVYKCTDYYKPEAEQVLIW
                     NDPTVGIGWPLQTAPLLSPKDLAGKTWAQAEKLRLTLSR"
BASE COUNT          863 a          936 c         1212 g         1075 t
ORIGIN      
        1 ggataaaaaa taaaataggg ggaagtctgc agccgcatca aatgccgtct gaacatgcgt
       61 tcgggcggcg tttttataac aaaaacactt catggcggtt ggttttatgc ctgtctaagt
      121 ttttgtgtcg tgcatacctg aagatttcag acggcatcgg tttatgctgt ctgaaaagtg
      181 tattccgttt cagtttgtaa gctatggcag tctgtttgtc ttgtgttttg cgcaattgcc
      241 cttattttga gccgtgattt tattttgaat tagatgaaaa aatgagtaat caagattttt
      301 atgcgacgct gggtgtggca agaacagcta ccgatgatga gattaaaaaa gcctaccgga
      361 aattggcgat gaaataccat cccgaccgca atcctgacaa taaagaggcg gaagagaagt
      421 ttaaagaagt acaaaaggcg tatgaaactt tgtccgacaa ggaaaagcgc gctatgtacg
      481 accagtatgg tcatgcggcg tttgaaggcg gcggacaggg gggcttcgga gggtttggcg
      541 gatttggcgg tgcgcagggt tttgactttg gggatatttt cagccaaatg tttggaggcg
      601 gttcggggcg cgcccagcct gattatcagg gtgaggacgt tcaagtcggt atcgaaatca
      661 cgcttgaaga agccgcaaaa ggtgtgaaga aacgcatcaa tattccgact tatgaagcgt
      721 gtgatgtctg taacggcagt ggcgcgaaac cggggacatc cccggaaacc tgcccgactt
      781 gcaaaggttc gggtacggtg cacatccagc aggcgatttt ccgtatgcag cagacttgtc
      841 cgacctgcca cggtgcgggc aaacacatta aagaaccttg cgtcaaatgc cgtggcgcgg
      901 ggcggaataa ggcggtcaag acggtggaag tcaatattcc cgccggtatc gatgacgggc
      961 agcgtatccg tttgagcggc gaaggcggac cgggtatgca cggtgcgcct gccggcgacc
     1021 tgtatgtaac cgttcgcatt cgggcgcata agattttcca acgcgacggt ctggacttgc
     1081 attgcgaact gccgatcagt tttgccacgg ctgctttggg cggggagttg gaagtgccga
     1141 ccttggacgg aaaggtcaag ctcaccgtcc ccaaagaaac ccaaaccggc aggaggatgc
     1201 gcgtgaaggg taagggtgtc aaatctttac gcagcagcgc gaccggcgat ttgtactgcc
     1261 atattgttgt cgaaacgcct gtcaatttga ccgaccgtca aaaagagctt ttggaagaat
     1321 ttgagcggat ttctaccggc ttggaaaacc aaacaccgcg caagaaatcg tttttagaca
     1381 agctgcgcga tttgtttgat tgattttaag gttcggaaac aagcagccgt atcggggaat
     1441 ctccctgata tggctgtttt tgtttgttta aaaatagttt ttattttcaa tggggtatgg
     1501 ggcagggtgg gataactgtt tttaactgtt ctttttaaaa cttgacatca tggcgcgatg
     1561 ccaacaatat gcgggaatct gttgtcaaag gaagaataat gaataaatct ttatccggtt
     1621 cggtagaaga ataccgcgaa ctgacgctcc gaggcatgat actcggtgca ttgattactg
     1681 taatttttac tgcgtccaac gtttacctcg gtttgaaagt cggactgacc tttgcctcgt
     1741 cgattccggc ggcggtaatt tcgatggcgg ttttaaagtt tttcaaaggc agcaatattt
     1801 tggaaaacaa catggtgcag acccaagctt cggcggcggg tacgctttcg accatcatct
     1861 tcgtcctgcc cggtttgctg atggcgggct actggagcgg tttcccgttc tggcagacga
     1921 cgcttttatg tattgccggc gggattttgg gggtgatttt caccattccg ctgcgttacg
     1981 cgatggtggt gaaaagcgat ttgccttatc cggaaggtgt ggcggctgct gaaattttga
     2041 aagtgggcgg tcatgaagaa ggggataacc gtcagggcgg cagcggcatc aaagagctgg
     2101 cggccggcgg tgcgttggcg ggattgatga gcttttgcgc cggaggtctg cgtgtgattg
     2161 ccgatagcgc gagttattgg tttaaaagcg gtacggcaat tttccaactg ccgatgggct
     2221 tttcactggc attgttgggc gcgggctatt tggtcggact gacgggcggt atcgccattc
     2281 tgttgggcat ttcgattgct tggggcattg ccgtgccgta tttttcatcg cacatcccgc
     2341 agccttccga tatagaaatg gcggcgtttg cgatgaagct gtggaaggaa aaagtacgct
     2401 tcatcggcgc gggaaccata ggtatcgcgg cggtttggac gcttttgatg ctgctcaagc
     2461 caatggtgga aggcatgaag atgtcgttca agagttttgg cggcggtgcg cccgcaacag
     2521 aacgcgctga acaggatttg tcgcctaaag ccatgatttt ctgggtattg tctatgatgt
     2581 ttattctagg cgtgtcgttt taccacttta tcggcgattc gcacattacg ggcggcatgg
     2641 cttggctttt ggtggtcgtt tgcacgcttt tggcttccgt catcggcttt ttggtcgccg
     2701 ccgcctgcgg ttatatggcg ggtttggtcg gctcgtcttc cagcccgatt tccggcgtgg
     2761 gcatcgtttc catcgtcgtg atttcgctgg ttttgctgct ggtgggcgaa tccggcggtt
     2821 tgatggcgga tgaggcaaac cgcaaatttt tgctggcact gactttattt tgcggctcgg
     2881 cagtaatctg cgtggcttcg atctccaatg acaacctgca agacctgaaa accggctacc
     2941 tgctcaaagc cacgccttgg cggcagcaag tcgccctgat tatcggctgt atcgtcggtg
     3001 cgctggttat ttcacccgtg ttggaactgc tttacgaagc ctacggcttt accggcgcaa
     3061 tgccgcgcga aggtatggac gcggcgcagg ctttggcagc ccctcaagcg actttgatga
     3121 cgaccatcgc gtcgggcatt ttcgcccaca accttgaatg ggtctatatc tttaccggca
     3181 tcgcgattgg cgcggtgttg attgccgccg atttggtgtt gaaaaaatca tcaggcggca
     3241 aacttgccct gcccgtcctc gcggtcggta tgggcattta tctgccgccg tccgtcaata
     3301 tgcccatcgt ggcaggcgcg gtgttggcgg cggtgttgaa acacatcatc ggcaaaaaag
     3361 cggaaaaccg tgaaggccgt ctgaaaaacg ccgaccgtat cggtacgctt ttcgcggccg
     3421 gtctgattgt cggtgaaagt ctgattggtg tgattatggc gtttattatt gccttctccg
     3481 tgaccaacgg cggctcggac gcgccgctcg cgttgaatct gcaaaactgg gatgccgccg
     3541 cttcttggct gggcttggcg ttcttcgtta ccggaatgat tatttttgca cggcgcgtac
     3601 tgaaggcggg taggtagcct gtcggagaaa atgccgtctg aaacgttcag acggcatttt
     3661 ttatcgggaa agtgtaaggc ggagcttttc ggcttgcgcc cacgttttgc cggcaaggtc
     3721 tttgggcgac agcagcggcg cggtttgaag cggccagcct atgccgactg tcgggtcgtt
     3781 ccatattaaa acctgttcgg cttcaggctt gtaatagtcc gtgcatttgt acacgacttc
     3841 cgctgcgtcg cccaaaacgc aaaacccgtg cgcgaagcct tcgggtatcc aaagctggca
     3901 ttggttttgc gcggacaggg ttgcgcccgc ccatttgccg aaagtcggcg aaccttcgcg
     3961 catatcgatg gccacgtcga acacttcgcc gacaactacg cgtacgagtt tgccttgtgt
     4021 gttttcggtt tggtagtgca ggccgcgcaa tacgcctttg ccggatttgg agtggttttc
     4081 ctgcac
//
LOCUS       DAWFJR010000133         3967 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWFJR010000133 DAWFJR010000000
VERSION     DAWFJR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.133"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(114..770)
                     /locus_tag="WGR04_001974"
     CDS             complement(114..770)
                     /locus_tag="WGR04_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ0795457.1"
                     /translation="MKNKSKVWDFPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LFVLFLLVFRLCWGIWGSDTARFSRFVRGWSGIREYMKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKTALAAALSVAALVAAAILLLS
                     "
     gene            835..1725
                     /locus_tag="WGR04_001975"
     CDS             835..1725
                     /locus_tag="WGR04_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ0795458.1"
                     /translation="MFPVFHLSGESRRRMLQAASRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDALPFAVIAGFPEIMLDTADGLTKRQG
                     INAWMVGCGLGTGRAAVGTLAGILAEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIRAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGVWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITEST"
     gene            1843..3654
                     /gene="typA"
                     /locus_tag="WGR04_001976"
     CDS             1843..3654
                     /gene="typA"
                     /locus_tag="WGR04_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ0795459.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
BASE COUNT          946 a         1054 c         1165 g          802 t
ORIGIN      
        1 ggtttgggac ggaaaaacgg cctgaagccg tttcgggcat tcagaccgtt tgcgcggaga
       61 ggggatgccg tccgaagggc gaaaagggct tcagacggca ttgatgtcgg gtttcaggac
      121 aggagcagga tggcggctgc gacaagcgcg gcaaccgata atgcggcggc aagcgcggtt
      181 ttgccggcaa agcggattga ggttttgcct tcgatgtatt tgaagccggt tatcatcggg
      241 aggacgaggt ttttcttttt gaacacgcgg tatgcggcga cggcggcgat atgaatggcg
      301 gaaaaaacgg cgagcagctt gaaaaagttg aggtggattt tccgcataag gctgcccgta
      361 tgctcggaaa ccaaatggtt gaggtagccg ttggtgctga aggtgttttc atcggcggca
      421 aaaagccctg tgccgacttg gaacgacacg gcggccagaa gcgcgacgac catcagtgcg
      481 cccaaggggt tgtgtccggg ctggatgtgt tcgggaatac cgtttttcat atactctctg
      541 atacccgacc atccgcggac gaaacgggag aaacgggcgg tatcgctgcc ccaaatgccc
      601 cagcagaggc ggaatacgag caggaaaagg acgaacagcc cgacgcgcgt gtgccattgc
      661 agcatatcgc cgccggcttt cgcgctatac cacataaagg gcagggatgc ggcaagcagc
      721 cagtggaaaa ggcgggtggg gaagtcccag actttgcttt tgtttttcat aatcggtttc
      781 cggcggtaga atcggtttgt tttcgagcct taattttaac cgattggagg ggaaatgttt
      841 cccgttttcc atctttcagg cgaaagccgc cgccggatgc ttcaggcggc atcgcgtttt
      901 ccctatgttt tcaaagcccg tgcggaagat tcgcacaaag ggactttcgg cacgctcgcc
      961 gtagtcggcg gatcggcagg gatgagcggc gcgcccgtat tggcggcatc ggcggcgatg
     1021 tatctcggct gcggcaaggt gtgggcgggt ttcaatcagg atgcgcttcc gtttgccgtc
     1081 attgccggtt ttcccgaaat tatgttggac acggcagacg gtttgaccaa gcgtcaaggc
     1141 ataaacgctt ggatggtagg ttgcggcctg ggtacaggta gggcggcggt cggaacgctt
     1201 gccggaattt tggcggaaca cacggacaag cccgtccttt tggatgcgga cgcgctgaac
     1261 atattatcaa ccgatgccga aacccgaaat ctggcgcgcg ggtgtaaaaa cctgatttta
     1321 acgccacacc ccgccgaagc cgcgcgcctg cttggaacga cggttgcaca ggttcaggcg
     1381 gatcggacgg cggcagtgag gaagataagg gcaattttcg gcgcaaccgt ggttttaaag
     1441 gggcacaaaa cattggttgc ctcacccgat acggaaatct atgtcaacga aagcggcaac
     1501 gcgggattgg caacggcggg cagtggcgac gtattgggcg gcatcatcgg cagtctgctc
     1561 gcacagggcg tgccggtttt tgaagccgcc tgcgcgggcg tgtggctgca cggcgcggcg
     1621 gcggatgtca taaaagaatc ggcaggcatt gcggcggggc tgttggctgg cgaaatcgcc
     1681 ccggcggcaa ggtggctgcg taaccggata actgaaagta cgtaatgaag atgccgtctg
     1741 aaaggcaagg gcttcagacg gcatcttcat ttcccaaata ctgtccggta aagcgtggta
     1801 taatgcgcag ttatctctta cagtttttga aaaacattaa ttatgaaaca aatccgcaac
     1861 atcgccatca tcgcccacgt cgaccacggc aaaaccacat tggtcgacca actgctgcgt
     1921 caatccggca cattccgcgc caaccagcag gttgacgagc gcgtgatgga cagcaacgac
     1981 ctcgaaaaag aacgcggcat caccatcctc gccaaaaaca ccgccatcga ttacgaaggc
     2041 taccacatca atatcgtcga cacgccggga cacgccgact tcggcggcga agtagagcgc
     2101 gttttgggga tggtggactg cgtcgtcttg ttggtggacg cgcaggaagg cccgatgccg
     2161 caaacccgtt tcgtgaccaa aaaagccttg gctttggggc tgaaaccgat tgtcgtcatc
     2221 aacaaaatcg acaagccgtc cgctcgtccg agctgggtca tcgaccaaac tttcgagctg
     2281 ttcgacaact tgggcgcgac cgacgagcag ttggatttcc cgattgttta tgcttccggt
     2341 ctgtccggtt tcgccaaatt ggaagaaacc gacgagagca acgacatgcg tccgctgttc
     2401 gatactatct taaaatatac gcctgcaccg agcggcagcg cggacgaaac gctgcaactg
     2461 caaatttccc aactcgacta cgacaactac accggccgcc tcggtatcgg tcgtatcttg
     2521 aacggacgaa tcaaacccgg ccaaaccgtt gccgtcatga accacgatca gcaaatcgcc
     2581 caaggccgca tcaaccagct tttgggtttc aaaggtttgg aacgcgtgcc gcttgaagaa
     2641 gccgaagccg gcgacatcgt gattatttcc ggtatcgaag acatcggtat cggcgtaacc
     2701 atcaccgaca aagacaatcc caaaggccta ccgatgttga gcgtggacga accgacgctg
     2761 acgatggact ttatggtcaa caccagcccg ctggcgggta cggaaggcaa attcgtaacc
     2821 agccgccaaa tccgcgaccg cctgcaaaaa gaattgctga ccaacgtcgc cctgcgcgtg
     2881 gaagataccg ccgatgccga cgtgttccgc gtatccgggc gcggcgagct gcacctgacc
     2941 attttgctgg aaaacatgcg ccgcgaaggc tacgaactcg ccgtcggcaa accgcgcgtc
     3001 gtgtaccgcg acatcgacgg tcaaaaatgc gaaccgtatg aaaacctgac cgtggatgta
     3061 cccgacgaca accaaggcgc ggtaatggaa gaactcggcc gccgccgtgg cgaactgact
     3121 aatatggaaa gcgacggcaa cggacgcacc cgcctcgaat accatattcc agcgcgcggc
     3181 ttgatcggtt tccaaggcga atttatgacc ctgacgcgcg gggtcgggct gatgagccat
     3241 gtgttcgacg attacgcgcc cgtcaaaccc gatatgcccg gccgccacaa cggcgtgctg
     3301 gtgtcccaag agcagggcga ggcagtcgct tacgccttgt ggaatctgga agaccgcggt
     3361 cgtatgttcg tatcacccaa cgacaaaatc tacgaaggta tgattatcgg catccacagc
     3421 cgcgacaacg atttggtggt caacccgctc aaaggcaaaa aacttaccaa catccgcgcc
     3481 agcggcaccg acgaagccgt gcgcctgacc acgccgatca agctgacgct ggaaggtgcg
     3541 gttgagttta tcgacgatga cgaactcgtt gaaatcacgc cgcaatccat ccgtctgcgc
     3601 aagcgttact tgagcgaatt ggaacgccgc cgccacttta aaaagctgga ttgatgttta
     3661 ctggattaat gtttaaatga tacgcgatgc cgtctgaaaa atttcagacg gcatttttta
     3721 ttcggacggg ctttgcggct tcttgaagct gtttcagacg gcgtttttcc tacccaatca
     3781 agaaactgcc gccatttttc cagcggtata tcgccccgtc gcgtttcggt atcgggttcg
     3841 gcttcccggc agcatgtcga acaatgccgt ttgaaggaat acgccttttg agtccttaca
     3901 agcttaggaa gaggatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
     3961 tagtaca
//
LOCUS       DAWFJR010000134         3926 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWFJR010000134 DAWFJR010000000
VERSION     DAWFJR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.134"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..332)
                     /locus_tag="WGR04_001977"
     CDS             complement(<1..332)
                     /locus_tag="WGR04_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ0795460.1"
                     /translation="MFSLYREDSLISRLNRDGYLTSPSADFLELLSLCRDIHALTDGA
                     FDPTVQALWKLYADYFAARPDAETPPPQNSVKEALKRVGFGKVSFDDREIRFAEKGMG
                     LSLNGIAQG"
     gene            complement(313..594)
                     /locus_tag="WGR04_001978"
     CDS             complement(313..594)
                     /locus_tag="WGR04_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nosX protein required for nitrous oxide
                     reduction"
                     /protein_id="HEZ0795461.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNKDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRQAADLVNKVLAEVARLEKIVQPLP"
     gene            complement(603..1397)
                     /locus_tag="WGR04_001979"
     CDS             complement(603..1397)
                     /locus_tag="WGR04_001979"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ0795462.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIYELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            1781..3115
                     /gene="gdhA"
                     /locus_tag="WGR04_001980"
     CDS             1781..3115
                     /gene="gdhA"
                     /locus_tag="WGR04_001980"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ0795463.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            3257..3631
                     /locus_tag="WGR04_001981"
     CDS             3257..3631
                     /locus_tag="WGR04_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795464.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            3675..>3926
                     /locus_tag="WGR04_001982"
     CDS             3675..>3926
                     /locus_tag="WGR04_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HEZ0795465.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQ"
BASE COUNT         1069 a         1014 c          923 g          920 t
ORIGIN      
        1 ccttgcgcga tgccgttcaa ggacaaaccc ataccctttt cggcaaaacg gatttcacgg
       61 tcgtcaaacg agactttgcc gaagccgacc cgtttcaggg cttccttcac gctgttttga
      121 ggaggcggcg tttccgcatc gggacgggcg gcaaaataat cggcatacag tttccacaac
      181 gcctgcactg tcggatcgaa cgcgccgtcc gtcagcgcgt gaatatcgcg gcacaggctc
      241 aacagttcca aaaaatccgc cgacggcgaa gtcagataac cgtccctgtt caggcggctg
      301 atcaggctgt cttcacggta aaggctgaac aattttttcc aaacgcgcca cttccgccaa
      361 aaccttgttg accaaatccg ccgcctgtct gtcgtccaca ccgaacaggc gcagctccgc
      421 accggaaccc agcgcgacac ctttccagaa gaacacattt tcattgcgtt tttcatcttt
      481 attgcgtttt tcatcatcgg cggcaaaagg attcggcagg aaagaaaccg ccgcgcccgc
      541 cgccgcaacg gcggcaactg tcaaaaaacg cctgcgcccg aaatgcctgc ccataccgcc
      601 tcctaaaccg acactgccgc cttgatgtgc ggatgggggt cgtaaccttc caactcgaaa
      661 tcttcaaact tgaaggaaaa caaatctttg acttcaggat tgattttcat cactggcaag
      721 gcgcgcggtt cgcgttccaa ctgcaatgcg gcctgctcga aatggttgcg gtacaaatgc
      781 gcgtcgccaa acgtatggac aaactcgccc gcctccaatc cgcacacttg cgccatcatc
      841 atggtcaaca atgcgtagct ggcaatatta aacggcacac caaggaaaat atctgcacta
      901 cgctggtaaa gctggcagga cagtttgccg tcggcaacgt aaaactgaaa cagcgcgtgg
      961 cagggcggca aggccatttc gtcgaccaaa gccggattcc acgccgatac aatcaggcgg
     1021 cgcgagtcgg ggtttttctt aatttgttcc accacattgg cgatttggtc gatatgcctg
     1081 ccgtcgggcg cgggccagtt acgccactgg tagccgtaaa ccgggcctaa gtcgccgttt
     1141 tcgtccgccc actcgtccca aatggaaaca ttgttgtcct ttaggtattt gatattggta
     1201 tcgcctttga gaaaccaaag cagctcgtag ataatcgaac gcagatgcag ctttttggtc
     1261 gtcagcagcg gaaaaccttt gcccaagtca aaacgcatct gataaccgaa tacggagcgc
     1321 gtgcccgtac cggtgcggtc tgatttgtcc gtaccgttgt cgaggacgtg gcgcatcaag
     1381 tccaaatagg ctttcatagc agtctttcat caaattaaac ggcgcatatt gtaacatttc
     1441 cggataatgc ccaaaacacg gatacagaca ggcaggattg ttggcaattt cagtcctttt
     1501 ccacagtaaa acccggtggg aaaacaaaat taccttgatt ggaatcaaaa aatctagttt
     1561 aattacttag aataaaattt caataatatt tttatttacg aaattaattt atgattattg
     1621 attaattatc ggcaacaaac atcaaacatc gaaaacatgg aaaaaataat gtcaacaatt
     1681 tttgccaaat cgggcttggc atcagaaaaa aataggttta tattcccacc tacaaatttg
     1741 ttttcccatt agtacactat caaccaaaag gagtatccga atgactgacc tgaacaccct
     1801 gtttgccaac ctcaaacaac gcaatcccaa tcaggagccg ttccatcagg cggttgaaga
     1861 agtcttcatg agtctcgatc cgtttttggc aaaaaatccg aaatacaccc agcaaagcct
     1921 gctggaacgc atcgtcgaac ccgaacgcgt cgtcatgttc cgcgtaacct ggcaggacga
     1981 taaagggcaa gtccaagtca accggggcta ccgcgtgcaa atgagttccg ccatcggtcc
     2041 ttacaaaggc ggcctgcgct tccatccgac cgtcgatttg ggcgtattga aattcctcgc
     2101 ttttgaacaa gtgttcaaaa acgccttgac caccctgcct atgggcggcg gcaaaggcgg
     2161 ttccgacttc gaccccaaag gcaaatccga tgccgaagta atgcgcttct gccaagcctt
     2221 tatgaccgaa ctctaccgcc acatcggcgc ggacaccgac gttccggccg gcgacatcgg
     2281 cgtaggcgga cgcgaaatcg gctatctgtt cggacaatac aaaaaaatcc gcaacgagtt
     2341 ttcttccgtc ctgaccggca aaggtttgga atggggcggc agcctcatcc gtcccgaagc
     2401 gaccggctac ggctgcgtct atttcgccca agcgatgctg caaacccgca acgatagttt
     2461 tgaaggcaaa cgcgtcctga tttccggttc cggcaacgtg gcgcaatacg ccgccgaaaa
     2521 agccatccaa ctgggcgcga aagtgctgac cgtttccgac tccaacggct tcgtcctctt
     2581 ccccgacagc ggtatgaccg aagcgcaact cgccgccttg atcgaattga aagaagtccg
     2641 ccgcgaacgc gttgccacct acgccaaaga gcaaggtctg caatactttg aaaaccaaaa
     2701 accgtggggc gtcgccgccg aaatcgccct gccctgcgcg acccagaacg aattggacga
     2761 agaagccgcc aaaaccctgt tggcaaacgg ctgctacgtc gttgccgaag gtgcgaatat
     2821 gccgtcgact ttgggcgcgg tcgagcaatt tatcaaagcc ggcatcctct acgccccggg
     2881 aaaagcctcc aatgccggcg gcgtagcaac ttcaggtttg gaaatgagcc aaaacgccat
     2941 ccgcctgtct tggactcgtg aagaagtcga ccaacgcctg ttcggcatca tgcaaagcat
     3001 ccacgaatcc tgtctgaaat acggcaaagt cggcgacaca gtaaactacg tcaatggtgc
     3061 gaacattgcc ggtttcgtca aagttgccga tgcgatgctg gcgcaaggct tctaagcaaa
     3121 cgccgccgct ccgcaaacaa aatgccgtcc gaaccgcaaa tgctgttcag acggcatttc
     3181 attatccgcc cgttcgaatc gggtgatact atttgtatcc agaattgttg gatagtttgg
     3241 gagtggagag gaagaaatga ttatggacca atactatatg gagttaaaga ataagttatc
     3301 taacagacca atcctactgg ataatactaa tgattttctt tttgttttag taaatacagt
     3361 gaaagctatg atagaaaata ctgataaaag tcagctttca gagttagata aaattttaga
     3421 cggtgtcaca agtcaagaat tgaaacttgc ctatgatttt tgtcaaggga aattcggtca
     3481 agcaggtttt tcttatcgta ggcacccgaa ttatttttat ttgtccagtt taattgcgac
     3541 ttttccagaa tttgagttgt ctaaagcaga ccgagattac ctcaaaggaa tcataaattt
     3601 tgataactac cttttatatg agttagatta gtctttaatc acctcaaaac cctatttatt
     3661 caaaaggaga aaaaatgatt gagctgaaat tagtagatga gagcagtttt caggcagtgc
     3721 tggatttgaa aatatctgaa gctgatgaac gagcacgttt tgtggctcca aatgtacgct
     3781 ctttagctga cgcatggctc taccgggaaa atgaagatgt gtttccgagg gcaatctatt
     3841 gggataagca agtggttggc tttctcctgc tggaaataga caaggatgaa gcagaatact
     3901 ttatctggcg gataatgatt ggtcag
//
LOCUS       DAWFJR010000135         3872 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWFJR010000135 DAWFJR010000000
VERSION     DAWFJR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.135"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="WGR04_001983"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="WGR04_001983"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ0795466.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="WGR04_001984"
     CDS             1439..1924
                     /locus_tag="WGR04_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ0795467.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WGR04_001985"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WGR04_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ0795468.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVGTLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGTKPKRIKYKPVTR"
BASE COUNT          788 a          941 c         1083 g         1060 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cttcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 ccgtcagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataag
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgccga tgtcaaacct
      661 gaggacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggcgcg
      721 ataccgcgtt gggtgttgtt aagtgcggct ttgcccgaat tggatgaggt atggctggaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcattgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgt ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgtgcctt cttcgccgag
     2041 tcggcgtttc ttgtcggctt cgtattcctg atagttgccg tcaaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccagc ggtcgtgcga
     2161 aatcaccatc acgctgccgg caaattccag caatgcgtct tccaacgcgc gcagggttcc
     2221 cacgtcgagg tcgttggacg gttcgtccag cagcaacaca ttgccaccgc ccaacaaggt
     2281 ttttgccaag tgcaaacgtc cgcgttcgcc gccggaaagc tgccccgtga ttttgctttg
     2341 gtcgctgcct ttgaaattga agcgtcccaa atattggcgg gcggggattt caaactgccc
     2401 gacctgtaaa atatcgcgac cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgcccgattt tcacttcacc
     2521 ggaatcgggc tgctctttgc ccgcaatcat tttaaacagt gtcgatttac ccgcgccgtt
     2581 cggaccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttgtcg ccgaacgatt tggaaacatt cacaaattca atcacttcgt tacccaaacg
     2701 ctcggcgacg ggaatgaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgtgttacc gcaacgactg taccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 agattccgcg tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gtttgctcaa
     3121 caagagtttg cacaaggcaa cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc ggcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacctgccg caataatcgc ttccaagcgg ccctgttctt ccgccaacgc
     3301 gtcaaaatcc gcatcaggat tggcgtactc ggcatacact tcttccaaac gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcgcgc acggtttttt caggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt aatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacaccag ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcaaa ccgattttcg cgccggggaa gaaggaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 agttttctcg tttcggtcgg acaaatttca gacggcacat tttaaccgat aatttgattt
     3721 aagccagttt atccgcgaac cggtattgcc aaaatcgggc aggattcata aaatccgtct
     3781 atccctttga aattatacaa acaaaaaaaa taataatgat aggtgatcgc cgcctcggca
     3841 accattttgg attttccaaa gcaaatatag tg
//
LOCUS       DAWFJR010000136         3844 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWFJR010000136 DAWFJR010000000
VERSION     DAWFJR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.136"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            330..3533
                     /gene="nalP"
                     /locus_tag="WGR04_001986"
     CDS             330..3533
                     /gene="nalP"
                     /locus_tag="WGR04_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HEZ0795469.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGTSAPDFNAGG
                     TGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPNLHT
                     GDFTNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYNE
                     NYKNYTAYMRKEAPEDGGGKDIKASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHIIG
                     GRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFGTT
                     SRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNMLFI
                     FSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLSAP
                     YEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIGAV
                     GVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKGGS
                     QLQLHGNNTYTGKTIIEGGSLMLYGNNKSDMRVETKGALIYNGAASGGSLNSDGIVYL
                     ADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSARGKGAGYL
                     NRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRGNA
                     ARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMPGI
                     RPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLDHN
                     ATGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGHSTWSE
                     NSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGTLM
                     QLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGLAG
                     LKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVAGL
                     GADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1038 a         1044 c         1015 g          747 t
ORIGIN      
        1 taaagaatcg ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttcatcca
       61 ctataactag ggaaattcaa attaagttag aattatccct atgagaaaaa gccgtctaag
      121 ccggtataaa caaaataaac tcattgagct atttgtcgaa agttcaaatt tccattttaa
      181 aacaattagt aaaatcgagt ttatcctagt tgtccaagac aacccctata ataatataat
      241 tcaaaatata aaaatgggtt acatctaaac attacggaat ttttattccc tcgcctgaat
      301 tctattgtca gattcaagga gacctcatca tgcgaacgac cccaaccttc cctacaaaaa
      361 ctttcaaacc ggctgccatg gcgttagctg ttgcaacaac actttctgcc tgcttaggcg
      421 gcggcggcgg cacttctgcg cccgacttca atgcaggcgg caccggtatc ggcagcaaca
      481 gcagggcaac aacagcgaaa tcagcagcag tatcttacgc cggtatcaag aacgaaatgt
      541 gcaaagacag aagcatgctc tgtgccggtc gggatgacgt tgcggttaca gacagggatg
      601 ccaaaatcaa tgcccccccc ccgaatctgc ataccggaga ctttacaaac ccaaatgacg
      661 catacaagaa tttgatcaac ctcaaacctg caattgaagc aggctataca ggacgcgggg
      721 tagaggtagg tatcgtcgat acaggcgaat ccgtcggcag catatccttt cccgaactgt
      781 atggcagaaa agaacacggc tataacgaaa attacaaaaa ctatacggcg tatatgcgga
      841 aggaagcgcc tgaagacgga ggcggtaaag acattaaagc ttctttcgac gatgaggccg
      901 ttatagagac tgaagcaaag ccgacggata tccgccacgt aaaagaaatc ggacacatcg
      961 atgtggtctc ccatattatt ggcgggcgtt ccgtggacgg cagacctgca ggcggtattg
     1021 cgcccgatgc gacgctacac ataatgaata cgcatgatgg aaccaagaac gaaataatgt
     1081 ctgcagccat ccgcaatgca tgggtcaagc tgggcgaacg tggcgtgcgc atcgtcaata
     1141 acagttttgg aacaacatcg agggcaggca ctgccgacca tttccaaata gccaattcgg
     1201 aggagcagta ccgccaagcg ttgctcgcct attccggcgg tgataaaaca gacgagggta
     1261 tccgcctgat gcaacagagc gattacggca acttgtccta ccacatccgt aataaaaaca
     1321 tgcttttcat tttttcggca agcaatgacg cacaagctca gcccaacaca ctgaccctat
     1381 tgccatttta tgaaaaagat gctcaaaaag gcattatcac agtcgcaggc gtagaccgca
     1441 gtggagaaaa gttcaatggc tccaaccatt gcggaattac tgccatgtgg tgcctatcgg
     1501 caccctatga agcaagcgtc cgtttcaccc gtacaaaccc gattcaaatt gccggaacat
     1561 ccttttccgc acccatcgta accggcacgg cggctctgct gctgcagaaa tacccgtgga
     1621 tgagcaacga caacctgcgt accacgctgc tgacaacggc tcaggacatc ggtgcagtcg
     1681 gcgtggacag caagttcggc tggggactgc tggatgcggg taaggccatg aacggacccg
     1741 cgtcctttcc gttcggcgac tttaccgccg atacgaaagg tacatccgat attgcctact
     1801 ccttccgtaa cgacatttca ggcacgggcg gcctgatcaa aaaaggcggc agccaactgc
     1861 aactgcacgg caacaacacc tatacgggca aaaccattat cgaaggcggt tcgctgatgt
     1921 tgtacggcaa caacaaatcg gatatgcgcg tcgaaaccaa aggtgcgctg atttataacg
     1981 gggcggcatc cggcggtagc ctgaacagcg acggcattgt ctatctggca gataccgacc
     2041 gatccggcgc aaacgaaacc gtgcacatca aaggcgatct gcagctgggc ggcgaaggta
     2101 cgctgtacac acgtttgggc aaactgctga aagtggacgg tacggcgatg accggcggca
     2161 agctgtacat gtcggcacgc ggcaaagggg caggctatct caaccgtacc ggacaacgtg
     2221 ttcccttcct gagtgccgcc aaaatcgggc gggattattc tttcttcaca aacatcgaaa
     2281 ccgacggtgg tctgctggct tccctcgaca gcgtcgaaaa aacagcgggc agtgaaggcg
     2341 acacgctgtc ctattatgtc cgtcgcggca atgcggcacg gactgcttcg gcagcggcac
     2401 attccgcgcc cgccggtctg aaacacgccg tagaacaggg cggcagcaat ctggaaaacc
     2461 tgatggtcga actggatgcc tccgaatcat ccgcaacacc cgagacggtt gaaactgcgg
     2521 ccgccgaccg cacagatatg ccgggcatcc gcccctacgg cgcaactttc cgcgcagcgg
     2581 cagccgtaca gcatgcgaat gccgccgacg gtgtacgcat cttcaacagt ctcgccgcta
     2641 ccgtctatgc cgacagtacc gccgcccatg ccgatatgca gggacgccgg ctgaaagccg
     2701 tatcggacgg gttggaccac aacgctacgg gtctgcgcgt catcgcgcaa acccaacagg
     2761 acggtggaac gtgggaacag ggcggtgttg aaggcaaaat gcgcggcagt acccaaaccg
     2821 tcggcattgc cgcgaaaacc ggcgaaaata cgacagcagc cgccacactg ggcatgggac
     2881 acagcacatg gagcgaaaac agtgcaaatg caaaaaccga cagcattagt ctgtttgcag
     2941 gcatacggca cgatgcgggc gatatcggct atctcaaagg cctgttctcc tacggacgct
     3001 acaaaaacag catcagccgc agcaccggtg cggacgaaca tgcggaaggc agcgtcaacg
     3061 gcacgctgat gcagctgggc gcactgggcg gtgtcaacgt tccgtttgcc gcaacgggag
     3121 atttgacggt cgaaggcggt ctgcgctacg acctgctcaa acaggatgca ttcgccgaaa
     3181 aaggcagtgc tttgggctgg agcggcaaca gcctcactga aggcacactg gtcggactcg
     3241 cgggtctgaa gctgtcgcaa cccttgagcg ataaagccgt cctgtttgca acggcgggcg
     3301 tggaacgcga cctgaacgga cgcgactaca cggtaacggg cggctttacc ggcgcgactg
     3361 cagcaaccgg caagacgggg gcacgcaata tgccgcacac ccgcctggtt gccggtctgg
     3421 gcgcggatgt cgaattcggc aacggctgga acggcttggc acgttacagc tacgccggtt
     3481 ccaaacagta cggcaaccac agcggacgag tcggcgtagg ctaccggttc tgacggacag
     3541 gaagcagaca gccgcaaaga tcacggtctt tgcggctgtt tcttatgaaa agaaaaccct
     3601 attccaattg cctgcttcta ttgtttcaag acttcttcca aagattcggc atcaatcaga
     3661 tgtatagcgg attaacaaaa atcaggacaa ggcggcgaag ccgcagacag tacagaaccg
     3721 attcactcgg tgcttcagca ccttagagaa tcgttctctt tgagctaagg cgaggcaacg
     3781 ctgtactggt ttttgttaat ccgctatatt ccaccatctc taagatttac agcgatacac
     3841 aggt
//
LOCUS       DAWFJR010000137         3813 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWFJR010000137 DAWFJR010000000
VERSION     DAWFJR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.137"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            152..2917
                     /locus_tag="WGR04_001987"
     CDS             152..2917
                     /locus_tag="WGR04_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0795470.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQEGGLKFNSDSGKWER
                     DFQRPYWNTIYYKQYNDPQKLQKYIEEHDKSWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYGSDDEFKRAFGENSPTYKKHCNPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFAGYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGNIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNFRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADGLCNGKYGGTSKSVLTNFARGRTFL
                     MTMSYKF"
     gene            2947..3189
                     /locus_tag="WGR04_001988"
     CDS             2947..3189
                     /locus_tag="WGR04_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795471.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESRHSMLRQFIEND"
BASE COUNT         1052 a          941 c          915 g          905 t
ORIGIN      
        1 acaacgctgt accggttttt gttaatccac tataaatatc caattgaaat cttcagacgg
       61 tatatcgaat ttacactttt taatgtttat gccgcctaaa aaaatgctaa tatatttctt
      121 aattgtctga ctgtttattg ttgaggaaaa tatgagatct tctttccggt tgaagccgat
      181 ttgtttttat cttatgggtg ttatgctata tcatcatagt tatgccgaag atgcagggcg
      241 cgcgggcagc gaggcgcaga tacaggtttt ggaagatgtg cacgtcaagg cgaagcgcgt
      301 accgaaagac aaaaaagtgt ttaccgatgc gcgtgccgta tcgacccgtc aggatatatt
      361 caaatccagc gaaaacctcg acaacatcgt acgcagcata cccggtgcgt ttacacagca
      421 agataaaagc tcgggcattg tgtctttgaa tattcgcggc gacagcgggt tcgggcgggt
      481 caatacgatg gtggacggca tcacgcagac cttttattcg acttctaccg atgcgggcag
      541 ggcaggcggt tcatctcaat tcggtgcatc tgtcgacagc aattttattg ccggactgga
      601 tgtcgtcaaa ggcagcttca gcggctcggc aggcatcaac agccttgccg gttcggcgaa
      661 tctgcggact ttaggcgtgg atgacgtcgt tcagggcaat aatacctacg gcctgctgct
      721 aaaaggtctg accggcacca attcaaccaa aggtaatgcg atggcggcga taggtgcgcg
      781 caaatggctg gaaagcggag catctgtcgg tgtgctttac gggcacagca ggcgcagcgt
      841 ggcacaaaat taccgcgtgg gcggcggcgg gcagcacatc ggaaattttg gcgcggaata
      901 tctggaacgg cgcaagcagc gatattttgt acaagaaggc gggttgaaat tcaattccga
      961 cagcggaaag tgggagcggg atttccaaag gccgtactgg aataccattt actataaaca
     1021 atacaatgac ccccaaaaac tgcaaaaata catcgaagag catgacaaaa gctggcggga
     1081 aaacctggca ccgcaatacg acattacccc catcgatccg tccagcctga agcagcagtc
     1141 ggcaggcaat ctgtttaaat tggaatacga cggcgtattc aataaataca cggcgcaatt
     1201 tcgcgattta aacaccaaaa tcggcagccg caaaatcatc aaccgcaatt atcaattcaa
     1261 ttacggtttg tctttgaacc cgtataccaa cctcaatctg accgcagcct acaattcggg
     1321 caggcagaaa tatccgaaag ggtcgaagtt tacaggctgg ggacttttaa aggattttga
     1381 aacctacaac aacgcaaaaa tcctcgacct caacaacacc gccaccttcc ggctgccccg
     1441 cgaaaccgag ttgcaaacca ctttgggctt caattatttc cacaacgaat acggcaaaaa
     1501 ccgctttcct gaagaattgg ggctgttttt cgacggtccg gatcaggaca acgggcttta
     1561 ttcctatttg gggcggttta agggcgataa agggctgctg ccccaaaaat caaccatcgt
     1621 ccaaccggcc ggcagccaat atttcaacac gttctacttc gatgctgcgc tcaaaaaaga
     1681 catttaccgc ttaaactaca gcaccaatac cgtcggctac cgtttcggcg gcgaatatac
     1741 gggctattac ggctcggatg acgaatttaa gcgggcattc ggagaaaact cgccgacata
     1801 caagaaacat tgcaacccga gttgcgggct ttatgaaccc gtattgaaaa aatacggcaa
     1861 aaagcgcgcc aacaaccatt cggtcagcat tagtgcggac ttcggcgatt atttcatgcc
     1921 gttcgccggc tattcgcgca cacaccgtat gcccaacatc caagaaatgt atttttccca
     1981 aatcggcgac tccggcgttc acaccgcctt aaaaccagag cgcgcaaaca cttggcaatt
     2041 tggcttcaat acctataaaa aaggattgtt aaaacaagat gatacattag gattaaaact
     2101 ggtcggctac cgcagccgca tcgacaacta catccacaac gtttacggga aatggtggga
     2161 tttgaacggg aatattccga gctgggtcag cagcaccggg cttgcctaca ccatccaaca
     2221 ccgcaatttc aaagacaaag tacacaaaca cggttttgag ttggagctga attacgatta
     2281 tgggcgtttt ttcaccaacc tttcttacgc ctatcaaaaa agcacgcaac cgaccaactt
     2341 cagcgatgcg agcgaatcgc ccaacaatgc gtccaaagaa gaccaactca aacaaggtta
     2401 tgggttgagc agggtttccg ccctgccgcg agattacgga cgtttggaag tcggtacgcg
     2461 ctggttgggc aacaaactga ctttgggcgg cgcgatgcgc tatttcggca agagcatccg
     2521 cgcgacggca gaagaacgct atatcgacgg caccaacggg ggaaatacca gcaatttccg
     2581 gcaactgggc aagcgttcca tcaaacaaac cgaaactctt gcccgccagc ctttgatttt
     2641 tgatttttac gccgcttacg agccgaagaa aaaccttatt ttccgcgccg aagtcaaaaa
     2701 tctgttcgac aggcgttata tcgatccgct cgatgcgggc aatgatgcgg caacgcagcg
     2761 ttattacagc tcgttcgacc cgaaagacaa ggacgaagac gtaacgtgta atgctgatgg
     2821 cttgtgcaac ggcaaatacg gcggcacaag caaaagcgta ttgaccaatt ttgcacgcgg
     2881 acgcaccttt ttgatgacga tgagctacaa gttttaaagg cagcccgcgt tttgtagaaa
     2941 accgcaatgc cgtctgaaag cccttcagac ggcatttgtt tccccaaacg catcatcctg
     3001 ccgcaagcct atgccaatcc gttttatcgc atcggcaact caaagaaaaa tccatttcat
     3061 tcccacgcag ggaagccggt ttttgatttc ggttattttt ggttgtttcg ggtaatttat
     3121 gagtcgtcat tcccgcaaaa gcgggaatct agacattcaa tgctaaggca atttatcgaa
     3181 aatgactgaa actcaaaaaa ctggattccc actttcgtgg gaatgacgcg gtgcaggttt
     3241 ccgtatggat ggattcgtca ttcccgcgca ggcgggaatc tagacattca acgctaaggc
     3301 aatttatcgg aaatgactga aactcaaaaa actggattcc cactttcgtg ggaatgacgt
     3361 ggtgcaggtt tccgtatgga tggattcgtc attcccccgc aggcgggaat ctagtccgtt
     3421 cggtttcggt ttttttggct aatgccgcaa cattaaattt ctagattccc actttcgtgg
     3481 gaatgacggc ggagcggttg ctgtttttcc caataaatgc cccccaacct aaaatccgtc
     3541 attcccgcgc aggcgggaat ctagtccgtt cggtttcggt ttttttggct agtgccgcaa
     3601 cattaaattt ctagattccc actttcgtgg gaatgacggc ggatcggttt ctgctttttc
     3661 caataaatga ccccaaccta aaatccgtca ttcccgcaca ggcgggaatc tagacattca
     3721 atgctaaggc aatttatcgg gaatgactga aactcaaaaa actagattcc cactttcgtg
     3781 ggaatgacgc ggtgcaggtt tccgtacgga tag
//
LOCUS       DAWFJR010000138         3757 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWFJR010000138 DAWFJR010000000
VERSION     DAWFJR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.138"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            1..838
                     /locus_tag="WGR04_001989"
                     /pseudo
     CDS             1..838
                     /locus_tag="WGR04_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1238..1570
                     /locus_tag="WGR04_001990"
                     /pseudo
     CDS             1238..1570
                     /locus_tag="WGR04_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
     gene            complement(1860..2654)
                     /locus_tag="WGR04_001991"
     CDS             complement(1860..2654)
                     /locus_tag="WGR04_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ0795472.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDSPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            2698..2802
                     /locus_tag="WGR04_001992"
                     /pseudo
     CDS             2698..2802
                     /locus_tag="WGR04_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            2863..3588
                     /locus_tag="WGR04_001993"
     CDS             2863..3588
                     /locus_tag="WGR04_001993"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ0795473.1"
                     /translation="MPRFYLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAAQLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLADIRQPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT         1016 a         1134 c          856 g          751 t
ORIGIN      
        1 tgttgtccat gttcaaagcc gtcctgctcg gacaatggca cagcctctcc gatcccgaac
       61 tcgaacacag cctcatcacc cgcatcgatt tcaacctgtt ttgccgtttt gacgaactga
      121 gcatccctga ttacagcacc ttatgccgct accgcaaccg gcggcgcaag acgacaccct
      181 atccgaattg ctcaaactga tcaaccgcca actgaccgaa aaaggcctaa aaatagagaa
      241 agcatccgcc gccgttgttg acgccaccat tattcagacc gccggcagca cacagcgtca
      301 ggctatagaa gtcgatgaag aaggacaaat caacggccaa accacaccga gtaaggacaa
      361 agatgcccgt tggatcaaga aaaacggcct ctacaaactc ggttacaaac aacatacccg
      421 taccgatgcg gaaggctata tcgagaaact gcacatcacc cccgccaatg cccatgagtg
      481 caaacacctg tcgccgttgt tagaagggat agctgaaagt acgaccgtct atgccgacaa
      541 aggctatgac agtgcggaaa accggcaaca tctgaaagaa catcagttgt tggacggcat
      601 tatgcgcaaa gcctgccgca accgtccgct gacggaagcg caaaccaaac gcaaccgata
      661 tttgtcgaag acccgttatg tggtcgaaca aagcttcggt acgctgcacc gcaaattccg
      721 ctatgcccgg gcagcctatt tcggactgat taaagtgagt gcgcaaagcc acctgaaggc
      781 gatgtgtttg aacctgttga aagccgccaa caggctaagt gcgcccgctg ccgcctaaaa
      841 ggcagcctgg atgcctgatt atcgggtatc cggggaggat taagggggta tttgggtaga
      901 attaggagta attaggagcg aaaatagacg aaaacctgtg tttgggtttc ggctgtcggg
      961 agggaaagga attttgcaaa ggtctcgggc tatgccggct gctgccgccc attgcaccct
     1021 cgccgaatcc tgccgcccac cgccgaagcc ctcatgcgtt cgcggtacag cgcgtatgtg
     1081 ctactatagt ggattaacaa aaaccagtac agcgttgcct cgccttagct caaagagaac
     1141 gattctctaa ggtgctgaag caccgagtga atcggttccg tactatttgt actgtcttcg
     1201 gcttcgtcgc cttgtcctga tttttgttaa tccactatac atcatcgcta ctaccgttcc
     1261 ggcgcaacaa acattcctcg atgccgccga actgatgcaa tggagtatag aaaccgaagg
     1321 gctgggcttg aacgtcatct cgcacaagat actcggcaaa gaccacgccc aagtcgaatt
     1381 tgaagcctac ttccgagacg gacaacaccg atccgcgcat cacgaactgt ccggcttcat
     1441 caacatcggc ggacaatggt attttatcga tcctaccgtt ccgcatcctg cgatgaaaca
     1501 accctgcatt tgcggatcgg gcaaaaaatt caaagcctgc tgcggcaaat atctgcaacc
     1561 tgtcgcataa aaatgccgtc tgaacgttca gacggcattt ttaacgcgca aaaaaaccat
     1621 tcataccaag ggtaagtatg aatggtcaat acattgcggg aaaacgtctt acttgctgca
     1681 ctgccgaaaa gggagaaacg gcagcggtaa tcagcggaaa ggattgtacc cgtataaatg
     1741 ttaagtaacg ttaattatga aaatataacc gatgtttcgg cacacaatcc gcaactgtcc
     1801 ggcgcaggcg gcggcaaagc ctgtttattc aaacccttat tcctgcccat ctgtccacct
     1861 tactggtttt cccgtatcca cccgacccag cgttcaaaca gcttcggttc gagcgcggca
     1921 acgaccgagc gtttgaacac gtgttcacca ctccaaaacc cgtcgccttc caaagtcgtc
     1981 agcctgccgc ccgcctcctc gaaaatcaac gcacccgcag catagtccca cagcttctgc
     2041 ccaccgtgaa cataaacatc ataacgcccg cacgccagat aacaccaatc caacgtactg
     2101 ctgcccatac tccgtatcgt tccaaaaggc gcgagcgtac tcatacggct ggaaagtttg
     2161 cccgaacgca aatatttgat ttccacgccc gcaatcgcct cattgagttt tttatccacg
     2221 agacgcaaag gcaggcgggt tccattaaga aacgcaccct gcccccgttc ggcataaaaa
     2281 cattcgccgc tgaccggatt gtaaatcaca cccaattcgg cgcgcccgtt gcggacaaac
     2341 gccaccgaca cggcaaaatg cggcagcccg ttgacaaaat tgttcgtccc gtctatcgga
     2401 tcgacaatcc acagcccctt ttcccccgaa tattgttccc acaaagccga ctgttcctgc
     2461 cgcgacattt cctcgcccaa catcggactg tcgattaaaa gcggcaacgc ggcggcaaaa
     2521 gccgtctgcg cggcaatgtc cgcctcgctc aacatcgaac cgtcttcctt gcggtgagac
     2581 ggcgtattca aaaaacgcgg cataatttcg gtttgcgcga tatggcgcac gactttctgc
     2641 aaacggtgta acacttccta ctgtcctcat attttgaact tgcggcacgc gaacgtatat
     2701 agtggattaa atttaaatca ggacaaggcg acgaagccgc agacagtaca aatagtacgg
     2761 caaggcgagg caacgctgta ctggtttaaa tttaatccac tataatgtcc gtttccatca
     2821 cgccgctgcg acagattata accgtccgaa ccgccaaaaa ctatgccccg attctacctg
     2881 cccgaaaacc tttccgtcgg acaaaccgtc gccctgcccg acaacatcgt ccgccacctc
     2941 aacgtcctac gcgtccgccc caacgaaaac atcaccctct tcgacggcaa aggcaaggca
     3001 cacgccgcac agctgaccgt tttggaaaaa cgccgcgccg aagccgaaat cctgcacgaa
     3061 gacacaaccg acaacgagtc cccgctcaac atcacactga tacaatccat ctcctccggc
     3121 gatcgcatgg atttcaccct gcaaaaaagc gtcgaactcg gcgtaaccgc catacagccc
     3181 gtcatcagcg aacgctgcat cgtccgcctc gacggagaac gcgccgccaa acgcctcgca
     3241 cgctggcagg aaatcgtcat ctccgcgtgc gaacaaagcg gcaggaacac cgttcccccc
     3301 gtactgccca tcatcggcta ccgtgaagca ctcgacaaaa tgccgtctga aagcaccaag
     3361 ctgattatga gcatcaaccg cgcccgcaaa cttgccgaca tacgccaacc gtccggcgca
     3421 atcgtcttta tggtcgggcc agaaggcggc tggacagaac aggaagaaca acaggcattt
     3481 gaagctggct ttcaggcggt tacactcggc aaacggattt tacgcacaga aaccgcccca
     3541 ctcgccgccc tcgccgccat gcagacgctt tggggcgatt tcgcataaac agaaatgccg
     3601 tctgaaaccc gttcagacgg cattttgcag ccgattaaga tagtaggttc aaataagatt
     3661 tcccgtgtcg tcattcccgc gaaagcggga atctagaaac gaaaaactac agggatttat
     3721 ccgaaacaac aaccctctcc gccgtcattc ccgcgca
//
LOCUS       DAWFJR010000139         3745 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWFJR010000139 DAWFJR010000000
VERSION     DAWFJR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.139"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(88..906)
                     /locus_tag="WGR04_001994"
     CDS             complement(88..906)
                     /locus_tag="WGR04_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0795474.1"
                     /translation="MKGMNKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDRPDPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIGNPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FAPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDIFRNFSL"
     gene            complement(986..2236)
                     /locus_tag="WGR04_001995"
     CDS             complement(986..2236)
                     /locus_tag="WGR04_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0795475.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAVRIWPS
                     HTYTRLTLESTAALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPDTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDADDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYLNTSVLKRG"
     gene            complement(2233..2694)
                     /gene="tsaE"
                     /locus_tag="WGR04_001996"
     CDS             complement(2233..2694)
                     /gene="tsaE"
                     /locus_tag="WGR04_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ0795476.1"
                     /translation="MSDLPSISRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYLLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGDGRKCLLTAHTERGRESLPL"
     gene            2896..3708
                     /locus_tag="WGR04_001997"
     CDS             2896..3708
                     /locus_tag="WGR04_001997"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ0795477.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRNNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT          839 a          906 c         1111 g          889 t
ORIGIN      
        1 cactataacc gaaggtaagg gaggggtgtg tccactgtgc cgccgcattt gccgcgtgcg
       61 gaagatacgg ataacaaccc cttggaatta aagggagaag ttgcggaaaa tatccatatc
      121 aggctgtggg ccgtacttat ttacggtatt ttcgtatgcc gaaataaaat atttcttgaa
      181 taaaccgtaa tcgccggttt cgtaataggc tacgacagcc tcggcatagg cgggataact
      241 gtcgggggcg aatacgcaag gaaaaaaacc ggagcgcatc agcgacaagg tcatacagtt
      301 tctggcagta cgtttattgc aatctttaaa atattgaaga tacgccaaat tattatggag
      361 ataaacggcg cggtcgaagg gatttcctat ttttggggct tcttgaagca accattttaa
      421 ttccgtatcc aaactttgcg ggttggataa aggcgtgtag tctgtcccgc ttatagtaac
      481 gctgtcgcgg cggactacgc cgccggagcc tttttccagt aagttttccg aaatcaggct
      541 gtgggtggtt tttaagaaat ccaaccagtc aaacggatcg gggcggtcca aaccggacag
      601 caggtgccgg taactttccc tcaggttgat taacattacg gcatccgaat ataacttccc
      661 gcctgcggtt tgcccgagtt taagcaatgc ctgcgtatcg tattgattat aagtgtttcc
      721 ttccaatttg gcagaactgt acacaaaatc gataccgatt tcttcaatat tacgcacgga
      781 gtccgttaaa acagataaag gaagccgatc ggacaattcg gtcaggtatt cttgttcgcc
      841 ggctgtaaaa atggggcgga tatttaaaaa atcctgttgg tatctcaatt tattcatacc
      901 tttcatcata aaaacgcggt ttggggcgga agtaggttta tgcctgcctt catccgacaa
      961 ctatattgaa acaccgcctt ttcaatcaac cccgcttcaa tacggatgta ttgaggtagc
     1021 gttggacacc cgaggcaatg gattgggcgc actgccgacg gaaggattcg ctgcccagca
     1081 gcttctcttc ggcaggattg gacaggaagg cggtttcgac caggatagac ggcatatcgg
     1141 gcgcgcgcaa aacggcgaaa ttggcttcgt ccaccctgcc tttgtgcaag tggttgagcc
     1201 tgcccaattc ttcaagcacc agtttgccga gtttgcggct gtcgcgcagc gtggcggttt
     1261 gggtcatgtc gagcagggcg gtatcgacat tgcggttgcc gctggtcggt acgccgccga
     1321 ccgcgtcggc attattttgg gtttgttcca agaatttggc ggcagagctg gtcgcgcctt
     1381 tggtgttcag catataaacc cccgttccgc gcgcggaggg gctggtgaaa gcgtcggcgt
     1441 ggatggagac gaatacgtcc gcccgccgtg cgcgcccttt ggcgacacgc acgcccaatg
     1501 ggatgaacac gtcttcgttg cgcgtcataa agacgttgta acccaaggct tccaattgtt
     1561 ttttggtttc gcgggcgatg gagaggacga cgtgtttttc ctgcaaaccg cccgggctga
     1621 cggcgccggg gtcttcgccg ccgtgtccgg ggtcgatcat aatgacgggt ctgcgcccgt
     1681 ttttgccgcg ttcggtttgt gcgggagtgt tttgagcgag gtcggcttca ggggagccgc
     1741 gacgggtttt gttcaggctg ccgttgagca gcgccatcat cggatcgtcg gcatccatcc
     1801 cttgcggata gagatcgacg acgaggcggt ttttaaagcc gccgacgggt gggagggaaa
     1861 aaacttgcgc gtaggaaggc tgtttcaaat cgatgacgag gcggacggtg tcgggcgtgt
     1921 tctgaccggc gcgtatgctg cggataaagg ggtcgtccgc cataactttt tgggaaagtc
     1981 cgtgcaatac ggtattgatg ttcgcgtttt gtatatcgac gaccagcctg cccggattgt
     2041 cgagcgtgaa gtgttggtat ttgagcgcgg cggtgctttc cagcgtcagg cgggtgtagg
     2101 tgtgcgacgg ccatatccgt acggcggtaa actgcggggc gcgtaccgtt ttggcaacgg
     2161 cggatgcgat ggggcttagg gcgaacagtg tgccggcggt gcggcggatg atttgtcttc
     2221 gtgtcagttt ggtcatagcg gcaggctttc gcgtcctcgt tcggtatggg cggtcagcag
     2281 gcattttctg ccgtcgccgt cgtgtgtcaa tgttgcggtg atgtcggcag gcggggtaaa
     2341 ttccccacct tgttgcggcc attcgatcag gcagacgctg tttgcggcaa acagttcgtc
     2401 caatccggca tcttcccatt cttcggggaa cgagaagcgg tagaggtcga aatggtgcaa
     2461 ggtgaagcgt tccagcagat aagattcgac gatggcgtaa gtcgggcttt tgactgcgcc
     2521 ctgatgaccc aatccgcgca ggatgccgcg tgtcagcgtg gttttgcccg cacccaaatc
     2581 cccctcgaga taaatgacca gcggcgcgtt taaacgggaa gaccacgccg cacccaaatc
     2641 gagtgtggcg gcttcgtcgg caaggaatcg ggagatagag ggtaaatcag acatggaaac
     2701 ggtttgttgt aaggtctagg gtattatggg cagttttgca ggttttgcaa actttgcacc
     2761 cgaggggcgg atgcttcttg tccgagcatt ataacagcca aatccgcgtt ctgctttcag
     2821 acggcaacgg ctgtcaagaa aaagcggcgc gtgtacaata cgcggattgt atgtttagga
     2881 cggattggaa aaagaatgga aaatatcggc aggcagcgac ccatcggcgt ttttgactcg
     2941 ggaatcggcg gtttgaccaa tgtgcgagcg ctgatggaac ggctaccgat ggagaacatc
     3001 atttatttcg gcgacacggc gcgcgtgcct tacgggacga aatctaaggc gaccatcgaa
     3061 aatttctcga tgcagattgt cgatttttta ttggaacacg atgtcaaggc gatggttatc
     3121 gcgtgcaata cgattgcggc ggtggcgggg cagaaaatcc gtcaaaaaac cggcaatatg
     3181 cccgttttgg acgtgatttc cgccggcgcg aaagccgcgc tggcaacgac gcgcaacaat
     3241 aaaatcggca ttatcgccac caatacgaca gtcaacagca acgcctatgc gcgcgccatc
     3301 cacaggaaca accccgatac gctcgtccgc acgcaggccg cgccgctgct cgtccctttg
     3361 gtggaagagg gctggttgga acacgaagtt acccgcctga ccgtatgcga atacctcaaa
     3421 ccattgcttg cagacggcat cgatacgctg gtgttgggct gcacgcactt tcccttgctc
     3481 aagcccttaa tcggcaggga ggcgggcaat gtcgcgctgg tcgattccgc gattacgact
     3541 gccgaagaaa ccgcacgtgt gctggcacag gaagggttgc tcaataccga caacaacaat
     3601 cccgactacc gtttttacgt cagcgatatt cctttgaaat tcagaaccat cggcgagcgt
     3661 tttctgggca ggacgatgga gcagattgaa atggtgtctt tgggttaaaa cgatgacgga
     3721 aagctgcccg agattacaga aacct
//
LOCUS       DAWFJR010000140         3541 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWFJR010000140 DAWFJR010000000
VERSION     DAWFJR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.140"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            206..670
                     /locus_tag="WGR04_001998"
     CDS             206..670
                     /locus_tag="WGR04_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0795478.1"
                     /translation="MNHGTWPKNNTSAGVASSATDIKGKYVQSVTVANGVITAQMASS
                     NVNNEIKSKKLSLWAKRQNGSVKWFCGQPVTRNDTATATDVAAANGKTDDKINTKHLP
                     STCRDDSSTGCIETPRADFKHFQKISRYRVLPESRQMAEKLRHSRKSGNLVR"
     gene            829..1341
                     /locus_tag="WGR04_001999"
     CDS             829..1341
                     /locus_tag="WGR04_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0795479.1"
                     /translation="MAEGQKSAVTEYYLNHGEWPGDNSSAGVASSATDIKGKYVQSVT
                     VANGVITAQMASSNVNNEIKGKKLSLWAKRQNGSVKWFCGQPVTRNKAATDVTADSGN
                     DKIDTKHLPSTCRDDSSVVCTKTPGFAFKYFQKISRYRVLPESRQMTKKLRHSRAGGN
                     LGLSAQKLIG"
     gene            1387..1743
                     /locus_tag="WGR04_002000"
     CDS             1387..1743
                     /locus_tag="WGR04_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0795480.1"
                     /translation="MTGFNDAAGVASSSDIKGIYVEKVEVAKGVITATMLSSNVNNEI
                     KGKKLSLWAKRQDGSVKWFCGQPVTRNKAATDANGVTADTNADKKIDTNHSHEPTSRH
                     SRAGGNPGLSVRKLIG"
     gene            1789..2157
                     /locus_tag="WGR04_002001"
     CDS             1789..2157
                     /locus_tag="WGR04_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0795481.1"
                     /translation="MTGFNDAAGVASSSDIKGIYVKSVEVKNGVITATMLSSGVNNEI
                     KGKKLSLWAKRQDGSVKWFCGQPVKRANADSATDVTADSGNDKIDTKLKKPRPLQNSF
                     PSRQPKPKHRFSAVFAPNTS"
     gene            complement(2221..3228)
                     /locus_tag="WGR04_002002"
                     /pseudo
     CDS             complement(2221..3228)
                     /locus_tag="WGR04_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            3211..>3541
                     /locus_tag="WGR04_002003"
     CDS             3211..>3541
                     /locus_tag="WGR04_002003"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795482.1"
                     /translation="MKEGAHGKSPKCLGGNLGDFGEFCKGLNLQIGFKTIRPNSSASK
                     NGMPIYSNVSRREIFDLYEQYSGQRPDFRNIPNKGQLSSTTITSGGGFAFEVQLSLTE
                     KGQYAVAY"
BASE COUNT          943 a          954 c          848 g          796 t
ORIGIN      
        1 tcggtaaaat ggttctgcgg acagccggtt gcgcgcgacg ccaccgccac cgacgccgac
       61 gtcaccgccg ccagcgacac caccgccaac atcgacacca agcacctgcc gtcaacctgc
      121 cgcgatgatt catctgccgt ttgcacaaaa acacctggtt tcgctttcaa atacttccaa
      181 aaactcagcc gtcaccgagt attacctgaa tcacggcaca tggccgaaaa acaacacttc
      241 tgccggcgtg gcatcctccg ccaccgacat caaaggcaaa tatgttcaaa gcgttacagt
      301 cgcaaacggc gtcattaccg cccaaatggc ttcaagcaac gtaaacaacg aaatcaaaag
      361 caaaaaactc tccctgtggg ccaagcgtca aaacggttcg gtaaaatggt tctgcggaca
      421 gccggttacg cgcaacgaca ccgccaccgc caccgacgtc gccgccgcca acggcaagac
      481 cgacgacaaa atcaacacca agcacctgcc gtcaacctgc cgcgatgatt catctaccgg
      541 ttgcatagaa acaccacgcg ccgatttcaa acactttcaa aaaatcagcc gttaccgagt
      601 attgcccgaa tcacggcaaa tggcagaaaa acttcgtcat tcccgcaaaa gcgggaatct
      661 agttcgttga gtttcagtta tttagaataa attttgaaac tttaatcccg tcattcccac
      721 gaaagtggga atccagaacg caaaatctaa agaaaccgtt ttacccgata agtttccgta
      781 ccgacagacc tggattcccg cctgcgcggg aatgacgaag ctatcctttt ggccgaaggt
      841 caaaaatcag ccgtcaccga gtattacctg aatcacggcg aatggcccgg cgacaacagt
      901 tctgccggcg tggcatcctc cgccaccgac atcaaaggca aatatgttca aagcgttaca
      961 gtcgcaaacg gcgtcattac cgcccaaatg gcttcaagca acgtaaacaa cgaaatcaaa
     1021 ggcaaaaaac tctccctgtg ggccaagcgt caaaacggtt cggtaaaatg gttctgcgga
     1081 cagccggtta cgcgcaacaa agccgccacc gacgtcaccg ccgacagcgg caacgacaaa
     1141 atcgacacca agcacctgcc gtcaacctgc cgcgatgatt catctgtcgt ttgcacaaaa
     1201 acacccggtt tcgctttcaa atacttccaa aaaatcagcc gttaccgagt attgcccgaa
     1261 tcacggcaaa tgaccaaaaa acttcgtcat tcccgcgcag gcgggaatct aggtttgtcg
     1321 gcgcagaaac ttatcgggta aaaaggtttc tccagtcctg aatcctagat tcccactttc
     1381 gtgggaatga cgggatttaa tgatgccgcc ggcgtggcat cctcctccga catcaaaggc
     1441 atatatgttg agaaagttga agtcgcaaaa ggcgtcatta ccgccacaat gctttcaagc
     1501 aacgtaaaca acgaaatcaa aggcaaaaaa ctctccctgt gggccaagcg tcaagacggt
     1561 tcggtaaaat ggttctgcgg acagccggtt acgcgcaaca aagccgccac cgacgccaac
     1621 ggcgttaccg ccgacaccaa tgccgacaag aagatcgaca ccaatcattc ccacgaacct
     1681 acatcccgtc attcccgcgc aggcgggaat ccaggtctgt cggtacggaa acttatcggg
     1741 taaaaaggtt tctccggtcc tgagttctag attcccactt tcgtgggaat gacgggattt
     1801 aatgatgccg ccggcgtggc atcctcctcc gacatcaaag gcatatatgt taaaagcgtt
     1861 gaagtcaaaa acggcgtcat taccgccaca atgctttcaa gcggcgtaaa caacgaaatc
     1921 aaaggcaaaa aactctccct gtgggccaag cgtcaagacg gttcggtaaa atggttctgc
     1981 ggacagccgg ttaagcgcgc caacgccgac agcgccaccg acgtcaccgc cgacagcggc
     2041 aacgacaaaa tcgacaccaa actaaagaaa ccgagacctt tgcaaaattc ctttccctcc
     2101 cgacagccga aacccaaaca caggttttcg gctgttttcg ccccaaatac ctcctaattt
     2161 tacccaaata cccccttaat cctccccgga tacccgataa tcaggcatcc gtgctgcctt
     2221 ttaggcggca gcgggcgcac ttagcctgtt ggcggctttc aacaggttca aacacatcgc
     2281 cttcagatgg ctttgcgcac tcactttaat cagtccgaaa taggttgacc gcgcatagcg
     2341 gaatttacgg tgcagcgtac cgaagctttg ttcgaccaca taacgggtct tcgataaata
     2401 ccggttgcgt ttggtttgcg tttccgacag cggacggttg cggcaggctt tgcgcataat
     2461 gccgtcctgc aactgatgtt cttccagatg ttgccggttt tccgcactgt catagccttt
     2521 gtcggcatag acggtcgtac ctttgggcag tccttccaac aacggcgaca ggtgtttgca
     2581 ctcatgggca ttggcggggg tgatgtgcag tttctcgata tagccttccc catcggtacg
     2641 ggtatgttgt ttgtaaccga gtctgtagag gccgtttttc tttgtccaac gggcatcgct
     2701 gtctttactc ggtgtggttt ggccgctgac ttgtcctttg ttatcgactt ctatagcctg
     2761 gtgctgtttg ctgccggcgg tctgaataat ggtggcgtca acgacggcgg cggatgcttt
     2821 ctctactttt aggccttttt cggtcagttg gcagttaatc agttccagca attcggacag
     2881 ggtgtcgtct tgcgccagcc aattgcggta acggcataaa gtactgtaat cggggatgct
     2941 cagttcgtca aagcggcaaa acaggttgaa gtcgatgcgg gtaatgaggc tgtgttcgag
     3001 ttcgggatcg gagagactgt gtcattgtcc gagcaggacg gctttgaaca tggacaacag
     3061 cgggtaggcg ggacggccgc ggtgatctct aaggtaacgg gttctttgac gattcaggta
     3121 ttgttcgatc ggctgccaat caatcacctg gtccaacttc aatagcggga aacggtcgat
     3181 gtgtttagca atcatggctt gggcggtttg ttgaaagaag gtgctcatgg aaaatctcct
     3241 aaatgtcttg gtgggaattt aggggatttt ggggaatttt gcaaaggtct caacttacaa
     3301 attggtttta aaacgatcag accaaattca tctgccagca aaaatggaat gccgatatat
     3361 tctaacgtaa gtcgaagaga aatatttgat ttatatgaac aatattcagg gcagaggcct
     3421 gattttagga atattccgaa taaagggcaa ttatcttcta cgactattac atcaggaggc
     3481 ggattcgcat ttgaagtgca actttcccta acagaaaaag gccagtatgc ggtagcatac
     3541 g
//
LOCUS       DAWFJR010000141         3482 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWFJR010000141 DAWFJR010000000
VERSION     DAWFJR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.141"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(436..1359)
                     /locus_tag="WGR04_002004"
     CDS             complement(436..1359)
                     /locus_tag="WGR04_002004"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ0795483.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRNCG"
     gene            complement(2212..2622)
                     /locus_tag="WGR04_002005"
     CDS             complement(2212..2622)
                     /locus_tag="WGR04_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0795484.1"
                     /translation="MDSRFCGNDESILLAEGQKSAVTEYYLNHGIWPGGNSSAGVATS
                     SEIKGKYVQSVTVAKGVITAEMKSDGVNNEIKGKKLSLWAKRQNGSVKWFCGQPVTRN
                     ANNADTVAAAAAANDKKIDTKHLPSTCRDDSSTG"
     gene            complement(3028..3420)
                     /locus_tag="WGR04_002006"
     CDS             complement(3028..3420)
                     /locus_tag="WGR04_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0795485.1"
                     /translation="MEEGAHGKSPKCHGGNLGDFGEFCKGLGKYVKEVTVANGVVTAT
                     MLSSGVNNEIKGKKLSLWAKRQDGSVKWFCGQPVTRTGTTTTNDEVTAASGTGTANID
                     TKHLPSTCRDAASAVCIETPPTAFYKNT"
BASE COUNT          850 a          808 c          867 g          957 t
ORIGIN      
        1 ttagaataac agcaatattc aaagattatc tgaaagtccg ggattctaga ttcccacttt
       61 cgtgggaatg acggcatcag tctgccgttt acagcacggt ttctttagat tttacgttct
      121 agattcccgc ctgcgcggga atgacgaatc catccatacg gaaacctgca ccacgtcatt
      181 cctacgaacc tacatcccgt cattcccaca aaaacagaaa accaaaaaca gaaaccgctc
      241 tgccgtcatt cccgcgcagg cgggaatcta gaccttagaa caacagcaat attcaaagat
      301 tatctgaaag tccgggattc tagattccca ctttcgtggg aatgacgaat tttaggtttc
      361 tgattttggt tttctgtttt tgtaggaatg atgaaaattt aagttttagg aatttaccgg
      421 aaaaaacaga aagcgttatc cacaatttct gatgttcagc tcgtgaaatg cgtcgggcaa
      481 atcatcgctg tcggcaaatt ccacccggtc gtaagccgtt tcgtctgcca aaaccgcgcg
      541 caagagtgcg ttgttgatgg cgtgtcccga tttgtagcct tcaaacgcgc cgacaatcgg
      601 atgtccgacg atatacaaat caccgatggc atcaaggatt ttgtggcgca caaactcatc
      661 gggatagcgc aagccttcag gattcaggac atccgtgtcg tcaatcacga tggcgttgtt
      721 caaattgccg cccaaaccca gattgtgggc gcgcatcatt tccacttcgt gcataaagcc
      781 gaaggtgcgc gcgcgcgcga tttcgtcgat gtaggatttg ccggcgaaat caatttcaaa
      841 agtgggcgag ctgcggttga aaaccggatg gtcgaattcg atggtcagcg ttaccttaaa
      901 gccgtcatac ggcgtaaagc gcacccattt gcccgcttct ttgatttcga caggcttgag
      961 gattttcaaa aaacgctttt gcgccttttg atcgaccacg cccgcatctt gcaaaaggta
     1021 aataaacggc aggctggagc cgtccataat cgggatttcg ggtgcgttca gctcaatcag
     1081 cgcgttgtcg ataccgtagg cggacagcgc ggacataatg tgttcgattg tgccgacgcg
     1141 cacgcctttg tcggtaacga tggtggagga aaggcgggta tcgttgatca aataaggggt
     1201 cagcttgatt tgttcgccca tctcgccgtc caaatcggta cggcggaagg aaatcccgct
     1261 gttttcaggc gcggggtgca gggtcagtgc gacccgttcg ccggaatgca ggccgactcc
     1321 ggtaacgctg atggatttcg ccaaagttct ttgcagcata aaccgctccc ttatcaatgg
     1381 ggtaagtttt ggaataatac gataaaaccg gaaaaacaag ctatgttttt ccatagtatt
     1441 tgccaatgta tccgttttca atacgtaagc cgcataaaaa tgtatagtgg attaacaaaa
     1501 atcaagacaa ggcgacgaag ccgcccctcc cgaaaaacgc aaaaaatgcc gtccgaaaac
     1561 ctttcggacg gcattttcgc gtgaaccgtc attcccacaa ggacaaaaaa ccaaaacaga
     1621 aaaccaaaaa cagaaaccta aaattcgtca ttcccacgaa agtgggaatc tagttcgttg
     1681 agtttcagtt atttagaata aattttgaaa cttaaatccc gtcattccca tgaacctaca
     1741 ttccgtcatt cccgcgcagg cgggaatcta gtctgttcgg tttcagttat ttccgataaa
     1801 tgcctgttgc ttttcatttc tagattccca ctttcgtggg aatgacggga ttttaggttt
     1861 ctgtttttgg ttttctgttt ttgagggaat gatgaaattt taagttttag gaatttatcg
     1921 ggagcaacag aaaccgctcc gccgtcattc ccgagtaggc gggaatccag tccgcttgtt
     1981 cggtaaatga gagggcggat tgcgcgcctg tcagataaac cccgtgttta aacgggcggc
     2041 aatgaggtac gcgcagagcc ttgaagcgca atcgatatat tattttcagc caaaacggac
     2101 gcccccgctt gccttgcaaa cccttaaaaa ggaagccacc cggattaatc cgagtggccg
     2161 tggaaaatca cttaccgctt gatttattta aaatttatgg tataatttgc cttatccggt
     2221 agatgaatca tcgcggcagg ttgacggcag gtgcttggtg tcgatcttct tgtcgttggc
     2281 ggcggcggcg gcggcgacgg tgtcggcgtt gttggcgttg cgcgtaaccg gctgtccgca
     2341 gaaccatttt accgaaccgt tttgacgctt ggcccacagg gagagttttt tgcctttgat
     2401 ttcgttgttt acgccgtctg atttcatttc ggcggtaatg acgccttttg cgactgtaac
     2461 gctttgaaca tatttgcctt tgatttcaga ggaggttgcc acgccggcag aactgttgcc
     2521 gccgggccat atgccgtgat tcaggtaata ctcggtgacg gctgattttt gaccttcggc
     2581 taaaaggatg gattcgtcat tcccgcaaaa gcgggaatcc agccttgtcg gtacggaaac
     2641 ttatcgggaa aacggtttct tgagattttg cgttctggat tcccactttc gtgggaatga
     2701 cgggattaaa gtttcaaaat ttattctaaa taactgaaac tcaacgaact agattcccgc
     2761 ttttgcggga atgacgaatt ttaggtttct gttttgggtt ttctgttttt gagggaatga
     2821 tgaaatttta ggtttctgtt tttggttttc tgtccttgtg ggaatgatga aattttaagt
     2881 tttaggaatt tatcgggagc aacagaaacc gctccgccgt cattcccgcg caggcgggaa
     2941 tccagcctcg tcggcacgga aacttatcgg gaaaacggtt tcttgagatt ttacgtccta
     3001 gattcccact ttcgtgggaa tgacggttta ggtattttta tagaaagccg taggtggtgt
     3061 ttctatgcaa acggcagatg ctgcgtcgcg gcaggttgac ggcaggtgct tggtgtcgat
     3121 gttggcggtg ccggtgccgc tggcggcggt gacttcgtcg ttggtggtgg tggtgccggt
     3181 gcgcgtaacc ggctgtccgc agaaccattt taccgaaccg tcttgacgct tggcccacag
     3241 ggagagtttt ttgcctttga tttcgttgtt tacgccgctt gaaagcattg tggcggtaac
     3301 gacgccgttt gcgactgtaa cttccttaac atattttccg agacctttgc aaaattcccc
     3361 aaaatcccct aaattcccac catgacattt aggggatttt ccatgagcac cttcttccag
     3421 caaaccgccc aagccatgat tgccaaacac atcgaccgtt tcccactatt gaagttggat
     3481 ca
//
LOCUS       DAWFJR010000142         3472 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWFJR010000142 DAWFJR010000000
VERSION     DAWFJR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.142"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            43..711
                     /locus_tag="WGR04_002007"
     CDS             43..711
                     /locus_tag="WGR04_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ0795486.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGTANNKCDSGKKEQGMLAFGDKNGNK
                     GYDNDTADVLLRSVVLNDDINDKRINYAFNHIAFGKTQPTADRVVWTFNQNGTFGYTN
                     DQHLTSQSSFSYSDGYIQIVLTDARAVSDADKKFRSAVVLINSSGRVEVCPRNDRRTV
                     CQYK"
     gene            741..1361
                     /gene="pilV"
                     /locus_tag="WGR04_002008"
     CDS             741..1361
                     /gene="pilV"
                     /locus_tag="WGR04_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ0795487.1"
                     /translation="MKNNDCLRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNHTLPHTLSAVDGEFA
                     IDAIKTKTQLAEAQLKRFSYELKNALPDAAAIHYAVCKDSSGNAPTLSGNAFSSNCDN
                     KANGDTLIKVLWVNDSAGDSDISRTNLEVSGDNIVYTYQARVGGRE"
     gene            1358..2347
                     /locus_tag="WGR04_002009"
     CDS             1358..2347
                     /locus_tag="WGR04_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ0795488.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLAIQQDLRNAATLIVRDARMAGSFGCFNMSEHIGSDVVSNVVQKNPL
                     FSLKKRNSTNKLIPITESLNIRYPGFTQRLNALIFEYGIDDVNASADTTVVSSCAKIA
                     KPGKKISTLQEAKSALQITNDDKQNGNITRQRHVVNAYAVGKIAGEEGLFRFQLNDDG
                     QWGNPQLLVKKINKMDIRYIYLLVENPGIPPTNSPRYIYGCPEDDDAGKEEKFKYTGT
                     FDSSTNAVTPAGVEVLLSSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2326..2919
                     /locus_tag="WGR04_002010"
     CDS             2326..2919
                     /locus_tag="WGR04_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ0795489.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCTAVNVRT
                     NNDNEEAFDNIVVQGKPTVEAVKRSCTAKSTGLCIDNKGMEYKKGKGSVSKPPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2924..3397
                     /locus_tag="WGR04_002011"
     CDS             2924..3397
                     /locus_tag="WGR04_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ0795490.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQTIKNKLERFVSGYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASAQAHLETLSSDVGCEAFSNRKK"
BASE COUNT         1014 a          690 c          858 g          910 t
ORIGIN      
        1 aatttaatcc acgatacatt accagttaac gttctattgc ttatgtgtac acgaaaacaa
       61 caaggtttca cgctaacaga gctgctcatc gtgatggtca ttgcagccat tatggcgatg
      121 atagccctcc ccaatatgag ccaatggatt gcatcccgcc gcattgccag tcacgcggag
      181 cggattgcca accttttgcg tttctccagg ggcgaagccg tccggctcaa tctccctgtc
      241 tatatctgtc ctgttcaagt taaaaaagac ggtacggcca acaataaatg tgactccggc
      301 aagaaggagc agggaatgtt ggctttcggc gacaaaaacg gcaataaggg atatgacaat
      361 gatacggcgg atgttcttct ccgcagtgtg gtattgaatg atgatatcaa tgataagcgg
      421 attaattatg ccttcaacca tatcgctttc ggtaaaacgc agccgaccgc tgaccgtgta
      481 gtttggacat tcaatcaaaa cgggacgttc ggttatacga acgaccagca tcttacaagc
      541 caatccagct tttcttattc cgacggttat atccaaatcg tgctgacaga tgcgagagca
      601 gtttcagatg ccgataagaa attccgttcg gcggtggttt tgattaacag cagcggcagg
      661 gttgaggttt gtcctagaaa cgataggcgt accgtatgcc aatataaatg attgttttag
      721 tcggtttggg aaataaaaaa atgaagaata atgattgctt gcgcctgaaa aatccccagt
      781 ccggtatggc gctgatagaa gtcttggtcg ctatgctcgt tctgaccatc ggtattttgg
      841 cactattgtc tgttcagttg cggacagtcg cttccgtcag ggaggcagag acgcaaacca
      901 tcgtcagtca aatcacgcaa aacctgatgg aaggaatgtt gatgaatccg accattgatt
      961 cggacagcaa caagaaaaac tataatcttt acatgggaaa ccatacacta ccgcatacac
     1021 tatcagctgt ggatggtgag tttgcgattg atgccataaa aactaagacg cagttggcag
     1081 aggcacaatt gaagagattt agttatgagc tgaaaaatgc cttgccggat gcggcagcca
     1141 tccattacgc cgtctgcaag gattcgtcgg gtaacgcgcc gacattgtcc ggcaatgctt
     1201 tttcttcaaa ttgcgacaat aaggcaaacg gggatacttt aattaaagta ttgtgggtaa
     1261 atgattcggc aggggattcg gatatttccc gtacgaatct tgaggtgagc ggcgacaata
     1321 tcgtatatac ttatcaggca agggtcggag gtcgggaatg agacgtaaaa tgctaaacgt
     1381 accaaaaggc agttatgatg gtatgaaggg ttttaccatt attgaatttt tggttgcggg
     1441 catgctcagt atgattgtcc tgatggcggt cggatcgagt tacttcacgt cccggaaatt
     1501 aaatgatgcg gcaaacgagc gtcttgctat tcaacaggat ttgcggaatg cggcaacatt
     1561 aattgtccgc gatgcaagaa tggcggggag cttcggttgt ttcaatatgt ctgagcatat
     1621 cggaagtgat gttgtttcca atgtggtgca aaaaaaccct cttttttcct taaaaaaaag
     1681 gaacagtaca aataaactta tccccataac ggaatcttta aatatcagat atccgggttt
     1741 tacccagcgc cttaacgcat tgattttcga atacggaatc gatgatgtta atgcaagcgc
     1801 cgatactacc gtcgtcagca gctgtgccaa aatagcaaaa ccgggtaaga aaatatctac
     1861 cttgcaagaa gcaaagagtg cattacagat tactaatgat gataaacaaa atggaaatat
     1921 cacccgtcaa aggcatgtgg tcaatgccta tgcggtcggc aagattgccg gtgaggaagg
     1981 tttgttccgc ttccaattga atgatgatgg gcagtggggc aatcctcagt tgttggttaa
     2041 aaagattaat aaaatggata tacggtatat ctatcttctc gtggaaaatc ctggtatacc
     2101 gcctaccaat tctccccggt atatttacgg ttgccctgaa gatgacgatg ccggcaaaga
     2161 ggaaaaattc aaatatacgg gtacattcga cagctccaca aatgctgtta cgcccgccgg
     2221 ggtggaggtt ttattgagta gcggtactga taccaagatt gccgcttctt cagacaatca
     2281 tatttatgct taccgtatcg atgcgacaat acgcggggga aatgtatgcg caaacagaac
     2341 actttgacgg gaatcccgac ttctgacgga cagagggggt ttgcactgtt tatcgtgctg
     2401 atggtgatga tcgtcgtggc ttttttggtt gtaactgccg cgcagtctta caataccgag
     2461 cagcggatca gtgccaacga atcagacagg aaattggctt tgtctttggc cgaggcggct
     2521 ttgcgggaag gcgaacttca ggttttggat ttggaatatg atacggacag taaggttaca
     2581 tttagcgaaa actgtggaaa aggtctgtgt accgcagtga atgtgcggac aaataatgat
     2641 aatgaagagg cttttgacaa tatcgtggtg caaggcaagc ccaccgttga ggcggtgaag
     2701 cgttcttgca ctgcaaaatc tacaggcctg tgcattgaca ataaagggat ggaatataag
     2761 aaaggcaagg gaagcgtcag caaaccacca cgctatatta tcgaatattt gggcgtgaag
     2821 aacggagaaa atgtttatcg ggttactgcc aaggcttggg gtaagaatgc caataccgtg
     2881 gtcgtccttc aatcttatgt aagcaataat gatgagtaat aaaatggaac aaaaagggtt
     2941 tacattgatt gagatgatga tagtcgtcgc gatactcggc attatcagcg tcattgccat
     3001 accttcttat caaagttata ttgaaaaagg ctatcagtcc cagctttata cggagatggt
     3061 cggtatcaac aatatttcca aacagtttat tttgaaaaat cccctggacg ataatcagac
     3121 catcaagaac aaactggaaa gatttgtctc aggctataag atgaatccga aaattgccga
     3181 aaaatataat gtttcggtgc attttgtcaa taaggaaaaa ccaagggcat acagcttggt
     3241 cggcgttcca aagacgggga cgggttatac tttgtcggta tggatgaaca gcgtgggcga
     3301 cggatacaaa tgccgtgatg ccgcttctgc ccaagcccat ttggagacct tgtcctcaga
     3361 tgtcggctgt gaagccttct ctaatcgtaa aaaataaggt tgttttgcca ataccgtctg
     3421 aaaatcaatg ttcagacggt atttttatgg gtatagtgga ttaacaaaaa tc
//
LOCUS       DAWFJR010000143         3465 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWFJR010000143 DAWFJR010000000
VERSION     DAWFJR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.143"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            207..1679
                     /gene="pyk"
                     /locus_tag="WGR04_002012"
     CDS             207..1679
                     /gene="pyk"
                     /locus_tag="WGR04_002012"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ0795491.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRTGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQVEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            1989..3383
                     /locus_tag="WGR04_002013"
     CDS             1989..3383
                     /locus_tag="WGR04_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ0795492.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNERHSFGAGIIAQHTSAELRKYADWGIMEKAKALKETPPNPTKAAQIKADGHA
                     DVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADDAMAKQLWDANK
                     LALLGYTPSEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFEKE
                     KTIVNGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMNSL
                     PDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARFKN
                     HADIIGLQYTYKFK"
BASE COUNT          899 a         1058 c          868 g          640 t
ORIGIN      
        1 aataaattta gcctaagtca taaaaccgtg taattttgcg ccaaaaaagg cggtttgcag
       61 gtaaaatatc aaactattaa tttaaatcat atttttacag aatattccgc cgcgttcatc
      121 aaatggacat caaaccggtt ccaaatttct gtaattttgt aacaaaatac cgcaaaacac
      181 ccgattgaga ccaaaaggac tttcatatga accagacaag ccgcgatctg acccgcatca
      241 gccacaacac taaaatcgtc gccacccttg ggccgggcag caacaacgtc gaactgttgg
      301 aagacatgat ccgcgtcggc ggtctgaacg tcgtccgctt caacttcagc cacggcacgc
      361 ccgaattcca tcaggaaaac gcccgcatcg tgcgcgaggc ggcaaaacgc accggacagg
      421 aaatcgccat cattgccgac ctgcagggcc cgaaaatccg cgtgggcaaa atcgccggcg
      481 gcggcatcga attgaacaaa ggcgaaacgc tggtactgga tgccgcgctc gaaggcgaag
      541 gcacgcgcga ggcggtcggt ttggactacc gcgacctgcc cgacgacgtt gccgcaggcg
      601 atgtcttgtg gctggacgac ggcctgctga ccctgaccgt ggaatccgtc gaaggcagca
      661 ggattatcac aagggtggaa aacagccacg tcctgaaaag caacaagggc atcaacaaac
      721 gcggcggcgg tctgtccgca ggcgcgttga ccgaaaaaga cttccgcgac ctgaaaaccg
      781 cgattgccat cggttgcgac tatctcgcca tcagctttgt gaaatccgcc gaagatttgc
      841 acatcgcgcg cgccaaagtc gaagaagaaa tgaaaggcag tacggccgtg cgccccggtt
      901 tggtttccaa aatcgaacgc gtagaagcga ttgaaaacct tgatgaaatc atcctcgccg
      961 gcgacggcat tatggttgca cgcggcgact tggcggtcga agtcggacac gccgccgtcc
     1021 ccgccctgca aaaacggatg atccgccgcg cccgcgagtt gcgccgcttc agcattacgg
     1081 cgacgcaaat gatggaatcg atgattacca accccgtgcc gacccgcgcg gaagtcagcg
     1141 atgtggcaaa cgcggtattg gacggcaccg atgcggtgat gtgttccgcc gaaaccgccg
     1201 tcggcgcgta tccgtttgaa accgtcagcc aaatggcgat tatctgcgcg gctgcggaaa
     1261 aagagcagga ttcgctcaac ggcgttgccg aacaggtcga gtatcccgaa gcggtcagca
     1321 ccaacctggc ggttgccggc ggtgcggtca gcgtggcgcg cgcggttcac gccaaagcca
     1381 tcgtcgccct gaccgaaagc ggttcgaccg cctttgaaat cagccgccac aacatcaccc
     1441 tgccgatttt cgcgctgacc ccgagcgttt ccgcccaacg ccgtatggcg atgtaccggg
     1501 gcgtgcgccc gctgattttg gcaaccagca ccgaccacga cacggcgtta aacgaagtcg
     1561 aaaccatgct ggtggaacac aacatcctgc attccggcga ccaatacatc attaccagcg
     1621 gctcgcaaat gcgcgaatcc ggttcgacca atacgctgga agtgctgcgc gtcaaataat
     1681 ccgccctgag tggaaaatgc cgtctgaagt cgatgcccga ggcttcagac ggcatttttt
     1741 gcggcggcgc ggcggttcgg gcaaactctg aaaaaattgt gcaaatccgg caacatcgga
     1801 taaaatcgag tacctatact aaagcgaaac aaggcatttc cgactgcctt ttttatagtg
     1861 gattaaattt aaaccagtac ggcgttgcct tgccgtacta tctgtactgt ctgcggcttc
     1921 gtcgccttgt cctgattttt gttaatccac tatatttatc caccgtccgc ctttttacgg
     1981 aaaccgaaat gaccccttcc gcactgaaaa aaaccgtcct actgctcggc actgcctttg
     2041 ccgccgcatc cgcacaagcc tccggctacc acttcggcac acagtcggtc aacgcgcaaa
     2101 gcacggcaaa tgccgccgcc gcagaagccg ccgacgcatc gaccatcttc tacaaccctg
     2161 ccggcctgac caaactcgac agcagccaga tttccgtcaa cgccaacatc gtgctgccca
     2221 gcattcatta tgaggcggat tccgccaccg actttaccgg gcttcccgtc caaggttcga
     2281 aaagcggcaa aatcaccaaa accacggtcg cgccccacat ctacggcgca tacaaagtca
     2341 acgacaatct gaccgtaggc ttgggcgtgt acgtcccctt cggttctgcc accgaatacg
     2401 aaaaagattc cgtgttgcgc cacaacatca acaaactcgg tctgaccagc atcgccgtcg
     2461 aacctgtcgc cgcgtggaaa ctcaacgaac gccattcctt cggcgcaggc atcatcgccc
     2521 aacatacttc cgccgagctg cgcaaatatg ccgactgggg gattatggaa aaagcgaaag
     2581 cactaaaaga aacacccccc aatccaacta aagccgccca aatcaaagcc gacggacacg
     2641 ccgatgtcaa aggcagcgat tggggcttcg gctaccaact ggcgtggatg tgggacatca
     2701 acgaccgtgc gcgcgtgggc gtgaactacc gttccaaagt ctcacacacg ctcaaaggcg
     2761 atgccgaatg ggcggcagac gacgcaatgg cgaaacagtt atgggatgca aacaaactcg
     2821 cactgctcgg ctacacgcca agcgaaaaag cccgcgttaa aatcgttacg cccgagtctt
     2881 tgtccgtaca cggtatgtac aaagtgtccg acaaagccga cctgttcggc gacgtaactt
     2941 ggacgcgcca cagccgcttc gataaggcgg aactggtttt tgaaaaagaa aaaaccatcg
     3001 tcaacggcaa atccgaccgc accaccatca cccccaactg gcgcaacacc tacaaagtcg
     3061 gcttcggcgg ttcttatcaa atcagcgaac cgctgcaact gcgcgccggc atcgcttttg
     3121 acaaatcgcc cgtccgcaac gccgactacc gcatgaacag cctgcccgac ggcaaccgca
     3181 tctggttctc cgccggcatg aaataccata tcggcaaaaa ccacgtcgtc gatgccgcct
     3241 acacccacat ccacatcaac gacaccagct accgcacggc gaaggcaagc ggcaacgatg
     3301 tggacagcaa aggcgcgtct tccgcacgtt tcaaaaacca cgccgacatc atcggcctgc
     3361 aatacaccta caaattcaaa taaacggccc gccgtttgat tgtagaaatg ccgtctgaaa
     3421 cagatttccg tttcagacgg cattgtttta ggcgcgattt atagt
//
LOCUS       DAWFJR010000144         3426 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWFJR010000144 DAWFJR010000000
VERSION     DAWFJR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.144"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            586..1209
                     /locus_tag="WGR04_002014"
     CDS             586..1209
                     /locus_tag="WGR04_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0795493.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGTLLLSPLAQAGAQREETLADDV
                     ASVMRSSVGSINPPRLVFDNPKEGERWLSDMSARLARFVPDEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1320..2702
                     /locus_tag="WGR04_002015"
     CDS             1320..2702
                     /locus_tag="WGR04_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0795494.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFILGIACIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQVNQEGLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2706..3230
                     /locus_tag="WGR04_002016"
     CDS             2706..3230
                     /locus_tag="WGR04_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ0795495.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPAPDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          719 a          901 c         1029 g          777 t
ORIGIN      
        1 gtgagtgcgc aaagccatct gaaggcgatg tgtttgaacc tgttgaaagc cgccaacagg
       61 ctaagtgcgc ccgctgccgc ctaaaaggcg gcccggatgc ctgattatcg ggtatccggg
      121 gaggattaag ggggtatttg ggtagaatta ggcggtattt ggggcgaaaa cagccgaaaa
      181 cctgtgtttg ggtttcggct gtcgggaggg aaaggaattt tgcaaaggtc tcaagatata
      241 gtggattaaa tttaaatcag tacggcaagg cgaggcaacg ctgtactggt ttaaatttaa
      301 tctactatat ttccgcctgc tgccgcccga aaagcgtgat gcgctgatgg tgctgctgca
      361 taatccggac gacggataac cggtgaaaat gggaaatgcc cgggcggtgt tgcctgtgcc
      421 tgtttggaca agcgttcttg agggcggtaa aatcaaggtt tgcccgaaaa gggcagggcg
      481 atatgccgcg cttggatttt gtgcgcggtt tttcgcgttg gaagcaatca tgtctgtttg
      541 aatatttaat gccgtctgaa acgtccggac gcgcccccgt tccctatgaa aaaaccgacc
      601 gataccctac ccgttaatct gcaacgccgc cgcctgttgt gtgctgccgg cacgctgttg
      661 ctcagcccgc tggcacaagc cggcgcgcaa cgtgaagaaa cgcttgccga cgatgtggct
      721 tccgtgatga ggagttccgt cggcagcata aatccgccga ggctggtgtt tgacaatccg
      781 aaagagggcg aacgttggtt gtccgacatg tcggcacgtt tggcaaggtt tgtccccgat
      841 gaggaggagc ggcgcaggct gctggtcaat atccagtatg aaagcagccg ggccggtttg
      901 gatacgcaga ttgtgttggg actgattgag gtggaaagcg cgttccgcca gtatgcaatc
      961 agcggcgtcg gtgcgcgcgg tctgatgcag gttatgccgt tttggaaaaa ctacatcggc
     1021 aaaccggcgc acaacctgtt cgacatccgc accaacctgc gttacggctg taccatcctg
     1081 cgccattacc ggaatcttga aaaaggcaac atcgtccgcg cgcttgcccg ctttaacggc
     1141 agcttgggca gcaataaata tccgaacgcc gttttgggcg cgtggcgcaa ccgctggcag
     1201 tggcgttgat tttgaacccg cgccgcaacc gaaatacggc gaatcctgta taatccgaaa
     1261 atctgttcac tggaagttca gacggcattg caactgttga tgccgtctga aaaaatatga
     1321 tggcaagaga caaccgcatc caaatgtttc cgcacgaatg gcgcgccagt acgacgcttt
     1381 ccggcgtgta cgcgctgcgt atgctgggta tgttcctcgt gctgcccgta ttggcggtgt
     1441 atgccgcctc gctgcccggc gcggaaggca acaaaacgct ggtcgggctg gcaatgggca
     1501 tttacgggct gacacaggct ctgctgcaac tgcctttggg catcgcttcc gacaagttcg
     1561 ggcgcaagaa aaccatttat gtcgggctga ttgtgtttgc ggcgggcagc tttcttgccg
     1621 ccgccgccga tacgctgccc atgctggtcg ccgcacgcgc catacagggt gcaggggcgg
     1681 tcagtgcggc ggttaccgcg ctgctggcgg atttgacgcg cgacggcgta cgtacccgcg
     1741 caatggcgat gatcggtttg agtatcggtt tgacgttttc ggtcagcctc gtcgttgccc
     1801 ccgtgattgc cgacgcggtc ggcgttcgcg gactgtttat gctgaccggc attctgaccg
     1861 tcatcagcat cggcgtggtg gcgtggatga ctcccgatcc cgaagtttcc aagctgcacg
     1921 aagatacgca ggcgcagcct tcgcgcatag gcgaagtttt gaaaaaccgt aggctgctga
     1981 cgctcgattt cggcattttc gccctgcacg ccgcgcaaat ggcattgttt accgcgctgc
     2041 ctttcgcgat gacccagctc ggtttggaaa aaatccagca ctggaaagtc tatctgccct
     2101 cgaccattac gggattggtg gtaatggttc cgctgattat cgtcggtgag acgcgcaaca
     2161 agctcaaaca ggtttttatc ttgggtatcg cctgtattgc ggcggcgcag ctcggtttgc
     2221 tgtccggtat gcgctcggta ggcttgatta ccgcttattt ggttgtttac tttatcggtt
     2281 ttaatgtgtt ggaagcgagc ctgccgtcta tggtttccaa aatcgcgccg tccgacctga
     2341 agggtacggc gatgggcgtg tacaacacga tgcagtcgct cggactgttt gccggcggcg
     2401 cggcaggcgg tttgctgttt caaaaatacg gcttttccgg cgtgtttgcc ttttgcagca
     2461 tattgatgct gctgtggctg gtgattgccg ttttatcgcc tgcgcccaag cccgtcaaaa
     2521 acctcagtta ccctgtcggc ggcgtgtggc aggtcaatca ggaagggtta taccgcgcct
     2581 tgtcggagct tgagggcgtg gaagacatcg gtttcagttt cgacgggcag accgtctatc
     2641 tcaaagtgtt gcagaagggt ttcgatcagg ctgccgctga aaaaatcatc acaggagttt
     2701 aaaaaatgtc attgaacaaa gtcatcctta tcggccgcct cggacgcgat cccgaagtgc
     2761 gctatatgcc caacggcgag gcggtgtgta atttcagcgt cgccaccagc gaaacatgga
     2821 acgaccgcaa cggccaacgt gtagagcgca ccgagtggca caacatcacg atgtaccgca
     2881 aactggcgga aattgccggg caatacctca aaaaaggcgg gctggtttat ttggaaggca
     2941 gaatccaaag ccgcaaatac caaggtaaag acggcatcga gcgcaccgct tacgatattg
     3001 tcgccaacga aatgaaaatg ttgggcgggc gcaatgaaaa cagcggcggc gcaccttacg
     3061 aggaaggtta cggtcagagt caggaggctt accaacgccc cgcgcagcaa agccggcagc
     3121 ctgcccccga cgcgccgtcc catcctcaag aagcaccagc cgcgccgcgc cgccaacccg
     3181 tgccggccgc cgccccggtc gaggatattg acgacgacat cccgttctga attttacggc
     3241 agcacatcct cgcggaggga aatcataaag gacggagaaa ccttaaacct tacggggcag
     3301 ggtttctctt ttttgcattt gtgccggctt gttgctgccc cctgcgttgg gcggaacgtt
     3361 gcgattgcgc cgttctggcg ttttgaggca gtgatgcctg ccgcacgtcc ccgctgtttt
     3421 caggcg
//
LOCUS       DAWFJR010000145         3277 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWFJR010000145 DAWFJR010000000
VERSION     DAWFJR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.145"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(439..603)
                     /locus_tag="WGR04_002017"
     CDS             complement(439..603)
                     /locus_tag="WGR04_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795496.1"
                     /translation="MRFVVLASLQAAFSGRSCNESVIGFDVSLRLTALSLTLSHRERG
                     RVAVASLFAA"
     gene            634..957
                     /locus_tag="WGR04_002018"
     CDS             634..957
                     /locus_tag="WGR04_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0795497.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSVVLIELSD
                     PSEAV"
     gene            1071..3248
                     /locus_tag="WGR04_002019"
     CDS             1071..3248
                     /locus_tag="WGR04_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0795498.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKALDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTATTDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDLSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQGETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          803 a         1123 c          833 g          518 t
ORIGIN      
        1 tagggagagg gcagtaagcc gtaggcttac atcaaagccg ataacgcttt cacaaagcct
       61 gtgtgcactg aaaactgaaa ggtcgtctga aaacggagct tctgaaggaa gcagagtttc
      121 tgcgaagcta aaaccgaagc tgcaaaaaat cgaaaagcag cctgcacgtt gaaaatgccg
      181 cccaagcaaa cctgcggttt gccgccctct ccctagccct ctcccacagg gagaggggat
      241 tggggtgcag gctgtcttta aggttcaggc gatttttaac tcttgttgaa gctgcgattt
      301 cagaagctcc gttttagctt cgcagaaact ccgcttcctt cgaaagctcc gttttcagac
      361 gaccttttgg aacaccacag gcacacgcat cgaacggctg aatcaaagat tcagaccgat
      421 ggcagtccgc acccgagttt atgcggcaaa cagcgaggct acggcaaccc gccccctctc
      481 cctgtgggag agggttaggg agagggcggt aagccgcagg cttacatcaa agccgataac
      541 gctttcgtta caactccgcc cactgaaagc agcctgcaac gaagccaaaa cgacaaaccg
      601 catcgtaaac cacccaaccc ataggagaac cccatgcaaa acgaaaccat caacctgaaa
      661 cagcaccttg ccgccatcaa agaatactgg cagcccgaaa tcatcaaccg ccacgggttc
      721 caattccgtt tggtcaaact tttgggcgat tacggctggc atacgcacgg atacagcgac
      781 aaagtgctgt ttgccgtgga gggcgacatg gcggtggact tcgccgacgg cggcagcatg
      841 acgatacgcg agggcgagat ggcggtcgtg ccgaagtcgg tgtcgcaccg cccgcgttcg
      901 gaaaacggct gctcggtggt gctgattgag ttgtccgacc cgtccgaggc cgtctgaaaa
      961 cgaagtttcc gaaggaagct gagtttctgc gaagctaaaa gcagcctgca ccttcaatca
     1021 atatgccgaa aatacaaccc caccgcacac caacacacaa aggaaatccc atgacacgct
     1081 tcaaatattc cctgctgttt gccgccctgt tgcccgtgta cgcgcaggcc gatgtttctg
     1141 tttcagacga ccccaaaccg caggaaagca ctgaattgcc gaccatcacc gttaccgccg
     1201 accgcaccgc gagttccaac gacggctaca ctgtttccgg cacgcacacc ccgctcgggc
     1261 tgcccatgac cctgcgcgaa atcccgcaga gcgtcagcgt catcacatcg caacaaatgc
     1321 gcgaccaaaa catcaaagcg ctcgaccgcg ccctgttgca ggcgaccggc accagccgcc
     1381 agatttacgg ctccgaccgc gcgggctaca actacctgtt cgcgcgcggc agccgcatcg
     1441 ccaactacca aatcaacggc atccccgttg ccgacgcgct ggccgatacg ggcaatgcca
     1501 acaccgccgc ctatgagcgc gtagaagtcg tgcgcggcgt ggcggggctg ctggacggca
     1561 cgggcgagcc ttccgccacc gtcaatctgg tgcgcaaacg cccgacccgc aagccattgt
     1621 ttgaagtccg cgccgaagcg ggcaaccgca aacatttcgg gctgggcgcg gacgtatcgg
     1681 gcagcctgaa caccgaaggc acgctgcgcg gccgcctggt ttccaccttc ggacgcggcg
     1741 actcgtggcg gcagcgcgaa cgcagccgcg atgccgaact ctacggcatt ttggaatacg
     1801 acatcgcacc gcaaacccgc gtccacgcag gcatggacta ccagcaggcg aaagaaaccg
     1861 ccgacgcgcc gctcagctac gccgtgtacg acagccaagg ttatgccacc gccttcggcc
     1921 cgaaagacaa ccccgccaca aattgggcga acagccgcca ccgtgcgctc aacctgttcg
     1981 ccggcatcga acaccgcttc aaccaagact ggaaactcaa agccgaatac gactacaccc
     2041 gcagccgctt ccgccagccc tacggcgtag caggcgtgct ttccatcgac cacaacaccg
     2101 ccaccaccga cctgattccc ggttattggc acgccgaccc gcgcacccac agcgccagcg
     2161 tgtcattgat cggcaaatac cgcctgttcg gccgcgaaca cgatttaatc gcgggtatca
     2221 acggttacaa atacgccagc aacaaatacg gcgaacgcag catcatcccc aacgccattc
     2281 ccaacgccta cgaattttcc cgcacgggtg cctacccgca gcctgcatcg tttgcccaaa
     2341 ccatcccgca atacggcacc aggcggcaaa tcggcggcta tctcgccacc cgtttccgcg
     2401 ccgccgacaa cctttcgctg atactcggcg gcagatacag ccgttaccgc accggcagct
     2461 acgacagccg cacacaaggc atgacctatg tgtccgccaa ccgtttcacc ccctacacag
     2521 gcatcgtgtt cgacctgacc ggcaacctgt ctctttacgg ctcgtacagc agcctgttcg
     2581 tcccgcaatc gcaaaaagac gaacacggca gctacctgaa acccgtaacc ggcaacaatc
     2641 tggaagccgg catcaaaggc gaatggcttg aaggccgtct gaacgcatcc gccgccgtgt
     2701 accgcgcccg taaaaacaac ctcgccaccg cagcaggacg cgacctgagc ggcaacacct
     2761 actaccgcgc cgccaaccaa gccaaaaccc acggctggga aatcgaagtc ggcggccgca
     2821 tcacgcccga atggcagata caggcaggtt acagccaaag caaaacccgc gaccaagacg
     2881 gcagccgcct gaaccccgac agcgtacccg aacgcagctt caaactcttc actgcctacc
     2941 actttgcccc cgaagccccc agcggctgga ccatcggcgc aggcgtgcgc tggcagggcg
     3001 aaacccacac cgaccctgcc acgctccgca tccccaaccc cgccgccaaa gcccgcgccg
     3061 ccgacaacag ccgccaaaaa gcctacgccg tcgccgacat catggcgcgt taccgcttca
     3121 atccgcgcgc cgaactgtcg ctgaacgtgg acaatctgtt caacaaacac taccgcaccc
     3181 agcccgaccg ccacagctac ggcgcactgc ggacagtgaa cgcggcgttt acctatcggt
     3241 ttaaataaga tcgtctgaaa acggagtttc tgcgaag
//
LOCUS       DAWFJR010000146         3140 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWFJR010000146 DAWFJR010000000
VERSION     DAWFJR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.146"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(190..2322)
                     /locus_tag="WGR04_002020"
     CDS             complement(190..2322)
                     /locus_tag="WGR04_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ0795499.1"
                     /translation="MSIVSAPLPALSALIILAHYHGIAANPADIQHEFCTSAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPIKRLAMATLPALVWCDDGNHFILAKTDGEGEHAQFLIQ
                     DLVTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
BASE COUNT          911 a          854 c          698 g          677 t
ORIGIN      
        1 gggattttgg ggaattttgc aaaggtctct agatgagtga aaaagaagtg caggctgcct
       61 aaaaagacag aaaaagtctt tcaggcagcc tgcactttgg tttcatttca gtcagtaaac
      121 ccagtaaacg acggtctgaa aacgcagaac gttacgaaaa aagcagccta cacgcccatc
      181 ccccgccttc tacccgttct gtaaatcata cagatagcgg taatatccgt tcggcttcgc
      241 cagcaattcc tgctgtgttc ccgcttccac aatcctgcct ttatccatgg caatgatccg
      301 gtgtgccgtt ttaacggtgg acagacggtg ggcgataatc agcaccgtcc ggttggcgca
      361 aatggcctgc atgttctgca taatcgctcg ttcactttca taatccagcg cgctggtggc
      421 ttcgtcaaaa atcagaatgc gcggattggt gattaacgcg cgggcaatcg caatacgctg
      481 ccgctgtccg cccgacaagc cggccccttg ttcgcccacc acggtgccgt agccttccgg
      541 cagctccata ataaactcgt gtgcgcccgc cagtttggct gcttcgataa tgcgttccag
      601 cggcataccc gtatccgtca gcgcgatatt gtcgcgtatg ctgcggttga gcagcacatt
      661 ctcctgcaag accacgccga cctgccgccg cagccaggca ggagcggcca aagccaaatc
      721 gttgccgtcc accaacaccc gtccctgctc cggtacatac agacgctgca ccaatttggt
      781 gagtgtggat ttgcccgacc ccgaacgtcc cacaatcccc agcacttccc ccgcccgaat
      841 ccgcaggttc aaatcctgca aaatcagcct gccgtccgcc ttatagcgga aatcgacatg
      901 ttcgaacgta atctcccccc ggatatcggg caaagccaaa tgcgaagacg cattctcggt
      961 cggcgcattc agaatatccc ccaaacgcgc caccgaaatc cccacctgct ggaaatcctg
     1021 ccacaactgc gccaaacgga taacaggcgc cgccacctgt cccgagagca tattaaacgc
     1081 aatcagctgc cccaccgtca gcttgccctc aattaccagc cgtgcgccaa tccacaacgt
     1141 cgccaccgtc accagcttct gaatcagctg caccccctgc tggccgacca ccgccaactt
     1201 cgttacccga aatcccgaag ccacataagc cgccaactga ttgtcccaac gctgcgtcat
     1261 ctgcggctcc accgccatcg cctttaccgt acccaccgca gtgatgcttt ctactaaaaa
     1321 cgactggttg tctgcattgc gcgcgaactt atcgttcaga cgcgtccgca gtatcggact
     1381 gataaatgcc gaccaaaacg cataggcagg caacgaagcc aataccaccc aagtcagagt
     1441 ggagctgtaa taccacatca ccgccagaaa gataaacgaa aacgccaaat ccaacaccga
     1501 agtcagcgcc tgaccggtca agaaattgcg aatctgctcc aattcccgca cccgagccac
     1561 cgtatcaccc actcgtctgt gctcgaaata ggataaaggc agggaaagca gatgccggaa
     1621 caaacgcgcg cccaattcca catcaatacg tgaagtcgta tgtgcaaaca gatacgtccg
     1681 caaaccgccc aacacaatct caaacagcga caccaccaac aaagccaccg acaccacatc
     1741 caaagtagag aatccccgat gtaccagcac cttgtccatc accacttgga aaaacagagg
     1801 cgtaatcagc gcaaacagct gcaacaccac cgacaccacc aatacttcaa aaaacaaccg
     1861 gcggtatttg attaccgccg gaataaacca ggtaaagtca aactttgcca aactgcccaa
     1921 taccgaagcg cgggaagcaa ccaatatcag tttgcccgaa tatctgttag aaaattcggc
     1981 aaaagacaat accgcagact tattcgtaac caaatcctgt atcaaaaatt gggcatgctc
     2041 accctcaccg tctgttttgg ccaaaataaa atggttgccg tcatcacacc ataccaatgc
     2101 gggtaaagtc gccatagcca aacgtttaat aggctggcgg actacctttg ccttcaatcc
     2161 caaagatttg gcggccaaaa gccattgcgt ttcatttaaa tcgctctgtg cggaagtaca
     2221 aaattcatgc tgtatatcgg caggattggc ggcaatgccg tggtaatggg cgaggatgat
     2281 gagggcggaa agggcgggga gcggtgcgga tacgatagac ataaataaaa tatagttaga
     2341 ttggatgtgg ataacggctg gctggaaaag gaatatatta agtagaaaga aatatataaa
     2401 taaaacagca gaacgcattg taaggatata tatgggaatt gtaaagagaa agtatggaaa
     2461 agttctcgtt tcaggaaggt aaaacggctt aggaatcgag ttagatgagg atgcctcgca
     2521 cctctcgtgc ctcctgcata ccgttaaggc acagggttaa ggtgcaggct gctccgaact
     2581 ctgttgcggt cgggtaatgt tattttttgt gtttcaggca gcctgaaata tctgtatatt
     2641 tttgttttaa atagatttta aagattgata actgttcttg acgatttttc aagaaaggag
     2701 taaatttcaa gaaaggagta aagtgactta ttatcaatga caagcaacgc gcgaagtgac
     2761 aaggaaaact atctacttaa attctaagga ggcttcgaat atcataaacc aatcagaaac
     2821 atagagataa aaattatgta caaatataat cctcttatac aatttattgc acagttgatt
     2881 atgtcttatg gagcaagcgt agggtgggca cttgctgccc cacgcgtttc atatttcaag
     2941 gcagcctgaa accgtgtggg cataaatgcc taccctacat cccaaaaaac aagcgcagcc
     3001 tgcgtgtgta gggtgcgaac tttcggcagg tagacacgca gttttatatt ttcaagctga
     3061 gggatgctta agaaaagtac aaaacattaa aaaataaggg gctgtactag attagcccta
     3121 aattccacac caatcccgca
//
LOCUS       DAWFJR010000147         2961 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWFJR010000147 DAWFJR010000000
VERSION     DAWFJR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.147"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            757..2880
                     /locus_tag="WGR04_002021"
     CDS             757..2880
                     /locus_tag="WGR04_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0795500.1"
                     /translation="MNTPLFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGFASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNDNGGKT
                     VPYSALDKRSYLAKIGTSFGDDDHRIVLSHMKDQHRGIRTVREEFAVSEKNSRITIKR
                     QAPAYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVKGPNH
                     TRITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNDQFESKEKETKKKEEDEK
                     KVHSYKLSNPTKTDAGVYVEAIQDIGDVTLTGGLRYDRFKVKTHDGKTVSSSNLNPSF
                     GVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGTKAERARNTEIGFNY
                     NDGTFAANGSYFWQTIKDALANPQNRHVSAAVREAVNAGYIKNHGYELGASYRTGGLT
                     AKVGVSHSKPRFYDTHPKNLLSANPEFGAQVGRTWTASLAYRFQNPNLEIGWRGRYVQ
                     KAVGSILVSGQKDRSGKLENVVRQGFGVNDIFANWKPLGKDTLNVNLSVNNVFNKFYY
                     PHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          793 a          841 c          672 g          655 t
ORIGIN      
        1 gacttagctc gaagagaacg attctctaag gtgctgaagc accgagtgag tcggttccgt
       61 actatccgta ctgtctgcgg cttcgccgcc ttgtcctgat ttccgttcgt tttcggttat
      121 tcccgataaa ttaccgccgt ttctcgtcat ttctttaacc cttcgtcatt cccgcgcagg
      181 cgggaatcca gtctgttcgg tttcggtttt tccgatagat tcctgccgcg ttaggagttc
      241 tagattcccg ctttcgcggg aatgacgcgg aaaggttgct tttcgtcatt cccgcgcagg
      301 cctcgtcatt cccgcgcagg cgggaatcca gtccgttcgt tttcggtttt ttccgataga
      361 ttcctgccac gttaggggtt ctagattccc gctttcgcgg gaatgacgcg gaaaggttgc
      421 ttttcgtcat tcccgcattt gttaatccac tatatttccg ccgtttttta tagtggacta
      481 acaaaaacca gtacagcgtt gcctcgcctt gccgtactat ctgtactgtc tgcggcttcg
      541 tcgccttatc ctgatttttg ttagtccact atacatttcc gacaaaacct gtcaacaaaa
      601 aacaacactt cgcaaataaa aacgataatc agctttgcaa aaatcccccc cccccctgtt
      661 aatataaata aaaataatta ttaattattt ttcttatcct gccaaatctt aacggtttgg
      721 atttacttcc cttcatacac tcaagaggac gattgaatga ataccccatt attccgtctc
      781 agcctgctct cgcttaccct ggcggcaggt tttgcccatg cggcagaaaa taatgccaat
      841 gtcgcattgg ataccgttac cgtaaaaggc gaccgccaag gcagcaaaat ccgtaccaac
      901 atcgttacgc ttcaacaaaa agacgaaagc accgcaaccg atatgcgcga actcttaaaa
      961 gaagagccct ccatcgattt cggcggcggc aacggcacgt cccaattcct gacgctgcgc
     1021 ggcatgggtc agaactctgt cgacatcaag gtggacaacg cctattccga cagccaaatc
     1081 ctttaccacc aaggcagatt tattgtcgat cccgctttgg ttaaagtcgt ttccgtacaa
     1141 aaaggcgcgg gtttcgcctc tgccggtatc ggcgcgacca acggcgcgat tatcgccaaa
     1201 accgtcgatg cccaagacct gctcaaaggc ttggataaaa actggggcgt gcgcctcaac
     1261 agcggctttg ccagcaacga aggcgtaagc tacggcgcaa gcgtattcgg aaaagagggc
     1321 aacttcgacg gtttgttctc ttacaaccgc aacgatgaaa aagattacga agccggcaaa
     1381 ggtttccgca atgacaacgg cggcaaaacc gtaccgtaca gcgcgctgga caaacgcagc
     1441 tacctcgcca aaatcggaac aagcttcggc gacgacgacc accgcatcgt attgagccac
     1501 atgaaagacc aacaccgagg catccgcact gtgcgtgaag agtttgccgt cagcgaaaaa
     1561 aattcacgga taactattaa acgccaagcc cctgcttacc gcgaaaccac tcaatccaac
     1621 accaacttgg cgtacaccgg caaagatttg ggctttgtcg aaaaactgga tgccaacgcc
     1681 tatgtgttgg aaaagaaacg ctattccgcc gatgacaaag ataacggcta cgcaggcaat
     1741 gtaaaaggcc ctaaccatac ccgaatcacc actcgaggta tgaatttcaa cttcgacagc
     1801 cgccttgccg aacaaaccct gttgaaatac ggtatcaact accgccatca ggaaatcaaa
     1861 ccgcaagcgt ttttgaacga ccaatttgaa agtaaagaaa aggaaactaa gaaaaaggaa
     1921 gaggatgaaa agaaagtcca ttcctacaaa ctgtccaacc cgaccaaaac cgatgccggc
     1981 gtatatgttg aagccatcca agacatcggc gatgttaccc tgaccggcgg gctgcgttac
     2041 gaccgcttca aggtaaaaac ccacgacggc aaaaccgttt caagcagcaa ccttaacccg
     2101 agtttcggcg tgatttggca gccgcacgaa cactggagct tcagcgcgag ccacaactac
     2161 gccagccgca gcccgcgcct gtatgacgcg ctgcaaaccc acggcaaacg cggcatcatc
     2221 tcgattgccg acggcaccaa agccgaacgc gcgcgcaata ccgaaatcgg tttcaactac
     2281 aacgacggca cgtttgccgc caacggcagc tacttctggc aaaccatcaa agacgcgctt
     2341 gccaatccgc aaaaccgcca cgtctctgcc gccgtccgtg aagccgtcaa cgccggttac
     2401 atcaaaaacc acggttacga attgggcgcg tcctaccgca ccggcggcct gactgccaaa
     2461 gtcggcgtaa gccacagcaa accgcgcttt tacgataccc atcctaaaaa cctgttgagc
     2521 gcgaaccctg aatttggcgc acaagtcggc cgcacttgga cggcttccct tgcctaccgc
     2581 ttccaaaatc cgaatctgga aatcggctgg cgcggacgct atgttcaaaa agccgtgggt
     2641 tcgatattgg tgtcaggtca aaaagaccgc agcggcaaat tggaaaacgt tgtacgccaa
     2701 ggtttcggtg tgaacgatat cttcgccaac tggaaaccgc tgggcaaaga cacgctcaat
     2761 gtcaatcttt cggttaacaa cgtgttcaac aagttctact atccgcacag ccaacgctgg
     2821 accaataccc tgccgggtgt gggccgcgat gtacgcttgg gcgtgaacta caagttctaa
     2881 aacgcatatc ccgagaaaat gccgtctgaa agcctttcag gcggcatctg ttctgataat
     2941 ttgatatata gtggattaac a
//
LOCUS       DAWFJR010000148         2846 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWFJR010000148 DAWFJR010000000
VERSION     DAWFJR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.148"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(53..529)
                     /gene="greA"
                     /locus_tag="WGR04_002022"
     CDS             complement(53..529)
                     /gene="greA"
                     /locus_tag="WGR04_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ0795501.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            921..2222
                     /gene="aroA"
                     /locus_tag="WGR04_002023"
     CDS             921..2222
                     /gene="aroA"
                     /locus_tag="WGR04_002023"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ0795502.1"
                     /translation="MTESVRLPVARLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLVNALRIAGADVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQSFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETAE"
     gene            complement(2265..2798)
                     /locus_tag="WGR04_002024"
     CDS             complement(2265..2798)
                     /locus_tag="WGR04_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0795503.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMATASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          596 a          782 c          775 g          693 t
ORIGIN      
        1 ttaatccact ataatttcct gcgtgtgtcg ggtgtatcga aatcaagccg aatcaaatat
       61 atcggacttc gataatgtcg tattcgcgca cgccgcccgg ggcttggact tccgccgtat
      121 cgccctcttc cttgccgatt aaggcgcggg caatcggtga gccgacatag attttgccct
      181 gtttgatatc cgcttcgtct tcgccgacaa tttggtaggt aacgtgttct tccgtttcca
      241 aatcttccag cgtaaccgtc gtaccgaaca cgattttgcc ttcggcgtgg atttcggtcg
      301 gattgatgat gtgggcaacg gaaagtttgt gttccagctc ggaaatgcgg ccttcgataa
      361 agccttggcg ttctttggcg gcttcatatt cggcgttttc ggacaaatcg ccgtgcgatc
      421 tggcttcagc aatcgcttcg atcacttcgg ggcgcgccac gcttttaagc tgctgcaatt
      481 cctgtttcag caattccgca ccgcgtacgg tcagggggat tttttgcatc ggtctttttt
      541 ctccatattc cggcacaccg gtttgcggca gcaaacatac cgcataccgt cttgttttgt
      601 gcgtccggat attaaaataa aaacacaagc cgcccggaaa atcggcggct tgtctgtcgt
      661 tgaacagcgg ctattctacc aaattctatg aaattggcaa tcgtgccgtg ccgtccgcaa
      721 acacgctatg tccgcaacaa aagccgaaaa tatgccggca aaaaaatttt agaaacaaaa
      781 aatttaaaaa taatcaattt tcggcacaaa aaaccatatt cacggacttt aaaaccgaaa
      841 atgccaaacc tgagattttt cagacggcat ttgcaccagt ataatgcagg ctgtttttat
      901 ctttatattt acgttttgcc atgaccgaat ccgtccgcct ccccgtcgcc cgtctcaaac
      961 cttccaccgt cgccctgccc ggctccaaaa gcatcagcaa ccgcaccctg ctgcttgccg
     1021 ccttgtccga caatgcttgc gaaatccatt ccctgctcaa atccgacgat accgaccgta
     1081 tgctcgaagc actcgataaa ctcggcgttg aaatcgaata tcttgccgaa gaccgtctga
     1141 aagtgcacgg cacgggcgga cgcttcccca accgcactgc cgatttgttt ttgggcaacg
     1201 cgggcacggc gttccgcccg ctgaccgccg cgcttgccgt tttgggcggc gattatcatc
     1261 tgcacggcgt gcctcgtatg cacgaacgcc ccatcggcga tttggtcaat gcgctgcgga
     1321 ttgccggggc tgatgtcgaa tatctcggca aggaacacta tccgccgctt catatcggcg
     1381 aacgccaaga caacggcgag cgcgtgattc cgattaaagg caatgtgtcc agccagtttc
     1441 tgaccgccct tttaatggcg ttgccgctga ccgggcaggc gtttgaaatc cgtatggtcg
     1501 gcgagttaat ttccaagccc tatatcgaca ttactttaaa actgatggcg caattcggcg
     1561 tacaggttat caatgaaggc taccgcgtct tcaaaattcc cgccgatgcg cactaccacg
     1621 cgcccgaaca cttgcacgtc gaaggcgatg cctctagcgc gtcctacttc ctcgcagccg
     1681 gtttgattgc cgccacgccc gtccgcgtta ccggtatcgg cgcaaacagc atacagggcg
     1741 atgtcgcctt tgcccgcgag ctggaaaaaa tcggggcgga cgtggtttgg ggcgaaaact
     1801 tcgtcgaagt ttcacgcccg aaggaacgtg ccgtccaatc ctttgatttg gatgcgaacc
     1861 atatccccga tgccgccatg accctcgcca tcgtcgcgct tgctacaggg caaacctgca
     1921 cgctgcgcaa catcggttcg tggcgcgtca aagaaaccga ccgcatcgcc gcgatggcaa
     1981 acgagttgcg caaactcggg gcaaaagtcg tcgaagaagc cgaagcaatt cacatcaccc
     2041 cgcccgaaac gctgacaccc gacgccgtca tcgacacgta cgacgaccac cgcatggcga
     2101 tgtgtttctc gctggtttcg ctgttgggcg tacccgtcgt catcaacgat ccgaaatgca
     2161 cccacaaaac cttcccgact tatttcgacg tgttctcatc gctgaccgaa acagcggaat
     2221 aaggcggcat tttgccgcga ttccggcgcg gcgcggcggg cggctcattc tgtaaaaaaa
     2281 gtatgtgcgc cgaggtagtt tttggcgtaa aacggtgtgg agagtttttc ggttttgatg
     2341 gttttgccgc tgctgggggc atggatgaat tcgccgttgc cgatatagag tccgacgtgt
     2401 gagtagcggt gtgcgccgcc ggtgttgaag aatacgaggt cgccggcctt aaggcggctg
     2461 tcggggattt tgcggcttgc cgtcgccatg tcgcgggcgg tgcgcggcag cttgacgttg
     2521 agggcgtttt tgtaaacgaa ttgaatcatg ccgctgcaat cgaagccggt tgcggtgctg
     2581 ctgccgcccc atttgtaggg cgtgccgatg agtccgaggc tgtggagcat gagttcctgc
     2641 gagccttgtg tgcggtcgat gtggctgatg cggacggctt ggatttgccg gactgtctgt
     2701 ttgggtttcg gttggcggtg tttgccggag gtcgtgccgc atgaggcgag gagcagtgcg
     2761 ctgagacaga ggaaaagggt tttgtcgggg ggaaacatgg tttttccttt gcgggttcgg
     2821 atatccgtct gaaggtgttt cagacg
//
LOCUS       DAWFJR010000149         2723 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWFJR010000149 DAWFJR010000000
VERSION     DAWFJR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.149"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..234
                     /locus_tag="WGR04_002025"
     CDS             <1..234
                     /locus_tag="WGR04_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ0795504.1"
                     /translation="LKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDGIIY
                     GYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            312..557
                     /locus_tag="WGR04_002026"
     CDS             312..557
                     /locus_tag="WGR04_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0795505.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            560..949
                     /locus_tag="WGR04_002027"
     CDS             560..949
                     /locus_tag="WGR04_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795506.1"
                     /translation="MWKIIKEDSDDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADLDEEIDNIYNIIGFVSSYSLSKLRRNALTGIAFLRGIDVYDPPVSKEK
                     ALKALEKYPEIYQKFQHFFPFVELPPL"
     gene            1072..2013
                     /gene="mafA"
                     /locus_tag="WGR04_002028"
     CDS             1072..2013
                     /gene="mafA"
                     /locus_tag="WGR04_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0795507.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTRNSGRKGERSAGLSVNGMGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWTGPYKVSKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            2017..>2723
                     /gene="mafB"
                     /locus_tag="WGR04_002029"
     CDS             2017..>2723
                     /gene="mafB"
                     /locus_tag="WGR04_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0795508.1"
                     /translation="MKPLRRLTNLLAACAVAAAAFGQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGQINVIQDYTHQMGNLLIQQAAIQGNLGYTVRFSGHG
                     YEEHAPFDNHAADSASKEQGSVDDGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRLSDNYNNLGSNFSDRADEANKKMFEHNAKL
                     DRWGNSMEFINGVAAGAL"
BASE COUNT          801 a          783 c          567 g          572 t
ORIGIN      
        1 ttaaaattag atttctactc tgaagttatt ataaaagatt cttgccctaa tgatttgtta
       61 gaaaatggtg agactattaa aggaaaaaaa ggagtggtat taggtataag cgaagaagat
      121 ggtataatct atggctatac tattttactt tttgatataa aatattgtat atatatagat
      181 aagaaatata ttataccgac tggaaaaaaa ttctctcgcg atgattttta ttagctaatt
      241 taagtaattt aagcaagttt tttagatact gaaactggta attatgttta tgtaagcata
      301 aggagttcag attgaaggat tctacagggc aaatgaggct ggcaaccaag gatttggcgg
      361 aagccattaa acgaggagaa gtacgtagtt ctgcttttac aacaaagcaa ctaaaggcaa
      421 tcgaaaaagg agaatctaaa ataccggatt acacttggca tcatcatcaa gacactggaa
      481 ggatgcaatt gattaatgaa gacttgcatc atgataccgg ccatatcggt tggagagcga
      541 tgagtaaagg aaagtaacta tgtggaaaat cataaaagag gatagtgatg atttaggatt
      601 tgcaattaaa tgcttattct ctcaatctat tgatttaaat gaattcaaat tatggattga
      661 acaagtaata cgcgatatgc ccattgagga catccctttt tatatttttg atttggcgga
      721 tttggatgaa gaaattgata atatttacaa tatcataggt tttgtttcga gttacagctt
      781 atcaaaattg agaagaaatg ccttaacagg cattgccttc ttaaggggga tagatgtcta
      841 tgaccctccc gtttcaaaag aaaaagcatt aaaagcctta gagaaatatc ctgaaattta
      901 tcagaaattt cagcatttct ttccgtttgt agagctgccg ccgctttaaa cagtcaaaat
      961 gccgtctgaa agccatttcc gccgctcaga cggcattttc gccccttttg tttacaaacc
     1021 cttaaaatcc ctttacactc aaaatccgtt caacatcaaa caaaccccgc tatgaaaacc
     1081 ctgctcctcc tcatccccct cgtcctcaca gcctgcggca cactgaccgg catacccgcc
     1141 cacggcggcg gcaaacgctt tgccgtcgaa caagaactcg tcgccgcatc gtcccgcgcc
     1201 gccgtcaaag aaatggactt gtccgccctg aaaggacgca aagccgccct ttacgtctcc
     1261 gttatgggcg accaaggttc gggcaacata agcggcggac gctactctat cgacgcactg
     1321 atacgcggcg gctaccacaa caaccccgaa agtgccaccc aatacagcta ccccgcctac
     1381 gacactaccg ccaccaccaa atccgacgcg ctctccagcg taaccacttc cacatcgctc
     1441 ttaaacgccc ccgccgccgc cctgactaga aacagcggac gcaaaggcga acgctccgcc
     1501 ggactgtccg tcaacggcat gggcgactac cgcaacgaaa ccctgctcgc caacccccgc
     1561 gacgtttcct tcctgaccaa cctcatccaa accgtcttct acctgcgcgg catcgaagtc
     1621 gtaccgcccg aatacgccga caccgacgta ttcgtaaccg tcgacgtatt cggcaccgtc
     1681 cgcagccgta ccgaactgca cctctacaac gccgaaaccc ttaaagccca aaccaagctc
     1741 gaatatttcg ccgtcgaccg cgacagccgg aaactgctga ttgcccctaa aaccgccgcc
     1801 tacgaatccc aataccaaga acaatacgcc ctttggaccg gcccttacaa agtcagcaaa
     1861 accgtcaaag cctcagaccg cctgatggtc gatttctccg acattacccc ctacggcgac
     1921 acaaccgccc aaaaccgtcc cgacttcaaa caaaacaacg gtaaaaaccc cgatgtcggc
     1981 aacgaagtca tccgccgccg caaaggagga taaaccgtga aaccgctgcg cagactgaca
     2041 aacctccttg ccgcctgcgc cgtagcggcc gccgccttcg gacagcccgc cctcgcagcg
     2101 gacttggcgc aagacccgtt cattaccgat aacgcccaac ggcagcacta cgaacccggc
     2161 ggcaaatacc acctcttcgg cgacccgcgc ggcagcgttt ccgaccgcac cggtcaaatc
     2221 aacgtcatcc aagactatac tcaccagatg ggcaacctgc tcatccaaca ggcggcaatc
     2281 caaggcaatc ttggttacac cgtccgcttt tcaggacacg gatacgaaga acacgccccc
     2341 ttcgacaacc acgccgccga cagcgcaagc aaagaacaag gcagcgttga cgacggcttt
     2401 accgtatacc ggctcaactg ggaaggacac gaacatcatc ccgccgatgc ctacgacggc
     2461 ccgaagggcg gcaattaccc caaacctacg ggggcacgcg acgaatacac ctatcacgtc
     2521 aacggcacag cccgcagtat caaactcaat ccgaccgaca cccgcagcat ccggcaacgc
     2581 ttgtccgaca actacaacaa tctcggcagc aatttctccg accgcgccga tgaagccaac
     2641 aaaaaaatgt tcgagcacaa tgccaagctc gaccgctggg gcaacagcat ggagtttatc
     2701 aacggcgtcg ccgccggcgc gct
//
LOCUS       DAWFJR010000150         2688 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWFJR010000150 DAWFJR010000000
VERSION     DAWFJR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.150"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            47..556
                     /locus_tag="WGR04_002030"
     CDS             47..556
                     /locus_tag="WGR04_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEZ0795509.1"
                     /translation="MPEIIRDLLEMPSETAVADIARTYSAHYLNPNNRNMTLFPDALP
                     CLDKLKAQGYWLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGLC
                     GELGLDPKEALVVGDTAHDLHMAANAGAAAVGVTTGAHSREQLLGAPHLAVLDGLSEL
                     PGFLAQHYA"
     gene            complement(644..1612)
                     /gene="ispH"
                     /locus_tag="WGR04_002031"
                     /gene_synonym="lytB"
     CDS             complement(644..1612)
                     /gene="ispH"
                     /locus_tag="WGR04_002031"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ0795510.1"
                     /translation="MNGKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLIYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     QEVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1642..2136)
                     /locus_tag="WGR04_002032"
     CDS             complement(1642..2136)
                     /locus_tag="WGR04_002032"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ0795511.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKD
                     GKKT"
     gene            complement(2434..2496)
                     /locus_tag="WGR04_002033"
                     /pseudo
     CDS             complement(2434..2496)
                     /locus_tag="WGR04_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          621 a          804 c          646 g          617 t
ORIGIN      
        1 ggatttcggt tgtcgtgagg gaaaggaatt ttgcaaaggt ctcagcctgc ccgaaatcat
       61 ccgcgacctg ctcgaaatgc cgtctgaaac cgccgttgcc gacatcgcgc gcacttattc
      121 cgcgcattac ctcaatccca acaaccgcaa tatgacgctc tttcccgatg ccctgccctg
      181 tctggacaag ctcaaagcac aaggatactg gcttgccgtc gccacgggca aagggcgggc
      241 gggtttggac aacgccatca gccaaaccgc caccggcggc tattggctcg ccaccgcctg
      301 cgcgggggaa tatccctcca aaccctcgcc cgaaatggta ttcggactct gcggcgaact
      361 gggactcgat ccgaaagaag cattggtcgt cggcgatacg gcgcacgacc tgcatatggc
      421 ggcaaacgca ggcgcggcgg cagtcggcgt taccaccggt gcacattcgc gcgaacagct
      481 ccttggcgca ccgcatctcg ccgtattgga cggtttgtcc gaactgcccg gttttcttgc
      541 acagcattac gcctgattgg tttccgcatc agacactcga aacaaatgcc gtctgaagcc
      601 tgttcagacg gcatttgtgt tgcccaaaca tccgatgcct gcgtcaacgt ttgcacaaat
      661 cgggtttggt ttcgccctcg cggcgcaact ctttgggcag gacgaacacg atgctttctt
      721 ccgcaccctc gccttcgcgt acagtttcgt gcccccatcc gcgtatggtt gccagcactt
      781 cctgcaccaa cacttcgggc gcggacgcgc ctgccgttac gccgactttg tttttgccct
      841 caaaccatgc acgttgcagg tagcctgcat tatccaccat atacgcatcg attccgcgcg
      901 atgccgccac ttcgcgcaag cggttgctgt tagacgaatt gggcgaaccg accacaatca
      961 cgatgtcgca ctgttctgcc aactctttga cggcggtttg ccggttggtc gtcgcatagc
     1021 agatatcttc cttgtgcgga ttgcggatat tggggaaacg cgcgttcagc gcggcgatga
     1081 tgtctttggt ttcatcgacc gagagcgtgg tttggctgac ataggcgagt ttgtcggggt
     1141 ttctgacttc gagttttgcc acatctccga ccgtttcgac caaaagcatt ttgcccggcg
     1201 caagctgtcc catcgttcct tcaacctcga cgtgcccctt atgcccgatc atgatgattt
     1261 cacagtcttg ggcatccagt cgggcgactt ccttatgcac tttcgtcacc agcgggcaag
     1321 tcgcatcaaa tacccggaaa ccacgttccg ccgcttcctg ctgcaccgcc ttcgatacgc
     1381 cgtgtgccga ataaatcagt gtcgcacccg gcggcacttc cgccaagtct tcgataaaca
     1441 ccgcgccttt ttcgcgcagg ttatcgacga cgaatttgtt gtggacgact tcgtggcgca
     1501 cataaatcgg cgcgccgaat tcttccaaag cacgttcgac aatactgatt gcccgatcca
     1561 caccggcgca gaagccgcgc ggattggcaa gaatgatggt ttttccgttc ataagttttg
     1621 cattccgtgt tcagacagca ttcacgtttt tttgccatct ttgcgatgga cgatattgtc
     1681 aagcacagcc aataccgcgc cgacgcagat aaaactgtcg gcaatattaa aggcgggata
     1741 aaaccaattt tgccaataaa acaataagaa atcgacgaca tgaccgtgta tcaggcgatc
     1801 gatgacattg cctaacgcac cgccgataat catcgccgca cccgttttgc cgagggttgc
     1861 aaactcgtcg cgcaagatgg cgcgtaccaa atacgcgctc accgccaccg ccagcaccaa
     1921 aaaaaagtat ttttgccagc cgccctgatc ggcaaggaag ctgaacgccg cacccgggtt
     1981 gtacaccagc gtcagatcga aaaaggaagg aatgacattg acgcgttccc gatactgaaa
     2041 cgacgacagc accgcccact tcgaccactg gtccagcacg atggcggcaa gtgccaatac
     2101 ccaatagcgc gttttacttg aaacagatga agacatattt ttcaacagcc ggtaaaagag
     2161 taccatttta cccgaaaacc ccctttcctg tacccgaaac ggcaaatgcc gtaatcttaa
     2221 aacccgtcat tcccgacaac accgtaatct cgaaacccgt cattcccgcg taggcgggaa
     2281 tccagacctg tccgcacaga aacttatcgg ataaaaacag ttgcccaaac cccgcgttct
     2341 atagtggatt aaattcaaac cagtacggca ttgcctcgcc ttagctcaaa gagaacgatt
     2401 ctctaaggtg ctgaagcacc gagtgaatcg gttccgtact atttgtactg tctgcggctt
     2461 cgttgccttg tcctgattta aatttaatcc actatagatt cccacttccg tgggaatgac
     2521 ggttcagttg cattccgaca acaccgtaat cttgaaatcc gtcattcccg cgcaggcggg
     2581 aatctatcgg aaatgactga aacctcgaga ttctagattc ccactttcgt gggaatgacg
     2641 gttcagttgc gttccaacaa caccgcaatc tcgaaatccg tcattccc
//
LOCUS       DAWFJR010000151         2501 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWFJR010000151 DAWFJR010000000
VERSION     DAWFJR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.151"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            520..1629
                     /locus_tag="WGR04_002034"
     CDS             520..1629
                     /locus_tag="WGR04_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0795512.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCITARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIVIGRDKLFIHPRLMELLRGEGNSFTSF"
BASE COUNT          740 a          586 c          542 g          633 t
ORIGIN      
        1 ttttttgagt ttcagtcatt cccgataaat tgccttagca ttgaatgtct agattcccgc
       61 cttcgcggga atgacgggat ttgaggtttc tgtttttgat tttctgtttt cgtaggaatg
      121 acggtttaaa agttacccga aacctcaaaa aaaaggctgt gttttaatat agtgttgata
      181 gacgtacttg gctgccatgt attcaatcgt ggaaatctat atcttcgtcc tcgccgaaat
      241 agtctatgcc cgatatacaa ttttgataca caaacttgga aatatgggta tcgtcgccgg
      301 agcgatagaa tgcggacagt tatatatata tatacggttt ttttagggaa gcgggcagat
      361 gaagggcgga ttttgaatta accccatgca aattgacttt tgcgggcggt tttacctccc
      421 atatatttac aaaagccaaa tttttaaaca tatatccttg atatatacac ggcgtaaaca
      481 tatactggaa acatctttaa attttccgaa attttaaata tgagcaactg gaaacccgat
      541 attccctata acgatttacc acccctgccg ccaaaacagg atattgaaag caaaaccatc
      601 ctgaaacgtt gtataaccgc ccgtgcatcc cttgcccgtt taaagcaggc ggcagaactg
      661 ataccgaatc aagccatgct gattaacacc cttcctgtta tggaagcccg tgcaagttcg
      721 gaaattgaaa acatcgtaac caccacggac aagctgtttc aatccctgca aatggatacg
      781 gaacggcaag accctgccac gaaagaagcc ctgcaatacc gcaccgccct gtttgcaggc
      841 tatgaatcac tgacgagccg ccctttatgc acacaaaccg ccatcatggt ctgcaacgcc
      901 atcaagcacc cctacgaaat ggccatccgc aaaacaggcg gcacagccct aaaaggaggc
      961 aacagcggaa atgttgtcta taccccgccc gaaggagaag aaaccatacg cggcaagctg
     1021 gcaaattggg agcggtttat tcacgaaagc ggcgatttag acccgcttat catcatggcg
     1081 gcggcacatt accaatttga agccatccat ccgtttacgg acggcaacgg gcggacgggg
     1141 cgcatattga acagcctgct attgattgaa aaagggcttt tggatttgcc tattttgtat
     1201 ttgagccgct acatcatcga aaacagggcg gactattacc gcctgctttt aggcgtaacc
     1261 gaacggcagg attgggaaag ctggataatc tacatcttag acggcgtagc cgacaccgcc
     1321 gattggacgg tatcgaaaat agatgcgata cgccgcctgt tcgagcagac acggcaacac
     1381 atacggacac acgcacaagg aatctacacg cacgaactgg taaatcttct gtttgagcag
     1441 ccatatacac gcattgccaa cctagaagcg gcagggatag ccaaacggca gacggcctct
     1501 aagtacctga aagagctttc agacataggt gtgctgcaag aaatcgtcat cggcagggac
     1561 aaactattca ttcatccgcg cctaatggaa ctattgcggg gagagggcaa cagctttact
     1621 tcattctaac cccctcttcc cccccacatg actaacacga aacagggatt ttgacacccg
     1681 aaccgagacc ccttgtattt cccccgcgaa aagccggcat ccgcccgcgt atcatgggag
     1741 caacaaaacc cctgcctaaa attttgactt gtgcaaattg ggggtatatt tggggatata
     1801 ttgaaaaatg gctaaaataa aatgtttaat aaacaaaatg ttgaaactta atttcgatag
     1861 agcatctgca tatcgtattg aggcgttcat ggaatttgag aaagctattt ttaaataaga
     1921 aaaggtaact attaaatagc tacctttcta aaattaaata tcaacacatc gttaaaacac
     1981 agcctttatt tttaacaaag ttgcaaatgt ttttttattt ttttagggaa tacaccaaaa
     2041 tcccgtcatt tccgcgcagt cgtgaatccg aacgcgtccg catggaaacc tatatcccgt
     2101 cattcccacg aaagtgggaa tctagttttt tgagtttcag tcattcccga taaattgcct
     2161 tagcattgaa tgtctagatt cccgcctgct cgggaataac gggatttgag gtttctgttt
     2221 ttgattttct gttttcgcgg gaatgacggt ttagaagtta cccgaaacct caaaaaaacc
     2281 gaaaccgaac ggactggatt cccgcctgcg cgggaatgac gggtttcgag attacagtgt
     2341 atcgagaagg atggaaaacg gtgaaaattg tgtaaaaaat gccgtctgaa gcctttcaga
     2401 cggcatttgc ggcattcgga catttaaaaa tcccatcatt cccgcgcagg agggaatccg
     2461 aatacatccg cacagaaacc tgcatcccgt cattcccacg a
//
LOCUS       DAWFJR010000152         2437 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWFJR010000152 DAWFJR010000000
VERSION     DAWFJR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.152"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(13..819)
                     /locus_tag="WGR04_002035"
     CDS             complement(13..819)
                     /locus_tag="WGR04_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ0795513.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRSF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1039..2409
                     /gene="ffh"
                     /locus_tag="WGR04_002036"
     CDS             1039..2409
                     /gene="ffh"
                     /locus_tag="WGR04_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ0795514.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLAGRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLHKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMRGIK
                     GMFPGL"
BASE COUNT          670 a          616 c          645 g          506 t
ORIGIN      
        1 tgttggctct tcttatcttt tcagaatgat ttccaatacg aacttgctgc ccatataggc
       61 aatcataagg ctgacaaatc cgatgatggt ccacacggcg gcttttttgc cgcgccatgc
      121 ggtcatgctg tgcttgagca gcagtccgcc gtaaatcagc catgacaata tgccgaatac
      181 ggttttatgg gtaaaggtca tgggtttgcc gaatacggct tcggcgaaga atgtgccgct
      241 gacgacggaa taggtcagca ggatgaaacc tgcccacatg gcctggaaca tgagtttttc
      301 caaactgagc agcgacggca ggaatcctgc gagcttggag aagctcctgc ggtgcaggct
      361 ccgattcagc agcagggtca aaacggacaa taatgttgcg atgccgaaca gcccgtatgc
      421 aagcagcgaa gttccgatat gcagcataaa gggaaggtcg gtaatttcat atcccgagaa
      481 ttttccagga aaaaccaaac ctgacagcag catcagtgcg gcgcaaggat acagcagcaa
      541 ctgcactccg cgcagcggat aaaagaagct gccggcaaaa taaataaaca gcatcatcca
      601 aacaatcagg ctgccggaat acccgaagcc cataatgatg attttgtctt gaatgaccgg
      661 cataagcagt gccgcgccgt ggacggtcaa tgccgcgccc aaaaccggca attccgtctt
      721 ccacgggtaa tcccggccgc gcccctgctg ttggcagtgc catgcaaatg cacccaatcc
      781 tgcgtaaacc gccgtcaaaa agatgaaaac tgtcggcatg gtggactttc tctatctata
      841 ctgttgcgcc gtctgcggcc gcttatgaaa tattggaact tttaacgttg gaattgtaaa
      901 atccccgttt cgggcaagcc ttgacggatt tgccgatatg ctgtccggca cacaagccgc
      961 atcaaattat tttgatttta ttttaacaaa gaatgcccct gatggggcaa gctattctta
     1021 ttcagaccaa ggaccagtat gttagacaat ttaaccggcc gcttcagcaa tgtcttcaaa
     1081 aacatccggg ggcaggccaa actgaccgaa gacaatatta aagaggcctt gcgcgaagtc
     1141 cgcctcgccc tgcttgaggc ggatgtcgcc ctgcctgtcg tcaaagagtt cgtcaacaac
     1201 gtcaaagaaa aggccctcgg tcaggaagtg gcgggcagcc tgacgccgga tcaggcattt
     1261 atcggcgtgg tcaacaaagc cctgaccgaa ctgatgggca gggaaaacaa aacgctggat
     1321 ttgtcggttg cgccgcccgc cgtcgtgttg atggcaggtt tgcagggcgc aggcaagacg
     1381 acgaccgtcg gcaaactcgc ccgcctgttg aaaaacgatc agaagaaaaa ggttttggtg
     1441 gtatccgccg acgtttaccg tcctgccgcg attgaacagc tgcgtctgtt ggccgaacag
     1501 gtcggcgtgg attttttccc gtccgatacc aaccaaaaac cggttgaaat tgcaactgcc
     1561 gccgtcgatt acgccaaaaa acatttttac gatgtattga tggtcgatac cgccggccgt
     1621 ttggcaatcg atgaagagat gatgaacgaa atcaaagccc ttcacgcggc ggttaacccg
     1681 gtggaaactt tgttcgtcat cgatgcgatg ctgggtcagg atgcggtgaa cactgctcag
     1741 gcatttaatg aagccctgcc gctgaccgga gtcgtattga ccaagatgga cggcgactcg
     1801 cgcggcggtg cggcattgtc cgtacgccac gtaaccggca aaccgattaa atttatcggt
     1861 gtcggcgaaa aaatcaacgg cctcgaacct ttccaccccg accgtcttgc cggccgcatt
     1921 ttgggtatgg gcgacgtatt gaccctgatt gaagacgttc aaaaaggtat agacgaagaa
     1981 gccgccgcta aaatggcgaa aaagctgcac aaaggcaaag gcttcgacct caacgacttt
     2041 aaagaacaaa tccagcaaat gcgcaatatg ggcggtttgg aaaacctgat gtcgaaaatg
     2101 ccgggcgaac tgggtcaaat ctcgaaacaa atccccgaag gaacggctga aaaagcgatg
     2161 ggcaaagtag aagccatcat caactcgatg acccctaaag aacgcgccaa ccctgccctg
     2221 ctcaaagcca gccgcaaacg ccgtattgca atgggtgcgg gcacaaccgt gcaggaagtg
     2281 aacaaattgc tcaaacagtt tgaacaaatg caacaaatga tgaagatgtt cagcggcaac
     2341 ggcttgggca aactgatgcg tatggcgaaa ggaatgaggg ggataaaagg gatgttcccg
     2401 ggtttgtaag ccgatttaac agaaaacgcc gtctgaa
//
LOCUS       DAWFJR010000153         2427 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWFJR010000153 DAWFJR010000000
VERSION     DAWFJR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.153"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            128..2341
                     /locus_tag="WGR04_002037"
     CDS             128..2341
                     /locus_tag="WGR04_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0795515.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPNNDKKLVLAARSLA
                     EAHYPADAATPYGEPLPDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          635 a          771 c          581 g          440 t
ORIGIN      
        1 aggaaaaatc agaaaagttt tgcattttga aaatgagatt gagcataaaa ttttagtaac
       61 ctatgttatt gcaaaggtct caagctgtta caataaactc gtttttatcg gaacggaaga
      121 ccccatcatg accgccatca gcccgattca agacacgcaa agcgcgactc tgcaagaatt
      181 gcgcgaatgg ttcgacagct actgcgccgc gctgccgaac aacgataaaa aacttgtctt
      241 agccgcccgt tcgctggcgg aagcacatta ccccgccgat gccgccacgc cgtatggcga
      301 gccgctgccc gaccacttcc tcggcgcggc gcaaatggtt catgaactcg acctgctccc
      361 cgatgccgtc gccgccaccc tgcttgccga catcggacgc tacgtccccg actggaacct
      421 attggtttcc gaacgctgca acagtaccgt cgccgagctg gtcaaaggtg tggacgaagt
      481 gcagaaactc acccacttcg cccgggtgga cagcctcgcc acaccggaag aacgcgccca
      541 gcaggcagaa actatgcgga aaatgctgct ggcgatggtt accgacatcc gcgtcgtgtt
      601 aatcaaactg gcgatgcgta cgcgcaccct gcaattttta agcaacgccc ccgacagccc
      661 cgaaaaacgc gccgtcgcca aagaaaccct cgacatcttc gccccgctcg ccaaccgttt
      721 gggcgtgtgg cagctcaaat ggcagctcga agatttgggc ttccgccatc aagagcccga
      781 aaaataccgc gaaattgccc tgcttttgga cgaaaaacgt accgaacgcc tcgaatacat
      841 cgaaaacttc cttaatatcc tgcgtacgga actcaaaaaa tacaatatcc actttgaagt
      901 cgccggccgt ccgaaacaca tctactccat ttacaaaaaa atggtgaaga aaaaactcag
      961 cttcgacggg ttgttcgaca tccgcgccgt gcggattctg gttgataccg tccccgagtg
     1021 ttacaccaca ctgggcattg tccacagcct ctggcagccc attcccggcg agttcgacga
     1081 ctacatcgcc aacccgaaag gcaacggcta taaaagtttg cacaccgtca tcgtcggccc
     1141 ggaagacaaa ggcgtggaag tgcaaatccg caccttcgat atgcaccaat tcaacgaatt
     1201 cggtgtcgcc gcgcactggc gttacaaaga gggcggcaaa ggcgattccg cctacgaaca
     1261 aaaaatcgcc tggttacgcc aacttttgga ctggcgcgaa aacatggcgg aaagcggcaa
     1321 ggaagacctc gccgccgcct tcaaaaccga gcttttcaac gacacgattt atgttttgac
     1381 cccgcacggc aaagtcctct ccctgcccac aggcgcgacc cccatcgact tcgcctacgc
     1441 cctgcacagc agcatcggcg accgttgccg cggtgcgaaa gtcgaagggc agattgtgcc
     1501 gctgtccacc ccgctcgaaa acggacagcg tgtcgaaatc attaccgcca aagaagggca
     1561 tccttccgtc aactggcttt acgaaggctg ggtcaaatcc aacaaggcaa tcggcaaaat
     1621 ccgcgcctac atccgccagc aaaacgccga caccgtgcgc gaagaaggcc gcgtccaact
     1681 cgacaaacag cttgccaaac tcacgcccaa acccaacctg caagagcttg ccgaaaatct
     1741 cggctacaaa aagccagaag acctctacac cgccgtcgga caaggcgaaa tttccaaccg
     1801 cgccatccaa aaagcctgcg gcacgctgaa cgaaccgccg cccgtacccg tcagcgaaac
     1861 caccatcgtc aaacagtcca aaatcaaaaa aggcggcaaa aacggcgtgc tcatcgacgg
     1921 cgaagacggt ctgatgacca cgcttgccaa atgctgcaaa cccgcgccgc ccgacgacat
     1981 tgtcggcttc gttacccgcg atcgcggcat ttcggtacac cgcaaaacct gcccctcttt
     2041 ccgacacctc gccgaacacg cgcccgaaaa agtactggac gcaagttggg cggcgttgca
     2101 ggaaggacaa gtgttcgccg tcgatatcga aatccgcgcc caagaccgct ccgggctttt
     2161 gcgcgacgta tccgacgcgc tcgcccgcca caaactcaac gttaccgccg tgcaaaccca
     2221 gtcccgcgac ttggaagcca gtatgcggtt cacgctcgaa gtcaaacagg tcaacgacct
     2281 cccgcgcgtc ctcgccagcc tcggcgacgt caaaggcgta ttgagcgtta cccggcttta
     2341 aatacaaaaa tgccgtctga aagccgtata acgcttcaga cggcattttg attgccgggg
     2401 tttgctattt tttgttgcat aatcaat
//
LOCUS       DAWFJR010000154         2346 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWFJR010000154 DAWFJR010000000
VERSION     DAWFJR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.154"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            145..531
                     /locus_tag="WGR04_002038"
     CDS             145..531
                     /locus_tag="WGR04_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ0795516.1"
                     /translation="MKGLDKYILFKNCETLGELFRIYQDYLSLNIDDPNLKIKFFDLL
                     EELLSDGVIELCDYRENPPKILTGSPKTQVDELRKIWPDMEEMLLYFPDNPWHYVEWF
                     WWGATCPIELTQLPKIEIYEEQMKQA"
     gene            580..981
                     /locus_tag="WGR04_002039"
                     /pseudo
     CDS             580..981
                     /locus_tag="WGR04_002039"
                     /inference="COORDINATES: protein motif:HMM:NF024728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
     gene            991..2181
                     /locus_tag="WGR04_002040"
     CDS             991..2181
                     /locus_tag="WGR04_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA"
                     /protein_id="HEZ0795517.1"
                     /translation="MTVGYGFSASGDYSQSKIRANHASVTEQSGIYAGEDGYQIKVGN
                     HTDLKGGIITSTQSAEDKGKNRFSTGTLAGSDIQNYSQYEGKSFGLGAGASISGKTLG
                     QGAQNKPQNKHLTSVADKNGASSSVGYGSDSDSQSSITKSGINTQNIQITDEAAQIRL
                     TGKTAAQTKADIDTNVTTDTAERHSGSLKNVFDKDRVQSELDLQRTVSQDFSKNVQQA
                     NTEINQHLDKLKADKEAAETAAAEALANGDMETAKHKAHEAQDAAAKADNWQQGKVIL
                     NMLASGLAAPTQSGAGIAAATASPAVSYAIGQHFKDLAGQNANGKLTASQETAHVLAH
                     AVLGAAVAAAGGNNAPAGALSAGGSEAAAPYISKWLYGKEKGSDLTAEEKETETFAKF
                     PKIP"
     gene            2212..>2346
                     /locus_tag="WGR04_002041"
     CDS             2212..>2346
                     /locus_tag="WGR04_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0795518.1"
                     /translation="MSTFFQQTAQAMIARHIDRFPLLKLDQVIDWQPIEPYLNRQRTR
                     Y"
BASE COUNT          821 a          545 c          532 g          448 t
ORIGIN      
        1 accttggaaa ggaacgacca tcattttaag gaatttttca actagccgag aacaaactgg
       61 agcaaaatgg acgattgagt ttcaaaatca tccattagta ggtaggaaaa aaatggaatt
      121 aaaatttaga taaggaattg aataatgaaa ggtttagata agtatatcct atttaaaaat
      181 tgtgaaacat taggagaatt gtttcgaata tatcaagact atctatcatt aaatattgat
      241 gacccaaatt taaaaattaa attttttgac cttttggaag aacttttatc agatggtgtt
      301 attgaattat gcgattatcg tgaaaatcct ccaaaaatat taaccggttc gccaaaaaca
      361 caggtagatg aattaagaaa aatttggccg gatatggaag agatgttgct atattttcca
      421 gataaccctt ggcattatgt tgaatggttt tggtggggag caacttgccc tattgaactg
      481 acacaattgc ctaaaataga aatttacgaa gaacaaatga aacaagctta aagccagcgt
      541 aaccagcctg tatgacaagg tgcaggtgaa caatccaata tccacattac cggctcagat
      601 gttgccggca aagaaggaac cggcctgatt gcagataagc aaatccatct gcaatcagcc
      661 gagcaaagta ataccgaacg cagccaaaac aaatcggcag gctggaacgc aggtgctgcc
      721 gtatcattcg gacaaggagg ctggtcatta ggcgttaccg caggcggcaa tatcggcaaa
      781 ggccacggca atggcgacag catcacccac cgccatagcc atatcggcga caaaggcagc
      841 caaaccctta tccaaagtgg tggcgatact accatcaaag gcgcgcaagt acgcggcaaa
      901 ggcgtacaag tcaatgccaa aaacctaagt attcaaagcg tacaagatag cgaaacctat
      961 caaagcaaac aacaaaacgc cagtgcacaa gtgaccgtcg gttatggctt cagtgccagt
     1021 ggcgattaca gccaaagcaa aatccgagcc aatcatgctt cggtaaccga gcaaagcggt
     1081 atttatgccg gagaagacgg ctatcaaatc aaggtcggaa accatacaga cctcaaaggc
     1141 ggcatcatca ccagtaccca aagcgcagaa gacaagggta aaaaccgatt cagcacaggc
     1201 acactcgccg gcagtgatat tcaaaattac agccaatacg aaggaaaaag ttttggattg
     1261 ggcgcaggtg cgtccataag cggcaaaaca ctgggacagg gcgcacaaaa taagcctcaa
     1321 aacaaacacc tgacaagcgt ggccgataaa aacggcgcaa gttcatcagt aggctacggc
     1381 agcgacagcg acagccaaag cagcatcaca aaaagcggca tcaataccca aaacattcaa
     1441 atcaccgacg aagccgcaca aatccggctg acaggaaaaa cagcggcaca aaccaaagcc
     1501 gatattgata caaacgtaac cacagacacc gccgaacgac attcgggcag cctgaaaaac
     1561 gtatttgaca aagatagagt gcaaagtgaa ctggatttac aaagaaccgt cagccaagat
     1621 tttagtaaaa atgttcaaca agccaatacc gagattaacc aacatttaga caaactcaaa
     1681 gcagacaaag aagcagccga aacagcagca gccgaggcat tagccaatgg cgatatggaa
     1741 actgccaaac acaaagccca tgaagctcaa gatgcggcag caaaagcaga taattggcaa
     1801 caaggcaaag tcattctcaa catgttagcc tcaggtttag ctgcgccgac ccaaagcgga
     1861 gcgggcatcg ctgcggctac cgcatcgcca gccgtatcgt atgcgattgg acagcacttt
     1921 aaagatttag ccggtcaaaa cgcgaatggc aaactaaccg ccagtcaaga aaccgctcac
     1981 gttcttgccc acgcggtatt aggagcagcg gttgcagcag caggaggcaa caatgccccg
     2041 gcgggagcat tgagtgcggg cgggtcggaa gcggctgcgc cttacatcag caaatggtta
     2101 tatggcaaag aaaaaggaag tgacttaacg gcggaagaga aagagactga gacctttgca
     2161 aaattcccca aaatccccta aattcccacc atgacattta ggggattttc catgagcacc
     2221 ttcttccagc aaaccgccca agccatgatt gccagacaca tcgaccgttt cccgctattg
     2281 aagttggacc aagtgattga ttggcagccg atcgaaccat acctgaaccg tcaaagaacc
     2341 cgttac
//
LOCUS       DAWFJR010000155         2313 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWFJR010000155 DAWFJR010000000
VERSION     DAWFJR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.155"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(59..1468)
                     /gene="dacB"
                     /locus_tag="WGR04_002042"
     CDS             complement(59..1468)
                     /gene="dacB"
                     /locus_tag="WGR04_002042"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ0795519.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGISDTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1632..2198
                     /locus_tag="WGR04_002043"
     CDS             1632..2198
                     /locus_tag="WGR04_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0795520.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          529 a          563 c          663 g          558 t
ORIGIN      
        1 agtttgtcct agttatctag gacagcccct taaatttaat ccactatatt ttcctgtttc
       61 aggctcggcg ttctttgcac atcagtttcg catccagcca gccatcgccg ccggagatga
      121 tgttgttggc aacgaagttg tccaagtctg gcagcaggga aacggcgcgg ccgctgttga
      181 tgatgacgac caccgccatc ggtttgtcgc ccagccaata acctgcaagg gcgcggacat
      241 tgttgagcgt gccggttttt aagcgcaaca gcccgccgct ttgtttgaag cggttgcgta
      301 aagtcccgtc tgtgccggcg atgggcagcg tgtcgatgaa atcttgtgca aacgggctga
      361 aataagccgt ttccaacatt tgcgccatca ttctcgccgt taccctttct tttctggaca
      421 gacctgaacc gttttccaaa accaaatccg caacatcgat gcccgacacg gcaagttcac
      481 gccggacggc agacgctgcc tgttcggaaa cggcgggcag tttgccgtcg ccgccgagtt
      541 tgaggaagac ggaacgcgca attagattgt ccgaacgctt gttcatgtcc gtcaaaattt
      601 ccttcatcgg ctttgagtgt gcaacggcaa gcgtctgcgc gccttccggc gtgtcggata
      661 tgccgatgcc gtctgaaatc cgtccgccgc cgagcagcca gtggttggta aaactttgcc
      721 ggatcagttc gtcaagcgcg aacatccgga caccgacagg cttgcccaaa cagctctcgg
      781 gaatattgcc gcgcaatttc agcgtattgt ccgaaaaaga tgcacgcatc agttttttga
      841 tcgaagggca ggcagcttgg gaggcggtaa ttttcaagtt gttttgggcg aaaatatgcg
      901 gcaaaggcgg atcggtgagg atgtcggtac tgtcggcggc attgcgttcg gcgcgcacca
      961 taaccatacc ggcagacagc atagttggat tggggggcgt cataaacggc gaaccgctgt
     1021 cggcttcgaa atcgtcgggg ctgccgactt cgccccacag gctgtggtcg agcatcaggt
     1081 gtcccgtgat attgcgtatg ccttgttcgc gcaactggcg ttggacggca agcaggtttt
     1141 cctgattgaa aacggggtcg ccgctgcccg cccaatatag gtttccgtca agcgtgccgt
     1201 cgtttaccgt accgttgctt ttaaactcgg tcgcccagcg gtaattgctg ccgaaggttt
     1261 tgaaggcggc aaacgcggta acgagtttca ttgtggaggc ggggttgacg gggacatccg
     1321 agcggtggtc aatgatgact tttccgctgt caagctcttg gacatatacg gcgatttcgt
     1381 tttgcggaat gcgacccgta tcgagcgcgt gtgcggcgag ggaagcgaga agcagcagca
     1441 gggaggccgc tgttttgggg aaattcatag gtaaatctgt tacataaaaa agcaattata
     1501 aggcaaagcc ggataagtgg gaacgctgtg gcggcggacc ggcttgtttg caggggaaat
     1561 cacatatata ataatcgcta tcattatgaa atgattgaga cgcacaaact taatattcag
     1621 gaggaatgat tatggctaag aaaatcagta ttttggtggg cagcctgcgc cgagcttctt
     1681 ttgcgcgcaa agtggcattg aatgcggcgg agatgttccc cgaaggctgg caggcggaaa
     1741 tcgtcgaaat cggacatctg ccgctttaca atttcgatta tgacgaccct gcggtggaag
     1801 atgtgccgct gcccgaaagc tacacggctt tccgcgaaac gattaaggct tcggacggca
     1861 ttttgttcgt tacgtccgaa aataaccgca ccattcccgc ctgtttgaaa aatgcggtgg
     1921 acatcggttc taaaccgaat gccgatgtgg cgtggaaaaa caaaccggcc ggcatcatca
     1981 gccattccgt cggcaagatg ggcggttaca gttcgcaaaa aaacctgcgc cttgcgctgt
     2041 cttatttcga tatgcccgta accggacagc cggaagtgtt tttgggcaat tcgccgacgc
     2101 tgtttgatga aaacggcaaa ttgatcgact cggcaaggga ttttgttcag tcatatatta
     2161 atcagtttgt cggtttgatt gaaagaaatg ccaaataaac aaacaagtcg aaaggccgca
     2221 aaaccggttc gggttttgcg gcttttttat atgggctggg cagcgtcaag gtttgccgtt
     2281 tgccggataa tgcgcggcaa atgaccggcg tgt
//
LOCUS       DAWFJR010000156         2265 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWFJR010000156 DAWFJR010000000
VERSION     DAWFJR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.156"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..574)
                     /locus_tag="WGR04_002044"
     CDS             complement(<1..574)
                     /locus_tag="WGR04_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ0795521.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRF"
     gene            737..2143
                     /gene="dnaB"
                     /locus_tag="WGR04_002045"
     CDS             737..2143
                     /gene="dnaB"
                     /locus_tag="WGR04_002045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ0795522.1"
                     /translation="MNDYAAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGRLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          542 a          585 c          612 g          526 t
ORIGIN      
        1 caaaacgttt ggcgacttcg tcccagttga cgatttccca gaaacctttc aggtagttgg
       61 gacggctgtt gcggtagtcg atgtagtagg cgtgttccca cacgtcgcag gtcagcagcg
      121 gcgtgttttc agtggtcagc ggcgtagcgg cgttggaagt ggaaaccaaa tccaatccgc
      181 cggcaggggt ttttaccagc cacgcccaac cggagccgaa agtaccggcc gcgcaggcat
      241 tgaacgcttc ttggaatttc tcgaagctgc cccatttcgc gtcgatggcg gcggccagtt
      301 cgccggaagg tttgccttga cctttgggcg tgaaacccag ccagtagaag gtgtggttcc
      361 aagtttgcgc cgcgttgttg aacacgccgc ccgaagattt tttcacaatc tcttccaaag
      421 gcaggttttc aaattcggtg cctttgattt gattgttcag gttggtgatg taggtttgat
      481 ggtgtttgcc gtagtggaat tccaaagtct ctttgctcag atgcggggac aatgcgtcca
      541 gttcataagg cagttgcggc agcttatgtt ccattttgtg ctcctaatat tgttttaaat
      601 gttgtatttt ggcagtgttg ctgcaaatag cttggcagcg cacatatttt acctgttttg
      661 aggaatggaa accaattaaa cctgctttac gctataatag aagattgcaa tttcggcacg
      721 acagatagga tgtaccatga acgattacgc agccatgccg tctgaagacc gtgaggtcgg
      781 cgcattgtcg cttccgccgc actcaatgga ggcggaacaa tccgttttgg gcgggttgat
      841 gctggaaaat ccggcatggg acaggattgc cgatgtggtt tcgggagagg atttctaccg
      901 gcatgaacac cgcctgattt tccgttccat tgccaaatta atcaatgaga gccgtcccgc
      961 agatgtgatt acggttcagg aagatttgca gcggaacgaa gaattggaag cggcaggcgg
     1021 attcgaatac ctgattacgc tggcgcaaaa caccccgtct gccgccaaca tccgccgcta
     1081 cgccgaaatc gtgcgcgagc gttccatcat gcgccaactc gccgaagtgg ggacggaaat
     1141 cgcccgcagc gcatacaatc cgcaaggcag ggacgcgggg cagcttttgg acgaggcgga
     1201 aaacaaagta ttccaaatcg ccgaaagcac cgccaaatcc aagcagggct ttttggagat
     1261 gcccgatttg ctgaaagaag tcgtacagcg catcgatatg ctctactcgc gcgacaatcc
     1321 cgatgaagtt accggcgtgc cgacggggtt catcgacctc gacaaaaaaa cctcgggtct
     1381 gcaacccggc gacctgatta tcgttgccgg tcgtccgtct atgggtaaga ccgccttttc
     1441 tatcaatatc gccgaacacg ttgccgtaga aggcaggctg cccgttgccg tcttttcgat
     1501 ggagatgggc ggtgcgcaac tggtcatgcg tatgctcggc tcggtcggac gcttggatca
     1561 aagcgtcctg aaaacaggca ggctcgaaga cgaacactgg ggtcgtctga atgaagcagt
     1621 cgtcaaactc tccgacgcgc ccgtgtacat cgacgagacc ccgggtctga ctgcgctcga
     1681 actgcgcgcc cgcgcccgtc gtctcgcccg tcaatttaac aataagctgg gattaatcgt
     1741 catcgactac ctgcaactga tggcgggatc cggccgttcc gacaaccgag cttcagagct
     1801 gggcgaaatt tcacgttcgc tcaaagcgtt ggcgaaagaa ttgcaagtcc ccatcatcgc
     1861 cctgtcgcaa ctgagccgca cggtcgaatc gcgtaccgac aaacgcccga tgatgtccga
     1921 ccttcgcgaa tccggtgcta tcgagcagga tgccgacctg attatgttta tgtaccgcga
     1981 cgaatactac aaccaagact cgcccatgaa aggccttgcc gaatgtatca tcggcaaaca
     2041 ccgcaacggt cccgtcggta aaatcttcct cacatggacg gggcaattca ccaaattcga
     2101 caatgctgcc tatattcccg aggaggcaaa gatagaggat taaatggcta tataaaaatt
     2161 tattaggcga aatcaggcaa aatcgtttaa aatcatgctt agagattgcc ctaaaaaata
     2221 aaacgcggtc ttgaggcatt tttgcattca gcccgcatat aattg
//
LOCUS       DAWFJR010000157         2099 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWFJR010000157 DAWFJR010000000
VERSION     DAWFJR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.157"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..1136)
                     /locus_tag="WGR04_002046"
     CDS             complement(<1..1136)
                     /locus_tag="WGR04_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795523.1"
                     /translation="MTSANFNINGFGDVKLTPYSPLLGYKAWDSFIGSIQSLSDLIYN
                     VDNNRNKMEITVNNAIQAADSFLSSIGRDNKITNTASLLASLDNIFLNLRNVSRDIRE
                     TGKFKPNDIQQAIGDIFIAAGDGLQYIKQQTEAMAQSKFLPTKLKTGLNDVLNSRMLK
                     SSTVLQHELNYLGFKIKDYGNERLGESIMNIDDFTPSKIANFFADPDTYSNVLEEVSR
                     FIYSLVPDDANPWKGGEDYIGRGISEWGELLEKWYKQDFLPYLEKEWDQFPKFEDWLP
                     EFPEWAREWLKLDPKRSGKYHVYDPLALDLDGDGIETVAAKGFAGALFDHRNQGIRTA
                     TGWVSADDGLLVRDLNGNGIIDNGAELFGDNTKLADGSFAKHGYA"
BASE COUNT          671 a          388 c          342 g          698 t
ORIGIN      
        1 gcgtagccgt gtttggcaaa agaaccgtct gccagtttgg tgttgtcgcc gaagagttcc
       61 gcaccgttgt cgatgatgcc gttgccgttc aaatcgcgca caagcagacc gtcatcggca
      121 gaaacccaac cggtggcggt gcggatgcct tgattgcggt ggtcgaacaa tgcacctgca
      181 aagcctttgg cagcgacagt ttctatgccg tcgccgtcca aatctagggc gagggggtcg
      241 tagacatgat atttgcctga acgtttggga tctaatttca accactctct tgcccattca
      301 gggaattcag gcagccaatc ttcaaatttc ggaaattggt cccattcttt ttcaagataa
      361 gggagaaaat cttgtttata ccatttttcc agtaactctc cccattcact tatccctcgt
      421 ccaatataat cttcgccccc tttccaaggg tttgcatcat caggaactaa ggaatatata
      481 aacctagata cttcttctaa tacattgctg tatgtatcag gatccgcaaa aaagtttgct
      541 atcttacttg gtgtaaaatc atctatattc attatagatt cgccaagcct ctcgtttcca
      601 taatccttta ttttaaatcc caaataattc aattcatgct gtaaaacagt agaggatttt
      661 agcattctag aattaaggac atcatttaaa ccagttttta atttagttgg taagaatttg
      721 ctttgagcca tcgcctctgt ttgttgtttt atatattgta atccatcacc agcagcaatg
      781 aatatatcac caattgcttg ttgaatatca ttaggtttaa attttcctgt ttctcgtata
      841 tctcgagata catttcttaa atttaaaaaa atgttatcga gggatgcaag taaagaagca
      901 gtatttgtta ttttgttatc tcttccaata ctgcttaaaa agctatctgc agcttggata
      961 gcattattaa cagtaatttc cattttattt ctattgttat ccacattata gattaaatca
     1021 gataaggatt gaatagaacc aataaatgaa tcccaagctt tatatcccaa gagtggtgaa
     1081 tagggtgtta atttcacatc tccaaaaccg ttaatattaa aatttgcaga tgtcatatct
     1141 gctttccttt caagtaaatt aaaaaaataa cgtgaggaat taaaattaag aataaattaa
     1201 ttatcgactc aatataatat gaatcaatta gctctcttat ggtaaatata agaggtaaaa
     1261 atattaaaaa taaactaata aagttcagaa aaatagtaaa tggaatattt tcatatggaa
     1321 aaattctaca aaatacaagt aatattccat gacatattag caaatgagta aaatttatta
     1381 taaaatatat aaatatttcc ccatcgtaag atatattcaa ataaagacat ataaacaata
     1441 tgaagtatat aaaatatatt ttttggcatc taaaaaataa acttttagca atcatatata
     1501 aatagcttgt agaataaaaa acgaaaatta ttaattatcg taggtgtgca tttattccaa
     1561 cacggtttca tgcagcttaa aaaccaaaaa ttctttctcc ctttatcctt tcttaaaaat
     1621 agcgttttca aattctgcaa cgacaggttt cattcaagta ggactgccgt gattgcatca
     1681 attgcttcgc ggcatggcat ccatatcaca caacaggaca tttattctcg ggtaagtcca
     1741 ccttcatcct cttccgccag caggaacagt tacaggctac ctaaaatatg ctctgtgctc
     1801 ggatagtaaa tatttccttt ctcactattg tttgtcattt aatcgacagt ctactctttc
     1861 tctaaaattt cgtcaataat agttaaatac ttttatttat tttaaataat gatatacatt
     1921 tatggttatt tgtttagaca gcctaaaaca aacggggaaa tcgtgttttc ccgaacatgg
     1981 ctaatggata gcattttgtt cgtgaagcgg ctgtgcagat tgtcggctgc taaaagtaaa
     2041 tcccccattt ttgcggttgt accgtttgtt ttggtatagc tgccttgctg agccaaagt
//
LOCUS       DAWFJR010000158         2096 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWFJR010000158 DAWFJR010000000
VERSION     DAWFJR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.158"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..443
                     /locus_tag="WGR04_002047"
     CDS             <1..443
                     /locus_tag="WGR04_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0795524.1"
                     /translation="ENNGNREDLKTENQENGTFHASSSLGLSAIYDFKLNDKFKPYIG
                     ARVAYGHVRHSIDSTKKTTDVVTVATNTPDGAPTIYNVPKTQNAYHESHSIRRLGLGV
                     VAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYRF"
BASE COUNT          533 a          626 c          490 g          447 t
ORIGIN      
        1 aagaaaacaa tggcaacagg gaagacctga agacggaaaa tcaggaaaac ggtacgttcc
       61 acgcctcttc ttctctcggc ttatccgcca tttacgattt caaactcaac gataaattca
      121 aaccctatat cggtgcgcgc gtcgcctacg gacacgtcag acacagcatc gattcgacca
      181 aaaaaacaac agatgttgtt accgtcgcca ccaatactcc tgacggagca cctacgattt
      241 ataatgtgcc aaagacgcaa aacgcctatc acgaaagcca cagcatccgc cgcttaggtc
      301 ttggtgtcgt cgccggtgtc ggtttcgaca tcacgcccaa gctgacttta gacaccggat
      361 accgctacca caactgggga cgcttggaaa acacccgctt caaaacccac gaagtctcat
      421 tgggcatgcg ctaccgcttc tgattccccg ataccgatgc cgtctgaacc ttcagacggc
      481 atttttgatt cacctgccgt ttacagacgc ggggcgggcg tggaaatacc caaaccgtca
      541 ttcccgacaa caccgcaatc ttgaaacccg ccattcccga taacagcgca atcttgaaac
      601 ccgtcattcc cgcgcaggcg ggaatccagc cccctgacgc ggcgggaatc tatcggaaat
      661 gactgaaacc ccgagattct agattcccac ttccgtggga atgacggttc agttgcgttc
      721 cgacaacacc gtaatctcga aacccgtcat tcccgataac agcgcaatct tgaaacccgt
      781 cattcccgac aacaccgtaa tctcgaaatt cgtcattccc gcgcaggcgg gaatccagcc
      841 ccctgacgcg gcgggaatct atcggaaatg actgaaaccc ccgagattct agattcccac
      901 ttccgtggga atgacgtggt gcaggtttcc gtatggatgg attcgtcatt cccgacaaca
      961 ccgtaatctc gaaatccgtc attcccgcac aggcgggaat ccagaccccc gacgcggcgg
     1021 gaatctatcg gaaatgactg aaaccccgag attctagatt cccacttccg tgggaatgac
     1081 ggttcagttg cgttccgaca acaccgcaat cttgaaattc gtcattcccg cgcaggcggg
     1141 aatccagacc cctgacgcgg cgggaatcta tcggaaatga ctgaaacccc gagattctag
     1201 attcccactt ccgtgggaat gacgattcag ttgcgttccg acaacaccgc aatctcgaaa
     1261 cccgtcattc ccgcgcaggc gggaatccag acccctgacg cggcgggaat ctatcggaaa
     1321 tgactgaaac cccgagattc tagattccca cttccgtggg aatgacgtgg tgcaggttcc
     1381 gtatggatgg attcgtctcc cgcacaggcg ggaatccaga cctgtccgca cagaaactta
     1441 tcggataaaa acagttgccc aaaccccgcg ttctagattc ccacttccgt gggaatgacg
     1501 gttcagttgc gttccgacaa caccgtaatc ttaaaacccg tcattcccgc gcaggcggga
     1561 atctagacct ccgacgcggc gggaatctat cggaaatgac tgaacccccg agattctaga
     1621 ttcccactgt cgtgggaatg acggttcagt tgcgttccga caacaccgta atcttaaaac
     1681 ccgtcatttc cgacaacgcc gcaatcttga aattcgtcat tcccgcacag gcgggaatcc
     1741 agacccccga cgcggcggga atctatcgga aatgactgaa accccgagat tctagattcc
     1801 cacttccgtg ggaatgacgt ggtgcaggtt tccgtatgga tggatccgtc attcccgcgc
     1861 aggcgggaat ctatcggaaa tgactgaaac cccgagattc tagattccca ctttcgtggg
     1921 aatgacggtt cagttgcgtt ccgacaacac cgtaatcttg aaacccgtca tttccgacaa
     1981 cgccgcaatc ttgaaattcg tcattcccgc gcaggcggga atctaggtct gtcggtgcgg
     2041 aaacgtatcg gataaaacgg tttcttcaga ttttacgttc tggattccca ctttcg
//
LOCUS       DAWFJR010000159         1998 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWFJR010000159 DAWFJR010000000
VERSION     DAWFJR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.159"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            298..1755
                     /locus_tag="WGR04_002048"
     CDS             298..1755
                     /locus_tag="WGR04_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0795525.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIVYFTRLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          450 a          597 c          463 g          488 t
ORIGIN      
        1 gcgcccttcg gcgtggcgga tatataagga agtgattttc catctaagca aaaaccgccc
       61 tatcggataa gcccttaaca gaaaaggctt tacccgcgcc gtatcggaac acatcctcta
      121 aaatacaatc cgttgaattg aaaaaaatat aaaaacatcc gcccgcgaaa aacggcagcg
      181 cgtcgtttga caaagaatga aaatatcggt taaaaaccga ttttcataca aaaaacaccg
      241 ctgccgtccg catccgtttc agacggtatt gagagaaaat cttttaggag aacctttatg
      301 tcccggcatc ccgcccccac cggagaaaaa acattcttcg gccacccctt ccagctttcc
      361 accctcttcc atatcgaatt gtgggaacgt ttttcatttt acggaatgca gggcatcctg
      421 ctgatttacc tctactacac cgccgacaaa ggcggcttgg gcatagacaa aaccctcgcc
      481 ggcggcattg tcggcgcata cagcggcagc gtgtacctgt ccaccatttt gggggcgtgg
      541 tttgccgacc gagtatgggg tgcggaaaaa accctcttcc tctcgggcat cgtcgtgatg
      601 ctcggacaca tcgtccttgc cgccgccccg ggcctgtacg gccttttaat cgggctgata
      661 ttcatcgcat tgggcagcgg cggcgtgaaa tctacggcca gttctatggt gggcgcatta
      721 tacgaacaag acgaaatgcg cccgctgcgc gatgcgggat tttccatttt ctacatcgcc
      781 atcaatatcg gcggcttcct gggcccgctg ctgaccggcc tgctgcagga aaacatcggt
      841 ttccattatg gtttcggcgc ggcggcggtc ggtatggcat tcggcttgtg gcgttattcc
      901 ctgggacgta aaaacctgcc ccaccccacc gtcccccatc cgctttcaaa aggacagggc
      961 aaaactgcgg ccgccgtcgg catcgccctc atcgccgcac ttgcaaccgc catcaaaacc
     1021 gggcttgtca acctcgacaa tttctccggc atcctcctat ctaccgtcat ccttgccgtc
     1081 atcgtctatt ttacccggct gctgaccaac ccccgcgtca gttccgacaa caagcggcac
     1141 atcatcgcct acatcccgct tttcctgacc atctgtatgt tttgggcggt ctggtttcag
     1201 atttacaccg tggcaaccgt ctatttcgac gaaaccgtca accgcaccat cggttcgttt
     1261 accgtgcccg tcgcctggaa agattccatg caaagcctgt gggtcatcct gttttccgga
     1321 ctgatggcgg caatgtggac aaaaatgggg cgcaaacagc ccaaaacccc gctgaaattc
     1381 gctatggcgg tatttgttac cggcgcgtcg tttttgggat tcgtcccctt tatttccgcc
     1441 ggcacgccga tgcctattgc ggttttcgca ctgattgtcc ttgccatcac gataggcgaa
     1501 ctgatgattt ccccgattgc gctgtccatc tccaccaaaa tcgcaccgcc tttattcaaa
     1561 acccaaatgg tcgcccttaa tttccttgcc ttttcattag gcttcacttt gggcggcgta
     1621 ttgtttgaaa aaggctatca ggcgggcgac gaaatcggct tctatcggct gctgttctac
     1681 atcggcgcag ccacaggctt cctgctgctc ctgctcgtcc ccaaattgaa caaaatgctc
     1741 gaaggcacag actaagtccc gccccgatgc cgtctgaacc cttcagacgg catttttccg
     1801 cataatgaaa ccaaaccgtt tccacccgac aggacaggct ccccccaacc ggaaggcagc
     1861 ctgccgattg tcatttaaat atctgactga ttccgtctgt ttgcaaggga aatagccgtt
     1921 tatttccgct tgggcggaaa ccggttctat tgagtaaaag gcattttatc cgactaaatt
     1981 agtgctgcat caacggaa
//
LOCUS       DAWFJR010000160         1962 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWFJR010000160 DAWFJR010000000
VERSION     DAWFJR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.160"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            584..1888
                     /locus_tag="WGR04_002049"
     CDS             584..1888
                     /locus_tag="WGR04_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ0795526.1"
                     /translation="MKKHIWAASLLPTSLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          411 a          486 c          565 g          500 t
ORIGIN      
        1 gcgggaatga cggttcgggc attccttaaa tcacccgtgt atcgctgtaa atcttagaga
       61 tggcggaata tagcggatta acaaaaacca gtacggcgtt gtcgcgcctt agctcaaaga
      121 gaacgattct ctaaggtgct gaagcaccga gtgaatcggt tccgtactat tcgtactgtc
      181 tgcggcttcg tcgccttgtc ctgatttttg ttaatccact atatttccaa ttaaaaattt
      241 ttaatatatt aatcaataaa ttaattttat aaaataaaaa tattgtcaac aatattttgc
      301 cttatcgccc aaacctctgt atattttcct acagtaaatt gttgacaatc catacgccca
      361 catatgcgcc gcctaaggat aaatcctccc gccggacaac gggtgcaagg gattggatgc
      421 gatatttcca tattcaaaca agggatttgt ttcacgcaca gggcggcaca tcggcaaaat
      481 ccccgcgccg cgccggtccg gcagggcttg cgtccctccc ggacaagccc cgaccccgcc
      541 tttccgaaag acgggctcaa ccattaagga aactttaatc aaaatgaaaa aacacatatg
      601 ggcggcatct ttgctgccga catccctatc ggcagaacct ttaaactggt ggaagcctta
      661 ttccgccgtc aattcgggcg ataccgcctg ggtgatgact gcggctgcct tggtactgtt
      721 gatgacgctt cccgggctgg ctttattcta cggcggtatg gtgcggaaaa aaaacctgct
      781 ctcgacgatg atgcacagct tttccatcgc gacattggtg ggcatccttt gggtcgccgt
      841 cggctattct ttagcgttca cgccgggaaa tgcctttatc ggcggtttgg ggcgcgtatt
      901 tttaagcggg atgcagatag acgctgccgc acagatgctg accgtgtcgc ccaatgcgcc
      961 gactgttccc gaaccggtat ttatgttttt tcagatgacg tttgccatta tttcgaccgc
     1021 cattattacc ggcgcgtttg ccgaacggat gaaatattcg gcaatgatgc tgttttcggg
     1081 catatggttt ttattggttt atgtgccggg cgcgcattgg gtgtggggcg gcggctttat
     1141 gagcaagggc ggcgtattgg attatgccgg cggtacggtg gtgcacatca atgccggtat
     1201 cgcgggactc gtcgccgcct tggttttggg caggcgcata ggctacgggc gcgaggcgat
     1261 gcctccgcac aatatggcga tgacactgat cggcgcggca atgttgtggt tcggctggtt
     1321 cggctttaac gccggatccg cacttgcggc agacgcggcg gcgggtatgg cgatggcggt
     1381 aacgcaggtg tcggccgtat tcggcgcggc aggctggctt gcctgcgaaa aaatagcggg
     1441 acacaaacct tccgctttgg ggctggcttc cggcgcggtt tccggtctgg tcggcatcac
     1501 ccctgccgcc ggctttaccg gcccgtcggg cgcggccgcc atcggtatat tgactgccgc
     1561 cgcgtgcttt gtgtccgtca ccgtcgtcaa acacaaattg cgttacgatg attctttgga
     1621 cgctttcggc atacacggat tcggcgggct ggtgggcgga atattgaccg gcatcttttt
     1681 cgacaaccgc attttcggcg gggatgcggc agtttggcag cagttgtgga tacaggtaaa
     1741 agacgggttc gttatggcgg catacagcgg gctgatgagt tgggcgattt tgaaggtcgt
     1801 ggggaaaatc tgcggcggcc tgcgcgtcgg caaggatgtc gaacgcgaag gtttggatct
     1861 gaatatccac ggcgaacgcg tggaataagg gcggttatgc cgtctgaagc ctgaaaatcg
     1921 ggtttcagac ggcatttttc acgtttgccg ccgatggata aa
//
LOCUS       DAWFJR010000161         1959 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWFJR010000161 DAWFJR010000000
VERSION     DAWFJR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.161"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..867
                     /gene="rfbB"
                     /locus_tag="WGR04_002050"
     CDS             <1..867
                     /gene="rfbB"
                     /locus_tag="WGR04_002050"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ0795527.1"
                     /translation="RVFAQHRPDAVMHLAAESHVDRSIGSAGEFIQTNIVGTFNLLEA
                     ARAYRQQMPSEKREAFRFHHISTDEVYGDLHGTDDLFTETAPYAPSSPYSASKASSDH
                     LVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGDGMQIRDWL
                     FVEDHARALYQVVTEGVAGETYNIGGHNEKANLEVIKTICALLEELVPEKPAGVARYE
                     DLITFVQDRPGHDVRYAIDTAKISRELGWKPQETFESGLHKTVQWYLDNKTWWQNVLN
                     GSYRLERLGTGK"
     gene            930..1796
                     /gene="rfbA"
                     /locus_tag="WGR04_002051"
     CDS             930..1796
                     /gene="rfbA"
                     /locus_tag="WGR04_002051"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ0795528.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     RIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEITDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            1835..>1959
                     /locus_tag="WGR04_002052"
     CDS             1835..>1959
                     /locus_tag="WGR04_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEZ0795529.1"
                     /translation="MKDKKMNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKT"
BASE COUNT          501 a          544 c          484 g          430 t
ORIGIN      
        1 cgcgtattcg cacaacaccg gcccgatgcc gtgatgcact tggcggcgga aagccatgtc
       61 gaccgctcta tcggttcggc aggcgagttt atccaaacca atatcgtcgg cacattcaac
      121 ctacttgaag ctgcgcgtgc ctaccggcag caaatgccgt ctgaaaagcg cgaagctttc
      181 cgttttcatc atatttcgac tgatgaagtg tacggcgatt tgcacggcac ggatgatttg
      241 ttcaccgaaa ccgcgcccta cgcgccgtcc agcccctact ctgcctctaa agcgtccagc
      301 gaccacctcg tccgcgcgtg gttgcgtact tacggcttgc cgaccattgt aaccaactgc
      361 tccaacaact acggtcctta ccattttccg gaaaaactca ttcctttgat gattctgaac
      421 gcgcttgacg gcaaaccgct gcctgtgtac ggcgacggta tgcaaatccg cgactggctg
      481 tttgtcgaag accacgcgcg cgcactgtat caggttgtta ccgaaggtgt tgccggcgaa
      541 acctacaata tcggcggaca caatgaaaaa gccaatctcg aagtaatcaa aaccatctgc
      601 gccctgctgg aagagcttgt tcctgaaaaa ccggccggtg tggcgcgtta tgaagatttg
      661 attactttcg tacaagaccg ccccggccat gacgtacgct acgccattga tacagccaaa
      721 atcagtaggg aactaggctg gaaaccgcag gaaaccttcg agtccggcct ccacaaaacc
      781 gtgcaatggt atctggacaa caaaacctgg tggcaaaacg tattgaacgg cagctatcgt
      841 ttggaacgct taggtacagg caaatagttt tcagacggca tcccgacgca atgccgtctg
      901 aaaacccatc gcaaaggaag aaagaaaaga tgaaaggcat catactggca ggcggcagcg
      961 gcacgcgcct ctaccccatc acgcgcggcg tatccaaaca gctcctgccc gtgtacgaca
     1021 aaccgatgat ttattacccc ttgtcggttt tgatgctggc aagaatccgc gatattttgg
     1081 tgattaccgc gcctgaagac aacgcctctt tcaaacgcct gcttggcgac ggcagcgatt
     1141 tcggcatttc catcagttat gccgtgcaac ccagtccgga cggcttggca caggcattta
     1201 tcatcggcga agaatttatc ggcaacgaca atgtttgctt ggttttgggc gacaatattt
     1261 tttacggtca gtcgtttacg caaacattga aacaggcggc agcgcaaacg cacggcgcaa
     1321 ccgtgtttgc ttatcaggtc aaaaaccccg aacgtttcgg cgtggttgaa tttaacgaaa
     1381 acttccgcgc cgtttccatc gaagaaaaac cgcaacggcc caaatccgat tgggcggtaa
     1441 ccggcttgta tttctacgac aaccgcgccg tcgagttcgc caaacagctc aaaccgtccg
     1501 cacgcggcga attggaaatt accgacctca accggatgta tttggaagac ggctcgctct
     1561 ccgttcaaat attgggacgc ggtttcgcgt ggctggacac cggtacgcac gaaagcctgc
     1621 acgaggcggc ttcgttcgtc caaaccgtgc aaaacatcca aaacctgcac atcgcctgcc
     1681 tggaagaaat cgcttggcgc aacggttggc tttccgatga aaaactggaa gaattggcgc
     1741 gcccgatggc gaaaaaccaa tacggccaat atttgctgcg cctgttgaaa aaataatgtt
     1801 tgaggccgtc tgaaactttt cagacggcct ttagatgaaa gataaaaaga tgaacatcat
     1861 tgataccgcc attcctgacg taaaactgct tgagccccaa gtcttcggcg acgcgcgcgg
     1921 cttttttatg gaaaccttcc gcgacgagtg gtttaaaac
//
LOCUS       DAWFJR010000162         1952 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWFJR010000162 DAWFJR010000000
VERSION     DAWFJR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.162"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            257..>1952
                     /locus_tag="WGR04_002053"
     CDS             257..>1952
                     /locus_tag="WGR04_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ0795530.1"
                     /translation="MNKTWKRQVFRHTALYTAILMFSHTGGGGGQAQAQTQTHKYAIV
                     MNGQKLPEVKWGDDYRNLAQKSNERQFTHTSGFGIKKNVTLSFNNTDEVVAQKNGTVV
                     FGAATYLPPYGKVSGFDTAKLTERGKAVDWIRTTHPGLIGYSYENVTCSNNYHNASSG
                     CPELSYKTQFTFGNQGLKRKTNGRLDIDEDKSRDGSPIYKLQNYPWLGVSFNLSSESL
                     VKLIKHNKIISSFSEDVTQSNGTNSQYKDKNLVYTTGDYQYKNKYSSRYVGQDEHSAV
                     AFYLNAKLHLLDKKQIQNIAQVSELNLGTLRTLIEPTEEWKKKRTNFFQGGNWTFEDK
                     GKVSVKLKLPQVKAGRCINKPNPNPNKKDLSPALTAPALWFGPVQNGKAEMYSASVST
                     YPDSSSSRIFLQNLKRKNDPNKPGRYSLADLTKSDIESRQPGFTGRQTVIRLDGGVQQ
                     IKLDQNKEATGLNGNTGKNDTFGIVSEGSFTPDVSEWKKVLLPWTVRGFSNDNEFVKF
                     NKESNKYSQRYRIRENGNNGKRNLGDIVNSPIVAVGGYLATSANDGMVHIFKKGNGD"
BASE COUNT          589 a          466 c          482 g          415 t
ORIGIN      
        1 gcttgttcgg tttcggtttt tttgaggttt cgggcaactt ctaaaccgtc attcccgcgt
       61 aggcgggaat ccagaccgtt gggcatctgc agcggtttgc taaaaaacgc tttaccgtga
      121 tcagtgtgca aagttaaaat ggggaagtaa gcttttcaat cagcaattcg gcgggcgcgg
      181 aatcgggcgg tttaccgaac ctcggcgttc gcggcgcgcc gccccgtccc gtgaaggcaa
      241 actcaaggaa taaaagatga ataaaacttg gaaacggcag gttttccgcc ataccgcgct
      301 ttataccgcc atattgatgt tttcccatac cggcgggggg ggggggcagg cgcaagcgca
      361 aacgcaaacg cataaatacg ctattgtaat gaacggacaa aagctgcccg aggtaaagtg
      421 gggggatgac tatagaaatt tggcgcaaaa aagcaatgaa cgccaattta cccatacgag
      481 tggtttcggc atcaaaaaaa acgtaacttt atcattcaat aataccgatg aagttgttgc
      541 tcaaaaaaac ggtactgtcg ttttcggcgc ggcgacctac ctgccgccct acggcaaggt
      601 ttccggtttt gataccgcta agctgaccga gcgcggcaaa gccgttgatt ggattcgtac
      661 gacccacccg gggttgatag gctacagcta cgaaaatgtc acatgcagca ataactacca
      721 caacgccagc agcggctgtc ctgaacttag ctataaaacc caatttacct ttggtaatca
      781 agggttgaaa agaaagacaa acggcaggct ggatatagac gaagacaaaa gccgcgacgg
      841 ttcgcccatt tacaaattgc agaattatcc ctggttgggc gtgtctttca atttgagcag
      901 cgagagcctc gtcaaattaa taaaacacaa taaaattata tcttctttta gcgaggacgt
      961 gacgcagagc aatggtacga acagccaata caaagacaaa aacctcgttt atacgacagg
     1021 cgattaccag tataagaata aatattcttc ccgttatgtc gggcaggacg aacactccgc
     1081 cgtcgccttt tatctgaacg ccaaactgca cctgctggat aaaaaacaaa tccaaaatat
     1141 cgcgcaagtc agtgaactta atttgggtac gttgagaacg ctcatcgagc cgacggaaga
     1201 atggaaaaaa aagaggacga acttttttca aggtggtaat tggacgtttg aagataaagg
     1261 aaaagtcagc gtcaaactca aattgccgca agtcaaagca ggccgctgca tcaacaaacc
     1321 gaaccccaat cccaacaaaa aagacctttc gccggcactg actgcccccg cgctgtggtt
     1381 cggacctgtg caaaatggta aggcggagat gtattccgct tcggtttcca cctaccccga
     1441 cagttcgagc agccgcatct tccttcaaaa tctgaaaaga aaaaacgacc ccaacaaacc
     1501 cggccgctat tctctcgcag acttgactaa gtcggatatt gaaagtcgac agccaggttt
     1561 cacagggcgg caaaccgtca tccgattgga tggtggcgtg cagcagatca aactggatca
     1621 aaacaaagag gccaccggtt tgaatggaaa caccggcaaa aacgatactt tcggcattgt
     1681 tagtgaaggg agcttcacgc ctgatgtcag cgagtggaaa aaagtgctgc tgccttggac
     1741 ggttcggggt tttagtaatg acaatgaatt tgtaaaattc aacaaagaat caaacaaata
     1801 cagccaaaga taccgcatcc gcgaaaacgg caacaacggc aagcgcaatt tgggcgacat
     1861 cgtcaacagc cccatcgtgg cggtcggcgg gtatttggct acttccgcca acgacgggat
     1921 ggtgcatatc ttcaaaaaag gcaacgggga cg
//
LOCUS       DAWFJR010000163         1846 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWFJR010000163 DAWFJR010000000
VERSION     DAWFJR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.163"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(16..222)
                     /locus_tag="WGR04_002054"
     CDS             complement(16..222)
                     /locus_tag="WGR04_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795531.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRH"
     gene            complement(219..764)
                     /locus_tag="WGR04_002055"
     CDS             complement(219..764)
                     /locus_tag="WGR04_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0795532.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGRHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAARV"
     gene            complement(920..1348)
                     /locus_tag="WGR04_002056"
     CDS             complement(920..1348)
                     /locus_tag="WGR04_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ0795533.1"
                     /translation="MAFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(1329..1763)
                     /locus_tag="WGR04_002057"
     CDS             complement(1329..1763)
                     /locus_tag="WGR04_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ0795534.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
BASE COUNT          443 a          539 c          377 g          487 t
ORIGIN      
        1 caacctgtcc catgattaat gacgattctt gcgtttgcgc gccgcacgtt tcgcggctgc
       61 cacgcctgag tttcttaaat ttgggcgtgg ataggtgtgt atcggacgaa catttggcat
      121 ctgtataagt gcgcggttta cctgctcatt actaggattt tctccaatca attttgtaag
      181 aaaataggaa gcagcttctt tcaatttacc aataatattc atactcttgc cgcccccaat
      241 tccatcgcaa tcgcgtccgc aatcgcgcgg cagatgcccc atttggtcgt cttaaacaag
      301 gccaaatcag tgtcgttgct gataaaaaac ggctcaaaca caatgccgcc ggcttgcgca
      361 taagccaggc gcgaatgccg acccgcattg tcgggtttaa agccgtcttc gccgcgcagt
      421 ttccagccgg ttttcttggc aacggctttg ctcaacacct gacaccagcg tttgttttta
      481 acggtactca aggcttcgat gcctgtcgcc gctttgctga cggcagcgtt ggtgtgaaac
      541 tcaatcgcca catccgagcc gcgaatcagt tttacggctt cacgcagcgg catattgcct
      601 ttgcctgtgc cgtcggtttt aacagtcaaa ccgtagtcat cgcgcaaaat agcagccacg
      661 atattgcgca tatcctgcgc caagtccgcc tcacggtcgc tgccgttgac cgcacccggg
      721 tcggtgttgc tgtgtccagc ggttaaggtt acggttttgc ccattcatca tctccaaaaa
      781 aggttgattg cataacattt gaaaacccca ttaaaccgtc tttaacccat cgtcctgaac
      841 ggtagatgtt tcaccccctg cgaaccaaaa aaaagaccgt ctgaatacag acggccaaag
      901 cctggtcaca catcactgcc taaaataaag ctgcctgctg tgccgcaggt cggctcattt
      961 cattgataat cgtatatccc gttcgtgaag agatgccgta tttagggcat agcttcgtca
     1021 tcgccataag cccgctctcc ttatcaatat cgcgcaattt gacaaactcc tgataaaacc
     1081 tatggtttct caactgtatt aacgccttac cgcaccgtgg gacatacaat tcctcgccac
     1141 catatacctg caacagctca tgtgttttca cttcgccgat ggcttcgacc aaaattgcca
     1201 aacgctcggt gtccaccttg cccttaccaa atttaaaccg cgccccgcca atcgccttga
     1261 ccagctgttc cgtcgctgcc agtccgatga catccacaat gtccaacacg gtatccggca
     1321 ataaatgttc aactttttcg aacgccatca tccccacccg ctttttccgt tttcctgttt
     1381 tccgcaatct gcaacgcagc aaccagttta tgtagctgcg tatcgtctaa atattcgacc
     1441 ttatccttac caaacatccg ccgcgccatt gcgtgtgcat agttccaatg tttgccgccg
     1501 acggtcagca gggcttcgac tttgtccaac attgccgctg atgatgtccg acgcagatgt
     1561 ggtttaccgt gtgggttacc ttttgcttta ggcttaaatc cgtgcgaccg catatcagcg
     1621 acaacagact caagttcgga aacatccata tccgcacacg accgcttgcc cgtcacgcgc
     1681 tccaacaccg cgcgataggt accgtcgtcc aagcccagct ccttttgagc aatcttaatt
     1741 ttcgcaatca acgcccggcg catctcaaac ccataaaaca caatatattg actaattaac
     1801 gcatattata ccaataaaat acaatatata gtattaagcc gatgtt
//
LOCUS       DAWFJR010000164         1826 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWFJR010000164 DAWFJR010000000
VERSION     DAWFJR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.164"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(461..1456)
                     /gene="porB"
                     /locus_tag="WGR04_002058"
     CDS             complement(461..1456)
                     /gene="porB"
                     /locus_tag="WGR04_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ0795535.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVAHNGAQA
                     ASVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHHQVQENVNIEKYQIHRLVS
                     GYDNDALYASVAVQQQDAKLVEENYSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSF
                     DATNYNNDYDQVVVGAEYDFSKRTSALVSAGWLQEGKGESKFVSTAGGVGLRHKF"
BASE COUNT          484 a          399 c          465 g          478 t
ORIGIN      
        1 cgtcctgaat tcccactttc gtgggaatga cgggatgtag gttcgtagga atgacgtggt
       61 gcaggtttcc gtacggatgg attcgtcatt cccgcgcagg cgggaatcta gacattcaat
      121 gctaaggcaa tttatcggga atgactgaaa ctcaaaaaac tggattccca ctttcgtggg
      181 aatgacggga ttaaagtttc aaaatttatt ctaaatagct gaaactcaac gcactagatt
      241 cccgcctgcg cgggaatgac ggcatatttt gacattgaat aaaaaagact aaaaacagga
      301 aaaagccaaa cagaaaacag cgaaacagaa aaaagccaaa cagaaaaaag ccaaacagaa
      361 aacagaaaac agccaaacag gaaaaagcca aacaggaaaa agccaaacag aaaaaagcct
      421 gtctggcgac aggctttttg ttgataccaa tctttgcaga ttagaatttg tggcgcagac
      481 cgacaccgcc ggcagtcgat acgaatttgc tttcgccttt gccttcttgc aaccaaccgg
      541 cagaaaccaa ggcagaagtg cgtttggaga agtcgtattc cgcaccgaca accacttggt
      601 cgtaatcgtt gttgtagttt gtagcatcaa acgagccttt gaagccgtgg gcgtaagaaa
      661 cgcggggcgt tacgttgccg aagcggtatg ccaaggtagc ggcaacttcg gtttgagagt
      721 tgtgcgaata attttcttca accaatttgg catcttgttg ttgtacggct acggaggcgt
      781 acagggcatc attgtcgtaa ccgctgacca aacggtgaat ctggtatttc tcaatattca
      841 cgttctcttg cacttgatga tgtcttttat aggcaccgcc atattgcacg aagaagccgc
      901 cgtttttgta gttgaagccg gcgtggtaag attcgctgtt atgtctgcct gcattgtcgt
      961 taagcgcgta ttgtacgctg ccgctgaggc cggcaaattc gggagaatcg tagcgtacgg
     1021 aaatgaggcg tgcttcgggt tcggcaattt tgtttacacc caaatagtcg cttttgctat
     1081 cccaaggatt gatgtcgccg gtatctttca ggacgctgtt caaacggccg acgcgcaatt
     1141 taccgaagcc gcctttcaag ccgatgaagg attggcggtt gccccaaccg gagtcagtac
     1201 cggcgataga tgctttttgc tcaacctgcc aaatggcttt caggccgtta ccgaggtctt
     1261 cttggccttt gaagccgatt ttcgaaccca aatcaacgat gccggtaccg gtttcaacgc
     1321 tagccgcctg agctccattg tgagctacgg agcgggaagt ttctacgccg gctttgatgg
     1381 tgccgtacag ggtaacgtca gccattgctg caacaggaag ggctgccaaa gtcagggcaa
     1441 tcagggattt tttcattgct gtattccttt tttggttaag aaatttaagc aggccgggct
     1501 ttccaagccg cttagctttg catttaccgc cgacgttttc ggcgattcca tgtctgaaac
     1561 tatagatgtt cctgtctgaa aaaacaaact tctgcctgtt tttgtgtgga attcgatgtg
     1621 tattttgaag ggcggataga gatattgtgg gtattttttg ttttataaca tatggttatt
     1681 taaatttttt aagatttgca tttttacaac acttactcgg gagggtattg gagggcattg
     1741 caaaccgagg gttataaaga cggcaaaaaa cgcgcacgtg gtttctttcg gaacgggcgg
     1801 atgcaaaacc cgcccctgcg gcagga
//
LOCUS       DAWFJR010000165         1772 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWFJR010000165 DAWFJR010000000
VERSION     DAWFJR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.165"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(300..1454)
                     /locus_tag="WGR04_002059"
     CDS             complement(300..1454)
                     /locus_tag="WGR04_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ0795536.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGNNIQLQLTVPP
                     SQGQTGNKVTKGKSRIRTKISDFGSFIGFKGGEDLGEGLKAVWQLEQDVSVAGGGATQ
                     WGNRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDDMSVS
                     VRYDSPEFSGFSGSVQFVPAQNSKSAYKPAYVDDQGKVKGPVVGKPGSDVYYAGLNYK
                     NGGFAGNYAFKYAKHANVGRDAFNLFLLGRIGDDDEAKGTDPLKNHQVHRLTGGYEEG
                     GLNLALAAQLDLSENGDKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERGKKGEN
                     TSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          455 a          448 c          401 g          468 t
ORIGIN      
        1 cctacttttt gctcaatatt aggaaggttt taggcaattg aaaatttttt ggcgcatttt
       61 tatgcgtcaa atttcgttaa cagactattt ttgcaaaggt ctcactatat gtgcaaacca
      121 agccaaaaat gcgaaatacc gtctgaaaat ctttcagacg gtatttgctg tctttattgc
      181 cgtttttctt ccgtatccgg atttttgttt ggggctgaag cagattggca gtcagattgc
      241 aatcaaagaa tgaaggcgag ccgtcaaaaa caaagctatc cgcttcaccg ccccgatatt
      301 tagaatttgt ggcgcaaacc gacggaggcg gcattaattt gagtgtagtt gccgatgccg
      361 gtattgcgtt tcagccaagc gccagacacg atggcggaag tgcgtttgga aaaatcataa
      421 tcaacgccgg cgatgatttg atcatagctg gtattttcgc cttttttacc gcgttcgata
      481 aagtcgaaac catgggcata gctgatgcgc gggactgcat taccgaagcg gtaggaagca
      541 gtcgcggcaa tttcggtcgt actgtttttg gttttgtcgc cattttcaga caaatccaac
      601 tgagccgcca aggcgagatt caagccgcct tcctcatagc cgcccgtcag gcggtgtacc
      661 tgatggtttt tcaagggatc ggtacctttg gcttcatcat catcgccgat gcgcccaagc
      721 aagaacaaat taaaagcatc acggcccaca ttggcgtgtt tcgcatattt aaaggcatag
      781 ttcccggcaa aaccgccatt tttgtaattc agaccggcat aatacacatc cgatccgggc
      841 ttgccgacaa caggcccctt aacttttccc tggtcatcca cataagccgg cttataggcg
      901 gacttgctgt tttgggccgg aacgaattgg acgctgccgc tgaaaccgga aaattccgga
      961 gagtcgtaac gtacggaaac cgacatatcg tcgtggcgtt tgaaaatacc caattgcgaa
     1021 gccacatcat tattgctgtc ccaaggatca atggcttggc tggcatcgtc aaactgattc
     1081 gcaacacggc cggcgcgcag cgtgccgaat tcgcctgcca agccgataaa ggattccctg
     1141 ttgccccact gggtcgcgcc gccgccggca acggatacgt cttgctcaag ctgccaaaca
     1201 gccttcagcc cttcgcccaa atcctcgccc cccttaaagc cgataaacga gccgaaatcg
     1261 ctgattttcg tcctgatgcg gcttttgccc ttagtaactt tattgcccgt ctgaccttgt
     1321 gagggtggta cggtcaattg cagctgaatg ttgttgcctt ccacgccggc tttgatttcg
     1381 ccgtataggc tgacatcggc aacggccgca agcggcagtg cggacaatac gagggcggta
     1441 agtttttttc gcatatcggc ttccttttgt aaatttgata aaaacctaaa aacatcgggc
     1501 aaacacccga tatgtcttca attatacccc cccccccgca aaaaaccatt tttcagaaca
     1561 aatatctgat aaatgccgca acctttattt taaaaatgat tatattttga tataaaacaa
     1621 tagcttattt tttcaaaaac cgttgtgttt ctacaacaca attcaagcgc agacctcgtg
     1681 cgggccgatg cgctgctgcc cggatgcagt ctcggctttt taaaacgcca taaaaaaaca
     1741 cacgcggcac tttatagtgg attaacaaaa ac
//
LOCUS       DAWFJR010000166         1737 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWFJR010000166 DAWFJR010000000
VERSION     DAWFJR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.166"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(28..351)
                     /locus_tag="WGR04_002060"
     CDS             complement(28..351)
                     /locus_tag="WGR04_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ0795537.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(339..578)
                     /locus_tag="WGR04_002061"
     CDS             complement(339..578)
                     /locus_tag="WGR04_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ0795538.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(701..1498)
                     /gene="truA"
                     /locus_tag="WGR04_002062"
     CDS             complement(701..1498)
                     /gene="truA"
                     /locus_tag="WGR04_002062"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ0795539.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALENALAQI
                     AGEAVSTIVAGRTDTGVHATAQVVHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESVPVRSPLLKNRAGWTHLKLDIEPMRRAAAYLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIVRPQIPEWL"
BASE COUNT          388 a          411 c          504 g          434 t
ORIGIN      
        1 tgaacacaat ccattcagac tattcaatca ggcaaacatc tcctgcaata ctgcaaacag
       61 tttttcagcc gtactgttgt ctaaattgcc aagatgcttg accaatccgg ctttatccac
      121 agccctaatc tgttcgggca aaagcaaacc gtctttatcc tgaaagcgga cattgacgcg
      181 gaacggggca ggacggcttc cgctcgtcat gggaacgatc agcacagtct tgagatagtt
      241 gtgtatttca ggaggagaga ctacgacaca aggacgtgtc tttttgattt cgcttcctac
      301 ggtcgggtct aaggagacca gatagattcc gccgcgtact accatatcca ttctttatcc
      361 gcttcgtttt caatttcgga aaaaaaatgc tcctgctcgg tttcgacaag cattgcggca
      421 gcttctgccc atcccctgcg aacggtagga cagcttaaaa tgatattgcc cttttcaact
      481 gtaacagcca agctgtccac tgcccctatt tgacccaata atgatttggg cagaatcacg
      541 ccttgcgagt ttcccatttt gcgtatgttg agaatcatat acgtaccata ttcacctgtt
      601 tatgtaataa caatgttatt accttgatga ggtagtgtca acattgaaaa agattgcctg
      661 acagtttgtc cgatttcaaa atctccgcga caagaatgtt ttaaagccat tcggggattt
      721 gggggcggac gatgccatat gcctcgggat agtcgacgcc ggtcaggtag agtccgtcgg
      781 gcatgaaggt cggtggggct ttgaggcggc tgcgttcttg aatcagtgcg gcgaagcctt
      841 cgacgctgag tctgccgctg ccgacataaa cgagcgcgcc catgatgttg cgtaccatat
      901 ggtgcaaaaa ggcgttgccg tgcaaatcga ggcggacgag tcctgagctt tgggtaaggt
      961 cggcgcggta gatggttttg acgggggatt ttgcttggca ttcggcggcg cggaagctgg
     1021 agaagtcttg ttcgccgatc aaataggcgg cagcccgacg catcggttcg atgtcgagtt
     1081 tgaggtgtgt ccagcctgcc ctgtttttga gcagggggga acggacgggg acggattcga
     1141 gcaggtagcg gtaatgccgt ccgtatgcgt cgaagcgggc gtgaaattcg ggggcgacct
     1201 gtcgggcgtg caaaacggca atgccttcgg gcaggtgggc atttacgccg cgcacccatg
     1261 cctgttgggg acgggcggca gttgtgtcga agtggacgac ttgggcggtg gcatgcacgc
     1321 cggtgtcggt cctgccggca acaatggtgg aaaccgcttc ccctgctatt tgggcaagtg
     1381 cgttttccaa tgccgcctga acggtcggta cgccgtcagc ctgtttctgc cagccgtaaa
     1441 agcggctgcc gtcataggat agggttattg cccagcgttg tttttgtgcg gtatccatcg
     1501 gatttgggat tcggataaat gttcagactg cattgtatcg cagattttgc agggaaacgg
     1561 caaacgcccg gggcgagcgg cgttgtttgg ggagttgggg ggggtggatg cagttgctac
     1621 gaatcgctat cctgtgaatt caccctgtca ggagtgcccg aatcgtcatt cccgcgcagg
     1681 cgggaatcta ggacgtaaaa tctaaagaaa ccattttata gtggattaac aaaaacc
//
LOCUS       DAWFJR010000167         1707 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWFJR010000167 DAWFJR010000000
VERSION     DAWFJR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.167"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            58..1431
                     /locus_tag="WGR04_002063"
     CDS             58..1431
                     /locus_tag="WGR04_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0795540.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQSY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPKQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKTEMREPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
     gene            1611..>1707
                     /locus_tag="WGR04_002064"
     CDS             1611..>1707
                     /locus_tag="WGR04_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEZ0795541.1"
                     /translation="MSTQFDVAVIGAGPAGSVASALLRKKGYSGLN"
BASE COUNT          441 a          555 c          410 g          301 t
ORIGIN      
        1 ccatcctatc ggtttcccgc ttcagacggc ataaggtctg aaagaaagac tacaattatg
       61 agtaatccat tttcctcttt aggtttgggt acggaactcg tttccgcact gaccgcgcaa
      121 ggttacgaaa acccgacgcc catccaagcc gccgccattc ccaaagcact cgccggtcat
      181 gatttactag ccgccgcgca aaccggcacg ggcaaaaccg ccgcctttat gctgccctcg
      241 ctcgaacgcc tcaaacgtta cgccaccgcc agcacctcgc ccgcgatgca ccccgtgcgt
      301 atgctcgtgt tgacccccac gcgcgaactc gccgaccaaa tcgaccaaaa cgtgcagtcc
      361 tacatcaaaa acctgccgct gcgccacacc gtcttgttcg gcggtatgaa tatggacaaa
      421 cagaccgccg atttgcgtgc cggctgcgaa atcgtcgtcg ccacagtcgg acggctgctc
      481 gaccacgtga aacaaaaaaa catcagtttg aacaaagtcg aaatcgtcgt tctggacgaa
      541 gcagaccgca tgttggatat ggggtttatc gacgacatcc gcaaaatcat gcagatgctg
      601 cccaaacaac gccaaaccct gctcttttcc gctaccttct ccgcaccgat acgcaaactg
      661 gcgcaagact tcatgaacgc gcccgaaacc gtcgaagtcg ccgcgcaaaa caccaccaac
      721 gccaacgtcg aacaacacat catcgccgtc gataccattc agaagcgcaa cctgctcgaa
      781 cggctgattg tcgatttgca tatgaaccag gtcatcgtgt tctgcaaaac caaacaaagc
      841 gtcgaccgcg taacgcgcga actggtgcgc cgcaacctgt ccgcacaggc gatacacggc
      901 gaccgttccc aacaaagccg gctcgaaaca ctcaacgcct tcaaagacgg cagcctgcgc
      961 gtcctcgtcg ccaccgacat cgccgcgcgc gggctggaca ttgccgaact gcccttcgtc
     1021 atcaattacg aaatgcccgc ccagcccgaa gactacgtcc accgcatcgg gcgcacgggg
     1081 cgcgcgggcg cggacggcgt ggcgatttcc ctgatggacg aatccgaaca gaaaatgttt
     1141 gaatccatta aagagctgac cggcaacaag ctgctcatcg agcgcatcga gggcttcgag
     1201 ccgcaatggt gggaacaggg cggcgcaaaa ccggaaaaaa ccgaaatgcg cgaaccgaga
     1261 caacgcaacc gctacgaatc cgccagggct cagcgcgaaa aaaatacccg accggaaacc
     1321 acggcaaacg atgcgggcgc ggcttgcgga aaaattgccg gacgcagccg ccgaagccgt
     1381 cgggaacacc ggacgtgcgc cctgctccaa ccgcgttacg gcgtaaaata accctgaaaa
     1441 tcaaatgccg tctgaacatt tcccgtttca gacggcattt ttcaaaccgg actgacgcat
     1501 cgggagcaac cgcccgcacc ggataaattt cttccgcaaa cagtttcaga cggcatttgc
     1561 tgcctgtaca atatcatttc cttttccact caaaccatca ggatttccca atgtccaccc
     1621 aattcgatgt cgccgtaatc ggcgcaggtc cggcaggctc ggtcgcatcc gccctgctcc
     1681 gcaaaaaagg ttatagtgga ttaaatt
//
LOCUS       DAWFJR010000168         1635 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWFJR010000168 DAWFJR010000000
VERSION     DAWFJR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.168"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(387..1040)
                     /locus_tag="WGR04_002065"
     CDS             complement(387..1040)
                     /locus_tag="WGR04_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0795542.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLVLAADEVFEGPVEQDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTDSLSSYDKLDVSGFIHYRINHSKECADRQ
                     NHINGIENFWNQAKRVLRKYNGIDRKSFPLFLKECEFRFNFGTPSQQLKILWDLGLI"
BASE COUNT          488 a          361 c          336 g          450 t
ORIGIN      
        1 cttttccgat aaatgaccgc aacctaaaac ccatccttcc cgcaaaaaca gaaaaacaaa
       61 aacctaaaat cccgtcatcc ccacgataac agttgcgtaa ttgcgtagag tgggcttcag
      121 cccaacgttt tttctttttc ggtcgttgat tggtgggctg aagcccaccc ttgtatatcg
      181 gaactcccgt atcatagcaa caaaccgccc ggccgccacc cgcgcccacc caaggcacac
      241 aaccgttgcg cagcacaggg agcggcaggg caacccatcg acacaaccgg acagttgccg
      301 gacaacacaa ccgaatgcaa ggcaggttga tgatgagtac ccgataccat tacgcaggta
      361 tagtgaatta aatctaaggg gctgtactag attagcccta aatcccacag gattttaagc
      421 tgttgagacg gtgtgccgaa gttaaatcga aattcgcatt ctttcaagaa cagcgggaaa
      481 gatttgcgat cgattccatt gtattttcgc aagacgcgtt ttgcctgatt ccaaaagttc
      541 tcaatgccgt taatgtggtt ctgacggtct gcacactcct tggaatggtt gatgcggtaa
      601 tggataaaac cgctcacgtc caacttgtcg tagctgctca gactatcggt ataaacaata
      661 ctatccggca tgattttctt tttgatgaca gggagtaacg tttcagactt ggcattatcc
      721 accacaacgg tatagacccg tccgttgcgt ttcagaatgc cgaagacaac cacttttcct
      781 gccgcaccgc gaccacgtct gcctttacgc cgtccgccga aatcgctttc gtcctgctcg
      841 acagggccct caaaaacctc atcggcagcc aagaccaaat gatggttgat aaccgtgcgg
      901 attttacggt agaacagtgc tgccgaattg ggatggatac ccaaaatatc ggcggcagaa
      961 cgggcggtaa cttcgagtac aaaaaaacgg agcagttctt tctgtacttt tttctttaat
     1021 ttgcagtgcg ttatcttcat atttcgaggg taacatatct gctaatctag tacagcccca
     1081 aaaatatacc aaaaacagca aaacaaattg taaggatacg tataggcttt gtaaaggtaa
     1141 attgtgaaaa aagcagtttt ttaaacgaat gaaacggctt cgggctgaaa tatatgctga
     1201 tgccctgtcc ttcccgtata tcttgtgtgt tgtcaaagtg caggctgctt tgaaattggc
     1261 attacagtcg atgaaccacc attttgcttt atttcagcgg gcttgagatg tatataagaa
     1321 tattgttttg aataaattta aagaaaatga taatcgttat tgacgatttt taaaggaaag
     1381 cgtagagtgc caattctatg aagcaatacg gtaagtaaca atgaaaatat ctactgcttg
     1441 ggtatagagc atatttcaca acccgtaact attcttgcgg aaacagagaa aaaagtttct
     1501 cttctatctt ggataaatat atttaccctc agtttagtta agtattggaa tttataccta
     1561 agtagtaaaa gttagtaaat tatttttaac taaagagtta gtatctacca taatatattc
     1621 tttaactaat ttcta
//
LOCUS       DAWFJR010000169         1514 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWFJR010000169 DAWFJR010000000
VERSION     DAWFJR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.169"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..>1514
                     /locus_tag="WGR04_002066"
     CDS             <1..>1514
                     /locus_tag="WGR04_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ0795543.1"
                     /translation="IIMNEQNQPEVKQEGSYSTLREKDRERKFIYNKGGSGGGSVSFN
                     NSDELVSQQSGTAVFGTATYLPPYGKVSGFDADALKERNNALNWINTTRPGLAGYSYA
                     NVICRDTRQCPQLFYETKFAFSNKDLVNNAGRLDRHPDPSRENSPIYKLKDHPWLGVS
                     FNLGSEGTAKDGKVSNKLISSFSEDNNNQTIVSTTRDHPISLGDQQREHTAMAYYLNA
                     KLHLLDKKGIKDIAQGKTVDLGILRPRVETKTNNWGNLLNFWVTWKIEDNGQIPVKLG
                     LPEVKAGRCTNAAHPNSKAKPPSPALTAPALWFGPVQNGKVQMYSASVSTYPGSSSSR
                     IFLQELKTQTDPGRPGRYSLAALDAQNIKSHEPRFNGRQTVIRLPGGVYKISLGRNGG
                     RVAGINGNDGKNDTFGIFKDRYVTPEADEWSEMLLPWTARYYGNDTPFETNRQKNQKK
                     YSQKYRIRTKEDDNDKPRDLGDIVNSPITAVGGYLATSANDGMVHIFKKGNGVD"
BASE COUNT          431 a          408 c          380 g          295 t
ORIGIN      
        1 tattatcatg aacgaacaaa atcagccgga ggtaaagcag gagggttcat attcaacctt
       61 aagggaaaaa gacagggaac gcaaatttat ctataataaa ggcggatcgg gtggaggctc
      121 tgtctcattc aacaatagcg atgagcttgt ttctcaacaa agcggtactg ccgtttttgg
      181 cacagccacc tacctgccgc cctacggcaa ggtttccggt tttgatgccg acgctctgaa
      241 agagcgcaac aatgccctta attggattaa tacgacccgc ccggggttgg caggctactc
      301 ctacgccaac gtcatatgca gagacacaag gcaatgcccc caacttttct atgagaccaa
      361 atttgccttc agcaacaaag atttggtaaa caatgcgggc aggctggata ggcacccgga
      421 cccaagccgc gaaaattcgc ccatttacaa attgaaggat catccatggt tgggcgtatc
      481 tttcaatttg ggcagcgagg gtaccgccaa agatggcaag gtatccaaca aattgatatc
      541 ttcttttagt gaagacaata ataatcaaac catcgtctct acgacacgag accaccctat
      601 ttcccttggc gaccagcagc gcgaacatac cgccatggcc tattatctga acgccaaact
      661 gcacctgctg gacaaaaaag ggattaaaga tatcgcccaa ggcaaaacag tggatttggg
      721 catcttgaga ccgcgcgtcg agacgaagac aaataattgg gggaatctgc taaatttttg
      781 ggttacgtgg aagattgaag ataacgggca gattccggtc aagctcggcc tgccggaagt
      841 caaagccggg cgctgcacca acgccgccca ccccaattcc aaagccaaac ccccttcgcc
      901 ggcactgacc gcccccgcgc tgtggttcgg ccctgtgcaa aatggtaagg tgcagatgta
      961 ttccgcttcg gtttctacct accccggcag ttcgagcagc cgcatcttcc tccaagagct
     1021 gaaaactcaa accgaccccg gcaggcccgg ccggtattcc ctcgccgctt tggatgcgca
     1081 gaacatcaaa agccacgagc cgcgtttcaa cggaaggcag accgtcatcc gattgccggg
     1141 cggcgtgtac aagatcagcc tgggcaggaa cggcggccgg gtcgcgggta ttaatggcaa
     1201 tgacggcaaa aacgacactt tcggcatctt caaggacagg tatgtcacac ctgaggccga
     1261 cgagtggagc gaaatgctgc tgccttggac ggctcggtat tacggtaatg acactccatt
     1321 tgaaaccaac agacaaaaaa accaaaaaaa atacagccaa aaataccgca tccgcacaaa
     1381 agaagatgac aatgacaaac cccgcgattt gggcgacatc gtcaacagcc cgataacggc
     1441 ggtcggcggg tatctggcaa cttccgccaa cgacgggatg gtgcatatct tcaaaaaagg
     1501 caacggcgtg gacg
//
LOCUS       DAWFJR010000170         1497 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWFJR010000170 DAWFJR010000000
VERSION     DAWFJR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.170"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..439)
                     /locus_tag="WGR04_002067"
     CDS             complement(<1..439)
                     /locus_tag="WGR04_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0795544.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNRLAQDDTLSELLELINCQLTEKGLKVEKASAAVVDAT"
     gene            724..1395
                     /locus_tag="WGR04_002068"
     CDS             724..1395
                     /locus_tag="WGR04_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0795545.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVNISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
BASE COUNT          394 a          330 c          383 g          390 t
ORIGIN      
        1 tggtggcgtc aacgacggcg gcggatgctt tctctacttt taagcctttt tcggtcagtt
       61 ggcagttaat cagttccaac agttcggaca gggtgtcgtc ttgcgccagc cggttgcggt
      121 aacggcataa ggtgctgtaa tcggggatgc tcagttcgtc aaaacggcaa aacaggttga
      181 aatcgatgcg ggtgatgagg ctgtgttcga gttcgggatc ggagaggctg tgccattgtc
      241 cgagcaggac ggctttgaac atggacaaca ggggataggc gggacgaccg cggtggtctc
      301 ggaggtaacg ggttttttga cggttcaggt actgctcgat cggctgccaa tcaatcacct
      361 ggtccaactt caatagcggg aaacggtcga tgtgtttggc aatcatggct tgggcggttt
      421 gctggaagaa ggtgctcatg agaaatcccc taaatgtctt ggtgggaatt taggggattt
      481 tggggagttt tgcaaaggtc tcaaacggat gaaatcaacc gatattcaaa cacagtcatt
      541 tttagcgcat tttcagcgta tcgttaatgc ggaaaatttc gtgaacaggt tttttgcaca
      601 ggtctccgcc tcgtttcgcg ggatgggaaa ccgtattaga aaacggacgc acgcaacata
      661 aaccccgaaa gtgatgataa gatgatgatt taacgtactg ctttaattat ttaaggaatt
      721 atcgtgtttc ccgacaaata caagttgagt ttggaagaaa atatttttct ggcaaagaaa
      781 gtattggttg cccaaattca caacctcagc cgttttgaga attgtcagac gaccttgttg
      841 cagaccgaac aaattatcca tggcaaaaat gtagcctccg cgtcactgga agacatccaa
      901 accatcttga acctgaaacg tgcctatcaa tatgtgattt cgcatatttc aaatggcgaa
      961 ccggtcaata tttcactcct taaaaaaatc aacaacattg ttgccaagga cgattctttg
     1021 gcacccggtg atttccgtac cggttcggtc ggcgtaacgc tattggacgg ttcccgtcat
     1081 gccccgaatc cagtgaagga aattgaagtg gcccgcgtgt tacaaaatat cggactgcaa
     1141 agcggttcga cgacggaggc agccgtccgt tttatgcttt attgtatgcg gcagcaggtt
     1201 ttttgggacg gcaacaaacg aacggcaacc ttatttgcca acggtctgat gatggcgggg
     1261 ggctgcggca tcttggaaat ctccgaaatg cagatgccgc aattcaatga aaaactgtcc
     1321 gcattctatc gctccggcga cgataccgat atttccaagt ttgtgtatca aaattgtata
     1381 tcgggcatag actgagacct ttgcaaaatc ccccaaaatc ccctaaattc ccaccaagac
     1441 atttaggaga tttctcatga gcaccttctt ccaacaaacc gcgcaagcca tgattgc
//
LOCUS       DAWFJR010000171         1460 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWFJR010000171 DAWFJR010000000
VERSION     DAWFJR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.171"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            232..1416
                     /gene="ubiM"
                     /locus_tag="WGR04_002069"
     CDS             232..1416
                     /gene="ubiM"
                     /locus_tag="WGR04_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ0795546.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKARLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
BASE COUNT          392 a          423 c          316 g          329 t
ORIGIN      
        1 aaggcgaggc aacgccgtac tggtttaaat ttaatccact atacataccc cacacccgat
       61 ttgatacaca ttaagctgaa agtaaaaatc cgcatacacc ctcccctgca tatttcttca
      121 acagtgggtt ttgatataat cgcctatctg ttacagatag ttcaaacggc attcaaaccc
      181 ttacaaatac cgtctgaaat catcggcatc cgtctatgca aaggatattt tatgagttta
      241 cacagcgaca tcctcgtcgt cggcgcaggt ccggcgggtt taagttttgc agccgaactt
      301 gccggcagcg gtttgaaagt taccctgatc gaacgcagcc cactgactgt tctgcaaaat
      361 cctccatatg acggacgcga aatcgccctg acccattttt cccgcgaaat catgcagcgt
      421 ctgggtatgt gggacaaaat tcccgaaaat gaaatttacc ctttgcgcga tgccaaagtg
      481 ctgaacggac gttccgacta ccagctccac tttccccaac cgaccgaggc gcgcggcgaa
      541 cctgccgact gtttgggcta tcttatttcc aaccacaata tccgacgcgc tgcctacgaa
      601 gtcgtatccc aactcgacaa cattaccatt ctgaccgata cggttgtcaa agaagtcaaa
      661 acatctgaca acgaagcaca agtcattttg gaaaacggca aaatcctgac tgcacgcctg
      721 cttttggcgg cagacagccg cttctcgcaa acccgtcgac aactcggtat ctcctccgat
      781 atgcacgatt acagccgaac catgttcgtc tgccgtatga aacataccct ttccaaccaa
      841 cataccgctt acgaatgctt ccattacgga cgcaccatcg cactgctgcc gctggaagag
      901 cacctgacca ataccgtcat taccgtcgat accgataaaa tcaacagcgt tcaaaacctt
      961 tcgcccgaag aattggcggc aagcgtaaaa gaacagctca aggccaggct cggcgatatg
     1021 gaactggtca gcagcatcca ccattacccc cttgtcggta tgattgccaa acgtttctac
     1081 ggcaaacgca gcgcgctgat cggcgatgcg gctgtcggta tgcaccccgt taccgcacac
     1141 ggtttcaact tgggtctgtc cagcgcagat attttggcaa aactgatact cgaagccgag
     1201 cagcgcggtc aggacatcgg cgcggcttcc ttgttggaaa aatacagcag caaacatatg
     1261 ctgcacgccc atccgcttta tcacgggacc aatatgatgc tgaagctgtt taccaacgaa
     1321 accgcaccgg caaaactgct gcgcggcttg gtattgcgtg ccggcaacaa tttcccgccg
     1381 ctgaaaaagc tgattaccaa acaactgacc ggttgaaaac tgccgaaatg ccgtctgaaa
     1441 cgtttcagac ggcatttttg
//
LOCUS       DAWFJR010000172         1445 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWFJR010000172 DAWFJR010000000
VERSION     DAWFJR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.172"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            27..1364
                     /locus_tag="WGR04_002070"
     CDS             27..1364
                     /locus_tag="WGR04_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0795547.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGKNAVDSFRALRPSTQWLWVGRMGVSACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIAATIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYLISAVIMPVGALSVA
                     VFTAWIQDRQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
BASE COUNT          264 a          340 c          389 g          452 t
ORIGIN      
        1 tatcttcaca tttcttaata aattttatga gtaaccatac ttcttggtcg tccaaaatcg
       61 gtttcgtgct tgctgcggca ggttcggcca tcggtttggg cgcgatttgg aaatttcctt
      121 atacggcagg caccaacggc ggcgcggtgt ttttcctgct gtttttgata tttaccgtct
      181 tggtcgccct gcccgtccag cttgccgagt tttatatcgg gcgcacaggc ggtaaaaatg
      241 ccgtcgattc cttcagggct ctgcgtccga gtacgcaatg gctttgggtc gggcgcatgg
      301 gcgtttccgc ctgctttatt ttgctgtcgt tttacagcgt ggtcggcgga tgggtattaa
      361 attatgtcgt ccacagtttc acgggggcgg ttcataccgg cgcggacttt gaagccttgt
      421 tcggcgcgac gatttccaat ccggcaggtt cgctgtccta tcaggcactg tttatgctga
      481 ttacggtttg ggtggtcaaa ggcggcattt cagacggcat tgaaaaggca aaccgttatc
      541 tgatgccggg gctgtttatc ctctttattg cgctggcaat ccgttcgctg acgctgccgg
      601 gtgcaatgga gggcgtgtct ttcctgctca aaccgaattg gtcgtacttt aaagccgata
      661 cgatgattac ggctttaggt caggcgtttt ttgccttgag catcggcgtt tccgctatga
      721 ttacctatgc ttcgtatttg ggaaaagatc aggatatgtt ccgctcgggg catacgatta
      781 tgtggatgaa cctcttggtt tcgctgttgg caggtttggt gatttttccg gcggtgttcg
      841 ccttcggttt tgaaccgagc caggggccgg gattgatttt tatcgtattg ccggcggtgt
      901 ttatgaaaat gccgttcggt acggttttgt ttgcggtatt tatgctgctg gtcgttttcg
      961 ccacgctgac ttcggcattt tcgatgttgg aaacggtcat cgccgcaacc atccgccaag
     1021 acgagcgcaa acgcaaaaaa catacttggc ttatcggcac ggctattttt attgtcggca
     1081 tcccgtccgc gctgtctttc ggcgtatggg gtgagtttaa ggttttcggc aaaaccattt
     1141 ttgatttgtg ggactacctt atttctgcag tcatcatgcc tgtcggcgca ttgagcgttg
     1201 ccgtatttac cgcttggatt caggacaggc agtctgtgtt aaaagatgcc ggcgcgggca
     1261 gcaccgtacc acgggcagtg ttgctgctgt ggctgaatac cttgcgctac ctcgccccga
     1321 ttgccattat tattgttttc atcaattctt tggacatcct ttaaaagcca tccaaacagc
     1381 aaaaatgccg tctgaaagcc tttcagacgg catttttgct tcaggttcag cctatttcgt
     1441 tcaaa
//
LOCUS       DAWFJR010000173         1412 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWFJR010000173 DAWFJR010000000
VERSION     DAWFJR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.173"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..>1412
                     /locus_tag="WGR04_002071"
     CDS             <1..>1412
                     /locus_tag="WGR04_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ0795548.1"
                     /translation="SYIPGTMPRKDIENNDSTLAKELRAFAEKGYVGDRYGVDGGFVL
                     RQVELSGQKHVFMFGAMGFGGRGAYALDLTKADGNDPTKASLFDVKDNGNNGNNGNNS
                     VQLGYTVGTPQIGKTHNGKYAAFLASGYATKTIDDQQNKTALYVYDLESSGTLIKKID
                     VPGGKGGLSSPTLVDKDLDGTVDIAYAGDRGGKMYRFDLSSQSPDQWTVRPIFEGTKP
                     ITSAPAISQLKDKRVVIFGTGSDLSEDDVLSTSEQYIYGIFDDDTATTGSVNFSGLGG
                     GLLEQELKQEGKTLFLTDYKRSDGSGNKGWVVKLNGGQRVTVKPTVVLRTAFVTIRKY
                     NDGGCGAETAILGINTADGGKLTKKSARPIVPEANQAVAQYSGHKQTAKGKSIPIGCM
                     WKNNETVCPNGYVYDKPVNVRYLDEKKTDGFSTTADGDAGGSGIDPAGKRSGKNNRCF
                     SQKGVRTLLMNDLDSLDITG"
BASE COUNT          382 a          361 c          388 g          281 t
ORIGIN      
        1 cagttatatc cccggcacga tgccgcgcaa ggatattgaa aacaacgact ccacccttgc
       61 caaagagctg cgcgcctttg ccgaaaaagg ctatgtgggc gaccgctacg gcgtggacgg
      121 cggctttgtc ttgcgccaag tcgaactgag cgggcaaaaa cacgtgttta tgttcggcgc
      181 gatgggcttt ggcggcagag gtgcgtatgc cttggatttg accaaagccg acggcaatga
      241 cccgacaaaa gcctctttgt ttgatgtaaa agataacggc aataatggca ataacggcaa
      301 taatagcgtg caattaggct acaccgtcgg cacgccgcaa atcggcaaaa cccacaacgg
      361 caaatacgcc gctttcctcg cctccggtta tgcgactaaa accattgacg accaacaaaa
      421 taaaaccgcg ctgtatgtgt atgatttgga aagcagcggt acgctgatta aaaaaatcga
      481 tgtacccggc ggcaagggcg ggctttcgtc ccccacgctg gtggataaag atttggacgg
      541 cacggtcgat atcgcctatg ccggcgatcg cggcgggaaa atgtaccgct ttgatttgag
      601 cagtcaaagt cctgatcaat ggactgtacg ccctattttt gaaggcacaa aaccgattac
      661 ttccgcgccc gccatttccc aactgaaaga caaacgcgtg gttatcttcg gcacgggcag
      721 tgatttgagt gaggatgatg tactcagtac gagcgaacaa tatatttacg gtatcttcga
      781 cgacgatacg gcgacgacgg gtagtgtaaa cttcagcggt ttgggaggcg ggctgctcga
      841 gcaagagctt aagcaggagg gtaaaacctt attcctgacc gattacaagc gatccgacgg
      901 atcgggcaat aagggctggg tagtgaaatt gaatggcgga cagcgcgtta ccgtcaaacc
      961 gaccgtggta ttgcgtaccg cctttgtaac catccgcaaa tataacgacg gcggctgcgg
     1021 cgcggaaacc gccattttgg gcatcaatac cgccgacggc ggcaagctga ccaagaaaag
     1081 cgcgcgcccg attgtgccgg aagccaatca ggctgtcgcg caatattccg gccataagca
     1141 aaccgccaaa ggcaaatcca tccctatagg ttgtatgtgg aaaaacaatg aaaccgtctg
     1201 cccgaacgga tatgtttacg acaaaccggt taatgtgcgt tatctggatg aaaagaaaac
     1261 agacggattt tcaacaacgg cagacggcga tgcgggcggc agcggtatag accccgccgg
     1321 caagcgttcc ggcaaaaaca accgctgctt ctcccaaaaa ggggtgcgca ccctgctgat
     1381 gaacgatttg gacagcttgg acattaccgg cc
//
LOCUS       DAWFJR010000174         1387 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWFJR010000174 DAWFJR010000000
VERSION     DAWFJR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.174"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..621)
                     /locus_tag="WGR04_002072"
     CDS             complement(<1..621)
                     /locus_tag="WGR04_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0795549.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVFEGPVEQDESDFGGRRKGRRGRGAAGKVVVFGILKHNGRV
                     YTVIVDNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLDVSGFIHYRINHSKEFADRQ
                     NHINGIENFWNQAKRVLRKYNGIDRKSFPLFLKECEFRFNFGTPSQQ"
     gene            complement(741..947)
                     /locus_tag="WGR04_002073"
     CDS             complement(741..947)
                     /locus_tag="WGR04_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795550.1"
                     /translation="MFDGAKVVRRMEGNLWVFGMVALEKTCFKGQMPSENRFQTAFSV
                     YLKQTGKGSNILQSSYSHKRFIVN"
BASE COUNT          365 a          352 c          273 g          397 t
ORIGIN      
        1 ctgttgagac ggtgtgccga agttaaatcg aaattcgcat tctttcaaga acagcgggaa
       61 agatttacga tcgattccgt tgtattttcg caagacgcgt tttgcctgat tccaaaagtt
      121 ctcaatgccg ttaatgtggt tctgacggtc tgcaaattcc ttggaatggt tgatgcggta
      181 atggataaaa ccgctcacgt ccaacttgtc gcagctgctc agactatcgg tataaacaat
      241 actgtccggc atgattttct ttttgatgac agggagtaac gtttcagatt tggcattatc
      301 tacgataacg gtatagaccc gtccgttgtg tttcagaatg ccgaagacaa ccacttttcc
      361 tgccgcaccc cgaccacgtc tgcctttacg ccgtccgccg aaatcgcttt cgtcctgctc
      421 gacagggccc tcaaaaacct catcggcagc caaggccaaa tgatggttga taaccgtgcg
      481 gattttacgg tagaacagtg ctgccgaatt gggatggata cccaaaatat cggcggcaga
      541 acgggcggta acttccagta caaaaaaacg gagcagttct ttctgtactt ttttctttaa
      601 tttgcagtgc gttatcttca tatttcgagg gtaacatatc tgctaatcta gtacagcccc
      661 tagtttaaag ctttgtttct taagtccgca gagtatgcca tggttagacc ttcaaagttg
      721 agtattgtac tattttgttt ttaattaact ataaaacgct tgtgtgaata ggaagactgc
      781 agaatattgc tgccttttcc tgtttgcttt aaataaacag aaaatgccgt ctgaaaccga
      841 ttttcagacg gcatttgtcc cttaaaacac gttttttcaa gcgcgaccat accaaacacc
      901 cacaggttgc cttccatcct tcgtacaacc tttgctccat caaacatcca cttcctaaat
      961 actgatgtct gcttaggttg aatcggcatt cgtacgatta cggcacaccg cctaatctgt
     1021 acctgcgtct gttgcgccca ataccaaagg ccacctgaaa atctcccaaa cttttcaggt
     1081 agccttatca cattttcgac tgtcaccaca cgcccaaaga ccgcctgaaa ccctttttca
     1141 gacgttccgc tccgataccc cccaacaaaa cgccgcccga acacatgttc agacggcatt
     1201 ttctttgcgt cgattcattg cggtgtctac ctgctttaag gcggacaaag gtgcggaaac
     1261 ggtaaacaac gatacagaac ataatgctac atattttagc catttccctt ccaaacaaaa
     1321 aacaaaaaag caccggcggc agccgatgcc ccttccttta caggtttccc tattttattt
     1381 tttagct
//
LOCUS       DAWFJR010000175         1380 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWFJR010000175 DAWFJR010000000
VERSION     DAWFJR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.175"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..600
                     /locus_tag="WGR04_002074"
     CDS             <1..600
                     /locus_tag="WGR04_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HEZ0795551.1"
                     /translation="SARQWADAHPNITATAQTALAVAEAAGTVWGGKKVELNPTKWDW
                     VKNTDYKTPAARPMQTLDGEMAGGNKPPKSITSGGKANAATYPQLVNQLTGQNLKNIA
                     AQDPRLSLALHKSEKNFPIGTATYEEADRLGKIWVGEGARQTSGGGWLSIDGTRQYRP
                     PTEKNSQFATTGIQANFETYTIDSNGKRNKIKNGHLNIR"
     gene            605..964
                     /locus_tag="WGR04_002075"
     CDS             605..964
                     /locus_tag="WGR04_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0795552.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            1041..1253
                     /locus_tag="WGR04_002076"
     CDS             1041..1253
                     /locus_tag="WGR04_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795553.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
BASE COUNT          504 a          197 c          278 g          401 t
ORIGIN      
        1 tccgccagac aatgggctga tgcccatccg aatataactg caacagccca aactgccctt
       61 gccgtagcag aggccgcagg tacggtttgg ggcggtaaaa aagtagaact taacccgact
      121 aaatgggatt gggttaaaaa taccgattat aaaacacctg ctgcccgacc tatgcagact
      181 ttagatgggg aaatggccgg tgggaataaa ccgcctaaat ctataacgtc tggaggaaaa
      241 gccaatgctg caacttatcc tcaattagtt aatcaattaa ctgggcaaaa cttaaaaaac
      301 attgcggctc aagatccaag attgagtcta gctcttcata agagtgaaaa aaattttcca
      361 ataggaactg caacttatga agaggcagat aggctaggta aaatttgggt tggtgagggt
      421 gcaagacaaa ctagtggagg cggatggtta agtatagatg gcactcgaca atatcggcca
      481 ccaacagaaa aaaattcaca atttgcaact acaggtattc aagcaaattt tgaaacttat
      541 actattgatt caaatggaaa aaggaataaa attaaaaatg gacatttaaa tattaggtaa
      601 ttatatgaga ttaaaattaa aaaattatga ttgttctgac tttgatttgg aagatttccc
      661 acaagacaag ttagaaaatt tttgtatact tttgacatta tcaataggtt ttgatgaaag
      721 taatggtgct gattattttt atgtttatat atatagcact gaatggttac tttcaaatat
      781 acatagaccg atgtcattaa aaaactcaat tgttacaaac cgttttaata ttgaacacat
      841 attaaaattg ataaatgata tattggaaat atgtaattca acgtctgaag ataagtctat
      901 atcaaatttg gcaaaatatt ttgactggga atttgacgat tataatctaa atatccaaga
      961 ctaaagatgg tgggacagca tttagaccaa aaacaggcaa acggtatttt gatgacgaat
     1021 taaaataaaa ggaattattc atgaaattaa ttaattttga agagagttca gttaaaatta
     1081 gtctcgataa agatgagttg tatattattc aagcaattgt aggagaaatt tattcaggcg
     1141 tatgcgtgga ttgtagagat tttgagataa ttcatggtgt tgaaaaaata aagttctttc
     1201 tttggataaa gaacttaaaa aaatatatga tacttgggat aaatgttagc taggtaaggt
     1261 gggcacttgt tgcccacctt ttgccgtata ttaaaaatcc ttttcaggct gcctgaaact
     1321 gggtgggcat caatgccaat cctaactgtg ctgatgaagc aggaaaactg atatgggaaa
//
LOCUS       DAWFJR010000176         1332 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWFJR010000176 DAWFJR010000000
VERSION     DAWFJR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.176"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            61..1068
                     /locus_tag="WGR04_002077"
     CDS             61..1068
                     /locus_tag="WGR04_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0795554.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPLLSMLKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSELLELINRQLTEKGLKIEKASAAVVDATIIQTAGSKQRQAIE
                     VDEEGQINGQTTPSKDKDARWIKKNGLYKLGYKQHTRTDAEGYIEKLHITLANAHECK
                     HLSPLLEGLPKGTTVYADKGYDSAENRQHLKEHQLPDGIMRKACRNRPLTETQTKRNR
                     YLSKTRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAA
                     A"
BASE COUNT          372 a          385 c          317 g          258 t
ORIGIN      
        1 acctttgcaa aatcccccaa aatcccctaa attcccacca agacatttag gagatttctc
       61 atgagcacct tcttccagca aaccgcccaa gccatgattg ctaaacacat cgaccgtttc
      121 ccgctattga agttggacca ggtgattgat tggcagccga tcgaacagta cctgaaccgt
      181 caaaaaaccc gttaccttag agaccaccgc ggccgtcccg cctatcccct gctgtccatg
      241 ctcaaagccg tcctgctcgg acaatggcac agcctctccg atcccgaact cgaacacagc
      301 ctcatcaccc gcatcgactt caacctgttt tgtcgttttg acgaactgag catccccgat
      361 tacagcacct tatgccgcta ccgcaactgg ctggcgcaag acgacaccct gtccgaactg
      421 ttggaactga ttaaccgcca actgaccgaa aaaggtttaa aaatagagaa agcatccgcc
      481 gctgtcgttg acgccaccat tattcagacc gccggcagca aacagcgcca ggccatagaa
      541 gtcgatgaag aaggacaaat caacggccaa accacaccga gtaaagacaa agatgcccgc
      601 tggataaaga aaaacggcct ctacaaactc ggttacaaac aacatacccg taccgatgcg
      661 gaaggctata tcgagaaact gcacatcacc ctcgccaatg cccatgagtg caaacacctg
      721 tcgcctttgc tggaaggact gcccaaaggt acgaccgtct atgccgacaa aggctacgac
      781 agtgcggaaa accggcaaca tctgaaagaa catcagttac cggacggcat tatgcgcaaa
      841 gcctgccgca accgtccgct gacggaaacg caaaccaaac gcaaccggta tttgtcgaag
      901 acccgttatg tggtcgaaca gagcttcggt acgctgcacc gtaaattccg ctatgcgcgg
      961 gcagcctatt tcggactgat taaagtgagt gcgcaaagcc acctgaaggc gatgtgtttg
     1021 aacctgttga aagcggccaa caggctaagt gcgcccgctg ccgcctaaaa ggcgaccgga
     1081 tgcctgatta tcgggtatcc gggcaggatt aagggggtat ttgggtagaa ttaggcggta
     1141 tttggggcga aaatagacga aaacctgtgt tggggtttcg gctgtcggga gggaaaggaa
     1201 ttttgcaaag gtctccgacc gtttcccgct attgaagttg gaccaggtga ttgattggca
     1261 gccgatcgaa caatacctga accgtcaaaa aaaccgttac cttagagacc accgcggccg
     1321 tcccgcctac cc
//
LOCUS       DAWFJR010000177         1307 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWFJR010000177 DAWFJR010000000
VERSION     DAWFJR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.177"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            27..764
                     /locus_tag="WGR04_002078"
     CDS             27..764
                     /locus_tag="WGR04_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0795555.1"
                     /translation="MVKTADGYKAIAHIRAGESVLSKDEASGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGSRLLSENGRTQTVRNIVV
                     KPKPLKAYNLTVADWHTYFVKGDKAETEGVWVHNDCPYGGSNNLEKAKLRAERLSKND
                     RAGKDFTKAGKEAVIDLNRIQNNGQVKCANCGIETIPAKQSIKNISPTSNERQVDHVI
                     PKSKGGQGTPKNGQVLCRGCNIKKSNK"
     gene            761..1180
                     /locus_tag="WGR04_002079"
     CDS             761..1180
                     /locus_tag="WGR04_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HEZ0795556.1"
                     /translation="MKSKLTVVYYDLESNIAEEILSGNIMPDGNFLIQEIPLFAPNLA
                     LNDIVAIEREDKMLFFDHLIKASGNTTINIVVLDHFPKDLLAAIEEHSGKIRKNGENY
                     LSVNFPPKKYNSDLKGILNRYEEANILSYREACLGFS"
BASE COUNT          447 a          258 c          272 g          330 t
ORIGIN      
        1 acacctactc cttccacggc agcaccttgg tcaaaacggc agacggctac aaagccattg
       61 cccatatccg agccggcgag agcgtcctct ccaaggacga ggcaagtgga aaaacgggat
      121 acaaacccgt taccgcccga tacggcaatc cctatcaaga aaccgtttac attgaaattt
      181 cagacggcat cggcaacagt caaaccctga tttccaaccg catccatccg ttttattcgg
      241 acggcaaatg gattaaggcg gaagacttaa aggcgggaag cagactgtta tccgaaaacg
      301 gcagaaccca aaccgtccgc aacatcgttg tcaaaccaaa accactcaaa gcctacaatt
      361 tgaccgttgc cgattggcat acttactttg tcaaaggcga taaggcggaa acggaagggg
      421 tttgggttca taatgattgt ccgtatggcg gttcaaataa tttagagaaa gcaaaacttc
      481 gcgcagaacg gcttagtaaa aacgatcggg caggaaaaga ctttactaaa gcaggaaaag
      541 aagccgttat tgatttgaat aggattcaaa ataatggtca agttaagtgc gctaattgcg
      601 gtattgaaac aattccagcc aaacaatcta taaaaaatat ttctcctact tcaaatgaaa
      661 gacaagtaga tcatgtcatc cctaaatcta agggtggtca aggtacacct aaaaacgggc
      721 aggtattatg cagaggctgc aacattaaga agagtaacaa atgaaatcga aacttactgt
      781 cgtctattac gatttggaaa gcaatattgc agaagaaata ctgtcaggaa acataatgcc
      841 tgatggaaat tttcttattc aagagattcc tcttttcgca ccgaatttgg ctttaaacga
      901 cattgttgcc atagaacgtg aggataagat gctgtttttc gaccacttga taaaagcttc
      961 aggaaatacc acgataaaca tcgttgtttt ggatcatttc ccaaaggatt tattggcagc
     1021 catagaagaa cacagtggta aaatcagaaa aaatggagag aattatttat cggttaattt
     1081 tccgcccaaa aaatataatt ctgatttaaa aggaatttta aatagatatg aggaagcaaa
     1141 tatcctcagc tacagggaag cttgtttggg cttctcttaa tcgatctata agtaagaatg
     1201 gattacgtta gattgtaaac ctaacgatta aaaggtcgtc tgaaaccgtg tttaattttc
     1261 agacgaccta tttccttcat ttgaaacagg atattgggaa ttaagtt
//
LOCUS       DAWFJR010000178         1300 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWFJR010000178 DAWFJR010000000
VERSION     DAWFJR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.178"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..456)
                     /locus_tag="WGR04_002080"
     CDS             complement(<1..456)
                     /locus_tag="WGR04_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795557.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            complement(1020..1223)
                     /locus_tag="WGR04_002081"
     CDS             complement(1020..1223)
                     /locus_tag="WGR04_002081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795558.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAGAS
                     AIANASIGYQWTVNLSEPKEGAK"
BASE COUNT          456 a          238 c          142 g          464 t
ORIGIN      
        1 gtctagagtc tgtatattac atttattttt agggtctgct agccaatttc ttgttccctt
       61 cattatttta tcttctgaaa gaaaattatt tttttccatg ctattaatat taatgatatg
      121 atttttattt aaaataattt ttccatataa aatttccggt ttgtaatagc aagagtctga
      181 agcaaatcct tgcccgtcaa tataagaaaa ataaatgtta tcatcctcct tataatattc
      241 taaaaccaat ccggatatta ttttatcaaa attcccttca tttccatctt tcatgactaa
      301 aatataatta gcaggccaac ctttatcgta aacatattca aactgatagt tcccgaaact
      361 ctcgatatcc gaactaaaaa agaagaaaag caaattaata gccaatacaa aaacaaataa
      421 gaacaaaaat agcaaaattt tcaacttagt taacaatatt tacctctcct ttaaattcaa
      481 tcctgaaagg taccccttac cctgggctac caattatagt ttccatattt ctaaatattg
      541 tttttacatt acttttttct ccccccaaag gaatgcattt taaaatcatg cttttcaggt
      601 gctaatcgat acttaccatt aacatcttta atcacagata tatttccatg tatagcccaa
      661 cgtgaaaaat ctgagtatta tatacagtta taccttactg aaacaaagct agctgtacat
      721 atctgtacca tatttttact acaaacaaaa aatatttata attattaagg ataagataaa
      781 aacccccaac gccagattaa ttcttaataa tttcaaataa aaaatatttg agattttttc
      841 ttttccaaat aatatattga aatatttaaa gttaaggtag cttgatttaa gtagaaaatc
      901 aaaaaaacca ttaattaatt tttcttcttt aaatatttta tatgatatta taaaattaaa
      961 taacattgcg aaaacatatg aaatcatcaa aataccaaat atatagtggg aatgcattat
     1021 tatttagctc cttcttttgg ctctgacaaa ttgacagtcc attgatatcc aatgcttgca
     1081 tttgcaattg ctgacgcacc tgcccctgtt ttccctgtta gtatcaatgg ttcttttctt
     1141 gcaagatgtg cacctgcaca accacctaat actaaaccac cacctattgt aaatccttcc
     1201 tgttgtatct ctaatgataa cattttcttc ttgtgccgcc cttaatacag attgtggaat
     1261 tggattgacc tgagcaggaa cttctaaaat aagttttcct
//
LOCUS       DAWFJR010000179         1099 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWFJR010000179 DAWFJR010000000
VERSION     DAWFJR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.179"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..194
                     /locus_tag="WGR04_002082"
     CDS             <1..194
                     /locus_tag="WGR04_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0795559.1"
                     /translation="NNRVNQAEEDNYSTSVGSCINGFTVQYYPFIREKQQLTQQELVG
                     YHQQVEQLVQSFVNNSSKK"
     gene            211..>1099
                     /locus_tag="WGR04_002083"
     CDS             211..>1099
                     /locus_tag="WGR04_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795560.1"
                     /translation="MNEGEVVLTPEQIQTLRGYASRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFRQYARLINQNNGR
                     LPNTSEIERSYYKAVTDNGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQA
                     VNNPNGSERDNRKQLISALDKGFDGSFKEKHFTFLQSVMMDVTKLGVEYTIDGWQKIG
                     GWGNGIINDLYKSVVKREWTGIFEIVNNNIKQGNEAFKNEINSLVHDMKAAGKEFGDD
                     LNTQWNNLTQAAEIIYNDIV"
BASE COUNT          371 a          149 c          264 g          315 t
ORIGIN      
        1 ggaataatcg agtaaaccag gctgaagaag ataattatag cactagcgta ggttcctgta
       61 ttaacggttt cacggtacag tattacccgt ttattcggga aaagcagcag ctcacacagc
      121 aggagttggt aggttatcac caacaagtag agcaattggt acagagtttt gtaaacaatt
      181 caagtaaaaa ataatttaaa ggatcttatt atgaatgagg gtgaagttgt tttaacacca
      241 gaacaaatcc aaaccttgcg tggttatgct tcccgtggcg atacctatgg cggttggcgt
      301 tatttggcta atttgggtga ccgttatgcg gatgatgctg ctgcaattgt cggtaaggat
      361 gcaaacttaa atggtttgaa tttatggatg aaaaaaggtg tggaaaacct atgggatgat
      421 acggtcggta aaaagacccg tttagagaaa tttgatcggg ttgcactgca acatttcagg
      481 caatatgcgc gtctaattaa tcaaaataat ggtagattac ccaatactag tgaaattgag
      541 agaagttact ataaagccgt taccgataat ggcgtttctt ccagtgcagc tattgattta
      601 gttattaatc gttcacttcc ggatatggcg gatggttatt gggcattagg tttggggata
      661 gaagccgaac gtatccacaa tgagcaagca gtaaataatc cgaacggtag cgaaagggat
      721 aatagaaagc agttaatatc tgctttagat aaaggatttg atggatcttt taaagagaag
      781 cattttactt ttttacaatc tgtgatgatg gatgtaacaa agttaggtgt tgaatataca
      841 atagatggtt ggcaaaaaat tggaggttgg ggtaatggga taatcaatga tttatataaa
      901 agtgttgtaa aaagagagtg gactggaata tttgagatcg ttaataataa catcaagcaa
      961 ggaaatgaag cttttaaaaa tgaaatcaat agcttggttc atgatatgaa agctgctggc
     1021 aaggaatttg gagatgactt aaatacacag tggaataatc tcactcaggc tgccgaaata
     1081 atctataatg acatagtag
//
LOCUS       DAWFJR010000180         1028 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWFJR010000180 DAWFJR010000000
VERSION     DAWFJR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.180"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(9..200)
                     /locus_tag="WGR04_002084"
     CDS             complement(9..200)
                     /locus_tag="WGR04_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795561.1"
                     /translation="MDFQFFVETIRRQEKKLKHGVYRLFEIYSWLEDGKATKARIRYF
                     VKIEVDKQILRRIHRGIAD"
     gene            329..889
                     /locus_tag="WGR04_002085"
     CDS             329..889
                     /locus_tag="WGR04_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HEZ0795562.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINLPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT          296 a          284 c          206 g          242 t
ORIGIN      
        1 ggtttttgtt aatccgctat accacgatga atccttcgca atatctgttt atcgacctca
       61 attttgacaa aataccggat acgcgccttt gttgcttttc catcttccaa ccaactgtaa
      121 atctcaaaca gccggtacac gccatgcttc agtttctttt cctgtcggcg gattgtttcg
      181 acaaagaatt gaaaatccat ttcatgcacc ttaaaattta atctgcattc aaaccttttc
      241 actttggaag caccatttat cggatgtccc ttcgcaataa acaaattttc ccgataccgc
      301 cgcccatttc aacccaaacc caaaacctat gaaaaacctc atcgccttca acaaacccta
      361 tggcgttatc tgccaatttt caccgcacga aaaacacaaa agcctcaaag actttatcaa
      421 tcttcccggc ttctaccccg ccggacggct cgacaccgac agcgaggggc tgctgctgct
      481 gaccgacgac ggcaggcttc aggcacaaat taccgacccc aaattcaaac accccaaaac
      541 ctactgggcg caactggagg gcgtacccga cgaaagccga ttggaaagcc taagaaaagg
      601 gatagactta ggcggtttcg ttacccgtcc ggcaagcatc cgcatcttga aacacggaga
      661 agcagattcg ttatgggagc gcatcccgcc gatacgcgtc cgcaaaaccg ttcccgattt
      721 ttggattgaa attaccattt ctgagggcaa aaaccgccaa gtcaggcgaa tgaccgccaa
      781 ggcgggctat ccctgcctgc gtctgatcag agtggcaagc ggcagactga aactgtttga
      841 tttggattta aaacccgggg aatgggcata cgccccgttt aaaccataat cacgtttatc
      901 tcatcatttc cacaaaagtg ggaatccgga attttatagt ggattaacaa aaatcaggac
      961 aaggcgacga agccgcagac agtacaaata gtacgaaacc gattcactcg gtgcttcagc
     1021 accttaga
//
LOCUS       DAWFJR010000181          980 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWFJR010000181 DAWFJR010000000
VERSION     DAWFJR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.181"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..183
                     /locus_tag="WGR04_002086"
     CDS             <1..183
                     /locus_tag="WGR04_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795563.1"
                     /translation="HAVGMALAKKQAEVSRLKTENKKEIENVLTQDRKNAGGGCIDGF
                     GHHGLQLYNRALGYGN"
     gene            203..385
                     /locus_tag="WGR04_002087"
     CDS             203..385
                     /locus_tag="WGR04_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795564.1"
                     /translation="MPTPPAALMVAPVRPNPPKDGKTVTLLEHAAEFGGYVSELENQN
                     QAWRDWAGNHSRKVGN"
     gene            complement(430..600)
                     /locus_tag="WGR04_002088"
     CDS             complement(430..600)
                     /locus_tag="WGR04_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ0795565.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            613..831
                     /locus_tag="WGR04_002089"
     CDS             613..831
                     /locus_tag="WGR04_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795566.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
BASE COUNT          266 a          234 c          250 g          230 t
ORIGIN      
        1 cacgctgtcg gcatggcttt ggcgaaaaaa caggcggaag tcagccgtct gaaaacggaa
       61 aataaaaagg aaatcgaaaa tgtccttact caagaccgta aaaatgcagg cggcggttgt
      121 attgacggct ttggccatca cggcttgcag ctctacaacc gcgccctcgg ctacggaaat
      181 taaggttgtc gaaaaggcgg tcatgccgac accgcctgcc gcattgatgg ttgcgccggt
      241 acgcccgaat ccgccgaaag acggcaagac agtaacgctg ttggaacacg ccgctgagtt
      301 tggcggctat gtttcggaac tggagaacca aaatcaggct tggcgcgact gggcgggcaa
      361 tcactcccgc aaagtcggaa actgacaaaa aagcccgcgt agggcgcggg ctgagggtga
      421 aagcggattt tatacctctt ttacaggggt agcggcggta gtgcttttca gcaaatcgac
      481 tgcgtgctga cagttttgct tgctggtgta gccttcgccc tgagcgatga tttcatggtt
      541 ggctgctttc aaacgccaac ggtattcgcc ttttgcgtct ttatagattt caaaatacat
      601 aaggtttctc ctatgaatga gtacacgttt tcttaccgct ttaacggcaa gtcctggtca
      661 ttgagcattt gggcggacaa ccctgaagaa gccagggcga aatttcgggc tgcacgagaa
      721 aatgcgcact atgacggcga agttgtagca aaggtttata catttgtaaa tatttcgtgg
      781 gttaagaaat tgtacaagcg gacaaaatat ttaatgggta tcaaagaatg acctaccgag
      841 acctttgcaa aattccccaa aatcccctaa attcccacca agacatttag gagatttctc
      901 atgagcacct tcttccggca aaccgcacaa gccatgattg ctaaacacat cgaccgcttc
      961 ccactattga agttggacca
//
LOCUS       DAWFJR010000182          960 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWFJR010000182 DAWFJR010000000
VERSION     DAWFJR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.182"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            9..>960
                     /locus_tag="WGR04_002090"
     CDS             9..>960
                     /locus_tag="WGR04_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEZ0795567.1"
                     /translation="MLEKQHFPRFVIGESLLPHCMEMLEEAGFADAVRAEPGFQLKNG
                     AAFSWGSRYTEFDFTDKFSDGPGTTYQVRRAVFDKILIEEAAKQGVEVRFGHGVTAFD
                     NSGDFVRLNIETDTGKRYELTAKFVLDASGYGRVLPRLLNLETPSHLPPRQAHFTHID
                     DNITNPKFDRNKILITTYPQHRDVWIWLIPFGDNRCSVGVVGTPDKLAGESETVLKKF
                     VYECPMLNEILDKAVWENDFPFRSIQGYSANVKSLHGRHFALLGNAAEFLDPVFSSGV
                     TIALHSAKLAADLLAKQLKGEAADWQTEFAEPLMIGVDAFR"
BASE COUNT          224 a          288 c          250 g          198 t
ORIGIN      
        1 aagtctgcgt gttggaaaaa cagcactttc cgcgcttcgt catcggcgaa agcctgctgc
       61 cgcactgtat ggaaatgctg gaagaagccg gatttgccga tgccgtgcgc gccgagcccg
      121 gctttcagtt gaaaaacggc gcggcgtttt cgtggggcag ccgttatacc gaattcgatt
      181 tcaccgataa attttcagac ggccccggca cgacttatca agtgcgccgc gccgtgttcg
      241 ataaaatcct gattgaagaa gccgccaaac aaggcgttga agtacgtttc gggcacggcg
      301 taaccgcgtt cgacaacagc ggcgattttg tccgcctgaa catcgaaacc gacaccggca
      361 agcgctatga actgaccgcg aaattcgtct tggacgcaag cggttacgga cgcgtgctgc
      421 cgcgcctgct aaacttggaa acgccctcgc acctgccgcc gcgccaagcg catttcacgc
      481 acatcgacga caacatcacc aacccgaaat tcgaccgcaa caaaatcctg attaccacct
      541 atccgcaaca ccgcgacgtg tggatttggc tgattccctt cggcgacaac cgctgttccg
      601 tcggcgtggt cggcacgccc gacaaacttg ccggcgaatc ggaaacagtg ttgaaaaaat
      661 ttgtttacga atgcccgatg ctgaacgaaa ttttggacaa agccgtttgg gaaaacgatt
      721 ttccgttccg ctccatccaa ggctattccg ccaatgtgaa atcgctgcac ggcaggcatt
      781 tcgcgctatt gggcaatgcc gccgagttcc tcgatcccgt gttctcgtcg ggcgtaacca
      841 tcgcgctgca ctccgccaaa cttgccgccg atctgttggc aaaacaactc aaaggcgaag
      901 ccgccgattg gcaaaccgaa tttgccgaac ccttgatgat cggcgtggac gcgttccgca
//
LOCUS       DAWFJR010000183          919 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWFJR010000183 DAWFJR010000000
VERSION     DAWFJR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.183"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..131)
                     /locus_tag="WGR04_002091"
     CDS             complement(<1..131)
                     /locus_tag="WGR04_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0795568.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     "
     gene            780..>919
                     /locus_tag="WGR04_002092"
     CDS             780..>919
                     /locus_tag="WGR04_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0795569.1"
                     /translation="MKYRRFYRNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRE
                     RYP"
BASE COUNT          234 a          209 c          230 g          246 t
ORIGIN      
        1 cgggttcttt gacgattcag gtactgttcg atcggctgcc aatcaattac ctgatccaac
       61 ttcaatagtg ggaagcggtc gatgtgtttg gcaatcatgg cttgtgcggt ttgccggaag
      121 aaggtgctca tggaaaatcc cctaaatggc ttggtgggaa tttaggggat tttggggaat
      181 tttgcaaagg tctcaaaccg tttttctcga taagtttccg tgccgacaga cctagattcc
      241 cactttcgtg ggaatgacgg tggaaaagtt gccgtgattt cggataaatt ttcgtaacgc
      301 ataatttccg ttttacccga taaatgcccg caatctcaaa tcccgtcatt ccccaaaaac
      361 aaaaaatcaa aaacagaaat atcgtcattc ccgcgcaggc gggaatctag gtctgtcggt
      421 gcggaaacgt atcggataaa acggtttctt cagattttac gttctggatt cccactttcg
      481 tgggaatgac gggatgtagg ttcgtaggaa tgacgtggtg caggtttccg tgcggatgga
      541 ttcgtcattc ccgcgcaggc gggaatccag ccccctgacg cggcgggaat ctatcggaaa
      601 tgactgaaac cccgcgttct agattcccac ttccgtggga atgacggttc agcaagcgta
      661 ggtcggatac ttgtatccga caaaaccttt aacattccca tcattgcaat ccattgcagc
      721 aatgcccaaa atgtcgaatt caagaatccg acctacaaaa tcattccgag cataatacta
      781 tgaaataccg tcgtttttac cgcaatggcg gcacttactt ttttacggtt gtaaccaata
      841 aacggcagaa gattttgacc gatgatgcgg tgcgtttggc tttacggcag gcggtaatgg
      901 cggtgcgcga acggtatcc
//
LOCUS       DAWFJR010000184          905 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWFJR010000184 DAWFJR010000000
VERSION     DAWFJR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.184"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..215
                     /locus_tag="WGR04_002093"
     CDS             <1..215
                     /locus_tag="WGR04_002093"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795570.1"
                     /translation="DAGDVYCVYNIRLKQYTACQVTRVEEHGGKKTHATLLALDWQGN
                     KPLGAEELADLKPLYKDLKNNIGNIV"
     gene            546..>905
                     /locus_tag="WGR04_002094"
     CDS             546..>905
                     /locus_tag="WGR04_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795571.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQISEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLY"
BASE COUNT          281 a          146 c          212 g          266 t
ORIGIN      
        1 ctgatgccgg agacgtatat tgcgtctata acatcagact gaagcagtac actgcctgcc
       61 aggttacccg agttgaagaa cacggtggca aaaaaacaca tgcgaccctg ctggctttgg
      121 actggcaggg caacaaaccg cttggggcgg aggagctggc ggatttgaaa ccgctttaca
      181 aagacttaaa gaataatatt ggaaatattg tatgaacaaa aaattaaact atatttttat
      241 gttggactgt ttagggttgg tgatattgtt tacttgtata atagctactt ttgaaagaga
      301 ttatggattt aaaattttta ctaattctaa gaggcctgaa ttttattatt ggattggaat
      361 gttttattat ggaattattt cttgctggtt tgattatcaa ttaatttcaa caaaggcgaa
      421 ttcgtataaa agaaaagtta aacaatataa aattttttca gtaatatttt cagttttgat
      481 atttatttct actatagtaa aactttaaat tttggagcaa aaatttatga gcgattcaat
      541 tgaatatgta ttgggaacgc ggtctgcaca tgtataaggc aagtgccgtc gtgccgacgg
      601 gatatgtacg ggttgggaat accgcgccgc tggtcggcga agacacgcaa cggtatgcct
      661 ctttttgggg cgacggctac gacgtgtacc gtcagttgag atggcagcag atatccgaaa
      721 aacagagaaa ggcattcaaa aaagccgcta aaagcaaaaa gaccgtgatg tttgccggac
      781 gggaatacgg catatccaaa cagaatttga gcgatgtttg ggatgatttt gaagacgcga
      841 tggaactgaa ggcgtttccc tgcctgtctt cgctgtttct gaccaaatgg cataaaaatc
      901 tatat
//
LOCUS       DAWFJR010000185          899 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWFJR010000185 DAWFJR010000000
VERSION     DAWFJR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.185"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(48..827)
                     /locus_tag="WGR04_002095"
     CDS             complement(48..827)
                     /locus_tag="WGR04_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ0795572.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT          218 a          289 c          178 g          214 t
ORIGIN      
        1 ttcactatac cgatacatcc gaatatgcca gaccgtctaa acggtattca tacctcccaa
       61 tcctgcacgc gcttcaaatc gttttgcgcc aaagtatccg cgtggcggtt acggctctga
      121 tattccctgt ctttcatgat gctgcttgcc acataattca aatgtgcctt tgccgcctcc
      181 gaagcctcgc ccggccgacg gttcgatatc gcctcataca gcacgcggtg ctgcgccatc
      241 agtttcggac gcggatcttc ttcctgattc agataaataa ggctgctgcg cgtctgccgg
      301 tacagcattt tcaacaaacc gcccgacaaa tggctgaaca acaaattgtg cgccgcatcc
      361 gcaatcgtct gatggaagct gacatcggct tcgctctgat gttccagatt gccgctttcg
      421 cacgcctcct caaacttttc aagccaaaac ccaatccgct tcaaatcggc atccgtccgg
      481 cgttctgccg ccaatgccgc catacagccc tcgatgtggc agctgaaatc aaaaacatcc
      541 tgttcccaat tggaatgctt gcccaaaagc tcctgccaac tttgcaaaaa atcctgctgt
      601 ggcttgaccg aaacataata accgtctccc tgcctcgctt ccaaaacctg acgggcaacc
      661 aaaacattca atgccgacct gaccgacggg cgggacacgc cgaactcttc cgccaaaacg
      721 cgttcgggcg gaatcttgcc cccttccgcg taaacccctt ccgcaatgcg ctcctccaat
      781 accgacaata cctgatcgct gattttctga ggccttacca gtttcattac tcctccttta
      841 taaagattcc ctgcagaacc cttccgaaat atagtggatt aacaaaaatc aggacaagg
//
LOCUS       DAWFJR010000186          869 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWFJR010000186 DAWFJR010000000
VERSION     DAWFJR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.186"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            236..677
                     /locus_tag="WGR04_002096"
                     /pseudo
     CDS             236..677
                     /locus_tag="WGR04_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          264 a          249 c          181 g          175 t
ORIGIN      
        1 aaaaacagaa acctaaaatt cgtcattccc acgaaagtgg ggatccagaa cgtaaaatct
       61 aaagaaaccg ttttcccgat aagtttctgc accgacgggg ctggattccc gcctgcgcgg
      121 gaatgacgaa gctatccata cggaaatcta catcccgtca ttcccacgaa agtgggaatc
      181 cagaacgcaa aatctaaaga aaccgttttc ccgataagtt tctgcaccga caaggctgga
      241 ttcccgcctg cgcgggaatg acgaatccat ccttttggct gaaggtcaaa aatcagccgt
      301 caccgagtat tacctgaatc acggcatatg gcccggcgac aacacttctg ccggcgtggc
      361 atcttcttca acaatcaaag gcaaatatgt taaaagcgtt gaagtcaaaa acggcgtcgt
      421 taccgcccaa atggcttcaa gcaacgtaaa caacgaaatc aaaggcaaaa aactcttccc
      481 tgtgggccaa gcgtcaaaac ggttcggtaa aatggttctg cggacagccg gttacgcgca
      541 aagccaacaa cgccgacgac gtcgccgccg acggcaacga caaaatcgac accaagcacc
      601 tgccgtcaac ctgccgcgac gcttcatctg ccgtttgcat agaaacacca cctacggctt
      661 tctataaaaa tacctaaacc gtcattccca cgaacctaca tcccgtcatt cccacgaaag
      721 tgggaattca gaacgcaaaa tctaaagaaa ccgctttccc gataagtttc cgtaccgaca
      781 gacctagatt cccgcctgcg cgggaatgac ggcggagcgg tttctgttgc tcccgataaa
      841 ttcctaaaac ttaaaatttc atcattccc
//
LOCUS       DAWFJR010000187          822 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWFJR010000187 DAWFJR010000000
VERSION     DAWFJR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.187"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..407
                     /locus_tag="WGR04_002097"
     CDS             <1..407
                     /locus_tag="WGR04_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0795573.1"
                     /translation="SVEVKNGVVTATMASSNVNNEIKGKKLSLWAKRQDGSVKWFCGQ
                     PVARAANNTADDAVKADTAANDKKIDTKHLPSTCRDDSSTGCIETPRADFKHFQKISR
                     YRVLPESRHMAEKIRHSRAGGNLGLSVRKLIG"
     gene            454..801
                     /locus_tag="WGR04_002098"
     CDS             454..801
                     /locus_tag="WGR04_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0795574.1"
                     /translation="MTGFNDAAGVASSSDIKGKYVQSVTVAKGVITAEMKSSVVNKEI
                     QGKKLSLWAKRQAGSVKWFCGQPVTRDANNADTVAAAATKKIDTKHLPSTCRNESTAG
                     CIETPPTAFYKNT"
BASE COUNT          242 a          246 c          188 g          146 t
ORIGIN      
        1 aaagcgttga agtcaaaaac ggcgtcgtta ccgccacaat ggcttcaagc aacgtaaaca
       61 acgaaatcaa aggcaaaaaa ctctccctgt gggccaagcg tcaagacggt tcggtaaaat
      121 ggttctgcgg acagccggtt gcgcgcgccg ccaacaacac cgccgacgac gccgtcaaag
      181 ccgacaccgc cgccaacgac aagaagatcg acaccaagca cctgccgtca acctgccgcg
      241 atgattcatc taccggttgc atagaaacac cacgcgccga cttcaaacac ttccaaaaaa
      301 tcagccgtta ccgagtattg cccgaatcac ggcacatggc cgaaaaaatt cgtcattccc
      361 gcgcaggcgg gaatctaggt ctgtcggtgc ggaaacttat cgggtaaaaa ggtttcttga
      421 gattttacgt tctggattcc cactttcgtg ggaatgacgg gatttaatga tgccgccggc
      481 gtggcatcct cctccgacat caaaggcaaa tatgttcaaa gcgttacagt cgcaaaaggc
      541 gtcattaccg ccgaaatgaa atcaagcgtc gtaaacaaag aaatccaagg caaaaaactc
      601 tccctgtggg ccaagcgtca agccggttcg gtaaaatggt tctgcggaca gccggttacg
      661 cgcgacgcca acaacgccga caccgtcgcc gccgccgcca ccaagaagat cgacaccaag
      721 cacctgccgt caacctgccg caatgaatca actgccggtt gcatagaaac accacctacg
      781 gctttctata aaaataccta aaccgtcatt cccacgaacc ta
//
LOCUS       DAWFJR010000188          805 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWFJR010000188 DAWFJR010000000
VERSION     DAWFJR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.188"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..743)
                     /locus_tag="WGR04_002099"
     CDS             complement(join(<1..524,524..743))
                     /locus_tag="WGR04_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242698.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0795575.1"
                     /translation="MARTAITDNIWEQLQTTMKAHGCHQWKNDRTVMEAILWKLRTGA
                     PWRDIPIELGSWKTAYNRFNRWSKKGLWQKFFFDLRKEIDKEWVFIDGSYVRCHQHAS
                     GARRGFDRAIGQSRGGNTTKIHLCVDSHGNPLDFKVTGGNVHDGQVANDLIEVIQEAQ
                     YFIADKGYDSQEIRDKAIEHGMKAIIPKRKNAKKPNPDFDSYLYKLRHLVENAFARLK
                     QFRSIATRYEKLARNFKSMLYLACSIIHAK"
BASE COUNT          193 a          175 c          160 g          277 t
ORIGIN      
        1 cttagcatga ataatagagc aagccaagta aagcatcgat ttaaaattac gagctaattt
       61 ctcatagcga gtagcaatac tacgaaactg ctttaatcgt gcaaatgcat tctcgactaa
      121 gtggcgtaat ttataaaggt agctatcaaa atcagggtta ggtttcttgg catttttacg
      181 ctttggtata atagctttca taccgtgttc tatcgcttta tctctgattt cttgcgagtc
      241 ataccctttg tcagcgataa aatactgggc ttcttgtatg acttctatca agtcgtttgc
      301 aacttgaccg tcgtgcacgt tacccccagt gactttaaaa tcgagcggat ttccatgcga
      361 gtccacacat aggtgtattt ttgtcgtgtt tccgccacgg ctttgtccaa ttgctctatc
      421 gaaaccacgc cgagctccac ttgcatgttg atgacaccgt acataacttc cgtcgatgaa
      481 tacccattct ttgtcaattt cttttcgtag atcaaaaaaa aattctgcca caagcctttt
      541 ttagaccatc ggttaaagcg gttataagcg gttttccatg accctagctc aataggtatg
      601 tctcgccatg gtgcacctgt tcttagcttc catagtatgg cttccatcac ggtacggtcg
      661 ttcttccatt gatgacagcc gtgcgctttc atggttgttt gtaattgttc ccatatgttg
      721 tcagttattg cggttctcgc cattgtttgt gtttgcctat atttcgttgg aatatagggt
      781 taaaataggg ctcttttggg cttat
//
LOCUS       DAWFJR010000189          796 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWFJR010000189 DAWFJR010000000
VERSION     DAWFJR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.189"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            72..>796
                     /locus_tag="WGR04_002100"
     rRNA            72..>796
                     /locus_tag="WGR04_002100"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          211 a          159 c          255 g          171 t
ORIGIN      
        1 acaagataat gttttgaaca ttgtcctgtt ggtttctttg aagcagacca gaagttaaaa
       61 agttagagat tgaacataag agtttgatcc tggctcagat tgaacgctgg cggcatgctt
      121 tacacatgca agtcggacgg cagcacagag aagcttgctt cttgggtggc gagtggcgaa
      181 cgggtgagta acatatcgga acgtaccgag tagtggggga taactgatcg aaagatcagc
      241 taataccgca tacgtcttga gagagaaagc aggggacttt cgggccttgc gctattcgag
      301 cggccgatat ctgattagct agttggtggg gtaaaggcct accaaggcga cgatcagtag
      361 cgggtctgag aggatgatcc gccacactgg gactgagaca cggcccagac tcctacggga
      421 ggcagcagtg gggaattttg gacaatgggc gcaagcctga tccagccatg ccgcgtgtct
      481 gaagaaggcc ttcgggttgt aaaggacttt tgtcagggaa gaaaaggctg ttgctaatat
      541 cagcggctga tgacggtacc tgaagaataa gcaccggcta actacgtgcc agcagccgcg
      601 gtaatacgta gggtgcgagc gttaatcgga attactgggc gtaaagcggg cgcagacggt
      661 tacttaagca ggatgtgaaa tccccgggct caacccggga actgcgttct gaactgggtg
      721 actcgagtgt gtcagaggga ggtagaattc cacgtgtagc agtgaaatgc gtagagatgt
      781 ggaggaatac cgatgg
//
LOCUS       DAWFJR010000190          792 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWFJR010000190 DAWFJR010000000
VERSION     DAWFJR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.190"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
BASE COUNT          201 a          194 c          191 g          206 t
ORIGIN      
        1 tcgttcccac ggtcaggatt tcagattgcg gacatctgtc agaaaagaca aaaaaccttc
       61 cgccgttatt ccctacaggc gagaatccga tccgttgaaa ttcggttgtt ttaaataaat
      121 tcttacagct ttgattttct gtttttccga taacgccgta actttgaaac gcgaaagcgg
      181 taatccgatc cgttgggatt ttgcaacttc aaatcaatcc gcaaactgaa atcccgtcat
      241 tcccgcgcag tcgtgaatcc gaacgcgtcc gcacgaaaac ctgcatcccg tcattcccac
      301 ggaagtggga atctaggacg taaaatctca agaaaccgtt ttatccgata agtttccgca
      361 ccgacagacc tggattcccg cctgcgcggg aatgacgaaa tttcagcgag ccgtagggtg
      421 ggctgtaggg tgggcttcag cccaccaatt tcaccgcatc aaaggttttt gggaatacgg
      481 cggtttcggt tttcggtggg aatgggcgga atggataaca ttgacggagt tgggggaata
      541 ggtggaaacg gtgggattgg tgggctgaag cccaccctac agccagccct atgtccacgc
      601 tgcacatcct acgcccgcct tgcgtttctt ataagcagca ttttacgcaa tactttgcgt
      661 ccgctgaaaa aatttcaaac ggtctttttt atcaaatgcg aaaaatataa cgtcattccc
      721 gcgcaggcgg gaatctagtc tgtcggtgcg gaaacttatc ggataaaacg gtttctttag
      781 attttacgcc ct
//
LOCUS       DAWFJR010000191          791 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWFJR010000191 DAWFJR010000000
VERSION     DAWFJR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.191"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
BASE COUNT          204 a          191 c          201 g          195 t
ORIGIN      
        1 cccgtcattc ccacgaaagt gggaatctag aaatttaatg ttgcggcact agccaaaaaa
       61 accgaaaccg acaggtctag attcccgcct gcgcgggaat gacggatttt aggttggggt
      121 catttattgg aaaaagcaga aaccgctccg ccgtcattcc cacgaaagtg ggaatccagt
      181 tttttgagtt tcagtcattt tcaataaatt gccttagcat tgaatgtcta gattcccgcc
      241 tgcgcgggaa tgacgaatcc atccatacgg aaacctgcat cccgtcattc ccacgaacct
      301 acattccgtc attcccacga aagtgggaat ccagtttttt gagtttcagt cattcccgat
      361 aaattgcctt agtattgaat gtctagattc ccgcctgcgc gggaatgacg aacctatccg
      421 tacggaaacc tgcaccgcgt cattcccacg aaagtgggaa tccaggatgc agggttgggg
      481 aaaccgtgtt atccgataag tttccgcacc gacaggtcta gattcccgcc tgcgcgggaa
      541 tgacggattt taggttgggg tcatttattg ggaaaagcag aaaccgcttc gccgtcattc
      601 ccacgaaagt gggaatccag gatgcagggt tggggaaacc gtgttatccg ataagtttcc
      661 gcaccgacag gtctagattc ccgcctgcgg gggaatgacg gattttaggt tggggtcatt
      721 tattgggaaa agcagaaacc gctccgccgt cattcccacg aaagtgggaa tctagaaatt
      781 taatgttgcg g
//
LOCUS       DAWFJR010000192          772 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWFJR010000192 DAWFJR010000000
VERSION     DAWFJR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.192"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..477
                     /gene="mafB"
                     /locus_tag="WGR04_002101"
     CDS             <1..477
                     /gene="mafB"
                     /locus_tag="WGR04_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0795576.1"
                     /translation="SAAYNTRTTRKVTTETEGLNRIRQNQKNSNIHEKNYGRDNPNHI
                     NVLSGNSIQHILYGDEAGGGHLFPGKPGKTTFPQHWSASKITHEISDIVTSPKTQWYA
                     QTGTGGKYIAKGRPARWVSYETRDGIRIRTVYEPATGKVVTAFPDRTSNPKYNPVK"
     gene            492..722
                     /locus_tag="WGR04_002102"
     CDS             492..722
                     /locus_tag="WGR04_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ0795577.1"
                     /translation="MKTLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
BASE COUNT          306 a          128 c          141 g          197 t
ORIGIN      
        1 tctgctgcat acaatacaag aacaactaga aaagttacta cagaaacaga ggggttaaat
       61 agaatcagac agaaccagaa aaatagtaat atacatgaga aaaattatgg aagagataat
      121 cctaatcata ttaatgtttt atctggaaat tctatacaac atatactgta tggagatgaa
      181 gcaggaggtg ggcatctttt tcctggcaaa cctggtaaga caacattccc ccaacattgg
      241 tcagccagta aaataactca tgaaattagt gatatcgtta catccccaaa aacgcaatgg
      301 tatgcacaga ctggaacagg cggcaaatat attgctaaag gaagaccagc caggtgggta
      361 tcatatgaaa cgagagatgg aattcgtatc agaacagttt atgaacctgc aacaggaaaa
      421 gtggtaactg cattccccga tagaacctct aatcccaaat ataaccctgt aaaataatga
      481 gtatttgaaa gatgaaaaca ttagacgaac gaataaaaaa tttaggaaaa tctcttgaag
      541 atagaattga cgctaatcta attgatgccg cactagagta cattactttt tcagaacgtt
      601 tgctagcttt tgaaacgcta tgtgattata tagaagattt caatatccag cttacggaaa
      661 aagaatccca agaaatctct tttattaata aggaatttgg aattgaaagt acatcagatt
      721 aataaatatt aggtcggttt aaaaacccaa caatcaaaaa gtcgtctgaa ac
//
LOCUS       DAWFJR010000193          761 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWFJR010000193 DAWFJR010000000
VERSION     DAWFJR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.193"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(38..604)
                     /locus_tag="WGR04_002103"
     CDS             complement(38..604)
                     /locus_tag="WGR04_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ0795578.1"
                     /translation="MSLYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMREGLSGEAEDVRESKRES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVMVTVGLTAGRALGVLF
                     GRCAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT          212 a          256 c          155 g          138 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactatattt tccgacatca ttgaatcaaa cccaaatgcg
       61 acaagagcgt ccatgtgccg atggcaatca acaccaaacc tccggcaaat tccgcacacc
      121 tgccgaacaa tacgcccaaa gcccttcccg ctgtcagccc gaccgtaacc atcaccgtcg
      181 tcgccatacc gatgattgcg gcggcaaagg cgatgtttac ctccataaac gccaagccca
      241 ccccgactat catggaatca atactggttc caaaagcagt caaaaccgtc atccataggc
      301 tttcccgttt gctttcgcgc acatcttccg cctcgccgga cagcccttcg cgcatcattt
      361 tcagacccag cccgcccagc aggacgaaag ccgcccaatg gtcccattcg ctgataaacg
      421 gtttggcata aaaaccgcct acccagcccg ccagcggcgt gagtgcttca accgtgccga
      481 acaccaaagc cgttgctgca attttgcgcg ggggcatcct gaccgccgca ccctttgcca
      541 atgcgacggc aaacgcatcc atcgacatcc ccagcgcaat caagagcaaa gcgtaaagac
      601 tcatactgca cccgtcctca aaaagggcgg attatagcaa aacagcaaag cgcgaaaaac
      661 gccgcacgaa aacccacatc ccgtcattcc tgcaaaaaca aaaaatcaaa aacagaaatc
      721 ccgtcattcc cgcgcaggcg ggaatccaga gttgtcggtg c
//
LOCUS       DAWFJR010000194          757 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.194, whole genome shotgun sequence.
ACCESSION   DAWFJR010000194 DAWFJR010000000
VERSION     DAWFJR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.194"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(16..189)
                     /locus_tag="WGR04_002104"
     CDS             complement(16..189)
                     /locus_tag="WGR04_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795579.1"
                     /translation="MKKSLIALALSVLKPQMPEFEIKPASIGYLKQHPSMRLGKSGVA
                     AAKRAARKRKNRR"
     gene            complement(186..731)
                     /locus_tag="WGR04_002105"
     CDS             complement(186..731)
                     /locus_tag="WGR04_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0795580.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNDY
                     GLTVRTDGTGKGNMPLRDAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAAKV"
BASE COUNT          163 a          223 c          184 g          187 t
ORIGIN      
        1 caacctgtcc catgattaac gacgattctt gcgtttacgc gccgcgcgtt tcgcagccgc
       61 tacgcccgac ttgcccaggc gcatagacgg atgttgtttc aaatagccaa tactggcagg
      121 cttaatctca aattcaggca tctgcggttt caagacagac agagccaaag caatcaaaga
      181 ctttttcata ccttcgccgc tcccaattcc atcgcaatcg cgtccgcaat cgcgcggcag
      241 atgccccatt tggtcgtctt aaacaaggcc aaatcagtgt cgttgctgat aaaaaacggc
      301 tcaaacacaa tgccgccggc ttgtgcataa gccaggcgcg aatgctggcc cgcattgtcg
      361 ggtttaaagc cgtcttcgcc gcgcagtttc cagccggttt tcttggcaac agctttgctc
      421 aacacctgac accagcgttt gtttttaacg gtactcaagg cttcgatgcc tgtcgccgct
      481 ttgctgacgg cagcgttggt gtgaaactca atcgccacat ccgagccgcg aatcagcttg
      541 accgcatcgc gcaagggcat attgcctttg cccgtgccgt cggtgcgtac ggttaagccg
      601 taatcgttac gcaggattga agccacaatg ttgcgcatat cctgcgccaa atccgcctca
      661 cggtcgcttc cgttgaccgc gcccgggtcg gtgttgctgt gtccagcggt taaggttacg
      721 gttttgccca ttcatcatct ccaaaaaata ttgattg
//
LOCUS       DAWFJR010000195          754 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWFJR010000195 DAWFJR010000000
VERSION     DAWFJR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.195"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..399
                     /gene="mafB"
                     /locus_tag="WGR04_002106"
     CDS             <1..399
                     /gene="mafB"
                     /locus_tag="WGR04_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0795581.1"
                     /translation="SDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGAQGK
                     HISGHRNYIEGKSTLNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSDGKS
                     GLSTNFGTIHSGKNGVHIVPANPKTIKKVQ"
     gene            402..719
                     /locus_tag="WGR04_002107"
     CDS             402..719
                     /locus_tag="WGR04_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795582.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
BASE COUNT          270 a          113 c          153 g          218 t
ORIGIN      
        1 tcagactcct acaagcataa cactgcttca agattatctc agtctgtaga tggagaaatg
       61 tttcaaaccc gcaatgttga ttttaaagca aaatctattg ggactaaaat tcatgatgga
      121 gctcaaggga aacatatttc gggacataga aactacattg aaggtaagag tactttaaat
      181 caaaacatta atcctcaaga attgttgaac ggaatacatt caggtgctta tccagttatt
      241 tctaaaggag caagaggaaa tcctgttgtt gattttgggt atcctatagg cagcgatggg
      301 aaatcaggat taagtaccaa ttttggtacg attcattcag gtaaaaatgg agttcacatt
      361 gttccggcta accctaaaac cattaaaaag gtgcaatagt tatgaatata ttaccaagct
      421 ggctgcgagt cggtatgaat atagcaatgc tgggcatgat acactcagat atcaggttaa
      481 ttaccgtaga ttacgaggaa ggaagaaggt ttttaaaaat caaaaattat ttatcaagag
      541 aagccatcac agaagaccat gaagatatgg aatatttgat tacagagtta tggtctatgt
      601 gtggagaata ttttgatgaa gctgactttg aatgtattta ttctaatcat tcttctatgg
      661 agttaaacca aataaatggt gcagtattca ggagaaagga attaatttcg caagcgtagg
      721 ttaaaaaacc aacaatcaca atgtcttctg aaac
//
LOCUS       DAWFJR010000196          722 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWFJR010000196 DAWFJR010000000
VERSION     DAWFJR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.196"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            501..>722
                     /locus_tag="WGR04_002108"
     CDS             501..>722
                     /locus_tag="WGR04_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0795583.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLAEGQKSAVTEYYLNHGIWPGGNSSAGVA"
BASE COUNT          244 a          193 c          119 g          166 t
ORIGIN      
        1 atccgcacgg aaacctgccg cgatgattca actgccgttt gcacaggaac accacgcgcc
       61 gatttcaatc actttccaag aaaacggaaa tttttaaaaa ttaaatttaa aatttcccac
      121 ccaacccacc ctatgctacc gcgcaaattc aaaaatctca aattccgacc caatcaacac
      181 acccgatgcc ccatgccaat agagataccc cacgccaata aaaaagtgcc gaaaatcggc
      241 actaaaactg acaattttca acactgctga caaaaaacgg caacccccta attactcaaa
      301 ccacgccaaa cgaaagaaaa tgtaaaataa aaacaattac atagagataa acgcataaat
      361 tttcacctca aaatacaaac ttggtaccaa tcttgcttta taatgcacag gtatcgtaac
      421 aaaataccga tggtcaaata cattgcataa tgccgatggc gtaagcttga ggcatttcct
      481 ttccaattag gagtaatttt atgaacaccc ttcaaaaagg ttttaccctt atcgagctga
      541 tgattgtgat tgccatcgtc ggcattttag cggcagtcgc ccttcctgct tatcaagact
      601 acacagcccg cgcacaagtt tccgaagcca ttcttttggc tgaaggtcaa aaatcagccg
      661 tcacagagta ttacctgaat cacggcatat ggcccggcgg caacagttct gccggcgtgg
      721 ca
//
LOCUS       DAWFJR010000197          709 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWFJR010000197 DAWFJR010000000
VERSION     DAWFJR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.197"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..480
                     /locus_tag="WGR04_002109"
     CDS             <1..480
                     /locus_tag="WGR04_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0795584.1"
                     /translation="KDARWTKKNGLYKLGYKQHTRTDAEGYIEKLHITPANAHECKHL
                     PPLLEGLPKGTTVYADKGYDSAENRQHLEERQLLDGIMRKACRNRPLTETQTKHNRYL
                     SKIRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSASAAA"
BASE COUNT          190 a          166 c          197 g          156 t
ORIGIN      
        1 aaagatgccc gttggacaaa gaaaaacggc ctctacaaac tcggttacaa acaacatacc
       61 cgtaccgatg cggaaggcta tatcgagaaa ctgcacatta cccccgccaa tgcccatgag
      121 tgcaaacacc tgccgccgtt gttggaagga ctgcccaaag gtacgaccgt ctatgccgac
      181 aaaggctatg acagtgcgga aaaccggcaa catctggaag agcgtcagtt gttagacggc
      241 attatgcgca aagcctgccg caaccgtccg ctgacggaaa cgcaaaccaa acataaccga
      301 tatttgtcga agatccgtta tgtggtcgaa caaagcttcg gtacgctgca ccgcaaattc
      361 cgctatgcgc gggcagccta tttcggactg attaaagtga gtgcgcaaag ccatctgaag
      421 gcgatgtgtt tgaacctgtt gaaagcggcc aacaggttaa gtgcgtccgc tgccgcctaa
      481 aaagcagccc ggatgcctga ttatcgggta tccggggatt aagggggtgt ttgggtagaa
      541 ttaggaggtg ttatggggcg aaaacagccg aaaacctgtg tttgggtttc ggctgtcggg
      601 agggaaagga attttgcaaa ggtctcggtc tgaataatgg tggcgtcaac gacggcggcg
      661 gatgctttct ctatttttaa acctttttcg gtcagttgtc ggttaatca
//
LOCUS       DAWFJR010000198          670 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.198, whole genome shotgun sequence.
ACCESSION   DAWFJR010000198 DAWFJR010000000
VERSION     DAWFJR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.198"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(69..554)
                     /gene="fxsA"
                     /locus_tag="WGR04_002110"
     CDS             complement(69..554)
                     /gene="fxsA"
                     /locus_tag="WGR04_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ0795585.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRFRNAIEHKKD
                     E"
BASE COUNT          197 a          212 c          122 g          139 t
ORIGIN      
        1 tgttaatcca ctatattttc aaaccggaaa aatgccgtct gaaccttcag acggcattcc
       61 attcacattt attcgtcttt tttgtgttcg atggcgtttc ggaaacgatt gccgccgtaa
      121 ggctcttcaa ccgtatattc cccctcgata atatcgtcat cgcgggaaaa gccctctttt
      181 ctgcccgatt ggttcatgtt gaaaaaattt tccgcacctc ctgcctgcaa cactgcacct
      241 cccttaaacg gcagcagcag caataccgcc aacaccgagg atacgaatcc cggactcatc
      301 agacacaccg ccgccaccgt ataacggata ggccacaaca tctgataaac ggataccctc
      361 ccgccgcttc tcattgccgc gcccgccaat aaaagaccgg acagccccgt atgcctgagc
      421 atcagcacgc cggcggcaaa ggttgccgcc attagaaaca gcgtccaacc gccgcccaac
      481 caatcggcaa cccacacaat cgacataatc tccaaaaaca gcagcaccaa aaaaccgata
      541 ccgaaaaatc tcattgaccg tcatccttat atttaagtaa acagcaaacc gcccgaacag
      601 gactccaagc gagctgcctg taaatgatta caaaaccatg tgcttcaagc cgaaacaatg
      661 tgaaatcacg
//
LOCUS       DAWFJR010000199          644 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.199, whole genome shotgun sequence.
ACCESSION   DAWFJR010000199 DAWFJR010000000
VERSION     DAWFJR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.199"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
BASE COUNT          158 a          165 c          142 g          179 t
ORIGIN      
        1 agataatctt tgaatattgc cgctgcctta aggtctggat tcccgcctgc gcgggaatga
       61 cgaatccatc cgtacggaaa cctgcaccac gtcattccca cgaacccaca tcccgtcatt
      121 accacgaaag cgggaatcta ggacgcaggg ttaagaaaac ctacatcccg tcattcccgc
      181 aaaagcggga atccagtccg ttcagtttcg gtcatttccg ataaattcct gctgcttttc
      241 atttctggat tcccactttc gtgggaatga cggcggaagg gttttggttt tttccgataa
      301 attcttgagg cattgaaatt ctagattccc gcctgagcgg gaatgacgat tcataagttt
      361 cccgaaattc caacataacc gaaacctgac agtaaccgta gcaactgaaa cgtcattccc
      421 accacttttc gtcattcccg cgaaagaggg aatccagtcc gttcagtttc ggtcatttcc
      481 gataaattcc tgttgctttt catttctaga ttcccacttt cgtgggaatg acggcggaag
      541 ggttttggtt ttttccgata aattcttgag gcattgaaat tccagattcc cgcctacgcg
      601 ggaatgacgg ctgcagatgc ccgacggtct ttatagcgga ttaa
//
LOCUS       DAWFJR010000200          643 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.200, whole genome shotgun sequence.
ACCESSION   DAWFJR010000200 DAWFJR010000000
VERSION     DAWFJR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.200"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..287)
                     /locus_tag="WGR04_002111"
     CDS             complement(<1..287)
                     /locus_tag="WGR04_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ0795586.1"
                     /translation="MYALSEKYNDNGFKAYKVLGEGGGIHTEYNYKFDKSLNLNVLES
                     STGARSLEKVPVKVTASVSRAAVLSGVGKLARLGAKLSTRAVPYVGTALLA"
BASE COUNT          223 a          118 c           93 g          209 t
ORIGIN      
        1 tgggctaaaa gggctgttcc gacataagga actgcccttg tgcttaattt cgcgcctaag
       61 cgggcaagtt tgccgactcc tgacaagacg gcggcgcggg aaactgatgc agttacttta
      121 acggggactt tttcaagaga gcgtgcgcct gttgaacttt ctaatacatt caaattcaaa
      181 cttttatcaa atttgtaatt atattctgta tgaattcctc ctccctcacc taaaactttg
      241 tatgccttaa atccattatc gttatatttt tccgaaagtg catacatcaa tttcccattt
      301 ttaatttcta aatctgctga aaaagattta gcactcaaaa gaaacagaaa agaaattatt
      361 agaatccgaa acatcaattt ttccattgcc aataatgaaa aatgaatcat ccttgaattt
      421 aatttcaaag caagattcat taaaattaat tctattaaaa aaattatgac atttatccat
      481 tgagaaattt ttcaatagac cagtttcttt caaataacaa taaaacccat aagaaagagg
      541 cctatcagga taagattctg acaatttatc taaatcagaa tttgatatat agaaaaagtc
      601 acgttcattt ttattcatgg tttcaagcct caaatgtcta att
//
LOCUS       DAWFJR010000201          631 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.201, whole genome shotgun sequence.
ACCESSION   DAWFJR010000201 DAWFJR010000000
VERSION     DAWFJR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.201"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..305)
                     /locus_tag="WGR04_002112"
     CDS             complement(<1..305)
                     /locus_tag="WGR04_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795587.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGVGKLARLGAKFSTRAVPYVGTALLA"
     gene            complement(289..606)
                     /locus_tag="WGR04_002113"
                     /pseudo
     CDS             complement(289..606)
                     /locus_tag="WGR04_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
BASE COUNT          182 a          129 c          113 g          207 t
ORIGIN      
        1 tgggctaaaa gggctgttcc gacataggga accgcccttg tgctgaattt cgcgcctaag
       61 cgggcaagtt tgccgacccc cgccaatacg ccggcgcggg aaatgctggc ggttattttg
      121 gcgttgattc gggcttttgc gcccgtaggg atgtgtgtta aatctaccgt ttttattaaa
      181 tcagatgaat aagttttact atttttaggt acaaacttat gaattttcgc accttgtccg
      241 gtatcaactg aaacagtttc agatattttt actgcatttg cattcgcttc aaacgaatac
      301 atcatcaaaa ttgcaattat cgacaatttc gcaaaattca aatttgtata ttttatgacc
      361 atctttcaga gattctttaa ttaccatttc tcaattatca gaaaatgaga ttagccaaat
      421 atcatgttta attcttctat tccagaaaaa agagaaacaa tcaataacat tttcagactt
      481 attaatcttc gcaaattcaa caaattcaga ttgcgctata accgccatcg attgcccaaa
      541 atacttgcta gacggctgat atttataaag tgccaactgc gcctgcgtaa taaacggctt
      601 gttcatggtt ctgcctttca atgattgttt t
//
LOCUS       DAWFJR010000202          589 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.202, whole genome shotgun sequence.
ACCESSION   DAWFJR010000202 DAWFJR010000000
VERSION     DAWFJR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.202"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            11..>589
                     /locus_tag="WGR04_002114"
     CDS             11..>589
                     /locus_tag="WGR04_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0795588.1"
                     /translation="MYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKEQTSFNNP
                     EPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDMPGQDAYSINLIEISVYY
                     KKTDQGWVLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERLLTYGVKK
                     MPSAYPEYEAYEDKRHIPENPYFHEFYYIKKGE"
BASE COUNT          197 a          114 c          115 g          163 t
ORIGIN      
        1 tgctactacc atttatcaac tttttatttt gtttattggg agtgttttta ctatgacctc
       61 atgtgaacct gtgaatgaaa agacagatca aaaagcagta agtgcgcaac aggctaaaga
      121 acaaaccagt ttcaacaatc ccgagccaat gacaggattt gaacatacgg ttacatttga
      181 ttttcagggc accaaaatgg ttatccctta tggctatctt gcacggtata cgcaagacaa
      241 tgccacaaaa tggctttccg acatgcccgg gcaggatgct tactccatta atttgataga
      301 gattagcgtc tattacaaaa aaaccgacca aggctgggtt cttgagccat acaaccagca
      361 aaacaaagca cactttatcc aatttctacg cgacggtttg gatagcgtgg acgatattgt
      421 tatccgaaaa gatgcgtgta gtttaagcac gactatggga gaaagattgc ttacttacgg
      481 ggttaaaaaa atgccatctg cctatcctga atacgaggct tatgaagata aaagacatat
      541 tcctgaaaat ccatattttc atgaatttta ctatattaaa aaaggagaa
//
LOCUS       DAWFJR010000203          572 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.203, whole genome shotgun sequence.
ACCESSION   DAWFJR010000203 DAWFJR010000000
VERSION     DAWFJR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.203"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..>572
                     /locus_tag="WGR04_002115"
     CDS             <1..>572
                     /locus_tag="WGR04_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795589.1"
                     /translation="GIEKGVKAIKELSEKMKNAASDLADGSAEKAKQVVEDLAQAAKE
                     AYENAKSTAEKAAQAAREFFKGLPSFKDLAEKFRDLFPNPEGWIDDGHQCLAPWVKET
                     KKRNGKYHVYDPLALDLDGDGIETVATKGFAGSLFDHTNNGIRTATGWVSADDGLLVR
                     DLNGNGIIDNGAELFGDNTKLADGSFAKHG"
BASE COUNT          176 a          137 c          136 g          123 t
ORIGIN      
        1 ggaatagaaa aaggtgtcaa agccattaaa gaattgtctg aaaaaatgaa aaatgctgct
       61 tccgatttgg ctgacggttc agcagagaaa gctaaacaag tagtggaaga tttggctcaa
      121 gccgccaaag aagcatacga aaatgccaaa tccacagccg agaaggctgc tcaagcagct
      181 cgagaatttt ttaagggctt gcccagtttt aaagatctgg ccgaaaaatt tagagatctg
      241 ttcccaaatc cggaaggctg gatcgatgat ggtcaccaat gtttagctcc ttgggttaaa
      301 gaaactaaaa aacgcaatgg caaatatcat gtctacgacc cccttgccct agacctagat
      361 ggcgacggta tagaaaccgt tgccaccaaa ggctttgcag gcagcttatt tgatcacacc
      421 aacaacggca tccgcaccgc caccggttgg gtttctgccg atgacggttt actcgtccgc
      481 gatttgaacg gcaacggcat catcgacaac ggcgcggaac tcttcggcga caacaccaaa
      541 ctggcagacg gttcttttgc caaacacggc ta
//
LOCUS       DAWFJR010000204          571 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.204, whole genome shotgun sequence.
ACCESSION   DAWFJR010000204 DAWFJR010000000
VERSION     DAWFJR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.204"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..248
                     /locus_tag="WGR04_002116"
     CDS             <1..248
                     /locus_tag="WGR04_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0795590.1"
                     /translation="DGIMRKACRNRPLSEAQTKRNRYLSKTRYVVEQSFGTLHRKFRY
                     ARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
BASE COUNT          153 a          133 c          153 g          132 t
ORIGIN      
        1 tggacggcat tatgcgcaaa gcctgccgca accgtccgct gtcggaagcg caaaccaaac
       61 gcaaccggta tttgtcgaag acccgttatg tggtcgaaca aagcttcggt acgctgcacc
      121 gtaaattccg ctatgcgcgg gcagcctatt tcgggctgat taaagtgagt gcgcaaagcc
      181 acctgaaggc gatgtgtttg aacctgttga aagcggccaa caggttaagt gcgcccgctg
      241 ccgcctaaaa ggcagcccgg atgcctgatt atcgggtatc cgggcaggat taaggggata
      301 tttgggtaga attaggcggt atttggggcg aaaatagacg aaaacctgtg tttgggtttc
      361 ggctgtcggg agggaaagga attttgcaaa ggtctcatcc tgttattttc acaaaaacag
      421 aaaaccaaaa acagcaacct gaaattcgtc attcccacga aagtgggaat ccagtttctt
      481 gagtttcagt cattcccgat aaattgcctt agcattgaat gtctagattc ccgcctgcgc
      541 gggaatgacg aatccatcca tacggaaacc t
//
LOCUS       DAWFJR010000205          566 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.205, whole genome shotgun sequence.
ACCESSION   DAWFJR010000205 DAWFJR010000000
VERSION     DAWFJR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.205"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
BASE COUNT          144 a          113 c          142 g          167 t
ORIGIN      
        1 gggcatctgc agccgtcatt cccgcgcagg cgggaatcta gaccttaagg cagcggcaat
       61 attcaaagat tatctgaaag tccgagattc tagattccca ctttcgtggg aatgacggtt
      121 cagttgctac ggttattgtc aggtttcggt tatgttggaa tttcgagaaa cttatgaatt
      181 gtcattcccg cgcaggcggg aatctagaat ttcaatgcct caagaattta tcggaaaaaa
      241 acaaaaccct tccgccgtca ttcccacgaa agtgggaatc tagaaatgaa aagcaacagg
      301 aatttatcgg aaatgaccga aactgaacgg actggattcc cgcttttgcg ggaatgacgg
      361 ttcagttgct acggttactg tcaggtttcg gttatgttgg gatttcggga aacttatgaa
      421 tcgtcattcc cgcgcaggcg ggaatctaga ccgttcggta cggaaactta tcggataaaa
      481 cggtttcttc agattttacg ttctagattc ccactttcgt gggaatgacg ggattttagg
      541 tttctgattt tggttttctg tttttg
//
LOCUS       DAWFJR010000206          558 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.206, whole genome shotgun sequence.
ACCESSION   DAWFJR010000206 DAWFJR010000000
VERSION     DAWFJR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.206"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            133..369
                     /locus_tag="WGR04_002117"
     CDS             133..369
                     /locus_tag="WGR04_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795591.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSFVSVKRQEVENESRETAGE"
     gene            341..>558
                     /locus_tag="WGR04_002118"
     CDS             341..>558
                     /locus_tag="WGR04_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795592.1"
                     /translation="MNLVKLLANNWQPIAIIALIGTGLAVSHHQGYYKSAFAKQQAVI
                     EKMEKDKAQALLLSAQNYARELEQARAEA"
BASE COUNT          137 a          123 c          178 g          120 t
ORIGIN      
        1 gctgtggtcg gccaaaagcc gtgaggcaag tgaacaggca gacttggcgg cgtttgaatt
       61 tgcggagggc gaactggcca attatcagga aatgctgaaa cggcacctgc aaaccaaaag
      121 tgtggaatag caatgcgtat tttggatatt tttaaaaacc cggcgacagg caatgtgtcg
      181 cactcgaaac tgtgggcaaa cgttgcctgc gcggcgggga cgtttaagtt tgtgatgctg
      241 cccgatccgt cggcggagat ttgggcggtg tatttgggca ttgtcggcgg ctatgcggtg
      301 gcgcgctcgt ttgtcagcgt caaacgtcag gaggtcgaga atgaatctcg tgaaactgct
      361 ggcgaataac tggcaaccaa ttgccatcat cgcacttatc ggcacgggtt tggcggtgtc
      421 gcaccatcaa ggctattata agtcggcgtt tgcgaagcag caggcggtca ttgagaaaat
      481 ggaaaaagac aaagcgcaag ccctgctgtt gtcggctcaa aactatgcac gcgagctgga
      541 acaggcgcgt gcggaagc
//
LOCUS       DAWFJR010000207          531 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.207, whole genome shotgun sequence.
ACCESSION   DAWFJR010000207 DAWFJR010000000
VERSION     DAWFJR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.207"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
BASE COUNT          138 a          104 c          138 g          151 t
ORIGIN      
        1 gaagtcttct actgatttgc acgcgaaaat gccgtccgaa aggttttcgg acggcatttt
       61 ttgcgttttt cgggaggggc gggttcgtaa aaggcgggct atagggtagg cttcatctcg
      121 ccaatctcac tgaatccatc aatttccaca attcaattaa ataccgtcaa accgatgccg
      181 tcattcccgc gcaggcggga atctagacat tcaatgctaa ggcaatttat cgggaatgac
      241 tgaaactcaa gaaactggat tcccactttc gtgggaatga tgggatgcag gttcgtggga
      301 atgacgtggt gcaggtttcc gtgcggatgg attcgtcatt cccgcgcagg cgggaatcca
      361 gacattcaat gctaaggcaa tttatcggga atgactgaaa ctcaaaaaac tggattccca
      421 ctttcgtggg aatgacggga ttttaggttt ctgattttgg ttttctgttt ttgtaggaat
      481 gatgaaattt tgagttttag gaatttaccg gaaaaaacag aaaccgttct g
//
LOCUS       DAWFJR010000208          508 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.208, whole genome shotgun sequence.
ACCESSION   DAWFJR010000208 DAWFJR010000000
VERSION     DAWFJR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.208"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            317..>508
                     /locus_tag="WGR04_002119"
     CDS             317..>508
                     /locus_tag="WGR04_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0795593.1"
                     /translation="MTMQVFLTIPGYDVEAEIEKFVWMDAVIWQMPSWWMHEPWTVKK
                     YIDEVLTAGHGKLYQSDGRH"
BASE COUNT          152 a          125 c          123 g          108 t
ORIGIN      
        1 tgcgcgggaa tgacaactca ttagttacct aaaacttaaa aaacggaaac ctttacgccg
       61 tcattcccac gaaagtggga atccgggaac ttaacgttac agtgatttat cggaaacggc
      121 tgaaaccgaa tgaattggat tcccgcctgc gcgggaatga cgaatccatc cgtacggaaa
      181 cctgcaccac gtcattccta cgaacctaca ttccgtcatt cccacgaaag tgggaatcca
      241 ggacgaaaaa tctccagaaa ccgttttacc cgataagttt ccgcaccgac aggtctggat
      301 tcccgcctgc acgggaatga cgatgcaggt atttctgacg attcccggct atgatgttga
      361 ggcagaaatc gaaaaattcg tttggatgga tgctgtgatt tggcagatgc cgagctggtg
      421 gatgcacgag ccttggacag tgaaaaaata catagacgaa gtattaaccg ctggacacgg
      481 caaactctac caaagcgacg gcagacac
//
LOCUS       DAWFJR010000209          497 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.209, whole genome shotgun sequence.
ACCESSION   DAWFJR010000209 DAWFJR010000000
VERSION     DAWFJR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.209"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
BASE COUNT          124 a          121 c          102 g          150 t
ORIGIN      
        1 taaaaaattt atgctattaa atcagtaatt tctgatgaat tttgaaaact taatcccgtc
       61 attcccgcgc aagcgggaat ccggctcgtt cggtttcgct tgttttaagt ttcgggtaac
      121 ttccacttcg tcattcccgc gcaggcggga atccggttca ttgagtttca gctatttaga
      181 ataaattttg aaactctaat cgcgtcattc ccacgaaagt gggaatccag gacgcaaaat
      241 ctcaagaaac cgttttacct gataagtttc cgcactgaca gacctagatt cccgcctgcg
      301 cgggaatgac gaatccatcc atacggaaac ctgcatcccg tcattcccac gaaagtggga
      361 atccggttcg ttcggtttcg cttgttttaa gtttcgggta acttccactt cgtcattccc
      421 acgaaagtgg gaatccagct ttttgagttt cagtcattcc cgataaattg ccttagcatt
      481 gaatgtctgg attcccg
//
LOCUS       DAWFJR010000210          464 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.210, whole genome shotgun sequence.
ACCESSION   DAWFJR010000210 DAWFJR010000000
VERSION     DAWFJR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.210"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            4..330
                     /locus_tag="WGR04_002120"
     CDS             4..330
                     /locus_tag="WGR04_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0795594.1"
                     /translation="MPDHLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRF
                     WEHTIRDEADFACHFDYLHFNPVKHGYVGQISDWRFSTFHRYVKQGIYPHNWGGGNAD
                     FSIEYD"
     gene            367..464
                     /locus_tag="WGR04_002121"
                     /pseudo
     CDS             367..464
                     /locus_tag="WGR04_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          123 a          109 c          113 g          119 t
ORIGIN      
        1 ttgatgcccg accatctgca taccatatgg cggctgccgg acaatgattc tgcttattcg
       61 gaacgctggc ggcaaatcaa gcggcacagc caatatttaa tcggcggcaa tctcaggctt
      121 tggcaaaaac gcttttggga acatactatc cgcgatgagg ccgattttgc ctgccatttt
      181 gattatctgc atttcaatcc ggtcaaacat ggctatgtag gacaaatttc cgattggcgg
      241 ttttctacgt ttcaccgtta tgtcaaacag ggtatttatc cgcataattg gggtggcggc
      301 aatgcggact tttctattga atacgattaa agtaatgtcg gattcaagaa tccgacctac
      361 ggaaaactga aagagcatcg gctgcaggac ggcattatgc gcaaagcctg ccgcaaccgt
      421 ccgctggcgg aagtgcaaac caaacgtaac cgatatttgt cgaa
//
LOCUS       DAWFJR010000211          464 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.211, whole genome shotgun sequence.
ACCESSION   DAWFJR010000211 DAWFJR010000000
VERSION     DAWFJR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.211"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            4..330
                     /locus_tag="WGR04_002122"
     CDS             4..330
                     /locus_tag="WGR04_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0795595.1"
                     /translation="MPDHLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRF
                     WEYTIRDEADFACHFDYLHFNPVKHGYVGQISDWGFSTFHRYVKQGIYPHNWGGGNAD
                     FSIGYD"
     gene            367..464
                     /locus_tag="WGR04_002123"
                     /pseudo
     CDS             367..464
                     /locus_tag="WGR04_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a          107 c          115 g          120 t
ORIGIN      
        1 ttgatgcccg accatctgca taccatatgg cggctgccgg acaatgattc tgcttattcg
       61 gaacgctggc ggcaaatcaa gcggcacagc caatatttaa tcggcggcaa tctcaggctt
      121 tggcaaaaac gcttttggga atatactatc cgcgatgagg ccgattttgc ctgccatttt
      181 gattatctgc atttcaatcc ggtcaaacat ggctatgtag gacaaatttc cgattggggg
      241 ttttctacgt ttcaccgtta tgtcaaacag ggtatttatc cgcataattg gggtggcggc
      301 aatgcggact tttctattgg atacgattga agtaatgtcg gattcgagaa tccgacctac
      361 ggaaaactga aagagcatcg gctgcaggac ggcattatgc gcaaagcctg ccgcaaccgt
      421 ccgctgacgg aaaatcaaac caagcgtaac cgatatttgt cgaa
//
LOCUS       DAWFJR010000212          461 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.212, whole genome shotgun sequence.
ACCESSION   DAWFJR010000212 DAWFJR010000000
VERSION     DAWFJR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.212"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..>461
                     /locus_tag="WGR04_002124"
     CDS             <1..>461
                     /locus_tag="WGR04_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0795596.1"
                     /translation="PAAKAIRRAGIAVIIANPRQTHQFAQSQPLTKTDAKDAKMPAFF
                     TQMMTQKEDWQTMPYQPPTEAEEVLEALVNRRNQSADMRTAEKNRLHQVHETQVGSVK
                     QLIAHFDRLIDELDKQIDNHTHTHFDGKAQVAEQIKGIGSITTATLMAMLP"
BASE COUNT          137 a          139 c          111 g           74 t
ORIGIN      
        1 cccgccgcca aagccatccg ccgagcaggc atagccgtga ttatcgccaa cccgcgtcag
       61 acgcatcagt ttgcccaatc gcagccgctg accaaaaccg acgccaaaga tgccaaaatg
      121 cccgccttct tcacacagat gatgacgcag aaagaagatt ggcaaaccat gccctaccaa
      181 ccgcccaccg aagcggaaga agtgttggaa gccttggtta accgccgcaa ccaatcggcg
      241 gatatgcgga ctgccgagaa aaaccgtctg catcaggttc atgaaacgca agtcggaagc
      301 gtcaaacaac tgattgccca ttttgaccgg ctgattgacg aattggacaa acaaatcgac
      361 aaccacaccc acacgcattt tgacggcaaa gcccaagtgg cagagcaaat caaaggcatc
      421 ggttcgataa cgacggctac gctgatggcg atgttgcctg a
//
LOCUS       DAWFJR010000213          453 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.213, whole genome shotgun sequence.
ACCESSION   DAWFJR010000213 DAWFJR010000000
VERSION     DAWFJR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.213"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(305..>453)
                     /locus_tag="WGR04_002125"
     CDS             complement(305..>453)
                     /locus_tag="WGR04_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0795597.1"
                     /translation="GSVKWFCGQPVTRTGTGTNSDDVTADSDKQIDTKHLPSTAPTRK
                     STPN"
BASE COUNT          111 a           90 c          112 g          140 t
ORIGIN      
        1 aaagtttcaa aatttattct aaataactga aactcaacga actagattcc cgcttttgcg
       61 ggaatgacgg gattaaagtt tcaaaattta ttctaaataa ctgaaactca acgaactaga
      121 ttcccacttt cgtgggaatg acgaatttta ggtttctgtt tttggttttc tgtttttgag
      181 ggaatgatga aattttaagt tttaggaatt tatcagaaaa aacagaaacc gctccgccgt
      241 cattcccgcg caggcgggaa tccagcctcg tcggtacgga aacttatcgg gtaaaaaggt
      301 ttctctagtt tggtgtcgat tttcttgtcg gtgctgttga cggcaggtgc ttggtgtcga
      361 tctgcttgtc gctgtcggcg gtaacgtcgt cggagttggt gccggtgccg gtgcgcgtaa
      421 ccggctgtcc gcagaaccat tttaccgaac cgt
//
LOCUS       DAWFJR010000214          434 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.214, whole genome shotgun sequence.
ACCESSION   DAWFJR010000214 DAWFJR010000000
VERSION     DAWFJR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.214"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
BASE COUNT          120 a           93 c          118 g          103 t
ORIGIN      
        1 cggatggatt cgtcattccc gcgcaggcgg gaatccagac attcaatgct aaggcaattt
       61 atcggaaatg actgaaactc aaaaaactgg attcccactt tcgtgggaat gacggaatgt
      121 aggttcgtgg gaatgacggg atttgagatt gcggcattta tcggaaaaac agaaaccgct
      181 ccgccgtcat tcccgcgcag gcgggaatct agaccttaga acaacagtaa tattcaaaga
      241 ttataaaaga cccgtcattc ccgcgtaggc gggaatctag accttagaac aacagtaata
      301 ttcaaaggtt agctgaagct ttagagattc tggattccca ctttcgtggg aatgacggga
      361 ttttaggttc gcgggaatga cgcggtgcag gtttccgtgc ggatggattc gtcattcccg
      421 cgcaggcggg aatc
//
LOCUS       DAWFJR010000215          432 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.215, whole genome shotgun sequence.
ACCESSION   DAWFJR010000215 DAWFJR010000000
VERSION     DAWFJR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.215"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
BASE COUNT          124 a          115 c          110 g           83 t
ORIGIN      
        1 ccaacgctgc aagatttatc agaaacaact gaaaccgaac ggactggatt cccgcctgcg
       61 cgggaatgac gggattttag taaccgtagc aaccgcctgc gcgacggcta aggggcttca
      121 gcaaccgtag caactgcctg tgtgggaatg acggacaatg ggcttcagac ggcatcagac
      181 ggcatctctt gcctgccgct aaaacagttt gccgcacaac tgttcaaacg cgtccgatat
      241 atttcaacac acaggacgac acataaagca cctccctatg tgtcgtcctg atttggaagg
      301 ggttacaccc cctcccaaat aaagtctgat cctgccgccc cgaaggacag atgtccgagt
      361 ggcggggttt caaccgaaaa ggaaatacga tgaagtggta caattagcgg caatgcggac
      421 agacaaatta aa
//
LOCUS       DAWFJR010000216          428 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.216, whole genome shotgun sequence.
ACCESSION   DAWFJR010000216 DAWFJR010000000
VERSION     DAWFJR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.216"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..393
                     /locus_tag="WGR04_002126"
     CDS             <1..393
                     /locus_tag="WGR04_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0795598.1"
                     /translation="ILLAEGQKSAVTEYYLNHGIWPGDNSDADVASSSKIKGKYVKEV
                     TVANGVVTAEMKSDGVNNEIKGKKLSLWAKRQDGSVKWFCGQPVECADKAGTNNDEVK
                     AATGTTGNKKIDTKHLPSTAPTRKSTPN"
BASE COUNT          143 a          118 c           97 g           70 t
ORIGIN      
        1 attcttttgg ctgaaggtca aaaatcagcc gtcacagagt attacctgaa tcacggcata
       61 tggcccggcg acaacagtga tgccgacgtg gcatcctcct ctaaaatcaa aggaaaatat
      121 gttaaggaag ttacagtcgc aaacggcgtc gttaccgccg aaatgaaatc agacggcgta
      181 aacaacgaaa tcaaaggcaa aaaactctcc ctgtgggcca agcgtcaaga cggttcggta
      241 aaatggttct gcggacagcc ggttgagtgc gccgacaaag ccggcaccaa caacgacgaa
      301 gtcaaagccg ccaccggcac caccggcaac aagaaaatcg acaccaagca cctgccgtca
      361 acagcaccga caagaaaatc gacaccaaac tagagaaacc tttttacccg ataagtttcc
      421 gtaccgac
//
LOCUS       DAWFJR010000217          402 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.217, whole genome shotgun sequence.
ACCESSION   DAWFJR010000217 DAWFJR010000000
VERSION     DAWFJR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.217"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..>402
                     /locus_tag="WGR04_002127"
     CDS             <1..>402
                     /locus_tag="WGR04_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795599.1"
                     /translation="LAKNIYQNLLFQTRLQPYLNQISFKMENDTFTLDFSGLVQAFNH
                     VKETNPQKAFVDLAEMLAYGELRSWYEGRRLMADYVEEAKKAGKFEDYQKVLGQETVA
                     LLAKTSGTQADDILQNVGFGHNKNVSLYGNDG"
BASE COUNT          129 a           69 c           89 g          115 t
ORIGIN      
        1 tctcgccaaa aacatctacc aaaacctgtt gttccaaacc cgtttgcagc catatttgaa
       61 tcaaatcagt ttcaaaatgg aaaatgatac gttcactttg gattttagtg gtcttgttca
      121 agcatttaac catgtcaaag aaactaatcc gcaaaaagct tttgtggatt tggccgagat
      181 gcttgcatat ggcgaacttc gttcttggta tgaaggccga agactaatgg ccgattatgt
      241 ggaggaggca aaaaaagcag gtaaatttga agattaccag aaagtgttgg gtcaggagac
      301 cgttgcatta ttagctaaaa catcgggtac gcaagcagat gatatcctgc aaaatgtagg
      361 ctttggtcat aataaaaatg tttctttata tggtaatgac gg
//
LOCUS       DAWFJR010000218          386 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.218, whole genome shotgun sequence.
ACCESSION   DAWFJR010000218 DAWFJR010000000
VERSION     DAWFJR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.218"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
BASE COUNT          148 a           32 c           76 g          130 t
ORIGIN      
        1 ctagattccc actttcgtgg gaatgacggg atgtgggttt ttgtgcggat ttgaaccagt
       61 aagggtggtg tgggattggt ggtttgctta ggatcttttg gattgtattt tgtatataca
      121 tttacttgtt gataaaagat aaaataaaat tagaaactaa aagtgagaaa aaattaataa
      181 taatagggat gtataaatgt aaagactccg tttcatagct aaggttatct gaatatatgg
      241 aaaaaaagta aaagtccata aaactaaaat atatagataa tgctaatgat aatagaatta
      301 tcactttcct agagtaagga taatattttt ttatcgtaaa aaaatataaa ataggataaa
      361 taattgccat atagatagat tttgta
//
LOCUS       DAWFJR010000219          382 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.219, whole genome shotgun sequence.
ACCESSION   DAWFJR010000219 DAWFJR010000000
VERSION     DAWFJR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.219"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
BASE COUNT           92 a          102 c          115 g           73 t
ORIGIN      
        1 gtcgggcatc tgcagcggtt tgctaaaaaa cgctttaccg tgatcagtgt gcaaagttaa
       61 aatggggagg taagcttttc aaccagcaat tcggcgggcg cgggatcggg cggtttgccg
      121 aatcccgccg tttgcggcgc gcctgccgcc gacggtatcc cgcgaagcaa gatttaaggg
      181 ataaaatatg ttccaacacg cagggcggca cataaggcgc cgccctgatt cggaagggct
      241 tgcacccctc ccggacaaag cctgatcctg ccgccccgaa ggacggatgc ccgaagggcg
      301 gggggtttga ccgaaaagga aatacgatga ataaaacttt aaaaaggcgg gttttccgcc
      361 ataccgcgct ttatgccgcc at
//
LOCUS       DAWFJR010000220          377 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.220, whole genome shotgun sequence.
ACCESSION   DAWFJR010000220 DAWFJR010000000
VERSION     DAWFJR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.220"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            160..377
                     /locus_tag="WGR04_002128"
                     /pseudo
     CDS             160..377
                     /locus_tag="WGR04_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          112 a          100 c           84 g           81 t
ORIGIN      
        1 atagtggatt aacaaaaacc agtacggcgt tgcctcgcct tagctcaaag agaacgattc
       61 tctaaggtgc tgaagcacca agtgaaagtg aatcggttcc gtactatttg tactgtctgc
      121 ggcttcgtcg ccttgtcctg atttttgtta atccactata ctttatgccg ctaccgcaac
      181 tggctggcgc aagacaatac cctgtccgaa ctgttggaac tgattaaccg ccaactgacc
      241 gaaaaaggct taaaaataga gaaagcatcc gccgccgccg ttgacgccac cattattcaa
      301 accgccggca gcaaacagcg tcaggccata gaagttgacg aagaaggaca aatcagcggc
      361 caaaccacac cgagtaa
//
LOCUS       DAWFJR010000221          335 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.221, whole genome shotgun sequence.
ACCESSION   DAWFJR010000221 DAWFJR010000000
VERSION     DAWFJR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.221"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
BASE COUNT          101 a           83 c           68 g           83 t
ORIGIN      
        1 ttcgcgggaa tgacggcggc gggatttctg tttttccgac aaattcctaa aacttaaaat
       61 tccgttattc ccgcgagaac agaaaaccaa aaacagaaac ctaaaattcg tcattcccgc
      121 gcaggcggga atccagtccg ttgagtttca gtcatttccg ataaatcacc gcaacgttaa
      181 atttctagat tcccgctttc gcgggaatga cggcgggggg atttctgttt ttccgacaaa
      241 ttcctaaaac ttaaaattcc gttattcccg cgagaacaga aaaccaaaaa tagaaaccta
      301 aaatctcgtc attcccgcgc aggcgggaat ccagt
//
LOCUS       DAWFJR010000222          302 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.222, whole genome shotgun sequence.
ACCESSION   DAWFJR010000222 DAWFJR010000000
VERSION     DAWFJR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.222"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..>302
                     /locus_tag="WGR04_002129"
     CDS             <1..>302
                     /locus_tag="WGR04_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795600.1"
                     /translation="AEQAKAANLAGIGRLRDLREAAALSGDLANMLKAYSAAETKEAQ
                     LALLDNLIHKWAETDSNWGKKSPMRLSTDWTQTANEGIALTPSQVAQLKKNALVSLS"
BASE COUNT           88 a           76 c           72 g           66 t
ORIGIN      
        1 gccgaacagg caaaagccgc caatcttgcg ggcatcggcc gtctgcgcga tttgcgcgaa
       61 gctgccgcat tgtccggcga tttggccaat atgctgaaag cttattctgc cgccgaaact
      121 aaagaagcac agttggcatt gttagataat ttgattcaca aatgggcgga aaccgattcg
      181 aactggggca aaaaatcgcc aatgcgactt tcaaccgatt ggacgcaaac ggctaatgaa
      241 ggtattgcac tgacaccatc ccaagtagca caactaaaaa agaacgcttt agtttccctt
      301 tc
//
LOCUS       DAWFJR010000223          295 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.223, whole genome shotgun sequence.
ACCESSION   DAWFJR010000223 DAWFJR010000000
VERSION     DAWFJR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.223"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..>295)
                     /locus_tag="WGR04_002130"
     CDS             complement(<1..>295)
                     /locus_tag="WGR04_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEZ0795601.1"
                     /translation="PFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLGMAIG
                     NTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML"
BASE COUNT          106 a           57 c           63 g           69 t
ORIGIN      
        1 agcatgattg catcggtaac gacagcattt aatcctacat cggcaatatc gccaaaagtg
       61 aggcggaaac catacagcac aatgccagtg cgaagaagcg cgcctttggc aaacaaaacg
      121 cctttttcca cttgtgttga aaattgcgga taaatggtat tgccgattgc cattcccagc
      181 aagatagcaa taattaaagc actgatatga taatgatggg aaaaatcagt gtttcctaaa
      241 tagttagcaa gtatagcgat aatcgcgata aatataagtc cgaaataaaa gggac
//
LOCUS       DAWFJR010000224          290 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.224, whole genome shotgun sequence.
ACCESSION   DAWFJR010000224 DAWFJR010000000
VERSION     DAWFJR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.224"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
BASE COUNT          102 a           58 c           60 g           70 t
ORIGIN      
        1 tttttgttaa tccactataa tttattgaag atggctgaaa ttcaaagtta cagaaattta
       61 taaacaaaaa acagcaaccc gccgccgtca ttcccgcgaa agcgggaatc cagtcccttg
      121 accttaagat aatctttaat tcgtcattcc cgcaaaagcg agaatccgga agcgaaaagt
      181 ggtgggaatg tattagaaat gactgaaact caaaaaaccg gattcccgcc tgcgcgggaa
      241 tgacgattga tacgtttcga tatagtggat taacaaaaat caggacaagg
//
LOCUS       DAWFJR010000225          285 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.225, whole genome shotgun sequence.
ACCESSION   DAWFJR010000225 DAWFJR010000000
VERSION     DAWFJR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.225"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..226
                     /locus_tag="WGR04_002131"
     CDS             <1..226
                     /locus_tag="WGR04_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ0795602.1"
                     /translation="GWYDFRCQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAKSE
                     QRLSVLSEWVGQLETEYSELNLNRYSVASP"
BASE COUNT           71 a           73 c           73 g           68 t
ORIGIN      
        1 cggctggtac gatttccgct gccaaaacgt cgtgtacgcg cccgaccgca gcccggaaat
       61 cagccgtatg ctttcttcga ttttggcagg ctacgcgtgg gataccgaaa acccgttcgt
      121 ggcgaaatcc gaacaacgcc tgtcggtctt atcagaatgg gtcggtcagt tggaaaccga
      181 atatagtgaa ttaaatttaa accggtacag cgttgcctcg ccttagctca aagagaacga
      241 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatt
//
LOCUS       DAWFJR010000226          270 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.226, whole genome shotgun sequence.
ACCESSION   DAWFJR010000226 DAWFJR010000000
VERSION     DAWFJR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.226"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..>270
                     /locus_tag="WGR04_002132"
     CDS             <1..>270
                     /locus_tag="WGR04_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0795603.1"
                     /translation="VDKNSDTMHFKGFKAADVHFIRSGSDLVLSASEQDNVRISGFFY
                     GENHRVDTYVFDDAAISNPDFAKYINAGNNLVQSMSVFGSNTAAT"
BASE COUNT           75 a           48 c           57 g           90 t
ORIGIN      
        1 atgtggataa aaactctgac actatgcact ttaaaggatt taaagcagca gatgttcatt
       61 ttatccgttc cggaagtgat ttggtgctta gcgcttctga acaagacaac gtacgtattt
      121 ccggattctt ctatggtgaa aaccatcgtg tagatacata tgtctttgat gatgcagcta
      181 tcagtaatcc agattttgcc aagtatatta atgctggcaa taatttggta cagtctatgt
      241 ctgtgttcgg ttctaatact gctgcgacag
//
LOCUS       DAWFJR010000227          261 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.227, whole genome shotgun sequence.
ACCESSION   DAWFJR010000227 DAWFJR010000000
VERSION     DAWFJR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.227"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..>261
                     /locus_tag="WGR04_002133"
     CDS             <1..>261
                     /locus_tag="WGR04_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0795604.1"
                     /translation="NNSDAGVASSASDIKGKYVQSVIVANGVVTAEMKSTGVNKEIKG
                     KELSLWAKRQNGSVKWFCGQPVTRTGTGTNSDDVTADSDKKI"
BASE COUNT           80 a           76 c           65 g           40 t
ORIGIN      
        1 caacaacagt gatgccggcg tggcatcctc cgcctccgac atcaaaggca aatatgttca
       61 aagcgttata gtcgcaaacg gcgtcgttac cgccgaaatg aaatcaaccg gcgtaaacaa
      121 agaaatcaaa ggcaaagaac tctccctgtg ggccaagcgt caaaacggtt cggtaaaatg
      181 gttctgcgga cagccggtta cgcgcaccgg caccggcacc aactccgacg acgttaccgc
      241 cgacagcgac aagaagatcg a
//
LOCUS       DAWFJR010000228          249 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.228, whole genome shotgun sequence.
ACCESSION   DAWFJR010000228 DAWFJR010000000
VERSION     DAWFJR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.228"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            <1..>249
                     /locus_tag="WGR04_002134"
     CDS             <1..>249
                     /locus_tag="WGR04_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HEZ0795605.1"
                     /translation="IGDILYGTRYAIDKAAMRNIAPLPAEGKFAAIGGLGSVAGFEKN
                     TREAVDRWIQENPNAAETVEAVFNVAAAAKVAKLAKAA"
BASE COUNT           61 a           77 c           75 g           36 t
ORIGIN      
        1 gcataggcga catactgtac ggaacgcgct atgccataga caaagccgca atgcgcaaca
       61 tcgccccctt gcccgccgag ggcaaattcg ccgccatcgg cggcttgggc agcgtggcgg
      121 gctttgaaaa gaatacgcgc gaagccgttg accggtggat acaggaaaat cccaatgccg
      181 ccgaaaccgt cgaagccgtc ttcaacgttg ccgctgccgc caaagtcgcg aagctggcaa
      241 aggcggcga
//
LOCUS       DAWFJR010000229          248 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.229, whole genome shotgun sequence.
ACCESSION   DAWFJR010000229 DAWFJR010000000
VERSION     DAWFJR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.229"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
BASE COUNT           62 a           65 c           50 g           71 t
ORIGIN      
        1 agtggattaa atttatagtg gattaacaaa aaccagtaca gcgttggctc gccttagctc
       61 aaagagaacg attctctaag gtgctgaagc accaagtgaa tcggttccgt actatctgta
      121 ctgtctgcgg cttcgccgcc ttgtcccgat ttttgttaat cctctatacc atgtcaattc
      181 agcctttatt tcgcgggcaa agcccacgct acacccactt tctagaagta cattaggctg
      241 ccactccg
//
LOCUS       DAWFJR010000230          239 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain HB024750
            SAMN37720406-rid20221533.denovo.230, whole genome shotgun sequence.
ACCESSION   DAWFJR010000230 DAWFJR010000000
VERSION     DAWFJR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720406
            Sequence Read Archive: SRR26318854
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985328.1
            Genome Coverage       :: 518x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/15/2023 22:02:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,074
            Genes (coding)                    :: 1,956
            CDSs (with protein)               :: 1,956
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 66 of 118
            Pseudo Genes (internal stop)      :: 15 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720406-rid20221533.denovo.230"
                     /strain="HB024750"
                     /isolation_source="Lyophilized powder resuscitation"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="1980"
                     /collected_by="Suzhou CDC"
     gene            36..>239
                     /locus_tag="WGR04_002135"
     CDS             36..>239
                     /locus_tag="WGR04_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0795606.1"
                     /translation="MLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFLGMTRL
                     PTFLCNDVVKNPQVEKYLADYQAH"
BASE COUNT           66 a           51 c           60 g           62 t
ORIGIN      
        1 acggcacagg cggcttgttg caaggcaaaa aacatatgct ttcactgact tggaatgcgc
       61 cgattgaggc gtttacccgc gaaggcgatt tctttgaagg caaaggcgtt gatgttttgt
      121 atatgcactt ccacaaagcc aacgagtttt tgggtatgac ccgcctgccg acattcttat
      181 gtaacgatgt ggttaaaaat ccgcaagtgg aaaaatactt ggcagattat caggcacac
//
